LOCUS       DAFEPQ010000001       107623 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.001, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000001
VERSION     DAFEPQ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 107623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 107623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..107623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.001"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(98..1228)
                     /locus_tag="LP543_000001"
     CDS             complement(98..1228)
                     /locus_tag="LP543_000001"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBL2320204.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDRLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="LP543_000002"
     CDS             complement(1877..3079)
                     /locus_tag="LP543_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL2320205.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="LP543_000003"
     CDS             complement(3139..3342)
                     /locus_tag="LP543_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HBL2320206.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="LP543_000004"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="LP543_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="LP543_000005"
     CDS             4879..5691
                     /locus_tag="LP543_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2320207.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6715..6867)
                     /locus_tag="LP543_000006"
     CDS             complement(6715..6867)
                     /locus_tag="LP543_000006"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBL2320208.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7058..7936)
                     /locus_tag="LP543_000007"
     CDS             complement(7058..7936)
                     /locus_tag="LP543_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL2320209.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7975..9093)
                     /locus_tag="LP543_000008"
     CDS             complement(7975..9093)
                     /locus_tag="LP543_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBL2320210.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9090..11189)
                     /locus_tag="LP543_000009"
     CDS             complement(9090..11189)
                     /locus_tag="LP543_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBL2320211.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11240..12061)
                     /locus_tag="LP543_000010"
     CDS             complement(11240..12061)
                     /locus_tag="LP543_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL2320212.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12058..12867)
                     /locus_tag="LP543_000011"
     CDS             complement(12058..12867)
                     /locus_tag="LP543_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2320213.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12888..13382)
                     /locus_tag="LP543_000012"
     CDS             complement(12888..13382)
                     /locus_tag="LP543_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2320214.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13394..13882)
                     /locus_tag="LP543_000013"
     CDS             complement(13394..13882)
                     /locus_tag="LP543_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2320215.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13959..16655)
                     /locus_tag="LP543_000014"
     CDS             complement(13959..16655)
                     /locus_tag="LP543_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL2320216.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17083..18156)
                     /locus_tag="LP543_000015"
     CDS             complement(17083..18156)
                     /locus_tag="LP543_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL2320217.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18153..18980)
                     /locus_tag="LP543_000016"
     CDS             complement(18153..18980)
                     /locus_tag="LP543_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2320218.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18981..19787)
                     /locus_tag="LP543_000017"
     CDS             complement(18981..19787)
                     /locus_tag="LP543_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2320219.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19799..20884)
                     /locus_tag="LP543_000018"
     CDS             complement(19799..20884)
                     /locus_tag="LP543_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2320220.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20901..21443)
                     /locus_tag="LP543_000019"
     CDS             complement(20901..21443)
                     /locus_tag="LP543_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2320221.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21701..22690
                     /locus_tag="LP543_000020"
     CDS             21701..22690
                     /locus_tag="LP543_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBL2320222.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22687..23424
                     /locus_tag="LP543_000021"
     CDS             22687..23424
                     /locus_tag="LP543_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBL2320223.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23421..23972
                     /gene="coaC"
                     /locus_tag="LP543_000022"
     CDS             23421..23972
                     /gene="coaC"
                     /locus_tag="LP543_000022"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBL2320224.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTVIEQVMLTLNERNA"
     gene            23988..24620
                     /locus_tag="LP543_000023"
     CDS             23988..24620
                     /locus_tag="LP543_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL2320225.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24705..25352
                     /locus_tag="LP543_000024"
     CDS             24705..25352
                     /locus_tag="LP543_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL2320226.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25357..26340
                     /locus_tag="LP543_000025"
     CDS             25357..26340
                     /locus_tag="LP543_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HBL2320227.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(26743..28257)
                     /locus_tag="LP543_000026"
     CDS             complement(26743..28257)
                     /locus_tag="LP543_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBL2320228.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            28395..29201
                     /locus_tag="LP543_000027"
     CDS             28395..29201
                     /locus_tag="LP543_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320229.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(29376..29945)
                     /locus_tag="LP543_000028"
     CDS             complement(29376..29945)
                     /locus_tag="LP543_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2320230.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(30028..32088)
                     /locus_tag="LP543_000029"
     CDS             complement(30028..32088)
                     /locus_tag="LP543_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HBL2320231.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            complement(32365..33843)
                     /locus_tag="LP543_000030"
     CDS             complement(32365..33843)
                     /locus_tag="LP543_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2320232.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(34141..35076)
                     /gene="murB"
                     /locus_tag="LP543_000031"
     CDS             complement(34141..35076)
                     /gene="murB"
                     /locus_tag="LP543_000031"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBL2320233.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(35149..36069)
                     /locus_tag="LP543_000032"
     CDS             complement(35149..36069)
                     /locus_tag="LP543_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL2320234.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            36182..36934
                     /gene="xth"
                     /locus_tag="LP543_000033"
     CDS             36182..36934
                     /gene="xth"
                     /locus_tag="LP543_000033"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBL2320235.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            37798..38331
                     /locus_tag="LP543_000034"
     CDS             37798..38331
                     /locus_tag="LP543_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBL2320236.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(38821..39345)
                     /locus_tag="LP543_000035"
     CDS             complement(38821..39345)
                     /locus_tag="LP543_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2320237.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(39338..39811)
                     /gene="tsaE"
                     /locus_tag="LP543_000036"
     CDS             complement(39338..39811)
                     /gene="tsaE"
                     /locus_tag="LP543_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBL2320238.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(39934..40914)
                     /gene="pta"
                     /locus_tag="LP543_000037"
     CDS             complement(39934..40914)
                     /gene="pta"
                     /locus_tag="LP543_000037"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBL2320239.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(41067..41747)
                     /locus_tag="LP543_000038"
     CDS             complement(41067..41747)
                     /locus_tag="LP543_000038"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBL2320240.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            41873..42742
                     /locus_tag="LP543_000039"
     CDS             41873..42742
                     /locus_tag="LP543_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL2320241.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            42768..43283
                     /locus_tag="LP543_000040"
     CDS             42768..43283
                     /locus_tag="LP543_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320242.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(43330..43923)
                     /locus_tag="LP543_000041"
     CDS             complement(43330..43923)
                     /locus_tag="LP543_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2320243.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(43948..44127)
                     /locus_tag="LP543_000042"
     CDS             complement(43948..44127)
                     /locus_tag="LP543_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320244.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(44204..44713)
                     /locus_tag="LP543_000043"
     CDS             complement(44204..44713)
                     /locus_tag="LP543_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320245.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(44809..45189)
                     /locus_tag="LP543_000044"
     CDS             complement(44809..45189)
                     /locus_tag="LP543_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL2320246.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            45519..46268
                     /locus_tag="LP543_000045"
     CDS             45519..46268
                     /locus_tag="LP543_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBL2320247.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(46452..47729)
                     /locus_tag="LP543_000046"
     CDS             complement(46452..47729)
                     /locus_tag="LP543_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320248.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(47808..48452)
                     /gene="pcp"
                     /locus_tag="LP543_000047"
     CDS             complement(47808..48452)
                     /gene="pcp"
                     /locus_tag="LP543_000047"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBL2320249.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVSFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(48651..49595)
                     /locus_tag="LP543_000048"
     CDS             complement(48651..49595)
                     /locus_tag="LP543_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBL2320250.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(49597..50283)
                     /locus_tag="LP543_000049"
     CDS             complement(49597..50283)
                     /locus_tag="LP543_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBL2320251.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            50757..51077
                     /locus_tag="LP543_000050"
     CDS             50757..51077
                     /locus_tag="LP543_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBL2320252.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            51231..52136
                     /locus_tag="LP543_000051"
     CDS             51231..52136
                     /locus_tag="LP543_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2320253.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            52133..52906
                     /locus_tag="LP543_000052"
     CDS             52133..52906
                     /locus_tag="LP543_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL2320254.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(53087..54511)
                     /locus_tag="LP543_000053"
     CDS             complement(53087..54511)
                     /locus_tag="LP543_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2320255.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            54722..55168
                     /gene="nrdI"
                     /locus_tag="LP543_000054"
     CDS             54722..55168
                     /gene="nrdI"
                     /locus_tag="LP543_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBL2320256.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(55233..57215)
                     /locus_tag="LP543_000055"
     CDS             complement(55233..57215)
                     /locus_tag="LP543_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL2320257.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(57229..58125)
                     /gene="murQ"
                     /locus_tag="LP543_000056"
     CDS             complement(57229..58125)
                     /gene="murQ"
                     /locus_tag="LP543_000056"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBL2320258.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(58128..59189)
                     /locus_tag="LP543_000057"
     CDS             complement(58128..59189)
                     /locus_tag="LP543_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBL2320259.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(59400..59966)
                     /locus_tag="LP543_000058"
     CDS             complement(59400..59966)
                     /locus_tag="LP543_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL2320260.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(59994..61496)
                     /gene="eat(A)"
                     /locus_tag="LP543_000059"
     CDS             complement(59994..61496)
                     /gene="eat(A)"
                     /locus_tag="LP543_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBL2320261.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(61754..62875)
                     /locus_tag="LP543_000060"
     CDS             complement(61754..62875)
                     /locus_tag="LP543_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL2320262.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            63001..63942
                     /locus_tag="LP543_000061"
     CDS             63001..63942
                     /locus_tag="LP543_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBL2320263.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(64092..64988)
                     /locus_tag="LP543_000062"
     CDS             complement(64092..64988)
                     /locus_tag="LP543_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBL2320264.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            65257..66597
                     /locus_tag="LP543_000063"
     CDS             65257..66597
                     /locus_tag="LP543_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2320265.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            66815..67258
                     /locus_tag="LP543_000064"
     CDS             66815..67258
                     /locus_tag="LP543_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBL2320266.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(67762..69180)
                     /gene="nhaC"
                     /locus_tag="LP543_000065"
     CDS             complement(67762..69180)
                     /gene="nhaC"
                     /locus_tag="LP543_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBL2320267.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(69583..70806)
                     /locus_tag="LP543_000066"
     CDS             complement(69583..70806)
                     /locus_tag="LP543_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBL2320268.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(70837..71676)
                     /locus_tag="LP543_000067"
     CDS             complement(70837..71676)
                     /locus_tag="LP543_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBL2320269.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(71693..72373)
                     /locus_tag="LP543_000068"
     CDS             complement(71693..72373)
                     /locus_tag="LP543_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2320270.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(72375..73448)
                     /locus_tag="LP543_000069"
     CDS             complement(72375..73448)
                     /locus_tag="LP543_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2320271.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(73504..73742)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(73785..74018)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(74095..74766)
                     /locus_tag="LP543_000070"
     CDS             complement(74095..74766)
                     /locus_tag="LP543_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL2320272.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(74853..77531)
                     /gene="mgtA"
                     /locus_tag="LP543_000071"
     CDS             complement(74853..77531)
                     /gene="mgtA"
                     /locus_tag="LP543_000071"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBL2320273.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            78190..78627
                     /gene="ndk"
                     /locus_tag="LP543_000072"
     CDS             78190..78627
                     /gene="ndk"
                     /locus_tag="LP543_000072"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBL2320274.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(78649..79515)
                     /locus_tag="LP543_000073"
                     /pseudo
     CDS             complement(78649..79515)
                     /locus_tag="LP543_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(79675..81768)
                     /locus_tag="LP543_000074"
     CDS             complement(79675..81768)
                     /locus_tag="LP543_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL2320275.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(82517..83125)
                     /locus_tag="LP543_000075"
     CDS             complement(82517..83125)
                     /locus_tag="LP543_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320276.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLITAYSEKVDKKVVADRLAEWKKTNEQVKKTTVSSQK
                     NESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(83284..84681)
                     /locus_tag="LP543_000076"
     CDS             complement(83284..84681)
                     /locus_tag="LP543_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL2320277.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(84713..85684)
                     /locus_tag="LP543_000077"
     CDS             complement(84713..85684)
                     /locus_tag="LP543_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2320278.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            86058..86483
                     /locus_tag="LP543_000078"
     CDS             86058..86483
                     /locus_tag="LP543_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320279.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(86971..87672)
                     /locus_tag="LP543_000079"
     CDS             complement(86971..87672)
                     /locus_tag="LP543_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL2320280.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(87688..88128)
                     /locus_tag="LP543_000080"
     CDS             complement(87688..88128)
                     /locus_tag="LP543_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBL2320281.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(88128..88703)
                     /locus_tag="LP543_000081"
     CDS             complement(88128..88703)
                     /locus_tag="LP543_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HBL2320282.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(89551..90030)
                     /locus_tag="LP543_000082"
                     /pseudo
     CDS             complement(89551..90030)
                     /locus_tag="LP543_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(90189..90890)
                     /locus_tag="LP543_000083"
     CDS             complement(90189..90890)
                     /locus_tag="LP543_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2320283.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(90998..92407)
                     /locus_tag="LP543_000084"
     CDS             complement(90998..92407)
                     /locus_tag="LP543_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBL2320284.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(92455..94320)
                     /locus_tag="LP543_000085"
     CDS             complement(92455..94320)
                     /locus_tag="LP543_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL2320285.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAEADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHRGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(94441..95292)
                     /locus_tag="LP543_000086"
     CDS             complement(94441..95292)
                     /locus_tag="LP543_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL2320286.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(95602..96315)
                     /locus_tag="LP543_000087"
     CDS             complement(95602..96315)
                     /locus_tag="LP543_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBL2320287.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(96383..96709)
                     /locus_tag="LP543_000088"
     CDS             complement(96383..96709)
                     /locus_tag="LP543_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBL2320288.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(96832..97059)
                     /locus_tag="LP543_000089"
     CDS             complement(96832..97059)
                     /locus_tag="LP543_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBL2320289.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(97381..98634)
                     /locus_tag="LP543_000090"
     CDS             complement(97381..98634)
                     /locus_tag="LP543_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL2320290.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            98695..99435
                     /locus_tag="LP543_000091"
     CDS             98695..99435
                     /locus_tag="LP543_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2320291.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(99521..100279)
                     /locus_tag="LP543_000092"
     CDS             complement(99521..100279)
                     /locus_tag="LP543_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBL2320292.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(100276..101304)
                     /locus_tag="LP543_000093"
                     /pseudo
     CDS             complement(100276..101304)
                     /locus_tag="LP543_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(101324..104491)
                     /locus_tag="LP543_000094"
     CDS             complement(101324..104491)
                     /locus_tag="LP543_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320293.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(104715..105599)
                     /locus_tag="LP543_000095"
     CDS             complement(104715..105599)
                     /locus_tag="LP543_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBL2320294.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(105694..106131)
                     /locus_tag="LP543_000096"
     CDS             complement(105694..106131)
                     /locus_tag="LP543_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320295.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(106348..107259)
                     /locus_tag="LP543_000097"
     CDS             complement(106348..107259)
                     /locus_tag="LP543_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBL2320296.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT        31368 a        22453 c        18439 g        35363 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatc
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc
     5821 tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga
     5881 cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca
     5941 taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg
     6001 gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca
     6061 acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat
     6121 tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt
     6181 tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga
     6241 aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc
     6301 aaattatttt tctctaataa gaaaaacaga aaaggtaata agatacaaca gtgatgattc
     6361 ctaataaaga aaagactaca ataatatttt tctaacttat cgccttcaaa gaaatagttt
     6421 cttatttttc actaaaataa aaaatttatt gcaaaaaaaa tagaagtgac aaaattatca
     6481 atcatttcaa ggtaatataa ctacctgagt ttattatcga ttaaaatgct aactacattt
     6541 gcaagagaag cgagagttaa aggtaaatat agaatttcta cttttttagc aatagaaata
     6601 ggaataaaat atttaaaatg tatagaaact ttcttttttt ttcttgctat ctttttataa
     6661 tcacaactct ctataataga attacgattt attttatacg gctgaaaaaa tctatcacca
     6721 aaaaaattca aacattaagc caaggattat gaagtcaaca tttcgaaaaa tgaacataca
     6781 ttggtaagca actataacta ttaccacaga cataaagtaa cacaatatat tactatttaa
     6841 gtgtacgcca ccagtgaata tcctcaaggt atacagcgca caacaaaaag aaaaaaggaa
     6901 acgcagaagc atttcctttc agtgatttaa tgaaaacaac acgatttgac ttagagcctt
     6961 tttatttgaa ttcattttcg ctttatccaa cttcatttga tcatttgatt actcggttaa
     7021 atacttttga catgagtatc aatgaaacat tcattaatta tggttgctta tttcttaccg
     7081 cttttctggc caatgctaat ccgccgatta ttccggcatt atcacctaat tcgggcgtaa
     7141 taatgtagct atcaattggt ggtatttcaa catatccatt tataagctca acaaatttat
     7201 cctgaacttt tttcttcatg tgttcttgtt tcattacacc accgccaaaa ataattctat
     7261 ctggcgaaaa catcagtgtt gtattgtatg cacattgggc aaggtaaaag gcttcaatct
     7321 gccaaaacga atcgtcagcc aataaattct ggccttttac acccaaccgt ttttcaatcg
     7381 ctggtcctgc tgccatcccc tctaaacaat cttggtggaa gggacagttt cctgaaaaat
     7441 tgtcctttgg atgtcgcttg atcaacatat gccccatttc cggatgactg aacccttcca
     7501 cgaaagtccc attgaacaag gcaccacctc cgatacctgt tcccacagtg aagtatgcaa
     7561 cacctgctaa ccctttgcca gaaccagcaa catattcacc atagcaagcc gcattgacat
     7621 ctgttgtcca ttcaatcgga atattgaaat attgctttag cgttcctaca aaattgaaat
     7681 tttgccaagc aagttttggt gttgatgtga tatgaccgaa actcgttgat ttgcattgaa
     7741 tatcaattgg accaaagcag ccgaccccga tgcctgccaa ttgttcctta aaaggtttaa
     7801 aaaaatcaat gacctgcttc attgtttcaa ctggagttgt tgtgggaaaa ctgactcttt
     7861 ccaaaatcgt taattcctca gtaccgactg cacagacaaa tttagtccct ccagcttcaa
     7921 ttgccccata tatcatcata ggttcctccc ttcgaatatg tgaaaaacac ttcattaacg
     7981 cggcaagcgt tctcgatcag tgaaatgacg tttagtattt cctgaagctt ctgttgaata
     8041 ccagtaagcg acactagcaa catcgtcttg ccgctcaaat aagcgaacat catcgttgcc
     8101 gatttgttgt aaagtgaccg ataaatcttc ggtaaaacag atggggtctt gaagatgcca
     8161 acgataaaga ccatgtcgtg gcattgcgtc gtcaccaaag gcatgaacgg cattcaattt
     8221 tcccgtttca aaacgatctc ttgtggcatc tctatttgat tggtaaggat accctaaaaa
     8281 ggctgttgaa tagtttttcg catgtggacg tccgaactca tcgtactcat gaaaggccca
     8341 cgccccgcca aaataatctt ctgaacctgt tgaggttacg gtcggatact cactatcccc
     8401 atcaatgtag aatttaaact cgccttcgcc ccaccaataa cgttctaggc aggtaagcgc
     8461 aaggtaagta cccacgtaat agccttgacc ctttaatcga tcaattaact tataatcctc
     8521 ctgtaatttg gtaatgcgtt cccgatgcca atttgcgtga aaatataggc tattttcttc
     8581 caattcgtcc accaaagcgt aatttatcgt ataaaaaaat gaggacatat ccttcggatg
     8641 taaattctcg atcgtaattt tagcggatgt ttggaaaggc atttcgaaat aggaattaaa
     8701 gccccctgta ggattaacca ctatcggcaa cgagttcacc tcgcaacgct ctccaaaccc
     8761 attacaaaag aaatccccta aaggactttc aacagcaggg ctatccgacc catcccaata
     8821 aattcttaag attaaatcac gtaaaacaaa actgccaaat tctgttcgat cacgaatcgt
     8881 catccacata tgacgaatta ttcccgttcc agtaatatcc gcaatcacag tttcttcacc
     8941 agaagtcagc ggaattgcgc cacgaccttt gcggcttggt cctagctcgc ttggcaacat
     9001 cgcagcttta cctttctcgc ctgttggatt ttcgggagtg attgctcgac tctggatttt
     9061 tttaaattgg gtaatatttt ttagtatact catctttttc tcctttgaat gtgaatcgtt
     9121 tttccctttt ctacgtgaag aatttttttc gtatcactta ttttcaatgg cttttctgac
     9181 aaatttgaaa tactcaactc ggattgcgta cattcaatcg aaatgaaggc ttgaccgaac
     9241 ttcaaattct tccaagaaaa cgttaaatta tcaacaggat caatttgcaa tactcctttt
     9301 ttgaatttca atccgaaata ttgactgatc attaggctga caaaaagacc cgatgcccag
     9361 ccgcatttgc cgcagccaaa gtctcgcacg acttctcctt gttgagcctc taagcgatac
     9421 ggccaccacc accacgaacc gtctaaatct gccaaacaaa ttagttcttc taatcgggtt
     9481 acgaaagctt gagggttgtg cagctcgctc attaaaacag tgataaagcc aggaaaagtg
     9541 gcgccggatt ctaatcccca agtgatgcct ttgatttcct cgctataggt tggattgaaa
     9601 gaactagcag aaaaggccat ggtgttctga tataaatgat ctttattatc taaaaattga
     9661 taaaaaggca tcaacgtagt gtctgattct tctccatcat gcatcaataa attaattttt
     9721 ccatccacaa tcacatcact taaaaagatt aacccttgct caagaatcgg tttttgatag
     9781 tgcttcactg aataggttgt tttctcctca tcaccgattc cctccaaaaa ttgctcacca
     9841 aaaggaccat cgatagtcat ttgtttacaa attgattcct ttagtttaca ggcaatgttt
     9901 ttgtatcgct tgctaagact cagttgttct agggcttcaa aaatttctgc aagtccaata
     9961 caggccttcc aaagacaaag attgctgccg gtatgatact ttccaagtgc aaaggcgtct
    10021 gataaccaaa cagatggaaa aagcattggt tcccctgacc gacgttgagc taatatgtcc
    10081 tcaataattc ttgaagcatt cagtagaacc tgcggacacc gctcaaagaa ttttgtatcc
    10141 ccagtatgtt caaaataaag actagccagt aacgcactgg ataacgaatt actcagagaa
    10201 tgattgatac ctcctgtaaa ttttgaacca ggaaacttaa cgccatattg atcaaaccat
    10261 agaaccgttt tttgacataa ctccggttca aacatggtga atggcaatga tgcgtaatac
    10321 atatccttat tccaaattcg attagtagca ggatagctgc cccaattgct gccgacgacc
    10381 tgtccctgtc gattcatacc gaaactagaa aagctttgat aaagtgcacg acgatagaga
    10441 tggacaactc tatcgtttgg cattgacagc tgcccaaata gctgagcaaa atagttcaat
    10501 gtttccttca gccatttttc gatatcagat tcctgaaaag tcattacttc ttcaaaagca
    10561 tttgggtcca caaaagcaat ggcgaagctt gccacttcct gttgtgcgat cgtgtcctta
    10621 tttccttttt gagaaatcaa cacattttgc tgatccgaat acttctcttg atacgaaggc
    10681 acatctttca ctgaaacttg aaggctttcg tgtgtttcat tcacaatgta aacctgttga
    10741 acaagcatac ttaaacgctt attagccagt atgggagcaa aggatactac aatgacctta
    10801 aacgtcgaat aatgatgaat atatctaggc aaacagtctt gaatcacatc gacttcaata
    10861 cgatgatccg attctgacaa atggtagaca atcccgttta cttcaacctt taaggataac
    10921 gaattggttt gagagagctg tttattgacc caaacacctg gtttttcctc agttagtaaa
    10981 ttttctcgat aaaaggtcaa cgtttgaatc gtcccgcaaa catcaaggtt tgctaatgtc
    11041 ttttgattac ttacatcgaa ggtcaatccc tttacatcag gaatattccg atagtagctg
    11101 tattcgtttg aagtagtcac atttccgtcg tttaactcag cattaaaaaa tgggtgtttg
    11161 ggtgaataaa ttacttcatt aatagtcatc ttttccctcc ttgctaaaaa aactgcagcc
    11221 tagtagctgc agcttcctac tatgcgattc caagtaatga caataaaatt cccaagacga
    11281 ccattccaaa aataattttg atgacgccga cttttttatc tagtaatttg aaacagctca
    11341 atgttaccaa taacggtaca attcctttaa aaatactatc gaggatttct tggacattca
    11401 ttagttcttc gcccttcatt gaaaagctca aaacggtatt gaatttgacc atactgatcg
    11461 tcattgcacc taccatgatc agacccaaga tactagcagc ttttgtcaga atttcaatta
    11521 acccgttcgc atacagcttt tcaatatatt tagagcctaa tgaatagcct aaaacgccgc
    11581 catagtatcg catcaagtaa ttaggtatat tgaacataac taaaaagata attggtgcca
    11641 aaatatttcc ggcattggct aagttgatcg ctagtcccgc ggtaacgaca cgccaaactc
    11701 cccagaacaa tgaatcgcca attccagcca atggtcccat caaactcgtt ttgatcgcgt
    11761 tgatactttc cacgtcaaaa ccagcttttt cagaattttc cttctccatc gatgccgcaa
    11821 tccccatgac aaaggacgcg caaggaatgg tcgtcgaaaa atactgtgta tgacgttcca
    11881 gcgccgcagc tcgatcttct tcacttttgt agaaatgatt aatgaagggc atcatagaat
    11941 aggcgaaacc accgccgccc atcgtcaccg gattcaccga agcatacata gtcatcgagc
    12001 gccaaaacat cttccgaacc aattttttct catctttaga aattccatca gctaatttca
    12061 ttgaaagaaa tcctcctctt cttggtcaag cgttgcattt acagcagcat cgctttgaac
    12121 tgcatgaaca gatatatttt ttagttgtag atctctttgg gctataacaa ttgcaataat
    12181 cgttccaact gcggcaattg caatcaatgg taactcgaga tacgccataa caacggcccc
    12241 aaagaaaaac cacacagcaa tatttttact ccaaagcatc cgtaacagca tcgccatccc
    12301 aactgccggc agcagatttc cagcgactgt taatccgttc atgatgacct ctggaatatt
    12361 atccaagacg cttttgacaa catctgctcc aaataaaacg gcaaagaatg gaataagagc
    12421 ataaagcgca tagtggacaa accacataaa gaagtgcaga ttgatgattc ctttttgatc
    12481 accagatttt gcaaatttga aaaatttagc agccatcggt ccacatacaa aagaaaaaat
    12541 catattcatt aactgtaatc ctaatactgc tagtggaaag acaatgggaa taatcgcatc
    12601 cacgtttcct cccattttga ttgcgaacgc tgtagccagc ccagcagcca aagaaggctc
    12661 cgccgtacta gagattccga tattgacgac acccataaag atggcttcta gtgacgcccc
    12721 gacaatcaac cccgtctgca aatcccccat caacgctcct gctaacggag caacaacaat
    12781 cggacgactc agcatggaca tcccgaacat ttcatgtcca gcaactccaa taaaaacggc
    12841 caaagcgact acaaatgcat catacataat ttttctcctt cctttttcta aaaatcactc
    12901 aaaatagaga tagctgaacg tttttccatc gccggtgtag gctgaaaaac ggtatctgga
    12961 taaagttcaa ctaattcttt taagtcttga atttctgacg tcgttaacat cacttccttt
    13021 gataaggttt tcttatcttc cgatggcggt gtcattttcc cagcattccc cacgttcatt
    13081 aattcgattt ccgttgggaa agctttcgca acttttaccg catctttgac attagggact
    13141 aaaaccatca agcgcatgtc cttcgcccgc ggatctttta acaaatgaat cgcgtcatca
    13201 actgttttaa taatgacttt gacattcgat ggtgcagcca tcttcaatgt catcttttga
    13261 atatcgttgg tagccgcctc atcgcttgct actactaacc cggttaaatt tagactcttt
    13321 gtccaagtca tcgctacttg tccatgaatt aatcgttcat caattcgcac tgctttaatc
    13381 atttttcttc ctcctaaaaa aaatcttcct cttcatttac tacatgtacc tcatcaagaa
    13441 tggtcatctt taattcctgt tggcatttat ttaccagttc ctgaatatcc tcctttgtta
    13501 attccttggt tgccaatact atttcgagca gaattggcag attaaagcca gacaaaatag
    13561 ttacagcaat ctctttctcc aaaaaggttt tggtaatctt ctgattgaca cttccgccaa
    13621 ataaatcggt aaaaacaaaa atctgatcct gctcgttcac ctggtttaca aatacattca
    13681 actcctgttc aaaatcacac tcatccacat aagcattaat caccgtaatc tcttgaacct
    13741 tccccgtcag taattgaata ctgctaagaa agcctgaagc taagtgaccg tgagtcgcta
    13801 ttagaatttt cttcatatgg ttaattcctt tctttctgtt cacttttata taagcaattc
    13861 ctgtgccaac ttttaaaaac attggtttaa tcacgttaat tcacattaaa aaaagtgtct
    13921 aacctaaaaa aaacagatta gacacttttt tagacacttt atttttgaat atacggtcct
    13981 aataattcat agagcagcga aagctcataa gcattgactt taaaattata tttcatctcc
    14041 attgattgaa aaatacttct agaaacataa taaaattcat cttcatctgg tttcaaatca
    14101 taattcggtt cttcagattt ttctgtccga gccaagaata aacgttccat catcaatgcc
    14161 gtatgcatat agagatttag ttttacacgg ccatttaaag tgaaatgata gtaattttca
    14221 tatttagaaa tgactgtttc cacttcactc atcacgatcg ttgggttcag aaagttcaaa
    14281 cggtccgcaa ttccttccaa cgaaaagaat tttactagct cctgaatcaa gcggttaaat
    14341 tctgccggct tcatttcagg actcaaaata tgctccaaat tggctgctcc atcaccgtct
    14401 aagatgtcat aaacattaat attaggaatt gaaaaggaac gtggaagatc cgaagtcgtg
    14461 atgaccaatt ttgtcttgtc aaaataagtt tcatcatttt ggtcgattaa ttcacgtagt
    14521 tctctgtagt ccttcgtgaa aacttcgatg gattcatccg ataaatgatg acgaaaaaca
    14581 tctttcaatt tgtccgaaat ccccaaaccc gacatacagg aaatgataat gttgctactt
    14641 tgagaaaagc cttcaaagta ttgaacattg attgaatact tgtctgctgc atcctccgca
    14701 atctgtttaa atggcgcatt catcgtcatc ttcaagccaa tatcaagtgc aatggaagtc
    14761 gtcaaattat tcagaagaag taactcccct tttaaatcgt tcttgatctg tgtgtaaatt
    14821 tgatttaaag agcccatatc tacgatcagc actagactat ctgtattcac ttgccgtttc
    14881 acgaaattcc ttgtcgattc gacaatctct gacacattac tctctactgg catatccaac
    14941 gcttcaaaaa cataattgcc gcataattga ttcactactg cttgaatgct gctagccgta
    15001 ctatctccgt gagcaagcaa caggcccttc agctcgatgg attcatctac ataatcagat
    15061 aaagtcacga caataagcag tcttagaaag ttctcgtcct tttctgagag cggtggtaaa
    15121 tgctccaaga actgattaca taagtaagtc gtccgaggta gatttgcagc taatgtacga
    15181 tttatcccgg ttaagtctgt cttatagctt ggggaaatga cataggatct gctaataatt
    15241 tctgcaacag gtccactcgt cgtcaatccg tacgtttttc ctaaaacgga tacccagacc
    15301 tttctaaaca tctgaatcat caattttcta ctcattgata aaaaagaatc cttatcaatg
    15361 tgatgcacca aatgctgtac ttcattcttt atcgaagaaa aattttcatt gaaattacct
    15421 tcatttatca gctgagtaat tttttccaaa atcgaatcat aatcaaacgg tttgattcga
    15481 tccgttttta tcttttgatt tttctttatc aataaactag caatcggcaa gctttctact
    15541 tcggattcca gcatatttaa ctgaatcaag atctcatttt tcccaatcgt taatggttct
    15601 tcttgtgttc gactatacac atccgcacaa ctgattttaa tgatattctt cagcttacca
    15661 atattcccac taaagggatg agataacatt aaagacaagg cttctcttga aacaataata
    15721 tctttttgta aaacatccgc ttcattttga aaaaataatt gaatcagctc taatcgttct
    15781 cgaatggggc gatcttcaaa agttggcaac ataacactaa tttgaatacg ccgatcaaag
    15841 gtgtctaata aataattttc acctttttcc gtcgtggcga aaataaaacg aaccttagca
    15901 gaatgccaat ttacattttc accgactgga cgatagtttc cagtatcaat aaatagaaag
    15961 agcttttctt gattttcttt cgataggcga tggacctcat cgagaaaaag atagccgttg
    16021 ttggccgttg ccaataaacc atcggtatcc ttttcagccc ccgtaaaggc ccccttcttg
    16081 tatccaaaca gtgttgccga aagcagttcg ggattgtccg cataatcggc acagtttaag
    16141 atagcaaatg gtgcatcttt ttcaataatt tgctcatgga tagcgtattc aaaaattttt
    16201 gtggctaaaa aactttttcc aacacccgtg gctccattta ttaatacact gagtccgtta
    16261 ggcggatatt tgacagcggc tatacatttt tgaatgacct ctctcagact tccgtcatga
    16321 ccaactacag aatgaaaggc ttcgtttatt tgctccgtat tatctacaac cgcatattca
    16381 tctaaaggct tccacaaaac aggctttccc ggtaatttct cgaccttttt atcttccact
    16441 aatctcgtca aataatggct ggcattctga cgagagatac ctagattctt cgaaattttc
    16501 tcggcagtga cagaagacat attccctgtt agtcgtatca gctcatcaaa aactcgatct
    16561 ttagcgctct ttttcatttt ttcctccagt ttaatgtgtc taaaagcata ataaactatc
    16621 ggctgcaata tgtctattct atatttgtat tccaattttt actttgaaac acaaatagtc
    16681 gaatatttgc ctcagatatc tgacaaaata atttatctac caatttagtt tttatctaat
    16741 atgattttta tgttttttca tagctacaaa tattgaactg gattcatata ttagttttat
    16801 taatagatta cagaagggtt aagtatattt gcatagtttc caattacttt atccttctgt
    16861 aactttttca atcctagatt tactagcccg tctcgggccc tattttacaa tggttttgtc
    16921 cacttttctc tggtagaaaa acgtggtaga aaaaataaaa taacacaaaa aaagcggcgt
    16981 ttcatccgct tttggtagaa gattggaaaa ctcagataga aaaaggacga tttttagctc
    17041 tttagaacgt tgataagtcc ccattcttac tgagttttgc cattatctcc tatacatctt
    17101 gaattctaag aataggtcat tatagatgcc taggtatttc gatcctagat ctttgtagac
    17161 ttcaagatgg gagatcgtgt catcagatgg atagaattgt tcgtccgacg aaatgtcttt
    17221 tggcaataac gcttttgctt cttcatttgg tgtagaataa ccaatatact ctgcattttg
    17281 tgccgcattt tccggttcta gcataaagtt gatgaaatca taggcacctt ctttattttt
    17341 cacggttttt ggcataacga tattatcaaa ccaaagattg gatccttctg gtgggatcac
    17401 ataatgtaaa tgttcatttt cccacatcat atcggcagct tcgccagaaa atgtcactgc
    17461 cacggaggct tcttcattga tcatatacat tttgatctca tctgccacga ttgctttgac
    17521 atttggtgtt aaatgattca atttatttgc tgcttctgtc agctgtgcca tattcttact
    17581 attcaaagaa tagcctaaac tattcaaaga aagccccata acttctcgtg caccgtcaat
    17641 cagcatcaaa ctgtctttta attccggctt ccacaaatca tcccaatgct taatttgatt
    17701 tgcatcaaag aatttatcgt tatatacgat ccctaaagtt ccccaaaaat atggaatcga
    17761 atactgatta tctggatcaa aatccaagtc taaaaatcgt ggatcaatat tatttaatcc
    17821 tttgatcttc gaatgatcta atggaagtac tagtttttct ttgatcattt tttggatcat
    17881 atattcactt ggtatagcga tgtcgtagtt tgtcccacct tgctggattt ttgtgaacat
    17941 cgcttcatta gaatcaaatg tttcataatt cactttgtat ccatattctt tttcaaattt
    18001 cgtgatcaaa ctaggatcga tataatctcc ccagttatag atggtaagta cctttgctcc
    18061 actcatgcca cttgtttttt caagttgacg aacaccaagc agcagcaccg caataatgat
    18121 gagaatacct aaaaacagag attgtaattt tttcatcgca gccgtgccac cccctcgcgt
    18181 actcttctgt ttttcttcag ccgttttgga cggttttctt tactaataaa gtagtatcca
    18241 atgaccaata tcatggagaa caagaaaact aacgcactta gcgcattgat ttctaaactg
    18301 attccttgtc ttgcacgaga atagatctct actgagagcg tcgtaaagcc atttcctgtg
    18361 acgaaaaatg taaccgcaaa atcatcaaga gaataggtaa atgccataaa atatccagct
    18421 atgattcctg gagaaagata aggcaaaatg atatttttga ctacttggaa attattagca
    18481 ccaaggtctc tagccgcatc gaccattgaa tcgttcattt cctgcaattt tggcaagacc
    18541 atcaagacga cgattggaat gctgaaagca atatgggaaa gcaatacact ctgaaaacct
    18601 aatccgaatc ctagtagtgt aaagaaaatc aagaaactag caccgataat aacatcagga
    18661 gagactaata agatgttatt catgctcaac aacgctgtcc gtgcacgtcg ttttttcgtg
    18721 taatagattc ccatagcccc aaatgtcccg atgatcgtgg cgatcaatgc agataagaaa
    18781 gccagcatga acgtattcaa tacgataatg atcaaacgtg tatcttcaaa aacagcttca
    18841 taattttccc aagtaaatcc tgtaaagctg ttcatcgttc ctccttcatt gaaggaataa
    18901 aaaatcagat agaagatcgg tgcatataag aataaaaaga cgatccctaa ataaagatac
    18961 gaccatttaa attttttcat ttatgagcga cctccttttt tcttctcgcc tgtcaggagc
    19021 atcacgatga acatagcaat gattaaaatc acaccgattg tagaacccat cccccagttt
    19081 tgtgtaacca agaaatgctg ttcgatagcc gttcctaaag tgatcacccg atttccgcca
    19141 atcagacgag tcagcatgaa taaagaaaga gaaggaataa atactgcttg gacaccactt
    19201 ttcacaccat ttaatgataa cggaaaaatc actcgtcgga aggtctcaaa gctattcgcg
    19261 cctaaatctc tactagcact gatcaaagaa ggattcatct cttccaatgc attgaaaatt
    19321 ggcataatca taaatggaat ctcgatatac gttgccacga acagaaaact gaagtctgtg
    19381 aataatatct gtttcggccc gatcccgata aaatcaaaga aatgattcac gctgccgtgc
    19441 acactgaaaa tcccaataaa tgcgtaagct ttaagcagca gattcaccca tgtaggcaaa
    19501 ataatcagca tcagccataa ttgcttgtgc ttcagttttg ttaaaaaata agcagtcggg
    19561 taactgatga tcagcgtgaa aaacgtgatc aaaaatgcat accatacaga attgatcgtc
    19621 atagaaagcg atgtccccga cgtaaaataa gatagataat tattcagcgt gaattgccca
    19681 ttcatatcaa aaaaagattg ataaatgatc attaatacag gcgcgatcac gaataaaccg
    19741 agccacaaaa tataagggac ggaatagatc ctgcgcattg ttttcattct tgtcccccct
    19801 attcttcgta gctgtcaaga cgtgcatcga attcttcttc agactcgttg aaacgcatga
    19861 catggatatc ttctggctca aaggccaatc ctactttgct tccttcttta gcttttttcg
    19921 ttgaatggac catccattca ttttgctgtt catcataaca gatgatttca taatggacac
    19981 cacgaaacag ctgagtatcg accgttacga ctagttttcc tttatctggt gtcgtgatcg
    20041 tcaaatcctc tgggcgtaaa acaacttcaa ctggttcatt tagacgcatc ccgccatcga
    20101 cacattcaaa ttgcttacca acaaactcaa ctagattatc ttcgatcatt acaccgtcca
    20161 cgatattgct ctccccaaca aaatcagcaa cgaaatgatt gattggctca tcataaatat
    20221 ctacgggtgt accactttgg acgattttcc ctttattcat gacaaaaatc tcatcactca
    20281 ttgccaacgc ttcttcttgg tcatgagtaa caaaaataaa cgtgattcct aatcgttgtt
    20341 gcagatcccg cagttcatac tgcatatctg tacgtagttt caaatcaagc gccgataacg
    20401 gttcatctaa tagcaatact ttcggttcat ttacaattgc tcgtgcaatc gctacacgct
    20461 gtctttgacc accagacatt tcactaatct cccgtttttc gtatccagga agctggacca
    20521 tacgcaaagc atcttttacc ttcttttcga tatctgattt ggacattttc ttgatcttca
    20581 atccaaaagc aacgttatca aaaacattca tatgaggaaa aagtgcataa tcttggaaga
    20641 ctgtatttac ttgtcgctta ttagctggca aatcattcaa ccgtttccct tcaaaataga
    20701 tgtccccttc tgtagcttcg gtgaatccag ctataatacg taggatcgtt gttttaccac
    20761 agccggaagg accaagcagt gtataaaatt ttccttgttc gatctcaaaa cttacttttt
    20821 ttaatactgt ttcatcatca tagcgtttca ctacttgatc aaatgaaatg attggtttcc
    20881 ccacgttttt accccctata ttacaaatat gattgcgttg caacaatcaa tacttttgtt
    20941 cgttttttga gatgattcct taattgatgt tgttttgtcg cttgataata gatcgactgt
    21001 cctgcttttg ccacatagac cgtctctcct aagcttaact ctaattcccc ttctaataca
    21061 aaaataaacg tttcagataa tgaaggttca aacgtcttat attctcctgc accttcaaaa
    21121 gtcagcaaga caggctccat ttctttttca ttcgaatcag ccacgagcca ttccaattca
    21181 tacccgtttt cttcatcgaa ataaaccgta tgatcttctt ttgaataaac cacttgcaga
    21241 ttcgctgctt cttgatgaaa aaattcttct ggagtcacac ctaagacttc aagaatagaa
    21301 aagaatgttt ccattgatgg tgaacttaaa tctcgttcca actgggaaat ataccctttt
    21361 gacaaatctg tgcgttcgcc tagttcttct tgtgtcaaat tcttttggat gcgcaaattt
    21421 cttaacttct caccaatttc cataatttcc tccgaatcga agttttctaa tagtaaactt
    21481 cctgtttact accactcgta ttagtatacg aaatttattg aaaaaggcaa ccctttgtaa
    21541 aatttttttt gctgaatatt tctttatctt ttggaataaa ttctagttgt ttgattcttt
    21601 atttttcacc taatttttgt ctaaatttac ggaagaacaa gaataaaaga gtaggcttta
    21661 tggtatgata aaagaaaatt ttaaaaggag tgtctcacga atgaacgctt ttcgatcttt
    21721 tcaggtaata aaggaacctg ttttccatac agcaatcagt gatctgccat ttactgagtt
    21781 ggctgaaaat gaagtcttgg taaaaattgc ctattctgat gtcaattata aagatgcatt
    21841 ggcaatgagc gagtctggtc aggtgatacg aacatatccg atgactccag ggattgattt
    21901 atccggaact gtcgtccaaa gtaacaatcc acgtttttca aaagaagatc ttgtacttgc
    21961 tactggtttt gggcttggcg tcacccatcc aggcggttat agccaatatc aaaaagtacc
    22021 aggagactgg ttagttcctc taccaaaaaa tatgacgctg cgtcaagcga tgatccttgg
    22081 tacagcaggg tttactgcta tgttgtgtgt gaacgcttta atccgtcaag gtatgacctc
    22141 cgataaaaaa gttgtcgtta ctggtgcttc tggcggtgta ggcagtacag cgatagccct
    22201 gttacataaa ctaggattcc agtcaatcat tgccttttcc agaaaagaag agtctgtcac
    22261 atggctaaaa agtctaggtg ctttgcaagt cgtacgacct gatgaatttc tgccagagac
    22321 aacaaaagct ttaggaaaac agcaaatcga ctatgtcatc gacacagtag gtggcgagca
    22381 gctaagcagt cttcttccat tgatctctta taacggtgca gtagctttat gtggcaatgc
    22441 tgggggaatc aaactaaacg cgactgtctt gccttttatt ctgcgaaaca tccagttgat
    22501 tggaattgat tctgtcaatg taccaataga tcaacgtctt tctttatggc aacagatggc
    22561 ggatttgcag attgcagatg aactggtcgt ccaagagatt actctggatc aattgcctga
    22621 aacagcatct aaactacttg ctggaacaca ccaaggaaga acactagtaa atgtaggtga
    22681 ccataaatga agattctaat cacagctgga ggaacgactg aaaaaatcga tcaagtacgt
    22741 gctattacaa atcattcgac aggacgatta gggcaagcat tagctgatta tttagctgcc
    22801 aatccggata ccacaatcga ttacgtgact acgaaacaag cactgaaacc gaaaagacac
    22861 tccaatataa cgatttatac gatcgaatct gcactggact tatttttaca attagaagaa
    22921 ctaacgaaaa aagaacacta cgatgcgatt atccacagta tggcagtcag tgattttact
    22981 cctgctttta gtttttcaga agaacagctg gcaaaaaacc tacccacttc ttcaacgcaa
    23041 gaagaactgg acaattggtt tgcggaaaat gaacagacag ataccactgt ttcaaagatc
    23101 tcttctaaca ccgagcatct tgtgctcgtt ttgaagaaaa caccaaaaat catttcttat
    23161 ctacgtgaat ggcagccaaa agcaaaaatc attggtttca aactattagt agatgtacca
    23221 aaggaatctc ttctggcagt cgcaaaaaat agtctgataa ataacaaaac tgattttatt
    23281 ttcgcaaatg atttgacgga aatccatggc gagacacacc acggctatct cttatctaaa
    23341 gatggaacag tggaagaagc tcaaagtaaa tctgagattg ctgcattgat tactgagaaa
    23401 attagactgg aggaatcaaa atgaaaaaga tcttactagg cgttactgga agtatttctg
    23461 cttacaaaag cgctgatctt acaagtcaat tcgtcaaatt aggtgcccag gtagatgtga
    23521 ttatgtctaa aagcagcaca cattttatca cgccattaac cttgcaatct ctgtcgaagc
    23581 gcgcagtcca tacagacgtc atgcaagaat caaatccgca ggtaatcaat catatcgaac
    23641 tagcaaaaca agctgacttg tttctcatcg cgcctgctac agcaaacatt atcggcaagc
    23701 tagcaaacgg cattgcagac gatcttcttt caacagtcgc aatggcgctt ccagtgggaa
    23761 caccaaaatt gattgcccct gccatgaaca cgaatatgta tcaaaacccg atcactcagc
    23821 gtaatctgga aacattgaga tctgtcggct atcaggaaat tgaaccaaga gaatctcttt
    23881 tagcttgtgg agattttgga aaaggtgcct tagctgatat tcagacggtc atcgaacagg
    23941 tcatgctgac tcttaatgaa agaaatgctt agaaaaaagg agtacttatg aaaaatacaa
    24001 aaaactttac attaactgcg atgtttttag ctattttgat tttattagct gttacgcctt
    24061 taggttttat ccctatcggt ccaatcaatg caacaaccat gcatattcct gtaatcattg
    24121 cttcgatcgt tttaggacca agactaggtg cttttcttgg cggtacattt ggactgatca
    24181 gcatgatccg tagcacagtt atccaaacac ctttgtcttt tgtcttttct ccgtttatcc
    24241 cagtcatcgg aacagaacat ggaagctgga aagccttgct gatcgcgttt attcctcgca
    24301 tcttgattgg ggtggttcct tatttcgttt ataaaggtct gcgcaaacta atgaaacaaa
    24361 aagcagattc cgtttcttta ttcttagctg gactttccgg ttcattgacc aatacgattt
    24421 tagtgatgaa tatgatctac tttttattcc agcaagatta tgccaaagtc atcggaacaa
    24481 atttaaatgc tgtttattca gctattctag cggttatttt tacaagtggt atacctgaag
    24541 gaatcgtcgc aggtcttgcg actgcagcag tatgcaatgt cttgttaaga tacttcaagc
    24601 acccttctct tactaactga tagtcatcgt agccgatctc tgattttttt ctcttttggt
    24661 gttactattg aatggaaaga tttgacgtcg aaaggatgat ccaaatgaaa atttttgttg
    24721 taggtgcaaa cggacaaatt ggccgacacc tgataaaaga tttagctcca agttcccatg
    24781 aaatttttgc aggggtgaga gatgttgctt cccaaacatt agtaaaaaaa gagaatgttt
    24841 cgtatgtctc ttttgattta acttggtccg tggaaaaaat gaccgaagca ttcaaaggca
    24901 tcgatgtatt gatttttgct gccggctctc aaggaaagaa tcttctgcaa gtagacttgg
    24961 atggcgcaat caaaacagtg atcgcagctg aaaatgcgca tgtctctcgc tatctgatgg
    25021 tcagtgctgt ttatgcagat gaaccagcaa aatggcctga gtccatgaca gattattata
    25081 ttactaaaca ttatgcagat gaatggctga aacgaactaa cttagatttt gtcattcttc
    25141 aaccagtcac attgactaac gatgaagaag ttacttccat tcaattaact aagcctaatg
    25201 agaaggcatc aaaaacgatc actcgctcta cagttgctgc tgtacttgcc gctctagtgg
    25261 aagaaacgga tatttctcgt acgactctag tactttctga aggtagcaaa gagttgaata
    25321 cagcattcca agaatgggca aaggaggaat agatccatgc gtgccatttc aatcaagcat
    25381 cctggttctg tagatgtatt agaacaaatt gaagtgcctg ttcctaaacc aaaacaaggc
    25441 gaattactga tcaaagtcca tacagcagct gtcaatcgaa cagatattat gaaaagagaa
    25501 tcagtctctt tgaaacctcc ctaccctatt ttgggagtcg aagtagctgg agaggtagtt
    25561 gagaataaaa gtgatatctc cacttttact cctgggacaa aagtatacgg attagtcaat
    25621 ctaggtggtt atgccgaata tgctgtgatg cctgccgatc gtgcaatcct tctccctgat
    25681 acgctagact atgtttctgc tgctggaatc tcagaagttt ttttgactgc ttatcaaaca
    25741 ctttactggc ttggaaaatt acaagaaggc gaaactgtct tgatccatgc aggagcaagt
    25801 ggtgtgggaa cagcagcgat ccaattagct agacagcttt ctcacgcgaa aatcatcgtt
    25861 actgcaggat cagaagaaaa actcgccttt tgtaaagaac ttggtgcaga tgaattgatc
    25921 aactataaaa aacaagactt tcaagaagag gtacgaaaaa tcactgaagg tcggggagtc
    25981 gatgttattt tggatttcat cggcgcttct tactgggaaa agaatttagc ttctatagcc
    26041 gttgatggac gttgggtctt gattggtatg ctaggtggga ccatcgtgcc tgagatagac
    26101 cttggtgtat tgattgggaa acgtgtccaa ctgcttggta cattattgac acctagaagt
    26161 gatcagtata aagcagagct cgcacaaact tttatgcaaa aagctgggcc ttttctagaa
    26221 aatggcaaga ttcggccaat cattgaccgt acattccaat tcagcgcagt caaacaagcg
    26281 catgaatata tggaagcaaa caaaaacatc ggaaaaatca ttttgaagat aactgaataa
    26341 aatcatgctg atcaaaaaag aggctgggac agaagcgttt aactccaaga aataagaaga
    26401 aattcacgaa aattgctttt caaatttttg tgaatttcag cttatttccg aaggaatttc
    26461 ttctgcttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt
    26521 cccacgctct ttttccgaat aaacggtgtt caaaagcttg catctgcggt aataagtcca
    26581 accaagttaa aaacataaga agctgttttt acttggctgt tcttattact tgatgcaggg
    26641 cagctttttc acaacctctt cactaaacag tgttcaatca gctgaccctc ggtattagct
    26701 caaaacgctg atttcacaac ctctttttat ttacgttcta tttcattcat tttccgaagc
    26761 aaatcgagct gcttgtatgc ccgcttgtcg cccaaaaata atgatctccg caactgaatt
    26821 tccaccgatc cggttgtttc catgtaagcc acctgtgact tcaccagcag catataaccc
    26881 cctaattggc tggttttctt tatctaatac ttcagtgttc gtattgattt tcacaccgcc
    26941 catcgtgtaa tggattcctg gtgcgatttt gatcgcataa taattcggag acgataagtc
    27001 atgttccatg gctgtggcac ggttgaattg agcatctttt ttctccgcaa catccgtatt
    27061 ccatccatca actgtttttg ctagtgtttc ttttggtaaa tcgatttttt ctgctaattc
    27121 ttccattgtc tttccagttg tgacatatcc tttttggtca taaaattcaa tagctgtggc
    27181 acgatcacgc acgccttggt caaaaatcaa ataagctgat ttttcaggca atgctgtaat
    27241 tgctgcagat actttatccc ttgtatccat ttcattgaca aatcttttcc cctcttgatc
    27301 gaccaagatt gctccttctc cgcgaacaac ctcaccaatc aagaccccct catcctgctg
    27361 cacagttgga tgaatttgta ttttatccat atcaacggtt tgtccaccta atttttcaat
    27421 catggtgata ccatctccag ttgatccttc ttgatttgtc gtaatataat cagctaaatc
    27481 gggacgatat ttttcaataa attctttgct ggcaccaaaa ccgcctgtgg taactacgat
    27541 tgcttttccg gtgacctctt ttggttcttc tccttgaaca tgtacagtca cttcattgac
    27601 cgtttcccca tttttcttaa tgtctgtcac atctgcgttg acaaaaatag ggatttcccg
    27661 ctcatggacg ttattcaata acccctccac taagtatcca ccaatagccg agccatcaga
    27721 cggacgatgt gtcctctttt cactcatccc tccagtgatc gttaagttgt ccagtttgat
    27781 accgttttga tctagccaat cgaccgcatc agcagagtga tcgacaaagt atcgaagcat
    27841 ttctttatca ttcgttcctc caccaccttt tagcgtttct tcatagaagg catcattcga
    27901 gtctttgatt ccttcttctt tttgaaattt tgtttctgaa gcattcatac cgcttgatga
    27961 tttagatgtg tttcctccgg caacaggcat cttttccaaa attactggat tcaacccagc
    28021 atcttttgct tcgatagctg ctgccatccc tgcaccacca gctccaataa tcacgacatc
    28081 ataagattct ttcatttctt ttggatctgt atatacttgt tcagaagcgc ctgatgatac
    28141 ttcggcagat tccttcgtac tagaacttgt ctgcgctgta tttttctgat ctgcgccgca
    28201 tgcgctcata gcaagaagtg caacggctgt taataaacca gcaactaatt ttttcatttg
    28261 tatccccctt ttctccttat actatattaa gtgatttttt tcacaatatc aactctgttt
    28321 ttctttaagt catatagctt tggtaaacac acgcttctta gtataattaa ggatatacat
    28381 gaaggagtta ttttatgaat catttgaaaa ccttttttac gaacagtaca aaaactgcag
    28441 gtagaatcac aaacggaatc tatattttat tacgtatcat cgtcgttatt atgctagtgg
    28501 atcaggtctg gaaccaacac tggggaaata caatgctttt attactgaca ttaattttat
    28561 ttgaaatccc ttattttctg gaaaagatat taaagataca gattccaaat atgctagaaa
    28621 tcattttgtt atcttttatt tttagttcaa cagttttagg agagttaagt gatttttatg
    28681 gctatttttc aatgtgggat acttttctac atgcattcag cggattttta gctgggggtg
    28741 tcggcttttc ccttgtttat ttgctcaata aaaatacaaa aaccatgaac ttgactcctt
    28801 tattactagc agttgtttcc ttctgttttt cgatgacagt tggtgttatg tgggagtttg
    28861 tggaattttc agcagataaa ttactgcaat tcgatatgca aaaagatacg atcatccagc
    28921 aaatcaatag tgtggagtta agtaaagacg gaaacactgt ctatcatgtt ccagatatca
    28981 ctcggacaat cattgaaagt caaaatgcag atggtaaaac agataaaaca atcatccaaa
    29041 atggttactt agacgtggga atcatggata ctatgaagga tttatttgtt aattttattg
    29101 gagcagttgt tttcagtgta tttggttacc tctatagtcg aaacgattac caaaaattta
    29161 cgttcatcaa gaattttatt cctaaaaaaa tcaaacgata aaacatgcta aaaaacaaga
    29221 gaaggggcag tgacaatact tttgtcactg ccccttcttt tttgaatgta tggcatgcca
    29281 ttagttatcc gatagtatta actcaaaaaa gtaagatatg aggaatgact atttcgtttt
    29341 ttatcccaat actgaggact tatttcacca tttcgttatt tatctaattt acttaaaacc
    29401 tgcataactt tttcataatc tggattaggt gtgccgtcta gattttcagc aataaaagta
    29461 aaacccattt ttttataaag agcaatcgcc tgatggctcc atgtctgtgt gtgaagatag
    29521 attggtccgt ccgtatggag ggtaggaaat tcttgcaaga ctctggcagt caatgctttt
    29581 gcgagccctt ttccttggta atctggtttc actgctaacc aatgaaaaac cggatacact
    29641 tgctctcttg atttttttcg ccaagcggta caggttgcta ctttttctcc tttttttgtc
    29701 tctacaaaaa acatccttct ttcaagttct tcaggataag gagcaaagtt ttgttgaaaa
    29761 tactcaagtg cttcatctgt cgtttgaaat tccccaacgg aagtctcaat ctccgcccaa
    29821 gcagattcat cttctttttg ataaaatcga aattcaaaac cttccgctaa aggcgtgatt
    29881 ggaacatttt gttgaagtgg tcttgtcatc cagatttctg cgtaaggtaa cgttttatct
    29941 aacatttttc tatccctcct cgtctccatt atgccaaata acatatgact cagctactta
    30001 tttgactata acagatagag atataaatca gaaccagtca tctgcgtcaa attcatttcg
    30061 attagcgtat acccatgttc ctgccatacc tcgcatgaaa tctcctaaag cagaagaagg
    30121 aatacctcca ccaggtgcgg gtgttcccgg actaggaagt ggtacttgcg gctgcagatg
    30181 ctcatcggtg gaatcaatag ttgacaagcg ttttttcttc gtttccttct ggtcatctga
    30241 ttccttctga tcttccgact gtttagcaac aaattctttt tctgattgtt gttttatttt
    30301 aggctctaaa atttcatttt ccaatccaga aacttgtggg gccaaatgag cttcttgcct
    30361 tatcgtttta ttagattctg agatttttct agatattctt gctaactcta ggcttcttgt
    30421 ttgttttact ttccctgttg gagaaactgc atgagccatt tgttgttttt tttctgacaa
    30481 gctttcttct tgtctttttt gttctttttg atctatctgt ttgtttaatg gtaaaacatg
    30541 taatagtgac tgagcatgga gttcttggag tgaatacggg tcatgataac tatacataat
    30601 cgtaaaatca cctgtttgtt tggtcaaata accatcctcc aatggtactt cattgattgt
    30661 aaccgataca tcctttaacg gtacgggttt acctaaggaa tttgttgctg attggatcaa
    30721 ctcttctttg tttatttttt cgtttggatg aacaataaga tcatgacctt ctaatgaacg
    30781 attgtctggt atataaacat aagtaaaggt aatttcccct cctgattgct tcctattatt
    30841 catcagttct gagtgaagta cttcaaggat ctccgaccag tgactttttc tgtctaatcc
    30901 gtttgcttgc atccaatcag aaatagaagt gactttccca ttttgttcat aagtcgataa
    30961 atctatattt ggcactcgat cagaaaattc agcaggtagg cgcaaatcag gaatacgatt
    31021 tacactactc atatgtgttt ctgcttgaag ttctttattt gtttgactat ctataaaatg
    31081 agtgatgaca gtcgatgaag cagcagatgc ttctacataa actacagctg ttgtttctgc
    31141 cacatttgat gttttcgagt ctatataagt taatacaacg ggatattctc ccggagttgt
    31201 agtatctact gtcccgttca ttttgatttc ttctttattt cctgaagttc catcgctttt
    31261 tgttacatat tcaacaagat ctttaggggc gtaactctct cctgttctta agtgaagatc
    31321 ttttcctttg atagctgttt gatcagcacg aacatggatc acagcagatg cagtcattgt
    31381 ttttaaagca attggatcaa aataggacaa ggaaatagta gatgttcctg tattagtcgt
    31441 atccacgata ttcccattgt ttgaaattac tttggacata tctccttgtg ttccatctgc
    31501 gttcttcagc ctttctacca gatcagatat cgaaactttt gtccctgcca gaacatcgat
    31561 tttctttctt tcaaactgtg aataatcttt ttcatataca taagaaactt gtgcgcttcc
    31621 accgtccttt atatctgtca aagcatgatt caaaatatca gtgatctgtt ttaactgatc
    31681 accttttgta ggatcagtca tatcggaaaa gatagaaagc ggcacactgc ttccggtttc
    31741 cgatgtccat aatgttttgt taggaattaa ccagtagtgg tttatcgttg gaggataact
    31801 gactttatta taggaagtca atgcaggaag tagctgtttt ccatttgcat cagtgaaagt
    31861 atgagtaata gtgatagggg aatcagtggt ctcagaaaca gaggtacgta cgaccttgat
    31921 ggtttgtgtc gttgcctgaa cgggtacatg catatagaca cctacagata aactgatcag
    31981 catcgtgcgc atcataaact taacgtagtg tttttcagaa cacgaccgtt ccttatctaa
    32041 acgacaagcg tgtttattta gttgttttga acgtcttttt gaacacattt attcttagtc
    32101 ctccctactc ttgtctaaac atgaagtagt aattttcata agatgatttc attatataaa
    32161 taaaatcaaa agaagatgga ctattaggag agtatttttt ttgatttttt attgtttcaa
    32221 gaaaatcgga ataataactt atgtactttt taattttgtt aagtaatata tctagttata
    32281 tctagttata tctagttacc ttttctttga aaaaaacata aatcagctaa taaatatgtg
    32341 agacaaagct gccatccata aatactagct gattttttca gttaaatgtc tatttttttc
    32401 tctgatgatg gtatttccaa aatattccca gtcatctgga gtcggtggat ttttccaaat
    32461 tatctgttct ttattaaatt tttcaagtaa acaatcagat aaataaatat tcgtacgaga
    32521 agtgattcga ctctcaatca caatattgta atttttaaat cctttcactt gagaaataat
    32581 gtaatcggta taagcagatc cttgcgaaaa atcaatacag acatgaatcg gtgcttcaaa
    32641 atatgatacc ggacaaatac cgaccaataa aaagagataa tcataaaata agcgaatcat
    32701 gaaacgttca tcttttccca agaaatagcg tttattctct tgtatgacct tttggattgc
    32761 tgtactgaat attggatacg tctcgaagaa aaactgtagc tgctttttag acgtaaagct
    32821 agtcatctca aagtcgaata tccaattttt tcgattgaca tgcatcatac ccatacgaag
    32881 ttcttttcgt atatatagtc tttcttcttc agagatttca atcgaaaatg gtaattgatc
    32941 aatcaattcc actgacattt tttgactaat catgtggata tcatgaaaat gagcttccgc
    33001 aagtccaaat gcgccatcta attcaaattc ttcttccgtg atcaaaaaca acatgatata
    33061 agaaatctca tcaatgatct gctctttagg gatatgaaga aaagaaatat gtgataaatc
    33121 cttgatgatc ttcattcctt ctgagctctt aaagtattca tctgaaaaga aacgttttcc
    33181 gattggttga ttcccatagg ccctcaacaa actaatagtg gttagcaaat tgaatttgat
    33241 tcgttttgtt gcagacagtt ttaaattgaa cagatattca aaatattgca cgatactctt
    33301 tgcttcattc atgacgcttt ctctaaatgg catagaaaaa ccattaaaga ccccgaaata
    33361 taccccaaat aatacgttcc gtatgtttat ttcttcccca actaatatgt ttttctttaa
    33421 ctttatttga tgctgttcaa gaaaagagac taattctctt ctttttacat aggctcttga
    33481 tatgcttaaa aaatgattct ctgcaaattt ttcgatcgtt acttttgaat ccacgatctc
    33541 cgttaacagt cgagcaattt ttgatcgttg aaagtagatt cttcttaaat ttttgatatc
    33601 cgtcaaattc agacgttcga catggatctc tgcttcttct gaaatttgga tctctggctt
    33661 tgttttgaat tcagtaaatt ctttatttaa ttctatgata taatttcttg ttttaaattt
    33721 agacaaacct aatgtttcat ataacttagg cagcactaca taattctttt tctcaaattc
    33781 taaatatcta gctagcagca gtttttctct atcttcctct tctataaggt aatccaactt
    33841 catatcctac acctcaaaaa atgcctcata ccaattactt gatatgcaga tgaaagttca
    33901 tcttttcacc tatactgatt ttatgatgga ttatgcttgt tttttcatca gttattctca
    33961 ttttttatct tttttttgag aatattgcaa aaaatggaaa aatttatgag gaaatattct
    34021 atattttttg ttttattgtg ttaaaatgta caagatttgc gccatttgcg taaacaacca
    34081 caaaaaagag caggacggcc accgtcctgc tcttttactt attttaagtt ccattactta
    34141 ttaattggct aaacttctgc ttgtttctga tattgcttgt ttttctccaa tgatccgcac
    34201 ttctgtttct aaagacacat caaatttttc tttgattact tcttggatgt gcgcaatcaa
    34261 ttcaatataa tctgtcgctg tcgcatgatc gatattgacg atgaatccag catgtttttc
    34321 agagacttgt gcaccgcccc atttcaggcc ttgcaatcct gcttgttgga tcagctgacc
    34381 tgtatagtgt cctactgggc gcttaaagac acttccgcat gaagggtatt ctaatggctg
    34441 ttttgattga cgcaattcgg tcaactcatc cattcttgct ttgattgctt cataatcccc
    34501 tgtttgcaaa ctgaatcgag cttccaatac gatagcacgt aattcttgga ctttactgtg
    34561 acgataagaa aataccattt cgtcttttgt caaagttttc aatgttccat cttcaagcaa
    34621 aagatccact tcagcaaaaa catctttgat ctctccgcca tacgcacctg cattcatata
    34681 aaccgctcca ccgacacttc ccgggatgcc gcaagcaaat tcaaatccag tcaatgattc
    34741 tgccaaagct gcataagtcg tatcaatcag tgaggcacct gcttccgcaa tgaccatcgt
    34801 tcctttgaca tgaatctgtt tcatttcagt cagcatgatg actacaccgc gaatcccgcc
    34861 atcttgaacg atcagattgc ttgcatttcc taataccatc caaggtatat catggattcg
    34921 gcaataatcg atgatttgct ttacttcttc ttgttttttc gggaatgcaa ggacatcagc
    34981 tggtccacct gttttcgtaa acgtatagtt catcaatggt tcatcaaaca atatagtaat
    35041 cgtggctaag ctttcaacta tttcttcttt attcactgag gattttcctt tcttttctca
    35101 aacgtcctca ttattctatc acgcttaata caatcttgcc agtcttgttc aaagttgatg
    35161 acggtagtga tctttaagca cttcctcaca taattcatga cttcttacaa cttctttttg
    35221 tttaagcaat tctgtttccg atttacctaa acggatctga gaaaagaagg cctctaccat
    35281 tggctcaaac ccgcgtttga ctagcgtgtt tgtccagtcg ccaaattctt tcactttttt
    35341 tccatcagca tcttgaatga caagctgggt cagatcgttt acttcataag taccattttt
    35401 acttgttact tgatattttt ctatattagc gccacttgct aaatccatcg ttaaaatagc
    35461 cgtttgattt tgtgtttcta attgtaaaat ggcatattcc aaaaattcct ttgtttctct
    35521 tatctggtat tttgttctca aaatgggttc atctaataaa tacacagctg tatcaataag
    35581 atgtaaaaac aaatcataaa ggacgaactc tgtcgtttct tttgccgcta ttcgattttt
    35641 ttgcaggtgg atcaaccgct tatctgggat acgtttcaat tcttctacca taggagcaaa
    35701 gcgccggttg aaaccgatca tcaaaattat ttggttctta tcagctaatt cttgcaattc
    35761 acgaacttca tttagatgga cactcagtgg tttgtcaata taaacatgga taccattttc
    35821 tagacatttt ttggctaatt cataatgtac ttttgttgct gcgtggatca tacatgcttc
    35881 gattttttct tctaaaagct catcgatcgt ttcatataga tgttggaacc cgtactcttt
    35941 ttgaattctt tcttttgttt ctttatttct tgttgcaaaa taaaaatcaa gtgttccttg
    36001 ttttttcgca taagttggta aatatgcttt ttctgcgata ttccctacac cgattacacc
    36061 aatcttcatc tactttcctc ctggatcatt tagttacttt catcttacac cattttttac
    36121 taaaatgagg tacaatagta tcgcaaaggt aaggaaatta attcgaagga gtgtgctttt
    36181 attgaaattt atctcttgga acgtaaacgg gcttcgtgcc atcgtaaata aaaatttttt
    36241 agaagttttt catgagttag acgccgattt tttctgtctg caggaaacga aactccaagc
    36301 aggacaaatt gatttagatc ttccaggtta ttatcaatat tggaattacg cggaacgaaa
    36361 aggctattcc ggcacagcga tttttgctaa aaaaccagca ttgaatgcca cttacggaat
    36421 gggtatcgat atccatgata cagaaggccg tctgattact ttggaatatt ccgatttctt
    36481 tcttgtaacc tgttacacgc caaattctca aagcgaatta aaacgtttag attaccgatt
    36541 agaatgggaa gaagcctttt ataattattt ggaaaatcta aaaaaacaaa aaccagtcat
    36601 cgtgtgtggg gatctaaatg tcgctcatca aaagattgat ttgaaaaact ggaaaacgaa
    36661 tcaaaaaaat gctggattca ctccagaaga aagagcggct ctttctcgac tgttggataa
    36721 cgggtttatc gatacattcc gttattttta tccaacccag gaaggtgtct actcttggtg
    36781 gaactaccga ttcaattcaa ggaaaaacaa tgctggttgg cggatcgatt actttttgac
    36841 gagcaaagat ttagagccag gactagctga tgcaaagatc catacggaca ttatgggcag
    36901 tgatcattgt ccagtagaat tagatttgaa ataaagaggt tgtgaaaaag tcgtttagtt
    36961 tcgagtatta agaaaaattt tgtgaaaatg gctttccaca ttttgcataa atttggcata
    37021 ataccgagaa actggctttt gaacactgtt tatcgagaga ttgtgaaaaa gctgtcctgc
    37081 atcaagtaat aagaacagcc aaacaaaaat agttcctcat atttttcgtt tggttgggct
    37141 tattaccgca gatacaagct tttgaacacc gtttattaag aggttgtgaa aaagctgcac
    37201 tgcatcaagt aataagaaca gccaaacaaa aatagttcct catatttttc gtttggttgg
    37261 gcttattacc gcagatgcaa gcttttgaac accgtttatt aagaggttgt gaaaaagctg
    37321 cactgcatca agtaataaga acagccaaac aaaaatagtt cctcatattt ttcgtttggt
    37381 tggacttatt accgcagatg caagcttttg aacaccgttt attgagaagt tgtgaaatca
    37441 gcgtcctgac ctgagtatta gagaaacaaa tggcaaaata gttcctcata ttttgctatt
    37501 ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tagtggagaa gttgtgaaaa
    37561 agtcgcctac tttcgagtcg taagaaattt ttttgaaaat ggttcctcac aactttgcat
    37621 aaatttgact taataccgag aagtcatgat ccccatctaa ctccaaatca caaggaatga
    37681 gttcaaaaac tagcttttct caacaaatat tacataaaaa attctatgtt ttttgttaaa
    37741 tttatttgct tttttcaaaa gtatgttaca gtaatttccc aaggaggttt tacagaaatg
    37801 aattttgttg ccatggattt tgaaactgcc aatcatcagc cttatagtgc ttgttctctt
    37861 gcgctagtca tggtaaaaaa tagccagatc gttgatgaat tctatacatt gatacagcct
    37921 gagactcctt ttttctggcg aaatgtccaa attcacggaa tccatcaaga agacgtacgc
    37981 aatgcgccga aattcccaga agtctggcaa actatccaac catattttca aaaaaatcgt
    38041 ttagtcgttg cacataatgc tgcttttgat acaaaggtct tagcgggatg cttagattat
    38101 tatcatcttg aacagcctaa ttatctttct ttatgtacag tgaaaagtag ccgtcgcctg
    38161 tttcctgaat ttccaaatca tcgattgaac actgtgtgcg aaaatttgaa catccaatta
    38221 gatcatcacc atgatgcatt ggaagatagt cgtgcatgtg cggaaatcct gctttatcaa
    38281 gaaaaaaaat tcgggacaga tccattgaaa aaacttgtga ctataaagta aaagaatact
    38341 tatgaaacac acaaagaggt cgtgagcctg ccgtttcact ccgagtcaca aggagcaatt
    38401 tcaaaaaata gcttctcatg tttattaaat attgtgactg gtgaccgagg agctggcagg
    38461 agaacaccgt ttattggaga ggttgtgaaa aagctgttct gcatcaagta ataagaacag
    38521 ccaaacaaaa atagcttctc atatttttca tttggttgga cttattaccg cagatgcaag
    38581 cttttggaca ccgtttacca agaggttgtg aaatcagcgt tttgacctga gtattagaga
    38641 aacaaacggc aaaatagttc ctcatatttt gccgtttttt gagctaatac cgaaggtcag
    38701 ctgattgaac accgtttatt caaaaaaaat ggactgcgac acgacttttg tcacagtccc
    38761 ttcgtgtgtt tttctgcctt ctcaaaagga tgacagtttc tcactctttt cctatttctt
    38821 ctattcaatc aaataactca ttttatatac atctaaaaat tcacctagat ctgttcttgc
    38881 ccctctaggc atgaccgctt cgatttggaa gcccattttt cggtaaaggt gtacggctct
    38941 ttcattacga acttgaacgt caagctccaa tcgaaaaagt acacccattt ccttcgccca
    39001 actgatgatt tcttctagca taagagtgcc tagtcccatt ccccaatatt cctgcagaat
    39061 actgataccg acttctccca cgtgagacaa acgaaattga ctgtctgcct tgacagatgc
    39121 tgttccaata atcgtccctt catagattgc aagcagcaat aaattattat tgctttcgta
    39181 tatataatcc aactcttctt ctaaggtttc tggtggtaac aggagttctg cttcatccaa
    39241 aactaaaaat tctgtctcct tattaacttg tcccatcatc aaaagaatgt cttttgcatc
    39301 tcctggcact gcttcccgca agatgatccc aacttcttca ctcatgattt tcagcccatt
    39361 tttctagaat tcgctgtttg aaggtttcac tttgctcccc gtatgagatt agtttcacat
    39421 accgcttttc taaatctgta tcactttttt ctaaaatcag ttccaaataa tcctccggca
    39481 gtgcttcttc taatagattt ccccattcaa caacggaaag cccatcacct tcaaaatatt
    39541 catctaaccc taaatctgct ccgctagctg ctacccgata aatatccata tgatacaacg
    39601 gaatccgacc ttgactatat tctctaataa tcgtgtatgt cggacttttg atcatttgcg
    39661 ttatccctaa tccttgggca attcctttcg tcaacgttgt tttacccgca cctagatcac
    39721 cagtcaatat cagattgtca cctggcattg cggaatgacc aattgcctct gcgaattgtt
    39781 ctgtcattgt taatcctgat agttcgatca tttttcatct tctcccgctc ttctttcttt
    39841 ttgccagtat atcgcgtaat gaagtttttt taaagaaaaa atgaactagt caagaacagc
    39901 agctgctgtt tttggctagt tcgcgtgtat ttgttaatca atcaatgttt gtgctgctgt
    39961 gataatcgat aatttatata catcttcttc atttgctcca cgagacaaat cagaaaccgg
    40021 tttgttcaag ccttgtagaa ttggtccgat tgcttcaaag ttaccgaaac gctgagcaat
    40081 tttatagccg atatttcctg actgcaattc tggaaataca aagactgttg cttgaccagc
    40141 tacttctgaa tctggcgctt tcagttgtgc aactgaagga acataagaag catcaaattg
    40201 caattcgccg tcgatcatgt attgtggcgc tagtttttta gcgattttag ttgcttcaac
    40261 tactttgtct acttctggcg atttcgctga gccttttgtc gagaagctca tcattgctac
    40321 ttttggttcg atatcgaaca attctgctgt ttttgcagaa tctacggcga tttcagccaa
    40381 ttcttgcgca gttggattca cgttgatcgc acaatccgaa aagacatatt tttcttgatc
    40441 tcttccacgt accatgatca tcgctccgct tgtccgactg atgccaggct ttgttttgat
    40501 gatctgaagt gctgggcgaa cagtgtcgcc agtcgaatgg atcgctccag agaccattcc
    40561 gtctgcaatc cccatatacg taagcatcgt accaaagtaa ttcacatctt ttaaaatctt
    40621 ttctgcttgt tctttggatg cctttccatt tctacgttca acaaaagtat ccaccatttc
    40681 atcccactta tcgtaattat cagggctgat gatcgtaaag ctagatgttt tgattcctct
    40741 ggcatgcgct gcatttctta tttcttcttc gttccctact aaaatgggtt ccacaagttc
    40801 ttctgctttc aaccgggcag ctgcgcctaa aatacgagca tcagtagctt ccgggaaaac
    40861 gattttgatc ccacggcgaa caattttaaa tcttaaacta tcaaacaatt ccacaagatg
    40921 acctccagac gtttttatat gtattatcat acactattcc agaaaaaaag aacagtttat
    40981 tttatctgta tcagtcaaaa atgctggaaa aattaagttt ttcacataca aagcgaactg
    41041 cactataaca ggaaagcgct gttttattac actgtttcag gcagttgcca atcaatcggt
    41101 tcttctccta acgcaaggag cgcatcattc gttttcgaga agggtttgga accaaaaaag
    41161 ccacgacttg cagataacgg actgggatgt ggagatgtta tgatgatatg gcgactctta
    41221 tcgatcattt tgattttttc ttgtgccggt tttccccaca agataaacac gattggtttt
    41281 tcccgttcat ttaatttttc aatgatcgtg tctgttaatc tttcccaacc tttcccgcga
    41341 tgagaatacg cttgcccttc ccgaacggta agaacggtgt tgagtaaaag gactccctgt
    41401 ttagcccatg acactagatt tccatgttga acaggtgcga ttccaagatc atcataaagc
    41461 tctttataga tatttcttaa cgatggcggg atctttacgc caggttgcac agaaaaagat
    41521 aagccatgag cttgattttc tccatgataa ggatcttgtc ctaaaatcac cactttaact
    41581 tcttcataag gagtcaattc caatgcttca tagatatgat acatatctgg atggatcttt
    41641 tgggacgcat attctttttt taaaaattct cttagatgca gataataatc ttttgaaaat
    41701 tctgaggaca aaacttcttg ccaactgtta tgaatgattt ctttcaaaaa ctccacctcc
    41761 taataaaaag aataactcac attttcttca atcacaaggg acaatcttca ccctcagcac
    41821 ttgattaata tggtaaacta ggaacaagaa gcagaaagga acgataatca ttatgatcaa
    41881 attaattgca tcagatatgg acggcacatt attagatgcc catatgagtg tttctcctga
    41941 aaatgccgag gcgatacgtt ttgcaaatga tgccggtgtt gagttcatgg tcgctactgg
    42001 tcgaaactat caagaagctc gcgcagcttt agatgaggta ggaatcgatt gtgcgatgat
    42061 cactttgaac ggggcacagg tatttgataa agaaggaaat tcattgttta ccgtgtcgat
    42121 tccaaataat caagcaatct ctgttttaga tattctagat gctaacggta tttactatga
    42181 agtagcaacc aacgatggtc tttattctga aaatcaagcg aaacgaattg aaagttttgc
    42241 ttccatggtg gctacccatt tacctcacct tacgtataaa atggcgattg ccatggcctc
    42301 agctaagctg gagttattgc acatcacata cgtggacagt attcgtgcta ttttggaaga
    42361 aaagaatctg gaagttttaa aaattatttg ttttcataca gaagggccag ctgtattagg
    42421 tccagttgga aagcagattt ctcttttagg cgatctagcc gttacttcct ctggtcaaaa
    42481 caatatcgaa gtgaaccata aaaacgctca aaaaggaatt gctgttgccc atgtcgctca
    42541 cgagcgtggg atttctttag aagaagtcat gacgattggc gataacttca atgatgtcag
    42601 catgctgcaa gtagcaggag tcagtttcgc aatgggaaat gcagaaatcg aagtaaaaga
    42661 ttatgccaaa tatacaacgg acacaaacct tgaatctggc gtaggaaaag ccatattgcg
    42721 cgcgattgca gaagacttgt agttttatct agaaaagagg gatatagatg tcagaatttt
    42781 tcattcagga aaagcagctt ggacgtgcaa ctcggacaat tgtcaaaaac gaaaaaggac
    42841 attccctgtt tttgatggtt ggtcgctggg gaacaaaggg cgatgcattg tctctttatg
    42901 ccatgaatgg cgatctcgtt gctcacatca agcagataag cctgacattc ggtacgcgtt
    42961 ttgagctgta taaagagttc gaaaaggtgg gaaccatgca gaaaatattc aattggcccg
    43021 gtgactttta ttatattcgt agacttcact ggacagtcca aggtgatatc tataatcatc
    43081 aatatcaaat ccatcatttc aatcagcaaa tcatgaaaat ggataaagca acactttttt
    43141 caggagatta ctatgtattg gatgtctcaa atgatgaaga tgctccagtt tgtatttgta
    43201 ttgctgcggt tttagattac tggctctacc gtaaagacaa aagtcgcgat aagcctgttt
    43261 cttaccgact taacttaagt taaaaagatg tacctgaaat gcggaaatac gcatttcagg
    43321 tacatctttt tagatattct tttgcatatg atcataaaga tgaccactga tcatccgctg
    43381 accgacaact ttgaagtcat ggcgttcgta taatttttta gcctttggat ttttttcatc
    43441 cacacttaga ccgatgacgg agcgattagc tttcttcgcc aatttaggta atgcttctaa
    43501 taatcgcgaa ccgatgcctt gtcctctaaa atcttctgat acactgatag agtccaaata
    43561 ccattcgttg ggaaacgttt ctttatcaat gaacaaacgt gtagacgcat ctagctgata
    43621 tttctctaag ataggtacca gtggctgatc gatgattgat tcttcttcat ctgtataacc
    43681 gtaagcaact cctgcaactt taccgtctat ttcttctacg atacctcgtg aataactgta
    43741 acgataattt ggatcagtga ttgcttcttc taaaatctct aaagtctttt gttcccctgt
    43801 ctgcttcaaa aatggcagtt ccatatcttt tagaatcact aagatcaatg gggcgatttc
    43861 tggagcatct tcttttctcg caaaacgaat catagtttcc tctccttttc tccattctaa
    43921 catgcagaaa aagaaatcgc aaaatcgtta ttttaaaaaa tcttcaatct tttcagtaat
    43981 gaaataacca tgagtaacga ttcttgccgc ttcagttcgg caataagggc agacgactgg
    44041 catttcttta tcattatgtt cgactacatt attgacttct tcgtatgcaa atttttttcc
    44101 gcaattaggg catttcattt tttccatgtt atctcaactt cctttcatct ttactctctt
    44161 agcttactct tatattgtaa aaaagagaag caatatcctt cttctatgca gatatccata
    44221 cctgacggtc ctgttctcca tctattaatc ggactttata ataaggacgt tcatctttga
    44281 ctagaagtgt ccactctttc acttttgctt ttttcccgta gtagctgact gcttccttca
    44341 tcgctgcttg cggttgtttg aactcttccg ttttttctat atcaaaaaga tgttctgcgg
    44401 ccttttccgt gtcatttcgt acggttgttt tcccagtgga tggattgact gctatgactt
    44461 gtccttcaca aaaaaactgg tattcatatg ttgcattcgt aggattctcc aaatcttttt
    44521 caactgcaaa atgaatagca gatatgttat gttcttttgt ttcgtttttt acgattgctg
    44581 ctgctttttt caatgagatc cgatattcct ggtcactaat atcatccatg tgaaaagcgg
    44641 cattgagcag tcccgtcata ccggtaatcc ctattacgat gagggcagca acaccaaaca
    44701 tcattttttt cattcttcat tctcctgttt ctttttttta aagaatatca agaaatgaac
    44761 gaatgaaaaa tcgaatgaaa cgaaaaaaaa gtgacaattt tgttatcttt attcgtgcag
    44821 ttctaatggc aatccgtctg gatcaaagaa aaacgtcatt ttttttcctg taaaggtatc
    44881 ttctctaatc ggttctgatt cgatccctaa ggactgcaat tctgcaattg tttcttcaat
    44941 atcgtctact ttgaaagcaa gatgacgcaa gccacaagct tctggatagt ttggtcgttg
    45001 tggcgcatta gcaattccaa aaatctccaa ttcagcactt cctagtttca gatctaattt
    45061 atagtcatct cgttctttgc ggtaattttc tctgattact tcaaatccta atttattaac
    45121 ataaaaatca cgtgatttct gataatctga aacaatgatg gctatatgat ggatcgttgt
    45181 aaggttcata tgagcactcc tattcttcgt ttaagctcgg atctatatta atctccagtt
    45241 ccattgtaaa tgtgtcagat tgaaaaaaat agctgttttt cctttttata agaaaaaagg
    45301 caaaaaaaac agaaagtagg tcctccaaga cactcttcct gttcccaata taataaatga
    45361 aagtaagaaa ttatattttc aagaaaacaa acagaaaatt atcataacac aaattattta
    45421 taattgaaag actagttttt tttcaagaaa agcatacaat agattgtgtt agtaaataca
    45481 gaacacacta catttagtgt cttaaggagt cctcatacat gaagtcatat tttcagtttt
    45541 tagctcatca gctaaaaggc tggccgcaac aaaattatta tcttttcttt ttcagtcttg
    45601 gctgccaggt catgactctt gtcaatagtc ccatcacttg gctgtccgtt attacattta
    45661 tcggtaccac gcttggtgta ttgtgtattt tatccatcaa tgcggcaaaa tcagtcaacg
    45721 gtattttagg tattttgtct gcgatttgtt ttattatcgt tgggttttca gcaaagaact
    45781 atttaagtat cggtgaacag attgcttata tgatcacatt ggatatcccc gtattgttaa
    45841 gtaaagaatg gaatcataat atggcttcaa aaatccgaaa attcactgga aaaagctggt
    45901 ttatcgcaat catcagtact ttccttgtct atcttgtttc aggattcctg attggtcgtt
    45961 atacagatga tcctcggccg tgggtagatg ccattgcttt ttctattagc ttgactgctg
    46021 gtatcatcag tttcttgcgt tacaacaacc aatattactg gtggttagct tcagggcttg
    46081 ctcaaatggt cctttggtat atttcattta cgcatggatc tgcaagtttg gcgatgttcg
    46141 tgaacagctc tgtttacttg ctgaatgatg tccttgcatt taccgtttca ccttggtata
    46201 accaaaaaga acgtgaacgt atgcaaaaaa tggaagaaga atactacaac gcacaacaac
    46261 acgcataaaa aaactgagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
    46321 cacgtataaa cggcggaatc agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
    46381 tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc ttctgtccca
    46441 gcctctattt tttatagtaa ttccttcaga tactttcttg cctcatcatt gaaacgcagt
    46501 gattcttcta acttctgata atattccaac gcttgtttct gagcttcagt aaaacgataa
    46561 tgttttcctt tccatttgtc gattgtctct tgcacatttc cagattggat atctttcact
    46621 actagttgcc atttttcttt tgagactgta cggtctttct tatcagcaga ttgtatagct
    46681 tctcgttctt tacgcaatac ttttaaaaaa ccatttctga ctatccgagg aatgctgtcg
    46741 aagaaccctt ctgtaaaacc atagcgacaa tattgcttcc aaataaatag atagattttt
    46801 ggttggatga atcgttggtt tttcgcaaaa tattccatct gagcagtgac ttctggtgtt
    46861 cgttgacgtt ttgtctttct caacacggaa ataaatgttt caaaataagc attaggatgt
    46921 actggcactt cttcatatac gatggattgc caatctttac gtaaaaatgc gcgatagttg
    46981 atcaagaatt cttctacatc ctttactaat tcttctgcaa agtcgtcaaa tagttctttt
    47041 tttatctgtc gcttttttga ctgcccattg atgtgttcac ttattttctt gatttcattt
    47101 tccaatgctt gataatccgc tatgtaaatc ggcatttcta cttggcgaaa ttctgcgagc
    47161 cgctgagcta attttatatc cggtaaatat ggctgtttca tccgacgatt ttggtacaag
    47221 acaaaacagt atttttgcca tagtccttca ggaaaatcga tattcttctg cttaagccat
    47281 aataattcgt ctaaggccag atcaactttt gtcattccct gatggatcga agctgccact
    47341 gtcggagaga catgcaggtg atcggcaaag tggttgatgc ctagttttag gatctttccg
    47401 gtttttggcg atttaacgat gtattgcact tttaattctc taccacactc acaatacaat
    47461 ttttgctggc ttcgttttgt tcgaaaaaac ggattgatct tgaagtcaga aaacacccaa
    47521 tctgttgcag ctaaataatg ttcttccata aaacgagagc tgattagata tcggcggtgc
    47581 ttatcgacta gttcccgctg ttttttcgtt aattttgcat agatctccca acgttgactg
    47641 cttgtgtatt gtttagaaat tttgatggga tagcttttgc tgatttcttt tggaaaccat
    47701 tcttcaaaga cggattgcca ttcttccacg gctgtttctt tcctttctaa ttacaaaaag
    47761 ctggctacta acataacgtt agcaaccagc cagtgtttct tgctaaatta gtgagttgtt
    47821 ccggcatttc cggaaatatc ttctttattt tctactgctg cttcaactgc ataagtcaaa
    47881 gaagaagcaa tgtcttctaa agacatagaa ggcgtaccca ctggacgatc gatcacttga
    47941 tcgggtgaga atgacacgtg gatgaaccca cctcgaattg ccgggaattg tgtttcgatc
    48001 aaatgaagca aacgatacat gatatcattg cagacaaatg ttcctgctgt ataagagatg
    48061 tacgctggta gaccatgatc ttgtacattt tttgtcattg ctttgattgg caatgaggta
    48121 aagtaagcgg ctggaccgtt ttcttctaaa gccgtatcga atggctgatt tccttcatta
    48181 tcagggattc ttgcttctgc tagattgatt gcgacacgtt cgaacgagac tgctgaccgc
    48241 ccacctgctt gtccgacaca gatcacgaca tctggttggt gctgttggat ggcttctttg
    48301 actacttgac tgcttttttc gaatacggtc ggtacttcca ctttgatgat ttcagcaccg
    48361 ctgatttcat ctggcatttt ttttactgct tcataggctg gattcacttt gtccccacca
    48421 aatggatcaa aacctgttac tagtactttc atctgttgtt cctccgtccg attgatttaa
    48481 catatggtta tcttttatca taccattttc cttgaacagc gtaaataaag gaaacaatag
    48541 tttaaataac ataacagatc aaacttaata tattcttata aaaaaatagt gttccactac
    48601 aatgttagaa gaggttgcga caattattct gtcacaaccc ctctcttcta ttatttaaat
    48661 agaatcatat aagcaatttg gaaaactagc ataaataacg caataaacaa ctggtttttg
    48721 atcactccgt atttgtcttt catctccagc atcgctacag gcacgatatt gaagttagca
    48781 gccatcggtg tcagcagggt cccacaaaaa ccgcaagtca atgcaaccat tcctattaac
    48841 gctggattcg ctccatgact aaatacgaat ggtgcaccga tcccaaccgt catcacggtg
    48901 atggccgcaa atgcattccc catgattacc gtaaatatca ccatgccaag agcgtaaatg
    48961 atgatgccga tgttcacatt tccttctgga acgatcgtac ctactgcatt ggaaatcact
    49021 gtcccaactc cagcgcttgt aaataccgca cctagagaag ctaaaagcat tggcaacatg
    49081 cttaacggcc ctactgtgcc cagcatatct gctgcatcat ctaaaaatgt acttggtcga
    49141 ttatctcgag aaaagaacat caagattagc atggctgcaa aaacaccgac gcccacgccg
    49201 actaaagcac ctaaattggt aaatagagca aacaagatcg caaagacacc gatactcaaa
    49261 gctggaataa aaattttcat tcctagtttg tctgacattt tttccatata ttcttttgat
    49321 ggaagccggc tttctccttt gctgactttc ttagcaattg ctggtatggt catcgcaaag
    49381 atcaagccac cgttcacgat tgccggaagc catcttcctc ctgcaataac tacccctaag
    49441 accgtccaga aaaaggctga cccataacga tgaggattgg ttttatctgt taaatttctt
    49501 actccagtgt aaatcaatac gagtcccatc acgatataaa ggatctccag taatttatcg
    49561 cctaataaaa tatcaggatt tgtaaaaaag tccatcttat tgcccctttc ctggataata
    49621 tttcttcgct aatttctttt ctttaagata gaagtagaca cttgccacaa ctaaagccgt
    49681 gatagccact ggaacttcca ctaaagcaag ctgtcccaat gttacgtcat atcctagatc
    49741 tttcagtgtt gattggacta gcaacgcccc tgctcctccg atgaatagta cttggccaaa
    49801 gaaccagcag acattttcca tagctgaagc catacctttt agttcttctt catgcttagg
    49861 atggacttct aacccttttg attcgatggt ccccaatgcc atcggtaaaa tgattggacg
    49921 aacaaatcca gcaactccac caaaactcac gttaaaagcc gcaaagatca ttcgaaagat
    49981 cccataaata tctacaatca taccagcaga tactttcttg aatcgcttga tcaaggaagc
    50041 cgctgattct ttcagaccat ttctttcaag cgttcctgtt gccaacataa tgatgataaa
    50101 aatcgccatc ccgcgattgt ttacaaaact tgtacctaaa agttctaaca ttccttgaat
    50161 gccaagaccg ccagtcaaag ctgtaacaac taaagcaacc attacgatca atatcgagtc
    50221 taatttaaat gcaaacccga gaataacaat caagacgcct aaaaggctaa gataattttc
    50281 catatatttt cctccataaa atgacaatat ttttttaatt ataaagcaaa acacataaaa
    50341 aatatagttg ttttctcacg aaatagacac tatttttaaa tatttttcgt tattttttta
    50401 tcacaaaaat tatcttactt attgtaaggt aaagcttttt ttaatttatt gcccgataat
    50461 atatagaaat aaggtctagt tactgttttt tttaaatgat taataaataa caacttcttt
    50521 ctatgaaatt gcaaagcatt catcttcttt tccgctaacg atatatccgt cagtgcttgg
    50581 tacactttac taaaaagtat gagattgccg atatctggat aggtgtgcta tactacagaa
    50641 aaagatatac aatctgccac cgggtaggat aaagaggatg atcattccat taggtctttg
    50701 aagggatgat tttcctcttt ttgtgtattt ttttatagtt aaggaggaat gtaagtatgg
    50761 catggatgga attaatcata gcaggaattt tggaagtatt ctggtcaacg atgatgaaat
    50821 ggagtgatgg tttttctaaa atcaattatt ctttttacac agtgattggc atgattgcta
    50881 gtttttactt tttgtcaaag gcaatcaaaa gtttgccgat gagtctctct tatcctatct
    50941 ggacagggat tggcgcagtt ggttcggtca taattggtgt ggtcttgttt catgataagc
    51001 tgaatctttc cacttggttc tttgtgggga tgctactcat cagtatcatt ggcatcaaga
    51061 ttactagcgg ccactagaca cttggaataa gtactgatca gaaaaaataa gcctttttat
    51121 ttaatattca atttttttat gaaaaaataa gaaataagtc caatgaccta gcgacttctc
    51181 cactcatcag gtgtatgctt gtactatcta tagaaaagga gtgaatattt ttgaaaagtc
    51241 tttcggtcca agaattatcc aagaactttg gtcacaaaca agccctcagc gatgtcagtt
    51301 ttagcttatc ccctggcaaa atcgttggat tagtaggtcc taatggcgct ggaaaaacaa
    51361 cgatcatgaa agctatttta ggactgatcc attactcaga tggaacaatc tgtattggtg
    51421 aaaatgagat ttctccctct agtcataaag ggttggaaca agtaggagca ttgatcgaat
    51481 acccgggtat ctatccattc ttaacaggct atgatcatct gaagttattc tctgaaacca
    51541 acgatgtatc tgctatcgac actatcgtga atcagctcaa gatggaaaag tacattcaca
    51601 aaaaagcgaa atcctactcg ttgggaatga aacaaaaatt gggaattgct cttgccttac
    51661 tgaaccatcc gtctatcgtt attttagatg agccgatgaa cggtttggat ccacaagcaa
    51721 caagagatgt gcgaaaaatc attaccgact tggctactca gggcacctct ttcttgatct
    51781 ccagtcactt actcagcgag cttgaaaaaa tggtcgatga gctgatcctg atcaatcaag
    51841 gaaagatcat caaacaatgt acaatggctg aattgaacca aacagcgcaa gatttcatcg
    51901 ttatctctac aacaaatgat ctagctgctc gaacgatctt gacagaagct ggatatactt
    51961 tggaagatcc tgataaaatc aagcttttaa aagaaaacga agaattatta ggaaccgtga
    52021 tccagctatt gtctgcacaa aatatccatg tgacagacgt ccagcatctg acgaatgatt
    52081 tggaatcttc tctattggaa attctggatt cagtaaaggg ggaagaagaa aaatgacatt
    52141 gcttgtaaaa caagaattat ttaaattgat caagaaaaag tcaactgctg ttttatctgt
    52201 tctattagtt gttttgctca taggaacagc acttctagcc aaaaaataca cgacgatcat
    52261 tgatccagtt gaaatgactg ctcagctttt tagtgccaca tcttggattg tctttatcat
    52321 gattgcagct gcaagcacga tcatttcgat ggaagcacaa tatggtacat tgaaaaatct
    52381 cctttatcga aagtattctc gaggagaaat attagtaagc aaatggatca cattagttat
    52441 ttattctgtt tatttatacc ttttagctat tattgtcact gtgttgatga aactgattct
    52501 tttcccttcg atttccttca cggagaaggt ttctactggt caaacactga tccaaagctt
    52561 gttcacttat actttgggta gttatatcgg tttatggctg attttaagtt tagtgttgat
    52621 gattgcttgc tttatcaata gttccggcgc atccatctca gcaggaatcg tcttttattt
    52681 tgcttcgtca atcatatctg gtatccttat tgcactcatc caaaaatggg agtggatcaa
    52741 atggaatccg atcagtatgt tgaatctgca aaaccaaatt ggaaacgaag agatcatgaa
    52801 gcaattgact catctttcta ctaatcaaat gctattcggt aatttagctt acatcgtttt
    52861 gttcttagca ttaggatatc tcgtatttaa gcgaaaaaat gtatgatgac tcatcaaaaa
    52921 agaggctgtg aaagagacgt tcagccttat tgccgattgg ctgtctctta aacaccgttt
    52981 attctgaaaa aggagctgtg acaacatttg tgtcacagct cctttcctag tatgtttttg
    53041 aataaacgac aggctcacaa cctctcttta tacttctttt atacttctat acgtgaagca
    53101 tcccagtggc aaataaaacc attgcaatca ctccgaccat cgtcacacct ataagtaaaa
    53161 tcttatctaa gtggatatgt tgtacaggct cgttttgctc cttacgtgct ttgaggtaaa
    53221 aataaatgcc tggcaagaaa agaacaagaa caagcattac ttcattaaag gctacgacta
    53281 gcatagccca tacttgaaag gcacttgcta aaagccctac taaaaattgg acccattctt
    53341 tccgctgata agaaagtttc atttggtaca atcctaagaa aagccacgtg aagaaaatag
    53401 ctgctgtacc aagtgtgtaa gcaaagttat aagcactgtc agtgaataat aatgaaagta
    53461 aaaagacatt cgtacataac gtcgtcaata cgatagcata agttggtgcc ccatttttgt
    53521 tgagttttcc ccatgcttta ggcaaaagtc cgtcttcagc catgtttccc attgtttcaa
    53581 tcggtaagat cgtccatgag agccaaacac caactaaaga aatgatcaaa ccgccgttga
    53641 tcacgactgc tccccattta ccaaccatca tttcaaacac atagcccata gcaggctgcg
    53701 ccttgatcgt ttgcagctga ttcatcgtca ttgtcccata tggaagcatg gaaaccaaga
    53761 cgtaaataat aatcaagccg aatatcccta gaatcgttgc tttcccaaca tctgattgtt
    53821 tttttgctcg tgaacctacg acagaggctc cttcaatacc aacaaatacc caaagcatca
    53881 ccatgatgca actcaatact tgactggaaa acgggatcgg ctgtccattc gcattcaata
    53941 taatgtcgct agaaaagtta tcaatgaatt gttggatcga aaacgtgctg atagctacta
    54001 caataaataa aaataatggc actaatttac agaatgtgac gatagcattg acgaaactag
    54061 cattttcgac tccccgattc actagccatg catatcccca taaaaagact gtactcatca
    54121 cgatcgatgc cagattttgc ccaccttcaa atactgggaa gaaatatcct aatgcgctca
    54181 tcaacaatgt tgcaaaagct acgttgccta accagcatgt gagccagtaa ccccaaccgc
    54241 tgataaaacc agcgaaacgg ccaaaacctt cttctgcgta actaaagatc ccgcctttta
    54301 aatctggacg ttttttattg atattattga tcgacaagga gagcatcaac attgctgttc
    54361 ctacgagtac ccaactgaaa atagctgggc cgccagccga tccggagatg tccgtcataa
    54421 taccaaatac gccacctcca atcgctgaac caattactaa ggcagcaagt ggaattaaac
    54481 caattccttt tttctgttgc tctgtatcca tgtaagcatc ctccaaaata aaaccgcttt
    54541 tatcaaaaag tataacaaac cttttttatt tttacttaat taataaaaac tttaatagaa
    54601 atataattct accggctttt ttctcattat acaagcaaag cgcctagtcc ctttccaaat
    54661 tacaccacca aaaaacttaa aaaagatgct atactaaaag gaaagaattg gaggatgatc
    54721 catgaacatt ttatatatat cgatttcagg aaatacgcgt gcctttgcca aacatctagc
    54781 agaatatgct gaaaaaatgc atgcagaaga tcctgtcaat cctgaagtta ctttaaaaga
    54841 aatccatgaa aatagtgatt ttacgaaaga aacagaacca ttttttactt tcgttcctac
    54901 ttatttagac ggcggaaatg gtttagataa cggtgataca gaaatcttga ccgaaacgat
    54961 gcgtgaatat ctggaacatg aagacaatca taaactttgt cttggtgtag tcggtagcgg
    55021 aaataaaaac ttcaataatc agtattgtct aacagccaaa caatatgcac aaacatttgg
    55081 ctttcctttc ttggcagatt atgaattacg gggaacacca tctgatgtga cacgaattta
    55141 tcagatttta gcagaaaaca ataaataata aaaaagaaga agctgaaaaa tagatttcag
    55201 tttcttcttt cgtttctatt gtttctatca tattaagctt tgacatccat cacaggtgct
    55261 tttgcttttg ctttacctgt tttttccaag acaaagtttt ttacacgatc catattggtt
    55321 accaagacga tcatggatgt ttctttgcct gctgctttga tcgcttctaa gtcagcttct
    55381 gctacaagcg tatcaggtgt cacttgctgc ccttctgtta ctttcacatc aaatggcgta
    55441 ccgcctaagt caactgtgtt gattcccata tgaagcaaga tttctaaacc atttggcgtt
    55501 gtgatcccaa tagcgtgttt cgttgggaag acagagctga ctaccccagt aactggagaa
    55561 tagatatgtc cgttttctgg ttctacaaag tagccttcac ccatcatctt ttgtgcaaaa
    55621 actggatcac ttgctttatc taaagaaaca actgttccgt cagcaactga tgagaggata
    55681 gtcacacttt cagctgaagt ttgaggtact actttttcag gcacttcatt tactgtggct
    55741 gtttcagtcg ctaattcttc tttttctaaa cggtctttag gaataccgaa gaagaatgta
    55801 gcaacgaagc caccagcata agctgccaat aatccaagga tgtagcctaa ccatttaccg
    55861 tcagagatca atgggatcaa agcggctcct gaaggtccaa tagcgatagc cccgacattt
    55921 ccgatcattc cgatcactgc cccgccgata cctccaccga tacaagcagt aataaatggg
    55981 cgtcctagag gtaatgttac tccgtaaatc aatggttcgc cgattcctag gatcccgact
    56041 ggtaaagctc ctttgatcat ttctgttaat tctttatctt ttttgcagcg aatccataat
    56101 gccaatgctg cacccacttg tcctgcacca gccatcgcaa gaattggaag caatagcgta
    56161 cgaccagttt catcaatcat ttgcacatgg attggagtca aaatttgatg cagtccgaac
    56221 ataaccatcg gcaagaataa tgtgcctaga acgaagccgg caaatgctcc accaacgcca
    56281 agtacccagt tgatcccacc gaccatgcca tcagaaatga aacctgctaa aggcatgatg
    56341 aagaaaattt caatcagacc gatggcaatc aatgaaagag ttggtgtaac gatgatatct
    56401 accgcatcag gtactacttt atgcagtttt ttctcaacga ttgacaatag ccaaacagcg
    56461 aagatgacac ctaggatccc accttgtcca gctgcaagag cttcaccagt aaataagttt
    56521 ttcagtggtg cttccggatt catccctgtc agcatcacaa cggccccgat aactccacca
    56581 agagttggtg ttgcaccaaa aacttgtgca gcattgatac ctgtatagat aactaggtag
    56641 ctaaacatcc cgtttttcaa gatgttcatc acatcgatat attgttgcca tgttgctgca
    56701 tcaagtgttc cggctgtgac taagtttgct aaaactgcgg caatccctgc cacgatccca
    56761 gtaccgacaa atgcaggaat cattggcaca aagatattag aaatgtcttt taataccgat
    56821 ttgattttag atggtttttg tttcgatttt tgtgccgctt tcatttcttg tgcccgttcg
    56881 tttacacggt cttttccaga tgctgatgaa tttgctgtag ctgtacttac agaagaaagt
    56941 ttttctccta atttcacgcc tgccaaatca accatttgct gtgctacttt gttcactttc
    57001 ccaggtccga tgatgacttg aagctgttca tcttctacta caccaagcac tcctggaatc
    57061 gcttttaatt cagagacatt tacaagatca tcattgatga tcttcatacg cacacgagtc
    57121 atgcagtgga caatgctttc tacattgccc atgccgcctg cccctttata aatttgtttt
    57181 gccagttctt ccaaagtcag ttcgtttttt gccatggtct tttccccctc ataaggtccc
    57241 ttttacaaat ccgtttgctt cagctaattt tgcttgagct tcctctttat cactatttgt
    57301 caaaatcatg acgatagcca gcttaacatt ttgatctgct tcttcaaagt acttttcagc
    57361 tgtttcgtaa tcagcttctg tcgcttgcat gatgattcgt tttgagcgtt cgattagttt
    57421 ttcatttgtt ggtttgacat cgaccatcaa gttattgaat actttaccta taccaatcat
    57481 actgatagta gaaatcatat tcaagataag tttttgtgct gttcctgact tcaatcgagt
    57541 agagcctgtc aaaaattctg gacctgcatc gacttcgatt gggaaatccg cgtgtttact
    57601 gatttcggct tgtttattgc aagaaatcgt tcctgtgaca gcaccgatcg attttgcgta
    57661 atctaatcca ccgatcacat atggtgtacg accgctagct gcgataccaa taactatatc
    57721 attttttgtc aaattcagct tgattagatc ttcttgccct agctcataag aatcttcggc
    57781 cccttcgaca gctacagtca ttgcttcttc tccaccagca attagaccta tgaccatttc
    57841 cggttctaca ccgaatgtcg gtacgcattc agcagcatct aacacaccta gtcttccact
    57901 tgttccggct cccatataaa tcagacgtcc acctttttta aaggactcaa tcgtgttagc
    57961 aatcactggt tcgatacttg tcagttcttt ttcaaccgca gcggccactt tctgatcttc
    58021 tttgttcatt agttgaacgg cttgagaaat actcatctca tctaatccaa aagtttcttg
    58081 gtttctgcgt tccgttgtta ggttctctaa attgattttg ctcattttta tcttccttct
    58141 tttcttataa attgatagtt cataccagct ttgatttggt tgatcaatgg tagctcttct
    58201 ttgataactt tagctactac attcactttt tcatccgctg gcaaatcata tttggttaat
    58261 tggatctcac ccatatagcg caaatacttt tcgttatcta aagtaataga ccctttcata
    58321 cgcgtagctg tatttcttgc tgggataggc ggaatctctc gaaaacgtgc atctacgctt
    58381 cgtatgacat ctcttgcatc gtcttgccga ttcgtatgct tccctaatat atattcgtaa
    58441 aattcttcat ctacaggttc cgtatggagc aagactgtct gttctttttg gaaagaagca
    58501 aattgctcct gaacgttttc tgacagacct gcatctccga tgtaaacaat atccgtgtta
    58561 cttgctaata gatctagagc gcctgcaaga ggatggatcc ctcgatgttc ttccaaagtt
    58621 ggcaatcctt gatatagtgg gcctctcaac tctgtatctc ctggtacaaa accttggaca
    58681 aggagacctt gaattttgag ccattgattt tttttctgat accattcttt gtccagccct
    58741 gtttcgggac gtggataata attatgccaa gcttcaatct gtgagaaatc agcttctgct
    58801 tctttcaatt catcgatatc tttaggcgtg atcgtgctag cattcaaact gattttcaat
    58861 tgatgtgacc agtcggcaat ctgctgattg gaaatatgat aatccattct taaacctgtt
    58921 acaccaattg ctttcaaagg cgttagatct tctaatgaaa aacccgctcg ttccaatgcc
    58981 tctcctgaaa tatcgaccat caattctagt ttattctttt tcgcttcgct tcctagttcg
    59041 atcaatcgct tttgatatgc cgaaacatca tcttctggta tatgcatgga agtgaaaata
    59101 ccgacaaagc cattggatgc catttggcgg atatactgtt tcttctcttc tgacaaagaa
    59161 tcgttcataa atattgaaaa gccaaacata ttatccctct ttttctgtat ccgtcttcat
    59221 ttctatgact agagtatata tctaaagaaa caaaatttca atacttttat taaaataatt
    59281 aaatttcatt tctaaaggtt gagtttatat tatcaaaaga attgtcataa cctaaaaaag
    59341 gcactgctaa gcaaaagcag tgccttgaaa tattttattt aaataacaca aaaacataat
    59401 tactcataag aaaccaattc tgtattatct accatgaatg taaagaaccc ctcattaggc
    59461 atcggatgaa aataagcatt gatcactctt atcaaggcat tatggcaaac taacagatag
    59521 acattttctt cgtcttcgat gcattctttc aataaaggaa cgatccgtgc atacacatct
    59581 aagactgact ccccatttgg gaatcgtaca gcgaacgcta aacgtgcttt ttgaaagttc
    59641 tcatctttgc ttggcagtcc atcataatcc ccaaaatcca tttctaccaa tcgttcatca
    59701 acggtcagcg gcaagctgag acgatcagca actgcttgtg ctgtatctct tgctcgttga
    59761 agaggggaat ggatgatttt cgtgattgga acaggcaaat ctactacctt ttctgctagt
    59821 ttctgtgcct gcatctgtcc tttttcagtc agcggcacat ctgcccgtcc gcaaactaat
    59881 ccttgtgcgt tccaagttgt ttctccgtgt ctcgttacat aaagcatgtg attctctcct
    59941 tcgttggcaa agttcccctt aaccatacct tgttgttctt gagattacaa gggttagatt
    60001 ctctcaagag aaataatctc agtagatact ttatccagga aggtttgatc atgttccact
    60061 agtaacatgg caggtttcac gttcaagatc agttgttcta attgttcttg attgaagaca
    60121 tccaaataat tcaatggttc atcccatgta tatagttcag ctggctgtga taaagattta
    60181 gccaattcca cttttttccg ttggcccata ctcatctgct cgatcttgtt atgaaaaaca
    60241 tctctttcca tcccaagctt tcggaggttg ttcaaaaatg cttggtagtc tacttggttt
    60301 ttctctgcaa attccgccaa cgttcctcga ttgtcttcat aattttggct tgcataacta
    60361 atgctcagat gttttggctg gtatttttct cctataacct tgccgttgaa tgcccccaga
    60421 agataatgga taatggatga ctttcctgca ccgttaggac ctgaaatcgc cacccgctga
    60481 tgaggctcga ttgtaaaatg aattggctcg aataacagat tttcataccc taattgaaga
    60541 tcttctacgc ttaaaagtcg cttatggtga gacgcttggc tattcatcgt caaagaatcg
    60601 atatattcga tatcttttaa tagtttttct ttctcgctga tctgggtctc catccgattc
    60661 acgattgctt tggatcgttt catcgtccgt gcagcatcag cacccactgc tcctttattc
    60721 actcgtctag attcagtatc gatgaatccg acttgtttct ttgttttatc tccttctcgg
    60781 gaacgagacc attcggcttt ttcagctgct gttttcttta gcctactgac ttcttttttc
    60841 aatttttcat tttgagccat ttcaaattca tcacgaagtt ttttctgttc ttcatagata
    60901 gagaaattcc cttgataaag ttccagttga cttttttcga ttgctaaaac atggtccact
    60961 acttcatcga taaatccccg gtcatggctg accacgatga agccttgttt tttctttttc
    61021 aaataagccg ctacttgttt tctaccagag atatccaaat gattcgttgg ttcatcgatt
    61081 aacgggaaat gagtgtcatc cacaaataaa agtgccagta ggacttttgt cttctctccc
    61141 cccgatagtg tgctgaattc tctccataag atttcaggat ctgtttgcat caattggagc
    61201 tctctttcga tttcccatat ctcaaaatcc gtaatatcat ttaacacgta ataggttaaa
    61261 cgttctttat cttttgtctg ttgcgggaaa taggcaaatt cttgctgatg gattacttgc
    61321 ccttgataag gtagtttgtt ttgtagaata ttcagtaaag ttgtctttcc tcgaccgttt
    61381 cgtccgataa gtcctagttt ccattgtgtg tcaaaattta gatttgcttg ttcaaataat
    61441 aatgtgcctt ggctgtcgta gccgaatgtc agatttttta tttcgatttt agacattttc
    61501 catcacctcg taaaaaaaga ccagtcctat tttcagaact ggctaaagag gatgcaaggg
    61561 ttggatacac acaaaaagac ccatacatcg aaaaaaccaa gccaatcccg aaaatctgca
    61621 cactgcttcc ttgtctccac aaggtacagt ctgaatgcag ctttcatcaa tcgacttaat
    61681 ttttcaatgc acggatccct cactttgtcc tatatttgaa attacgatag cataaagaaa
    61741 atgattttgt caactacttc gggaggatgg tgaaaaatct gctaagtgtc tgtcctaaga
    61801 tttttgtttt ttctaatgtt tgcctgtgtt ccattgcttc ttctaaacct attggaccca
    61861 aatgaatact taagacaatt gggagaaaat cagaaagtcc ttctagctct tggtctctac
    61921 ttccgcaaat agcgattatt ggttttcgat atttttttgc aagcactgct acaccgtatg
    61981 gaacttttcc gtggatcgtt tgctgatcga tccgcccttc tcctgtaatg atgacatctg
    62041 cttgttcgat ctttttttcc aatccagtga tttggctgac taaatcgaaa ccagaaacca
    62101 tcttcgctcc caacaaaaaa agggctccac caataccgcc tgctgctcca cttcctggaa
    62161 tatcatctaa atccacctcc cagaaatctt ttatttgctg agcaatcttt cctgcttgtt
    62221 tatctaagaa agctagcgtc gtatctattc ctcctttttg tcttccaaat tgccaagcag
    62281 ctccttgttg acctgtatat ggattggtaa catctgcagc gataatcaat tttactttag
    62341 aaacaagaac gttttttcga tcaaaatgaa gttttttagt agataataaa ggatttcctt
    62401 ctgtgtaccc ttctaaagaa gcacccaagg attggagtaa tcctagacca ccatcgctac
    62461 acccgcttcc tcctaaagtg acaataattt gttctacttc ttgaagcagt gcatctttaa
    62521 taagttctcc taaggcataa ctggaagcaa gtcggatagt ttgatccgtt ggtttatcaa
    62581 gtagatctaa ccccaatgct tgtgcagatt cgatgactgc ggtgtttttt ccttgccaag
    62641 ttgtcaataa atagctgatt tttcgctgac ggaataagag atccatccca tcaatggtac
    62701 gccatgttcc cctgagcgcg taatagattg cttctgcagt attttctccg ccatctgcaa
    62761 tcggcacatt gatttttctt cctttacgat cttcccagcc atttagaact gcctgattta
    62821 gttcaaatga agaagcactt cctttaaatg aatcaattgc cgccaaaata tccatctgta
    62881 tccctcactt tcttcttaag taagacactt tttttcattt ttttcaaaca aaacaatgct
    62941 attttcttcc atttttctat atactaaaag ggaactcatt gaataaagga gagaaaaaac
    63001 atgaaaatcg caatagctgg ggccggagca atgggcagcc gaatcggatt gatgctacat
    63061 caaagcggaa atgaggtcct cttgatcgat cgttggcctg ctcacattga agccattcgt
    63121 acaaatgggt tgattgctga ctttaacggt aaagaagtgg tcgcaaaact gccaatctat
    63181 tcaccagaag aaatcatcga aagcaacgaa cacgtggact tgatcgtagc attaacaaaa
    63241 gccaatcaat tagatgatat gttttgttcg atccaatcca ttattactga caatacttat
    63301 gttttgtgtt tactaaatgg attaggtcat gaagatgtac tggaaaaata tgtgccaaag
    63361 aaaaatatct tatttgggat cacgatgtgg acagctggtc tagctggtcc cggaaaagtc
    63421 acattgttag gtgatggaga aatcgaacta gagaacttgg aaccagaagg cgaagcattt
    63481 acaaaaaaag ttgtagaagt ctttcaagaa gcaaatttaa atccgatcta tagccataac
    63541 gttcgttatt ccatttggcg aaaagcttgt gtaaatggaa cattgaatgg tttatgcacg
    63601 attttggatt gtaatattgc tgaactaggc gcgcaaaaag cggcagagag catggttcga
    63661 acaatcgtat ctgaatttgc ttctatcgca gcaaaagaag gaatcatact agatcaagaa
    63721 gaagtgtacc aacatattgc ttctacttat gatcctgaca acatcgggtt gcattaccca
    63781 tctatgtacc aagacctgat caaaaaccat cgtttgactg aaatcgacta catcaatggt
    63841 gccatttggc gaaaaggaca aaaatatgat attgctacac catattgtgc ctttttgacc
    63901 caattagtcc atgcaaaaga aggaatcctt ggcgcagaat aaaaggctct ctgatgagaa
    63961 tcagctgaat cagcagctgg ttctcttttt ttaagcccaa taaaagatca aaaagaaaac
    64021 acaactctca tgatgccata agaattgtgc ttgtttgttt cagtacataa tctcttcaaa
    64081 atgtaggttt tttatttgtt ttcaacagag gagaggtcat attcgtattg ctcataaata
    64141 gtggaatcgt tagtgataaa tcgttttgcc caattttctg catcataaaa ttccaatagc
    64201 tctgatagga ttggcttaaa tggtgtatgc agaaattccg gtaattcatc taaatcacgt
    64261 tctcgaagca aaaaaatatt ttcaggacga taaaaatgat aatgagcaat cgcttgatta
    64321 tccgtcacta gtttcttttg aaaatacatg gattcaaatg tccgcaaagt cactccttgc
    64381 tgaccctccc gcaacagttc cagaatcccg cgcgattccc ctgttttttt caggtactct
    64441 tcataagtaa gatatccagg ttgttcattc cctcgatgtg gaagaatgaa atagttggtc
    64501 gtaacatctc tttttataaa ttcctgctta tagctttctg ctcgttcttt tctcccatta
    64561 ttcgttgcac caaaaaatag atccgtcttt ggttcgctag aagaaaaatt catatgtttc
    64621 gaataaaatg aactattatg tttcagccca agttcttttg cttccaaaaa atcaaaatga
    64681 taaatctcat cgattgccgg ttcatcttgt gcctgcttca gcatcgcctc gtgttctgct
    64741 ttgaagtgat tccaaaaaaa taacacaatt tttgcattgg taaatgggcg cacgcgtcga
    64801 gccatctcca tatcaaatcc ataatctaaa aaaacgatca gctgataatt tgccaactgt
    64861 tgtctagctg atttaatgaa ttccgactgt agtaaatctt ttccggacca aacatctaaa
    64921 gagcggatgg aaggcaaaaa ataaagtgat ttattttttg caactagcag tacgtcattt
    64981 ttgttcatct tttccgcctc actttctctg tcactttttc ctatgatagc ctgtttaaat
    65041 agtaaaaagc cagtaaaacg tttcctatat ttgtatgaaa atttaacatt tgtatgaaaa
    65101 tatgatgaaa taaatcattc ttctattaaa attcgcctaa aagttttctg aatagttgta
    65161 attgtctgaa aatttgttat cattagtcat tgttaaatat tgctatttat tcttaatata
    65221 aattttgatt gtgaatttaa aagaaagagg tcatttatgg aagataaaaa attgaaaaaa
    65281 caacttagct ccagacatat caccatgtta gctttaggtg gagcaatcgg tgcaggtcta
    65341 tttaaaggaa gtggcgaagc gattgggatc gccggccctt ctgtattgat tgcttttttg
    65401 attggtgggc tgattttata tatcgtgatg aaaggtctgg gaaaaatcgt tttaagcggt
    65461 ggcgacacgc atcacggatt atctggtttg atacgccctt atctaggtgc tcattctgct
    65521 gattttactg attgggtcta ctggtctatg tggattatca atatcatcgc tgaagctgtt
    65581 gcagctgctt cctttttgca gttgtggttc ccgcacattc cagcctgggt atttgttttt
    65641 atgttggctg ttttaactac gatcatcaat ctttattcgg tacggttatt cgcagaaaca
    65701 gaatattggc tggcttttgc taaaatcagt gtcattatct tactgatcat ttttgctact
    65761 tatcttgtcg gtcagcaaat gttgggcaca ggcgttttcc ctacccttca gcaattgact
    65821 gaccatggtg ggtttactcc tcatggtatg aagggaatca tcagttcttt gcttgtagtt
    65881 atctattctt acggcggttc tgaactgatt gctatcacgg taagcgaagc agatgatcct
    65941 aaacgtgcga ttcctaaagc aatcaaaggt gtcatgggaa gaatcatttc cttttacatc
    66001 attcctttgt ttttgctttt gattcttttt ccttggaata ctttagcagg cacgaatgtc
    66061 agtccttttg tgatggtatt tgaaaaaatg aatatcccat ttgcagcgga tatcgtcaat
    66121 tttgtgattg tccttgcttt gttctcatct atcaattcgg gtgtgtatgc atcttcaaga
    66181 attttgttct tccgtttgaa ggatcgaaaa ggttcctcta gaaaactagc tgttttaaac
    66241 aagcatcaag taccgcaacg tgctgtttta ttttgtacca gtgttcttta tcttggtgta
    66301 gctctttctt attttgtcgg tgacaaatta tttggctatc tggcaggttc cctttcgtat
    66361 acagtattat tgatctggat cattatcagt gcggcatcct ttgtactggc actaagaaaa
    66421 ggaacattat gggataaagg aatcagcttt tttgcattag ctgtcttagg attgattttc
    66481 cttggtatcc tgttcacgaa ctccattggg gtaactgtat tgactggcct gctttacctg
    66541 ttcatttatt tcagttatca aaaaaagaat gatgcgttcg tattgacgaa tgaatgaaat
    66601 cgatagaaaa ttttatttta tttatgtgga atagatggag ctattgaaga aaggagttgt
    66661 gacaaaagtc ttgttgcaac tcctttttcc gaataaacag tctcatgacc tcttggtaaa
    66721 cggtgttcaa aaaccctctg tgcaaaataa ttgattgttc tccatgctca tgacatgata
    66781 gaagaaagga atgaaaaaaa ggagtttgtt ttaaatgaaa aagtattgga agtttttgtt
    66841 gatgatcggt gtttcaacgg tagttatgtt tggtatgatg tacttgaatg tctatgcgct
    66901 cgatcatatc ttcttcagtc gtacacgagt attcatggcg ttgatgatgg gtgcagtcat
    66961 ggcagtcatc atgctgttgt tcatgtggaa aatgtatgaa aacaaaaaga taaatgttat
    67021 gatcttagcc gctagtcttc ttgtttttgc cggttccttg tttatggtac gaagccaaac
    67081 tactgtcaac gacacagctt ggatgaaggc catgattcct catcattcta tcgctatcct
    67141 ggcaagtaaa cgagctgatt taaaagatcc tgaagtaaaa aaattagcgg aagaaatcat
    67201 tcaggcacaa gaagaagaga tcgacaaaat gaaaaaaatg atcaaagaaa tggaatgacc
    67261 attatccgtc caaataaaaa aagaggatgt tccagaagtt atcagcttca agaaataaga
    67321 agaagctact tctggaacgc cgtgaataag gggttgtgaa aaagctgccc tgcatcaagt
    67381 aataagaaca gccaaacaaa aatagcttct cctatttttc gtttggttag gcttattacc
    67441 gcagatgcaa gcttttgaac accgtttatt aagaggttgt aaaatcagcg ttctgacctg
    67501 agtattagag agacaaatgg aaaaatagtt cctcatattt tgccattttt tgagctaata
    67561 ccgagggtca gctgattgaa caccgtttat cgagaggttg tgaaaaagtc gcttagtttc
    67621 gcgtattaag aaaaattttg tgaaatggct ttccacattt ttgtataaat ttggcttaat
    67681 accgagaaac tggcttttga ataccgttta ttcgtaaaag ggattgtgac aaaagtcttg
    67741 tcacaatccc tttttatgtt tttattgttc taattgcgct gtcttgctta ctgcttcttg
    67801 cggtttcaca aatttttttg agattcccca acctgtgaag gcactgacca tcgaaaacac
    67861 aggagaaagc aaactgaaaa atacgaatgg caagtaagtg agtgtaggaa taccaaatgt
    67921 acttgctacg aaacttccgg caattcccca aggcactaag tagttgatta ctgttccacc
    67981 atcttctagc gttctaccta atacagttgg atcaaatcct gctttttcat aagtttcttt
    68041 aaatgcatta ccaggcagaa taacagatag gaattgttca ccaataaaaa tatttactcc
    68101 gatacttgtc atcaatgttg taaaaatcaa atttggtgtg ttcgtcatct ttcttgatac
    68161 tcgttccatc acggttgaga tcaagccaaa ttcgatcaac aatccaccta atgacaaagt
    68221 tagaataatc aatgacacag taggcatcat actttcgatt cctccgcggc taagcagtga
    68281 atcgatttgt tggtttccag tatttgaaac aaaccctgat tcgatgaccc cagccaactt
    68341 cgttaaggaa gtttgtgggt tttgaataaa aatcattcct actgctacag cgatattcaa
    68401 caaaatcgtt aaaattgccg gcaccttttt ccatgcacac agtaacatta aaaataaagg
    68461 aatcagtgac cagattgaaa tagtaaagtt tgcttctaga acatcagtca cttcacgaat
    68521 agatgaaaga cttgcggctt tacttgtgtg tcctaaaatc atgaataaaa agaaagaaac
    68581 aacaaatgcc gggacagttg accacatcat atgcttgatg tgtttgaaca aatcggcatc
    68641 aacaatagct gctgctagat ttgttgattc ggataatgga gacatcttat ctccaaaaac
    68701 ggcacctgaa acaatagctc caacaaccag cgctggattt aagcctagtg ttgtcccaat
    68761 accgaaaaat gcaatcccaa tcgttgacat aacagtaaag gcactcccta cagcgctacc
    68821 tacgattgca cacacaacaa atacagaagg gacaaaccac tggacactga tcaatttgaa
    68881 cccaatgacc attagagttg gaatgatccc tgcttgtatc catacagcaa tcaatgaacc
    68941 gactaaaata aaaataaaga ttgggatgat tcctggtttg atgccattga tgattccttc
    69001 gtgcagccaa tctgtcgggt atctttttat cactgcatac aacatgacca ttccgatcgc
    69061 tgctaagatc gtgatattag gtgagacctt taatccgaca acgccaatcg caatgcctaa
    69121 aacaataacc attaatacaa gtgttgcttc taaaaaactt ggtgttcctt tttttcccat
    69181 gataatctct ccttttttag ttgttcttat gttctaaaaa agttaagata tcggattact
    69241 ccgccgcaat tcatccgcga ttcctccttt ataaaaataa aaagacctgt tttaaactaa
    69301 ggacgaatga ctccgcggta ccaccttaat taaaaacaga tctgtttttc actttcaaac
    69361 ttttattcaa atgtatggac tttccatgta attcgtcaat taggcttgat cggttcacag
    69421 cgaccaccga ctttctgaac aaaacaccta accgctactg cgtgaaaagt tgacttgtac
    69481 gaagtttacc ttccataaga tcatttgtca atgaaaaaga aaatattttt tcatttttct
    69541 gaaaattttt tttataactt tcacacacaa gtttattttt tactagaaag tagcgccctc
    69601 ttgttttttt cttgctacta tttcttgatg ttcttgcttg attctttcta atagctttgg
    69661 ctctttcaac agatcaagag ctgtattggc gagtagttct gctgcaatcg caatagaagc
    69721 aaggcctttg tcacttttag cagcagcttt gaattcttct gtgtgtccgg caatatatgt
    69781 ctcagagatt gctaccgtag gctggattgt tggtatgact tggctgacat ttcctacatc
    69841 agaagaaccg agacttgttc tttgttccgt ttctatttct tcatctggaa cacctgcttc
    69901 ttttgcatgc tggaagaata agtcatcaaa agaaggagta acgatgacat catcaactgc
    69961 attttggaac aatccaaact caaaagtcgt gcccgtactt atcgcagcac ctcgtacgat
    70021 attttccact ttttcatata ctttatctaa tgtttgccga tttcctgcac gtaaatagaa
    70081 acgtccagca gcgtaatctg gaatgacgtt ggcagctact cctccgtctg taatgatccc
    70141 atggatattg acatcttttg gcaattgcaa gcgtagagcg ttgattccat taaacacttg
    70201 gatcaatgct tccaaagcat ttactccttt ttcaggtgct gctgctgcat gggaagcgac
    70261 accgtaaaat ttgatgtcga ctggatcatt cgctaaagat tctgtcgttt tcccataacg
    70321 aaaagctgga tggacgcata acgctacatc gacatcctca aaaaaacctt ctcgaacaaa
    70381 gctgccttta gctgatccgt tctcgccccc ttcttcccct ggggtgccat atacacggat
    70441 ttctccacca gtttcatcaa tgaattgctt cactatgctg gcggctaata cggagtaagt
    70501 accaaacaaa ttatgcccgc atgcatgacc aatacctggt aaagcatcga attctgccaa
    70561 aaaggcaatt gtgggaccgg gtttattgga aatataacgt gcatcaaagg ctgttcggtg
    70621 tccagctact tctttttcta cccaaaacct ttctttttct aattgtttaa tcaacgctgc
    70681 tgaggaataa aactcatagt tacttacttc aggatgttcg tggatatcta gagcaagtgc
    70741 ttgatattct ggtaaacgat attggattag atccttcaat tggtttgttg agattgttgt
    70801 tgtcataaaa aattcctcct atgctagtca tttttcttat tttctcccga atgtttccca
    70861 tgcaggcacg cttgatcctt ttgatgtttt ctcgattact ttgactgtgt cttctgtttg
    70921 ataagcgtct actatttttt gatacgtttt attgttttca tcttcttttc ttgcaacgat
    70981 gatattcaca taaggacgtg catggtcatc cactggttct aagaagatgg catcagaagc
    71041 aggattaaat ccagcatcta cagctacacc gctattgatc actgaaatgt ctacatctga
    71101 caaagcacgt gctgtttggg aagcatctaa ttcagaaatg tctagatttt ttctattctc
    71161 ggtaatatca gaaacagtgg gcgcttgtcc ttttgcctta tccactttga tcaatccagc
    71221 agtttgcaat aacagaagag ctctgccgcc atttgtaaca tcattaggga ttgctacttt
    71281 tgctccgtct ttgatatcag ctacatcctt caaagtgtta gaataaattc ccagcggtgc
    71341 attgacagta tttccgatcg atactaaatc agttccgtgt tctttattgt aattgtctaa
    71401 aaagaattga tgttggaaag aattcaaatc aatcgatcca tctgctaaag ccacatttgg
    71461 ttgattgtaa tctgtaaatt tcacgtattc caaattaatg ttctcctttt tcaaacggtc
    71521 tttcacgttg tcccatacat ctgtatcttc tccaataatc cctagtttta cgatagtttc
    71581 ttttttgtca gttgtatctg cttttccttg gctacaccct gccaaccaaa taattgctac
    71641 aaatgtaatg actacaaaac gaataatttt tttcattttt tctcctccac tttcaatgtg
    71701 taattttttt aatcagttga ttactgataa attgacttaa aaaaactaag attagaatca
    71761 ataatgtagc cacaatcgtt acatctgttt ggaatcgatt ataccctctc gatatagcaa
    71821 gattccctaa tccgcctgca cctactgctc cagccatggt agtcagtccg atcacgttga
    71881 tgatagtcaa cgccgagacc cgaatgatac caggcaaacc ttctaacaaa tacacacgta
    71941 aaatgatacc gattggactg gttcccatcg cttctgccgc ttcgatcacc ccaggatcaa
    72001 cttctagcag tgcgttctcg atttgacgag caaaaaacgg aacaactcca aaaatcaatg
    72061 gcactaaagc tgctttcgca ccaatcgtcg ttccaactaa gaaacgagta gtcaatgcta
    72121 aaagagaaag caagataatg aaagggatgg aacgaatgat attgatcagc ttatcaagta
    72181 tttcaaatag cagacggtta gctagtaatc cattagggcg tgtcactact aacgtcacac
    72241 ctaagatgat tccaatcatc ccgccaatga atgctgtcca aaaagacata tatagtgttt
    72301 gcttcgtcgc ttcgacaaat tcatccggaa tcaaataaac attgggcaaa tatgttttaa
    72361 agaaatcagt cattttatct tcctccttcg atcactttga tacgactatc taaatcgttt
    72421 ttttgtaaaa tatttatttt tacaccagat ttgatcaaat aatccaatgc ttttttccgc
    72481 tgtttggttt ctccagctaa agtaacgatc aaactcccta acggcgtttc ttgtaataac
    72541 tctacattgc catataaaat attcgctgtt acttgatatt tgctgtacag atgagcgatt
    72601 agaggttcgt tcgtctgatt tccaatataa cttaattcca ccagccattt attggcgatc
    72661 tgatcggcaa aagcagaatg ggcaatgatc gtttctaatg cttggtcaag atgagtagct
    72721 gttcgaataa aatctttggt caattcttct tcaggattgc tgaagatctg gatgctgcta
    72781 cctttttcaa cgattcttcc atcttccata accgctacct tgttgcagat ttctttgaca
    72841 acttgcatct catgagtgat caggacgatt gttaaaccta actggcgatt caattttttt
    72901 aacaatgcaa ggatctgaag ggtcgttttc ggatcaagtg cactggttgc ttcatcacat
    72961 aataatacct taggttcatt cgccaaagct cgtgcgattg ctactcgttg tttttgtccc
    73021 cccgacagtt gtttaggata agcagatgct ttttcttcca aaccgacaag ttccagtagt
    73081 tcattcactt tttgacgacg ttcctgtttc gattttccag aatactttaa agaaaaatca
    73141 acgttgtcaa aaatagttcg gctttccatt agattgaaat gctgaaagat cataccgatg
    73201 gcctttcttt cttttcgcaa ttctttggga gagagctgac tcagattcgt ctgattgaca
    73261 atgacttctc ctgctgttgg tttctgcagc aaattcatca cacgaactaa cgtacttttc
    73321 cctgcacctg agtaaccgac gattccataa acatcgcctt tttcaatcgt taaatctaca
    73381 gaatctactg cctgtatctc tttttctttt tgtttgaaac atacagaaac atctcgcaat
    73441 tcaatcatta aaattcctcc ttatctaaaa aaagcgtcct caagaaaatg aaaatgtcat
    73501 tttcttgagg acgctttttt gcgtgttacc acctcatgtt caagctgtgt tcacacacaa
    73561 ctcctcttca agtacgatca tactctagca agatatcggt cgcagccgtc atcgccttac
    73621 atctatgctc gggatgaatt gctcgatggc cattttccaa tagtcgttcc ttgcttcttc
    73681 tcagctaccg aagctctctg tcaaaggttc ttctatcgta cttaccattt caatgcaaat
    73741 aatattcaaa aaaaaagtcc ttttactaac ataaactatg tcagtaaaag gacgacgtat
    73801 cgcggtacca ccttttttcg gaagtttcct tccctcaagc ccaacatctc aaaaagagag
    73861 attggcgttc gttttaacgg tcgaacagtt ccgaatttgc ctacttatcg acaaattatt
    73921 ctccaaggcc attttcagct tttcttactt gactcttctc accagccaga gcctctctgc
    73981 aaagtttcca aacttacttt ccttttcaac gaaatcgata ttttgttgtt gggattaatc
    74041 tacatgagga aaactgaaaa gtcaagtacg aaattcaaat tattttttct atatttattt
    74101 ttctgttttt ttatttaaca tttcaataac agttcgaaga tcttttcctt ccgcaagaag
    74161 tttcaatttt tccttttctt cgacggtata atttcggaca aaactggaat caggtatcaa
    74221 aacaacgcct aatcctgctc gtgaagcagc tgttgctcct actaaagaat cttccactgc
    74281 aaaggctgaa ctagcttcta atcctgttcg ttccaaagct gtcagataag gaatcggtga
    74341 aggcttgttc tccgacactt cgtctccgaa tgtaaacgtg tccaagtaat cagctaattt
    74401 aaagtgatcc aagatatctg ttgctctttt cgtaacagtg gtcgtagcaa tgcctagttt
    74461 catatgacgt tcttttaagt ctgccaatat ttcttttgca tatggtttta agtggatggc
    74521 tccgcttgtc agctgttgat aaaaatattc ttcacgcttc gctcggactt cctgtacttt
    74581 ttcagggcta cctgctgctt tcactaaaat atcaaaactt tgtttccagt tttttccgcc
    74641 ccaagcgtca acgatttctt ttgggatatg gatatcaaat tgctgaaatc cccaaagcca
    74701 gccatcacga tacgtttttt ctgtgtcaac taacaaacca tctaaatcaa atattgcacc
    74761 tttcatctat acattaatcc tttccaaaac aatacacata gtttagcacg actggtccaa
    74821 aaagaacaga agcaaagaac cagccttctc gtttataaga gttcttggaa tcgacggata
    74881 tagattttct tcacagctgt cacaagcgcc agataagcga tgactgtagc aaataaccat
    74941 aaccagtaat tgccaggaag tggcatcaag cctaattcag caccaaaact tgtaaaagga
    75001 aggacagaac cgacaataat tcctaatgta gtgataacaa acatggcaaa tgttgcgttg
    75061 ctttggataa atggtacttt cggtgtacgc agtgcatgga gcactaaggt ttgtgtccat
    75121 agtgattcga caaaccatcc tgcatggaat aatgcgatga aaatcactcg ctgtgaacta
    75181 tccaatgtat ggaaacttcc gcccactaca gcaggacaaa taacaaaata catcaacaag
    75241 taagtagtaa tatcaaatat cgaacttgtt ggaccaaagt aaaccatgaa ttccccaata
    75301 ctagatgcat tccatttttt tggctcgtcc aaatattctt tatccatccg atcccatggc
    75361 aaggaaatgc aggaaacatc atagatcaga ttcaaaaaca aaagttgtaa aggcagcatt
    75421 ggtaaaaatg gcaggaacgt acttgcaatc agcacggaaa acatattgcc aaaattagaa
    75481 cttgctgtag ccttgatata tttcatgata ttcccaaaag tttctcgtcc agaaagaagg
    75541 cctctttcca aaatcgtcaa atctttttct aaaaggatca cgtctgcaga ttctttcgca
    75601 atatccacag ctgtatctac agaaataccg atatcagcca ctttcattgc aggagcgtca
    75661 ttgataccat ctcctaaaaa tccgacagta tgaccatttt tacgtaaagc tgtcgtcaaa
    75721 cgtgcttttt gctgaggatt caattttacg aatacttcat ggtcttcagc tacttgtgct
    75781 aattcatgat cactcatttc cgagattttc tcaccggtaa ccaattcatt gatctctaga
    75841 cctacttgac ggcacactga acgtgtaact aattcgttgt ccccagtcag aacttttaca
    75901 gctacaccat gttctttcag agctttcaac gcttgtttcg ttgtttcttt tggtggatca
    75961 aggaaagcaa gataaccaat caaaaccatg tctgactcat ctttgactga aaattctcca
    76021 acgacgcttg gattggtctt ttgtgcgaca ccaatcactc gtaagccatc ttcattaagt
    76081 gactgtactt ttgcaagtac ggcttctttt cgttcttttg ttaatggtgt aacagtaccg
    76141 ccaagatcaa taaatttaga aatgttcagc atttcttcaa tcgcgccttt tgtgatcatt
    76201 tgtgttttac cagctggatc agcaaccaca acactcatac gacgacgttc aaaatcaaaa
    76261 ggaatttcat ccactttttg ataattgatc ttatctgtat ctaaagtttc tgttgcttca
    76321 tcaatgatag ccacatctaa caaatttttc agccctgttt gatagtagct atttagatac
    76381 gcatggcgca aaacacgatc atcttcattt ccttcacaat ccatatgata ttcaagaatg
    76441 attttatctt gtgtcaatgt ccccgtttta tccgtacata acacatcgat tgcaccaaag
    76501 ttctggatgg cattcaaatt tttgatcact gttccttttt tggccatcac acttgctcct
    76561 ttgaccaaat tcgtcgtaac gatcattgga agcatttcag gtgttagacc aactgctaca
    76621 gatagtccaa aaagaaaagc ttctagccaa tccccttttg taagcccatt gatgacgata
    76681 actgctggag ccatcaatga cataaatttg atcagtagca tggatgtttt atggatgcct
    76741 aattcaaagc ttgattgaat tggtttttca gatagcgttt ctgctacatg accaaatagt
    76801 gtttcatttc caacagaaac aatgattcct tccgcacttc cactgatgac attacttccc
    76861 ataaagacaa gattttcgta acttgtttcg ctttgctgat cgtcttcaca tactttcgca
    76921 tgcttttcta ctggatagct ttcccctgtc aatgctgctt gggaaatgaa taaatccttt
    76981 gcggacaata aacgcatatc cgctggtatc atatcccctg ctgaaagccg aacaagatct
    77041 ccacagacga tttcttccat aggaatctct gtgtactttc cattacgttt tacagctgcc
    77101 gttactttca caagtgattt cagtttttca gccgcttgat tggaacgtac ggactgaatc
    77161 aacgtcattg tcccgctgac gataaccata gtgaagataa tggctacacc gaaaagatct
    77221 ttatctccag gagctgctaa tacatattcc gtgatgaatg aaataattcc taatgcgatc
    77281 aacactaatg taaatggcgt gatgtatgct ttcaatattt ctacaatgaa tggtgttttt
    77341 ttgccatatg aaatcgcatt ttcaccgtaa ttttctcttg aacgttcgat ttgttcttct
    77401 gtcaatccat ttgctgatgc atggaatagt tcaaataatt cttgtttcgt ttttccagca
    77461 aaagctgcat agattttttg tgtatctttc tttttttcca tctcaatctc tcctttgtgt
    77521 ccaaaagaca gctgttctcg ctgcttttgg tctttagcta ttcatcggat cgatagagaa
    77581 aaaactgaaa ggggctgaga acagatagat aatagccctg gcctttcctc tagatccttg
    77641 tttttcgaat gactgtcatc catttgttct catctccttt actaagtaat tttaattcat
    77701 gacttccaat catttttcga atagttattt ttccaattag accacctcct ttttattttt
    77761 caccgatggg gatttgcggc aacaaaaaaa cctctcgcca aatactcagc gaaaggtttg
    77821 aaaaagcata ttaaaaaaag cttctcagat ctcatcgttg agttttgaca ctgtacaacg
    77881 taaaaagcag atacctgctt ttagctacct tatgaaaagc cttatctcga caattcctgt
    77941 tctacccatt ggcgtctctc gacgtttttg ggcagtagcc tatgtttgta taggagcctc
    78001 acctaacaaa tcttattcgg ttttccttcg ttaatataac aactaaaaaa gaggatgtca
    78061 aatcaatttt ccatgttttt catacttttt ttaaaataca cacgttatat tcctgtctca
    78121 cgcggtttta tttgtctcat tttccacctc ccgctataat agaaatatag taggaaacga
    78181 ggtgctgtta tggaaagaac acttgttatt atcaagccag acggcgtgag acgccattta
    78241 gtcggaagca tcatccaacg ttttgaagca aagggactgg ccattgctga aatgaagttt
    78301 gaaactatga ctcctgaatt agcaaaagaa cattatcagc acttgactga gcgcagtttt
    78361 tttgatgaac tgatcgatta catgacttcc ggtcctgtcg tttatcttgt gctggtcggc
    78421 gaggaagtga tcgatatcgt ccgtaagatg gttggagcaa caaaagctgc cgatgctgtt
    78481 ccaggtacga tccgaggaga ctacgcgctg ccggggactg aaaacatcat tcacgcttct
    78541 gattcccgtg atgctgcggt aaaagagatc gcccgctttt tcccgccatt cgatataaac
    78601 aaaccgtctg aaaaagcggc gaagtgatac aaattctaaa ctgctggtct agttgctggg
    78661 ttcagcaatg gccagcagtt ttttctctaa ggacctccgt tgattctttc ctaacatata
    78721 ttcgagcact gtgaagaatt cttggtagtc tttcgtcttt tcttccccta aagcagcaga
    78781 aatcaatctt tgataataat ctgccatcaa tgtttcatag atcactgtac ttctttcttt
    78841 atgtgctaag ctccgtttca tatcttcatt cacactacgt gcctttttca accgaccatt
    78901 ttctatataa aacgaagtca ggttcattaa aaaagtaaag agatactgct catgattgtt
    78961 caactgtgct aatcgtcgca ttcttttttt gattttcaaa taattgttct caatgaaccg
    79021 atcactgaat atccaaggaa aatcagctag aatagatagt tcaaattgac catagtctgg
    79081 tatctgcatc aagtattctt caatgattcg gcattcataa cgtaaaaatt caaattctgt
    79141 ctcagatact tctgtttttt cgaccatgtg aagattcaaa accaaataat acagatcttc
    79201 tgttttctta aaatgcctac ggttattgct gatctcttta taaggatcaa agctgtcttc
    79261 ttgtacccga tctagcattc gtcttttttt ctttaacttt attggttcat ccagctggtt
    79321 tcgcatccat tcatattctg caagtgtcat gttcattcgc tctaaaaatg atctcaatgt
    79381 tacaaatgac atttttttca ctgaatttgt ctcaatcccg actagagaag tcttaaaaca
    79441 gattccttcc gctaattggg actgtgtcaa cttcttgttg actcgaagtt tcttgatcaa
    79501 ttaaccatca tacataatag ttctcctttt taatttaaat ttttccacat tcaataaata
    79561 gatacgttaa aaaggaaaaa tacacgaaaa aaataaaaaa atgttttttt cataaaaaat
    79621 accaaccttc aagcattaga atgttgtcta actactgaag gttggtttga ttgtttattg
    79681 attatttttt tgctgttcaa cgtctgttct tgttactgga tataagttgg ttgttgtttt
    79741 attaccattt tttgaaaaga cacttgtcga atgttcgcct ttttgctttt cgattgcttc
    79801 attacgttcc gcttcatttt tatagtcata ttttttcgga tcaatcgtct caaatccgtt
    79861 tggcacataa aaccgcaaga gattttcctg attcagctta tctgaaagac ttaacgcagt
    79921 cttgactttt tcctgatctt tggagatttc ctcatcgatt cctgctgctt tcgtatcgat
    79981 gatttctcct gtctgattat tgtaagcttt tcctcctagg acggtgtatt tgggtgtcac
    80041 aaagttccca tttcgaaaag caactacttg atcatgctga gaagataata agtctgtacc
    80101 aaaatgaaga taatctttat catcgattcc caatagatgt agtaatgtcg gcaaaacatc
    80161 gatttctcca ccgtattcat gattgactgt ccctttagta taaccaggca tatggatcat
    80221 caatggcact cgttgcatct gtgcgttgtc aaattcatcc catgtatctg gatctttacc
    80281 aagagcgctt gctaaatctt tattatctgt attcgagatc ccaaaatgat ccccgtaaat
    80341 cacaaagatt gaattttggt aaataccaga ttttttcaaa tacgtaaaaa attgttccaa
    80401 tgattgatcc agataatgtg ccgttcgaac ataattatct actgtgctat tgccagtatt
    80461 caaagatgga aagtcgaaat tcttgtcatc cgtataataa ggcgtgtggt tggttacgga
    80521 aagaaatttc gtgtaaaacg gctgttgtag atgctcaaga tattgggcag attctctgaa
    80581 taaatcttta tctaatatcc cataacctaa cgtttcgtcg gattcatcaa aataagatcg
    80641 atcaaagaaa ttgtcataac ctaagttttt atatacatga tctctattcc aaaaactagc
    80701 tacattccca tggaaaacag cgcttgtata gccttgcttt tgtccaagga tcgctggtgc
    80761 ggcttgaaag acattgtctg acccaagttc tgtgaataat gagccttgag gtaaaccaaa
    80821 tgtgccagtt tccaacatat tttcagcatc acttgtcttt ccctgtccta cttcatgaaa
    80881 gaaattgtca aagctgatcg ttgcttgatt ctggaaaata ctatttaaaa acggtgtgac
    80941 ttcttgtccg tctaccttca tattaatcaa aaactgctgg aaactttcta aatggagaac
    81001 gatcacattt ttccctttag cgatcccaaa tgtttcggga tttggctctg cataatgttt
    81061 ctttgtataa tctaaaactt catcaatccc attactgctt gcatgcgcgc gaacactgct
    81121 agtcattcct gttttgattc cgtcataaat agtatacgca tcgattccta gatatttgac
    81181 aatatatgaa cggtcaaatg ttcgctgcag caattgtgga cggttggctt cgctcagcgc
    81241 tagatttcct gaaaaaataa gccctgaaag acaggtaaca gcgatagcca tcggtttgcc
    81301 gacagggttg cttttgatag gtattttctt gaccttcaac caaaccagta gcccaatgaa
    81361 cacagcgata tccagccagt agatgacatc atggggcttc atcaaagaaa aggagcttcc
    81421 tgacaatcct tgcgacactt tcgaaaagcc taatacagat ttgactgtaa tgaaatctgt
    81481 gaattcccga taaaaaataa tattcaagta caacaaaagc gtatttaaaa tatcaatgat
    81541 caaaagaata gctaacgctg gctttggctt tttaatatac aaagaaaggc caaaaaacaa
    81601 taaggtagtc gcaattgggt tgatccacaa gataaagtac tggatcgtac cttccacacc
    81661 taatgaaaaa tctgcatagt aggcaatgat cgtcttcagc cagaaaaaga aaacagctgt
    81721 caaaacgatt gctaaacgat tttggcacaa ttttttaagt tgtgtcacgt atatcacttc
    81781 tctctattca cattttttct tattttgctc ctgaaaaatg aaaatttcct aaaaaagttg
    81841 ttatcttaag atttttttat tctttctaaa gattattccg tccacttttc acagcataaa
    81901 accgctgcga tgtaagcgat taaactaatg aagagataaa agtcttgtgg tgaccgcttc
    81961 actccacggt attcaaaagc cagtttctcg gtattcgtac aaaaaaagag gctgtgacaa
    82021 aattcttgtc gcagctcctt atttcttgat gcaggacagc tttttcacaa cttcttcctt
    82081 aaacggtgtt ctcctgccaa ctcctcggcc acaagtcaca atatttataa aacatgagaa
    82141 gctgtttttt gaaattgttc cttgtggctc agagttaaac ggcaggctca agacctcttg
    82201 atacacggtg ttcaatcagc tgaccctcgg tattagatta tgtcaggacg ctaattttac
    82261 aacctcttgg taaacggtgt tcaaaagctt gcatctgcgg taataagccc aaccaaacga
    82321 aaaatataag gagctatttt ttgaaattgc tccttgtggc tcagagttaa acggcaggct
    82381 caagacctct tgatacacgg tgttcaatca gctgaccttc ggtattagct caaaaaacgg
    82441 caaaatatga ggaactattt tgccgtttgt ttctctaata ctcaggtcaa aacgctgatt
    82501 tcacaacctc tgtttttcat tgtccatagg attgatattg tccttgtgat gactcattcg
    82561 taataattga ggcttgattt tcttcacttc ccgttatcgt ttgtccactg ccagaatctg
    82621 aaggacttgt tcctgtcgac tcgttttttt gagaagagac agttgttttc ttgacttgtt
    82681 catttgtctt tttccattcc gctaaacgat cggcgaccac ttttttgtct actttttcag
    82741 aataagcggt gatcaaacgg gctactttgt cgccatcatg agtcaaaagc aaaacggtca
    82801 aatgcttgtc tttaaattga tattgtctga gagaaatcgc actaagtacg tcatcgatat
    82861 taataaattg attttcttct tttccttttg gcaaataaat gtccaattcg cctttttcgt
    82921 aattggcagt tattttatac tccgcccacc aataagttga gaattctttt ttgaaattat
    82981 ctactgattc tttgcttgct acgatttttt ctgtttcttt ctgagcaaga tctgctgtca
    83041 tttttcctga aaatgctgcg gataaaataa gcagtaagaa aaaaccgaat aaccaaaaga
    83101 gaagaaacgg tctttttttc ctcatgcttt cctccactcc tatttaattt tgactattct
    83161 attatataca aaaaatgaaa tagaaagaca aaaaagttga aaaagatcgt aaataattga
    83221 acttcttttt cacaaaatgg taagaatgga gaatttcacg catttgctct caactattct
    83281 ttcttaagcc tcaaatccgt ttttatctga tacgtctttg aaccaatgac ccgatttttt
    83341 gattgttctt tttccatctt gtgctaagtc tactgaaata aagccatatc gattcttata
    83401 tgcattggtc cacgaccagt tatccataca tgtccacata tgatagccta aacaattggc
    83461 accttcttga atggcttgat ggacatattt cagatgagat tggacaaatt cgatccgata
    83521 atcatcgtgg atcatgccat cttctttgat aaatcgtgct tcgttttcta cacccatacc
    83581 gttttcagaa ataaagcagt cgatatttcc ataatgatct cgtacattga tcaaaatatc
    83641 atagattcct tgttcataga tctcccatcc gcgataagga ttcattttac gaccaggcat
    83701 ttcataattg tcgaaataat cctcaggcat tggaccgttt ttagtatcga ttggtgtttc
    83761 ttttgctttt acccggcgtg gctggtaata attgatacct aaaagatcta ctgtattttc
    83821 tttgataacc gtcaaatcct ctgcatggat cacaggcatg agatcaagtt cttttactat
    83881 ctcaatcaat tcacttggaa actcgccttt tacagctgga tcaaggaatg aacgattgaa
    83941 gaacgcatct gctatttgcg ctgctttaac atcttcaggg tttgattcgt ctcttggata
    84001 gcttggtgtc agattcaaga tgataccaat aacaccacct tgctccattt catggtaaga
    84061 tttgatggct aacgctgaag ccaacgcttc atgatagcca acttggatcg cttttttcat
    84121 atctgactct tctggataat gccatcgata taaataacct gcttcgactg gaacgattgg
    84181 ttcattatga gtaaaccatt tttttacttt cgaaccaaac aattcaaaac aacttttggc
    84241 atatttttca taggcttcaa ccgtttctcg gttcaaccat ccgccttttt cttgcagata
    84301 catcggcata tcgaaatgat acagattcac aaagggttcg ataccgtttt caatcaactc
    84361 atcaaaataa cgatgataga aacgaacggc ttcttcgttg attgttccag attcatcagg
    84421 tatcaaacgg ctccattgga tagatgttcg aaatgaatta tgcccagtct gtttcatcaa
    84481 ctgtacatcc tcttgatact tcgtataaac ttgcgaggtt ttttcaggtc ctacttgatc
    84541 aaagaacttt tgcggttctt ttttgtacca ataatcaaaa atgttctctc cttttccatc
    84601 gcctgaaatc cttccttctg tttgtgggcc acttgctgca gaaccccacc aaaaatttgc
    84661 tggaaattga tattttttca tcaaagcacc tctttttctt ttttagttcg ttttagttga
    84721 ttccatgtat gatattttcc atcacaatca ctttttcatt ttcttcctct tcttttttga
    84781 ttcgttttaa cagctgttct gctgctcgtt ccccgatttt atctgttggt ggcgtgatcg
    84841 tgattttcgg aaggatactc agatcgtttt tatctaaata atcatagtga ctgaacgcaa
    84901 tatccttccc gattctgaca ccttcttgat tgaaacattc gatggcacca atcgacatat
    84961 tgtaattgct gacaaaaata gcagttgccc ctttttctaa taatgctttt gccccgtaat
    85021 agccgctttt tctagaatag tctcctgtaa agacctctaa ttggatcgat ggatcatttt
    85081 ggaccgcttc tttggctcct gctaaacgtt ctcttgctac gtagtctgtt tgagtggcag
    85141 caataaatcc gattttttta tgtccttgtt caccatgatg agcgatcact tttgctacac
    85201 tttctcgatc attcactaat attgtatcaa tacctgctgg tccttctgga caattcaatg
    85261 cgataactgg aatactcatt tttgtgagca tttcaaatcc agaccactct tcttcggata
    85321 agaagacaat cagcccatcg atcacatgct tcatcaggta ttcaattttt tgttcaattt
    85381 tttctggctg atcggcaaac ccgcttaata ataagctata cccattttct tcagctactc
    85441 tttcgatccc tgagacaact tccgacccaa aacggctgga aatcccgttt atcaaaagtc
    85501 caatagagaa gcttcgatta ttcttcaacc ctttagcaat gatgttttct ttatagtcca
    85561 acgctgaaat cgcttctgct atttttttca tgttcttttc cttcagttgc tgaccattca
    85621 agtaacggga aactgttccc actgaaacac cagccatctt agccacatca cggatcgatg
    85681 ccatacattc tcactcctgc cagttacatt tgatttctct ttcttgttgt tcgattatga
    85741 tgaccgcatt ctttgcatag tgttgcttgt catcaatatc catataacta aagtcttcag
    85801 tagagcaact tcctctcact attaattata cttaaacaag tatttgaacc ggttcatttt
    85861 taatatagca tttttttaaa agattgcaac acttttttaa aacgctttct gaaagtggcg
    85921 gattttttgc ctttttaaaa ttttattcta aaatttactc tatttgtggg tgttttgtgc
    85981 gtttttctct tccatccgtt tgaagtttta cattccaatg gtataatttc tttatcctat
    86041 caaatggggg aagtattttg aagaaattaa tcgtactatt tattttgtct gcgggacttt
    86101 tgacattgac tggctgtgat ttgtttggcg gctctgacca atcctcggac gaaacaaccg
    86161 attcttctgt cttaaaagcg gaaaaagcag ataccaccaa cagtgtatat acgctaaaag
    86221 tacgtaaaga tatggattta tccgcgggac agttgacatt tacagataaa aaaatggaat
    86281 ggacacgcac gtatcagaca caaggaactg attcttctga atcatctgaa gaaaaaacag
    86341 tgctgacaga tatcaagatc actaccaaag atgacgaata tattatttca ggaaaagaaa
    86401 ataaaaaaga aacaacagtc atatttacaa aaatcggtaa ttaccgtttg aaggatgagg
    86461 atggcaacat ctacagtcta tagtgccgtc tgttggatct tcttttgttt ctccccttct
    86521 tttctgctat agtaaagaag caagatgatt gtaaaggaga cgaaatggat gatcgatagt
    86581 cctgattatt gtacatgtaa taacaacaaa aatatccata cagaatttaa tgaccaagga
    86641 agcttgagca tatgcgacaa ctgtcacaaa ctagtcaaag attcatttaa aacatttgaa
    86701 tcttatcctg ttcaaaatta tgtaaaaagt agaagaagat aaacaaatcc aagcaaaact
    86761 cactatggct gatttttatg aacttcaaac tcagcttctt cctttgatca agcttcatca
    86821 gtaaaacaaa cagtggatca tttcagttta caaatcaaag agtaaagatt tatttatttc
    86881 aaatatttga atataaaata accaaacgat agaaaggctt attctacatc acgtagtaat
    86941 ttttctttcc cgtcgtttgg ttttttattt ttagcctaat tcttgacctt ttgcatgctg
    87001 gttatccgta aaatattttc cagcaggctt tccttccaat tcagctaatt tttcttggat
    87061 ctgacggatc accttttttg catcttctgg attttctaca aaatcgaatt tatccccatc
    87121 cacttgtatt ttaggacata catcaaaatc ttcataccat tgattatagc gggtgttcaa
    87181 cgtttcataa taggtatata acgtctcatc gctttctagc tgttcatagt cacgcccacg
    87241 ttttttgata cgttctagca tcgtgtccaa agaaactttg atatggacca tcaaatcagg
    87301 ccgttttttt ggagcaacat catctaactc gtttagcatc gtatctaata agctatcgta
    87361 ctgctgtact tcgatatccg ttacccgacc taaatcagcg tttagatgga acaacaggct
    87421 atcttcatag atggatcggt ctagcacatt gtcatcgttc actaaagcat ttttcattgc
    87481 taaaaatcgc ttgttcaaaa agaagatttg caataaaaaa gcgtactgct caggatttga
    87541 ataaaagagc ggcaatactt cgttatcttc gacattttca taaaatggtt tagaatcaat
    87601 ctcttttgca agcatatccg tcaaactgct cttacctgca ccaatcgttc ctgaaatcaa
    87661 aatcactgac atacaaccct tcctcttcta taagtactcg cctacgtaag tttcaacagg
    87721 caatgtttca aaatcatatt cacgtacagc ttggtctgat tttaaatacg tatggagact
    87781 ttgtgtaatc atcaagttcg taggtaccgt ctcttcttgt acaatgatca tcggtttttt
    87841 caacatagtt gccacaccta attcgtaagc cgttcctgga tcgattgttt gatgctcaaa
    87901 gtcaaggata gcaacaacaa attctgcatt tgtcagttct tccatatctt tttcgtatac
    87961 ttcctgcgcc catccagcgc tgaatagttc ataattgctt tcttggtgat gacgtggaga
    88021 gtagaaactt gtgactgttt tgttttcctc caacgctttt tcgatacgac ttactcgatc
    88081 gatttgctcc tctgaaaaaa atggtcctgc taaatatatt tgactcatta ttttgcctcc
    88141 ttcaataata actgattttt aatagatact gctcgtaatt cattgaccag ttccaatgga
    88201 ttgttggaaa agattacttg atccaaccat tttttatcca taaaatcctc ttttgcaaaa
    88261 gtttctaata attgttgcaa gccgctgtaa taaccgccaa tattcaatat tccgattggc
    88321 ttgtcgggcg tcagaccgat tgctatccaa gaaagaagtt gtgagaattc ctccaatgta
    88381 cctagacctc cagggaaaac gaggacagca tctccctttt caaataataa tacctttctt
    88441 tcatcgatcg tttctgtagg aataaatgta gtaacatctt ttccaggcaa ttcagtttca
    88501 aaaagcccat tcggatagat acctgtgact tttccgttat tttccagtac tgttttacta
    88561 atcactccca taatcccgga gtatgaacca ccgtaaacca gttcgatttc ttcttgtgcc
    88621 atgtattttc ctaacgtttg cgcaataaat ttgtaacttt ctttattacc aaaacgggaa
    88681 ccgcaaaaaa cagtaagttt cataaaaaaa cactccctaa ctcaaaagtc tatcttcatt
    88741 ctacacgtgt ttttttaaaa taaaagacta gattttgtag ttttttagtg gtatatagga
    88801 tagacgccac tatatatagt atttagaaaa aaatcatttt tcagccattt tatagataaa
    88861 acaaaaataa ataacgtgtt ttcatcgata tgaaatgact tacttataga agatactggg
    88921 atttcgtcct atatgccaat aacgtgtatt attttaaaca acaaatactt gtttctttta
    88981 ttaacgaaaa aatcattttg ttctcgtgtt atgtttcact gataaatgat gtttctctaa
    89041 ataaaaaagc cgacgaacta aacgatcaaa atttgatcat ttttgttcat cagcttctcc
    89101 taaaaatctt tttgttaacg ataaatagct ggatttgtaa aaatatcact tgaatcgtca
    89161 ctgctggaag aaaattcagt gataaccgca cctgttgcgc ctgcctggaa ctagtgtttg
    89221 atattgggct cgaacgtgta ctgatctcta gcatgaagaa taatttcttt ttttactgta
    89281 taataacttt ctttcctttt tggaatagta caggaaagtt tttttgtttc ctttccttcg
    89341 acataaggta taccagaccc tttcgacaac gaaaggttct catttttcct tcatcgtcat
    89401 ttcaaggagg gtgccgatgc tctggacagt ttcgatcgct cctaaaccaa aatctgtata
    89461 ttcgattctt tctaactctg attctttcat accattcctg tctcaaggta catctattgt
    89521 acttttttct tgtaatccat tctgcctctc ctaactaatt gtcaaatcat cccaagatat
    89581 gtcttcttcc tctttttctt cttggatgat ttctgcattc ggatctactg gttttttcaa
    89641 taataaaatg gttattcctg tgacaaaaga accagctaac aatgcaccaa tgaatgccca
    89701 tggtctggtc atcgtagcga ctgcaaacat tcccgatgcc ggaatacggc tatctgcgcc
    89761 ccaaaacatg cttaatccgc cacctactgt cccaccgact cccgttgcta tgacattgcg
    89821 tgccaagtcg ttcaatacga tagggaaaca tccctctgtg atctgacaga tccccatagg
    89881 aaaagcggtc ttcaatgtat cgatttcttg acgattgagt atatttttac gaatgatttt
    89941 ccctaagaaa taagcgatcg tcaagccgaa tggggttacc atcgaagcta aaatcaatac
    90001 agtgattggt tcatagattc cttctgaaaa tcgtgccaca cccccttatc cagaataaat
    90061 ggtaatccag aaacagccgt gtatctgcgg taataagcct aaccaaacga aaaatatgag
    90121 aagctatttt tgtttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct
    90181 ttttttattt atgtttcata atcaatatgg taaggatgca gctccgtatg gtagaccgcc
    90241 tccgaatatt caatcatttc cccaatttgc gtagacgatt tccgtatccg tttgatgccg
    90301 atctgtgcgt ctgttttcag aattccttgt tcttcttctg ttaaaaatac tgccgagaaa
    90361 tcatcagaaa atgagtcaat agtatatcct tttttcgtca gcaaacgata caaagattct
    90421 ttgccaaatt cttctctgct gaattgcatc atttcttgtg gaagataatg aacaaaataa
    90481 atataaggct gattgttcaa ataatataag cgcttaaaac aaacagcttg ctcaccgaaa
    90541 aaggaaaaca aaggatcttc cttctttata tggaccaatt gcaaatccag cgtttctttc
    90601 cggacttcat atcgggagtt ctccaagatt tgcgtaaagc ttgccgcttt ggataatcga
    90661 ttgtaaggac gattgcttag aaccgtcgtc ccacgaccac ttttctttgc gacatattca
    90721 tctgatgcca gcagttcgat tgcctttcgg acagtgattt tgctgacttg aaacagttct
    90781 tctaattcac tttctgtcgg caacatcgaa ccgactggat attttcctga gaaaatatct
    90841 tctttaattt tatctgcgac ttccaagtat agtacacgtg tccttttcat aacgtcaccc
    90901 tttgctttac tattactaaa agaatagcac ataaaaaata aaaaaagaac acaaatcaca
    90961 tgtgagaagt gtgttctttt tcattcagct gctttaatta caagtttacg ccatttgagg
    91021 caattacttc tttataccaa tcaaaagatt ttttctttga tcgattcagt gtcccatttc
    91081 cttcatcgtc tagatccaca tagataaagc cgtaacgttt actcatttct cctgttgatg
    91141 cactgacaag atcgatacat ccccaaggcg tatagcccat caaatctaca ccgtctgcta
    91201 ctgcattagc catcgcttcg atatgacgac gtaaataatc gatccgataa tcatcaataa
    91261 tcgtgccatc ctcttccata tgatcaatgg ctccaagacc gttttcaaca atgaataatg
    91321 gtttctggta acgattgtat aattcattca atgcgattct cagcccttct ggatcgatct
    91381 gccagcccca ttcacttgcc tcaagaaacg gattttttac tcctcccagc aagttccctt
    91441 ctgaagttgc tgcttgtgga tcagtctcat tgattgccgt agacatataa taactaaatc
    91501 cgatgtagtc cactggatat tcttttagca actggaagtc tttttccgtt tgctctaatt
    91561 tttccggatc gactccatat tttttatggg tacgcgctgt gtatgcaggg tattctccac
    91621 gtacttggac atccgtgcag aaaaaattga attcttggtt ttggatcatt gttgctgcct
    91681 gattgactgg attggcatcg atgctgtaag ttgttgcata gataatcatg cagccgattt
    91741 ggatatccga acgcaattcg tggccgattt ttacggcttt gctacttgca acgaattgat
    91801 tatgccatgc ttggaaaata ttttggtact ctcctgctcc attgcttttt actaatcctt
    91861 gacttaaagc tggaaagtga aaggccgaat tgatttcatt gaaagtcatc caatatttga
    91921 ctttactgct gtatctctct aatactgtct gagcaaatcg ttcatagaag tcgatcagtt
    91981 ttcgattttt ccagccaccg tattcttttg ccaaatgaag aggcatttcg taatgagaaa
    92041 tcgtgatcac aggttcaata tcatatttta agcactcatc aatcagctga tcataaaatt
    92101 ctaatcccgc ttcgttagga gtaccttcgt ctccatttgg aaaaattcgt gtccaggcaa
    92161 tcgaaaaacg ataacatttg aatcccattt ccgcaaacaa ggcaagatct tctttgaaac
    92221 gatcgtaatg atcgattcct cgatgattcg ggtaaatata tttttcttga tcaatcgtcc
    92281 agtcaaattc ttcactgccg ataatcgcaa agcgctgctt acctccaggc atagcatctg
    92341 caacggatag tccttttccg tctcgatcat aagccccttc taattgattt ggcagcagtt
    92401 gctccacccc ataaaaattt ttctgaaaaa cgtgattgca tcctgatttc ctccttattt
    92461 tacaattgcc agaacatctt cgtttgcaat aatctctttt tggtctaatt caagtatatc
    92521 ggcaaatgca tcagtattcg taatgacaac tggagtgatt acgctatagc cagctgctct
    92581 aattgcttcg atgtcaaaac gaatcaataa atctcctgct ttgacgagat ccccttgttt
    92641 gactaagatc tcaaagccat tcccatttaa ttctactgta tccatcccga tatgaatcaa
    92701 aatctccgct ccgtcagtcg ttgtgatccc tacagcatgt ccagttggaa aaatagtcgt
    92761 gatctctccg tctgcaggtg caactagttc tcctacttct ggatcaatag cgataccttt
    92821 tcccatcacg ccagaagaaa aaacttggtc tggtacttca ctcaatggca gtactttacc
    92881 cgttaaagga ctttttaagg tatgccgtgc tgaagtaatc ggttctcctg cattcgcatg
    92941 tttgttttct aattgattgt cttgtgtttg ttcgtcaaat cctaaaatca atgtacctac
    93001 gaatgcaaga acaaaagcaa ttcctgtagc tattactgct actgtcacat ttgactctac
    93061 accatccacc gtattaatga aagtcggaat cgtcaataag ctgaccaaac tgctggagaa
    93121 agcttttact ccggaaaatc cgataatagc tccaccaata cctccagaaa tacaagcggc
    93181 aatgaatggt ttcttcaaag gtaatgtcac accatacaca gttggttcag tgattccaaa
    93241 taacgaagtc actgtagaag aaatccctaa tgctcgaagt ttcgtatctt ttgttcgcaa
    93301 tgctacagct aaagcagccc caccttgtgc caagatggct ggaagaagca ttggcatcaa
    93361 tacatcaaac ccatattgtt cgatattcaa aaacatgatc ggtacgaatc cccgatgcat
    93421 gccgaacatc acgaatactt gccataaact tcccatgatt agacctgcta cgattggact
    93481 aaatccataa atcgagttga agagaccgcc taaagcatta ccggcaaccg tcccgattgg
    93541 tcctaaagca agaaatgtta atggaaccat cactactaaa acgacaagcg gaactaaaat
    93601 catttgcaga aattgcggaa tcactttttt tacgaatggc tctaatttac tttgaaccca
    93661 tacggctaga atgattggta tgacagaaga agtataacca gatggtgaaa gaatcactgg
    93721 gatcccaaag aaactgatag cttctcccgc accagcaact gctgtgatag ctggatacac
    93781 aagtgccata gcaattgcta ctgctaaaaa ctggttggct ttgaactttt tagctgctgt
    93841 aaaagcaagc atgacaggta agaaggtaaa gacaccatct gcttcagcaa acaatatttg
    93901 ataagtaccg ctatcagctg tcagccagcc catcaccgtt gccaatgata agaatccttt
    93961 caataccccg gcggctgcca tcgcccctag aaaaggtgta aatatactag agataatatc
    94021 gatcagacga ttcaaaatat ttttggactc ttcttttcca tcctccgaaa ctgcaagacc
    94081 ttcctcatcc acgatcgatt gataaacatc tgctacatta ctgccaatca ctacttgata
    94141 ctgaccgcca ctttgtacga cttggatcac gtccgggtct tcttttaact tttgcgtatc
    94201 tgctaaagat tcatctttta atctgaatcg caaccgcgta gcacaatgta ctaaactttt
    94261 gacattcttt tgtcctccta ccgcttccca tacacgacga ccaactgctt gattatccat
    94321 tgttttttcc ttctttcttt tttatttaaa aaataaaaac ccagattgaa aaaaacagtc
    94381 acaaacattc gtgaacctat tttttcaatc caggttttgc ctgccataca gtaacaatcc
    94441 ttatgattgg atgctttttt tctcacttac tactctttga atatgaatcg ttaaataaat
    94501 ctgctctgcg atccccattt cctgtttttg cgtttgtcga agatactcat tgatccgttc
    94561 gacacaatga aacgcttttg gatactttcc ttgaactaat gcgtagagaa attcatcttg
    94621 ctcgtcagat ccttcccctt tgagataacg ctgggcaaaa aactgcagat gagtaatgat
    94681 acgctgataa ttcaaagatt cttcatcgaa cacgatgccg aagaatttac tgatgatact
    94741 cagtatatct cttaccattg tggtagcggt catcgtcact tgcatgtctt cgtttccttg
    94801 ttggctattc acaaaatgaa aggcaataaa accggcttca tcctcagaaa gttccacacc
    94861 cgtcttttgt ttcacgaaca aaagagcctc tttcgctgct tgatattctt tcggataaaa
    94921 tttttttgtt tcaaataata atggattagg cagttggata ttttcttttg ctcgtgtgat
    94981 cgcataatga agatgatcgg tcaatgtcag ataaatatta ggcgaaaatt caacttttag
    95041 tttatcttca gccagttcga ctacttgttt gacccattcg atctcctctg ccggaatatc
    95101 agccatcagc tgctggaatt ggtttttagc aaaagaatct cttaaaacaa attctttttg
    95161 gaccgcttct ggcgaaatct cgtccccaat ctttttttga aaagctagtc cttttcccat
    95221 acaaacgact tcttccccga atccattttt cgaaatgacg acattgttat tcaaaatttt
    95281 atcaattttc attttgcttc gccctctcaa gaaaaataaa aaaacctaga attcgcctac
    95341 tgtttcttag ctgaattctg gttttgcctg ccgtacagta acaatccatt tcttattcaa
    95401 aatcatcata ccatcgaatt tttatttatg caagcctttt catataaaaa ataaaaaaac
    95461 tggaacacaa gtctatctag aaaacaacca aatcagagat gcacctcttt gcgtttctct
    95521 ttttttcagc aaattggttt ttccttatcg attcatatcc tttggctgtt tttaatctgt
    95581 aacgacttat gtcccagtat cctaaaatgt ttttgtctct agattgaaag cagatctcat
    95641 atcatctgtc gcatctttta tcgatgtaac actttccagt tctccttgta caactagtcg
    95701 cgtgacggcg tccatgtcgc cacaagtaat tagtgtgaca atttcttttc cctcgacttc
    95761 attcagtaat tgggtatctg taggagcaac tttttcttta aaaaatgttt tgtatgtata
    95821 cacattttct aaatctgtca aatagatctt ggcacccatc tctaaatttt ctagcggtgt
    95881 aaacagcaat tcagggttcg ttgcacgatg gcttgctaag gcataatttc cttttcccat
    95941 ttcttgatct ggagataatg ttcctgctcc gtaaagtagt gacgtattat ccaagccttt
    96001 gaaaaccggt aaattgattg aaactgaagg gactgcgata ctagcaatca ctggtagatc
    96061 tgttcctctc atttgcgacc tcatgacccc ttctgtcgtc attggttcta ccgcatcaaa
    96121 gtcaaaactg acatctttat cgttattctt tttcagatct tcttttgtta cattcgctat
    96181 agcatattta tctcctgtct ctctgacaaa ataatccttg atctgttcat tgaaaatcaa
    96241 agctagtcct actaataaca agaggaacaa gaggatattg actagccatc tttttgcttt
    96301 tccttttttt ttcattttct ctccctccag actcatttta ctgtttttct tttgctcttt
    96361 ccaacagccg aatgacacat ttctacaact ctgtgtattt ttttcgtaca cctgcgcttt
    96421 tttctacagg atatttcaaa ttatttttga tcaatttttc ttcaatgatc tcatctagat
    96481 ccaggtgaag gttgtctgcc atcatgtatg aatagatcaa tacatctgcc agttcttctt
    96541 tgattcgttc taattgtgtt tcttttactt cttccggcgt tttccattga aaaagttcta
    96601 acaattcaga tgcttccaaa gaaatagaga tagccaaatc tttttcgtta tgaaattgtc
    96661 gccaattacg ttcatctcta aactggttga ctttttccat actattcatt tgcttgcctt
    96721 cttccaatca cttcttactt gattaatcat accatttttt tacataaaaa atagatgatg
    96781 cttttaaaaa aactcctgtt ttcttttatt taaaataaat gtcatagaaa atcaataatt
    96841 taggtagcca ccatgtgtat gcagtagcgt gtcttcttcc cttgaggctt tttgtttttc
    96901 gatacgttct tcttcccaaa cagacaacgc tactagaatc tctcttaatg attttccttc
    96961 ttcggtgagc gagtactcta ctttaggcgg gacttggtta taggcttttc gatggatact
    97021 ttcatcttgt tctaactctc ttaactgctg ggtcagcatt ttttttgtga tcgtagggat
    97081 ttgcttgctt ctccacgcct aaaataagcg atattgctct tccctttatc tggtgtaagc
    97141 aaagcagtta gcattttgac gatcgtgggt tccgcttttc acttcttatc tacttagtat
    97201 catgaagtat actttcgtac taatactaag tagcggaagt gtcaactact ttgtttaact
    97261 acatagcaaa agaaacagaa gggtgacaac ggtcatcacg cttctgtttc ttttgaatat
    97321 gctgtgttgt tgtctctatc tccgtgtaga ctttcctcaa acttatcctt tgacagtaag
    97381 tcagtaggct gatttgctct ttctagatgc cagatacatt tctacaaaaa aatagtgatt
    97441 ctgttttgaa aattcgattt ttagttctgc attttcgtaa acatccaaca tttttttcgc
    97501 actgtataga cctaacccgc catttttttt agaggttttt cctcttttga ccatctccga
    97561 tagcgtcaaa ttcgttggat ttgccttgtt cgctatacta aaacgtgatc cctctttttc
    97621 ttccagcaga ttgatcgtaa tcggactctg atcggctaca cgatgatcga atgcgttaga
    97681 aataaaaata gataaacagc gaataaaaac aaccagatca atcgggattg ttttcacttc
    97741 gacaggaata actacttgga tcgggatttt ctccgattct gctttttcta gaaattttaa
    97801 aaagacaccc ctgacagctg gggatacaat atttgttagc tgttgtattt gcggtttttc
    97861 aaaaatagct gcagaatgat cttctaatat ttctaaaaat tccaacgttt cttttttatt
    97921 ttcctgaaga gtaattttta atgaaaacag tagactttta taatcatgtt tgaattcctc
    97981 tagatgatgg taatattctt catttatttg atacagttga agcgattcct gatgttgtct
    98041 ttcttttact tggaaactga tcaacataat ggaaaaaaac aaagaaaaaa aactgatgac
    98101 tatcgacaaa agagatagaa gcagataatt agatgagcgg aagtgaacac ttcccaatac
    98161 caagtaaatc acgccccagc ttaataaaat acaaatacct ccaaaatagt gttcagttgg
    98221 ataagtaaaa agcaagttga gaaatctcgt ctgtttgtct atacttttta ctaatagata
    98281 gaaaataaat gcgataagta gtgcaaaaaa acttgctgtt ccaagatgtc tctttcctaa
    98341 ataatccatc agatcaaaaa ccagaaaaaa gctgagtcca gtgatagcaa aaaaaagatt
    98401 actgatcaaa cagctgataa acaggttttt cgtagtaaaa taaaaaagaa acatcaataa
    98461 taagccaact aggtaaggaa agctcacttt agggatagga agtaaaagtg tcaccaccat
    98521 caaagctata caaccaaagc tgttttttac tagcagttgt ttcgaaaaga ataaatagat
    98581 acctaaatct aaaatcaaag taaaggtcaa ataaagtgtc actgttgcga ccatcttatt
    98641 cctccattgt tttgtttcaa aatgtacaca tattttttta ataggagcct gcatatgaaa
    98701 aagtatttta tcatagaaga taaccagttt cacagcgaat ggatcatcag agaattggtc
    98761 aatcatcata tcgagaatat cgtgcagctc acttctatct ctcaaatcaa tcagttgatc
    98821 cagttgggca ctattgataa tcacgatgtt ttctttatcg atatcgactt gaagcaatca
    98881 ctcacaggta ttgatgtcgc cgaacaaata cgtaaaaaaa atgcacatag taatattgtt
    98941 tttttcaccg cttatacgaa ttttgcagta gagatcatca atcggaaaat ccttccgatt
    99001 ggatatatca gaaaagacag gaatttatct gaacaaattc gtcaagtgct agaacagatc
    99061 aattggaaag aacagcagat cgtaaatgtc gataaaatct ttattgatca aaaaggtgga
    99121 agtctcgtcc tgtatcctcg ggaaatttta tatattgaaa gtatcaaagg tttgaaaaac
    99181 caagttatgg tcaaaacaac tacagaagat aaattagtga atacttcttt aaaacaattg
    99241 aagacagagt tagaaaagta tgcttacttt ttacttttaa aatcttactg tatcaactta
    99301 tcgcaattgc agaaaattga ttctgctcat tatgtgttag aatttttcaa cggtgactcc
    99361 ttactcgttg gtcgaaagat ctttgaaaag acaaaggaac gtttccatga cttccaaaaa
    99421 actgcttcga gttgatgaat aagagctgag acgtaagcat agcagatttc agggagtggg
    99481 ccttgaatcg gctccactcc ctgaaatcgt tgatatgatt ttacgcttcc tcttctgtag
    99541 gagcatctgt taacgaccat gtgaatgttg aaacgtagtt gtctacagca taattggata
    99601 caggaacaaa taattccact gattgtctcc cttgttccac attctctcct aatgaataaa
    99661 tccatgtgcc aatcccttca tttgctcctg cagttactag atctactgcc tgatctgctg
    99721 ggatttcgat tccttcagcg ccagttccag aaacggtaga tggcattcca ctgctattag
    99781 ttgcgctatt tcgaatcacg gcgtttttga atttcacctt tgaaccagtt agtttattcg
    99841 tatcagatgt tgtaccaata aattctgttc gtttgactga taatttccaa ccggcataat
    99901 ttccgctttt atccgttact tgtacgaaat tctgtacttg tgtttgtgct ccagtactgt
    99961 ccgttccgct ttgtaatgca gcatatactg tttttgctgt acctaacttg attgttccaa
   100021 aatcaagatc tggagcttgg tcaatcgtca gtaatccgga tgttccctga tttggttcgt
   100081 caggtgtaaa aggagtatca ggatcatttg gatcaacagg aggaactgga acaacgtctt
   100141 caggatttgc agcatctggt ttggtcacag aaatcgttcc ttctgtagtg gctgagctct
   100201 tattctcagc aaaaacactt tgtgtacata ataatagact tccagctaga acggtactta
   100261 atgcaattgt ttttttcatt tctttttcct tctttctttt aaaataaata atccagcaaa
   100321 taagccaata agaatgactc caacaagtat cagccatcgc cagtcaaatg tttctggctg
   100381 attttttaaa tcaatatcct tttcattcaa ttggtccgct gcatcatcag ttatttcaaa
   100441 atcggtatgg agattccagc gtttttcttt ccaagcaccc ttcacaaccg cttgataatg
   100501 tcccggagct aatttttttc cttctaatgg gacaagtaaa tcaaaatttg tattgggtgc
   100561 tacatctatt ttttcttttt ttattgaaag gtaaggctct tttgtttctt ttttgtagat
   100621 ttcaaccgat gtagtcaagc cgaacatata agaagcattt ggattttgaa gattcgcctt
   100681 aatcacattt cttccatcgc tttgatccgc ataagctttt tttaatttca aatctgggga
   100741 agactctttt tcgctatttc aaatcaagat tgctttcact tgagaaaact gattgacgac
   100801 accaactgta cttttgttag aaacgttctc ttttttttct tgaaacgtga tccctcctgc
   100861 gaccacacca gccagctctt tttctggcgc tttcagtgaa agtgtctcag tgaccgcact
   100921 ttgaggagga acagttattt ctcccactgt ttttgctaag tttttcatat cataacttaa
   100981 tgtttcatca aatttacttt ttgattctcc atattcgatc acaccgtttg aattcgtcgt
   101041 agcggagtgg atcaatgtct caactgttac tgatttatct gttcggttaa agaccgtgta
   101101 ggggatttcc tgtgttttcc caggctccat ccttacattt atatatgttt tgttcttatc
   101161 aatctgatta gccggtaatt caaaatctac cgcaaaattg aattcggagg catggacagg
   101221 tgtctgccaa aggatgaatc caaggagaag gatcataagt aaagagcagt tgtttcgatg
   101281 acttttattc catattcgat tcatcaatca tcctccttga tctttattct gtcggcgcat
   101341 ctgctaatgt ccatgttatc tctgctgagt aattaccagc tatctgattt tctttggtta
   101401 agacaagttt aaacgcccga ccgttattta aatattctga ataatctgct tgcacattcg
   101461 ttgaattttg tcctctgaca atttccagtt cttcttcatt cactaattga tactcctctc
   101521 ctttcttgaa atataggaca tcctgcatag aaaatcctgc atcatttgtg aaaggctgag
   101581 ataattttat cgttaagatc cacggatttt tcgtttctcg ctgatcataa atccataatt
   101641 tttcctgtat atccatttgg ccaaatgctt cttgtgtcaa accagttgct ttgatctgac
   101701 caaaatcgag tttggaaggg gcaagaaatg agagaccacc ttctactacg atcttcgtag
   101761 caggtgcaaa aggcggggca tcatgataaa caactggcgt atcacttgga ttgtgattcc
   101821 caatttcatc gttggtactt ggcttgttct gcacaccctt agcagtagct tctcctgctt
   101881 ggtcgtttaa tacaatttct aatacatctc ctgcaaaaag ttgattttct gggatagaaa
   101941 gaacaaattc ccctgtatca tcaatagttg tttctgacaa aacagcttga ttttttttca
   102001 atgaaaccaa ggctcctgct tcacctgtac ctgtgatcgt gcgagaagct gcattgatca
   102061 atgtggtact taaattagca ggttcaggag gaatcacatc gactgtcgtt ttggcagagt
   102121 ccagttctgt cacttcttgg ccacctctta cttttttagc agcaacaact tgcacttgat
   102181 cctccgtttt taatctctca tcattgggta cttgaacttc gaacaagccg ccttgttcgc
   102241 cttctcccca tgggttatat cctgcccctt cattttcatc tgtttgtcct tgcgtgagag
   102301 cagtaatttc tacagatgat ttcgtcgtat ctgcgtaagt aatacgtaaa gtcacttcta
   102361 cttcgcctgt ccaagcatct cttggctttg cctcgacacc ttcgggaaca acgacatgcc
   102421 catagatttt tttatctgca ttcgttggca ctcttaagga ttcaatcatc gggagctgat
   102481 tattggcact gattcgatta taatttttca ttccggcacc tgtctgagca aattcatttg
   102541 ttaaacctgc actagggctt gatacgaaat tgcctaagtc ggtcccattg atcgataagt
   102601 ctgtcatatc agaaaaataa tccgggtctt tttcaaagtt atcgaatgtt gcgcctgttt
   102661 ttcgccataa agcgatttct gaatgggcga tctttaaaga agaatctcca tttccttgga
   102721 atacccagcc gcctatgcca ttggtctcgc ttccaatccg tttgttttgg aaatcaaaat
   102781 aggtaggaga ttctaagtcc acattcaaca catccccact gatgatgccg gtcttatctt
   102841 cccctgtttt cccgaccatg actaaataag ttttttcacc tgccgtaaaa tttaaatcaa
   102901 tatctccagt tgtatctaca gttgctccgg aatctgcgcg tatatcgata tttgattttt
   102961 ctccagtgac tgtaaaatca gctgaacctg gactttctag aaaaccagaa gcgtcataaa
   103021 attggatcgc ttgatttcct tgattgagtc tgttcacgta agatttgcca tcacctgtat
   103081 ttcttaccct caatgagcct ccattggtta ctgtcagctt ttgcgtacca ttctcaaaac
   103141 gaactgcgct ggaacgccca ctctgcttat cgatgtcgat aactgatccg gctccttcta
   103201 cgatcaaaga aaggcctttt cctacaaaac gcatcccatt gttcccgggt tgatcgtcat
   103261 ctccagccat tttgaccgag atttgtccac cacttgatac atggatttga ctagaatctc
   103321 cttgcaagcg aaatccgccc gtagttgctg tattgatggt gattttccca ccattcgata
   103381 cagtgacttt cgatcctgaa cctctcattt ggatgattcc gcgattgttg tcattggtaa
   103441 tattgatctt tgtatttacc gaaaattcgc ttcccgaacc actgacttca aacatgacat
   103501 taccaggtag cgtatcgact gcctctcctt catcgctaac gccatctgcc cgatcataat
   103561 caagagagac ctttgaaccg cttaagatat ggattcctgg cgctgttttt ccactaccag
   103621 tctcatctcc tgaatctaga aaaccgtaga atttccctag atacggtcca taaagttgac
   103681 taccattgat cacattcaat tgatagcttc tacaaaaata ggctttaccc ggagcggcat
   103741 tcagtccttc cttcactggt tggacatcca acacaccgga aacaccatct aatgtgacag
   103801 ttgcacgagg agcgtaaatc agtcctaatt tggacccatc atacaaatca agtgtcccgg
   103861 taaattttat ttctcccgta aaatgatatc tgcttcccca aaaatcggtc atataagcaa
   103921 tgataatggc atttccatcg atatttggtg tcaatccacc tggatgtgca gctgttcctt
   103981 taaaattctg gaactcgatc gactgaggta tctcattgat ataaatatgg tgtgtgccga
   104041 aattgtgagc gaatccacca aaatcaataa cgatatctcg tttgatctct aattccctgt
   104101 ctaaagtcaa aggctgaatc agtcggatct tagttactga ttcgtctgcg agaagctgcg
   104161 ccagttcttc tgttgtggca gcttcgctgt acccatccgc tgaaacagct cttgccagtg
   104221 ccgcttcact gctatcagac gtttgttcag ctacttctga ggtctcactt gtcttatttt
   104281 cttcttgcct tttattagta gaagcctcac tttgagattc tgtagtttca gaagtaatcg
   104341 attcttctga aaaagtagtc ttgtcaaaac tttgattctc tgattgcgtt gattcgtttg
   104401 ttttctctac atcggcaaaa acggatgtca ccatcaagag agggaagaca aacaaaatcg
   104461 acgcaatcag cttttttaca cattgattca ttctccacca tcctctctga cgttttgaca
   104521 attttatttt atcgttttaa tctatttcca attgtacttt catatttaaa atatgtattt
   104581 ttatggaaaa atttttctat taggtgtaaa tttctttttt gtacaaaaaa aaggctgtga
   104641 caaaagtctt gtcacagtcc cttatcccga acaagcggtg ttctcccgcc aactcctcgg
   104701 tcacaacctc cattttaaca ataaacctgt aaatgtgagg gcaatacttt gattgtcaca
   104761 ggtaatttat ccccttcatc tccatcaaca ttcgtcgtca attcttcctc atcagctaat
   104821 gacacaacta attgctcaca agtaaggtag gctagatttt tcgtagattc atctactcct
   104881 tttaataggt ctggcaatgt tttaagcgta tcgatcaaag aagaatcttt taaataaacc
   104941 aaatggagct taccatcatc cacttttgca tttggaagta atgtttcaaa accgccgact
   105001 gagttagtta gtcctatcag caaggtactg ctctcaattt cttccttctt tccatcaact
   105061 tctacgtgaa acgagtagga ttgtgtggaa gctaactgtt tgattcctga catgaaataa
   105121 gctagttttc caaatttcgt cttcttctcc gcatctacat cattgattga ttcagggata
   105181 gagccaattg cgacgacatt cataaaatag tcgtcattta tttttccaat atccaatggc
   105241 ttgactgatt caatcgaaaa atgattgatc gcttcctcag gttctagcgg tatccctaat
   105301 gctcgagcca agtcattcac cgtacccaat gggaaaaatc cgaaatttgg ccgatgttct
   105361 tgttcagcga ttccactgat tccttcgttg actgttccat ctccgcccat gacaaacaca
   105421 ctatgatacc cttccgtagc cgcttcgcga gtaaagtttt ttgcatcccc tgcttttttt
   105481 gtatgcaaga cgaccacttc atcaaacacc gattccaatt ttgctatcgc taattgctca
   105541 aattcctttg cttgttcacc gcctgaactg gggtttacca ctaataaaac ttttttcatc
   105601 actttctttc ccttctgatt gatttcttta atataacgat agcataaaga aaaaaattag
   105661 gctgtgatga agtactgttc atgaaacatt catttatttt ttgacaagat aaaacatcca
   105721 tactatgaaa ctgaggaaaa ttaaaacaat gatataggaa ataaaagaaa atgataggca
   105781 ttgtatacct aataaagtaa aaatcttagg tggcagataa aaacacaaaa atagcaagag
   105841 aatgtaatag cgtttcttcc actttttgtt atcagacctt atagcgatca gaggaatacc
   105901 aaatgcataa ctgaaaaact cttgatgtac tgttaatatg tcacggcctt gaataataaa
   105961 tgcgacaatc cccgctataa ttagtgtgcc taacaccaaa acggaaaatt gatgaatata
   106021 ttttatatac aaatgatcca ttgtacttcc tgccttttta tttgtgagaa aaacaaatcc
   106081 gtatacgtga ataacttcta gaaataaaag aaatttgcaa aaccactcca tttatctact
   106141 tctacgtgca ttttttgata ttgttttctt tatttaagat tatacagaaa aatatctagg
   106201 gtcgctggat tctaagtaaa aaaaattttt aaaataaaaa gaactaaatc aagtagtaat
   106261 taaactactg ttgatttagt tctaataaaa agatttagat gaacagtttt attgatttaa
   106321 atagttcata ttttttattt ataccgctta tttactactt gctgtactag aatctgaagt
   106381 gaatgtactc aaaacatttt caaagtcttt gtctttgatt tttacattag cgtctttcaa
   106441 tacttcgcca attactttat tctggaaggt actgtcactt aattttgtgt ctgttgcaat
   106501 ttctttcaat tcttttttgt atttatccat gtcgttgcct ttgttttggt ttttcaccat
   106561 tttaaccaca taatattccg tagtataagt tgaagcattg gtagaagtga tcacatcaga
   106621 aacttgacca tctttcaact taaaggctgc ttctttgact tctgccggaa ccgttgtagt
   106681 agtggaatca aatttcactt ttccgccatc ttcttttgtc gtagtatcag ttgacttgtc
   106741 tttggctaat ttagagaaat cgtctccctt atcagcagct ttcttcacat cttttgcatc
   106801 atcttcactt gctacttgga taatttgagc ttccacttct ggatggaaag attcccaagc
   106861 cgtttttaaa tcttcatcgg tcaatttgat atgtttcttt agaccttctt gaaaggctag
   106921 actttgtttg atactgtctt tgaatgtttg ttctgtgtaa cctgcagatt tcaattgtga
   106981 atcaaatgaa tcacccaatt gtttctttgt ttgatcatat tgagaatcga tttctttggt
   107041 tgatacgtca tctccatatt tatcttcaaa tacttgataa acgatcaact gttgcacagc
   107101 ggaatctgct ccttgcattt ttttcaagct atccattact tcttgttcgc tgattgtacc
   107161 gcctttataa ctaacggctg ttttttcacc ggcattcgta cagcctgaca ataagccagt
   107221 gcctagtacg atggcaagtg ctaaaaatct ttttttcatt tatcaattct cctacttttc
   107281 gttttttgcg aattgctaaa aacaaaggtg ttcaagatga aaatcatcaa gaacaccttt
   107341 actaatctgc tcgatacgtc ctgaatagat ataaggctac acaatccaga aagttcgctc
   107401 agcagaataa atgtcaagta gcacatcaca ccttaatata ctacttcttt tgttaccgtt
   107461 tttcaagtct atcttgcgtt ccgtatttaa cgtaactaat tcttagaaca aatgttatcg
   107521 attttgtttt tcttcttccg ctgctgggat attcaaatca gacactttta tgttcgattt
   107581 tccagacatt tttttgttgt ttttattatg atatattttt ata
//
LOCUS       DAFEPQ010000002        98487 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.002, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000002
VERSION     DAFEPQ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 98487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 98487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..98487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.002"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            598..1287
                     /locus_tag="LP543_000098"
     CDS             598..1287
                     /locus_tag="LP543_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2320297.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1274..2482
                     /locus_tag="LP543_000099"
     CDS             1274..2482
                     /locus_tag="LP543_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL2320298.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2776..4047
                     /gene="serS"
                     /locus_tag="LP543_000100"
     CDS             2776..4047
                     /gene="serS"
                     /locus_tag="LP543_000100"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBL2320299.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="LP543_000101"
     CDS             complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="LP543_000101"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBL2320300.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6585..7280)
                     /locus_tag="LP543_000102"
     CDS             complement(6585..7280)
                     /locus_tag="LP543_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBL2320301.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLTPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="LP543_000103"
     CDS             complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="LP543_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBL2320302.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8479..8670)
                     /locus_tag="LP543_000104"
     CDS             complement(8479..8670)
                     /locus_tag="LP543_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBL2320303.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8691..9581)
                     /locus_tag="LP543_000105"
     CDS             complement(8691..9581)
                     /locus_tag="LP543_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBL2320304.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9568..10335)
                     /locus_tag="LP543_000106"
     CDS             complement(9568..10335)
                     /locus_tag="LP543_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL2320305.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="LP543_000107"
     CDS             complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="LP543_000107"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBL2320306.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="LP543_000108"
     CDS             complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="LP543_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBL2320307.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEVQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="LP543_000109"
     CDS             complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="LP543_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBL2320308.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14906..15289
                     /locus_tag="LP543_000110"
     CDS             14906..15289
                     /locus_tag="LP543_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320309.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15457..16215)
                     /locus_tag="LP543_000111"
     CDS             complement(15457..16215)
                     /locus_tag="LP543_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HBL2320310.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16233..17051)
                     /locus_tag="LP543_000112"
     CDS             complement(16233..17051)
                     /locus_tag="LP543_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBL2320311.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="LP543_000113"
     CDS             complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="LP543_000113"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBL2320312.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="LP543_000114"
     CDS             complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="LP543_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBL2320313.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18179..19513
                     /gene="dnaA"
                     /locus_tag="LP543_000115"
     CDS             18179..19513
                     /gene="dnaA"
                     /locus_tag="LP543_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBL2320314.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19711..20841
                     /gene="dnaN"
                     /locus_tag="LP543_000116"
     CDS             19711..20841
                     /gene="dnaN"
                     /locus_tag="LP543_000116"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBL2320315.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21070..21312
                     /gene="yaaA"
                     /locus_tag="LP543_000117"
     CDS             21070..21312
                     /gene="yaaA"
                     /locus_tag="LP543_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBL2320316.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21299..22423
                     /gene="recF"
                     /locus_tag="LP543_000118"
     CDS             21299..22423
                     /gene="recF"
                     /locus_tag="LP543_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBL2320317.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22420..24366
                     /gene="gyrB"
                     /locus_tag="LP543_000119"
     CDS             22420..24366
                     /gene="gyrB"
                     /locus_tag="LP543_000119"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBL2320318.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24386..26857
                     /gene="gyrA"
                     /locus_tag="LP543_000120"
     CDS             24386..26857
                     /gene="gyrA"
                     /locus_tag="LP543_000120"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBL2320319.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDRAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27093..27392
                     /locus_tag="LP543_000121"
     CDS             27093..27392
                     /locus_tag="LP543_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBL2320320.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27442..27954
                     /gene="ssb"
                     /locus_tag="LP543_000122"
     CDS             27442..27954
                     /gene="ssb"
                     /locus_tag="LP543_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL2320321.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27980..28216
                     /locus_tag="LP543_000123"
     CDS             27980..28216
                     /locus_tag="LP543_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBL2320322.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28359..30332
                     /locus_tag="LP543_000124"
     CDS             28359..30332
                     /locus_tag="LP543_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBL2320323.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30338..30790
                     /gene="rplI"
                     /locus_tag="LP543_000125"
     CDS             30338..30790
                     /gene="rplI"
                     /locus_tag="LP543_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBL2320324.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31064..32431
                     /gene="dnaB"
                     /locus_tag="LP543_000126"
     CDS             31064..32431
                     /gene="dnaB"
                     /locus_tag="LP543_000126"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBL2320325.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNIIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33211..34503
                     /locus_tag="LP543_000127"
     CDS             33211..34503
                     /locus_tag="LP543_000127"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBL2320326.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35290..36153
                     /locus_tag="LP543_000128"
     CDS             35290..36153
                     /locus_tag="LP543_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL2320327.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36282..37904)
                     /locus_tag="LP543_000129"
     CDS             complement(36282..37904)
                     /locus_tag="LP543_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2320328.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38153..38674
                     /locus_tag="LP543_000130"
     CDS             38153..38674
                     /locus_tag="LP543_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBL2320329.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38860..40590)
                     /gene="spxB"
                     /locus_tag="LP543_000131"
     CDS             complement(38860..40590)
                     /gene="spxB"
                     /locus_tag="LP543_000131"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBL2320330.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40755..41573
                     /locus_tag="LP543_000132"
     CDS             40755..41573
                     /locus_tag="LP543_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBL2320331.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41676..41873)
                     /locus_tag="LP543_000133"
     CDS             complement(41676..41873)
                     /locus_tag="LP543_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320332.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            42042..42620
                     /locus_tag="LP543_000134"
     CDS             42042..42620
                     /locus_tag="LP543_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBL2320333.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            42715..42852
                     /locus_tag="LP543_000135"
     CDS             42715..42852
                     /locus_tag="LP543_000135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320334.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(43665..44168)
                     /locus_tag="LP543_000136"
     CDS             complement(43665..44168)
                     /locus_tag="LP543_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL2320335.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44526..45551
                     /locus_tag="LP543_000137"
     CDS             44526..45551
                     /locus_tag="LP543_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL2320336.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45874..46752
                     /locus_tag="LP543_000138"
     CDS             45874..46752
                     /locus_tag="LP543_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HBL2320337.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            47228..47344
                     /locus_tag="LP543_000139"
     CDS             47228..47344
                     /locus_tag="LP543_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBL2320338.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            48272..48610
                     /locus_tag="LP543_000140"
     CDS             48272..48610
                     /locus_tag="LP543_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320339.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48704..48934
                     /locus_tag="LP543_000141"
     CDS             48704..48934
                     /locus_tag="LP543_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320340.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            49334..49705
                     /locus_tag="LP543_000142"
     CDS             49334..49705
                     /locus_tag="LP543_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBL2320341.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(49979..50671)
                     /locus_tag="LP543_000143"
     CDS             complement(49979..50671)
                     /locus_tag="LP543_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL2320342.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(50674..51459)
                     /locus_tag="LP543_000144"
     CDS             complement(50674..51459)
                     /locus_tag="LP543_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL2320343.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            51696..53096
                     /gene="lacG"
                     /locus_tag="LP543_000145"
     CDS             51696..53096
                     /gene="lacG"
                     /locus_tag="LP543_000145"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBL2320344.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            53121..53438
                     /locus_tag="LP543_000146"
     CDS             53121..53438
                     /locus_tag="LP543_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL2320345.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            53451..55124
                     /locus_tag="LP543_000147"
     CDS             53451..55124
                     /locus_tag="LP543_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBL2320346.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            55138..56769
                     /locus_tag="LP543_000148"
     CDS             55138..56769
                     /locus_tag="LP543_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HBL2320347.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(57260..58825)
                     /gene="guaA"
                     /locus_tag="LP543_000149"
     CDS             complement(57260..58825)
                     /gene="guaA"
                     /locus_tag="LP543_000149"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBL2320348.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(58884..59807)
                     /locus_tag="LP543_000150"
     CDS             complement(58884..59807)
                     /locus_tag="LP543_000150"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBL2320349.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            60003..60608
                     /locus_tag="LP543_000151"
     CDS             60003..60608
                     /locus_tag="LP543_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL2320350.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            60916..61932
                     /locus_tag="LP543_000152"
     CDS             60916..61932
                     /locus_tag="LP543_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBL2320351.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            62073..63374
                     /locus_tag="LP543_000153"
     CDS             62073..63374
                     /locus_tag="LP543_000153"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBL2320352.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            63420..64082
                     /gene="deoC"
                     /locus_tag="LP543_000154"
     CDS             63420..64082
                     /gene="deoC"
                     /locus_tag="LP543_000154"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBL2320353.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIVETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            64103..64501
                     /locus_tag="LP543_000155"
     CDS             64103..64501
                     /locus_tag="LP543_000155"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBL2320354.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            64567..65649
                     /locus_tag="LP543_000156"
     CDS             64567..65649
                     /locus_tag="LP543_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL2320355.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            65861..67423
                     /locus_tag="LP543_000157"
     CDS             65861..67423
                     /locus_tag="LP543_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2320356.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            67416..68549
                     /locus_tag="LP543_000158"
     CDS             67416..68549
                     /locus_tag="LP543_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2320357.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            68549..69505
                     /locus_tag="LP543_000159"
     CDS             68549..69505
                     /locus_tag="LP543_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2320358.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            69604..70770
                     /gene="deoB"
                     /locus_tag="LP543_000160"
     CDS             69604..70770
                     /gene="deoB"
                     /locus_tag="LP543_000160"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBL2320359.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            70794..71612
                     /locus_tag="LP543_000161"
     CDS             70794..71612
                     /locus_tag="LP543_000161"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBL2320360.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            71629..72336
                     /gene="deoD"
                     /locus_tag="LP543_000162"
     CDS             71629..72336
                     /gene="deoD"
                     /locus_tag="LP543_000162"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBL2320361.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(72634..73320)
                     /gene="gpmA"
                     /locus_tag="LP543_000163"
     CDS             complement(72634..73320)
                     /gene="gpmA"
                     /locus_tag="LP543_000163"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBL2320362.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(73492..74073)
                     /locus_tag="LP543_000164"
     CDS             complement(73492..74073)
                     /locus_tag="LP543_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2320363.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(74206..74889)
                     /gene="rpiA"
                     /locus_tag="LP543_000165"
     CDS             complement(74206..74889)
                     /gene="rpiA"
                     /locus_tag="LP543_000165"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBL2320364.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            75208..75621
                     /gene="rpsL"
                     /locus_tag="LP543_000166"
     CDS             75208..75621
                     /gene="rpsL"
                     /locus_tag="LP543_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBL2320365.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            75705..76175
                     /gene="rpsG"
                     /locus_tag="LP543_000167"
     CDS             75705..76175
                     /gene="rpsG"
                     /locus_tag="LP543_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBL2320366.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            76269..78356
                     /gene="fusA"
                     /locus_tag="LP543_000168"
     CDS             76269..78356
                     /gene="fusA"
                     /locus_tag="LP543_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBL2320367.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            78528..79715
                     /gene="tuf"
                     /locus_tag="LP543_000169"
     CDS             78528..79715
                     /gene="tuf"
                     /locus_tag="LP543_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBL2320368.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            80122..80430
                     /gene="rpsJ"
                     /locus_tag="LP543_000170"
     CDS             80122..80430
                     /gene="rpsJ"
                     /locus_tag="LP543_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBL2320369.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            80461..81090
                     /gene="rplC"
                     /locus_tag="LP543_000171"
     CDS             80461..81090
                     /gene="rplC"
                     /locus_tag="LP543_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBL2320370.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            81118..81741
                     /gene="rplD"
                     /locus_tag="LP543_000172"
     CDS             81118..81741
                     /gene="rplD"
                     /locus_tag="LP543_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBL2320371.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            81741..82031
                     /gene="rplW"
                     /locus_tag="LP543_000173"
     CDS             81741..82031
                     /gene="rplW"
                     /locus_tag="LP543_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBL2320372.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            82070..82903
                     /gene="rplB"
                     /locus_tag="LP543_000174"
     CDS             82070..82903
                     /gene="rplB"
                     /locus_tag="LP543_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBL2320373.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            82946..83224
                     /gene="rpsS"
                     /locus_tag="LP543_000175"
     CDS             82946..83224
                     /gene="rpsS"
                     /locus_tag="LP543_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBL2320374.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            83247..83594
                     /gene="rplV"
                     /locus_tag="LP543_000176"
     CDS             83247..83594
                     /gene="rplV"
                     /locus_tag="LP543_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBL2320375.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            83608..84264
                     /gene="rpsC"
                     /locus_tag="LP543_000177"
     CDS             83608..84264
                     /gene="rpsC"
                     /locus_tag="LP543_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBL2320376.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            84267..84701
                     /gene="rplP"
                     /locus_tag="LP543_000178"
     CDS             84267..84701
                     /gene="rplP"
                     /locus_tag="LP543_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBL2320377.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            84691..84879
                     /gene="rpmC"
                     /locus_tag="LP543_000179"
     CDS             84691..84879
                     /gene="rpmC"
                     /locus_tag="LP543_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBL2320378.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            84904..85170
                     /gene="rpsQ"
                     /locus_tag="LP543_000180"
     CDS             84904..85170
                     /gene="rpsQ"
                     /locus_tag="LP543_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBL2320379.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            85228..85596
                     /gene="rplN"
                     /locus_tag="LP543_000181"
     CDS             85228..85596
                     /gene="rplN"
                     /locus_tag="LP543_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBL2320380.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            85633..85941
                     /gene="rplX"
                     /locus_tag="LP543_000182"
     CDS             85633..85941
                     /gene="rplX"
                     /locus_tag="LP543_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBL2320381.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            85969..86508
                     /gene="rplE"
                     /locus_tag="LP543_000183"
     CDS             85969..86508
                     /gene="rplE"
                     /locus_tag="LP543_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBL2320382.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            86527..86712
                     /locus_tag="LP543_000184"
     CDS             86527..86712
                     /locus_tag="LP543_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBL2320383.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            86748..87146
                     /gene="rpsH"
                     /locus_tag="LP543_000185"
     CDS             86748..87146
                     /gene="rpsH"
                     /locus_tag="LP543_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBL2320384.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            87178..87714
                     /gene="rplF"
                     /locus_tag="LP543_000186"
     CDS             87178..87714
                     /gene="rplF"
                     /locus_tag="LP543_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBL2320385.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            87875..88231
                     /gene="rplR"
                     /locus_tag="LP543_000187"
     CDS             87875..88231
                     /gene="rplR"
                     /locus_tag="LP543_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBL2320386.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            88252..88752
                     /gene="rpsE"
                     /locus_tag="LP543_000188"
     CDS             88252..88752
                     /gene="rpsE"
                     /locus_tag="LP543_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBL2320387.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            88767..88946
                     /gene="rpmD"
                     /locus_tag="LP543_000189"
     CDS             88767..88946
                     /gene="rpmD"
                     /locus_tag="LP543_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBL2320388.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            88990..89430
                     /gene="rplO"
                     /locus_tag="LP543_000190"
     CDS             88990..89430
                     /gene="rplO"
                     /locus_tag="LP543_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBL2320389.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            89430..90725
                     /gene="secY"
                     /locus_tag="LP543_000191"
     CDS             89430..90725
                     /gene="secY"
                     /locus_tag="LP543_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBL2320390.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNVAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            90787..91434
                     /locus_tag="LP543_000192"
     CDS             90787..91434
                     /locus_tag="LP543_000192"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBL2320391.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            91625..91843
                     /gene="infA"
                     /locus_tag="LP543_000193"
     CDS             91625..91843
                     /gene="infA"
                     /locus_tag="LP543_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBL2320392.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            91876..91992
                     /gene="rpmJ"
                     /locus_tag="LP543_000194"
     CDS             91876..91992
                     /gene="rpmJ"
                     /locus_tag="LP543_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBL2320393.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            92010..92375
                     /gene="rpsM"
                     /locus_tag="LP543_000195"
     CDS             92010..92375
                     /gene="rpsM"
                     /locus_tag="LP543_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBL2320394.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            92403..92792
                     /gene="rpsK"
                     /locus_tag="LP543_000196"
     CDS             92403..92792
                     /gene="rpsK"
                     /locus_tag="LP543_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBL2320395.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            92872..93810
                     /locus_tag="LP543_000197"
     CDS             92872..93810
                     /locus_tag="LP543_000197"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBL2320396.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            93838..94218
                     /gene="rplQ"
                     /locus_tag="LP543_000198"
     CDS             93838..94218
                     /gene="rplQ"
                     /locus_tag="LP543_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBL2320397.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            94490..95329
                     /locus_tag="LP543_000199"
     CDS             94490..95329
                     /locus_tag="LP543_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL2320398.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            95305..96174
                     /locus_tag="LP543_000200"
     CDS             95305..96174
                     /locus_tag="LP543_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL2320399.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            96164..96961
                     /locus_tag="LP543_000201"
     CDS             96164..96961
                     /locus_tag="LP543_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBL2320400.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            97164..97910
                     /gene="truA"
                     /locus_tag="LP543_000202"
     CDS             97164..97910
                     /gene="truA"
                     /locus_tag="LP543_000202"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBL2320401.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT        30779 a        18159 c        20433 g        29116 t
ORIGIN      
        1 ttcatcagga gtgtttcccg attcgttttt ggttgccgta gcaactttta tatcatatca
       61 gagagacaag taattgtcaa ctctttttta aaagtttttt cgatttcgtt tttcgaaatc
      121 ttccagcaac tcatttatca taacttatcg tttgttattt gtcaagaact tttttgaagt
      181 ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacgactcag ttatcttatc
      241 atgtcatttc caacatgtca acaacttatt ttttattatt taataagtta ttggtgttct
      301 tttgaacaaa taataatata tcaacatcac atgtgaaagt caataagaaa atgcttattt
      361 atcaacattt ctagcttaca tgatcattgg ttagttctgg tcctttttcg ttcgtcttta
      421 taactccgag agacgtaaaa tccttttgtt ttcagtttaa tgttcatgtt cttgtcttat
      481 tcacaccaac tagatgtctc accttctaaa attttcctta acaaagtgct gcttttatct
      541 atttctgctt ttttttgcta gaataatagt aacaatatga ctctggagga tttatttatg
      601 aaaattttga ttgctgacga tgataaagaa atcgttgaat tattaagtat ctatgttcac
      661 aatgaggggt atgaagcagt aaaggcttat gatgggaaag aagcattatc taaaatcaga
      721 acaatccctg atattgaatt acttatttta gatatcatga tgcctgagat ggatgggatg
      781 caagtagtga aagagctacg aaaagaatcc caaataccaa tcattatgct gacagcaaaa
      841 actactgata tggataagat caaaggactg gtcgctggtg cagatgatta tgtgaccaag
      901 ccatttaatc cgttagaagt aatggctaga gtaaagtctt tattacggcg tacaaaaatg
      961 cagattgccc cagatcagcc tgatgtatta gaagtaaatg cattaatcat caataaagac
     1021 tctcatgaag ttaaaacaat cgatgggaaa gaaatccagt taacagcttt ggaattcggt
     1081 atcctctact tgcttgctag tcatcctaat cgagtattta gcgcggatga aatttttgag
     1141 cgtgtttgga aacaagaaag tattgtctct gctaaaactg tcatggttca cgttagtcat
     1201 ttaagagata agattgaaga agcaactggt ggtgaaaaag tcattcaaac cgtttgggga
     1261 gtgggctata aaattgacgc aagatagtaa acgagcaact gaaaaacgcc gtcggatcaa
     1321 tttgacttca aaagaaatca gcgaattgtt agcggaagga atcatcacga ttatcctttt
     1381 gcttctatta aatgtttcta ttctggttgt tatcagttcg gtgatcaaca gttcaccttc
     1441 tttgacgaat gcgatttggg attcaaagaa tatttttgca gaacgattga acactgattt
     1501 gttctggaac ggccggaatt ttattattcc ctttttcttt ctattagata ttggtgtttt
     1561 gtattggcgc ttgattcgaa gatatcgcca aatgcaattg cggcatatca tcagcgaact
     1621 tcactacatc gcaaacggaa attatgatca tcgtatccct tttgaactga gtggtgactt
     1681 aagccgagtg gtaacaagta tcaatggatt ggtggatagt accgtagcag cgattgaaga
     1741 cgaacgtaaa atcgaaaaat caaaagatga attgatcacg aatgtcagtc atgatatccg
     1801 taccccactc acgtccatca ttggatacct aggactgata gaagatggcc agtatcactc
     1861 agaagaagat cttttaaagt atacgcatac tgcttatatc aaagcaaagc agatgaaatc
     1921 tttagtggac gacttatttg aatacactaa ggtacgccag cctgctgtcc cggtgaattt
     1981 ttctgccttt gacatgatcc agttgatcga acagttagca gctgattttg agttggaagc
     2041 ttctaaaaaa aatatccaaa tacttgttca atcaaaagta gactccctta tcatggatgg
     2101 cgataccgaa aaacttgtac gtgtcttcaa taatcttttg acgaatgcgt tgaaatacgg
     2161 aaaaggagca acgaagatcg taatcgaagt agaacgtatc ggttcagaag ttgttgcaac
     2221 agtcaaaaat aacggagcaa tgatcccaca acaggcaatc gataatctat tcgaccgttt
     2281 ttatcgtgta gaagaatctc gttcacaagc aactggagga actggacttg gtttagcaat
     2341 cgctcaaagc attgtggctt tgcacggcgg atacatttat gcaaaatcag ataagcaatg
     2401 gacttctttc atcatgcacc ttccaatcaa gaaaaatgaa aaattaccta ttgatacaca
     2461 ggaagttatt gacgaaagct gaaatattcg ctattcttat tacaattatt agaataaatg
     2521 aaaaacacag aacccagtac actttattct ttttcagaga gctgatggca ggtgtaaatc
     2581 agcaaaggat aatttggaag ttccagttcg tttacgtgcc ctttcgaaat aagaaaggtc
     2641 ggttcaacag ccgttatgtg ttcaacaagt gcagctgacg ctgaacttgg gtggtaccgc
     2701 gaatttcaga ctgattcgtc ccaagaataa tgggatggat cagtcttttt ttattttcaa
     2761 aaaggaggaa atcatatgtt agatgtaaaa atgatgcgac aaaatattga agaagtacaa
     2821 gaaaaattga tgactcgggg cgtaaaaaaa gaaacattag ctgaattcat ggaactagat
     2881 gaaaatcgtc gtaagctact agtcaaaagt gaagaattaa aaaaatatcg aaatgatgtt
     2941 tctgctgaga tcgcccaatt gaagcgtgca aaagaagatg cttctgctaa aatcgctgaa
     3001 atgaaagaag tcggtggcaa catcaaagca ttggatactg aaattgctga agtggatgaa
     3061 aaattgaaac aaattgcaac tactttgcca aaccttcccc atgattctgt gccagttgga
     3121 acagatgaag aggacaatgt agaaatccgt cgttggagcg aaccacgtac atttgctttt
     3181 gaaccaaaac cacactggga aattgcagaa aatcttgata ttttagattt tgaacgtggc
     3241 gcaaaagtag ccggtagccg ttttgtctat tacaaaggat taggtgctcg attagaacgt
     3301 gctgtctaca acttcatgct tgatcagcat atttacgaac acggctatac tgaaatgatc
     3361 actccttata tcgtcaacag taaagcgatg tttggtacgg gacaatttcc aaaattcaaa
     3421 gaagatgttt tccaattgga gaatactgat ctaacattaa tcccaacagc tgaagtacca
     3481 ttaactaact attataatga tgaaatttta gatggtgcag atttacctat ttatttcact
     3541 gcactaagcc ctgcttttcg ttctgaagcc ggaagtgctg gtcgggatac acgaggactg
     3601 attcgtcttc accaattcaa taaagtagaa atggtcaaat tttcggatgt agagcattct
     3661 tacgaagaat tggaaaaaat gacagctgat gcagaagata tcttgaaaaa attgcatttg
     3721 ccttatcgtg tgatggcctt atcaactgga gacatgggct tttctgcagc taaaacttat
     3781 gacttagaag tatggatacc tgcacaagat acttatcgtg aaatcagttc ttgttctaac
     3841 tgtgaagatt tccaagcacg ccgcgcaatg atccgttatc gcgataatga tggaaaaatc
     3901 cactatactc atactctgaa tggttcagga cttgcagttg gccgtacagt agctgccatt
     3961 ttggaaaact accaaaatga agatggttct gtcacagttc cagaagcgct cgttccttat
     4021 atggggaatc tgaaagttat taaataaaga ggctgtgaaa aagccgctta gtttcgagta
     4081 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaattgga cttaataccg
     4141 agaaactggc ttttgaacac catttatcga gaggttgtga aatcagcgaa ttaacctgag
     4201 tattagagaa aaaaatggaa aaatagtccc tcatatttta ccgtttgttg aactaatacc
     4261 gagggtcagc tgattgaaca ccgtttagtg gagaggtcgt aagcctaccc cccagctcct
     4321 agttacaagg acaaattcaa aaaacagctc ttcatatttt atgaatgttg tacttgtgat
     4381 tcggaggtgg caagggaaca ctgtttattc aaaaagaggc tgggacagaa gcgtttaact
     4441 ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcttctta
     4501 tttccgaagg agttgcttct acttccgccg tttatacgtg tttagagcgt gagacaaaag
     4561 tgttctttac ttttgtccca cgctctttaa tgtttttaag attcaactga atagtttggt
     4621 gcttcttttg tgatttgtac atcatgaggg tgtgattctt ttaatccact gccgctcatt
     4681 tggataaatt gcgcttcatc acgtaattgc tgtagattgg ctgccccaac ataacccatt
     4741 cctgaacgca aaccgccgat catttggaaa ataatatctg caacacttcc tttataagca
     4801 acccgacctt cgattccttc tggtactaat ttatttgctt cattgacact accttggaag
     4861 taacggtctt ttgaaccttt ttccatcgcg cctaatgagc ccatacctcg gtatgttttg
     4921 aatcgacgtc cttggaagat ttcgaattca cctggtgatt catctgtccc tgccaacata
     4981 cttcctagca tcactgcatg tccgccggca gctaatgctt tgacgatgtc accagaatat
     5041 ttgatccctc cgtctgcaat aatcgctttg ccatattgac gagcaactga tgctgcatca
     5101 taaatagctg tgagctgagg tacacctaca ccggctacca cacgtgtcgt acaaatcgat
     5161 cctggtccga taccaacttt tacgacatct acacctgcat catataaagc ttttgtcgct
     5221 tcagccgtcg caacgtttcc tgcaatcaat gttgcatctg caaaagttga acggatttct
     5281 tgaatttttc ggatcacacc tgcactatgt ccatgagctg tatcgatgat gattgcatct
     5341 gcccctgctt ctaataaagc attcgcacgc tcaaatgtat cgcttgttac accaacggct
     5401 gctgctacaa gcaatcgacc atgttcatct tttgctgcat ttggaaattc gatcactttc
     5461 tcgatgtctt tgatcgtgat caatccgctt aaacgacctt cgttatctac gattggtaat
     5521 ttttcgattt tatgtttttg caaaatcttt tctgcatctt ttagagaggt acctactggg
     5581 gcagtcacta agttgtcttt tgtcatcaca tcagcaatcg gcatgtgata atctgttaca
     5641 aaacgcatgt cgcggtttgt aattatccct accaattttc tgttttcaag tgtttcaacg
     5701 attggcacac cactgatacg atatttgctc atcaattctt ctgcgtctgc tacaagatgc
     5761 tgtggcgtta agaagaacgg atcaatgatt acgccacttt cagaacgttt gacttttctt
     5821 acttcatccg cttgctggct gatcgtcatg tttttatgga tcacacctag tccgccttgt
     5881 cttgccatag caatggccat cttgctgtcg gtaacagtat ccatacttgc actgatgatt
     5941 ggaatattca acttaatatt tttagctaat tgtacgctca tatccacttc gtttggtaac
     6001 acgtgacttt ctgctggtat caataagaca tcatcaaaag taaatccttt tttcgtgaac
     6061 tttgtttccc agttggacat ttttagtacc actcctctaa tttagtttat ctattaaaat
     6121 aattgatttt ttcatttaag tcaatgatct acttggaatc aagttagctt tctcatagaa
     6181 taccaatata aagcgtttac atctgtgtgt attaaaagag attgtaaaaa agctgtcctg
     6241 catcaagtaa taagaacagc caatcaaaaa cagcttctca tgtttttgaa ttggttgggt
     6301 ttattaccgc agatgcaaac ttttgaacac cgtttagtgg agaggctgtg aaaaagctgc
     6361 ttagtttcga gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
     6421 ggacttaata ccgagaaact ggcttttgaa caccgtttat cgtaaaaagg gactgtgaca
     6481 agacttttgt cacagtccct tactattatt taactatgga taaacaaaat gaggttcttt
     6541 actaccccta gatatctagc aaaatagctg atctttttgc tagtttatct tcttgggacg
     6601 gataaacttc caataatccc gtattttttc ttcaagtagt aacggattgc taaagcagtt
     6661 gccccgataa tgataactac tactggatct aatactggat tgataactgg tgtcaatagt
     6721 gttgtcgcag aaaacagtac gacccaaaga agtgtcgttc cacctaggat aagcaatgtc
     6781 ttacccattc ctggtttctt gcttcgatct gctccgggat attcatattg atagatgtat
     6841 ttgtacatca tataaaagac ccagccaccg attgcagaac ctagaacgag tgtagaaaag
     6901 ccataagctg gcgttgttcc acgtgaaaac aatccggtaa tcgcaatcat gatacttaat
     6961 agaccaagca acagcaacgt attatctaac cacatcaata aaggggtgga tgaatttttt
     7021 acttctgctg gcttattgat gattgcttcc gttctttcag agactgtccc aaacaattga
     7081 cgggctgttc ttccgccttt ttgttctgca actaaagtag gcagaatgtc atgaagggct
     7141 tttacttttt cttcttcaga caaattcgct gcttctaaag attttttcaa atcaaaaatg
     7201 tactgttgat ttcttttggt taattttgtt tctaaactat tgttttcttt tacgattgct
     7261 cgtaattgtt ctggttccat tatctaatag tcctccttag ccgttccttt tctcaacggt
     7321 cgtcacaaaa gacattctac catattctac agctagtttg atagcccttt tacacattaa
     7381 agcggaataa cattacgtcg ccgtcttgta cgacataatc ttttccttct aatcgaacac
     7441 gtccagcttc ttttgctgca tgcatgtttc cgtaatgatc caagtcttca aaagagacag
     7501 tttctgcacg gataaatccg cgttcgaaat ctgaatggat gattcctgct gcttgtggtg
     7561 ctttgattcc ttttttgaat gtccaagcac gtacttcttg ttccccagca gtaaagtaag
     7621 tagctaagcc taacaaatca taagcagcac ggatcaactg atccaaacct gattcttcga
     7681 ttcctaaagc ttctaaaaat tccgctttgt cttcttcatc taattctgcg atttcttctt
     7741 ctgcacgagc actgaccacg atcacttctg cattttcttc tgcagcaaag ttgcgcactt
     7801 cttgtacata atgattcgcg tctgcatctg ctacgtcatc ttctgagaca ttggctacat
     7861 aaagaattgg ttttgtcgtc aataaaaata gtgatttaac gattttttgt tcttcttcag
     7921 taaattcgat cgttcgggca gattttcctt cttccagtac aggtttgatt ttatccagta
     7981 cagctagttc tgccactgca tctttatctt tggttttagc aacttttgct acacgagtat
     8041 agcgtttgtt gatagattct aagtctgcta acactagttc caagttgatc gtatcaatat
     8101 cttctagtgg atcaacacgt ccttctacgt gagtaatatt atcatcatca aaacagcgaa
     8161 caacgtgaca aatagcgtct acttggcgga tatggcttaa gaattgattt cccaaacctt
     8221 ctcctttact tgcgcctttt acgattccag caatatctgt aaattcaaaa gtcgtcggaa
     8281 cagttttttt cggatgtaca agttctgtta agcgttgcaa acgccagtca ggtacttcta
     8341 ccatccctac gttggggtcg atcgtcgcaa agggatagtt tgctgcttct gctcctgctt
     8401 ttgtgatcgc attaaacaaa gttgattttc ctacgttggg caacccaaca attccagcag
     8461 tcaatgccat aaatttattc actctttctc tgcatttttt tcaattactt tttttaattt
     8521 cttttcaaaa tctcgacggg tcatcatgac gatatgtccg caattcatac atctgatttt
     8581 gatatctgcg cccattcgaa tgacttccca gcgattcgtc tggcaagcgt gaggtttttt
     8641 catttcaacg atatcgtgca agtcgtacat aaaaatagcc tcctttttgt ttattcttca
     8701 tcaaattgaa tattcaagat atcaagaatt cgagtcaaat ctttttgaga aagatattca
     8761 atctcgatct tacctttccc atttttttct tggatagcta cacttgtacc aaatttatcc
     8821 atcaaacgat cttcgctttc tcggatataa tatggttttt cctttgctag ccttgggatt
     8881 tttttctttt cttttccatc attttgattt aactcagaaa caagctgttc cagttggcgg
     8941 acagttaaat tttcagttac tgcacgattg gctaatttca agatcaatgt tttatttttt
     9001 aaacctaaca gcgtacgggc ttgtcccata gataaacgct gatcttgtac catttccttg
     9061 acaagatccg gtaaagaaag caagcgcaaa taattggcga tataaggtct gctctttcct
     9121 aaacgactag ctacttctgc ttgtgttagt ttcaggtttt tcatcagcat ctcatatgct
     9181 tctgcttctt ctaacggatt cagatcttct cgctgcaagt tttccagcac tgctacttgc
     9241 atcatcgctt cttcgtcaaa gtctctaacg atagcaggga tcgtttcttt ttcagctagt
     9301 ttggacgctc gaaaacgtcg ctcccctgca ataatctcat atccttttac tgcggaacgg
     9361 cgtacgatga tgggttgaaa gacacctgat tgttcaatag aattagctag ttcctgcaaa
     9421 gatacttctt caaatgtttt acggggctga tacggatttg gtcgcagctc attcaatggt
     9481 agctggacaa cggtatcatc ttttacatcc accgtctcta agttttctaa atcttgaaac
     9541 agtgcatcga ttcctcggcc taatccttta cttttcttca cgacttacca cttcctttgc
     9601 caatgcttgg tagacttcag ctcctcgaga acgtggatcg tagtcgatga tcggttttcc
     9661 atgactaggt gcttctgata aacgaatatt tcttggaatg atcgtttcgt aaactttttc
     9721 gcggaagtat ttacgtactt cttctacgac ttcatttccc agatttgttc gtgcatcata
     9781 cattgtcagt aagactccct cgatctctaa ttctggatta aagtgttttt ggactaaacg
     9841 aaccgtattc agtaattggc ttaatccttc caatgcgtaa tactcacact gaacaggtat
     9901 caaaatcgaa tcacttgctg taaatgaatt gattgtcaga tggccaagag aaggcgggca
     9961 atcgatcaaa atataatcat actgactgct tacttctgct aaagatcctt ttaatcttga
    10021 ttctcttgcc atcattgacg tcaattcgat ctctgctcct gctaattgga gagtcgcagg
    10081 cacaatactc aaattttcgt gttcagtgat caaagttgcc tcatcaattg gcaattcatt
    10141 gactagaaca tcatagatgt ctcttgtcac atcaggtttt ctgatcccga caccactggt
    10201 agcatttcct tgtgcatcca tatccactag caatactttt ttgccaaggc tagcaagaca
    10261 agcacctaga ttcaccgtcg tcgttgtttt gccgacgcca cctttttggt tcgcaacaga
    10321 tattatttgt gccatctctt ctcctcctat ttgattggct gcttattagg cagtccgggc
    10381 tttcttgggt atttctttgg ggttggtttc tttttttgaa taacgatgat atgccgttga
    10441 tcagagacat tcggcaaggt aaccgcctct tctttgagga atttaccacc taataaagca
    10501 atcgcttgtt ttgcttctgt caattcctct tcactttttg ctgctttcaa tgcataaaaa
    10561 tagccttctt gtttagccaa gggcaaacac aattccgcta acacattcaa acgagcaacc
    10621 gcacgagcag tgacaaggtc aaatgatgca cgaaagtgag gattttgccc aaatgtttct
    10681 gcccggtcat gatataagga agcatcgatt cctaataatc gggttaactc agataagaag
    10741 gttattcgtt tattcagaga atcaacgatc gttatctgca gttctggaaa ggcaattttt
    10801 aaaggtatgc taggaaaccc tgcccccgaa ccaacatcgc ataaagataa aggttgcgta
    10861 aagtctactt caaatgctaa tgtaagtgaa tcgtaaaaat gcttcagata tacgtctttt
    10921 tcatccgtaa tagctgtaag gttcatcttt tcgttccatt cgactaatgt ccgataataa
    10981 atagcgaatt gattcatttg atgatcggta agctctatcc ctttttctgc caaggattgg
    11041 cgaaattctt ctggtatcat aaatattcct ttcattgtga aacagcaatt gtttcacaga
    11101 ttctctccct actttaacgc aaaatctcta gacatgcaat ccttccatat ggattcttaa
    11161 aaatttgaaa agtaagaaaa gccgatagac tttttctttc tagtatgact cataaaatat
    11221 acacagaaga gagaactagc tttgagcaat ttctgcaaaa actagttctc tcttctattt
    11281 attattacgt attacgctgg ttcactgact ttcgcaatct tcccttgttc gatatagacc
    11341 attaggatac ttaggtctgc agggtttacg ccactgattc ggctagcttg ggcaattgtt
    11401 tctggttgga ttttctgcaa tttttgtttt gcttctgttg ctaatccgtt gattgctgcg
    11461 tagtcgatgt tttctggaat ctttttcgct tccatgcgtt ttaatttttc tactttttcc
    11521 atggcttttt tgatgtagcc ttcatatttg atttggactt ctacttgttc gatttctttt
    11581 cgtgtcaatt cttcattttc tggaataaac cgtagaagtt ctgcataagt aatctcagga
    11641 cgtcttaaaa agtcgcttgc tagtacacca tcttttaatg gtgccgcatt aatcgttgct
    11701 aaaaagtctt ggacttcttc tgttggtttg attcgtacat tcatcaaacg tttgatttct
    11761 tgttcaactg cttctttttt cttcaaataa ttcttgtatt gatcttcttt tactaagcct
    11821 acacggtgac ccatttctgt taatcgcaaa tctgcattat catgacgtaa aagcagacgg
    11881 tattcagcac gtgaagtcag caaacgataa ggttcgttcg ttccttttgt taccaaatca
    11941 tcgatcatga caccgatgta accgtcactt cgtttgatca ctaaaggttc ttttccctga
    12001 actttcaaag ctgcattgat accggcaatc agcccttgtc cagccgcttc ttcataaccg
    12061 cttgtaccat ttgtttgtcc agctgtataa agattttcaa cgactttagt ttctaatgtc
    12121 ggacggagct ggtgtggctc tactacgtca tattcgatag catatcctgt acgcatcatt
    12181 tctacttttt ctaatccagc aatagaatga agcatgttga cttgaacatc ctctggtaaa
    12241 gaagtagata aaccttgaac atagacttct tccgtattta agccttctgg ttccaaaaat
    12301 aattgatgac gtggtttgtc tgcaaaacgc acgatcttat cttcgatgga tggacaatag
    12361 cgagcgccta ccccttcaac gatccctgta aacattgggg cacgatgtaa gttgtctcga
    12421 ataatttggt gagtcgtctc acctgtatag gtcagccagc aagattcctg atctagttta
    12481 taggcactat ctggtgtgct gaagctaaaa tgattcggct ctttgtcacc aggctgctct
    12541 tctgtaacag aatagtcgat cgtacttgat tttactcgag gcggtgttcc tgttttgaac
    12601 cgttcgatag caaagccaag ttcttttaga tgattcgcta gtccaataga aggttgagag
    12661 ttattcggac ctgatgaata tttcaattca ccgataatga tttctccgcg aagtgctgtt
    12721 ccggctgtaa taacaactgc tttggcccgg tagctcgctc ctgtggacgt cactactcca
    12781 tgacaaacac catcttcgac gatcagttct tcgacgattc cttgtcttag cgtcaaattt
    12841 ggttcttttt cgatcgtatg tttcatttct gttgaataag catgtttgtc tgcttgtgct
    12901 cgcaatgctc ggacagctgg gccttttcct gtgttcaaca tacgcatttg gatataggtt
    12961 ttatcgatat tcttacccat ttctccgcct aacgcatcga tttctcgaac gacaacgcct
    13021 ttagctggtc cgcctacaga aggattacat ggcatgaatg ctaccatgtc caaatttatt
    13081 gtcagtaata atgtttttga tcccatacgt gcagcagcaa gtgcagcttc tgaaccggcg
    13141 tgtcctgcgc caacaacgat cacgtcatac gcatctgctt cataatgttc caatcgtttg
    13201 ttcctccttt tattttccta aacaaaattg actgaataat tgagtgatta gctcatcttg
    13261 tacactgtcg ccgacaactt ctcctagata gtcccagcaa cgcgtcatat ctatttgaac
    13321 gagatccaca ggcattcctg aatcaatacc tgcaatgact tcttcaagtg agagggaagc
    13381 tttttctaac aacgcgatat ggcgggtatt agaaacataa gtagcgtctc tttctcctgt
    13441 ttctccgctg aagaataaat cagaaatagc tgattccaaa cgatctagtc cgtcgttttt
    13501 agctacggaa attgaaaaaa ctggttcatt ttcgatcaat ttcttcaatt tttcttgttc
    13561 cagctgtgct ggcaaatctg ttttatttag aagaatgatc cgtttcaatc cacttgtcgc
    13621 ttccaataat tgttcatctt ctgctgtcag tggttcgctt tggtttagaa caagtaagat
    13681 caagtctgct tctgctaatg cttttcggct tctttcgacc ccgatccgtt ccaccacatc
    13741 ttctgtttcc cgaattccag ctgtatcgat tagcttgagc ggtacccccc gaacattgac
    13801 atactcttcg attacatctc gagtcgttcc tgcaatatcc gtaacgattg ctttttcttc
    13861 tcttaacaaa tggtttaaca aacttgattt gccgacattt ggccggccga tgattgccgt
    13921 acttaaccct tcccgcagaa ccttcccttg cttggatgta gcaagtagcg cttggattct
    13981 ttgttggatc atctgcgctt tttcaagcaa taacttcgta gtcaattctt cgacatcatc
    14041 atattctgga taatcaatat ttacttctac ttgtgctaat gtctctaaaa tctcttgtct
    14101 caatgagcgg atcaaagcag acagattgcc gtctaactga ttaagagcta gtcccattgc
    14161 tttgtctgtt tttgcccgaa tcaaatccat cacagcttct gcctgtgaaa ggtcaacacg
    14221 accattcaag aatgctcgtt ttgtgaattc gcctggctca gctaatcttg caccttctct
    14281 taacaatagc tgcaaaattt gattgactac aacgatccca ccgtgacaat tgatttctac
    14341 tacatcttcc cgcgtaaatg tctttggtgc tctcatcacg gagaccatga cttcatcgac
    14401 aagctgattg ctcttgggat caacgatatg tccataatgg atcgtatgac ttggtacttc
    14461 tgataatcgt ttgtttccag attgataaac tttatctgct agttgcacag cttgatcgcc
    14521 acttaaacga acgatactga ttgcaccttc tccaggtggg gtagaaattg ccgcaatcgt
    14581 atcaaattct aaagtaattt cagccatttt ttcacttcct ttttcatttt gcgcacaaaa
    14641 aaagtgccca ctccaccctt tatgcacaat aaacggtgaa cttcgcactt tgactgcttg
    14701 ttcttgatta aattctggtc atagattatc acacttgcta taaggaatca agtgtttaaa
    14761 acaattagat gaagttgagc ctgttctgct tccattacct tatatttaat tgtccttgga
    14821 tgtctctttg cgttctttgg tttattcgaa gagaagatac tgtataaaca aggctttatc
    14881 tttcttaaaa aaaacggtac actaaatggt agcgctatct aaactataca cttttgaaag
    14941 gagaaaatat gtgagcccgt tcagatctgc tgcattggaa gaagaaagtg accctcggat
    15001 caacgatgtc gttttatcta cagggaatat caaccagaaa tatatcgtcc gcgatatcgt
    15061 ttttgttgtg gaatgcttag aagctgattt gtttgaccca tcttttccca aagatgacct
    15121 tttcttaaca acgaagagga aattaaagac gaaggtactt gaatatggtg cgaatgcagt
    15181 aatcaactgt cattttgagt atcagcaaaa aaaacgcgac ggaaagtatt ttctggaact
    15241 ttttgcttat ggaacagtga tccaatttat ccaaacaacg attggttaaa tgaaacagac
    15301 aaaaaaagcg gatcaaaact gaagacagtt tcgatccgct tttttctatc aaagttagtg
    15361 actacttcat cgtatactct gataagaatg atttctagta aaatatactt ggtttgaaaa
    15421 actttgtcaa cgtgatttat tcaaaaagta atggaatcaa taatattttt tagcaggttc
    15481 tactactaaa taacgataag gttcctcacc ttccgaatgt gtttttacat gttcatcttt
    15541 gcttaatgca gcatggattt gtttacgttc aaatgcaggc attggttcta agaaaacagg
    15601 ctgccctgtc cgttttactt tttcagccgt gcgatcagct aaacgctgaa tgatctcttg
    15661 gcgcttttca cggtaattgc ctacattgac tactacagac agcttatttc ttgctaaacg
    15721 atggatgaag acttgtgcca gatattgcaa tgcattcagt gtttttccat gtttcccaat
    15781 cagtatgcct tgcttttgtg tttctaaatg gaaaacaacc atattatttg attctctttc
    15841 cattttgaca agagctggag catcaagctg ttttgaaatt tcagtaagat aaactgccaa
    15901 ttccgttaaa gctgcttcat cacctagctc acttacttct tctgtttttt cttcaagaat
    15961 cgtttcgttg actacctcga tctcagctgt tggttcttct tccaaaattt cttctacagt
    16021 ctgttctact gtttcagaaa ctttttcgct gatacttggc tggatcgaaa gccgtgcatt
    16081 ctttcgaccc attcctagga aacctttttt tccttcttcc agtatttcga tttcgacttg
    16141 gtctttggcc aaacccaaat cagccagacc tagttggaca gcttcgtcta ttgttgcagc
    16201 ttcataaatc ggcattagca ttacctcccc tattatttcc gttttttctt aggattcatt
    16261 gctttcttaa gagcacgttc tctttcgcgg attctccgtg cttcttcttc acgctctttt
    16321 cgaattttga aagggttgtt gatcagcagc gtttgtacaa cttggaaggc attggaaact
    16381 acccagtata atgaaagacc gctggctaaa ttgacaccca tcactaaaat cattacaggc
    16441 atcacgaaat tcatgatctt cattgatgca ttcgattcaa tttgactcat gctagataaa
    16501 tacgtacttg caaaagtaaa gattgctgct aaaatcggca aaataaatgt tggatccgcg
    16561 ttccctaagt tcaaccataa gaaatgccct tgtttcaact ctggtacacg tgaaatagat
    16621 tgccataacg ccatcaagat tggcatttgt actaataaag gaagacaacc agcatatgga
    16681 ttgacgccat tttctgcata taaacgctgc tgctcttctc ttaacttact ttgtgtttcc
    16741 ggatctcttg aactatattg ttgctgcagt gcttttaatt tcggttgtaa ttcttgcgtt
    16801 ttacgcatgc tctttgtttg gaaatgcatc aacggcaata ggatcacgcg gatgatcagt
    16861 gtaaataaaa tgatccctat ccctgcatta ccaaatgaca acgccttgat cgcctcagca
    16921 aagtaataaa caatataacg atcccaaatt cctgtactct gtgcgctgac ttccccagtt
    16981 ccgcaaccgg ataagacgat gaccaaacta acgataccgg ccatcaaaag taaacgtttg
    17041 tatttcttca cttactcttt tcctctctgt cgattatttt tgcaagtttt aagacgtgga
    17101 ctaaattgga gcgaacctca tcataagtca accccttcac acttggtcta gcaatcacaa
    17161 taaaatccaa ctccggatca atttcttctt ttatagaata aactgctgct cggatttttc
    17221 gtttgacttc attgcggttt accgcgttgc cgaccttttt tcctacagat aaaccaactc
    17281 gaaaatgagg ctgtcccggt ttttccaaac ggtaaacgac gaatttgcgg ttagcaaaag
    17341 aatctcccgt atggaacacg gcttgaaact ctcgttcttt ttttactcga tacgcttttc
    17401 tcattagttt tctttccttt tctatctctc acttacgctc ataatcatac cataattacg
    17461 attgtccttt catcatttcc ctaaaaaaga gtataaaaaa aaccactgaa accccagtgg
    17521 cctaagctga aagaactttt cttcctttgc gacgacggct agctaacaca cgacgtccat
    17581 ttttagtact catacgttta cggaatccgt gaactttttg acgtttacgt ttatttggtt
    17641 ggtatgttct tttcatatat tccacctcca aaatgttctt atctatttta taaacacaga
    17701 catactttga tagtataacg acaaagtagt gattttgtca atataaaata gaagatcgat
    17761 tgttaaaact tatccacaag attatttttt tctatgggaa tcctatgtgg aaaaagttat
    17821 gcacagggat tatccacaga taataaaagg ctggttttac acagaaaaca cacgtgtaaa
    17881 aaaagtaatc cacaatcagg cgatttcctg tgtattccgt ctcctaacta ttggtatatc
    17941 tgtatttttt atccacagac gcaagaggaa atctattggt ttttacgagt tttccacctg
    18001 tgcattagtg gtgtggaaaa ctttttttga caactgtgtt tttctctttt ttctttttgt
    18061 cgattgtgtg gataattgta attaaaaaat ttttttcttc ggtttctgtg gaaaactatt
    18121 gaaaaaaatg ctaaattagt actagttatt cactttttaa tgaaggagga ggccttatat
    18181 ggtatccctc gatgctttat ggaatgaatt aaaagcaaca taccaaaagg atttatcgcc
    18241 tgctagctat aatacatgga tcgaaacagc gaaccctcga acccttgatc aaaaccagct
    18301 cgttgttgag gttcctagca agattcataa agaatattgg gaaaaaaacc tggcgacaaa
    18361 aatcgttgaa gtcggttata tgttatcagg aaatgagatt atccctcgct ttattactgg
    18421 cgaagaagca gagcaagaag aagttataga agaaaaaaat ccaaaagttg tggcacctag
    18481 tccgctaaaa aaagccatgc tgaaccctaa atataccttt gatacgtttg tcattggtaa
    18541 aggaaaccag atggctcatg cagcagcgct cgttgtagca gaagatccag gttctattta
    18601 taacccgctc ttcttttatg gaggcgtggg acttggtaag actcacttga tgcatgcaat
    18661 tggtcatcaa atgctgcaaa gtcagcctaa tgccaaagta aaatatgtaa gcagtgaaac
    18721 ttttgctaat gatttcatca attcgatcca aaacaaaaca gcagaagagt tccgtcaaga
    18781 atatcgaaat gttgatttac tgctagtgga tgacattcaa ttttttgcag aaaaagaagc
    18841 tacgcaagaa gagtttttcc atactttcaa tgccttgtac aatgaaggga aacaaatcgt
    18901 actaaccagt gatcggttgc caaacgagat tccgaaactg caagaacgcc ttgtttctcg
    18961 ctttgcatgg gggctgtctg ttgatattac accgccggat cttgaaacaa gaacagcgat
    19021 tttacgtaaa aaagcggcgg ctgaacgtct agagatccca gatgacacac taagctacat
    19081 cgcaggacaa attgattcta acattcgtga attagaaggt gcgcttgttc gagtgcaagc
    19141 ttttgctgcg atgaatagtg aagatatttc gactagtcta gctgcagacg cactgaaaac
    19201 gttaaaatca ggaaaaggac atcctcaatt gtcaatcctg cagatccaag aagaagtggc
    19261 aaagtattat cacatccaat tgaaagatct caaaggaaag aaacgcgtca aatctattgt
    19321 tgttcctaga caaatcgcta tgtatttggc acgtgagctc acagataatt ctttaccgaa
    19381 aatcggagct gaattcggtg gcaaggatca tacaacggtt atccatgctc atgaaaaaat
    19441 ccagcagctg atggattcta gtgtcagtat gcaaaatgaa gtatcagaaa tcaaaaactt
    19501 tttactaaat taatttgtcg attctatcaa ctgaaaatag catgtggata aaagaaaaaa
    19561 acgttcaaaa gttatacaca ctttttccac agctgttttt tcgtttctct cttacgtttt
    19621 ttgagttttc cacagaatca acatgcccta ttacttttat tattcttttt aactaataat
    19681 aaataaacaa acattcaaag gagatacatc atgaaagtta ctttaaaccg agctagcttt
    19741 atgcaggaat tgcaaactgt tcaacgagct atttcaagca aaaccacgat ccctattttg
    19801 acaggtgtaa aaatcacact gacacaagaa ggtttgactt tgacggggag caacgctgat
    19861 atatcaattg aaactttttt gtctgttgaa aacgaaaaag caaatatgca aatcgaatct
    19921 actggttcca ttgttttaca agcacgtttc tttagcgaaa tcattcggag acttcctgaa
    19981 gaaacattta ctttagaagt tttagaaaat aaacaagtag cgatcacttc tggaaaagcg
    20041 aattttatcg taaatggatt agatgcagat aactatcctc atcttcctgt tgtcgaaagc
    20101 cataaccaga tgaaattacc tgtacacgta ttgactaaac taatcaacga aacagttttt
    20161 gctgtctcac aacatgagag tcgtccaatc ttgacaggtg tgcatttcat tttatctgat
    20221 aattctttat tagctgtagc tactgattct caccgtctaa gtcaacgcgt gattccagta
    20281 gaacaagcgg ctgatcattt tgatattgtt attcctggaa aaagtttgat cgaattatct
    20341 cgctcattaa caaatgaaga agaaatcgtc gaaatcagca ttatggaaaa ccaagtgttg
    20401 ttcaaaacag aaacgatgta tttctattcc cgtttgctag aaggaaacta tccagatacc
    20461 aatcgtttga ttccttcaag ttttaataca gaggttgaat tttctgttcc tagcttttta
    20521 gcggcgatcg aacgggcttc tttactttct catgaaggcc gtaacaacat cgttcgttta
    20581 tctattcgtc cagatgcggt tgttttatac ggaaattcgc cagaaatcgg gaaagtagaa
    20641 gaaagcttga gttatacagc aagcagtggc gatccattag atatttcttt caatccggat
    20701 tatatgaaag cagcgttgcg tgcatttgga gatatgagca tcaaagtgaa atttatctct
    20761 gctattcgtc catttacatt ggaaccaacg gaagatggcg ttcaatttat tcagctgatt
    20821 actcctgtac gtacaaatta acaatgattt tcaatgttct tgaaaattta attgaaaaga
    20881 aatagaaaaa ataaaatttt aaacagaagg ccctaaaatc tcttttaagg ctttctgttt
    20941 atttttgttt aaatcacctt ttttagatta aatcgtctta tatggaaaaa tatatggata
    21001 aatttgtttt attgcgaaaa aaaaggtata atagagtata cttttaataa tgaaaatgag
    21061 ggatgggcat tgaaacaaca agtcattcta aatacggaat tcatgacgtt gggccaaatg
    21121 ttgaaagaag tcacagttat cggtagtggc ggacaagcaa aatggtattt ggcagaaaac
    21181 actgtgttag tcgatggcga accagaaaat cgtcgtggac gcaaacttta tcccggcatg
    21241 atggttgaag ttccagaagt aggaactttt tttatggtaa aaaaaggaaa cgacgataat
    21301 gaggctgaat aagctgtatt taaaaaatta tcgaaattac gaagagctga acatcgaatt
    21361 ttccaagaac ctcgtcatct ttttaggaga aaatgcgcaa ggaaaaacga atcttttgga
    21421 aagtatctac gtattagcta tgacgcgaag tcatcggaca agtagtgagc aggaattgat
    21481 tggctggact gacgatcagg cgatgattca aggagagatc acaaaaggtt catccactct
    21541 tccattagaa atcttattat ctaaaaaagg ccgaaaaacc aagatcaacc atatcgaaca
    21601 gaaaaagtta agtacttacg tgggacaaat gaatgtcatt ttatttgcac cagaagatct
    21661 ttctttagta aaaggcagcc cgcagatcag gcggaagttt ttagatatgg aattaggcca
    21721 gatcaatcca atctatcttt atgatcttgt ccaataccaa gcaattttga aacaaagaaa
    21781 ccagtacttg aagcaattga atgaaaaaaa acaaactgat ttgctttatt tggatatttt
    21841 gtccgaacaa ctagcagcgt ttggcagcaa agtattgaaa gcaagagcac agttcgtcaa
    21901 gcgattggag ttttgggcta attctttgca tcagcaaatc acccatcaaa aagagcagct
    21961 ggaaatcgag tatctcaccg cagtcgattc tttagaaaca catacgcaag agcaaatcca
    22021 agaacaattt ctcgcacttt tgaaccaaaa taaaaagaaa gacctttttc ggggaacaac
    22081 gacagttggt cctcatcgtg acgatctttc cttttttatt aatcagaaaa atgtgcagac
    22141 ttatggatcg caaggacagc aaagaacaac tgcactaagt gtcaaattag cagaaatcga
    22201 tttgattaaa gaagagacag gggaatatcc aatccttttg ttagatgatg tgatgagcga
    22261 gctggatgat aatcgtcaac tccatctgct agaaacaatc gaaggaaaag ttcaaacgtt
    22321 cttaacaaca acgacacttg accatgtaaa aaataaaatg actgttgaac ctgagatttt
    22381 ttatgttcaa caagggaaac taaaaggaga agaaactgaa tgacagaaga aagaagttta
    22441 gtagaacgcg ctaaagagta tgatgccagt cagattcaag tacttgaagg acttgaagcc
    22501 gttcgtaaac gacctggcat gtatatcggt tcgactagtt ctgagggtct gcatcacctt
    22561 gtttgggaaa ttgtcgataa ttcgatcgat gaagtattag caggttttgc aacgaaaatc
    22621 catgtgatta ttgaaaaaga caacagtatc accgttattg acgacggtcg ggggatacct
    22681 gtagatatcc aggcaaaaac tggacgtcct gcagttgaaa cggtctttac tgttttacat
    22741 gctggaggaa aattcggcgg tggtgggtac aaagtttctg gtggacttca cggagtagga
    22801 tcttctgttg taaacgcgct ttctacacag cttgatgtca aagtatacaa agaaggaaat
    22861 gtgtactatc aagaatatcg tcgtggagct gtagtggacg acttaaagat tatcgaacaa
    22921 acggatcgcc atggaacaac ggttcacttt acgccagacc ctgaaatttt tacagagaca
    22981 actgagtttg atttttcaaa attggctacc cggatccgtg aacttgcttt cttgaaccgc
    23041 gggatgcgta tatcgattga agacaaacga gaagaagaac cagtcatcaa tgaataccat
    23101 tatgacggcg ggatcaaaag ttatgtcgaa tacttgaatg caaacaaaac cgtgattttt
    23161 ccagaacctg tatatttgga aggtgaacag caagatatcg cagttgaagt ctctatgcaa
    23221 tatacagacg gctaccattc caatatcatg agttttgcca acaatatcca tacgtatgaa
    23281 ggtggaacac atgaatcagg attcaagact gctttaactc gtgtgataaa tgattatgcc
    23341 cgaaaacaaa agctgatgaa agaaaatgac gacaacttga ccggtgaaga cgtgcgagaa
    23401 ggattgacag ctgttatttc tatcaagcat cctgaccctc aattcgaagg acaaacaaaa
    23461 acaaaattgg gcaattccga agtacggaca gtcactgatc ggttatttag cgaacatttc
    23521 atgaaattct tgctggaaaa cccaagtgtg ggccggcaaa ttgttgaaaa aggattacta
    23581 gcttccagag ctcgtctagc agcgaaacgt gcgcgtgaag taacgagacg taaaggtgct
    23641 ttagagatta gtaatctgcc aggaaaatta gcggactgct ctagcaacga tcctgaaaaa
    23701 tgcgaattgt tcatcgtcga aggagattcg gccggcggat cagctaagca aggtcgtaat
    23761 cgagagtttc aagccatctt gcctattcgt gggaaaatct tgaatgttga aaaagcaagc
    23821 atggacaaga tcttggcaaa tgaagagatc cggtctttat ttactgctat gggtacaggg
    23881 tttggagaag attttgatgt atcgaaagct cgctaccaca aattagttat catgactgat
    23941 gccgatgtcg acggcgcgca tatccgtaca ttgctgctga ctctttttta tcgttacatg
    24001 cgtcctattg ttgaagctgg atacgtctat attgctcagc cacctttata cggggtaaaa
    24061 caagggaaga atattacgta tgtacaacca ggaaaaaatg cagaagaaga gttgaaacaa
    24121 gtcgttgctt cacttcctgc ctcaccaaaa ccaagcgttc agcgttataa aggacttgga
    24181 gaaatggatg atcatcaact atgggaaaca actatggacc caagcaatcg gatgatggcc
    24241 cgcgtcagtg tggacgatgc tattgcagca gatcagatat ttgaaatgtt gatgggggat
    24301 cgagtagaac cccgtcgtgc attcatcgag gaaaatgcac actatgtgaa gaacttagat
    24361 atttaatcat gaaggaggaa aaagaatgag tgaagaaatc agagaaaaca tccatgatgt
    24421 caatctaacc agcgaaatga aagactcctt catcgattat gcgatgagtg ttattgtcgc
    24481 acgtgcactt cctgatgtca gagatggtct gaaacccgtt catcgccgta ttttatacgg
    24541 catgaacgaa ttaggtgtaa caccggataa agcccataaa aaatctgcca gaatcgtcgg
    24601 agatgtcatg ggtaaatacc atcctcatgg agaccgtgcg atctatgagt caatggtacg
    24661 gatggcacag ccattcagct accgttatat gctagtggat ggtcatggaa actttggttc
    24721 tgttgacggt gacggtgccg ccgctatgcg ttataccgaa gcacggatga gcaaaatcgc
    24781 tacagaaatg ctgcgtgata tcaataagaa cactgttgat ttccaaagca actatgatga
    24841 tacagaaaaa gaaccagttg tactacctgc tcgcttcccg aatcttttag tgaacggaac
    24901 aacggggatc gccgtaggta tggccacgaa tatcccacct cataacttgt ctgaggtagt
    24961 agcggctatc gatttgctga tggataatcc tgatgtgacg accaatgaat tgatggaagt
    25021 attacctgga ccagattttc caacaggtgg tttggttatg gggaaatcag gaatccgaag
    25081 agcgtatgaa acaggtaagg gttctattac cgtacgtgca aaagttgaga ttactgagat
    25141 gccaaatggt aaagaacgga tcatcgtgac agaattgcct tatatggtca ataaagcaaa
    25201 attgatcgaa cggatttctg aactgcacag agacaagcgt atcgaaggga tcacagattt
    25261 gcgggacgaa tcttcccgtg aaggtatgcg gatcgtcatt gacatccgtc gtgacgccag
    25321 cgcatccgtt atcttgaata acttatataa attgacatcc ttgcaaaatt catttggatt
    25381 caatatgttg gcgatcgaaa aaggtgtgcc gaaagttttg agcttgaaac aaatccttga
    25441 aaactatatc gaacatcaac gcgaagtcat cacacgacgg acggtctttg aaaaagaaaa
    25501 agcagaagca agagcacaca tcttagaagg tttgcgtatt gctttggatc atatcgatga
    25561 aatcattgcg attatccgcg ggtctaaatc agatgacgag gcaaaagcaa caatgatcga
    25621 acggttcgat ttatccgatc gccagtctca agctatttta gatatgcgtt tacgccgatt
    25681 gaccggtttg gaacgggaaa aaatcgaaaa tgaataccaa gaactcttaa aactgattga
    25741 agatttaact gacattctgg caagaccaga acgtgttaca gaaatcatca agaatgaatt
    25801 agcagacttg aatcaacgat ttggtgataa acgacgcaca gaattactag tcggtgaggt
    25861 cttaagtctg gaagatgaag atttaatcga agaagaagaa atcgttatta cattgacaaa
    25921 caatggttac atcaaacgtt tggcaaataa tgaattcaga gctcaacgaa ggggtggacg
    25981 tggtgtccaa ggaatgggta tccacaatga cgacttcgtc aaaaatctgg tttcttgttc
    26041 tacccatgat acattgttat tctttacgaa taacggaaaa gtgtatcggg caaaaggata
    26101 cgaaattcca gaatacgggc gtacagccaa aggaatccca atcatcaatt tactaggtat
    26161 cgattcgagt gaaagcatcc aggcaatcat tgcggtgacg ggagaagcag aagaaggcca
    26221 ctatctcttc tttacgacgc gtcaaggtac agtaaaacgt acttctgtca ctgcttttgc
    26281 taacatccga agtaatggtc tgatcgcaat tggtttgaaa gaagacgatg agctcgtcaa
    26341 tgtgctactg acagatggtc aatctaatat cattattgga acacataacg gctactctgt
    26401 cactttcaaa gaagaagcag tccgcgatat gggacgtaca gcttccgggg tcagagggat
    26461 acgcctgcgt gaggaagatt atgtcgttgg ggcagcctta atgaaggaaa atcaagaagt
    26521 attagtgatc acagaaaaag gttacggaaa acggacaaaa gtatcggaat atccagtgaa
    26581 aggccgcgga ggtaaaggga tcaaaacggc gaacatcact gaaaaaaatg gtccactagc
    26641 tggattgacc actgtttctg gagatgaaga catcatgctg attacagata aaggtgtcat
    26701 tatccgtttc aacgtatcga ctgtttctca aactggacgt tcaacattag gcgtacgttt
    26761 gatgaagatg gaagctgata cgaaagttgt cacgatggca gcagtcgaac cagaagctgc
    26821 agaacaaaca gttgaagaac aaggatcaga agaataacca tcaagactaa gtgtgtaagc
    26881 tagcaagaat aaccgattaa aaaatctggt tattcctgct ggcttttttg attagttgaa
    26941 tttctcttaa aatattcgaa actgttcatt ctgtattgct tttgaccaaa atagagtgta
    27001 taattagagc ttgtgagtag tctaattaat tagataactc tccttgctct taggaaccta
    27061 agagccgaaa gtccacaggg aggtgaatat caatggaaaa cacgaaatat gaaatcatgt
    27121 acattattcg tccaaacatt gatgaagaag caaaaactgc tttaatcgaa cgtttcgatg
    27181 caatcttgaa agataacgga gcagaagtta tcgaatcaaa agattgggaa aaacgccgtt
    27241 tcgcttatga aatgaacggt taccgtgaag gtatctatca catcgttaaa gtatcttctc
    27301 catcatcagc aaatgctgtc aatgaatttg accgtcttgc taaaatcaac gacgatatca
    27361 ttcgtcacat gattgttaaa gaagaagctt aattgttgtt tcatgtgaaa caatagaaaa
    27421 cgatgagagg agatgactat tttgattaat aatgttgtat tagttggacg tctgacgaaa
    27481 gatccagatt tgcgttacac agcaagtggt acggcagtcg caacatttac tttggctgtg
    27541 aatcgtaatt ttacaaacca aagcggcaat cgcgaggctg attttatcaa ctgtgtgatt
    27601 tggcgcaaat cagcagaaac tttagcaaac tatgctcgta aaggaacact tttaggtgtt
    27661 actggacgta tccaaactcg ttcttatgat aatcagcaag gacaacgtgt atacgtgaca
    27721 gaagtagtag ctgacaactt ccaattgctg gaaagtcgtt ctgcatctga aaatcgccaa
    27781 caaagcggcg gattccaaag ttctggtcaa tcagctggcg gatttggcgg aaacaataac
    27841 tctaatcaaa catctcaatc atccaacggt atgcctgatt tcgaccgtga tacttctgac
    27901 ccattcggct caagttcaac gatcgacatt tcagacgatg atttaccatt ctaaacgaat
    27961 ttgataggag ggaaatagaa tggcacaaca aagaagaggc ggacgtaaac gtcgtaaagt
    28021 agactacatt gctgctaacc atattgaata tatcgattat aaagatatcg aattactaaa
    28081 aagatttatt tcagaacgcg gtaaaatctt gccacgtcgt gtaactggta caggcgctaa
    28141 aaaccaacgt aaaattactg ttgcaatcaa acgcgctcgt atcatgggct tgctaccttt
    28201 cgttagcgaa gactaataaa tcctaggaat tgaatgactg atccttttga tcagctattc
    28261 aattcctttt tttctgtaaa aataacctct attcgtttag tcgctcgaat gacgaaagtt
    28321 atggtaaaat caaattagtc atatatggga ggaaaattat ggaaaataaa aacaatcatt
    28381 tatccccgaa acctctcttc cttatcctgt ttatattaat cgaagtattg gtagtcttaa
    28441 ttttaccatt tcgttttgtt agcgcttcgg tcattctatt gatgaattta tggggtgttc
    28501 gctattggtt gaaaatgaaa cgttggctta ttttaaataa acgtgaaaaa atccagcagg
    28561 cttcgactgt agctgaaacc aatctagctt atgtctcaga agtcatgcct gtaggagtga
    28621 ttgcttatca gccatcaagc caaaaaatcg aatggctcaa tccattagca ttgaaaatca
    28681 aagatcaaag tggattaaca gaggaagaat tgctagatac tttttttact gcaaaagaaa
    28741 aaaagaaaaa ccaagtctat ctagcagata aaacatttca gtttgatatg gatacagtaa
    28801 agggtgttgt ttattttaat gatattaccc aaaaccagtt gttaaaactc catcagacga
    28861 acagtcagcc ggtgattggt attttatcta ttgataacta tgcagatgcc attgataaat
    28921 tagatgacaa agagatttct tatttgaact ctttcgtcac tacactagtt tctgactgga
    28981 tgaatgaata ccatgtcttt ttcaaaagaa tcaacgcaga acggtttttc ttcgtagcac
    29041 agatgatcga tctgaaaaag atgatggata aaaaatttga tttgctggat cgctttcgag
    29101 cagaagctga aaaacagagc tcgcctatca cccttagtat ggggattgct tatggctcaa
    29161 gaaccctcga tgaaatcggt ggagaagctc agaataattt agatgttgct ttagtccgtg
    29221 gaggcgacca agttgtcgtg aaagaggtaa aagaaggagc aaaacctgtt tattatgggg
    29281 ggaaatctgc taagaatgtc aaacgcacgc gtgttcgttc tcgagcgatg agtaccgctt
    29341 tgcgtcggat ttttgatgaa acaggcgatg tgtttatcat ggggcacaag tttccggata
    29401 tggatgcttt aggctctgct tttggtgtca gttgtctggc acgcttcaac cagaaaaaag
    29461 cttacatcgt tatcaatgag aacgagatca ttccagatgt agaacggtgt cttgaagaaa
    29521 tccataagca tcctgaatta gaaatgcaac taatctctcc agaacgtgcc atagagctga
    29581 aaaaagaaga ggacctcctc gtgatggtgg attaccataa accttctcta tcgatctctc
    29641 agggcctata tgagcaattt gataagattg tgatcattga tcaccatcgt cgtggggaag
    29701 agtttccaag taaacctctg ctgacttata ttgaatcttc agcttcttca gcttcagagc
    29761 tagtttctga gctgatccaa taccaaagca gcaaaacaaa tcgcttagat cgttttgagg
    29821 ccacattatt gcttgcaggg atggttgtag atacaaatag tttcaatgtc cgtacaactg
    29881 cacgaacttt tgacgttgcc agctatttaa ggtcttgtgg agcagacgca tccttagtac
    29941 aatatttatt gagctcggat ttagcgacat acttggagat cagcaatttg attgctggaa
    30001 gcgactatgt gacttctgat attgttgtgg cggcagggac agacgaaaaa gagtataata
    30061 gtgttatagc agcaaaaaca gcagatacat tgctttcgat gatcaatatc aatgcagctt
    30121 tcgtcattac aaagcggaca gacggcttga ttgggatcag tgccagaagt accggcacga
    30181 tcaacgtcca aatcatcatg gaatccttag gtggcggagg acacttcaca aacgcagcta
    30241 ctcaactgga aaatacgaca gttgaggaaa caaaacagaa gctatttgct gcaatcaaag
    30301 aaaatatgaa tcaaatatat gataaggagt aatccaaatg aaagtaattt ttttacaaga
    30361 tgtaaaagga aaaggtaaaa aaggggacat caaagaagtt ccagcaggtt atgcacaaaa
    30421 ttatctaatc aaaaataact tggcaaaaga agcaacgaaa agcagtatca gtcagttagc
    30481 aggacaaaag aaagcacaag aaaaacacga agctgaaatc ttggaagaag ctaaaaagtt
    30541 aaaagaattc ttggaaaaag aagatactgt agtcgaattg aaggcgaaag caggggaaga
    30601 cagccgtttg tttggttcga tcccttcaaa acaaattgct gaagggttaa acaaacaata
    30661 caaaatcaaa ttagataaac gaaaaatcga attagaaaat ccgatccgtt cactaggcta
    30721 cacaaacgta cctgttaagc ttcatcatga ggtgacggct acgatcaaag ttcatgtcgt
    30781 acaagaataa caaatcttga tagcgatgtt cagctggtaa gtataaccag aaggaaaaaa
    30841 gacaaaccga aatttatctg agaaaacagc tgcagcaatg aaagcaaaag ctgcaaatcg
    30901 atttcagatt gatttcggtt tcttttttgg gaacagcaaa atagcataaa gaaaacgagt
    30961 ataataaaag agaactattt ttttattaca ctttcggtac gaatgatgac ttttctctca
    31021 ttttcttgta aaatgaagca attgaagaca aggagcgata cgtatgaatg aagtgtggca
    31081 ggatcgaatc ccaccgcaaa atattgaagc tgaacaagcg gttttaggct ctgtttttct
    31141 tgacgcagaa acgatcattg atgccatgga gtacatagag ccaaaagact tttatcgtcg
    31201 tggccatcaa attatttttg agacaatgat cgagttaaat gatcgtaatg aagcgattga
    31261 cgtggttact gtcaaagacc ggctggaaca agccaatttg ttagaagatg ccggtggttt
    31321 aagttatctc tcagacttgg cattagcagt tccgacagca gcaaatatcg tctactatgc
    31381 gaaaatcgtt gaagaaaaat cattgctgcg tacattgatc cagacagcaa caggaatcgt
    31441 tacgaaagga ttcgagcaag gagaagaagt tcaaacaatt ttagatgatg ccgaacgcag
    31501 tatcttggag gtttcggaaa aaagaaatcg aagcggcttt ttatctattg ctgaagtact
    31561 gaactccacg attgaaaata tcgatcgact agctcaaaac aatgaagaaa tcacaggtct
    31621 accaactggc tatcaaggtc tggataaaat gacagcaggt ttccagcccg aagaactaat
    31681 cattcttgct gcacgtccag cggtaggtaa aacagcgttt gcattgaata ttgctcaaaa
    31741 tgtaggaaca aaaacagatc gttctgttgc gatatttagt ttggaaatgg gggcagaatc
    31801 tcttgtaaat cggatgcttt gtgcagaagg ttcgattgag gccagccatt tacgtacagg
    31861 tcagctttcg gaagaagaat ggtcgaatct gatcgttgcg atggggagtc tttctcgagc
    31921 aaatattttt atcgatgata caccagggat caaaatctct gaaatccgtg cgaaatgtcg
    31981 gaaactagcg caagaaaaag gcaatctagg cttgatattg atcgattatt tgcagttgat
    32041 tgaaggtaac ggtaaagaga accgtcaaca agaagtatca gatatctctc gccaactgaa
    32101 aaaactggcg aaagagctga aagtacctgt cattgctctt tcacagttat ctcgtggagt
    32161 agaacagcga caagacaaac gaccagtgct aagtgatatt cgtgaatccg gatcaattga
    32221 acaagatgcg gacatcgtag ccttcttgta tcgagatgat tattatgaaa gagaaggcgg
    32281 agatgacgat gatggaaacg agccaaaaaa tgacaacatc atcgaagtca tcatcgagaa
    32341 gaaccgaagc ggtgcacgag gaacagttga actactgttt atcaaagaat acaataagtt
    32401 ttcgtcgctt tcgccaagag aagaatttta aagaagaggt tgtgagcctt gagcaatccc
    32461 gagagctctc gtaagaagat gcaaggagta accgcaggaa cgactagagt acttaattta
    32521 aacagatctc caaagggatt gtgacaaaag tcttgtcaca gtcccttttt ccgaataaac
    32581 ggtgttcaat cagctgaccc ttggtattag ctcagcaaac gtcaaaatat gaggaactat
    32641 tttgacgttt gtttctctaa tactcaggtc aaaacgctga tcttacgacc tcttttcctt
    32701 aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatcatcaa acatgagaag
    32761 cagttttttg aaattgctcc ttgtgagtca gagttaaacg gcaggctcac aacctctttc
    32821 ttaaacggtg ttcaaaagct tgcatctgcg gtaatacgcc caatcaaacg aaaaatatga
    32881 gaagctattt ttgtttggct gttcttatta cttgatgcag gacagctttt tcacaacctc
    32941 tgattcacgg tgttccaaaa gtagcttctt cgaaaaataa gtcgaaattt cacaaaaatt
    33001 tgagaagcaa ttttcgtaca ttccttctta tttcttgaag ctgaccactt ctgttacaac
    33061 ctctttttta ataaccattc taaaaagata aagttcgttt tttaagcgtt tttataaaat
    33121 aaaaataaaa atcattcgat tttatttgaa atatgtatct attattgata caatggttca
    33181 ggattaaata atatagaacg aggtgttcac atgtcatctg tagtagtagt aggtacgcaa
    33241 tggggcgatg aagggaaagg aaaaatcaca gactttctca gtgaaaatgc tgaagtgatt
    33301 gcacggtacc aaggtggcga caatgcaggg cacacaatta agtttaacgg tgtgacttat
    33361 aaactgcatt tgattccttc tgggattttt tataaagaca agatcagtgt gatcggaaac
    33421 ggcgttgtag tcaatcctaa atcattggtt aaagaattag cttatttaca tgaaaaaggt
    33481 gtagaaacaa gtaatttgcg tatttctgac cgcgcgcacg taatcttgcc ttatcatatc
    33541 aaattggacc aattacaaga agatgctaag ggcgaaaata agattggtac aacgattaaa
    33601 ggaatcggac ctgcctatat ggataaagca gctcgtgtag gtatccgtat tgcagactta
    33661 ttagataaag aaatttttga agaacgtctt cgtttgaatc tagatgacaa aaaccgtcaa
    33721 tttgtaaaaa tgtttgattc agaaccactt gattttgacg atatttttga agaatattat
    33781 gaatacggac aacaaatcaa acaatacgtc acagatactt ccgttatctt gaatgatgct
    33841 ttggatgcag gcaaacgcgt gttgtttgaa ggcgcacaag gtgtaatgct ggatatcgac
    33901 cagggaactt atccttttgt tacttcttct aacccagtag ccggtggggt aacaatcgga
    33961 agcggtgtag ggccttcaaa aatcaataaa gttgtcggcg tctgcaaagc ctatacgtca
    34021 cgtgtcggtg atggaccatt tccaactgaa ttatttgatg aaacaggaga acaaatccgc
    34081 gaagtaggac gagaatacgg tacgacaact ggtcgtccgc gtcgtgtagg ctggtttgat
    34141 tctgtagtta tgcgccattc taaacgagtt tcaggtatta ccaacctatc tttaaactcg
    34201 atcgatgtat taagtggatt ggaaacagta aaaatttgta cggcttatga attagatggc
    34261 gaactaatct accactatcc agcaagcttg aaagagttga accgctgcaa accaatctat
    34321 gaagaattgc caggctggtc agaagacatt acaggatgca aaacacttgc agaactgcca
    34381 gaaaacgcac gtaactatgt acgtcgtatc tcagagcttg ttggtgtacg tatctcaaca
    34441 ttttctgtcg gacctgatag aacacaaaca aacatcttag aaagcgtctg ggcgcaaatc
    34501 taaggataaa aacgacgact gaatcaggac gttcttacca gagcgagtcg aagtgtttat
    34561 ctatacgata ataataactt gaagaggtag tgaaagagac gtttagcctt attgccgata
    34621 ggctgtctct tgaacactgt ttattcagaa taagggactg tgacaaaaac cgtgtcacag
    34681 tccctttttc cgaacaaacg gtgttcaaaa gcttgcatct acggcactaa gcccaatcag
    34741 atgaaaaata tggagtgcta tttttatctg attgttctta ctgcttgatg cagagcagct
    34801 ttttcacaac ctctccacta aacggtgttc tcctgccaac tcctcggtca caagtcacaa
    34861 tattcaacaa acatgagaag ctgttttttg aaattgttcc ttgtgactcg gagctaaacg
    34921 gcaggctcac aacctcttgg taaacggtgt tcaatcagct gaccctcggt attagctcaa
    34981 aaaacggcaa aatatgagga actatttttt ccgttttttt ctctaatact caggtcaaaa
    35041 cgctgatttc acaacctctg attcacggtg ttccagaagt agcttctacg gaaataagcc
    35101 gaaatttcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg
    35161 accacttctg tcacaacctc ttattttgat tagaacttgc gaaaataaac cggttaaagt
    35221 gtgtctagcg caagagattt gctataatag acgataaggg taaaatgtaa cgagaagggg
    35281 ttgaaaaatg tgtatcaaat tgtaacagat tcttgttgtg atctgccata ccatgtatta
    35341 gcaaaagaaa aagttgattt tttatcaatg aatctccaat tgaatgatca agagttagta
    35401 gatgacctcg gaaagacatt caattatgac cgctacatga atcagctgaa aaatggtgcg
    35461 atgcctacga catcacaaat caatgttggc cgatatttag aatttttccg ttcttatgcg
    35521 gaaaaacaga ttcctgtttt atatgtggct ttttccagtg gattaagcgg ctcttacagt
    35581 agtgcgcttc aagcggttga attgctgaag gaagaatatg aagaccctga gatctatgtt
    35641 tttgatacaa aagctgcaag tattggggaa gggcttttag tattggaagc tgtaagattg
    35701 aaaaaagaag gtaaaagcat cgaagaaacc ctagagtggc tggaaagtaa ttatttgaaa
    35761 gtccattcat gggtaacagt agacgatctg aaatatctag aacgaggcgg taggatttcc
    35821 aaagcaagtg ctactcttgg cagtctattg agtgtaaagc ctattattac ggtggataaa
    35881 gaaggaaggc tgacgaatgt tgacaaagtt cgaggaagaa acaagtcgat ccagacaatc
    35941 gtcaatgaga cggtaaaaac aatcgttgca ccacttgatc aaatcgttta tgtggcttac
    36001 gctggggatc tagagagcgc ggagaaggca aaacggcttt tggaagagca gataaagatg
    36061 aaagatgtga agctttatcc cttaggacca acgatcgcca gtcacacagg ctatggctgt
    36121 atcgcgattt tttcgatggg aatcagtcga taaaagcagt aactcattct gtaaaaaagc
    36181 ccagaaattg attgtaacaa aaacagcgac tcttaaaaga tagatgagca tcctatctct
    36241 tagaggtcgc tgttttttta ttttttaagc taaataggcc attatgccca taactcgttc
    36301 aattgttttt gaatatctgc attttccaaa aattcatcat atgtcgtatc agctcgatcg
    36361 atcacacctt tatccgaaac ggcaatgatg cggtttgcca atgtttggat aaattgatgg
    36421 tcatgtgacg caaataagat tgagccagta aatgccatca agccgtcatt caatgctgtg
    36481 attgattcta agtctaaatg gttcgttgga tcatctaata caagtacgtt agctttcgaa
    36541 agcatcattt ttgaaagcat gcaacggact ttctctccac cagataaaac attcactggt
    36601 ttcaatactt cttcaccaga aaacaacatt cttcctaaga agcttcttaa gaaggtgtta
    36661 tcatcttctt ctttacttgc atattgacgc aaccagtcta aaatcgttag atcagtatcg
    36721 aattctttcg ttgtatcctt tggtaaataa gaattacttg ttgtcacacc ccaacgtact
    36781 gttccagtat ctggcgtaat ttcacccatg ataactttga atagcgttgt tgttgttata
    36841 tcattttctg cgacgaaagc gaccttgtct tctttgctca aattaaaaga tatatcatct
    36901 aaaattttct ttccatcaat cgttacactt acattttcta cctgcaataa gtcgtttcca
    36961 atctcacgtt caggtttgaa tccaacgaat ggataacgtc tagaagaagg ttggatatcg
    37021 tctaaagtaa ttttttcaag cattttttta cgtgaagttg cttgttttga ttttgatgca
    37081 ttcgcactaa agcgggcaat aaattcttgg agttctttga tttgttcttc ttttttggca
    37141 tttgcgtttg cttgaagttt agccgctagt tgactagatt ccaaccaaaa atcataattt
    37201 cctacataaa gtttgatttt accaaaatct agatccgcca tatgtgtaca tactttgttc
    37261 aaaaagtgac ggtcatggga tacaacgatc acagtatttt caaaattgat caaaaattct
    37321 tccagccaat tgatcgattg gatatccaga ccgtttgttg gttcgtctag caaaagaaca
    37381 tcaggtttac caaatagtgt ttgtgctaat aacactttta ccttttgtcc accagttaat
    37441 tcactcattt gctgatggtg caattcttca gggatgttca gaccttgcag taaaacagct
    37501 gcttctggtt cagcttccca tccattaagc tcagcaaatt ctccttctaa ttcagctgct
    37561 cgaataccat cttcgtcagt gaaatcctct ttcatgtaaa tcgcatcttt ttctttcagc
    37621 acttcataca gacgcttgtg tcccatgatg actgtttcaa gtacagtata gtcttcatag
    37681 tcgtaatggt tctgtttcaa tacggccatc cgttcatctg gtcccatagt aacggaacct
    37741 gtggaaggct gcagatcccc tgataacact ttaaggaacg ttgatttacc tgcaccattt
    37801 gcaccaatca agccatagca gtttccgggg gtaaatttaa tattcacatc atcaaatagt
    37861 ttgcggtctg aaaagtgcaa actcacatca tttacagtaa tcaatttttt tcctcacttt
    37921 tatcattatt agttttgaca agtcttatag aattatatag gggaaaggta aaagattcaa
    37981 ggcaatcgaa cattctttgt gtttcacgtg aaacagaatg aaaaaaataa aaataaaagc
    38041 ggagcgaaaa tagctactat aggatttatt agtttgcaac agcataattt ttgacgttat
    38101 ttataagagg gagtactata aaactgtaaa agaaatgaaa gaaggattga aaatggatga
    38161 aaaaagaacc atcgactggg gctcattgat tttgggaatt ttatttgtac ttgtctcatt
    38221 gttatcattt caagaccctg tcggaaattt agttgcaatt gtagtagtct ttgcgatttt
    38281 tgcctttatt aaagggatat ttgaactgtt tgtacggaac agattaaaag agctaacagg
    38341 atataaagga aagatgccct taataatcgg gatcatcgat attctagttg gcgtcttttt
    38401 cttattcaat attggtgcag gagtcgcagc attgcctttt gtttttgcag tatggtttat
    38461 tgctgattca gttttagcgt tatttactgc tgacttggca agaggagtaa gtgaaggata
    38521 ttattggttt acgatcatcg tcaatatttt aggaatttta ttaggaattt tcttattgtt
    38581 taatccaatt tcttctgcgt tgacattaag ctttttagtt ggattctatt ttatgttatt
    38641 tggtatcaca catatcgtct atgcatttag ataagtaaaa tcaaacaaat aaaaaagccg
    38701 gcttccgatt acagttttac tgttgtaatt ggaagccggc ttttttatca tggacaaata
    38761 tgcacgaggt gatttttagg tgtcgagaca aatttatgca aaaatgtgag aagctatata
    38821 cacaaatttt atcttaatac tcgaagcata gcgcagttgt cacaacctct tttcatataa
    38881 ttcattcaat actggcaaat catggacttc atatttttct ttgaatgctt ggatttcttc
    38941 ttcagaatat tttcttggat ctaaatgcag atcttctact ggcaatgggc gcttgttatt
    39001 gatcttcaca tcgattacta caggacgatc tgattttgca gctgcatcaa aggcaaatcg
    39061 aagttgcgag tattctgtga ctgtaaagct atttgccccg agcgcttctc caactttacc
    39121 gaaatctgca tcttctaaaa atacaccaaa tttcttttgt tccgtatctt cttgttctgc
    39181 ttcgatgaaa ccaaaagaat cattcgaaaa gacaacatta ataattggaa gctgatattt
    39241 gacttgtgta atgatatctt gcatgaccat agcaaacccg ccatcaccac ttaaggtaaa
    39301 tacttgtttt tctgggtagc ttagttgtgc ggcaattcct cctggcacac cattacccat
    39361 agttgcgaac catccagatg tcgtatgttt ttgtttaccg ttcatattca ataaacgaat
    39421 agaatggata gtcgtgttac cgacatctgt gacgaaaatc gcatcgtctt cagcaatacg
    39481 attgatttct ttatagacag cctctggacg aatcggttct tcctctcgat cttcaaaaga
    39541 tttcaaccaa tttacccatt tttctttatt ttcttgattt gctaggtacc agctatcagc
    39601 agggcgagca tcccctaact cgacaagtct ttgcaaagct gtgtttgcat caccgaggat
    39661 cgacaaagat gtttcatggc gtcgtccaaa ttttgacgca tcgatatcga tttggataaa
    39721 ttcagcagtt ggattaaaaa atgcacgtcc aaatgggaag tcgctaccga caaataagat
    39781 cagatcggct tctgccaatg cttcatttcc gggtttagta gcgacacgtc cagcaaaacc
    39841 aagaaagttt tcataatcat caggaataat tccttttgct agaacggaag cgacaacagg
    39901 aactgaaaaa tgttctgaga attctttgat ttgcggaaat ccgtttcgcg ttccttgacc
    39961 aacataaaga actggttttt ttgctttttc aagataaggc aaagcagcta gcaaatcttt
    40021 ttcttctggt aatatcacac ttgttttgtg agtgtgagcg gtggagaaag gttgttcgtc
    40081 aatttcttca aaaccaagat caacagggat agtcacgata gccacgcctt tatgctcata
    40141 ggcggcttta atggcttcat ctactacatg cggaagactt tgtggtgtca tgactgtacg
    40201 attgtatacg ctaacatctg cgaaaatagg attttcattt agttcctgga aagagttata
    40261 gttcatggat gtagaagcaa cttgtcctaa gaacgctact agaggcacat ggtccatctg
    40321 tgcatcatac aaaccattga tcaaatgtgt cgcaccaggt cctgcagagc cgaagacagc
    40381 gccgactttc cccgttaatt ttgcatctgc agcggctgcg agtgcgccga cttcttcatg
    40441 acgaacttga atgtagttga tttcggtttt ttcttgatat agcgcatcca taattgaatt
    40501 gaaagatcct ccgggaatgc cgtaaatatg gtcgataccc cagctttcaa gaacttttac
    40561 cattgcgatt cctgcattga tttttgacat ggtgtttcac tcctttagca atgatgatac
    40621 ataaattttt tgtatctaga gtatagaact ttttatcgaa aatcccaact aattcgtctt
    40681 ttcacaaaaa tgtagtcatg tttttgggaa tatgctagag tagtacatag atagattcaa
    40741 gagaggggaa tggcatggtt gaatggctat tgagacttga accttggcag caagcacttg
    40801 ctgggacagc ttttacttat tttatgacag ctcttggtgc cgggttggtt tttttcttta
    40861 aagaaatcaa aaaagaagtg ctaaacttga tgttaggatt tgcttctggt gtcatgattg
    40921 ctgcaagttt ttggtctttg ttagatccgg ctattacaaa agcagaagaa aatggagata
    40981 ttgcttggct agttgtaagt attgggtttg gtttaggcgg tgtgttttta tatatggcag
    41041 ataaaacact gccccatatg cattttggac cacaacatga agcagaagga ttgcctaccc
    41101 atttaaaacg gacgatcttg cttgtttttt ctattacatt gcataacatt cctgaaggtt
    41161 tagccgtggg ggtagcattc ggtgcagcag caactgcgga taatccgaca gccgctatat
    41221 tagccgctgt ttcggtggcg ctaggtattg gtatccaaaa cttcccagaa ggagcagctg
    41281 tatcaatccc gttgcgacaa gaaggtctga gtcgtaaaaa ggcctttgtt tacggacaag
    41341 cttctggaat cgttgaacca attgctggag tgattggagc gctactagta acgaaagtgg
    41401 agctgctgct tccttatgca cttgcttttg ccgcaggagc aatgatttat gtcgtggtgg
    41461 aagaattgat ccctgaagca cagcagacat tgtccagcaa acgtcatttt gcagtttttg
    41521 gcgtcatgag tggatttatc attatgatga ttttggatgt ggcattagga taaataaaaa
    41581 aaatatatat gatatatcaa atagggccgc gaacttatat cgcgacccta tttttttagt
    41641 aagttatatc ataatgaagt gaatggtatc atacattaat aatagttttt tctatgtgta
    41701 tttcctttga tccagcgaac aagccagatg ataagtgcga ttgggattag aactttgacc
    41761 gcaaaccata aaagcccgct taggacactg acaaaaagac tgatgacgaa gctagctgca
    41821 attagcacta aaagaaccat gaagatattt aagaacatat ttttttgacg catacaaaac
    41881 cctcctcttg ttcattcttt actatagaaa aagtataaca gggattgttt aaaaacacct
    41941 ccaactgaag actgattttt acttcatcta cttaaatgat caaacaattg ctttttctct
    42001 ggtttatcat ctaaaataga gcggaaaagg aggagaatat catgcctaaa aatcgtcttg
    42061 aagcatttac agatgctgta atcgcgatta tcatgactat tttagtttta gaacttcatg
    42121 aacctaacgg agacacgtgg gaggcgctcg ctgcattagg ttaccgattt tttatctata
    42181 ttgtcagttt tatcatttta gcgatttatt ggaacaatca tcatcattta tttcaagttg
    42241 tacataagat aaatggacgg gtcttatggg cgaacagttt ctttgttttt gcgctttcgc
    42301 tttttccgtt tgcaacttca tggatcagtg agtacatcaa tagcctggct ccagaaatga
    42361 cttatggttt tgttaccttg accgctaata ttgcgtatta tatcttggca aaagaacttg
    42421 tcaaagcaaa taaaggccac gagaaagcat tgaaggcgat gttgaataat tacaagaaat
    42481 cgtatctatc gattggtttg aatgtgttag gaattgtctt agggtattta gtagcgccag
    42541 tagccgtgct gatcgcaaac atcgtgattc tagctgcttg gatcatccca agtcgtacca
    42601 tcgaatcaca atatcggtaa aatgatccgt gtttaaaatt taagagaaaa atatagaaaa
    42661 aataaataaa cgatcaaata tccgagtgat actagtattc aaagagggat gcagatggat
    42721 caaaaaagaa ttcatgtagg actgattact agttttgtca cgatgcttat ttatttggat
    42781 caaggtcatt tgacttggac agtagttggt gtcgtaggat ttttgacatt attcacagca
    42841 ataaaaaaat aaacatcttt atgtaatctg atgttctatc tatttaagaa aagcatgttt
    42901 acagcagtat gattctgttg taaacgtgtt tttatttgtg gaaaatttaa tttttagaaa
    42961 agtgtatcga aaaatagata gttagttttt ccacagtgga taacttcgta agagataaaa
    43021 ataaaaaatt agtcaaagaa gggcgtatag atttcttttg ataggagata tacaacagaa
    43081 atgaataaat attaacgtgt tttcaaacag tataaaacgg tgtggttttt ggaataaaaa
    43141 aataacatat ctcattttac acttttgtta ttttatactt aaaaaataac gacacagaaa
    43201 ataccgtgtc tcagatttct ctactacttc taaaaactac tgtttttttc ctttgaaatg
    43261 ccttcaaaaa gtaggatcag gcagaatata ctggcagaat caaggagaag ggagcctaaa
    43321 taaggagaaa aaccatagaa aatataaagt aattacttat aaaacaagct ttttatagaa
    43381 aaataatata cctgagtagg gtataaatta agacggaatt cctttctaga ttgagatatt
    43441 gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct ttttgttaaa
    43501 taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa taatatagta
    43561 gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac cgtcatagat
    43621 acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg aagacggaca
    43681 gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta tcccaaaata
    43741 atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg ataggcccat
    43801 aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc tacgccaata
    43861 ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat ccctaatttt
    43921 ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg aaaaaagaca
    43981 atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa agtagaaaaa
    44041 gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt aaatgatagc
    44101 ttgaataaaa tcaaccataa tagaaagaat aaataaatcc ctaataaaaa ttttgataca
    44161 taccgcatag acatctcctc catttaaaaa gtataccatc gaaaaccgct gaaaaatcat
    44221 aaaaaagtat gaagtttaag gaccaacgta gttatcgtac gaaaattaga agcttttttt
    44281 gttgggaatc cggtttcttt aacaaagcta agatagcgag ttgcttggaa aagttgctat
    44341 cttttttctg tcaaaacgac tcggaatgtt cgttttttgt tctaatttct ccaagttgtt
    44401 tactttttga tactaagttt atgaaaaatc aagaaatagt tgttatacta ggtaatgctg
    44461 acaaacggga ttggcatttc atcttggttc agcttttttt ccaattacta ggaaggaggg
    44521 atagattgtt caagacttat tttcgtttca ataaaataca agataatcct tttcgtatca
    44581 tgaatctgat gctacttatc tttattgtat tatggttagg ttacataaat catgattttt
    44641 tgatttcaag tttttcatca ttagggatct ttactttttt ctattatcaa aacataccga
    44701 tgaaatattt gctgaaacgt atgtttctga tcggttgcgg actgttggtt gctttcgttt
    44761 tggggatctt atcgacttat gttttatggc ttgaaccatt tgccgtagct ttggttgctt
    44821 tttccagcag attcgtgttg cgattattcc atatttctaa accaggtggc ttgttttttg
    44881 cgatgctttc tgccatggga acgagcatgc agctgccaat cgctcaatta ccgatcgtct
    44941 cactttattt ttttatgggt gtcgtttttg ccctgattgc ggcagtgatt acaaaattac
    45001 tggattcacg gcctgagcag acaattgaaa aagcaacttt aaaagaaaga tttcacgaag
    45061 agcctttagt gatcattgat tctgttttct acagcgctgc cttatttctt tccgtttatg
    45121 tcagtcacgg cttgaatctg cataatcctt attggttgac cttgtcatgt gcatcgattt
    45181 tgctagcaga aaacttggat gcgatgaagc atcgacaagt ccaatatctg attggatcga
    45241 tgggtgggtt atgtgtgtcg gcattcttgt catttgtgcc gtttacgcag ttgcagacca
    45301 tttttctgat cacttttcta tatggaatcg cacaatttct agttgctaga aattatgcag
    45361 ttgctaatat ttttcttaat ccgatggcgt tgatgctatc tacacttatt cgtggagcat
    45421 acttgatttc tttgatcgaa tatcgttttt tagggatcgt cataggaagt ttcatcggtt
    45481 taggtgttgc gtgggtaatg acggttggtt tgcaacatta tttaacggtt gttcgtcaaa
    45541 aattagaata gaaggacaaa tatatgtgcc aaaagaggtt atgacaagat ttttgtcaca
    45601 acctctaatt caaggtgttc aaaagccaga ttctcagcat taagtcaaat tcacacaaaa
    45661 atgtggaaag gaactttcaa aaaaatttta tatttattct atcaaaaaat tttgtttatg
    45721 atggtaactg aataaacttt cctgagatgg tcaatgcttt ccttgaaagt cttacagata
    45781 ttctttcttc atagaaagtt taagttttat agttagtgta caataaaagt atgtttatac
    45841 catatggtgt ccggataatc agtcatttaa acaatgaggt tcaaagatat gaaaagggga
    45901 aatttgatga agaaaataag caatgaaatc caaattctgg cagaaacttt aactcttccc
    45961 aaagtcgtta tagagcaaat gattgtacaa gcaaaaaaca tccttgatga ggtgtttgag
    46021 caatatagtc atgaactaat ggatcctaat aaagcaaagg cgactgcaaa gaaaatagac
    46081 aaagtctatg aagaggaacc ttttgcagca ttagctattt atctatatgc agcttctcaa
    46141 agttggaaaa aaatttatca accattgaag attccaagaa ctatctattt ggcaacaatg
    46201 aatgctttta ctagatttat tcatgaacat ttccaagtaa caggaagcta tcgctttgac
    46261 cgtggttttt ggatttggca ttacactagt ggcttgatct ttcggattgg tgaattggag
    46321 tacgaaatga catattttcc gaaagggtac acggatctaa gattagaagg aaagcagtgc
    46381 ctttctcttc atattccttc cgatgcagat ttatctcaga ataagataag agatagttac
    46441 gaaagtgcca ttcgtttttt tgaactttat tttcctgatt accattacca agtgatgtac
    46501 acagatactt ggttactatc acccaatctt actaaatggt tgaaaaaaga gtcaaaaatc
    46561 tgtctttttg cagccgatta tcgattatta tcagttgatg aacaggacga tagtggagtc
    46621 ccttggatct ttggacgagt agatgcccaa atacatgatt atccagaaag tacgagttta
    46681 cagcgacaag caaaagagca attgcttgca ggcgagcaca ttggctctgg cttaggtatt
    46741 cttacttcat aagtttagta cttcgaaata gtttaaaaga gcgtgagaca aaagtaaaga
    46801 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcagaagca actccttcgg
    46861 aaataagctg aaattcacaa aaatttgaaa agcaattttc gtgaatttct tcttatttct
    46921 tggagttaaa cgcttctgtc ccagcctctt ttttagtgtt ctatcgaaca cacagtacgt
    46981 tcaataggta aactagctat aacaatagat attaaagtta acggacaaga gggccttttt
    47041 taggtagcta aaacgtaata atgaaaattt atttccagag ataggagtca atcagctagt
    47101 ctgatcgact gttttaacaa gttgactttc attttaactt ttgataccat ttaaaacaca
    47161 aatcgtaatt gtttcgattt gtgtttttta gcatgactac aatcaaagaa gaaagtgggt
    47221 cgtccatatg gaaaaaacag atatctcaag tgcataccgc cgcttgaaaa gcccaaatat
    47281 caaaacacga aaacgtgcgc tgaagatcat caaagatgtg aaacggaata gtgggaaaag
    47341 gtaagaagaa ggaaacttaa ttaaaaaaat agccgaaaaa cgtttttcgg ctattttcaa
    47401 taatcggttt ttgtgtaagg tttccgaaag aactgctgtt gattttccat ttttaacata
    47461 tgataaattg gtagaatctt tcttttcaaa ttgataccat tccctaattc cagttatata
    47521 gttaactaca gaaccacaaa gcacgtacgt ttataaaagt attatctgta gttatttttc
    47581 aattcccttt caacctctta ttttgaccag tagtattgat cactttacct tttaacacct
    47641 ttgttcgttt ttgaaacatt tttagtcaag aatggaaacg taactacata atcaaaggtt
    47701 ttgcgaaata tttcatgaaa tatttcatgt agaagcgagc aatacggttg caaataacca
    47761 ttatcgttta ataagcagtt acgaaagctc ccaatatctc taacgttcag ctcactccat
    47821 ttcttttctt caaaaaaatc agtgattcgt atctctttct atttgtcgaa aaagttaact
    47881 ttaaaaagta acgccgaaaa aatatgcaaa aaaataaaaa agcagttact attgctagaa
    47941 taacatcaag gaataataag ttttggtttt cagataatag tcgataagtt gtgtattgga
    48001 attttagata cagtaataaa gtatttttca tgaaattttg tgagaatcac tattacaagt
    48061 atctttaata aaattaatgg ttaaggagga acttaaagta gaatgtaaat gaatagttgt
    48121 agtcagtcga ttgtgatact agactcaaag ttgcaggaat aagtttttca atttgtgatt
    48181 ggaagataaa aaatgaaaaa gaagggcttt tttttatact ttgttgattg tagtgtttat
    48241 tttccctctg tttagccaag taacattttc tatgactcaa ccagtttcaa gtgaggaaca
    48301 tgataacctt tttcgtgcta cagaagagtt taagaaatat caggaaaata ttgttccgga
    48361 gactccatta gctagtgagc caattactga tattgatgga aaccaaatag gatggtatat
    48421 acagtacgaa gttatggtgg taaataatga ctcaatagta aaaaatacag taggcttaga
    48481 gctaacaccg attgaaaagg tgagttcatt gttaacgtat atttatactg atgaaagtga
    48541 cgagactgag atcattttaa cggattactc aaaaattgct actgataaaa agtatatatt
    48601 gtggatgtag acactagtga aatactttat gatatagaaa ttgatgaaaa tttactagat
    48661 gtagcacaag aaatcgatca tcaaaagcag gaagctattc gatatgctgg tagaaaaggg
    48721 aaaagaaaat atttattacg tgaatgtagc aaaagttaga gaagatgaaa atgagtggaa
    48781 agaattcaag agtcgctact caattaactc gactccgaca tttacagttt acagagaagg
    48841 atcgatagaa aaaactgttt tttggactaa agaaagtgga atgtctttag ctgaagtaga
    48901 agagtttttg gattatgtta gtatgcaaca gtaagggagt tcttattatg aaaaattttg
    48961 acacatatta tgttattgtt ttttcgatat ctttgctgtt aatattacta actttaatgc
    49021 agtataaaga gtatccagag tattttattt taacttttgt atatttagta agtagctaca
    49081 cacttctata taaattcaga agttcttcta gagtttctaa agtttgtttc ttaattagct
    49141 tattgattct ataatatttt tgatttatat aggaaattaa aaatattata gaatcaacac
    49201 tcagagaacg ggttggtttt taatagattt aataagtact gataatgaaa tttctcctgg
    49261 ttgggaaaac gaagttggtt accctgtaga agaatttttt caagattact ttgcggagta
    49321 tgccgatagg ataatgagag attacgtaac agataaaact caagaagtca tcaatcaatc
    49381 ccagtcatta aatgaattat ctgataaggc gaaagaagaa gcagttgcca ttatttcttc
    49441 agggattagt gactatattg atatgagtgg tgaaatcaat tatggcgata aagacaaaat
    49501 tgtacgcatg ataaaagctt ttatcaaaac ttttgatgtg aaggcagatc ttaagtttaa
    49561 tgatacttac ctagtaggaa aattaattaa tatccttggg acagagaaag atacggaaac
    49621 gtttatttcc tctttgtcta tgttgtttac agataagcat ttttcaactc agcttggtgg
    49681 agaggaacta gtggaaatct tttaaccatt acgcccagca ttaataaaag tatatcaaaa
    49741 aagtactcct gatgattttt aagattggtt tgagaaataa tgtgaaaaca ctatttttaa
    49801 tgttaattat agatagagat gaacgcgtca tatggttaaa agaatgaaaa agtcaaagat
    49861 acactgctat atttcactaa ggatatagca gtgtatcttt catgtcgttt cactggcagt
    49921 tttttataaa aggaggtgtg catttcagtt gcttttcttt ggtagttagt tgaatcgttt
    49981 aagaattaac ggatgtggca gacaggtgct ctgtccaaaa accagtaaaa tctttgatgg
    50041 ttccatcttt atttagctgt gcctgttcaa atactgtttt gtttttaccg aatggatcaa
    50101 ttgcaataat tgttccgatc cccgcacgtt tggcagcaag taatcctgag taagcatctt
    50161 cgataacaag acaatcagcc ggagctaagc tcagtttgtc tgccgcttta agaaaaatat
    50221 ccggttgcgg tttcccaggg aaagagccgt cgtcatagac aatcttgtcc cagtcaaacc
    50281 agcgggctaa atcgaaaata tcaaaataaa aagcaacatt ctctttgaca gtagccgtgg
    50341 caatcgttaa tggaatctgt tggtcagtca gctggtcaag agtcgcttca agcccttttg
    50401 tcaactgaag ctcgttttca ttttgcaagc aaagcatacg ataatagctt tctttttctt
    50461 ctgacaatac ggcgatctca gaggcggtca aatgctctga aatgaaatgt ttcaaaatct
    50521 ccgtattcgt ccgaccatga atatttttta aaatatcctc gtctgataaa ccatcttttg
    50581 tatgatcatg tatcatatgc agccaagcag cttcatgcag atgagagtcc aaaaacattg
    50641 ttccgttgaa atcaaatata atccctttca tagttacgct cctatagtgg tattttcggt
    50701 tgaatcagca atttgcagat cgacatattg tgcatagtgc tcttgaacct cccgcgagat
    50761 ctggcgatcc gtaatgagca gatcgaaatc atgcaaatta taaaacacat aaaaatcatc
    50821 tcgattgaat tttttattat caacgagtag atatttctta cgggaattat ttagagcgat
    50881 ctgctgtgct tctccttctt cggtactgta agtagagatc gcttcgttgt gaacgccatt
    50941 tgaactgata aatgcttttg taaactttag tcttcttaaa ttgtcgttgg tgatcggtcc
    51001 gacaaatgcg ccggtatttt tccggaaatc accgccaatc agtagaattt ctggtccatc
    51061 tgcttgagca agaatatcaa aaactggata attatttgta atgatccgaa ttttcttgtt
    51121 cagtaaatgc gtcgccaata gttcgattgt tgtacctggt cctaagaaaa tcgtttctcc
    51181 ctcgtgaatc aaggaggctg ctgtggcagc aatttctttt ttttcttgaa tctgcagagt
    51241 ggctttttca gtgtgagaaa gttcttgatc taaagtgtag gaaagacttt gagcccctcc
    51301 atggacacgc agcagcttgc ccgatttttc taattcatct aaatctctgc gaacggtcat
    51361 atcagaaaca tctaattgat tgatgatttc attaacggta aggattcctt ttttattgac
    51421 catttctacg atagtaagca atctttcttt tttcaacatt gtcttcacct ctaatttaag
    51481 gataatctat catataaacg gacaacagtc aatctaattc gaacaaaagt aaacatttag
    51541 ggtggttttt atgtttgtta caatagattt tattgctgtt ttctggtttt tatgattatt
    51601 tgtgtttgtt tttgttgaca aatgtttcgg atgcgtttac aatgcaatca gttagaaaga
    51661 gcgaaaactg attatagatt tgaggagaga tgtacatgct taaattacca gaagatttta
    51721 tttttggcgg agccacagca gcctatcagg ttgaaggagc cactaaggaa ggtggcaaag
    51781 gcgctgttgc ttgggatgac tttttagagg aacaagggcg attcagtccc gatccggcaa
    51841 gtgattttta tcatcaatat gcaaaggaca ttgaattgtg tgaacgtttt ggtgtaaatg
    51901 gtctaagatt atccattgct tggagccgca tttttcctga tggagctggg aaaccaaatc
    51961 cagaaggaat cgctttttat catcgtgtgt tcgaagaatg taagaaaaga aacgtaacgc
    52021 cgtttgtgac gttgcatcat tttgataccc ctaaacgttt gtttgaccat ggagattttt
    52081 tgaatcgaga aacaatagaa gcatttgttt cctatgccat tttttgtttt catgaattca
    52141 aagaagtcaa ggtgtggagt acctttaatg aaatctatcc agttgcaacc aatcaatatt
    52201 tgttaggtgt tttcccacca ggaatcaaat atgattttac aaagatcgtc gcctgtcttc
    52261 ataatatgat ggttgctcat gcccgtgtag tgaactattt caaagaaaac gaacttccgg
    52321 gtgaaattgg tgtagttcac tcattagaga cgaaatatgc ggcaacagac gcgccggaag
    52381 ataagcatgc tgccttttta gacgatgcat tgtcgatccg tttcttatta gatgctacct
    52441 atttaggcta ttattcaact gaaaccttga ctgctcttga tgagatatgt gaagcaaatc
    52501 aggccagcta tcattttcct gaagaagatt tcgttgaatt gaaaaaagca tccacgcgta
    52561 atgactactt agggatcaat cactaccaat gccattttgt caaagcttac gatggagaaa
    52621 atgcaatcca tcataacggt acgggtgaaa aagggacctc tgtttataaa gtgaagggaa
    52681 tcggagaacg tatttataaa gaaggaattc ctcgaactga ttgggactgg ctgatttatc
    52741 cagaaggtct atatgacttg ttgctgcgaa ttaaaagcga ttacccacat tacaacaaaa
    52801 tctatatcac tgagaacggg atggggtata aagatcagtt tgaagatggc attattatgg
    52861 atcagccgag aatcgactat ttgagagtct atttggaatc cttaagcaag gcgataacag
    52921 ccggggtaaa tgtgaagggg tattttttgt ggtcgcttat ggatcttttc tcatggacaa
    52981 atgggtataa caaaaggtat ggcttgtttt atgttgattt tgaaacccag aagcgttacc
    53041 caaaagaatc agcttactgg tacaagttgg tcagtgaaac caaaacgatt atttaataag
    53101 tgataatgga ggaagaaaag atgaataaag aggaattaca aatgcttggt tttgagatcg
    53161 ttgcatattc aggcgacgct cgcagtacat tattgaaatt attgaaagaa gtaagacaag
    53221 gaaactttga acatgttgat tcggcgttga aagaagccga cgaaaattta acccttgcac
    53281 ataattcgca aacaaaaatt ttagcggaag aagcatctgg gaaagagatg gagatgggat
    53341 tcatttttat ccatgggcag gatcatttaa tgaccacttt attgctgaga gatctgattc
    53401 aagactttat tgtactgtac agacagaatc aaggatagga gaagaacgct atggatggaa
    53461 tcattaaaca aatcgaaaaa gggaaaccat tttttgaaaa aatctcacgt aatagttatt
    53521 taggagccgt tcgtgacgga tttttaacag cgatgccagc tattttattt tcaagcattt
    53581 tcattttgat tgctgcggtt cctgagattt ttggttacca atggcctgaa tctgtctcca
    53641 catggctatg gaaagtctat aattattcga tgggtgttgt agggttactc gtttcagcaa
    53701 ccactgctcg ctgcttggct gattcgatga atcgtcgctt accgaaaaac aaagtaatca
    53761 acaccacctc tgtgatgctg gcctctgtgg taagtttcct gttgttaagc gtctctccga
    53821 ttgaaggtgg attttcaacg gaatatatgg ggacaaaggg actactggct tcctttgtcg
    53881 ctgcattttt aacagttaat ttatataagt tttgtgtgct gaaagatatt accattaaaa
    53941 tgcctaaaga agttcctgga acgatttcgc aaaccttccg agatattttt cctttctctt
    54001 tttcagtatt tgcggccgtt ttaattgatt taggcattcg acaaatcttc caaatctcat
    54061 ttgcagaagc aatcattacg gtgctgcagc cgctatttac agcagctgac gggtacttag
    54121 gaatcgctat tatctgggga gcgatggcgc tgttttggtt tgtcggtgtt catggaccgt
    54181 cgatcgtgga gccggcaatt gcggcaatta tctatgccaa tgtggaaacc aatctgcaat
    54241 tgttcaattc cggtgaacaa gcttctaaag tactgacggt gggtttaggg aattttgtag
    54301 gaactatggg aggaactggg gcaacattgg ttgttccttt tatcttccta ctctttgcta
    54361 agtctaagca gttgaaggca gttgggaaag cttcctttat tccagtaagt ttcgctgtca
    54421 acgaaccatt gctatttgcg gcacccatga ttttgaaccc ttattttttt attccatttt
    54481 tgttaacacc tgttgtaaac gtttgcctgt tcaagttttt tgtcgatgta ttgggcatga
    54541 acagctttat gtacgttttg ccttgggcaa ctccagcacc gatcggtttg gttctaggaa
    54601 cgggcatggg tatcttagct tttgttctag cagcggttct aatcgtcgtt gacttcctga
    54661 tctatctacc attttgcaaa gcatacgatg ctgtcttagt agagaaagaa gcacaaaaag
    54721 cagaggtgat cgcaaccgag gaaggtgctt ctgccgatgt acgtgaggaa gtacctgtgg
    54781 cagcgagtca agttatcagc gatgcaaata aagaaatcaa tgtgttggta ctttgtgctg
    54841 gcgcagggac aagtgcgatg ttagctaatg ctttaaaaaa aggtgcatct gaatcgaaag
    54901 caccgatcag tgcaatggcg ggtgcttatg gttctcatta cgaaatgctg caggattttg
    54961 atatggtggt cttagcaccg caagtcaatt cttactatga tgatatcaaa gaagacaccg
    55021 atcgcttagg aatcaagctg gttgcgacaa aaggagcaga atatatcgga ttaacgagag
    55081 atcctgaaaa agctgttcag tttattctga accaatttca ataaaggagg agccataatg
    55141 aaaatgggaa tttggttgcg taaagctgtt ttatttgcag ctgtgttagg ggctttgggg
    55201 gctgtttcaa aaactgttga agcatctgcc agcgaattta ttcgcggagc agatatttcg
    55261 atcctgtcag atatggaaaa atctggtgca aaatattatg aagacgatat ttcaaaagat
    55321 gccttagaga ttttaaaaaa taatggtgtc aactatgttc gactgcgttt gtggcaggat
    55381 ccttataata gtaatggaga aagttatgga gcagggacaa atgatctgga aacgacgatt
    55441 gaccttgcaa aacgagcaaa aaatctggga cttaaggtgc tgctggattt tcactacagt
    55501 gatttttggg ttgatcctgg gaaacagaat cttcctaaag catggcaagg tctgaccttt
    55561 gaagaaatga atacggcatt gtacgactat acaaagaacg ttctgtcgga aatgaagcag
    55621 ctggacgttt atcctgatat ggttcagatc ggcaatgaac tgaatagcgg aatgctctgg
    55681 ccttatggca agtcttgggg agaaggcggg ggagaatttg atcgattggc tgcttttttg
    55741 aagtctggta tccaagctgt tcgagacact caacctaaaa caactccagt aatgctccac
    55801 ttagctgatg gtggcgatac aggagcattt acttggtggt ttgatgaaat cacgagtcga
    55861 ggtgtgtcat ttgatttaat tggagtgtcg tattatccgt attggcacgg ttcaatgaat
    55921 gaacttcaaa ccaatatgaa tgcgatcagc caacggtatg gtaaagaagt gatcgtagtt
    55981 gaaacagctt atgggcatac aacagcgaat gccgatacta tgccaaatgc ttttggtgaa
    56041 gccgaagcag ctgcgggggg atatgaacca acaccgacag gacaagcaga gtatttgctg
    56101 gatcttgccg atcggattca ggcagtaccg aataatcgcg gggcgggctt cttctattgg
    56161 gaacctcttt ggtacaacgg aaatgtgagt tgggcaacac aagcaggtat gaattatctc
    56221 agtgttcaat ccactatggg aaacgaatgg gacaaccaag cgatgtttga ttttgctgga
    56281 aacgccttgc caagcttgcg tgcgtttaaa caagcagggg cgcaaataaa tctagttaaa
    56341 aatgcaagtt tcgaaagcga tggaccaacg accagtcctt ccaattggca aacctggttt
    56401 caacaaggaa ccgctaaggg tacgatcaaa acggaagcgg gcactttttt tggggactac
    56461 aaactaactt tttggcagga tcaagcatac gaggcatcag cttatcaaaa tgttagtggc
    56521 ataagcaaag gaacttacac attgagtgcc tatgtaatgt ctgggggaga gtttgatact
    56581 aatcagttat atatcaaaaa ttatggtgga gcggaactaa atactgcggt tgttaaaaca
    56641 agtgaatgga caaaaattac aatcaaagac attccaatta caacaggtgt ttgtgaaatt
    56701 ggggtttata gcaaggcttc aggaaatgct tggacgaacg ttgattttgt ttcattggtg
    56761 aaacaataga ctgcggatcg tttcgattca agatcggagg ttcccttcag ccacatcgtt
    56821 agcgtagaag gcaggaatcg aacctgcacc tcgcctttgc caaaaataac cagaaacgat
    56881 ttccagtcat ttttaccaaa acgagtaaag caccagactt catctacact ttgtattatg
    56941 ctaaagagag ttcaacgatt tcgtaagaag caatgaaaaa attgcaaaaa gttgtgcaga
    57001 taacataaag tttgtttaag aaagggtaac cttagaattc cgtcgtgaat atgatttgag
    57061 cacttagatg gatagaaaaa agttctcgga aaacagttcg tttttcgaga acttcttttt
    57121 atgataagaa tgaaacaaat taacttatat tatagtctct tttggtaggg aaccatctta
    57181 tttttaatga aaatcaagca ttttagctat tgaagaatgt tgatttatag ggattccccc
    57241 tccatattat gttggctctt tattcccact ccactgttgc tggtggttta gaagtgatgt
    57301 catacacgat acggttgacg tgggctactt cgtttacgat acgtacagag attttttgca
    57361 agacttccca tgggatacgg gcaaagtcag cggtcattcc gtcgattgaa gttacagcac
    57421 ggataccgac tgtgtagtcg taagtacgtc cgtcacccat aacaccgaca gagcggatcc
    57481 ctggaagaac agtgaaatat tgccagatat cacggtcaag tccagcagct gcgatttctt
    57541 cacgcaagat agcatcagat tcgcgtacga tttctagctt gtcttcagtg atttcgccaa
    57601 gaacacgaat accaagacca ggaccaggga atggctgacg ccaaacgatt gaatcaggca
    57661 tgcctaactg agtccctaat tcacggactt cgtctttgaa taatgtgttt aatggttcga
    57721 tcaattcaaa ttgcatatct tctggcagtc cacctacatt atggtgagat ttgatcgtct
    57781 gtgctgtttc tgtaccagat tcgataacgt cagtatacaa cgttccttgt gctaggaaag
    57841 agacgccttc ttcaccagca agttttgttg cttcgtcatc aaaaacatat acgaattcat
    57901 tcccaatgat tttacgtttt tgttctgggt cagatacacc tttcaacttg tctaagaagc
    57961 gatctcttgc attgactttg atgatgttca agccgaattt tccgcctaga ctttccatta
    58021 cttgttccgc ttcgcctttg cgtagcagac cgtggtcaac aaagatagat gtcaattggt
    58081 caccgatcgc tttttgtaaa agaaccccaa cgacactaga atcaacgcca cctgaaagac
    58141 caagcaagac ttttttgtca ccaacttgtt cacggatttt ttctacttgc atatcgataa
    58201 agttatccat tgtccagtta ccttcacagt gacaaacatc aaaggcaaag tggcgcagca
    58261 attcgattcc gtattcggaa tgacgtactt ctgcatggaa ttggatacca taaaaattac
    58321 gtgttgtgtc ttggatagaa gcaatcggac aatctttgct tgttccgact gtttcaaagc
    58381 cttcaggaac tttagttacc aagtcaccat ggctcatcca tactgtttgt tgttttggtg
    58441 tttctttaaa caagacagct tgatcgcttg ttacttctaa caaggctttc ccatattcgc
    58501 ggtttttagc aggctctacg gaaccgccaa gtttgtacgt gatcaattgc ataccatagc
    58561 aaatccctaa tacaggaata cctaaattga agatctcagg atcgatccca aatgcattct
    58621 catcatatac actgtttggt ccgccagaaa gaatgattcc ctttggattc atcgctttta
    58681 cttcttctgc agtaatgcgg tggctcaata attcggaaaa gactccaaga tcacgaatac
    58741 gtcttgtgat caattggtta tactgactac cgtagtctaa tacgataatc ttttcaacgt
    58801 tcgtcatatc ggcaacgttt gtcaaattta ttacccctat ccctttaaat tttatctcaa
    58861 aatagtcaaa ctattttaaa atgctaatat tttcgcatga aaatcgaatc gatgatatga
    58921 ttttcagttt tatggagaat tatatcggct cttcctcgtg taggcaagat atattcctgt
    58981 aaatttttca aattcacatt tttccataca tcttttgcca ttttaaaggc ttcttcacga
    59041 tctccgatag caaaacgata ataataattc tctggatttt ggaaagccgt atccagtaac
    59101 gcaccaaaac gatccaaata ccatttttcg atcagcacgg gatcggcatc gacatagaca
    59161 gaaaaatcaa aaaagtcgct gacgtagatt tgctgattcg caggtaattg aagcgtattg
    59221 atcccctcaa cgatcaagat atcaggctgc tggatcaatt cgtacttgtc tggaatgata
    59281 tcatacacat catgtgagta aacaggtact tttatctctt cctgaccaga ttttacacgg
    59341 ttcaagaaat cgatcagcat ctccatatca taactttcag gaaatccttt acggtccatg
    59401 attccacgct cttttaatat acgatttgga taaagaaatc cgtcagtcgt gatcagctga
    59461 acgtttcttc gtttgaaggt acgagacaaa atcgtctgca gcagtctggc tgttgtactt
    59521 tttccaacgg ctacacttcc ggcgatcccg ataataaaag gcggtacttc tacgtattta
    59581 tgaagaaata aacctttact caagaacaaa gattcaaatt ctttcatata aagataaatc
    59641 agatgagcaa gaggtacata gatatctcga acatcctgca tggaaatgcg gtcattcaaa
    59701 cttttgatat tatctagttc atcttgtgtc aaaggtgctt gcccattacg ataaaattct
    59761 cgccattctt ctcttgccac acggtagtag ttcattggat cattcatatt agcctcctgc
    59821 gttctcccat caatggaact attttaacac aaaataaacg caaaccccac cacttaagag
    59881 agattgccga tctttttgta taatctttat aatagaaaga aacggagtct tattcttgac
    59941 ttgcgcacgg atacataaaa ggatagactg ttgtcatgaa agaaatgaga aggatggaag
    60001 acatgacaaa tcattactat tctgaacgcc cagatacggc gcacgatttt gaacaatggg
    60061 cttttgagtt aaaaggcaga acctttcaat ttgtaacaga ttctggtgta ttttcacgtg
    60121 aaacagtgga ttatggttca cgtgtcctga ttgatgcttt cgattgggaa gagttacctg
    60181 atgaaggaaa gatcttagat gtgggatgcg ggtatggacc gattgggtta gctttggcat
    60241 ttgcgacaca gcggtttgtt gaaatggtcg acattaatga acgagcggta agtctagcgc
    60301 aaggaaacgc aaagcgtaac caaatcgaac atgttgatat ccatcaatcg aatatctatg
    60361 aagcagttca tgaagaaacc tatgcagcta tcgtaagcaa tccgccgatc cgtgctggaa
    60421 aaaaagttgt acatgaaatt ttgacaggcg catatcctcg gttgaagaag ggcggtacac
    60481 tgacaattgt gatccagaag aaacaaggag cgccgagtgc gcaaaagaaa atggaagaaa
    60541 catttggtaa tgcagaaatc gtcacaaaag ataaaggata ttatatcatc aaaagtgtga
    60601 aagaataact agctgaaaaa tagtggagcg acgaaagagt ggaactgggc gtgcatgtac
    60661 gggtacgtat cagttctttt tttgttgaga agtttttaaa aacaaagaac tattttttct
    60721 tcaaaaagaa atagtaggaa cagagtcgtt gttttttacg ataaaataaa taagatacag
    60781 aacttgtata gaaggctgcc aagtcggtag ataaaggagg tttttatcaa aaaacatact
    60841 ttcctttttt ctctattctt ttcttggggg ttttggtaca ctgataggga atttagatag
    60901 aagggagcca ataggatgaa gctgaccatc agagatatcg cagaaatggc aggcgtatca
    60961 gtaacaactg tctcacagat tttgaataac aaaggaagca gatttagtga taagacaaga
    61021 aaaaaagtgt tgtcgatcgt gaatgaacat cattataagc ccgattattt cgcttcaaat
    61081 gtgatcaatc gacattcaaa gaccatcgga atgatcgtcc ctgatgtcac tgatttcttc
    61141 ttctccaaac tgatcgaagg tgtagagagc tatttgaatc ctttgggcta tgtgatcatg
    61201 ttatgcaatt ctcgtcatag ccaagaaaat gagatcaagt atttacaaga gctttcccac
    61261 cgctcagtgg acggaatctt gctggccaca ccaaatattt tacctgagaa atatgcatta
    61321 gacagcggtt tttatcaaaa aatgccaatc attttaatcg atcgcggatt gaatcgtcgg
    61381 gataacggac ggcttatcgt aaaggaatat gagggcgcct atcaagctgt ttcgctgttt
    61441 attgaaaatg gccatacgaa gatcggcatg ctgaaggaaa ccactggata ctatcaatta
    61501 gaagaacgtt tcaacggata tcgtcatgcg ctgaaagatc atggcctgcc ttttaatggg
    61561 aaatttgttg aacatggaga gttgactgtc caaggtggat atactgcatc aaaaaaatta
    61621 ctcaaacata aagatatcac ggctattttt tgcgggaatg atgccatggc gatcggatgc
    61681 tatcaggcga tcgatgaatt gggcaaaaaa atcccagaag atatctcagt cataggattc
    61741 gatggattaa agctgtcaga atacatgatt ccgcaattga cgacagtgca gcagccaagt
    61801 tttgatattg gattttatgc tgcacgtttc ctgatcgata cgatcgaatt tcctcaaaga
    61861 aaagttccga acaaagtgtt cgaaacaaaa ttaattattc gtgagagcgt aaaagtattg
    61921 acaaagggct aactcggcgg tatattgctt atgtaagcat ttacaaaggc tagttagtgc
    61981 tttttttatt tttagtaaac cagtttacta aagtggttta cctttcctaa aaataatgta
    62041 taataccgaa tgaagacaag gaggatcagg caatgcgaat ggtggatctg attgaaaaaa
    62101 agagggatgg aaacgagctg tcgaaagagg aaatcgagta catcgtcacg aactatacga
    62161 atggaaagat tccagattat caagtcagtg cattattgat ggcaattttt tatcaagaca
    62221 tgacaaatga agaaatcacg aacttaacat tggcgattgc taattctggt gatgtcatcg
    62281 atttgagttc tcttgaagga ataaaggtag ataagcattc gacaggtggc gttggtgaca
    62341 cgaccacatt gattttagcc cccctagtag ctagtgtcgg agtgacagtg gctaaaatgt
    62401 ctggacgcgg tctaggttac acaggtggta cactagacaa gctagaagca attcccggat
    62461 ttcagatcga gctttcggat gaagaatttg tacgcattgt taatgaaagt aaagtagcgg
    62521 taattggtca atcaggaaac ttggcaccgg cggacaaaaa attgtatgcc ttgcgtgacg
    62581 tcacagcgac cgttgattct cttccgctga ttgccagttc gattatgagt aaaaaaatcg
    62641 cggcaggagc agatgcaatc gttttggatg tgaccactgg agacggtgct ttcatgaaga
    62701 acatcgaaga tgcaagacgt ctggcaaaga ccatgacgag cattgggaaa ctggctaacc
    62761 gagaaacagt ggcggttatc tcagatatgt cagaacctct tggcgaagct atcggaaata
    62821 gtctagaagt tgtcgaggca atcgaaacat tacaaggaaa tggtccagaa gatctagtag
    62881 aaatgtgcta cgccttagga agccaaatgg ttgttcttgc tggcaaagct aagacaaccg
    62941 atgaagcaag aacgttgctt caagaagctc tggaatcagg taaagcgtta gcaaaattca
    63001 aagaaatgat ccaaaatcaa gggggagacc cagcgatcgt ggaacatcca gaacgaattt
    63061 tgactgcacg ctacacaatg gagcttcctg ctaaacaaag cggagttgtg tcaaaaatcg
    63121 tggcaaatga attaggaatt gctgcaatga tgctaggtgc tgggcgtaaa accaaagaag
    63181 aagatattga tcacgctgtt ggattgaaac tgcataaaaa aattggtgac actgtgacaa
    63241 aaggtgagtc actattgacg atctatagta acgacgaaga aatcacttct gtcattgaat
    63301 tattatataa aaatattgaa attggtgaat cggcaatgaa gccaacgttg attcatgaca
    63361 tcatcacaga ataaaggaaa aaagcggact ttaccgtttt aaagaaaagg ggaaaagaaa
    63421 tggaactaaa ccgaatgatc gaccatacta ttttgaaagc agacgcatct aaagaagctg
    63481 tgatgcaaat tatcgaagaa gcaaaaaaat accattttta ttctgtctgc atcaatccag
    63541 catgggttgc gctagcgaaa gaacaattac aaggtgagcc agtagccgta tgtacagtaa
    63601 tcgggtttcc tctaggagca aacacacccg agacaaaagc atttgaaaca acagatgcca
    63661 tcaataacgg tgcagatgaa gtcgatatgg tcatcaatat cggtgctttg aaatcaaagg
    63721 atgatcaaca agtccaaaaa gatatcgaag cagttgttga agcagcaaaa gatcgtgcgc
    63781 ttgtcaaagt tattgttgaa acatcgttat tagatagaga agaaatcatc cgagcttgcg
    63841 aaatagcaaa agctgcaggc gctgattttg tcaaaacatc aactggtttc tcaacaggcg
    63901 gagcaaaggt agaggatgtc cgtttgatgc gtgaaactgt aggaccagaa atgggtgtca
    63961 aagcttccgg cggtatccat aacgaagaag aagcaatggc aatgatcgaa gctggtgcta
    64021 caagaatcgg aacaagcgca ggagtagcaa tcgtatctgg ttcaactggt gaaggctatt
    64081 aattttttca aaggagaagc tcatggcaga actcaaacaa gaatggatca acacagctat
    64141 tgaagcgtta gataaagcct atgttcctta ttcccatttt ccggtaggcg cttgtctcgt
    64201 aacggaatca ggcaagattt atcaaggcat caacattgag aatgcatcat ttggattaac
    64261 gaattgtgcc gagcgcacag cttttttcaa agcagtatct gaaggagaaa aaacatttac
    64321 gcatctagtc gtagcggggc acacccccga tcctatctct ccttgtggtg catgtcgaca
    64381 agtaatggca gaattctgtt cccctgatat gccggttacg ctagtaggag acaatggtgt
    64441 gacgaaggca acgaccgttc gtgcactgct gccttatgca tttactgaaa aagatctata
    64501 actcatgttc attcagtcag ctgactgttg aaaaataaaa caattatttt aggaggcttt
    64561 acagaaatga aaaaagcaaa attatttggt ttaggtgctg tcgcactagc agcagggtta
    64621 tttttaggtg catgcggcaa caacggctct actgattcat ccggtggcaa aagctcatca
    64681 gacacaacaa ctgcagcatt gatcacagac acaggtggtg tagacgaccg ttcattcaac
    64741 cagtcagctt gggaaggact agaaaaatgg gggaaagatc aaggactttc acgaggaaat
    64801 gatggcttcc aatatttcca atcttcaaat gaatcagatt acatcccaaa tattgaccaa
    64861 gcattgaatg ctggattcaa aacgattttt ggtattggtt ataaactaaa accagcgatc
    64921 caagaacaag caacaaacaa tacaggtacg aactttgtta ttatcgatga tgtcatcgat
    64981 ggattagata acgttgtatc tgcaactttc aaagacaacg aagcttctta tcttgcaggt
    65041 gttgcagcag cttatacgac tgaaacaaac gtagtcggct ttatcggcgg tgtaaaaggt
    65101 gaagtaatcg atcgttttga tgctggattc aaagccggtg ttgatgcagg tgcgaaagaa
    65161 ttaggaaaag aaatcaaagt attgaatcaa tatgctggtg acttctctgc tccagataaa
    65221 ggacgttcaa ttgctcaagg tatgtatgca caaaacgcag atatcatctt ccatgcttca
    65281 ggcggtacag gtaacggtgt attccaagaa gcaaaatcat tgaacgaatc aggcgacaaa
    65341 aaagtttggg ttatcggtgt tgaccgcgac caatcagacg aaggcgaata cacattaaac
    65401 ggcgaaaaga aaaacttcac attgacatct acactgaaag cagttggaac agtagtagaa
    65461 gatttggcac aaaaatcagc ggacggtaaa ttccctggcg gcgaacacac tgtttatgga
    65521 ttgaaagaag acggtgttgg tctaacagaa ggacaacttt ctgacgaagc gaaaaaagca
    65581 gttgatgaag caaaagaaaa aatcatttca ggtgacgtaa aagttccaga aactcctgaa
    65641 gaaaactaaa accatttcta tagccttgtt agacacatgt ttgacaaggc tatggtttga
    65701 ttagaacaag aattgttcat ttatccaagc tgccttgtga cagtttggat aagggaacaa
    65761 ctccctgaag taatgtttgt ttcaaaagaa gaacgctcac taagcgtgaa tcttagtcaa
    65821 tcgttgccct agatagaaat cgttaggatg tgaaaaaaag gtggttcaag aaaatattgt
    65881 catagaaatg cagaatatta ccaaagaatt tggaacattc aaggcaaatg acaacatcaa
    65941 tttacaagta aaggcaggag aaattcatgc gttgcttgga gaaaacggtg ctggcaaatc
    66001 tacattgatg aacgtgcttt ccggattatt agagccgaca tcagggaaaa ttttgatgcg
    66061 tgggaaagaa gtaaagatca caagtccgac caaagccaac caattaggga ttgggatggt
    66121 ccatcagcac tttatgcttg ttgatgcctt tactgtaaca gaaaacatcg tgttgggaag
    66181 cgagcctagt cgcgcaggga tgcttgacca taaaaaagca cgaaaagaga tccaaaaagt
    66241 ttctgaacag tatggattgt cagtcaaccc agatgcttac gttcgtgata tttcagttgg
    66301 aatggaacaa cgggtagaaa ttttaaaaac actttaccga ggagcagatg tactgatttt
    66361 tgatgagccg acagctgtat tgacccctca agaaattgat gaattaatcg tgatcatgaa
    66421 ggaattagtc aaagaaggca agtcgatcat tttgattacg cataagttag atgaaatcaa
    66481 agcagtagct gaccgctgta cagttatccg ccgtggaaaa ggaatcggta cagtcaacgt
    66541 taaagacgta acctcacaac aattagccga tatgatggtt ggaagaacgg tttcattcaa
    66601 aacgatgaaa aaagaagcga agcctcaaga agtcgttttg tctattgaaa atctagtggt
    66661 gaaagaaaat cgtggattag aagccgtgaa aaacttgaac ttagaggttc gtgctggcga
    66721 agtacttggt atcgctggaa tcgatggaaa cgggcagtcg gagttgatcc aagctttgac
    66781 tggtttgcga aaggcagaaa gcggacatat caagctaaaa ggggaagaca tcactaataa
    66841 aaaaccccga aaaatcactg aacatggtgt aggacacgtg ccagaagacc gtcataaata
    66901 cgggttagtc ctagatatga cattatctga aaatatcgca ctacaaacct atcatcaaaa
    66961 accttacagt aaaaacggca tgctgaatta ttcagtaatg aatgaacatg ccagagaatt
    67021 gatcgaagaa tatgatgttc gaacaacgaa tgaacttgtt cccgcaaaag ctttatcagg
    67081 cggaaatcag caaaaagcaa ttatcgctcg tgaagtcgac cgagatcctg atctgttgat
    67141 tgttgcaaat ccaactcgtg gactggatgt aggtgcgatc gaatttattc ataaacgtct
    67201 gatcgaacaa agggacaaat acaaagcagt gttactgatc agtttcgaat tagaagaaat
    67261 tttaaatgtt tcggatcgta ttgctgttat ccatgaagga gaaattgtcg gtatcgttga
    67321 tccgaaagaa acatctgaaa atgaattagg tctattgatg gcgggctata ccttagaaga
    67381 agcacgaaaa gagttagaaa agggggctgg cgcacatgaa tgatcgcagc gaaaaattcc
    67441 gtaatttatt agtgcctgta ctttcagttg ttttaggatt gatcttaggc gcgatattga
    67501 tggctgcttt tggttttcag cccatccaag gttattcttc tatgctagat gcagcccttg
    67561 gttcgcaaag agggataggt gaaacgttaa ggcaagcaac gccgttgatt tttacagcat
    67621 taggtttttc tgtagctaac tcagctgggt ttttcaatat tgggctttct ggacaagcac
    67681 tttgcgggtg gatcgtcagt gtctgggtgg cacttgcgtt tccagatctg ccaaaagttg
    67741 ttttgcttcc gttatgcgtg atcttaggtg ccttagctgg tgcgatggca gcggctgttc
    67801 caggtttact tcgagcattt tttggaacaa gtgaagtgat cgtaacaatc atgatgaact
    67861 atatcttatt atacgtaagc acatttgctt tacatgaagt gatgcctgaa agctatcgct
    67921 caagtttgga ctcttccaat atgatcagtc aaaatgcctc actgcggtta ccatggttaa
    67981 cacaagcttt tggcggctct cgcgtaaact caggattatt tttagcaatc atcgcgctta
    68041 ttttcgtttg gctgctgatg aagaaaacga ctttaggatt tgaaatccgt tctgttggat
    68101 tgaatccttt tgcttctgag tatgcaggaa tgagtagtaa acgtacgatc gttttgtcga
    68161 tggtcatttc tggcgctttg gcagggcttg gcggtgtggt tgaaggatta ggaacgtatc
    68221 aaaacttctt tgttcaaacg acttcgctat ctattggatt tgacggaatg gctgtttcct
    68281 tgcttggttc tggatcagca atcggtattc ttttatcagc gatcttgttc agtattttga
    68341 agatcggtgg acttggcatg cagacaggag caggtgttcc gtttgagatc gtcaatgtat
    68401 cgattgcttt gattatcttc tttgtcggga tcaacttcct gattcgtttc gtcatggcta
    68461 aagtcttcca agggaaaaaa caagaagaga tcgttgcgac aatagaatcg aaaccaacga
    68521 atcaaaacca tgaagggggc gaactataat gtcgactatc gaattaattg ccttgatcct
    68581 tacttctaca ctggtttatt caacaccgct aattctaacg tcactaggcg gaactttttc
    68641 tgaacgaagc gggatcgtaa acgtagggct agagggaatc atggtcatgg gtgcttttag
    68701 ttccatcgtt ttcaacttga cgatgagtcc atcacttggt tctatgacac catggatcgc
    68761 tattttagtg ggtggcgtcg taggtatcct attttctctg attcacgcag tagcaacagt
    68821 cagtttgcgt gctgaccata ttatcagcgg gacagtaatt aatctgatgg cacctgcttt
    68881 aggtgttttc ttgatcaaag catggtatgg taaaggtcaa actgataaca tcacgcagaa
    68941 tctggggtat ttttctttcc caggattagc agatattccg gtcattggac caattttctt
    69001 taaaaataca ttcttacctg cttattgtgc tattttgatt gctgtcctag cttgggtgat
    69061 catcttcaag acgaaattcg gcttgcgcct ccgttcagtt ggtgaaaatc ctcaagctgc
    69121 cgacacatta ggtatcaatg tatatgctat gcgttattca ggcgtactga tttctggctt
    69181 gttaggcggt atgggcggtg ctgtttttgc tcagtcgatt tccggtaact tttcagctgg
    69241 tacgatcgtt ggacaagggt tcatctccat ggctgctatg atctttggta agtggaatcc
    69301 tttaggggca atgggtgcct cattgttctt tggttttgct caaagcttga gtatcattgg
    69361 cgcacaatta ccagttattt cttcgattcc gccagtattg ctgcaaattg ctccatattt
    69421 gctgacaatc attgtgctgg ttgttttctt agggaaagct tctggtccaa aagcaaacgg
    69481 gaagaactat atcaaatcaa aataatcttt tagcgaataa aaagggtggg ttacgggaag
    69541 tgtttttcgt ctcgcccttt ttgttttaaa ggcaataaaa ataaaaagaa agaaggaata
    69601 atcatgttta aacgcgtaca tctgatcgtc atggattcag taggaattgg agaagcacca
    69661 gatgccgata agttcggtga tgttggttct gatacattag gacacattgc gaaagaagca
    69721 ggattgaacg ttccacattt agagcagctt ggtttaggaa cgatccgtcc tcttgaaggt
    69781 gtggaatcag tgaaagatca tcaaggctat gcgacgaaat tagaagaagt ttctgtaggg
    69841 aaagatacga tgacaggcca ctgggaaatc atgggattga atatcaaaac tccatttcgt
    69901 gtgtttccta atggattccc tgatgatctg atcaaacaaa tcgaagaatt ttctggccgt
    69961 aaagtcgtat gtaacaaacc ttatagcgga acagctgtta ttgacgatta cggtgaacac
    70021 caaatgaaaa caggcgattt aattgtatat acttctgccg atcctgtcct tcagatcgcg
    70081 gcacatgaag aaatcattcc tttagaagaa ttatatcgga tttgccaatt cgttcgtgat
    70141 ataacaaaag atgatccgta tatgattggc cgaatcattg cacgtccgta cgtgggtgaa
    70201 ccaggagact tcacacgaac aagcaatcgc catgattatg cgttgaatcc attcggacac
    70261 acagtccttg attctttgaa agaagcagga aaggacgtga ttgctgttgg aaagatcaac
    70321 gatattttca acggacaagg aatcactgaa tctgtccgca cgaaaagcaa tatggatggc
    70381 gttgaccagc tgctgaaagt tatgaaaaac gatttttcag gaatcagctt taccaattta
    70441 gtagattttg atgcattata cggtcatcgt cgggatgtag tcggctatgc gcatgcgatt
    70501 gaagaatttg acttacgaat tccagaattg cttgaagcga tggaagaaga cgacttgcta
    70561 atgattacag ccgatcacgg aaacgatccg acgtttactg gtaccgatca tacgcgagaa
    70621 tatgtaccgt tattggttta cagtaaaaaa atgaaagaac aaggggcatt gcctcaagga
    70681 tattattcag atatttcagc aacgattgct gagaacttta gtgtttcttc gactgaaaat
    70741 ggcgaaagct tcttgaaatt attaaaataa tctttttaaa taggagaaag caaatgacca
    70801 agttaagcga gatgttacaa gaaacgaccc agtttatcct tgataaagga gtaaaagaaa
    70861 ttgattttgg tttgattcta ggttcaggat taggtgaatt agcagatgaa atcgaagatg
    70921 ctgtaatgat ttcttatgat gagatcccgt tctttcctgt ttcgacagta gttgggcatg
    70981 caggaaagct agtttatggg tctctggcag gcaagaaagt gttagccatg caagggagat
    71041 tccactacta tgaaggtcat tccatgcaaa cagtgaccta tccagtacgt gtgatggccg
    71101 gactaggggc ccattcagtc attgtgacta atgcatgtgg tggtgtcaac gaatcatttg
    71161 taccaggaga tttgatgctg attaccgatc atatcaattt tactggtgaa aatcctttga
    71221 tcggtccgaa tgaagatgca atgggcccta gatttccaga tatgagtcaa gcttatacgc
    71281 ctgactatca agcaacagca aaagcagcag cagaaaaact aggtttggat cttaaagaag
    71341 gcgtatatat gggctattct ggaccaactt atgaaacgcc tgcggagatc cgaatgtctc
    71401 ggacaatagg agccgatgca gtagggatgt cgactgttcc agaagtcatc gtagcagctc
    71461 acagcggatt aaaagtgtta ggaatctctt gtattaccaa cttagcagca ggtatgcaag
    71521 cgaatctgag ccatgaagaa gttgtagaaa caactcagag agtcaaacaa tcttttaaag
    71581 cattggttaa agaaacattg acattattgt aataggagga acaataaaat gagtgtgcat
    71641 attgaagcga aagaaggcga gatcgccgat aagattttac ttccgggaga tcctttgcga
    71701 gctaaatata ttgcggagac ttttttggaa gatccagttt gttacaatca ggtacgcggt
    71761 atgttaggct ttaccggcaa atataaagga gaacgtattt ctgttcaagg aacaggaatg
    71821 ggaatgccat cagccaccat ctatgcccat gaattgatcc agtcgtatgg tgtaaagaaa
    71881 ttgattcgag taggtacatg cggtgcattg tcaaaagacg tccatgttcg tgatttagta
    71941 ctagcacaag gtgcagcgac cagttcttcg atgatcgaaa aaaatttcca agcctttcat
    72001 tttcctccaa tcagtgattt caatctatta ctgaaagcat atgagatagc taaagaaaag
    72061 ggctatacgg ttcatgtagg aaatgttctt tcggaagatt ctttctacaa agatgatttg
    72121 acagagacgt tccaattagc tgaacttggc gttttaggag tggaaatgga agcagcggct
    72181 ctttattact taggcgcgaa ataccatgtg cagacattaa gtttgatgac cgttagcgac
    72241 catttgatca ccggtgaaga aacaacagct gcagaacgtc aatctacgtt caatgaaatg
    72301 atcgaagtag gattggaaac agcgatagca gaataagaag gaaatgaaaa agctgccctg
    72361 cattaagtaa taagaacatc caagcaaaaa tagctcctca tgtttttgac ttggctggac
    72421 ttattattgc aaatgtaagc ttttgaacac cgtttaccaa gaggttgtga atctgccgtt
    72481 taactctgag tcacaaggaa caatttcaaa aaacagcttt tcatgtttga tgaatattgt
    72541 gacttgtgac cgagaagttg gcaggagaaa accgtttata cgaaaaaagg gactgtgaca
    72601 aatcgtgtca cagtcccttt tttatagtgt tttttatagg tagtattttt tagcaactgt
    72661 taagtcatca tttaattcat aaacaagtgg ttgacctgtt ggaatttcaa gatccatgat
    72721 gtcttcgtca gagatacctt cgatatgttt agctagggcg cgtaatgagt taccatgtgc
    72781 agctactaaa acagttttgt tgtctagtaa tgctggagca atttcatctt gccagaatgg
    72841 caaagcgcgt tctaatgtca ctttcaagtt ttctccacca ggaatatcgc gtttgtctaa
    72901 cattgcgtaa cgacggtcat ttgctgcaga accttcgtca gtttcttcca ttaatggagg
    72961 aagagtatcg taagaacgac gccagatgtg cacttgttcg tcgccgtatt tttcagctgt
    73021 ttcttttttg ttcaaacctt gtaatttacc atagtgacgt tcgtttaagc gccatgattt
    73081 gatttgtggt acccaaagtt gatctgaata ttctaagatc aagttacatg ttttgattgc
    73141 acgagttaaa acagaagtgt aagcaacatc aaattcgatt ccagcttctt tgattttgcg
    73201 tccgccttct tttgcttctt cgattccttc tggtgctaga tttacatcag cccaaccagt
    73261 aaattggttc aatgcattcc attcactaag accgtgacga gaaaaaacta attttggcat
    73321 gtttagctct ccttttaaga taggtttata tttctgaaat tttcagttct gttttattgt
    73381 acaatttttt ggaagagatt tccatcgaaa aattagataa gattctatga gaaaacaagt
    73441 agagaatata aaaaaacgaa cagtgcccac gcaactgttc gtttttaggt attagatgca
    73501 taattgctcg atttcttttt caggtaaatt gatatattta taacatgttc caactgaaac
    73561 accgcatttt gaagaaataa attggatcgt ttcttttttt tcataataga gacgtcggat
    73621 ttttttgatc gtttttgcat cgatttttgg tcttccgccg atttttcctt ttttacgagc
    73681 ttcatctaat ccaactaatg tacgttcttt gatcaatgcg cactccatgt ttgctaggtt
    73741 ttccattaat tggaaataga tttttcccat ttcgcgcgta tcgattcctt catcaagact
    73801 aatcaagtgc agcccgcgat ctttgaataa ttgcgtgagt tctgttaatt gccgcgtaga
    73861 tttgcctaat cgatgaagtt ggcaaatgac tagtgcatcg ccttgttgca tgtcagcgat
    73921 cactgactcc aaatgaacag cagtagaatc attttgttcc acttcatatg tctcatggaa
    73981 aatttcttga caaccaaagt cagataattg atcaacctga gttgttaaat caacgtcatt
    74041 tatggttgtg cgtgcataac caataatttt catggtgatt cctttctagc agtgattttc
    74101 taactggata taacagtaaa aatgtagcat aaagtacgtc aaaaagatag ttttttcgca
    74161 aattttccgc tgtctttttt tattggatga tcaagcagtt tttctttatt tacgacggat
    74221 tgcgtcaaga atctcaacgc ctttttctga gccgacaact actttagaaa ccatgtttag
    74281 gaaaagccca tgttctacta ccccaacaag ttcatctaaa taggtagcta agctttctgg
    74341 ctgttcgatg acttctagat gcagatcaat gatgtaatga ccaccatctg tggttaagac
    74401 ttctccgttt tcgtctgtac gcaagacggg ttggaatctt ttttcttcaa aaatatgaag
    74461 tagctgttgg gagccataag ggatcacttc aactgggagt gggaacttcc caagtttatc
    74521 gactaattta gaagaatcaa cgatccaaat cgtttctttt gagtacgtag cgactatttt
    74581 ctcgaaaagc aaagcagcgc cgccgccttt gatcccttga aaatcagcac tgatttcatc
    74641 agcaccgtct attgtcaaat cgactacggg gacttcatca atactcttta acggtattcc
    74701 taatttttcc gcttgttctt ttgtcgcatt tgaagtagta actcctgtga tacgtaaacc
    74761 ttcttcacgc atacggcgac caatctcttc taccatataa taggcggttg aaccggtccc
    74821 gagacctacg atcatcccgt cctttacgaa tttcgcggct tcaatgccag ctaattgttt
    74881 taaattcatc tttgcgtcca ccttttttta gtttgagcat cgtgctttcc tcactatttt
    74941 agcgtgattt acggtaaaaa gataccattc ttttttattc caataaagct ttttcaaaaa
    75001 aaattgcatc tcttgttgac atgttttttc attcgtgata ttctaacaaa ggtgctttat
    75061 ctacaggttt ctgtgaagaa acgtgctgac tgtttatcaa agcatatttg ataaggatgc
    75121 agacattgca tttatttttt atcacaaaaa aagaaccacc tggatgtgtg gaactagacg
    75181 caaatcgtgg gaaggaggaa acaaggaatg cctacaatta accaattagt acgtaaacct
    75241 cgtaaatcaa aggtggaaaa atctaattca cctgctttga acaaaggata taacagcttt
    75301 aagaaaactc aaactaacgt aaactcacct caaaaacgtg gggtagcaac tcgtgtgggt
    75361 acaatgacac ctaaaaaacc gaactcagct ttacgtaaat atgcacgtgt tcgtttgtca
    75421 aacttaattg aagttactgc ttatatccca gggattggtc ataacttaca agaacacagt
    75481 gttgtattat tacgcggtgg tcgtgtaaaa gacttaccag gggtacgtta ccatatcgtt
    75541 cgcggtgctt tagatactgc tggtgttaac gaccgtaaac aaagccgttc taaatacgga
    75601 actaaaaaac ctaaagctta attttaatta ttaaacaaat gaaacttgct tctactatat
    75661 agaagtaatt actatgaaca atatttcgga aggaggagtt acggatgcca cgtaaaggtc
    75721 ctgttgcaaa acgtgatgtt ttaccagatc ctatttataa ctcaaaatta gtaactcgct
    75781 tgatcaaccg tgtaatggtt gacggaaaac gcgggattgc agctaacatt atctataatg
    75841 cctttgactt gatcaaagaa tctacaggta acgatccatt agaagtgttt gaacaagcaa
    75901 tgaaaaacgt tcaacctgtt cttgaagtta aagcacgtcg tgttgggggt tcaaactacc
    75961 aagtaccagt tgaagttcgc cctgaacgcc gcgttactct aggtcttcgt tgggtagtaa
    76021 actacgctcg cttgcgcggt gaacacacaa tggaagaacg cttagcaaaa gaaatcatgg
    76081 atgctgcaaa caacacaggt gcttctgtta aaaaacgtga agacactcat aaaatggctg
    76141 atgccaaccg tgcgtttgct cactatcgct ggtaagattc tctctgtttg gtcggctttt
    76201 ataagtcgac ctcagatagt gtccttattt ttagcaacta gatagtaaag agaggagaaa
    76261 caaggagaat gaccagagaa ttttctttac aaaacactcg taatatcggt atcatggccc
    76321 acatcgatgc tggtaaaacg actgctactg aacgtatctt gtactatact ggtaaaatcc
    76381 ataaaattgg tgaaactcat gaaggagctt cacaaatgga ctggatggaa caagaacaag
    76441 aacgtggtat tacgatcact tctgctgcta ctacagcaca atggaagggt caccgaatca
    76501 acatcatcga cacaccagga cacgtagact tcacagttga agttgaacgt tcattgcgtg
    76561 tacttgatgg tgctgtaact gttcttgatg cacaatcagg tgttgaacct caaactgaaa
    76621 cagtttggcg tcaagcaaca acttatggtg tacctcgtat cgtatttgca aacaaaatgg
    76681 acaagatcgg tgctgacttc ttatattcag tatcaacttt acatgaccgt ttgcaagcaa
    76741 acgctcaccc aatccaatta ccaattggtg cggaagacga cttcacagga attatcgacc
    76801 ttgtaacaat gaaggctgaa atgtatacaa acgatcttgg aacagagatc gaagaaacag
    76861 aaattcctga agaataccgc gaattagctg aagaatggcg cgaaaaattg gttgaagctg
    76921 tagctgaaac tgatgaagaa cttactttga aatacttaga aggtgaagaa atcactgaag
    76981 ctgagttaaa agaaggtatc cgccgcgcaa cagttaacgt agaattctac ccagtactat
    77041 gtggttcagc atttaagaac aaaggggttc aattactatt ggacgctgtt cttgattact
    77101 tgccatcacc gttagatatc ccagctatca aagggattga ccctaaaact gatgaagaag
    77161 tagaacgtcc tgcagacgac agcgctcctt tctcagcttt agcatttaaa gttatgactg
    77221 acccattcgt aggtcgttta actttcttcc gtgtgtactc tggtgtttta caatcaggtt
    77281 catacgtaca aaacgcaaca aaaggcaaac gtgaacgtgt tggtcgtatc ctacagatgc
    77341 atgccaactc tcgttcagaa atctctgaag tttacgctgg agatatcgct gctgctgttg
    77401 gtttgaaaga cacaactact ggggacacac tatgtgatga aaagagtcta gttattcttg
    77461 aatccatgga attcccagaa ccagttatcc aagtcgctat tgaacctaaa tcaaaagctg
    77521 accaagataa aatgggtgtg gctttacaaa aactatctga agaagatcct actttccgtg
    77581 ctgaaacaaa cgtggaaaca ggcgaaacaa tcatcgctgg tatgggtgaa cttcacttag
    77641 atatcatcgt tgaccgtatg cgtcgtgaat tcaaagttga agctaacgta ggtgcgcctc
    77701 aagtatctta ccgtgaaact ttccgtgctg gcactcaagc cgaaggtaag tttgtacgtc
    77761 agtctggtgg taaaggtcaa tacggtcacg tatggattga atttacacca aatgaagaag
    77821 gtgctggttt cgaatttgaa aatgcgatcg tcggtggggt tgttcctcgt gaatacattc
    77881 cagctgttga aactggtttg aaagatgcta tggaaaatgg tgtcttagct ggctatccat
    77941 tagtagatat caaagctaaa ctttacgatg gttcatacca tgatgtcgat tcaaatgaaa
    78001 ctgccttccg tgttgctgct tctatggcat tacgtgcagc agctaagaaa gctaatccag
    78061 taatccttga accaatcatg gctgtagaag ttgttatccc tgaagattat ttaggtgatg
    78121 ttatgggtca cgtaactgct cgtcgtggac gtgttgaagg tatggaagct cgcgctggcg
    78181 gccaacaagt tgttcgtgcg atggttccac ttgctgaaat gtttggttat gctacaactc
    78241 ttcgttctgc tactcaaggt cgtggtacat ttacaatgac atttgaccac tacgaagatg
    78301 taccaaaatc agtacaagaa gaaatcatta agaaaaacgg cggaaaagct gaataatttc
    78361 ttaaaaccgg atagtcctgc aaagagatta tccaaagaga ctatattttt aatatagttt
    78421 cgtgtaaaat aattatcgat gatatgggca tgtggtgtac cttgtgtaca tcatatacct
    78481 atcagacaaa taaaaaatat attgtatttt taggaggaca ttttaaaatg gcaaaagaaa
    78541 aatttgaccg ttctaaacca cacgttaaca ttggtactat cggacacgtt gaccatggta
    78601 aaactacatt aacagctgca atcacaactg tactatctaa gaaaaacggc ggccaagcta
    78661 tggcttacga tcaaatcgat ggtgctccag aagaacgcga acgcggaatc acaatctcaa
    78721 cagctcacgt tgagtatgaa acagatacac gccactacgc tcacgttgac tgcccaggac
    78781 acgcggatta cgttaaaaac atgatcactg gtgctgctca aatggacgga gctatcttgg
    78841 tagtttctgc tgctgacggc ccaatgcctc aaactcgtga acacatccta ttgtctcgtc
    78901 aagttggtgt tccttacatc gttgtattct tgaacaaagt agacatggtt gatgacgaag
    78961 aattactaga attagttgaa atggaagttc gtgacctatt aacagaatac gaattccctg
    79021 gtgacgatgt tcctgtagtt gctggatcag ctttgaaagc tctagaaggc gacgcttcat
    79081 acgaagaaaa aattcttgaa ttaatggctg cagttgacga atacatccca actccagaac
    79141 gtgacaacga caaaccattc atgatgccag ttgaagacgt gttctcaatt actggacgtg
    79201 gtactgttgc tacaggtcgt gttgaacgtg gacaagttcg cgttggtgac gaagttgaag
    79261 ttgttggtat tgctgaagaa acttcaaaaa caacagttac tggtgttgaa atgttccgta
    79321 aattgttaga ctacgctgaa gctggagaca acattggtgc tttactacgt ggtgttgcac
    79381 gtgaagacat ccaacgtgga caagttttag ctaaaccagg tacaatcaca cctcatacaa
    79441 aattctctgc agaagtatac gtgttgacaa aagaagaagg tggacgtcat actccattct
    79501 tcactaacta ccgtccacaa ttctacttcc gtacaactga cgtaacaggt gttgttgaat
    79561 taccagaagg aactgaaatg gttatgcctg gcgacaacgt aacaatggaa gttgaattaa
    79621 tccacccaat cgctatcgaa aacggtacta aattctcaat ccgtgaaggc ggacgtacag
    79681 ttggtgccgg tgtcgttaca gaaatcaaag cttaattcac ttaagtttta aagtaaagca
    79741 acatttattc ggacgtaatc gctcggaacg aaggtcttag gatctttgtt ccgagctttt
    79801 ttatttattg caggatcagg aaaaaagaca ggagtattat aaatagaaga aacacgaaat
    79861 aaaagagtca gtattttcta aatgaaactt tatgaaaaca aaatgttttt tacaagaaaa
    79921 agccttgctt tgtgagttta tatatattat aatgacaaag gtgcgtttac tttagtgaat
    79981 tgcacagacg taatcagaat gatttcgtcg gctaagaaac gagaggttgc gacacgcccg
    80041 gacgctttgc cacgaacgag cgtgacggaa attttcgtgg agctatgtct acttttcaaa
    80101 ataggcgaag gagggaacaa gatggcaaaa caaaaaattc gtatccgttt aaaagcgtat
    80161 gaacaccgta ttttagatca atcagcggat aaaatcgtgg aaactgcaaa aagaactgga
    80221 gctagcgtgt ctggtccaat tccattacca actgaacgca gtctttacac aatcatccgt
    80281 gcgactcata aatacaaaga ttcacgcgaa caattcgaaa tgcgtacaca caaacgtctt
    80341 atcgacattg tgaacccaac accaaaaact gttgatgctt tgatgaagct agacttacca
    80401 tctggtgtaa acatcgaaat caaattataa aactaaaaca aaaataatgg aggtgtactc
    80461 atgaccaaag gaatcttagg gaaaaaagtg ggaatgacac aaatcttcac tgaatctggt
    80521 gaattaattc cagtaactgt tgtagaagct actccaaacg tagtactaca agtgaaaaca
    80581 atggaaacgg atggctacga agctattcaa gttggttacc aagatatgcg tgaagtttta
    80641 tcaaacaaac ctgcgaaagg tcatgttgca aaagcaaaca cggctcctaa gcgcttcatt
    80701 cgtgaattca aaaatgttga gctaggagaa tatgaagtag gaaaagaaat tacagtagat
    80761 gttttccaag caggagacat tattgatgta acaggtacta cgaaaggtaa aggattccaa
    80821 ggggttatca aacgccacgg acaaagccgc ggaccaatgg ctcacggttc tcgttaccat
    80881 cgtcgtcctg ggtcaatggg tccagttgca cctaaccgtg tatttaaaaa caaacgtctt
    80941 gctggccgta tgggtggcaa ccgcgtaact atccaaaact tggaagttgt acgtgtcgat
    81001 gcagaaagaa atgttatctt gatcaaaggt aatgttcctg gagctaaaaa atctttaatt
    81061 actatcaaat cagctgtgaa agctaaataa ttgtaggaag aaaggaggaa ctaaggaatg
    81121 ccgaatgtag cattattcaa acaagatgga agccaaaacg gtgaaatcac acttaacgaa
    81181 gaaatcttcg gaattgaacc taatgaaagt gttgtttacg atgcaatcgt aatgcaacgt
    81241 gcgtcattaa gacaaggaac acacgcggtt aaaaaccgta gcgctgttcg cggcggcgga
    81301 cgtaaaccat ggcgtcaaaa aggaacaggt cgtgcacgtc aaggatctat ccgctcacca
    81361 caatggcgtg gaggtggcgt agtctttgga ccaacaccac gttcttacag ctacaaactt
    81421 cctaaaaaag ttcgtcgttt agcaatgaaa tctgtattgt cagaaaaagt tgctgaaaac
    81481 aacttggtag caatcgaagg cttgaacttc gacgcaccaa aaacaaaaga attcaaacaa
    81541 gttcttgcta acttatctat cgactctaaa gtattggttg ttctagaaaa aggtaacgac
    81601 tttgctgctt tatcagcacg taacttacca aacgtttctg tagtaacttc tgataatgtg
    81661 agtgtgctag acgttgtatc aaatacaaaa gtgttagcaa cacaaactgc tcttactcaa
    81721 attgaggagg tgcttgcata atgaacttac tagacgtaat caaacgccca gtgatcactg
    81781 aaaaatcaat gcttgccatg gatgacaaga aatacacttt tgaagtggac actcgcgcaa
    81841 acaaaacttt agtaaaacaa gcggtagaag ctgcttttga cgttaaagtc aaaaacgtga
    81901 acatcgtgaa cgtacgtgcg aaattcaaac gtatgggcaa atatgcagga tatacaaaaa
    81961 aacgtcgcaa agcgatcgtt acattgactg aagattcaaa agaaatccaa ttattcgaag
    82021 ctgctgaata agcactaata gataactcat taaattagga gggaaaaacg tggcgattaa
    82081 aaagtacaaa cctacctcaa atggtcgtcg taacatgaca ggttctgatt ttgctgaaat
    82141 cacaacatca acacctgaaa aatcattgtt acaaccattg aaaaaccatg ctggacgtaa
    82201 caacaacggc cgcatcactg tacgtcatca aggtggcgga cacaaacgcc aatatcgcgt
    82261 gatcgacttc aaacgtaaca aagataacgt tgttgcaact gttcaaacga tcgaatatga
    82321 tccaaaccgt tcagctaaca tcgcgttagt acactatgaa gatggagtga aagcttacat
    82381 cttagcacct aaaggactag aagtgggtat gaaacttgtt tcaggtccag aagcagatat
    82441 caaagtcgga aatgctcttc cattagaaaa cattccagtt ggtactgtta tccacaacat
    82501 tgaaatgaaa cctggtaaag gtggacaatt gatccgttct gctggtacaa gtgctcaagt
    82561 acttggtaaa gaaggcaaat acgtattgat ccgcttgaac tctggtgaag ttcgtatgat
    82621 cttagctgca tgccgtgcta caatcggttc tgtaggtaac gaacaacacg aattaatcaa
    82681 catcggtaaa gctggacgct ctcgttggat gcgtaaacgc ccaactgtac gtggtagcgt
    82741 aatgaaccct aatgatcacc cacacggtgg tggtgaaggt aaagctccta tcggacgtaa
    82801 agcaccagtt tctccttggg gtcaaccagc tcttggttac aaaactcgta acaaaaaagc
    82861 taaatcagac aaacttattg ttcgtcgtcg caaaaataaa taattacttt gaccatgtgt
    82921 cgcacatttt gagaggaggt tcaccatggg tcgtagttta aagaaaggac cttttgtcga
    82981 tgagcattta atgaaaaaag ttgaagctca agcaggggtt gaaaagaaaa aagttatcaa
    83041 aacttggtca cgccgttcaa cagttttccc aagttttgtt ggatatacca tcgcagttta
    83101 tgatggacgt aaacatgtac cagtttacat ccaagaagac atggtaggac acaaattagg
    83161 tgaatttgca cctacaagaa cttatcgtgg acatgctgct gacgataaga aaacgaaacg
    83221 ctaatttgag gggaggataa atccaaatgg cagaacaaat cacatcagct aaagcaactg
    83281 caaaaacagt tcgcacttca cctcgaaaag cccgtttagt aattgacctt atcagaggta
    83341 aaagtgtcgc agatgcaatt tcaatcttga aattcacacc aaacaaatct gctggaatca
    83401 ttgaaaaagt attgatgtca gcagttgcga atgcagaaaa taactttgac ttagatgttg
    83461 aaaacttggt agtaagcgaa gcttttgtta acgaaggacc tacaatgaaa cgtttccgtc
    83521 ctcgtgcaaa aggatcagct tctccaatca acaaacgtac aagtcatatc acggtagtcg
    83581 tatcagaaaa ataaggaggg ataatcagtg ggtcaaaaag tacatccaat tggaatgcgt
    83641 gtaggcatca tccgcgactg ggatgctaaa tggtatgctg aaaaagagta tgctgagttc
    83701 ttacacgaag atttaagaat ccgtaaattt atttcaacta aacttgctga tgctgctgta
    83761 tcaacagttg aaatcgaacg cgcaacaggt cgcgtaaaca tttcaattca cacagctaaa
    83821 ccaggtatgg ttattggtaa aggcggttct gaagtcgaaa gcctaagaaa agaattaaac
    83881 aaattaactg gtaaaagaat ccatatcaat atcgtggaaa tcaaaaaacc agatctagat
    83941 gcaaaattag taggtgaagg aatcgcacgt caattagaaa accgtgttgc tttccgtcgc
    84001 gctcaaaaac aagccatcca acgctcaatg cgtgctggcg ctcaagggat caaaactcaa
    84061 gtatctggtc gtctaaacgg agcagatatc gctcgttcag aaggatactc agaaggaact
    84121 gttcctcttc atactttacg tgcggacatt gattacgcat gggaagaagc agatacaaca
    84181 tacggaaaac taggagttaa agtgtggatc tatcgtggag aaattcttcc aactaaaaaa
    84241 aacactgaga aaggagggaa ataatcatgt tagtacctaa acgtgtaaaa caccgtcgtg
    84301 aattccgcgg aaaaatgcgc ggggaagcta aaggcggaaa agaagtagca ttcggtgaat
    84361 acggtttgca agctgttgat tcacattgga tcacaaaccg ccaaatcgaa gctgctcgta
    84421 tcgcaatgac tcgttacatg aaacgtggtg ggaaagtatg gattaaaatt ttccctcaca
    84481 aatcttatac tgccaaagca attggggtac gtatgggttc tggtaaaggg gcacctgaag
    84541 gatgggttgc accagtaaaa cgtggtaaaa tcatgtttga aatcgcaggc gttcctgaag
    84601 aagtagctcg tgaagcgtta cgtctagctt ctcacaaatt accaatgaaa actaagatcg
    84661 taaaacgtga ggaaatgggt ggtgaatcga atgaaggtta aagaaatcag agaattaacc
    84721 actgccgaaa tgcttgatca agaaaaacaa ttaaaagaag aattgtttaa tcttagattc
    84781 caattagcaa caggtcaatt agaaaacact gcacgtatta aagaagtacg taaatcgatt
    84841 gcacgcatca aaactgtgtt gcgtgaacaa gctaagtaat tatggaagga ggccattaag
    84901 cgtatgactg aaggaagaaa tcaacgcaaa gtttatcaag gtcgcgtggt ttcagacaaa
    84961 atggataaaa caattaccgt tgctgtcgaa acaaagaaaa accaccctat ctatggcaaa
    85021 cgcatgaact attctaagaa atacaaagtg catgatgaaa acaacacagc aaaagttggc
    85081 gacatcgtga gaattatgga aactcgtcca ttatcagcta caaaacgttt ccgtttgttg
    85141 gaagtagtcg aagaagcagt tattatctaa taacacgaga ttttcctata tagattgaat
    85201 gcaaaatctg aaaggaggat acacaacgtg atccaagcag aaagtcgttt aaaagtcgct
    85261 gataactcag gcgcacgtga aattttgacg atcaaagtcc ttggtggatc tggtcgtaaa
    85321 actgctaaca tcggtgatgt gatcgttgct acggttaaac aagcaacgcc aggtggggtt
    85381 gtcaaaaaag gtgacgtcgt taaagccgtt atcgttcgaa caaaatcagg agctcgtcgt
    85441 acagacggtt catatattaa atttgatgaa aatgctgcag tgattattcg tgatgataaa
    85501 agccctcgtg gaacacgtat ctttgggcca gttgcacgtg aactacgtga aaacaacttc
    85561 atgaagatcg tttcactagc accagaagtt ttataatctt gagacactta tcaaaggagg
    85621 tgcgaaacag ttatgtttgt taaaaaaggc gataaagtta aagttatcac tggaaaagac
    85681 aaaaacaaag aaggcgttgt actagcagcg ttcccgaaaa aagataaagt tattgtcgaa
    85741 ggtgtaaatg tcgttaaaaa acaccaaaaa ccttctcaag ctgctcctca aggtggaatt
    85801 gttgagatgg aagcgccaat tcatgtttct aacgtgatgg tggttgactc tactggtgta
    85861 gctggccgag ttggctacaa agaagtagac ggcaaaaaag tccgtgtttc taaaaaaacc
    85921 ggtgaagttt tagataaata aaagtttaag gaaggagggt cttactgaat gaaccgccta
    85981 aaagaaaaat atcttaaaga agtaactcca tcattgatgg aaaaatttaa ctatagctct
    86041 gttatgcaaa cacctaaagt tgaaaaaatc gtcatcaaca tgggtgttgg tgatgcggta
    86101 tcaaacgcta aaaacttaga taaagcagtt gaagaattaa ctttgatcac tggtcaaaaa
    86161 ccaatgatta caaaagctaa aaaatcaatc gctggattcc gtttacgtga aggaatgcca
    86221 attggtgcaa aagttacatt gcgcggagaa agaatgtacg aattcttaga caaattggtt
    86281 tctgtttcac taccacgtgt acgtgacttc catggtgtaa gtaaaaaagc ctttgacggt
    86341 cgtggaaact atactttagg tattaaagaa caattgatct tcccagaagt agattatgat
    86401 ttagtagata aagtacgtgg tatggacatc gttattgtaa caacagcaaa ctctgatgaa
    86461 gaatctcgtg agttgttgac acaattaggc atgccattcc aaaaataatt aaaggaggcg
    86521 aactacgtgg ctaaaaaatc aatgattgct aaaaacaaac gtcctgcaaa attttcaaca
    86581 caagcttata ctcgttgcga acgttgcgga cgtccacatt cagtttatcg taagttccat
    86641 ctttgccgta tttgcttccg cgaacttgcc tataaaggtc aaattcccgg cgtgaagaaa
    86701 gctagctggt aaaaagtaaa ttcgcataag aaggaggtaa aagatcaatg gtcatgacag
    86761 atccaattgc agattttcta actcgtatcc gtaacgcaaa catggttaaa catgagtctt
    86821 tagaagtacc tgcatcaaaa attaaacgtg acatcgctga aatcttgaaa cgtgaaggtt
    86881 tcgttcgtga tgttgaatac atcgaagatg acaaacaagg cgtgatccgt gttttcctaa
    86941 aatttggtaa aaacggcgaa cgtgttatca caaacttaaa acgtatctct aaaccaggtt
    87001 tgcgtgctta tgtcaaagct gacgaagtac caaaagtatt gaacggttta ggtatcgcaa
    87061 tcatttcgac ttctgaaggt gttgtcactg ataaagaagc tcgtgaaaaa aatatcggcg
    87121 gcgaagttat cgcttacgta tggtaatcaa gtagaatcta caaggaggtg tctttaagtg
    87181 agccgtatcg gaaataaagt cgttgttatc cctgaaggag taacagttac tcaagacgga
    87241 aacaacgtta cagttaaagg accaaaaggt gaattgactc gtactttttc agcagatatc
    87301 aaaatgaata tcgaaggaaa cgaagtaact ttcactcgtc caaacgacag taaagaaatg
    87361 aaaacaattc atggaacaac tcgtgcgaac ttcaacaaca tggttgtagg tgtcagcgaa
    87421 ggtttccaaa aagcgttaga actaatcggg gttgggtacc gtgcacaatt acaaggatca
    87481 aaacttgtat tgaacgttgg ttactcacat ccagtagaaa tcacaccacc agctggagta
    87541 actgttgaag taccagctaa cacacaagta attgttaaag gtgcgaacaa agaagaagtt
    87601 ggcgaattag ctgcaaacat tcgtggaact cgtcctccag aaccttataa aggtaaaggt
    87661 attcgttatg ttggcgaata tgtacgccgt aaagaaggta aaactggtaa ataatcgttt
    87721 agaaaaattg catagaagcc agcagcagga aaaaggcatt tggctgaagt ttaccttcaa
    87781 aaaaatacat ttttacttgt gccgcagctt ctatttttcg aaacgtattt actgcttgaa
    87841 atagtagtac aataaaaata aagaggtgac aattgtgatt acaaaaccag ataagaacaa
    87901 aacacgtcaa aagagacatc gtcgtgtacg taacaatatc tctggtactg ctgagcgccc
    87961 acgcttgaac gtttttcgtt ctaacaaaaa catctacgct caaattattg atgacgtagc
    88021 gggtgtaacg ctagcaagtg cctctacctt ggataaagaa atttcaggtg gaacaaaaac
    88081 agaagcagca caagctgtcg gtaaactagt tgctgaacgt gcagctgaaa aaggcattaa
    88141 agtagtagtc tttgaccgtg gtggatacct ttaccatggc cgtgtgcaag ctttagctga
    88201 agctgctcgc gaaaatggac tagaatttta gaagaaggag gaataccatt catggtttat
    88261 atcgatccaa aacatttgga attagaagac cgcgttgttg caattaaccg tgtaacaaaa
    88321 gttgttaaag gtggacgtcg tctacgtttt gctgctttag ttgttgtcgg tgacaaaaac
    88381 ggacacgtag ggtttggtac aggtaaagct caagaagttc ctgaagcaat ccgtaaagca
    88441 attgaagatg cgaagaaaaa cttagttgaa gtacctatgg ttggttcaac aatcccacac
    88501 gaagtaatcg gtgcttttag tggtggtaga atcctaatga aacctgctgt agaaggttct
    88561 ggggttgctg ctggtggacc agttcgtgcc gtattagaat tagctggggt agcagatatc
    88621 acttctaaat ctttaggctc aaatacacca atcaacgttg ttcgcgcaac agttgaaggt
    88681 ttgaaacaat tgaaacgtgc agaagaagtt gcagaacttc gcggcaaatc agttgaagaa
    88741 ttaatcggat aaggaggaca acaataatgg ctgaattaaa agttacttta aaacgcagta
    88801 ttatcggacg tcctcaaaac caacgcgata cagttaaagc gttaggtctt acaaagatta
    88861 acagttcagt ggttaagcct tctaatgaag caattaaagg catgatcaat actgtttctc
    88921 acctagtgga cgttgaagaa atttaattta gaaaaatcat aactagaaca ttaaggaggt
    88981 gccaaaccta tgaaacttca tgaattaaaa cctgctgaag gttcacgcca agtacgtaac
    89041 cgtgttggtc gtggtacttc atctggtaac ggaaaaactg ccggacgcgg acaaaaagga
    89101 caaaaagctc gttcaggcgg cggtgtgcgt ttaggatttg aagggggaca aacaccattg
    89161 ttccgtcgtt taccaaaacg aggattcaca aacgtgaacc gtaaagacta tgcagtcatt
    89221 aatcttgatg tattgaaccg ctttgaagac ggagctgaag taactcctgt tgctttagtt
    89281 gaagctggaa tcgtgaaaaa cgaaaaagct ggaatcaaag tattagctaa tggggaacta
    89341 aacaagaaat taactgtgaa agcagcaaaa ttctctaaag cagctcaaga agcaattgaa
    89401 gcggctggtg gatcaatcga ggtgatctaa tgtttagact attaaaagat gcttttaaag
    89461 taaaagacat tagatcgaaa atcctattta ctgtatttgt gttgtttgta tttcgcctag
    89521 gtgcacatat tactgtacca ggcgtaaatg ctgcttcatt gcaaaacctt tcttctttgc
    89581 catttttcaa catgttgaac ttggtaagtg gtagtgcgat gcaaaacttt tccattttct
    89641 caatgggggt ttcaccatac attactgctt ctatcgtcat tcagcttctt caaatggaca
    89701 tcgtgcctcg atttgttgaa tggtcgaaac aaggggaagt tggtcgtaaa aaactgaatc
    89761 aagcgactcg atatctgaca ttagtattgg catttatcca atctgtcggt atcactgctg
    89821 gattcaatca atacgctcaa ttaggtttcg tgaataatcc taacgtagca acttacgtaa
    89881 cgatcggttt gattctaaca gccggtacga tgtttgttac ttggctagga gaacagatca
    89941 cagaaaaagg tatcggaaat ggcgtatcaa tgatcatttt tgcaggtatc atttctagat
    90001 tgcctgaaag tatcagagaa atttatgaag attactttat aaatattgat ccttcagaca
    90061 tatggaaatc tgctattttt gtggccattt tactagtagc ggtcttgttg atcgtgacat
    90121 tcgtaacatt tttccaacaa gctgaacgca agatcccgat ccaatatacg aaacgcgtag
    90181 ctggcgcgcc tacaagcagc tatctgccgt tgaaagtaaa tgtagctgga gttattccag
    90241 ttatctttgc tagttcgttt attgctacgc caaatgctgt tttacaagca ttgagcggta
    90301 gattcggttc tgaaaactgg tacaaagtga tgatggagat ctttaactat aatacagcac
    90361 ctggtgctac gatttatacg attctgatcg ttgccttcac tttcttctac gcctttgttc
    90421 aagtaaaccc tgagaaatta gcggaaaact tgcaaaagca aggaagttat ataccaagcg
    90481 tacgccctgg taaggggact gaagagtatg tctcaagatt actgatgcgt ttaagtacag
    90541 ttggtgcagt cttccttggt ttagtggcat tgcttccaat cattgctcaa atggtttggg
    90601 atcttccaca atcaatcgga ttaggcggta caagcttact gatcgttatc ggtgtagctt
    90661 tagaaactgc gaaacagtta gaaggcttaa tgctgaaacg taaatatgtt ggattcattg
    90721 agtaagaaag cgtgttgagg tttcccctca acacacgttc tcacacattt attaggaggg
    90781 aaaacaatga acctcatttt aatggggctg ccaggcgcag gtaaaggaac acaagctgaa
    90841 aaaatcattg atgcttatgg tattccgcac atctcaacag gagatatgtt ccgcgcagcg
    90901 atgcaaaacg aaacagctct tggcctggaa gcaaagtctt atatggataa aggtgcatta
    90961 gttcctgatg aagtaacaaa tggaatcgtg aaagagcgac tagctgaacc agatacagag
    91021 aaaggcttct tattagatgg ctttccacgt acgctagatc aagcaaaagc tctggacgca
    91081 atgctaaaag atttgaataa aaaaattgat gctgtcatcg atatccatgt tggtgaagaa
    91141 atcttggtcg aacgtctagc cggccgattt atctgcagca attgtggagc aacttaccat
    91201 aaaattttca accctacaaa agttgaagac acatgtgatc gttgcggtgg gcatgaattc
    91261 tatcaaagag aagatgataa acctgagacg gttaaaaatc gtctggctat caacatcaaa
    91321 aatagtgaac caatcttggc ttattataaa gaacaaggtt tgctaaacac tattgatggt
    91381 gctcgtgaaa tcgatgctgt atttgcagat gtacaaaaaa tcattgaaaa atagcacgat
    91441 tgcccgtagc cgacttgcat tatgcagtta ctcttgtcaa gttcaataaa ttatgataga
    91501 atatgtcagt ccgacttcta gagaatcctc tagatgatcc attttgagaa ttttgaggtg
    91561 ggaactgcag tttccgccat tttatcgtgt gaaaatgcga aacaagtaca aggaggtact
    91621 gtgcgtggca aaagaagata tgattgaagt cgaaggtaca gtcgtcgaaa ctttgccgaa
    91681 tgcaatgttt aaagttgaac tagaaaacgg acaccaagtt cttgctactg tttcaggtaa
    91741 aattcgtatg cattacatcc gtatcttacc tggtgacaaa gtaacggttg aattgtctcc
    91801 atacgatctt aaccgtggcc gtattactta tcgctttaaa taattgtact ccgtaaaact
    91861 acaggaggta ttatcatgaa agtaagacca tcagtaaaac caatgtgcga acattgtaaa
    91921 gtaattcgtc gtaaaggacg cgttatggtg atttgcccag caaatccaaa acataaacaa
    91981 cgtcaaggat aattggaggt gctataaata tggctcgtat tgcaggagta gatattcctc
    92041 gtgataaacg tgtagtagtt tcacttactt atatctatgg tattggtaac actactgcta
    92101 aaaagatctt agctgatgct ggtgtatctg aagatgttcg cgttcgtgaa ttaacgaatg
    92161 aacaaacaga tgctatccgt gcagaagtag ataaattaaa agttgaaggg gatcttcgtc
    92221 gtgaagtgaa cttgaacatc aaacgtttga tggaaatcgg ttcttaccgt ggaatccgtc
    92281 atcgccgtgg attgcctgtt cgtggacaaa acacgaaaaa caatgcacgt actcgtaaag
    92341 gcccagctcg cgctatcgct ggcaagaaaa aataattctt aagtgaagga ggttaaaact
    92401 tcatggcagc aaaaaaagtg aatcgtaaac gccgtgtgaa gaagaatata gaagcaggta
    92461 ttgcacatat ccattctaca ttcaataaca ctatcatcat gatcactgat actcacggaa
    92521 atgcattagc gtggtcatca gctggttcat taggtttcaa aggaagcaaa aaatcaactc
    92581 cttttgccgc tcaaatggca gcagaaactg ctacaaaagc agcaatggaa cacggattaa
    92641 agactgttga agtaacagtt aaaggacctg gttctggacg tgaagcagca attcgttcat
    92701 tacaagctac aggtttagaa gtgactgcaa ttcgtgacgt gactccagtt cctcataatg
    92761 gtgcccgccc tccaaaacgc cgtcgtgttt aatgagtgcc gctcattttg agttttatca
    92821 gtaacgtcat tgaacaagac acttttcgtt ttgaaagggg taaagataag aatgattgag
    92881 ttcgaaaaac caagaatcgc aaaaattgat gaagaaaaag attatggcaa gttcatcgtt
    92941 gaacctcttg aaagagggta tgggactact ttagggaatt ccctacgtcg tattttatta
    93001 tcttctttgc caggtgctgc catcactaat attcaaatcg atggtgtact gcatgaattc
    93061 tctaccatca aaggtgttag agaagacgtc actcaaatca tcttgaacat taaaggctta
    93121 gcgctgaaaa tgtatggcca agaagaaaaa acccttgaaa tcgatattac cggacctgct
    93181 acagtaactg ctggcgatat tatcgttgat agtgatgtag aaattcttaa caaagatatg
    93241 tacatctgta gtgtctctga aggagctacg ttccatgctc gcttaacagt gaaacctgga
    93301 cgtggttatg ttcaagcaga tgaaaataaa aaagaagata tgccaattgg cgttcttcca
    93361 gtcgattcaa tctacacacc tgttcgtcgt gtgaactatc aagttgagaa cacacgtgtt
    93421 ggacaccgtg aagattttga taaattgacg atggagattt ggacagatgg ttcaattgaa
    93481 ccgttggaag caatgagttt gtctgcgaaa atcatgactg aacatttgga tatttttgtg
    93541 aacttaactg atgaagcaaa aaatgctgaa atcatggtcg aaaaagaaga aacacaaaaa
    93601 gaaaaaatgt tggaaatgac gatcgaagag ttagacttgt ctgttcgctc ttacaattgt
    93661 ttgaaacgtg caggaatcaa tactgtacaa gaacttacaa ataaatctga gcctgaaatg
    93721 atcaaagtcc gtaacttagg acgtaaatca ttagaagaag ttaaagcaaa attgcacgac
    93781 ttaggtttag gtttacgtaa agaagattaa aaccttttac gaaggaggga aaccaacgtg
    93841 agttatcgta aactaggacg cacatctagc caacgtaaag cgatgttgcg tgatttaaca
    93901 actgatttat taatcaacga acgtattgtg actactgaag ctcgtgcgaa agaagttcgc
    93961 tcaactgcag aaaaaatgat cactttaggt aaacgcggag atttgcatgc acgtcgtcaa
    94021 gctgcggctt tcgtacgtaa tgaagttgcc agcgtgcgtg aagaaaatga ggacatcgtc
    94081 attgaatcag ctttacaaaa actgttcaac gatattgcac ctcgttacgc agaacgtcaa
    94141 ggcggataca cacgcatttt gaagacagaa ccaagacgcg gtgatggtgc accaatggtt
    94201 gtcttagaac ttgtctaaaa tcacactaat aacatcactt tatagatgat tcttttagag
    94261 tgttatgatg ttggaagata tttttccgag tctagctcag cgctgctcct caagcgaata
    94321 tcgcttgggg agtaagcact catcataaag tgttttttta taccttcaat tacctgtcat
    94381 gacagatatg tttgaaggca taaaaaaaca ttttaagcga gaaataattg aaaattttgt
    94441 ctttttttga tagtgttagt aggaacttta ggatgaagaa ggagtaatca tggagccaat
    94501 tattgaatta gaaaagataa attataaata ccagccagat gatcttcgcc ccgcattgaa
    94561 agatgtctcc tttacaattg ataaaggtga gtggatcgct attattggac acaatggttc
    94621 aggaaaatcg acacttgcca aaacgatcaa tggattgctt ctgccagaat cagggatcgt
    94681 gaaagtggga aatcaaatat tggatgaaga aaatatttgg acgatccgac agatggttgg
    94741 aatggttttt caaaatccag acaatcagtt tgttggttcg acagtagaag atgacgttgc
    94801 ttttggtctt gaaaatcaag ggattcccag agaagaaatg ctagtgcgtg tgaaagatgc
    94861 acttgagaaa gttagaatgg ctgagtttgc ttcacgcgag cctgctcgtt tatcaggagg
    94921 gcaaaaacaa cgtgttgcca ttgccggcgt ggttgcctta cgacctgata tcattatttt
    94981 agatgaagca accagtatgc tagatccaga aggtcgtgaa gaagtgatct ctacgatcaa
    95041 gaagatcaaa gaagaaagtc aattgaccgt tatatctatc actcatgata ttgatgaagc
    95101 agctaatgcc aatcggatac tagtgatgag acaaggcgaa cttgtccgtg aaggtacacc
    95161 aaaagaaatt ttttctgctg gtcctgaact gatcgactta ggtctagatc taccatttcc
    95221 tgaaaaactg aagagcgcac tgaaagaacg tggggttgat gtgcccagtg aatatatgac
    95281 agaagaaagg atggtggatt ggttatggac atccgttttg aacaagtaga cttcacttat
    95341 cagccgaata caccatttga acaaagagcc ttatttgaca tcaatatgac gatcaaagaa
    95401 aacagttata ctgcattagt agggcatacc ggaagcggga aatcaacctt acttcagcat
    95461 ttgaatgcgc ttgttaagcc aacaagcggt acagtccata ttggagaacg agatattcag
    95521 ccggatacgg ataataagaa tttaaagccc atccgaaaaa aagttggcat cgtctttcag
    95581 tttccagaag cacagctatt cgaagaaacg gtagcaaaag acattgcttt cggtcctaaa
    95641 aactttggtg tcagcgaaga agaagcacta gtcctagcaa aagaaacatt agaacaagtt
    95701 gggctggatg aaagctattt ggaacgttcg ccatttgaac tttcaggagg gcaaatgcgt
    95761 agagtagcaa tcgctggtgt gcttgccatg aggccggaag tacttgtatt ggatgaacca
    95821 acggcaggac ttgatcccca gggtagaaaa gaaatgatgg agatgttttg gcgtcttcat
    95881 aaggaacagc agatcactat tgtattagtt acgcatttaa tggatgatgt agccaatttt
    95941 gcagattatg tctatgtgtt ggaaaaggga cgaatcgtta atagcgggga gccgcaagag
    96001 gtctttcaaa acattgaatg gctaaaagaa aaacaactag gcgtacctac tgccaccgag
    96061 tttgcagaag aattaatggc taaagggatg aactttgcta ccttgccttt gacagctgaa
    96121 gaattagcag acgctatcgt ccattatgct ggaggaaatg cccatgatga ataaattgat
    96181 atttggtcgt tatatgcctg gagattcctt gatccatcga ttggatcccc gagctaaatt
    96241 attagccagt ttttatttta tcggaatcat ttttttagca aataattggg aaacgtacct
    96301 tttgttggca tttttcacat tactttctgt cttcttgtca aaagtagatt tgaaattttt
    96361 tatccgaggc attcgcccat tgatctggct aatcctcttc actgtagctt tgcaggtctt
    96421 gtttacaagt ggcggagaaa cttattggag ttggggtgtt ttccatatca ctgaatttgg
    96481 tgtgatgaac ggcctcttta ttttctgtcg attcgtgttg atcattttta tgtcgactct
    96541 actcacgctt actacaccgc ctttggaatt atcggatgcg attgagtact tgcttcgtcc
    96601 attgaaagcc gtacggttcc cagtccacga aatttcattg atgctgtcga ttgctcttcg
    96661 ttttgtgcct acattgatgg acgaaacaga aaagatcatg aacgctcagc gcgctcgcgg
    96721 tgttgatttt ggtgaaggaa acctagttca gaaaatgaaa gcagtcattc ctttgctgat
    96781 tcctttattt gtcagcagct tcaaccgtgc ggaagatctt gcgactgcta tggaagcaag
    96841 aggctacaaa ggtggggaag gaagaacgaa atatcgtgta ttgcattggc ataatagaga
    96901 cacgatggtt atcattgctt ttgtattatt aactgtgcta ctgatctttt tgagaggata
    96961 aacgttcagt gggccgctat ctattctttt cttgctgatc tgtttgcaag aaaggatgcg
    97021 agcagtgtat gacaagaaat attagggtct gtggcagact aaggtcacag gcctctttct
    97081 tttatctgaa aagaaagaac gaaaaaattt tataaaaaaa tcattattcc agataacctt
    97141 ttaggaacaa aggagagaaa aacatggtac gttacaaagc aatcattgca tacgacggaa
    97201 caaattttaa tggattccaa aaacagccaa atgggcgtac tgttcaagaa gaagtagaaa
    97261 aaacattaca aaaaatggca aatggaaaag aaatcacggt ctttggttcg ggaaggacag
    97321 atgccggagt acatgcgatt gggcaagtaa tccattttga ttatccagag gagcgtccgt
    97381 tagaacgaat gcgtttcgcg ctagataccc agtcaccaga ggatattgct gtaagacaag
    97441 tagaaattgt ttcagaagac ttccacgcaa gatatttggt caaagaaaag acgtatcaat
    97501 ttcgagtgga tatagggaaa ccacgcagtc cttttcgacg ccattatgca agttattttc
    97561 cttaccctct tgatttggaa aaaattcagc gggcgcttcc tgatttatta gggactcacg
    97621 atttcacctc tttttgtgca tcaggaagct cgatagagga taaagtacgt acgatctacg
    97681 aggctaagat ggaggtaaat gaaacaaaag acgaattgtt gtttacattt aggggaaatg
    97741 gcttcttgta taagatgata cggatcttag taggaacgct tttgaaaatt ggcaatggcc
    97801 gattgcctga agatagtatc ccagatatta ttgcgaaaaa agatcgtaat gcagcaggac
    97861 ctacagcaca tcctgaagga ctttatttgt atgaagtgaa atatgaataa gaggttgtga
    97921 cagaagtggt cagcttcaag aaataagaag gaatttacga aaattacttc tcaaaatttt
    97981 gcaaaatttc ggcttatttt cgaaggagct acttctggaa caccgtgaat cagaggttgt
    98041 gaaaaagctg ccctgcatca agtaataaga acaaccaaac aaaaatagct cgtcatattt
    98101 ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt aaggaagagg
    98161 ttgtgaaatc agcgttttga cctgagtatt agagaaacaa acggaaaaat agttcctcat
    98221 attttgccgt ttgttgagct aataccgaag gtcagctgat tgaacaccgt ttaagaaaga
    98281 ggtcgtgagc ctgtcattta actctgagtc acaagtaaca atttcaaaaa acagcttctc
    98341 atctttgagg aatatcgtga cttgtggacg aggcgttgat aagtgaatca tctttttgtt
    98401 acctattttt tctgtaacca aaaaacgatg gaacgttgtt ttgattgccc ttaatgagga
    98461 cagtcggaac aggtttgtca tttttat
//
LOCUS       DAFEPQ010000003        86973 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.003, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000003
VERSION     DAFEPQ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 86973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.003"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(606..962)
                     /locus_tag="LP543_000203"
     CDS             complement(606..962)
                     /locus_tag="LP543_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBL2320402.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(976..1386)
                     /gene="spx"
                     /locus_tag="LP543_000204"
     CDS             complement(976..1386)
                     /gene="spx"
                     /locus_tag="LP543_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBL2320403.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1732..3918)
                     /locus_tag="LP543_000205"
     CDS             complement(1732..3918)
                     /locus_tag="LP543_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBL2320404.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            4136..4819
                     /locus_tag="LP543_000206"
     CDS             4136..4819
                     /locus_tag="LP543_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBL2320405.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4900..5619)
                     /locus_tag="LP543_000207"
     CDS             complement(4900..5619)
                     /locus_tag="LP543_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL2320406.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(5931..6137)
                     /locus_tag="LP543_000208"
     CDS             complement(5931..6137)
                     /locus_tag="LP543_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320407.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6382..6984
                     /locus_tag="LP543_000209"
     CDS             6382..6984
                     /locus_tag="LP543_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBL2320408.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            7125..8406
                     /locus_tag="LP543_000210"
                     /pseudo
     CDS             7125..8406
                     /locus_tag="LP543_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
     gene            8419..9147
                     /locus_tag="LP543_000211"
     CDS             8419..9147
                     /locus_tag="LP543_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2320409.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(9216..10739)
                     /locus_tag="LP543_000212"
     CDS             complement(9216..10739)
                     /locus_tag="LP543_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBL2320410.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            10991..12172
                     /locus_tag="LP543_000213"
     CDS             10991..12172
                     /locus_tag="LP543_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL2320411.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(12232..13860)
                     /gene="treC"
                     /locus_tag="LP543_000214"
     CDS             complement(12232..13860)
                     /gene="treC"
                     /locus_tag="LP543_000214"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBL2320412.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(14311..17022)
                     /locus_tag="LP543_000215"
     CDS             complement(14311..17022)
                     /locus_tag="LP543_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2320413.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(17032..17733)
                     /locus_tag="LP543_000216"
     CDS             complement(17032..17733)
                     /locus_tag="LP543_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2320414.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            17919..18518
                     /locus_tag="LP543_000217"
     CDS             17919..18518
                     /locus_tag="LP543_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL2320415.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            18599..19612
                     /locus_tag="LP543_000218"
     CDS             18599..19612
                     /locus_tag="LP543_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBL2320416.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            19720..20637
                     /gene="rbsK"
                     /locus_tag="LP543_000219"
     CDS             19720..20637
                     /gene="rbsK"
                     /locus_tag="LP543_000219"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBL2320417.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            20665..21060
                     /gene="rbsD"
                     /locus_tag="LP543_000220"
     CDS             20665..21060
                     /gene="rbsD"
                     /locus_tag="LP543_000220"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBL2320418.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            21060..22433
                     /locus_tag="LP543_000221"
     CDS             21060..22433
                     /locus_tag="LP543_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2320419.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            22790..24934
                     /locus_tag="LP543_000222"
     CDS             22790..24934
                     /locus_tag="LP543_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBL2320420.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(24986..26027)
                     /locus_tag="LP543_000223"
                     /pseudo
     CDS             complement(24986..26027)
                     /locus_tag="LP543_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
     gene            complement(26304..28106)
                     /locus_tag="LP543_000224"
     CDS             complement(26304..28106)
                     /locus_tag="LP543_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2320421.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(28120..28872)
                     /locus_tag="LP543_000225"
     CDS             complement(28120..28872)
                     /locus_tag="LP543_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2320422.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(28881..29243)
                     /locus_tag="LP543_000226"
     CDS             complement(28881..29243)
                     /locus_tag="LP543_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL2320423.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(29419..30291)
                     /locus_tag="LP543_000227"
     CDS             complement(29419..30291)
                     /locus_tag="LP543_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBL2320424.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(30342..30830)
                     /locus_tag="LP543_000228"
     CDS             complement(30342..30830)
                     /locus_tag="LP543_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBL2320425.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(30968..31759)
                     /locus_tag="LP543_000229"
     CDS             complement(30968..31759)
                     /locus_tag="LP543_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBL2320426.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GKSYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(31814..32422)
                     /locus_tag="LP543_000230"
     CDS             complement(31814..32422)
                     /locus_tag="LP543_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBL2320427.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(32415..32732)
                     /locus_tag="LP543_000231"
     CDS             complement(32415..32732)
                     /locus_tag="LP543_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL2320428.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(32888..33616)
                     /locus_tag="LP543_000232"
     CDS             complement(32888..33616)
                     /locus_tag="LP543_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBL2320429.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            33775..34656
                     /locus_tag="LP543_000233"
     CDS             33775..34656
                     /locus_tag="LP543_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL2320430.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERANRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(34893..35936)
                     /locus_tag="LP543_000234"
     CDS             complement(34893..35936)
                     /locus_tag="LP543_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL2320431.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(35939..36607)
                     /locus_tag="LP543_000235"
     CDS             complement(35939..36607)
                     /locus_tag="LP543_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2320432.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            36749..39085
                     /locus_tag="LP543_000236"
     CDS             36749..39085
                     /locus_tag="LP543_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2320433.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(39200..39511)
                     /locus_tag="LP543_000237"
     CDS             complement(39200..39511)
                     /locus_tag="LP543_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320434.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            39692..41167
                     /locus_tag="LP543_000238"
     CDS             39692..41167
                     /locus_tag="LP543_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBL2320435.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            41214..41798
                     /locus_tag="LP543_000239"
     CDS             41214..41798
                     /locus_tag="LP543_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBL2320436.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            41800..42219
                     /locus_tag="LP543_000240"
     CDS             41800..42219
                     /locus_tag="LP543_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBL2320437.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(42500..42784)
                     /locus_tag="LP543_000241"
     CDS             complement(42500..42784)
                     /locus_tag="LP543_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBL2320438.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(43016..43726)
                     /locus_tag="LP543_000242"
     CDS             complement(43016..43726)
                     /locus_tag="LP543_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBL2320439.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(43723..45195)
                     /locus_tag="LP543_000243"
     CDS             complement(43723..45195)
                     /locus_tag="LP543_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL2320440.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            45333..45806
                     /locus_tag="LP543_000244"
     CDS             45333..45806
                     /locus_tag="LP543_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL2320441.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(45959..46510)
                     /locus_tag="LP543_000245"
     CDS             complement(45959..46510)
                     /locus_tag="LP543_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL2320442.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            46615..47145
                     /locus_tag="LP543_000246"
     CDS             46615..47145
                     /locus_tag="LP543_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBL2320443.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(47628..48071)
                     /locus_tag="LP543_000247"
     CDS             complement(47628..48071)
                     /locus_tag="LP543_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2320444.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(48087..49247)
                     /locus_tag="LP543_000248"
     CDS             complement(48087..49247)
                     /locus_tag="LP543_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL2320445.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            49754..51454
                     /locus_tag="LP543_000249"
     CDS             49754..51454
                     /locus_tag="LP543_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320446.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(51556..52389)
                     /locus_tag="LP543_000250"
     CDS             complement(51556..52389)
                     /locus_tag="LP543_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBL2320447.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(52693..54858)
                     /locus_tag="LP543_000251"
     CDS             complement(52693..54858)
                     /locus_tag="LP543_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2320448.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            55118..57412
                     /locus_tag="LP543_000252"
     CDS             55118..57412
                     /locus_tag="LP543_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBL2320449.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            57405..58070
                     /gene="pgmB"
                     /locus_tag="LP543_000253"
     CDS             57405..58070
                     /gene="pgmB"
                     /locus_tag="LP543_000253"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBL2320450.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            58063..59070
                     /locus_tag="LP543_000254"
     CDS             58063..59070
                     /locus_tag="LP543_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL2320451.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            59092..60111
                     /locus_tag="LP543_000255"
     CDS             59092..60111
                     /locus_tag="LP543_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2320452.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(60392..61759)
                     /gene="nhaC"
                     /locus_tag="LP543_000256"
     CDS             complement(60392..61759)
                     /gene="nhaC"
                     /locus_tag="LP543_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBL2320453.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMVQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(61868..63289)
                     /locus_tag="LP543_000257"
     CDS             complement(61868..63289)
                     /locus_tag="LP543_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2320454.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(63532..65409)
                     /gene="tdc"
                     /locus_tag="LP543_000258"
     CDS             complement(63532..65409)
                     /gene="tdc"
                     /locus_tag="LP543_000258"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBL2320455.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(65699..66955)
                     /locus_tag="LP543_000259"
     CDS             complement(65699..66955)
                     /locus_tag="LP543_000259"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBL2320456.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(67067..67272)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            67535..68836
                     /locus_tag="LP543_000260"
     CDS             67535..68836
                     /locus_tag="LP543_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBL2320457.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(68929..70317)
                     /gene="ltrA"
                     /locus_tag="LP543_000261"
     CDS             complement(68929..70317)
                     /gene="ltrA"
                     /locus_tag="LP543_000261"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL2320458.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(70804..71403)
                     /locus_tag="LP543_000262"
     CDS             complement(70804..71403)
                     /locus_tag="LP543_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL2320459.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            71657..72538
                     /locus_tag="LP543_000263"
     CDS             71657..72538
                     /locus_tag="LP543_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2320460.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            72551..74161
                     /locus_tag="LP543_000264"
                     /pseudo
     CDS             72551..74161
                     /locus_tag="LP543_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
     gene            complement(74238..74897)
                     /locus_tag="LP543_000265"
     CDS             complement(74238..74897)
                     /locus_tag="LP543_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL2320461.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(75180..75845)
                     /locus_tag="LP543_000266"
     CDS             complement(75180..75845)
                     /locus_tag="LP543_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HBL2320462.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(75992..76597)
                     /gene="dhaL"
                     /locus_tag="LP543_000267"
     CDS             complement(75992..76597)
                     /gene="dhaL"
                     /locus_tag="LP543_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBL2320463.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(76625..77635)
                     /gene="dhaK"
                     /locus_tag="LP543_000268"
     CDS             complement(76625..77635)
                     /gene="dhaK"
                     /locus_tag="LP543_000268"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBL2320464.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(77610..78056)
                     /gene="dhaM"
                     /locus_tag="LP543_000269"
     CDS             complement(77610..78056)
                     /gene="dhaM"
                     /locus_tag="LP543_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBL2320465.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            78159..78479
                     /locus_tag="LP543_000270"
     CDS             78159..78479
                     /locus_tag="LP543_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBL2320466.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(78591..79460)
                     /locus_tag="LP543_000271"
     CDS             complement(78591..79460)
                     /locus_tag="LP543_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL2320467.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(79556..80860)
                     /locus_tag="LP543_000272"
     CDS             complement(79556..80860)
                     /locus_tag="LP543_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL2320468.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFI
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEEISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(80857..81366)
                     /locus_tag="LP543_000273"
     CDS             complement(80857..81366)
                     /locus_tag="LP543_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320469.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(81541..83676)
                     /locus_tag="LP543_000274"
     CDS             complement(81541..83676)
                     /locus_tag="LP543_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL2320470.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(83759..85414)
                     /locus_tag="LP543_000275"
     CDS             complement(83759..85414)
                     /locus_tag="LP543_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL2320471.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(85416..85730)
                     /locus_tag="LP543_000276"
     CDS             complement(85416..85730)
                     /locus_tag="LP543_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBL2320472.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            85900..86160
                     /locus_tag="LP543_000277"
     CDS             85900..86160
                     /locus_tag="LP543_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBL2320473.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            86174..86488
                     /locus_tag="LP543_000278"
     CDS             86174..86488
                     /locus_tag="LP543_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBL2320474.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT        25586 a        17713 c        15508 g        28166 t
ORIGIN      
        1 tgtttgttgt cttgaattga atcaagacgc cctacacatt tggtttgtct tactttgttc
       61 agttttcaaa ggtctacttc atcaggagtg ttccccgatt cgtttttggc tgccgtagca
      121 acttttatat catatcagag agacaagtaa ttgtcaactc ttttttaaaa gtttttttga
      181 tttcgttttt cgaaatctta cagcaactca tttatcataa cttatcattt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc acaagtggtt cttttttaac
      301 aactcagtta tcttatcatg tcatttctaa catgtcaaca actattttat atgtttttta
      361 aaatactatt ttattgcttt gctacttaaa acaacagaaa tgaatatatc atgtaaattt
      421 acacatgtca atatattttt aactctatat ccccacctag atttgaattt tgaccacata
      481 aattgagcat gtctctatag tagaaaaata aagtcaaaaa aagaacacat aacacgcttg
      541 atgctaagtg ttcgactgtt acaactgtat gaaatgctca gtgacttgat taaaacagtt
      601 aaagtctatt tatttaagat aagctgttcc atcaactgct cattgtatcc aataaataga
      661 acttcatcga taataatcaa aggacgtctg aggagattgg tatgttgtgt gcataaaaaa
      721 atcagttgtt tttttcctac acaggaaagg tcaatcgcaa tgttgtgaaa agcctcagac
      781 caagtcgcaa atagctgttc tgcttgcact tcttcaaaac tgcataattc ttctgcttct
      841 tctgtactta gatcgcgttc ttctaagtca acaaattgat aaggcaattg atggtgttct
      901 aaccattgtt tcgccttcag acttgttaca cagtgcgctg atccatagac tttcacttcc
      961 acgaaggctc cctccctatg aatttaagat gttattgttt tgtaaataga tcagatttct
     1021 ggcaacacat cgaatttcac gagggacaaa tgaccggatc tcatcttcat taaatccgat
     1081 tagtattctt ttttcatcaa tcaaaatagg tcgtctcaat aatgaaggat attctttgaa
     1141 taagacgatc atttgcttta aagttaattc gttaaaatct atgcccagct tttcgtaaat
     1201 tttcgaccga gtggaaataa tatcttgaac tcctccttca gtcgctgcca agatcttttt
     1261 gatctcactt tctgttaacg gttcggcaaa aatattcttc tcttcaaatt caagttgatt
     1321 cgcttgcaac catgctcttg cttttctaca ggaagtacag ctaggcgaag tatatacttt
     1381 tatcattttc tcactccttt ttaattataa tccaattcta cagtaatttc ttttaaaata
     1441 ccaattatta gctaataaaa tataaaaaaa atcaaaataa ctgattattt acgtttgaat
     1501 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1561 taagggaggt gtgacaaaat tcttgtcaca cctccctttt ccgaataaac tgtgttccag
     1621 aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat ttgagaagca attttcgtac
     1681 attccttctt ctttcttgaa actgaccact tctgtcacac cctctcttct atcactttcc
     1741 ttttttcaca cgactcccgg tttctttgat aaatctcgat actttcaatt ttcttgaatt
     1801 tttcatttga ggtacacata gatacagacg atttcttgct ctagtcatcg caacataaaa
     1861 tagtcttcgt tcttcttcta tcgtcgttac tggttctgct tttatttcat taaagtcgat
     1921 tactttcttt tctgtcgctc cgttataagg caaacttcct tcgttacaac caatcaaaaa
     1981 gacatttcgg taactaagac ctttactttt gtgaatgctc tggatacata gtgcatcaag
     2041 ttttccttgt ctgtaagcaa tcaagcgggt ttttaattct ttagctgtcg cttgggtttc
     2101 ttgcgataag gcttggagtc ttctgaaagt agcatgctgc tttacttctt ctcgtatcca
     2161 ttcttgatca ctctggtcga tttccttagc tatacgtggc agatgaagat agctttgaac
     2221 aagcgaatag atttcttgat gctggtctgt tttacgcaac gcttcctcaa actctcggat
     2281 ttctgcagat ttcgatttta aattggggct tatcgacaaa atataatctg caacggatat
     2341 attttgatat ttaggagaaa agtattgatc gcattcactt tttttcaggt agcagtctgc
     2401 catcagctgc cggagcaatt gctggtaggc ctgatttttc ttttgcagat tcagtgctgt
     2461 ttgccaatga acaatttttt gatagaaatg ttggaaagac ttgataccaa agataccttc
     2521 atatggacta tctgcttcca gtacaaaagg aatattcgtt ttcagcagag attggacaag
     2581 ctgctgcgtt tgtgaagctg atcggtataa tatgattgat tccttatatt tcccattttt
     2641 ttgaataaat gtctgaattt gtttgattag ccactgactt tctgctttag tatcagaaaa
     2701 caaataggct tccataacac ctgcttcttt ttgtgctgga atcatcgcct ttggtatccg
     2761 atcattttta ttatagctga tcaatctatt tgctccttgg acaatagctg cccgagaacg
     2821 ataattagtt aataagaaaa gagttttggt aggatattct ttttcaaagt tcttgatgta
     2881 tgctggctct gatcctctga atccatagat actctgatca tcatctccaa caacaatcaa
     2941 cttgatcctg ttttcctttg tcatccgttt cacgatttcc cattgaagtg gattagtatc
     3001 ttgaaattca tcaatgaata gataatcata tttttggccg atataatgct ggatctcctc
     3061 attctccaaa gcgcttttca gattttggag tacatcattg aactctagca attgctggct
     3121 tcttttttgt tcttggagtt ccgtgagcaa tccatacagt cgttctgata tcggctgtcg
     3181 tgcattctta tcaatggaat catatttttc tttaactaac tgaaccattt cttctactga
     3241 aaggttcata ttctgccaat agtctaaccg agaagcaatc gttttttctc catattgatc
     3301 aaatatataa agattatatt tacgtatcag ctgttgcaaa acagcattca ctttttgtgc
     3361 agtatccttt gaaaagtctc cttgtattcc ttgcattgct acttcaggaa attttcgaca
     3421 aatttcattg tatatcctaa aaaagatact atggaatgtt ccgatatgga cagcggtttt
     3481 tctctggctg aaattatcta ctcgtttttt tagttcattt gcagcgttcc ttgtaaaagt
     3541 gatcattagt atgcgttctt cgtctacttg tttttgcgtc atcagataaa cagcttttgc
     3601 acagattgtt tgcgttttgc cgctcccagc tcctgctatg attcgaagat gtttcttcat
     3661 atcatatgtg atagctgctt tttgttcttg attgaagcga ttataggaac gtttctccat
     3721 cattttaaag aaagagctgt ttccttcact ttcttttact tgtacttctt cctcaataag
     3781 tccgggtgct tcgccataag ccgcttcata ggtagcaatg atttcttctt gtagccgaaa
     3841 gacttgttga agctttttat acttgttctc ccagcgtttc aattcttttt gtaatcggag
     3901 aatttctttt tcattcaact gattgatttc cttcccatct ttcttctttg aatttgttta
     3961 tttcaagttg gttatttaaa gtttcgttgt ctattttttc tttatactaa cagacctttc
     4021 taaaaatgaa aatgggaagc gatatgattt tagttttctg aaaatttttc ctttttttca
     4081 atcgtttatg ctaagataag gaagacaaaa atattcataa aaggtgatga aaaaaatgca
     4141 gacttccttt cacgaggggc gtacaatagg acttttgctg acatttatcg gaggagccat
     4201 ggacagttat acttatatcc aatatggtgc ttttgcctct gctcaaactg gcaatatcat
     4261 tctatcgatc atccaagcat ttgacggaga atggctaagt gttggtaaaa aagtattgtc
     4321 tacccttttc tttttcttag ggattcttct aacaaagtat ctaattgatt attttagaaa
     4381 aaaagaaaaa catttctggc gcttgtttgt tctatattat gaagcagtga tttttttctt
     4441 tgttagtttg acacccgtcc atgagcatcc cgcattagta acgattttga ttgcttttac
     4501 tgcatcgatc cagtgggtag cttttgataa aatcaacggg cgtgcctaca ctaatttatt
     4561 tacaactgga aatttaaaag gggtagcaac gaacttatat gattattgta tttctaaaga
     4621 aaaagcagat tttgatcgat ttttccacta tctaagtgtc gttcttgcct ttatcgcagg
     4681 agctattgtc cttgttttct gtcatcatct gtttggcgcc cattcgattt tgatcgtttc
     4741 cattttattt ttgatcatgg ccctcactca gacatttcat ttatggcaat tttatcatac
     4801 gaatcggttc actatttgat ctcaatgaat caaaaaaact aagcaagatg ctgctcacag
     4861 agaaatatct gcgagtgagc aatttgctta gtttttatat tatttagttg taaagattcc
     4921 ctttgctttc gggttatttg tagtgtagcg gatcgactgg gcatctgcct cttctgtcat
     4981 tttctttgcc cgcatcttga tttcttgtac gatttgttct ccacatgccg gatcggctac
     5041 gaaatcatat ttgtccccat cgatcactat tttgggacat atatcaaact gttcatacca
     5101 ctcttcgtat cgtcgattga gttctttata gtaggaatag agttcaggat cttgttctag
     5161 ctgttcatat tcccggcctc tttttttgat tctcgctaac atagtttcaa aggatacacg
     5221 aatataaacc agtagatctg gtttcttttg tggcgaaatt tcttctaact ccttcagcat
     5281 cgtttctaaa agaccttcat actgctttac ttctacttca gaaacacgcc ctaaatctgc
     5341 attcaaatga aacagcatac tatcttcgta gatggagcgg tctagtacgt tgtcatcatg
     5401 gctgaaagca tctttgattg ccaaaaagcg tttgttcaaa aagaaaatct gtaataaaaa
     5461 agtatacttt tctggattag aataaaatag cggcaatact tcattatctt ccacgttctc
     5521 ataaaaaggt tttgtttcta aatcttgggc aagcatttca gttagtgtgc ttttccctgc
     5581 accgatcgtt cctgcaacaa tgatcacaat tatcggcatc cttcctgtaa taaaatctgt
     5641 tcttcatttt actacttcat ttatagaaat aaaagccttg actttatctg ctccttaaaa
     5701 gctacttctc taaaagtagc caaaagtaac tcctattcat gaaaataaag gctgtgacaa
     5761 aagtcttgtc gcagcccttt tttcaaataa acagtattct cctgccaaca cctcggtcac
     5821 aaggcaatat tcataaaaca tgaaaagctg ttttttaaca ttattccttg taactcagag
     5881 ctaaacggga gactcacaac ctctttatgc tctcaacatt ttatctgttt ttattcttct
     5941 attgtacttg atttgcgtac aactaactgc cagtgcagat tatgagcggt atcattgtct
     6001 tctactgcgc atttcatgac acgtatcatc acactatttt catagatttt ttctattttt
     6061 ccttttattg gtgattcaaa taaatcagat ttaaatgcat atgtttcgcc gacttcaaac
     6121 aattgttcat tttccataag aatccctcca tcacctttta taaggtcagt aattttttaa
     6181 cataaatcac tcgaaaaagc acaaacttgt cataaaaagt acttttcgta tcaaaactgt
     6241 cacttttttt ggtaaggttc ttggaaaacg aagatttttt taggagatca tgacggtttt
     6301 gatgtcaaaa ccacttttta tgaaacaaaa gacgtttttt atttttttta gcataaaatt
     6361 tgtggtggag gtacgtatga gatgaaagat aaagttgtga acaaattatt agaagcctta
     6421 ttcagcggag acaaatcgga agaagatatg gtgtatgtcc aagtaaaatt tgtcttagaa
     6481 gttttattga ataatcttgg aaaactagcg gtcgttctag tcttttctct ggtgacaggc
     6541 tcatgggcgg aaacaggaat cacctttctt tcttatatct gcatccgcag atatgcttat
     6601 ggattgcatt ccgactctga attcgtttgt ctgctgtgga cactacttta tttatggggc
     6661 gttcccttgg tgatgaaaca tctgcaattg actatctcct tcccaatgat ggtctttttg
     6721 ttaatcagct gctttttgtt actgcttcgc tatggatcaa gaggtacagc aatcaatccc
     6781 attgaacccg agaaacgtcc cccattatta aagaaggcga tcagcatgtt tctaattttt
     6841 tctctcatta cgttattttt ctcagcttct tacttttcta cctatttgct acttgggatt
     6901 gttttagaaa tagctacgtt gcttcctatt acaaactact tgatgaattt tggaggaatc
     6961 tttcatgaaa aaaacaatca ttaaacaatt aactaactta tctgactttt ttgtatctgg
     7021 ctgctgcttt ggtatgagtt ctgaattcaa aatccctgaa aaattaaaaa aataatacgc
     7081 caattgtgct ataatttggt aaaaatcaat gggagctgta ctttatgttt ttatggtcca
     7141 tagcgttgca atatttgatt tacacaattt gtatcttatt gattgaccat cacatccgat
     7201 acaaatatct gtttgcggga gtcattctga tttcttgtat tttgattgga tgggtctatt
     7261 cgattttagg gatttttact gctctaattg ctataggaat gctgatgctt acttttttct
     7321 tcttttcgac ttcgcgtcaa caattggttt acgcgctttc tttttgggtt attgacgtcg
     7381 cttttaggag atcacttaac aagcatcggc gatttttttg ttttgaaatc ttcggttatt
     7441 gaaccaggag attcattgca gtattttcac attgtcttat ccgctttttt atctgcttta
     7501 ttagcttttt gtttaaaaaa atcgttagag acttggtttc acactgtgga ccgccgtatg
     7561 atcggtacga tcgggacatt gatattattt acatactata tcattatttt ttatacccgt
     7621 ttttctggtg aaacacctga tgtcttagct ttgaatacga gttttttcat tctatatctg
     7681 tgtgttggcc tgatcatctt ggtttattat tggaaaatct ctaataagaa attagctgat
     7741 cagttattag agcaacaagt caggatgcaa caggattata tcaaagatct agaaaaaaac
     7801 tatcaagact tgagagaatt caaacatgat taccaaaatc tgcttttttc tcttcacagc
     7861 tatatttcag aaggcgactt agaaggatta cggtcttatt atgatcaaac gatattacct
     7921 acacgccaga tgatgaacct ttttccggcc aatctaagta tgttggataa tatgaaagtt
     7981 cctgagatcc gtagtttact gtcattaaaa ctcatgatgg cacaggaaaa agggttgatc
     8041 gtccagcttc attttcctga gccaatcgaa ctggatacga aacatacggt aaaccttgta
     8101 cgtatgcttg gtattatctt ggacaatgcg attgaaggag ctagtgctgc aaagaagaaa
     8161 aaaatcgaat tgaccatact caagaagaat cattccgtga tgattcgtgt gatcaataca
     8221 acaacaaata cattaccgtt aaaccagttg aaacaaaaag ggttctcaac aaaaacaaat
     8281 ccaaaaaacg aaggccttgg cttgtttatc ttggatgaat tgacaaagac caatccttat
     8341 cttttccttg aaacatcaat tgaaaaccag cgtttttctc aaacgattta tatacaaata
     8401 aaataaagga gtaatgatat gattcccatt tatatttgtg aagacgatcc aaaacaatta
     8461 gaagttttat caacggtgat caaaaataga atcatgattg aaaacttaga cagttatgtc
     8521 catatttcta cctctgatcc ggaagaactt ctcaatgcag ccaagatcag aacttcttct
     8581 tttggtattt actttttaga tatcgaacta gaagacagtg aaatggatgg tgtagcgatc
     8641 ggtaaattga tccgtgaatt agatccttta ggaaaaataa tctatgtgac ttcccatacg
     8701 gaaatgtcga tgaaaatctt aaaaagcaac atcgaaccga ctgattatat cgtcaaagaa
     8761 gatctttatg atttaaaaga aaatgtcgag catatcctga gcaaaatttt cgaagatttt
     8821 caaatgtccc aaatccaaaa agatatcttc aaaatagagt ttaatgatga gatcaaattt
     8881 ttaccgatca aagacatcca atatttttct acttcgctag gagcgccaca taaactggaa
     8941 gtccatttga ctcatgccca attacaattc tatgagaaga tcaaagaaat cgagaaaatg
     9001 catcggaatt ttatccgttg tcataaatcc tatgttatca atacacaaaa tgtgcggtcg
     9061 attaataaaa aaaccagaga agtcacgttg gcaaacggcg agaccatccc agcctctatc
     9121 cgtggattaa agaaattgat ttcataaaaa gaaccgaata gtttggaccg actaaatgac
     9181 cagccgatcc aaacccttcg gttctttttt gcctatcagt gcaaaagcat ttcctgtgca
     9241 atttcacgtc catccattgt atctggataa aatgttggcc aattctccat ttctttcagc
     9301 aatacttctt gactttcatt tcctgcatat aaatgaaaat gttcggagtc agccggtgca
     9361 atcgtgtgat cactgaattg aatatatcta aaagcgccac tttccttatc ttctgcttcg
     9421 aatagatacc ttacgccacg gtttcctgcc tcgtaggtca agatttcttt ccctacataa
     9481 cgataatttg aacgtttcgt tgttccattc ttaaacgtaa atgccatctc tttctcactg
     9541 atcttgattg aagcaatatc tgtttgatag cccttcgtat aatattcttt atattcttgt
     9601 gctgtcttat cctgattcaa tttttcttta tattcaaaaa caggatcaaa agtcccatct
     9661 tctacatacg tatctactgt ttgccaagtt cctgcccaat ctgaaagaga gcgatcttgt
     9721 acatcctcgt cttcaaaata accgttgtag acgctctttt cttttttggg ggtcaaagta
     9781 tcttctagcg ggttttctgt ctctgtcgtt tttttcaacg cttcgaggtt tgcttccatg
     9841 atagaaagat agttttctcc attttctatt tgttcattcg tcaatccttc caaaggattc
     9901 aatacttcta aagatacacc tgcttcgttt gctaacgttc tggcaatact atctttggcg
     9961 ttttcttcaa aataaatata gttgattcca tgttctttaa caaagtgttt cagttcagca
    10021 attctcgccg cagacggttc ttcactagaa gaaagaccag caatcgatac ttgattcaat
    10081 ccatattcta acgctagata gttgaatgca gcatgttgcg tgacaaaaga tctttgtttt
    10141 gcttgcttaa aggtttcttg ataaagctga tggagtgctt gaagtttctc ggtataggct
    10201 gctgcatttt ttgtccatac ttcctgtttt tctgggtaag cttcaatcag ctgatcacgt
    10261 atgtttgcta cctgcttgat tgctaaactt ggagctagcc atacatgtgg gtctagctca
    10321 tgtgtatggc cttcttcccc gtgttcatgg tcatgatcat gtccttcttc acttcccgga
    10381 agtaaaagca taccttctgt cgcttttatc gtatggacat tgccttcttg taatgttttt
    10441 tggattgcgg ggacccatgt ttccatgttt tcatcattat aaacaaagat atctgtatct
    10501 tggatctttg ccatgtcttt agcagaaggt tcataatcgt gggaatcagt ccctgctgga
    10561 atcaataact ctacttctcc ttcatccccg acgattgctt ttgtaaaatc atacatggga
    10621 taaaaggtcg tcatcacgct caatttttct ttactgtcct cggctttccc acttgccccg
    10681 caagcagtta acatacctgc taaaaccagc atggccaata tacctatcaa tcgtttcatt
    10741 tatgacactc ctcttctcat cttctttcat ttgaatgttc cgattcgctt ttcgcaaacc
    10801 gtaatagtta cgttttagca acaggattta atttatcata tggttaaaag aactgtcaag
    10861 cgttttctta ttttaattga tgagaaagtt tatatgaatg attgacgata tttttgcaat
    10921 caacctctat cattttcctt ttatacccgc ttttgtctat aatgaagtta tagataaagg
    10981 aggaaaaaga gtgaaaaaag aaacagatac aacgaatcgt ttgatccgct ttttaggagg
    11041 aaaaacctct tactatgttt taggaatagt tatcttactt gcgctgatca tttatttttt
    11101 ccatcagatc tcgtttatat tcaagcctgt cggaatcatt atcgctacta tcttgccccc
    11161 attattgttt gcactgatct tgtattatct tttggaacca gtcatcaaac gtttgagcaa
    11221 gcacctttcg aggaacttgt cagtcattat cgtttatgtg attattttat ttttattggg
    11281 gcttggaagc atatggctta ttccttttct tgaagaacag gcaaagacat tgatcagtag
    11341 tttgccagat ttgtttcaag attttcaaga gagtctccga caatttttgg agaaaacacc
    11401 ttttgccgat tcatttaatc aatttttcgc ttctattgac gacgtgacgt ctgatatagc
    11461 tggatttatt ggaaattatt gggaaagtgg cgcacaacgg atcagcaaca ttttttcaac
    11521 tgttacagct atttttatta ctttatttac tggtccaatt gtcgcttttt tccttttgag
    11581 agatcctcaa aaattttatc agtcagtttt agcgattgtc ccaccagcat ttcgtacaga
    11641 ttttaaaaat ttgacaaaga ttgctaatca acaattgggt tcatttctaa aggggcaaat
    11701 cattgcttca tttatccttg gagcagtcta ctgggtttgt ttcttactga tcggattaga
    11761 atttgctagt gttttagcta tttctgctgg gttgttatgt atcattccat atattgggcc
    11821 gtttatcgcc ttctttccag gtttgatcat tgcattccaa gactctactt ttatggctgt
    11881 caaatttgtc attgtctggt ttgccgtaca acttctccac ggtgacttgg tgattcctcg
    11941 agtaatgggt gatcgtttac agatccatcc gattacgatc ttaatcgttt tacttgtcat
    12001 gggtgatcta atgggaattg tgggcgttat ttttggtatt cctatctata cgttagtcaa
    12061 attactcgtt acttttattt tccgtaaatt caaacaacgc tacaataaat tttatggcga
    12121 taaaggagag tatgaaaact cttcattttc tgaagaagat tattttaaat aaaacacaaa
    12181 acgtcgatct tacgagaata gctcgtagga acgacgtttt gtgtttttgt tttaattttt
    12241 cagcaaagca attgcctggt atggttttag ctccagactt tgatgaagaa cagtatcgga
    12301 ataattatcg ataagaatat tggcttctgt gaatccttct gggatggcga tccttgtatc
    12361 tttatcatag aaatttgtta atacaagcag ctgctgtcct tcaaattggc gaagaaagcc
    12421 ataaacttcc ggatgatttg cttcataagc atgataatct ccttgtgcaa cgattggata
    12481 tgtttttctc agccgaatca atttcttgta ataggacaaa atagaatctt cagaagatag
    12541 ttctttttct acattaatct cttctagctt gcctccaact tttaaccaag gtttccctgt
    12601 agtaaatcct gcttgttctg tgtctgacca ttgcatgggt gttcgagagt tatcgcgaga
    12661 ttttgctttg atccgacgaa acgcttcttt ttctgtgaat ccttgttcaa ggagtaactt
    12721 ataagcattc aaactttcta cgtcaatata gtcggaaatc gatgaatagt ctgggtcgat
    12781 catgccgatt tcttctccca tataaatata gggcgtccca cggttcaaat ggatcatggt
    12841 tgctagcatc tctgctcctt gttttcgata ctgatcgata gctacaaatc gattgagcgc
    12901 acgtggctga tcatgattat taaaaaatag agcattccag ccgttaccct cactcatctt
    12961 ttctccccat gtatgaaata gtcgctttaa ttctgcaaaa tcaaatggag gagtagacca
    13021 tttattccca ttttcataat cgactttcaa atgatggaaa ttaaagacca ttgataattc
    13081 ttctctacta ggttctgtat acaagataca attttcgatc gtcgtagagc tcatttcacc
    13141 gactgtgatg atagtggaat ccgtcccaaa cgttgcttgg ttcaattctt ttagatacgt
    13201 atgggtgatt ggtttgtccg tatattcagg tttgccatca ttttctttat tatttttcaa
    13261 ttgttcgtct ttaccgataa cattgataac atcgaaacgg aatcctttta cccctttttc
    13321 catccaaaaa cgaacaactt tgaatagttc ctctcttact gcttggttcc gccagttcaa
    13381 gtcagcttgt gtccgatcat ataagtgcaa ataatatttc cccgtatctc caaatggcga
    13441 ccaagcactt ccaccaaatt ttgataccca atccgttggc tcttctctta aaatgaagaa
    13501 gtcctgataa tgctggtccc ctgctagtgc ttttttgaac cattcatgtt ctgtcgaaac
    13561 gtgattcagc accatatcga gcatgatttc tatccggtat ttctttgctt cggctattag
    13621 ctgttcgaat tcctccatcg taccaaagat aggatcgatg gctgtgtaat cagagatatc
    13681 ataaccgttg tctttttgag ggcttggata aatagggttt agccaaatca tgtcgatgcc
    13741 tagatcattc agatacggga gtttttcttt gattcctttt aaatctccaa ttccatcacc
    13801 attactgtct ttgtaagatt tgggatagat ttggtaaatg actttgtctt taaatgtcat
    13861 ggatatttac ctccattcaa ctgatttatt acctaccaca acttatatat acaagttatc
    13921 atttattatt ataactgaac cgaaagcgtt tgtaaaattt atctttatac attctaagag
    13981 gtctgttgcc caataaaaaa caagagatca gacatgtatt tgtctatcct cttgtttaaa
    14041 atagcaaaat attcttgttt cttctataaa tggtgttcaa aagcttttat ctacggcaat
    14101 cagtcttctc aaatgtaaaa catgaggagc taattttatt tgagtattct ttttgctcga
    14161 tgcaggacgg cttttttaca acctctgatt catggtgttc aatcagctgt ccttcggtat
    14221 tagctcaaaa aacggcaaaa tatgaaaaac tattttgccg tttgtttctc taatactcag
    14281 gtcaaaccgc tgatttcaca acctctgttt ttattcattc gatttcaacg catcaatcat
    14341 atcgatatgc tttaatttga aatgggtaac aatcatcaca ataaccgtaa atacgatagt
    14401 taatcctgct gataagacat aagcccacca tgtgatcact aatggaaaga tgacattctc
    14461 catagacgct tgttgcaaga tgaagtcagt caatatatat cctacaccaa atcctccgat
    14521 gattcctaaa agagtaaaga tgatattttc tcgaacaatg tacatcgtga cttctttatc
    14581 aaaaaatcca aggactttta ttgtagataa ttcgcgtaca cgctcggaaa tattgatatt
    14641 agttagatta tacaagacga taaaagccag cagtcctgat aatacgacaa agattaagac
    14701 aaccgagtct aagttcgcca ttgattcttc ttgcgtttcg atttgtttgg acatgaaaga
    14761 cgtatttgtc acagcgtctg tggcaagcag tttctcggaa agcatttctt cttcttttgt
    14821 tgtcatctta tctgtacgca gcaaaaatgc attatccatc atcgattgat cagtaactga
    14881 agcataatat tcaggggaca tataaataaa gctacctaga tagtttttca cgatttcttt
    14941 tacttcgact ttccattctt tttgatcttc atcataaagc gaaatatgat ctcctgtaga
    15001 taagtcaaat agttctgcta ctttttgcgt aatagcgatt cctgttttag gcaaggagcg
    15061 tgggttctct tcaagatcag ttaaatggat aaaacgatcc aaatcagctg ttttacttgg
    15121 tacgatcact gtcagacttt gcgaagcatg tccttttttt ctcaattcta cggtttgagt
    15181 atgaatagct aggatctcat ttactttttc ttgcttctgc agcatatccg ctgctttttg
    15241 acgactatct tccgtggatg aatccaatgt aacgattgct tgatagtcaa tgatcgttcc
    15301 aaattgtttg gctgaaacag aacttagaga atctttcaat cccacgcctg caaccatcag
    15361 cccggcacat ccagcaattc cgataatcgc cataagcatt cttgacttat atcggaagag
    15421 atttcggtaa ctgatttttt gattaaagct aaggcgtgac cacagcggag tgatgtattc
    15481 aagaaaaatg cgtttccctg gcttcggtgc tttcggttga agcaatgcag caggccgttc
    15541 tcttaaatct ttgaataaaa cgatcattgc agcgccaaat gcggcaatga agaatgcggc
    15601 agctgcttgt aaaatcggcg gccagtcata aaaaataagc gcactcccaa tatcataccg
    15661 ctcgttagac agctcaaaaa tgatccttgg caaaagattc gttcctaata tcgttcctaa
    15721 aacaatacct atccctgagg ctagacttgc atagattgca tatttttttg cgatttcaac
    15781 tttcgtataa cctaacgcct ttaatgtccc gatttccctg cggttttctt ctaccatacg
    15841 tgtcattgtc gtaaaggtga tcaatgcggc aataaagaaa aagaaaacag gaaaaacatt
    15901 ggcaatcgca gcgatacgat cagacagatc accaaattct tgaaaaccga cattttctga
    15961 acgttcattg tataggtatg atggtttagt taaattttca attttttctg catttcgatt
    16021 caattcttcc tctgcagagg ccagttcacg ttctttctca actaattgcc tttcttgttc
    16081 agcaagttga gcttcttgtt gagataatgt ttgaacagcg attgttcgtt gcgcttcagg
    16141 aagctgctgg atagttgctg actgttgcgc taactgtttt ttagcatctt ctaaccgtgt
    16201 tcttgcttgc gttgtttgtg ctttgccatc cgctattttt tgctgtgccg gttctaattc
    16261 ttcgttggcc gattttttca gctcttctaa gcgttcttcg ggtcgatctt gtaaatactt
    16321 ttctgccttt tcttgatttt gctccatttt atctttgtaa gctttgctgt acgtattcaa
    16381 attctttacg tttgtaaaat caagatataa gacagaataa atatctgttt taaaaacgga
    16441 ttcaggtaaa tagacaaaat aatcgacgct accatttccg acgttcgcgt atcctctctc
    16501 caaattgttg atgaacaatg gagaacggac aaagcccgtg atggtatact ctttttcttt
    16561 caattcatct gaatcaatct gataaatatc accgatttta tacccttcat cttccgcttt
    16621 ttcatccaat acaagctggt ttggtttttc cgggagtttt ccatcagtta attgtagctt
    16681 gttctgcttg ttatttttgt tataagaaaa tatttgaatc acttcgtttt tgccagtatt
    16741 ggaataatag atctgttttc ctgtttctac ttttgcatct gggatctttt ctgcttgagc
    16801 aatatctttt tgtgtcaagc caccagtaga aacgatttgc aaatcagaaa gtcctgctgt
    16861 accaacatag tgatccatcg ttttttgcat gacaggtcca gcagttttga cacctacaaa
    16921 taaaagtgtt cctagcatga caataaggat gattgcaatg aatcgtcctt tagattgccg
    16981 aatttcgcga aaacctgctt taagatatgt tcttgttttc ataaaatccc cctaccatac
    17041 aatctcatca atggatgaag gatgatcatt taattcaaca gaccttactt tagcatcatt
    17101 aatgtgaatg actctatcag cgatgggcgc taaagcagaa ttatgtgtaa tgatcaaaac
    17161 ggtattgccg tgttgtctgc tagcgttttg caataatttc aatacttgct tgcccgtttc
    17221 aaaatccagc gcgcctgtcg gttcatcaca taaaagaagt ttgggatttt tagctagtgc
    17281 acgagcaatt gaaaccctct gctgttcgcc tcctgataat tgggacggaa agttattcaa
    17341 tcgatgagct aggcccactt gcctcaatac ctctacagga tcaagcgcat caggagaaac
    17401 ttccgttgcc aattccacat tttcttttgc tgttagatta ggaacgagat tgtagaattg
    17461 gaaaacgaac cctacatctg ttcgtcggta agcagtaagc tgtttatctg aaaattgcgc
    17521 aatatctgtg tcatcaataa tgatctgacc ttcatcaggt gtatccatcc cacctaaaat
    17581 attaagaatc gttgactttc ctgctccact tggaccaagt ataatcacta attcgccttt
    17641 ttcaattgaa aaagaaatat cgtcatttgc tgtaatggtc gtttctccca ttttgtatcg
    17701 tttgtattca ttttttactt cgacatactt cactctgatt cctccatttt cacgatattt
    17761 ctcgctatat ctatgcatcg aatgcgtttt ctcacactta tttattttac cactgaattt
    17821 ctctagacta aagaaaggaa tcctttaatt tagttgacaa ataaacgatt tatgatttac
    17881 ttacataaaa aacattgtta tttgaaagaa ggaacatcat gaatgattta aaggaaaata
    17941 tgatttttga tttatcaaaa actgcttttg tcgcgatcga catgcaaaaa gagatcgtaa
    18001 ataacggtac actctctcca cacactgcag catctattct gacaaacaat gatttgttag
    18061 taagaacatt gaaacgtacc gctgccttga aagttttagt gaacgtagac atctccactt
    18121 tcccttattt gagtcaacaa actgatatgg gtggaatgac tgcttctgta cctccagaat
    18181 tcactgattt acttttgaag gattctttga aagatacaga taatatgttg actatcacaa
    18241 aatacaatcc aagtgctttt tttggtactt ctcttgatct tcaacttcgc cggcgtggaa
    18301 tcgaaactat cattttaagc ggtgtagcga caacaaacgg tgtatatgct actgcattag
    18361 atgcctttca acacgggtac catatcgtgc ttgctgaaga tgcttgcagt gatcgtgaca
    18421 aagaatccca tcaattgttc ataaaaaaga tatttccgaa aactgcacga gtacgctcaa
    18481 cgaaacaaat aatcgaagct atccaacaaa gcaaataagt actggcacat gtgattttca
    18541 tttacttctt tttgatttca tgggaaaata accacaggat ttagatagga gtatgaacat
    18601 ggtgaaaaaa aagatcacaa tcaaagaagt cgctaaactt tccggcgttt caatcacaac
    18661 tgtctccttg atattaaatg gaaatacgga gaaattcagt ccgaaaactg ttcagaaagt
    18721 tttagctgcc aaagaagaat taggctatca gcctgactat tttgctagac aaatgattac
    18781 caaggaaacg aaaacaattg gtgtactcgt tcctgatatc acgaatccat tcttcagtac
    18841 actgatgcga ggaattgagg atatcttgta taagcagaat ttcgtcacga tattatgtaa
    18901 tgctgattct gatcatcaaa aagaaatcga atatctagcc gagctcacac gtcgaggtgt
    18961 ggatggattc attatcgcga cttctgctgt ctctacagat gcaatcaacg aaaatttgaa
    19021 aaaacaaggc cggcccttca ttgttttgga tcagaaaaaa tctgaaggat ttagtgatgc
    19081 cgtacgaaca gacgattttc gcggtggtta cctagcgggc atgcacttgc ttagtctagg
    19141 acatcaaacg atcgcccttg tctatcctga aaatccaccg gaaaatgtcc acgctcggat
    19201 tgaggggttt aaatctgctc ttgatgttta ccaaatccct cacgaccaac tgatcctctt
    19261 acctacacag ttctcaaaac aaggtggcta ccagatcaca gcagaacttc ttgattctgc
    19321 tgcaactggt gtgtttgctt taaatgatga attagctttt ggattatatc gcggattaga
    19381 ggaagctgga aaaagcatcc ctgaagacta cagcattatt ggctatgata atatagatat
    19441 gtgcgagtat atcaagccga aactgacaac gatcgcacaa ccaatcttcg aattgggaca
    19501 aacatctgcc aagttattgt tagatcggat ccaattccca gaaaaagaat gggaagaaaa
    19561 aagattacct gtacgattag aaaaaagatt ttcaactgca cccttgaaat agctttcaag
    19621 gtgtgctata tttttatcga actattaaaa cgttttacta aaacgtttta aaaaataatt
    19681 ttcccatcaa aataaatgag atagttggag gtttgtaaaa tgaacacggt tacagtaatt
    19741 ggaagtatta atatggacac tacccttcgt ttaaccaaca tgcccaagcc aggcgaaacg
    19801 atgcatgcgc atgagatttt tcacgctggc ggaggtaaag gtgccaacca agcagtggca
    19861 gccaaacgtt caggcgcacg gacatctttt attggaggcg ttggggctga cagcgaagga
    19921 caacagttat tagatctact gacaaaagaa aacatcgata catctgggat agctgaaatc
    19981 caaggcgcca caaccggaca agcaatgatt atggtagacg cagcaggaga aaatagtatc
    20041 ttgatccatg caggggcaaa taatgctttc catgaacaag aagtcctaaa aaacaagcaa
    20101 ctgattacaa acagtgactt tattattgct cagtttgaaa gtagcttaga tgcgacgatc
    20161 ctagcttttt ctattgcaaa agacgcagga aaaacaacga tcctgaatcc tgcaccagct
    20221 cgtgaaacga ttcctactga actacttgaa aagacagata tcattatacc aaatgaaaca
    20281 gaaacagaaa tcattacagg aatcagagtg acagatcata acagtttggt cgcagcagcc
    20341 gaaaaactcc atgaattagg tattggaaca gtaatcatta cactaggcag tgccggcgct
    20401 ttctatcata cagaaaaaga acacggtatc gtccctgctt ttaaagtgga tgccgttgac
    20461 acaacagccg caggtgatac atttattggc gcattaagca gcacacttca gccagacctt
    20521 agtaacttga aagaagcgat cctttatgga aatctagcct cttctgttgc tgtgcagtct
    20581 tatggtgcgc aaccttctat cccttaccgc gaagctttag aacaatcagc agtctaaaat
    20641 taacaatgag aggaagaaac aaacatgaaa aaaggaaaag taatcaattc agatatttca
    20701 cgagtaatcg cacaaatggg gcactttgat aaattaagta tcggggatgc aggcatgcct
    20761 gtcccctttg gtactgaaaa aatagatttg gctgtctcaa acggtcttcc aagtttttta
    20821 tctgttttag aaaacgtttt agaagaactt gaggtacaat cgattgaatt ggcggaagaa
    20881 ataaagacaa tgaacccgga aatattggaa aaaatcaaat tattgttacc agatacaccg
    20941 attagcttta tcccacatga agaaatgaaa aaagagttga ataactgtca tgcatttatt
    21001 cgtacaggcg aaatgacacc ttacgccaat attatattag tgagtggagt ggtcttctaa
    21061 tggaaacgac aatgaatcct gaacagatgg tactgtccga gcagattcat atgctgattg
    21121 aacagttggg aggaaaagac aatattgatt ctgtccttcc atgtgcgact cggcttcgta
    21181 tcaaattgaa aaaacagcca gtagccaatg cagaaaaatt aaaacagcaa atcccttcca
    21241 ttaagtattt cattttactc gatagatatg cagaaataat catggatcag ggattggctc
    21301 ctgcttacaa ggaattgacg cgtcaaatcg atcattcagc tccgaaagaa aagctaaatc
    21361 cgatcgaaaa actatttggt ttgatatcgg aaatctttac gccgatcatt ccgctgtttg
    21421 ccggttctgg tatattaaaa ggtctggttg ttttagcgac tacgattggc tggcttagtg
    21481 aaacaagcgg aacttatcat attttgtctg ctgcgtcgaa tgccgtcttt tattttctgc
    21541 caatctttct tgctttttct gctgccaaaa cgtttaaagt gaatggatat attgcggctg
    21601 tcattgctgc agcactgatt gaaccgaatc tgacctcttt actttcagac agtggcaaag
    21661 cagctactga ttttctgggg attcctgtag tactgatgca gtatacgtct actgtcatgc
    21721 cggcgatttt aggtattttc ttttattctt ttgttgaaaa attcctcaaa aaatatattc
    21781 gcgaaaatat gcaattagta tttgtacctt tactttcatt agttatcacg gttcctttga
    21841 cgttaatggt attcgggcct gcgggtgttt accttggcga aggtttagct gccgcaatta
    21901 cttggatgat ggatattaac ggaccgatca gtggcgcaat catcgctggt ggttggaata
    21961 ttttggtaat atttggtatc caatgggctg taaaccctgt catgatcagt aatatctctg
    22021 cttatgggtt cgaccgaatc gttccgttga caggagcagc caattttgga atggctggtg
    22081 cagctttagg tgtatttctg cgaagtaaac gttcaaaaac ccgttcgatc tccggttctg
    22141 cttttgcttc gattttactt gcgggagtga ccgaaccaac tgtttacgga attgcgatcc
    22201 cattgaaaaa accatttgtt gctgcttgca ttggtgcagc tgctggtgga gctgtaatgg
    22261 gatttgccca agtgaaagcg atcgcttttg tatttggtag tctgaccaca ttgcctgcat
    22321 tcatttccgg tacttttttc tggtacttag caggactggc tgttagtcta gtcgtagcaa
    22381 tgatcactac tctcgttagc gggtttgatg aagacctaat gtcttatgaa tagttaatga
    22441 aaaagactga agtatgagat gtttatcaca tatttcagtc tttttctttt taggatcatt
    22501 atttttataa atttttgtga tactttccaa actttggtat aatttacata tgcataacca
    22561 tttttatccc cacttgttta tccccaaaca aaggtggtct atgcaataaa atgaacgtca
    22621 aagaaaaacg aatgaatgct cgttgcttat ttacaaattg ttttatttag aatacacaga
    22681 cagatttatg aaaagaaaaa gcttttcgac acgattatct gttccaaaaa atgtctaatg
    22741 aatgctgaat aagacagaaa atcaatgaaa ggaaaaggac tagtttatta tgaaaaataa
    22801 aaaaacgata cgtttgctag ctgctacttg gctgattgct ccgtctgttt tgtcgaccaa
    22861 taccgtattt gccgtagata atcaaatagc tgatcaaaca gtcacaacag atacgactac
    22921 atccagctca gctactagtg aaacaacgga ttcttttaca gaagacccaa ctggaacaac
    22981 taactcaaca acaggatcga ccgagccaac tgaaattgat ccggctgatc catcagaaaa
    23041 tgaaaagcca gccgagacat attcccttac aattgctcct tctttgattg gtaagatcca
    23101 actgactgct tcgacagcgg attcccctga acagcaacct ttggatatta cggaaagtgg
    23161 aacggtagaa ggtttattag cggaaacgaa ggtaacttac actattacgc cagctgaagg
    23221 tgaacaaatt gcttcagtcg ctgttaatgg tgccaaagaa gtaaattatg atgcaaagac
    23281 ctttacagta ggtacgaaca atttatctat tactgcagaa tttaaagctg gaccggatac
    23341 ttcaacagag aaaccaactg atcctagtga agaagtccct agtacaccag atccggaaac
    23401 gccaaacaaa ccagatgtgg gtaataacaa caataataat ggttcaaata attcaaacaa
    23461 taataacaat aattcgaaca gcgggaattc ttcgaatcaa acaaacccat ctggatcgaa
    23521 taacaatgga aatactcaga gagaaccaaa ccatccaaac aatggttcat cgaatggcgg
    23581 aacgaacaat gcttcatctc gtccaaatac agcaaatcaa agcggtatag agaatccatc
    23641 ttctgcttct tctgattttg ttgtaaaatc accaattgat gctgtattgc ctacaaatac
    23701 aacgaatgtc caacaagcaa tcgttcgaga agcattcaag catcttggaa aaccatatgt
    23761 atggggagca aaaggtccaa atacttttga ctgttctggt ttgacttatt acgtatatat
    23821 gaaagcaacc ggtcattata tcggtggctg gacaggcgaa cagcaatatg caggtacgca
    23881 gattccagta agtcaggccc aacctggcga cctcgtcttc tggggacctt cttccggtgt
    23941 tactcaccat gtaggtatct atattgggaa cggacaattt atccatgcgc ctcaaccagg
    24001 agataaagtt cgagtaactt ctatttcaga tttcacgcct gattttgctg tccgagtaaa
    24061 cttagcaggt ttacctgctg cttcaggttc gctgacaggc ggctcatcta ttttagatgg
    24121 attagatggc tcgttccatt tcagtcaaaa ccaaacaact gatcagttct tgaaaaagat
    24181 tgcagatgat gcacaggaaa tcgggcaaaa agaaggaatc tacgcatcag tcatgatggc
    24241 tcaagctatc cttgaaagtg gatctggaaa cagcttatta tcaagcgaac caaatcataa
    24301 cctttttggt atcaaaggat catataaagg aagcagcgtg accttcaata cacttgaaca
    24361 agatagttcc ggacaaagct accaaatccg cgcacaattc cgtaaatatc cttcttacaa
    24421 ggaaagtttg gaagactatg ccgacttgat caaaaatgga ctgactggca atcctgattt
    24481 ctacaaacca acttggaagt cggaaacaaa agactacaaa gaagcaacca aatatttgga
    24541 aggacgttat gctacagatc gccaatattc acaaaaattg aacgcgatca tcgaagcgta
    24601 cgacctgaca aaatatgatg aaccgaaaaa agaagcagca acaactgaag aagcaaacgc
    24661 tgaaatcctc aatatgtcta agcgattcga tgtgccaatc aaatggacaa cagatggcgt
    24721 gccaatcaca ggattagaat tttcaacgat aaaacgcgtc aacaacgctt cttccatctt
    24781 ccgtgtcgct tcaatctggg atttgtggaa caattttacg gcaagacaga taccagaaac
    24841 aactactcgt acagttaaag ctgcaactgt tcctgtacta accttgttaa gtttagacaa
    24901 atcattctgg cttgatctgc ctttaaagaa atagaataaa ttaaaaaggg gatgtgacac
    24961 tgtcacactc cctttttaat ttattctatt tctttatatt tattttgtat tccgttacaa
    25021 actcgcgtac ttggttaggt tccagagtga catctccaaa actttcaagt cgctccgctc
    25081 ctggcagttc ttgagtctca aatgtgatac cgccatgatt tactaattta ttcccgcgca
    25141 tttccggtcc atcttctcca aaatttgctg taaaaatcac gattcctgaa gcatctgttt
    25201 ttacttggat cgatactttt tcatctgggc ttgtcaattc cgcagcattt agtaaaccag
    25261 attgtttttt caacaaaaat ggatgatcga tgccgcccac tatcattgat tggcgaaaat
    25321 cactggcaaa gacctcacct aattttttag gacgttggaa gtcaaatgct gtgccactta
    25381 ctgaaactac ttctcctgta ggcgttgtat ctttattgag cgtagcaaat aatcggctat
    25441 tcaaccaaag tatatgttcg tcgattgact gtgctggatc accggttaaa ttgaaataga
    25501 catgatttgt aggattgaat actgttttgc ggtcactgat cccttggacc gtcactcgcc
    25561 atgtatcatc gttcgtcagc gtataccgta cttctacatc taaagttcca ggaaatccgt
    25621 tttccatatc tgggctagtc tgatagaaaa tgaccgatgc ttcgttttca ccattgatga
    25681 ctgtataatg ccactgcttg gcttcaaagc taggtgcacc accatgtaaa ccaatgacca
    25741 gtgtccgggt ctgtttttac ttcgaacgtt tcatcatcta atttgaatgt gccattcgta
    25801 atccgtccag ccatacgtcc gattgaagca ccgatataag ggtctttttc tagatattct
    25861 tttgcttcat caaaacctaa gatcaactct cgtttttcat tatcgattat tgtttgaaaa
    25921 tgaacaaccc gtgccccaag atcactgaca gacaaagatg tcccattttg attcgtgatc
    25981 gtaatcaagt gatagttttc gccaaaattt tgttcattga tttccatgtt ccttgcaccc
    26041 tcttttcttt catttgcact gattccagca tacatggaaa ccgttttctt aacagcttat
    26101 tccgtgagta acgagaaaaa atttactata ttctaataaa agaccgtgaa aaaaaggttg
    26161 tgacaaaaat attgtcacaa cctctttttc cgaacaaacg gtgttcaaaa gcttgcatct
    26221 gcggtaataa gcccaaccaa acgaaaaata tgaggagcta tttttgcttg gctgttctta
    26281 ttacttgatg caggacagct ttttcacaat ctctttttca atacgattcc agtatataac
    26341 catgtcgtca gtacatagta gatgaaatac aatacgaaga atatcgtaaa cggaacccaa
    26401 atatctttgt atggattttg catgaataga ccaaacattt gtaatccgaa taagacatgg
    26461 actacaccta agattcctgg tgctaagaat aacacgccta cttctctacg gatcgattgt
    26521 ttaagcaacc cgcgtcgtgt tcctatcttt tcaagcattt cataacgagc aatgtcactc
    26581 ttgcttcctg acaagatctt gaacatcaaa cagctagcaa gcatggtcaa gaaagctaaa
    26641 cctaggaaga atcccataaa ttcaaaacca gagaatagac cattatacac ttcatacatt
    26701 tcatatttct ggttgctgta ctctactttt tgcaaagatg gattgttcat tttattttga
    26761 gcaacaagtg tttcgattgg tttcaggttt gcttggaagt cttttacctg aactagctgt
    26821 aaagtggatt gtggcaagtt cagttgatca aattctgatt gactgactac cttgatttct
    26881 ttttcttgct gttccggcaa ttcatagctt cgaagttcct cttgtacagt cagttcattc
    26941 atttttttag caggaacttt ttctttcgtg atcttcggcg gtgttccatc ttcatgtttt
    27001 atcatgatca atggttgagc cgcaaattca cttgcattat agtaaatcgt tcccgcatct
    27061 tctttttgag tataagaagc agaaagtgtt ggtgaaagtt ctttgatttt ttgctgatct
    27121 tctgcccgag gattattcat cactagatca tatccagaat tgttttctgt caagtctgca
    27181 atttcatttt taaaacctaa tcctaccgtc aaagcgccca gtgccaaggc aaacagcata
    27241 gcgaccattg agagcatttg tgtatattca cgaatacgaa agtttaactg tgacaatgtg
    27301 aaattgttca attttttcaa tgaaatctgt tctgatttct ttagtaatga taaaaagaaa
    27361 atgatcacag aatggaaaat gaaataagtt cctaatacga tcgtaactaa ggctacccct
    27421 ataccaaaga cttgataggt ccccacgtgt ttcatcatat agtaaccgca gcctagaaaa
    27481 gcaattccca gtactacttc aaggaaaaac aagaatttat gctgcttgat tcttgttggt
    27541 gtctgatccg cacgaatcaa tgctaaaatc ggttttttga caatcgaaaa agcattgaca
    27601 acagcagcta ataaaaacaa aacagcaaaa aacaaaacag tgatcaacaa cccgtgaata
    27661 ctaaaaggtg taaaatgcga aatattgaga tctaactgat ccactaataa tcggtggacg
    27721 atacttgtca atcctacacc gacagctgaa cccactaaag tagctgcgat ccctactaag
    27781 aatgtttcag aaaaaatcat ctgtgcgatt tttctgcctt ttgctcccag catcataaac
    27841 attgcatagt ctttttgccg catcgtcatc aaaaaggaat ttgcatataa aatataaacg
    27901 aatgtgatga tccctagtaa aacagaaccg aatcggaaaa tgataactgt agccgcaacg
    27961 atcgtattac ttttcaaaaa agcttcgttg cttgccattg attcaaacat gtaaaaaata
    28021 gcagatgcca taactaatcc tgagaataac accaagtagt ctcttaaacg gctcttgatc
    28081 ccagtaagtg ataatttcca aagcatagtc aatctccccc tactgttcca agtttccaag
    28141 aatagatagt atttctcgat agaaggaatc acggctttga tctccacgtt tcacttcttg
    28201 atggataccg ccatccttga tgaataaaat acgttggcaa taacttgcag aaaacggatc
    28261 atgcgtcaca agaagaatcg atacatgatc ttttgtattc aattcgtcca tcgtatctaa
    28321 aagatcccgg gcacttttgg aatccagcgc acctgtaggc tcatccccta atagaatcgt
    28381 tggttgtgtg atcaatgcac gtgcggcggc gacccgttgt ttttgtccac cggaaatctc
    28441 agaaggatag ctttccaaaa tatgagagat ggataaacgt tctgcaattt tttggacacg
    28501 ttgcttgatt tcttttggtc tgacgccttg taaagaaaga ggaacagcga tattttcttg
    28561 tgctgtcaag ctttctaata aattgaaatc ttggaaaata aagccaatct cattcgcacg
    28621 aaaatctgct agttgatttc cttttaatgt ggtcacatct ttttgattga ttcggatatg
    28681 cccatctgtt ggtttatcta atgtggataa aatattcaaa agtgtcgact taccagaacc
    28741 agaagcgccc atgatcccaa taaattcgcc tttttctaca tcgaagctga tgccgcttaa
    28801 tgcttgcgtc tttttctcat tgttttttcc gtatactttt gttacattct gtacttcgac
    28861 tattttgttc atttctttcc ctacccttta attagttttt ccagtgctgt tttcggcacg
    28921 tctttttttt cgacttcttc atacgaagtg acacctttat ttttgttcca ggtgattttt
    28981 aaatatgctc ctttacgaag cggtcgagtc aattgtccct tgaagtccag ttctttttcc
    29041 ttaccctctt tcgtatagcc aggcaggata tatctatagt tgactgtttc ttgtccagag
    29101 tctgattttg aaactgtttt tgtaccatct gtcgtaattt gtacatagta ttcttctcca
    29161 cccataacta cgtaatctgc gattttcacc cctgcaagta ccacaacgat gaaaacaagt
    29221 agaccgatca gaagtttttt cactttttcc ccttctctct tactttatat actgattata
    29281 gaaaaaagcg aacaattctg ccttcgaatc aactatcatt cttctttcaa ttttgtcatt
    29341 ttctttaaaa caactaaaaa aagagttcaa gacaataatc cgtcctaaac tccttcttcc
    29401 tatcttacaa aaagtatgtc acttacggtc tttcatgtcg cctgtattga taacaggttg
    29461 cgttcctttg tctgtaatga ttgagacaag taaattattg atcaattgga ctttccgatc
    29521 atcggtgaaa ttgatttctt gtccatcttc gatttgctct aaggccattt gagtgattgt
    29581 gacagctcct tcaacaatgg tttgtcgagc agacaaaatg gcttttgctt gttgacgttg
    29641 gagcatggca ctagcgattt ctgttgcata ggctaaatgg ttcaagcgtg tttcgattac
    29701 ttcgacacct gctactgcta aacgctcttg cagctctttt tgcaactcgt cagacactgc
    29761 cgtcgtgttt cctcgaagcg tcaaatcatt ctcattgaat gtatcatacg ggtattggct
    29821 agcgatatgc cgaatggctg tctcactttg gatttctaca aaatcttgat aatacgctac
    29881 atcgaataat gcctttgctg tatcaacgac tttaaagact actactgctg agatttcgat
    29941 ggggtttcca tccaaatcat tgacttttaa aacagaacta ttgaaattcc gaactttcaa
    30001 cgaaacagtc attttttgtg ccaaaggtat cgtaaggaaa aagccatttt ctcgaatcgt
    30061 acctaaatat tgtccaaaaa acaaaattac ttttgcttga ttaggactga ctacagttgc
    30121 cgaacttaac aacaataacg tgatcaacca aagaaaaata cttaaaaaaa gtgcccaaac
    30181 tttcgcttgc cacataccta aatagaagaa aaacaatcca actaaagcta atacgaccaa
    30241 tccgacaatc cctgcataac cattgatata aaatgctctt ttttctctca tgctacttcc
    30301 tgcgctcctt tatccttcaa taaatatcta atgttacttt tttaattttt ttttcgaata
    30361 cgcgccatta caccttctag ttctaactct ttgtcttcag tgatgtacca accgcttgcc
    30421 tgtgtccctc cggctgcctg cggtaaataa tccaattttg ttcgaaccca gtgcttttct
    30481 tctgtaaaat agtcttctac caagacctct agatccatcc ctgctgaaag aggcaaatct
    30541 tcccgaacag atttaaagat aaatcgttcc ttcttcccat catacttcaa tattcctgta
    30601 tcgataatcg gtaaagaata ataatccagc tctttcttca ccgtcaataa atgtttgaaa
    30661 acttcatggt acacttgctc taattggcga ccttctgaat tgttcgggta attaaccatc
    30721 actttttctt tgtctggatc atagaaggtt tggcttagtt gagtgatata agtatgtaat
    30781 ctttccattt ctgtttttaa cttttctctt tgaccttctt ctaccttcat atactctttc
    30841 accctgtttt ctttttagat attttctttc ttattttact atagttatgt gcgttaagcg
    30901 atgaaaaaca gcaaagattg gaaggatctt tgctgttttt tcttaaaata tccagttgag
    30961 tacaggatca atctgtttca ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt
    31021 tagcagtgat ctttagcttt ttctgtcctt gttctaacaa agtatttgtt attggctggt
    31081 agtctttttc aatataagat ttaccagacg tttggatagt atagccactg ttttccatct
    31141 catgcaaaga taaattgcca ccgtttagga tgacttttcc ttctttgctg atcaaactgt
    31201 cttctagatg gaagttgctg ttttcacctg tgacgatcaa gttatccaca ttggcctcat
    31261 ctaatcgaag tgagcctaat tcatctttcg cagttattgt tcctcctgta aattcattga
    31321 acgataccca gccatcatgt gtcttagtat tcactttttt gattgatact tcgttgatat
    31381 caattgcact attattccct tcgattgtca attcagataa ttcatgatct gcagggacaa
    31441 caatagtgat ttgtgcactt gtgtcttcta aactaatgat tgcacctgtt gattttttga
    31501 ctgcattcac agtcaacacg ccatttttct cctttatttc tggcttttct gtcttatcat
    31561 aagtcgtttt tatctcaaaa tccaatccgc gtatgaccga aactgtttga ttttctgcat
    31621 ggatgacgat tttgtttgca tccttgattt tttttgtctc agtaaccata tcgatcaacc
    31681 gaggaccatt atcccaagtc agactctttt gagcgcctaa tgtataagtt gcacctgcta
    31741 ggaggccacc agtaagcatc agaccaatac cggtaatcaa aaaatgtttc aatctcaatt
    31801 tatgtgtcct ccttcaaacg cccgctgaat gcagatctct ttttgtaata aaacgtcgtc
    31861 ccatccattt aacaaagttc atcatgacat aaaaaagccc ttttgttcct ttcagcaaca
    31921 gtggcgaaat aaatactgcg cttcctgtgg cgaacagacc gacaccgata aagaataatc
    31981 cactcagaat cgattgtcca aacacactga ttcctgagat gatcatgaat aggccaacac
    32041 ctaagatact gatacataaa acatagatac taaaaatgag cgctagtaaa gcaaccaata
    32101 gtcccccaat aaccaatata aaccccagtg ctagtggaat gaggacagga gaagcaaata
    32161 aaccgataat gattaaccaa atcaggcgaa tttgtctttt tggatttttc actaactcgt
    32221 tttctgcttg ttcttcttct gaaaagtaac tgatttttaa ttttaaggca aactgctttg
    32281 gtgtaccata ttcatcaatg atctgatcta atgtttttcc agaatctaaa aaatattcct
    32341 cataatactg gatcaaatca aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa
    32401 cttcattcaa atattcattc atttgtgctc tcctccaata accggtctaa agaatctcga
    32461 aaaacaatcc attcatcaat aatttcttct aaatgacgac ttcccattgg tgtcaacgag
    32521 taatatcgac ggtttctgcc ttcaaaaggt tcatcatagg tctcaagata tccttctttt
    32581 ttcaatcttc tcaaaaccgg atatagtgta gattcactga cagagagact ttcttgtacg
    32641 atttttgtta aggcataccc atacatatct tccttgctta atgctgctag cacagtacca
    32701 tcaagaagaa cagttggaat ctggatgtgc attacgtcac cttctattct caagtgtaat
    32761 attttatttc aatctatatt atacaacata taatatagtt tcaaaatcag tttgtcaaat
    32821 acaaaaaaac tgactaaaag atttgatcct ttttagtcag caaaaaatgg cttctctttg
    32881 atttttatta taacagtggc ataatccaat ttgtccacat ccagctcagt ggcatgataa
    32941 cgatcattgc aggtatcatg tcagctactg ggaacatttt cacgttgatc attctaaaac
    33001 cggtagcgac catcaagaat ccgccgcaag ctttaaaatc taagatcatg tctggtgtag
    33061 tcaatggata aataaatttt gccaataaga acaaaatata aaaaataatg aattggggaa
    33121 ttgctatcac agaaacgaca taccctaaat tacacgcaaa aatagctgct gtgaaaaaat
    33181 caaggacaga tttagaaatc aaaatcgtac tgtctcctgt catcccagaa tctaaacttc
    33241 catagatccc cgtaccactt gcacaaaata gaacaatgac cgtcactaat gtgctgataa
    33301 attcatcgtg ggtcatacca agcttttcac tagggaagat tttagaaata ggtacttgca
    33361 tcagcgcagc ccccttattt atcccattac ctaaatgaac aatcaaacca atcgctgttc
    33421 ctatcactaa agcaaaaata acagccggca tatttttcat cggcgcaatg gaatagattc
    33481 ccatccccat cgaacacaca ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta
    33541 atttatttcc tactaatccg ccaattagtc cgccaaacaa aacggaacct acattgatca
    33601 aaactccagt tggcatcaaa aaactcctct ctttttctac gaagcgaaaa aaatcagaaa
    33661 aatttctgta attattgctt tcgctgcaag aaagattgta acagacagag aagattacac
    33721 caattcattt tttgtgttaa agtattcttt attggaataa gtaggaggaa tatgatggat
    33781 atccataaat tcgaagtttt tcttgattta gccgaaacaa tgaatttcac caaaactgct
    33841 gatcgacaat tcaccaccca aggaaatatt tctaaacaga ttctttcgtt agaaacagaa
    33901 ttagacgtca aattattcga acgcgccaat agaaaaatcg aattgaccga agctggaagc
    33961 ttattgctcc catatgtaaa aaatgtcgta gaacaatacc atgctatgca agaagtgctt
    34021 gaaacctata cgaaagacaa caatctttca ttaaatatct taacgatacc gacaatgatc
    34081 aattataaag gtttttccaa aatcaccgag tttttgaaac gccatcctga atttacagtc
    34141 cagttgaaag aagtggaaag tgtcgaatta acttacaatg acgccaatga acatagtgac
    34201 acgatccact ttgctagaag tttccaggcc ccaccaagtt cgattgagtg gttgcctact
    34261 gaaacagacg attttgtagc agttctacct aaaaatcatc ctttagctag taaaaagaaa
    34321 ttggatctaa gcgaactgaa aaaggagaac tttttgctcc ttggccccac tacgaacttg
    34381 tatcaacctg tactagatct gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa
    34441 ggagcaagaa tcgacttgat catcaatatg attgcaaatg acatggggat tgctattgtt
    34501 atggaaaaaa cagtcaagaa tctattaaaa gaaaatactg tgattctccc tatttctcca
    34561 acaacagaga gttatcttgc atttactcgt aaagtcggtg agcattcttt ggcttcagat
    34621 acattttggg agtatttaaa tcaagaagaa aactaagccc tacattttct gtccatctat
    34681 ccacccaaaa aaagagagcg acgcatgact ttcgctctct ttcttgcatc atgtccttgt
    34741 ttcaatgaca cctctaacat ttctcgtagt tcaataagaa agcagcgatt tttatgccaa
    34801 aagaaccgta agaacatttc cctaatcaca aaaaaacgac taaaaacccc taaaaacctt
    34861 caatagaatt tttaggggtt ttattctttt tcctattgtt tacttaaccg tatcattgct
    34921 gtcgttccta ctccttcgat actgctataa tcaatacgtc catcatgcag ttctagaatt
    34981 tttttaacca gtgaaagtcc tagcccattc ccagaaattt gacgacttgt atctccttga
    35041 taaaattttt caaacattcg atctctggtc tcttcattca ttcctacacc tgaatcggtc
    35101 acttccagta caatctctgt ggctgtctca aatagtttga tatgaatctg tccattgatt
    35161 tgattgtact ttatggcgtt atccatgata ttcagccaca cttggtacaa aaactcttca
    35221 ttgcctgtgt aatttactgc agcaagttcg atatccaatt ctagctgttc tttctcccat
    35281 ttaggctgaa aaaaaacgat tacttctctg atttgttcat ctaatcgata ctctttcttt
    35341 tccagttgga tacgctggtt ttccagtttg ttcaatttta agatgttctc tgttaattgt
    35401 gaaagctgcg tgatactttc gataatccga tataaaaata tttggcgttc ttcgtcagag
    35461 agatttgggg cttgtaacaa ttgaacatat ccttggattg ttgctagtgg tgttttgaat
    35521 tcatgggata cactggacac aaagtcattc ctcagtgtct cgatactatt gagttcttgg
    35581 accataacgt tgaaatcttt atacagttgc tgtacttctg ctactttctg ctgttcatcc
    35641 attcgaatcg aaaaatctcc cgttgctacc agactcatat ttgtccctag cgcactgatt
    35701 ggatgcagga tacgccgccc aacaaaaatc gatattcctg tacccacgac catactgaac
    35761 cccgcaaaga cgaataacgg gtataacgtt ggttttccag ccgattcagc aggcgagata
    35821 gaatgtcgag acaataagaa aataaaaccg agaaaacaca gcagtgtcac taagatcgaa
    35881 atgaagacaa tactagcaaa atagatccat aattgtgaaa atagcttttt tttcatcttt
    35941 aaataaccgc cttatagcct agtccccgta ctgtgatgat ttgaaaatct tccgtctttt
    36001 caaaacgggt ccttaaacgt ttgatatgta catcgatcgt tcgttcttct gtattcgtat
    36061 ctaatcccca aatatcatcc atcaactgtt ggcgagtgaa gatcttgttg ggataagaca
    36121 gtaatttata cagaagcaga aattcttttt gaggcagaat caacgaatgc tgatcttgta
    36181 acactgtata agcatctagc tctaattttg tttgtccgat tatcaagcta ctgctgtaat
    36241 tgatctttga acgtcgaagc aatgcttcca cacgtaggat catctcattg acatcgatgg
    36301 gttttgtcat ataatcatct gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg
    36361 cctttgccgt aatcattaga acaggtgtgt caaatctggt gctacgtaat gagtctagca
    36421 agtcatagcc atccattttc ggcatcatga tatctgtaat gattaattct atacgtgtcg
    36481 cttctagttt tttcagcgca tcctctccat tcaatgcggt gatgactgaa aagcccgctc
    36541 gttccaacac agaacggtaa agcaactgta attgcggatc atcttctact actaaaatac
    36601 gattcattga tctccctctg ttttttgaaa aatttctaga ttttttcttg tagttcatct
    36661 tcagttaaca tttatttcgt atactacgat cattgttctg atatacttat tagtataccg
    36721 aatatttcaa tacgggagaa gggtgattat gcttcaatta aaagatataa aaaaatatta
    36781 taaagtcggc gaaaccacca ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa
    36841 agaattcgta gcgattttag gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat
    36901 tggtggacta gataattatg attccggtga tatggtaatc aacggaaagt ctactaaaga
    36961 ctttaaggac agtgactggg atgcctaccg aaacaactct atcggtttca tttttcaaag
    37021 ttacaacttg attggtcatc taggcattat tgaaaacgtt gaacttggga tgacattaag
    37081 tggtgtttct aaagacgaaa aacgaaaacg ggcagaagat gcattgcacc gagtcggctt
    37141 gacaaatcat atgcataaaa agccaaatca gttatctggt ggacaaatgc agcgagtggc
    37201 catcgctcga gcactagcca atgacccaga tatccttttg tgcgatgaac caaccggagc
    37261 gttagataca gaaacaagta tccagatcat ggaattgatc caagagttat cgaaagaaaa
    37321 attagtgatc atggtcacac ataatcctga actggcccat caatacgcag atcgaatcat
    37381 tgaattttct gatggtaaga tcttaacaga ttcccatcct cacatcgaac gaccaaaaga
    37441 tgatcaattt aacttacgca gaacaaagat gagtttttgg acagcgttaa agctttcctt
    37501 caacaatatc cgaacaaaaa aaggacgtac tttcttgacc tcctttgctt ccagtattgg
    37561 gatcatcgga atcgcgatcg ttctgtccct ttcttctgga ttccaaaaac aaatcgataa
    37621 cacgcaagct gaaacgatgg cgaaatttcc gattactatt tctaaggtaa cgactaacca
    37681 gactcgtgac gatgccggat tgggtgcaag caaagcggat tatccagaca gcaaaacgat
    37741 tactgcaaaa gtcagcgaag aagataaagc acagcacacg aataagattg atcaaacata
    37801 cgtcgattac gtaacagaca tcgatcctaa cttaagtaat aatatcggat ttacccgtac
    37861 tacaggaatc aatcttttac gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa
    37921 tcaaaatcct gatacggaat ctctttcttt atctagtacg atgtcagcaa tgacaggtgt
    37981 gggagtgtca agtttcccta cacagcttga cactagcaaa gaaaactttt taaaagacaa
    38041 ttattcttta cttgccggaa gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga
    38101 cggcaataat aatactaata tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga
    38161 cgaaaaactt gattttgatg atatcgttgg tacaacattc aaattagtaa acaacaatac
    38221 ttactataca aaacttccaa ctgggaattt tatccctaat acagattatg atgcgatgta
    38281 tcagaatgct tctgatgaat tgaagatctc ggggatccta cgcgtgaaat cttcttctac
    38341 aatgaatctc ttatctccag gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa
    38401 cgaaaacaaa gaatcagaaa tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac
    38461 tactgaaaaa gttgacgaaa atgccaaaca aacattgctt agttatttag gtggagacag
    38521 cttgccttca agtatcatga tttatccgaa caattttgaa gacaaagaaa agatcttgga
    38581 ttatctagat gattacaata aaggcaaatc agatgaagat aaaatcattt atactgattt
    38641 agctggaacg atgactgaac tgactggcgg attgatggat gctatcactt atgtccttat
    38701 tgcttttgca gggatctctt tagtaacaag tatgatcatg atcagtatca ttacttatac
    38761 atctgtgatt gaacggacaa aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa
    38821 agacatcact cgcgtatttg acgcagaaac gtgtatttta ggtatttctt ctgggatatt
    38881 aggtgtgttt attgcatggc tagctacgtt ccctatcaat agcatcttat acaacatgac
    38941 ggatctaaaa aatgttgctc aactaaatcc tgtccatgcg attattctag tgatcgtctc
    39001 tactatattg acgatgcttg gcggtcatct tcctgcacga atggcagcga aaaaagacgc
    39061 cgctatcgct ttgcgtgcag aataaggttg tgacaaccac atttagattt gagtcataag
    39121 agcgctgaaa tagtagaaaa cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc
    39181 tgtttttttt aattattttt tataattctg gtatactttt ctttcctttg taccacaact
    39241 cttctggcgt atacctgtct tttccattta tgaatactgt tccatctgct tcaatcgtta
    39301 cttcagtccc aatccacata ttacgagaaa gtgcgagaag attttttctt tttcctcttc
    39361 ctttttttcc aaattgcaag gcatcggtta tctcttctgt ccaatgagat ttcacgtatt
    39421 ttacttctcc gttttcaaat gtaactttca aaatattcgc tatattttcc acttttactg
    39481 ctttaggaaa tgactcctcc atttttttca ctttccttcc tccaacacta agtatgcgct
    39541 ttcttttagt gataagtata tcatagttta ttctattatc aaaaaaacgc atccattcca
    39601 ttgtagaata ctttaccgaa caagttgaat tgttttcctc tttttcacct tttactatag
    39661 ataggtaaag acaactaagg aggaatctct catgacagat caaccgtatg atttaagaaa
    39721 agtcatcgaa gaactaaaaa cacttccagg tcaatatcac gaaacagata ttgaaatcga
    39781 tcctgaagca gaattatctg gtgtttaccg ttatatcggt gctggcggta cagtaaaacg
    39841 tccgactcaa gaaggaccaa ctatgacttt caacaatatc aaaggtttcc ccggcacacg
    39901 tgtgaacatc ggaacaatgg ctagtcgtaa acgtgtaggt gaaattttgc accatgatta
    39961 tcgaacactt ggtcgtttct tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt
    40021 agacaaagca gacgctccag cacaagaagt cgttcatcta gctaccgatg cagacttcga
    40081 tattcgcaac attttgcctg cacctacaaa tactcctgaa gatgctggtc catacatcac
    40141 aatgggtgta gtcttaggat cagatccaga aaaaacaatg agcgatgtaa cgatccatcg
    40201 tatggttcta gaagataaag atacgatcgg tatgtacatc atgcctggcg gacgccacat
    40261 tggtcacttc caaaaacaat tcgaagcctt gaataaacca atgcctatca ctatcaatat
    40321 tggtttagat ccagcaattg ccattggcac gactttcgaa ccacctacca ctccacttgg
    40381 ctacaatgaa ctatggtgtg caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg
    40441 tatagcagtc gatgaagtgg gtattgctcg tgctgaattc atcattgaag cagaaatcat
    40501 gccaaatgaa acaatgcaag aagatatcaa cacgaactca ggaaaagcaa tgccagaatt
    40561 tcctggctat actggggatg ctaatccagc tgtacaagta gtgaaagtca aagcagttac
    40621 tcaccgaaaa gacaatccaa tcatgcaaac aacgattggt cccagtgaag aacacgtatc
    40681 tatggccggt attcctacag aagcaagtat tcttgccttg gtcgataaag ctattccagg
    40741 aaaagtagta aatgtctaca atcctcctgc aggtggtggt aaattgatga ccatcatgca
    40801 aatccgtaaa gaaaacgaag cggatgaagg tattcagcgt caagcagcta ttcttgcttt
    40861 atcagccttc aaagaattga agacagtcat tttagtcgat gaagatgttg atattttcga
    40921 catgaacgat gtagtttgga ctttcaacac tcgtttccaa ggagataaag atatcatcgt
    40981 gatcccagga atgaggaacc atccattgga cccatcagaa ttaccagaat ataatcctgg
    41041 ctctattcgt ttccgtggga taagtgcaaa aactatcttt gacggtacag ttcctttcga
    41101 tatgaaagaa cattttgtac gtgcaaaatt ccaagaagta gaagattggg aaaaatattt
    41161 gaaataatga atgatctgca actgttttga catcataaaa aggagtgcgg aaaatggaac
    41221 aattggcaca acggaagaaa aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt
    41281 atgccatcaa cctagtgaag aaactaaaag aatatccgat gatagaaaca cacggaatca
    41341 tcagtccatg ggcaaaacag aacttaaagc tggaatccga cttatcacta actgaattcg
    41401 aaagcctgtt tgattaccat tattcaaaca aagatttagg tgctgctgtc gccagcggat
    41461 catttttgac cgatggaatg atcatcgtac cggcaagtat gaaaacagtc gcaggtatct
    41521 ctgttggttt aggcgacaac ctgattgcac gtgctgctga tgtaacaatg aaggagcaac
    41581 gaaagttagt cattgttcct cgtgaaacgc cattaaatgc cattcacttg gaaaacttga
    41641 ctaaattagc acgactaggt gtgcaaatca taccaccaat tcctgctttt tataatcagc
    41701 ctcaaacgat ccaagatctt gttaatcacc atacaatgaa acttttggat caatttgaaa
    41761 ttgtaaatga gcattcaaaa cgttggcagg gtgattaata tggaaaaaga aacgatagtt
    41821 gaagtaacca aactaggttt cacgatccaa gctgctgtta aagtaatcgg taaagactta
    41881 ttgatcactt taacaggcgg agatacacca catatcggaa cagtcactac attttctcgt
    41941 gatacagaga tgcaaactgt acgtttccct agccacgatg gtcggttcca taaagacgat
    42001 gtcttagccg aaaaaatagc tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc
    42061 acttccggta tccatgttga ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg
    42121 tcagaagagt taggttacaa gattttagat tggttggaaa aaatagattt ccatatcaaa
    42181 gaaccagttt attataaaga tggtgaaaaa ccattatgaa agaagaggtt gtgaaatcag
    42241 cgttatgacc tgagtatgag agaaaaaaat ggaaaaatag cttctcatat tttgccgttt
    42301 ttgagctaat accgagggac agctgattga acgccgttta tcaagaggtt gtgataaagc
    42361 cgcttcgttt cgagtagtaa gaaaaatttt gtaaacatgg gtttccacat ttttgcataa
    42421 atttgactta ctaccgagaa actggctttt gaacaccgtt tatcaagagg ttgtgacacg
    42481 tcttttgtca caacctcttt tatttttgct tcactgtaac tgctacaata tcaccgattt
    42541 cttttccgat gtttttacga atatcttttc gtagtccaag gatgtagcag actgaaccat
    42601 ctggattttt cacgcccata ttcacgatac tgccttcata tggcacattg tcaaaagttg
    42661 cttggacttt aactcttcca gcaccgaatt cttttcttat atcatacgga aaaataatat
    42721 atgccccgcc cttgccgact tcggaagcat agatttttga ttcaaaagaa taaatttccc
    42781 tcattttatt tctcctattt tttacatcta gtatagcata gcaaaaaaaa gaaggaactg
    42841 tgacaaaagt cttgtcgcag ttcctttttt cgaataaacg gtgttctcct gccaactcct
    42901 cggtcacaag tcacaatagt caacaaacat gagaagctgt tttttgaaat tgctccttgt
    42961 gactcagagt taaacggcag gctcacaacc tcttccttaa acggtgttca aaatattatt
    43021 cgccttttgc ttctttcact aattttgcga atgcttgttg ttggaattca gcagctaagg
    43081 ctttcgtacg ttgttcttgc tctgctttgc tcatttttgg attttcagtc attgctttta
    43141 ccataccgtc ttgaacataa gcttttgctt cactttccat cgtactttgc tgtttcttca
    43201 ctaattcagc taacttggct gcttggtccg tacttaattc tacccatgtc tctgggattt
    43261 tgaatgtatt cactcggctg tcatattcgt gattgttatc agcaatattg aaccagaatt
    43321 tatacatgtc gtttttgtat tcccaataag tagttttcgt cactttttca gcatcgccgt
    43381 ctgtcttttc tactttattt gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt
    43441 tttcatttgt tcggtaaaga tacacttttt cttttccatt ccctaaagct ttatacaaaa
    43501 gcatatctgc tccttgggaa tcagcactgg aaacaatttt ttctgttttt gtttccgtta
    43561 ctttttccat tccaaagtga ttggacaaat tagcgacgat caaacttagt gaagcgacga
    43621 agattagtcc gaaaatcaaa gataaaaagg tttgccatgt ttttttagcg aacacgttcg
    43681 ttaaagcaaa agcaagcaag ctgacaatca aaactccaat aatcatgcat tatcccccgc
    43741 tttcattaga acaggttctt ttttcttcaa taaaaaggca ataataaagc caagaatccc
    43801 aaagatgatc gctacgataa acgcggcatg gtaacctgac agtgtggcat tgattgcctg
    43861 atctttataa gctaatggcg tattatccaa tacagatttg cttgggatat ttgcttttgt
    43921 cacattcgtc aacacactga tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa
    43981 tgtattattt accgctgtcc catgactgat caagttattt ggcaatgcat tcataccaga
    44041 agtcgttacc ggcatcatga ccatggagat cccaaacata cggatcgcat aaaggatcac
    44101 gatatataaa actggagtcg tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa
    44161 aatgaacatc cctgtgatgg ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt
    44221 cacaggcatc atagccccca tgatcaatgc acccggcaac aacatcaagc cagaatggaa
    44281 tgctgattct ccacgaatat tttgaatata caatggtacg accatttctg cccctaccat
    44341 cgccatattt gttacaccag ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac
    44401 acgaagttcc aagaatggat gttccaaatg taattgacgc catgagaaca aggcgatgaa
    44461 aatcacacca acaactaaga aaccaattac tttcaagctt ccccagccgt catctcctac
    44521 actagaaaat ccgtaaagta aactaccgaa tccaatcgtt gataaaatag cggataaaat
    44581 atcaatgcta ggatttgaca atggaataac acttttcatt aggaagaatg ctaaaaccaa
    44641 gacaagaaca acgattggca gtaccatacc aaacaagtca cgccaatggt agttatcaat
    44701 gatataacca gataatgtag ggcctaaagc aggagcaagt ccaataacga tccctgacat
    44761 ccccattgct gatcctcttt tttctggcgg gaaaatagat agcatgatat tttgcaataa
    44821 tggcatcgat actcccacac cgcaagcttg gatcaaacga ccgattagta atgtactaaa
    44881 attaggtgct gtaaagcaag taatcgttcc gatcaaaaag atcgtcatgg cagaaagata
    44941 taaatttctt gaactgaatt tattgatcag ccatgctgtt attgggatca tgatcccgtt
    45001 gactagcaag aaaccagtcg ttaaccattg cacatcagac gctgaaatat cgaaagcttt
    45061 catcaatgta ggaaaagctg tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc
    45121 aattaataag acaacaacta aaagtgtccg attatatggt tttccgtaaa tatctacggg
    45181 ttggttgtta ctcatatttc tccttctttc tgaatttttt ctgaaactac tagaatactt
    45241 tagtcctaaa ttcgctattt gtcaacctac ttgacacata agttaatacg gatataatta
    45301 agggaagcaa gaggacaaaa ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt
    45361 tggaaaatga tcaattggtt gttggcatca gtgaattaag tgatatcgta ggtgtgtctc
    45421 cacgtcaatt aaggtattgg gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga
    45481 attgtcctag aaaatatcgt ttacctacag tcataaaagt agaactgatc aaaaaatatt
    45541 tagatgacgg atttactttg tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa
    45601 tgcaccatgt ccgtaaagtt ttttcaaaag caatcaaaga tatcgaattg atcgacgaac
    45661 gctatacgat tttacaaatc ggtaacttca aagaagagaa tgctaagatg tacatcattc
    45721 atgacaacga gacagacgaa ttaacttaca agctccttcc tgtcgatgaa aaagctgact
    45781 acgaacagtt gttaaaagaa ctctaaacgc cactaatctc ctaagtacca agctttttaa
    45841 taagtagatg ctacataaat acttgaaaaa aggcctgttt gaacataaga atatccgaac
    45901 ggtgatctgc gtttctttca tatagaaagg aattctccat cactgttcgg atattttttt
    45961 atttttttcg gttactttct tctaatacat ctgttagcca atttgcatat tctgtttccc
    46021 gattgatcgc atgcgctaaa atcaggtagt gtccgtaatt ttcatgtttt tctttttcag
    46081 taggaaagat cgtttttttt cgctctaata gatgaagtcg tttttcttca tgaaggtcac
    46141 gctgctgctg aataatttct actaatgccg gatcattcac gtctttgacg aaatacaatt
    46201 tcagaacaaa ttcgtctcga ttaaccggta gttcattggt gggtgtatga atccactctg
    46261 ccaattgttc cttcccttct tctgttaatt cataaacttt cttttccaat ttttcacctg
    46321 tgatctctac ttgatgctga atgattcctt gctcttccat ttttgcaagt tcaggataaa
    46381 tctgactatg ttttgcttgc caaaattctc ctatttcatt tttgaacgat tgattcaatt
    46441 catatcctgt catcttcttc ttacttaata accctaacaa aatatatcga agtgtatttt
    46501 ttcttgccat cagttttccc tccttgacaa atctttaaat ccattctatc ataaatgagt
    46561 aatcgaaaac atgtagttta ttacatgttt taatttataa ggagatgctt aaacatgaaa
    46621 gaatttaaag aattggaaga ttttattgga actcacttta tctatacgta tgacaatggt
    46681 tgggaatatg aatggtatgc caaaaatgac catactgttg attaccgtat ccatggcgga
    46741 atggtcaaag gccgttgggt aaaagaccaa gaagctgata tcgttaaatt gacggacggc
    46801 gtattcaaaa tcacttggac agagccaaca gggacagacg tggcattgga ctttatgcca
    46861 aatgaaaaga aacttcacgg aatgatcttt ttccctaaat gggttgaaga acatccagaa
    46921 atcactgtta ctttccaaaa cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat
    46981 gaaacttatc caaaattact tgttcctgaa tttgctacga tcacttatat gggtgatgca
    47041 ggtatagaca atgaagatgt tattagcgaa gaaccatacg atggaatgcc agatgatatc
    47101 cgtagcggaa aatacttcga tgaaaattat aaacgagtaa aataaggttg tgaaagagag
    47161 gttgtgaaat cagcgttctg acctgagtat tagagaaaga aacggaaaaa tagttcctca
    47221 tgttttgccg ttttttgagc taataccgag ggtcagctga ttgaacaccg tttatcaaga
    47281 ggttgtgaaa tcagcgttat gctttgagta ataagaacgc tagttagaaa atagcttttc
    47341 atattttcta ggctagtggg cttattaccg agaagcagcc gtttgaacac cgtttatcaa
    47401 gaggttgtga aatcagcgtt ttgacctgag tattagagaa acaaatggaa aaatagttcc
    47461 tcatattttg ccgttttttg agctaatacc gagggtcagc tgattgaaca ccgtttatca
    47521 aagattgtaa caactgcata ttcacacagt taaattagtg acttcaaaat gaaataatgc
    47581 tcccagcaaa aaattaggct gggagcatta tttcgttatt ctgttcatca aaaatcagcg
    47641 ttttgattcc atttcatcac aagttcctcc tcatttactg cttcatcttt ttgctgtgtt
    47701 tgaattgtaa aaccttgtga aagatagaaa tgaatagctg attgattttt ttgatagact
    47761 gtcaaagtca gtgttttatt ggtttctttt atttttttca ttagttgcgt accgattctt
    47821 tgttgccggt attccctttt cacaaagatt cctgcgataa actgatcaat caccccagca
    47881 aaaccaataa tttcttgatc tatttctgct aagtaaatca ctgcatctgg taaagctttc
    47941 ctaacagatt ctacttgatt ctcccaataa gttgatggaa tgaattcatg agaatccaag
    48001 ttagcatgca gccaaatcga tagaacagat tccagttctt tggcagacaa ttttgttgtt
    48061 gtgcggatca ttttttctct ccttacttat tcattgattt taaaataaaa accatttttt
    48121 tgaagctctt ttacaagttt tcgcccctca aaaaagtata tcttctcttg ttttactttt
    48181 ctaataccaa aagaaacagc aactttagga tcatcagctg aaacgattcg ataagttcca
    48241 ccaggcaatt gatccagctt gactttccca tgatggtcgc tttgtactga aaacggcaca
    48301 ccatttctag taagtgtttt tttcttatgt ttcaaaatga atgaactacc atttaccggt
    48361 ttccctgacg aatcactaat aataaatgaa aaatcaagca cttttttact taatctatac
    48421 tgatagatta gtcgtccgat aaacactaac agtatgacta cagctattat cgttcctgca
    48481 agaataaacc attgccgcat ggttctttcc tgatttcctt ttccttcttc tttttttacc
    48541 gattcgttat aaggaatccg atgtcctgtc actagcaaac gatgcgtgtt gatcatatac
    48601 ggcgtacatg taagcaaggt aactaagtct tctccttctg aaatcgttag aacagacgtg
    48661 tcatctggca gtaccacttg tatattttta acttgataag ccaatttctc ccccaaaaca
    48721 gtcaaaataa acgtgtctcc gttttctaaa cgatctaatt cggtaaataa ccgtcgatta
    48781 ggtaaaccgc tgtgagcaga gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag
    48841 gttccttgca agacgcctgc tccattttca aggatcctac cattgagccg atcataaagt
    48901 gaaatattta attggatctt aggtatattg atacttccga tcagccattc ctctaattgg
    48961 ctggatgttg tactttcatt tctgttcttt tcattgaaag gatcagtacc aggattgaat
    49021 ccttggtcag ttaatcgttg gtttttctcc tccattttct ttcgctgcac ttcaatttca
    49081 gctgcggtcc gtttttgtac ttgttcgata cgtttttgat caataaaact atttaaagaa
    49141 tccacataaa atggatataa agcgatcagt aaccctacga cgatacatat taataaaaac
    49201 aaacgaaaca aaagtcgacg agactgtttt tgcttactct gcttcacatg tgtcctccct
    49261 tgcaagtttt tttgttcttt cttttattat atgggttcag gaaaaaaata gaaaatgaaa
    49321 gccgatgagg ttgtgaaaaa gctgtcctgc atcaagtaat aagtacagcc caaaaaaata
    49381 gctactcata ttttttgttg ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    49441 tttaaggaag aggttgtgaa aactgcgtta tgctttgagt aataagagcg ctagtcagaa
    49501 aatagcttct catattttct aggctagtga gcttattacc gagaagcagc tgattgaaca
    49561 ccgtttaccg agaggtcatg aaaaagctac tttgtttcga gtattaagaa aaattctgtt
    49621 tcaaaacaag gagatgcaac aaaacttatg tcgcatcctc tctccttaga actttcactt
    49681 atgtttttct tgtaaaattc ataaatattt tcatgaattt tctaatttct tgcctacctc
    49741 tcgcatagga tatatgatga atttatctaa agtttgcaaa ggagttcgac aattgaaaaa
    49801 atatggccca tttttagcaa tttttttcat tgctttggca tttctcatgc ctcatttatt
    49861 cacacataaa ctaattttgg gtgctgatgc cccattccat tacaatcggt tctatgatgc
    49921 cgcgcaacaa attaaatatg gggatttcca ttattttcct tccctttatg gattccagca
    49981 aagtggcagg atcgtgaatg ccgtttacgg cccatttttc gcttatttac aaggattgat
    50041 cgtgttgctt gcaggtagct ggtttcatta tcagattctt tcaaatttca ttctttatct
    50101 tatttcagga ttttctatgc tgattttact gagatatgtc aatgttcgaa attgggtaag
    50161 tgtaccagta gccgttttat tcatgagtac gtattctatt caatactggt ctatcaacca
    50221 aggattcact agctggggga cttgttttta tcctttatgt atgatcccta ttgctgattt
    50281 cgtccttcat aaaaagttcc ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca
    50341 gatccattta ctgtcttcga tcatgttagc tttgatgtat atcccttttt atctttatta
    50401 cttttttaac cagactgaaa aaggaaaagc actgcttcaa ctgattaagt cggtcttgct
    50461 ttattttgtt ctgacagcaa atatttgggc aggattagct attgtttaca gtgggaataa
    50521 gattcttcct ccctttgtta acagagaaat gagtcaaaag gcgatagatc tggatggcag
    50581 ttattggctc cattacccta aggcctttcc tatattgata ttatcaggtt ttttgctttt
    50641 tattttcttt caacaaaaga acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt
    50701 tttctttttt ctttctacta gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc
    50761 ttttattagt ttgatccagt ttccatttcg atttttcgtg ccatttacgc tattgcttct
    50821 actttacctt gccctttcac tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag
    50881 tatatttggc attcttattt tttcagcaca gacgatccag aacttatacc agcatctgga
    50941 aaagtgggaa aatgaaacat ttgtcagtcg acatacttat ctattcgata cgccggaaga
    51001 agcaaggaaa acattcttca gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac
    51061 cacacctgac tatctgccaa tctataagga gacaaaagac aataaatacg accggtataa
    51121 cgaagaaata ctagaaaacg aaacaacttt tataaaaaca catccaggcg gtcttttgac
    51181 aatcaagtgg aaaaacactg gggacaaaac tgttcatatc cctgttatcg tctacgatcg
    51241 gactgtgcta cagcaaaatg gaaaaacatt gactgattat gaagtaacag atatcgggac
    51301 cccaatcgtg aagcagcaaa aaggaatcaa cgagctcacg cttcattatc aaacgccaat
    51361 ctatttctat ttcattcttt ctcttacttt gattggctgg tttaccttgc tatgtttatt
    51421 tatttatcat agatacaagc ttttacgtgc ttaggcaaaa gggataaata catccgatca
    51481 aaaaactctc caaacggtat aatcgtttgg agagttttat tttaatcagt ttttttatgt
    51541 tatatgttat attttttatt cgttcacatc taattcgatt tccaccccgt agtgatcact
    51601 gatgattgga ctatcgatcc catcaaaaat ccgttgatat tttttaacct gcattccttc
    51661 agggacgaaa ataaaatcta tacgcaattt ttctgtattt tgttcccaac catctatttt
    51721 cttttccaca gtagctgctt ttcctttttc attcgcgaca acatacgaat cttgaagaag
    51781 aaggcaactt tctcttacag tataataacc ttgagagtct actggattat tgaaatcacc
    51841 taggaaaaaa agtggtacgc gagtttccaa aaagtagttt tccaagtttt tccactcaaa
    51901 ataaaagcca ctttttttat ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc
    51961 ctgatcatct atctttgttt tcccaatcaa gatcttgcgt gtctgataat cttctttctt
    52021 ttgaaattgg gagacggtaa ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt
    52081 tccttcttca tatttatcat aaccgatgtg gctcatttcc caactccaaa aatactcttt
    52141 tccgtgttcc tttaaaagct gaactaaacg gtaggcaaaa ttatctttac ggataggtac
    52201 ttgattcttt accggacaga acttcattag cttcgcaggt tctacttctt cagattccag
    52261 taattggttg atttcttgta aagcaatcac ttcataatct tctaacagga tcttatctgc
    52321 taattcttgc aattttttct ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat
    52381 gaatttcatt ggttttctcc ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt
    52441 tgtcacaacc tctccagcaa atagtgttct cctgctaact cctcagtaat aagctcaatg
    52501 gaccaggagc tattttttgt ttggctgttt ttatcacttg gtgcagggca gctttttcac
    52561 gacctctcca ctaaacagta ttcaaactgc tgcttctcgg taataagccc aaccaaacga
    52621 aaaataggag gagctatttt tgtttggctg ttcttatgac ttgatgcagg acagcttttt
    52681 cacaacctct ccttacattt taatggagcc gatcacttta ccagctgtca ctgtacctaa
    52741 ttgcttgatc actacttctt ctactttatc gtttgtaaat acgatcatga tcgttggatc
    52801 tttttcttcg ttttggatca catcaagatc catctcagct aatttcgttc ctgttcctac
    52861 ttcttggcct tctttgacag caatctcaaa agctggctga ttcatttgga ctgtatcgat
    52921 tcccatatgg atcaaaactt cgataccatc tttcgtttcc agcccaattg catgtttgct
    52981 agggaaaata cttgtcactt ttccagcgat cggcgcaaca acttctcttg atgttggaat
    53041 aacagcaaag ccgtcaccca tcattttttg tgagaatacc ggatctttta cttcagataa
    53101 aggaatcact tctccagaag ctgcggaaac tagttcttgt tctataccta agtattgctt
    53161 actacctgct tgtccatttc ctgtagaaac catttctggc tctggaatct ctgctcctga
    53221 ttgcagcaga tcttcgatat ctgatttcaa gacatctgct tttggtccat aaactgcttg
    53281 gactccattg tctttgacga tcaatcccat cgcccctgct cgtttccagg cttcttcagt
    53341 ccctactttt tctcgatcat ttacacttac gcgtaaccga gtcatacaag catcgacatc
    53401 tttgatattt tcttttcctc ccagcaaatg aatgatctgg accacttgct gatctgctgt
    53461 tcctgttccg ctgttagata caacggtttc ttcactgtca ttgtcgtaat ttccgttacg
    53521 tcccggtgta gcaaagttga attttttgat caagaaattt gctaagaaat acgtaacaac
    53581 accaaaagcg atcacacaca acacgaagtt gataagatct ccacctagac ccgctttgat
    53641 agctaacggt gtccgagtca gcaattcgat attcccaaat gaatggaccc gcaatggcaa
    53701 gatatctgcc atcgcaaatg cagccccttg gatcactgaa taaatgacat ataatggcac
    53761 agcagcaaac ataaacatga actccaatgg ttctgttaca cctgttagaa agacagccaa
    53821 cgctgctgaa aaatacattg atttatatct tgctttctta tcaggatcga cattacgata
    53881 catcgcaaaa gccattccca tcaagatacc tgaagaacca atcatttgtc ctactttgaa
    53941 acgagccggc gtccagttat gcaacacaaa ctcatattgc gacatatccc ctgcaccttt
    54001 aagattcact aaatcagtgg cccaagccaa ccataacgga tcttgcccaa atacttgtgt
    54061 ccccgcctgt gcgcctgata aaatctcata cgatccgcca agctgtgtat agttgatagg
    54121 aatcgtcagc atatgatgca atccaaatgg caatagtaaa cgttctaatg ttccaaataa
    54181 aaacggtgct agaatcggtg cgctttcttg tgattctgca atccataaac caaaattatt
    54241 gattccagcc tgtacgtttg gccacaccac agcaagaatc aatgccacga ttgttgacca
    54301 taagataaca acaaacggaa caaagcgctt tccattgaaa aatgacaaag catccggcaa
    54361 ttttctataa ttatagtact tattataagc cattgccccg acaaatccag cgatgatccc
    54421 tacgaatact cccatattca aagctggtgc ttccaataca cttgtaaaaa atcctttaac
    54481 catgatcttc gtaccgaaca gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact
    54541 tgtgacacca aagatagccc cagtgatccg attgattaaa ataaatgaga tcccagctgc
    54601 aaatgctccg cctgctcttt cttttgccca acttcctcct attgctaaag caaataaaat
    54661 atgaagatta ccaataattg cccaaccaat attttcaatt actccgcccg ttgtgacaag
    54721 cggcgtccaa tcaggattga tcaagggaat tgatttccct atactgatca tcaacccagc
    54781 tgccggcata actgctacca caaccattaa agcctttcca aatttttgcc aaaactcaaa
    54841 actaaatatc tttttcattc cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt
    54901 atagcgtgcg ttttcttttt tgtaaatgct ttatttttta tttttctgtc tataccattc
    54961 tataaaacct ataatacgtg tgttcccttc tataattttc tcctaaaaat ttattttttt
    55021 aagctttttt ctatgaaata ttttcttttg tctattgact tgtgcaatcg gttgcgttta
    55081 ttatgaacgt ataaaacagc tcattaggag gagaaaaatg aaacagatca aacgactatt
    55141 tgctattgat ccatggaaaa tcagcacgac acaattagat aaaaaaaatc ttcgactgca
    55201 agaatcttta acaagtattg gaaacggtta tatggggatg cgcgggaact ttgaagaaac
    55261 ttactcaggg gaccaccatc aaggtactta tttggcgggt gtctggtatc ccgataagac
    55321 acgtgtcggc tggtggaaaa atggttatcc agaatacttt ggaaaagtga tcaacgcctt
    55381 gaactttatc gcgatagata tttcgatcaa cgatcagctg atcgacttgg cttctttgga
    55441 accagaagac ttttactggg aattagatat gaaaaatggt gtattatctc gtacattcac
    55501 aatcactact tctactaata aagtacgcat ttccttcgaa cgttttctga gcattgtcaa
    55561 aaaagaatca gcttatatct atttaactat agaaatgctt gaaggtagcg gaacagtcaa
    55621 actcgtatca aaattggatg gaaatgttca aaacgaagac agcaactatg atgaacattt
    55681 ttggaatgag attgatcgtg gaactataga agaaatcggc tatttgacga ctaaaactat
    55741 tccaaacaac tttgggattg aacaatttac tgttactgca gcaatgcgcc atttcgttaa
    55801 tggaaaatca gtcatccctg actatcacga agatacttta gcattacatg gttgcttgtc
    55861 tgcagaatta tctcaaggtg agacatggtc agtggaaaaa gaaattatcg ttcttactag
    55921 tagagatatt ccagaagcgc agcaagcaga aaaagctgtc tcagaattaa cgaaccagac
    55981 aaacaattat atccaagcaa aagaagagca aacagcggct tgggcagaac gatggaagct
    56041 tgccgatgtg gtcatcgaag gagacgaaga agcacaacaa ggtattcgat tcaatttgtt
    56101 ccaactattc tctacttact acggcgaaga tgaacgattg aatatcggcc ccaaaggatt
    56161 cacaggagaa aaatacggcg gagccactta ctgggataca gaagcgtatg ctgtccctct
    56221 ttatcttgct ttagccaaac ctgaagtaac taagaatctg ctgaaatatc ggcataatca
    56281 actgccgcaa gcaatccaca acgctcagca acaaggatta aaaggcgcgc tttatcctat
    56341 ggtcaccttc actggtgtcg aatgccataa cgagtgggaa atcacattcg aggagattca
    56401 ccgaaatggc gcgattgcct atgcaatcta caattatacg aattacaccg gcgatacgga
    56461 atatttgaaa aatgaaggac tggaagtttt agcagaaatc gcacgcttct gggcagaccg
    56521 tgtccatttt tcaaaacgag caaataaata catgatccac ggtgtgaccg gcccaaacga
    56581 atatgagaac aatatcaata acaattggta tacaaatacg atcgcagctt gggtcttgcg
    56641 ttacacgcga gaaaattatt taacttatca aaaagaagct gctatcacta tcagcgaaga
    56701 agaactagcc aaatggaaag atatcgcaga gaacatgtac ttcccagttg acgaagaatt
    56761 aggaatcttt gtccagcatg acacattctt agataaagat ctcatgcctg tatccgatat
    56821 tcccacaagc gatcttcctc tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg
    56881 cttcatcaag caggcagatg tactacaggg gatttatttc ttcaatgata cttttacaaa
    56941 agaagaaaag caaaaaaact ttgatttcta tgaaccaatg actgtccatg agtcttccct
    57001 ttcgccaagt atccattcga ttttagcagc agaattagga atggaaagca aagctgtaga
    57061 aatgtaccag cgaacagctc gcttggattt ggataattac aataacgaca cagaagacgg
    57121 cttgcacatc acttccatga ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat
    57181 gaaaactgcc catgatcagt tgagattcgc accatttctg ccaaaaactt ggaaagctta
    57241 ttctttccat atcaattatc gcggacgttt attgttcatc tcagtcacag atgaacaagt
    57301 tactttcgag ttattagaag gttctcctct agaaatttac gtctatgaac agtcttattc
    57361 tttagcggat cgtctagaaa taacgctaag aaaggaggaa gcaaatgttt aaaggggtat
    57421 tgtttgattt agacggtgtc attaccgata ctgctgaatt ccattatcgt gcttggcgaa
    57481 agcttggtca ggaaatcggc attacgatcg accacacatt caacgaacag ctaaaaggcg
    57541 tcagccgaga agactcactc tctttgcttt tagcttatgg cggaaaagaa cactcttttt
    57601 caaaggaaga atttgcagaa ttagcgaaaa gaaaaaacga ttattattta gagatgatcc
    57661 agacgatcga accaaaagat gtcttccccg gggtcgtgcc tttactggat tctttaaaag
    57721 aagaaaaaat caagattgct cttgcttctg caagtaaaaa cgggccattc ctacttgaaa
    57781 aaatgggtct tacaccttac tttgatgcta ttgccgaccc ggcagaagca acaaacggaa
    57841 aacctgcgcc agatatcttc ctccttgcag caaaagctgt cggactaaaa gcagaagaat
    57901 gtatcgggat cgaagacgcg caagccggta tccaagccat cctctcaagt ggcgctcaac
    57961 caattggcgt gggacgtgct gaagaccttg gagaggagat cccgcttgtc cctgatacga
    58021 cttttcttac gatcgattac ttgaaagaaa agtggcatga ccatggataa tccagagatc
    58081 tccgtcgtac cagttccaga gactgaaata aagaaaatca cattgaagaa cgactactta
    58141 gaagtcgttc ttctcaatta cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa
    58201 ggaaaatcag aaaatgtttt attgtcttac gatcgtttcg aagatgttct aacagacaaa
    58261 agcttttttg gagcaactgt cggacctgta gccggaagga tccgggatgc ttcttggggt
    58321 gagcatagat tggagaaaaa ctgcggatca caccatatcc acggtggcac gaaaggctgg
    58381 tctttccaat tttgggaagt agaagttttc aaaactccat catcaatagg tgtggtcttt
    58441 tacttaaaag atgaattttc tacctatcca ggaccaatca ctgctacgat cacttaccgt
    58501 ttaacaaaaa acgaattaga gatgacaacc tctgctagta gccttgttga aaccatctgc
    58561 aatccgacca accatgccta cttcaacctt tcaggaaatg gaacaagaga catctatgaa
    58621 catcagttaa cggtcttttt agatggtgtg cttgaattag atcaagaaaa actgcctact
    58681 ggaaattgga caaaaaaaga aaaactgcca atcgacttta gaaaatcccc aacactgcaa
    58741 gagatccttg catgttatcc agatggacta gatgatgtat ttcttttgca tcatcctagg
    58801 ctttccaaaa cttccttaca attattcgag aagcattctg gtcgccaaat gactatagca
    58861 acttctaata aaagtatggt attattttct acgactggat tcgaagctga tttctctgtc
    58921 aatggaaaac agatgcattc aaactacgga ttagcaatcg aaccgcaaga aatccctgat
    58981 atcgttcatt tccccaaatt tggatcgatc aacctccatc ctggtcaaga acggatcagt
    59041 cagacgattt atcggttctc tgcacaataa caagagaaag gtgtttttat catggctatt
    59101 accgtaaaag atgtggcgaa aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc
    59161 aacgatcatc ctagtatttc cgagacaaca aagaagaaag tccgaaaaat tatggatgaa
    59221 ctaggttatg ttcctaacat gactgcccgc aatctaggaa aacgcatttc cagtgccatc
    59281 ggcgtaatct taccccctct tgattcaaaa gagcggatcg gtaacccatt ttatcttgag
    59341 atcatggaag caatcaacga agaagcgcgg aattatgata tgactacagc aatcgctact
    59401 gctaaaaatt ttgaattgct gttagagaac gtccagcgta tgcatttaca aaaacaagta
    59461 gatggtttta ttctagtgta ttcagatagt caagatactg tcattgatta tttataccaa
    59521 aaccacatcc ctttcacttt gatcggacag ccttatcatc atgagaacga aatcgtttat
    59581 gtagataatg ataaccagct tctaggtaaa caagcaacag aatttttgat ccagaatgga
    59641 cataagaata tcctgtttgt gacaaacacg actcatgaaa gtttgtattt cgaacgatat
    59701 ttcggttatc aaaaagcaat gatgatggct ggtcttcctg cgttatccag tgtagtacta
    59761 gagcatccgg aggattatct gtcctttgag aaaacgttga aagaaaacga agccacagca
    59821 ttggtggtca ttgacgatat cttcgccctt cgcacgatgc agttggcgca aatgtatggc
    59881 tatgtcattc cagagacact ctccgtcatt agcttcaaca actcgatctt ctcaacatta
    59941 acacaccctt accttacaag tatcgatatc gatatctccg aattaggacg tgtcgctatg
    60001 caaaagctgc gcgaattgat ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct
    60061 catcgcttga tcaaaagaga gacagtcgtt cctttagaaa acccatacta aatagctgac
    60121 tcactaaata cgctgaccat ttatcctatg gtcagctttt tatgtaaaag aagatactac
    60181 ttctaacaca ttgtttatct gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt
    60241 ctcacgctct aaacacgtat aaacggcgga agcagaagca actccttcgg aaataagctg
    60301 aaattcacaa aaatttaaaa agcaattttc ttgagtttct tcttatttct tggagttaaa
    60361 cgcttctgtc ccagcctctt ttttccttcc ttcaattttt tacacgtcga cgattcaata
    60421 aaaaaccgcc taatattgta aagacaggtg caagaatcga gaaaaaggcg aatggcaagt
    60481 aatgcaacgc gctaactttc atcgttccca taatgaatgt gccgctgact ccccacggta
    60541 cgagagaatt gacggtcgca cctgaatccg ctagtgttcg tgtcaaatag gtttttgata
    60601 agccaacgtc atcaaaagaa cgcttgaatg tttctcctgg aagaatgatg gacagatact
    60661 gttctccaac gatcagattg attccgatac agctcaaggc agtaaacccg actaaacgag
    60721 aaggtcggtc catcttttct tttaattctt gaacgatggt atcaacgatc agatatttga
    60781 tcagtaaccc acctaatgcc aatgcaagta aaatcaaact agcagaacct aacatactca
    60841 tgatcccgcc tcgagaaagc agttcatcta gctttggatc aggtgtattt gcgacataac
    60901 cattcatcaa gatgtctgct gcttttccga ccgtcaaact tttctcatgg ataaaagaaa
    60961 gaacgagagc agctgtcgat cccgctagta atgtagggat agccggaatt tttttgattg
    61021 cccccccaaa taaaataact agtggaatca atgtccaagg agagatccaa aagctcgctt
    61081 tcatgttttg gaccatttca ttgatactag ctagatcggc tgaatgactg ttatgtccta
    61141 atatcgtata aaaaatcaac gaaaggacga atgctgggaa atccgtataa cgcataccag
    61201 cgatatgttc gaatagatta actcctccaa tacccgctgc caagttggtc gtatctgata
    61261 aaggcgagca gttattccct aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat
    61321 cattgaatcc aagtaaatga ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg
    61381 taaatgaact gccgacagtc actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa
    61441 agaaacgcac agaaacaagt ttaaatccat aaaccataat cgtagggatg gttccagata
    61501 aaatccatga agcaactaag acaccaatca aaagaaaaat caggattgga atgatccctg
    61561 gagtgattcc tttgatgatc ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc
    61621 cataaaacaa caaacacatg aaagctgcaa ggatcgggat atgtggcgag agctggaatc
    61681 cgatgatcag tactcctagg agtgccaaca acacgatcaa tacagcgata ctttctttta
    61741 aagaaatatt tttattcatc gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa
    61801 aaactattca cttggcagga cctcctacta aatcagcgac aaaacgtcgg ctgaaagact
    61861 tattttttta tttttcttct aaactttttt ctacctcaat cccccaagaa tctttcttga
    61921 agtgattgat gatcaatgga ataacgaaca tgaccactgt gatagctgct actaataatg
    61981 catcactcaa tgtcgctgta cctaatgtag caaatacgac aacgatgata gagaagatca
    62041 gcatgcagct gactacccaa gctaatccgt ttccgttttt accaattcgg taaggacgtt
    62101 cgaattctgg atgtttctta cgcattttaa gcaatgaaac agcaatcaaa atgtacacga
    62161 tacaataaat aacagttgta gcatttgtaa gtgtcaagaa tacagcactt acgtcatcca
    62221 ttacaccata aagcaaagca aataatgaaa taacgattga ttgtgttaga acgacatttc
    62281 tagatacgcc gtatttgttt tctttatgga atccgaattt tggtggaagg aatccttcac
    62341 gagctacttg gatgattgtt tttgatggtc cagtaaccca agctgataat tgtaaaagca
    62401 caccaataaa taccatgaag ctgaagatat ttccgatcac ttctggtaag cctaggattt
    62461 gacagtcaat caagataggc tgcgtgatat ttgctaattc catttttcca tctggtacga
    62521 catttgctac aagcatcgca ttgatgacat tcaataaaac taatccgatc aaagcgataa
    62581 atacgccttt tgtataattt tttgttgcgt ctttcaaacg aggcatgtaa acagaactca
    62641 tttcgatccc aacaaaaata aatgtgatac cagctaagta tttcaatgaa tcaatatgtt
    62701 ccaagtcagg gaaagctttt gaccatgaaa agtcacctag atagccgttt gagactagtc
    62761 cgactttgaa ggctgccaaa acacctaaaa cgaacatgat gactactggg atatataccc
    62821 ctagccatac accgatgttc ccgccgactt ttgccatatc gaatttcaag ttcaagatcg
    62881 tgatgatcca gtagataact aaaatacaac ctaaaacgaa ccagtgatta ttgccaagtt
    62941 ctacattacc aaatgtgttt cctaacaatg gtcctaaagt agaagcgacc attaccatac
    63001 ctgggaacat ttgcacccat aatagccatg caacgacaaa tccccatttg ctgcccagtg
    63061 ctgtttttac ccataactga ggaccgcctt cttgaggaag catcgtggaa agctcaccgg
    63121 aaaccattga tatcggaccg gcaaaaaaca aaactgcaaa aattgaataa aaaatcaatg
    63181 tccaaccgac tgcggctaac gtaggaatac tccgaaccgt tgcacacagt gccattgtca
    63241 gaccgataaa ggtcccaaga cccattttac ttacttcaga tttactcacc gttgacactc
    63301 tccttgattt tttggataaa ataacggtgt ctttgaagta gtttcagcaa taaacgaaac
    63361 aattcagcga atatttgagg ggattctttg agtttattgc ttgaagggtt tcttcaaaga
    63421 caacctcaaa acaaagggat cgaagagctt aggtctcccc ttgctcttca gatccaaaca
    63481 tgcgtcagaa acagttagta ttcaagctgt tttctaacat gattaccttg cttattttgc
    63541 ttcgcttgcc aataattggt ctgcgtagat tttttccaat ttttcttgaa gagctgcttt
    63601 gattttttca gcatattctt caaagtgttc ttctttgttc atgtatggtg tcattacgct
    63661 ggcacgcaat acagttactt ttccggcacg gttccattct tcatctgaga atcctagttg
    63721 gttcacaaat tgtaatgggc tgttgccata gtctgggata gcaaagtcag tatgtgaagt
    63781 taagaactca ttaccgtaaa tgcttccttt cacgtatgaa gaataatcat atacgtcatg
    63841 gttcaattta ttcattgcaa ctaagtcatc gttgcctttt tctttgaata catagtctac
    63901 catattgaaa tctggataag ttaatggatg aacttcgatt tctttgtctc ctactttaaa
    63961 tgacaagtca ttcaagaagt tgtagaaacg atgtgcacct tcgattgatg cacccattaa
    64021 tttaccatat cctgtgacat tcaaaggtaa gacatggtgt gctgcccaaa cgctggctgc
    64081 tgttgcccct gcttttgaac cttccaagat ataagcccca agtaatgctg ggatatctgc
    64141 gcctttttca aatacatatg tcgcaaagta agagataacg tctctcatgc ggatatcttt
    64201 gatgacgata ccaccggcag aatatggaac atagcccatt ttatgtgggt caatcgttac
    64261 agattctgct tcttcgattg ctttatatgc actgtgtact tcttctaaga tataatcttt
    64321 gttttctgta aagacattgt atttgtaatg aacatctttt aaatcttcaa atggaatgaa
    64381 gttgttgtct tcgtctaaga aaatcgcacg gccgtatccg ccataagcag catctacgtg
    64441 taagtagaag taaataccgt ctttttctaa tacgcgacgc aatgcaacga ttttatcgat
    64501 accgtcgatt gctccttctt ctgttgatcc tacaacaccg actacaccta ggattggtgt
    64561 tttttcagct gctagaccac gaacgatttt ttcaagttcg ttgatgtcca tgcgatagtt
    64621 gtggtcaact ggcactggaa tcacttggtc taaaccgata ccgatgatgt cggcagcttt
    64681 tagccatgaa tagtgtttag tttgtggaac caaccatttt cctaattttt ctaaatgttt
    64741 tccgctacgt gctgagtgag ctttgatttc gtcgattttt tctgggacgc tgtctaacag
    64801 attcatgatt tcttctgttg acaagttcat taattcccaa tcgctttttc cagcaactag
    64861 ttcaggtgtc acttctttca ttgcaagcgg caatgatttg atgttgcggg cataccaaag
    64921 tccttctaag tttgctaaag aaccatcagc aacgatgtgg ccccagccat ctttatagct
    64981 cattaatttt gcaaattcca ttcctacttc ttcttccatt tggcttgttg caggagaaga
    65041 ttcataggca acgttattac cattccatag catagcaaaa ttgtacgcta gtaaagatgg
    65101 catcaatgtt tcagagttca tatgacccca gtaacgtccg gcattatgcc aaggaacgga
    65161 atgggtacgc atccgcgctg aaatttctga tagaacatcc tttgttttat tcacagtgtg
    65221 ttcaaaacta gcactgcttt tttcttctgg cgtaataata ggcatgtctt gaggcatgta
    65281 gttttgacgc cagcctaaat gttcatccac caattcattc aacaaagctt tatagatttg
    65341 gccgttctca gctttgtccc caatgaatag ggcatttagg tttagatctt tcgacaatga
    65401 ttcactcata gtgaaaccct cctaaaaaaa tatttaaatt ttgtcgagcc ctacattaca
    65461 cctgttcttc tattctgtaa actaagtttt ggcctgattt ttaatcatat tttaatatgt
    65521 atcaattatc cgaattgttc acacttttga aataattaaa aaaacgaggg aatttacaaa
    65581 tgtaaactcc ctcgcaaaga ttgtattaac gacaaccagc gttctatttt aggatgggtt
    65641 tcaaaattgg aatgttgtaa tagtatttcc ctttttcaag gtaaaaactt actaaattct
    65701 aatcaattac tttggccaaa aagtaatttt tcttaccttt tcgaataacc acaaacttac
    65761 catcgaattc atcggatgga tttatgacaa aatttaaatc ggtaacgcgg tcaccgttaa
    65821 tacttatagc tccgtttgaa acatcttcac gtgcttgacg tttagatggt tcaatttttg
    65881 tggaaacaag cagttccacg atattttcag gtgtgcttga aatttcaacg ttcggcattt
    65941 taccgaatcc ttgcgcgatt tcttccgcat ttagttcttt aatatttcca gagaacaatg
    66001 cttgcgttat tttttgcgct tctttcaagt cttcttcact atgcacaaat cttgtaactt
    66061 cttctgccaa acggcgttgt gcttcacgtt tctcaggttc tgtctctact tttttcgcta
    66121 agtcttcgat ttcttcttga gacaagaaag taaagaattt caagtatttc actacatcgc
    66181 gatcatcttg attcaaccag aattggtaga actcaaatgg tgaagttttc ttcggatcaa
    66241 gccaaacggc accgcctgca gtttttccga atttcgttcc gtcagccttc agcatcaatg
    66301 ggatcgttaa accgaacgct tctgcttctg gtccttctaa ttttcggatt aggtctaatc
    66361 cagctgtgat atttccccat tggtctgctc caccgatttg caattgcaca tcgtatgttt
    66421 tatgcagatg caaaaagtcg atagattgca ggatttggta agtaaactct gtaaatgaga
    66481 ttcctacttc caaacggctg gcaacgatat cttttgctaa catcgtgttg atgttgaagt
    66541 ttttaccgta gtctctcaaa aattcaagta aagagatttt tgacaaccag tcgtagttgt
    66601 tcacaaatgt gatatttgca tctttaccaa acaattttct catttgattg gaaagtgcgt
    66661 ccacgttatg ttgcacttgt tccatcgttt gcaacacacg ttctgttttg cggccgcttg
    66721 ggtcgccgat cgttcctgtt ccgccgccaa tcagaatata tggatgatgg cctgccaatt
    66781 ggaatctttt catcatcata aatggaatca aatgtccgat atgcatcgaa tcaccagttg
    66841 gatcaacgcc gcaataaagt gaaatgctct tttcttcaac gagctcgcga agtctttctt
    66901 cgtttgtttg ctgattaatg gcgtcgcgcc aagttaattc atcaataata ttcattttac
    66961 tgacctccgt gtagtttttt tatgaaagca ggaacctgct agcatgtgta attaattatt
    67021 tcttcaggat aggataggaa ataaaaaaag tcccttggca tataagccaa gggacgaaat
    67081 ctcgcggtac cacccaattt gtatgtctag atatccaaca tacctctcga acatttaacg
    67141 ctgtcgcgtg gcaatcactg ccaaagactc ctgaaatgta attcgcttta aagcatgtac
    67201 tggtttgcag cgaccaccag ctttctgaaa cattggcttt aacaactact tcgtttcgat
    67261 cttcgtcttg ttattattaa tatgtgtcta tcatagagcg caactttttc tttgtcaaca
    67321 gaaaaaggat atttttttta aattcctcat tttttctttt ttgaatccgc tctaattccg
    67381 aacattttat tttcaattat tgttaaaaga aataactttt tctgtatttt ttctgcaatt
    67441 tatcgagaag tcactttact ttgcctctta ttctttcttc gttacaataa ggaatgggac
    67501 aagtaaaaaa atgaaaaagc aagggacgag gtatatggaa aaaaagcaaa aaattatttt
    67561 aggagccact tctgctttaa ctatcgttat tttaattcta agtttgtttt ttgtcatccg
    67621 atcatctaca aaaaaagaac caatcattcc tgaaaaaaaa ataacagaaa aagcaaaaga
    67681 accgacagat gataagcaac aactagttac tcaagcaaaa aatctggctc aaagctacga
    67741 ttatgaccaa gcgattgcgt tattggaaaa agatagtaag aacggatcta ctgaaacaca
    67801 acagcttctt caaacactaa aaaaggagaa agaacaactg atcgactggt ctgatcctac
    67861 tcagatctct catgtctttt tccacagctt gatcgtggac ccgcaaaaag ccttccactc
    67921 tcaacaggca caaggataca aagattatat ggtgactgtt gaagaattca accgttcgat
    67981 cgaacaactt tatcaaaatg attttgtttt agttaatttg aatgatttga tccaaaaaga
    68041 cgaacaagga aatctttcct ttaacgggct tgcgctacct gaaggaaaaa aaccattggt
    68101 tctttcacaa gatgatgtca gctattatga atatatggat aattccggtt ttcctagccg
    68161 attggtcgta tcaaataaca atgaaatcaa aaatgtctat aaagagaaag ataaagaaga
    68221 aatcggtgac tttgacatgg ttcctctgat cgatgccttt gtcaagaaac atcctgattt
    68281 ttcttatcga ggcgcaaaag gtaccttggc cctgacaggt tacaacggag ttttaggtta
    68341 tcgaacatca aaatctcaat atggagacaa tgaaaaaaca aaaaaagaga ttaaagaagc
    68401 taaaaaagtg gcggaacagt tgaagaaaga cggctggagc tttgcttccc atacatgggg
    68461 tcacttgaat atgactcaag cttcactaga agatgtaaaa aaagacaatg aattgtggca
    68521 gcaagaagtt gcacctatcc taggaaaaac aaatattctg atttatccat ttggtgcaga
    68581 catcagcgac tggcagcctt attcagcaga aaatcaaaaa tttgcttact tgaagagtca
    68641 aggtttcgat attttctgta atgtcgatgc ttctacacca gcttggggac aattaggtac
    68701 ggatttctat cgaaatgctc ggatcaatat cgatggcatt cgttttcaat ctgatttaga
    68761 cggaaaaaat cctattcttg atccattcat caatgtccat gatgtctatg acaaagaagc
    68821 acgcgaatct gcttgatttt acgtcattta tgaatcgagt agaggggaaa gccaactgac
    68881 cttccgcccc tctcacacca ccgtacgtac ggacccgtat acggcggttc aataagttga
    68941 gtgtacgaac tggtacttca aggagatgtc ctcatatcct tggcgggcca gttctttgtc
    69001 tgttaatgat cgatgaagaa tatgactttt ggagactgac caaatacctt ttcttgtatt
    69061 tgaccattca tacgctttcg ctttatcgat ccctaaacga atcagatttt ttcttttggt
    69121 tcttggattc ttccattgtt tccaaatata ttgtctgatt cttcgcttca accaaccgtt
    69181 taactccctc atgaattctt tcatctcacc aattccatag tagttgatcc aaccagtcat
    69241 tagttgcttg atttctttta aaataagttg gatacttctt ccacggtttc ttttagtgag
    69301 ttgtttgagc ttgcttttca cacgctgttt tgcttttgcg tgagggcgaa ttttaatacc
    69361 tttctttgtt ggcagaatgc agaatccaag aaactttcgt ttcaatggac ttcctaccgc
    69421 actcttttct cgatttaccg tcaactttag ttgttcttcg agaaattgaa tggatttctt
    69481 catcacacgc tgtcctgctc gtttgcttct aacatagata ttgcagtcat cggcgtatcg
    69541 aacaaattta tgtcctagat tttctagcac ttgatcaaat tcgttcaggt agatgttact
    69601 taacagggga gagagattgc ctccttgggg cgttccttct tcagttgttg tgatgagtcc
    69661 attttccatt acaccactca ttaagtattg acggatcagt tttaggactt gtttgtcctt
    69721 tactttccgc tcaatgaaat acatcagctt atcgtgattt acggtgtcaa agtaggcttt
    69781 catatcaatg tccaccacat atcggtaacc ttgttcataa taagattttg ctttcttcac
    69841 tgcgtcatgt gcgctgcgat ttgggcgaaa cccataacta ttatctgaga attctggttc
    69901 aaatagcggt tgtaacactt gattgattgc ctgttgaagc atacgatcaa taaccgtggg
    69961 aattcctagt tttcttacac cgccgtttgg tttcggtatt tctactcgca aaacaggctg
    70021 aggtttatag ttcccttggc gtaaattgga tagtaattcc tcgcggtgct ctttcaagta
    70081 aggaagcagt tgatcgacgg tcatcccatc tgcaccagcc gcccctttat ttctgactac
    70141 ttgaagatac gcttgattga gattattccg atctagaatt ctcccaaaca gattttctac
    70201 accatcttgt ttcctctgtt caccgcaaga agtgctgcgc acttcatcat acccttcgtg
    70261 ttccacacta gctctttgat gatagccaag ttttcttgtt ttctgcggtc gtcgcatctt
    70321 ctcacacctc ctaaattatt caagattctt attgttcagt ccttcgtaac ggtcagtgtc
    70381 gttactacta tgacctcggc tgacttctca tgattcacca tcatgtcgcc atgatcgttg
    70441 tgctggtttg ttttcattca acgcccacaa caccctcatg agatctcccc cggtaagagt
    70501 gaaaactttc ctcccatgta actgctagat ttactgtata ggtttcgggc agtattggac
    70561 tttgtcttgt gatgaagact tatccgacct aattcagcct tgtatctagt ttctgttcgt
    70621 cagttcagga gtttgcgtcc gacttccttc agccacaacc tcacgattgc cgccttgtct
    70681 ttcgctaaca gttcctactg ccaagcctgt agtggacttt caccaccaag ttttcaccca
    70741 tgcagggcgc acaccaaaaa agccgaactg acattttatc gtcagtttcg gcttttttat
    70801 gctttatttc ttcttcggtg cgattccctt gatcaggact tctgtccatt ctgctgtata
    70861 tggaagcagt tttgcgtata tctccggctt agaaattcct tctaccattg cttgaaaata
    70921 gaggatcaag aactcatccg aatatttcaa atccactttt ccttctttcc gccctcgatg
    70981 gaaaagatca agcatgatac tgaaactttc ttgcgtatac gcatccatcg cgtcagaaaa
    71041 ttcttttttt tctttttttg tatagtatgt catcaaatcg atataaaatt gtttgttcac
    71101 tcgattgaga taacggattt tgttttcact catggcaatc agtgtttctt caaatggaag
    71161 attttccgca atcaacgctt tcgcttcttg ccctaaatca ttcataaatg caaaaaatac
    71221 ttcataaatc agcccttcct tgctgccaaa atatttaaaa atcgtcgctt tacttacctg
    71281 agcgttctca gctaccttat ccattgtcac tccagcggtt cctatgtctg tgttcatcaa
    71341 gtcaaaagct gcttgtaaaa tctctttctt tttctcttgt gctctctttt caaaaccgtt
    71401 catttgtaga gcctcccgta attttaaact tttataatat atatagttca aatttattct
    71461 gaggtttatt ataactatct attgagaaag tatcaagcta gcaattcctt gtttatgaat
    71521 tcattgacag aaatttataa aaagaatatt atgtactaaa ataataaaaa aagttttata
    71581 aaaaggtata aaaccttttt attaaactat ttaatcttgt ttagtatata tttttagaaa
    71641 aaggagtggg gtttttatgg cagaaatcgt caaagtcaca catttgaaaa agagctttgg
    71701 tcgtttcgaa gctttaaaag atgtctcttt caccgtacat tcaggtgaag tcgttggatt
    71761 tatcggacca aatggttctg gaaaatccac gacgattcgg acacttttag ggattataaa
    71821 aaaagacggc ggaacggcaa cgatttttgg gcaagatgta tgggaagata gtttatctat
    71881 ccatgaacgt ctttcttatg ttccaggcga tatctctttg tggggaaatt tgactggagg
    71941 agcgatcatc gatctcttca tgaagcttca tggaaaaggg agtgctcaaa aaaaagatga
    72001 actgattcaa cgtttctctc ttgatcctaa gaaaaaagca aaaaactatt caaaaggaaa
    72061 tcgccaaaaa gtgggcttga ttgctgcttt ggctgttgat tcggatctct atttatttga
    72121 tgagcctact tctggtttag atcctttgat ggaaacgatt ttccaagaag aagttgaaaa
    72181 attgaaagaa cagggcaaat ccatcctact ttcttctcat attctcagtg aagtcgagcg
    72241 actagcggat aaagtcgtga tcatacgcga aggaacactc gatgaagaag gaacactcga
    72301 tgaattgcgt catttgactc gttcgaccat cactatagaa acagaagcag agaatagtca
    72361 cttagcacaa ttgaacggtg tccatgactt tgtgcaaaaa ggtcacctcg ctcaatttgc
    72421 tgctgataac gaagctttcg atgtgatttt agctgaagcg tccaaattag gtgtgaaaaa
    72481 atttgaatct attccaccga ctcttgagga tttattcatg agacattatg aaggatgagg
    72541 aggaagcgaa atgactgata aattttcacg ctggtccacc cttttttctc aaaaccttag
    72601 ccgtgactgg aaaaagatcc tgatatggct agtcggtgtc actgcttttt ctggtggttt
    72661 tgtccctgcg tttgaagaat tgacttccgg acaaggatta gctggattat acgaaacctt
    72721 gcaaaacccg gcaatgattg caatggtcgg accaactcca gcttcgaatg ctgctgagta
    72781 tactttagga gcgatgtatg caaatgaaat gttgcttttt tgcagtttgt ttgctgctgt
    72841 gatctctgtt ttacacgtgg tgagccaaac gagaaaaaaa gaagaacttg gtctgattga
    72901 acttattcgt tcctttcgag tcggacgtca agcaaactct ttagctgtca tcgctgaaag
    72961 tattctgatc aatttgcttt tagcaatatc gatcagttgt acgatgattt ttttccaagc
    73021 ggattcgatc actgcagaag gtgcttggct ttttgggcta tctgtaggta gtatggggat
    73081 tttggggagt gccgttgcac tgtttgctgc acaactgatg ccaagttcag ccagttcgaa
    73141 tggtctgtct ttagcgtttt taggtggact ttaccttcta cgagcaataa cagatgtagc
    73201 tgattcctcc ctttcgatgt ggaaaccttt aggctggtct tatttgacct atccatttac
    73261 aactaacaac tgaattcctt tgctttattt gtttttgttc agtctaatct tgataatcag
    73321 ttcatttgtt ttagaggaac atcgagattt gaacaccggc tatatccccg aacgtgaagg
    73381 aaaaccgtat gctaaacgtt ctcttttatc cttgcgtggt ttattattct atctaaataa
    73441 agggatgaca atcagttggc ttatcacttt tggtttgatg ggggcggcat acggctctat
    73501 ctacgggaat atgcaacagt ttttagaaag taacgactta atgaaacaga tgtttatcca
    73561 atctggcgtc tcaattgaaa aaaactttac aagcacgatc atggtcgtct tgatttgtct
    73621 agctgctatt ttaccaattg ctagcatcaa tcgtttattt acagaagaaa cccacggacg
    73681 tatgaatcag ctgttcgcaa caaaaatttc taggaaccaa ctttattgga ccaatatgtt
    73741 actagctatt ctatttgcta tattggcaat cctgattagt tctggtgcac tagggctttc
    73801 tgctttggct tcgatgaagg atgcgaaaga catggatcta ttgatgtttc ttaaagccgg
    73861 gttcaatttc ctgcctgccg ttttagtgtt tatcggaatt agtggctttc tattcggatg
    73921 gcttcctcga atcggtaaac ttgcttatat ctacttagct tactcctttg cactcaatta
    73981 ctttggcgga attttggatt taccagaatg gttttccaaa acagctgtcc ctagctgggt
    74041 tccacggatg ccaaacgatt cttttgatct ttccgtgttc cttacgttat catgtatcag
    74101 ccttgcactg ttcgtccttg gttctattgg ttataggcga agagacttac tagaaggata
    74161 ataaaaaagc atgtacaatt tcatgctagc ctccaaatct gggtcaaaaa tccaacattt
    74221 ggaggtcatt tttttattta caagtcttct aactgctctt tatcaatgat ccagacttct
    74281 cgattctttc tttttacata accattctcc tctaataatt taaattttct agataaagtt
    74341 tcgggggtcg taccgataaa tgcagcaagt tctttcattt tcattggcag gaaaaagtga
    74401 tccttccctg tcgctttagc taagtcaagc agatagacag cgactcgttc ttctactttt
    74461 tccatcatca aaaaagttgt ctgctgctgt acttttgtta atttctcaac gttgattgct
    74521 aaaagtttca gactaagggc gggataatgc agtaagagtt ccgagaaatc tttttgtctc
    74581 aatatgcaaa tttccgtttt ttctagtgct tcgccaaata aagactcatt tggaacgcct
    74641 agcaactggc tctctccctc atatccgccc ggctctatta ctcgtaaaag ctgttccttt
    74701 ccgctagcag ataattgata cactttcata ctgccggagg ccacgatcac taattgaggt
    74761 tctccattcg gcgcaagaat ctgttctcct ttttccacat agtgatgttt gacaagtgcg
    74821 tgtacttttt cttgatcttg ctgtactaag tgattgaaca aaggaacaaa tgaaacacat
    74881 aaatgatcat gactcataat ctctcctcct tctaaacagc tatttctaat aaagaaacat
    74941 aacttccctt attccaactg ataattttct tttatcatag cataaacgtc aagcgaacca
    75001 gataccagca aatagagtgg atttcttttt gtcccttgct ttattttcct tcttttgaga
    75061 ccttaagtaa ttgattccta tcaatttttt aaatatttac tttttgaata atcaaaaaca
    75121 aaccttcact cgcaagccga gagtaaggtt tgtttttcgc attgcttatt tcgtgttgtc
    75181 tattgaaatt tttccgcacc tagcttttga tatagtggtg ctagttcttg taaggtttca
    75241 gcggttattt gctcaacgtg ttcgatttct tgcggataga agaatagttt acccatttga
    75301 aaggttcggt ctctcatcgc ttttttcaac tcttcttgtt tctttgtatc ggacaaatag
    75361 tcagacaaag ctaagtggtc gtcaagttca tgttctggtt gcggccatat gtaataatcc
    75421 ttgatatcga ctgttttgct ccactctttc agtatcgcaa gcaactggtt atacgctgca
    75481 atgctaccat atcgttcttc gcttttataa tgttggtaca tcaaatagat ttggtattgt
    75541 ttttgattca acaaaactgc tagacaggca ttctcgtctt gacgagattc gcttcggtac
    75601 gcgctccaaa aatgactgcg taaattccag ccattcgtcc agctttcgat tttaggtttg
    75661 ctcatgccgt acatatcagg caaaagtgca gctgtctgga gctgaatctc tttccatttt
    75721 tgccagtgtt ctttatattc tttcttgatt tgttcaatct tatctgttgg atggctctct
    75781 ttgagctgtt tgaatgcgta gattggtcga tcaaacaact gataatctga ttttccaagt
    75841 tccattgttt cgcctctttc cgttatcttt tgtattctac ctgaattttg cgatatggac
    75901 aaatagaggt tgtgaaaaag ctgttctgca tcaagtcata agaacagcca aacaaaaaac
    75961 tgcgatgagc atcaccgcag ttttattgaa cttaaggttg attgatcaca tctaaaagcg
    76021 ttgaaaataa aatggcacta gaagcagctc ctgggtcgat atgtccgatt gcacgctctc
    76081 ctagatagga agctcgtcct tttttagcta ccaagacttt ggtgtgttct tttgcctggt
    76141 caatgatttc ttttgttaaa ttccctgcct tcaatgcctc tgtgactggc agccaaacat
    76201 ctaacatcgt tttatcgtca gcttctgcct ttcctcggct ttgaataccc gctgttccct
    76261 gctcgatcac ttcgcctaat ttttcttgag aatcaataga atcgacctct tttgtcgctt
    76321 tcatcatgtt catgaatgca gatccgtata acggaccaga agccccaccg actttcgata
    76381 tcatcgccat agagaatact ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa
    76441 aagcttcttc ataagctgaa acccctcttc ccatgttatt tccatggtct ccatcgccaa
    76501 tcggggtatc tagatcactg agatattgtt tgttctgatt gattgcctct gcaaattgcg
    76561 tgagccattc ttggatttct tgaacggtta gtttcatatt atcacccttt tatcttgaga
    76621 ttttttacca tgaaatcgtc tgtacattgc tttctaaaga atctttccat tgtgtctccg
    76681 tcaagtcgat cagtgttaga gaaagacctt gcatatctag tgaggtcata aaattaccga
    76741 cttttcggaa aactacattg acgcctcttt cttccaacag agctaaaaca tcattcatga
    76801 acacgaactg ttccatcaaa ggcgtcccgc ccattccgtt gactagtaca cctgcttctt
    76861 ttggtaaatt cgagtaatca tctaaaatct tagtaacaag ttcctttgcc agtactttag
    76921 aaggctgcat cttttctcga cgatagcctg gctcaccgtg gataccaaca ccatactcga
    76981 tctcatcttc tcctagttcg aatcctggtt tccctacttc tggtacagtt gctgctcgaa
    77041 gcgctagacc gatcgtctta gttgcctgta cgaccttttc tcccagttct ttcaactcag
    77101 ctagggaagc accgtttctt gctgcatcgc ccacgatttt gtgtacaagg acagtacctg
    77161 caactccgcg ttttccagca gtatacgtac tattttcaac ggcgatatca tcatctacga
    77221 tcacactttc tacttcaata tcgtccatag cagctaattc ctttgccata tcgaagttca
    77281 atgcatcccc tgtataattc ttaacgatca gcaaaactcc ttttccacta tcggcttcct
    77341 taatagccgt ctgaatctga tctggagttg gagaagtaaa cacatcacca agcaccgctg
    77401 cacttaacat cccttcccct acaaacccgg catgggatgg ttcatgtccg cttcctccac
    77461 cagaaaccaa accaacttgt tccgctttct gattcttggc tacaacacgg gaagattcta
    77521 cacgatgaac aagttctgga tagcttttta ctaatccttc cagcatttct tcgacaatat
    77581 tgcttggatc attcatgatt tttttcaatt tattcccatc ctttctttcc ggcattttgt
    77641 gcttctgtta agatttgctg agtatcatct gttataccgg cagtgatggc tgctgcaaaa
    77701 gctccttcca ctaaaggcgc atccaccaga tgataacgtt gctgctgctc ttcttccaac
    77761 atgtcttttg ccagttccga gttcaatacc gcgctaccga tatccgcaaa aacaaaataa
    77821 ctagctgcat cgcttgattc attgactgct tctacgattt tcatcgggtc tgatccgatc
    77881 tcgccattta ttccgccgag agaaaaaatt tgcacctctt cggatttttg catctgctca
    77941 atcatttctt taataccatc tgttactttt tgactatgag aaataagcaa aatacttttt
    78001 ttcacaagaa tcttcctttc ttttacatag ttgctaacga ttggtttcgc tttcattaat
    78061 gaacaattca agtatataat aaatttctaa ttttgggaaa atatgtgtag tgcttcttga
    78121 agtttgttat actttaatta aaagagagga gtgatcgaat gattacaaca acaacgaaca
    78181 gtgtagaggg aaaagccgta gtagcttata aagggatcgt ttttggtgag gtcattactg
    78241 ggatccatgc atttaaagac ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa
    78301 aaagctatga aaacgaacta atgggtgcaa gagaagaagc acttgccgaa atggccgaac
    78361 gtgcaaaagc attaggagct gatgcgatca ttggcgtaaa aatggactat gaagtattag
    78421 gcagcgacaa tggcatgctg atggtcacat gtagtggaac agctgtctcc acgagataag
    78481 taggggacat ctgttttaat aactaaagag agatgggaca tgtagtggca ctcaagaatt
    78541 agatttttca gtctaacttc gaggtactct accttcccat cctctctttt ttatttcttt
    78601 ttgcggatga ttccgtaagc tgctgcgact aacacacccg cccctgctaa ggaataactg
    78661 acagaagctg ttttttcatt tgtttttggc aaagtctgac ttgttgattt ggatggctct
    78721 gtcgtttgct gaactgtttc tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa
    78781 tgcaaaatat cttttattcg ttggaaacta gctgctaagt cagctttttt tgcttcaaaa
    78841 tcattagcaa ctgtttgtgc ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg
    78901 atatccgctt gagtttgact tagctttcca gaagctgttt gagcaacctg atttaattgc
    78961 tgattgaatt cttttgcttg ttggatcttc ccgtcaagat ctgtttggac agcttccgct
    79021 tttgctttcg cttcggcaag ctttttctca agttgtgcac ggtcatttcc cactttattt
    79081 ttaagatcag ctaaatcttg atcgattgct gcttggtctt ctttcaattt gttttttgca
    79141 gcttctaatg cttcttcgag ttcttttctt ttattttcca agtcttgcaa atgatcgatt
    79201 tggtcagaag aggtagaact atttattgat gtatcttctg aagatgaaat cgtttctcct
    79261 gaattatctg tcggtacatt ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca
    79321 gaagagctta tggttgttga aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct
    79381 gtctgtccca ataagaaaat actggaaaca gcaattggcc agattaacgg cttcattctt
    79441 tttttcatca cttttcccat ccttactact ttttaactaa tttcttatta taagcaaaaa
    79501 tgtttggaag aaccataaac acaacaggct gtttgtctaa aaaacggtgc atcttttatt
    79561 tttttgtacg ttccgggctt ttccatgtcc cgcttccata acctaaaatc gttttgactg
    79621 ctggaatgaa agtcgtcacg atcgttatcg cgttcactat ccagaacaaa agcatatata
    79681 gtggtgcgaa aagaaggtat ttcaattttc ttcgtcgatc atctacaatc agcgaagcta
    79741 gcaactggaa aaatcctgcg accatttcaa agcagatgaa cacaaaactg aatgtaaaag
    79801 taatataaat ctgttcgtag tttccttgat atacataatg cccgattaat ccagcaaaaa
    79861 gaactacgga aatacaaaag aatattgacc agacaatact caatgtttga tcaatgaaga
    79921 tgatcgttct tccaatatgt ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc
    79981 agacttctgt tccacctttt gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca
    80041 caggaacttc catgaaaaag ataatctcag gagcaaaaac agataaccag tcatttaact
    80101 gatgatccca agcaatactg atttcttctg tcgctcgatc ttgtctgaat ccgcccacat
    80161 cgatcaaggc ttcttttcga tacatcgtat ttgctccact gtacgcataa agaccaccga
    80221 aaacagctga ctgtgttcgt ttaatgatgc ccacgatact tgagaattca accgtttgag
    80281 attttgcaat caatttggta cgattttgga catccatatt tgcagtgacc gcagcaatat
    80341 gtcttgcacc agggcgaatg aaataattca catacctaat cagtgcatct ggctccggta
    80401 ccgtgtctgc atcattgctt aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa
    80461 acgcgtgggc ttttccttta tttttttcga tccgaaccac acgaagatta gcgtattttt
    80521 tcatcagtct agctaatatt tctggtgttt ggtccgttga accatcgtct gtcactaata
    80581 cctcatagtt gtgataattc aatttcgtca tcaaatactc gattgtatat tcgatcacaa
    80641 tttcttcatt atgggctggc accatgatcg tgatgaacgg ttcgacactc aagggaacat
    80701 ctacccattc tttttgctta tgtttgtata taaacacata acaccagaca ccaataaacc
    80761 atacaaatcc gcctaaaatc ggataagaaa cgagaaaaaa atacataata gacaaacaag
    80821 tatctaagaa taaattacct gtatgggtga ttgagatcat ttcagttcca ccccattttc
    80881 tttatacaaa tctgcaatga atgtcgtatc tagatttttc tcttcggaga cagaataaaa
    80941 acggaccgtt tctctctctt cttttgttcc aaaacgttcc gtatacactt cttcaagtaa
    81001 ttcttgtctt tttttcaatt tttcatcttc atattcaaaa gatttattta cttttttgta
    81061 tagatttcga ttgttccacc tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat
    81121 caatacaaaa aagccgatta agaaatacag cattttcttt tctgataaaa aagcgtacaa
    81181 ggaaaaatcc ttggctcttt ctggtctcgt taatgggaaa aagacccata taaaaggaag
    81241 aacaacgatc atccatccta aaagagccac taacgtttgc caaatcttta accagtaatt
    81301 accttttgaa aaatacttat ctttcaccaa aattgatgca acttcttctc ttgttacttt
    81361 tttcatatcc tcacctcacg tttttttgtt acgacgtagg agtttaccat acatttcatt
    81421 tcttttcaca taaagtgttc ctaaattttt cctaagaaaa agaacaaaac atttctcatc
    81481 agcaaatgaa aaatattttg ttctttttat cagttgttct ttttattttt tatctcttaa
    81541 ttatgttgct ctttccaata cgacaatgct tttcattcct ctagatatcg ccgtgtacat
    81601 ttgtctgcgt cttctttgct catctccatc gttttcaaat ggatataaga ttacatgatc
    81661 aaattccagt ccttttgcta gactcaaagg aatgatttgg cagttttcta atccatatga
    81721 atttttttta atcgattctt gtagcgtttg ggtttcctct tctgtttggg tgataactgc
    81781 cgctttttct ccttttagag aaaccaaaat ctcagctagt attttcaaca attcttcaga
    81841 ggatcgaaaa gagcggtatt cgggttcttt tccttgcggt cggatcgata cgatgtcgat
    81901 cttctcttga tccacagcat aggttttgaa aagctctgtg attgctccgc tagaacggta
    81961 actatttcta agatcatacc gatggatcgg cagatggtag tcgtcgaaac aacgcataat
    82021 atcagcaaaa gtagtggaag aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa
    82081 gtgagttttt ggaaataatt cgatcaatag acctaattgt gccagggtat aatcttgtac
    82141 ttcatcaatc aatagatagc gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg
    82201 aatagctaat ataatgactg cttcatcaac agtacgtgtg ggttgccaag cataagctga
    82261 gccatatcgt ttttcataaa ttctttcaaa caactgatgc tcatccaccc atgccatttc
    82321 ttcgatttgt ctggttattt ttcgatattt tttttgtaaa agtttccttg cataagctac
    82381 aatactttgt tccgaatcat cagaaatcaa tttaccgaat aattccaact gcctatcttc
    82441 tgtcaacgag agtacctgat tctgcacagc aggacttttt gcttgtttca aaagatgatt
    82501 cttccattcg ctcatcagtg cctttttcgt tgcttgaagc ctatcgatca atttaggatg
    82561 agcaggaact ttctcatata ttttttcgat cgttttttta gaaaggactg tttttccatt
    82621 tttagtaagt ccacggataa aattcggatg atctaaaaac aaaggatctg attgttttag
    82681 attatcgata aactttttgc tacgcaaacg ttctgtctgc tccgatacat tttctcccgt
    82741 gatacgtttg aaataagcct cttcaccttc tatttcctct gctgaaagct gtgaaaccag
    82801 ctgtatgatg gttagattca gtggatttct ttcacctaat gatggtagga cattcgaaat
    82861 atagtcgata aaccgatgat taggtgatag aatcagcaag tcatcggatg tcatcgtatc
    82921 tcgatataaa tagagcagat acgcaatacg ctgcatgata gcagaggttt taccacttcc
    82981 tgctacgcca tttaccaaga tattttgact tttcacatcc cgaatgattg tgttttgttc
    83041 cgattggatc gtagcagtga tagccttcat ttcattggtc tcatcttggc ctaagacttc
    83101 taataatacg tcatcttgaa tagctacaga cgtatcaaaa aacttgacca aacgatctct
    83161 ttccaaaaga aattgtcgtc tttctttaat aaccgtatcg attttatttt gacgaaccgt
    83221 ataagaagat ggaccaatca tgttattgta aaaaagttcg gcaattggtg aacgccaatc
    83281 ataaacaaga ttttcatctg catcatctgt gaaatttgct gtaccgatat aaaaggattc
    83341 ttttggctca ttatccagaa agtctacaac gacttttcca aagtagggac ttttcaataa
    83401 acgttcgatc tttgctagct gattttctgc gtttttctgt ttgatattca tctgatcgat
    83461 ttctctattt ttcatttcaa tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa
    83521 attcaatcga agctcgccag ccatttcttt caacgactgg tttccttcta tttttgccgt
    83581 atccatcgat tcccgcagtt cgtttttttt atcaattagc ttttgataca cgttcttcag
    83641 atatgcttct tctaactgtt tttgtgtgtt gaccatacac attctcctct ttttttaaag
    83701 tggtggaacc gattagttta cctcttttaa gagtcgaatg caagcttttt aacgagtatc
    83761 atttgttagg atatatcagt tcttcaggtc ggacagtttg atataaagaa aatgcaccat
    83821 caaggttttt tacgtggtag ccagactgct tcaagattct ttccgctaga tagctacgta
    83881 atccgctatg acagctgacg atatattctt gttggctgtc taattcattt aatcggtccc
    83941 gaagttcatt caaagggata tgtttggctt ccttgaattt gcccttttcc aattcttctg
    84001 ctgttcgcac atctaataaa atttttcctt ttgctagttc tgtcggcaat tcatgccatt
    84061 gtacggtttc actaagccct tctgcaatgt tcattgctgc atatcctagc atgtttactg
    84121 gatctttggc tgagccaaat ggcggtgcat acgtcaattc taattccggt aaatcgaaga
    84181 tcgtcaaatg ccctttgatt gctgttgcta aaatatcgat ccgtttatcc acaccttttg
    84241 ccccaacacc ttgtgcacca taaatctcgc cagtctctgg atggaagatc aacttcagta
    84301 aaatatctgt tgcttctgga taatagcttg catgatcttt tcctgataca tgaatgactt
    84361 caaaagacag ttcgttttct ttggctgttc tttcacttaa accggtggat gcggcagtca
    84421 ttccaaacgt tcgtataatt gctgttccaa taccgccttg atttcttcga gaaattccgg
    84481 aaatcgtgtc cgcaacttgt ctaccttgac gattggctgg tgaagcaaga gaaataagtg
    84541 catcttttcc agtgatttgc tgttttacaa cgatagcatc ccccaccgca taaatatcag
    84601 gaatattcgt ttgataacgt tcgtctacta ggataccccc gcgtatgcct aattcgattc
    84661 cagcggtcac tgctagctgg ttatcaggac gaacaccaat ggataaaata actaaatcag
    84721 cagggatggt acgcccatca tccagtcgca atcgtttccc gtggtcttca aatccagcga
    84781 cagcatgaga agtaatcact tctactcctt gatgagacaa ttctttttct ataaaagctg
    84841 ccatttcttc atccagtgtt ggaagaatat gtggtgcttg ttcgatcacc atgacttcta
    84901 aacctcttct ttttaggttt tctgccattt ccaatccgat gaatcctgca ccaacgatca
    84961 cggcacgctt tggctgtttt tctaatgcat gcatcacttt atcgatatct ggtacatttc
    85021 taatagaaaa aacattgtcg gcttctgtta tccctggaat cgatggaaca actggttttg
    85081 caccaggaga taaaaccaat gcatcgtact gttcaatgtg tttttgaccc tcatgtacga
    85141 cttctaccgt tttgttttcg ggaaagatgg ctgtcacttc atgatttgga cgcacatcta
    85201 aacaaaaacg agcggataat gattctggcg tttggacaag aagattttcc cgttcactga
    85261 tttctcccga aagataataa ggcaagccgc aatttgcaaa agaaacatac ggtccttttt
    85321 caaaaacaac gatttctgca tcttccatta atcggcgaag ccgtgtagca gcagacattc
    85381 cacctgctac acctccgaca ataacgattt tcatcttatt tccctcctac tgttcgatca
    85441 aaccattgat tcatgccacc acgtacattc actacatcat aaccagattt ctttaattct
    85501 ttagctgctt gtttactcct catccctgat tgacaaatca catagacggt cttttctttg
    85561 tctccatcat atctgtttat actttgtaat ggaacatttt tagctccttt tatatgcccg
    85621 ccccggtatt ctgcaggtgt tctaacatca agtaacgctg gattttccaa aagcttttcc
    85681 tttaattctg ggactgttat cgattttatt gattgaaata gcaaattcat tttctgcacc
    85741 tcatttacat ttttataaat acctcatggg gtatattaaa caggagcttt attaatatgt
    85801 caaccagaat gactcttcat ttttttcact actttttata tttgcataaa ataccgataa
    85861 ggggtattat ctaattataa aaatagagga gaatgtgtta tggagattga taataaaaat
    85921 atcctgaacc gtttgaaacg tacagaggga caaattcgtg gtatccaaaa aatgatcgaa
    85981 gacgaaaaag aatgtatgga tgtcatcact caattaagcg ctgttcgttc tagtattgat
    86041 cgcgtaatgg gcatgatcgt tgcagataat ctgaaaaatt gctttgagaa acctgatagt
    86101 aatccagaag aacaagcggc taaactagaa caagcgatca agatgattat taaaaagtaa
    86161 aggaatgatt tcaatgaaag tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac
    86221 agggaaaaac gtaaaaaacc tgataaaagc ttctccagaa acgacgattg tcgtatctgt
    86281 aaatggcatc gcgattaccg gttatctaga ctctgccaat gcagaattct tagatttgca
    86341 aggagtcgtt tttcacgctt gcgccaatgc tatgcgtgct aatcatattt cagaaagttc
    86401 tttacctgaa caagtaatcg ttgtaccagc aggtgtcctt gatttagttg aactgcaatc
    86461 ccaaggttat gcttatatca agccttagtc agtagaggtt gcgacagaag tgatcagctt
    86521 caagaaattt cggcttattt ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt
    86581 gtgaaaacgc tgtcctgcat caagtaataa gaacagccaa acaaaaatag cttctcatat
    86641 ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga
    86701 ggttgtgaaa tcagcggttt gacctaagta ttagagaaaa aaacgggaaa atagctcctc
    86761 atattttgcc gttttttgag ctaataccga aggtcagctg attgaacacc gtttaccaag
    86821 aggttgtgag cctaccattt aactccgaat cacaagaaat aatttcaaaa acaacttctc
    86881 ctgtttttgg tttggttggc ttattatcga gaagtagcta ctgggagtct atttatcaaa
    86941 aaaaggaatg cgacataggt cacacccctt ttt
//
LOCUS       DAFEPQ010000004        85312 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.004, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000004
VERSION     DAFEPQ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 85312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.004"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(95..523)
                     /locus_tag="LP543_000279"
     CDS             complement(95..523)
                     /locus_tag="LP543_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320475.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(551..1189)
                     /locus_tag="LP543_000280"
     CDS             complement(551..1189)
                     /locus_tag="LP543_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2320476.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1193..3175)
                     /locus_tag="LP543_000281"
     CDS             complement(1193..3175)
                     /locus_tag="LP543_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBL2320477.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3355..4020
                     /locus_tag="LP543_000282"
     CDS             3355..4020
                     /locus_tag="LP543_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2320478.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(5955..9104)
                     /locus_tag="LP543_000283"
     CDS             complement(5955..9104)
                     /locus_tag="LP543_000283"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HBL2320479.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(9117..10316)
                     /locus_tag="LP543_000284"
     CDS             complement(9117..10316)
                     /locus_tag="LP543_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBL2320480.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(10306..11901)
                     /locus_tag="LP543_000285"
     CDS             complement(10306..11901)
                     /locus_tag="LP543_000285"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBL2320481.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(12485..13192)
                     /locus_tag="LP543_000286"
     CDS             complement(12485..13192)
                     /locus_tag="LP543_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320482.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(13257..14093)
                     /locus_tag="LP543_000287"
     CDS             complement(13257..14093)
                     /locus_tag="LP543_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL2320483.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(14111..15520)
                     /locus_tag="LP543_000288"
     CDS             complement(14111..15520)
                     /locus_tag="LP543_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL2320484.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(15534..17357)
                     /locus_tag="LP543_000289"
     CDS             complement(15534..17357)
                     /locus_tag="LP543_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL2320485.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(18200..18526)
                     /locus_tag="LP543_000290"
     CDS             complement(18200..18526)
                     /locus_tag="LP543_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320486.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(18572..18934)
                     /locus_tag="LP543_000291"
     CDS             complement(18572..18934)
                     /locus_tag="LP543_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBL2320487.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(18971..19186)
                     /locus_tag="LP543_000292"
     CDS             complement(18971..19186)
                     /locus_tag="LP543_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2320488.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            19313..19648
                     /locus_tag="LP543_000293"
     CDS             19313..19648
                     /locus_tag="LP543_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2320489.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(19724..21721)
                     /locus_tag="LP543_000294"
     CDS             complement(19724..21721)
                     /locus_tag="LP543_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320490.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(21819..22094)
                     /locus_tag="LP543_000295"
     CDS             complement(21819..22094)
                     /locus_tag="LP543_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2320491.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            22326..23060
                     /locus_tag="LP543_000296"
     CDS             22326..23060
                     /locus_tag="LP543_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2320492.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            23099..24241
                     /locus_tag="LP543_000297"
     CDS             23099..24241
                     /locus_tag="LP543_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL2320493.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(24336..25727)
                     /gene="sufB"
                     /locus_tag="LP543_000298"
     CDS             complement(24336..25727)
                     /gene="sufB"
                     /locus_tag="LP543_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBL2320494.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(25732..26202)
                     /locus_tag="LP543_000299"
     CDS             complement(25732..26202)
                     /locus_tag="LP543_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBL2320495.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(26189..27424)
                     /locus_tag="LP543_000300"
     CDS             complement(26189..27424)
                     /locus_tag="LP543_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL2320496.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(27421..28707)
                     /gene="sufD"
                     /locus_tag="LP543_000301"
     CDS             complement(27421..28707)
                     /gene="sufD"
                     /locus_tag="LP543_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBL2320497.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(28720..29493)
                     /gene="sufC"
                     /locus_tag="LP543_000302"
     CDS             complement(28720..29493)
                     /gene="sufC"
                     /locus_tag="LP543_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBL2320498.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(29690..30538)
                     /locus_tag="LP543_000303"
     CDS             complement(29690..30538)
                     /locus_tag="LP543_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBL2320499.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(30562..31248)
                     /locus_tag="LP543_000304"
     CDS             complement(30562..31248)
                     /locus_tag="LP543_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2320500.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(31241..32275)
                     /locus_tag="LP543_000305"
     CDS             complement(31241..32275)
                     /locus_tag="LP543_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2320501.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(32338..32594)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(32670..33008)
                     /locus_tag="LP543_000306"
     CDS             complement(32670..33008)
                     /locus_tag="LP543_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBL2320502.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(33010..33366)
                     /locus_tag="LP543_000307"
     CDS             complement(33010..33366)
                     /locus_tag="LP543_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBL2320503.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(33372..34559)
                     /locus_tag="LP543_000308"
     CDS             complement(33372..34559)
                     /locus_tag="LP543_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL2320504.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPVRS
                     SRR"
     gene            complement(34750..35595)
                     /locus_tag="LP543_000309"
     CDS             complement(34750..35595)
                     /locus_tag="LP543_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL2320505.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            35829..36794
                     /locus_tag="LP543_000310"
     CDS             35829..36794
                     /locus_tag="LP543_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBL2320506.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            37171..38214
                     /locus_tag="LP543_000311"
     CDS             37171..38214
                     /locus_tag="LP543_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBL2320507.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(38276..38740)
                     /locus_tag="LP543_000312"
     CDS             complement(38276..38740)
                     /locus_tag="LP543_000312"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBL2320508.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            38986..39933
                     /locus_tag="LP543_000313"
     CDS             38986..39933
                     /locus_tag="LP543_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL2320509.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(40144..42216)
                     /locus_tag="LP543_000314"
     CDS             complement(40144..42216)
                     /locus_tag="LP543_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL2320510.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(42149..42526)
                     /locus_tag="LP543_000315"
     CDS             complement(42149..42526)
                     /locus_tag="LP543_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBL2320511.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(42655..43233)
                     /locus_tag="LP543_000316"
     CDS             complement(42655..43233)
                     /locus_tag="LP543_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL2320512.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            43365..44657
                     /locus_tag="LP543_000317"
     CDS             43365..44657
                     /locus_tag="LP543_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL2320513.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(44717..45835)
                     /locus_tag="LP543_000318"
     CDS             complement(44717..45835)
                     /locus_tag="LP543_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBL2320514.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(45849..47270)
                     /gene="arcD"
                     /locus_tag="LP543_000319"
     CDS             complement(45849..47270)
                     /gene="arcD"
                     /locus_tag="LP543_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBL2320515.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(47516..48637)
                     /gene="mnmA"
                     /locus_tag="LP543_000320"
     CDS             complement(47516..48637)
                     /gene="mnmA"
                     /locus_tag="LP543_000320"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBL2320516.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNDKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            49041..49640
                     /locus_tag="LP543_000321"
     CDS             49041..49640
                     /locus_tag="LP543_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HBL2320517.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(49778..51064)
                     /gene="efmA"
                     /locus_tag="LP543_000322"
     CDS             complement(49778..51064)
                     /gene="efmA"
                     /locus_tag="LP543_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HBL2320518.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(51335..51679)
                     /locus_tag="LP543_000323"
     CDS             complement(51335..51679)
                     /locus_tag="LP543_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBL2320519.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(51816..52961)
                     /locus_tag="LP543_000324"
     CDS             complement(51816..52961)
                     /locus_tag="LP543_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL2320520.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(53141..54115)
                     /locus_tag="LP543_000325"
     CDS             complement(53141..54115)
                     /locus_tag="LP543_000325"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBL2320521.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(54195..55070)
                     /locus_tag="LP543_000326"
     CDS             complement(54195..55070)
                     /locus_tag="LP543_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBL2320522.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSEKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            55200..57107
                     /locus_tag="LP543_000327"
     CDS             55200..57107
                     /locus_tag="LP543_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBL2320523.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(57648..60926)
                     /locus_tag="LP543_000328"
     CDS             complement(57648..60926)
                     /locus_tag="LP543_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBL2320524.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(61197..61742)
                     /locus_tag="LP543_000329"
     CDS             complement(61197..61742)
                     /locus_tag="LP543_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL2320525.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(61754..62446)
                     /locus_tag="LP543_000330"
     CDS             complement(61754..62446)
                     /locus_tag="LP543_000330"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBL2320526.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(62465..62749)
                     /locus_tag="LP543_000331"
     CDS             complement(62465..62749)
                     /locus_tag="LP543_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320527.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(62762..63325)
                     /locus_tag="LP543_000332"
     CDS             complement(62762..63325)
                     /locus_tag="LP543_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL2320528.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            63553..64215
                     /locus_tag="LP543_000333"
     CDS             63553..64215
                     /locus_tag="LP543_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBL2320529.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            64339..65535
                     /locus_tag="LP543_000334"
     CDS             64339..65535
                     /locus_tag="LP543_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBL2320530.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(65636..67012)
                     /locus_tag="LP543_000335"
     CDS             complement(65636..67012)
                     /locus_tag="LP543_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2320531.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(67049..67915)
                     /locus_tag="LP543_000336"
     CDS             complement(67049..67915)
                     /locus_tag="LP543_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HBL2320532.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(68004..69029)
                     /locus_tag="LP543_000337"
     CDS             complement(68004..69029)
                     /locus_tag="LP543_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL2320533.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(69047..69721)
                     /locus_tag="LP543_000338"
     CDS             complement(69047..69721)
                     /locus_tag="LP543_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2320534.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(69905..70798)
                     /locus_tag="LP543_000339"
     CDS             complement(69905..70798)
                     /locus_tag="LP543_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL2320535.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(70872..72569)
                     /locus_tag="LP543_000340"
     CDS             complement(70872..72569)
                     /locus_tag="LP543_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL2320536.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(72562..73875)
                     /locus_tag="LP543_000341"
     CDS             complement(72562..73875)
                     /locus_tag="LP543_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBL2320537.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(73875..77393)
                     /locus_tag="LP543_000342"
     CDS             complement(73875..77393)
                     /locus_tag="LP543_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBL2320538.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAISDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(77686..78144)
                     /locus_tag="LP543_000343"
     CDS             complement(77686..78144)
                     /locus_tag="LP543_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL2320539.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(78442..78960)
                     /locus_tag="LP543_000344"
     CDS             complement(78442..78960)
                     /locus_tag="LP543_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBL2320540.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(78962..79579)
                     /locus_tag="LP543_000345"
     CDS             complement(78962..79579)
                     /locus_tag="LP543_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBL2320541.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(79576..80430)
                     /locus_tag="LP543_000346"
     CDS             complement(79576..80430)
                     /locus_tag="LP543_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2320542.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIILLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(80427..81338)
                     /locus_tag="LP543_000347"
     CDS             complement(80427..81338)
                     /locus_tag="LP543_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2320543.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(81379..82713)
                     /locus_tag="LP543_000348"
     CDS             complement(81379..82713)
                     /locus_tag="LP543_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL2320544.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            82921..83751
                     /locus_tag="LP543_000349"
     CDS             82921..83751
                     /locus_tag="LP543_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBL2320545.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(83995..84313)
                     /locus_tag="LP543_000350"
                     /pseudo
     CDS             complement(83995..84313)
                     /locus_tag="LP543_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(84435..84629)
                     /locus_tag="LP543_000351"
     CDS             complement(84435..84629)
                     /locus_tag="LP543_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320546.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT        24926 a        17907 c        14717 g        27762 t
ORIGIN      
        1 tacaactaaa aatgaatata ttaaaactac caaaaataac ttttaacgct gtttcattat
       61 atgttttttc tattttatat aaacttttgg atacttaata ccaaccggtt ggattgtacc
      121 aacatgagaa tgtgtcagtt ctatttcctg tcgcacttgc ataggcccat actccttttg
      181 ttgcatttgc tgatcgaata gagtttccaa ctttcgcttt tgcaccgtga cgattataga
      241 cacgactata gtggttagaa tgacctgttt ttctcgtaca agttgtatca ctataataaa
      301 cccatcttcc agagctatga ttaattgctg ttctagaaat ttcccggtct actggtaaat
      361 ttgacttaat aatattacct ttagcatcaa aatgaatctc tcctggcttt aaagatttgt
      421 aatagatttc accgtgaact gaatttgctt ccacttcaga tgacatagcg tttcctccaa
      481 caaaacctat ccctagtgca caaataataa ctgctttttt cataaaacga catcctttcc
      541 tttttgattt ttaaagcatg atcttcttgt ttcctatttt ggtcagatat ggatcatgcg
      601 tgactacgat aactaacgta ccttgggaag cttgttgcga taagatttca actactttat
      661 ccttatttct tttatccaat gacccagtcg gttcatcggc taaaatgatt ttgctattct
      721 tcaataagac tctcgcacag gcgattcttt gcttctcacc accagataat gtgtagatag
      781 gcgtacttag cttcttgttt atatttactt ttttcaatgc ctcatacttt gctgttcgct
      841 tatctgaaac atttaaattc gaaaacttca atccaatatc taaattaaaa tcgattgttt
      901 catattcaat caacccaaaa tcttgaaaaa gaaaagatat ggtatttctt tttatcaagc
      961 gcttctctct cccagttaat tgtttcgtct cttgatcgaa caaatagaat tgtcctgaac
     1021 tgaactgatc attcaatcct aatatattca ataacgtact tttacctgct ccactttctc
     1081 caacaacagt cacaagatcc ccagcatccg cagtgaaacg atccaaatga agaatcgttt
     1141 tgtttcgaat gatttttttc gcacctttta tcactatcaa cggctccaat tgttaacctc
     1201 cattcatcag caataactga cttttctttt ctgtagacaa aataaaaaca actgaaagaa
     1261 taaaatcaat gaagctaaag aaaaaaatat taaaaagata tttaggctct ctttctataa
     1321 gaaaaaggaa gattacactt gtcgttcccc atttgatgag gttcatcatg aagaaatctt
     1381 tatacgtttc aaagagcgaa taaccacttg aacgaaagat agcgattttc cgacaattgt
     1441 agacaaaata tatgcataac atgacatagc tcaacaagaa gaaagaaaag aaagtgagta
     1501 aaaatatctg tatcaagtaa tgaaatgatc tgcttaacac tttgacagct atctcttcag
     1561 tctgagtata cggaaatgat aactgcaagt tatcacgtaa ctgactttct tcaatcagct
     1621 caaataacct ttctttcgac aaagagccta tcttgatctt taacggtgga tagatttctc
     1681 cgctgagaat atttctttcc aaatacatac tgtttttcaa tgtcagtatt tcaacaaccg
     1741 gataatgttc gatccagggg atagaaggaa taaatgtata aatgggttga ttgtttttta
     1801 taaatagaaa tttcggttct ttttcactga tttcttgata atattctgtt atttgcgcca
     1861 ggtcattttt taaatctgta tgtctatatc gttcagggat caatataaca gggtgcgttt
     1921 ctttttcggt tatttggatc aaccttccag agacatcaat caaaggaaat ttcttaagat
     1981 aattcagatt tatttttatc cctctgccaa actgttcatt ttcttcattc aagtaatctg
     2041 atacattgac taatatactt ccgtttttat tcaaagtttc atataattcc tgatctgctg
     2101 ctgatgcaaa acgattggaa taaataaaat ccacgtggtt tttcccgatg gttactggat
     2161 aaaatacatg atagtcctca ccagcgattt cttttggtgg ttcatgggaa acagctaact
     2221 gtgaagaaga aaaaacgata gttgctaatt cgacattggc aacggtcaaa agaagagaaa
     2281 acaacaatcc aaatgtaaaa agaatgtatt ggatatagtt gaacgcttct cttaacgaag
     2341 cgattttttc aactaataaa agagaaatca aagagatacc atatattccg ctgaacaaga
     2401 aaacgtacat ctggaacaaa taaagaaatt gctccttata atacataaaa ataaaaccac
     2461 caatgattcc tgcaattatg aatgtaataa tgattttctt tttccccaat aaacaccaca
     2521 atacttgatg taatgaaaag tcattattca acaataaact gatttcttta ttcatcaata
     2581 agaaaaagga tgataacaaa aggaataaaa gaatcaatcc aatagttaaa tacactcgat
     2641 aattatatac gtctgtattt gaaataaccg aatacgaata gctattttca aagtcttcaa
     2701 aaatattagt ggcataatca gtgtgaaatg cttgattgaa tcgatcagtg aatagtgaaa
     2761 tgacccgttc ataggcttct ttttctctcg tcatcaagaa aaattcacct tcaaaatctt
     2821 ttattttgat cgattcttca atggacgtta cgcgaaagga ggtattagaa tagggtacct
     2881 tctcgtgaga agatttgaca tccccttgat aatacaatgt catttcaagc tttggctgta
     2941 tcttgaagtt ccaagatgta tctacttcat tggattcatt tactgttttt ttcatataag
     3001 aaagctcagt ttctgatgag acattttcaa ataccatgat tagctgctca tattcttctt
     3061 gcccaataaa atgttgtggc aatcgaatcg gagtataagt atctgtttga tggaaaaatt
     3121 catttgtccg atagagtacc tgtttattta tactaaccaa ggcaaaacca ctcatgatac
     3181 cggtcactat gattgtgagt aaaactgtga actttttttt gattatcacc actctccctt
     3241 acttaaatga ttctttgata aaatcatacc gtttttcatc aaaaaaacgt atctatcaag
     3301 tgtataggtc atgtagaata aatctatata ctgtagaatc gaaaggagaa gaagatgaac
     3361 accggagaaa aaatacgaac ttaccgcaga aattgtaagc ttacgcaaaa agaattaggc
     3421 gaaaaaattg gtgtttccga taaaacgatt tctagttggg aaaattctcg tacaatgcca
     3481 gactttgaaa tgctgtcact tttacatcat actttaggtc tgccgattga ttttccttct
     3541 attgaatcag ctaccgaatc aatagaacca acattgccag atgaacctct tgttaacaag
     3601 aaatcttatt catcaaaaca acccttctca ctcaaaaaga cactatcttt ctttctgctc
     3661 gtcttttgtt tggtctatct ttttcttatc aattatcaat acaaaaatga aacgatcgat
     3721 cgattcaatc cttttttgaa accacaaatc ggctatacca cactccctgt aggatacgtg
     3781 gacaattcca tcgaacgttg ggtatgtaat gatatctttg gccatggtac atggctgtct
     3841 tttcacgctg gagaggtaga aaaagagaaa aaccatgctg taatcaaaca caaaggaaaa
     3901 cacgtcatga acgttcgcct gctcacttgg gaagccattc cctcagatta ccaagaaatc
     3961 ctcagccaag actatgacga cgccttaaaa agaagccctc aggagccaga taggcattaa
     4021 gtaggagatg aacgtagaga tagaaaaaat caatgcacat gatcttatcc aaaaaacagg
     4081 ggtacaaatt aattgaaaaa gccttgagat gaaagttcct aaataaaaaa gttcagaatg
     4141 ataggtattt ctggcttata gtataatatt tacgccacag cctcccaatg attctttttg
     4201 gaatcataat caaggatgga cattcacaaa aatattttgc tcattcgatc cgaatagatt
     4261 aacgaccaat ctagtaagta atatagattg gtacgtagtc aagctgcaca taatttagat
     4321 caattttagt tcttttttat atttctatga tctgctcatt tacttttata tgatggctta
     4381 cagtaacata aataaatatg ataatcgctg gtagtttatc caagagtact caataaaata
     4441 caaaatggat tgttcaagcg cctatcataa tgataaaatg aaacaaataa acgatacaag
     4501 gaggaagaga ctttgagtaa tggcagaaca attttttcat tgtaaaaatt gttcagttgt
     4561 taatctatgg gatttcggta gcatgggcat ttttttatag aggaatcaca ccaacctggc
     4621 cgttttatct cttgttagtg ggaatattag taggaaattt tgcccctctt cactatcgta
     4681 cgaacttttt tccaactgat tctggccttt ttcttaaaac aaagacgcga aaattagaaa
     4741 acattattga aatgacaatt acctgtataa tcattttctt agttatattc ctaaacgtct
     4801 tctaataaga atctcttaaa attagcttgt tacagttagt tacctagttt atataacaca
     4861 gttatcttat cttccttatc aacatctttt gccaactctt tgtcttaaat tcgaaacata
     4921 taagcagagc tacgaacacc aagactgttt cggctctgtt ttttgcatca aattcactct
     4981 tatatagctg ataatctcgg ctctttaaaa gaaattattt tcgcttcatt ttattataaa
     5041 aaggctagga accttgatgt tatcaggttc ctagccttta tttaagtatt accccacaga
     5101 cccttccata tagattttta aattacacac tagtttaaac taaaacaaac aaggaaaaac
     5161 ttaccattta atcaaaaaat tagctcatat gaatatatta gaatgtaaat tagactggtc
     5221 aaaatctggt caaaaaagag gacgaataca ggtagtcctc ttttttgtta ttttgatatt
     5281 ctaaatcatt ttttattgga ttattccctt gttcgatata gacccaaatt gatctatcaa
     5341 taattatcaa actgagtcat atagagacaa aaatttcaaa aatgacctgt taaatggtca
     5401 tcgaatatca tttaaaatca atacgacgga gtcagacacg gagtcaaaac aagcccgctg
     5461 agagtaaaaa atatacagtc ccataggtaa agtgccacag gactgtatat ttttttattt
     5521 gagattacct gaaatgattc catttaattt ttatctcatt ttttcctact agttagtatc
     5581 aaatactctc ctattttctc aataaactat tttgggttac atggtatctg aaacagctaa
     5641 aaccttgtca ttatagcaaa aaatcacttt atctccgttt catttcagat acatttcact
     5701 taagcggatt ccaaaaaaac aaaagaaaaa ggaaccccta gttagcctat gctttttatt
     5761 ggttcctctc aaaacacggt catataacaa cggggtgggg taaatgaatg aaaaaggtgg
     5821 aggaaacatt attcaatagc tgtcttaaat acttcgttat aaaatgtgaa caaataaaag
     5881 acatctatcc ctattttcta cacttccact tgactaccat ctttgaatcg tacaattttc
     5941 ttaactcttc tcgtttacct caattcattc tcaagtggaa tcaactcatc atctaacatt
     6001 ttacgccatg ctcgcttcat ttcttgtggg tatttaaatg gcttcgcttc tggatgtttg
     6061 gctttatatc cttcgtaatc cttactgttg ataattgctt tcatattagg aattggatct
     6121 tcacctggta gatattgcag attagaagca tgtaattctt tctcagaaac aaaccactca
     6181 ttggaaaaga catgaatcaa atcatcactc gcttgtgtaa aatagcgacg tttaatgaca
     6241 aagatatctt cattactatc taaagtcccc acatcaaatg catttaaaat ttgtcggtaa
     6301 acttctttga ctccttctgt tttattcaat ttagccaaag ctttttcaat ttcttctcgt
     6361 gcatctgaac tattcgcagc ataagttcct agtaattgat caatataagt tgaatcaata
     6421 tcatacaact tagtggtatt ttcactataa aattcgatcg ttgaaaaatc ttctccttct
     6481 ttttctggtt cttcctctat taatgaaccc ttcacggtct cgtacacgcc aatttgttct
     6541 tccaaaagtt gaatttgatt gttcaaagtt ggagatttca ccatgtcatc attgtaatcg
     6601 tcataagtca ctaacgattc ataagcacga ttcaattctt gaaatgcttt gacgtactcg
     6661 atacggattt caagtggcgt atgttcatca atggattcca tggatgaaac ctcttttaac
     6721 ttaccatatg cttgtttgaa tctttttttg gattcctgat aacttggata aatcaaacct
     6781 gattcttctt ttgcttttga atacagtttt gtcgcatcct cgacattctt ctccattgtc
     6841 gctggcttac gaaacgtcac aatcaatcct tttgtcttat caggatacgt acgattcgta
     6901 cgtgaaaaag cttggatcaa tcctgcatac tctagattac gatccacaaa tagggtttga
     6961 atcgtagggg cgtcaaaacc tgttaataga cgatccacta caatcgctaa atcgatttgt
     7021 ttcccaaatt gtttgaattc cgctcttttt cgtgccaaac gattattgat gtctccgtta
     7081 taacgctcaa tgtctgctaa tgaccaggaa gtatcataat actgattata ttcctcaata
     7141 attttttgca tttcttcttg ttgggcactt gaatctcggc tattttcttc taatgaatag
     7201 gtaatagcga ttcgtggaaa atcaggatcc tccatcgttc gtccttcacg aatgagctga
     7261 ttgggaaact cttttgttaa ccaatcaggc tctttcgtca tctctttgat ggcccgataa
     7321 tactttttgg ccatgtcaat ggaactcgtg gttaagattg cagatttcgt tggcctgcca
     7381 tttcgaaaat caaattttgt ataggcatta tctggacgga acattttatg aatgactttt
     7441 tggatatgtt catctgcttc aaaaacagct gggtccaaat aactttcttt tttgacaggt
     7501 tccatttgat caatcatacg attgatctgt tctgatgaat actctgcata tgtttctact
     7561 tcttttaatt tccgatagat tttattctct agtgaagtcg gctccacggt gttttcatgc
     7621 tctacttgaa atcctaagac agagccatct tctagcgcat tcttaatggt ataggtatgc
     7681 aagacctctc catactgatc atgggttgta cgtgccaatt gccctttggc ttgtttccga
     7741 ttctcaggaa aaatcggtgt acctgtaaag ccaaaccaag tggatttcgg aaacattttt
     7801 ttgatttctt ccatattttc agcacttaac gctcgatgac actcatctac tacaaacacg
     7861 atatgttggc ccattaattt ttggaagcga ttggttcctt ttttctcttc ttgtttcttt
     7921 gcatatttca acgccgcatc tagtttttga cgggttgtaa tgattacaac atttgcattg
     7981 gcatctgcca acagggtttc acttaattct ttggcacttc ccgttccaac gatcaagcta
     8041 ttcgctttgg cgttcccgga agaaatccca gtattaaatt ccgaagcaaa ttttgtaaac
     8101 tctgtggtgg tttgattatc taaatctttg cggtccacta acatgatcgt tcgatcgatc
     8161 cccgattttc gtgctaaaag cttagtagat acaaaactag tcaacgtctt tcctgaacca
     8221 gtcgcatgcc acacataccc tgattgatgt ttatttgcgg caataaacaa ggcttgaatc
     8281 gcatgtactt gataaggatg taacaccatg agggttttat tatcttgatc ttcactgacg
     8341 attgtataat ccgcaatcaa acgatgagca tccgggatat ttaacacttg tttacaaaac
     8401 tcatataagt tttctacttt ttcattgtct ttcgttcgcc aactaaataa aaacttaggg
     8461 tgcaagtctt tcggtaaggc atttgcaaaa taccgagtgg tttgttcatt ggaaaccaca
     8521 aacaactgta aggtcgaaaa gatattgttc cgaaacatac cttcttctgc atattttttg
     8581 atttggttaa atgcctgata aacaccatct tttgcagtta cttgtttcag ctcgatttgg
     8641 acaatcggta atccattgat taataaggtg acgtcaaaac gtcgatcccg tgcttctatg
     8701 ttacttccac gttttgcaat ttgatggacc acttcataag tagagattcc tccaccaatg
     8761 tcttgattgg aataaaggat aagtgagaca gaacccaatt gcgaatcttc ccgttcaatc
     8821 gtgatccgag ccatcccatt ttcacctttt aaccatttcg cagcatcaaa tggggttttc
     8881 gttcgcagca gtaattccgt tttgatcgtt tcaaattctt tatcggtcaa aggaaaatca
     8941 ttcagttctg cttgattatt tgagataatt ttttggcgta aattgaccca aaggtcttct
     9001 tctgatttta gatccggacg atacgtccac tgattatgcc cttctccaag tacacgaatc
     9061 aactgttctt cgacttctgc ttcctcacgg tgactaattt tcattcttct tccctcctag
     9121 acaaacatct tttgtaagaa gctttttttc gtttctttta gtaaatctaa cttacgttga
     9181 tgaagagcga tcgtatcatc tagctgtttg aagaaagtac cgattttttg ttgttcatcc
     9241 tttacaggaa ccttaacact gatattctca atatcagaac tgttataaga aggcaatgcc
     9301 cctacttggg agtatcttgg caaatttata gtttcaaaca atattttacc aaaatagata
     9361 tcccaagtat tattaaataa ataagccatg gtattgttat ctattaagaa agggcttgtg
     9421 accaaacgtt ttctattaag cattattgct gcgccaactt tagcaaatat aatcgctggc
     9481 acatctttta ttggcttcca ttttttacgc tcaatttgct cattagatac ataatgattt
     9541 gcatttctca tttcatactc attaccaatg ttattcatgt cggaaacttt aaaaaatgga
     9601 gtaccttcag aaccaccttg ctcggcatca ggaaatccaa ttcccgattg agttttacct
     9661 aattctccca acttacgctc ttcccaatct tccgtaaaac caggaaaccg gatttctggc
     9721 acttttgctc cgttttcagg gaacattttt tgtaagaatc cttttttcgt ttccttcagc
     9781 aaatctaact tacgttgatg aagagcgata gtgtcgtcga gttttttgaa gaatatgccg
     9841 attttttgtt gttcttttaa atcattggga atatagaata ctgtatctga aacttttttc
     9901 cagtctgaac gaggcatttt tgttccagat gtatcatttg cagccttttg atatctatca
     9961 gcttgaataa gagaataaat aaagtccgag tcgctcttct tagctctaaa aacccagaaa
    10021 tccccaatcg ctacgcctgt aaaagttgcc ttgagccaat tttttaaata tggacgtaac
    10081 tttccatata gaatatcatt tggtaaaaat tcaatccctt ttctattatc aaatttctgg
    10141 ctcacatctt tattcagtct accttctcct gccacgatat cttcaaattc aacttttgga
    10201 agtctactgg agtttgtact tttattaagt ctaacaacta aatcaattaa cttacgctct
    10261 tcccaatctt ccgtaaatcc tgggaaacgg atttctggtc gtgtatcatt gctcatgttt
    10321 gaacacctcc agcgcacctt gtaaccacat ttcgttttca ggtgttgttt gcagggaaga
    10381 gatagcttct aaaagttctt tttctagcac ttgctgttct tgtcgtatct tcttgatgtc
    10441 atttccgaca tgaaccatat caattggttc ttcttcttcg aaagtatcta catagcgtgg
    10501 gatatttaag ttatagtcat tttctttgat ttcatcaaag gttgccaaat gagcgtattt
    10561 ctcgacatct tttctttcag cgtaggtttc gaggattttt tgaatgtttt cttccgatag
    10621 tttattttga tttttccctt taacaaattc acgactagca tcaatgaata atacatctcg
    10681 agtttgacga ttcttcttca acacaatcac cgtcgttgga atcgatgtcc caaagaacaa
    10741 gttcgcaggc ataccgatca cagcataaat actgccatct tctagtaatt tttgacggat
    10801 gaccccttct gcagcgccac ggaataaaac tccgtgtggt aagacgatgg ccatggtacc
    10861 tgtttccttc aaatgataga acccatgaag aagaaacgca aaatctgctt tggattttgg
    10921 cgctaatttc ccataacgat taaaccgaga atcatctaag aaagtcgtat ctgctgacca
    10981 attagccgaa taaggtgggt tcattactac tgcatcaaat gtgtaaggtt catctgttgg
    11041 ccagtcctta ttcaacgtat ccccattgcg gagattcatt tcttcggcgt cgacaccatg
    11101 taaaatcaag ttcatttttg ctagattgta tgtcgttgta tttagttctt gcccatgata
    11161 tttaacgtta tctgggtgtg ttaagtagtt acgtacgttt aacatcaatg aacctgaacc
    11221 catcgttgga tcaaaaacac taaagaatcg tcgttccttt tgatcgagcg ttacgatttg
    11281 cgccatcata tcagaaacca tatgaggtgt gtaaaattct ccggctttct tccctgcttc
    11341 tgaagcaaat tgactgatca gaaactcata cgcatccccg atcacgtccc catcgtgttc
    11401 taaaacatct acatcattca atttcttgat gacttcggta atcgtcacat ttcgttgttg
    11461 ctcatctgtt cctagttttt ttgattggag atccacatca tcaaataacc cattaaattg
    11521 attgtaggta gatgccaatt gcacaaaggc tttgtttaga tcgttcaatt ggaacgttgc
    11581 ttgttttgct tgatccgcta agacgttgaa aagatactct ggtgcaattg catatcccaa
    11641 aatatcaacg attgtggcaa tcagatcttc ttttgattct tcatctgaaa gtaattcacg
    11701 atacaacatt gtttgtttgc ttactgtgtc atattcctct aaggattcat ctgctaaaag
    11761 aaccacttgt tccaacaatc gatcggataa atatttatag aaaatcagtc ccaataaata
    11821 atttttatat tctgacgcat ccattttact acggagatta tctgccgcac taaataattt
    11881 ttgatttaat tctgcaccca aaaagataca ctcctatctc ttatttaaag ttgctatata
    11941 ccctcttatt ttagcatacc cccttttata aaaagcaaaa aaaaaagaaa gaaacttatt
    12001 cttgctaata aactacgcga aaggagtcta acgttgtttg atataactta gactgatcgt
    12061 taacttattt tttatatttc tacatagtta tctttagaac catttcagat acatatgaaa
    12121 caattacata tgattttttc ataaaaaaac agtactgtct agtagtataa aatatacaaa
    12181 tttcttaaaa attttttcac taaaatcact acctgaaaca gtctaaaaca cttatatatc
    12241 aacgtttaat gaaactatct ccgtttcatt tcagatacaa ataaatatag tcgggctcca
    12301 aatccgaaaa aaaatacccc cagtagctta tcctaagcta ctggagatgt ataagtacgg
    12361 tcatataaca acggggtagg gtaaataaat gaaaaagatg gaagaatcat tattcaataa
    12421 cttttttaaa tacttcgttg taatcagtta cctgtccgtt cttaaatacc acagaataat
    12481 ctttttaccc attttcaagt aaccgaagtg gaatgtcttt accaacttgc tctgtcactg
    12541 cattactgat acctgttaga ttagctaata aatctgataa agattgagca tctgtagaaa
    12601 tctctgtatc agtatttatc gtgattcctt tatcttcatt tacaagcttc caagtaggtg
    12661 gtatagctgt gtatgtttcg atgaatgaat caattctttc atctatcgtc ttcccagtat
    12721 ctacttctgt ctcattactc ttagatgaat cgattctttc atccatcgtc tgctccgtaa
    12781 ctgcttctgt ctcattattc tcaccggaaa ctctcttcac acccaaactt atatattgtc
    12841 catctttagt gtgcatagtt aaaattccca cgcttttact aataaattca ctataaccag
    12901 ttctttcaat agagacttca atgtatttca cactatcata ctcaaaagaa aaatagacat
    12961 gtaaaagcat tttgttgttt acatctacat aataatcctt tgactctact tgagaccttc
    13021 ctatacggaa tacagcttta ctgtctgata tctctatacc agcatcctta ttattaatct
    13081 gccaatctcc aagtagctct tctggcaagc tagtagtttc ttgtgaacac tttaccactt
    13141 cactggtaga ggtcgaaaca ttcacctccg cactgttaga agaattttcc aaggaatcag
    13201 gagccttagc acaaccaaca agtctttata tattgtgcag ccgatatttt cttcattcag
    13261 tacacccttg aaatatgcag tgttagataa agcagttcgt cctgatttgc tgaggcaccc
    13321 atctcttctt gaacgtactc cttgattttc aaggccgccc gatactcttt cggaaaacgc
    13381 tgcttcactg aagccagcaa aaaatcatcc ttctccggca actcttcctt cttaatcatt
    13441 cgggcaataa aaaactgtaa atgcgtcaca aatcttaagt aaagcatcga atctttatca
    13501 accttttttt cgggaaacag atatcctagc atagccaaga tatcctttag tgctcttacc
    13561 gagagcatgg tttcttccat atttgcttgt tcactttggg catttacaat gtgaaacgca
    13621 atgtttccag cctcctcttc tggcaactga acattcattt ttttgttgat caattggata
    13681 gcatagcagc cgatctcaaa ttcttttggg taatactttt gaatttcaat cagcaggcga
    13741 ttttgaaaaa caatgccttt ttgactgcgt tcaatggcat gcttcaagtg atcaaccaag
    13801 gtaaaaaata gacttttatt cagcttaaag tcaaacttgg cgttggcata atcgataatt
    13861 cgcttgacgc aaataattag atcatccgga gcagcctcaa acagtttaat gtaatccttt
    13921 tttagagcat cagaaagtga atctaattta aatacttttt ggattttgtc agccggaacc
    13981 aaatcgcctt tctttagttg aaatccaatc cctttcccca tcaaaattac ttctttgcct
    14041 tgcttatcta agcaaagaat taaactgttg tttaacgttt taattactct cattagattc
    14101 accgttctct ctaagctaag ccatttcttg cgattacttt ttgataccaa aagaagcttt
    14161 ctttttttat tcgttttaat tctttaagat tctcatcggt tcggtcaaca tatataaaac
    14221 cataacgctt attgaagcct tgatgggtac taacaacatc aatcgctgac caaggtaaat
    14281 aaccaaaaac atctacacca tctgttaaag ctgcttgaat agccagaata tgtttggcta
    14341 gataatcaat tcgatcctgg tcattaattc gtctatcttc ctcaactatg tcgggtgccc
    14401 catagccatt ttcagtaatt aaaataggta aatcatagcg atcgtacaat ttcctcaatg
    14461 ttaatcgtag tccagtaggg tcaactaccc agccatatgg cgttttatct acataaggat
    14521 tttcagctgc ccggtagaca ccttcttctc ccaacatgat ctgttgatcc cccgctctag
    14581 cggaaacatc tgatccgtca tttttgctgg cagcgatggt ggcagttgaa taataattaa
    14641 tcgcaataaa atcgggttta ccggaggata aaattgcctg atcctctgcc tcaattttag
    14701 gatataatcc acgatcattc aaatagtttt caaacaaata attgtatttt ccccgaactg
    14761 ctacatctaa gaaagaccag cctctcaatg tttcccaatt atgggctgca attgcatctt
    14821 ctggactgga ggtagcttga tacatagccg ttaaattgat tgctggaccg atttttcctt
    14881 taagattcaa ctgatgatac aagtgaatta ctctagcttg agctaataaa acatggtgat
    14941 taatttgaaa gagttctttt tttgaaggca gctctccact tggtggcaaa ccaatcgctc
    15001 caggatgaag gatcatcgtg ttttgctcat taattgtcag ccaatagttc accttgtcac
    15061 catactcagt aagcaatacc tgtacatagt ccacaaaata atcgatagtt gctcgattta
    15121 accaaccgcc ctgctcctct agaaaataag gtaaatcgaa atggtacatg gtgacaatcg
    15181 gttcgattcc ctgattcagg cattcatcaa ttacttgatg ataaaaagaa attccctttt
    15241 gattggtttt attattttca atatccataa ttcgcgtcca agcgatggaa aagcgaaaag
    15301 cttttaagcc taattcttta aacagctgta tatcttcttt atagtgatgg taaaaatccg
    15361 aggcaacttc aaaattagct accccttctg tatgctgata cttgtcaata atagacggtt
    15421 gtttcccatc ctcctgatag gcaccctctg cctggtaggc actaatggag gcaccccata
    15481 aaaacttctc tggaaatgct gaaagctgtt tgtattgcac aaaaatcctc tccttaacta
    15541 atttgcatca atggttcgtg ttcctttaag cttccgcttg cttcactaat tcctgcgtaa
    15601 ttttcactgt tggtcactac cacaatcact gtatcatcgt agccagcgtt tctaatggca
    15661 gcgaaatcgg cgttgattaa taattgtcct gctgacacct gatctcctgc ttttacaaat
    15721 acttcaaagg gttccccttt taaattcacg gtgtcgatcc ccacatggat caataattca
    15781 ataccttctt tagaacgtag accaatcgca tgtttagatt ctaaaatagt aatgacttct
    15841 ccctcaacag gtgaataaat gctgttttct tctggatgaa tggctacgcc ctttcccaat
    15901 agtcccttag aaaagacagc atcgttcact tcagaaatag gacacacggt tccttttacc
    15961 ggattttcaa caatgactgc ttcggctatt gccgtttgct ctgattcatc agaagtatcg
    16021 tcaaatccaa ttagataagc aatgataaag gttgcaacaa aggcgattac acacgtaata
    16081 attgcgtgaa caatattcat tggattttct ccaataaagg ctggtaaggc tgctaagcca
    16141 ggggaaacaa aggcatatct gactaaaccg ctcaagccag catacaaacc agcaattgca
    16201 ccaccaatca tggaagcaac taatggtttt ttcagcttca aggttactcc atagagtgaa
    16261 ggctcagtaa ttcccattaa agcagtgatc ccagtggaca cagctagctg cttcaactct
    16321 ttgtttttag ttttcgcggc tacagcaaag gtagcacctg attgagcaat attagaagcc
    16381 agcattcctg gcccattgat catttcagaa cccgtactgc tcaattgcat agtagcgatt
    16441 ggcgtcattg cccaagctgt acccgtaacc accaaaaatg gctgcaaacc acccatcaaa
    16501 aatggaatta accaagctgc tttgccgtta atccattcag cccccattgc tataagatcg
    16561 ttcagcaacg ttccaaacgg tccaatgaca accaacgcta aaggccccat aattaaaaat
    16621 accagcatcg gcttagtaaa gaatttaata aacgaaggcg aaattcgatc cgcaaagggc
    16681 tctacatagg acatgatcca aacagtaaag ataatcggta aaactgatcc cgcataatct
    16741 gcagctaata ccggaattcc tagaaaagaa acggcatcgc cagtagaaaa aagctgtgaa
    16801 atatttggat gaagcaaaat gccagcaaca gtaatcgcca aaattgggtt gacatcaaac
    16861 tttatggctg ccccataggc taatagtact ggcataaaat aaaaggctgc atcagcaata
    16921 gtattcaaaa tgatgtaagt ttgactactg tctgatagta caccagtcaa agtcaagacg
    16981 gccaaaatgg cttttaccat cccggcacct gttatagctg gaatcatcgg tgtaaaggtg
    17041 gtagaaatga cactaatcaa tcttgaaata atactttctt gattgtctga agaggggccc
    17101 tttttaattg aagttcccac ttccttttga atttccttga aggctgtttg gacttcattg
    17161 ccaatcacca cttggtattg tccaccgcta tccaccacac taattacacc aagtgttttt
    17221 tctaaggctt tcgtatcggc tttcctttta tctttgagtt caaaacgcag tctggtggca
    17281 caatgggtta agctgaccac attttctttt cccccaacct gttgcaatac atttcttcct
    17341 acttctctgt aatccattat tctctctcct taaagcagta aaaaagacca agtcaaacca
    17401 cgccccaatc aacattgaag tgttagtttg tcttggtctt gcctgcataa tcagtaacat
    17461 gccaataacg ttattttgct gtaatccaat aatagcacat tatgatagcg caatcaacag
    17521 ggaatttaat tttttatctt ttcgtttgac cgtatagggt aatataacag ttagatcaca
    17581 tcaactaggg tatatctgcg tatacttact gctaaaataa gtatatctaa tattaactat
    17641 accagtaaaa ccattcctac ataaccagcg ggaaattccc aaaatcgtga aagcatcaaa
    17701 aatgcatata acggatcaca caacttcaac aattctgctc ttgggaaaaa ttgatgaatt
    17761 tggtgagtaa aggatggaat aaacagcatt ccaacaatat aggcaacaat agtgatactt
    17821 gtttttttgg taaaaatcag taagcaatag ccaatttcaa tcattccgag atataaaggt
    17881 atcaatagaa tgatttgggc tagtattctt aaaaattaac tagcaaatgt ttaaactgat
    17941 ttatttctaa tctacatgta gtaagtattt caattccacc aattatcaat aaaaatacca
    18001 aacatatcca taataatttt ttctttttca tttcttttat attgttataa cagtgaataa
    18061 cataaaaatt cccgacaatc attgtaatat ttttttgtca cctgtattag tatttaattt
    18121 ataggaaact ataaaaactc cctagtgaat ttacaccttt gggagttcct gtttggacgg
    18181 tatgcataat acatttaaat caatttaaca ttaacacctc agcatatggt atctccacat
    18241 atatacggtc tttctttgga tagtactcaa tatttatgtt gatagcactt ccattgtcta
    18301 atatagcttt agcccggtat gtgttatctc cattttcatg agtaatcgtc acatcatcac
    18361 atgatcgatt taagttgtac tgttcttgta aaatctgtgt taccaatggt tttgatgact
    18421 tctctaatcc agatgtacct gtcatttttg aaaaaataat acctataata gccaagacaa
    18481 caatacctat aatgacaaac ttcttgttca tatttacttt attcaacttt ttatctccta
    18541 tctccaaaat aatttgttct tatccctctt atcagtacat tgacatccct aaaagtacac
    18601 caaagattgc tccagctacc cctgctatca tcaaaatcgt acccattttc tcatcttttt
    18661 ctcgatataa gtagcccata ataacagcaa cagcaccaaa tacaacagga ataaatgcta
    18721 acgatactac catagaaatc caaccaataa tcaaatacag cttccctgtc tcttgtccat
    18781 gtacgatttg ttccttcatt tcattaattg tctgctcagt acttttcttt actgtgtctg
    18841 ttccggttgt ctcatctgtt tcttgtagtg ctccacaaaa ctggcaatac ttactttcat
    18901 ctgttatttc tttgccacat tttctacaat acatgtcttc tcctcctaaa atcattcaat
    18961 tgcttattag ttaactgttt actttattaa atacatcaga aaacagctca ttcattggta
    19021 cacttaatat ctcagaaagc atcttcattt gatagggttt aggaatcgtt ctccctgttt
    19081 gccacgaact tacacattgc tgtgtaacat tcattttttt tgctaactcc gtttgagaaa
    19141 gtcctttttc ttttctgtat cggctgagat tacttacttt tttcatgatt ctcccctcct
    19201 gttttattat atacaacaat atgatgtatt tcaactcata aaatataaaa aacttcaaat
    19261 accattacaa atacaacaac ttgttgtact atactgagat gggggtgtat taatgaaaca
    19321 gaaacaatat gcaaaggtca ttagagaaaa gcgaaaagct ctcgggtgga cacaaaaaga
    19381 attagctgaa aaaatcttct ctacacaaca ggcagtagca agatgggaaa attctgtaac
    19441 agagcctaac ctcgatagct taacagctct ttctagagcc ctaggaaccc ctgtaagcca
    19501 ttttctagac aatgttgtcg tagattatga ggaagagttt ttggctctct atcgttctct
    19561 tagcactgaa gatgcagttc gaacaattga ctatatgaag ctattaaaaa ggcaagaaaa
    19621 tgaacgcaat cagctattga aagattgaaa aataaataac ccaccgaact ttaacggtgg
    19681 gtgtattgag atgaaataag attaagagct atggataacc tgtctagttt aatacaattg
    19741 ggtcttttct cttccttact ctccgtccat catctcttgt tacacttgta aattgataca
    19801 gaggaactat cccagctcct gataatatca attcttttat tgtttcatct atccctgccc
    19861 ctttcgctaa atatattaaa aaactactac ttgctttcac attgtatagt ttcttaatat
    19921 ccttagaagc tcgttcaaga gtgaatcctt gtttaacaaa gtagcctctg ttttttatta
    19981 aagcattttt cacgctgtta tccccaacat tacgtaggcg ttcacaaaca gtgttaataa
    20041 gttctttctc tgtcattaga tatgtccctt gccacgataa gccctgtaaa acttcatgtg
    20101 cttcatctaa aatcaattgt ttttccttgc tgagttctga tcgggaattt tgaggaaata
    20161 ctcttttagc ctctttttcg ttaaaacata tcttaaccca ttcataagaa aaatattgat
    20221 atgtaaaatg aattttttcc agaagtggaa ggatatcttc attcaatttt tgaatcacag
    20281 aagttgcatt aaaaacgtct aatcgataga cattgcttct cttatgatta aagtttccta
    20341 acgctttctc ttcctttaac ctatttaggt attcgagcat cttcaaagga acatcttcat
    20401 catctaattt ttttatcaat cctataaaag tcataagtga tagtaactta ttacatttat
    20461 ctatgtcata agttttacca tagtaagaac tcattttttt agctaattgt tcccccgaca
    20521 aaatacaagt cacactttcc ttactatcat caaaaaaatt aacatcagaa agcaagacat
    20581 catagacacg tcgcatttcc tctagataat tccttattgt cctgaaaagt ttgtctttca
    20641 ttgattcaag agtgtcaaaa agtacttttg attcctcttt caattttcga aaatgtgtag
    20701 gatacaagtc taggttctct aatagccaat taaatgtttc tacccgactg tctttcttta
    20761 aaattagcat cacaactcca ataaaatttt cactgatatc acaattactg gaatgacaat
    20821 aaaaaatgga atgcccagtt ttactggtaa aaatactagc agagggatta tgatcgttgt
    20881 gaaacagaca tcttaagttt ttacttgact taagtaattt attcatatca atggttaaca
    20941 atctttcata aatctcatta aaagacatgt cggaggaatc aaacaggcag tctttaaacc
    21001 actgtttcat ttcttctttg tcgtcatttt ttatcaactt aacaaaatcg gtttcattga
    21061 ctagttcata ctctctccct gaatcacttt gacaattttt agaacatgtc atggtaaaat
    21121 caatcggaag ctcagtaata tctaacatgt tgtcttcatt aattattatc actgcatctg
    21181 acttatatcc tccaaaaaac attctattcg aagcatttac attgtcatca cacccgggga
    21241 tgttcttttt caagtattcg taaacagcaa ccattttacc atagtcagta attgcttctt
    21301 ttaatagaaa gacaatcttg tatttttcta gattttcaga gtaccgaata cttttttgta
    21361 tcgcaaacgc agaattatga actaaattga tagataaaac cttttctatg cttaagtatc
    21421 tactatcatc tttactcaaa tctactacct tccccttctc atctttaaca gtgttatcga
    21481 catccagaat aattactctg gtttccacaa aactctcctt gtcattgcct ttattctttg
    21541 tcttgcttgg aataaatgat tgtgatgaat ttagtatctc tacaaactca tctcttgtaa
    21601 cttctctata gttatctaaa cacctatctc ttacatcagt tttttctttt tttgtaactg
    21661 gcttctctaa taatttttct tgatctacat aaattggaat attaatcatt gatgtttcca
    21721 tttgtgtttc ctccttataa aattctgtta aagactaaaa catacagata ataaaaacaa
    21781 actctttatt actatatgtt ttagtcttat ttgtttactc aacctttata ccgttccatg
    21841 aaactatcaa tatcagattt tttaaatcta atgaccgtat caatttttat aacaggtaac
    21901 ccgtattgag aaacatattt tctaatcgtt cctactgata cacctagata ttcacttgcc
    21961 tgctgatagt ccatatactc tagagtgact ccagaaataa tgtcttctag cataggcttt
    22021 agcactgtaa ttatctcttt cacttgttgt tcaacttgta tagaaacaat gctatcaatg
    22081 atttgtagag acatatttcc ccctcctttc gtgattgctt gaacaaattt tctaagaatt
    22141 atcttcgtat tttttgctca aaataaaagt attttgttat taaccgatta atcaaacaaa
    22201 aaatgattac cagttcatcg acatacacat aatacctcga taaaaaaggg gtgtcaacag
    22261 ctttgtaaaa tttatttaca acaccccttt tttgaggtat actaatttta ggaggtgtgg
    22321 tcaatatgat aaaacgtcca ccaataaatt atttagaacg aaaaaagata ctcggaacaa
    22381 aaataaaagc tataagaaag agtaaaaaat taacacaacc agcatttggt cttatgatta
    22441 acaacggtca attaattgac aaaaaaacta tctacgaatg ggagaaggga acctatttac
    22501 caataccaga gcgattatcc cgaattgctg atttgggaaa tatgagtata gaggagttag
    22561 tttgtggcaa tgtagaagaa tatattttgg gaattattct ttatagagat agcattgtac
    22621 tagatggtat aacatttcca gataaaaacc tgttccagca tcttcgtcaa cagttcccac
    22681 cagtacactc taatttagat acatggctgg atagatattc caaattagaa ccagagatgc
    22741 aagaatttat tgctaacaag acttgtaata aagttaaaaa tgaaaaaatt agtcttttca
    22801 atatcttaaa aatagaagaa ttattcatca atgctattgt agaagaattt gacaataaca
    22861 ttcttttcct cactagcagt atagaagaac tactagaaag aatggtagat gaatggctac
    22921 ctattcaatt gaaagacatg tcttatcccg aagaagctgt gagagaaatt actgataata
    22981 taaacaagct agaacaaacg atttcatcaa ttgggaaaaa atacactaag aaaaaaatga
    23041 agggaggtga taccatctag tggtaagtac tccttgtaaa ctaagaggag atgatttaat
    23101 ggcaaaaata tcaaagtaca aaaagaaaaa cggtcaagtg gcgtggatgt ataaaggaca
    23161 cattgccacc gatccacgaa ctggtgaaaa aataaatact acgagaagag gttttagtag
    23221 caaacaagaa gctcaaaaag attatgacga gtacagacat caaatattat acggtgttaa
    23281 aaagaaagca cctgatatga catttgaaga cctgtataat gaatggataa ctcatcagcg
    23341 taccagtgtt aaagcttcaa caatcgcaat ctcagtaaga tacgcaaata atcaaatact
    23401 tccagcattt ggtaagctta aactatcgaa tatctctgtc ccatattgtc aaaaagtggt
    23461 agatgaatgg cacagtaaat atgaaagtta cgattatatg agaaaacaga ctgcacaaat
    23521 tttacgctac ggggttgcaa tgcaatatat tgacaataat cctatggaaa aaacacttct
    23581 tccacgaaaa aaagagtatg agaaaaatcg taaattttac tcaaaagagg agcttaataa
    23641 tttactggat gcttttaaag attttggtaa catgaaacaa tacgccttct tcagattatt
    23701 ggcatacaca ggtatgagaa agagtgaagt actcgcttta cagtggaaag acatagacac
    23761 gtttaataaa gaactacatg tcaataaaac tcttgctgta gatgaatttg gtaaagttat
    23821 aattcaatct ccgaaaacaa gagcttctag acgagttatc tctttagata ctgagactct
    23881 ttctatcctc aacaactgga aattacagca aaaagaagag tatttaaaac ttggatacaa
    23941 tactagctct aaagagcaac atgtttttac aacagttaaa aacactctat acattccaaa
    24001 cactgttaat gattggttaa gatatatttt gaaaaaatat aacttgcctc gtattacacc
    24061 tcatgggttt agacatacac atgctagttt attattagaa gcaggagaat ctgtcaaggt
    24121 agttcaacaa cgattagggc atgaaaatag taaagtgaca ttagacatct atgcacatat
    24181 tacaaataat gcacctaaga aaacaggtca agactttgct gatatgatgg ctcatcagta
    24241 aaagagctgg tcaaaatctg gtcatttcta gtatacaggc aaaacaaaaa agctagaacc
    24301 cttatatatc aagggttcta gctcctataa caggtttacc caacactgcc ttccatctca
    24361 taactgatca atcgattgag ttcgactgca tattccattg gaagttcttt ggtgaatggt
    24421 tcaacaaagc ccatgacgat catttcagtt gcttcagatt cggtcaggcc tcggctcatc
    24481 agatagtaga gttgttcttc tgagattttt gagactttgg cttcgtgttc taaagagact
    24541 tggctgttgt ggatctcatt gaatgggatc gtatcggatt tcgaccattc gtccatgatg
    24601 atcgtgtcac attcgatatg agagatcgaa cccgcactgt ttttggcaaa ggttacttgt
    24661 cctcgatagt ttacttctcc gccgtctttt gaaattgatt tagagacgat agagctagat
    24721 gtatttggtg cattgtggat catttttgca cccgtatctt ggatttggtt tgctcctgca
    24781 aaggcgatgg agagcatggt gcctcgtgca ccttttccat ctaagtaaac acttgggtat
    24841 ttcatcgttg tttttgcacc taggtttccg tcgatccatt caacggttgc gccttcgtat
    24901 gcttttgctc gtttggttac taagttgtac acattgtctg accagttttg gatcgttgtg
    24961 taacggcaat acgcatcttt tcttgtaaag atttctacga tggcggcatg caggctgtta
    25021 cttgaataag taggtgccgt acaaccttcg acatagtgga tgcttgctcc ttcgtcaaca
    25081 atgatcagcg tacgttcgaa ttgccccgtg ttttccgcat tgatccggaa gtacgtttgt
    25141 aatggaacat cgactttgac gccttttggc acgtaaataa aggtcccacc tgaccatacg
    25201 gctgaattca gcgctgctaa tttgttgtcg gttggcggta caagttttga aaagtactct
    25261 ttgaataatt cagggtattc tttcaaagca gagtcagtgt cagtaaagac gattcctaac
    25321 ttttcgaatt catctttcat attatggtac acgacttctg attcatattg tgcggaagca
    25381 ccggctagat aagctcgttc agcttctggt ataccaatct tttcaaatgt atctttgatt
    25441 ttttctggta cgtcttccca gtcacgagcg ggtttgtcac ttggtttttg gtaatatttg
    25501 atggcatcga aatcgatgtc tgaaaggtcg gggccccatg tttgcattgg cattttgtta
    25561 aatgtttcta gagatttcag acggaattca agcatccatt ctggttcgcc tttgattctt
    25621 gacatctcac ggatcacttc ttctgtcaag ccttctcctg tactgaaaac cggttcgaca
    25681 tcatcgtgaa acccgaattt atattcttct aattctggta cgcccatgtt ctcactcctc
    25741 ttccttgtgg taatgatgga aagctgcggt accatcgttt tccattgctt ttcctagtgc
    25801 tttccaggca agtgtcgcac atttgatccg agcgggaaat ttagctacac caccaagcat
    25861 agcagcatcg cctaattggt cgggatcttt gacttctttt ccttggacaa gctgtagaaa
    25921 gtctacagtt aaatgttctg cttcttctat tgattttccg atgacggcgt ctgtcatcat
    25981 cgaggcactt gccgtactga tggagcagcc actgccgtca aaggcgatat ttttgatgat
    26041 tccgtctttt acatcgactt gcaattcgat cacatctcca catgtggggt tgttcaattc
    26101 gatcttgctt gtcgagtcag tcaagcttcc acgatgatgc ggatgggaag aatgatccaa
    26161 gatcacttga cggtataaat tgtctaattt agatagtccc atgttggaaa aactcctttg
    26221 ttgccatgat tgcttcaact aaacggtcgg cgtcttgcgt tgtgttgtag aaataaaagc
    26281 tcgcgcgagc agtcgctgat acttcaaggt acttcaatag cggttgcgca caatgatgac
    26341 ctgctctgac ggctacccct tccatgtcta aagctgtcgc gacatcatgg ggatgtaacc
    26401 catccaaatt gaatgcaacg actcctgtcc gaaaattcgg gtcttgtgga ccataaacag
    26461 tcaatccatc aattgcttgt aattttggca acacataagc ggctagttct tcttcatatc
    26521 gatggatatt gtccataccg agtttttgca aataatcgat tgcatggcct aatgcgatag
    26581 cgccggcgat atttggtgtc cctgcttcga atttccaagg tagttctttc catgtactgt
    26641 cgtataattc gacaaaatcg atcatttcgc caccaaattc aacaggttcc atttcttcta
    26701 accattttcg tttaccatat aaaacaccaa tacctgttgg tccgcacatt ttgtgtccgc
    26761 tgaaagcaaa gaaatcacag tctagtgctt ggacatcaat ggccatgtgc ggggcagatt
    26821 gagcaccatc tactacgatg accccgccgt tttcatggac aagttcagct aattcttgaa
    26881 caggattgaa gacacctaag acattggata catgagcaat ggagacgatt ttggttttgt
    26941 ccgtaatctt tttcgctgca tcctccatat ccaggaagcc ttcttctgtt agttcgatat
    27001 atttcaagac agcacctgtt cgttttgcca actgctgcca cgggataaca ttagaatgat
    27061 gttccatgta agagagcact atctcgtctc ctgctttgat atagcggtcg ccgaaggttc
    27121 tggctaccca attcaagctt gtcgtcgtac cacgagtaaa cagcacttct gctgtttctt
    27181 ttgcatggat gaacgcccgt actttttctc ttgcttgttc ataatcatgg gtcgcgcgtt
    27241 ctgctaacgt atggacaccg cgatgaacat tggcgttatc gtgttcgtaa tagtacgcta
    27301 atgcctggag tacttgtttt ggcttttggg tcgtcgctgc attatccaga taaactaaag
    27361 gttcatcatt gacgtcttga aataagatag gaaaatcatt gcgtagcttt ttagcatcga
    27421 tcatccattc aacttccctt cgattacttc gactaattct ttccgaactt gttctacagg
    27481 gattgctgtt aagacagaac cgaggaatcc tcgaatcact aagcgctctg cttcttcttt
    27541 atgcaatcct ctactcatca gatagtacat ttcttctgga tcaactcgtc ctacactggc
    27601 agcatgtcca gcagttactt cgttttcatc gatcaaaaga ataggattcg catcaccacg
    27661 tgctttatca gaaagcatca agacacgact ttcttgttgg gcatctgcac cttttgcccc
    27721 tttcaagatg tggccgatac cattaaaggt caatgttcct ttttctcgga tcactccgtg
    27781 ttgcaaaata tgcccgatcg tatgaggggc tttattggtt actcgtgtat cgattccttg
    27841 gatttgtctg cccgagctga ttgccacgat tttgacttct gcgtgtgcac cttctcctgc
    27901 taaatctgag tcaaagtcag cgatcacatg accgtcattc atgacaccaa ttgcccagtc
    27961 gacagaagca tctcgtaaaa tgtgcccacg gcgattcata taagaagtaa tattttctcc
    28021 taattgatct acggctgaat atttgatttt tgaaccagct ttagcgatga cttccacgat
    28081 gatattacct gatgattttg ctacttgttc tttggttgtc tggaaacgtt ccagataaga
    28141 aaattcacta tgttcatctg ccacgatcaa cacatgcttg aagaagtgct catcggatgc
    28201 gccatcttga atgaataagg actcgagtgg ttcatcgatc acgacatttt taggcacata
    28261 caggaaaata ccgctgttca taaaagctac atggagtgca gtcaattggt cttcgttcat
    28321 gtttactgct tttttcatat aatattcttg gactagttct ggatattcga tcattgcagt
    28381 gaacaagtcg gtaaaaatga ctcctttttc tgctaattcc acaggcagtt gttcaaaaat
    28441 cgtccatgaa ccttgttgga caagcacagg attgtctttc attgcatcaa atgccggaat
    28501 cgtgcctgtt tcactgatgg tttgtgtttc tttcacatca aataacgacc agcgatggaa
    28561 tttcacccga tcgatatgcg gcaatggcag ttcatctgct ttcgctagtg cgtcttgacg
    28621 gaaggttctc atccaatcag gttcgccttt acttaaagaa aaatcattta ccgcgtcaag
    28681 ataatcgagc cagttttttt ctttcattct tgctccccct tacgcttctt cttctttata
    28741 gtcgattcct aattctttac tgataccagc gtagccttct gcttccagac gtttagctag
    28801 gtcagcattc cctgtcatga ctactcggcc ttccatcatg atgtgaacga catcaggtgt
    28861 gatatagttc aataatcgtt gatagtgggt gatgatcaat gcaccgaaat tgtctccgcg
    28921 catttcattt actcctttag caacgacttt caaagcatcg atatccagac ctgaatcgat
    28981 ttcgtctaaa attgcaaatg taggttctaa catcaacaat tgcaggatct cattccgttt
    29041 cttttctccg ccagaaaaac cttcgttcag ataacgttca gccatttctt ctggcatatt
    29101 tagtaaagcc attttttcat ctaacttttt caaaaatgcc ataacagaaa ttttgtcgtt
    29161 ttcatcacgt ttcgcattga tcgctgcacg catgaattcc gcattcgtga tacctggtat
    29221 ctcacttgga tattgcatag ctaaaaataa gccaagtcgc gcacgttcat ctacttccaa
    29281 gtctaaaaca tttttcccgt caaacaaaat ttcgccttct gttacttcat agtttggatt
    29341 gcccatgata gcggcagata aggtagattt tcctgtgccg ttaggtccca tgatggcatg
    29401 gatttctccg gttttcattg ttaggtttac gcctttaagg atttcttttt cctcgatcga
    29461 cacatgtaga ttttttattt ctaagacaga catgcattat acccccaaat tctctttcgt
    29521 tctcactcat tttagactaa ttgagaatga agcgcaatga ccttccttac taaaatcaaa
    29581 tatcttttat ttattattaa acaattttga tttctaaatt agaataggta taaaaaagat
    29641 gagaaataag ccattacaga cttatttctc atcttttttg ttccatacct tattttgcat
    29701 cagcctttac tggtttaaca gagccgcccc atttgtctgt gatccattct tggacttctt
    29761 tgcggtgaag cacttcaatc agttttttga ttttgtcact gtcttcatca cctttacgag
    29821 tcgcaataat attcacgtaa ggagagttgt ccttttcaag caatacggca tcttctaatg
    29881 gattcaaccc tgcgccgtaa gcgaagtttg cgttgattgc aactaggtca ccttcatcat
    29941 tttggtacgt agaagctaaa agtgatgggt cgatcgtatg gttgaacttc aagttcttag
    30001 ggttttcttc aatatcatcg aatgttgctg tttccagatc tactccatct ttgactttga
    30061 ctaagccagc atcttgtaga attgtgatga tgcgtcccca gtctgattca gaatttgaag
    30121 taatgattgt tgcaccatct tttaattcgg aaacatcttt gactttttta gagtacaagc
    30181 ccattggttc gatgtggaca gcaccggcat tgacaaagtc atatcctttt tctttgattt
    30241 ctttgttcaa ataaggaata tgctggaagt aattggcatc aatgtcgcct tcttccaaag
    30301 attggttcgg taatacatag tcatcgaatt tgacgatttc aagatcgatt ccttcatctt
    30361 tcaataatgg tttcacatgt tccaaaatct ctgcgtgcgg tgtcggtgaa gcgccgactt
    30421 tcaatgtcgt gtcgtctgct ttatttgctg aatctttgct gtcgctgttg ttaccgcatg
    30481 ctgctaatcc tacagtcagc aaaagcgctg ctgcaaatcc aaaaattttc tttttcatat
    30541 cctacatccc cctatatcct attatctttt gtctgttttc ttagttaaga aatcaccgat
    30601 tccttgaacg ataaatacga ttagtaaaat aattaaagtc gcaacgaatg tcactgttag
    30661 attaccacgc tggtagcctt cctgccaagc aagacctcca agtccacctg cgccgatcgc
    30721 gccggccatc gcagtaaatc cgatcatcga gatcgttgta actgttaatc cagaaatcaa
    30781 tgcgggaata ctttcaggta tcaagacctt ccagatgatt tcccagtaac ttgctcccat
    30841 tgcatctgct gcttccagta caccagggct gacttcacga aaagcaatct ctactagacg
    30901 ggcataaaat ggtgctgcgg ataaaacaag agctggaatg gctgcttgcg cacctagcat
    30961 agtgcctacg attgctttag taaatggaat gatcaaaacc atcaggatca taaatggcgt
    31021 tgaccggaaa atattactga tgatcgatac gattccataa atgattcgag catacggttt
    31081 gtttttgcgt ccaatagagt aaaggatcaa tcccaaaact aatccgatga tcgtcactaa
    31141 gaccattgag attaatgtca tatatagtgt atcaaggata gcctgattca tcgtttccgg
    31201 gttgatttgt ttaaaatcta gatatgtttc tgaaaagctt ttatccacga tggatcacct
    31261 caattcctac tccttgttct ttgatgaaca cttctgcttg atctaattct ttggtttcac
    31321 cagtcatcaa gacagtcaat gaaccaaagg aaccttcttt cacttgtttg atctttccat
    31381 acacaacgtt gacatcgatg ttatagcgtc gaatcgcttg ggaaatgact ggctcgttgg
    31441 catttctttc gctaaaggtc aaagttgcca aacggccgct tggattttct ttgatgagtt
    31501 cagctaatag ttctgtcgaa tcagcttctg gttcgatgtc ttgttgtaca aagcgtttgg
    31561 ttacttcttt tttcggttgt ctgaagacag tcaatacgtc tccttcttct acgactttcc
    31621 ccaactccat cacagcgact ttgttgcaga ttttgcggat cacattcatt tcatgtgtga
    31681 tcaagacgat ggtcaaattc aatttcttat tgatcgctaa caataagtct aatacttctt
    31741 ctgttgtttg cgggtccaat gcgctagtag cctcatcaca aagcaggatc tcaggatcat
    31801 ttgccaatgc tcgtgcgata ccgacgcgtt gtttctgtcc gccggaaagt tgtgaaggat
    31861 atgcttgtcc gcgtccttct aacccgacta aacgcaacag ttcttctgcc cgttctttac
    31921 gtttgctctt tggtacgcca gctaattcca gcggcagctg gatattttca agtacagttc
    31981 ttgaccataa caggttgaaa tgctggaaga tcataccgat tttctttcgg aatgtacgta
    32041 attctttgtt ttttaatttt gtaatatctt gatcgtttac gatcacttcg ccgctcgtgg
    32101 gaagctcaag tccattcagg agacggacta atgtactttt tcctgcgcca gaatagccga
    32161 cgattccata aatatcccct ttttcaacgt ttagtgaaac gtcattgacg gcttggatgt
    32221 ttccgttttt cccattgaat gtctttttga cattcgttag ttggattaat gacatgccat
    32281 tcacatcttt ctcttatgat ttttcaaaca aaaaagcctt cgccctcttg ccttaagcaa
    32341 aaggacgaaa gcttgaactt ccgtgttacc accttgattt accgttactt cgcaataaca
    32401 gcctcttcca gtacttggac ttagcttctt tccgtatact gtcgcgctgt aacgggcact
    32461 cccgtagtaa gtttgcttac gctcgcttac tctgctctaa gaccatcttc catctcattt
    32521 tcgttaccca ttttcagcaa acagggctct cttcaaacgt ccgtgacatg tactcttctt
    32581 ttcaaagctt tctcgtttca tataggattg cttatgatta tagcaactgg aagaatcagt
    32641 gtcaactttt ttcaagtgat tttctgattt tataattcgt caaattcttt tggatcaaca
    32701 tctttgaaac ttaagatcca cgcattcatt tcatctgaat cattcaagat attgatatct
    32761 tcaccgattt tatcattaac agaagaaact gtacccgtta gcgggctggc aaattcattg
    32821 accgcttttt ccgcttcaac ttcgatcaga ggttcatctt tttttagcga ttgtcctgct
    32881 tttggcagat tggcaaacgt gatgtctcca agttcttctt gcgcttcatt tgtcaaacca
    32941 acacagtatt cttttccatt aaataagatc caaaggttgt cctttttctt caaatgcttg
    33001 cctgccattt tatatcagtt ctccttcata aattttttca ttgaatccaa ttgcaacaag
    33061 ctggtcctct cgtaagatca gtggtcgctt gatcagcata ccatctgtcg ataagacttg
    33121 gctcgctttt gaagccgtaa actcgcctac ttgatctttc agtccaagtt cccgatattt
    33181 catcccgctt gtgttgaaca ggcgacggat tggtacttga ctcgtttcta gccaagtctc
    33241 aatcgttgag gccttcggta cttctttgat catatcaacg gtttgataac taacgccatg
    33301 ctggtctaac cacgctttgg cttttttgca tgttgaacat ttcgggtacc agtaaaaggt
    33361 gtacaatttt cttacctcct tgaagatctt acaggtgctt cattttggta tctcatcgat
    33421 aatatcaacc cgataccgat catgttccct aaaatcgagg atcctccctg actaatgaaa
    33481 ggcaatggga tccctgttaa aggaagtaat ccgatatttg ctccaatatt ctcaaagaca
    33541 tggaacagca gcatcatgat caaaccgctt gcgatatagg cgtaaaattc attattcgta
    33601 tcgaaacaca cccgaatcat acgatagatt aaaataaaat acaagaaaat gacgaatgca
    33661 ccaccgataa agccgaagtt ttcaccgata acggtaaaaa tcatatcgga ctcacgaaca
    33721 ggcacataga tattgcttac gttgaatcct ttaccaaaca agccaccggt accaattgcc
    33781 aaaagtcctt gggccggctg ataactcatt cctgacgtat catggaaagg gtccagccat
    33841 gaatcaatac gtgcaaactg ataagatttg aatccaactt tataaagcaa gtctcttccg
    33901 ccttcagtcg taaccaagaa gatcgttcct gctccaacga ctactgccaa gattactaca
    33961 ggtgcgatga tgcgccaaga gattccagac atcaggaaaa tcccgccaaa gatcgcaaga
    34021 aagacaagca tcgttccaaa gtcatcttgg gccatgatca atgctaaaac cggtattgtc
    34081 acgatcaaga gcttcccaat cagccaaaag tccgatttca tcgtccgttc ttttacttgc
    34141 gtattatgtt tagttacgat cagtgccatc atcaagatgt acgcaatctt catcaactct
    34201 gctggctgga aagtaaacgc accgaatcga aaccagtttt tagaccctgt tgaagcagct
    34261 aaattctgat catagaattt gagcaagagt cccattacca ctaaccccaa accataaagg
    34321 taaggagtca gtttccagat ccatttggaa ttcaaatgca tgatgacaac gattgctgcc
    34381 gctccaacta aataccagac aacttgcatc cctacgcctt tcaagactgc tggattgttc
    34441 ggatcatggc taagtgctac atataatgag agcagaccaa tcaaacataa cagaaagact
    34501 ggtaaaatca cgccataatc aatacgatta tcgttcgaag cttttaattt tttttccatg
    34561 tttcatccct tttcttaaaa accatactgc tgattattat agcggtccag ttagataatg
    34621 gaaagtcttt ccatagacat ttgggaaaaa ttaactttcc gtttgtgcag ttgcttgagc
    34681 aaatgcagta caaaagccta ctgcttatct ttagtgtatc atagaacttt ttttgataca
    34741 tgcaattatc taccagctga gattctcctc gctgtgagaa gtttctgtgc tttcattgta
    34801 tcgaaggatc tcttcggcaa tctctagcag ctgatcagaa accagattgg ttcgttggat
    34861 gaaataaaaa tatcgatgga aattcaacgg agtcattggc acattttctg gaacagagcg
    34921 cttagacaaa atcgagcgac cgattccttc cttcagcaac gcaacaatta catcgttact
    34981 tttcacgatc atcttcttat cttggatatt catctcttca aaataacgct gcgtgtaatg
    35041 aaaaacacca gagtttgctt ctcttacaag ccacaatttg ctttttgggt ccccagcgat
    35101 gactagctgg tcttccatca aagagtatct ttgtacgccg gaagtctcta atggcttttc
    35161 gataaaacca aaatcggatt catgtttcgc tacttgttct aatgattcat gagaattcgt
    35221 gatttcgatc tcaaaatgca ctttaggaaa ccttttgatc agatgtttca tcaactgagg
    35281 caaaagatag acagcaaagg tgtgggaagc aacgatccga catacttctt cttcattttg
    35341 ttcattgagc aatttttgct gagtttcttc ccattcttct aacaaaacca gtgtccgttc
    35401 atataataag tcggcttgct tagttggaag catctctttt cttccgttac gttgaaaaag
    35461 atcgatattc aattcccgct ccaactgttt gatttgattc gagactgctg gttgagataa
    35521 aaacagtact tctgctgctc tggaaaaatt tcttgtttca tatactgctc gaaaagtcgt
    35581 taataactta aacatggcac cacttccata actttttatg ataattacta taaaaactat
    35641 ttgatttaaa tatacaaaaa accatctata atagaaatgt acaacaaaac agttgctgat
    35701 ccaactagta acttagatga agacaatcag ctgtctgctt gctcgtaatc atacattcct
    35761 attcatttta caatgtattc gttcatcttg gtactaaaat ctattaaata aaaaggagaa
    35821 tattatatat gaagaacttt ttgaaaatta ttccgatccc gatctgcggg ttgatccttg
    35881 ggttagcttc ccttggaaat ctgtttaaag actatcatca tgttgcttta ggcaatctaa
    35941 ctggcggtat ctctatgatt cttatgatat tcatttttgt taaattgttt atcttattcg
    36001 aacacacgaa acaaacgttg catgatccaa tcatcgcctc cgtttcacca acttttacga
    36061 tgtcactcat ggttatttgt acttattttg tttcatatga aagtattgca ccaattgtca
    36121 aattcatttg gctgtgcgct gttatcttcc aagtgatcct tgtcttttat ttcaattatc
    36181 accatgttat caaagctgat ctttcaattg aagcaattta tccaagctgg tttatcattt
    36241 acgttggatt tggggtcatc actgtaactg ccgggaactt cttcccactt attggaaaaa
    36301 ttttcttttg gatttcattg atctgctatg cgttcttatt gccgatcatc atcaaacgtg
    36361 tctttttcgt aagaaatatg gctcatccga cacgtccgct gattacgatc gttgcagcac
    36421 ctggttctct ttgcctaaca ggttatctta aaaactttgc tgaacctaat ttttatcttg
    36481 tcgtagcatt gttcgcttta tctcaagtat tgtatttcgt tgtgctgtct atgcttccac
    36541 gtttgttcaa gcttcgtttt tatccaagct atgcagcctt tacattcccg cttgttatct
    36601 ctgcaacagc tatatttact actgttcatt acttcaacag tttaggtatc tatagtagcg
    36661 tattggattt cttaaaagtt gcggaatgtc tgtttgcaag tgtagttgtc ttctacgttc
    36721 ttgctcatta cgtacgtttc ttaattaaag aacatagcaa acatggaaaa tttgtttcta
    36781 aaaaaatcgc ttaataatcg attcaatagg ttgtgaaaaa gctgttctgc atcaagtaat
    36841 aagaacggcc aaacaaaaac agctgttcat gtttttgtac tggttgggct tattaccgca
    36901 gatgcaagct tttgaacacc gtttaccaag aggtcgtaaa agaagtggtc agcttcaaga
    36961 aataagaagg aatttacgaa aattgcttct caaatttttg tgaaatttcg gcttatttcc
    37021 gtagaagcta cttctggaac accgtttatt cgaaacaaga ggtcgtacaa gacttttgtc
    37081 acgacctctt gttcttttgt caaaatactg gtttagtgct actatgttcg agtatgcttt
    37141 tagtatttat tgacgaaagg aaccgatcaa atgaaatcat tgtcacatcc aataaaatct
    37201 gtaaaggatt cagcggtccg cgaaatctta cccggtttac tgctttcttt tgccatcgct
    37261 gccttcagca ggcttcttgc tatttatctg ccacagttag gtggagcgac tattgctatt
    37321 ttattgggta ttttattagg aaatactttt tttaaacagc ctgttctagc aatcggcaca
    37381 aaatttgctg aaagcaaact attagaattc tctgtttttt tactaggtgc aactgtcaca
    37441 gctcaaacga ttgctcaaat tggtgtaaaa ggaattgttt ttgtgttact gcaaatgtca
    37501 gtcactatca tcggtgctta cttgattggt aaaaaactgc aattcagcga tacttcttct
    37561 ttgatgatgg ctggaggaaa cgctgtttgt ggttcttctg ctattgcttc aatcgcccct
    37621 tccatccatg cgaaagagga agaaaaagga cagatcatca cactagtcaa tctactgggt
    37681 actgtgatga tgttgactct cccttttctt ggattacagt tgtttggtgg aaatctcatt
    37741 gaaaaaagtg cacttttggg tggtacactc caatctgttg gtcaagtagt cgctggggca
    37801 agcttacttg acaattctgt tgtcgaattc gccatgctat ttaagattct acgtattatg
    37861 atgctcgttg ttgttgtcta tctgtttgaa aaattcatgc acaagaaaca accagctctg
    37921 tttgaaacgc caactgatac gcagaaaaaa cgaaaattac cgcttccttg gtatgtatta
    37981 gggtttgtga tcttttgttt ggtcaacagc ttgttttctt tacctgtagt tattgaccaa
    38041 tcggcacatt ttatcagcag ctggtttgag atcactgctt tagcggcaat tggtctgcgt
    38101 ttagactttg caaaattcat gaaagaaggt acacgcttct tatcttatgg attagctgtc
    38161 gggcttctcc aaacagtgat ggctttacta tttatttggc tgatcaaagt ttagaatgaa
    38221 catgaaaaaa cagttgtgat tctcgcatgg aacaatgtcc gaatcacaac tgtttttaat
    38281 tttgcagata tccttctaac aacaacaatt ggcgtttgat ttcaacccca tctattgcgt
    38341 ttcccatata tcctgtcagt tttccatttt gcccgatcac tcgatgacaa ggaataacga
    38401 ttggcaatgg attggttcga tttgcctgcc caatcgctcg cacagctttc ggactatcaa
    38461 cagcaacggc tacttcttta tacgttcgga tttctccata aggaatggtc gacaaagctt
    38521 tccagacatt ttgctgaaaa ggcgtacctc tctttataga taaaggaaca tcaaattctt
    38581 ttctttttcc cgcaaaatat tcttcaaact gttcttgtgt gcgcgttaaa attcgctgat
    38641 tcgctgattc gctttctgaa attggctgaa aagatacagc agtcaatccg gattcatctg
    38701 catctaacca taaaaagccg ataggtgtgt gtattttcat tttgtttgct cctagtcaat
    38761 agactggaac ttcctccttt ttctctatcc aaaactactt gccaactaat aaaaattgtt
    38821 cttaaaagtt tgataaaccg aggaaacatg gtaaactaaa tacgttagtt tacaaaaaga
    38881 atacctgttc ttttacgtaa aatcaaattt taaaggtgtt atttaaggtt tattaaattt
    38941 tgaatgtaaa ccatatataa acatacgatt gaaaggatag aaacgatgaa agtatcaatt
    39001 gttgtcccat gttttaatga agaaaaaact gttcctcttt tttttgatga ggttgaaaaa
    39061 ttcaaaggtg cccatgagtt cgaatatttg tttattgacg acggatcaaa agacggtacg
    39121 ttagcagcaa tcaagaagct agcctcagct tatccttatg tccgctttgt ctcattttcc
    39181 agaaattttg ggaaagaagc ggctctgtac gctggattgc aagcttctac aggagattta
    39241 gtgaccgtga tggatgttga tctgcaagat ccgcccgaac tgcttcctga gatgatcaac
    39301 cggatcacta cttctgatat cgattgtgtc ggcacccggc gttcgactcg tgaaggcgag
    39361 cctgcgatac gcagtttctt tgcgaaaaaa ttctaccagt tgatcaataa aatcagcgat
    39421 acagaaatgg tcgatggcgc tcgtgatttc cgcttgatga ctcgtcaaat ggtggatgcc
    39481 gtcctagaat taacagaata caaccgattt tcaaaaggat tatttagctg ggtcggattt
    39541 aaaacagaat atatttctta tgaaaaccgc gaacgtgtgg ctggcgaaac atcttggtcc
    39601 ttctggaaat tattcaacta ctcgattgat ggtatcgtga acttctcgga tgcaccgtta
    39661 acgattgctt catttatcgg cgctttgtct tgtgtcggtt ctggtattgc aattttgttc
    39721 attattttgc gggcattgtt gtttggtgat cctacatcag gatggccttc catggtctcg
    39781 atcttcctgt tcattggtgg aatccaattg ttatgtcttg gaatcatcgg gaaatatatt
    39841 gggaaaatct tcttagaaac gaaacagcgt cctgtttata tcataagaga aacagaaaaa
    39901 gtgaagccaa atgaattgaa gcctcaccaa taatatacgt caaatgaagt aaacattttt
    39961 ttaaatgaaa aaggactgtg acaaaagtct tgtcacagtc cttttttccg aataaatggt
    40021 gttccagaag tagcttcttc ggaaataagc cgaaatttca caaaaatttg agaagcaatt
    40081 ttcgtaaatt ccttcttatt tcttgaagct gaccacttct atcacaacct ctttcatatc
    40141 ttattacttc ttcattgctg cttttagtgc tgctgctaat gggctttctt ctggttcttc
    40201 tgcttgtgca tattttttca gcaagcgtcg ttcttcatgt ttggtcattt tcttcttctg
    40261 ctctttttta tcaagcaatt tttctgtcgt tccatcgtat ttacaacgga aataagcacc
    40321 gtttttacct tcgatgattt ccattttccg gtgacattgc gggcatcgat gattagagac
    40381 ttttggatct tttcttctgc ggtagttgca atctgaattg gtgcagacat agattttgcc
    40441 gtcacgtgtg tttttctcgc gcagattggc cccacattct gggcatttct tttgggtaat
    40501 cgagaaatct tgatattctt gtttgcttga tttgatctca ttcaccaagc gttttgtttc
    40561 tttttcgatt ccattcaaga aggttcctgc ttgtttcttt cctgcagcaa tagcttcaag
    40621 gtctttttcc cagctttctg tcaaatctgg tgttactaaa gagggattta ccaatactaa
    40681 taattgctta cctttaggag atacttgcag tccttgaggg gtacgttcca tcagttcaga
    40741 tttgattagt ttctcgataa tctctgcacg cgtagctggg gttcccagac tgtatttttc
    40801 catttgacca agcaagcttg cttctgacaa tggtttaggc ggtgcagata gttctttttt
    40861 gatggcgaaa tcgggattga ttctcattcc tttttcccat ttgactgttt ccgtctcagt
    40921 ttcagtttcg cctttccatc ctgcttggac gacacgattt tgccgaaaaa tgaatcgttc
    40981 tttaccaaat tcaaccgtaa cttttgtttg gctgacttta tggggttgag caaacagtcc
    41041 taaaaaacga gagacaatca tttgatagat ttttcgctct tcattactta atttttcata
    41101 acgtgctctg ttttcagttg gcaaaagtgc atggtgatcg gtcactttac tgtcttggaa
    41161 tacttttgtc tgttggacga tggctccgtt tttcaaatac tgcttgacct caggagcaaa
    41221 gtcgctgact gcttgcaatc gttctttcat cgtacttttc atgtcatttg tcagatattt
    41281 gctgtctgta cgcggataag taacgatctt atgggtctca tacaaacttt gtacaagact
    41341 taatgttttc tttgcagaat agccatagcg ttgattggct tctctttgga tctctgttaa
    41401 atcataaggc aatggcgcag attccgtttt taatttttct tggacttctg tgacagttcc
    41461 tttgcttgta ctgatcaatt gaacaagttt ttgtgcttct tcttgtgttt ttaaagcaaa
    41521 ttgatttttt tgaagcattt tggcttgttc ttttccagct tgcaacgtga tcgtgaaata
    41581 cgtttgtgga cggaactgct cgattttctt ttcttggtct ctcactaatg ctagtgtcgg
    41641 tgtctgcaca cggcctgctg ataaattatc cgcgtacttc acagttaagg ctctggtcac
    41701 attcaatcct accagccagt ccgcttttgc ccgggcaaga gcagattcat acaatgcatc
    41761 atattctttc gcaggtctta gttgcttgaa tccagttttg attgctttat ctgtttgaga
    41821 agagatccat aaccgcttca ctggtttatt gaaatgcaca tactctaaga tccagcgagc
    41881 aacaagttcc ccttctcttc cggcatctgt cgcaatgact gcttctgaga catcttttcg
    41941 ctgagccagc tgcttgattg cttttaattg atgtcctgtt ttaggaagtg gtttgattcc
    42001 tagtttttta gggatcattg gcaacgtgtc catttgccag ctctgccatt ccttattcag
    42061 atcttccggc attttcaatc ctaataaatg accaagggcc caagtaacga tcaccttggg
    42121 tccttcataa taatttttgt gcttttgatt agcaccaagc accttgctta ggtctttcgc
    42181 aacgcttggt ttttccgcta gaattagttg tttcataaaa ttcctttctg ctttctactg
    42241 tgatgggctg atgatttttc aacaacgcaa gtcccttgta ttgctgtaaa tcatcgtcac
    42301 actcttcaca ccagcgctca tctccgtcat tccaaaaggc gctgagaaag ttactttttg
    42361 cattgatata ttcataattt tgatggattt cgttccattg tttttcgtac gcttgtcgtg
    42421 cttcttcgaa agactcgaat gtttcttctt cttcaatatc ttcttcccag tcttcaaaaa
    42481 accaccaagg ctcattgtcc ccgaacatgg taattacttg atacatactc gtgctccttc
    42541 tttcagcttt tctagactca tcttagtcac ttttcaagaa agttgcaaga attttctctt
    42601 ataaaagggt catttaatga acatcttaaa attgttacaa atgaatctga ttgtttaagg
    42661 caaagtagcg ataaagttat tgatcatcca aaaatagctg tcttctggat cgattgttgg
    42721 ttcttttgta aagtatccta aaaaacgtaa ggaaagataa ccgtggatca tactacgaag
    42781 ttccctgctt cgatgaactt tttcttcttt cgttaattga aaagcctcaa atatctgcaa
    42841 aatgatctga ttcgtttcat ggattccatc taataaaaca gcttcattgg tatgcggtac
    42901 actgatcagt aactcataca ctgcttgatt tgaaaaagca aaggatcgat aagtttctgc
    42961 atagatctgc agtgcttcct gaccactttt tcccacaaga gcccgtcgca gctggtcatt
    43021 aagttcctgc agaagtttcg ctgttagcgc tgttttgaca tcacgaatat ttttgaaatg
    43081 gttatacagc gagggagcct tgatatccaa gcgtttggct aaatgctgaa aggttatttg
    43141 ctgaacatcc atctcttctg ctagttctcg aaagcagtcg atgattttct cttgagacag
    43201 atttcttttt ttcatttcca tgtttcctct cataaaataa atccttcact ctttcagttt
    43261 accacgaaaa agaaagagaa aaaagagact ttcattgata aactacatat tgtagtttat
    43321 aataaagagc tgtagatgaa aattttcaga aagaaggagt ctaattggct gcaagaataa
    43381 aggaaaaatt atttaggaaa agagaaactt gggaacaaaa cttgtttgtc ttatggtttg
    43441 gaacatttat ggctggtatc ggatttagtt tagtcatgcc ttttatgtca ctttacatag
    43501 atacgttagg taattatagt acgtcacaat tgaacttttg gagcggattg actttttcat
    43561 ctactttctt ggtgactacg cttatctctc cttggtgggg aaaattagcc gaccaaaaag
    43621 gaagaaaatt gatgctgttg cgtgcttcat taggaatggc aatcgtcatt tctttgatgg
    43681 gttgcgtgac tagtgtgtat caattagtcg cattgcgtct gcttcaaggg attttttctg
    43741 gttatatcag taatgccact gcccttgtcg caactgggac gccaaaagaa aaaagtggtc
    43801 aagttcttgg aaccttagca actggttctg ttacaggaac attacttgga ccgcttcttg
    43861 gcgggatctc tgcttcagcg tttggctatc gccctacctt tttcattaca gggatcatct
    43921 tatttttcgt cttcttgttg agtctgtttt tcgtccatga aaaatttgtc ccagtgaaaa
    43981 aagaaaacat ggtttctgcc aaacagattt tcaaagactt gaaatatcca cacgtcgtca
    44041 tcggtatgtt cgtcacaacg atgatcatcc aagcatccaa caattccatt agtccaatca
    44101 tcagtctgta cattcgccaa ttgctgcatg gacatgggaa tgttacgttg atcagcggaa
    44161 tcattgcttc gatacctggg attgccactt tgatagccgc tccaagattt ggtcgcttag
    44221 gtgacaagat tgggagtgaa agaatacttg cgattggttt gggttttgcg attctggttt
    44281 atatcccaat ggcattcgtc acgaatgttt ggcaattagc tgccttacgt tttttgattg
    44341 gtatctctga tgcttgtctg ctccctgctg tccaagcatt gatcacaaaa tattcgcctc
    44401 atcaagctgc tggacggatc ttcagttata accaatcttt ccaagcaact ggtaacgtag
    44461 tagggcctat gatcggctcc agtgtttcca gtgcatttgg ttatcgtggc gtctttcttt
    44521 cgacttctct tcttgttttg gtcaacttct tgcttgtacg acacaataca caagagatcc
    44581 atcagcaaga aactgcagaa gaaaaagctt caggagtctc catagcttca gggcactctc
    44641 cttctcatag acaataagcg taaatgaaaa acactgcttg aacagttgtc acagactgtc
    44701 cttgcagtgt ttttgtttag gcctgttttt tcaagaactc agataagagg aataatccct
    44761 tttccaaggt ttctttttta caacagtaac ctagtcgggc atgcttcggt aaatcaaagg
    44821 cacttcctgg tactaatagt actcctgttt cttctaataa attgatacaa aatgtctggt
    44881 catcttcttc gatggctaat ttaatgaaag aagtagagac gtaattggga gcgacaagtt
    44941 caactttcgg ttcattggct acccattgtt ttaaaatcac aaggttctct gtaatgatct
    45001 ttttgtttcg cgccaatatt ttttctttat tcttcaatgc atgtacagca agatcatctg
    45061 acaggactcc gccgcaaatc atcgtgtagt ctcgatactt tcgaaaaact tctgccaact
    45121 caggtccagt agctgtccag ccaatacgaa tacctggaat ggagtaggtt ttggataggg
    45181 aattggttgc tatacctttt tcatagacat ctacgatcga caagaattca cctttatccg
    45241 ttaaaggcgc atatacttca tctatcaata catatgcatc tactgtacga gcaatttctg
    45301 cgattttttc taaagttttc cgatctagca atgtgcctgt aggattgttt gcactattca
    45361 aacagatgat ttttgtttca ggtttgacta aggcttctag ctgttctaaa tcaaactgcc
    45421 aatcttcttc ttccttcaaa tgaacaaagt caacagcggc acctaaagat ttggggatgt
    45481 catataattg ctgataagta ggaagcatcg aaacaacatg gtctcctgct tccactaacg
    45541 cgtatagagc taaaagatta gcaccagtcg ctccattcgt ttgtaagatg ttttccggat
    45601 cgacggtttg ataaagagaa gcgacttctt gtttgaaaac atctgatcct tcgatccagc
    45661 catagtccaa tggttcgttt ttgcgttttt cgaaaaattt acttacgcta gtcccatcta
    45721 agccaacgag ttcgtctaat gttaatgctt cgatcgagct ttgtgcaata tcatataccg
    45781 ctttcttttc ccatttattt agccattctt ctacaccgaa atttgcgatt ttcatgtttc
    45841 tttccccctt atatcttgat ccagcctaca gcaagcaacc cgattgaaag cagtgcggcc
    45901 acacaaatca gcccaatcat tactttttct tttcccgtaa tttgatgttt ggtttcacga
    45961 caagcgatcc agtagaagca aaaaccagga agataggcaa tactacataa aaggacataa
    46021 gaaaatcctg ctaaaatgat gcccatcaat tcaaaaacta cagcaatccc accgatcact
    46081 acttgttttt tgattccttc ttttaaagaa aactggactt gatagatccc gacaagtaga
    46141 taacaaatca aaattgccgc tgtacataaa gaataggcaa attgataggc ataatcagta
    46201 actaagaaag tcaataagaa tagattagtc aagattgcag taacaactaa cgaataagtt
    46261 ggggcatttt ttttattcgt ttttccccaa cctttcggca acagaccgtc tcgagccatc
    46321 aacatcgttg tttctgctgg gagcatcgtc caagacaacc aagaaccaat gatcgaaata
    46381 atcaagccga tattgatcag cgctgctccc caatttccca cgattccttt taaaatattt
    46441 gccatagcag gttgtgaaat agttgctaac tcttcttgag ataagacgcc gtatggcaag
    46501 atcgaagcaa ggatatagat aaacaataaa caaagcagac caatgatcga tgcttgctgg
    46561 gcttctgaac gtttcttcgc gcggttggct aaaacacttg cgccttcgat ccctacaaag
    46621 acccacatca taaccatcaa acaaccttta atctgcaacc agatcgttcc ttgctgccct
    46681 gttccattca ctagattatc agatacgtta ccccaaaaat cagcagtgaa tatattcact
    46741 ttaaatgcga taaatacaaa tattaaaaat aggaataatg gcactaattt acagatcgtc
    46801 acgattgtat tgatgacgct cgctccttca actccattgt ttactaataa agtcagaccc
    46861 caaataaaaa tacttgcaaa taaaatagaa ggaacatttt gtccaccttt gaatatcggg
    46921 aaaaattctc ccatggcgct catcagcatc gttgcaaaag caacatttcc cagccacgca
    46981 gacaaccagt aaccccaacc actgatgaag ccacctaatt taccaaatga agcttccgcg
    47041 taaccgaaaa taccaccatc tagatctgga cgcttttctc ctaaatgatt cagcgataat
    47101 accaaagcta aaataccaat cccaacgatt atccatgaaa ggatcgctgg ccctggagct
    47161 gcagaactag ccagatcact tgtgatccca aacacaccgc ttccgattga cgaactgaca
    47221 accagagcaa ccagtcccca tttgctaata cccgtcgatt tcttttccat ttccatcacc
    47281 ctcacttata aaattgaaca aatcacttgc aaaagattcc cttctatttt gcaactgctc
    47341 ttgcttaaca aaaagcaact ttattcatat tattggatat tatgaacttt ttacacaagt
    47401 atttcaagta taaaatgaat aaaaattaat tttattgcaa aaaaacagct atgctatcca
    47461 aaagaaaagg tgtttctttt gttagccata gctgttttga ttcatttaat atcgcttaaa
    47521 cgtattgtaa aacttttgtt tcttgatatg cgtggtcgat caaaccacca cccagacatt
    47581 ccattccatc ataaaataca actgcttgtc caggagtgat tgcacgtaca ggttcgtcga
    47641 atacgacttc tgcacgatta tcatccaata agcgaactgt cacaggaacg tcttgctgac
    47701 gataacggaa tttcgctgta cacttgaatt cttttggcat tggttcattc gtcgtaaagt
    47761 ggatttcact tgcatccaag cttgtggcat acaatgcagg atgatggaac ccttgcccca
    47821 catataatgt gtttgtcgct agatcttttc ctacaacaaa ccagggttct tgcgaatctc
    47881 ctccgccacc gatccctagt ccttgacgtt gaccaatcgt ataatacatc aagccagcat
    47941 gttgtccttt gacttcacca tcaagagtga ccatatttcc tttttttgct ggcagatagt
    48001 tgctcaaaaa ttgtttgaag ttcttttcac caataaagca gattcccgtt gaatcttttt
    48061 tcttagcagt cgcaaggcct gcgcgttcag caattgcccg tacttctgat ttttccatgc
    48121 cgcctagagg gaacatcgtt tttgaaagct gttcttgtga taattggcta aggaagtaag
    48181 tttgatcttt atcattgtct actccacgaa gcatgtggac cacgccgttt tcatcgcgag
    48241 tgacttgcgc ataatgtcca gtagctacat aatcggcgcc aagatccatt gcataatcca
    48301 agaatgcttt gaatttgatt tctttgttgc acatcacgtc tgggttaggt gtacgtcctg
    48361 cgcggtattc ggctaggaaa tattcaaata cacgatccca atattctttt tcaaaattca
    48421 cagagtaata aggaatcccg atttgagcag ctactttcgc tacatcctta taatcttccg
    48481 tggccgtgca tacaccgttt tcgtccgtgt catcccaatt tttcatgaat attccgatca
    48541 cgtcgtatcc ttgttccttt aacaaaagtg ctgttacaga tgagtcgaca ccgccgctca
    48601 tccctacgac aacgcgtgtt ttgctgttgt ctgtcatgat atcaccatca tttcaattag
    48661 attctgaaac atctacgagt tgaaggtcgc aacttttcag tattttacat tatacgtcat
    48721 atttctgaaa atttcatccc ttaattatca agtcgtaaaa atactgttct actggaccta
    48781 ttcagtacac aaacatgata gtcaaccaaa aaatcactca tcttttgagc attcgatata
    48841 aaaaattggc atactcataa aaagggccag cctttcggca ataaggaata attttataaa
    48901 ttgtgaaaaa ctatttttca aaattgcttc ttatcgctta aagctgcacg tctcatttac
    48961 aaccttttct ttttagatgt tatgagttgc tcagataagt gattttgttt actctaagaa
    49021 aaaagggagg caaaagatac atgaattact ttatcagtga tatgcacttt ttccatgaac
    49081 gcttattagg aaaaaacgac tttgctccgc ggctctttgc aaatgtggat gaaatgaacc
    49141 aacagttgat cactagctgg aatgcagtaa taaaagaaac cgaccatgtt tatcatctag
    49201 gcgaccttgc tatgcatccg caatatgaaa aagcaaatgc agaaattttg gaactggtca
    49261 ctcaactgca aggaacaatc cacttgatca aagggaacca tgacagtcgc gcttttttcc
    49321 gttatttaga aaaaaatgat cctaggctta ctgacggaag tcccaaattt tatttttatg
    49381 atgtaggtgc catcgttaaa ttcgaccatc aacaatatta cttgactcat tatccgatgt
    49441 tgctcgggcc taacgacact atccgcaatc ttcatggaca tatccatcat tacagcgtac
    49501 ctatcgagaa cgatgtcaat gtaggcgtag acgcaccgga aagagaactc ttaaaggaaa
    49561 aactaccatt tggcactccg ttatctgagc aacaaatcaa agaaatctat gaagcaaaga
    49621 ggaagtacga taggtcgtga aaaaactgcc ctatatcaag tattaaacaa ataaagataa
    49681 ttactcatat ttttcatttg agtaggctta ttgccacagg aacattagga tcagaaatgg
    49741 agttgtaaca agacttctgt cacaactcca ttccatacta ttcgtctttt tgctcatggt
    49801 gttcttttac cagttttttc tgtaattctt tatcataatt tcgggcagct gacgttaaaa
    49861 ataaaacgat tccgcataat aaaatcccga ttccggaaaa caagaacatt ttttctaccc
    49921 ctaaacgatc agctaatggt cctgcaaata ataatccaat tggacctgcc aggttcatca
    49981 aagagttgaa gttccctaag acacgaccta aaacattcga ttcataactc tgctggatca
    50041 ttgccatcag cagtgtattg aaatatggcg ttgcacaacc tgctcctgca ttcaagataa
    50101 gaaagtacaa gaacccttga ctatcgtttg gaaccaatcc gcttgcaccg atcgtcaggc
    50161 ccataatgat gtaggctata ataataggtt tcattcggtc tttccatgtt cctataaaac
    50221 tgatcagtgc accgcctaag agcattccga ctgcgtagac tacttccact gctcccgcct
    50281 gtccaacgga accaccaaag taatctagcg tcatcaatgg atacagactg attgctggca
    50341 tgaacaacag catcacaaat gcaccattga tcgttatgta ccataaccct ttattatcca
    50401 tcaattgctg cagcccaaat tttgcatctt taaaaatagt aagcggaact tccaatattt
    50461 caggagaaac ttttgggatc ttcacaaaaa tcaataagcc gacaccaaaa actgccccca
    50521 atacatctaa taaaataagg taattgacag gaacgactga aaacaaagcc gcacctagag
    50581 ctggcgcaat gatgaaattc gctgattgga ccattcctaa ctgtccattc gtccttgtta
    50641 tgtgagaaga tggaacgatt gtcggcataa tcgactggat cgttggcatt tggaatgttt
    50701 gtgcgaccga acgcataaac aaagacacaa aaactagcca aatcgggaag gattctgaaa
    50761 tcgttccgac tactgctaaa ataagcgcga aaatcgctac aataatatcc gtgacaatca
    50821 aaagagcttt tttatcccag cgatccacta gcggtcctac aaatgggctt agtaaaacca
    50881 tgggaatcat tcctagtaaa gtagcaaaac tcaaaattgt cgccgaaccg gtttctcttg
    50941 tcaaatacca gatgatcgca tattggacga ccatactagt gattcctgac aaaaactgtc
    51001 ccgataaaaa gaacaaataa tttctcttcc aattcgttaa aacgaccgac tgttcatttt
    51061 ccataaacat ccctcttttc tttttcattt tgtatagcgt ttgcacaaca atcattatac
    51121 gggattcttt tttcaaaaaa tacctttttc cctaaataac aatcgtttca atttaaattt
    51181 ctttacttag aaacaataaa tcagcaaaat attaaaaata acaacatgtg ttaaatttaa
    51241 aaataaaaaa gacaactaat ttgcgtcgtc cttacaaagt tagtcatcta acaataggcc
    51301 gtttcaaaac tagttgtctt tttatatcca cagatcattc gactttttcg aataacccac
    51361 ggctaatcat cccatccatc agaaaataaa atttatcttg gctcattttc tgttttggat
    51421 ctttgaaaat attgactgct ttcaatccat ttgctgtagt gatcgacatt ttcaatgttt
    51481 gaatatcttt tgatacagtg atttttaatt tgaatccttc tttgctttgc ggcgtggcat
    51541 ctaatggtcg gatcaattga taattgcctc cgtttgtttc tgtaaatcca ttatcgcgca
    51601 atgtatatct ttttgcatca ggatgcaaac gatagctaat tggtgcgccc agtaccgttg
    51661 ctgttgtttc aaatgccatg attttcacct cgataaaatt agtttctctt tattataaaa
    51721 gtttatcctg atttttacta ggtttttcag ttaagttccc taaaaaatga aggcgttctc
    51781 tcagacatct gttcatataa gaatgacttc actagttatt ttttgacagc acgaactaat
    51841 tgatcaagga aatactggat gtcgtcttcg gtatttccca aaccaaagct gatacggatg
    51901 gattctttta gtatcggtgt atcttctcca tacattgctt ctaatacgtg tgaaggctcg
    51961 atatttcctg ctgtacaagc tgaaccagta gaaatagcga ccccattaag atcaagcttc
    52021 atcagtagca aatcatttgc ttcgccaatt aagcgaagat tcaacacatg agggagttta
    52081 ttcgttaaat caccatttac ttgataaggg atttcgtttg cttctaatgt atcaagaatc
    52141 aattgctgga atcctttata tttgttgtta cgtgcttcac gttcttcagg tgacagcagt
    52201 tcaactgctt ttgccatacc ccagatccct gctaaatttt ctgtacctgc acgccgcttt
    52261 tcttcctgct ccccgcctaa aagcaatgca gggatattta ctccgtctcg tttatataag
    52321 aaaccgacgc cttttggccc attgatttta tgagcagaga tacttaataa atcgattcct
    52381 aattcttctg gatggatcgt ttggtttccg tacgcttgta ccgcatctgt atggaaataa
    52441 gctggatgat ccgctaaaag ttcaccgatt tctttgattg gcatcaaatt tccgatttca
    52501 ttgttcccgt acatcacaga gaccaagatc gtatcttctc gtaacgcttt tttgacctct
    52561 tcgatcgaca gattcccttt ttcatctaca ggcagatatg taacgtcaaa cccctttgtc
    52621 tctaaatatt tcatggtatt aagaacagca ggatgttcga tagctgtagt gattagatgt
    52681 ctgccttctt tttgacgcga gaacgctgtt tctaagatag ctgtattatc cccttctgtt
    52741 cccccactgt taaagatgat ctcgtgaggt tttgcttgca gactgtctgc aatgatttgt
    52801 cttgctgttt ccaatttttc atgcgcgtgg cgaccaaatc catgtatgct tgaaggattt
    52861 ccaaacgtgt cttgcatgat tgccgtcatc gcttcaatta cttgtggatg catgggggta
    52921 gttgccgcat gatccagata aatagttttc acttcattca tcccttccaa attattgcta
    52981 ggcacattct agcataaaat agcgaacaaa ccaaagaaga gctcattttt gtctagagga
    53041 cgtttcatgg gcatagaaaa aaggagctgt gacaaaagtc ttgtcgcagc tccttgaagc
    53101 tgctcacttc tgtcacaact tctttccctc ttttttttgt ttattcgaat tctttttctt
    53161 ccagacggaa taacggactc atcggtgtat tttcatggat gcgtttgacc gcttcaccca
    53221 ttaattctga acatgtaacg attgttaagt tttctggatg acgattttct tctgtgaaaa
    53281 cagaatcagt gatacaaata tctttgattg gtgcattgtc taaaatactt ttcgcatgat
    53341 cggacaataa tccatgtgac gcacaggcat atacttcttt tgcgccgttt tcttttaata
    53401 catttgctgc tcgtgcaagc gttgctcctg tgttcaagat atcatctacc atgatacatg
    53461 tctttccttg aacatcaccg atcacataac caccctcagt tgaagcatca tctgcatgat
    53521 ctacgatggc taatgtgctg ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc
    53581 tgttttttgg cgaaacaact acgatgtcat ctcccgtcag acctaattga cggtaataat
    53641 gagcaaacaa cggcatcgta aataaattat ctacaggaat atcaaagaat ccttgtactt
    53701 gaaccgtgtg cagatctaaa gtcagtactc gagttgcccc tgcttcttcg atcaagttag
    53761 cgatcaattt cgcagtgatc ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc
    53821 catagtaagg caatacgaca ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca
    53881 tgatcagtag ttccatatag taatcattga caggtgcatt cgttgattga acgatataca
    53941 catgatcccc tcgaatactt tcttcgatat tgatggaaat ctcgccatca ctaaattgct
    54001 tcacgacgct tttacctaat tctgttccga acactttcgc gattttttca gctaatggac
    54061 ggttgccatt caaactaaaa atcttcaacg tgtcatcctg atacttatta ctcatgtagt
    54121 cctccccaaa acttctctat tcttcgatag tttaacatat ttttccaaga ttttctgtta
    54181 atttcagcaa attcttaagg aagtgaaact gttccgttac tccaactggc aggaatgact
    54241 ttatgataat cgccccagtt gtcgtgtact ttgtaataac gttctcctgt ttcagtttca
    54301 aaataagcgt acgctgtcac ccaatgattg ccatatgtac tgccaagtac tttcaaaata
    54361 ccaatcatca caggtttgcc ttcacgtata cgttttgtag ctcgctgcca tgaacctaga
    54421 agagttgctc gtgcacgctc atgattgcca ttcttcttta aaaaactaga aatccctgta
    54481 ctaacctgaa aaggaatcgt aggaagtcct agtggttgga tctggctacg caatgccttc
    54541 gtcaaaacca acgaatcagc cgtatttttc ttgcgaatct catttggaag catccacccc
    54601 ttacgatagt cttgataata ggcaaggaga actgctgccg cgtaagtacc acaaagatag
    54661 cctgaggggg tagcccattt cttatacctc aaaaaacgtt cttctggcaa tcccacccag
    54721 caatttgcga gttccttttc tgaccttttt tcttcgatca aaacgggaat atcttctttt
    54781 ctgttgatcc atctagataa ctcgattggc tcttcattga tcagcttcca ttttttacaa
    54841 gaaagaccct cttgataagt atattttgct ggagaatatt ctaccggatg tcctgattga
    54901 tcgataatca cataactttg atcttcaaaa accagagcta aaaaaagcaa tttacttttt
    54961 cttgactgca gctgctgagt gctggctaat tttttaattt ttttatctac tttgaaatat
    55021 aaaggtttat ccactacttg attcagttgg gatcgcctcc tttttttcat tataagtgaa
    55081 ggattgaaaa aaacaaatca gtttgattgt tcgaattttc aattttttat gttctatttg
    55141 ttaattcctt cattttgtaa gataatgggc acacgaaaca aaaaaggagc gatcgatgaa
    55201 tgaacaaaga acgattgaaa caagatttct atgaagcagt caatgaagaa tggcaaaaaa
    55261 cagcaaaaat ccctgcagat aaaccagcta caggcggttt ccaagattta gttgacggga
    55321 tcgaccaact tttgatggga gaaacagacg atctgcttgc ccatccagaa aaagtgtctt
    55381 cagaaaaaat gaaacatttc cttgcatatt atcaactggc aaacgattat gaccgaagaa
    55441 atgagttgca tgctcaaccg cttcaaccta taatcgaaaa agttgaatca ttgcagtctt
    55501 tcgacgaatt gaataagcaa tttccagaat ggctgttgga tacattacca ttgccattta
    55561 atctggatgt agatgcagat atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc
    55621 cttctttatt ccttccagat aagacgtatt atgcatcaga ccatcccaac ggtgcacaac
    55681 taataggcgt tttctttgat atggctgttc agttattaga gcttgccggc aaagaaaaaa
    55741 gtcaggcaga agcaatcacg gaacaagcag tgcaatttga taaattgatt gctcctcatg
    55801 taaaaagtgc agaagaaaac gcagattaca gcaaaatgta taatccgcaa tcttttgata
    55861 catttattaa atacacagat aaactggact tgaaaaaact tgtcacgggt ttgattggga
    55921 caactccaga taaagtgatc gtaactgatc cagtgtactt cgatcatttg aatgacatcc
    55981 tgacagaagc tcattttccg ttattcaaaa gctggatgat cattaaaaca gtacgttctc
    56041 ttagtggtta tctatcagaa gaattccgcc aagtcagcgg aatctattct cgcgtattgt
    56101 ctggaacaga cgaagcaatg cctcagaaaa aagcagcgta ttaccttgcc tctggacaat
    56161 tcgatcaagt agttggcgat tactacggaa aaaaatattt tggcgaagct gctaaaaaag
    56221 acgtagaaca aatggtgaaa aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg
    56281 actggcttgg ggatgcaaca aaagaaaaag ccgtcataaa attaaataac ttaggtatcc
    56341 aagtaggcta ccccgataag atcccagcat tattcgacca gttcaaaaca gtacctgcag
    56401 atcagggtgg tactttgctt tcaaatactc ttgttttcag caaactcaca ttaaaagatc
    56461 gtttcagcaa atggaacaaa ccagtggatc gttcagaatg ggaaatgagt gctgacacgg
    56521 tcaatgctta ttatcatcca tttcggaatg tgatcgtctt tccggcagcg atccttcaag
    56581 caccatttta cagtctagaa caatcaagct ctgctaatta tggtggaatc ggagctgtta
    56641 tcgctcatga gatttcccat gcatttgata ataacggcgc tttatttgac gaatatggca
    56701 acttgaacaa ctggtggaca gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa
    56761 tgatcgaaga attcgatggt ctgccttttg ctgacggaaa agtaaacgga aaattaaccg
    56821 tttcggaaaa tattgctgat gcaggcggtc taagctgtgc attggaagca gcaaaaacgg
    56881 aatcagatct ttctatcgag gaattctttg tcaattgggc aacgatttgg cgtacaaaag
    56941 caaaaaaaga ataccaacaa cttttactgc aaatcgatgt ccatgcccct gctaaactcc
    57001 gagcaaacgt gcaaccaaaa aaccttgacg aattctatga aacatttggt atcacggaac
    57061 aagacgctat gtatctagcg ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag
    57121 aagtggtcag cttcaagaaa taagaaggaa tttgggaaaa tcgcttctca aatttttgtg
    57181 acatttcggc ttatttttcg aagaagttac ttctggaata ccatgtatta gaggttgtga
    57241 aaaagctgct tagtttcaag tagtaagaaa aattttgtga aaatggcttt ccacattttt
    57301 gcataaattt gacttactac cgagaaactg gcttttgaac accgtttatc gagaggttgt
    57361 gaaatcagcg tcctgacctg agtattagag aaaaaaacgg aaaaatagtt cctcatattt
    57421 tgccgtttgt tgagctaata ccgagggtca gctgattgaa caccgtttag tggagagatt
    57481 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaagcag caaggagaac
    57541 ctcatctata aaaagataga aggaagtgag acatctattg gtctcatttc cttctatttt
    57601 tactttcctc agtcaattgt tttacatcat aaaaggagaa attccactta tttttttcgc
    57661 tgtttcatga cataaacaac tacaccgatt aacaaaattc ctccagctaa tgctagcaac
    57721 gggttattaa attctcccgt ttttgggagt ccttgagtgc catttttttc actttctttt
    57781 gctgtcctat ccggatgaga aggttttcca tttggttttc catttggtga ttcatttcct
    57841 ggattttctc tttctggttt gtcatttccc gggggcacat cggcagtttc tcgcacagtg
    57901 atcgcttttt gctgggtaac agattctgta tgagcagacc atttttcccc atcaaatatt
    57961 tccgaacgat aagtgacttc tagcgtatat tctccttttg aaggtgctga aaaaatagcg
    58021 gtatgattag aaaattgagc acttgactgt gtttcaacga tttggatctt ttctgggaaa
    58081 taacgcatat cttcaaagac aggcgttcct tgatttggtg tgaaagttga cacaaaaact
    58141 gtaaaaggct tcagttcaat gatatttcct tctgcattct ccgccacacc atttgcttct
    58201 tgtataataa tcggattatt ttgttcattt ggttttgtct gatttggttc ttgtacgatt
    58261 gcatactctg gatattccga agacggtgag gtttctccgt caaaaatagc acctacagat
    58321 aatggaagac catccactct ttccttttct gtttgacgat tccccggttt aatctgcatc
    58381 agtccatgaa catggatact tccattttca tagcgcgtaa caggctgctg accttctttc
    58441 gaacatgtat ttagagactc gatccaatct tttgatatgg tatgtccaag agtattttct
    58501 gattgtttct ctcgttcatt ccaatagtag ttcaacgagt ggtagatttg atcttcgttt
    58561 tgattcaatg ggcggatctg ctctctcatc tcaagattct ctgttcggaa agataagata
    58621 ccagaagcat ggtagtctgt gtttcctgct gaagaggtat aaagagccac attatatgaa
    58681 ccgttattaa aacttgtact gctattgacg ataatatctg ttccgctatt ggaatcgatt
    58741 cctttcgcaa gattttcgaa agacaaacta ttgatcaatt catgagggcc cgttaaagaa
    58801 gaaccgccca ttttgaatcc attaccgtta ccttctccct caattcctgg tacccagccg
    58861 tttcgataag caactgagtt tttgatgatt acttttccga tcgaacctgt ttcattttta
    58921 gcaaaaaggt cccaaccgtc atctgcatta tgatacgcga tacaaccgtc aaatacattt
    58981 ccttctccta ttgtcagttt ggccgcaaat ccatcggcat cttcaaatcc tggatcggca
    59041 ttttcaaaag atgtacagtt cttgactaag ttatgcgcgg gccatgcttc gaaaggatca
    59101 tttgagctac cgctgatttg gattcctgta tttccattcc gataagcatg aatctcttcg
    59161 atcaaattat gatttccaga aatctgtaat cctttttgca tagctgcact atttgtaatg
    59221 tcaaaccctc gtaaacccca gtagtgactg aacagtgtca tcccatttcc agttccttta
    59281 aaatcaagta caggacgctt gttttctgga tttttctctg caatcaactg aatcgggttt
    59341 tctcttgtcc cgttaacgcc ttttggaatc gtcaatcctc tttcgaagga ataagttcca
    59401 ggcgccatga cgattgtctg cccagcatat gcgtatctca atgcttgtgt cagagataat
    59461 ggattttgtc tgcttccgtt tcctgcatct gacccttctg gtgttacata aactgtcgaa
    59521 tatttaggtt tacggtagtc cactgtatga agaatctctt ttgtttcata actagtcatc
    59581 tctacgtatt cttctggcgg atagttttta tctggcgtaa aactgaaagt aaattcgttc
    59641 gttccttcca tcagttttgt ctgataatca aattcccgat tcgcagtgat agccaagttt
    59701 tttgcgttct taagcacctc tcctgttgct ttatttttaa ttgtcaataa tccgtcagca
    59761 tttgctctaa aggacatagt atgatcttgg tttcctgaat gattactaga ggtcacagtg
    59821 taaatcggat caatcaagcg agtaggtctt tcttgcccag gaggatctgt cgcaggatct
    59881 gagtaagcaa cttcgattcc ttctaccttg atcgtcatat tccttgctgc agcaaatcct
    59941 gcataaataa ccgattcatc tgaagcgtat aatttttccc agtcatacat gatgtcagaa
    60001 gccatttctt ctcctgtttc aggatcgtaa taatatgcct ctaaaccagt attcgttttt
    60061 ttcatctcta cgtccagttt agtaatcgag ttcgtggccg gaaacagatt cccatgaccg
    60121 tttccgaata tattataaga acctgtttct ttttccaaga accctgctga agtctctaaa
    60181 ggtgttgttt ccactgagac actcccagga gctggcgcag cttgcggatc ttcagatgat
    60241 attcctgtga ttgcccgtgt ccccaatcca agtcgcatcg tatatttgct gccgctcgtg
    60301 ttgaccactt cttgtctatc cgagtcccag acatattcga tccgactgcc tagtatggca
    60361 aatgaatttg aataaaatgc ctgcccaaac aaatgatttt ttggtactgc gtctcttacc
    60421 attaaagcaa atccttcttg ggcattcgta taggtccaag attctatttc gatcgtcgcc
    60481 cgcatcctaa agttgatgtc tttggggatt gcttgataat aataggtcat tccatctgat
    60541 ccgcttgact ggaattttcc gccgttattc gtggaagaga gccgcacgcc gtcttctaat
    60601 ggttcaatgg catacctact agcacttgtg ctttctccga aaacaacccc tttccactct
    60661 tcatttgctt cttcagattg cttttttgtc ttttcatttt ctgattcttt catcatagga
    60721 tcatttcctt cttccgtatc catatcttca ctagttcttg gtatttcctc cattgttgac
    60781 atcccttcaa tgttcacgtg tgcattttct tcgttcacag actgttgttc gttagcagca
    60841 acttcaacga cagaaattaa aaccggggta ctgagcataa ggagattcat cattagcgca
    60901 ctgtatttta tacgtttttt taacaagtgt attcctcccg taacaaatta gaaagcgttt
    60961 acttaaagta ttacctaatt tatttttact gtcaataaaa aacaaaatta taataagtgt
    61021 attttataaa aagaaaaaat ataacttaag ttgttgataa taaagagatt tattaacgta
    61081 aaaagaataa gtttttttaa aataacgtat aaaaaagagc ataaaacaaa gtcgtttgtt
    61141 ttatgctctt cttttattat tttgtgcttt ttattttatc aaatcacttt gtttttttat
    61201 aaaatttctg cgcctagtgt ggactcaaaa tgtctcaatg cccattcatg tccaacggga
    61261 tcgaaacttg ccacggcatc tttgaaaata tggagtttgt atcctagatt atacgcatcg
    61321 actgctgtat gcaacacaca gatatctgta catacgccgg tcaaataaat atctgtgatc
    61381 tggcgttctc gcaaacggat gtctagatct gttccactaa aagcggaata atgccgtttg
    61441 tctatccaat aaacattttc ctcttgctca tagtcttggt ataaactttt taaagagccg
    61501 tacaattccc gtccacccgt tcctatcaca ttatgcggag gaaataagcg gttctccgga
    61561 tgaaaggaat cttttggatc atgcgcatca atggcaaaaa cgacaaaatc cttttgatca
    61621 ataaacttct ttgtatgagc agtcagcttt ttttcaatta cttgtcctgc tgtcccagta
    61681 gtcagctttc catcctctgc aacaaaatca tatgtgtagt caatcgagat caatgcgttc
    61741 atcttagtcc tccttatacg agatgtttga caaattcgat caccatttct gctgatcgtt
    61801 tgcctgcgtc ttcgataaat tcgtcaaagc tttgtgtcgc cgagtgatct gctgtgtcgc
    61861 tcattgcacg tacgatcaaa aatgggatat tgaactgacg agctgtttgt ccaacagcgg
    61921 ctccttccat ttcgcaagct aatgcttcag ggaaattcgt taagatttct ttgattttat
    61981 ctggcgagtc gacaaatgta tcacctgtaa cgatcaatcc ttcttttgcg ttcaaattcg
    62041 ttgcttttgc tgctttaacc atttcactgc gcaaatatgt gcttgcttca taatatagag
    62101 gcattcctgg taattgtcct gggttgtagc caaatcctgt tgcatctgca tcaaaataag
    62161 ctactttgtc tgatataacg atgtcaccta cttgtaagcc ttcaccgatt ccgccagctg
    62221 aacctgtatt gatcaccatg ttcacgccgt attgttggat caacagactt gtggtaatag
    62281 atgctaagac ttttcctata ccggaacgta cgacgatcac ttcgtggtta cctaatgaac
    62341 ctgaaataaa taaagcgcct gcgcgttccc atgataatgg ttcgcttagg ttctctctca
    62401 aaatttttac ttcttcttcc attgcgccga taataccgat tttcatcttt ttaattcctt
    62461 ctttctatat aaatgcgatt gctaaaaaaa ccacacataa cattaggata acaataataa
    62521 gagatttcat caaaaaagag ttccatttgg tcgtcttttc ccgttcgctg atccttgttt
    62581 tcgtgattgg tttattcttt ttgctttctt tacgataaaa gctggcaatc tttttttctt
    62641 cttgctgata agctgtttct gcttttcgct gtttttttaa tgactcgctt gcttgtgctt
    62701 gttgtcgctt gcgaagttcg cttcgcgtaa taagcggtcc tttggccata agccgatccc
    62761 tctactttcc tttggtcaat agttcccagt attgaatcgc ataaatcgtc tttgcatcac
    62821 aaatcaactg atctttcatc gcttgttgcg cctcttcaag agttagatga tacaactcca
    62881 gtacttcgtc ttcatcttgt gctaaaggat tctcgacctt ctctacccct tgtgcatgat
    62941 aaatatgaag gacttcattt gcaaaacctg gtgaaagata catcgaagta agatggctca
    63001 gcgacttcgc acgatagcca gtttcttctt ctaattctct agctgctgtc atttcgggat
    63061 tttgtccttc tcctggatcg atttttccag ctggaatctc caaaatcact ttttctaaag
    63121 gttttcgaaa ttgtttgaca agaagcagac gatcttgttc gtcaaaagca atgatcccga
    63181 caccgccagg atgaaatacc agttctctct tcgaagttcc tccatctggc aaacgaactt
    63241 cgtctacagc aacatcaata atttttccgc tatagatttc tttgcgactg attgtttttt
    63301 cttcaaattg ctcattgttc agcatatctt ctcctgcttt ctttgtaatc ataactgctt
    63361 ttttttcatt tttcaatttt tctcaaaaac tccttattag tttaccatag ataaacctta
    63421 gattttctct aaatatagaa aatccttctt tagtccgata ctctttatag tagtccgtat
    63481 gctaaaataa gtatgtaatc gaataacgaa aagaattcaa tactcaacga taaatgacat
    63541 ggagggttta ttatgataaa acgaaattgg ccgtggcttc tagcattggt cttgctattt
    63601 ctctttgtcc gaaacaatag tttttctttt gttctaggac ttttattgat aggtgtagtt
    63661 ttagttttgt ggggactctt tggttcgcgc aggaaaaagg gaaaacaaga agaaattcct
    63721 gcactgactg acgaacttga aactcactat gaaaaaagcg gtatgacagc gaatgaaatt
    63781 tcattttttc gacaaacaat gaatcaaaca aaaaatgaga tcaatcaatt gcaaatgaat
    63841 atgcagcaga cagccaaact taaagcaatc gatctccgcc atgatccagt caaagcagcg
    63901 aaagctttat tcaaagaatt ggtgaaagaa ccaaaccgac tgcatgaagc aagtcagttt
    63961 ctttatacgc atctgccgaa tctagttgat ttgacaaata agtataatga gatcaatgac
    64021 catgagatca aaaacaaaca gacctatgaa aaactagaag aaagcgcgca gatcatcgac
    64081 cagctgtcag ctttaatcgc acaagattat caaaaattcg ttgctgagga tctagatgat
    64141 atcgacgtag agatatctgt tgcgaaacag agcttaaagc gtgataataa atatgaacca
    64201 gaacaaaagg attaactctt ttgttcataa aagaagactc ggaacattca gatgatgtct
    64261 ggctgtttcg agtacttttt ctaaagtaga gacattcatg taagaatcga gaacagattc
    64321 aacaaggagg aattttttat ggatcataaa ccgactgaaa caaaagatgt tacaccagtt
    64381 aacgatacac ttgaagattt attgaacaac ccgttttcaa ctccaacaga tacgctgacc
    64441 acttcacaac aagcggagat tgatcagctc caagagcaac agacagctgc tcgtttaatt
    64501 gacaaactac cggcggaacg ccaagaacaa gctaaacagc tagcagcaaa aatcgatgct
    64561 aatgatgcac agtctgtcat cagctacgga tcggctgccc aagcaaaatt aagtgaattc
    64621 tcacaatcga tgttgaatca tgtgcaagca caagatattg gtcctgtagg agattcctta
    64681 actgaactga tgtatcgtct ccaagaagcc aatcctgacg aactgcgtgc cggagaaggg
    64741 aacatcttcc agcgtgtctt cgggaaggtc aaacaatcga tctatgaagt gacagcaaaa
    64801 tatcaaaaga ttggtgcaca aattgacaag atttcagtga aattagataa agaaaaagac
    64861 ggtctgctaa aagataatct gatgttagaa cagctttacc agaaaaacaa agactacttt
    64921 gatgcactga atatttatat cgctgctgga gaattgaaaa tggaagaact tcaaacaacg
    64981 atcattccag aagcgatgaa aagagcagaa gaaactggcg atcagatgga tgttcagatt
    65041 gccaatgatt acacgcaatt cctagatcgt ctggataaac gtacgcatga tcttcgctta
    65101 gctagacaga tcacgatcca gcaagcacca caaatccgtt tgatccaaaa cacaaatcag
    65161 gccttggctg aaaagatcca agcatctatc gctacagcca ttccgctgtg gaaaaaccaa
    65221 gtcgtgatcg cattgacctt gcttcgtcaa aaagatgcag taactgctca acgccaagtc
    65281 tctgaaacaa caaacgactt attgaagaaa aattcagaaa tgctgaaaat ctcagccatc
    65341 gaaacagcga aagaaaatga acgtggaatc gtggatatcg agacccttca aaccactcaa
    65401 aatgatttga tcgaaacaat ccaagagaca ttgcgtatcc aaaaagaagg aaaagaaaaa
    65461 cgtcgtcatg cagaagtaga gcttggacac atggaagaag atctgaaaca aaaactgttg
    65521 gatttaaccc aatagatcac ctttcgcttg ttactaaaac aaacgcccaa caacagttta
    65581 ttacaactgt tgatgggcgt ttttcttatc tttcttctaa cttctccatt ggagtctatt
    65641 tctcattcat cttgctgtgt ccatagccat agccaaaata aatgatcagt ccaataacca
    65701 ttacgatacc gaacacgatc catgtcacag tttgcagacg aagcatcaat acaatacaag
    65761 tcaagatgga gacgattggc aatagcggaa cgaatggcac tttgaattct ccagcttttg
    65821 gtttgccaaa catttttctc aacttcagta agcctagact aacaagcatc aaataggcga
    65881 gcgccatgat attcgttagt tccgctaata cattcaatgg gaaaaatccg gcaaagaata
    65941 cagctgccac accagcggca taagtcgctt ttttcggtgt acgatgcttt tgatcgattt
    66001 ctgtcataaa cttaggaagt aagccatcct tgctgacagc aaacagtaaa cgagcaagtg
    66061 aatacatcat cgagatcgtt accgtcagta gcgttaaaat tgcccctaca gcgatcaccg
    66121 cgcccactgt tccatgtcct acatacctca ttgcaaaagc cactgggtca ttgactccta
    66181 gttcagtata cggaacgatt cctgtcaaga tcagcgtcac gatgatgtaa aggacagtag
    66241 caatcagtat cgaaccaatg attcctcttg gcacatcttt ttgcgggttt ttgacttcct
    66301 cggctgccat tgcaacggca tcaaatccca agaaagcaaa aaacacaaga gctgcaccat
    66361 ctagcacacc ggaaaaacca aaaggcatga atggctgcca gttatcaggt ttaacgaaaa
    66421 atattcctac aatgataaac aacgcgatga taccaaattt gacatatacc atggcattat
    66481 tcaaacgtaa tgctttcttg gcttcaagcg acacgatgta agtgacgaat atcactacga
    66541 gcaccgcaat caagtcaatg tatgtaccat tttccggatt gtacccgcca gttaatgctt
    66601 gtggaagttc ggcccctaaa cttttcaaaa aaccttgcat atagccagac catccggaag
    66661 caacagagga aaccgctagt aaaaattccc cgatcaacaa ccaccctgtc agccaggcag
    66721 caaattcacc aaatacaaca tacagatagg catatggccc accaatcact ggcactcgcg
    66781 aagcaaattc tgcgaaactt agtccagaca aaatgacaac gattgcagct aagacaaatg
    66841 aaagtgacag tgctggacca gcattttgat tggccgcaac ccctgtcacc acaaatattc
    66901 ccgtaccgat gatcgcacca attccaagca tgatcaaatc agacgttttt agttcttttt
    66961 tcatctcact cgtatgattg agattgatct ctttcttacg aaacaaatcc attgaagttc
    67021 ctccccattt taggttggaa aacagcgatt aaactgtttt catataccct aaaaggatac
    67081 cacctaaaat gactaaaagg caaccaacaa taacataaat gagctctttt ttcgtttttc
    67141 tttcgccaag gatgaaaatc ccacctaaag tagagataat gatccccatt tgtgaaagtg
    67201 agaagccaac agctaaacca agtgatttga ctgttagaag catgcagatg tttcctaagc
    67261 cccataataa tccacagatc atgtttttcc acacaaactg atcgacttta actttacgga
    67321 aagcaaagaa gcttgcgcct aagatcattc cgatactttg aggtaagata attgacatcg
    67381 catccaaatt cgcccagttg acgatgatgg tatatacacc ataaccaatc gtagagaaga
    67441 tcaatgctct aaaaccctta ccaaaatcaa gcattttatt atctgtatct atttttcctt
    67501 cgtcatcttg tcgggcagtc aaatacgcac cgcttacaag aagagctaaa gcaataatac
    67561 cgtatacata atctttcgtt tgtgtccatt catggaagaa aacggctccc gcaatcgtat
    67621 tcaaaattaa ttgaaaacca gtagacaatg gtagccctac agaaacaccc atgtacttca
    67681 ttccatggaa ctgtccattt tgtccaacac tccaagctaa gccggataaa aatccgatca
    67741 agaaaatcca aggggtgatt acaggactaa caacgaaaaa gacaactaaa gaaaacagaa
    67801 atgcaccgat cgtcattcct agtgtttgtt gattggcatc gccaccaatt ttaccactta
    67861 ctaaaccaat actaccccat gccaacatag ggactaatgc tattaaaatt tccataacca
    67921 atctcctttt atttcacatg ataagaaact tatcagcgaa aacgttaaca acacaagcac
    67981 tttttttcgt ttctaacgat tttctaacgt ttttcattat aaaaatttaa gattgggaaa
    68041 aataaagtca ttgttgtccc ttttccttca gttgattcag ccgtcaaatg gatacctaat
    68101 ttttcggcca gattttttgc taaataaagt cctaatcctg ttgaatgtgt tttgctaagt
    68161 cgaccatttc tcccagtaaa tcccttatca aagatccgtt tcaagtcttc cgctgggata
    68221 ccgatccctg aatctttaac tgaaaggctg actccttgcg cttccttttc tatgctcaca
    68281 atgatgtttc cgtgatccgg cgtatatttg atggcattac taagcaattg gttgaagata
    68341 aaagcgatcc atttagcatc ggtcaaaaca gtatgatcct caccaacgat ctcgtagcga
    68401 atattttttt ggataaaata atttgcctga ctgcgtatca caggctgaat gattcctttt
    68461 agtccatatt cttggatgag gtagtcccgc gagaaactgt cgagtcttgc ataatacaaa
    68521 acttgttcta cgtattcatc gatttttgat aattcatttt ctagtaatgt atacttttga
    68581 tcgtcaatat cgaactcgat cgaacgaact agcaatttag aagcggaaag tggcactttg
    68641 atttcatgga cccatgagtc aatataatct ttttggtctt gttgattact gattgctgat
    68701 tgcattactt cttggtgttc acggatcagc tgattgatat attcttctac cagtttttct
    68761 tcttctgtcc tcccaccact caaataattt tgcaaagggg aagttttttg tatgtcggct
    68821 aattttcccc accatcttct cttgctcaag taaccaccca gcagataaaa aatcagaaag
    68881 acgccctcaa tcaaagctaa ataagcaatt gtgctcaaat caactttgat gtttggcgcc
    68941 agccacatca caaagatcgt caatgcaata aaaaacagcc atcccaagat caatgtccac
    69001 tgatctttca aatatttaaa aaaattcatc ttaaccgctc atcctttcag ggaacgatat
    69061 atccctgtcc tacttttgtt tcgatatatc cttggatacc tgcctgttcg atttttcggc
    69121 gtaaccgatt gatgttgact gtcaatgtat tgtcatctac aaatcgttcg tcatcccaca
    69181 gtgctcgcag caatttttcc cgacttaaga tctttccatt gtgcttcatc aaaataaata
    69241 atagacgata ttcatttttg ctcaaatcaa tgatctctcc gttgatttcc atacttccgt
    69301 tgtcaacatt caacgtaatg ccattgtggc tcatcatttc actagataat tctgtgtagt
    69361 tgtaagaacg gcgaagtaat gcattgattt ttgcaatcag tacatcgatt tggaaatgtt
    69421 ttgtcacaaa atcatctgcc cccatgttca tagccatgat catatccata ttcgtattcc
    69481 tgctggagat aaaaatgatc ggtactttcg atacttcacg tattttttga caccaataat
    69541 agccatccaa aacgggaaga ttaatatcta gtaatacaag ctgtggttct tctctttcaa
    69601 agtctgagaa cacttgattg aaatcggtcg ttccaaaggt ttcaaattgc catttctcaa
    69661 gttcctccat gatcaattgt cgaatgatct cttcgtcttc gactaccatg atttttgcca
    69721 taaaaaatcc ctccatcttt cttcttgcta ctacaatatc aaagaagccc ttgtgaaaca
    69781 agttggctta cctggaaaat gtcattttag caaacgttaa aaaaaactgg aaatatgcga
    69841 tttaatcgcg tttgtctctt ccaagcagct taaatttatt atgttattct taataattgg
    69901 tctattatct gttatttttc tttcgttgtt tttgtctttt aaattttcga taacaccaat
    69961 aaccgaaaag cgttagcact agcaatatac ctagaaggag tataagtaat cgataaagat
    70021 ggtatttttt tgtctgttct atctttgaag agacttcttc tgtaaaggga actcgtcttc
    70081 ctgtcactag taatcggtga gtgttcacgg tgtaaggtgt acaagtgata agtgtgacta
    70141 gatcctgctt ttctctaatt gctaatgtat ttgtttttgt tggaaggaca acaattttct
    70201 caacgacttc ataagcgagc atccgaccat acacttcaag atagaatcgg tcatcttgct
    70261 ccatttcttc aagatccgta aagagttttt tctcagcaat accgctatgg gcggtaatca
    70321 cggaatgtgt actctctcct ccagttggaa aagaggtgcc ttgcaggact gtcgctcctc
    70381 tatctaacaa aagatcattc gtttcatcaa ataaaggcaa gctaacattg attttcggaa
    70441 tatagatggc tccgatcata tgttgttcgt aatattcttt tcctggattt tggacatttc
    70501 taaccgcttg ctcaaaagga tctttgactt gtcccattcc tgggattact gatgtatgag
    70561 caagcgtctg atttttcttt tcatgcactg ctaattgttg ctcatgtttt tcttctttat
    70621 cgcgttcaag ctgcttttga taattctcga tccttctttg atccaaataa ttgctgagag
    70681 catctgctaa aaatggatat gtaaaaactg cagctccggc aagaaacatc actgccataa
    70741 ataattgtaa taaaagactg atttgggttt gaggtctatt acctattttc tttttcattt
    70801 tcttcacctt cttatttaaa aaaactgcag caacctttat aaaaccgatt gctgcagctt
    70861 atttttgttg cttatacttc agacttcatt ttagatttac ggtaccagct gtacgctcca
    70921 atcatcaaac ttattccgat tagtaagaat gccaagagcc cgttcccacc agtagcagga
    70981 agtgacccgc gtttcatatt cggtactctt tctggccctg gattttctac tcctacgatc
    71041 tgaatttctt cattatagct tccataagag atgataaatt cagtaaaggc tgcctcacct
    71101 gttggaagta cataaccttc tggcgccatg gtctctttca tttgataggt tccataatct
    71161 aacccttgaa cctcaaagta tccatttcca tctgcgacaa attctgttgc ttgtccgctt
    71221 gttgcccact caattctatc tgctcctgat tcataaacag ctaaaggcga ctccccttta
    71281 taaaatattg cgtattcaga acggtcttca ttccacaatt cgaatcttgc atctttaagc
    71341 aattcactgc ttgatgcatc atatttttca aactttcttc cacctgttgt gactttctct
    71401 ggcggctcta ctccttcttc ataatcaaat tcatcatttc gtccgatttc aataaccgct
    71461 gtgttgttat ggaaatcatc cgcttctagt tctgtggaaa ccttcaaatt atacgtgatc
    71521 gttaaatctc ctccacgtgc atctgctaat ctacctaaag tatctacttt ggctactcca
    71581 ttttcttctg tcaaaatcgt cacgacaaac ccgttgctta ctacttctac tatataatct
    71641 tgtccttttg ttaaaatcgt ctcgccggct cgcacttcta tttcctgatc ataataagtt
    71701 agaccaactg taggcgtatc tgtgattttg taatttttga taacagcgct tccgttttca
    71761 aggacagaat cgatgaaata aggaataggt gctgtgatcg tgtaagaagc cgtttttcct
    71821 ctttctagat caatataaga aatcgtttct tgttctccag cttcattaat aatcgttact
    71881 tgtctaaggt cgctttcctc tacattcatc tgtttatctg cttctctgat ttcgtttttc
    71941 ggatacaaat gcacatctgt attgtatgtg tctttccatg tagaaccatc ccacacgcca
    72001 tccaccatat caggcatcat cactggcatc ataacaacca ttggagacgc gattctggaa
    72061 atatgagctg gcgaataagt ttctaagaaa agatagactt tatctctaga agaaggtgct
    72121 tcttccgtca cttggacggt agagaatacc gcttctccag cttcattcgt tgtttgtttg
    72181 tcaatacttg tcagtcgata ttttaaaaac caactttctg tgtgccgttt ttgaatccat
    72241 tcaatcgctt cggcagaatt aagaccgtct tctcgttccg gtgttaaagg attatctcga
    72301 atcaaatcgt aatatacttc cgttacgtca aacaaatcaa aatcgactcc tggaatattt
    72361 tcttcaccaa aatccgtaga aaccaatccg ctattcggtc gttcttccgg aacagatgag
    72421 aaaccttttt tgtgtaatgt aatcgtcact tcacttggtt tttgtgcgac ttcttcggct
    72481 ttgactagtt ttccgttccc gaaaatccct attcccgcag taacgagtaa cagtacactc
    72541 attcccgtga caatcatttt tttattcatt tttttctccc cgttcttttt ttattaataa
    72601 aacagcacct aacaataaaa gaattcctag taaagaaatc aacgttcttg cttcattcgt
    72661 ttgcgggaat acggaccatt ttcctgattg acctggattg cttggaccac tcggctcttt
    72721 cggtttgtcc gttgtttctt ttacggtctc gttataaaca cgtggtgttg cggattcata
    72781 ggcagagact ggaactttcc cggattctaa ttcagccatc tcctgtccgt taactactac
    72841 atattgtggt tggccttttg catccaccca aaattcaggg atgatgacct cgattctctt
    72901 acttgcttca tccatctcgt aaccttccac accttttaat tcttcaaaat aataggtgcc
    72961 agaaggcaag aaacgttgtc ctgtcgtaac aaggccctct ttatccgaaa tgaatttgct
    73021 tacatttttg tcattcaaag gatcattgtc tgctggtttg atccattgat ttttcaagtc
    73081 attagcagga gccatatcta aataatattt ccccccttct atcatctgat atagagcaaa
    73141 aacagcacct tctaatggtt ttcctttttc agttgttcct ctttgacgtc catacttgaa
    73201 gaagtatgga tctcttagat aaccgacatt tttaggatag atatgaatct cttgcagttc
    73261 ctcggttgat tctgtcgggt gataaattgg caaaatagca gcaattggga tagctgtctg
    73321 atcgatatca acatccaatc caacttctgg atcaatcgat tcttcgaaaa tcaaataaac
    73381 tgcatcccga tcttggtttt tcttaggtgc tttgatacgt gcaatcccgt ctcctaatcc
    73441 gccaaccgta tctttcccag ttgttaccga agtccctatt tggacaagcc tttcttctgt
    73501 gattagctga cggatattgt ttctgttcgt ttcagcgatt tgctggacga attctttagg
    73561 agtcatgttt tcagcagctt ttttttcatg ataataatcg gtcatgtcgt aaatggcaaa
    73621 tgtcgctcca tttagtggca ccgtctgtga aatgatatct gctgtttccc ctgcttcctc
    73681 agagatagcc aagccatcat tttcatggta ttcaaattga tcgttataat cgatatctcg
    73741 aatcatccgt ttatgcaaca cgaattccac gacatcttcc cctgattctt ctgccgacat
    73801 cgcatgtgga agaaggactg ctgcaagcgg tagtaaaaca agtaattgaa gcatcaaaca
    73861 caacagcttt ttcattacct tgctccctcc tttttagatt gtctgacaaa caaaccatag
    73921 gctgctcctg caagcgccat gaagaatgca gcaagcagga tatagaataa tcgtccggat
    73981 cctcctgtag caggcaacgg attttcagga tcgttcccga cactaagctg taatctgatt
    74041 cgatttgtct ccaaaagttc atattctaac tcttgctgta gatcaggcgt ctctgcttct
    74101 tcgtttggat aatacgaaat ctcaactttt agtgtacccg cttcttttcc tggaactacc
    74161 cgatcatttt ctatgtcagg ttcttctcga taactaattt ctaaaataaa gtaacctgga
    74221 agttttctat agctgtcagg tgacttcact tccttgatcc gatagacccc ttccaacaag
    74281 tctgaagcat tgaataggga agaatcgttt ggatctggtg ccaagcctcc ctcgatgatt
    74341 tcatactctc ctgcactatt ttgtttctca attgaaaatt ctgcgccttc aagacgtcgg
    74401 ctattcatac tgtcaatttt caaaagttcc aaatcgattg gcgctagttc atcttctaag
    74461 gttacttgta gtacaccatc gatgatttca tagccttccg gcaagggagc agtttcgtct
    74521 tcgatgaccg tatcttcaat gataaactta ccttcttcat tcagtgcaaa ctggaacact
    74581 tgatcagata gccgataccc atttggtgcg acagattccg agacctcata ttcaccaata
    74641 gacagatgca gaggttggtc gtaatttaaa tcgacatact tttctactcc tacggcacga
    74701 gaatagaagt atccgatccc aggcaaacgt ttcgattctt ctggttctaa cggattacca
    74761 gtgtatgctc gatacagcgg gaaggaagaa tcccccactt tcctgatatc gaaacctgca
    74821 ccatctaatg tatggccttc atggtctaat tttctaactc ggatctcccg ataatcaaac
    74881 gtattgtaag cagtgaatgc tacccataaa cgatcctctt catctgttgt atacgtgaga
    74941 tattgccaat tttcttcacc tgtttgcttg agttggatag tccctatagc attatcgtaa
    75001 agtcgcaaga tccatgtgtc attttcatca ggttctttga aaccttctgg gttctggctc
    75061 tcaatgattt tgtattctgc atagttggta gtttcttcac tatccttata aacaaatgaa
    75121 tctggctctc cgattggatg aataactttc tcaccatcca cgattacata tttcccgtct
    75181 tcatctactg aatagaacgt gagtctccct ttctcttcct ctacttcgtt cacaccagaa
    75241 agccctttcg ctactggata tccgatttcc agtccgttac catctgtttc ataaagttca
    75301 aactctgcgc cattgattgg catattactg ccttcaatta gtttattgat attcatgtgg
    75361 attggcttga attcatttcg catttccagt ttgacataca actcatcata ctctgtgcct
    75421 tctatgttag gctcttgtac aacagaaagc ttgtgatatt ctccttcata ataggaattc
    75481 ggatctttac cttccggtcc gccgactctc gaaccagatg tatattcatc tacaagctcg
    75541 tagtgaccta acatctcatc ttcgttggct gcatattttt tgatttggaa ttgatacgtc
    75601 tcatctacta actgataacg tggtggtcca gcgatctctc tgatccaaaa tatgtcgctg
    75661 attgctccag tttcatctcc taaaatcggc atcttcgttt caaagatcgc tattccgtcc
    75721 gtggtagttt tacttgtttc gattggttcg gcttccccac tctctggatt ttggtcatat
    75781 aatgcaaatt cgactccagc caacggctcg ccattttgac ccttcttgct gacttggaaa
    75841 ttacggcctt tatgttcgta acgaatggaa gaagatggga aatacatccg tccagtagtg
    75901 atatcattct cataatcctc caggttctga atataggcca tattgttcac tgtgcaaaaa
    75961 tttccgtctt gagctcccgc atcaagtttt acttggtact tgatcctgac catttcccct
    76021 tcacctaaag aaatcggaga aaatttataa gcttggtttt cccgtccgac atacgttgtc
    76081 ggagtcgcca ctacttctgg tggggcttgt ccatgacgtt cttcatccca tatttctggc
    76141 tcttggtact tcatgccgtt ttctcctgga tctagatggt aaaactgaat atctgccgat
    76201 ctgccagctc caaatggtga gacattaaaa ccagtttcat cataaagtgt ggcttgctgg
    76261 atcgttctct tgaaggtaga agagatatcc tccaaggctg taacaatgtc attcttgttg
    76321 ttcgcattgt aatatcgcgg tgttacgcca tcttcttcta aatcagcaat atttttcaat
    76381 acatttcgtc caatagcatc tagtcgttgt ctcccgctaa cgtttccatc gattcctaaa
    76441 ccaatggaga agatacttgg cgggttctct tgatacttgt ttttcagcca atgatgataa
    76501 gcaactgtgt tgatcgcaga tgacttttct tgcgaagtag gactgccatt cgtattgtta
    76561 ttagaagaac catcaccaac ccagtgagtc gctttattat ctccttcttt tacctctcca
    76621 taacgatagt gtacatttac ttcatcttct ggacggttaa agtcagatgc ataggtaact
    76681 ggatgagtaa accctgcatt agaagtattc ccagaaaact cctcaaaatt tctgaacaaa
    76741 acgtcattat cttcagcaat cttatcttcc atcacagtcc agttgctcca agctccatta
    76801 gtgctcgtac gataacttga ctgaatcggt gcataactga aggtcggtgt cccatctccc
    76861 acaacgatta ggattttttc tggatttcta ttttctccgc cattgtcttt atacaatgtt
    76921 tcatagccat ttttaagccc tagtgtaagc ggagttcctc cgattggtgt acgatttaat
    76981 gctgtattct cacttcccgt caaacgattg atatcatttg tcaatggaaa gatgtccgaa
    77041 ctgatttgct catggttatt gatagctgta ctattatggt aaaaattcac cataccgatt
    77101 cgaatatcca gactcgggtc agagagcaga tctttactca tttcgtttac tgcctcaatc
    77161 aacgcagcat ttttagtttg actattggtc ccagcggtta attcactcat actcgctgac
    77221 ttatctagaa caaaaacgat atcgattgga ctgatttcct ttttctcgcc cccgatgaca
    77281 tctagataga cattgtaaag ggttgttgca tcttcaggat gtagatcaga tgctgtgctg
    77341 cttggcagaa caggttctac ccattttttg atgtacgccc cgttataatc caagtaactg
    77401 ttttcaaaat tccgttcatt tccccaaagc tgaacggttt gagctggaac gtttctatct
    77461 tcttcgcctg cagttgatcc attattagta gcatagtcgt aatttcgcca gttcgctccc
    77521 ccgtttgtat aatcactatc atcgatcaag ttctctccgt catatggcga gcctttgtct
    77581 ccttgatagc gttcattgct ttgtgtccaa tttcctaaca cttttgtata gtcattcgtt
    77641 gtaaacttca tctattcttt catcaaccac cacttgaaga ggtagttacc cttcgttcac
    77701 atcggggatc ttttctgttt caaaaatcaa tgtgccggtt tctgcttctt ttgaataaac
    77761 ttttccgaca taccattctt gaccatcgat cgtctcgact tttaggtctt ctccatcttc
    77821 atcataggag gatagacctg ttccacgaac gttttttacg gtaccaatcc cggcggctgc
    77881 cgcatcgatt gctaaacgta ttctaccttc attggcactt tccttttttt caaaagccag
    77941 atgccattca agggtttctt ctttttcaat atacttccct tcaatttttg catagtcctc
    78001 atctaagaat acctcatcct cctttgtttc tgttgactca gcagctacat ccagggtcgt
    78061 catatgatag actgactgga aatatggtcc aaatagcaaa ctgacagtta ctaactgcaa
    78121 aagcttcaat tttaaccctt tcacatcctt atactccttt caaaaatatt tgtcttcttt
    78181 cacaacaatc aacaaaacaa tgcccatcta attataattt aatttaattt taattttaaa
    78241 tcaaatgata tcctttttag ttaaaaaaca ccaattatac aacatttatt attaaaatta
    78301 aattataaaa ttttataacg ttctcattta acaaaactta agttattaag acaaatgaat
    78361 aaacaactac gctttcctcg aaacaaaaaa ataaagcaca aatgcgcatt tacgcactca
    78421 tgctttattc gtacaattaa ttcagataac ggcagcgcat aggatgccaa taacacttcc
    78481 taaaatagct cctcctaaaa tgtcagtagg ataatgtaaa agtaaaaaaa tcctcgaata
    78541 agcgatcaaa caagcaaata aaaaggctaa tattcctgca actggactta attcgaacaa
    78601 cgcagctact gataaaaagg ctagcgctgt atgtcctgaa ggaaaagaag aagtcttcgg
    78661 tttacttgtg taaaggacaa gccgacgatt tttttcataa ggtcttttcc gtcttgtcgt
    78721 ttttttgata aaaagcaaac caacaaaaaa atagatcaaa ctgccgataa gaatggctag
    78781 accatattct ctagtatcag gtatcaagac taaaacagca gcaaggagca accaaataaa
    78841 accaccatta gagatattac tgataaattt caaaaaattg atttttcgac tatccgtttt
    78901 ccgttgcttg gcgaacttgt tgataatact atcatcgatt ttagcaatca aacgcaacat
    78961 gttattcctc ctttttaaga accttttctt gcgtacgatg gataaaaagc aggacttgaa
    79021 aataaaacaa gaaactgaaa cctaataaag caaaatagat tggtcctgca aaccagaatg
    79081 cgacaagaag gatcaacgtg cctgccaaat agaaaagcgc gcttttgaaa tcattaaaaa
    79141 acaagaaaaa tgcagtaatc caagaagctt ctttttgttc cagttgaagc aagattttgt
    79201 aactaaagag aagcgcgctt aatagaagca aaaacaacgt agtatacatc aatgtatagc
    79261 caattccccc accccaatag cggaaaatct gatagtcgat caatagaaga agaaaataaa
    79321 cacttgctaa actatcttgc tttccataaa gagagaagtt ctcacgaaac tttttccaga
    79381 atagtgaacc gagctttttc tcttcatttt ttttgtaatg ataaatcata tacagaactg
    79441 ttcgacttgc agttcctaat gtgaaaacag gcaagacgca caggaaccat agtagctgta
    79501 aatggataag acgtggaatc cattcaatga tttctaagaa ataatcattg gcttttttac
    79561 caatcgatcg attcatcatc cttttgaacc tcctgtcaaa tccatccctt caatgaaata
    79621 acgttgtgca aaaaagaaca gcaaaatcgt cgggatcaag acaagtgttg ctcctgccat
    79681 cagataattc cattgtgtcg ttgcttggtt ctgaaaaact tgcaagccaa tctgtaacgt
    79741 gtatttttct tgatcttgga tatataacag aggacctaag aaatcattcc aagctgcgtt
    79801 gaaggaattg atcgcaatgg taatcaaggc aggtttcgtc aatggaagca tcaaatgact
    79861 ccaaatatac aaatgattcg ctccgtcgat ttcagcagct tcgatcaatt cattattgat
    79921 cgataagtaa aattgacgca tcagaaatat attgaacgca ttgccgaaga acgatggaac
    79981 gatcaggggc aaataagtac ctacccagcc gatcttggag aataatacat attgagggat
    80041 catcgttaca aaccccggga tcatcatcgt agaaagaacc aaagcaaaca tcagcttttt
    80101 cccaggaaaa tcaagcttcg caaatccata agcaataaaa gaatttgaaa ggacattacc
    80161 gatcactact agcacagtga tgaataatgt atttcttgca tagcgccaaa aaggaaaggc
    80221 ggcaatcgta tccagatagt tttcccatac gattttttct gggaaaaagg tcggaggata
    80281 acgaacgatt tcttccattg gtttcaatga agtaaacacc atccaaagca atagaatgat
    80341 caaaatgatt cctattactg tcatgaggac ataacttagt attttttttg tttgccgatt
    80401 cacactggtc tttttgaaat aatccatcat ttgccgtcac ctccagaata atagacccac
    80461 ttaggtgcaa gcttcaaatt gacaaacgtc agaaccaaca cgatcagaaa caggatcatt
    80521 gacatagcca tcgcataacc catatcaaaa gctacgaacg ccttgttcca catatgcaga
    80581 ttataaaaaa gcaaagaatt tgcaggtccg cctgttccat ttttcgtcat gacataagct
    80641 tcttgaaaaa tttggaaaga accgatcgta gaggtgatca catcgtaaaa aataatagga
    80701 gtgattaatg gcaaagtaat acttttgaac tttccccaaa cgcctgctcc atcgatttct
    80761 gctgattcat ataaagatct aggaacattt tgtaatgtag ctaagtaaag tagcattgct
    80821 cctccagaac tccatatttt catcaaaatc aacgcaggtt tcgtccattg tggatcaaac
    80881 agccaagctg gtccagggat atggaaccag cccagaaatg tgttgatcaa tcctgcagat
    80941 ggagaaagca gctgcatcca taagatgtag actgctactc ctgataagac tgccggtaaa
    81001 tagaaagctg tacggaatac actgattcct ttgatttttt gattgagtac tgtggctaaa
    81061 aacacactga aaatcgtcgt cagcggtaca ttgaagatga cgaaataaac ggtgttgtat
    81121 aacgatttcc aaaataaatc atcgtgaacg aacattcttt tgaaatttgc tagaccaatg
    81181 aaattcatct tggatgtcaa attataatcg gtgaaactac caataactga aaaaagcata
    81241 ggccctagca taaataccaa gaacccgata ataaaaggcg agataaataa ataggcccat
    81301 cttgcttctt ttttctttcg gatagataaa cgcttcaaat tttcttgcct cctctgtacc
    81361 tagacagtca aaaatggatt attgattttg cttgactaag ttttctactg ctttttgtgc
    81421 atctgccaat ccttcttttg gtgattttgt tcctaaaaga atctggtcaa tctgttcatt
    81481 cactaatgtc gtgtaatccg gagcatctaa aggaactggt gtgatcaccg tatttttaaa
    81541 gttttcgtcc gccatttgat agatcatttt tcctttatcc gttaggttgt cattattcac
    81601 taaattctca ttggctgtct tgctcgccat gatatcaaaa cttttttcgc cgaatttttc
    81661 ttgtacttct ggagaagaaa gatatttgat gaattcataa gaggcctcag gatcttttgc
    81721 tccgtatggt acttccaaga cgaatccgcc accccacgac caatggcctg aaccttcttc
    81781 tttttctggg agctgcatca cgccaaaatc aaaatcatcc ggtgcatttt ccatcaaact
    81841 agagtaataa ttgatatttt gggcacgcat ggctactaat ccggaaataa atggatcaga
    81901 tacaccagac ccaaattcgg cttctaattt attgatcgta tttttcccat aataatcttg
    81961 ccagtcttgg atccactcca aggcttccac tttattttta gtatcaatct tgactttttc
    82021 atctttgtca aaccaactag tcccgttatc tgcgtttagc gcccatacat cagcccctaa
    82081 attccataat ggataaaaac cgatgcgtgt aaattctttt ccttcttttt tatctagttt
    82141 acgtgctgtt tcttctaatt catcccaagt tgtcggtaga tcttgttcac tgattccagc
    82201 ttctttaaat aactttttat tataaaaaat gacttgtgta tctgtattga atgggatcgc
    82261 ataaggatca ccttcatatt ctactgtatc ccaaagctgc ggataaaaat ccttagaagt
    82321 gtctttttct acatatttgg agagattagt tgcttgctgt gcttctgctc tttgggcaac
    82381 ggaatttatg tcctgtacaa taacatcggg tgcatttcca gctgttattg cagataaaga
    82441 tttagtccag atgtcacccc atggctgata cttataattt acatggatct cgttttgtga
    82501 ttgattgaaa tcctcaatga tttcttcgat agcttctttt cgtgcaccgg atccccaaaa
    82561 ggaccagaaa tcaatatttt ttcttccatc acttgttttt tcagaagaat ttgctgcact
    82621 tgaactggca tcgcttgcat tacttgatcc gcatcctcct aataaaaaca atgctgaact
    82681 tgccagcaac cctgtaaaca ataatttttt catattttac ctccaaattt taatgtaacc
    82741 gatgccggtt ttaatataac gatatcattt tgttctgaaa ataacaattt ataaggtatg
    82801 attctaaaaa aattattaat gataccaata ctttatataa ttcgcgagcg ctttctttga
    82861 attttttaat ctttcgtagc agaatgagaa tgtattaaaa atagattagg aggaattttg
    82921 atgtttagac accagaaaga attgcagttt gaagcaaaac cagatcgtcc gaatccatta
    82981 atcgcgaaat atctgcagga gctgatcggc ggccaatatg gcgagatgag tgtagctatg
    83041 cagtacctgt tccaaggctg gagctgccga ggagaagaaa aatataaaga tatgctgatg
    83101 gacattgcaa cagaagaaat cggccacgtg gaaatgttgg cgacaatggt tgctcgttta
    83161 ttagagggtg cgcctgttga agatcaagaa gcagcgatgc aaaaagatcc aacaatcggt
    83221 gccattatct ctggaatgaa tccacagcac gcgatcgtag ctggcttagg acctcgtcct
    83281 gctgacagcg tgggcaatcc atggagcggt gcctatatca ttgcgagtgg caacttgtta
    83341 gcagacttta gagctaattt aaatgccgaa tcacaaggaa gacttcaagt agcacgtatt
    83401 tacgaaatga tcgatgatcg tggcgtaaaa gatatgctgt ctgttctcct tgcaagagat
    83461 agctatcatc aaaatctatg ggaatgtgca gtgaaagaat tagaagaaaa agaaggttca
    83521 atcttagtcc caagtacttt cccgcgtgac aaagaacgaa aagacattgc ttatgacttc
    83581 tataacttct cagagggaga ccaaagtagt gaaggttctt gggctagtgg caaagcactg
    83641 gatggcgaag gcgaatatca atggttgaaa gaacctaaga tcgaaggcgc tgcaccaaaa
    83701 ttagaaccag taacaccaaa aatgtatggg acacctccat tcaagaaata aatatctacc
    83761 cgtagaaaca cccgtttttt gttccacacg ctttgtgagc aaaactgact aaagtcatta
    83821 atcataacca cccttcaaaa gggtggtttt ctctagagct ataagctcaa atcaccggcc
    83881 agcgcctaaa gacgctggct ttcatatttt atgttcaagc catattgcct ttgccagttt
    83941 tgcccacctc ttaaaaggtt tatgtactac tccttagccc ttaaaaggat cgtcttactc
    84001 tcgtacactc agcttatcta acgcactatc atgtttttct tgttcttgaa tgtacttttt
    84061 gatggtcgct tcattttgtc ctacttttgt cacatagtat ccttccaccc aaaagtgtcg
    84121 atacccaaat ttatatttta gatttgcaca atctcttgat gatctcctga atattggctc
    84181 gatattgatt ataaattatt tttcttctat actttggtgt gaatacaatg tggtatttac
    84241 acatctactt tgtacgggct aaactatttc cctttattgc tccacccgca tagcaagtgg
    84301 ttttgtattt caatcgctcg gcgcgctcaa ttaggtctaa agacaataaa tctaaaaacc
    84361 cgctgcttca ctgacagaaa caacgggttt tcagaaaaaa atgaaatgaa aaagatctct
    84421 tacggcaatc tattttattt ttttgatttt ttataaatgg aaaatgcttc aagaagtcca
    84481 agaaataaag ccgtataaaa acaagaacct atgaaatctt tatttattag atattcaaca
    84541 gaaatcccga taacactagc caacaatata tttatcagaa gtgtaaaata aggatgcttt
    84601 ttatagaata gaactagtgt tgttttcata gactctcctt tcttcatatt tccaattaat
    84661 cacaaattca cggcattact agaaaaatta aaggggcgca gaagtggaaa aatactcttc
    84721 gactttccct acaaaccagg ccatcttgtg tttaataact ataccaaatc taaggtacct
    84781 ctaaatctaa attccatttg tggcggtgac ctccaatttt taactcttct atctcccatc
    84841 cagtatacat aaccttgata cttcgaccac ttatatatcg aacacacaaa tacctatcca
    84901 ttgcaggagt actacctaac ttagttgcta cttgcatcta aataacagct gaaaagatag
    84961 gacttactct tagtaaagta tctgaactat taatttttaa actatcattt ttatcctcaa
    85021 ctacatgcac atctgttgca acttcacttt ctgtctcatc cagataaatg attaaggaat
    85081 cgggaattgc aatatcttca gctgacgctg gaatagttga gctaaattag tcatcatatc
    85141 tttattataa aataaccaac agcaaaaaat acaatgaaaa acaaacttgg aactaatgta
    85201 tcattaataa atgttgaaaa attcagcaac tcattactgt atgattttag cagcccaacc
    85261 aacatatacc ctataaaagc taataagatg gttaggaatc ctttagaatt tt
//
LOCUS       DAFEPQ010000005        84974 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.005, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000005
VERSION     DAFEPQ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 84974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.005"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(552..2165)
                     /locus_tag="LP543_000352"
     CDS             complement(552..2165)
                     /locus_tag="LP543_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL2320547.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..3214)
                     /locus_tag="LP543_000353"
     CDS             complement(2306..3214)
                     /locus_tag="LP543_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2320548.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3397..4521
                     /locus_tag="LP543_000354"
     CDS             3397..4521
                     /locus_tag="LP543_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL2320549.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4748..5665
                     /locus_tag="LP543_000355"
     CDS             4748..5665
                     /locus_tag="LP543_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2320550.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5658..6491
                     /locus_tag="LP543_000356"
     CDS             5658..6491
                     /locus_tag="LP543_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2320551.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6574..7692
                     /locus_tag="LP543_000357"
     CDS             6574..7692
                     /locus_tag="LP543_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL2320552.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7686..9584
                     /locus_tag="LP543_000358"
     CDS             7686..9584
                     /locus_tag="LP543_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBL2320553.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9578..10801
                     /locus_tag="LP543_000359"
     CDS             9578..10801
                     /locus_tag="LP543_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL2320554.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10887..12821)
                     /locus_tag="LP543_000360"
     CDS             complement(10887..12821)
                     /locus_tag="LP543_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL2320555.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13047..13703
                     /locus_tag="LP543_000361"
     CDS             13047..13703
                     /locus_tag="LP543_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBL2320556.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13777..14130)
                     /locus_tag="LP543_000362"
     CDS             complement(13777..14130)
                     /locus_tag="LP543_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBL2320557.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14322..15251
                     /locus_tag="LP543_000363"
     CDS             14322..15251
                     /locus_tag="LP543_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBL2320558.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15262..16098
                     /locus_tag="LP543_000364"
     CDS             15262..16098
                     /locus_tag="LP543_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBL2320559.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16346..17122
                     /locus_tag="LP543_000365"
     CDS             16346..17122
                     /locus_tag="LP543_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2320560.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17298..18041
                     /locus_tag="LP543_000366"
     CDS             17298..18041
                     /locus_tag="LP543_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2320561.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18034..19614
                     /locus_tag="LP543_000367"
     CDS             18034..19614
                     /locus_tag="LP543_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBL2320562.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19865..20344)
                     /locus_tag="LP543_000368"
     CDS             complement(19865..20344)
                     /locus_tag="LP543_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBL2320563.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20360..21112)
                     /locus_tag="LP543_000369"
     CDS             complement(20360..21112)
                     /locus_tag="LP543_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBL2320564.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21229..21299
                     /locus_tag="LP543_000370"
     tRNA            21229..21299
                     /locus_tag="LP543_000370"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21261..21263,aa:Gly,seq:ccc)
     gene            21660..22385
                     /locus_tag="LP543_000371"
     CDS             21660..22385
                     /locus_tag="LP543_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBL2320565.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22640..22906
                     /locus_tag="LP543_000372"
     CDS             22640..22906
                     /locus_tag="LP543_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBL2320566.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22903..23334
                     /gene="ruvX"
                     /locus_tag="LP543_000373"
     CDS             22903..23334
                     /gene="ruvX"
                     /locus_tag="LP543_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBL2320567.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23359..23664
                     /locus_tag="LP543_000374"
     CDS             23359..23664
                     /locus_tag="LP543_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBL2320568.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            24233..25069
                     /locus_tag="LP543_000375"
     CDS             24233..25069
                     /locus_tag="LP543_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBL2320569.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            25134..25565
                     /locus_tag="LP543_000376"
     CDS             25134..25565
                     /locus_tag="LP543_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2320570.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEA
                     KKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(25633..26955)
                     /locus_tag="LP543_000377"
     CDS             complement(25633..26955)
                     /locus_tag="LP543_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBL2320571.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(27109..28749)
                     /locus_tag="LP543_000378"
     CDS             complement(27109..28749)
                     /locus_tag="LP543_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBL2320572.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            29079..29549
                     /locus_tag="LP543_000379"
     CDS             29079..29549
                     /locus_tag="LP543_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBL2320573.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            30178..31281
                     /locus_tag="LP543_000380"
     CDS             30178..31281
                     /locus_tag="LP543_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBL2320574.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            31565..32245
                     /locus_tag="LP543_000381"
     CDS             31565..32245
                     /locus_tag="LP543_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBL2320575.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            32335..33369
                     /locus_tag="LP543_000382"
     CDS             32335..33369
                     /locus_tag="LP543_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBL2320576.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            33383..33718
                     /locus_tag="LP543_000383"
     CDS             33383..33718
                     /locus_tag="LP543_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320577.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            33702..36182
                     /locus_tag="LP543_000384"
     CDS             33702..36182
                     /locus_tag="LP543_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320578.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            36238..37797
                     /locus_tag="LP543_000385"
     CDS             36238..37797
                     /locus_tag="LP543_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2320579.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            37794..39158
                     /locus_tag="LP543_000386"
     CDS             37794..39158
                     /locus_tag="LP543_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL2320580.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            39329..40399
                     /locus_tag="LP543_000387"
     CDS             39329..40399
                     /locus_tag="LP543_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2320581.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            40414..41082
                     /locus_tag="LP543_000388"
     CDS             40414..41082
                     /locus_tag="LP543_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2320582.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            41102..41680
                     /locus_tag="LP543_000389"
     CDS             41102..41680
                     /locus_tag="LP543_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL2320583.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            42083..42994
                     /locus_tag="LP543_000390"
     CDS             42083..42994
                     /locus_tag="LP543_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL2320584.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(43250..44167)
                     /locus_tag="LP543_000391"
     CDS             complement(43250..44167)
                     /locus_tag="LP543_000391"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBL2320585.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            44369..44806
                     /gene="zapA"
                     /locus_tag="LP543_000392"
     CDS             44369..44806
                     /gene="zapA"
                     /locus_tag="LP543_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBL2320586.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            44864..45412
                     /locus_tag="LP543_000393"
     CDS             44864..45412
                     /locus_tag="LP543_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HBL2320587.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            45527..47887
                     /locus_tag="LP543_000394"
     CDS             45527..47887
                     /locus_tag="LP543_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBL2320588.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            47974..48288
                     /gene="trxA"
                     /locus_tag="LP543_000395"
     CDS             47974..48288
                     /gene="trxA"
                     /locus_tag="LP543_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBL2320589.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            48470..50248
                     /gene="uvrC"
                     /locus_tag="LP543_000396"
     CDS             48470..50248
                     /gene="uvrC"
                     /locus_tag="LP543_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBL2320590.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            50393..51292
                     /locus_tag="LP543_000397"
     CDS             50393..51292
                     /locus_tag="LP543_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2320591.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            51285..52547
                     /locus_tag="LP543_000398"
     CDS             51285..52547
                     /locus_tag="LP543_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2320592.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            52710..54608
                     /locus_tag="LP543_000399"
     CDS             52710..54608
                     /locus_tag="LP543_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBL2320593.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(54907..55626)
                     /locus_tag="LP543_000400"
     CDS             complement(54907..55626)
                     /locus_tag="LP543_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBL2320594.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            55963..56295
                     /locus_tag="LP543_000401"
     CDS             55963..56295
                     /locus_tag="LP543_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320595.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            56282..58285
                     /locus_tag="LP543_000402"
     CDS             56282..58285
                     /locus_tag="LP543_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBL2320596.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKHQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            58304..58774
                     /locus_tag="LP543_000403"
     CDS             58304..58774
                     /locus_tag="LP543_000403"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBL2320597.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            58795..59379
                     /locus_tag="LP543_000404"
     CDS             58795..59379
                     /locus_tag="LP543_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBL2320598.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            59394..60380
                     /locus_tag="LP543_000405"
     CDS             59394..60380
                     /locus_tag="LP543_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBL2320599.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            60370..60681
                     /locus_tag="LP543_000406"
     CDS             60370..60681
                     /locus_tag="LP543_000406"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBL2320600.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            60703..62484
                     /locus_tag="LP543_000407"
     CDS             60703..62484
                     /locus_tag="LP543_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBL2320601.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            62477..63853
                     /locus_tag="LP543_000408"
     CDS             62477..63853
                     /locus_tag="LP543_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBL2320602.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            63860..64495
                     /locus_tag="LP543_000409"
     CDS             63860..64495
                     /locus_tag="LP543_000409"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBL2320603.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            64525..65880
                     /locus_tag="LP543_000410"
     CDS             64525..65880
                     /locus_tag="LP543_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBL2320604.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(66072..67466)
                     /locus_tag="LP543_000411"
     CDS             complement(66072..67466)
                     /locus_tag="LP543_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBL2320605.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFNSPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            67558..68529
                     /locus_tag="LP543_000412"
     CDS             67558..68529
                     /locus_tag="LP543_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBL2320606.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            68526..69308
                     /locus_tag="LP543_000413"
     CDS             68526..69308
                     /locus_tag="LP543_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBL2320607.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(69477..69848)
                     /locus_tag="LP543_000414"
     CDS             complement(69477..69848)
                     /locus_tag="LP543_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL2320608.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            69982..70425
                     /locus_tag="LP543_000415"
     CDS             69982..70425
                     /locus_tag="LP543_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBL2320609.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(70468..71277)
                     /gene="yidA"
                     /locus_tag="LP543_000416"
     CDS             complement(70468..71277)
                     /gene="yidA"
                     /locus_tag="LP543_000416"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBL2320610.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(71296..72663)
                     /locus_tag="LP543_000417"
     CDS             complement(71296..72663)
                     /locus_tag="LP543_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL2320611.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(72647..73477)
                     /locus_tag="LP543_000418"
     CDS             complement(72647..73477)
                     /locus_tag="LP543_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBL2320612.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            73640..74077
                     /locus_tag="LP543_000419"
     CDS             73640..74077
                     /locus_tag="LP543_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBL2320613.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            74153..74773
                     /locus_tag="LP543_000420"
     CDS             74153..74773
                     /locus_tag="LP543_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBL2320614.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            74962..75468
                     /locus_tag="LP543_000421"
     CDS             74962..75468
                     /locus_tag="LP543_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBL2320615.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            75485..76384
                     /locus_tag="LP543_000422"
     CDS             75485..76384
                     /locus_tag="LP543_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBL2320616.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            76471..77355
                     /locus_tag="LP543_000423"
     CDS             76471..77355
                     /locus_tag="LP543_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBL2320617.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(77547..77618)
                     /locus_tag="LP543_000424"
     tRNA            complement(77547..77618)
                     /locus_tag="LP543_000424"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(77583..77585),aa:Arg,seq:ccg)
     gene            77678..79012
                     /gene="rpoN"
                     /locus_tag="LP543_000425"
     CDS             77678..79012
                     /gene="rpoN"
                     /locus_tag="LP543_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBL2320618.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            79161..80252
                     /locus_tag="LP543_000426"
     CDS             79161..80252
                     /locus_tag="LP543_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HBL2320619.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            80293..81294
                     /gene="gap"
                     /locus_tag="LP543_000427"
     CDS             80293..81294
                     /gene="gap"
                     /locus_tag="LP543_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBL2320620.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            81404..82594
                     /locus_tag="LP543_000428"
     CDS             81404..82594
                     /locus_tag="LP543_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBL2320621.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            82698..83453
                     /locus_tag="LP543_000429"
     CDS             82698..83453
                     /locus_tag="LP543_000429"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBL2320622.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            83569..84867
                     /gene="eno"
                     /locus_tag="LP543_000430"
     CDS             83569..84867
                     /gene="eno"
                     /locus_tag="LP543_000430"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBL2320623.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        27965 a        14690 c        17517 g        24802 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
     2941 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtagaaa
     3001 agatttggac ttcttgctgt ttatcaatga ataaaaggga aggattatct agttctatag
     3061 atttatgtgc aaattcagca tataaattag cgctttcatt tattagtagt cgaaaatagg
     3121 gagcagcaga taaatgaaga tgggtatcta tggaataggt tagtgttttt ttgacaagat
     3181 tggaggattg gttgtttgtt aacaaaagaa ccactttcct ttctcttttt atataaaaag
     3241 ctttctattt ataattaatg actattataa caaaaatgaa aagccaaaac gacaagtaaa
     3301 atattttttt atataaatat ttgtgttact aattccgtta atatacataa gtgaaagcgt
     3361 tttatataag aacttgaaaa gaaagaaggg atagacatga aaaaaagata caaattggtg
     3421 atagctttgc taaacattac cctattattc agcttaagtg catgtggaaa aactaacgaa
     3481 gaagtaacag caaacatcgc agatccaatg ataaaaaaag ccactacatt agaagtatat
     3541 gtcgcaaacg gcaaactgac aaactatgtt gaaaatgctg caaaaatcta taaccgcaaa
     3601 actggagcaa atattgactt aaaaattgtg aatgtagctt cgggaacagc aacagtacaa
     3661 atgatcaccc caaaattggt aacacacgaa gatatgccgg atattatctc catggcagac
     3721 acttcagcag caggtgtgct ggaaaaattt caagatgctt tttatagtgc tgatgaatat
     3781 ggattttatg acaaatatgg cgatcagttt tatgatcaaa aattgaatat tttgcgtcaa
     3841 caaacgagta atgaaacgat tattccttgg gctagtgatt ttaccccagc actttgttat
     3901 tatcaaccaa aaaaatttgc tgaaataggt gtagattttg aagaaatcag ttcttgggaa
     3961 gaatatatcc aggtggcaaa agcattaaaa gaaaaaactg gtagttttgg aattgccttg
     4021 ccagaatctg gggaccaaga attgtttatc aacatgctag cccagcagaa tcaatctttg
     4081 cttgatgaag aaggaaatat ccgtctgaat actaaagaag cgaaacatgc tgcaaagtta
     4141 atcaaagaaa tgatcgactc agatattgta catttctacg gtgcacaaga tgctgagaaa
     4201 gcatttcaag aatctgcaat gtttgttgct ggcggctggt atgctacgaa tatgtctttg
     4261 aattttcctg atgcatctga caaatggcgc atggctccac tgattccttt ttcaaaagaa
     4321 aatccaggaa aaggtgcagt atcaggagga tcaagttttt atgtccctaa agatgccaaa
     4381 aatccacaag tggcacaaca atttcttaca tttatgctga cagatgaaga atgcttagcc
     4441 aacgcactgg agctgggcgt ggctacatct aactcattgg cctatcaaac agaagcagct
     4501 gagaaaaagt ttgactacta aggaggacaa aaatactatg aactattgaa taaaatcaat
     4561 gaaaatacat caaatactgt tttaccagcc acttattcag atgctacgtc ttatttgact
     4621 accgctgcat ataactattg gcaaacaggt gattttgaaa attcatatct aaaagaagca
     4681 gaaaattttg ctcataagta cggcgtaaaa ttaactggtg attaaaaaat attaggagga
     4741 cttaacgatg atgcaatctt cgttaccaag caagacaata aagaacagca agaaaaaagc
     4801 aacagatacg tctcataaaa aaagtgctta tggattggtc acaccttttg ttattttctt
     4861 tattgtgacg attttgattc ccatcattat ctcgtttgct tttagtttta tgaaaattgg
     4921 ctactcattt gaatttgttg gatttgacaa ttatctagct atcttccgag atccattgtt
     4981 tattaaatct tacacgaatg ttttgattat catggcagga tcaattccaa taacgatgct
     5041 attagctatt tactttgctg ttttattgaa ttcagcacag ttaaaaggaa aaggattttt
     5101 tagaactgtc tattatattc caaccattac atcaacggtg gctgtagctt ccgtattcat
     5161 gacttttttc aatccgactg ggttattcaa tggatttttg aatgcgctag gaattaacgg
     5221 tgtacagtgg ttaacaaatc ctttttggat acgaatatca atgatcatca cgatgatttg
     5281 gcttagtgta ggatataaca ctattttatt tttagctggt cttcaaggga tttctaaaga
     5341 gatatacgaa agtgctgaaa tagatggagc atcgaaattt cggcaattct ttttattaac
     5401 gatccccatg ctaaagccaa tcattttaat ggccacagtt ttagccacta tcaatggctt
     5461 gggtaatttc aatatcccaa atatattctt tgggacttcc aatggaccag aaaattcagc
     5521 tttggtcgta ggagtcaatt tatataaaac aagttttgaa atggttgatt ttggaaaagc
     5581 ctctgctatt gcctggacga tggtgtttgt atctgttata ttatcggcta ttcaatttaa
     5641 attcggaggt gataaaaatg actaaacaaa agatacaaag cagaattttt acgtacgtca
     5701 ttattgtgtt tggtgcctta atatttttga tgccatattt atatatgatt gtagcttcaa
     5761 cgcaaagtaa tcaagaaatt ttaagtggcg gaatcaattt caagtttggg gatatgctat
     5821 taaaaaactg gcaagccatc atggcgagat ataattatcc cttgattatt tggaattcga
     5881 ttgttattgc tgtaatcagt acagttttag ctacagccag ttctacaatg gcgggttatg
     5941 cattagcaaa gtatcgtttt atggggaata aactgttgtt caatacagtt atgctatcta
     6001 ggatggtgcc tttttttacc acattgattc ctttatttta cattatgagt cgtttaggat
     6061 tatcgaatac ttatgcaggt gtgatcatcc cttcgattgc cagtacaact tcagtgttta
     6121 tgatgcgaca atatagcagt caatttccta cagcactgat ggaggctgcc agaatcgatg
     6181 gtgccaatga gtgggtgatt tttacgaaaa tcgctgtacc gattttatca cctacgatta
     6241 ttactagtgg tttacttatc tttatgggaa gttggaatca atatctattt ccattagtca
     6301 tgctttcaga taaagataaa tatacggttc ccttaattat caaaaatatg acggtttcta
     6361 gtacaggtga gccaattaat tatggcgcaa ttatgctaat tttagcaact tcagtgatcc
     6421 caatggtctt gatttatgtg tgggcgcagt ctaaatttaa ggagaatgac attggagctg
     6481 caaataagta aaaaaaataa cgaatgttaa ccatctagtt cgttctagga gcaactccac
     6541 attcataaaa attaaaagaa tggcgggata tccatgaatg aacagacgaa ggaatggtca
     6601 agaaaaattt tgaatgtagc ttctgaaaag atagatgcgg aattagacag aatggggaat
     6661 aaaattccat atatttctta caacggaaaa tatacagacg taataaagga cagaggaatc
     6721 gactggtgga ccaatggttt ttggagtggt attttatggc agctctttca caagacaaaa
     6781 cgagaaaagt tcaaagaggc agcaatacaa caagaacaat tattagaatc tgccctgtac
     6841 catttttcag acgttcatca tgatgtaggt tttttattct taccatcggc tattgcacat
     6901 tatcaacagt ctcaaaacaa acatagtcga caactagggt tacatgcagc tacggtactt
     6961 gctggacgct ataatatttc agggaactat ttagtagcat ggaatgacca taaacctggt
     7021 tggatgatta ttgatagtat gatgaattta ccattactgt attgggcttc gagggaaact
     7081 ggtgatccga ggttctctaa aattgctcgg cagcatgcag atacaacagc taagtatcta
     7141 gtgaggaaag atggatcaac cgggcatatc gcagaatttg atgcagaatc agggaaatat
     7201 attcggttac ttggcgggca aggatacaat gagaattctt gttggagcag aggaaatgca
     7261 tgggctttat atggattcgc gttaagttat cgttacacaa aaaatccgta ctatctagct
     7321 atagcagaaa agatagcagt aaattttatt cagaaaatcg aaaaaactgg ttatattcct
     7381 ctcattgatt ttgctgtaaa agattcgaat attgatacca gtgctggagt atgcgctgca
     7441 tgcggtcttc tggaattagc aggatatgtt tcctctgtac gatcaaaaat gtatcatgaa
     7501 gctgctatca atatactcca aaagacagaa aagcactatg caaattggaa tatggatgaa
     7561 gatgggatca ttggaggcgg agcagaagca taccatagag aactgactta taatgtgcca
     7621 ctgatctatt ctgattattt ttttattgaa ggcttacttc gattagaaca gtcggaatta
     7681 gctatttggt agtaaaaaga ggagaaaata caatgaaaca tccagtacca ctattaagga
     7741 ccagacagga ttactttgac ttatttaaaa agataaatca accactccag ccgttttatc
     7801 gagcaagtgg cgcacaaatt gattatatac atcaaggggt aggatatgga aataaaattg
     7861 caggaatgga aggattctct cgggttttat ggggagcggg gcccgcaatt gaccaattag
     7921 atgaacgatg gttgaaatta attctatcag gaataaaagc tggaacagat ccttcccatc
     7981 ccgattattg gggagaaata catgaaagag accagcgtat ggtagaaatg cccgcaatgt
     8041 tattagcgtt atatcatcat gaacaattat tatggaaaaa attagataca ggagaaaaaa
     8101 gccagattat tcattggttc tcgcaaattt ttgaatatga atgtgcagat ggtaattggc
     8161 aattttttaa gataattgtc ggaacaatta tccaaaaatt tggttatcca gttaaagaat
     8221 cagcactgaa agaagcattt gataatatag aaaattgtta tcttcaaaat ggctggtatc
     8281 gagacagttc aagaggtcgc caagattatt ataatccttt tgcatttcat tattatggtt
     8341 tgatttatag cgtactagaa ccagaaaagc ccattagcca gatatataag aaccgagcta
     8401 ttgatttttc tagagactat attcattttt ttgcggaaga tggtgcaaat gtgccttttg
     8461 gtcgaagcat gatttatcgt tttgcagttt caagcttttg gtcagctatg ttgtgggccg
     8521 atcttttgcc ttttaaatta ggtgaaatca aggggttagt caatcggaat ataagatggt
     8581 ggatgcaaaa agaaatattt gatgaaaaag gtatcctgaa tattggttat acctaccctc
     8641 aattgacatt gactgaaccc tataatagta cgttatctcc attatggctg aataagattt
     8701 ttttattgtt ggcgctacct gaagaccatg agtattggac ggctaaagaa atgcctatgc
     8761 ctattcgtga aggaactaaa cttctttcag aagccaattt gctcatacaa catgataatg
     8821 gacatacatt ttttttgaat gcgggacaat tagggccaaa tttccataca ttgaccaatg
     8881 aaaaatattt aaaatttgct tattctagtc aatttggttt ttcgataccg cgagcgaatc
     8941 aattgaaagc agaaatggca atggattcaa tgctagggat tcagcgagcg gacacatata
     9001 tcacgaccag ttttaagaat gagagcatca aaacctatgg acagttcatc gttcgaaata
     9061 atgtacgaga cattgtatgt aagaaagagg tacttgcctc tacatgggaa cctactttac
     9121 aggcaaaaat cagaacttgg cttattcctt ttgaaggctg gcagattcgc gtacacaaag
     9181 tagaattaga tacagaatat gttctatatg agacagggtt tgctattgaa tgttctcctg
     9241 agaaagaagg aataatcata gaagaagaca aggaaaacta tagggtgagt gaagctggct
     9301 tttcaggtat tatctgttta agtgacgata ctattaaacg aaaatccaca gctattatgg
     9361 gtttgcccaa tacaaacttg atgacatggg aaaacacttt tattcctgga ttagagggta
     9421 catttggaaa aggaacccat tggctcggaa caggagtggt tgctcatcaa gatcgggatt
     9481 atagtttaga agtatggaac aatcgtccca aaattgattt tgatgggaca accgggctaa
     9541 ctatccaaaa taaaaacaat aaaaaaagac taaaattatg ctaaaggctg gtggggaaat
     9601 gcttgataaa gtgatgtttg aagagataat acatccagaa aaatggataa atgaatgtta
     9661 taaagaatta atggaaaaag aatggaataa tgagatcgag cgttgtcgca gacacacgga
     9721 gaggttaatc aaacgtatca gtaacagtca gtatcaattc ccaagcgagt gtcctataaa
     9781 aggacgttat ccattaagag acaatattgg ttggacagaa ggattttgga caggaattaa
     9841 ttggttgtta tttgagaaga cgggtgatcc tttatttcaa caaacagctg aaaaagcaga
     9901 tatagatttt atccacagga ttgactgtca aattatcact aatcatcacg atcttggttt
     9961 tttgtattct ttatcaaccg tagcagatta taaaataacg ggaaatcgaa aaatgaagga
    10021 agcttcaatt gatgctgcag aaacattact tcagcgtttt tgggagaagg caggagttat
    10081 acaagcttgg ggagatccta gtgatccttc acaacaaggt agaatgatca tggattgttg
    10141 catgaatctt ccattattgt attgggcgag tgaagaaact ggaaatcaaa aatataaaaa
    10201 agctgctcag gctcatattc aaaatgcagc agattattta gtaagagaag atggttcaac
    10261 ttatcataca ttttatatgg atgtcaaaga tggaaaacca agatatggaa gtacgcatca
    10321 aggttatagt gataattcct gttgggctag aggccaagca tgggggattt acggttttgc
    10381 attaagttat cgttatttga aagaacaaaa atattttgat ttaagcaaaa tattagccaa
    10441 ttattttatg aatcggttgc cagaagatac catttgttat tgggatttga tctttgatga
    10501 ttctagcaga gaagaaaaag atacatctag tatggtgatc atggtttgtg gattattaga
    10561 attaatagaa tcaggtctat tagacaaaac agaaaagaac ttataccagt ctgctgtttg
    10621 gaaaattatg cgacaattga aagaaagata tgctacaacg gaagaagaag atggcttttt
    10681 aacacagtct gtttataata agcccaaaaa taatggggta aatacacctt gtatttgggg
    10741 tgattactat tatttagaag ctttagtgag aatgactagt aattggtctt cttattggtg
    10801 aaaaaggtaa atatggaatt tttgtttgat aaaataaaaa aagaaaaacc aaaacagcat
    10861 gaatggtttc tgttttggtt ttttcgttaa ttttcatatt cttctaattc caagcttagt
    10921 tcttcccatt ccgtcaattt tgtttcttga tcttgtctag cagcttctaa atcttggttc
    10981 agctcattga gttttacgtg atcttccagg ttttctggcg cgaccatttc ttgttccaac
    11041 tgatcgatcc attcatcgat ccgcgccatt tcttcttcaa cctgagtaat ttttcgttga
    11101 aggctacgga taagcttttg ctgttctttg ctttggtaat aatcagattt gttcgtggga
    11161 gcaggagcag attctggctc atcttgtgcc aacaattcgg cgatttcttc ttcttcctgc
    11221 tttttctcta aataataatc atagtctcct agatagactt tgctgccatt ttcagagagt
    11281 tcgacgactt ttgtcgcgat ccgattgatg aaatatcggt catgggaaac aaaaagcagt
    11341 gtgccgtcat aatcgatcaa tgcattttct aatacttctt tgttgtcaat atctaaatgg
    11401 tttgttggtt catccaaaat caaaaaattt tctttgttca tcgctaactt ggctaaagca
    11461 acccgtgctt tttcaccgcc acttagcaga gggattggtt tttctacatc ttcgccagaa
    11521 aataagaaag cacctaaagc acttcggatc tctttttctg gagtggtagg atgctcatcc
    11581 cacaattctg ctaggatcgt cttgtttgta tgcagatcag cttgaccttg atcgtaatag
    11641 ccgatggtga catttgtccc tagcgtttct tttccacgga taaaagggat ctggtgaatg
    11701 attgatttca gcaacgtcga tttcccgata ccattcggac ctactagagc aatcgcttct
    11761 tgacgacgga tatctagatt gattggttca gataaggtga cattatcata tccgatagcg
    11821 gcatcttcga tttgcaagac tacatttcca gaaggctttt ctatactgaa tagaaagttt
    11881 gcttgttttt catccccttg aggacgatcc agccggtcca ttttttctaa tgtctttctg
    11941 cggctttgcg ctcgttttgt ggtagaggcg cgaacaaggt tccgagcaac gaagtcttct
    12001 aatttatgga tttctgtttg ctgcttttca taagccttcc attcacttgc taactgttct
    12061 gctttcaaat ctagatattt cgaatagtta cctttgtaat ggcgcatttt tcttctgctt
    12121 aattcataga cttcattgac tactttatct aaaaaataac ggtcatggga aacaatcaat
    12181 aatgcaccag tatagctttg cagataattc tcaagccagg aaagtgtctc gatatctaaa
    12241 tggttcgttg gttcatctag aatcaaaatg tcagggtgtt gcaaaagcat gcgagctaaa
    12301 gctaaacgtg tgcgctgtcc gcctgataaa gtatcgattt tctgatcata aaatgaagaa
    12361 tcgaatttaa acccatgcaa tacggaacga atttcatttt cataaccata gccgtttcga
    12421 tctgaaaaat cgtgttgcag ccggtcgtat tcttttaaat aagaactata agcaggtgtg
    12481 tctggcgtac cttcgctaat gaaaatctct agctcgcgca tccgctcttc catttgtcta
    12541 acttgttcaa aagctttcag catctcgttc caaactgttt cattggaatc aagtcctgtg
    12601 tcctgagcga ggtatccgag agtcgctgtt ttatttttag cgatctttcc ttcatcaggt
    12661 gcttcaattc cagcaatgat ctttaataaa gtcgattttc cagcgccatt tctgccaact
    12721 aaagcgacac gagcgcctgt actgatttct aaatgtatgt tttcaaataa gacttctgca
    12781 ccaaaatggc gagcaatctg attggcttgt aataatatca tggaatcctc ttttctactt
    12841 tgtgaattct cctcaaataa aaaactattt ttcaattagt agtctaccat aaattccatt
    12901 atactgcgag atttgcagaa atgataaata aatgaataaa gaataaaatt tcacaatatc
    12961 tgagcaatgg aattgatttt ttcctataaa tactgctatg atggagaaat caaagatgta
    13021 attagacaat acgtggaggt catttcgtga aagatcatat tattccaaag gcgacagcta
    13081 aaagactgcc tttgtattat cgctatttaa ggattttaaa taatgctgga aagaaaaaag
    13141 tgtcttcaac tgaattaagt gaagccgttc aagtagatag tgcaacgatc cgtcgagatt
    13201 tttcttattt tggcgaatta ggaaaacgtg ggtatggtta tgatgtagaa gatcttatgc
    13261 acttttttgc aaagacatta aacgacgatc aattgacaaa tgttgcgctg atcggtgtag
    13321 ggaacttggg aagtgcttta ctaaaataca aattccatca aagtaatagc atacgtatca
    13381 gttgtgcttt tgatgtgaat gaggaaatcg ttgggcgcat cgtagacggt atccccgttt
    13441 atccaatgga agatatgtca gaacaaatca agatccaaca aatcgaagta gcaattttaa
    13501 cgatacccgc tgaaagagca caagaagtag tcaatcagtt aacggaagca ggaatcaaag
    13561 gaatcttgaa ctttaccgct gctcgattga atgctccgtc agatgtattg atccaaaatg
    13621 ttgatttgac gaatgaattg caaaccttga tctatttctt gcattctgac gcaaatgcgc
    13681 attccgaaaa tacagcagaa taaaaataaa aagcgattga acaaatgaag gcaaaacttt
    13741 cacttgttca atcgcttttt tctgtttgga caacgattat tttgttcgga agatttcttc
    13801 ataagaaagg gaatcatctt ctgctgtcag ttcatctagt agataacgga tttttttaaa
    13861 gtcgatgaac cgtgcttgcc gaaagacttc ttttccttga tgaaaaatca ggacaactgg
    13921 cgcagtcagc acttcaaatt tactagctac ttctggtgtt tcaaatgcat ccaaatgaag
    13981 tgccggaata tcataatgtg tgagaagttc ctctagtcgt ggacgaacag cgtgacaaac
    14041 actgcaatta ggcatcgaaa gataaatgac ggcaacggat gactgttgca gtatgtcttg
    14101 aacttcagtc aagttagtgg tttgtttcat ttttattctc ccttttatga tagaagtgat
    14161 atgtatgaga ccttaattta atgggaataa cagaaaaagt caattgaagt gctttggtaa
    14221 aaaataagag ttgtgtaaaa atttacagag caacttgttt tttttgaata aaagcgtata
    14281 tttgcaaagc gaatgaacta gaaataaaaa ggaggaaact catgttttct tttattccaa
    14341 attcggtttt gcagatgagt acgattttct tatcgatcgt tattgaagca ttacctttcg
    14401 tattattagg ctgtcttatc tcaggcgcct tacaagtatt tttgacccca gaacgtgtga
    14461 agagatggct tcctgaaaat cgtatcttct ctatttttac tggttcgatc ttaggatttt
    14521 tctttccttc atgtgaatgc ggcatcgtac cgatcgttca tcaatttgtt aaaaaaggcg
    14581 taccagttca cacagcgttt gcttttatgc tgactgctcc gatcatcaat ccaatcgtga
    14641 ttttttccac ttttatcgca tttggcaatt cttggcagat ggcaagctgg cgggtgatag
    14701 gcagcttttt tgttgccctt gtgattggtg tctggcttgc ctattttcaa aaagacgctg
    14761 tattgaaaga aaaaatcacg tctaaagtag cagaaaatca ccatcatcat gtccatgaag
    14821 aagaacaaag tgagaagaat gaaccaaaaa atcagctgat ccatcaaact gggcatgtgt
    14881 tgacacattc aatcgacgaa ttttttgata caggtcgtta tttgatcatt ggcggattaa
    14941 tcgcagctgc gatgcagact tatttaccaa ctagaattat gctaacgctc ggctcaacaa
    15001 agattttagc cattatcatc atgctgttat tggcctttac gatgtctctt tgctcagaag
    15061 cagatgcgtt tataggatct tctttgttaa gtctgttcgg cacagcacct gtagctgcct
    15121 ttttggtctt tggcccaatg gtcgatatca aaaatctact gatgatgaaa cgatatttta
    15181 caggacgctt tattttatcg ctgatcggac tgattgccct tagcgttatt ggattcacat
    15241 tatttattta ggagggaaaa tatgctgcgg tttttgattt tatctgggta tttcgaattg
    15301 atgatgtact tgcaagtttc tggcaaactc gatcaatata tcaatgtcca ttatcgctac
    15361 ttagctattc tttcaatgat tttatctgct cttttggctt tagttcagct gtatttgtgg
    15421 gtcaaaaacg gaaaagcaga agaaaaggca cacgagcacg atcatgacca tgggttgtca
    15481 aaaccttatc aacgtggaat cgcttatgta ctgctagctt tgccagtaat cgtggggacg
    15541 ttgtttccaa cagttagtct cgacactacg atcgtagaag ccaaaggatt taatttccca
    15601 gtaagtaaag aatcagtagg ggatccggag atgcagacac aatacttaaa gccggatacc
    15661 agtatttatt tcaacaaatc agactactta gaccaaatga atgagttgaa agaaaagtat
    15721 ggggataagc aaactatcaa gatcacagat gaaaactatt tagaagttat ggagctaatt
    15781 tataattacc ctagcgaatt cattggcaaa aaaatcagct atgaaggatt tgtttaccaa
    15841 acaccgaacg atagcaatac agatatcttc ttgttccgat tcggcattat ccattgtgtg
    15901 gcagattcag gagtctttgg tttgttgacg catatgccgg aaggagtaac ggttaaaaat
    15961 gatgaatggt ataaaataga aggaaccatc caatcagact attatcaacc attcaagcgt
    16021 gaaatcccat ctgtagttgt tacaaacgct gagaaagtct ctgctccgaa aaatcagtat
    16081 gtgtatcgtt ctttttaaaa gaacaaaata aataaacata gctcagacag ctgacacgca
    16141 aggacgtcgg ttgtctggct ttttttgcat gatgtattca tttgtttgat atttatttta
    16201 gaagcagcat cctgaaagta tttgatatcg gtttcaaagg tgttggaaaa gattgtgaag
    16261 agaattacaa tttattccta acatgtatgt tgaaaagttg tatgaaatga ttttgatagt
    16321 aaaatatcga gtggaggtac tttttatggt aacgcgtata tttattttgg atgatagttt
    16381 ggtttttgaa aaaatgctag tagaaatttt ggaagaatcc agaattttat taagtccttg
    16441 gaaatttgag atcgttcagg gattgaacgt tatacaattt gttgaattca ttaaaaataa
    16501 tgaaatctat tcctcagata tttttttcct agacatcgat ttgaacgcct attatagcgg
    16561 tatcgaacta ggcgaaatgc ttagagaaaa gaatgaaaag tgtaaaattg tttatctaac
    16621 gtctcatccg aataaagcaa taactgttat taatcgaaag attacgccta gtggttattt
    16681 gataaaagaa ttcacttatg aaaaaaataa aaaaagcatc caaagttact tattgaaaga
    16741 aaaagaaaac tatcctgttc aacatgctac gaaaaaaagg tggacaactg tccgttatgg
    16801 taatagcgat cactatatcc attacgatga aatcctttat ctaatgagca tgccaggtta
    16861 taaaaataaa ataggcctag tattgaaaga tgaagagatt ctcgtaaacg gcacaattaa
    16921 aaaatataaa aaatcactcg aatatgagta tttatgtaaa gatttaaaag cttatatcat
    16981 taatactgaa aacattacat ctattagcag agtaaacgaa atagtaacat ttgcaaatgg
    17041 agaacaacta gagatgggaa agagaagtat tgataaacta aagaatttta tgcaagaaaa
    17101 gatagaaaat gaatcatttt aaagaaaaca aagactcata tcttacctgt tagaagagtg
    17161 ttttcctgta gaagagaaca acccaagtag cagcctagtc gtttctatcc ttttttgtta
    17221 tgcactcttt ttgacattta ctccaccaat aacgaagggt acaataaaaa gtaataacaa
    17281 acaaaaggag tacagaaatg attgaattca tcaatgtaag taaaagttac gggaaaaaac
    17341 aagcattaaa agacttgtct ctctcgataa gacaagggga aatttttgga tttttaggac
    17401 ataatggtgc cggaaaatca actacgatta aaagtttagt tagtattatt gaaccatcat
    17461 cgggaacgat tttagtagat ggaatgaaat tgacggaaaa tcgattgagt atcaaacaaa
    17521 aaatcggtta tgtgcctgat tccccagata tttttttaca attgacggct ggagaatatt
    17581 gggacttgat ttcagctgcc tatgaattag acacacaaaa aaaagaaaag cgtttagcag
    17641 aattgacagc gctttttgat atgtatagtc atcaaaatga aacaatcgca agtttttcac
    17701 atggaatgcg tcaaaaaacg attttgatcg gcacgttgct gccagatcca gatatttggg
    17761 tattggatga acctttgcaa ggacttgatc ctcaagcagc atttgatttg aaagaaatga
    17821 tgaaggctca tgccgcaaaa ggaaaaacag tcattttttc tacacatgta ttggataccg
    17881 cacaacagtt atgcgataaa ctggctattt tgaagaaagg tgaattgatt tatcaggggg
    17941 cagttagtga attattgaat ggctcacctg atgaaacgct agaaaagatt tatttgaaaa
    18001 tggctggaag gcaagcgaca ggagatggac cgtatgtata agcgacaatt attagaatta
    18061 attcgagtta atcttttata tgttaatcca cagctcacga ataaagcaag agagaaagga
    18121 aaaaagggaa agcgattaac tatctatctt ttgtctcaaa atcttttttc agggctgatt
    18181 tttttactga tttacggatt tgctatgttt gctatgaact ttgttaaatt gccaggtttc
    18241 tttacttatt acgtggcact ttttggtatg ttaggtttaa ctcaaagtat atcagtcatt
    18301 tataatatct tttttgaagg aaatgacctg caagtatttt tgcctctgcc ttttggacaa
    18361 gaacaggttt tttcagcaaa gattttggta gttgctttga ccatcattcc ttttgttttt
    18421 ccgcttctag tcatgtttat tttaaccgga tggcgagctg atctagggat tttgcttact
    18481 atcattatct caatcctttt gttttctcta tttttgatta ttcttttttg tctggctagt
    18541 ttgattgttt ttggtttggc tcgaacagca gtgtttaaaa aacatagaaa aacagtttct
    18601 agtatcttgc ttggcagctc gatgctgatc gcatttgctg gaatcatgtg gatgaatcat
    18661 caaacgggga ctattgaaaa tgagttaaca gatcggcatc cgatttcgat attattgcca
    18721 ttctatgatg ctgccagtca gccactttct caacaaggtt tattgggttg gggaatacta
    18781 gctatcacgg ctctgggatt tttccttgct gtaaaacagc tgatagtacc aaagcttaca
    18841 gagcaactga ctgattcaaa cagtgagttc aaaataaaaa gaacccataa aaagaaccaa
    18901 aatgtgcatc agcttttatt tagttacaat gctcaattag taaaagaacc taatttgatt
    18961 atgcaagttc tttcttcttc cttgttaact ccaatcattt ttattcttgc ttttgcccta
    19021 ggaggcgaaa taaaattaac tgatttagat aataagttaa tcggtgttgt tttcctggta
    19081 gggatcgcat tggctttctt aacagttaat cagacttctt ttatcagcaa tcttatttct
    19141 ttggatcaag aaaattttct gtttatccgt tctttgccaa tctctatgaa ccaatattta
    19201 aaagaaaagt tcagattcgg atggatattg cagagttttt taactggtgg gatcgcatta
    19261 ttaagcggac tgtcttttca gctgcctttg ttttttatcc ctagcttgct atttggttct
    19321 ttattcggct gctacttatt gtcactacgt tatttcgcta gagattatcg gttgctgctg
    19381 ctgaactgga ccaatattaa ccaattattt aacagaggtt caggaagttt gggtttagtc
    19441 gcaactatga tgggttcatt gatcatcagt attattctta tcgtcttata cggaatgttg
    19501 gctttgtttt atccattttg gttagtgaat attcctgtga tcggtttagt tctaatactt
    19561 tccgctctat ggatcgttta ttatcaacga acgttttgga aaaaatttga ataaagaaga
    19621 ggggccgtga caaaaatcat gtcgcagccc cttttttaca gatagagggt gttcaaaagt
    19681 cagtttctca gtattaagtc aattttatgt aaaaatgtga gaagctattt ttacaaaact
    19741 ttttttaata ctcataatta cttcttattg ctcctgcggt tacttgtcgc aatgtttgtg
    19801 agatattttg tcatcgctca aggctgaacg tctctttcac aaccttttct ttgttacact
    19861 tcgtttattt aaaaagccgt ttttttcgtt tcatcatgat tgcgtcacgc tttactttga
    19921 aattacggat gttatggtta aagacttctg ataatacaag acctaaagcg attgctccag
    19981 caatcataat tgcttggacc gcataatgag ccgcttctcg ataggattca gtaacgagat
    20041 ttcgaaccgc ttgataagct aaaccaccag ggactagagg aaccatcccg ggaatattga
    20101 aaatcgttac gggcattttc aaacgtttag agaaaagatc gctgatgaat gctactccta
    20161 aagaaccaag caatgaacca aaagcagcag atccgcctat ttgaacacag atccagtaga
    20221 tcatccagcc aaaagcacca gaaagaccac aacaaatcaa tgaacgccta ggtacatttg
    20281 taataacggc aaatgccgca gtcgctaaaa aactaaaaga aaactgaata aatatcgtcc
    20341 ccatacttgt gttcctttcc taataaaaaa gctggaaaat aaaagcaatc gcaaaaccga
    20401 tcatcgtagc agtcatcatc gcttctgttc cgcgagaaac tcctgagaca taatgtccag
    20461 ctagcaaatc acggatagcg ttcgtaattt gaactccagg aactaaaggc atcacacatc
    20521 cgataatgat tagatcctga ttgcttccta agccaaaacg tacgctgtag atagcggcta
    20581 tgccaatcaa taaagaagat aaaaattccg ataaaaattt gattctaaaa aatttcaaac
    20641 tatatagata agctgcgtat cccaatcctc caatgactaa agtcacaggt aaatcggtca
    20701 tctttccgcc aaataacacc ataattgttc cactgatcac tgccgcgcta acgatttgca
    20761 accagacagg aaagaaagaa cggtctcggt cgattgcttg cagttcgcag taaagttctt
    20821 ctaatgttag ctctgaagca acatatttac gtgaaagatc atttactttt actacttttt
    20881 ctaaattaat cgaacggttg gtaatttgtt ccattcggat ggtcgaggta cgatcaagac
    20941 caataaagag accggtttga gtgacatagc tgaccaaacg gaaatttcca gaagctaaag
    21001 caattcgact catcgtatct tctacccgat acatctccgc attgctttcc atcatgattt
    21061 tcccagcgag taaacaagtg tccagcaaca gatctaattc ttttcccgtc atagcatctt
    21121 ctccaataat tgattgatta gttttatttt actacaaaac ataccagaag cgtaaagaat
    21181 aaaaaaactt gaggaaggaa taaaagaggc gtatactgtt tttgtattgc gggtgtagtt
    21241 tagtggtaaa atcccagctt cccaagctga tgtcgcgggt tcgattcccg tcacccgctt
    21301 aaaataggtt gtgaaaaagc tgctctgcaa caagtaataa gaacagccaa gcaaaaacaa
    21361 cttcttatgt ttttaacttg gatggactta ttaccgcaga tgcaagcttt tgcgcaccgt
    21421 ttaccaagcg gttgtgaaat cagtgtattg tcttgagtat gaagaaaatt tttgaataat
    21481 aataaatacc ttacagatag agtggtgact gattagagac gaggaatccg tctctttttt
    21541 gtatttttaa gccatcttaa aacaggtttt attttttgct gtttctattt ctttctagta
    21601 tttatttata ggggaacata tgttaagatt tagggagctg ttagtaaagg agtaaagaaa
    21661 tgagcaaaaa aacaaccaag gcgatcgtgg ggatcgtcgc tgctgtgatt gcaggggcat
    21721 tcggtgtttc caccacacaa aaagattcag atatctttca gcagattaca actatttttg
    21781 attccacacc acaagcttct caagtagcgg atgtgccaga atatgatgga aaacatcaag
    21841 aaatagaaat caatgggaat cagccggaat ttacaaaaga ggatcttagc ctttccaaag
    21901 gaacatggga aagctattca aatattgata aattgaacag agtaggacaa gccaatgcta
    21961 tgcttggaaa agatttgttc cctactgaaa agagagagcc tttgtatatt gacccgacag
    22021 gttggaaaca aaagaaatta agtgacgggc aatggttata taatcgcagc catttaattg
    22081 gtttccagct gactggtcaa aataacaaca ttaaaaactt gatgacaggg acacgttcgt
    22141 tgaatgctcc atacatgctt gctcacgaaa atgatattgc ggcatatatc aaagaaacaa
    22201 atcatcatgt acgctaccgt gtcactcctc attttgaggg gaatgaactg gtagccaggg
    22261 gagtgcaatt ggaagcagaa agcattgaag acaaaaaaat tgaattcaat gtctttatct
    22321 acaatgttca agatggctac acgatcaatt atgagacagg acaagcaaca aaaacaaaaa
    22381 attagtagca gctattcgaa ttttttatga atattatgaa aaaattgtgt aaaaacatac
    22441 attcaaagaa atatagcgta tactttatga aaagagtact tttgataaag ctacagaaaa
    22501 aaggaaaaaa acatacaaat agtgtttctt aatttattcg gaagtttgga atatttgttt
    22561 gtagttttcc ctttcttcga gtaaaataag gttgtagaag gtaagctgga attaccttgc
    22621 gggaaagagg tgtagaatca tgggttttac agatgaaacc gtacgttttg gttttgacga
    22681 tagtcgcaaa aaagaagtga gcgaaacatt agcgattgtt tatcgggctt tggaagaaaa
    22741 aggctacaat ccaatcaatc agattgtcgg ctacttgctt tctggagacc cggcctacat
    22801 tcctcgttat cgtgatgctc gaaatttgat tcgccgtcac gaacgcgatg agatcatgga
    22861 agtaattgtc aaggactacc tctcaaatca tggagtgaat ttatgagagt aatgggcttg
    22921 gatgtcggtt ctaagacagt aggtgttgct atcagtgacc cctttggctg gacagctcaa
    22981 ggagtagaga tcattcggat caatgaagca gaagaagagt ttggctttga tcggttgaag
    23041 gaattagtag cacaatatga agtagaccgg tttgtagttg gattacctaa aaatatgaac
    23101 aattccatcg gaccaagggc agaagcttcc atgtcatacg gagaaaagat caaagaacta
    23161 ttctccttgc cagttgatta tcaagatgaa cgattaacca ctgttcaggc agaacgtatg
    23221 ctagtagagc aggcaaatac ttcaagagcc aaacgaaaaa aagtcatcga taaattagca
    23281 gctgtgatga tcttacaaaa ttatttagac ggctcaggta agcaattatt ttaataataa
    23341 aagaaaagag gtaatcttat gacagaacat aaccattccc acgaccatga aggacacgaa
    23401 cacatcacat tggtggatga ccaaggaaat gaaacattat atgaaatcct actaacagtt
    23461 gacggacaag aagaatttgg acgtaactat gttcttttat atcctgctgg tgtttcagaa
    23521 gatgaagatg tagaattaca agcttatgca tacatcgaaa acgaagatgg aacagaaggc
    23581 gagttagaac aaatcgaaac agacgcagaa tgggacatga ttgaagaagt attcaataca
    23641 ttcatggcgg aagaagaaga atagaacaaa agaatgaatt aataagatat aaaaaaaggg
    23701 attgtgacaa acgtcttgtc acaatccctt tttccaaata aacgatgttc aatcagctgc
    23761 ccctcggcat tcgctcgaaa aacggcgaaa tatgaagaac tattttgcca tttgtttctc
    23821 taataatcag gtcaaaacgt tgacttcaca gtctcttcct taaacggtgt tctcctgcca
    23881 actcctcggt cacaagtcac aatattcaac aaacatgaga agctgttttt tgaaattgtt
    23941 ccttgtgact cggagctgaa cggcaggctc acgacctctt tcttaaacgg tgttcaaaag
    24001 cttgcatctg cggtaataag ttcaaccaaa cgaaaattat gaggagctat ttttgtttgg
    24061 ctgttcttat tacttgatgc aggacagctt tttcacaacc tcttttcgtt tagcactaaa
    24121 tttccttaaa aagtagaaaa gctttcgaag cctcgtttgt ttcttttact gtataataaa
    24181 agaaaagagt aacaatcgct aaaagatata aacaagagaa aggaaataca agatgattgg
    24241 tctgatttat tttgtggtga tcgttctggc gaacacagtt ggcgctattt ccgggatggg
    24301 tggaggcgtg ttgatcaaac cgattcttga tcttatcggc gcccattctg ttgcagggat
    24361 ttctttttac tcaacagttg cggtattcac gatgtccatt gtttcgactg tgcgacaagt
    24421 atcaagcggg aaatcactga attggcagat tgtcggttgg gtatctagtg gagctgttgt
    24481 tggaggagta gtcggcaaca ttgtattcga agttttttta cagttatttg aaaacgagaa
    24541 gcatgtgcag ctgatccaaa tttttttgac ggtattgacc ttggtttttg cgttcttcta
    24601 caccaagcat catcagccaa aatttcattt gacgtcatgg acttggtacc ttatatgtgg
    24661 gggggttctg ggctttttag ctagtttttt aggtattggt ggtggaccag tcaatgtttc
    24721 tttgttgatg ctgatgtttg cattgccaat aaaagaagca acactatatt ctctatctac
    24781 gattttcttt tctcaactgg caaaattggt aacgattgct ttaacaagta gctttatgcg
    24841 ctttgattta tctatgttgt tttatgttat ccccgctgct gttattggag gtttgtgggg
    24901 agcaagattt agcagggttt tgtctccaaa aaaagtgacc tttatttttc aagtaattgt
    24961 cctcgtggtt cttttggtca attgttacaa tgcctttgtc atcttacaaa cattaggaga
    25021 atctagacta acgcaagtca aaatcactga aacatattgt tctaaatgaa aaaaagagta
    25081 tactatgata aaaaaattca ttttaaaaaa tcagaaattg gctggtgatt tggatgttac
    25141 gggaaatgcg tttagaagat gcagaagcac tacgtgaaat caatgcggat caattagggt
    25201 atgatatacc attaaccgtt acggtgagac aaatgaagaa actgctgaag gagccagaga
    25261 aaaatttttt cttggtttat gaagatccta cttctaaaaa agcagctggc tatgtacaag
    25321 cggagtccta tgaatcgatt tttagcgatc caatgttcaa tattatggca cttgctgtgt
    25381 ctaaatatgc tgaaaaaaga ggcatcggaa aggctttgat gactggcgta gaagaagcga
    25441 aaaaacgagg aataacgaca ataaggctta attctgcaga atacagagta gaagcacacc
    25501 aattttatga acacatcggt tatcattccg ataaaatgca aaaaagattt ttgaaaatcc
    25561 tgtaaatctt tatcaaaaaa atacccgatc accaagccga tacttgatga tcaggtattt
    25621 ttgttttttt gactaagaaa gtaattgcat caagaaacca gagatgatca aaacgatggc
    25681 accaccaagt cgattgccca tttgagcaaa agcaataagt tccatccgat tagctgcaga
    25741 taagacggct acatttcctg ttccgcccat cgagttgttg cataagccag cagtaatagt
    25801 tgattcaatt ggatataaac caaacaaacg accgacaaat cctgaaacaa ctgaaatcgc
    25861 gataacactt gtcagacata agacaacaaa ttgccatgtc aaagcagaag ctaatacatt
    25921 cagattcagc aacgcaatac cgataccggc aagaacagct gctgtcaagt ttttgacaat
    25981 caagttattg aacatgatag ctgcatcttc ataatactct ggtaataggt tggcgatttt
    26041 acagatgaca acgataataa tcataaatgc gtaagcatgg actttaggaa caaaatagtt
    26101 accaatttgt cctaagatga agaaagtcaa tgataccatc aaaccaacac ctaactgtgg
    26161 aatggaaggt ttcacagttc gaggttttcc atcgccaaga tcgccttttt ccattaattt
    26221 accattgccg ttgaattttg gcattgattg tccagcacga gcaactaatg ctgcaccgat
    26281 gatagctaag acatttgtca ttgctgtcgc aggaatcagg cgggaaatga tagaagaagc
    26341 atccgtacct aaaacatttg cataaatcgt tgataaagga acggctccgg ctccaacacc
    26401 accagccatc gcaggtaatg agatgtataa aacggaattt gcaaaaccat ttccgataag
    26461 catgcccacg agtcctacgg caaaaaagga gacgaccatt gaaataagtg caacgggaat
    26521 aaaacgaaca gaagctttca tcaatagatt acggttcata ccaaggatgc taccagtaat
    26581 caaggcagca atataaaagt ccaaaaagcc catattgctg acaaagtttt ctgtggattt
    26641 cactacatat tcaggtaaga tcccaaatgt agcaatcgca gcagaagcga agatcgcaaa
    26701 aacggaacca ccacccaaat aacttttgac gatagggatt tttgtcccga taaaatgaaa
    26761 taaattaccg aaaataacca aaactgcgat tggtccaatc atattcgtcg gtaatttccc
    26821 taaagccatt gcaatgatca ggacaaggac catgatcaaa taaagtggca aacttacgcc
    26881 ggtgattttt gtggaccaaa aaccttcttt tttcttttcc tcaactgcag ttgcagttgt
    26941 tgttttttgt gccattttta cagatccttt cttattctgt atcatatgaa aaaagccaaa
    27001 gaaaagcgac aaaagagcag gagttggctg gccgctgttt tgccgcagtc gcttttttct
    27061 ttttagacgt catacaaagt ttctgtatgt tcttttacta taacgatctt aatcttctaa
    27121 agatttatat tcaggaaccc atttagcaga ttcaactgct tctttgatat ccgtgatttc
    27181 ttcacgattc aagttttgtg cgacaactga ttttgcgaca gtttcagcaa tcgtttgaga
    27241 aaattcagtg atttttgcta ctggaggcaa gatagctgct cctggttttg ttacatcaac
    27301 gattccgcct aatgctcgac tagcttgaga aatgatttca gcatttactc gtgtagctgt
    27361 tgaagcaatc aaaccaagtc ctaaacctgg gtacatcaaa gcattatttg cttgaccgat
    27421 ttggtaagtt acaccattat attcaacatc agcagcaggg atacctgttc caactaatgc
    27481 ttttccgtcc gtccattcta ttaaatcttt tgcttttgct tctgccaatt ttgttggatt
    27541 agacaaaggc atgatgatcg gacgtggtgt atgagcagcc atttctttca cgatttcttc
    27601 agtgaatgct ccaggttggg tagaagtacc aatcatgatt gtcggatgga tcgcttttac
    27661 aactgcttct aagtttgtca attcatcagc attagtgaaa tctgagcgtt tgcgagcgaa
    27721 tgggatttga ccaggagtca atccttctgt atcttcaaac aataaacctt gtttatctac
    27781 ttgatagaaa tgttggcgtg cttcttcttc aggtacacct tgacggatca tctcatctaa
    27841 aaggatgttt gcgataccca caccagcagt accagcacca aatgtcaaaa ttatttgatc
    27901 tttcaattgt tcaccggata tattcaatcc gcctaataca cctgctagga caacaattcc
    27961 tgtaccttgg atatcgtcgt tgaaagtagt gatttgatct tcgtattttt ctaagatcgc
    28021 tgcagcattt cctcgtccaa aatcttccca atgcaataac aattctggga ataattcggt
    28081 agctgattga acgaattgat cgatgaattc atagtaagtt tcgccttcta cacgagcatg
    28141 acgattgcct aagtataagg gattattcaa taattcttca ttattagtac cagcgtcgat
    28201 cgatacaggc aaaacttgtg ctggattaat accagcagca gcagtataga ccatcaattt
    28261 gccgatagcg atgtcaacac cgttcacacc ccagtcgccc atacctaaga ttccttctgc
    28321 atcagtcaca acaatcaaac gaatgtctcg tccatctgcc gcgttcttca aacttgcttt
    28381 gatgttttct ggttcgtcga ttgaaagaaa tgcagcatct tgcggtttca agaaaatttc
    28441 gttgtattgt tcgattgaat ccgcaacgac aggatcataa acgacaggca tgaattcaac
    28501 taaatgttcg cccatcaatt tataaaacaa tgtccgattc gtattgaata gattcattaa
    28561 gaagatacgt ttttccaaat ctgtttgttt gcttaagtat tgaccataag cttgaacaga
    28621 ttgttgttcc agtgtttgga cagtacttgg aagcatacca gtgattccat attttgcccg
    28681 ttcttctttt gtaaaagctg ttcctttatt tagaaaagga ttatttaata agtttaaccc
    28741 tgtaagcatg aatgattcct cctaagtttt aatacaaacg aagtatagaa gtcagatgat
    28801 gatatagtca aaaagagcag gtatgataaa ttttatttat atgtaatata gccaaaagaa
    28861 atcaaaatag cagtatttgt tctttttata tcgacaatcc ttgcttgaat actatgcgta
    28921 tctcttatat gctagagaaa gaaaagtaga agaataaggg atagatggaa tggataaata
    28981 ggatttctga ggaaaaaagc acatgatagc gttgatagga gtcacctatg tcaggtgata
    29041 aaaatacata agtagaaaat taagaggagg aagagaaaat gaaagaagtt caattgatta
    29101 gaaaaagcga actatcagaa ggcggttgca atgcatgtgg cgtagtagaa gcgacaagct
    29161 atacattaaa attagattcc aatcaagcaa taatctctga actgacagta ggcggtcttg
    29221 tggacagtct ggcgcttgct gaaggtttta ctggagaaga tatttatgag atgttttcag
    29281 aagtccgtca gttaaaaaaa ggagaaaaat gcatagaggt tcatcatgag agcccgaatg
    29341 tgaggttcaa acgtggtgat aatgagatga tcttcaagaa tcatgtgtct gatcacactg
    29401 aactatatga aatcgtgaat caaatactgt ctgggatttt tgaattagga ccttatgagt
    29461 tcaaggaaga aaacggtaat ccaaaattaa atgaagaatg gcaagaaacg attgaaacac
    29521 aaagaaacaa tccacactta tttcaatagt cttccacagt atttcgtaaa aatgttgatg
    29581 aaaaagaaac accgtgaatc agaggtcgtg aaaaacctgc cctgcatcaa gtaataagaa
    29641 cagccaaaca aaaatagctc ctcctatttt tcgtttggtt gggcttatta ccgcagatgc
    29701 aagtttttga acaccgttta aggaagagac tgtgaagtca acgttctgac ctgagtatta
    29761 gagagacaaa tggcaaaata gttcctcata ttttgccatt ttttgagcta ataccgaggg
    29821 tcagctgatt gaacaccgtt ttatcgagag gttgtgaaaa agtcgcttag tttcgagtag
    29881 taagaaaaat tttgtgaaaa tggctttcca catttttgca taaatttgac ttaataccga
    29941 gaaaatggct tttgaacacc gtttattcaa aaaaagggac tttgacacta cttttgtcaa
    30001 agtcccatcg gttttttatc tatttttatt acaatcattt cttccagagt agtgacatct
    30061 ggaatggctg aaatgtaaac agattatttg cctgtatcaa tgtcgaagag taatataaaa
    30121 agtgtaaacg ctatcatatt gttcatcatt caaattttat agagagaagg cattgagatg
    30181 gaaaaaacat atcaagcagg tacaaatgaa ggaatagtag attttatcaa tatggaagat
    30241 ttagaaatcg cggcttcaca agtgattcca gctggtggtt atggttacat cagcagtggt
    30301 gctggcgact tatttactta tcaagaaaat gaacgggcat tcaatcatcg tttgattatt
    30361 cctcatgtat taagagatgt agaattaccc gatacaacta cacattttga cgaagaaatg
    30421 ctgactgcac caatcatcat ggcaccagtt gctgctcatg gattagctca tgtgaaggcg
    30481 gagaaagctt ctgcaaaagg tgtagcagat tttgggacga tctatacagc aagttcttac
    30541 gcttcttgta ctttagaaga gataagagaa gcaggcgggg agaaagcacc gcaatggttt
    30601 caattttata tgagcaaaga taatggaatc aatttggata ttcttgaagt ggccaagcgt
    30661 aatggagcaa aagcaatcgt attgacagcg gatgcaacag ttggcggaaa ccgtgaaaca
    30721 gaccgcagaa acgggtttac cttcccgctg ccaatgccaa tcgttcaggc ttatcaatca
    30781 ggagtaggcc aaacgatgga tgctgtatat aaatcttcca aacaaaaact tagtccaaaa
    30841 gatgtagaat ttatcgctgc tcattctgat ttgccagtct acgtgaaagg tgttcaatca
    30901 gaagaagatg tctatcgatc attagaatct ggtgctggtg gaatttgggt atctaaccat
    30961 ggtggccgtc aattagatgg ggggcctgct gcgtttgatt cgttgcaata tgtggcagaa
    31021 gcagtagata aacgtgtacc aatcgtgttt gacagcggtg tacgacgtgg acaacatgtt
    31081 ttcaaagcaa tcgcatcagg tgccgatctt gttgctattg gcagacccgt tatttatgga
    31141 ttgtctttag gaggaagtac aggagtccat caagtgtttg atttctttaa aacagaattg
    31201 gaaatggtca tgcaattagc tggaacacaa acagtagaag atatcaaaaa aataaaattg
    31261 cgtgagaatc gttttattta agaaataaat gacgaaaaaa ataggagtgc ggcagatgat
    31321 gccgtactcc tatttttata gttcaaggat gtcactaata gaataatttg aaacgaaaaa
    31381 ataaaattga aatatttttt catacttaat aaaaaaggaa gaatattcat gtaaacgatt
    31441 ttattgtttt tttctctttt atacaaaaag ggtcataatt tcaagtaagc ggatgaattc
    31501 atgaggattt tttgcatact gtctctacta tacttaggtc actaagaaaa aggagtgatt
    31561 tattatgaaa aagaacatga ttgggacatt attactttca acacttcttc taggcggctt
    31621 ggcaacacca gcttttgctg agggtcaagc gacttcaaaa ggagatatta cctttactga
    31681 accaacaaat accgttgagc ctcttaaccc tactgatcct tcaaaaccag tagagccagc
    31741 agatccggaa aatccagcaa ctggccagac aggttcttta acattggatg tagttcctga
    31801 attgccattt ggtacacacg agattgaaag cggaacaaaa acgtatcaag tagacgcttc
    31861 aaaaaacgat acaccttatc tccaagtatc agatcgtcga ggtgtaggtg cagatggcca
    31921 agcacaaggg tggaacgtga cagtttcggt ttcagatttt gtaaacggtt cacaagtttt
    31981 gcaaggcgct gaattagatt ttggaacatc cactgttaaa agtacgtcag ataacgaatc
    32041 cactggacca actagtcaaa ctgtaactgg gttgagtaaa gcaagtgcag cgacaccaat
    32101 atttacagca gcaaaagatc aaggtctggg aacatggctt tctgtttatg atccagcaaa
    32161 tatcacactg aaagtgccga aagcagcagc tggaacattc acggctgatt tgacttggaa
    32221 cctcgtggct ggcccagtag catagagtat gaaataaatt caacaaactg cggtgaaaat
    32281 actataggtg aaggattttt taccgcagtt gttgttgaaa ggatgtggtg gacaatgaat
    32341 caaagtaaac gactgtttgt ttcgtttttt tctattttaa gtctattttt catttttccg
    32401 tcaatcagcc aagcaaagga ttcagcagga gactttggaa tcaaaccggt atttccagag
    32461 aaccaaatag ataaagcaat cggttatttt gatttattag tggcaccgga acaaaatcag
    32521 acactagaag taattatcag caattcttca gatgaagaac gaacatttga ggtctctgtc
    32581 aatccggcgg taacgagtga tggtggaacg atcgattata gtcaaaaaaa cccaacgtta
    32641 gatgagacat tgccgtttga tgtccgtgac gttttgctca ttgcgaaaaa ggaaatcaat
    32701 gtatcagcac atgcagaaac aactatacct atagaagtga aaatcccagc aaaaagcttt
    32761 aagggacgtg tattagcggg tattcatgtt tcacctaaag aagaagctga aacagaaaat
    32821 gctacagaag gagcacaaat caaaaatcgt attgcttata atctcgcagt cgttttacaa
    32881 gaaagtcaag agacgataga accggatctg aaacttttat caggagattt ggacgaggtc
    32941 aacgcaaagc caactgttca gcttcgtttc caaaatcctc agcctaggat tatcagcaat
    33001 ttgatattta cgagcaagat cttttatgaa aatcagcttt atattgaaaa tacgtctaat
    33061 gaatttttag ttgcaccaaa ttcgaatttt cacttgaatc ttgatttagc tggtgataaa
    33121 gcaaaagccg gcgattaccg cgcagaaatc atagcaaaat caggcgacag taatgaatgg
    33181 cgatttacac aaaactttac gatcaaaaaa gaaaaagcac aaaaagtaaa tgaaaattcc
    33241 gtttttgcag tacaagaaca atctttccca tggctttatg caggattggt tgctgcagtt
    33301 ctagtgttat ggttgatttt gctgtttttg tgggtccaca agaaaaagaa aaaaggagaa
    33361 acaacatgat gaggtgattt cgttgaaaac atcgatctta ttgggaggga ttattgcaag
    33421 tttgctgttc ttcagctggc aagagccagt ccaagcagat accaaaagtt caggtacgat
    33481 tacattttat gatctaagtc cagttccgca tccaagtggt tcagtcaatg gcgcatcaaa
    33541 acagcagaca cagctaccga tttataaaga atctaatcaa catggcgtat tcccggagac
    33601 gaatgaagaa aagatccgtt ttctttattt gatcattggt atagaattat ggcttcttgc
    33661 gtggttgttg ttatggagaa gaaaggaaaa ggaggatgaa catgttgcta caaaataaat
    33721 tttcattttt tcgctccttc ttttttactt cgctttttgt tttaagtata tttatctttt
    33781 caccttcggc agaagctgtt ggagcaacgc ctcctgccat ttcttatgat ccatacacgt
    33841 atgaaaatat cgtacctcca aatggtggac gtagtgcagt caatggcggg aagcttgtcc
    33901 aactaactgg ggctaaccaa ttgcctaaat tgactgctac ttatttgaac gatggttcaa
    33961 atgatccttg gaaaggtggc acggtccgtt ttcattggga aagccatgtg aaactaacaa
    34021 atgtacttga tttggtgaat atcactgcac aaggatttgc atttgtggcg atcatcaatt
    34081 tgccggaaca tgtatcaagc agtgatgtat taaaagcaat tgactggaac aacgcctact
    34141 tgtatattgc cggtgccccc atcaaggtta ctagcggtag tttattatca gcaggaagcc
    34201 ataccttgcg ttttgggatg gggtcgtggg atacaaatac tctattggca gatattattg
    34261 ctattattac gcgaggcgaa ttcaacgtgg acgatatccc gcttcaattt gatgttacag
    34321 ctaatgtcgc gaagatgaca gaaaacggag ataaatatga tacgagtcca aacaaaattt
    34381 tgaccaaggg taaattacaa ccttctatgt ctaaaaaggc cgttttttct gttgattttt
    34441 atgattcgaa taatattatc caagatcgta tgttcaattc gattttagct ccagctgcag
    34501 gtagatttta tcctaaatta actggcggaa acttgaatcc aggagcatat gcattttctg
    34561 caagtgcaga gataaatacg tggaatagtt acatctcaac aagtgattca acaggaaaat
    34621 ataatacatt tgctgatacc acagaatacg tggatggaaa taaccagaac cttccaggct
    34681 ctaccaatag tcggacattg acgatggggc tgactgaagg acgttttgat cagttgccat
    34741 ttagtcgctt tgatcgcgta gtcaactatt ttacaggaaa aaacgttaca caaggagcaa
    34801 tactgactca tactccagca agcacaagtg gattgaatca gacagccaat atcgtgtaca
    34861 gtgggaaaga tgcgcaaggc ggattgcttt ctcctgttgc tttaaaggta ttggagcagt
    34921 acaagctaga cggaacggtt ttaccaacaa acttaacagc cgatagtcag tggggaaagc
    34981 tcctttcttc tgcatcgtac caagtcacgg ccaattggtc agcaactgaa ctaaaaaagg
    35041 gttcgatcca ttacaggctt tattcgaaag gaacccaaaa attagcgaca ggatggacag
    35101 atcaactttt ccaaacgatc caatcaaata acgggggtgt aaatacagct acttctacac
    35161 tacctgcatt ggcttcaggg caatattatt ttgattatcg cttggtggac gatgtactta
    35221 ctaacatgta tccgtctttt gcgtacaaat ggcagtctga acaagctgga atcaccactc
    35281 ttcctcaaat aacagtggct aattttccat ctgtgagtgc agatagtcag ctaaaaaacc
    35341 ttagcagaca acctgagaca ggagatcccc tgtctgctct atcaggagat acgattcaag
    35401 aaaatgtgac attcacgtta acaaaatctg gggatgcgat aaccgacaaa aaaatcacga
    35461 tctcacttcc tgaaaatact acttatatca atggttcctt aaaattaaat ggtacaacta
    35521 tcgcagatac cggcattcaa caaggagttt ctgtcccagc agatctattg agcaaaatag
    35581 gagataccat tcatcttact tacaactatc aattaaatac tgtagatact tctgtacaat
    35641 ccgtcagcat actgacaaag gcagctgtac tttccagcaa tattacatta gcagatggtg
    35701 ctaagcttcc taacccagtt gtccaaacat ctgctaaaac aattcttgtc cccaaacaag
    35761 aattaacatt agttaatgta ccagatgatt ttacatttgg taacgatctc cccaaaccgt
    35821 tgaagacaag ttattatgaa gcaaaaggcg atttttcttt tgatgtgcgg gatactcggc
    35881 ttccaagcac gtctccttgg caactgacag ggacattgac ttctttattt aagaatgatc
    35941 aaggtcaaga attaagcgga acaaaattat acttcaatca ttctggatca aaacaattga
    36001 ttcagcaagg tcaaaacacg ttgatttatg agagtgatgg aacagctaaa ggagaggttc
    36061 tcgttgactt tcccgatacc gatggtttac tcttagaggt aaatagcagt acgaatgctc
    36121 aacctggcgc aacgtatcaa ggcatggtca catgggaact gacagctggg ccaaccagct
    36181 aagtgattag aatttgtatg gatctgtgat aactgtcaca gatcctcttt ttcaaggatg
    36241 agaaggaaac ttctgctgtt aaaatcagaa atgcttggag gagtggactt ggaacttttc
    36301 agtttgcttg aaaaaaatga acaacggcaa atcaaacttt tccaactttt attagaggaa
    36361 aaaggagaga tttccgttaa taacgtactt acccgcttat cagttgatcg ggctaccttg
    36421 aaagaagatc tgttttattt acaagctaac ttggaaaata tcaaacaatt ggaaattgtt
    36481 atccaagaag ggaatatttt attaatcaga catggaaata tagcagcagt cgatgtttat
    36541 tatcattgct acttgcgcta ttctacaaaa tatcagatac ttctttatct gttggaaaat
    36601 ggctcatttg atcggcagaa acttgtcatg gaacttaata tcagttcggc tactttgtct
    36661 cgcagagtga aggaattaaa tgattcttta aatgagtttc atctgaaaat aaaaagcggg
    36721 cggctgaccg gtccagaaag tcaaatttgt tatttttatt tccaattatt ttggtttgga
    36781 cgtccttatc ttgaaaacaa aaaagaatac agtgatttat caggaaacac ttttttagat
    36841 ctgctgcaaa aagagtttag ttttactatt acagaagaag gacgattgaa atttgaaata
    36901 tggctttcca ttatgaagaa gcgaatgcgc tgtacgaaaa aatccaaacc agattatagc
    36961 aatcttcctg ttggattgtt tcaaacgaca caatttttat tggacctcca tacatttttg
    37021 acccgttact tctttcaata tgcctttcct tggaatcaaa atgaaacgtt ggttttttat
    37081 cttttcatga tcagtaactt tacattagat acacataact cgcatgtcca ggagtttttg
    37141 ataagcgaaa aatacaaaga cgacgtagta gctcaattga atcgaacgtt taaacaatcg
    37201 atagaagaat atttctcttt ttatgttttt ccagaagaga tttcgcaaaa gattgcgttg
    37261 acagtgatgc aaaatcacgc tcattttgcc tattttagag gatggatgaa tatttttggc
    37321 caagaaggat taaaacagcg gttaactgaa gtagaagata aacaattcct ggttttatgt
    37381 gaaagattgg tcagacaaac atttgctatt gttgatttta ctgaagtaga cgaggaaaat
    37441 gcccgctttg aattatttgc tcgctatgca tctattttgc ggacgctgat caagcagtta
    37501 gaattacgtc tgcaaattgc ttatgatctt ccttatgaaa ggctaatggc ggatatcgtg
    37561 aaagaaatca tccaggaaca attgctgaat acgatcaaat atgatttagt cgaatacgag
    37621 aaagaaaagc agtatgatat cattttgact agccaattga aagagtaccc gtcacagaag
    37681 tccgctaaag tttttgtatt cactagcaac gaatataatt atgatttccc ctatttgaac
    37741 caatttttaa aagaatgtta tttagaaaaa ttgaatttaa ggatgaataa aacatgaaaa
    37801 tcgtgatcat tggtgcttcc catgcaggca tcacagctgc cctaaatcta agaaaacttc
    37861 aaccgaaagc agaagtttta ctgatcgatg aagatcataa agatggacta ggctatgttt
    37921 caaatggaat caacctttat ttaaaaggaa agatacgtag tttagctgaa gttgcccata
    37981 acatgcggac tcttcaagaa agtggggcaa aactgatcac cgaatggaga gtaactgaac
    38041 ttgatccaga caaacatcag ctcatcctcg catccaaaga aggaaaaaac gagaccataa
    38101 cctatgataa attaatcgta gcaacaggtt cctctcctgt caccttgtat aaacaaattg
    38161 aagctgaaaa tgtctacacc tataaaaatc tagtgcaatc taagcaagta cttgccgctt
    38221 taaaagaagc aaaagaagta gtgatcttcg gtgctggtta tattggattg gaattagcag
    38281 atgctttgag aaataaagga tacattattc atctagttga ttatatgccg aatgttttat
    38341 caagatattt cgataaggat atgatcaaca gttttcaaaa tcagctgcaa actaagcaaa
    38401 tcaattttta tccaaatgaa ttcttgatcg attggaaaaa atcagaagag aaagtagtat
    38461 ccgtacaact tctatctcaa gcaataaagg cagatatggt cattttttct gcccaaactc
    38521 gtcctaatac gacattgtta aaagaaaaag tggctttgta tgaggatgag actgtgatag
    38581 tcaatgaata tctgcagaca agtgatccgg acatctatgc gattggagat atcgtcccag
    38641 tcagttttga caaaaataaa cggcatctct ttttaccttt agttactcga gctgtacata
    38701 tggcaagagc agtagcgcta actttgagtg gtcagccaac cgcttacgac ctcagacaaa
    38761 agataacagc agcagtcatt atagactact ttttaggaac tgtagggctg acagaagatg
    38821 aagcgccatt cttggaacaa aataccggtt cgtgttcagg agaatttgat ctatttccac
    38881 aatacgatga agaaaacaaa acagtaaatg caaagctgat ctaccatccg gatacattag
    38941 aaataattgg cggacagctg ataagccaag aattcttatt atcagatttg aacctgcttg
    39001 cggatatcgt gaaacacaaa actacgattc cacagctagc agttgaggat tttggatttc
    39061 tgtctgaaca cacctctcgt tttcactacc tcaatgagtt agcttttaaa gtactagcta
    39121 agagagcaaa tagcaataga gtagataaaa gactgtgaga aatatcacgg tcttttattg
    39181 tgtggggata taataaatat taatcaatcg agaataaaaa attcatataa tgaaatagcg
    39241 gaagaatttt ttgagcataa tattcgccaa aaatttatca agtgataaaa ttgttgtata
    39301 aaatgattat ttcaggagga ggagttaaat gtttttagca ttaaatgaaa ttatgcattc
    39361 aaagttacgt tatgcgttag ttgcaggggt gatgtttttg atcgcctacc tggtattttt
    39421 tttgactggg ttagcttatg gcttagctca agataatcga acagcagtcg acaagtggga
    39481 ggcagattcg attgtgcttt cgaaagatgc aaattcaaat ctagggatgt ccatgattac
    39541 taagaagata gcagaagaag tcgaaggtgg gaaagtggcg tatcttgccc agacacctgg
    39601 agtcgtgact agtaaagaca gtacagaaga aggaaaaatc aatgttagct tcttcggtat
    39661 cgataaaaat caattcatca tgcccaatct tgttgaagga aaagcttttg ataacgatga
    39721 tgaggcagta ggcgatatta gtttgaaaga agaatatggt ctagccgtag gagatacagt
    39781 taagttatct ggtagtgaca agacgtttaa actaactggc tttacggatc atgccaaatt
    39841 caatgtctca ccagttctct atacaaccat caatgcttac caagagatcc gcttcgaaaa
    39901 agaagataca agtgaaaatg cacggattaa cgcaatagtt gtcagaggta aaatcagtaa
    39961 tttacctgaa gatttagaac agatcaaaat ttctaaattc atcaatgaat tacctggata
    40021 taatgcgcaa gtattgactt ttggatttat gatcggattt ttgatcgtta ttgcggctat
    40081 tgtgatcggg attttcattt atgtactgac gatgcaaaag attaatatct ttggtgtaat
    40141 gaaagcacaa gggatcacag gaggatttat tgcgcgttct gtagtggcac aaacattcat
    40201 tttatcattt gttggtattc ttcttggtct attaggaaca gtgggaacat cccttgttct
    40261 acctgatgcg gttccattcc aaagcaattg gctgtttttt ggcgtcatta gcttgttgat
    40321 gctggtagta gctgtgttag gcgcactatt ttctgttcgg acaatcgtaa aaatcgatcc
    40381 gttgaaggca attggataag aaggaggaag agcatgaaag caatagaatt taaagaagta
    40441 gaaaaaagtt tccaagatgg cgaccaagta gttaaagcat tgaaagaaac aaccatgagt
    40501 atcgataaag gggaatttgt tgcaatcatc ggtccttctg gttcaggtaa aagtactttt
    40561 ttgactttgg caggcggatt gcagacgcct agcagtggct ctgtatttat caatgatcaa
    40621 gaattcagta atcaaaaaga aagtgaacgc gttaaactac gttttaaaga aattggcttt
    40681 attttgcaag cttccaatct ggttcctttt ctgacagtaa aaaagcagtt attattagta
    40741 gataagatca aacatgaaca tcgtcaaaaa gaagcaaaag aattatttga gcagcttggc
    40801 gtggataagt tgatcaacaa gtatccagaa gaactatctg gtggagaacg gcaaagggtc
    40861 gcaatcgctc gtgcattgta caatgatccg tccattattc tagcagatga accaactgct
    40921 agtttggatt cagaaaaggc acgagaagta gtcgatatct tggcaaaaga atcaaaagac
    40981 aaaaataaag caacgatcat ggttactcac gatacacggc tgattgacaa atgcgataga
    41041 atatttatga tggaagatgg tgtattgaaa gaaaaagaat aaccaaaaag caggtggaaa
    41101 aatggtccat cgatacaaca aagatacaca gcaaaaaatt ttgaaagaag cgactgactt
    41161 gtttatgtcc aagggatatc tcggcacttc aacaagagag atcgcacaaa aagttgggat
    41221 cacccaaccc aatctctatc attatttcgg tgataaagaa aaactatata cggcagtttt
    41281 agaaaaccac ttaaaagatg ttggaaaagc tttgagagaa atcgttcaaa caagcgaaac
    41341 gggatttcaa gtaaccttga caaaaatggc gcagtttttg attgagacac atctggtcga
    41401 tttgtttctg atgcttcacg atcttgaaag caatctttca aaggaaacaa gagatcatct
    41461 tttctttcta tggaagaaaa attatcgtga accttttgaa gaaatctttt cagcaaacca
    41521 atctgtgcta agaaatggga tttcaaaaga aatcgcagca aggcattttt tcttagtgtt
    41581 agcaccttac atcactcggt cgaaaagacc tgtggaagaa ccgctaaatg tggcgcaatt
    41641 gatcgattta tatttgaatg gtgtattagg agaagcgtaa agctgttata tagggagaaa
    41701 aatagttagg tgaagaagca agatatgacg aagatcgtta tatcttgttt tttttatata
    41761 ggtagttttt cataaataca aatttgttat tctctcatga ttatcagggt atatttttat
    41821 ttattatgtc ggtgttccag gaagtgtttt aatttaataa cgaagaaatt aattgtttgt
    41881 agaatgaagg aaactattta ttgggtagat gaatcaccac gaatagtcaa aaggagattt
    41941 caggatatgc caaaaatgaa aaaaatcatt ttagcagtaa tgttattttc tacggttata
    42001 tcttttacag cccctttttt gcgtacttaa gctgatttta agttttgacc cttagtctgg
    42061 atctacgagg tgagagtcaa gaatgaaaaa aataattgga gcttttgtat ttgcaatcat
    42121 tatcattatt atctgttttc aaatagatgt ttttggtcaa aaaaagatag ttggacaaac
    42181 ggtcagcagt acgaacagta cggttacaaa gacaaaggaa tcatttgatg atacagaaca
    42241 agagacatta atcaaaatcg gaaaaaatga ccgtacacta tatacagatg actcgctaac
    42301 taccccactt gcaactataa acggcggaga gctggcggag tttctttctg aaacaaaaaa
    42361 tgcttatcag atcaagacaa atgatggcta tactggatat ttagccctcg aagatggaac
    42421 caaagtcaca aagaatatac aaacaaaacc ggaagagcta tctgatgcag tgattgtatt
    42481 agatcctggg catggcggaa atgatactgg ggcattaagc aatgatgaac aaacagaaga
    42541 aaaagatata acattaagta cagccataaa agtaaaaaag gcgctagagg atgctggagc
    42601 aacggtttat ttgacccata ctacagatga attagtacag cttggagata tatgcgacta
    42661 cagtgaagaa aaaaaggcag atgtctttat cagtttacac gcagactcta cggaatatgc
    42721 gaatgaagca actggaatca cgacatatta ttactatgga caagaggaaa cattagcaca
    42781 gacaattgct gactccttca ctgatctgcc tctggattca agaggaatct ccactgggaa
    42841 ctatcaagtt cttcgagaaa atcttcaacc ttctatttta atcgagatgg gttatatgaa
    42901 taatgatagt gatttaaagg aattagtaac agacagttat cagcagcaga ttgctgcaag
    42961 tttgacacaa gcattgacca cttattttca gtaataaata agcccagcca aacaaaaata
    43021 gctcctcata tttttcgttt ggccgggctt attatcgcag atgcaagctt ttgaacaccg
    43081 tttaaggaag aggttgtgag cctgccgttt aactccgcgt cacaaggagc aatttcaaaa
    43141 aacagcttct catgtttgat gactattgtg acttgtgacc gaggagttgg catgagaaca
    43201 ccgtttattc ggaacaaggg actgtgacat gacttttgtc acagttccat tattttttta
    43261 gcagcttcaa agctttttct gtattggcaa aatgaagttt ggcatatttt tcaagcatag
    43321 gcatgccgcc tttctccaat agttttgccg ctacttggtc agatttcgtt ccagctcctg
    43381 atggaagggt gattccagct tcagctgatg ctttgtgtaa ctcttccaaa aagctggcac
    43441 gactgatgat agaagcagca gcaacagcta aatgatattg ttctccttta gtaacgaagt
    43501 acagtttttc ttgtaattga tttttttcta aacgcgcata ttttttaaaa tttgcttccg
    43561 gcgtaaactg atcgatcaaa atcgcttctg gtttttctgg ctcgattttt tttagcagca
    43621 aatggatcgc ttgattgtgc aaagcgactt tcatccgaac ggcattgtat tttggttgga
    43681 tctcattgta tttttctgga gataagacaa gcagtttata ggggatggtt ttttttaaga
    43741 tgtcagcaag ctggatgatt tggctatctt ttaactcttt tgaatctcta acaccaagtt
    43801 tacgtaattc tgcaagctga tcacgtttca cataagcagc acatactgtt agcggaccaa
    43861 aatagctgcc atttccgacc tcatcactac ctaaaacgga taattgactg atattttttg
    43921 gcaaagaaga ggctgttttt gctggcttgg cttgtttatc ctgtgcctcc ccccatttat
    43981 cagcttctat tgctgcattc gtaccttgaa acatcacttt tccggatgta taagcggtaa
    44041 tggtcgtccc gtcttttttt gcagcaaagc tagtataagg aatctgttta tttaacaaat
    44101 aagagcgata gtatgctttc atttgactaa gttggtcgtt tgttgctttg ataatgacgt
    44161 tgctcataaa attctcctcg tttttataca taaaactata ccgcaactca cgacttttcg
    44221 ttttctaaga gattgcgaga catagcattt ttttaaagtt tatcatataa tagttaagta
    44281 tgtgaaaaat atttgtggag aacggtgaaa gaaccaactt catggtacac taaataaaac
    44341 tttcatcaaa taggaggaaa tgattcgtat ggcgcacgaa aaaacacgct acaaagcagt
    44401 gattgccaac caaacatata ctattatcgg acgggaaacc aagcatcata tggatatcgt
    44461 aacgaagctg atcaatgaac agttagcaga attgaaacaa ttatcaccac aaatggataa
    44521 tgaacaagca gcaatcttga tggctgtcaa tgcgctttcg gatcaattga agaaacaaga
    44581 aagaatcttg gagctggaag aagaaacagc agaattaaag aaaaaaatga tcaaatttac
    44641 agagcttgaa aatcgtgtga aacgcattga agcaatcgag aacgaagctc gggaagtatt
    44701 gaaagaaaat ggtcaagcag atcataccat taaaaatcat gtggaagcac aacagatttt
    44761 aaacgagaaa cgaaaaggac aaattaaaca aaaatcttct aattaagatc cttttatcaa
    44821 aataagaaaa atttaaaaaa gttagaagag aaaggaacgt agcatgctga gtttgctgat
    44881 tttattttta ttgctaattg cgtttttttc tggagcacgt cgcgggtttg ctttgcaaat
    44941 catattcgcg attggatatg tattgtcatt tattgctgcc cagcattttt acaaaccatt
    45001 agcaagtcat ttggaattat acattccata cccagctgtc accccaacat caaagttagc
    45061 attttttgat caggtatttg cttttcacct agatgaagct ttttatgcag gaactgcttt
    45121 tctgattatt ttgctaattg gctggctgct tacccgattt gtgggggttt ttgtccatgg
    45181 attgacgtat gtgccgatct tgagacaggt agactggatt gcaggaggca tcctgtcact
    45241 aatcatggct tacgttacta tatttttgat tttgcagtta cttgcctttg ttccattaga
    45301 tgtggtccaa aatcaattcg gcgagcatag tttcgctcgt tttatcgttg agagaacgcc
    45361 atttcttacc aacaagatgt atgagctttg ggtaacgaat attttgaatt aaggaaaata
    45421 gtgctggaaa atatttcgtt ttttgtacag agtagtttat ttaagaaggc tgcgtagcgt
    45481 atgtccggca cagctctttt ttcatagtga aagataaggt gataaaatga ataaccgaat
    45541 ccttgaaaca ttggaatttg aaaaagtcaa acagatggtc agacaatttg tagtgactgc
    45601 acaaggaaaa gaagaactgg cagaattggt accagtgagt gagaaacaaa cgatcacaaa
    45661 ctggcttcaa gaaactgagg atggattgaa agtgcaacgt ctgcgaggcg ggatccctat
    45721 acctaaactt gaaaatatcc gtccccatat gaaacggatc gagatcggag cagacttgaa
    45781 tggagtggag cttgcacaag tttccagagt attgtctacg acaagcgaat tgaatcgttt
    45841 cattgatgat ctttctgaca gtgaaatcga atttgcacgt ttgtatatgt gggcagatca
    45901 gttagtcacg atccctgttt tgagccgccg tttaaaggaa gcaatcgatg aagacggacg
    45961 cgtgactgat gacgcttctc ctgagttaaa gagcataaga cagaacattc ggcgaagtga
    46021 acaagctgtt cgtgaacagt tagatgggat cgtccgtggg aaaaatgcaa agtatttaag
    46081 tgatgcgatc atcaccatgc gaaatgaccg ctacgttatc ccagtcaaac aagaataccg
    46141 cggtgtattt ggcggtgtag tccacgatca aagtgcttca ggacagactt tatttattga
    46201 accaaaacaa gtggtggatt tgaataatcg tttgcgtcaa taccaaatcg ctgaacgaaa
    46261 tgagattcaa cgtattttaa gtgaactttc tgctgaactt gttccccatc gtcaagaaat
    46321 catccacaat gcttatgtga ttggtaaaat ggatctgatg aatgcaaaag cacgatttgg
    46381 taaagaagta aaagccatcg tgccgggaat cagtgaagac aatcatgtcg tcttgaaaca
    46441 agcaagacat ccactgatcg atcaggaaaa agtagtttct aatgacatca caatcggaaa
    46501 agactatcaa gccatcgtca ttacaggacc taacacaggt gggaaaacga tcacgttgaa
    46561 gactttaggt ctgttacaac tgatggggca agcaggatta ccgattcctg caggtgaaga
    46621 aagtcaaatc ggtatttttg aggaagtatt tgcggatata ggagacgaac agtcgattga
    46681 gcaaagtcta tcgacctttt cttctcatat gacgaacact gtagatattt tatcaaaagt
    46741 caatgaaaaa agtctggtat tatttgacga gctcggtgca ggaacagatc cgcaagaagg
    46801 tgctgctcta gcaatcgcaa tcttagatga tcttggtaaa aagtctgctt atgtgatggc
    46861 aacaacgcat tatcctgaat tgaaagttta tggatacaat cgggcaaata cgattaatgc
    46921 cagcatggag ttcgatgtgg atacactaag tccaacgtat cgtttgctga ttggcgttcc
    46981 tggacgaagc aatgcgtttg aaatctctag ccgattaggt cttgatacag aagttattga
    47041 tgaagcgaag cagttgatga atgatgagag ccaagattta aacgaaatga tcacagattt
    47101 agagaatcgc cggaaaatgg cagagaccga atatctggaa atgcgtcatt ttgtttctga
    47161 ggcacaagaa cttcacgatg atttgaaaga agcttacagc tatttctttg aagaacgtga
    47221 aaaagaaatg gaaaaagcaa agaaaaaagc aaacgaagtc gtgtcagagg cggaagaaaa
    47281 agcagaaaaa atcattgctg atattcgtaa aatgcaacag cagattgggc aaggaaatgt
    47341 gaaagagcat cagttgatcg atgcaaagac ccaattagca aatctacatc aagaagaaac
    47401 tttgaagaaa aataaagtat tgaagaaggc gaaagaacaa aaaacgttga aaccgggaga
    47461 cgaagtgttg gttacgactt acggccaaag agggacgctt ttacgtaaaa acgggaatca
    47521 gtggcaagta gaaatcggta tattaaaaat gaatgtgtcc gaagacgagc tgacgcctgt
    47581 tgctcctcaa aaagagccga cacaacgagt tatccatgct gtacgatctg aatctagtag
    47641 tcacgtgccg aatcaattgg accttcgagg aaaacgatac gaagaagcgc taagtgaggt
    47701 agatcaatat ttggattctg ccattctagc tggttatcct caagtgacta ttgtccacgg
    47761 aaaaggaaca ggcgcacttc gcaagggaat tactgattat cttaaaaacc atcggagcgt
    47821 caaaagcttt gagtttgcac cggcaaacca aggcggaaat ggtgcgacaa ttgttaaatt
    47881 taagtaaagc ttactaaagg taagcagatg gttcgctatc caagtataga aatgctatac
    47941 taatcataga ttttaaaaat ggaggtcttt gatatgtcac aagtaattac agataagaca
    48001 tttaatgaag aaacagataa aggacttgta ttgattgatt tttgggcaac ttggtgtggt
    48061 ccatgtcgta tgcaagcacc gattttggat cagttggaac aagaatatga tgaagaagaa
    48121 ttcagaattg ctaaaatgga tgtggatgaa aatccagaaa ctcctcaaca attcggtatc
    48181 atgagcattc caacattaat gttgaaaaaa gatggtcaag tagtagaaaa agctgttggt
    48241 gttcattcaa aagaacaact acgtcaaatg atcgatcaat atttataaaa acttacagca
    48301 aaaataagct gagggtggga ctgaatccag ttccgtcctc ggtttttttg cggttgagtt
    48361 aaggttattc aaattaatat tttttgagta taattacgtt gtcaggctgg attgtttttt
    48421 tgacagacat gctataataa ctttaattga atatgaaggg gagtaccaga tgaacgaacg
    48481 aatcaaaaac aaattagctt tacttcctga tcagcccggc tgttacttga tgaaagataa
    48541 aaatggaacg atcatttatg tcggcaaagc caaaatatta aaaaatcgtg tccgctcata
    48601 ttttactggc agccataata cgaaaacgga acggcttgtt agtgaaatcg aagactttga
    48661 atacatcgtt acagaatcga atatcgaagc attgctctta gagatcaatc tgataaagaa
    48721 aaatgatcct aaatacaaca ttatgttaaa ggatgataaa acctatcctt tccttaagat
    48781 aacgaatgaa aaatatcccc gtttggtgat tactcgtaaa gtactaaaag acaaagcact
    48841 gtattttgga ccatatcccg atgtcggtgc ggcgaatgag acaaagaaga tcctcgatcg
    48901 attatttcct ctgagaaaat gcaaacctag tcagacgaag gagccttgtc tttactatca
    48961 tttgggacaa tgtctgtgtc cttattattt cgacgtagat ccgaaagtct atacgaaaat
    49021 cgttgatgaa gtgaaaaagt ttttaaacgg aggacacgaa aaaatcgagt cagagatcca
    49081 tcagaaaatg gaaaaagctg ctgaaaacat ggagtttgaa aaagctgcag aataccgaga
    49141 ccagatccgt gccattgaga caatcatgac tcgccaaaaa atgacaaata ctgatttact
    49201 ggatcgagat gtatttggtt atgcgattga caaaggatgg atgtgtgtcc aagtgttttt
    49261 cgtaagacaa ggaaagctaa ttgaacgaga tgtttcaatg tttcctttct ataacgaagc
    49321 agaagaagat ttcttgactt acctcggtca gttttatcag gaaaatgagc atttcattcc
    49381 taaagaagtc ttgattcctg atacgatcga caagcccagt gtagaagctt tgttagctac
    49441 gaaagtgctg caaccaaaac gaggagaaaa gaaaaaatta gttgaattgg cgaataagaa
    49501 tgcctcagtt gctttgaatg aacggtttga tctgattgcc agaaaacagg aacggacgat
    49561 cggagcagtt gaacgtttag gaaacgcgat gaatatcccg accccaatac gtatcgagtc
    49621 ttttgataac tctaatatca tggggacgaa tcctgtggct gcgatggtcg tattcattga
    49681 tggaaaaccg gcaagaaaag aatatcggaa atataaaata aaaacagtca acggaccgga
    49741 tgattatgca tcaatgagag aagtcatcta tcgcagatat tcacgagtga ttcgcgaagg
    49801 gttgccttta cctgatttga tcttgatcga tggaggaaaa ggacaagtag atgtggcaaa
    49861 agatgtctta gaaaatcaat taggaatcga tattcctatt gctggcatgg caaaaaacga
    49921 taaacataaa acaaatgagt tgctattcgg tatagatttg gctgtagtac cgcttgagcg
    49981 aaattcacca gagtttttct tactccagcg aatccaagat gaagtccatc gattcgcaat
    50041 cactttccat cggcagctac gcagtaaaaa cagttttgct tcccgattag atgaaattga
    50101 agggttagga ccaaaacgaa aaaaaatgct gcttaaagag ttcaaatcgt tgaaaaatat
    50161 cacagcagcc acgacagaag aattacaaga aatcggactg ccgaaaaatg tcgcacaaaa
    50221 cgtatttgat aaattacatc aggaatgagg aaagggcact ttacaaagaa agtaaagtgt
    50281 cctatcattt tatgtacaac aaaaagagcg tttacgtttt gttggtaaat aataaatcgc
    50341 ttacgtctgg tgtctaagga tatatgtgat ttcttgaaga aggagtgaag aaatgttaga
    50401 agtcaaggat cttgttaaaa cttttgggaa ctacacagct gtcgatcatg tctctttcca
    50461 aatccccgat ggaaaaatca tggggctaat tggccaaaac ggggctggga agacaacgac
    50521 ttttcgtttg atcttggatt ttttgacaca agaccaagga gaagtgttat ggaatggcca
    50581 tcatcttggg gagaaagatt atgatgtcat cggttatctt ccggaggaac ggggacttta
    50641 tccgaaagta acgattcaag accaactgct ttattttgca gaacttagag ggaaatcaag
    50701 aaaagagatt gaaccaaaaa tcgatttttg gatggaaaaa tttcaggtaa aagggaaaaa
    50761 gacggataaa gtaaaatctt tgtctaaagg aaatcagcaa aaagtccagc tgatcgccac
    50821 gttaatccat gaacctaaat tgatcatttt ggatgagcca ttcagcgggt tagatccggt
    50881 aaatgctgaa ttgctaaaag atggaatatt ggaattgaaa aaacatggtt catgtgtcat
    50941 tttttctagc cacaatatgg ataatgtaga aaaaatctgc gatcatttga tcatgttgcg
    51001 aaacggaaaa atggttttga atggaaaagt acatgagatt cgtgaatcat ttggccgtac
    51061 gaagttattc ttggaatctg gtttaagcca acaggaagta gaagaaatcg aaggcgtgaa
    51121 aaaagtgatc cttcatgaag acaatacgtt agagattacc ttagaagatc ctgaagctgg
    51181 gaaagaaatc tttactcgtg cgactcagtt cggctacatt cctatgttta accagcaacc
    51241 gccaactttg gaagagattt tcaaattgaa agcaggtgaa atcgatgcgt aagttttgga
    51301 ttattaccaa agatgtctat ttaaaaaatg ttaagtcgat ttctttcttg atcatgatat
    51361 tagtcccttt tatcatgctg ggtatcattt atgtagcagg aaactttgct caacaaaata
    51421 gtgatgtcaa taaaatcggc gtattggctg ataatgagca aatcagtcag caaatgagcc
    51481 aagtcaaaac agatgatttt gagttcaaag cagttgattc tgaagaaaaa gcacaaaaag
    51541 agctttcgga agaaaaaatc gatgcgtatt tagtagtctc aactgagaat gataagataa
    51601 atgggatgct ttatagcgaa aattcattag gacaaagtac acaactactg atacaacaac
    51661 aactgactgg cttgcaatcg atgcttcgag caagtcaatt aggcatttca ccagaagaag
    51721 tagctagctt gagccaacaa gcaagtttga ctcgacaaaa agtcaatttt gattcaaaag
    51781 gaaaaatgac agttggagaa gataactcga cagtccaata tgtcgtaagt tatgtagcaa
    51841 cgattgtctt gttcattatt attttgacgt atgcgcagat cattgcacaa gagatcgctt
    51901 ctgaaaaagg cacgcggatc atggaagtga tcctgtccag tacgactgca caaaagcatt
    51961 tttacggaaa actaacaggt gttttacttg ttgcattaac gcaaatggca ttatacggta
    52021 ttatttttgg tataggatac agtcaattca aagatatgga aatggtcaaa aatatattag
    52081 acggtatctc attggataac ttgattggac cattcttgtg gtattctctt ctattcatga
    52141 tctttggcat tttgattttt tctgttttag cagctttatg tggctctttg gtcaacaaag
    52201 cagaagatac agcaaaagca attttgccag tgacttatct atctctaggc ggatatatgc
    52261 ttggattgat actaggagct tcagatccaa acaatatcgt tattcggatc acttcgtata
    52321 ttccattctt atcttcatat attatgccgg ttcgcttagc aaatgagaca gtcggtatgg
    52381 gtggagcatt gatttccttg ttggtacttg tagccgtaac attcttattg atgatcttgt
    52441 ctgcaaacat gtacaaatcg aatgtactcg tgtatagcga aggcggatta tggagttcat
    52501 tgaaacaatc gatttcaatt atgagaaatg aacgaaaaaa agcataaaat atagaaaaat
    52561 atagaaggaa gctggacatt tattgttccc agcttctttt ttgtatagac ctatttctat
    52621 ttttttatta tattggatat atatattcat tgaaaataga gcataacaac tttataatgt
    52681 tgagtatacc aatttttgga ggcgttttaa tggaccgcta tttagattta ctttcagaga
    52741 agttccctac aacggaagat gtcatcacgg aaatcatcaa tctcgaagca attttgcagc
    52801 ttccaaaagg aacagagtta tttttgagcg atatacatgg cgaatttcct gcttttgatc
    52861 atattctgcg tatcggttca ggaaatctta aagaaaagat caaagacctt ttctctgaac
    52921 aaatgacgga aacggagatc agccaattca ctatttttac ggcttatcca gaatatgtgt
    52981 tgacgactga ttggtacaaa aaacaagata aatcccagct gatccatcgt ttgatcgatc
    53041 ttttacggtt tacgacagtc aaatacactc gatcaaaagt tcgaaaagga ttgccgaaag
    53101 aatatagcta tatcattgag gaactcttgt atatcgatga tcgtatcaac gggaaaaaag
    53161 actatgtaaa aaaaatcatt gaacaacttg tcttgatgaa ggaagaagaa aaatttttaa
    53221 caaaacttgc gcagacgatc caaaaatctg tcatcgatca tttgcatatc gtgggtgata
    53281 tttttgatcg aggaactcaa gcagccaaag taatggatcg aatcatggat ttttcctctg
    53341 tagatatcca gtgggggaat catgatattt tgtggattgg tgcgtattgc ggctctgagg
    53401 catgtttatt gacgctttta cggattgccg cacgctacaa ctatttattt gaattagaga
    53461 aagaatatgg attaaattta cgtccattat tttcttttgc ccatcaaacc tatcagaaga
    53521 atccagtatt tacccctaaa aacaggaaag atctatctga aagagagcaa gaagaattag
    53581 aaaagatcca tcaagcactt tctatcctcc aattcaaatc agaacatcag ttgttgaatc
    53641 gacgacctga atttaaaatg gacgatcgta aattgttgga aaaaatcaac tatgaggaat
    53701 caacgatcgt tttagaaggt cagctctatg agttgaaagg gacttgtttt caaacgatcc
    53761 agccagatga tcctaaaaaa ttcttggaag aggaacaaaa agtgatcgat agtttgatgt
    53821 attcattcca gcactcgtta agaatgcaaa aacacatgac gtttttgatc gaaaaagggg
    53881 gaatgtatct cacgaacaat cagcatgtac tgttccatgg ctgtatacct gtagatgaaa
    53941 aggggcagct cttagcattt gaattagacc aatcttatcg cggaaaagaa ctgctgggat
    54001 tttttgaaaa acagataact gaaagcgcaa aagatttgac tgcaaatgaa gatttggcga
    54061 ccgatttgat ttggtatgcg tggagcgggc cattttcgcc tttattcggc aagagtaaaa
    54121 tggcaacttt tgagcgctat tttctcacag agcctcatac gcacgtcgaa aaaagcaatc
    54181 cctattatca cttgcgagat gaagagtggt tcagtgaaaa aattttggca gaattcgatg
    54241 caaaagaaga aggttcagca atcatcaatg gacacacgcc agtaaaagta aaaaaaggag
    54301 agtcaccgat caaagcaaac ggaaaagcat ttgttattga cggaggtcta tccaaagctt
    54361 atcaaaaaac aaccgggatt gctggctact cattactatg caattcttac ggtttccaaa
    54421 tcgtgacaca ttatccgttc ttgcctattg aacaacaatt tgcaaaaaaa tctgatcaaa
    54481 cgaatgtaaa aaaagtgatc gagcagcagc tcccacgcaa acttaatcgg aatacgacaa
    54541 aaggaatgga attacagcaa cagattactc aactccaaag gctgttggat tatcgaaagg
    54601 acgattgaga aaaatgctag ccaacattgc tcaatagact aaaaagcgac tgcaacaaaa
    54661 ggcgtgtcgc agtctccttc ggaattagct caaaaaacgg taaaatatta gaagctattt
    54721 ttctgtttat ttctctaata ctcaggtcag ggcgctgatt tcacgacctc ttccttaaac
    54781 ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccaacaaaca tgagaagctg
    54841 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctaatcagaa
    54901 aaacgattaa aattcattca caaagaaatc gaataaacgc tgttcagatg aatctacacg
    54961 atgagaaagt tccggatgcc attggacacc aaggattttg tgcatattat ctttactttg
    55021 gactgcttca atgagtccat cgttggaaaa ggcgatggct tttaacaaag gagaaagatc
    55081 tttgactgcc tgatgatgat aggaattcac aaagtatgaa tcagggagaa ggtcacttaa
    55141 taaactgtcc gaaacgattt tgacttcgtg agttgcaaac tgcggggcag tgggctgttg
    55201 ttcgtgtttt acggaccatt ttggatataa tgaaagatct tgataaagtg ttccctccaa
    55261 cacgacattc aatagttgaa gtcctcggca gactgcaaaa atcggttttc tttgtttcaa
    55321 tgcttccaag atcaatgcct gttcgaattg gtctcgttga atatttgttt ccgtcagttt
    55381 tggatgaggg tcttccatgt aaagttgcgg actaacatcc tgaccgcctg caagaatgag
    55441 tttgtcgatt ttctcaatta gttgaggcgc tgaatctgga gacatgactg gcagaataag
    55501 aggtaatcct tgagcatcta aaactgcatc cacaaatccc tgcggtgtat agcttacttg
    55561 atttccttgg aaagtatctg tcgctcgaat cagttgattc ccggcaatgc cgatgattgg
    55621 tttcattttt attcccccta aagtagacta attgtaacaa ggatttaaaa aaatttaaac
    55681 tatgtaagac ttgcccctat acttatctgt tcttgctgtg ttactatctg taaagagaat
    55741 tttcgtgtga aattcatgac gatagttgaa aatattgctt aatttgttat aataaaaagt
    55801 aagcagaaat aaaaaggttg ggcgtaagct tcaaggatct agttcttcaa ggggtgagaa
    55861 ggacatttta ttgaaggggc ctgcctatac taagttatct ttagaggtac atactttcaa
    55921 tgaaaaaaga aagcatgtac ctctttttta aatcgaaagg aagtgaagca agtgttagaa
    55981 gctttggaac aaatccgaaa agcagaagaa gaaaatgaga agcgtgctcg aaccctacgg
    56041 cagcagttga agacctatgc agaagaaaaa gagcaagaat tggagataat ccaaacgaac
    56101 agtcggcaac agctgaaaca gttggtagaa gaaaaagaac aattcgaaaa gcaagaatta
    56161 gaaaaagaaa aaatgaaatt gaaagcagaa gcagacaagg tgcaagcaac tttgaaagac
    56221 caatatcaaa agcatcagag tcaagcaatc ggtgcaatca ttgaaagggt gaaagaaact
    56281 tatggccgtc actaagatgg aaaaagtgac cctcatttca gataaaagaa atcaggagag
    56341 gattttacaa gcagtacaag gaatccaaaa tgttgaaatc cgggatctgt atcaaaatag
    56401 aacaaacaac cagtgggtag aaaaatattt tccagatacc acgattattg ataaagaagc
    56461 aaaattaagc gctttaggca atcggctgaa agacatccaa gaagctatcc aattcataca
    56521 acaccacggc gatagccgtc aaaagaaact ccatttgaaa catcaagaac tttccttaca
    56581 agacttggaa gcaaattatt ccgaagaaaa tttcatgaat aaattagaag aagtccttga
    56641 tttaaaagaa aagtgggaac agctaactga aaaacgtgat cgtttagaag aagaggaaga
    56701 atggcttctt cattggcaac agctggatat tgctccaagt atgtatgcaa gtaagacgac
    56761 catttttgag atggggactg tgagtgcagc aaattttgaa agtttcaagg aagaactaag
    56821 taagcttgat gacgtttata tggaagaaat agattatacg ccaaaacaag tttatctttc
    56881 gtatgttgct ttaaaaaaat cagttgctgc tatagaagaa attgctggga aatacgggtt
    56941 caataaagaa acgtatcctt atgaacaaaa tcctaaagaa aaattgcaag aaaccaaaca
    57001 gcaattatcc attgttcacg agcagcaaaa aaagataacg acagcgttag gtcagtgcag
    57061 cggctatatc caagaatttg aatgggtaga agaagtcaca cttgccgttg cagaacggga
    57121 aaaagtaaaa gaacggttta tccatgccac ttatctgatc gttttgcaag gttgggtaga
    57181 tgcagaagaa aaacagaatt tgatgcatgt gttaaacgaa actgtatcag aagaagatat
    57241 atacgttagt ttcgaaacac caaactcaga agagattcag caggaagtcc ctactaaatt
    57301 gaaaaaccat ccgcttatcg aaccttttga attgctgact gaaatgtaca gtctgccaaa
    57361 atatgaagaa gtagatccaa cgccttggat gacaccattt tatcttgtct tcttcgggat
    57421 gatggtcgca gatgttggtt acggattgct gatgctcatt gggacaatac tagctcaaaa
    57481 actatttgtt ttaccccgcg ggatgaaacg tttcgtaaaa ttctttgaga ttctctcggt
    57541 accatcgatc atctggggat tgatctacag ttcatttttc ggacaggcat taccaaaaga
    57601 actatttggt atccgcttgc ctttcccaat cctatctacg acggatgacg tcaatacgat
    57661 tttgatttta tccgtgattt tcggtctgat acagatcttg gttggattat tcgtggcagc
    57721 aaaggaaaat atcaggagga aagattactt gagtgctgtc agtgatggtt ttgcctggca
    57781 agggatcctg attgggatcg ttattgcatt gattggtgtc atgttgataa aaaatacagc
    57841 atttgtttat cttggcgcgg ggctatcgat cattgctgct ttatgtatct taatcgtgcc
    57901 gattatccaa tcgccgtcta aagtcaaggg tgctgccaaa ggagcgtata atctttacgg
    57961 attgactggt tatatcggtg atttagtcag ttatacacgt ttgatggcac taggtatttc
    58021 aggcggaagt attggcgcgg cattcaatat gctagttgct ttcatgccgc cagccgctcg
    58081 attcagtgta gggatccttt tgatcatcgc tttacacgca ctgaatatgt ttttgacact
    58141 gttaagtgcc tatgtccatg gtgcacgatt gcagtatgtc gaattttttg gaaaatttta
    58201 tacaggaggc ggacgtgcct ttgacccgct gaaaacggcg gaaaaatacg tcaacatcaa
    58261 tcataagaaa aaaaacaata aataattgga ggatttttat acaatgatgg attacttaat
    58321 tagtcaaaat ggtggaatgg tatttgcagt attagcgatg gcaacagcga cgattttttc
    58381 agggattggt tcagctaaag gtgttggaat gacaggtgag gcagcagcag cgttaacaac
    58441 aagtcaaccc gaaaaattcg gacaggcttt gattctgcaa ttgcttccag gtacgcaagg
    58501 gttgtatgga ttcgttatcg cctttttgat ttttatcaac ttaggaaatg atatgtcagt
    58561 tgtacaagga ttgaactttc taggcgcttc tctgccgatt gcttttacag gtttgttttc
    58621 aggtatcgcc caagggaaag ttgcagcagc aggtatccaa attttagcga aaaaacctga
    58681 acatgctaca aaaggaatca tttttgctgc gatggttgaa acatatgcga ttttaggttt
    58741 cgttatttcc ttcctattag tattgaatgc ctaagcataa aggaggaaaa gcagatgaac
    58801 gccattgaaa aaatcatctc tcagatgaat gaagcagcag agcaagaaag agcggcgtta
    58861 gagcaagaag agcgaatgaa gatcgatcaa aactttgaac aaaaacgtac gcaaatcgaa
    58921 acagaacatc aaaaacaaaa agaaaaacaa atcgaattac ttgaaaaaaa ataccgtcag
    58981 cttaggaatc gccaacaagt agaagtacga caagaaaatt tgaatgcgaa gcaggaattt
    59041 ttacgacgat tgtttgcaga tgcaatagct gaaatggaga attgggatga atctgaacaa
    59101 atccagttta tcaaaaattc attgtattca ttacctttga caggcaaagt aacattcatt
    59161 gctggagaaa aatcagcagc ttatctttct cagacattgc tggatgaatg gaacaatgaa
    59221 cttcccttta tgatggtttt aagtgatgaa actgttgctg atcaagcggg ttttctgata
    59281 aacgatcaag gagttcagta taattttctt ttcagcagcc ttgtacaaga tatccaagga
    59341 acgatgagtt ttgagattgc caatcaatta ttcgaataag gagggatcag tgtatggaat
    59401 accacgaact aaatcccttg attcgcggaa gagagcttga acttttgtcc aatgagacat
    59461 ttgaacggat gattcaaaca aattctgtcg aagcacttgg tgaaatattg aaatccacga
    59521 tttacagtcc atatatttat gacggatatg aatatgactt cgaacagaat cttttaaagg
    59581 agcagagccg tctatttcgc tggttaaagg aatcagcacc ggagcctgaa atcgtttgga
    59641 tctatacgat gcgctatact ttccataatc tgaaggtttt gacaaaagcc gagatgacaa
    59701 atcaaaatct ggaccatttg tatatcgacg atggatttta ttcattagaa accttgaagg
    59761 atgccattca tacgcaagtt tcaaatgaac tccctgaaag cattatggac agtatacgag
    59821 aagtacgtga ttattttgaa gagtctacca ttcttcaggg aattgacgtg atttatgatc
    59881 gacagttttt gacagagcag cgacggcttg ccgataagct aggatatcaa gaattgctga
    59941 cagaagtcat tgcttttatt gacttgacca atatcacaac gatggctcga ggaatattgc
    60001 agaaaagaac atatgggttc atgacgacgg tactttccag ctcgggaagt atcgaaaaag
    60061 aagtattttt gaactttgta gataaagata tggaagatta cacgaatttt ctattagaga
    60121 cagattatgc tgattttctg cgccctgtca tccgtgaggg aaatcttgat ctcgtaaagc
    60181 ttgaacagtt gaaagatgat tatctaagtt cattgtatca agaagcacaa acccaggctt
    60241 tcggaccact gccactacta gcctttttga acgcaaaaga ggtagaaagc aaaaaccttc
    60301 gtttactggt tatcggcaaa agaagccatt tcgaaccaga aacaatcaga aagcgggtga
    60361 gacaagtcta tgacgcataa gatcggtgta gttggcgaca aagattctgt tttgccattc
    60421 aaattgtttg gctttgatgt tcgttatggt acaaccaagc aaaccgttcg tcaggcaatt
    60481 gaagaaatgg caaagaatga gtatggcgtg atttatgtca cagagcaatg tgcagcattg
    60541 agcccagaaa cgatcgaacg ttacaaggaa caaatgatac ctgctatcgt attgattccc
    60601 aatcatcaag gatcattagg gatagggctg gaagaaattc aaaaaaacgt agaaaaagca
    60661 gtaggtcaaa atatcttata gtatgaaaag gagatagaaa ttttgcaaat tggaaaaatc
    60721 ataaaagtct ccggacctct tgtaatggct gagaacatgt ctgatgccag tatccaagac
    60781 atgtgtctag tgggagatct aggagttatc ggagaaatta tcgagatgcg tggcgatgtg
    60841 gcgtctattc aagtatatga agagacttct ggaattggtc caggcgaacc ggttcgctct
    60901 actggtgaag ctttatctgt tgaattagga ccagggatca tttcacaaat gtttgacgga
    60961 atccaacggc cattagatac ctttatggaa atcacacaaa gtaatttctt agggcgcgga
    61021 gttcaattgc ccgcattgga ccatgagaaa aaatggtggt tcgaaccaac agtagaagcc
    61081 ggagaaacgg tgagtgctgg agatgttatc gggatcgtag aagaaacaaa agttataaaa
    61141 cataaaatca tggtgccaaa tgggataaaa ggaacgatca aaacaatcga aaaaggttca
    61201 ttcactattg atgaaacagt atgtgtcatt gaaaccgaaa atggtgacaa aaatttgacg
    61261 atgcttcaaa aatggccagt aagacgtagt cggccaatca aagaaaaatt aaacccggat
    61321 gcacccatga tcactggaca gcgagtgatt gatacattct tcccagtgac aaaaggagga
    61381 gcagcagctg tacctggtcc ttttggtgca gggaaaacag tcgttcagca tcaaattgca
    61441 aaatgggccg atgtggacat ggttgtctat gtgggatgtg gcgagcgtgg aaatgagatg
    61501 acggatgttt taaatgaatt ccctgaattg attgacccta atacaggaga atcgttgatg
    61561 gaacgtacgg tattgattgc taatacatca aacatgccag tggcagctcg agaagcttct
    61621 atctatacgg gaatcacaat tgcggaatat ttccgtgaca tgggttatga cgttgctatc
    61681 atggcagact ccacttcacg atgggcagaa gcacttcgtg agatgagcgg acgtttggaa
    61741 gaaatgcctg gtgatgaagg ttatccagct tatcttggtt cacggcttgc ggaatactat
    61801 gaacgatctg gaagagtcat tgcattagga tcagaaggtc gcgaaggaag tataaccgca
    61861 atcagtgctg tatccccgtc tggaggggat atttcggaac cagtcacaca aaatacattg
    61921 cgggtcgtaa aagtattctg gggtcttgat tccagtttag cccaaaaaag acattttcct
    61981 tcgatcgatt ggatacaaag ctattctctt tattcaacag aagtagggaa gtatatggat
    62041 catatccttc aagctgactg ggcatccatg gtcaccgagg gcatgcgtat tttgcaggaa
    62101 gaacagcagc tgaatgaaat cgtgcgtttg gtagggattg attccctttc agataatgac
    62161 cgtctgacat tagaagtggc aaaatccatt cgtgaagatt atttgcagca aaatgctttt
    62221 gatgatgtcg atacattcac ttcaagagag aaacaattca aaatgttgaa gatgatcttg
    62281 acctttggtg aagaaggacg ccgtgcgttg tcgttaggtg cttatttaag tgagatcatg
    62341 gacggtaccg tatcgattcg tgaaaggatc ggacgaagca aatatatccc ggaaacagaa
    62401 ttagaaaaac tagatcaaat tattggtgaa atcaagcaaa cgatgcaaac gatcgtttca
    62461 gaaggaggca tgagcaatga ttaaagaata tcgtacgatc ggtgaagtcg tcggtccatt
    62521 gatggcggtt gaaaaagttg ctggtgtcaa atatgaagaa ttaatcgaag tccgtatgca
    62581 aaatggcgag attcgtcgtg gacaagtatt ggaagtccaa gaagataaag caatggtcca
    62641 aatattcgaa ggaacaagtg gtatcaattt acgtgactct tctgtacgat ttttaggcca
    62701 tcctttagaa ctaggtgtat cggaagacat gatcggtcga gtattcgatg gtttgggacg
    62761 accgaaagat aacggaccag agatcctgcc tgaaaagcat ttggatatta acggagaagt
    62821 aatcaatcca gtagctcggg attatccaga tgagtttatc caaacaggta tctctgcaat
    62881 cgatcatttg aatacattag ttcgtgggca aaaattacca gtattttctg gctctggtct
    62941 tcctcataaa gaattagctg ctcagatcgc gcgacaagca acggtactcg attcatcaga
    63001 tgatttcgcc gtagtctttg ctgctattgg aatcactttt gaagaagctg aatttttcat
    63061 ggaagatttt cgtcaaacgg gcgcaatcga ccgttctgtc atgtttatga atttagcaaa
    63121 cgaccctgca atcgaacgta tcgctactcc tcgtatggct ttgactgctg cagaatatct
    63181 tgcttatgaa aagggaatgc atgttttggt tatcatgaca gacatgacca actatgcaga
    63241 ggcattgcga gagatttctg cagcacggcg agaagttcca ggaagaagag gatatcctgg
    63301 gtatctttac acgaacttag ctactttatt cgaacgagca ggaagaatcc gcggactaaa
    63361 aggatcggtt actcaaattc cgattctaac aatgccagaa gacgataaaa ctcatccaat
    63421 ccctgacttg actggttata ttacagaagg acagatcatc ttgactagag agctttacaa
    63481 gagtgggatt cagccaccaa ttgatgtact cccgtctctt tcgcggttaa aagataaggg
    63541 aactggaccc ggaaagacaa gagaagacca tgcagctacg atgaatcagc tttttgcagc
    63601 ctatgcacaa ggaaaacagg caaaagaatt agccgtagtt ttaggtgaat ctgctttatc
    63661 tgatgtagac aagatctatg cgaagtttgc tgagcggttt gaaaaagaat atgtcaatca
    63721 aggattctac acgaaccgtt cgattactga aacacttgat ctagggtggg aactgctatc
    63781 gatgttgcca agaaccgaat taaaacgaat caaagacgac atgctggaca aatatctgcc
    63841 agaagggaag tgagttctga tggcacgatt gaatgtgaat ccgacccgca tggaattgac
    63901 tcgattgaaa aaacagttga caactgccac aagagggcat aaattattaa aagataagca
    63961 agatgaactg atgcgtcaat tcatcctgtt gattcgaaag aacaaccagc tgcgtcaaga
    64021 aatggaagag tctatgacag aagcaatgtc tgattttgta ttggcaaatg cttcgatcac
    64081 ggaacctttc atcgaagaat tatttgtttt gccggcggaa aacgttgatt tgtctatcgt
    64141 agacaaaaat atcatgagtg taaaagtacc tgtgatgaat tttgaatatg atgaacagct
    64201 gacagacaca cctttggaat acgggtattt gaattccagt gccgaactgg atcggtcaat
    64261 cgatcggttt actaccctgc ttccaaaact tttggaactg acagaaatcg aaaaaacttg
    64321 tcaactgatg gcgagtgaaa tcgaaaagac acgtcggcga gtcaatgcat tagaatatat
    64381 gacgatccct caattagaag aaacgatcta ttatattcgt atgaagctgg aagaaaacga
    64441 gcgcgcagaa gtaacaaggt tgatcaaagt aaaaaatatg ggtaccactg actaagtggt
    64501 atcttttttt aaaagaggaa aacgatgatt aaacaaagca aacaaataaa gaaacgtttg
    64561 ccggctgttc agcttctcgc attaggattt tttctcctga ttttagttgg cggcggccta
    64621 cttaccctac cattttttag taatagtggg catgggacaa attttatcga tgcgcttttc
    64681 actgcaactt ctgctgtctg tgtaacggga ttgacaacat tgaatacagc agagcattgg
    64741 aacagtgctg gtcaattttt gatcatgctt ttgattgaga tcggcggtct tggttttatg
    64801 atggttccga ttctatgctt tgcattgata aagaagaaag tcagtctcag cacacgtatt
    64861 atattaaaag aagcgctgaa tctagaagaa gtatcaggtg tgatgaagct catgctttac
    64921 atcctaaaat tggcctttgt tatccaattg attggtacgg ttgctttatc ttttgtattt
    64981 gtgcctgaat ttggctggtt gaaaggtatc tggtacagtt tgttccatgc aatctctagt
    65041 ttttgtaatg cagggtttga tttgttagga gacagtttag ccaatcacca gcaaaatgtt
    65101 tatttgatca tggtggtatc tgctttgatc atttctggtg ggttaggctt catcgtatgg
    65161 cgtgatttac tggaatatca tcagaaaaag aagatgacga ttcattcaaa aatcgcatta
    65221 accgttacag gtaccctttt attaggcgga ttcgttgtgt tctttttcac agagcgcaat
    65281 ggggcaacgc tagtggaagg gaatatgttt caacgattgg caaacacctt ttttatgagt
    65341 gtgacaccta gaacggctgg atattattca gttgattacc tccatatgag ccatgcaggt
    65401 ctgattttaa cgatgttttt gatgtacatc ggcggaacat ctgggtcgac ggccggcgga
    65461 ttgaaaacaa ctacgttagg tgtgttgctg atccagatgc gttcgatgtt gaaaggacgt
    65521 acgagagcgg aagcatttgg ccgtaccatc cgacaaggcg cagtgttgcg ggcacttact
    65581 ttattttttg ttacgttgac gttgtgtgta gtcgccatca tgattttatc ggtgactgaa
    65641 acgataccta aaacttctgg catcgaatat attgcttttg aggtcttctc tgcgtttggt
    65701 acagtaggtc ttacgatggg attgacgcca gacttgactg caatagggaa attgatcatt
    65761 atagcgctta tgtacattgg acgagttggt gtattgactg tggtcttctc tctgatgaca
    65821 aaagcacaaa gaacacaatc aaaatacaaa tatccagaag aaagtgtcat gattggataa
    65881 tttcatcttc tgacagaaac ctagtgattt agtaaaataa gaaaataaaa taatttttct
    65941 tgcttcttgg atactttaag tgcaaaatgt tcgtttattt tttgaggcac tacccgttgt
    66001 tgaacctcgg gtagtgcctc ttttttattg aaaggaatca ataagatttg attcctttat
    66061 ttatcaatag gttataattc atattttttt attttttctt ttacgatact tagtccagaa
    66121 gctaaaattt caaggttatt agaggatagg gaaattcgaa cgaatgcatc tggttgcgca
    66181 cgtgtagtaa agcgtctgga atgataaagt cggataccgt tagcaagaaa gtcattctca
    66241 attatcttag ggtcaatctc aggatcaact ggaagaattc gatagtaagg gacacgattc
    66301 gttggttttt ctactttgaa aagagaatca aaaagctgat tggctctttt tgtaagactt
    66361 aatttagttt ctaagatcct atttgcttca tttgaatgga ggatttgagt gatgacttcc
    66421 acatctaatc cagaagtttt cacatttata ttaaaaattg cttgttctat cttgctttgg
    66481 acatgtactg gaactacaag ataagcaact cgcaatccag aacaaataaa ttttgtcatt
    66541 ccagctaagt agattgtttg gttaggtaat aaattttgga aaggaggaat gatcgtatgt
    66601 tgatagtaag ttgttaaaaa tgaatggata tcatcttcaa taacccatag accttctttt
    66661 tcaatcactt cggctaattg ctctcgtctt ttgtgagaga tttgaaaccc tataggatta
    66721 ttacaagaag gcataagaaa gacaccatga atcttctttt ttctacattc ctgaatcaat
    66781 atatcaggtc gcattccttg ctcgtcaaaa tcaattggaa cgatctctaa atgatataac
    66841 tgagccaatt caatgaaatt agcgtatgta tatcggtctg ttgctattct atcacctgga
    66901 gagaaaagtg cagaaagtgt gatagccaat ccattttgaa caccggaaac aatagataga
    66961 gattctaagg acggaatgac tccttgagtt tttaaccaat ttgctgcagt agtcaattga
    67021 aaatttgtgc ctttgggatc ttggtaatta agcaaaccaa caagatttgg gttattacta
    67081 gttgattgaa taaacgggac aacgaattta ttacattctt caaaagaact aaccagacca
    67141 aaatcaatga tatgttcggt tacttcattg gtagagatgg tgatggactc aaagacattg
    67201 ggagaattaa atgtgccact tccaatattc gtatacagca aacctttctc aattccatat
    67261 ttataagcct tgccaacggt agtaaaattt aaatctaaat aatcagctag ctctctttgg
    67321 gagggtaact gagtgttttt ctgtaattta ttctttaaaa tatcttgttc taattgttga
    67381 atcaacgatc gataataagg acgcttcaat ttttgtttgt ctggtcgcca agttaacggg
    67441 taattgtcat aagaattaat aggcattttc tccactcctt aaaattgtat accatacaat
    67501 tttaccattg attgtattga ttttagaagt taatcttttg tgaggaggga attgtctatg
    67561 aaaattatta attttgagga agtagtagct tcttttagtt ttgaagaagc gattcaagta
    67621 atgaaaaaat gtttttttga ttatgaaagt ggtaaaatca gccaaaatcc tcgattggtg
    67681 gaaattttac ctgacggaaa aggcaaaaat atgtttgcga tgatgcctgc ttatttgggg
    67741 gagaacagat attttggcac aaaagtcatt accgcttttc cggataatca tcagtatcat
    67801 ttaccttctc atttaggaga gattttcttg tttgatgctc aaaacggact acccaaagca
    67861 ttgataaacg caaatgcagt cacctggatt cgaacagctg ctgtatctgc gctggcaaca
    67921 gattacttag caaatccgca tgcagaaaca cttgctttaa ttggagctgg tcaacaagca
    67981 ttgtcgcatt tgactgcgat ttctaccatt cgtcccatca ctacagtgtt tgtttacgat
    68041 attttagaag aaagacgaaa tacttttatc gctaaagcta aaaaacagta tcctaaaata
    68101 acgtttataa atgcaatatc agtagaagat gcagtacgtc cagcggaaat catttgtacg
    68161 ttgacctcta gtaaagaggc atttcttcaa aaagagtggg taagggaaga cgcacatatc
    68221 aatgctgtgg gcacatttac tcctgataca agagagataa gtacatcgtt gatggaaact
    68281 tctcaaatct atgtagatga ttactcagct gccttaacag aaagcgggga cctgcttata
    68341 gccatggcag aaggagattt agaggaatct tgtattgttg gtacattagg cgaattagtt
    68401 tctgagaaaa aaacatttta taaaggagaa tcgaggccga cgattttcga cgcagttggt
    68461 ctagctattg aagatctttg ttgtgcagaa tatatttata acaaattaga gaaaggggct
    68521 ttaaaatgaa cttagattat tacgtagttg atgcatttgc agatgaggtg ttcaaaggaa
    68581 atccagcagc ggtttatgtg ctagaagaat ggctgccaga aggtacgatg caaaaaattg
    68641 ccattgaaaa caatttatct gaaacagcat ttacagtgaa gaaaaaccaa gaatttgagc
    68701 ttagatggtt tacgccagat cgagaaatcg atctttgtgg tcacgcgaca ttagcaacag
    68761 catttgtttt atttaactat tacaaaatac ctgatgaaac gattaaattt tctactcaaa
    68821 gcggtaatct atttgtgaca agaattaaag attattatta catggatttt cctagtatta
    68881 tgccgaaaaa agtgccgata ttgtctgaat atgaagaagc gattggggct aagattaaag
    68941 aagcctactt agcacgtgat ttatttttcg tattagaaga tgaaaaaacg gtggctaaat
    69001 tgcagccaga ttttacagct atcaaaaatt ttgaattagg aataggagtc atcgtgacag
    69061 cagaaagtgt gcaaaaagac tttgtttcta gaaccttctt tccaaaatta acaatcaatg
    69121 aagatccagt atgtggatca gcacattcta atcttattcc ttattgggca gaaagaatga
    69181 ataaaacaaa attgtcagct tatcaaatgt catcaagggg cggggatcta gaatgtgaat
    69241 taaaagaaga tcgtgtgata ataggaggaa aagcagtttt gttttctaaa ggtacctcgt
    69301 ttatttgatt tctttcaaat gaattaaaaa taaattggtg gagagtggca accttatagc
    69361 tacaatggtt gttaaaaaaa gacgcaaact ttttcaattt aatgaaaagt ttgcgtctca
    69421 ttttaattat tggtgtttgt tttttattca atcaagagta aaatgcccca tactaactat
    69481 cctcggatga cttcgatttt ataaccatca gggtcaatga caaagtagta agaaggcgca
    69541 gtgccaggta atccttttaa gtcagtaatc tcaagaccgg cagctttatg ttgctcgtgt
    69601 aatttttcta gatcatcagc tgctatagcg atatgaccat agccgtctcc tagatcatac
    69661 gcaccatgat catagttgta agtcaactct aattcatagt catccccagg aagtgtcaaa
    69721 taaacaagtg taaatttgtt ttctggaaaa tcacggcggc ggctttcttc aaaaccaaat
    69781 gccttttgat aaaattcgat tgatgcttca agatctttca ctcgaacaca tgtgtgagcc
    69841 attttcatag attcatctct ccttttcctt ttactcaatc ataacaaaaa gaagtaagga
    69901 aatagttgat ttgtgcttgt tcatgagaaa tttctcatgt aaaatggata gaaaataaaa
    69961 ctgttaaggt ggaaactatt catggataaa ccaatttttg gtgaaaaaaa gcaatttgct
    70021 gattatcaaa cccgttatgc agcatatatt gtcatagaaa gagatcagca gattgcagta
    70081 atcgaggctc caaatggtgc tttttttctt cctggcgggg aaattgaagg agaagagacg
    70141 aaagaagaag cgattgtccg tgagctgata gaagaaatgg gaattgtggc agaaatttct
    70201 tcttatctgg gagaagctga tgagtatttt tactcgaatt atcgtgcaac ttattattat
    70261 aatccaggat atttctttgt tgctgattct tggaagcgta taggagaacc aactgaaaag
    70321 accaataaaa tttggtgggt gagccctcaa gaagcgcttg tgaaattgaa acgtggtagc
    70381 caccgatggg cagtcgagag atggatcgaa aaaaataaaa actgaaagaa agcgagtaga
    70441 tcagctttct ctcagttttt ttatttatta cagtacatat tttttgattg cttcagctac
    70501 accgtgctcg tcgtttgatg aagtgatgac atttgctgct tctttgacaa gagaaacggc
    70561 atttcccatt gctactccta gaccagcata ttcaatcatg gataagtcgt tctcgttatc
    70621 tccgatagcc atgatttcat cttgatcaat accaaggtgc tgtgcgagat tcgcaactgc
    70681 cgcaccttta cttgcttctt tatttagtac ttcgaagtaa tagggcgcac ttttgaccgt
    70741 tgtgtattta tcacggaaag tttgcggtaa acgcgcaatt gctgcatcta aaatttctgg
    70801 ttcgtcgatc atcatcattt tgacgatgtt catgtctggt gtcatttctt caggtgttcg
    70861 atattttagc ggcatcttga ctaaacttgc ttcgtggatc gtataagcgc tgatatctcg
    70921 attaggcgtg tagatcgctt tgtcatcgat cgaatggaga tgactgccga cacggcgtgc
    70981 catcacttcg atctctagga aatcttcatg tgtcagtccg tgacgggcga tgattttacc
    71041 tgatttcgtt tgttgtacga gtgccccgtt ataagtaata acgtaatcat tatcttgaaa
    71101 aaggttcaac tcgtttaact gttcctggac acctggtaag ggacggccag tgcataaaac
    71161 gatcgaaaca ccttttgcaa cagcagcggc aatcgcttct tttacttctt ttgttacttc
    71221 tcttttctca tttaataaag taccatcgat gtcgatagcg actagtttga tagacataat
    71281 ttcctccaat atattttaat ttatttcaat caatgcgcca ttacggatat ggctggcaaa
    71341 ttcttgataa gtttcgtcaa ataaatcata atgatcacgc aaggacggat caaccatttc
    71401 ttttggaaag aaaaatcgct catctccttg ttcttgtccc gctaatgcag caacaagctg
    71461 actaacctgt gataattcta tcaaagttcc gtcatttcgc aaaatctcga tttgtgtgcg
    71521 atgtctgcct tgttctgggc gataaaaatc ataaggtaga tcgtagctag aattcacagc
    71581 agtgtaataa acaggattat atccgacctt ttctactaac aaagtgagtt cttggactaa
    71641 ttttgaatcc cggttgccag aaaaagtagc agatttcagt ggtttacgat tcaaaaaacg
    71701 tttagctaaa tcatttaaaa taggatcagc agattcagac cattgagtaa agtaagtgcc
    71761 caagacccca tcatcaagtt tgaggtattc ttctaaagta aagcttccgt caagaaatgg
    71821 cagaagtaat tgagaatgga gttcaaaact gctggcatct tcttgataca actcgtgtgc
    71881 gcgatgaagc agatgctcca aaattacttc cataccacgg gaaacaggat ggaagtacac
    71941 ttgcacatac atttgatatc ggctaacgat ataatcttcc actgcatgca tgccactcat
    72001 ggaaaaagcg atcccgcctt cataaggacg aatcacacgt aatattcggg taagatcaaa
    72061 tgttccatat tctgttccag tgaaataagc atcccgaagc aaatagtcca ttcgatctgc
    72121 atcgatctga ctagaaatca tttggacaac ttgtgggttg gggtaagttt tttggatcac
    72181 actagctacc ttttcaggaa aaccttcttc tactcggtta agaatctgat acacttctgt
    72241 ttctggagaa gtgatgatct ctacagtgat ggcttcatga ttcgtatgga agatatgttc
    72301 aaatgtatga gagtaaggac catgaccgac atcatgcaac agtgccgcac ataaagtcac
    72361 cagccgttcc tgatcatccc agcctccgtt gcctattttt tcttttgaaa aatttcgaga
    72421 aaagatatcg cagattcgtc ttgaaatctc ataaacgcct aatgaatgag taaaacggct
    72481 atgttctgct ccgtgaaaag tgaaagaaga agtgcctaat tgtttaatgc gtcgtagacg
    72541 ttggacttct ttcgaattga tcaaatctaa gattacttga tgctggacat ggatatagtt
    72601 atggactgga tcacgaaaaa ctttttccat aggcagcatt tgatatttat aaggggttgt
    72661 cataatgtag ctcctttatg ttattacgtt gttccattct ctggatttgg ttgttccatt
    72721 gctccagctg gatttctttt tttagccagt cggtagaatc ctgtaaatca cttgcagaat
    72781 acagcttttc aaaagctttt gcaaatgctt ctttgacttc ttttacaaaa aggggttggt
    72841 caagcagttc ttcaagtgtt gccattgtag caggatccac tgcaggatag ccatttgtac
    72901 caaagtcttc ttttaaagca gattgataaa atcgcttcat gacttgcccg cgtttttcct
    72961 gattaccgtt gacacttaag tacatcataa cagaaatccc atttttgatc cgacgctgag
    73021 cgataccggc aaattttttg ccatggatgc ttaaatcata agttccagga caataagaat
    73081 gggtgatttc gaatgcttgt atcggtgcct ttggaaaagc gtatttagtc aataggagca
    73141 tttgctcgta agcgtcatct atggttattt tttttgtgtc actttggggg aaaatcaaag
    73201 aaatattcaa aattccttca tctgatacga tccctaatcc acctgaattt ctgatcactg
    73261 gagcgtaacc tgcatctttc agtgtttgga tcccgtcact tagaaaagga gttcgaacat
    73321 ccttcatacc taagatgact gtgcagtcat attgccagaa atgtaaaaag atttgctggt
    73381 gttctttaga atattccgtt aatatatctg ttaatgcaaa aggggaaaag tcgttttttt
    73441 gataaatgtg ctgatctagt cgaatcgcag ggttcattca accggctcct ttgtataaat
    73501 tcatattttc tacaatatta tactctaaaa tcaaagagtt gttttggaaa aaaggttttc
    73561 tgtaaaaatg gacaagttgg ttgacaaaac aaaagaaact aggaacaatg tagctatgag
    73621 aagtaaagga gaatgaataa tggaattatc acaaggaaca ccgatttcaa tcaaattgcg
    73681 tacaaaagta aaacagcaag gggaagtgca agatttttat tttgatttga aagggcaaat
    73741 ggtgacaatt ggcgatacgc tgtacatacg ttataaagag attcaggagg agacgggcga
    73801 tgagattcct gtaacgatca aattagttcc agatggtcat gtacagctga tccgtgcagg
    73861 agaaatgcgg atgcgtttga aatttggcta taaagaacgg ttagaaacat cctatcgaac
    73921 accatacggt atgatccaga ttgctacttt tacgaaagaa ctgcatgtta gtttgaaaga
    73981 ccgtcccaca ggagggaagg ttcgaatcga ttatgatctg ttcatgggac cggaaaaaat
    74041 cggcgaatat tatttgacct tggattttac agcgtaaata taaaagaaaa gtttgatttt
    74101 ttgtgtctga ttttgtatga taaggagtag actgtgaaag gacgtgtcct gattggaaat
    74161 tagtgtattt gaaggtgcaa acaaaagcga attatcaatg atcgaagtag cacacgcgat
    74221 cttagaacaa cgtggcgatg tcatggattt ttctgattta gctaaccaga tccaaaacta
    74281 tcttgggaaa tcagacagtg aaattcgtga ttcactcgca caattttata ctgacttgaa
    74341 tattgacggc agctttatct cattagggga taatcgttgg ggcttgcgtt catggtatgc
    74401 tatcgattcg atcgatgaag aagtaaacca tggaatggac gaagaggatg aagatactcc
    74461 acgtcgccgt aaacgcaaga aagtcaacgc ctttatcaat gatgatgaag acgcgatcga
    74521 ttacaatgac gatgacccag aagatacaga tctgacagaa gaagatgacg atgatctgtt
    74581 tgatgacgat gatgatgaag atgaagaaat cgctgcttat aattctgact tgcaagaaat
    74641 cggagcagat gacccgacag atgatgaaga agacttgcca ggaaatatcg aagaagattt
    74701 aagtatcata gacgaagacg atgatgatga agattatgaa gaggaagaat tttctgactc
    74761 tgaaaaagaa taaacattat tttcttacga gaggttgtga cagagacgat cagcctatca
    74821 gaataaggag gtgtgacacg atttttgtca caccttcttt tttgtatata aaaatcccaa
    74881 gtaaatacat aaaaaataaa aaaattgaaa cttttagcgc accttgtacg tcatattaag
    74941 tgaggcagaa aggagtggag aatgattttt actggatata agaaaaaacg gcagatcgaa
    75001 aaataccgtc acctgctgaa tggactcatc gaaactgatt atcaaaaaat gttccgtatc
    75061 acgatgaatt acgtacatga taaagaagag gccttagatg tgatgcaaga tagttttcat
    75121 aaagccctga actattttga aaaagaaaaa gagatcgagc attttagtgc atggttttat
    75181 cggatattga tccgcacggc attagatagt tggcgcagac aaaaaaggac gcctgtggac
    75241 ttatttgaaa ttcaagatat cccccaacag ttacctttag aaacttccgt tgcagagctc
    75301 catatgattt tagataaaat agacagtccg gaaaaagaga ttctgatcct ctactttttt
    75361 gaaggatttc gtttgaaaga aattgcgact atcttggata tgaatgaaaa cacagttaaa
    75421 acgaaaatgt accgctcatt gaatgcttta cgtcaaatcc tagaatgaag gagagaagag
    75481 tgccatgtat tcccaaaaag aaaatttaaa aaaactaaag aaaacttata aagaacaagc
    75541 aattcccaaa gggcttaaag aggagatccg ccagcgtttt attatcgaag agcaatcatt
    75601 tataaaagca aagcggagaa agagacgtct ccaagggttc ggagtatctt tggctatcgt
    75661 aatgattaca acaagtagcc ttctttttaa tagtcaagtt cgaagctttg cagaagattt
    75721 acccatctta ggatcagtca ttgaattgat cttaggcgaa cgctttactg atcgatctga
    75781 aaaaatcgat attcaggtac cgaagatcaa tacacaaaat gagaaagaaa ataaaacaat
    75841 tcatggatta aatcaaaaat actttcgcga aggacaagca gattttgaaa aagctgaaaa
    75901 agaatatgga aattttgaaa cggatcatta ccaagttttg ggagattatc agaaaatcgt
    75961 agacgataac cggtttctcg tgattgaaag gcagatcacc caaacagcag cagatagcca
    76021 tgttgaaaaa agatacgaca caatcgacaa gaaaaacagt gtccagctct cacttccact
    76081 tttattcaaa gatgacacct atctatctgt tttgacaaaa gaagtcaaac ggcagatggc
    76141 cgaacaagtc aaggaagatc catctaagta ctattggact gagcaagata ttcaagaagg
    76201 gacgatagaa aaaccaacac ttgttacgcc tacacgaagt ttttatttga acaaagagca
    76261 tcaattagtt ttgacttttt ctcaatacga aattgctcct ggttatatgg gtacaccaga
    76321 atttgtcatt cctaagtccg tgacaaaaac gattttggca tcggaagatt atttggatca
    76381 ttagacgaga agtagttgcg actagcagtt tcaattttca agtaagtaca agtaaataat
    76441 aagaagagaa aaagagaaag aagagatata gtgaaaagaa attatttagt tatcagtatt
    76501 attgctttat ccgtgctttt gtttttcgaa attacttcgg tggcctcctt gatcaccgaa
    76561 gtaaaaggac aaacacactc ttcggagcat tcgtcacaat ctttcaaaca atcagaacaa
    76621 tcaaccatta aaagccaatc aactgaaaca gctaacgtca aagaagccat tcaagaatca
    76681 gcggaaaagg tacaaagtca gtcatcacaa tcaacacaag acagtacaaa aacgagccga
    76741 gatccggctg tcaaaagcgt cgcaatcagc tttgatgatg gaccaggagc gaccacaaca
    76801 ccgcagcttc tgcgaatctt aaaagaaaaa aacgtgcatg ccactttttt cgtcttagga
    76861 gaaaatacgg cgcagcatcc tgaaatcgtg aagcagacag ctgaagcagg gcacgagatt
    76921 ggcaatcaca catacgatca tcagaatctg gccactcttt ctgcccagtc gatgacggag
    76981 gaagtgacga aagcagacac tgagatcaaa aaagtcactg gcaaaacccc cacttttgtt
    77041 cgtccgccct atggttctgt cacgaatgta ggagcaacga tcatccagcg accgatcatc
    77101 gaatggtctg ttgattcaga agattggaag acaagaaatc ctgatttgat tttgcagaag
    77161 atccaagcaa cagtatacga tggggcaatt attttattcc atgatatcta tcctgaaacg
    77221 attcgtgccg ttcctcaagt gatcgattat ctgaaagagc aagggtatcg catcacgaca
    77281 gttggggatt tattagggca tccaacggct gtcgagaact attatggcag aaacgatcat
    77341 cggccagttc agtaagtgag acaagtatct ttaataaata acacatttta ataagtagtt
    77401 gaacacataa ctaccttact tgcaaaggct taacaatatc tatacaggta caacaaaaaa
    77461 ctgtccacca attttctttg gcggacagtt ttcacttaaa aataaagaag caactattcg
    77521 taaattatgc taaaatagca gtagaacgcg tcctgaggga atcgaacccc cgtctcaaga
    77581 accggaatct tacgtgatat ccactacact aaggacgcaa gcactgggat aattgtaact
    77641 aaacagataa aaaattacaa ggggattgga caaaaaaatg aaatttgagc aaaatttttc
    77701 acaaaaacag cagcagacac agaaactagc catgacgcaa cagttgcaac agtccatcca
    77761 agttttacaa tataacagtg aggagctgtt ggcattcgtt gagaatcaag caatggaaaa
    77821 tccattggtg gaagttgttg agccggaatg gcagccagat tacataaaag cttcttcatc
    77881 ttcttatgaa ggagaagaga caaattacct aaatcagatt cctgatacaa aagggtcttt
    77941 atttgattca ttgatcgaac aagtccattt gaattaccga gatacttttt tgaggaaaat
    78001 cgttctttat cttgtagaat acatcgattt gaacggattt ttgacgatta gtttagaaga
    78061 agccggaaaa gagattggag ctacgcctat ccaaatgctc gatgcgttga ctctgattca
    78121 gcaattagaa ccagctggag taggtgcacg tagtttgcaa gaatgtctca tgcttcaaac
    78181 agaacgagat gattatgcgc cggaattagc ttatatcgtg ttggaagaat gttttgagga
    78241 actggtggag cgaaaatgga aagaaatcgc acaacgtttt tctgttgatc tacatgctgt
    78301 tcagcagata ttcgactatt tacaaacgtt gagtccaagc cctggccgga ttttcgaccg
    78361 tagcagcgaa ttgtacatta ttcctgatgt ccgggtatta gtggacgacg ataaaaatgt
    78421 acaagttatc tcaaaccgaa aaaatcagcc aaatatacgc tttcaagaga gttacttcaa
    78481 acaaatgtca caacaagcag ataaagaaac tgaaaattat ttaaaagaac ggaaacagga
    78541 attcgaatgg ttgaagaaaa cgatcctaca acgtggggat acaattttgc gggttgctca
    78601 agtcattgtt tcccggcaaa aagctttctt tatcgacaag gaacgtccaa tcaagcctct
    78661 tacgttaaag gaagttgcaa ctgagataga tgtgcatgaa tccacggtca gtcgtgcagt
    78721 caacgggaaa tatttggaaa cagacttcgg tgtatttgaa ttaaaaaaat ttttcactac
    78781 acgaattcct acgaatagta cagaacagac agaagacctt tcagcagata cagcgaagaa
    78841 aaagctgcag gaattagtcg atcaagaaga caaaaacaag ccgttgtctg accagaaatt
    78901 ggttgaattg ctgaaaaaag atgagatagc gatctcaaga cgaacagtag caaaatatcg
    78961 tgatttatta ggaatcccga gttcatcaaa aagaaaacgt tatgataact aatcagtcaa
    79021 aagactagaa aaagtatttc actttttcta gtctttttag ttgaaaaacg agagcaaaac
    79081 tgttagactt acagtcgtga ggttgagatt tccctttatt gatgtgaaat tcgaatagtt
    79141 aatttctttt tttatcgtat atgggtcgtt ttaagtctac attggacgta aagtgtccaa
    79201 ctaaggagat cgctatgttg gatgaaataa aaatgattga atctgttgca ccagatatta
    79261 tcgaagtgtt gcaagaacgc tataaaattt tacgaaatat ctattggatg cagccgattg
    79321 gacgtcgaag tttatcggaa agtatgggga tcacagaacg agtattgcga acagaaacag
    79381 atcttttgaa aaatctgaat ttgatcgaca cctcaaaaag cggtatgacg ctgacaacta
    79441 agggcgaaga agtttatcaa agccttgaaa acttcatgga tcagctttta ggtacgcatc
    79501 aaaccgaaca acagttggca gaatattttg gtaaccaacg ctgtatcgtt gtttcaggaa
    79561 acagcgaaga gcagaccaaa gtggctgatg cattcggcga agcgctgagc gaagccctag
    79621 accgactact tccagaaggc gagaatatca tcgctgtgat gggaggaact actatggctg
    79681 tggtagccga acaattgtct aatctggaaa ataagaaacg gcataacctg tttgtccctg
    79741 caagaggcgg gattggtgaa gcgataactg tacaggcaaa ttctgtcagt gcaagaatgg
    79801 ctgctaaagc aaacggtaac caccgtgcac tttatgttcc ggaacaatta agtctggcaa
    79861 cttacaattc tttactcaat gagccatcta tccaagaagt cttaaacttg attagtgaag
    79921 cgaattgtgt tattcatagt attgggcgtg cattacacat ggcagcacgt cgtaagatga
    79981 ctgaaaaaga attagtcatg ctgaaacaag caaatgctgt tgcggaatct tttggctact
    80041 ttttcaatga aaaaggtgaa gtcgtttata aggtccctag aatcggcata gaattgcaag
    80101 atttggaaaa agtcccgatc atcatggcaa tcgcgggcgg aaaatcaaaa gccaaagcaa
    80161 ttcgagctta tatgaaaaat gcaccaaaac aaacgtggct catcactgat gaagctgctg
    80221 caaatgagat tttaaaaggg gtaacccttt aaaataaata tttttttgat tttcataagg
    80281 aggaaatctt aaatgacagt taaagtaggt attaatggtt ttggacgtat tggacgtcta
    80341 gcattccgtc gtatccaaga tgtagatgga atcgaagtag tagcaatcaa cgacttgaca
    80401 gatgctaaaa tgttggcaca cttgttaaaa tacgacacaa ctcaaggacg tttcaacgga
    80461 acagttgaag ttcatgaagg ttcattcaac gtaaacggaa aagaagttaa agtattagct
    80521 aaccgcaacc cagaagaatt accatggggc gaactaggcg tagatatcgt tctagaatgt
    80581 actggtttct tcacttctaa atcagcagct gaaaaacatt taacagctgg tgctaaacgc
    80641 gttgttatct ctgctcctgg cggaaacgat gttccaacaa tcgtttataa cacaaaccac
    80701 gaaacattaa ctggtaaaga aacagttatc tcaggtgctt catgtactac taactgttta
    80761 gctcctatgg ctaaaacatt gaacgacaaa tttggtgttg ttgaaggatt aatgacaact
    80821 atccacgctt acacaggtga ccaaatgact ctagacggac ctcatcctaa aggtgacttc
    80881 cgccgtgcac gcgctgctgc tgcaaacatc gttcctaact caacaggtgc tgctaaagct
    80941 atcggtttag taattcctga attgaacggt aaattagacg gagctgctca acgtgttcct
    81001 gtaccaacag gttcattaac agaattagta acagttcttg aaaaagaagt aactgttgac
    81061 gaaatcaatg cagcaatgaa agaagcttca aacgaatctt acggatacaa cacagacgaa
    81121 atcgtttctt cagatatcgt tggtatgact tacggttcat tatttgatgc tacacaaact
    81181 aaagtaatga cagttggcga caaacaatta gttaaaactg ttgcttggta cgacaacgaa
    81241 atgtcataca ctgcacaatt agtacgtact ttagaatact tcgctaactt ataagataga
    81301 caaacgtaca agtgtaaatt atagacatgt acatttaaaa gcggggaagc aatcgcgctt
    81361 cttccgcttt ttttgataat aagtttattc aggaggaaat aaaatggcta agaaaacagt
    81421 gaaagatatc gatttaaaag acaaaaaagt tcttgtccgt gttgacttca acgttccttt
    81481 gaaagacggc gtgatcactg acgacactcg tatcaaagca gcattgccaa caatcaacta
    81541 cgttctagaa caaggcggaa aagcaatcct tttctctcac cttggacgtg taaaaacaga
    81601 agaagataaa gaaggaaaat ctttagctcc agttgcaaaa cgtctaggcg aattattagg
    81661 taaagaagta acattcgtac ctgaaactcg cggagaacaa ttagaagaag caatccgcaa
    81721 catgaatgac ggcgacgttg tcgtattcga aaatacacga tttgaagata ttgacggtaa
    81781 aaaagaaagc aaaaatgata cagaactagg taaatactgg gcttcattag gcgatgtatt
    81841 tgttaacgat gctttcggta cagctcaccg tgcacatgct tctaacgtag gtatcgcttc
    81901 aactggtatc ccaacagttg ctggattctt gatggaaaaa gaaatcaagt tcatcggtga
    81961 agcagtagaa gaaccaaaac gaccaatgat tgcaatcctt ggtggcgcaa aagtttctga
    82021 taaaatcggc gtaatcgaaa acttgattcc taaagcagat aaaatcttga tcggtggcgg
    82081 aatgacttac acattctacg aagcaaaagg aatcaagatc ggtaactctc tagtagaagc
    82141 tgataaagtg gaactagcaa aagaattaat cgaaaaagca ggcgacaaac ttgtcttgcc
    82201 aatcgacaat gtctgtgcac cagaattttc aaatgatgtt gaaacacaag taatcgaagg
    82261 agacattcct gatggtttga tggctttaga catcggccca gcatcagtaa aattatttgc
    82321 tgatacatta caaggtgcaa aaacagttgt ttggaacgga cctatgggtg tatttgaaat
    82381 gagtaacttt gctaaaggta caatcggtgt ttgtgaagca atcgctaact tagaaggcgc
    82441 aacaacaatc atcggtggcg gagactcagc cgcagcagca gaacaattag gatttgctga
    82501 caaattcaca cacatctcaa caggtggcgg cgcaagcttg gaattgcttg aaggtaaaga
    82561 attaccagga ctagcagcaa tcaacgataa ataaacgacc aagtttgctc aattctgacg
    82621 agtaatcgat caaaaggtga agggagtttt catgactccc tagccatttt tcaataaatt
    82681 aaggagtgtg ttcctcaatg cgtaaaccta ttatcgcagg taactggaaa atgaacaaaa
    82741 ctgcatcaga agcaaaagaa tttgctgaag cagtaaaaaa caaaatccct tcaaatgaca
    82801 aagttgattc agtcatcgga tctcctgcat tattcttaca agaattaatg acatcagctg
    82861 aaggtacaga actaaaaatc tcagcacaaa actgctactg ggaaaactca ggtgcgttca
    82921 ctggtgaaac ttcaccagca gctcttgctg atctaggtgt tcattatgtg atcatcggac
    82981 attcagaacg tcgtgaatat ttccatgaaa cagacgaaga catcaataaa aaagcgaaag
    83041 ctatctttgc aaatggtatg acaccaatcc tatgctgtgg cgaatcttta gaaacctatg
    83101 aagcaggcaa aactgctgaa tggatcgaag gacaaattac agctggttta gctgatcttt
    83161 cagctgaaca agtgtcaaac atggttattg cttatgaacc aatctgggca atcggtactg
    83221 gtaaatcagc tgacgcacaa atcgctgacg aaatttgtgg agttgtacgt caaacagttg
    83281 aaaaacttta tggaaaagaa gtatcagaag cagttcgtat ccaatacggc ggttctgtaa
    83341 aaccagaaaa tattgctgaa tacatggcaa aagaaaatgt tgacggtgct ttagtcggtg
    83401 gcgcaagctt gcaagcagat tcattcttgt cattgttaga cgctgtaaaa taattgttga
    83461 aaatttgcga aaaatgcaag aaatgtttgc actaatgagc aaaattaact aaaatcgtat
    83521 atgagggaaa tcccttagga aaaaacaaac tcaaaggaga gacaaaacat gtcaattatt
    83581 actgatgttt acgctcgcga gatcttagac tcacgtggta acccaacaat cgaagtagaa
    83641 gtatacacag aaagcggagc ttttggccgc ggaatggttc cttcaggtgc ttcaactggt
    83701 gaatacgaag cagttgaatt acgtgacgga gacaaagctc gttatggcgg taaaggtgta
    83761 actaaagctg tcgacaacgt aaacaacatc attgctgaag caatcattgg ctacgatgtt
    83821 cgtgaccaaa tggctatcga caaagctatg atcgctttag acggaactcc taacaaaggt
    83881 aaattaggtg cgaacgctat tttaggtgtt tctatcgctg ttgcacgtgc agctgctgac
    83941 tacctagaag tacctttata ccactaccta ggcggattca acacaaaagt attgccaaca
    84001 ccaatgatga acatcatcaa cggcggatca catgctgata actcaatcga cttccaagaa
    84061 ttcatgatca tgcctgttgg cgctccaaca ttcaaagaag ctctacgtat gggtgctgaa
    84121 gtattccacg cattagcttc aatcttgaaa gctcgcggat tagcaacttc tgttggtgac
    84181 gaaggtggat ttgctccaaa ccttggttca aacgaagaag gttttgaagt aatcatcgaa
    84241 gcaatcgaaa aagctggcta tgtacctggt aaagacgttg ttcttgctat ggatgctgct
    84301 tcttcagaat tctacgacaa agaaaaaggt gtttacgtac tagctgattc aggcgaaggc
    84361 gaaaaaacaa ctgacgaaat gatcaaattc tacgaagaat tagtttctaa atatccaatc
    84421 atctcaatcg aagacggatt agacgaaaac gactgggacg gattcaagaa attaactgac
    84481 gtattaggcg acaaagttca attagttggt gacgacttgt tcgtaacaaa cactcaaaaa
    84541 ttatctgaag gtatcgaaaa aggaattgct aactcaatcc ttatcaaagt gaaccaaatc
    84601 ggtacattaa cagaaacttt cgaagctatc gaaatggcta aagaagctgg ctacacagca
    84661 gttgtatctc accgttctgg tgaaacagaa gattcaacaa tctctgatat cgctgttgca
    84721 acaaacgctg gccaaatcaa aactggttct ctatcacgta ctgaccgtat tgctaaatac
    84781 aaccaattac tacgtatcga agaccaactt ggtgaagttg cagaatacaa aggtttgaaa
    84841 tctttctaca acttaaaaaa caaataattc ttttctaatg aagatacatg atcgactatt
    84901 gaaagatagt cgagtatgat cacttagtta agtaatcgaa ctctctactt gctgaaatat
    84961 cagcaggtag agag
//
LOCUS       DAFEPQ010000006        81535 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.006, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000006
VERSION     DAFEPQ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 81535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.006"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            12..590
                     /locus_tag="LP543_000431"
     CDS             12..590
                     /locus_tag="LP543_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320624.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(830..913)
                     /locus_tag="LP543_000432"
     tRNA            complement(830..913)
                     /locus_tag="LP543_000432"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(877..879),aa:Leu,seq:gag)
     gene            968..1147
                     /locus_tag="LP543_000433"
     CDS             968..1147
                     /locus_tag="LP543_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320625.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            1296..2513
                     /locus_tag="LP543_000434"
     CDS             1296..2513
                     /locus_tag="LP543_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL2320626.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2697..3533
                     /locus_tag="LP543_000435"
     CDS             2697..3533
                     /locus_tag="LP543_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2320627.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3609..4757
                     /gene="nagA"
                     /locus_tag="LP543_000436"
     CDS             3609..4757
                     /gene="nagA"
                     /locus_tag="LP543_000436"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBL2320628.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4978..6036
                     /locus_tag="LP543_000437"
     CDS             4978..6036
                     /locus_tag="LP543_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBL2320629.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6040..7074
                     /locus_tag="LP543_000438"
     CDS             6040..7074
                     /locus_tag="LP543_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBL2320630.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(7176..7892)
                     /locus_tag="LP543_000439"
     CDS             complement(7176..7892)
                     /locus_tag="LP543_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL2320631.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(7861..8646)
                     /locus_tag="LP543_000440"
     CDS             complement(7861..8646)
                     /locus_tag="LP543_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HBL2320632.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8637..9386)
                     /locus_tag="LP543_000441"
     CDS             complement(8637..9386)
                     /locus_tag="LP543_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBL2320633.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9684..10208
                     /locus_tag="LP543_000442"
     CDS             9684..10208
                     /locus_tag="LP543_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBL2320634.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10224..11723
                     /locus_tag="LP543_000443"
     CDS             10224..11723
                     /locus_tag="LP543_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBL2320635.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11811..12413)
                     /locus_tag="LP543_000444"
     CDS             complement(11811..12413)
                     /locus_tag="LP543_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL2320636.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12517..13473
                     /locus_tag="LP543_000445"
     CDS             12517..13473
                     /locus_tag="LP543_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBL2320637.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13778..14734)
                     /locus_tag="LP543_000446"
     CDS             complement(13778..14734)
                     /locus_tag="LP543_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL2320638.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14908..15468
                     /locus_tag="LP543_000447"
     CDS             14908..15468
                     /locus_tag="LP543_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320639.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15461..16285
                     /locus_tag="LP543_000448"
     CDS             15461..16285
                     /locus_tag="LP543_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBL2320640.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16295..16912
                     /locus_tag="LP543_000449"
     CDS             16295..16912
                     /locus_tag="LP543_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBL2320641.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16930..17613
                     /locus_tag="LP543_000450"
     CDS             16930..17613
                     /locus_tag="LP543_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBL2320642.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18296..19579
                     /locus_tag="LP543_000451"
     CDS             18296..19579
                     /locus_tag="LP543_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL2320643.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            19595..20482
                     /locus_tag="LP543_000452"
     CDS             19595..20482
                     /locus_tag="LP543_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2320644.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20499..21335
                     /locus_tag="LP543_000453"
     CDS             20499..21335
                     /locus_tag="LP543_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2320645.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21338..21961
                     /locus_tag="LP543_000454"
     CDS             21338..21961
                     /locus_tag="LP543_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320646.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21958..23250
                     /locus_tag="LP543_000455"
     CDS             21958..23250
                     /locus_tag="LP543_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL2320647.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23609..23706
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23822..24403
                     /locus_tag="LP543_000456"
     CDS             23822..24403
                     /locus_tag="LP543_000456"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBL2320648.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24406..25713
                     /locus_tag="LP543_000457"
     CDS             24406..25713
                     /locus_tag="LP543_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBL2320649.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26129..26617
                     /gene="purE"
                     /locus_tag="LP543_000458"
     CDS             26129..26617
                     /gene="purE"
                     /locus_tag="LP543_000458"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBL2320650.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26610..27734
                     /gene="purK"
                     /locus_tag="LP543_000459"
     CDS             26610..27734
                     /gene="purK"
                     /locus_tag="LP543_000459"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBL2320651.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSIEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27747..29042
                     /gene="purB"
                     /locus_tag="LP543_000460"
     CDS             27747..29042
                     /gene="purB"
                     /locus_tag="LP543_000460"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBL2320652.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29222..29599)
                     /locus_tag="LP543_000461"
     CDS             complement(29222..29599)
                     /locus_tag="LP543_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320653.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29855..30583
                     /locus_tag="LP543_000462"
     CDS             29855..30583
                     /locus_tag="LP543_000462"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBL2320654.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30583..30837
                     /gene="purS"
                     /locus_tag="LP543_000463"
     CDS             30583..30837
                     /gene="purS"
                     /locus_tag="LP543_000463"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBL2320655.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30839..31510
                     /gene="purQ"
                     /locus_tag="LP543_000464"
     CDS             30839..31510
                     /gene="purQ"
                     /locus_tag="LP543_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBL2320656.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31511..33733
                     /gene="purL"
                     /locus_tag="LP543_000465"
     CDS             31511..33733
                     /gene="purL"
                     /locus_tag="LP543_000465"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBL2320657.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33718..35157
                     /locus_tag="LP543_000466"
     CDS             33718..35157
                     /locus_tag="LP543_000466"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBL2320658.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35180..36232
                     /locus_tag="LP543_000467"
     CDS             35180..36232
                     /locus_tag="LP543_000467"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBL2320659.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            36229..36807
                     /gene="purN"
                     /locus_tag="LP543_000468"
     CDS             36229..36807
                     /gene="purN"
                     /locus_tag="LP543_000468"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBL2320660.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36804..38339
                     /gene="purH"
                     /locus_tag="LP543_000469"
     CDS             36804..38339
                     /gene="purH"
                     /locus_tag="LP543_000469"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBL2320661.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38355..39629
                     /gene="purD"
                     /locus_tag="LP543_000470"
     CDS             38355..39629
                     /gene="purD"
                     /locus_tag="LP543_000470"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBL2320662.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39751..40665
                     /locus_tag="LP543_000471"
     CDS             39751..40665
                     /locus_tag="LP543_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL2320663.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40798..42363)
                     /locus_tag="LP543_000472"
     CDS             complement(40798..42363)
                     /locus_tag="LP543_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL2320664.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42356..43807)
                     /locus_tag="LP543_000473"
     CDS             complement(42356..43807)
                     /locus_tag="LP543_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBL2320665.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTIEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43821..44837)
                     /locus_tag="LP543_000474"
     CDS             complement(43821..44837)
                     /locus_tag="LP543_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL2320666.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45057..46145
                     /locus_tag="LP543_000475"
     CDS             45057..46145
                     /locus_tag="LP543_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HBL2320667.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46199..47725
                     /locus_tag="LP543_000476"
     CDS             46199..47725
                     /locus_tag="LP543_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBL2320668.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47727..48932
                     /locus_tag="LP543_000477"
     CDS             47727..48932
                     /locus_tag="LP543_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2320669.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49189..50166
                     /gene="guaC"
                     /locus_tag="LP543_000478"
     CDS             49189..50166
                     /gene="guaC"
                     /locus_tag="LP543_000478"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBL2320670.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50430..51854
                     /locus_tag="LP543_000479"
     CDS             50430..51854
                     /locus_tag="LP543_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBL2320671.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52315..53424
                     /gene="pdhA"
                     /locus_tag="LP543_000480"
     CDS             52315..53424
                     /gene="pdhA"
                     /locus_tag="LP543_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBL2320672.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53427..54404
                     /locus_tag="LP543_000481"
     CDS             53427..54404
                     /locus_tag="LP543_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBL2320673.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            54429..56072
                     /locus_tag="LP543_000482"
     CDS             54429..56072
                     /locus_tag="LP543_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBL2320674.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            56080..57486
                     /gene="lpdA"
                     /locus_tag="LP543_000483"
     CDS             56080..57486
                     /gene="lpdA"
                     /locus_tag="LP543_000483"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBL2320675.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            57648..57923
                     /locus_tag="LP543_000484"
     CDS             57648..57923
                     /locus_tag="LP543_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBL2320676.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            57929..58696
                     /locus_tag="LP543_000485"
     CDS             57929..58696
                     /locus_tag="LP543_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBL2320677.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            58864..60693
                     /gene="typA"
                     /locus_tag="LP543_000486"
     CDS             58864..60693
                     /gene="typA"
                     /locus_tag="LP543_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBL2320678.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            60954..62726
                     /gene="recQ"
                     /locus_tag="LP543_000487"
     CDS             60954..62726
                     /gene="recQ"
                     /locus_tag="LP543_000487"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBL2320679.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            62840..63142
                     /locus_tag="LP543_000488"
     CDS             62840..63142
                     /locus_tag="LP543_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBL2320680.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            63145..64308
                     /locus_tag="LP543_000489"
     CDS             63145..64308
                     /locus_tag="LP543_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL2320681.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            64334..67762
                     /locus_tag="LP543_000490"
     CDS             64334..67762
                     /locus_tag="LP543_000490"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBL2320682.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            67783..68907
                     /locus_tag="LP543_000491"
     CDS             67783..68907
                     /locus_tag="LP543_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320683.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            68897..69334
                     /locus_tag="LP543_000492"
     CDS             68897..69334
                     /locus_tag="LP543_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL2320684.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            69318..69656
                     /locus_tag="LP543_000493"
     CDS             69318..69656
                     /locus_tag="LP543_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBL2320685.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            69775..70335
                     /gene="rsmD"
                     /locus_tag="LP543_000494"
     CDS             69775..70335
                     /gene="rsmD"
                     /locus_tag="LP543_000494"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBL2320686.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            70332..70823
                     /gene="coaD"
                     /locus_tag="LP543_000495"
     CDS             70332..70823
                     /gene="coaD"
                     /locus_tag="LP543_000495"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBL2320687.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            70813..71856
                     /locus_tag="LP543_000496"
     CDS             70813..71856
                     /locus_tag="LP543_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBL2320688.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            72015..72743
                     /locus_tag="LP543_000497"
     CDS             72015..72743
                     /locus_tag="LP543_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBL2320689.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            72859..73104
                     /locus_tag="LP543_000498"
     CDS             72859..73104
                     /locus_tag="LP543_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL2320690.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            73101..73319
                     /locus_tag="LP543_000499"
     CDS             73101..73319
                     /locus_tag="LP543_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBL2320691.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            73455..73994
                     /locus_tag="LP543_000500"
     CDS             73455..73994
                     /locus_tag="LP543_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBL2320692.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            73987..76260
                     /locus_tag="LP543_000501"
     CDS             73987..76260
                     /locus_tag="LP543_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBL2320693.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            76298..77344
                     /gene="holA"
                     /locus_tag="LP543_000502"
     CDS             76298..77344
                     /gene="holA"
                     /locus_tag="LP543_000502"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBL2320694.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(77433..77687)
                     /locus_tag="LP543_000503"
     CDS             complement(77433..77687)
                     /locus_tag="LP543_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBL2320695.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(78087..78356)
                     /locus_tag="LP543_000504"
     CDS             complement(78087..78356)
                     /locus_tag="LP543_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320696.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(78826..79281)
                     /locus_tag="LP543_000505"
     CDS             complement(78826..79281)
                     /locus_tag="LP543_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBL2320697.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(79419..81347)
                     /locus_tag="LP543_000506"
     CDS             complement(79419..81347)
                     /locus_tag="LP543_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBL2320698.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        26435 a        14011 c        17658 g        23431 t
ORIGIN      
        1 tttttttaga catgaatatc ttatttttat tgctattttt cttaattttc cttctaacat
       61 ataggaaatg tcaaaactat tatgtgcgta tattgactgc attaccgttt ttcttaaacc
      121 tgattattta ttttggtaat acaatgggtc aaagctttac ttacgtcaat ggaaataaaa
      181 gggcaatgat ttggagtagt agtaacctga ataatctctt tacagagtta ggaacgaaac
      241 tgtcactttt ttatccaggt acttggatag ctactttagt agtcttggca ttattactat
      301 gtttaattgt tggaatttat ttaagttttg acaataagaa aacttctatt tttttagtta
      361 ttttgatgat aatgggattt tgttcaaggt taattatggg attttctccc acagtatggg
      421 cttccggaat gcggacgtac tatatacttt atgttgttat cgccatctta gtactaatgg
      481 cagttaaaga gctaatgaaa agcatgagtg tacaaaaaaa tgaatttatg caatttggat
      541 taaccgtgct tggtatttgt acctttataa taacggtgat aaacagataa tatacaaaaa
      601 atccctcgct ttagtattag gtgagggatt ttactcgttt ttcgattaat tgttttaaca
      661 cttgcaagtt tctacgagta gcttgtttcc gaccttcata tactcacctc aggctgttta
      721 catgattgta cgttcttttt gtaaaggata cagtgatatg tatataatgg cataaagaaa
      781 cgttcaaaca aaaaaactcg tgaagcaaaa ttaacttcac gagttgtagt gcagatgatg
      841 ggactcgaac ccacacgagc taacgctcac atgaccctca atcatgcttg tctgccaatt
      901 ccaacacatc tgcgtaataa taacacacta atgatagcga ggttttacat agttgtcaag
      961 ctcaaaaatg ggaggactta ttaagatgga gaaatatgac ggagaatttt caggactggg
     1021 aatgatctta ggtgtattga ttggacttgc attcggacgt tttttgcttg gttttatgct
     1081 aggaatcatt tgtggaattg caatggattg ggcagcgaat ctatggaatg actatcatga
     1141 caattaagtt ctcaacgctt gtttgtgttt ttttaatact tacgtttttc ctaaataagt
     1201 ggcaaaattt tctaggatgg agtatgatat tgaaatagta aataacaaaa aatttggaaa
     1261 gttctaactt tttgaaatga gggattggct aagtgatgag agaatttgaa aaatccaaca
     1321 aattagaagg tgtcagttac gatgttcgcg gtcccgttct agaagaagcg gagcggatgc
     1381 aagaagaggg cattagtatt ttaaaattga acacgggaaa tcccgcacct tttggatttg
     1441 aagcaccaaa cgaagtcatt cgtgatttga tcatgaatgc ccgtgactca gaaggctact
     1501 cagattccaa ggggatcttt tctgctagaa aagcaattga gcaatattgc caactgaaac
     1561 attttccgaa tgtgacgatc aatgatatct acaccggcaa cggagtgagc gaattgatca
     1621 cgatgtgtat gcaggggttg ctggacaatg gagatgaagt actggttcca atgccggact
     1681 atccgttgtg gacggcttct attgcattag ctggaggcac acctgttcat tatatttgcg
     1741 atgaagaggc agaatggtat cctgatattg atgatatcaa atcaaaaata acgagtcgga
     1801 caaaagctat tgttatcatt aatcctaata atcctactgg ggcactttat ccaaaagaat
     1861 tattagaaga aatcgttgaa gtagctcgac aaaataacct gattatttat tccgacgaaa
     1921 tctatgatcg cttagtgatg gatggtctag aacatattcc gattgctaca ttagcgcctg
     1981 atttgttcgt tgttacgctg aacggtctat ccaaatctca tcgtgtagca ggatttcgag
     2041 taggctggat ggtcctaagt ggagataagt cacacgtcaa aggctatata gaagggctga
     2101 atatgttgtc ctccatgcgt ttgtgttcca atgtattgtc tcagcaaatt atccaaaccg
     2161 cgctaggcgg ttatcaaagt gtcgatgagc tgctcctgcc aggcggaagg atctatgaac
     2221 aaagagaata tatctataat gcaatcaatg atattcctgg tctttcagcc gtaaaaccaa
     2281 aagctgcttt ttatatattc ccaaaaattg atacaaagcg attcaatatt ttggatgatg
     2341 aaaaattcgt tttggacttt ttacatgaac atcatattct acttgtacat ggtggtggat
     2401 ttaattggaa ccagccagat cattttcgaa tcgtttatct tcctaaaatg gatgatttga
     2461 aacaaacagc taagaaaatg agagaattcc tagcaaccta tcgccaaaaa tgattttaaa
     2521 aaccaaattt ttcagtagga gggccacaga caaacacact gaggctctct ttctagtgga
     2581 gttttcttaa ataactaaag tatattattt agttgcaagt cttttcttga agtttgcgac
     2641 ttcctaaagt aaaatacgaa tagaatgcga atacgcatat tttggggagg atacttatgg
     2701 aaacgttaat cgagtccttt ggaacgatca ttaaagagat tcgaaaagaa caaaaaatga
     2761 cgcaaaaaat gctttcgcat gatatttgtt cacaaagtgt attaagtcgg atcgaaaata
     2821 atgaggaact gccgaatgta ttggtgatga tgcagatttg tcaacggcta ggggtaacga
     2881 ttgatcatgt gatgaatctc tctcgaaaaa ctactcggac aaacgataaa ttatttgaac
     2941 tgttggatag ttgtttccaa aaacgtgatt atcggaagtt agaacaagta ctaaaatctt
     3001 ctgatatttc tgaaaatctg tatcaagcaa ctgactttca acgctattat tattatctcg
     3061 gtgtttgcga attcacgttg cgtcaaaata tctctcagtc cctttattat ttgaaagaag
     3121 cattaagcta ctctagccaa aaagaaagaa tccatgtttc tgacacagaa atacaactga
     3181 ttagttgtat cggaaaaata tatggtgtgg caggaaaaac ggctgaagca cgctactatt
     3241 tgagtcgaag catccaactg ctgcatcaat cagctgatga acgatcaaaa agtgaatttt
     3301 cgcaaatttt ttataattat ggaaatttct tattccatca aaatgaaatc gacgaagcgt
     3361 tagaacaggt gaatcaagga atctattggg ctcaagagaa gaatagctac tattacctca
     3421 atgatttatt tgtgcttaaa agcttaatat acaagcgtaa agaagaacca gaaagagcag
     3481 cattctatga aggcttagca caagctgtga aacaaatttc aaaaaatttg taaatggtgt
     3541 agaccattct tgacttcttc tctaaataag agtactttag aggtagaaaa acaaagggag
     3601 agaatagaat gagaactttt atctatgctg acaaattctt tttagcgtca ggtgtcaaac
     3661 atgcaggcta tcttgaaatt accgatggac tttttggtga atacaccaaa gaactacctg
     3721 agggagaagt caacatcatt gaccaaagcg gcaaatggat tgctcctggt ttggtagata
     3781 cacatattca cggctatatg aatcatgatg taatggataa tgatgcagaa ggcatcaaag
     3841 tgatgtctga aggtctgtta tcttgtggag tgacttcatt tttaccaaca acattgacgt
     3901 caagcaaaga acgtttaaaa gatgtagctg agacaatcgg caaaatgtat caggaagcac
     3961 caggagctaa aatcaaagga atctattttg aaggtccttt cttcacagaa gaacacaaag
     4021 gggcacaaaa cccaagttat ttcggggatc ctgatttgga tactttccat gaatggcaag
     4081 aagcttcagg tggtttaatc aaaaaaattg ctttagcacc ggaacgtcaa ggtgtagaag
     4141 agtttgtcaa acaagtgact gaagaaggag tagttgtttc tctaggacac agtaatgcta
     4201 cactagaaga agcgcaaaaa gctgttgaag caggcgcaag tgtatttgtc catgctttca
     4261 atggtatgcg tggattgaat catcgcgaac caggtatggt gggtgcgtta ttatctttag
     4321 aacatgtctt ttctgaattg atttgtgatg gccatcatgt acatccaaat gctgcgggaa
     4381 tcctaatgga aaaagcagga catgatcatg tcgcattaat tacagactgt atgatggccg
     4441 gtgggatgcc tgatggaaat tacaatttag gcgaatttcc agtagtagta aaagaaggaa
     4501 cagcacgtct agaatctggc aacttagctg gaagtatatt gaaattgaaa gaagcaatta
     4561 aaaatgttgt agactgggga ttagcaacac cagaacaagc agtcatgatg gctagtctga
     4621 ttccagcaat cagctgtaaa atcgatgata catgtggaaa aatcgctcaa ggacgcgatg
     4681 cagactttat cgtattgaac cctgatatga cattggaagc aacttatcta gatggcgtgg
     4741 aacgtttcca cgcataatat aatttaagtt aaccttaaaa ctaaacttct taagtttggt
     4801 tttaaggttt tttttgtgtt ctagtaaaaa tcactttaca taagtttcaa aagtttcttt
     4861 aatttcctat tgttcaataa gtaacaatgt aaagataaaa ttaaaaatgt taatacgtat
     4921 tagcaaaata aaagcttttt ttattgtgaa taaatgaaca taattaggag gagaaagatg
     4981 aacttatttg acatttttcg atcgactttg acagatatgt cgattgtcag tgcaatcact
     5041 tcaacagttt ttattatatt attagggttt gtttgtagaa aaaaggggat tttttctgaa
     5101 tctgttggaa aaattctatc gaaagtcgtc ttaacagtgg ctttgccagc actggcattt
     5161 cgagctttca tgcaagatat caatacagaa ttactgatgc aaggaatcaa tgtcttgatt
     5221 tggggattag ttatttatat tattcttatt ctagtttcaa aacctttttt cttaagctat
     5281 aaaggggata agcaaacaac gttgcgtgtc ctgacgatct ttggttctac gacctttttc
     5341 ggaacgccta tcgtcagtgc gatttatggt cctgtcggcg tgatgtattc ctcaattttc
     5401 aatattggct accgaatctt tttgtattct tatggataca taaaaatgag tggcttgaag
     5461 atggaagcta aaaatatcaa agcgatgttc gtcaatccaa tcattatcgc tacttttgcc
     5521 ggccttttca tttggctgtt ccaagggtat ctgccacaag tgtcagttac aggtgcagaa
     5581 ggagagataa gccaagtagc gtttttgcga attgaccaga ctgcaccgtg gctatttcag
     5641 ccgatggatt atttagctgg tttaagttca ccattggcgt ggctagcaat cggcgcaaca
     5701 ttaggtgaag taagcttcaa aaaagctgca tctgaaaaaa cttcatggta ttacagtcta
     5761 gtcaaagtgg tatttgtacc tgcattgaat atcgtgattc tagcagtttt gacggcaacg
     5821 aatattctac cagtcaattt tgaagcactg gcaacaatcg ttatcatgat ggctacacca
     5881 gctgcaacag tggcagcagc ctatgctatc agttttgata gggaggcact tttagcctcc
     5941 aatgcatcct tactgtcaac tatttttgct gtggtactga cacctgtatg gatcgttgta
     6001 ttacaaatga ttcatggcat aggtttattt tcataagaaa tggaggaaga aagtatgaga
     6061 atcgcatgtt acggtgttcg tccgaacgag atagattttt ttgaaaaatt gaatatttac
     6121 cattatgatc tcagtcttta tgaagaactg ttgacacatg acaatatcgt gtcagcaaag
     6181 gaccatgatg ctgtattgtt gaggggaaat tgtatagcag atgagaccaa tcttgcaaaa
     6241 atgcaggaat atgggattcg ttatgttttt acacggactg tgggagtcaa tcatatcgat
     6301 ctacaagcgg cagctgattt tggtatgaca gtagcccgtg taccatcgta ttcgccaaat
     6361 gcgatagcag agttatcttt gacttttgcc atgatgcttt tgagaaatac tgcgtatacg
     6421 acgatccgaa catctttcaa agattttcga gtggatgagc aaatgtttag tcgtgaaatc
     6481 agaaattgca cagtagggat cataggaaca ggcaggatag ggttgacgga agctgttttg
     6541 tttaagggac taggagccaa agtgatcggc tacgatattc atcagacaga agctgctaaa
     6601 aaagtattga cgttccactc gcttgaagag ttattagcca aaagcgatat tgtcagtatt
     6661 catgtacctc atatcccagg tgaaaacgat caaatgatca atgaaggatt tttagctcaa
     6721 atgaaaaaag gtagcatctt gattaataca gcccgtggag agcttcaaga taatgaagct
     6781 attttaagag ctttgaaaag caatcattta gctggttttg caaccgatgt ttttgcaaat
     6841 gaaacagagg tttttttccg atccttcaag ccatgggaga cgattccaga ccctgctatc
     6901 cagcaattag tagaaatgta ccctagagtt cttgtaaccc cgcatgtagg ttcaaataca
     6961 gatgaggcat tatcaaatat gatttcgaca agtttcgaga actttcgtga aataatagaa
     7021 actggaaaaa cgaaaaatga ggtatctctt cctaaagccc gtcaattaag ataagaaaac
     7081 atggatattc gtcaataaaa tgtttaaaaa tagtccaagt aaacagttgt tacttctgta
     7141 tacttggact attttgtatg ggataattcg atgactcaac agtaatctgt gagctttttc
     7201 cgcccattct ttgtcaattc ataacaggtt agcggccggc cgatttccaa atatttgatt
     7261 ctttcgttca atatatcttg ttctactaaa aaattcaaat atttcttgac ggatatacgt
     7321 gacagtgagc ttttttgagc cagttctttt gttgaaaaaa tagtatcttc ttttagaata
     7381 aatgcactta ttttttttaa agtcagtttt gacagacctt taggcaaatc gatgaatgat
     7441 ggtatttcgt gaatgggagt ggtgtggttg aatacttgat cgatcaattt ctgatcggct
     7501 ttttcagtcg tttggattgt ctgagagaaa gcaagatact tttcgatcgc cagctgaaaa
     7561 cgttgcaaag tgaacggttt gatcaaataa tctaaggcac cgtagcggat cacattcttt
     7621 atggtttcga tatcatctgc agcagtgatc aaaatcactg ggatatcatt tccttgctgt
     7681 ctaagccaag ataaaaaatg gataccactt tcgccaggca tgtatacatc aagcagcagc
     7741 agatctgcgt ttttttgtct gaaatatttt tttccttctt ctacatttct gaaactttcg
     7801 attacgttca tattagggat gctttccaca aaagtacgat tcaattctgc aaccattgga
     7861 tcatcttcga ttatcaggac atttgtcatc attttgatct tccttttttg gtaattctag
     7921 atagacgatt gtaccgccat ttgtttgttt ggaaaattct aaaaatccac cgtggcgttt
     7981 gacgattgaa tggatgagat tcaatccaaa cccacgattg attcccttcg tcgaaaaacc
     8041 tagcgtaaat attttttctg agatttcttc tgatatcccg acaccgtcat cgcagacggt
     8101 cacgatgaat acttcagctt ctgtgtcata gtttaaatga atccggacct gtccgtttct
     8161 ttcatgtttt tgtagcacgg cttcttttgc attatcaagt aagttcccta acacttgcaa
     8221 aagtgaatgg atggagtcat ccatctccat tttagggata aaagaatcgc tttctaacag
     8281 catttcaaca gattgttctt tggcttcgtt tattttgcct aataaaaaac cggcaattgc
     8341 aggtactttg atcagacttg agacttggcc aatctctttt ttataatttt gatttagctg
     8401 ctggataaaa tgggtgactt cttcatactt cttcaaatca atcaaaccag aaatgacatg
     8461 aagtttattc atgaattcat gggtttgagc tctcatggag tttatatatt gtttcgtccc
     8521 cccaagctcc tccattaatt tcattacttc tgatcgattt cgtaaagttg cgactgctcc
     8581 taaaaaacgg tcattgctaa aaagaggtgt aacgctagaa atagtttcta tcccatttaa
     8641 atacatggat tgagtccgaa cggtcgtttg atgctcaatc gtctctttaa acaaaacgtc
     8701 atatagatta tcactgattt ttttcctact tcgaaatcca atagctgctc tgtttggtag
     8761 aaaatgtttt ttgcaggtgt attgatcaag aggatagttt tatcagaaga gatggctaag
     8821 attccttcat ctacttcatt ttcgataatc gttttttcac ttaactgttc tgctatttct
     8881 tgtggttcaa gtccaagcag tacttttttc attttttggg caagaaaaac ggcgcctatg
     8941 atgcctacgc taagtccaat aatcaatgaa aaaacaatca ttttttgtcc atctttgact
     9001 tgattgtcaa tcgtgttgtt tgttaagcca acacaaactg caccaataat ctcttgatca
     9061 taataaatag ggacgaaata gcggaagccg aaccctaaca ttccttcttt ttcagagaaa
     9121 aattcttgtc ctccaatcac ttttttcaca tcttcatgac ttgagaaagg aagaccgatg
     9181 taacttgggt ctgcgtgaga atatcgaatg ccttcagggt ccatgacaac gataaaatct
     9241 aaattcgatg caatcatgat ttcatttgtt attccttgaa tcgcatgtgt cgggtcacca
     9301 gtctgcagct ttttttggat gagtttatca ttggcaacga ttttagcaat gctagagatt
     9361 ttatctgaca cactagatcg gttcatcgaa agaataaaat tcctgatcaa tatggcagaa
     9421 acaatcaaag aaacgaatgt ggagaaacaa acgacaagga tgatcgtgtt ctggagcttt
     9481 aatggttttt tcttaaaaaa aggaaacata ggctacctcg aaagaattta ttgtgaaaat
     9541 tttaacatga aaacaaaaat ttaacaaatt gtcaacgata aacacaacct tcctataaaa
     9601 aacaccactg agtcggtaaa agaagctata cagaattttc aaacattggt atgatagacg
     9661 tagaaatgga aggaggcgtg tatatgatag ggggaatcgc acttgagacg atttattggt
     9721 atacattgct gatttgtgca ggattggcag tcctattgat tatttttgga gatgtattcg
     9781 attttgatgg cccgcttgat ccgatgctta ttatcccttg gttaactttc acaagcttat
     9841 ttggttttct aggtgagcgt ttaacagatc aatcatcttt atttttattt attgctagtg
     9901 gaggcatatc gatagtgttg gtatttttgc ttaatttcta tatattgatg ccgatgaaac
     9961 atgccgaatc aacgatgtcg tcttctgaaa aaactctgga aggacaagta gctgttgtga
    10021 tcacaccgat ccctgtacga ggtatgggag aaattcagtt gaaaagtgtt accggttcca
    10081 ttagtcgtcc agcttgtttt tacagccccc aagaacagac agctgagcgg ggaacgaaag
    10141 tattgatcat tgagatcaag gaaagaatct gttatgtcat gccttatgaa agcatgataa
    10201 aagcttaaag gagcgtgtgt taaatggaaa taccagttgt ttttatcgcg atcgcaatcg
    10261 cagctttgat tttactgatg ttgctgatcg tctttgtagc gaagtaccag acagcaaaac
    10321 cggatgaagc gttgatcatc agtggaagtt atttaggaag caaaaatgtg cacattgatg
    10381 aaagcaataa caaaatcaaa attgtgcgtg gaggtggcgc attcgtcttg ccagtttttc
    10441 aacgttcgaa tcggatcagt ctattatcga gtaaattgga cgtctccacc cctgaagtgt
    10501 atacggaaca aggcgtaccg gtgatgtgtg atggtacttc gatcatcaaa atcggttcat
    10561 ctgtagaaga gattgctaca gcagcagaac agtttttagg caagacaaga gaagaactgg
    10621 aaaatgaagc aagagaagta ttagaaggac acttgcgctc gattcttggt tctatgactg
    10681 tagaagaaat ctatcaaaat cgcgataagt tcagtcaaag cgtacaagaa gtcgcttctg
    10741 tagaccttgc aaaaatgggg ttgatcatcg tttctttcac tatcaaagag gtcagagaca
    10801 aaaacggtta tcttgactct ttagggaaac caagaatcgc ccaagtcaaa agagatgcag
    10861 atatagcaga agcggaagct ctaaaagaaa cgcgtatcaa aaaagctcaa gcagaaaaag
    10921 aatcacaagc agctgaatta cagcgacaaa cagagatcgc agaatcatta aaagaaaaag
    10981 aactgaaatt agcaacctac aaacaagaac aagatgttgc taaagcaaaa gcagaccaag
    11041 cctacaattt agaaagtgcc cgagcacaac aacaagtaat cgaacaggaa atgcagatta
    11101 aaatcgtaga acgtcaaaaa caaatcgaac tggaagaaaa agaaatcaca cgtcgtgaaa
    11161 agcaatatga ttcggaagtg aagaaaaaag cagatgccga tcggtacgct aaagaacaag
    11221 aagcacaagc tcaaaaagtc aaagaagtga ctgaagccga agcagaacga ttccgtgtgg
    11281 aagcactggc agaagcggaa gccaataaga cacgtttagc tggacaagca gaagccgaag
    11341 cagcactagc aaaaggaaaa gcagaagccg aagcaaaaca aaagatcgcc aatgccttca
    11401 aggaatatgg cgaagcagct gtcttgagta tggttatcga tatgctaccg caattgatgc
    11461 gcgaagcagc acagccgcta ggaaatatcg aaaaaatctc tgttgtcgat acaggaagca
    11521 gttcaggaga aaccagtgga gcgaaccgag tgacaaatta tgccacaaat ttgctttctt
    11581 ctactcaaga aacactaaaa gcgacaacag gacttgactt gaaggaaatc gtagagaatt
    11641 tgtcgacacg tgggactact agccaagtaa atatgtatga agcgccaaaa gaggcagaac
    11701 aaaactcgat gaaggaagaa taaagtagta cacttcaccg ttcaatcaaa aatggaggaa
    11761 gactttttaa agttcttctc tccatttttt tattttattg caaaagaaaa ttagtcctcg
    11821 ctatttttct tttgtttttg gatcttagct tctatctctt gtaaatttgt tcttagcaat
    11881 cgttctttct tttggagttc gaccaaatct tctgatagtt cttccttctt ttctgtttca
    11941 ggaggacgaa gaaagaaatt gataccgatt gcaatgaatg tccctataaa agtatctagt
    12001 acacgttgga tagcatacaa agcggattcg ccttgaggga tgctcaacgc aatcagcaac
    12061 aacgtagcta ttgcggaaat gataccggaa ttattgttaa taccatctga cacgacgatc
    12121 acaataataa ctagaatagg aagcaagatc aattctacca aaaaatcatt gtgaaaatag
    12181 cttttgacaa aaaaataaat gatagctaat gtaccaccta atgtattgcc gatgatccta
    12241 gactttccaa aagaaacggt tgtagtcaag tcctgacgca aagaaaaaac agcagataat
    12301 gcagcaatca tcgggattcc tcgttgagtc aaatgaaaaa acaagataca taaaaaaaca
    12361 gctaaggcac ttttgatcgt acgcatacct agtcgaaaac ggccaaattg catagagatt
    12421 ctcctttttg gtctttttac tatcatacct aaacttttgt ctgatagcta gtttttcatt
    12481 attcaagtat aattaaaata aaaaaggggg aagtaaatgc gtataggaat gcgaacagtc
    12541 aaaacagtga tcagtgtctt cttttcgatg gtcacagcgg gtagcttatc gctgttgtat
    12601 tggccagctg cagggatcat tgcattatta agtgtaggaa ataccaagcg ctctacattg
    12661 cgtacgggca tgaatcgaat cgcagccttt atattagcta cggcaacagc actgttcagt
    12721 ttttttgctg ttgggtatac tatttttggc ttcagtcttt ttttattcct gttcatcccg
    12781 cttgcctctc gcttcaaatt aaccgaaggg atagtagtaa attcggtttt ggtcactcat
    12841 tatttagtgg aggaaaacat gtcttggcag ctgatcggca atgaagcggg attaatggcg
    12901 attggattag tatttgcttt gcttgcaaat gtttacatgc cagatcgtac gaagcagctg
    12961 aaggaaaacc aaatacagat cgaaaaagaa tttcgaaata ttttgagaca aatggcagaa
    13021 tttcttttat cagaaaataa agaagatgtg cagatcaaat gcgaacattt actgacattc
    13081 attcgcgaaa gccaagaaga tgccagagaa catcaagaaa actactggct cagacagccg
    13141 ctatactacg aaacttactt ctctatgaga agagcacagg ccaatgtgat taaagacatg
    13201 ctggaaaatc tagagagaat ccagcaacca gcttattatg gaaaacatat ctatggttta
    13261 ttgatttata ctgcagaaac attttcagag tcaaatgatg gcagacaaat tttaacacgt
    13321 attgaagaag tttatgtatt gtatcggcaa atgcctctgc cgactagtcg ccctgaattc
    13381 gaggatcgag cagaattgtt ccagtttttg cagtctttta aaagttttat tgaaatcaag
    13441 gcagaattct cccaacaaaa aatccaaaaa tagaggttgt gaattcagct aatattcagg
    13501 tcaaaaagct gatttcacaa cctcttcttt aaacggtgtt ctcctgtcaa ctcctcggtc
    13561 acaagtcaca atattcaaca aacatgagaa gctgtttttt gaaattgctc cttgtgactc
    13621 ggagctgaac ggtaggctca caacctcttt cttaaacggt gttcaaaagc tttcctctgc
    13681 ggtaataagt ccagccaaac gaaaaatagg aggagctatt tttgtttggc tgttcttatt
    13741 acttgatgca gaacagcttt ttcacaacct tttaaaatta catatttttc catagaactt
    13801 gcatttgatc caagatttcg atgacatctt ttgtcaagaa gagagagcga ttgggcaagt
    13861 caccttcaat catgtgtacc atattcttta tttcgtaatt cattgcttca gatgtattcc
    13921 cgctttcgat gaattccttt gtaccatctg taaataagat ctccgcacga tcagcacgcg
    13981 ggtaatcagc aatcgtcaca taaccgttct caaaggcaac gataccgact tttggcattt
    14041 ttgcctgaaa agtcaagctg acagcagcaa gttcattttc tttgttacgc aagatagtga
    14101 cggattgttc gtccacgcct gtttcaaaag gaaccattgt agaggcaact acttcaggtt
    14161 gagagctcaa aaagaaacga gcgaaagata cagcatacgt accgatatcc aacagagctc
    14221 ctccagctaa ttctggattg aagaaccggt ttttagggtc tggttctttg taactgccga
    14281 aaggtgcttg gatcattttt aaagccccca gttttcctgt atccattatt gagcgtaact
    14341 gctgataaag tggcatgttg aagattgtca tcgcttctgc taagatcaaa ttcttttctt
    14401 ctgctaagcg catagcgtca gccagttctt ttttattcat cgtgatggct ttttcacata
    14461 gaacgtgttt gcctgcttcc aatgctgcca agatatggtc gatatgctgt ctatttggta
    14521 cagcaatgta cacgatgtcg atttcagcat cgtctaacat ttcttgatag cttccgtaag
    14581 cttttggaat attgtaacgg tgagcgaagt tttccgcttt ttctgaagtt ctggaagcaa
    14641 ctgctgttag ttcgctcgtc tcttgattga agctttcggc aaactcatgt gcgatggtac
    14701 caagaccaac gattccccat tgatattttt tcatctgaaa acgctccttc atagttggat
    14761 tgagtttctt gtctgtctat tattataaca aaaatcacga agaataaaga ggagaggact
    14821 tgaaacatta aaaaaaatga aagcagcgat ttaagaggtg ctttttgatc aaaacttaag
    14881 tagaatagtt aaaaggaggg gataaacgtg catcaagtat atattgatct tattatgagt
    14941 gtattattag aacagtttga aactgaaaaa aattttttag atgaatactt gctgatcagt
    15001 ccagaacaat ggcaaaaatg gaaagatggc gagaaaaatc tgccatctga agcgatgcag
    15061 aaagtaaaaa atcttttcac tgattatgaa tggatgctaa cgcaaaagat tttgcgtcaa
    15121 acgattattt ttcccgaaaa acgaaacgtt gccgttgcag agtacaagca aatcaaaaca
    15181 cgcatcgcgc aaaaatggct tagcagcaaa gcagcaaaag tcgagctgat ccctttgaaa
    15241 aataatcagc aggctggtag ataccttgac ttgaaagtaa gtattcaata tgatgaatgg
    15301 ggctatgatg atattttgaa ttttcgtctg ccagcagatg ttcaaaaaca gatcgaagga
    15361 gaaaaaatcg aacttctcga ttgggtgaat gaaaatctgg aagaaactta cgttcatgag
    15421 gcgaatgggg aacgtgaaga acaacgaaag gatttttcaa gtgaataaat ttttaggatt
    15481 tggagcagtt gtgattatga tcgcagcaat gggctatgtt atttttagcg atgatgattc
    15541 gcatgttcaa gccggcaatc aagcagtcga acgaacaagc accaaaacaa ctgctagtaa
    15601 agaaaagaac agtcaaacaa ccagcagcaa ggtaaaggac aaagaaacaa aagatcgtac
    15661 gtccaaacaa ttgccagatg ttaaaacaga cgattggaag ctagttttgg ttggtccaga
    15721 tcacaaatta gaaaaagaaa ttgatgagtc tactcaattg gcatcattaa gtaacggata
    15781 cttgatcgac aaacgcattg ctccagaata tgaagcattt gccaaggctg ctgaagcagc
    15841 tggatttcca ttagtgatgg tctcagctta ccggtctgta tcatcccaac aacaagtttt
    15901 cgaacaaaat gtccaagatg tgatgagtcg tcaaggtgta tcagaagagg aggcgacaaa
    15961 gattactaaa gaaacgatta cggagccagg atacagcgaa caccacacag gtctggcagt
    16021 cgatgtcgtc gatcaaaact ggtacaacag ttatccttct actgtattgg atgcaagtta
    16081 cggagatcag cctggagcta aatggatcgc agataatgct gcgcagtatg gatttatcgt
    16141 tcgttatcca aaaggacacg aagatataac taagatcaca tatgaacctt ggcacttacg
    16201 ttatgtcggt aaagaaaacg ctgagtatat caccaaacac caattgacgc tagaagaata
    16261 tctgaatgaa ttaaatgaaa agtaggaatt gatcatggcg aaaaaaaagt ggaaaaagca
    16321 aaagcgcaag aatatccgtt ggccagcagt ggttgccggt gtagcagtga tactcttagc
    16381 ctttgttttt tccttgaaga atctgtcttc tccttttact gataatcagc aagaaaatga
    16441 agcgcaatcc catcagcaat ttattgaacg gcttgttccg catgcccaag aactacaaga
    16501 tggttatgga gtgcttccta gtatcatttt aggacaagct atattagaat caaattgggg
    16561 aaaaagtacc ttggctagca aatacaataa tcttttcggg atcaaggctt atggggatca
    16621 gaagaaagta agcctggaaa caaaggaatt tgttaacgaa gaatggataa ctatccaagg
    16681 agacttcaaa gtgtatgatt catgggaaca gtccatggat gaccatacac aactgttcgt
    16741 ccaaggagta gattggaatc ctgcgctgta tgaaaaagtg attacggcca ccaactatca
    16801 ggaagccgca caagcattgc aagatgcagg ttatgcgaca gaccccggtt atgcgcaaaa
    16861 aatcatccag gtcatcgaga cgtatcaatt gaatcagtat gaccggagat agcaaaggag
    16921 aagtggctga tggcagaaca atttacgcca gaaatcatga cggaattgag aaaagatatc
    16981 gtaagagtac cggaaatcat tttagaagca agcgggatca agatatttgg aaaattgatc
    17041 cgttcgatca tctttactac ggatattgcg atcatacgta atacaaatgc gaatgcagta
    17101 attgctgtgt atccttttac acctcatcca gcgattacta aaagcattat tgaggcagct
    17161 gacattccag ttttctccgg tgtaggtggt ggtttgacac aaggtgctcg ttcttcctac
    17221 atgagcatgt tcgcagaagc gcaaggttcg ttaggggtcg ttttgaacgg tcctacgcct
    17281 ttagagacag taaaagaagt ttgtcgagta gttgatattc cagttatcag tacggtaacg
    17341 tcgaaatata cagaaataga tgaaaagtta gcagctggta tttcgattat caatatcagt
    17401 gcaggtaaag aaactgcaga agcagtacgc cattttcgta aaaaatatcc tgaacttcct
    17461 atcattgcga caggtggtcc aacggaagaa agtattgctg agaccattca agcaggagca
    17521 aatgccatca catatacgcc accaagtagc ggagagcttt ttagtcgtaa aatggaaaaa
    17581 tatcgggaaa ctgagaaaaa gaatcatgaa taacaggtga gaatcaaaag acgaggaaga
    17641 gcagatagtg gctcttcctc gtcttttgta ctccagtact atttgttcag aattcaaaaa
    17701 aaagagaaat ttttttgttt ttttacctgt aatgtacata agaagagtta aaaccttttg
    17761 ttattattag cattctaaca aaaaaatgtc ttgatttatt gaagcgcttt ctatagaatg
    17821 aaaaacgaat aaaaactaaa aaaggcaccc gacaatttag tagcagctaa attgtcgggg
    17881 cttgaaaagt cagcgggaac tgaccaatca agttgccata gtcaatgctt gtgcatcgac
    17941 gtgtatattg tacctaattt catacagaat tactagttat ttttctgttt tttagggaag
    18001 agcgcacacg aagaagcatg cttatttagc tatgtcaagg atattgggca gttgtctgat
    18061 atcctttttt ctttgcgtaa ttatcctccc atcattgaag caagaagtgt ttttagcgat
    18121 gaaagaagaa tcagcactat ctaatttttc ttgaaagtat tgagttgttc atcctaggca
    18181 gaacgataag gcaagtcttg gtttttattc cccttgaaaa agatcgttta ataaatatgt
    18241 tctgtataaa aatacagtat attgaaaaat aacaatggaa gtagaggtgc agagaatgaa
    18301 gaaaagaatg agaatcatct tagctttaag tgttatgtta ttcttcttga cggcttgtgg
    18361 gcaagaagat gacaaaaaaa tcacgctgaa catggcttgg tggggttcgc aagtccgaca
    18421 cgatgcaaca gtaaaagtaa tcgagcttta tgaagaaaaa aatccccatg tgacgatcga
    18481 ttatgaattt tatgattatg aaggatatta cacaaaatta ggtacactag cagcatcaga
    18541 tgatatctgg gacattttcc aactgaacaa tcaatttcct caatacatca atcagattga
    18601 accattagat gaatatgtgg agcaagggat aattgatatt tctaatgcag atgaatccta
    18661 tttagcaacg acgacttacg acgggcaatt agttggactg tctaacgggg tgaattcgtt
    18721 tgcgattgct tacaataaaa atatatttga acaattaaat atccctgaac cagcttcaaa
    18781 ttggacatgg gatgaatttg aacaaatcag ctatcaaatc actgaagaat taggaatcta
    18841 tgctatttca agattcgaag attttatagc tggctgtatt attcaaatac cacaagttga
    18901 gaaaggattg aatttttata atcgggaaga tctatctaac agcttgggat ttgaaaatcc
    18961 agactatcta acagattttt tccagctgcg taaaaatctg gtagacaaag gggcccatcc
    19021 agaaccaggt ggtggagctg caagtacgac ggatctgtct tttcaggcgg ttagggatag
    19081 agaagcggca atggtgtttc tatcaagtaa ccagttgaca gaaatattgt caggggctcc
    19141 tgaagatatg gagattcagt tgattaatcc acctcgcaga aaagcagatg gagaatctgg
    19201 tatcccttta cgttcttcgc aaatgctgag tatggcaaaa agctcagccc ataaagaaga
    19261 agctgctaag tttattgatt tctttcaaaa cagtgaagaa gccaatgaaa ttttaaaagg
    19321 agaacgtggt gtaccaatca tgtcgaatat tcgagaacag ctcgctcgtg cagatgatgc
    19381 aactttgcaa gcgacatttg attatttaga aatgatttcc gatatggaca acggcgaagt
    19441 gaatgtcttt gatagtgctt tgaatccgga aattcaagac caatacaatc ttttagttga
    19501 aaaagtcatt tatgaagaag taacagcaga agaagcagca gagagtcttt atgagtttgc
    19561 gtcaaacatt ctaggatagc agcaggagga aggcatgaaa aaagaaaaac taaaacggtt
    19621 cttggatcaa gacaatgtgg tcggttacgt atttgcttct ccattcatcc taggatttct
    19681 attgataaca gtgataccga tgatcttgtc actttattat tcgttcaccg attatcgttt
    19741 gggaaatcca gtctcatgga tcggcttgga taattatata aagatgctga gtgatgcccg
    19801 ttttttgaac tccatccgag tcacttttct ctatgtattt gcatctgtac ctgtgaaact
    19861 gatctttggt ctattgattg cttatctttt aacgaggaat atcaaaggaa cgactttctt
    19921 tcgttctttg tattatgtgc ctagtttgat cggtggcagt atttctatcg ctctagtttg
    19981 gcgggagttg ttttctaaaa acgggttagt gaatattttc ctggaacgat taggcgcacc
    20041 tgcgctttcc tggtttagcg atcaaaaact ggtcatgatc cctcttgttt tgatgtctgc
    20101 ttggcaattc ggttcggcaa tgatcatctt tgctgcaggt atcaaagaga tcccaggctc
    20161 gtattatgaa gcggcagaga tcgatggagc gaacaaatgg cagacgtttt acaagatcac
    20221 gttacctctt ctttcgccga tcattttgta taacttggtt atgcagacaa tcgcggcgtt
    20281 tatgactttt acgcaggcat ttatcattac ccaaggtggt ccgaatgatg ccacgaactt
    20341 ttatgctctt tatgtgtata accaagcttt ttcttttaat tctatggggt atgctagtgc
    20401 tatttcgtgg atcatgctgt tgattatggg agctgtcacc tttctgattt tcaaatcatc
    20461 aaaaaaatgg gtcgttgtgt gaatgagagg aggaagaaat gaataataca gtaacgaaat
    20521 ggtttcaata cggttttatt ttgctattag gtctagtcat gatttatccg gtcttatgga
    20581 tgatcgcagg ttcttttaaa acggatcaag agattatgag tggttcgctg aatttgattc
    20641 cagagacttt tcaatgggaa aattacgcaa aaggatgggc ggggtttgct ggaatttcat
    20701 tttttacttt ctttaggaat tcgtttgtca tctctgcagt ttcgacgata gggacagttc
    20761 tgagctcgac ttgtattgct tacgcattat cgagaatcga tttcaaagga aaaagattct
    20821 ggttcgtcgt catgttagta acgatgatga ttcctgcaca agtgattttg attccgcagt
    20881 ttattattta caatcggctg aatttggttg ggacgtatgt gccgctgatt ttaccacatt
    20941 tttttggaca agcatttttc atttaccaga tcatgcaatt catggtcagc atccccaaag
    21001 agttagatga atcagcgatt attgacggat gcagcaagta tagtgtcttt accaaaatta
    21061 tttttccttt gctgaaacct tcgatcatca cgacgatcat catccagttt tattggaaat
    21121 gggacgactt catgggacca ttgatttatt taaacaagcc gagatcttac acggtatcga
    21181 ttgccatcaa attatttgca gacgctggat ctagtacgac ttacgcgagt atgtttgcga
    21241 tgtctacgtt gtcgctgcta ccggtttttc tgattttctt attcttcaat aaatatcttg
    21301 tccaggggat cagtacaagc ggtctgaagg gctgaatatg aaaaagccaa tagaagagat
    21361 gtttctttac aagctcaact actatgtctg tcggattttt ttaaccagtc ttatcagtaa
    21421 tgctcttttc gctttagttc taggatgttt gttcacttta ggattagtgc tgtctgctcg
    21481 tgttgtctat ctgatttatt ttttgatgat gtatccttct tattatctat ctgtccatta
    21541 cttggcagaa aaaaaggtat gttggaggaa aggaaaaatc aaacggatgt gtttcatcat
    21601 tacgttgttt cagctgtttg tcattatttt tgcagttgat tggtatttat tagtgggaaa
    21661 gattggagct tggtatctga ttccttttct attgtgtctg ttgctttttg tatcccatag
    21721 tttttttagt acgctaattg atatctgcaa agaagataaa gcagatagtc ttgtttattt
    21781 gatcaaaagg aatatctggc tgttgtcaaa aaacgtcaag gggtttctgt ggtatttatg
    21841 ccttttgtat ggactgttct ggtgtataca gcatgatttt ttactcactg cattactgta
    21901 tccagggctg ccatttgtca cagtattttg cttaaaagaa aaggagaatg actttaaatg
    21961 aagaaaaaat atgtacaagt cggacttggt gggagagcgc gtttttttta tgaggcaatt
    22021 gcctctacgt atcatcaaac ttgtgaacta gtcggttttt gtgatgtatc tcaaacaagg
    22081 atggattatg caaatcggat acttgtgaac aagtatggac agaaaaaagt aaagacatgt
    22141 atgccatcac aatttgagca actactggat gacgtacggc cggctgaagt cattattacg
    22201 tcagtagatc gaacgcatca tgattatatc attcgtgcaa tgaaaaaagg atatgatgtt
    22261 atttgcgaaa aacctctgac tatcgatgag gaaaaagctc aagaaataat aacggtccaa
    22321 aaacagacgg gaagaagcct gcgagtcact tttaattatc gttatgcacc gtatcacacg
    22381 aagataaagg aactgataga aagtgatgtg attggtgatg tgttttctat ccacttcgaa
    22441 tggctgttaa atacgcaaca cggggcagat tatttcagaa gatggcatcg aaataagcac
    22501 aatagcggcg ggcttcttgt tcataaatct acccatcatt ttgatctagt gaatttttgg
    22561 ttgaacagcc gtccccaaaa ggtatttgca ttcggtggat taaactttta tgggatgcat
    22621 aatgcggaga aacgcggagt agaaatattt tacagtcgat gctatggaag cgaactagct
    22681 aaagctgatc catttgctat agacctgtca gccaatgagg aattgcgaga aatgtattta
    22741 gaagcagaaa aagacagtgg atatattcgt gaccgcagtg tattttccga tgatatctct
    22801 attgaagata cactaggcgt aacggtcaaa tatgaaactg gtgcgatttt gacgtattcg
    22861 ctaaacgcgt atatgccgtg ggaaggattc aatgtttctt tcaatggcag caaaggacgg
    22921 atcgaaatga aagtgatcga aaagtcttac atcaatgcag gtggagataa aaaggatgaa
    22981 ggagcagccg ttcttcagtc catccatgtc tatccgatgt tcggtgagcc atatgaggta
    23041 atcgtcgaac aagaagcggg cggacatggc ggaggagatc cgcgtttatt agatgatcta
    23101 tttggtgaag aaaaagaaga agatccgtgg aatcgggcag caactcacgt agatggtatt
    23161 ttgtcgattt tgacaggaat cgcagcaaat cattctattg cttcaggaaa agcagtagtg
    23221 attgacgagc ttgtcagctt aaaagaataa gaatagattt acggaaatta cttcccgtag
    23281 tttataaaat cttgccttat tgccgatagg ctgtcttttg aacactattt attcggaaaa
    23341 aggggctatg acatgctttt tgtcatgcct tctttttatg tgggctgctt ttttagatag
    23401 gagaaaataa aaataactat ttaaacccga acaaaaagga ataatgcatt aaaaatcatt
    23461 agattttaca aaaaaataag taaaccacgt tgtcagtttc agaaagtagt gttagactgt
    23521 tttcaatatt tgaaacaaca ttttttattc aaatatggca aactgccttc acgtgcaaaa
    23581 cagttgcaag aaaatgaatt tacactatta ttagttttac atacctactt atatatcgtc
    23641 ataatatgga tgacagtttc tagccagtac cgtaaatgct ggactataag taaaagattg
    23701 gctatttcac ccttcttttc ttatgataaa gtggagggtg tttttcttta acagaaacat
    23761 agtaggtttt tgcacaaaag cggttatttc ggtattaata aatgataaag gagcaaataa
    23821 agtgaaagaa ttagttgaac gtattcaaaa agacggacgt atattaggcg agggcgtatt
    23881 aaaagtcgac agcttcgtca ctcaccaagt agaccctgta ttgatggaac aaatgggaaa
    23941 acggtttgca gaagtattta aagaacaagg aatcacgaaa gttgtgacca ttgaagcttc
    24001 tgggatcgca ccagctttat ttgctgcaag agaattagat gttccaatga tcttcgctag
    24061 aaaagcaaaa agtttgacga tggatgaaga attattgaca gcttctgttt attcttttac
    24121 gaaacaagtg acaagtacga tttctatttc tcgtaaattt ttatcttcag aagacaaagt
    24181 attgattatc gacgatttct tggcaaacgg acaagcggca aaaggattga tcgaattatg
    24241 ccagcaagct ggagcgcaag tagaaggaat cgggatcgtc atcgaaaagt ctttccaaga
    24301 tggacgtcag ttacttgaag aaatgggatt gagagtcgtt tctcttgcgc gtattgcttc
    24361 cttgacgaat ggacaagttg agtttcttga ggaggacgca taatcgtgga aaaagaaaca
    24421 caaaatggaa aagctgcggt tttagggctg cagcatctgc tagcgatgta tgcaggagca
    24481 gtagcggtgc ctcttttgat cggtacagga ctaggcttta atcaagaaca gatgacttat
    24541 ttgatttcaa ttgatatctt tatgtgtgga ttagcgacat tattgcaatt gacagttaac
    24601 cgatttttcg gaattggctt gcctgtcgtt ttaggatgtg cgatccaagc agtagctcca
    24661 ctgatcttga tcggaaccaa tgaaggagta ggagctatct acggatctat tatcgcttct
    24721 gggatctttg ttgtgttagt ttctggtgtc ttttctaaaa ttaaaaaact attccctcct
    24781 atagtaacag gaacggttat caccgttatt ggtttgactt tgattcctgt agcaatcgag
    24841 aaaatgggtg gaggtaatgc aacagcagct gattttggtg acaaaacaaa tctgctactt
    24901 gcatttgtga cgattctcct aattgtaggc gtgcaaatgc tggcaaaagg gttcattcgt
    24961 tcaatcgcgg tattgattgg tttagtcgga ggaagcatct tagctgcttt catgggaatg
    25021 attgatctga gcgcgattgg agatgcgccg ttgttccatg ttccacaacc cttttatttt
    25081 ggtaaaccaa ctttcgacgt atggtcgatt ttactaatga tcatcatttc catggtcagc
    25141 atggtagaat caacaggtgt atattttgca cttggtgaca ttactgggaa aaatgttgga
    25201 gaagaagaat tgaaaaaggg atatcgtgca gaaggacttg ccgttatctt aggcggtatt
    25261 ttcaatacat tcccttatac tggcttttct caaaatgtcg gattggttca actttcggga
    25321 atcaaaacaa gaaaacccat ctatttctct gcattcttct tgattgtttt aggattattc
    25381 cctaaaatcg gtgcgttagc acaaatcatc cctgagccgg tacttggtgg aggaatgtta
    25441 gtcatgttcg ggatggtggc agtccaagga atccgtatgc tgttggaagt tgatttcagc
    25501 aatgataaaa atctattgat tgttgctgtt tctgttggat ttgggctggg tttcaatgtt
    25561 acgccgacat tattcagtca aatgccgcaa acattgcaaa tgttcactgg aaatggcatc
    25621 gtaatgagta gcattacggc tatcatattg aatttgatct ttaatggatt gaaaaaagat
    25681 gaagaaatat tggaagaagc cttcaagaag taatagagca aaagaaaggt tgtgaaatca
    25741 gcgttttgac ctgattgtta gagaaacaaa cggaaaaata gttcctcgta ttttgccgtt
    25801 tgtttgagct aataccgagg gtcagctgat tgaacaccgt tgaaggaaga ggtcgtgagc
    25861 ctgccgttca tctccgagtc acaaggaaca atttcaaaaa aaagcttctc atgtttgttg
    25921 aatattgttc cttgtgaccg aggagttggc aggagaacac cgtttatcca taaaaaggaa
    25981 tgtgacctaa cttttgtcac attccttttt tttatcttta ataaacacga acaataaaat
    26041 gtttttcttt gttaacattc gaaatttctt tgacatcaaa aaaagtgatt gttaaaatat
    26101 gaatgtagaa aaactgggag gaaaaacaat gacagcaaaa atctctgtga ttatggggag
    26161 tacttcagat tgggaaacaa tgaaagaagc atgtcaaata ttggatgaat tcggtgtgtc
    26221 ttatgaaaaa aaggtcgttt ctgctcatcg aaccccagat ttgatgtttg aatatgcaca
    26281 aaacgctcga aaaaaaggaa tcaaaatcat tattgcagga gcagggggag ccgcgcatct
    26341 gccgggtatg gtcgcatcaa aaacaacttt gccggttatc ggtgtaccgg tccaatcacg
    26401 aacattaaat ggtttggatt cgcttctttc gattgtccaa atgcctggag gagtacctgt
    26461 agctactaca gcaatcggaa aagcgggagc aattaatgct ggactacttg cgatacagat
    26521 gctttccatg tatgatttag aattggaggc taaattggct gaaagacgtt caatgttggc
    26581 taaaactgtt atagaaagta gtgatcaact tggttaaacc tttgttgcct ggttcaacga
    26641 tcgggatcgt cggtggcggt caattaggtc gtatgatggc gattagtgca aaagaaatgg
    26701 gctttaaagt aggtgtgctg gatcctgtga gcgattgtcc agccgcacaa gttgcagatt
    26761 ggcacattga aagtgtctat gatgacacct ttgcgctgaa agagcttgcc agaagaacag
    26821 atgtcattac ctatgaattc gaaaacgtca gtatagaggc gttaaatgcg attttaccca
    26881 tgtcatttat tcctcaaggg acagacttgc ttgcgattac gcaagaccgc ctgttagaaa
    26941 aatctttttt ggaaacgaat aacatcgtga ttgctcctta tgcaacgatc gtcagtccga
    27001 cggatatcca agatgcgatt gacgggatcg gctatccttg tgttttgaaa accactcgag
    27061 gcggctatga tggcaagggg cagtatgttt tgaaaagtcg ggcggatcta gctcctgcca
    27121 tggacctttt aagagaagga acctgtgttt tagaagcatg gatcccgttt gaaaaagaga
    27181 tttcgatcat ggtggcagga aatggtcaag gagatttcac gacttttccg gtcgtggaaa
    27241 acatccacca caacaatatt cttcatgaaa cgattgctcc agccgcgatt gatcaggatg
    27301 tgatcgagga ggctgagcgt attgcccgtg tgattgcaga agcagtgagc ctttcaggtg
    27361 ttttaggggt agaaatgttt ttaacaaaaa caggcgggct atatgtgaat gaattagctc
    27421 ctcgtcctca caattctgga cattattcta ttgaagcttg ttctatgagt cagttcgatg
    27481 cccatattcg aggtatttgt ggttggccat taggagaaaa agtacgtttg ataagcaatg
    27541 cagtaatggt caatgttttg ggagaacaac tgctggaaag ctatcgcttg attgagaata
    27601 agccagactg gcaattccat ttctatggaa aaaaacaagc aaaaaaaggt cgcaagatgg
    27661 gacatatcac tatcttgaca gaagacatcc agcaaacgct ggaagaaatc aaacaaacga
    27721 atatttggga ttagaggaga aaaaacatgt tagaacgtta tacacgccct gaaatgggga
    27781 atatctggac ggaagagaac aaataccgtg cgtggctcga agtcgagatt ttagcagatg
    27841 aagcttgggc agaacttggt gaaatcccgc aagaagatgt gaaaaaaatt cgtgagcatg
    27901 catcatttga tatcgatcgg atcttggaaa tcgaaaaatt aacacgccat gatgtcgttg
    27961 cgtttacacg agcggtttct gaaacattgg gagaagaaag aaaatgggtc cattatggtc
    28021 taacaagtac ggatgtcgta gatactgctt atggctatct catcaagcaa gcaaatgata
    28081 ttttacggga agacttgaaa cgttttacgg aaatcattgg cgaaaaagca aaagaacaca
    28141 aatatacagt gatgatgggt cggacacacg gtgtccatgc tgaaccaact acttttggat
    28201 tgaaacttgc cacatggtat tcagaaatga aacggaacat cgaacgattt gaacatgcgg
    28261 caaaaggagt agaagcagga aaaatctcag gagcagtggg aacgttcgca aatatcccgc
    28321 catttattga agagtatgta tgtaaacaat tggggatccg tccacaagaa atttctactc
    28381 aagttctccc tcgggatcta catgctgaat atttctcagc gatggcgctt attgctacaa
    28441 gtattgaacg ttttgcaaca gagatacgcg gattgcaaaa atccgaaaca cgtgaagtag
    28501 aagaattttt tgcgaaaggt caaaaaggtt cttctgctat gccgcacaaa aggaatccga
    28561 tcggttcaga aaatatggct ggtctagcca gagtggtgcg cggacacatg gtcactgcct
    28621 atgaaaatgt cacattatgg catgaaagag atatttctca ttcttcagca gaacgaatca
    28681 tcttgcctga taccacgatt ttattgaatt acatgttaaa tcgttttggc aatatcgtta
    28741 aaaatctaac ggtctttcct gaaaacatga agcggaatat gtctgcaaca tttggtttga
    28801 tttatagcca gcgcgttatg ctgaaattga tcgataaggg aatgacaaga gaagaagctt
    28861 atgatcttgt tcagccaaaa actgcgtacg cttgggacaa ccaagtgcag ttccgcccgc
    28921 tgttagaaga agatccaacc atcacatcgg tattatctaa agaagaaata gacgatgcct
    28981 ttgattatca tcatcatttg aaaaacgttg acgtgatatt caaacgagta ggtttggaat
    29041 aaagaagagt gagttgcaaa attcaattaa aatagaatag caaaaataac caaaaaagaa
    29101 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
    29161 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
    29221 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
    29281 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
    29341 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
    29401 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
    29461 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
    29521 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
    29581 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
    29641 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
    29701 gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt
    29761 gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat
    29821 ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac ttttgtatga
    29881 aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga tagaatattt
    29941 agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa aaggagaatt
    30001 gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa taaaaaatca
    30061 ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa ttatccctct
    30121 ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag gtgttcccga
    30181 aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc agttggatga
    30241 tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg aagaaatcag
    30301 tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat ttgctaaggt
    30361 cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac aaattttgct
    30421 tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga atgaacatat
    30481 ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc aagaagtata
    30541 taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta
    30601 tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa aaaagcagtc
    30661 catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt tgagatacaa
    30721 cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga caagctgctt
    30781 gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga agtataacat
    30841 gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt tatgggcagt
    30901 tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct ctttagaagg
    30961 atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga gatgcggtgc
    31021 gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag aagggaaacc
    31081 agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt tgccaggtgt
    31141 actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga aagtcaaccc
    31201 caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc cagttgctca
    31261 tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa aaaataatca
    31321 gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg cagggattac
    31381 gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag tagaagactt
    31441 gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact tcggaaaggt
    31501 gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat
    31561 ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag atatcttagg
    31621 aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg aacattgttc
    31681 ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc atgtgttgca
    31741 aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag ttgtcttcaa
    31801 agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg cgacaggagt
    31861 tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt tgctagattc
    31921 attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag aagtagttgc
    31981 aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg aaattgcatt
    32041 tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt tgattgaaca
    32101 caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc
    32161 aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat ttaatcaaga
    32221 agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa agctgctgct
    32281 tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta tacaggatat
    32341 gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg gctcaggact
    32401 tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt atgaaatgat
    32461 gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat
    32521 ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag taacagacga
    32581 cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag tcgatgcatt
    32641 agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt
    32701 tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat tgcttgatct
    32761 gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg attcacaagt
    32821 acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg ttagagggac
    32881 gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt tggatccaaa
    32941 aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta gcggtggtaa
    33001 gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag aagttttttg
    33061 ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag gaactcctgt
    33121 tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct atccaacacc
    33181 aatgattggt atggtcggat tgattgagga tcataaacat atcactactc aggaattcaa
    33241 aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg atggcagtga
    33301 attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg atctatccat
    33361 tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga ttgaaagtgc
    33421 tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt tcaaacatca
    33481 gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc
    33541 aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa tgatgggaga
    33601 tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt cagcaactga
    33661 cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg
    33721 tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca
    33781 gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg acaagaaggt
    33841 gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt ggggctgctt
    33901 tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc tgcaatcgga
    33961 catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc ttttctattc
    34021 aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa tgctcgttcc
    34081 cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga tacggagata
    34141 ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt aaaaagtgct
    34201 ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc aatgattgcc
    34261 gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa cggagcatac
    34321 gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat aagagatgtc
    34381 cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg ctttacgaag
    34441 gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc agattcaaac
    34501 atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt agcgaaagaa
    34561 tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc agccgcaagc
    34621 ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa tcaatacatt
    34681 gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg aatgaaatta
    34741 tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt
    34801 cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc aaaagaagtc
    34861 catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat tgatattcaa
    34921 acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga atgtatcggt
    34981 gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa
    35041 gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc gacgccgtta
    35101 tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag
    35161 cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga
    35221 tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa aaacggaacg
    35281 tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat cggtctttca
    35341 attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat tagcagtggc
    35401 aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga
    35461 tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa cggggaaaaa
    35521 tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa
    35581 tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag gtaatgatta
    35641 tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga caggagaaac
    35701 gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt caaatgggta
    35761 ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat caaaactgcc
    35821 agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct atgtttcttc
    35881 cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca ctggtggagg
    35941 gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa tcaaacttgg
    36001 ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa ttcctgagat
    36061 ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcg aagaatcgaa
    36121 agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg aaatcgggaa
    36181 agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat gagaatcgct
    36241 gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct atcgaaaaaa
    36301 ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa
    36361 cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt tgattcaaaa
    36421 aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta
    36481 ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga taaaagaatc
    36541 atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat acgtgatgcc
    36601 tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca aggtgtagat
    36661 actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac gttcgattct
    36721 ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat tagtgaaatt
    36781 attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca gataaaactg
    36841 ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt tcaacaggag
    36901 gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag
    36961 ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc catggtggtc
    37021 ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa aatatccaac
    37081 caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg aaaccagatg
    37141 tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg ctccgtagtg
    37201 cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat gaagaagtac
    37261 tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa ctagcggcaa
    37321 aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta acggattggg
    37381 tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa acgcttcgtt
    37441 acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg gaaaaatatt
    37501 cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat atccgagatg
    37561 cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc gccttgaaac
    37621 acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg gaagcagcgt
    37681 atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga gaagtagatc
    37741 tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc ccttcttata
    37801 cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg acagtagact
    37861 ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt ggacttcttg
    37921 tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta
    37981 agccgactcc agcagaagca aaagcattag tgtttgcttg gagagctgtc aaacatgtga
    38041 aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga gccgggcaga
    38101 tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg
    38161 cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat gcagccaaac
    38221 atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat tctattgata
    38281 tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt agacattaga
    38341 gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa catgcaataa
    38401 gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg
    38461 ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa
    38521 tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa gttcctttgt
    38581 taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt ccgacaaaag
    38641 cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat acttatcaaa
    38701 tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg tatatcaaag
    38761 aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg
    38821 tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac cagcatcgat
    38881 ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa gaattctccc
    38941 ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa gatcataagc
    39001 gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat tcaccagtac
    39061 cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa ccagcggctt
    39121 tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt ttgattgcta
    39181 cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct gaaacgcaag
    39241 tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta ttagagaatc
    39301 gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg gttgcagctg
    39361 ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg gaagaagagc
    39421 tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga gcaggaggac
    39481 gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa aaaatttaca
    39541 ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt ggagaaaaag
    39601 ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa aaaatacaac
    39661 aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt ggtacacttt
    39721 tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg tacttctgat
    39781 cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga agatccagta
    39841 aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc agatatttat
    39901 ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc aatcaaacga
    39961 gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt tgatttttct
    40021 aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg
    40081 gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt agctgaagcg
    40141 tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca
    40201 atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa
    40261 tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat gacagatgaa
    40321 gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga tatgacgatc
    40381 caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa
    40441 ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac gacaccaact
    40501 ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat cgccggtacg
    40561 atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat gtctcgggaa
    40621 gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt gtcataagca
    40681 ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt cagcttcgag
    40741 atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt tcgtttatta
    40801 gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa tcccttgttt
    40861 tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa aagcaatatc
    40921 tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc tttgttctgt
    40981 cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga aatgagaggg
    41041 acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg taaaattttc
    41101 tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg aaggctgtga
    41161 gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc
    41221 ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt gcgggatttt
    41281 cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac gatataatcg
    41341 tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag atacttcgtt
    41401 tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat caactggaag
    41461 tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg aactggcggt
    41521 ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac
    41581 aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat ggaccaacgt
    41641 ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa ctattaaaaa
    41701 tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc taaataaaaa
    41761 cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc
    41821 taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag caagtttcaa
    41881 tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat tttttttcaa
    41941 ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac
    42001 tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca gataatcatc
    42061 tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac taataaatat
    42121 gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa
    42181 aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg aagacagtgc
    42241 catctcacca tctccagctt cgccgacata agtgattgga taataagaac caaggacagt
    42301 tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa aaactttata
    42361 catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg acagttgttc
    42421 cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa taaagaagga
    42481 gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg atccctttgg
    42541 ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg ttgtctataa
    42601 ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat cctttgtgac
    42661 ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag atcattttta
    42721 aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc atatggccgt
    42781 atcttatttt taagatgact agataatcat tgatatggct aatttctttt tcaagaggaa
    42841 tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag
    42901 tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc aaactgttat
    42961 aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt gctaaatggt
    43021 attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt ttattcatgc
    43081 tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag ccaaggttcc
    43141 ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg acctttctct
    43201 ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact ccaagttcca
    43261 ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga
    43321 tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca gaatctacct
    43381 gattgatgat ctcttcaatg gtatccataa aatcttttgc caagatatta ttgatcagct
    43441 cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac gtactaattt
    43501 ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt tcttctagga
    43561 tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc cacatacttt
    43621 ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg
    43681 tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc aaagggataa
    43741 cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt
    43801 ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat attctgctgg
    43861 gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt ttgaatattt
    43921 ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt acactcgact
    43981 acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt
    44041 acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc taaagcaccg
    44101 tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc atttgtttct
    44161 aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac aattcggtga
    44221 acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt tggtttacga
    44281 ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca
    44341 gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat
    44401 aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg
    44461 ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc tcgctttgct
    44521 tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat catatccact
    44581 ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat gtagccattt
    44641 cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc agtatgcttt
    44701 ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct
    44761 tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa
    44821 aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta tcttatcaga
    44881 taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa
    44941 atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg
    45001 ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga taaaaaatga
    45061 aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc attctattaa
    45121 gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga atcgctatgc
    45181 cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg gaattagaaa
    45241 aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat caagtagcac
    45301 aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct atcgatggtt
    45361 cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg
    45421 atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat aattttggag
    45481 taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa ggaaaaggac
    45541 cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta atcaattaca
    45601 acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc gtgccatctg
    45661 gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg gcgcaagcaa
    45721 ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct gttttgtcgc
    45781 catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg
    45841 aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc gttaaatcga
    45901 tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta gcaaaaaata
    45961 cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat gatactgaaa
    46021 cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta tctgtcgata
    46081 aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc gatctgaaaa
    46141 attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga taagaattat
    46201 ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa tccgagcgtt
    46261 agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg
    46321 agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg gctcctatga
    46381 cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga
    46441 aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt caataaaaga
    46501 gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg aactgacaga
    46561 aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga atcttctagt
    46621 atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat tatcaaaatc
    46681 cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag aaagcgcgaa
    46741 tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca ttatttcaca
    46801 taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg atggacagac
    46861 gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg gaatggtggg
    46921 cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag tttattttaa
    46981 agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa acgaccacat
    47041 caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg gagcgggcag
    47101 aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat ccggacgtat
    47161 attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg caaacggact
    47221 cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg atattcggga
    47281 aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag ataaagaaaa
    47341 agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa actccatcca
    47401 tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct
    47461 gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag atgttggggc
    47521 aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag ctgtctgtct
    47581 tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata cgatgaacga
    47641 aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct tgatgaaatt
    47701 aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg gataaaaaag
    47761 agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac agcatggtga
    47821 tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt ttctggcgtc
    47881 cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta gctgcaggga
    47941 tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg
    48001 ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg atgacagtta
    48061 tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg gtagcttatg
    48121 cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt ggactgacac
    48181 aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa aaaatgtcta
    48241 cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca ttgattttag
    48301 gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa
    48361 atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta tttacagcag
    48421 tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg attattttga
    48481 tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca gtagcaggtc
    48541 gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg atcaaaacaa
    48601 agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta gctggattag
    48661 ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg gaactggatg
    48721 caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga acaatcatgg
    48781 gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg attttaggcg
    48841 tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc gttgtgctag
    48901 acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca gaaaaaagac
    48961 tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca ttgtttcgtt
    49021 tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc tttttgctct
    49081 tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc ttgattaaat
    49141 taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat gaaagtattt
    49201 gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg ttctgaatgt
    49261 gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc tgccaacatg
    49321 cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta tttctatatc
    49381 atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga
    49441 ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat agagaagttg
    49501 gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg acatgctaac
    49561 tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc
    49621 ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc agatgcaaca
    49681 aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg ctttgggact
    49741 ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc
    49801 gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt tggtgcgaca
    49861 atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga aacaaaagtc
    49921 gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca aaaaggcgaa
    49981 aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt gaaagataca
    50041 ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg aagagatctg
    50101 gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca
    50161 atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata
    50221 agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt attatcgcag
    50281 atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt tttgtcacag
    50341 tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta aaatcaaaag
    50401 aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa aaacaaggac
    50461 cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga aggatcaatc
    50521 ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg aatcgttcca
    50581 ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc aaacgtggca
    50641 gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga atcgattggc
    50701 ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa ataagagaag
    50761 caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg ccagatgagc
    50821 ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag
    50881 aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat ttggaaagta
    50941 aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa
    51001 tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta gagtcacctt
    51061 ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt gggataccga
    51121 tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa aataaattga
    51181 aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg agtcaaaaac
    51241 gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt gaagacgatg
    51301 tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga acgattgatc
    51361 cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg
    51421 gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa gaaatgatgg
    51481 agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat gaagcaattg
    51541 atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt
    51601 ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac tatgcctgga
    51661 ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta gatatgggtc
    51721 ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga atcaattaca
    51781 gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt
    51841 ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct tggatttaaa
    51901 tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact aagaaaaaac
    51961 cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa caaaactgta
    52021 taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa aatagaaaaa
    52081 atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat gctgttaata
    52141 tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt gtatttctca
    52201 taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt tattcactgt
    52261 ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg acaaatggca
    52321 aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc agatttccca
    52381 gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt acctgattta
    52441 tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt attagaccaa
    52501 cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa
    52561 gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct tcttccaggt
    52621 tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc ttttttatgg
    52681 tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct accaccacaa
    52741 attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt gaagaaacga
    52801 aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca aggtgatttt
    52861 tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta tatccaaaac
    52921 aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa
    52981 aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc gcttgcagta
    53041 tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc agtcctaatc
    53101 gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc aacccgctac
    53161 cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg cttccgtaaa
    53221 tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact
    53281 aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca aaaggtttca
    53341 gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt
    53401 tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa gcaatcacag
    53461 atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg
    53521 ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc ggagaagacc
    53581 gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt
    53641 tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc gaagcaatgg
    53701 acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact agaaatctac
    53761 ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg cattcggata
    53821 atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct tccaaccctt
    53881 atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt gttttcctag
    53941 aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct tacgaagttc
    54001 ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc acatacggag
    54061 caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat atatctgttg
    54121 aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt caatccgtgg
    54181 aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc gttgccgcgc
    54241 aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca atcggacgag
    54301 tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg
    54361 caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa
    54421 acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc gcagaaggtg
    54481 aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat acattactag
    54541 aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga acggttaaaa
    54601 atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta gaaatcgatg
    54661 caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc caaactccag
    54721 ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca agcgaaggag
    54781 tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa atcgtcaaat
    54841 ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa gttcaaaatg
    54901 acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac gtgattgttc
    54961 cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct cctggacaca
    55021 atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat
    55081 caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg gctatgccat
    55141 ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact gcaactggaa
    55201 aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg
    55261 caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct cctgcagcag
    55321 aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac ttggaagaac
    55381 gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac agtaagcata
    55441 cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg gataatcgga
    55501 aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg ccatatgtcg
    55561 tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct atcgacgatg
    55621 caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact gatacggatc
    55681 acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt gctattgcag
    55741 acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca gaagatatgc
    55801 gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg tttacaccag
    55861 tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa caaccaatcg
    55921 taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc
    55981 accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa cgtcttttag
    56041 ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg agattttgct
    56101 gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc tgccatccgc
    56161 gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg cggtgtttgt
    56221 ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca ttatcaagag
    56281 tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa
    56341 acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt tggtttccta
    56401 ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca
    56461 ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc aatcatcgca
    56521 acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt gttagattct
    56581 actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg tggcggtgtg
    56641 attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac gattcttgaa
    56701 ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt agaagatgat
    56761 ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac
    56821 ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt tactgctgat
    56881 tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt agaacaagcg
    56941 ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg tacgaacgta
    57001 cctaacatct atgccatcgg ggatatcgtt ccaggggcgg cattagcaca caaagccagt
    57061 tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt agattataaa
    57121 gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat gacgatcaaa
    57181 gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc agggaacggt
    57241 cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac gatcgaagat
    57301 aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta
    57361 gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat tcatcctcat
    57421 ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt accaatccat
    57481 atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta
    57541 gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg gtttattacc
    57601 acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg caagaatatc
    57661 agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg aagatgtggg
    57721 aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg aagacttatc
    57781 agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga cgggaatttg
    57841 aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca
    57901 aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata tcaagtcctg
    57961 gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa
    58021 atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt ttctaatcga
    58081 aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag ggtgtaatca
    58141 attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca ccttgaattt
    58201 tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg gcgttggaaa
    58261 gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa aaggaatagg
    58321 cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat cggaaggctt
    58381 acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag aaattgcgga
    58441 aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg caatcttgaa
    58501 aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag cgggaaatgt
    58561 cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt tgacatttga
    58621 aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg cagacctttt
    58681 gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa
    58741 agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa aagtctgcta
    58801 taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa ggagttagtt
    58861 acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga
    58921 aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc acatacgcaa
    58981 ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta
    59041 gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga tactccagga
    59101 cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg
    59161 gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa aaaagcactg
    59221 gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc tgctcgtcct
    59281 gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga tgatgatcag
    59341 ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt gtcagatgat
    59401 ccagcagatc aagaaccaac catggcacct atttttgata caattattga aaaaatccct
    59461 gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact tgactacaac
    59521 gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa agtaggagac
    59581 caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac gaaattattt
    59641 ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga cttgattgct
    59701 gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat
    59761 gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt agttaataat
    59821 tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga agaacgtttg
    59881 atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc tccagatgca
    59941 tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa catgcgccgt
    60001 gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat cgacggtgta
    60061 aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat gggtagtgtt
    60121 atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac aggaaacgga
    60181 caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc aactgaattc
    60241 ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata tctgccaatg
    60301 cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa
    60361 gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt
    60421 acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga tttgactgtc
    60481 aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga ccaaacatct
    60541 gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt gaatgatgat
    60601 gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt agagaaaaat
    60661 gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag agagaaacca
    60721 gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt ggagaagtca
    60781 tctttgtcaa tggacataaa ttcagaaaat acgccgaaag gcgtttaaaa gacttttaaa
    60841 aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga aaggagatca
    60901 acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg
    60961 acttattaaa acaatacttt ggctatgatg agtttagacc tggtcaaaaa gaaattatcc
    61021 agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt gggaaatcaa
    61081 tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg tcccctttaa
    61141 tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc gccactttta
    61201 tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc gatagacagc
    61261 agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt atccaagcaa
    61321 tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc tctcagtggg
    61381 ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa ctgccaaatc
    61441 gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct gacataaaaa
    61501 gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga gaaaatctac
    61561 gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat ttaaagatca
    61621 acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac cgtctctatc
    61681 atttgcttaa aaaattcgat ttttccgtgg gtagatatca tggtggtcta aatgagaatg
    61741 agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt gtagcgacaa
    61801 atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat tatcaaattc
    61861 ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat ggtctttcca
    61921 gtgaagccat cctgcttttc tctcctcaag atatccaagt ccaaaagttt tttatccagc
    61981 agtcccaaag agaagaaggg caaaagcaaa aagaatatga aaaattaaaa gcgatgacag
    62041 aatacgtgta tattgagtcc tgtcttcagc aatacatcct aaactatttt ggcgagacaa
    62101 gcagtccgtg caaccgttgt ggtaactgtt tagatgacag agaaatagta gaagtaacga
    62161 cagaagcaca gatggttctt tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa
    62221 tgctgatgaa agtcttagca ggatcgaaag aacaaaagct gcaagcttta ggttttggac
    62281 atttatctac ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg
    62341 aatatttgat atcgaatggc tatcttctga ccatcaatgg tgagtatcct gttttgaaag
    62401 taacagaaag agggatacaa gtattgaaag gacaagaatc tgtttatcga aaggaaccca
    62461 agaaagtcca gcagctatcg gatgaagaaa ccgatacact atttgaagtg ctgcgggaac
    62521 tgcgaacgga tctagcatca gaagcaggcg ttcccccgta tgtcgtattt tcagattcca
    62581 cattgaaaga aatgagccga atacgtccat caagcagatt ggagatgctt cagataaaag
    62641 gagttggcca aagcaagctt gataaatatg gcgaagcctt tttatctcgg ataaaaaatg
    62701 cggactctaa cgttctggat aagtagaaag aacgaatacg taaaaatgaa taaaaacaac
    62761 tggttaaatc tgtttttttt gctccttatg aaaatcaaat atgttataat taaggaatta
    62821 gaatgggagg taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa
    62881 tgatgaagca gaacggatca aacgattaat ccgaaatcaa aacaacagtc tctgtatctc
    62941 acaatgcaaa gccttcgaag aagtggtaga tacacagatg tacggctttt cccggcaagt
    63001 cggttttgct gtccgtatag gagtaatcgg aaagaacgag ggccagctga tgttaagtga
    63061 gctagaacga gaattgaatc agttatatag tgaggtttac caagaaattt atgataaaaa
    63121 agaagttggc aaggaggaat agacttgcct aagaaagtga aaaaaaggca tctattagac
    63181 tatagcatct tgattcccta tttaatttta tgtgtattgg gattgatcat ggtatatagt
    63241 tctacgtcgt atctattatt agaaaacgga caaaatccgt ccgcatcagt cattaaccag
    63301 agtatttttt gggtgcttag cttgattgct atcgcactac tttataaaat gaaaacagat
    63361 gttttgaaaa accagcgttt gatcatggca gccatcgcag ttttgacgat tttgctgctg
    63421 attgttgtgt tttttggtaa ggagatcaat ggggcgaaag gttggctgca aattgcagga
    63481 ttctcgatcc aacctgccga atacctaaaa atcatttcta tctggtacct gtctctgact
    63541 ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa acgtccactt
    63601 gcaatggtta tcggacttac tgcattagtc gctattcttc ccgattttgg taatgcggca
    63661 gttatctttt tgattatttt ggttctgctt ttagccagcg gagtcaatta tgtgtatact
    63721 ttgattgtag gagtaggagg cttttgtcta agtactttta cgatctggct gatcaatatc
    63781 acaaacggaa agatcttacc cggacgttta cagtatatct ataatcgatt tgctatttac
    63841 caaaatcctt tctcagacga attaaataaa gggcatcaac tagtcaacgg ctactatgcc
    63901 atgttcaacg gaggactatt tggcagggga ttaggtaaca gtatccagaa aaaaggattt
    63961 ttacaagaag cacaaaccga ttttatttat gcaatcgtag tggaagaact gggagtgatt
    64021 atggggattt tgatattagc gttgctattc tttatgattg tgcgcattgt ccttgtaggt
    64081 atccgttcaa aagatccgtt caattcgctt ttgtgcattg ggatcggcgc aatgtttttg
    64141 atccaagtat ttgtcaatct tggaggaatt actggcgtta ttcctttgac agggatcacg
    64201 ttcccatttt taagtcaagg aggctccagt ttgctgatgc tttcgatttg tgtcggcttt
    64261 gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag gtttataata aaaaaagatg
    64321 taggagtggt tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt
    64381 atttcgtgct tgtacagaat taggaattaa aactgttgga atctatgcag aagaagatga
    64441 atattccgta caccgtttca aagcggatga agcatattta gtaggacaag gaaaaaaacc
    64501 gattgatgcg tatctagata ttgaagggat catttctatc gcgaaagaat gcggagctga
    64561 cgctattcat ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga
    64621 agaagaaggc atcatttttg ttggacccaa actgcatcat ttagatattt ttggagataa
    64681 aatcaaagca aaagctgcag ctatcgaagc ggggattgct tcgattccag gcacagatgg
    64741 accaatcgct tctatagacg atgccttgga gtttgcgaaa caatacggtt atccgattat
    64801 gatcaaggca gctctaggcg gcggcggacg cggcatgcgt gtagctcacg atgaaaagag
    64861 tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga
    64921 agtgtatgtt gaaaaataca tcgctaatcc taagcatatc gaagtacaga ttctcgggga
    64981 tacgcatgga aacgtcatcc atctttttga aagagattgt tctgttcagc gccgtcacca
    65041 aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg
    65101 ccaggcagct gtccaattga tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt
    65161 tcttgttgaa ggagatgatt tttactttat cgaagtcaac cctcgcgtcc aagtggaaca
    65221 tacgatcaca gaaatgatta cagatattga tatcgtaaca acgcaactgt tgattgctca
    65281 aggtttggat ctgcataaag aaatcggatt gcctcagcaa gaaggaatta aattaaatgg
    65341 ttcagccatt caatgccgaa tcactacaga agacccgttg aataactttt taccagacac
    65401 agggaaaatc gatacgtatc gttcacctgg tggttttggc gtacgtctgg atgtcggaaa
    65461 tgcttacgcg ggctatgtag tgacaccgta ttttgattca ttgctagtaa aagtatgtac
    65521 gcatggtgct acctttgaaa cagccattca gaaaatggaa cgttgcttga aagaatttag
    65581 aatccgtgga gtgaaaacaa acattccatt tatgctcaat gtcatcacac atcctgaatt
    65641 ccaatcagga aatgcaaaaa caaccttcat tgatagtaca gcaacattat ttgaatttcc
    65701 tcgactacga gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg
    65761 ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga
    65821 tctaatcaca agaactgatt acgtaacggc taagaatgta ttagatgcga agggtgccga
    65881 tgctttggtc gaatggatca aaggacagga aaatttgttg ttgacggata cgacatttag
    65941 agacgcccat caaagtctgc tagcaactcg tgttcggaca aaggatttca agcagattgc
    66001 acgtttgact ggagaaggtc ttcctgaact cttttcaagt gagatgtggg ggggagcaac
    66061 atttgatgtg gcttaccgct ttttgaatga agatccttgg caacggttga gaaaaattcg
    66121 ttctttaatg cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta
    66181 ttctaattat cctgacaatg tattggtgga atttgtcaaa gaagcagcag cgcaaggaat
    66241 cgatgtattt cgaatctttg atagtttgaa ctggactcca caaatggaaa aaagtatcca
    66301 agctgtacgg gatactggta aaatcgcaga ggcagctatt tgctatactg gcgatatcaa
    66361 cgatccaagt cgggcaaaat acaatgttca gtattataaa gacatggcaa aggaactaga
    66421 acaactgggt gcgcatatca ttgcaatcaa agatatggct gggctgctta aaccgcaggc
    66481 agcttatcgt ttgattagtg aattgaaaga aacgacagat ctgccaatcc atctgcatac
    66541 tcatgatacc agcgggaatg gaatcatcac ttattctgct gcttcaaaag caggagtaga
    66601 catcgttgac gtggcaatga gtgcaatgag tgggaatact agtcagccta gcatgagcag
    66661 cttgtattat gcactggtca atggtccacg gctgccagaa atcacgattg aaaatgcaca
    66721 gaaattaaac cattattggg aagatgtgcg catgtattac aaaccgtttg aaaacggctt
    66781 gaatgctcct gaaacagaag tatatatgca tgaaatgcca ggaggacagt attcgaatct
    66841 acagcaacag gcaaaagctg tcggattagg tcatcgctgg gatgaaatca aacaaatgta
    66901 tcatactgtc aacttgatgt ttggcgatat cgttaaagta actccatcat ctaaagtagt
    66961 tggagatatg gcactattta tggtccaaaa tgatcttaca gaagaagata tttatgaaaa
    67021 aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag aactagggca
    67081 acccgttggc ggatttccta aaaaattgca aaagattatt cttaaaggca gacctgcatt
    67141 gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt
    67201 ggcagaaaaa attggctatg aaccaaaaca agacgaagta ttaagctatt tgatgtatcc
    67261 gcaagtcttt ttggactatc aaaaagcata taatcaattt ggcgatgtta cattactgga
    67321 cacaccaaca tttttccaag gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg
    67381 cggaaaaacg cttatcattc gtttagatga aatcggagaa cccgatatcg aagggaatcg
    67441 cgtactgttc ttcaatttga atggacaaag acgtgaaatc actatcaaag ataattcgat
    67501 catcagtacg gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac aaattggagc
    67561 aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg
    67621 tgatacgttg atgattactg aagcgatgaa aatggaaacg gccatcgaag cacgttttga
    67681 cggagaagtc gcacatgtat atgtgtcttc aggagacact atttcgtctg gtgacctttt
    67741 gattgaagtg actgagaaat aataagcgaa aggtggggaa acatgaaaag actgggtttg
    67801 ttttggctga ttctgctatt agttattgtg gcattctaca tggaaccagt cttgtttccg
    67861 ccaaaacttg aagtgaagga aaactctgat caacaaatat caaaccaaat gacaacgtgg
    67921 aaataccagg agcttaaagc aactggtttt tctacttata tcggaaaacc gatcgatcag
    67981 ctggaaaagg attttggcca agcctatgat cgattaacta gcggatttgg ttttgaaaca
    68041 agatattatg aaaataaagc tagtcatctc acatttgaag cgaacatcca agacagcaga
    68101 gtcacaactg ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg
    68161 atgacgatgc aagatcttac gaaaatcact actatctata ctaatttcac ttttgagtac
    68221 catgatacag aagttggaat cgaactaatg gaagaggata tgaattatcg tccgctaatt
    68281 gcttttgata acgacacttt tgctatttta ttttttgatc aaacaacaaa cggcctattt
    68341 tcagttgtgt atttagataa agatagtctt ttgaaactat tgccatatca agtgtttggt
    68401 gaaggccttc cacattatca agcagataaa tcggctgatt gggaatcaat caatcgcgtt
    68461 aaagaacgaa aaagtacgac tttattaaat atgcttagaa gagaagacga acttcccata
    68521 tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc tactacatga ttttttgaca
    68581 aggcctgagg aaatattgtc agaggaacgt atggctgatt ggcaaaaatc gctaggcagt
    68641 gctcaaacga cagtgaaatt ctcactgacg aatgatgaat tggagaagtt aatcagtaaa
    68701 caaaaattag aaaatactag tgggctattt attcatccgg cgattgacgc tactttttcg
    68761 tttttatatt ggtacagcga tccttacaac catgaccgtt tcatggaccc gggaacggat
    68821 tctgtaggca ttgctttttc taaagaaaat atgttagtct tattgcaaga agaaaacgga
    68881 gaaagtagtg atagcagtga tcgttaacga agagctgttt caactggagg atcaatgcct
    68941 ccaagtcgct gaaatgataa aaaagagtaa aacgatggct tcttatcttg aacataaaca
    69001 gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga
    69061 agcatttgaa aatgtagcgg catatggtga gtatgcacct gattatcgtg aaaaaaaacg
    69121 agctgttcga aaagcaaaaa gacagcttga tatggatgag catgttgcag ctttccgata
    69181 tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc caagtacttg ccgaagaagt
    69241 ttctaatgaa atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa aacactttgg
    69301 atgcggaggt aattgtcatg caattagaca atagtgaatt tgaaataaca aaacgacgcg
    69361 ggctgatcgt atgggtctac agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt
    69421 tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa gagacattgg
    69481 aagaaacaga agaacgaatc cgtaaattac attttgttcg aaacgtagaa cgttcttacc
    69541 gaccggatat cgaaatgaat tttgccgaaa aaataggaaa caaaacagta ttaaaagacg
    69601 aaggtttcga aattgaagag ctaaatacag aaatccgatt agctgaacat ctttaaaaat
    69661 ctcttatgag cagtttttaa ataaaaaata acttggataa cgatcgattt ttgatcattg
    69721 tccaagtatt tttttggtat actagtacca gtaccatttc taaggagtga gtagatgcgg
    69781 ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga
    69841 ccaactacag ataaagtaaa agaatccata ttcaatatga ttggaccata ttttgatgga
    69901 gagacagtat tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc agtatctaga
    69961 ggctgcagtc atgcggtatg cgtagacaaa aattaccaag cgctaaaaat catcaaagaa
    70021 aacatcgaaa ttacgaaaga accagagaaa tttaccacaa taaaacttga tgcggacaaa
    70081 gcaatcgaac ggctagcagc ggagaaacaa caattcgact acttgtttct tgatccgcca
    70141 tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg aagaactggg acttttttct
    70201 aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg aattgccaga aactgtggct
    70261 ggattccatc aaataagaaa acagatttat ggcataacgg ccgtaactat ctatagaaaa
    70321 gagggaaatc aatgaaacgt gcattattcc caggaagttt tgatcctttt acaaaaggtc
    70381 acttagatac ggtggaacgt gcggctaaat tattcgatga ggtagtaatt ggtgttttta
    70441 tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag aatgacattg atcacaaaag
    70501 ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa
    70561 cagcaaaaga gattggtgca gatgcgttga tcagaggcat acgaagtatc aaagactttg
    70621 aatatgaacg agagattgct cagatgaatc atcatctata tgatgaattg gaaactgtct
    70681 tcttattagc aaaaccagaa tatagtcacg tgagttccag tattttaaaa gaagtactgc
    70741 actttggcgg agatgtgagc agctacttac cgccagtgat caacgaagct ttagctggaa
    70801 agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag tactgattat catatttgga
    70861 atgttgatca ttggatttgt agtagttccc ttgccgtatt atgtagaatc accgggagcc
    70921 acgatcgatc tgaaagaact aattacggtc aatgataagg aagataaagg ttctgggtct
    70981 ttctctctta cctctgttgg tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa
    71041 atgacccctt ttgaagaatt gatcagtgaa gaagaattga ctggtggggc gacaaatgaa
    71101 gaatataatc aaatgcaaca gtattatatg gattcttccc aaaatgcagc aatcgaacaa
    71161 gcgttaaaac ttgcagacaa gccttataaa atggaattca aaggcgtata tgtcatgagt
    71221 gtagagaaaa catcgaattt ctttggcaag ctttcagttg gagatacagt aacaaaagta
    71281 gacgggaaaa gctttcaaag tacagaagca tttatggatt atgtaaaaag ccaaaaagtt
    71341 ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc ttctggtgat
    71401 ctgatcgagt taccaacaga taaaaaggca ggaataggta tcacgcttac tgaccatacc
    71461 gagatcgaat cagatacaga tgttcgtatt aattctggct ctatcggtgg gccatcagcg
    71521 ggattgatgt tcactttaga aatctatgaa caagtcactg gtaaaaactt gcgacatggt
    71581 aagcaaattg caggaactgg aacgatcaac agtgaaggag aagttggccg aatcggagga
    71641 atcgataaaa aagtggcaag tgcggataaa gctggcgtag agattttctt tgcaccagat
    71701 gatacgattt cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa
    71761 gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta
    71821 caagaagcgc ttgactatct agagaaaatg aaatgaaact aaataattaa agaggggttg
    71881 tgacacgagt tctgtcacaa cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa
    71941 acaaaagaaa aaattgacgt aattagatgt tgaagggacg attttgccag agctcactgt
    72001 ttcttcaatt ttaaatgaaa ggagtaatga atgattttta ctggtcattc tttgaaaaaa
    72061 gaacaatgat agaaaaaatc agcaagaaaa tactagcttc acccaaattg atcatttgga
    72121 tgtctatgat agttcttatc ttttgtatcg caagtggttt tttattatca aatttgctat
    72181 tttatagaga aacagaagag actgctgcaa aagaaatact ttcttcgttt agttccagtg
    72241 gagaagatcc atcagctgaa ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt
    72301 acaccgatat caaaggaagt gtgaaggagc ctggtatcta ttccttttct tcagaagaac
    72361 gggtctatga tgtactaaag cgtgcaggtg gattattaga agaagcggat agcgatcgaa
    72421 tcaacttttc tgcgaagata gaagatcagc aagtcctata tatccctgct gtaggtgaag
    72481 agctgccaga acatcttgat cagttggcat cctcggaagg aaaacagcct acagcagatt
    72541 ccgagcctcc caaaataaat atcaatacag ccactccatc agaacttcag cagattcctg
    72601 ggatcggatc agtcaaagcg caagaaatca ttcgatttag agaagaaaac ggatccttcc
    72661 agaaagtgga agacctgcag gagatttctg gtatcggtga aaagacagtt gaaaaactaa
    72721 aaaacttcgt tacaattaaa tagaatgatt caaaatcgac tggcacgagc aataaatgga
    72781 cgctaatgga tactaagaat aatccaattc taagagcttg gcaggacgag gaggtagtat
    72841 tttcggatag gaaatagctt gattgttcgc aaagaaacat taaaaaaacc aatgctgaat
    72901 gtctatctcc aaaataaaat atctgggatt catattatga atacggctgt cagcggcaat
    72961 aacagtcaag ctctacgaga acgctttgcc aaagatgtac ttagctatac agcagataaa
    73021 gtatttattc tcattgggac gaatgatcta gcagagcata aacaactttc taaagaaacg
    73081 taccaaaaaa tctgcagtgg atgatagttc agctgaaaaa agactatagt tctgatcatg
    73141 ctttctttat cacgccacca gcagtcgatg agacaaaaca aaaatatcgg aataacaagt
    73201 tgatcaaaga atatatctct gttctcacag aagtagcaaa acgccatgat tgtgaagtat
    73261 cgatttttat acggaatttc ttgaacaaat ggattcgaag aatttttata atgggctaat
    73321 ggatgacgga cttcatttcg gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg
    73381 ttatatttaa agaatatttt gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg
    73441 gaggaatata aaacatgtta gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag
    73501 ttactgataa gtcacataat gaacgcattc cttgggacca gtactttatg gctcaggcag
    73561 tactgttatc tttacgtagt acgtgtacac gtttagaagt aggagcgaca ctcgttcgag
    73621 aaaaaaggat cattgctggc ggctataatg gagcagtcag tggagatgtc cactgcatag
    73681 atgaagggtg ttacgtcgtg gatggacact gcttgcgaac gatccatgca gagatgaatg
    73741 cactcctaca gtgtgcaaaa cttggtatcc cgacggaagg ttcagaaatc tatgtcacac
    73801 attttccatg tttggcctgt acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt
    73861 atctccatga ctatcgaaat gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat
    73921 ctgtacacca agttgcttta gactcacagc atttttcaaa acttcaaaat gaattaacag
    73981 gaaaacatgt ctagcgtttt agaaaggcat gttcgaaatc ggttgatttt tccggcaatt
    74041 acgtcgatga ttatctgttt gttgtattac actgacaatt ggggttatcg tttgcttctt
    74101 gccggaattt ttttatggtt ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt
    74161 ttgtgtagca taacggtatc catttcttat aggctacaag agtattcatt tttagaggaa
    74221 aaaacaacat ctgcgcaaat tactgtattt acagatacga ttcgtgcaaa tggtaatttt
    74281 ttgacaatgg aaggaaggtt atcagagaat aaacaaaagc tccagctttc ttatcaagta
    74341 aacacagaag cagaattgaa aacatggctt aacttccgag gaaagggaat agaattgact
    74401 gctgaaggcg agttcatctc tccagcaaaa aagcggaatc tttataattt tgaccaagaa
    74461 gcttacttca ggaaccatag aatttctgga cggttccaaa ttcaaaagat tgaacagatc
    74521 accatgcaaa atcactggcg aaactggctt cagagaaaaa gaggatcttt tatctccttt
    74581 gtccaatctg aatttccgca aaaaacaagt gtatatatta attctttatt atggggctat
    74641 aaagatgcag gattcagaga accagaagaa atttttaggg agagtggttt gctgcatctg
    74701 ttcagtctat cgggtctaca catccaattt tatttagggt ggctgtatta tttatttcga
    74761 cgtatcggat ggccactaca cgtcagtatt attccgttga gtctactaac tatctgttat
    74821 gtttctattg caggaagttc tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga
    74881 ttgctagtca aattgctgaa taagcattat tccaccatgg acttatttgc agttgtcttt
    74941 tggcttcttt tactatttga tcctcgattt ctttttcatg cgggtggaca gcttagtttt
    75001 ttcatgagtt ttttgtttat cctgatttct tttgatggag attggttgaa aaaaacggcc
    75061 tctcacgtcc tgtttactat gatcacgatg ccgttgattg tatggcattt ttatgagtgg
    75121 cctattttag gtagtctttt tacattactg attgcaccgg tattcaagtt tctttttctt
    75181 cctggtgtgt tatttctcac actcttcaat cgctggctgc cggaaacttt acttttgttg
    75241 atagaagagg gattaatctt atttgagcga ataatcgatt tttcttcagg gtcgtctctc
    75301 attataggac gtttgcccct tgtgctatct gggatgtata ttttcggatt actgatttgt
    75361 atggataggt tgaaagaaca acctgtgaaa cacttgtttt atgttggtct tttttctttt
    75421 atttttttga ctttgccatt tttacgcttt tcttcaacga tcgcttttat cgatgtcggt
    75481 caaggagaca gtatcttttt gcaaagtcct tttcgaaaag aaacgatttt gatcgatact
    75541 ggaggtcagt tacactttga aagagaaaag tgggcgcaag gtatgatacg tcctcctgct
    75601 gaaagaaata tcgttccata tctaaaaggt caggggataa gtgtaatcga taaactcatc
    75661 ttgacacacg acgatacgga tcatgtggga gaattaccca cgttatctca gcattttact
    75721 atcaaaaaaa tttatatcgg ttggggcgct ggacgaaatg agccattaca aaggcattta
    75781 tctgaagctc aaaaaaatgg tactgagatc attgaaatca aacatgggga caggatcagc
    75841 ggttattatg atctttatgt attgactcct tttgaaaagg gagagggaag aaatgaagat
    75901 tcgattggtc tatggatgga atataacaat aggcgctttt tatttttagg agatttgaat
    75961 caagcaatgg aaaaacaatt gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa
    76021 ttaggtcatc acggtagccg tacgtcaagt aacacagatt ttctttccca tattgaagca
    76081 aaacatggga ttatatcctg cggcgtaaat aatcgttatg gtcacccgca cagcgaagta
    76141 ttggagacac tagaagaaaa tcagataaaa acatggcgaa cggatcaaca aggaatgatc
    76201 acctatcgct ggcatccagt ttttcatcct catgggctag tggaaacaat gatagactag
    76261 taattctcat ctgctcatgg taggataatt tttgaaagtg gtgagtaatt tgagtataca
    76321 agcagagtta caaaaaatac gtaaagaaaa acttgcatct tgttacttga ttttaggaac
    76381 agaaaagttt ctccaagatc aagttcggac agaaattgtc aaaaaaatcc aattagaggg
    76441 cgaagatgac ttaaattttt taagttttga catggaaaat gcaagtttag atgaggtagt
    76501 agctgaagca gagacgttgc cgtttttcgg agatcaacgg ttagtattcg tagagaatcc
    76561 ttatttttta acaggtgaaa aagtaaacaa tggaatcgaa caaaatactg atttattgac
    76621 ggcttatttg aaagaaccat tagaatcgac tgtattagta ttctttgcgc catatgaaaa
    76681 gttggatgag cggaaaaaag taacgaaaca attgaaaaaa acagcaatta tcatcaatgt
    76741 ccaacagttg aatgaaaaag aagtacgtca atatctgatg aataccttag agaattcctc
    76801 aatcaaaatg gaacgaagtg caattgattt attcttgagg ttgaccgact tagatttatc
    76861 caaaatgatg agagagttac agaaattggt cctttatgga caaaatcaac agcaaatcac
    76921 ggtaagagaa gtagaagaat tagttcctaa aacgttagaa cataatattt ttgacatgac
    76981 ccaatatatt ttaaaaggga agacggaaca agctttgcgt ttatacgaag atttagttat
    77041 gcaaggggaa gaaacaatca aaattaatgc gattttgctt tctcaattac gtttattttt
    77101 acagacaaaa tttcttgtaa agattggtta tcagcaggca aatattgctg aaacattgaa
    77161 aatccatcca tatcgtgtaa aacttgccat gcaggaagta aaaaaattcg atgaacattt
    77221 gctggtacag ttgtttgatc aattagttga gatggattat caaatcaaaa cgggacaaat
    77281 cgaaaaagaa ttaagttttc aactattcgt tttgcgcaca gggcagttgt taagaaatcg
    77341 ataagaagaa agacgagggc aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg
    77401 acaaataaaa aaatcggccg aagccgattc ttttatttag cgattttttt gcttaaacga
    77461 gatttgtcgc ggttagcttt gtttttgtgg attagacctt ttgattcagc catatcaata
    77521 gctttaacag cttctttata taacgcatct acgttgtcag cacctgaagc tacagcatct
    77581 tcaaattttt tgattgcagt acgcatagcg cttgtttgag atgaattttt cacattagcg
    77641 ttttcgcttg tacgaacacg tttgattgca gattcaatgt ttggcattag tttcacctcc
    77701 gataacttaa gttcttacgc gtgttaccgc ataattcaac atttattatt atacctaaag
    77761 gcagcgtgct ttgcaatatg aacatgacaa gtttttttcc ttaacagtct tttattagga
    77821 ttttttttca gtaaaaacgg atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg
    77881 gaggcaatag cgtcaaacca aactagattt ttatttatct atttaaacaa agtttgaatg
    77941 ggaatgtttg gtactaagtt attatataac aaagagaacc gtttgcaaag ttttttttga
    78001 aaagggatac gattccctgt tgttctttgt ttttctatta agaaactgca gttattttat
    78061 aaccagtatc ctttgatcag taaaggctat tctggtgtac cgattatttt aatgtattca
    78121 tcgatatttt ttatcaacat tttttttatg accaactggt tccgacggtt ccatttgcca
    78181 cttacttcca gcacatattt atttaggggc aaacgtaaga tatattttgc caaatagcga
    78241 tctttaagca ggccattgat cacacggtcc tcaagctgca agttgatccc agtcagcatc
    78301 attggctgtt cttgtaaaac gtacgtatca attaaaagac cttcaaaagt ttccacaagc
    78361 gtcttccttt cttttcaaag taacctgaaa acaggcacta attcttccat tttgatattc
    78421 gttgaagtgg atgcgatcat tctttctttt gtccactgta atctagtata acaaaaaaga
    78481 gcagaagagc caaaaagaaa aaagagcatc gaattctata ggaagtctca ttggcacggc
    78541 ctaaattatt acaaaacatt gattaaaaaa agcccctgca tcgcaggagc atcccctata
    78601 tcgttttaat aggtcaatca aaaagcagta tcaaacacat tttgtatcaa atcaggaaat
    78661 aatagataag ctaagcccag aatcaaaagt accgctagaa gaaaccagcc gattgttcct
    78721 atgaaatact ttaatttttc gaacattgta tcacctcgtt gtcagtatat cacggatcag
    78781 taaattgggg gtgttaagaa tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc
    78841 aaatacttgt aagatacagt aagtgatacc agaagcaatg accggtccgg cggcgattcc
    78901 tcttaagaag acaacaccta aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc
    78961 agaagcaatc aatcctacac ccttggaaga taagacagcc actagcccac cgcaaaaaac
    79021 agctacccag ccaatagggg atttaaaggc ttctaataga tcacgtagac cgatttctcc
    79081 tgttgcgata gggattagaa tagccgctgt gatgatcgtc accccaatat tgattccttt
    79141 gccttgaagc caagggaaaa aggaacggga ggctggtatg agcttcataa tcaaaacaaa
    79201 agctgccgct atcatcagac tttgattttt agctaaaatt gctacaagta aaataattcc
    79261 taaaaaaagc caactttcca tgaaatatat tcctcacttt cttcgaaaca atcataacat
    79321 gtttttagaa agttacaaaa gtaggaattc atccttcaag acctattcat aagctgtttt
    79381 tttcataaaa taaacaaaat cataaaaaaa cactttgctt atttgacaga agacagccgc
    79441 gctttgtata gtggacaaga cttacatttt tttgaagaat cagaaagatc aggtaaaggt
    79501 cttagtaaac gcaagccatt cagaataacc aatattgtgc ttccttcgtg tcctacaact
    79561 ccaagaggta gatttaaaaa ttgcaggaag ttagaagcaa tcaacaagag aataacaaga
    79621 atcgaaaaaa taatattttg tttgatgatc cgccgcattt ttaaagatag ccgatgactg
    79681 tagacaaatt tatttaagtc gcttttcatt aagacgatgt ctgcgatatc catagcggca
    79741 tctgtccctt gccccatagc gacacctaaa gaagcggctg ctaaagcagg cgcatcattg
    79801 actccatcac caaccatcgc atttacttgg taaagttcat tctctttttt gactagttgt
    79861 gttttttctt ccggagtaca gcttgcatag taatgttcaa tccctacttg ttcagcaatg
    79921 gcctttgctg tgccagaatg gtcgccggtt atcatacttg tatggatgtt ttgggattta
    79981 aagtaggaaa ttacttgttg ggtatttgct tttggaatat ctagcagacc taaaacagca
    80041 attaactgct gatctttaga aagataaatc acggttttgc cttgattttc caaacgttcg
    80101 atcatttccg caatctcttt gctgattatc atcgtctcag gatcatatgc gtgcttgcca
    80161 actttccatt gcgcacctaa ataggtagtt tgtaatccaa aaccagtaat ttcttctatt
    80221 ggtaagtatt ggatttcttc aggtattgtg caatcgaaat attgcaaaat cgcatgagca
    80281 agtggatgcg tcgttttttg ttccatagca acaagaagtt gttgtgccaa atccttttca
    80341 tcaaagaaaa agctgtcagt tacaactggt tttcctttag tgagcgttcc tgttttatca
    80401 aaggcaatcg cttttaattc agaaagcttt tctaaatgga taccgccttt gattaaaatc
    80461 ccattttttg cagaatttga aagagcagct agagaagctg gtgtagcaga tgcgacaagc
    80521 gcacaaggag aagccacaac taataatacc attcctcgat aaaagctttc atcccaactc
    80581 caacctagga aaaagtgggg gagaaggatc attagaggaa cagcaagcaa aatcaatttt
    80641 acataattat tttcgattcg ttcgataaat gaagctgttt gagttggcgt attctgcgct
    80701 tcttctacta attggataat cttagcaaag acagtttcgg aactcgtttt tgttactcga
    80761 atcgtgatgg gatttccttg attcagcgtt cctgcataca tctcatcgcc taaatatttt
    80821 tctacaggca cagattcacc gcttaatgca gactcatcga tcgttgcctg tgtgctttct
    80881 aaaaagccat ctatcggaac agctgctcct ttagcgacaa ataccaagtt atccaccttt
    80941 agttgagaaa catctacttc ttgcatttgt ccattttctt ttaataattg ggctttttgt
    81001 ggttgaagat tcatcaaagc agtaatttct ttttgacttt tattagttgt gtattcctct
    81061 aatgcaccgc ttaaacaaaa gatgaatgtc aacattgctc cttcaaagta attaccgatc
    81121 aaacaggcac caatagcagc taaagccatc aataaatcca cattcagtgt ccgatcattc
    81181 cacaattctt tcaagccttc cgatgtttgt ttatatccac cgatgataat ggcactgata
    81241 taaaaaagtg gggtgatggt aagagagaat cggtcgaaga taaaaccgaa gatcataaat
    81301 aaaaggcagc cgattgttgc tgcaaaagct tttttctgat tagtcatcgt tattagacct
    81361 ccttgtcaaa acaaggatag cataataaaa atgtaattga taataataac agctaaatga
    81421 gaacgataat ggttatcatt tggtgattaa gcttgatgtt gtttttatta tttataatga
    81481 ttattattaa agaaaataaa aaaccagaac atttctcaaa taagagaagt gttct
//
LOCUS       DAFEPQ010000007        68781 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.007, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000007
VERSION     DAFEPQ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.007"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            233..475
                     /locus_tag="LP543_000507"
     CDS             233..475
                     /locus_tag="LP543_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBL2320699.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            472..942
                     /locus_tag="LP543_000508"
     CDS             472..942
                     /locus_tag="LP543_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBL2320700.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            1070..2284
                     /locus_tag="LP543_000509"
     CDS             1070..2284
                     /locus_tag="LP543_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBL2320701.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            3273..3482
                     /locus_tag="LP543_000510"
     CDS             3273..3482
                     /locus_tag="LP543_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HBL2320702.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            3594..5207
                     /locus_tag="LP543_000511"
     CDS             3594..5207
                     /locus_tag="LP543_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2320703.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            5242..6453
                     /locus_tag="LP543_000512"
                     /pseudo
     CDS             5242..6453
                     /locus_tag="LP543_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            6476..7402
                     /gene="glsA"
                     /locus_tag="LP543_000513"
     CDS             6476..7402
                     /gene="glsA"
                     /locus_tag="LP543_000513"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBL2320704.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            7610..9070
                     /locus_tag="LP543_000514"
     CDS             7610..9070
                     /locus_tag="LP543_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320705.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            9291..11054
                     /locus_tag="LP543_000515"
     CDS             9291..11054
                     /locus_tag="LP543_000515"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBL2320706.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            11138..11596
                     /locus_tag="LP543_000516"
     CDS             11138..11596
                     /locus_tag="LP543_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBL2320707.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            11705..12388
                     /locus_tag="LP543_000517"
     CDS             11705..12388
                     /locus_tag="LP543_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBL2320708.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            12535..13149
                     /locus_tag="LP543_000518"
     CDS             12535..13149
                     /locus_tag="LP543_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBL2320709.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            13165..13371
                     /locus_tag="LP543_000519"
     CDS             13165..13371
                     /locus_tag="LP543_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320710.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            13912..15042
                     /locus_tag="LP543_000520"
     CDS             13912..15042
                     /locus_tag="LP543_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL2320711.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            15134..15889
                     /locus_tag="LP543_000521"
     CDS             15134..15889
                     /locus_tag="LP543_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL2320712.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            15947..17986
                     /locus_tag="LP543_000522"
     CDS             15947..17986
                     /locus_tag="LP543_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBL2320713.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(18095..19246)
                     /locus_tag="LP543_000523"
     CDS             complement(18095..19246)
                     /locus_tag="LP543_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBL2320714.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(19400..19930)
                     /locus_tag="LP543_000524"
     CDS             complement(19400..19930)
                     /locus_tag="LP543_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBL2320715.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(20029..20379)
                     /locus_tag="LP543_000525"
     CDS             complement(20029..20379)
                     /locus_tag="LP543_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBL2320716.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(20536..22065)
                     /gene="ahpF"
                     /locus_tag="LP543_000526"
     CDS             complement(20536..22065)
                     /gene="ahpF"
                     /locus_tag="LP543_000526"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBL2320717.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(22075..22638)
                     /gene="ahpC"
                     /locus_tag="LP543_000527"
     CDS             complement(22075..22638)
                     /gene="ahpC"
                     /locus_tag="LP543_000527"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBL2320718.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(22782..23444)
                     /locus_tag="LP543_000528"
     CDS             complement(22782..23444)
                     /locus_tag="LP543_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2320719.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            23628..25274
                     /locus_tag="LP543_000529"
     CDS             23628..25274
                     /locus_tag="LP543_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBL2320720.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            25265..26347
                     /gene="uxuA"
                     /locus_tag="LP543_000530"
     CDS             25265..26347
                     /gene="uxuA"
                     /locus_tag="LP543_000530"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBL2320721.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(26455..28008)
                     /locus_tag="LP543_000531"
                     /pseudo
     CDS             complement(26455..28008)
                     /locus_tag="LP543_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            28156..29297
                     /locus_tag="LP543_000532"
                     /pseudo
     CDS             28156..29297
                     /locus_tag="LP543_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(29423..30502)
                     /locus_tag="LP543_000533"
     CDS             complement(29423..30502)
                     /locus_tag="LP543_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL2320722.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(30605..31045)
                     /locus_tag="LP543_000534"
     CDS             complement(30605..31045)
                     /locus_tag="LP543_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL2320723.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(31102..31512)
                     /locus_tag="LP543_000535"
     CDS             complement(31102..31512)
                     /locus_tag="LP543_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBL2320724.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(31692..32126)
                     /locus_tag="LP543_000536"
     CDS             complement(31692..32126)
                     /locus_tag="LP543_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL2320725.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(32226..33074)
                     /locus_tag="LP543_000537"
     CDS             complement(32226..33074)
                     /locus_tag="LP543_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL2320726.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            33463..35229
                     /locus_tag="LP543_000538"
     CDS             33463..35229
                     /locus_tag="LP543_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBL2320727.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            35331..36590
                     /locus_tag="LP543_000539"
     CDS             35331..36590
                     /locus_tag="LP543_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL2320728.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            36737..38038
                     /locus_tag="LP543_000540"
     CDS             36737..38038
                     /locus_tag="LP543_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2320729.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            38041..38892
                     /locus_tag="LP543_000541"
     CDS             38041..38892
                     /locus_tag="LP543_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2320730.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            38905..39720
                     /locus_tag="LP543_000542"
     CDS             38905..39720
                     /locus_tag="LP543_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBL2320731.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            39871..41037
                     /locus_tag="LP543_000543"
     CDS             39871..41037
                     /locus_tag="LP543_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL2320732.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPTQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            41267..41914
                     /locus_tag="LP543_000544"
     CDS             41267..41914
                     /locus_tag="LP543_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBL2320733.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            41998..42942
                     /locus_tag="LP543_000545"
     CDS             41998..42942
                     /locus_tag="LP543_000545"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBL2320734.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            43077..44528
                     /locus_tag="LP543_000546"
     CDS             43077..44528
                     /locus_tag="LP543_000546"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBL2320735.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            44549..45385
                     /locus_tag="LP543_000547"
     CDS             44549..45385
                     /locus_tag="LP543_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBL2320736.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            45385..46044
                     /locus_tag="LP543_000548"
     CDS             45385..46044
                     /locus_tag="LP543_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL2320737.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            46170..46859
                     /locus_tag="LP543_000549"
     CDS             46170..46859
                     /locus_tag="LP543_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320738.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            46865..47821
                     /locus_tag="LP543_000550"
     CDS             46865..47821
                     /locus_tag="LP543_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBL2320739.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            47865..48377
                     /locus_tag="LP543_000551"
     CDS             47865..48377
                     /locus_tag="LP543_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2320740.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(48633..49181)
                     /locus_tag="LP543_000552"
     CDS             complement(48633..49181)
                     /locus_tag="LP543_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL2320741.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(49270..50451)
                     /locus_tag="LP543_000553"
     CDS             complement(49270..50451)
                     /locus_tag="LP543_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBL2320742.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(50687..52195)
                     /locus_tag="LP543_000554"
     CDS             complement(50687..52195)
                     /locus_tag="LP543_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBL2320743.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            52354..52545
                     /locus_tag="LP543_000555"
     CDS             52354..52545
                     /locus_tag="LP543_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320744.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            52748..53665
                     /locus_tag="LP543_000556"
     CDS             52748..53665
                     /locus_tag="LP543_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBL2320745.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            53658..54731
                     /locus_tag="LP543_000557"
     CDS             53658..54731
                     /locus_tag="LP543_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320746.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            54879..55397
                     /locus_tag="LP543_000558"
     CDS             54879..55397
                     /locus_tag="LP543_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320747.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            55378..55770
                     /locus_tag="LP543_000559"
     CDS             55378..55770
                     /locus_tag="LP543_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBL2320748.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            55846..56781
                     /locus_tag="LP543_000560"
     CDS             55846..56781
                     /locus_tag="LP543_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBL2320749.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            56914..57693
                     /locus_tag="LP543_000561"
     CDS             56914..57693
                     /locus_tag="LP543_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBL2320750.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            57686..58003
                     /locus_tag="LP543_000562"
     CDS             57686..58003
                     /locus_tag="LP543_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBL2320751.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            58247..59857
                     /locus_tag="LP543_000563"
     CDS             58247..59857
                     /locus_tag="LP543_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL2320752.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            60014..60862
                     /locus_tag="LP543_000564"
     CDS             60014..60862
                     /locus_tag="LP543_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL2320753.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            61093..61572
                     /locus_tag="LP543_000565"
     CDS             61093..61572
                     /locus_tag="LP543_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320754.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            61605..63833
                     /locus_tag="LP543_000566"
     CDS             61605..63833
                     /locus_tag="LP543_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL2320755.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            63962..65605
                     /locus_tag="LP543_000567"
     CDS             63962..65605
                     /locus_tag="LP543_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBL2320756.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(65678..66223)
                     /locus_tag="LP543_000568"
     CDS             complement(65678..66223)
                     /locus_tag="LP543_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBL2320757.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(66246..66731)
                     /locus_tag="LP543_000569"
     CDS             complement(66246..66731)
                     /locus_tag="LP543_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL2320758.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(66721..67533)
                     /locus_tag="LP543_000570"
     CDS             complement(66721..67533)
                     /locus_tag="LP543_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBL2320759.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(67672..>68781)
                     /locus_tag="LP543_000571"
     CDS             complement(67672..>68781)
                     /locus_tag="LP543_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBL2320760.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        23016 a        12149 c        13517 g        20099 t
ORIGIN      
        1 agaaattgat tgtcgttttc ttttgcagaa atttaagtgc aggcaattat ggtagttgtc
       61 gtaattgctg tcagctcagt gtttatcact gatggtgtga aagatttacg gttggagatg
      121 ttcaataaga ttattggtgc ttaattattt ttattgtagt gctgatatga gaatgttata
      181 attggggtat aaaacataca aataaatttg ctgaaaaatg aggtatcaaa atatggaaaa
      241 tgaattactg gaaaacttag gaagacttca cacaactgaa cttggcgtta ttagaattaa
      301 aaagaactta tctttgaatg tagaaaatgt aattgaatgg tgcaaagaaa aaattagcct
      361 ttctaatgcg gaaatcacaa gaaaaggaaa aaactggtat ataacgattg ataattgtat
      421 tataacggta aatgcgtaca gttataccgt tatcactgct cacaaggtaa aatgataaat
      481 ttttctataa aggaattaaa cgcatttgca gttattgggc aggaagtaga actgactaat
      541 tatcaaaaaa agaatattca gattagtaca cagttttgga gaaagtttaa cagtagtttg
      601 aaaaaagcgt atctttcaca atcaggaaat tgggtaaaat atgcttttat ggaaagaaga
      661 aatggaaaac tttattattt ctgttctatt ccccaaagaa ccattacccc agagggcttt
      721 ctgtataaag aaataccgtc ttataaatat ttggttgtgg agcatattgg tgctatggat
      781 aaaatatatg aaacttatgg caagatttat gaagaaatta ttcctagtac atcgtatatt
      841 cctataaaag atattatcct acactttgaa aaatacgatt atcgttttca ttggaatagt
      901 gataattcag ttattgaaat ttggatacct attcaagatt agaagcaatt ttatatccat
      961 acacacaaag cagttagtct taggattgac tgctttttct atacagattt ttaatgacag
     1021 gcaattatca attatggtag ttgccttttt tgattggagg aattgaagaa tgaatgataa
     1081 aaactttatt gaagaactaa gacaaaagcg tgaggaatac ggagtaacac aaacaaggct
     1141 tgctgttgcc tgtggtatca gccgtgaata ttacaaccgc attgaaaaag gaaaacagcc
     1201 attgaatgac gaattaagag aaatcataga aaaacagatt gagcgtttca atccgcaaga
     1261 accactattc ttattgattg attactttcg tgtccgcttt cctactacgg acgcattggc
     1321 gattatccgt gatgtattac aactgaaatc taattacatg ctttatgaag attatggaaa
     1381 atacggatat gaaagcaaat atgtacttgg cgacatcaat atcatgtgtt ccatgcagga
     1441 gcatttaggt gttttattgg aactgaaagg caaaggctgt cggcaaatgg aatgttatct
     1501 gctggcacag gaacgctcat ggtatgactt catgctggat tgtatgacgg ctggtggtgt
     1561 gatgaaacgg cttgatttgg ctatcaatga taaagcagga atattggata ttccaaagtt
     1621 aaaggaaaaa tacaaggctg gcgaatgtat gtcctacttc cgtatgcaga aagattacag
     1681 cggtacagaa aaatgcggta gcgatttacc aaagaataca ggagaaacct tatatctcgg
     1741 ttcaacaagt agcgaattat atatgtgtgc ttatcagaaa aattatgagc agtatgtcaa
     1801 gaatggcaca gaaattgaag atacggagat taagaaccgt tttgaaatac gcatgaagaa
     1861 cgaacgagcc tattatgcgg ttgtagattt actgacctat cgggacgctg aacgcaccgc
     1921 tttttctatc atcaatcatt atgtccgctt tgttgacaga gaggacgaca agccaaaaag
     1981 tcaatggata acaaatgatg attgggcatg gtttgtaggg gaaaacagag agccgatacg
     2041 cttaaccaca aagccagagc cttacacttt acaaaaagcg ttgcattggc tacaaagaca
     2101 agtcgcacca accataaaaa tggtacaggc attagacaga gaaaatcgta caacgatact
     2161 gaaagatatg attgagcagg cagaactaaa agacaagcat aaacatttat tacaattaga
     2221 gaaatcaacc atagaagaac gtatagatac cgctgttcca caagagaatg acggtatttt
     2281 ttaatgtaat tgaaaaattt cagatttagt cagcaaagag ctgttttcat tccctatgtg
     2341 aagtgaaaga agaaaaataa attttttatt aaatctgcaa caaaatggct acttgcgtac
     2401 agatatggtg cgagaaaagg aaatctaaac tttttttgaa aataggtcat taaatcgggg
     2461 cttgctgtcc ctatatgaag tgaaagaaga aaaaagtttt gaaaaaatga cggaaattcg
     2521 tgattagtgg acagtcttct gtgaaaagag gaaaaacatc atcattttgt attcaaacag
     2581 gcattagcag ttgagtgttg atgtgaaaag atgaaaatac tttgttctca acttaacaat
     2641 ttaaggtttc cgttccctat atggtgcaaa gaaaatattt cattctattg ttggcagtaa
     2701 cggtatggtt tgggtagtta gggtgtgaaa aggaaaatat tcttttttca tcatcaactt
     2761 agcaaaatga cattttctat ccctatatgg tgcatgaaaa gaagttaaaa tttttccatt
     2821 taaacttagc aaatggacgt ttgctgtaca gatagtagtg aaaaaggaga aaatcaaccg
     2881 ttgattgcca aaatgaaccg ttccatttgt actaataatg gaaaaagttt tggattttgg
     2941 ttacgtttct cctcttttcc aacgcgatat ataggaaaga cgattttttt ctttcaatcg
     3001 ttctttgaca actgaataaa gctattcaat acgtttgaca gacagctagc cgttgaaaca
     3061 aatacgccac gacctttccc ctgcaagagc gagcgaaaac ctatttgtgt tcttgtaaaa
     3121 gatttgtcct cttttatcgc catgacctgt actgtcagat aatgatactc ccgtacagcc
     3181 acagcttgag cattaagagc gtcgcacgca atgggtacgg ctacataaga actatgcaga
     3241 ggtggaactc ctgtgggcta tgacaaaagc cgttgaattg cttagaaaga ttttacagaa
     3301 agggggtatg tgttattgat aatgctgtaa aaacaattca aaagaaaaat ggcaagtgtg
     3361 gcattggcaa cagaggaaag acaaagattg tagtaaaaga gcatttttcc gaaaagggca
     3421 aaacaatgga agaacttcta actgatgtca tgctggaaaa agcaaagcag acaatcgcat
     3481 aaaatcggtg cagttgtaag aaatagattg aatgacaaaa agtacccgtg ttatacttta
     3541 cttgcaagca ggtattgtaa acacgggtta gttataaagg aggataactg acaatgaaac
     3601 aacagattta caatactgca ctttatctta ggttaagccg agatgatgaa ttacagggcg
     3661 aaagttccag cattaccaca cagagaagta tgttgcgttt atatgcaaaa gaacatcatt
     3721 tgaatgtcat tgatgaatat attgatgacg gctggtcggg aacaaatttt gacagaccga
     3781 gttttcaaag aatgattgag gatatagagg caggaaaaat caactgtgtt gtaacgaaag
     3841 acctttcccg tcttggcaga aattatatta tgacaggaca atatacagaa ttgtatttcc
     3901 ctagtcataa tgtccgctac atagcgattg atgacggtgt agacagcgaa aaaggcgaaa
     3961 gtgagattgc accatttaag aacatcatca atgaatgggt ggcaagagat acaagccgta
     4021 aagtcaaatc agcatttaag acaaagtttg cggagggggc tcattacggt gcttatgctc
     4081 cgttaggata taagaaacac cctgacatca aaggaaaact gttggttgat gatgaaacaa
     4141 aatggattat tgaaaaaatc ttttccctag cctatcaagg ttacggaagt gcaaaaatca
     4201 caaagcagtt aagagcagaa aaagttccga cagcgtcatg gctaaatttt acaagatacg
     4261 gtacttttgc acatatcttt gagggaaaac ccgaaagtaa gcgttatgag tggacgattg
     4321 ctcatgtcaa ggcgatattg aaaagtgagg tttatatcgg aaacagtgtt cacaatatgc
     4381 agtctacggt atcgttcaag agcaaaaaga aagtgcgtaa gcccgaaagt gaatggtttc
     4441 gagtagaaaa cactcacgag ccgattattg acaaggaagt gttctatcgt gtgcaggagc
     4501 agataaaatc aagacgcaga cagacaaagg aaaaggcaac gccgattttt gcagggcttg
     4561 tcaagtgtgc ggattgtggt tggtctatga gatttggaac gaataagaca aataaaacgc
     4621 cttacagtta ttatgcttgc agttactacg ggcagtttgg caaaggtaat tgttctatgc
     4681 actacattcg ttatgatgtg ctgtatcaag ccgtattgga acgcttgcag tattgggcta
     4741 aggcagtaca gcaggacgaa gaaaaggtat tgaacaagat acagaaagct ggcaatgcag
     4801 agagaatacg ggaaaagaag aaaaaggcaa gtacattgaa gaaagccgag aacagacaaa
     4861 atgagattga ccgtttattt gcgaaaatgt atgaagatag agcctgtgag aagataacag
     4921 agcgaaattt tgtgatgttg tccagcaagt accaaaaaga acagatagaa ctggaacagc
     4981 agataacaag cctaagagaa gaactaagta aaatggaaca ggatatgata ggtgctgaaa
     5041 agtggattga gttaatcaag gaatattccg taccaaagga actgacagca ccattattaa
     5101 atgccatgat agaaaaaatc ctcatacatg aagcaacaac gaatgaggat aacgaaagaa
     5161 tacaggaaat tgagatatat taccgattta ttggaagagt tgagtaatca acaagagtaa
     5221 tatttttaac taagggaaac cggcggtgtc tatacttggg tcagtcgcac attgggaaaa
     5281 cgattcggtt ttgccgcaat ctttttccaa tggtttgaga taacagttgg ctacttgacc
     5341 atgctgtatt tcttaactgg tgcattatcc tatgcaacgg gtattggtgc tatacaaaac
     5401 aataaatatc tgaaattggc aatcctattg attattttct gggctatttt actctcacag
     5461 ttaaaaggaa ccaaatacac ttcattgatt gctcggatcg gttttgtagc tggtatcttg
     5521 ttgccagcat tagtcttgtt tggcttaggg atccattatg tcgcttcagg cgctccacta
     5581 caaacgatac tgtctatgaa aacattgatt cctgattttt cgaaattgcc tacgctggtt
     5641 gtttttgttt cctttatttt agcatatatg ggcgtagaaa catctgccag tcatgcaaat
     5701 gaaatggaaa accctaaaaa ggattatcca ttagcaatgt ttgtattggt tattctggca
     5761 atcattttag atacttttgg cggattgacc gtcgctacta cagtacctca acatggtttg
     5821 tctcttaata caggggtgat tcaatcgtta gaattcctat taagacacat tgatccaaat
     5881 cttgcatgga ttgcgaaaat tcttgcgatt cttgtttgtt taggtgtcat cggagaaatt
     5941 gcttcttggg taacctctcc ttcaaaagca cttcacgtag cagcagaaga aggattactt
     6001 cctgaatatt ttgcaaaaga aaacacacac ggtgttcctg ctcatttgat gatcgcaaat
     6061 gggatcgttg caacaatctg ggcagccgtc tttacattaa gcggcggggg gaataatctt
     6121 tcatttcttg cagcgatgtc attgactgtt gtaatctacc tgatgttgta ctttatgttt
     6181 ttcgcgggct atttgaagct ggtttttaaa gatgacagtc ttgaaagagc ctatcaggtt
     6241 ccaggcggaa aagttgtaaa agtaatcgtt gcttgtttag gtcttttgac ttcacttttt
     6301 gctttttgta tctcatttgt tccacctgct agtatcgcag ctggacagca tgggatttat
     6361 gaaacgattc tgattgtttc ttatcttatc gtcttagtta ttccatttac gatctacgca
     6421 tgccgtcata aattccagca tgcagaaaaa taaaaaatga aagctggtta ttcaaatgga
     6481 agtaaaagaa ttagaacaat tgattggaaa aaatttccac tattatcaaa caggacaagt
     6541 ggcggattac attccagcat tagctgaagt ggatcccaaa caattaggta tggcgattta
     6601 tgatttggat acaaaacaac tgattgaggg aggagacgcc gatgtgcgct ttgccatcga
     6661 aagcatgtca aaagttcccg tattgttatt agcaatcaaa gacaacggaa tagaaaaagt
     6721 ctttcaaaca atcaacacgg aaccaaccgg ttttgccttc aattctatca tgaatatgga
     6781 aatcaatcat cgcaaacatc caatgaatcc ttttgttaat gcaggagcga ttgcaacaac
     6841 ctcgttgatt gccggtaaag atgcagatga aaaatttgag cgaatcttgt catttatgaa
     6901 agagatctgt gatgatccta atatcacatt gaatgaagaa atctatcatt ctgaatcaag
     6961 aacaggtgat atcaatcgct ctcttgctta ctatatgaaa ggtaatcaaa tgatcgaagg
     7021 agacgtaccg gagatactcg atgcttattt cagacaatgc tccctcaatg tcaccgctac
     7081 aggcatagca aaattagctg ctgtgctggc aaataaagga attgctcctt ggaatggcaa
     7141 acaaatcatt actgaagaaa gtgctacaat cgtcaaatca attatgacaa ctgctggttt
     7201 gtatgatgaa tcaggagagt tttccgtcca tgttggtgta cctgcaaaaa gcggtgtagg
     7261 cggcgggttg atggcagctg tgccaaatcg ttacggcata ggtgtcttca gcccggcact
     7321 agatccattt ggtaacagcg cagctggtat ccaattgttg aaagatgtag tcaaagaact
     7381 agacgctgac atctttgaat aaccattctt gaaagaggct ggggtaaaag tccaaacggc
     7441 tcaaagctac ctacaatgag gctgaaattg tgagcttgtt tgacttttgt cccagtttct
     7501 ttttttattg gtttttgaaa gggcttttct aaataaaaag gaaacaaaaa tacgatttac
     7561 agatatgatt aaataaaatc ataagaaaaa ctggaggcgt gtatcaggaa tgctggaaaa
     7621 ttacatcgag cgaaatatat ttagaaaagt gtatctgtgt gaacaattat ttgaattcca
     7681 agaaatagac atcgaacaaa cagctatttc tctaagggtg actacaccaa cgatccttca
     7741 cgatctagaa agtctagccg agtgtctaga atattgtata aaagaacaag taagagagaa
     7801 gcataagtat aaactcgtat tcaaacatgg tattgctctt tctgagctca cacagtttct
     7861 gtacggtcaa tcttattttt taaagttttt aagttattat tttaatggta tattcacgag
     7921 tacagaactg gctgatcttg aatttatttc tttgagtaaa gtatatacaa tcaaaaaaat
     7981 cgtattggat ttttttaaag aaaatgatta tttgaaaaat aaagaaatca ttatcccaga
     8041 atttgactcg cggaacatct tgctagcttt agtacgttat gtcaattggg aaggctatga
     8101 aaataaaaat ccgtatgtag aagaagcttg taacgagttg attgattatg tagagactca
     8161 tttttttaaa agaagatatt cggaagatga aaagtccttt atcatacgag aaatagagat
     8221 cgcaatcgga agaaaggcat ttccccttgg actgaaagaa gaagataaga aaagcgcaga
     8281 aacgaagccg ttatttcaat tagtctgtca aggattggaa gagcaaaaag gaattctaga
     8341 cttacaagaa gaagatgtct attatatttt ttctttgttc aacagtagaa attataccaa
     8401 tgagaacatg gagctgttaa gaaaagattt cgaagttgtt tataaaaatt ttgttctcaa
     8461 tgacctatca ctgaatgaat tgattttaca gattattagt atcgctgaaa tcgacgatgc
     8521 agaaaatttt attttcaagc aatccttttt acaatttgtc cgtacgttgt gggcagatgg
     8581 acaagtattt ttaccggaaa ggatctatct tctatcagaa aaagaacaac ttctgtacgg
     8641 aaaaattgta gaaatattgg aagagtggaa aaagaaaaat caaatccaac taagatggaa
     8701 tgaaaatttc attcgaaaat tcgtcaaaag tctttctttg atcaacagtg aacaagcggg
     8761 gagacaggaa atagaaattt ttgttgtttc agaaagttca gtcaaacaat ttttttatcg
     8821 aacccaattt catttgtatg cggaagaagg catcgctgaa ataaatccta ttttatttcg
     8881 ttcattagaa gaattgcctg atgaatgtct atgtgcgaaa aaaagagtgg ttttgtgcga
     8941 tgctgctagc tatcaaaatg gattagaaac tgaaaagaca aagatctatc caatctcatt
     9001 aaaaggtgca aacatccatt tgtatcatat tgctcaggaa cttcacctat taaaaaaaca
     9061 tatttcttga tgagacctta attaggagac cttctacaaa gctttttaat tgtctgtaga
     9121 aggtttttat ttgagtcctg attcgtgcga agatctactt atataacaag ctttctctga
     9181 aattttttta aaatgctatt gacttagagt caactctaag tagtagaata gataaagtgt
     9241 aacaaatcta ttttcattaa aagaagatag tacggaggag agaatgtatt atgagtacag
     9301 tattacatgt attggatgca atcaatagcc ccgcagatgt caaaaaatta tctgttgagg
     9361 aaatgaaaca gttggcagaa gagatgcgcc aaaaaatctt gcaaaaggac agcgatgtag
     9421 gtgggcacgt tggtccgaat ctaggtgtag tagaattaac gattgcgttt cattatgtat
     9481 ttgattcacc aaaggataaa atcgtttggg atgtttccca tcagtcttat ggtcataagc
     9541 ttttaactgg tcgaaaaaat ggttttgaaa aaggacatta ccgtgaagtg agcggatatt
     9601 ccaaccaaca tgaaagtaaa catgactatt ttacagtggg gcacacatct acatctatca
     9661 gcttagcaac aggtttagct aaagcgagag atctgaagaa agaatcagga aatgtggtag
     9721 catttatcgg tgacggctcc ctaagtggtg gcttagcttt tgaaggattg aacaatggtg
     9781 gtgttttaca atccaatcta atcgtattag taaacgataa tgagatgtct attgatgaga
     9841 atcatggcag tttatataaa aacttagctg agcttcgtca aaccaacggt aaaagcagca
     9901 ataacctatt taaagcaatg ggatttgatt atcgttacgt ggataatgga aacgatctgg
     9961 aaacggtgat ccgtttattt gaagaagtaa aagatattga tcatccgatt gtacttcacg
    10021 tccatacgga aaaaggtcgc ggttatcagc cggcaatcga taataaaatg aaatatcatt
    10081 ggcatgtacc atttgatctg tccaccgggg aggcaaagca agtaacttct ggagaaacat
    10141 ttaatatgat catcatggat tatttggata agaaaatgca aacaagtaca gctcctattg
    10201 ccgcagtcaa tgcagcgata ccaggcgcct ttggactgaa aacattccaa gaaaaacatc
    10261 cagatcatta ttttgacgtg gggatcgctg aacagcactc tatcactttc gcttctgctt
    10321 tggcttctca gggagtacgt ccagtcgtct tccaatcaag tactttcttg caacgggcat
    10381 atgatcaatt atcacatgat ttagcgatca acgagaatcc agtgattatt ttagtcggtg
    10441 gcggaacgat ctcagctagt gaccgaacac accaaggaac atttgacatt ccttacttat
    10501 caagtatccc gaacatcaaa tatcttgctc cagcaacaaa agaggatatg atctctatgc
    10561 tagattgggc gttcgaacaa acagcgcagc ctgtagcaat ccgaattcct acacatggtg
    10621 tagaacatgg tacagctatc gcaaatgatt atagtattcc agcctatcaa acagtaaata
    10681 atggaggaaa agtagcaatt ttagctttag gcggttttct tgaattagga aaagaaacag
    10741 taaaagcttt tagaaaaaaa ggaatcgaag caacgttgat caatccagga acgatcacag
    10801 aaaatgacgt agaaacatta gataaattaa aagaaacaca cgaattagtc atcacattag
    10861 aagatggtag tttgtcagga ggatttggcg aaaatatcag tcgttattat ggtgattctt
    10921 caatgaacgt attaaatttt ggtgcagaga aagaattcac ggatagcgtt cctgtagggc
    10981 agctttatga acgttatcat ttaacaccaa aacaaatcgt tgaagatagt ctgcgtgtct
    11041 tgaatattca ataattcatt atgctcacca tccagataat tgtttatact agtatctgga
    11101 tggttttttc attataaaaa gcaaaggggt ggatgttatg tatcagcaag tagaagaata
    11161 tgtcaaaaag ttccaagaaa aatatcctag ttatcaatca gaaaaattag gtttcttttc
    11221 atttggggat acagaaaaaa tggcagacga gttgagcgaa ctagttgtaa aaaagataaa
    11281 gactggtaca tcaagtggtt acgatctgta taaacaagag aatgagcctt taccaaaagt
    11341 tggtcaatta gacgtggtcc tgaatggaaa aaatgaacca gtttgtatca tcaaaaatat
    11401 aaatgtgacc atcttgcctt ttagtgaagt cacgaaagag catgcttgga aagaaggaga
    11461 aggggatcgg acattagctt attggcggaa agtccatgct gagtttttta aacatgctta
    11521 taaagaagcg ttgaacatag aatttacaga agagaagctg gtcgtatacg aagaatttga
    11581 agtaattttc ttttagtaag aaaggtctgc tgaaagaaat agctcattat tgtctcctga
    11641 atagaaaagg gttacatttt tatatgaagc tgtattttta tataacatta ctggaggaga
    11701 tattgtggat acactggaac aaatcaaagg cggagtcgtc gtttcttgtc aagctttgga
    11761 aaacgagccg ctacacagtt cttttattat ggggagaatg gctttggctg caaaagaagg
    11821 cggagcagtt ggtattcgag caaatacgac agaagatatc aatgaaatca agcgaatcgt
    11881 tgatttaccg gtaataggga ttatcaaaag agattacgat gggtcagaag tgtttattac
    11941 tgcgacagaa aaagaagtgg aagaactttt ggagacgcaa gcagaaatca ttgcactgga
    12001 tgcaacttct cgtaaacgtc cgacagaaaa aacaactgct gaacttgtcc aaatgattca
    12061 cgaacacggt cggttagcta tggcagatat ttcgactttt gaggaagcgc ttcaagcgca
    12121 agaagatggt tttgatcttt tgtctacgac tttagcaggc tatacagaat attcacgaaa
    12181 gacgcagaca cctgattttg atttattgaa tcaaatcata gaacaagtcg atatcccagt
    12241 aattatggaa ggacatactg actctcctga acaagtagag aaagcatatg aattaggtgc
    12301 tttttcagtc gtagttggat ctattatcac ccgtccgcaa ttgatcacta aaagatatgt
    12361 cgaagccgca agtaaagcaa ataactaaaa aacacgcacg gcacagattc ttgacaaaaa
    12421 agaaaaattg atagatagtt taaagcagat tctaagaaga tgaaaaaagc attacttttc
    12481 attttgtctt caagatctgc tttttaacga attttataga aagaaggcga aaccatgttt
    12541 gaacggagtc cttcagaatt gacagaacgt gaaaattaca agttgctgat tggttcagtc
    12601 atccctagac cagtagcagt agtaacgact aaatcagcaa aagatatagt gaatatcgca
    12661 cccttcagtt acttcaatat cgtcagttcg aatcgaccca tcttatcctt ggcgatccag
    12721 agaaaacaag atgcagtaaa agacacagca aagaatattc ttgagacaag agaagctgtt
    12781 atccatatcc ttgatgagga caacgtagaa gaagtaaata aaacagcagc caatctgcct
    12841 aatgaggaaa gcgaactgta cctgactgac ttgactttaa ctgattcagt aatcatttca
    12901 gtaccagcat tgacagaagc aaaagttcgt tttgaaacat ccttatatga acatgtcgag
    12961 atacggaata aaaataaagt aacagctgat ttatttttgc tagaagtaca gaaatatcat
    13021 ttaaatgaaa cagtctatga tgataagaca ggaaggatcg atccaagaaa attgaatgct
    13081 gtaagccgtt tggcaggaaa tgattatgca gcaataggag aaattttcac gattgcacgt
    13141 cccgaataaa aaggagcact agttatgtta aaaataagct ataaaccatc gacagattca
    13201 aaagaaatga aaaaagaata tgaaacagtg aatgattttc tgcaagggca gtacttagaa
    13261 gttccgccat tgcaagacca ttttgtcgtt actactgtta ctttagatgg taaggaaata
    13321 gaaatgccag atcaaacgat tagcggattg tttaattact ttaacaaata aatatttatt
    13381 ccgaaaagtg aaaaaacata taaaaaaaca cataaaacta aaaaattatc aaagaatcct
    13441 ggtgcaactg aaagaatttg caacgggatt ctttcaactt atttgttatt tttttctaaa
    13501 aaatattaaa gattgttaaa tttcactatt tcatttcaaa ttccattgcc taatcattag
    13561 gaaaagtcta tcatgaactc gtaaatagaa tatagtaata accttgttag gtgaggctcc
    13621 tatacaaaca taggctgttg cccagaaacg tcgaaagtcg ccaatgggta aaacagaaac
    13681 tgttggatta aggctttttc caatagctaa gaatgttttc tttacgttgt atagtgctaa
    13741 aactcggacg atgagatgat gctaggtcag aattcttgtt cgaaaaggtt tctgaccttt
    13801 tctttatatt tttgtgactg acttatttga tgagacaaac tcgctcctca cctgacaagt
    13861 ggattgctgt gtgaggagga gatgcggtga aaaaagatcg attggtggat tttgggtcct
    13921 tatttgactt tgtccatgat cggtctttta gaagtataca gtgccagttc ttatcgttta
    13981 ttgcaagccg atgaaaatac aaaaagtcta ttattgcgtc aactaatttt catatttttg
    14041 agttggagcg tgatcttctt agctcgttca gtcaaactac actatttact tcaccctaaa
    14101 atagcaggat acggtttagc cttatcgatt ttctttttag tattagtaag aatagggata
    14161 ttcggtgtca ctgtcaacgg cgcacaacgt tggatctctc tgtttggcat tcaattccag
    14221 ccttctgaac tggcaaatct ttttttgatt ttttatttaa gctggttttt tcgtgacgga
    14281 aatagtagcc caaaagatct aaaaaaacca ttcctgatta cagtaggtat aacttttctg
    14341 attttatttc agccaaagat tgctggagca ttgatgatcc tttcgattgc gtgggtcata
    14401 ttttgggcag cggcggttcc atttaaaaaa gggatctatc taatcgttac tttttctgca
    14461 ttgctgattg gagcagcagg cggggtatta tatttaggaa ataaaggttg gcttccacag
    14521 atgtttaatc atgcttatga aaggatcgcg actttaagag attcttttat cgatagtcat
    14581 ggtgcgggct atcaaatgac ccattcgttt tatgcactat ataatggtgg catatttggg
    14641 cggggattag gaaatagtat cacaaaaaaa gggtatcttc cagagacaga aacagatttt
    14701 attttttcga ttattacaga agagcttggg ctaatcggtg cattgtgtgt gcttttctta
    14761 ttgttctcat tatgcatgag aatcttttgt ttaagcagtc gctgtaaaaa tcagcaagct
    14821 ggattgtttc ttttaggatt cggaactttg ctattcgttc agacgataat gaacgtggga
    14881 agtatcgcgg ggctgatgcc aatgactggt gtgccattgc catttgttag ctacggagga
    14941 acaagttatc tgatcctttc tttaggaatc ggaatcacgt tgaatatttc ttcaaagata
    15001 caggccgaag agttggcgct ctatcgacct gaaaaacaat aaagagaaac tgagaaactc
    15061 tcagtttgaa ttatcataaa gtacaatctt ttatttttca agcgagaggt tcttttagaa
    15121 agaggaattc gcaatgaaag tatctcaaaa aatcatatta tctattttgt tactgcttac
    15181 attagtagtt ggctattttt cgattgagtt tatccatgga ttttcgtctg caaaacagag
    15241 ttcaactgtg aaaaaggtag agccgaaaag tatccctacc acactaaatg tggctttgat
    15301 tggttcagat gctcgttcga aagacgaaaa cggtcgctcg gattcacttc tggttgcaca
    15361 atacaaccag aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga
    15421 tattccaggt tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt
    15481 attaaatcaa acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac
    15541 atttcaagat tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc
    15601 agaaaaatct ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg
    15661 taatacttta ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat
    15721 cagaaggcag cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat
    15781 attcaagcta cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga
    15841 aagtgtgctt ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt
    15901 ataaatagtg aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga
    15961 caagcacggc aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc
    16021 tgcgggtggc ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg
    16081 agaaaagaca gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc
    16141 agaaatgaca tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga
    16201 taaataccag aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa
    16261 agtagataaa aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc
    16321 attaggtgaa ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac
    16381 cacgatcaac tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag
    16441 cctgaccaca caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc
    16501 aacaacaggc aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga
    16561 aaaaacagcc aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa
    16621 tcaggcgatt tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga
    16681 tggagcaacg ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta
    16741 tcctttgggt gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga
    16801 tattgataag aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat
    16861 ggcttttgat aaggatcttc gtgggacaac aggtgggaaa ttaagcatta cagatgcaga
    16921 cggtgtagag aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt
    16981 gacaatcgat gcgaaggcac aaaaaacagc ttttgacagt ctaggaggaa aagctggatc
    17041 aactgttgcg acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta
    17101 tgatccaaac aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa
    17161 tcctgaacat ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa
    17221 aatgattaca gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac
    17281 gatcaacggg cttaaatggc aaaaagacag ttcttgggga tcttatcaag caacgcgtgt
    17341 cagtagtgat gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata
    17401 tgcggcacaa gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt
    17461 catttttggt gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga
    17521 agatagcttt aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct
    17581 aattactcct atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt
    17641 ctatcctaaa ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac
    17701 agcagtacaa acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc
    17761 acattctctt tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa
    17821 agaaaaacag gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa
    17881 ccaaggttat atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa
    17941 acgagcatcg gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta
    18001 agtacagtaa aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc
    18061 acgatgtctc tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa
    18121 aatatttgag aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg
    18181 aatagtactg tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca
    18241 tttctccacg cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg
    18301 caatctgagc accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata
    18361 tagaaataaa gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga
    18421 caaaaaatac tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt
    18481 ctttaacctt tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa
    18541 gcccaacaag atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa
    18601 tagaagaatt tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga
    18661 ctaataagaa aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag
    18721 cattgctaga agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta
    18781 aaatgtcatc taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt
    18841 ttagttcttt taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa
    18901 agaaaatcgc ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa
    18961 ccgggaagat gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa
    19021 gagataaatc actttccaga ccagctaaaa acattaataa gatcacacca atctccgaaa
    19081 agtcatggac tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac
    19141 caacaagtaa ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa
    19201 tcgttgttga aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt
    19261 tgtataaaat atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata
    19321 ctcacaaaaa aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta
    19381 ttttaataac taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa
    19441 tctcctggat gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac
    19501 ttttgaatgg attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa
    19561 cactccaact tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga
    19621 taacagctaa tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga
    19681 cgatcagagc taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg
    19741 ttttcctttt aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg
    19801 cggtgatcag ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc
    19861 cagaaataat ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg
    19921 tctcaaacat ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca
    19981 aattaaatgt tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag
    20041 gatcttttcc atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat
    20101 ttctttcatc aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat
    20161 caaggaacgt gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg
    20221 tttttgtagt tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc
    20281 atctacttcc tcttttatac gatcttttct ctccgctttt gctacataca taggataaac
    20341 gcttgcaaaa cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt
    20401 tcaaattatt tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa
    20461 aaacaaaaag gattgtgaca aaattcttgt caccatcctt ttttccgctc tctgactttt
    20521 tacaatcttc atagctcaag tgcgcatcag ataatcgaat gctcctaaag ctgcagtagc
    20581 tcctgaaccc attgaaacaa tgatttgttt gtatggacta tctgtacaat caccagctgc
    20641 gaaaacgccg gggatattcg ttgcgccatt tttctcggtc actatttcat ttcttggatt
    20701 catttcgatc gttccatcta gccaatccgt attagggagt aagccgacta gaatgaatac
    20761 tcctgaaaca gctagtgtat gcatctccat cgtacggcga tcgatatagg agagagattc
    20821 tacctgattc gtaccgctga tttctttcgt cgctgcatgt ttgataacag tgacattcgc
    20881 aagagagact agtttatcct gtaagatttg atctgctttt aattctggta agaattctag
    20941 aacatataca tgttttgcta acccagccaa atcgattgct gcttcaacac cagaattacc
    21001 accaccgata acagcaatct cttttccttt aaataaaggg ccgtcacagt gtgggcaata
    21061 agcaattcct ttatttttaa actctttttc gcctggtaca ttgatagaac gccagtgcgc
    21121 acctgtcgcg atgatggcgg tttttgcttt aagtgatata ccgttagcta agccaatatt
    21181 aataaaatgt tgctctttat ttaaggaaac ggcctgttga ttcatcataa tgtcgatagg
    21241 gtaactacgc acatgacttt ctacttgctg catcaatttt ggaccttctg tgtaagaggt
    21301 accaatcata ttttcaatcc ctagtgtttc aacgacttgt ccgccgaaag aatcagcgac
    21361 aagtcctgta cggattcctt tacgtgctgc atagatagct gcactactag ctgctggtcc
    21421 accgccaatc actaatacat cataaggttc tttttcaaac aactcttctt caatcaaagg
    21481 gccagtgatt ttttcaacaa tttgttcgat cgtcatgcgt ccgctactaa attcttctcc
    21541 atttaaaaac acagttggca ctgccatgat ttttttatca tcgatttctt tttggaacat
    21601 cccaccttca atcatcgtat gagtaatatt tggatttaat acagctaata tattcaatgc
    21661 ctgtacaacg tcggggcaat tatgacaagt caagctaaca taactgtcaa aatgcaatgt
    21721 ttgtgttatt tttttgattt taagactcaa agattcactg attttcggtg cccgtccact
    21781 aatttgtaac atagccagta aaaaagagga aaattcatga ccgaggggga taccagcaaa
    21841 aacgattcca ctttctttgc caactggatt gatagaaaaa ctaggtgtgc gcatcgaatc
    21901 tcttttttcc caagagattt taggagacat ttctgctatt tccttcgtaa attcagctag
    21961 tttttgacta ttttcactat tatccaaact aacagaaaaa acaataggag attcaatcag
    22021 tgctaaatat tgttcgagat tttgtttagt ttctttgtct aacatatcca ccacctaaat
    22081 ctttccaaca agatcaaaac ttggtttcaa tgtttcgcca ctttctttcc attttgccgg
    22141 acaaacttca cctgggtggg tacgtacata ttgagcagcg cgaatcttat caatcagtgc
    22201 actagcgtca cgaccgattc catctgcatt gatttccata gcttgaacga ttccgtctgg
    22261 atcaataata aatgttcctc gctgagctaa tccttgctct tcatccaaaa catcaaaaat
    22321 acgagaaatc tgatgagacg gatcaccgat catcacgtat tcgattttac cgatagcatc
    22381 tgatgtatca tgccatgctt tgtgagtaaa gtgggtatcg gtagagcaag aaaagacttc
    22441 aacacctaaa gatttcaatg tactgtattg ttcttgtaag tcttctaatt cagtaggaca
    22501 aacaaaagta aaatcagctg gatagaagca aagaatactc cagttcccca taagatcttt
    22561 ttcactgact tctaaaaatt ctccttgatg ataagcatgt gcattaaatt cttcgatttt
    22621 tttcccaatc aatgacatag taaaacctcc aataataatt atttatttat aattattgta
    22681 atttagttgg agttgcaaag tcaatggata ttttgttatt taataaagat tactcaaaaa
    22741 cgaaggttaa ttataaaaat agaagaggtc gcaagaaaat gctactgctg gaaatactcg
    22801 ggataacgtt gagtaacagt ttcctgttct ttcagcatca tgtgtaagtg caatcccgtt
    22861 tgaaatcgag cttcttctgg ttttttatca actaaagcct tgagtaaatc ataatgttgt
    22921 tccatgatcg tttcccatgg aagcccctga atggtcactc gtaaccagcg gaaacgttct
    22981 aaatgggtat tatggtcatc taaccagttc caaacttctt ttcttcctgc aatttcgaaa
    23041 caagcttcat gaaataaatt atctgcacgg aaaaaagcgc gttcgtcatt tattttgact
    23101 gctttttctt gttcttctaa aatcgtttct agcactattc gtgtttgtgt gtccaatttg
    23161 gaacaacatt caatcatgat ttgctcttcc aactgttctc ggacaaagcg agcattttct
    23221 gcagaaacaa gattaatttt ggaaacatac gtaccacttt gaggtatcgt atacacgagt
    23281 tcttgctgac gtagttgaat caatgcttcg cgaatgggag tccggccgat agcaagatgt
    23341 tcctccagtg ccttttcaga tattttcctt ccaggttcta agtcagcata gataatttgt
    23401 ttacgaatcg cttgataagc ttgtgattgc aagctgcttt tcatctttat tcctccaaaa
    23461 tcatttctat gaacgtagta taacagaaga ggtctgaaaa acaatctggt atgtcacatt
    23521 aactagtaaa tttgtataaa tagtaaaatt ttggttgaca tagcgatttt tattgcataa
    23581 aattcacttg aatacaaaaa ctaatatgtt agtttaggag gggaaaaatg attgatttaa
    23641 acaactggag gaactttatc cctgaattat ctcaaaaaga agtagaaata cctcattttg
    23701 accgtgcatc attaagagaa aatggacaaa aaaatcctgt ctggatccat ttcggtggcg
    23761 gaaatcttta tcgaggattt catgcagaaa tcgctcaacg tttagcggat ctaggcgaat
    23821 tgtccactgg tgtcattgtt tgtgaaacat tcgatgaaga aataattgaa agggcttacc
    23881 aagattttga tgatttgatt cttgaagtgg tcatgccaag cagtggagag ttcgtcaaac
    23941 gtatattagc tgcgacagct gacagtctgt attgtcatcc agattgttct gaacactttg
    24001 ataagatgat tcgacacttt gaaaatccta gtttacaatt agttactttt gctattactg
    24061 aaaaaggtta tcgattgaaa gatacaaata ataaatatat aggaccgatt gcagtcgata
    24121 taaaaaatgg tccaaagaag ccatcacata caatgagtat tcttgctgtg ctactttata
    24181 aacgtttttt atctggagct tggccgattg cacttgtctc tacagataat ttttcacaaa
    24241 acggtagtca attacgtact tctttgctaa cgatcgcaaa agaatggcaa aaggaaggtt
    24301 atgtacaaaa tacatttatc caatacttat cgaatgaaga aattgttgcg tttccatgga
    24361 cgatgattga tcgtatcacc ccgaatcctt ccttgtcagt tagcaaaatg ttggcaaaag
    24421 agggcttttc aaatatgtcg attttccaaa ccgcaaaagg aaataatatt gctccatttg
    24481 tcaatacaga ggacatccat tatttaatca tggaagatca ttttcccaat ggacgtccga
    24541 aattcgaaaa agctggggta ttcttgactg atcgtgcaac ggtagataaa gcagatacga
    24601 tgaaggtgac gacttgcctt aatcctttgc atacgtcttt agctattatg ggttgtttac
    24661 tggggtatag gagcatagct gaggagatga aagatccaga tttatcgctg ctagtttctg
    24721 aaatcgggta caatgaaggc ctcccagttg tggagtctcc aggtatcatt gatccaaaga
    24781 tctttctgga agaagtaatt actgtgcgct tttctaatcc aaatattcct aatatgcctc
    24841 agcgaatcgc cacggatacc tctcaaaagg tggcgattcg ttatggtgag acgattaaaa
    24901 aatatatgga aatagaagtt aaagacccgc agacacttgt attcatacca ttgactctcg
    24961 ctgcttggct tcgatacctt attggcattg atgatcaagg aaaaccattc atcccgagtc
    25021 cagacccact attgacagac cttcagcata agctttccga tattcaactg ggtacgcaag
    25081 atacatcaca tattcatgag aaaatggctc ctattttgtc aaataaagaa atttttggtg
    25141 tagatcttta ccaagcagag cttggcagaa aaatagaaga aatgttcatc aagatggtag
    25201 caggaccaaa tgccgtacga aaaacattac aggattactt acaagaacat ggaggaaatt
    25261 tatcatggaa atgactttta gatggtacgg atcacaggaa gaaaagatta ccttagatga
    25321 aatcagacag attcctgcgg tcaaagggat tgtaggcaca ttgatggata taccagtagg
    25381 agaggtatgg cctagggaga ggatcaaaga actaaaagaa gaaattgagt ctcgtgggtt
    25441 aacactaaaa gtaattgaaa gcgtcaatgt gcatgatgcc attaaaattg gattaccttc
    25501 ccgtgagcag tatatcgaaa actataagca gacaatccga aacttggcag aatatggaat
    25561 agaagtcatc tgctataatt ttatgcctgt atttgactgg gtgaaatctg accttgacta
    25621 ccgcttagaa gacggctcat ccacgttagc ttttatatct gatgatattc cagatgatcc
    25681 aagagaaatc gtcgcgcgca tcgaacattc cagcaatgaa tttggattgc cgggatggga
    25741 acctgagcgc ttagctccta tcaaatcctt gcttgaagcc tatgcatcag tagacgaaga
    25801 aaaacttcga gaaaattttg cctattttct ccaatcaatc atcccaacat gtgaagaggt
    25861 aggggtcaaa atggctgtac atccagatga tccgccatac tcgatttttg gattagctcg
    25921 aatcgtaaag aatcgagaag atttagattg gttgtgtcgc atagtagaca gcccttataa
    25981 cggaatcact ctttgtacag ggagtattgc agaagatcca gaaaatgatg tatatgggat
    26041 tttagcagaa ttttgccaaa gagatcgaat cccatttgca catgttcgaa atattaaatt
    26101 tttagctaat cgagatttct atgaggcacc acataaatcc gaatacggtt cattagacat
    26161 gtaccgcata ctaaaaacaa tgtatgacaa cggatttaac ggatacatcc gtcctgatca
    26221 tggaagaatg atctggaatg agacaggaag accaggatac ggcttatatg atcgagcatt
    26281 aggtgctgtt tatctaaatg gattatggga agcaatcgaa aaaggaaaca aaagtaaaga
    26341 taattagtga aaaaaactgt aattttatta tgtaaataag aaggatgttc aaattcttga
    26401 tacataaatc cggaggttgt taataagcat tcggatttat gtatcttatt tggtctagga
    26461 agtaacttga ttttcttttt tcgattgatc catgagtgct tcttctttta attcaaagat
    26521 atattctgag atcctcataa tcactttttt ccagtcttct ggtgtgatga ttccttttaa
    26581 aatatagatt tttttgatat gagaaggcaa ttgtttcaaa gggcagtcgc tgataacgat
    26641 gttgcagttc tccataaaat cattttgtat tttgatcgat tcatttgaaa aaatttgttc
    26701 tatcttaacg atcagatctt tactgatata aaaatcacgc gtatagttca caccaatcac
    26761 aattcgcggc tgttttttgt agcgatcata caaatgtact aggtcttcaa ccatccattg
    26821 cacattttcc ttttctaaaa taactggtaa caagtgttga ttttgttctc tgaaatccat
    26881 ataaaattgt tcaatggcgg tttggacttc tttcaatacg ctatgttctt gataaagcat
    26941 gaaagtagga aggggcgtat ttggttgatt gaagttaaga aatccattat aaagtttatt
    27001 tcgtgcgaat gccaatgagt aaagaatctt ttctttttca ggtataaaca aatcaaatac
    27061 agtacaaagg gactccacca tttctcttga ataaagaaca tgaggaacgt ttgctttttt
    27121 taaaagtttg tattgctcca agcttgtttc ttccgattcg attttcgtga tagaaagtcg
    27181 agctaaaata ttcactaaga tgcgttgttc gcgacaaatt ggcagattag gtcgcaaaat
    27241 atctacagag agatcaataa aaaagtcaat atattcatac tctgtgaatt ctttttgtac
    27301 gtctttcaca gaagttgcag gaaaattctc tttacatatt tttaatagaa aatataattt
    27361 atctatcata ccgctattta gtcggtcaag tacttctttt ttaaaaaaag tattcatcat
    27421 aaaagccact acttgcggat cttcatcata aaattctgta gcagtacttg aaaagatgtt
    27481 ccaataaata tctaatgctg catattgaat ttgtacctct tttccagaaa aagactttct
    27541 gccattatta gaaaaggaga tagacaaacc gtactatgct aaaaatgcat taatcttttt
    27601 cattttcgta tacaaataag aggcactata gtttgtttgc tgactgaaac tgataatcga
    27661 atttgtctga cagctgaaca aagaagtcaa gatctgataa ctgactgatt cgttgcagta
    27721 tcttccaaat agacgataat agcagtaagc cggatcaatt ccttcaaaat tcaactttaa
    27781 aaaaggtttt tgcttatcaa tatgcagttc tgttagattg agacgctcaa agtcttgttc
    27841 taattctttg atgtatagta aaaaatttga ttcagacata tggaaatgat ctgctaaata
    27901 ttgtgtagta ataaagaaac gatgttcaga taggaaaaga aaaatatcta attttgtatt
    27961 gctttttttc ttcaagataa acgttttaat cgcttcttga gtttgcatga aagaggctcc
    28021 tttttactta aattgttaaa acttgaaaat attactgttt ttttcataaa tttagacaaa
    28081 aaaacagtaa acatataggt agaatctcta ctatcttatt tcttgcatac aaaaaaatca
    28141 aggagtgaga gagttatgag atattcaacc atcttaatat caacatttgc agccttaagc
    28201 ttatcgctta ctataaatca gacagtccac acagaggaga cagaaacagc caaatggaca
    28261 gaaaccaagt gggccaacgt caatcgaatt acctttcagg atgaattttc atccttcaat
    28321 tcaggagtcg caaaattaag aaaaggacaa acagcgacca tcaaagcaaa aattgactat
    28381 tcgggagact tagcacctat actacaatca gctgttacat ttgagaatgt tgatcccact
    28441 ttgtcgattg gctatgacga gaacactttg actatccaac acaagcaagc gatatttacc
    28501 ttcaccgtta cattaaatca ggacttgtta aaaccagcgc tttttactgt caaggtatca
    28561 gatggactac cgaacgatca tcatttaacg ccgtatagtc agcggcagac aattgagcaa
    28621 gattcagcca tcgactcagg aggagatctt gttgaagaac caacagataa accagaaaat
    28681 gaaaataagc cagaagtacc gcctacagaa aatccagacg gagaacagaa accggaaata
    28741 gaacctggag aagaacccga tacagaaact caaccagagc cagacaataa atcaaaacct
    28801 gaaataaccc ctggagaaaa accagatgta actccagaac cagatacgga ttctggaaac
    28861 caaacagtac cggaaacaaa tccagacaca gataatgaaa ctgaaaatcc agaaaaacca
    28921 gaggtagatc cagaagaaaa accagatgtg actccagaac cagatacgga cgcaagagac
    28981 caaggaatac cagaaaaaat aaacaaaaaa acaattcaag aggatggaaa aaaagagagt
    29041 aaaaaatcaa atctagcaat tctaaaaata aatgaagagc agctgaataa aaaatcacga
    29101 atatttgata gcgcccaatc agctgaaacc ttaaaaagta gcaaggatac aacctttgct
    29161 gtcctgaaac gaaaaataaa cagctaccaa aatccggaga gtcacaaaac aaagttatac
    29221 tgtggagcgg aatcattcta ttaagtatag caacaatgct ttctgcaaaa agatttaaac
    29281 aaaacagatc tctctaacat ttgaataatg tctgtgaacg catttttcaa ataaaaagaa
    29341 atatccagaa aatgacccca aaataccaga ttgaaatctg atatttgggg tcgttcatac
    29401 tttatctttc ttcatattta ttttaaggga tatctgtttc ttttggaaga tggttcggga
    29461 tctttttcac tttcaataca tctaaaaaga aggtaaagat cggaatgccg acaatcaagc
    29521 cccaaacgcc aaagaaccgt tcgctaacaa gtaagaccac aaatgtataa aaaatcggca
    29581 gttctgtttt gctggacatc aacttcggat tcagcacata agactcaatt agatggacaa
    29641 tcaaaatcgt aatcaggata taaacaacat ctcggatgcc tccttgggaa taagctatga
    29701 aagatagagg aatacaagaa atgataacac ctgctactgg gatcagactt aaaatgaaaa
    29761 tcatgatcgc taaactcagc agctgatgaa agccaaatac agccaaggcg attgtagtga
    29821 gcaacgtatt taaaagagcg acaacaaatt gagcttctaa taccaaacca aatgtattga
    29881 caaacttatc ggcaaaatag taaatatctt gaaagaacca agaaaattca ctttttaaaa
    29941 acaatcttga gaatacaatt gtttttttct tttcaatcat aaaaaagaaa cttaagatga
    30001 atgacatagc aaacgccact gccatttttc ctatatcctg taaatagcca agcgcaatcg
    30061 aagcgccatt ttgaagctgt tcaaaaaaat cgtttcgctc aatataagta tggatatatt
    30121 gtaaaacagg atctgcatct tttggctgat gctcgtaaaa attaataacg gactggatca
    30181 tgtcaaatgt ttgatgaata agcatcggga tatactttgt gaatgccaga tagataaggt
    30241 aaacgatcag tgtataaatc atgatagcta aaacacctgt agggattcgg acaaatcgct
    30301 gaatgatttg aacaagatga atcatcaaat aagtaaagat aaatgtgagc aaaatggttg
    30361 taatcatact tctaactaag aaaagaacaa aaacgacaaa taataaaaca gtgagacggc
    30421 gtattttttc attcgccaaa aaacgctgat aaacggtcaa atcgacaacc tccaaataac
    30481 aaaaaataaa cacgttattc attatatgat ggaatatgaa aataagaaag agcaaaaaga
    30541 aaaaccgcta aatgacgtta tcttatgagt tggttttcct cataaaatcg ttcatctagc
    30601 agtttcagcc ctttttctgg tcttgatgaa aattatctag catttttcgg gtcagatgtt
    30661 gcagtgtatc caattcttcc tgttcaagtg gaagataaga cttgatcttt tgtggaatac
    30721 cttctgcttg tttttgcaac gcagcacctt gagcagtcag cgtaacattt acgattcttt
    30781 catcacccgt ttcacgctga cgattaatat aatttttttc tgctaacttt tttagtaagg
    30841 gagtcagcgt accagaatcc agacacaaat catttcctag ctctttcact gaagtctttt
    30901 tcttttccca taaaactaac atcacaagat actgggtata ggtaagattc agcggcttga
    30961 ggaggggagt gtagtgtcta atgatttctt tagaggcggc atataaagga aaacatactt
    31021 gattaactaa atatagatct tccatttttc cacctccgct gttttactat agtgtatcat
    31081 agcggattta cacaaaaaag tctattcact aactgctttc agagtaactt ctatatttcc
    31141 agctgtagct tttgaatacg gacacacttt atgcgctgcc tctaataatt cttgcgtttc
    31201 ttccagagaa agaccttcga tagatccttc aatctctaca cctaagacaa agctcgtttc
    31261 actttctgaa taaagggaaa cttgtgcact gacagtagaa gccccagaga tatttttttg
    31321 ctgtttgatc aattctaatg cactgttaaa acatgcactg aaaccagctg caaacaattg
    31381 ttcagggttg gttgcatttt cgacgcgttt accaggagct gcaatattca attcgaatgt
    31441 acgatcggga ttatgcactt ctccagagcg tccgcctgtg ttgatcattt tcgtagaata
    31501 catttttttc ataaaattac cacctttaat tatattttat acaattgaat tttacacaat
    31561 ataattgtgg aaagcaaaga aatggattgg cataagataa ataagagaaa gcacatgaaa
    31621 cagacgcgct ttctcgaata gcaaacatat acaaaaaata tcagagacaa aaaataatct
    31681 catcataaac attacatata tttctcaaaa agttgttttc tttcttcaaa aattacttca
    31741 cgatggacct gatgaagcat ccataaataa ccaaaaggat ctctgaacat tgagttagtc
    31801 acaccaaagt ccatcatttc tgtcatttct tgaatctcta cacagccatt tcgtaatgct
    31861 ttattatgtg tgtcctcgat atcagagacg atgatattga accagatcgt atttggatgt
    31921 tcagcagtag gagcagtcaa accaagagac gtgttttcat ccagcatatg gaatctcgta
    31981 ccatacaagg taaaaatgac ttcattattg cctctagggt aatctgttgc ctcaatcagt
    32041 tgtatttcat caaagattgt ttgatacagt tctagtgctt tcaaactatc atttaataca
    32101 aaatcgattt ccgttccttt atgcataaag atctcctctc cttttcagtc gaacagttac
    32161 tagattcttt tattatacca gctaaagaaa acgcattcaa aaaaatagcg gatttactta
    32221 ccaactcatt cgattggatg ataagtaaat ccgcgtccca ttacttctgc tacgctgctg
    32281 acaatgacaa aggcacttgg atcgatttct tctatcaatg gtttcattcg ggaaaattca
    32341 tgatcagtca caacgatcaa taaaacctct tttgactttc gattataccc gccttttgcc
    32401 tctagcaggg taaggccacg tccaatctca gcacttaagc gaaggcggat gtcttccaaa
    32461 taatgttcac tcatgaccat aacagatttc tttcgattga tacctgtttc gatataagtc
    32521 atgacaattg aagtgattgc aatagataaa atagcataaa gaaactcttc tattccaaaa
    32581 ataaacagat taagagaaac aacgaatgcg tctgtaaaaa ataagccaat cgaagtattc
    32641 aagtggaaat attttttaaa aatgagggga gggatcgtcg taccgccgct agaagagttg
    32701 tttttatata aaatcgcaac tcctaaagca aaaatcgcac ttccaaaaat gacagacaac
    32761 aaccgatctg aagtaatcat tgtttctgga actattgcaa tgattaaagg aaaaaccaag
    32821 cttccgaaaa gtactttaaa aaagggtttt ttacctaaaa atagaagagc tagtaccaac
    32881 ataacgatat tcaacactaa aataactata gctcggtcaa agcctaaagc agcttccagt
    32941 aatattccta gaccgctgac acctccagca gcaatatgat gaggccctaa aaacatattg
    33001 atagcgactc ctaatatgaa gagggcaaaa ataatcttaa tgtatgtcca tataaattgt
    33061 ctcgtttctt tcatttattc acttccttaa ttcattaata atactcttat cttattataa
    33121 cttacttttc accttaagtt aactttaatt aataaaaaaa ttaggctagt ctaaattatt
    33181 atgtttcatt cttctagaat atacttctac gccaaaaact gttaaaaaac ttttacttta
    33241 gaaaattgga gaaattgatc gcttaagata aattcaggac actaaatagt acaggaagta
    33301 gactaaaact tttaaaagtt ttaagtctat ctatttggca agtttaaaaa ataaaaattc
    33361 ggtatcaaaa aactgttacc gataacattt ttgaaagcgt tgtcttttgc ttctctatca
    33421 cttatagtga tgacgtcata ttaaaacaca aggaggaaat caatggatac agctgcaatt
    33481 tatcatcgac cggaaagtga atatgcattt ctatatacga atacaagatt tcgtattcgg
    33541 cttcgtacta ggaaaaatga tatcaaaaaa gtatatgtgc tatgtggaga tccatatacc
    33601 ataacaactg aaaaatggta tcaaaaacaa cgaccaatga aaaaatgtct aagtaccaat
    33661 gttcatgatt attgggaaat tgaagtgacg agcgaaacaa gtcgcttaca gtatgcgttc
    33721 catgttgttg gggaagataa cacggactgt ttttacggtg atcaaggaat ttttccttat
    33781 cgagaaaatg tgattactga accaaatttt tattttcgaa taccttattt tcatcaaatc
    33841 gatcgattca ctattccaga atgggtaaaa gaaacagtat ggtatcaaat ttttcccgaa
    33901 agatttgcaa acggggacaa agcaaatgac cccgaacaca ctttaccttg gggaagtaaa
    33961 gatccagacc gagaagactt tttcggggga gatttgcaag gtgtgatgga tcatttggat
    34021 tacttgacgg accttggcat caatggcatc tatttttgtc ccatatttaa agccacatcc
    34081 aatcataaat acgatacgat tgattattat gaggtggatc ccgcttttgg cgataaaaaa
    34141 cttttaaaaa acttgattga tgaagcacat aaaagaggga tacgtatcat gttcgatgct
    34201 gtattcaatc atatgggtgt ccattcacca cagtggcaag acgttctcaa aaacggagaa
    34261 aaatcgatct ataaagactg gttccacatc cgttttttcc cagtagattc ttatcagatg
    34321 actgacttac cagaaactgc tgaaaatatc ccatatgata cttttgcttt tactccattc
    34381 atgccgaaac taaatacagc aaatccagaa gtgcaaaaat atttgctgga tatcgcaact
    34441 tattggataa aagaattcga tattgatgga tggcgtcttg atgttgctaa tgaagtcgat
    34501 catcattttt ggaaaaaatt cagagaagca gttacagaaa tcaaaccaga catctatatc
    34561 cttggagaga tttggcattc ctctcaagct tggctgcaag gggatgaatt ccatgcagtg
    34621 atgaattatg catttacaga ttcgattaaa gattattttg caaaaaagaa aatcacagct
    34681 tcacaaatgg tcagtggaat gaatcatcag caaatgcttt acagagatca ggtgaacgaa
    34741 ggaacattca acttattaga ttctcatgat actgccagga tcttgaccct ttgccaaggt
    34801 aacaaagaat tgatgaagtc tgtgatggcc tttatgtttc ttcaaaaagg gtctccttgt
    34861 atctattacg ggacagaaat agggatgact ggtgaagatg acccggattg ccgcaaatgt
    34921 atgatatggg agaaggagga acaagatcta gagctattcg gttttataaa agaacttgtt
    34981 tctctaagga aacagttgtc taagattatt tctgaaggtt ctactcagtg gctaatagta
    35041 aatgatcgtg aagataagct ctatttcaca cggaaactag agggacagat catctatgtt
    35101 tactttaatc agtcaaaaga accttgggta gtagaacagg aaaatgaggt gattcttagt
    35161 caaaattgtc agttgttaga agacgggaaa gcagagatac gacaatacgg atttttgatc
    35221 atgtgttgaa aactgttacc ggtaacaaca agaaagcgtt tgcaaatgaa gatttgtgcc
    35281 cattataata agggtgtaac agcacgttga atacaaagga ggaaatgaag atgaaaaaaa
    35341 gaagtgtgaa acagttgggt ttagggttaa tctctttagg aatcttaggc ggattagcgg
    35401 cttgtggcaa tggtaactct tcagcagaag gggaagccga tgaaggaaaa acgttaacag
    35461 tttccgttga ttctggatat aaagattata taaacgaaat aaaaaacact ttcgaaaaag
    35521 aaaacgatgt gaaaatcaag ctagtagaaa aggacatgtt cgatcagttg gaatcattag
    35581 cgttagatgg gcctgctgga aagggacctg acgttatgat ggcagcatat gaccggatcg
    35641 gtgcgttagg acagcaagga catctggcgg aagtaaaact gggaaatgaa gcagcttacg
    35701 atgaaacaga caaagcccaa gtgacttatg atggaaaaat ctacggagaa cctgcagtaa
    35761 tcgagaccct tgttttgtac tataacaaag atttagtcga tacagcacca tcaacgttta
    35821 aagatttgga gaatctatcc aaagacagtc gttttgcttt tgagtcagaa gcaggaaaaa
    35881 atacaggatt tttagctaaa tggactgact tttattattc ctatggttta attgcaggtt
    35941 acggtggcta tgtatttggt gatgatggca caaatccttc agatatcggt ttgaataatg
    36001 caggtgctgt agaaggaatc tcttatgcaa cagattggtt caaaaatgtt tggccaaagg
    36061 gaatgcaaga cataaaaagt gcaggagact tcgccagcca gcaattcatg tccaataaaa
    36121 ctgcagcaat cattgacggt ccatggcaag cgcaaacata caaagaaaac aatatcaatt
    36181 atggtgtagc caaaattcct actctaaata atggacagcc ttatcaaccg tttggcggag
    36241 gaaaaggatg ggtagtcagc aattatgcta aaaacaaaga cctctctcaa aaatggcttg
    36301 attatgtaac gaatcaggaa aatcaagaaa aattctttga aatggtaaat gaaatccctg
    36361 caaaccaaca agctagggaa acagcaaagg ctaaaaacga tgaattaaca acagctgtga
    36421 ttgaacaata tgaaacagct caagcgatgc caaacatccc agaaatgggc gaagtttgga
    36481 caggtgcaga aaacttgatg ttcgatgcag catcaggttc aaaaacacca aaagaatccg
    36541 cagatgaagc agtaaaaaca atcagtgaag ccatagaaca aaaatactga ttaaaatgat
    36601 aaccgtccat ttttataaaa aatggaacag gtgcttcaga taaaagtggc aagagcgaga
    36661 tggaaaatta tcttgctctt gttgccatta tcttaaaagc agtttgcggt ttagcaagtt
    36721 tagagggggc attcagatgc agcaagataa aacaagcaat atcaacgaaa aaaaaatcac
    36781 gctttgttca cttattccag ggatggggca gtttgtaaat ggccaagctt ttaaaggtat
    36841 cgtttttttc gccatattcg tcctattcat tggtcagatg cttctgggcg gatttcatgc
    36901 attggtagga ttggttactt tgggaagtat accaatggaa gatcattcat tatttatttt
    36961 gattgaaggc actttgcaat tcatcattac aatcattttc ttgatttttt acggtttgaa
    37021 tcttttagac gcttatcgag tagcgaagat gcgtcaagag ggcaaaaaaa tcaatcggac
    37081 gatgaaggaa gtcatacata atataggtga tgaaggtttt ccttatctat tgacacttcc
    37141 cgcttatctt ctcatgattt ttacgatcat tttcccagtc ctagttacat tgttcactgc
    37201 atttactaat tatgacttca atcatattcc accagccagt ttaattgatt gggtaggggc
    37261 taaaaacttt tttagtatct tctttttaag ctcatatcgt aatacttttt ttgcagtctt
    37321 ttcgtggacc gtgatttgga ctttatgtgc aacaactttg caaatcaccc ttggtattct
    37381 cacagcggtt gttgctcacc agaaatttat taaattcaaa cgtattttcg gtatcatttt
    37441 cttattacca tgggcggtcc cagcttttat tactatcatg agtttttcga atatattcaa
    37501 cgatagcgta ggtgccatca atactcaagt gattcctttc ataaaccagc ttccttttgt
    37561 agatatcagt gcaatctctt ggaaaacgga cccgaactgg acgaaagtag caattatctt
    37621 agtgcagggc tggttaggat ttccctatat ctatgtgatg gttacaggaa tcttacaatc
    37681 gatttcagaa gacttatatg aagcagcaaa aatcgacgga gcaaatgcat ggcagcgctt
    37741 ttcaaacatc acgcttcctg ctgtcttcct agtggcagcg ccaacattta ttacacagta
    37801 tacggggaat ttcaataatt tctccatgat ctatctgttt aacaatggcg gtcccggaag
    37861 cgtaggcgga ggagctggtt ctactgatat tcttatttcc tggatctata aacttacgac
    37921 tggaacttct ccgcagtatt ctatggcttc agcaatcacg ttgattatct ccgtcatcgt
    37981 tatctgtgtg tcattgctgg tatttaaaaa gacaaatgcg ttcaatatgg gggattaaca
    38041 atgaaaaaat ttcattcgag tgctcgattc caaaaacgac tttctcactt ttttacttac
    38101 ttttttcttt taatactgtc agtcattatc atctatccgc ttttgatcac agcaagtact
    38161 gccttcaagt caggaaatat tgcagctttt agtttagatc tttcaagtga ctggacttta
    38221 ggaaacttta ctcggctatt cagtgaaaca ctttacggga cttggtataa aaatacattg
    38281 atcatttctg tcctaacaat gatcgtacaa gtgactgtag taactttggc gggctatact
    38341 tatagtcgct atcgttttat cggccgcaaa tcaagtttga tgttcttttt agtgattcag
    38401 atggtaccga ctatggcagc attgactgcc ttttatgtca tggctttctt attagaagcg
    38461 ctcgataagt actggttctt gacaatgatc tatataggcg gtggaattcc aatgaatact
    38521 tggctgatga aaggatactt tgacactgta ccaatagatt tagatgaatc tgctaaactt
    38581 gatggggcag ggcatttcag gatttttttc cagattgtat taccgcttgt caaaccgatg
    38641 ctcgccgttc aagcattatg ggcttttatg ggaccttttg gggatttcat gttagctaag
    38701 ttcctgcttc gaacacctga aaatcagact gttgctgtcg gcttacaaac atttatttct
    38761 gatgtgacaa accaaagagt ctctcttttt gctgctgggg caattttgat tgcactaccg
    38821 atttgtctat tattcttctt tttacaaaag aactttgtta ccggcctgac atctggcgga
    38881 acaaaaggat aggagcagag tatcatgcaa acatttccgt taaactattt ttccgcatta
    38941 cgtacgccaa ctcaagtgtt tgcgggacgc aaactattat catggcctaa atttttcttg
    39001 atatttgtat ttttagtttc cttgatggtc atgcctgtga ctctttttta tgccaatcag
    39061 atccaagcga ttccattaga acaatttcta tccgttcatt cgctgatcga tgaacaagga
    39121 acccaaaaat tttctgaact tgaattatct gaaactgggt tacaagcatc gcaacaaacg
    39181 atagctgtga cccctgaaat tttggtaggt gtgtctttat ctgagaaaca acaatccgat
    39241 catggaactt ttattgattt tgagaaggaa caatgggtga tccaacaaaa ggataaatct
    39301 ggtatacgcc gctatacgat gaattatagc ccatctttcc aaccagattc tgtacgtacg
    39361 cccgaagatt tccaacgatt tttagaaaga gaattttatg ccagtaatcg tccaacaatc
    39421 attttatctt atagtttaag tcttggtctt gtcctatttg tcatgacgag tctcatttta
    39481 ttcggggctt cctttttctt gtggatgaca agaaaaagcc aactttcatc cattcataca
    39541 ttcaaagaga gtgcaaattt gatgctgaat gttatgggaa taggttccat ggcagcagca
    39601 gttgtagggt ttatccactt tgactttatc ttgatgctaa gtgtccaaac aacgataact
    39661 gtcttgctgc ttttatgggt ttttgtcaaa acaagattca aagataagcg tgtcgaatga
    39721 caagtttaag cgatttatgc gacaatgaat gtgaaaaaaa tgaaaaataa agtagttttt
    39781 gaaaaatttc tgaaaaaatt aaacttctta gttgattctt ttctttttta gtattaacgt
    39841 aatagggtag tttttttaag gaggaaaatc atgttagaga aaactttata caatcaatta
    39901 ttttcacgtt ctttctcgct ccctgtcgaa gtaaattatt gggacggtac aacgaaacaa
    39961 tacggagata cggacactcc tacccaaatc aaaatcaata tccatgaaga aatcccaatg
    40021 aaagagatta caagtaacgc atctttggca ctaggagaag cctatatgga tcatcgaatc
    40081 gagatcgagg gaagtatcca agcattgatc aacgatgctt atcagcaagc aaacagcttt
    40141 ttacgaggaa aagattatct aaaatggctg ccaaagaaag aaaaacatac gaaacaaaaa
    40201 aacaaagaag atatccatgc gcattatgac ttaggtaacg acttttataa actttggcta
    40261 gatccaactt tgacttattc ttgtgcttat ttcaatacac ctgaagatac gttggaacaa
    40321 gcacaaatca ataaagtcca tcatatctta gacaaactct ttatcaaaga aggcgaaacg
    40381 ttgcttgata tcggttgtgg ctggggaaca ttgatgttca ctgctaccaa agagtataat
    40441 gtcaaggcaa ctgggatcac actaagtgaa gaacaatacg atttgattac caaaagaata
    40501 aaagaagaac atttagaaga taaatgccgt gtattattaa tggattaccg cgaactaaaa
    40561 ggcgaaacgt ttgatcatat cacaagtgta ggtatgttcg aacatgtagg ttctgaaaat
    40621 cttgaaggtt atttcaaagt ggtaaaagat ttattgaaac caaaaggaac agctttgatc
    40681 catggaatca gccgacaaca aggcggtgcg acaaatgctt ggatcaataa atatattttc
    40741 cctggcggtt atgtccctgg tgttactgaa ttagttgata atattacaaa aaacgatttg
    40801 caactgattg atttggaaag cttacgtcgt gactaccaat tgacattaga acattggaca
    40861 aagaattttc atcaagtcca agatcaagtc acgaaagaaa tgggtgattc cttctatcga
    40921 atgtgggatc tatatttaca agcttgtgca gcttcatttg aatcaagtaa tatcgatgtg
    40981 attcaatatt tgcttgttca acctggaaac aatgacatcc caatgcatcg tgcataattg
    41041 aggcaaaagt actgtttaaa gactatttaa aaagaagcta agttttatga tgctccagaa
    41101 gtgaactttt ttgtctaact tctaggattg tctctaaagc ttagtttctt tttctttata
    41161 ttttcataat aaaagtttgg ttctttaatt tttaaacatg acagaaaatt ttttcttcgt
    41221 tatacttgta atagaatact attttataac aaaggggatc gaatcaatga aaaaaactgt
    41281 tatttatact agtttatttg cagctttgtt tttagctgct tgtcaatcaa ataccaatca
    41341 aggcacatct gggaatacgt cttccagtag cgaacagcgc tcttcacagg tatcggactc
    41401 ctcaacaaaa gcaagcacat cttcgtctag ccagacaaaa agttctagtg aagcaagtac
    41461 tcagtcagat atcaaagtgt ctgcagctcg tgcgatcgaa ttgttccaag ataaataccc
    41521 agatactgca attactagtt tggaattaga tagtgattgg ggatcttact tctacaagat
    41581 agaaggtgtc gatgatcaaa aagaatacga agcaagaatt gatgctcaaa atgaaaaaat
    41641 ggagacagag aatccagaaa ctctagatcg agatgaacaa aatggtcaaa agaaaaaaga
    41701 agatggtctg gatgttgata aattgatcac gattgaggaa gcagcaaaaa tcgccgtcga
    41761 caaagtagga agcggtacgg caacagattg ggatttggat aaagatttag gtactactta
    41821 ttgggaagtc aaagtaaaag acggcaatca aagtacatca gtcaagatca atagccaaac
    41881 aggcgaagta ctgtcaactg aacaagatga ttaagaattt cttcggaaaa caagagttga
    41941 tttttctttt agtcatgata cactagacaa aataacttgt ttgaaggagc gatttacttg
    42001 agtttagaaa cgacctttgc caatcatatc tttgccaatc ctttgatgaa tgcatctggt
    42061 gtccactgca tgactaccca ggaattagat gaactggctc attcagaagc aggagcattt
    42121 atcactaaga gctgtacgat caatgaacga aaaggaaatc ccgaaccacg ctattttgat
    42181 gttcctttag ggagtatcaa ttctatggga cttccaaact taggtttttc ttattatcta
    42241 gagtatgctc ttgcgtatga aaaagtgcag gaaaaccaaa atcaaccgtt atttttttcc
    42301 attgccggga tgagtgtcca ggaaaacttg gaaatgcttg agaaaataga aaaaagtggg
    42361 tttaatggga tcactgaatt aaatttatct tgtcccaatg tgcctggaaa accacaacta
    42421 gcttacgatt ttgaggccac ttatgaaaca ctgaaagaag tattcagcat cttttctaag
    42481 ccgctaggaa tcaaattgcc tccttatttt gattttgctc attttgatca gatggctgat
    42541 atcttgaatc agtttccatt gacctatgtc aatgctatca acagtgtggg caatggtttg
    42601 tatatcgata cagaacaaga agcagtagtc atcaaaccaa aagaagggtt cggcggcatt
    42661 ggcggagagt atatcaagcc gactgctttg gcgaatgttc gcgcttttta tacacgtttg
    42721 aaaccagaaa tccaaatcat tggtacaggt gggattcgga caggacaaga tgcatttgaa
    42781 catctgctat gtggcgcaag catgttacag attggtaccg agcttcataa agaagggccg
    42841 gaaatttttt ctagaatcat aaaagaatta acgcagatta tgtcagaaaa aggatatacg
    42901 tcaattgacg aatttaaagg caaattacgc accatttcat agcaaagaga cgaaggtcat
    42961 gattgatctt cgtctttttt tataaaaaac actcgtctgt tcattcctgt gcaagcgtac
    43021 tcattgtatg ttagacgaac ttgtgggaaa ataacagagg caaaaaggag gataatatga
    43081 atgatgcaac agaattagca aaagagctta aagaaaaaga atttagtttt caagaactag
    43141 ttacggatat acagaaaaaa ataaagaagc aaaatcatga attgaatgcg tttgtcgcgc
    43201 ttgaaccatt cgagagtaat tcccacgagc tattgattcc ccagtcagaa aaagggccat
    43261 tggctggtat cccatttcct ttaaaaatgc tcggtcaaga aaaaaaggga tggctagcta
    43321 cttcaggatc tcgtttattt gaaacacacc gagcttctca tacatctaat tatgtgcggc
    43381 aagcagaagc gataggtcta gttccttttg gacaaaccaa tgcgccagag tttggtttca
    43441 aaaatattac agacccagtc atctatggcc cagcaagaaa cccttggaac cttgaatgta
    43501 caccgggcgg atcaagtgga ggagctgctg ctgcagtttc atcaggaatc gttcctttag
    43561 ctggtgctag tgatggcggt ggttctatta gaatacccgc ttcgttttgc ggattgattg
    43621 ggctgaaacc ttcgcgaggg acgatgccag ttggtccata tgcttggaga ggatggcaag
    43681 gagctgctat tgatttcggc ttgacagttt ctatgagaga cacggaggct ttattttatg
    43741 ggatgagaag tatccattcc ggtgcgccat atcaagtatc gccagttgag tggcaaacac
    43801 atccaagaaa aaaacggttg aagatagctt tttgtgttga ttcaccgatc cactcacaga
    43861 ttagtgaaga ggcagtactt gctgtaaaaa aagctgtaga tttccttgga aaagaagggc
    43921 atgatttgac agagattcct tacccactag atggtcgtaa actgatcacg tcttattatc
    43981 agatgaatgg cgcagaaaca gcagcaatga tccgctcgat tgaaacaggc atccagcgag
    44041 ctgtcagaaa agaagaactt gaacccattt cttggacttt gttccaatat ggtcagaagc
    44101 tctctgccgc tacttacatc gattctctgc atgtatggga tgaagcagca attgtcatgg
    44161 aaaacctaca tcaagaatac gatctattcc tttcaccaac gactgcattt accgccccta
    44221 aaatcagcga agatttgcaa agtgatgcga taagagcaca gatggaacac atcagtgatt
    44281 gttctgaagt agaaggttta gacgttgttt cttccatgtt tgaggagagc ttgaagatca
    44341 caccttatac acagttggct aatctgactg gacaaccagc aatcagcttg cctactcatt
    44401 tgacaaaaga aaacatgcct ctaggaatcc aatttatggc tgccagagga agagaagatt
    44461 tgctatttca aatcggctat ctatttgaag caaatcatca gttccattta ccaaatccat
    44521 ggaaatagca gaaacaagga gaagaagaat gaagaaaaaa agattgtttg ctctaataac
    44581 tagtgccttt tttattttaa gcgcttgcgg tacagctgcc gctaatcagg aagataacag
    44641 ctggagtaat gtagaaaaaa agaaagaatt agtagtagcg acttctggca cactgtttcc
    44701 cgcatcttat tataatgatc aaaatgaatt agttggttat gatgtagatt tggcaaaaga
    44761 ggtggctaaa cggttaaatg tcaaaatctc ctttaaagaa tacaacgtgg atggacaaat
    44821 cacttctgtt tcaaaaagag aatcagattt tgctgcgaac gattttggta tcacaaaaga
    44881 aagagaaaaa aaattctccc tttcagagac tttcaaatat tcatttgaca gtatgatcgt
    44941 acgaaaaaaa gatgattcag gaatccactc acttgaagat cttaaaggga aaaaagcagc
    45001 tggcgaaccc aatactagct atatgcgttt agcggaaaaa cacggagcta aattagtcac
    45061 atatgacaat gccacgaatg atcagtattt aagcgatgta gcaaatggtc gaacagatgt
    45121 tattctaaat gattattatc tgcagaaaat ggccgtatct gctttaccag atatccctgt
    45181 aaaaatactg gagaacgttt atttcaatga aaacgaaagt ggttttttat tgaaagaagg
    45241 aaatgatgct ctgaaaaaga aaatcgatgg cgtattaaaa gagatgaaag aagatgggac
    45301 gacaaagaaa atatctgaaa aatattttca aactgatgtt tcagtcaaac cgaaagaaac
    45361 gatggctgag tcggtgagtg agtgatgtac tttcctctaa ttgatttttc cctcatgtgg
    45421 acttcacttc ctttcgtact gcaaggcttg gtatacacat tagggattgg cttcgtgtcg
    45481 tttcttttag gaaatctggt agggttgctt ttgacagttt taggtctttt ggattggctg
    45541 cctctgaatg tatttattcg tttttattta tcgttcttta gaggcattcc cgcactagtc
    45601 ctgctgtttc tgttatattt tgggttacct tatcaattaa gtgccttaac ggcttcagtc
    45661 atctgcttta cgattaccag cagtgcattt attggtgaaa tctatcgagg atcactagca
    45721 ggcgtttctt caggtcagtg ggacgctggt ttagcttctg gtttttcttt ctttggggta
    45781 ttgaaatata tcgttttgcc gcaagctttt cgaatctcag taccggcact cagtaacgta
    45841 gctatggatc tagtgaaagg tacttcgtta gcagcaacaa tcacagtgcc agaaatattt
    45901 caaaaagcaa agatcatagg cggtcggaca ttcgattatt tttctctcta tattttagtt
    45961 gcactgattt attgggtcat ttgtatcggg atcgaaaaag tgcaacagta tttagaaaaa
    46021 cgttctatgc atcagtcaat ctgaaaaata gaaatggtgc tagcgtggct ggcaccattt
    46081 ctattttttg tttcttgtat attctgccta gtattttaaa accatttcat gtaaaatgta
    46141 aatcgaaaat atgttcggag gtagaaaaca tgaagacccc gctaaccatc gaaatagaat
    46201 cctcgttata ccaatattgc atcgagcaag gcggcttggt tgtagaagaa gtcacgatgc
    46261 ctgatgacca aggaattgta gatacgctta gctgcttgac aaaatttgat ggaagccgag
    46321 aatggcgatg ctatgagctg aaagtgacaa aagcagattt tcgctctaca gccaaactgt
    46381 catttgttgg acattataat tattttgttt taccaagtaa actatatgaa gaagtaaaaa
    46441 ctgaaatccc tacaggaata ggtgttttag tgtatcgtcc gtacacggtt gaagaggaga
    46501 tgcctgcctc tggtacattt ctgattgcga aaaaagcagc aaagaaagag cttgctgtac
    46561 cggaagaagc tttgacaaac cgcttcatgg cttctttatt cagggaagta agaaaagcaa
    46621 agcaaatgga gcgaggcgta aaatattttc caacagatca attgtacaaa gaattgaaaa
    46681 gacgttcaga aaatcaagat ccattttcta acgaacgata ttatgaacgg tttttagagg
    46741 aaatagctag tcaggcaatc aatgagttgc aagaagaagt tatcgcgctt cagcatgatt
    46801 atgaatattt gaggcaacaa cagcagatcc gtcggttgcc tacagagcca ttggagtgaa
    46861 aaatatgtac tatccttatt taagaggaaa acaatttgac ctattagcat taaaagaagc
    46921 actgtcgcga ggattgctct ccaataaaat ccaaccagtg atcgaaccag tacgtgattc
    46981 cgctacgctt aaaaatgtga tcgaattatt tcaaaaaaag caccatcgaa tcgctgtgat
    47041 ccaaaatccc caagtaggac aattcaaatt attcgatcag catgtgcaca gttggacagt
    47101 caaagaaaat agctctgtcg tccctgcaca gatcttaacg ccagaaaatc tcagtgaagt
    47161 actggattca cctccagcgt ttcttgtttt cgatggacag cactacccaa aagatacaga
    47221 ggtgtggaaa cagttggcag gagttgattc gaaatttttg ataccagata ctagtcgctt
    47281 tcgcatctgg ctgcccgaaa acaaaattgt gatccgtgac tcttttcaaa cgagaaagca
    47341 tgttgaaagt tatgcagata aaacagatga cttttttagt gatgactatc tttttttcca
    47401 cgcagacgga tatattggat tttctgattt cacgatcgaa ggtagccgtt atttcgataa
    47461 agggttccct agccgagcgc ttgctatcca tctaacgtat attgatgctt acggcaatat
    47521 acgcgtgaaa cattttgtct ctgacagtaa cgactctgct aaagaccaag cattgaagtt
    47581 tcttgaagca ggagataaaa tgaaaaaatg gatcatgcgt caccatcatc aactcttgat
    47641 tacttcagga atgattgaat tattggctct ttaccagcaa caaaaattcc ctggactggg
    47701 agtgttgaaa aagtggtcac ttatgcatca tttggaactt attagtcacc tgctagatca
    47761 tccaaaagat tggcttaaat attattcaaa agttcaggaa ctatatgaag tgatccaatg
    47821 attagataat gtaaatgaaa aaggaataga aaagaggaaa ctggatgtat attcggcaaa
    47881 caaggaaaga agaagtagaa gaagtggtag aaatcattaa tgccgcagcg gatttcattg
    47941 cttcgcaagg ctctcctcaa tggcaaaacg ggcagaaacc aacaaaagaa acgattttga
    48001 acgatattga aagaaaagaa agctatgtat taatagtgga agatcaaatt gctggaacag
    48061 ctgcattagt ctcaagcgtg gatcccgttt acacaaaaat agatggtagt tggaaagaat
    48121 caactggtcc ttatttatcg attcatcgtg tcgctttagc agaaagtact cgaggaaaaa
    48181 aactagcaaa accgttctta gaacatttgt tgacgattgc agccagcaaa aatatccaag
    48241 atatccgtat cgatacttat ccagctaatg aaccgatgga aaagaccatc tattctgctg
    48301 gattcactta tcaagggatg attgaatttc cttttgaaca cggcgaaaga aaagcatatc
    48361 aaataatcat caattgacta taaaatcatc tgagcaaaga taaagacatt aacactactg
    48421 aaaaaaacaa tcatatgata gccaagaggt tgtaacagaa gtggccagct tcaaaaaaca
    48481 agaaagaatt tccgaaaatg cttctcaaat ttttgtggaa ttttagcgta ttttacgaaa
    48541 tagctgcttt ctgaaactcc cttcatcgag aggtcgtgac atagcttttg tcacgacctc
    48601 tcggcttatc ctacgagtat tattcgatac atttatatca ataatccttt ttttatttta
    48661 tctgcagctt cttggaacgc tccatcgttt gacacgacta aaagtgaggg gatagctttc
    48721 aaacgtttca actgtgctaa atcttctaca taaaccgcca gtctggctga aatctgttca
    48781 gatgaatctc cacgtttttt caggcgcgat tgcaatgttt ctttagagat cgtaataaag
    48841 ataggacgga ctaccgtccc gaaatactca tataaatgcc aaaaaccatt tgctgtcaaa
    48901 acggcgtaac aatttcctac tgtcagtttc tttttaattt ctgctttact ggaaccatat
    48961 ttatgttgag catattggtc ccactctgca aactcatttc ccttgatcat ctgattgaat
    49021 gtattgtcag aaacaaaata ataatcctct tgatcttttt ccccaggtct tggtgatcga
    49081 ttcgtgtaag taatgatctt ttgttccggc tgaaaaatag cttctgcgag tgaagttttt
    49141 ccagaaccag aaggaccgat aaatacatag caataattca ttgtcatttc tcctttgatg
    49201 attgaacaag tgaaaaagct ttggaaaata tttccaaagc ttttaaaagt aactttgctt
    49261 ttttgtatat tataccagca agccagtcac aatagcactt aaaaagctga ctaaagtcgc
    49321 tgttagcaaa attttcaatc cgtgttttgc gacaagattt cctttttctt catttaatcc
    49381 cttcatcgca ccagaaatga tcccgataga agagaagttg gcaaatgaaa cgaggaagac
    49441 agatataatt gctgttgtac gctcagtaaa atggaaatta ccatttgcta attctgtcat
    49501 agcaacaaat tcatttgtaa ccagtttagt cgccatgata ctaccggcat ccactgcttc
    49561 tttccatggg attccgctga tgaaggcaag aggggcaaag atgtaaccta ggatctcttg
    49621 gaaagtgatg ccgaatatcc cgtggaagat agcattgatc atggcaatca aagcaacaaa
    49681 accgataagc atagcagcaa ccgtgatcgc cacatggaaa ccatcaagaa tgtattctcc
    49741 caacatttgg aaaaatgtct gttctttgtt ttcttcaatg atcagctcat cttctttttc
    49801 ttctaataca tagggattta caattgaagc cagaatataa ccgccaaata agttcaaaac
    49861 aagggcagtg atcacatatt tagaatcgat cagtgccata taagatccga cgatcgacat
    49921 agaaactgta gacatagccg atacactcat ggtgaacaaa cgtttctcag ataggaaggg
    49981 taattccttt tttatggaaa taaaaacttc cgattgtcct aaaatagcag aagctactcc
    50041 attataagat tccaatttgc ccatcccgtt gattttactt aatcctagac caacagcttt
    50101 cataaataat ggaagaatct tgatatagcg tagaataccg ataatagcag aaataaatac
    50161 aattggcatc aatacggaaa gaaagaaagg tgcacttcct ttatttgcta caccggcaaa
    50221 aacaaagtca acaccttgcc cagcaaaagc aagaagatga tcaaacattt ttgccaatac
    50281 agaaacaacc gctgtaccaa aagttgtacg taataatacg aaaccaagaa caagctgtaa
    50341 aacgaacatc acaattagtg gtttcaattt gattgctttt ttgttactgc tgatcaaaaa
    50401 agcaagccct aatgtgacaa ataaaccaat aatacctaat aaataactca aataatcact
    50461 cctattccaa attccgtttt attttacctg ttttgcaaat aaaatactaa tctttagtga
    50521 aattttcatt acttttttag agtgaaacgc attcattttc cattagaaaa taaagcgaac
    50581 aaaataaaag gtttaaaaag tgtaacgtct tagtaaagtc tataaaaaaa cgaaactatc
    50641 gattaagcta atagtttcgt tttcaacatt tataagtaaa ctttttctat tggtaatata
    50701 gattttcgga agggtagaca ggtgcgcagg taatatcgat tcctagtttt ttcaatgttt
    50761 gttcatcatt tttattgacc atcacagtag agtgggcttg tgctccctca agttcagcta
    50821 gtttatcata agcaacttga gcgattggat tcgttactgc actgattgat aaagcgatca
    50881 agacttcatt gatgcttagt gcatccaaag aggaatgaag tgtctttgat ttcaatcctt
    50941 gtattgtttc aagaactaat ggtgacaata aaggaatgtc atctgcgata tgggctaaat
    51001 gttttactgc atttaagata actgccgctg acgcatccat caatgaactt gtacgcccgg
    51061 taatgatgac ttcatctggt aattcgattg caatcacagc agaaggatct tttgtaggac
    51121 tttgcgcttg aatcttatct gcatagtcat gggcagggac taccacgttt cggtcttcag
    51181 gacgtaaacc gacttcttcc ataatcagtt tgattcgatt aactgcttct tcgtcgatca
    51241 atcctttttt gaagtcgcat tctgtttgga aataacgacg gatgatttct tgtttagagg
    51301 cttcttggac aacggtatca tccacgatcc caaatcctac acgattcaca cccatgtcag
    51361 taggagattg ataaacagat tctttaccag taattttttc aataatacgt ttgattacag
    51421 gaaatgtctc aacatcccga ttatagttca cagctacttt attgtaggca tcaaagtgga
    51481 aggaatcgat catattgaca tcttttagat cgactgtggc tgcttcataa gcaatgttca
    51541 gcggatgttt caacggaaca ttccatactg ggaaagtttc gaacttggag taaccagctg
    51601 catgtccttt ttggctttca tgatacagtt ggttcaagga agtagcgagc tttccacttc
    51661 ctgggccagg agctgtcact acaacgattg gtttagttgt ttcaatatac tcattttttc
    51721 caaaaccatc ttctgagacg attttttcta cgttgactgg atagtcttcg atttctgagt
    51781 gtttatagac cttgatgttt cgtctttcta gtttattgat aaagactttc gtggcaggct
    51841 gtccactata gcgagtgata acgacactgt tgattaataa gccataacca cgtaattcat
    51901 cgattaaccg caagatatcc atatcataag taatcccata atcaccacgg atcttattac
    51961 gttcaatatc tccagcatag acgcaaatca gtatttcagc ttgatctttc aatttttgca
    52021 gcaatttgat tttcgcatct tcttcaaatc ctggaagtac acgtttcgca tgcttgtcgt
    52081 caactagctt cccaccaaat tctaaatata atttgtcata gtggttcact cgttccaaaa
    52141 tatacgctga ctgctcttca atatattttt ctgaatcaaa accaattttt ttcactaggt
    52201 tccctcctta agtactaaag cattttttca tttttttgag gattcgtcaa ctggaagagc
    52261 gaaattcctc aaaaaaacaa agaaaatggt ttaaaccaac ttgtctagat agttattcat
    52321 tggtatgata gtttttgata aggaaggtgc tacatgaaaa aaagacagaa aaagaaaaac
    52381 gcatataaac actatatccg ttcgattttt accggttatg aaagaatgct tgaagatccc
    52441 gaattggaac aattgacgtt tacttatcta aacgaagaaa cgcagctaac tcgtgatgag
    52501 catcagcgca ttcatttcac aacaagagat ctgcctagta aataaaaaat aagataaagg
    52561 tcatcggatt cgattctgtc ctatcagatg gaaagactag ttgatcgaaa aaaaggctgt
    52621 accaaatttt ggtgcagcct gttttatgga gcattgcccg ctctttttta taacaatttt
    52681 ttacagaaaa tggtactatt gaagtattaa taagtgaaaa acagatgagt aagaggaggc
    52741 aagtagtatg aaagagccag atacaaatgc aaattatgag ccagacgaac tagaggagac
    52801 gaatgcggat caggatacag gacaagaatc gtcttctttt tacgatatca aagaaaaagc
    52861 aagcatgaat ctttcccaat tattagaaga cttacaggct tttgaaaaag ctgtccgcga
    52921 agaagatatc cctgagatct accgtttata caatggccga ttgaatgaag aattaaaaag
    52981 aagctcaaat gaaaatcacg agatcgatca attgctgatg cgtaagatcc atgatcgttt
    53041 tttacaggaa tttcctttta tggaacaagt agaaaacatc tcgccaacga tgagctatta
    53101 caaaatcggc acttattacc atgatcggcc cacggtcggt attgatgcaa gtctgccaga
    53161 aatttttgtt ttgcctaaga tcgatgaaga gtgggagaaa tattcccaac cggagtcaac
    53221 ggactatgac aaaaaaatca atgagctaga cgcaaaggtg atcactgctc agacagaaat
    53281 tgaacgttta gatgaacaaa taaaagaaat caatcgacaa atgactgatt tgaacgacaa
    53341 taaaggattc ttgaatcgta aaaaaatcga ggaagaaatt caagaattag agaagaagaa
    53401 gcaagtacta tctaatgaaa aattaggctg gcttccttat attgagacgc ctgaaacaat
    53461 ccagcagcaa aaagaagcat tgaagcaaga agcaagagca gaccagcttc gtgcagccat
    53521 cgtagaaaaa gaacaaaggc aaatcagacg ctatttcggt agcaaagagg gattcggtca
    53581 agcaatccat gaattcttga tgaattatct tggcaatgag aatcctaata atcaatctaa
    53641 tgaaggaggt tccgagtatg aatgaacacg atgaagcaga atggacaaaa aatgaagaac
    53701 aaacagaaga tcatgaagaa acgaacactt cattggaaga acaaacaagt aatcctctgg
    53761 atgacacaga agaaatccct atgctagagg aaacaaaaat acacgaaaaa gaaaaagtaa
    53821 ctccgacgaa tacgaatact gcagaaaaca cccatgctta cgaaataact gaaaatcccg
    53881 ggtatttaga aggaaaagag caagatcatc aagatatcgt cagtttgatt gaaacagcgg
    53941 aagcagaatt agcggattta cgtatgagaa aagatttgat cgaatcagat tttccacaat
    54001 tattagaatc atatgatcgt tttatattga atgaagaacc aatctccacg atcggaaaga
    54061 caagcctgat cgaatacttc acgtatgaat tactgaaacc gttgaatgat attggtctgg
    54121 aacggtcaaa ttcagcttat gacacatgga aaacaagaca tttttcgatc aattatacgt
    54181 tgaatggaca aaacgaatta gtcttttcct ttgtattacc gaccatcgat caacgatacc
    54241 aagtggaatc gatgagtctc ctaaacgtat caccagagac aatggaaatc aatgtccaag
    54301 atgaccgggt gctttctttg atccgacact ggtcagtgga tcgagtattc tctgcagggc
    54361 agatcacgat attcaatcac aaattgaatc aattattagc ccatgcaaga acacttggtt
    54421 ttaccgttaa tcagacatta ctagacaata cgaagccact tcgtttgaat cttcagagcg
    54481 aatttgaatt gacgaatgaa gtattggatg atatttttat cgtggcaatg aaaaatccat
    54541 cctacgatat ggaaaagatc aacgaaacta cttatcaagt acgattagat aaaggacagt
    54601 ctttaacgat aacaacaaac gaaaaagatc aaacagtctt gtcgatctca tctggagact
    54661 acccacgatc tgtcattgat ttctttatca attacgaatt cttggtccca ttaatggtga
    54721 gaaagtcctg attggataag ttggattacc agatgtcatt cagataattc tgttccaaag
    54781 gacttgcggc agttatccag aataattgtc acaagtcttt tctgtatgta ctaaatggac
    54841 gtgaataact tgctgatgaa ggagggattt cattaggaat gtccacaaat aaaatagcac
    54901 agcttagctt attgtctgct gcttgtgtag caggtaggat cgcttttcaa tttattccga
    54961 atttccagcc aatgactgct atttttttat ttatagtact ttacttaagc ttaaaagatg
    55021 cgttaattgt catgagtctt tctattgcga tcagcagttt ttattttggt gttggaccat
    55081 gggtcatcgg ccaatggatc agctatactg ttgttctgag tttgttttca atcgtctgct
    55141 tgagaaaaac agtgcaaagg aatctatggt taaagggagt ctgttttttt ctggcgggca
    55201 tggtttacgg aataggtatg accgtttttg atacgctgtt atatcgtttg cctcaacctt
    55261 ggatctatta ccttcaaggg gtgtcgtttg atttgatgca cagtataggc aatgttgtgt
    55321 tctttcttgt ttttctgcca gtcgtcaaac gtttttacaa tcattcagga ggaaaaaatg
    55381 aaaaaaataa tttatagttt gcttgcaatg agtgtcagta tactcatttt gacaagttgc
    55441 agtacaacct atacacaatc ggcttccaac caaaaggagg aagtaaaaac atcgcaaaaa
    55501 gcaacaatca cgttacaaga aaatggtcaa aacttttcgg aaaaagaagt gttcttcaaa
    55561 aaaggcgatg atttactgag tattttgaaa cgaaattttg aagtcaaaga agataacggc
    55621 ttcatcactt cgcttgaagg gcactcacag gatgagaaaa atcaactcta ttggatgttt
    55681 actgtggacg ggaaaagtgc tactactggt gccaaagaga tcaaattgaa tgatggacaa
    55741 tcggtcattt tcaatttgtc taaactataa gtttcataaa aaactgttaa agggcgaata
    55801 ttgccataat tcagtgagtt cagttgatga aagaaggaaa ataacatgga aaattttaaa
    55861 aaaagcggaa tgttttccgt aattgctgct ctaggggcta atattctagt tgctatcagc
    55921 aaatttgtcg gctatgcaat cagtggcagt gccgccatgc taaacgaaag catccacagt
    55981 atcgttgatt gcagcaatca gattttgctg ttattcggtg ataaacaagc caataaagga
    56041 cagagcgagt tgcatcaatt tggtgaagga cgagcaaaat actttttcag tacaatcgtc
    56101 gcaatgatgc ttttcttcgg cggtggggca cttggggtga tggaagcttt tgaaaagttg
    56161 acgcatccat cccatgaagt gggtaatccg atcattgtta tagcaatact gttatttggt
    56221 ttggtaatcg aaggaagttc gttacgcgtg gcaatgaaag aaatcaaaga attgaatact
    56281 gaaaaacttt ccacgatgcg ctttttgcgc gaaagtcgac acagtgaaat actgattatt
    56341 tttacggaag acttgtgtgc tgtaatcggt ttggtcttgg ctttagcagg aacaatattg
    56401 acttatgcaa ccgggattgc tgcatttgat gcgatcagcg gattgctgat cggtttcttg
    56461 ctgatgggag ccgccatttt cctagcaaaa gaattttata gtctgatcat cggtgaaagc
    56521 gtgacagcaa gcgatttatc aaaaatcaag ttagcttttg aacgtcctga gatcagtcgg
    56581 ctgatcgata taaaaacagt tcatttaagt ccaacggaga ttttagttgc agcaaaagca
    56641 gatatcgttg gtaacaaaga gaatgaagct tactcggtca tcaatgatat tgaaaaaaat
    56701 atgcgtcagt ctcttcctga caagaagatg tatatataca ttgaaacaga taaatatgac
    56761 ccaaattatt ctcgtaaata aaacgtttat tcatgaattg aagcaaaaat gagaaaaata
    56821 taatcttgtt tttaaataaa acggctacaa tttagcgaat ttatgttaca atgagtccgc
    56881 tgtttatttg ttgtaattaa aggagatatt ttaatgaaca aaaagaaaag tgcgccatgg
    56941 cttcaagctt tgcatgcagt gatgccactt tgtataagct atataccagt aggattagct
    57001 tgcggagtat tgttgcaaaa agttggattt gatcctctct tatcaggact gttatcgatt
    57061 ctggtctttt ccggtggcgc gcagttttta gttgcatcta tgcttacgac gcaagcatca
    57121 tttgcaacga cattattgat ggttttcttt ttagaattac gctatacatt attaggagca
    57181 agtcttgccg gttttatgaa aaaagaaaag cggacgtttt tggctgtctt ttctcaatca
    57241 ctgaatgatg aaaactatgc ggtgaactac ttaaagtttt caactgaccc ttcctggaat
    57301 aaaagaaagg ctttatatgt caattggttc tcaatgactg catgggccgg tagcaatatg
    57361 atcggtaatt ttttcggctc ggtgattcga gtagatgctg accttgtcca ttttgctttg
    57421 acggccatgt tcattttcat gtttatcatg cagatgaaaa gcgcactact gatctttaca
    57481 ggtttattct caggcgtatt aggggtcgta ttcatggtat tattccaaaa tactttcgga
    57541 ttgattgcag caacagtcat tgcttcattt gccggattta tgctggagaa ggcattcaaa
    57601 aaagaaaaag ctaagaaact aagagaccgc ggtaaagatg tcaatgagcc gctcttcaac
    57661 ttcccggaac aggaggccaa tcaccatgac taaactatat ttgctacttt tagttgcttg
    57721 tctgttcatc gtggcatata tcccgcgctt gttcccgatg ctttatttta ctcatcgaaa
    57781 agtgccatcc tggttcagtg actggatgaa atatgtaccg gtggcattat ttgcggcgtt
    57841 agcttttaaa gatgttttca ttactcatga acatctggat attgcttgga atatcaaaat
    57901 agctgcaatg attctagtag caggcgtggc ttacaaaaca cgttcgatgg cgttgtcagt
    57961 tttgacagga cttgcgtctg tctttttgct gtctatgctt taaactgttt tattttatta
    58021 aaaacagaga ttgcgacata agttgttcaa actgatctat ccgaactgca tttgcaacag
    58081 tcaattctgg atgcaagttt attcgtaggg ataaaaaaga ttagtgttgg cttatgatgc
    58141 aagctctttt ttattttagg aattagagag ggatttgtcc ttctatgatt ttttgctata
    58201 atagggaaga tgtttatttt atgaaagaat agttgaggga atttaaatga ataaaaaatt
    58261 gatgcaagga acattttggc taacctttgc taatcttcta tgtaaagttt taggggtagt
    58321 ctatttgatt ccctggctaa gtatgatggg gaacaatcaa gatggtatgc ttgctacgac
    58381 attgtataat gttggctatc ttccttatgg attgttcctg atgcttggta cagtcggatt
    58441 tccaaatgcg attgcaaaaa aagtagctgt agccacaaaa gaaggagata acgctgcttg
    58501 tcggctgatt tttagaagta cgatcaatat catgtttgtc atcggtatcg cctctgctgc
    58561 actgatgtat ttatttgctc ctttactagc cggaatcagt ccgattgcta acgttaataa
    58621 tgggatacta gctatcagaa gtttatgccc atctttgatt gctatcccaa ttcttagtgc
    58681 aatgagagga tatttccaag gaaaaaatga tctgcggcct tatggaactt ctttgattat
    58741 cgaacaggct gtgcgagtga tcgttatctt ggctggtact tattacttgc gcgtattgac
    58801 tgacggaacc atattagaag ctgtattgat cagtacagtc gcttctttct tcggtgggtt
    58861 agctgcgatt atccatatgt atatcgtcgg acgcagaaaa gacttcttcg aacttaaaga
    58921 ttttttgatt tccagccgtt atttcgaaag agaaaaccga tctgcctctg tctctatcat
    58981 tcgggaaaca ctaccgttta tttttgtcgg ctcagtcatt actatcgttc aaatgatcga
    59041 ccaagtaacg atgaaaccac tgcttcactt tttccgtcca gagatcgcag atcagcaact
    59101 ggaattctta ttcagccgtg cttctgtcaa tccaaataaa ttgacattga tcctgatctc
    59161 catggtagga acagtagcta taagcagctt gccaatatta agcacgatga agaaaaaaga
    59221 tcggatacaa atcgaaaaaa cagtaggaga tagtttttcg attgcgttat tgattcttct
    59281 tccttcattg actggaatgt ctttattagc tggaccacta tacacattgt tttttggcta
    59341 tgatcctgaa agtgtcggat atttccaaat ggctttgcta gcttctttgt ttttctcttt
    59401 attcacgatc ctatctacga tgcttcaatc tttgaatcac catcgttttg cgatccgatt
    59461 aactgtagaa gcaattatcc tgaaagtcat cttccaagta atcggattag gattagttgg
    59521 cggatacggg atgagcttat caaatggctt agcattcggt attgtctttg ttcgcggcta
    59581 tcactttatg tgcaaagaat accgaatagc acctttagca aagatcagca attttttctt
    59641 aaaaactttc cgctccaccc tgattatgct tgcagtatgt tttgccgttt tctttttgct
    59701 gaatcagcga ttgtcaatgg aatcaaaaag ctatgcaatg atttattgtg tggctgtagg
    59761 aagtattggc ggcttagtat ttttattttc tcaattcgga aaaaatgggt ttcgaatgtt
    59821 gaaaaatgtt aaacatcata gcagaagcaa agaataggtt aactaatcgt tttttgctgt
    59881 cacttgaaaa acggcaactc gtatgattac gcttgtcgtt tttctatttt ccaattttag
    59941 tttcatatct aatcttactt atgatatagt tataaggtct ggaaaaattc tttgaaacga
    60001 ggaaattaaa cacatgaaac ttgcagtagt tacagatagt tcggcatatc tgccggaacg
    60061 tatcaagaac catcccgact tattcgttat tccaatccct ttgattatgg atgggaaaat
    60121 ttataacgaa ggaatcgata tcgaagcgga tgaatattat ggacttttaa aaaacagtaa
    60181 agattttcct actacttcac aacctgtcgt aggggaagtt ttgggtttat atgaggaatt
    60241 gaaagagaaa gggtatgaca cgattttaag tatccatctt tcttcaggga tttctggctt
    60301 tgtcaacaca ttatttgcga tgaaagatga catcagcgga gtaacggtca ttccttatga
    60361 ctctaagatc actagtatgc cgatgggaca catggtagaa gctgccttag acttaaatga
    60421 tgaaggtcag tcccttgatg aaatacttgc acatatcgat cgaatccgag ataataccta
    60481 cgcttatctg attgtagatg atttgaacaa tttagtacga ggaggacgtt tgacaaatgg
    60541 tgcagcgctg attggcagct tattaaaaat caaaccgatc ctgacatttt ctgaagggaa
    60601 aattgtttta ttcgaaaaaa tccgttcaag taaaaaagca tttgctcgag ctgaagaaat
    60661 tattggtaga agaaataacg aaatacaacc gccagtcaaa ctttacgtga tacatgcaaa
    60721 taatttagaa gtagcgattg cggagaaaga gaaattgcag aaaaaatatc caaatgcaga
    60781 aatcgaaatt ggtcatttcg gaccagtcat tggtacacat ttaggtgaaa aagccatcgg
    60841 cattgccatt tcagcgcaat aattcctttt attcaaaaaa cataccaaaa atattccaac
    60901 atcatcttgg actttctaca tgttactaat gagcgtgtaa aagaacgaga tggtgttttt
    60961 atttctatca atgaagctct ttttctttgc aatcctcact tcaatcctct ataatgtcgc
    61021 atatagttta gtaaatgagt aaactactaa tttacttatt tactaaaaat aaaaggatga
    61081 ggagttaaaa taatggaaat accaacaaat ttaaaacata aaccagtaat cgttgtagaa
    61141 gactatgata aagttgatgg aagaaatgca ttaaatacgg atgcaaaagg gctttcactg
    61201 ggccttgctc agtggaatga tcgtggaaaa gtcgatatct caggcaaggt atggcgacat
    61261 actggagaaa aatggtcacg acagtcggaa gaattaccaa tcacacgtat tttagattta
    61321 gcgatcttag ctgcacaagg aagtttatac ttccaagatg cttatcgtta tgaaaaattt
    61381 tatgatccag aaaatccagt cgtagacatc attggattac aagggaaccg aatgacggta
    61441 gaagtagata ccaaaaatcc aaagatcgat gaagacatca ttttgtatta cgatacacta
    61501 caaaaagacg gagaactgat tggggaacga ttccgtattt taaaacgtct tttagatgag
    61561 ctaggatatt aatagacaga gatgatcaaa aggggaagag aagcatggag aatttctcac
    61621 taacagaaaa ccgaaccttt tcacggctag aaaagcaaat gatctggaag aaaccgaaaa
    61681 gccatcggac aagtgcagaa gaatggcgga tcgcctccga aatcaaaggg aattggcaag
    61741 atcctgaaat gaaaatcagt aacgtacttt tggaaggaga tgccgggtcg gggaagacac
    61801 aattagccaa agcactatct gctgatctgc aacttcccta tacaaaagtg acctgttttg
    61861 cagatatgga caaatcagat gttttcggag ccttattacc tgtagtagat tctaataagg
    61921 aacaagacca agaattacta gaagctattt accaaacaga tacacttgaa gctgtactga
    61981 cactgatcca gaaccattac ggcattgatc ctttttctgc taaagaaaaa ttagcagagc
    62041 tagttcaaag gatcgaagct gattatccag atgcaatcca ttacagctat tacccttctg
    62101 aaatcgttcg agcgattgaa aaaggatatt tattagagat tcaagagccg actgtcatcc
    62161 gagatgcctc tgttcttgtg gcacttaact cagcgctcga accaaatggg atgttgaatt
    62221 taccgacagg aatcattcgt cgccatccag attgcgtcat tgtgatcact acaaatcgaa
    62281 attaccaagg aaatcgccca ttaaatgaat ctttaagaga tcggatgcaa catgcggaaa
    62341 aaatggatct tcctgcctta gaagtcatgg cagaacgtgc gatggcgaaa actatgatcc
    62401 aaaaacaaga acttctctta aagatggcag agattatccg tttgttggat gaaactgcaa
    62461 aagctaacgc catcaaaggt gtagcaggaa tgcgttctta tttttattgg gcaaatacct
    62521 ataagcaagg acaagattta ctccagtcga tttatccaaa agttttgtat aaattatcca
    62581 ctgattcaga tgaattggca attcttgaac aagctttgtc tgacagtgga ttgctactgg
    62641 aacttcagga catattgaga aaagaaaaat ggggtcatcc tcaatctgag catacaaaag
    62701 gaagaaccat tagcgccgaa gaagcaaatg aacgaaacat cactgaaaat gctgaagaaa
    62761 ttctgcgctt agatgaaaag aaatcgttgt ctgctgaaga accaaatgag ggagaagaga
    62821 aacaagaaga aacaatagaa aaaaacgaga ttcctcctca aatgcaaaaa gaacgcagta
    62881 gcgatatcga aggaaaaaat caaaaagaac aacaaacagg agatgattct caagaaatgt
    62941 ctgcttctga catggaagca catgataagg aaatcaagaa gcaactgaat aaagaagcgc
    63001 gacaaatcat gaaggaaacg attcatgaaa aagaaggtct catcgttcac cgaccagctt
    63061 tccaaggaaa aacgccggaa gcacgtgagc tacacatgca agtaatgcca atagtagaat
    63121 cacttagccg gcaaatttta gagttactgg ataatgaaca aacagagacg taccaaaaag
    63181 gaaaatacga aggtcagcgg ttcaatgcaa gcaaagtagc atatggtgat ttgagaaatt
    63241 ttgacaaaaa gaacccgcca catgagcaac cttctttagc ggtagctgta cggatcgatg
    63301 aatctgggtc aatgatacgg gatgatcgga tcgaagcggc aaaaaaagca gcgattgcca
    63361 ttgccgaatt ttctaagaaa gtcgatatcc cattgttgat ttacggagat acagcagacc
    63421 gttcgtcaag agaaaagact tctttgtttt cttataaaga atttgaagac ggatttcaat
    63481 ggttaaatga aaaattagtc actatgaaac caaggcaaaa caatcgtgat ggtgctgcac
    63541 ttcgtttagc agcagaaaaa ttaaacagac aatccgcaac aaccaaactg ttgcttaata
    63601 ttagtgacgg tcagccaaaa gcgttgccgg attatacagg caaaaaagca aaagaagata
    63661 tccagtctgt tttgaaagaa tatgaacgtc aaggaatcct atttgtctcc gcagctattg
    63721 gccaagacaa agaagaaatc aagtcgatct acggtgaaag tcgttttgta gatattactg
    63781 acttgaatga atttcccaag caaatgattc aactgattgc ccgctatcta tgataaaata
    63841 aagctcaacg aacgcactta gcagattcgt tgagcttttc gtttctagtt accggtaaca
    63901 aggatacttc taaaatagaa tcggttataa tcaagaggga ccgttaaagg agtgaaacga
    63961 tatgatacaa gaacaaaaat ggtggaaaga agcagtaggc tatcaaatct atcctgctag
    64021 tttcaaagat agcaatggcg atggtcaagg agacatcaat gggatccgtg aaaaacttaa
    64081 ttatttgaaa gatcttggta tcggctttat ctggatcaat ccgatctatc aatctccatt
    64141 tgtcgataat ggttatgacg taagtgacta tcaagatata gaagagagat tcggaacgat
    64201 ggaagatttt gatttattgc taaaagaagc acatgaatta gggatcaaag tgattatgga
    64261 tcttgtcatc aaccatagtt cggatcagca tcaatggttt gaagaatcga aaaaatctaa
    64321 agaaagcccg tatcgcgatt attacatttg ggtcgacggg gtagatggca aagaaccgaa
    64381 taattggaca tcgattttcg gcggttctgc ttgggagtat tctcatgaaa ctggacagta
    64441 ttatctgcat gtttttgcaa aagaacaacc agacttaaat tgggaaagcg agaaattgaa
    64501 agaagaacta ttcaatatga tccgatggtg gttggataaa gggatagatg gatttcgttt
    64561 agatgcgatt tctcatgtaa aaaaagatga atattcagta aaagccacag agaacccttt
    64621 ctcacccttt caaaatgtct caggaatcga agaacatttg accgatttga aacatgtttt
    64681 tgaagagtac gatatcatga ctgtcggaga agcaagtggt gtcactgcag aagaaggacc
    64741 acaatgggta gggaaagacg gttatttcga tatgatattc gaatttgatc atatccatat
    64801 ctggcagcaa gaaaaagaag ggcaattaga tgtccttaaa ttgaaacttg cattaagcgc
    64861 ttggcaaact tcattggatg gtatcggatg gaacgcttta tatatggaaa accatgatgt
    64921 tcctcgtgcc gtttcagtgt ttggagatac tcgtcccgat ttttgggcta tgtctgcaaa
    64981 agcaatcgct atgatgtatt tctttttgca aggtacgcca tttatttatc aaggacagga
    65041 aatcggaatg acgaatatgc cctttgaatc gatcgatcaa gttgatgcag tagattcgaa
    65101 aagactttat aaaaggctgc ttgcggaagg taagacaaga gaagaagcat tggatatcat
    65161 tcgtgaaaca acgagagaca acagcaggac acccatgcaa tggacttcgg aacaatatgc
    65221 tggtttttct acacatgaac cttggctgat cactaatcca aataccaaaa cgatcaatgt
    65281 agaacaacaa gaatatgaac cagagtcggt cctgcaattc tataagaaca tgatccgcat
    65341 tagacaaacc aataaaggtc tgatatacgg ttcttataaa gaatatcttc atgaacatcc
    65401 acaactttat gtctatgaaa gatatttaga agatgaacaa tatcttatta tggtaaactt
    65461 aacagaatca cttgctgact atgaacttcc aaaagaagca gatcagtcct ggacattact
    65521 cttgtcaaat tcctctagcg gagaatttga agctaaaggc atactagcac cttatgaagc
    65581 tcggttatat aaaacaaaca agtaaaactt cctcgttaaa gttcattagt tggactttgc
    65641 gaggaagttt ttcagattgc tctgtcaatt actcatttca ataattcgct ctaggaccac
    65701 cgatatattt aggacgagag cgaagggctg tcacgtgagc ttgtccaagg gttttcaaac
    65761 gaggtctgac aggtacttgg acatgtttga tatgcatacc gattgatgtg tcaccgatat
    65821 cgataccggc ttgtccaact acgtgttcca cttcgacagg atcattgaac tggtcaaaag
    65881 cagcaacaga acaagcacct ccagcatgta gtgcaggaac aacagagaca atttcgaaat
    65941 tttttgcttc tgccactgtt cgttctacta ctaatgctcg gttcaaatgt tcacaacctt
    66001 ggacagctag cgcaatttct ttaggatcca acaactcttt taacgtacgg atgatccatt
    66061 gaccaacttc cgcactggaa ttttttccaa tatgtcctcc aacgacttcg cttgtgctgc
    66121 atcctaaaac aaaaagatca ccttttttca agtgtgcctc agccaatatg tcattaacta
    66181 tttcagtcaa ttggtctttt aattcttttt ctgacaactt cattttctaa aaccttcttt
    66241 ttcaattaat attgcttccg tgcttgaata cgttgaagac ttgcagttat aggaaaggca
    66301 agtcccattc caatcagatt ttggaagatg tttcctggta tagaagccac tccagccggc
    66361 caatcatata aaatccaacc ggcgatacca tagccgatga tcataactaa gcttcctaat
    66421 aaaagcccta atccccacat acgagtagaa gatttgctga aagaaccagc aacatatcct
    66481 tgtaacccgt ggatcactaa tgaaaaaact gcccactgcg gatatcctga aatcaaatcg
    66541 atcaatagac cactggaagc accaacaact aatccacccg ttggaccata aagaaaagca
    66601 gcggtataga tcccggcctc gcataatgtc acaaatccat tcgtagccgg tacagggatc
    66661 aaaattgtta atgaaagggc aactgtcaac gcaatcagca tagcatagat cgttaattgc
    66721 ttagttttca tttctcattc ctcctttttg aataccgcca gaccagacac ttccggtatg
    66781 atcgttcatc agcacgccat tagaaataca atgggcaaca aattttttac tttctgctac
    66841 tgctttggta aaatctgccc ctaacacaag attcgctgtt attgcagaag aaaaggcaca
    66901 accggccccg tttactgttg tagtgtcgat ttttttatga gaaaatacct cataatgatc
    66961 aattgaatag agaagatcca cagcttcatc tccagccatc cttgtgccgc ctttgatcac
    67021 aatgtttttt gttcctgaag cagtgagttc tttagcgaga tggatcattt ctgttaacga
    67081 agagggaaga gcttgaccag aaagtaaagc agcttctgcc aaattaggcg taatcatcgt
    67141 aacaagtgga aaaagttctt cgatgagctg cgaaatatac aattgattga tcacagcttc
    67201 agtttctttg aatgccaaaa cagggtctaa aatgattggg atattttgtt ttgtttttat
    67261 gaagtctcga acaaccatga ttgcttcact tgaattcaat aacccaattt tgataccatc
    67321 taatgaagtc attttatcaa tcgaaactaa ctgtcgttta agcaattcca aagatataga
    67381 ttccagcata aatcgctcat taatatctag tgcccctata gaagtcaagg cgcttagtcc
    67441 gaatagacca aaattctcaa aagtttttaa atctgtttgg attcctccac cagcaaatgg
    67501 atcagaaccg ccaattgtca agattctttt cataacattt tcctccatga ataagagtat
    67561 aacgaaattt tcaaaaaaga aaaacagcca attggcatat ttttatctca tccaatttaa
    67621 aaaaacaaaa agaaaagaaa gaccaaaatg gatctttctt tccttaaata gttaggactt
    67681 ttgtgatttt aattctttct cgatttgaag ccgttgcact ttcattgtag ctgttcgcgg
    67741 gattgcatca aaatcacgga tgatagggac gttcaagcga ggaagttcat ggacttgctc
    67801 ccaccatcga ttccaatcca tttctttatc aggagctaaa gcaataatcg gttgtggaga
    67861 atgattttta tcccttacaa tcactacttc tgccaaaaaa tctaacgaat ctaataaaaa
    67921 gtcttcaata gctaatgtac tattgatcgt ttcgatcaaa tccacttgcc gatctttcaa
    67981 aaataggtgg ccatgcttat ccataaaacc gtaatctccg ctatcccacc aatcgccata
    68041 aacattttcc tggaaccgtg catcttcctt gtaataagtc aaagcccgtc ctttagacag
    68101 taattgtata tgaccatctg tgttttcagg taggacattc ccaactgaat ctgtaatccg
    68161 agcgcttgtc atgtcttcca gtccaacgcc catatctctt gcatcacttg ttttcaaact
    68221 ctctagtgta tgagccttaa gaatcattgg tccgcattca ctttggccat atacttgaag
    68281 aaataccgca tcctgtgcca tcgacgttcg caaaaatgtc ttcattgtct ggttgtttat
    68341 agcatcaaaa gtggagtgat agtatcggat acccgaaaaa gcttctggat gctccttagc
    68401 agtaaaacgc cactgaacaa agttattcgg atgggtctct agtgcaattg gccggtgagc
    68461 ttccaacata tgaacaactt ttgaactttg tgcgtttgct aaaggcatca taggaaatcc
    68521 catggacatc aaagaagaga taccaatatt gaatctggaa tgcactggag agatgtgaaa
    68581 agcgattaat ttcttttccg aaatttttgt gaaaactgtt ttttgccatt tcgtccgcca
    68641 tcccatagaa tgagcagaat gacaaataag tttagggata ccagttgtac cagaagtatg
    68701 ggtcatataa gaaatttcat ttttgtctaa aaatgattga gaaacagaca aagcaggttg
    68761 ttccattaaa taaagaatag g
//
LOCUS       DAFEPQ010000008        64812 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.008, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000008
VERSION     DAFEPQ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.008"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(77..517)
                     /locus_tag="LP543_000572"
     CDS             complement(77..517)
                     /locus_tag="LP543_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBL2320761.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            complement(646..1572)
                     /gene="dnaI"
                     /locus_tag="LP543_000573"
     CDS             complement(646..1572)
                     /gene="dnaI"
                     /locus_tag="LP543_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBL2320762.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            complement(1572..2945)
                     /locus_tag="LP543_000574"
     CDS             complement(1572..2945)
                     /locus_tag="LP543_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBL2320763.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            complement(2968..3459)
                     /gene="nrdR"
                     /locus_tag="LP543_000575"
     CDS             complement(2968..3459)
                     /gene="nrdR"
                     /locus_tag="LP543_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBL2320764.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            complement(3771..4103)
                     /locus_tag="LP543_000576"
     CDS             complement(3771..4103)
                     /locus_tag="LP543_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBL2320765.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            complement(4155..4784)
                     /locus_tag="LP543_000577"
     CDS             complement(4155..4784)
                     /locus_tag="LP543_000577"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBL2320766.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            complement(4748..5584)
                     /gene="mutM"
                     /locus_tag="LP543_000578"
     CDS             complement(4748..5584)
                     /gene="mutM"
                     /locus_tag="LP543_000578"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBL2320767.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            complement(5629..8274)
                     /gene="polA"
                     /locus_tag="LP543_000579"
     CDS             complement(5629..8274)
                     /gene="polA"
                     /locus_tag="LP543_000579"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBL2320768.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            complement(8400..9083)
                     /locus_tag="LP543_000580"
     CDS             complement(8400..9083)
                     /locus_tag="LP543_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBL2320769.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            complement(9076..9561)
                     /locus_tag="LP543_000581"
     CDS             complement(9076..9561)
                     /locus_tag="LP543_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBL2320770.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            complement(9868..11202)
                     /locus_tag="LP543_000582"
     CDS             complement(9868..11202)
                     /locus_tag="LP543_000582"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBL2320771.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            complement(11333..12094)
                     /locus_tag="LP543_000583"
     CDS             complement(11333..12094)
                     /locus_tag="LP543_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBL2320772.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            complement(12110..12592)
                     /locus_tag="LP543_000584"
     CDS             complement(12110..12592)
                     /locus_tag="LP543_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBL2320773.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            complement(12612..14792)
                     /locus_tag="LP543_000585"
     CDS             complement(12612..14792)
                     /locus_tag="LP543_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBL2320774.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            complement(14936..15667)
                     /locus_tag="LP543_000586"
     CDS             complement(14936..15667)
                     /locus_tag="LP543_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBL2320775.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            complement(15736..16677)
                     /locus_tag="LP543_000587"
     CDS             complement(15736..16677)
                     /locus_tag="LP543_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBL2320776.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            complement(16857..17225)
                     /locus_tag="LP543_000588"
     CDS             complement(16857..17225)
                     /locus_tag="LP543_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBL2320777.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            complement(17836..19227)
                     /locus_tag="LP543_000589"
     CDS             complement(17836..19227)
                     /locus_tag="LP543_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBL2320778.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(19440..19826)
                     /locus_tag="LP543_000590"
     CDS             complement(19440..19826)
                     /locus_tag="LP543_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBL2320779.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(19882..20505)
                     /locus_tag="LP543_000591"
     CDS             complement(19882..20505)
                     /locus_tag="LP543_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBL2320780.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(20569..21237)
                     /locus_tag="LP543_000592"
     CDS             complement(20569..21237)
                     /locus_tag="LP543_000592"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBL2320781.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(21249..21689)
                     /locus_tag="LP543_000593"
     CDS             complement(21249..21689)
                     /locus_tag="LP543_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320782.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(21748..22419)
                     /locus_tag="LP543_000594"
     CDS             complement(21748..22419)
                     /locus_tag="LP543_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL2320783.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(22604..23002)
                     /locus_tag="LP543_000595"
     CDS             complement(22604..23002)
                     /locus_tag="LP543_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBL2320784.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(22999..23772)
                     /locus_tag="LP543_000596"
     CDS             complement(22999..23772)
                     /locus_tag="LP543_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBL2320785.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(23769..24452)
                     /locus_tag="LP543_000597"
     CDS             complement(23769..24452)
                     /locus_tag="LP543_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBL2320786.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(24619..25506)
                     /locus_tag="LP543_000598"
     CDS             complement(24619..25506)
                     /locus_tag="LP543_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2320787.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(25503..26372)
                     /locus_tag="LP543_000599"
     CDS             complement(25503..26372)
                     /locus_tag="LP543_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2320788.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(26509..27795)
                     /locus_tag="LP543_000600"
     CDS             complement(26509..27795)
                     /locus_tag="LP543_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL2320789.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            28068..28646
                     /locus_tag="LP543_000601"
     CDS             28068..28646
                     /locus_tag="LP543_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL2320790.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            28646..29575
                     /locus_tag="LP543_000602"
     CDS             28646..29575
                     /locus_tag="LP543_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2320791.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            29572..31308
                     /locus_tag="LP543_000603"
     CDS             29572..31308
                     /locus_tag="LP543_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBL2320792.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(31371..32207)
                     /gene="pheA"
                     /locus_tag="LP543_000604"
     CDS             complement(31371..32207)
                     /gene="pheA"
                     /locus_tag="LP543_000604"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBL2320793.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(32204..32713)
                     /locus_tag="LP543_000605"
     CDS             complement(32204..32713)
                     /locus_tag="LP543_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBL2320794.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(32706..34001)
                     /gene="aroA"
                     /locus_tag="LP543_000606"
     CDS             complement(32706..34001)
                     /gene="aroA"
                     /locus_tag="LP543_000606"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBL2320795.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(34014..35105)
                     /locus_tag="LP543_000607"
     CDS             complement(34014..35105)
                     /locus_tag="LP543_000607"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBL2320796.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(35122..36288)
                     /gene="aroC"
                     /locus_tag="LP543_000608"
     CDS             complement(35122..36288)
                     /gene="aroC"
                     /locus_tag="LP543_000608"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBL2320797.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(36290..37354)
                     /gene="aroB"
                     /locus_tag="LP543_000609"
     CDS             complement(36290..37354)
                     /gene="aroB"
                     /locus_tag="LP543_000609"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBL2320798.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(37387..38409)
                     /gene="aroF"
                     /locus_tag="LP543_000610"
     CDS             complement(37387..38409)
                     /gene="aroF"
                     /locus_tag="LP543_000610"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBL2320799.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(39068..40042)
                     /locus_tag="LP543_000611"
     CDS             complement(39068..40042)
                     /locus_tag="LP543_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBL2320800.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(40023..41906)
                     /locus_tag="LP543_000612"
     CDS             complement(40023..41906)
                     /locus_tag="LP543_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HBL2320801.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(41924..44149)
                     /locus_tag="LP543_000613"
     CDS             complement(41924..44149)
                     /locus_tag="LP543_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBL2320802.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            44264..45298
                     /locus_tag="LP543_000614"
     CDS             44264..45298
                     /locus_tag="LP543_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2320803.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(45365..46378)
                     /locus_tag="LP543_000615"
     CDS             complement(45365..46378)
                     /locus_tag="LP543_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL2320804.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(46456..46644)
                     /locus_tag="LP543_000616"
     CDS             complement(46456..46644)
                     /locus_tag="LP543_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBL2320805.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(47084..47212)
                     /locus_tag="LP543_000617"
     CDS             complement(47084..47212)
                     /locus_tag="LP543_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBL2320806.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(47215..48588)
                     /gene="rlmD"
                     /locus_tag="LP543_000618"
     CDS             complement(47215..48588)
                     /gene="rlmD"
                     /locus_tag="LP543_000618"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBL2320807.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(48699..49754)
                     /locus_tag="LP543_000619"
     CDS             complement(48699..49754)
                     /locus_tag="LP543_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBL2320808.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(49764..51194)
                     /gene="gatB"
                     /locus_tag="LP543_000620"
     CDS             complement(49764..51194)
                     /gene="gatB"
                     /locus_tag="LP543_000620"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBL2320809.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(51191..52663)
                     /gene="gatA"
                     /locus_tag="LP543_000621"
     CDS             complement(51191..52663)
                     /gene="gatA"
                     /locus_tag="LP543_000621"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBL2320810.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(52663..52968)
                     /gene="gatC"
                     /locus_tag="LP543_000622"
     CDS             complement(52663..52968)
                     /gene="gatC"
                     /locus_tag="LP543_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBL2320811.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(53074..55110)
                     /gene="ligA"
                     /locus_tag="LP543_000623"
     CDS             complement(53074..55110)
                     /gene="ligA"
                     /locus_tag="LP543_000623"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBL2320812.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(55127..57361)
                     /gene="pcrA"
                     /locus_tag="LP543_000624"
     CDS             complement(55127..57361)
                     /gene="pcrA"
                     /locus_tag="LP543_000624"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBL2320813.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            57516..59075
                     /locus_tag="LP543_000625"
     CDS             57516..59075
                     /locus_tag="LP543_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBL2320814.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(59186..61117)
                     /locus_tag="LP543_000626"
     CDS             complement(59186..61117)
                     /locus_tag="LP543_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2320815.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(61135..62049)
                     /gene="pfkB"
                     /locus_tag="LP543_000627"
     CDS             complement(61135..62049)
                     /gene="pfkB"
                     /locus_tag="LP543_000627"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBL2320816.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(62046..62798)
                     /locus_tag="LP543_000628"
     CDS             complement(62046..62798)
                     /locus_tag="LP543_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL2320817.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(62958..63167)
                     /locus_tag="LP543_000629"
     CDS             complement(62958..63167)
                     /locus_tag="LP543_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320818.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(63178..64506)
                     /locus_tag="LP543_000630"
     CDS             complement(63178..64506)
                     /locus_tag="LP543_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320819.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            64642..64797
                     /locus_tag="LP543_000631"
     CDS             64642..64797
                     /locus_tag="LP543_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320820.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
BASE COUNT        17838 a        14067 c        11488 g        21419 t
ORIGIN      
        1 tcccatatct ccttaaaaaa tatacctttt cgttctataa aacaacattt tcttattttt
       61 gaaaaaatgt tattggttat ttgttcaatg aaacatcaat aatcgaatct ccaggaactt
      121 gcggtgggat agacatcgtc atgatcggca tctcaactaa tgtaaattgg actttgtcac
      181 cgattttcaa ttcatttatg tccacgctta ttttttcagc agtagcattt aagatcactc
      241 cgtcattgct gaatgacgtt cccatattcg ctggatcttc tttggccatg acatcgacga
      301 gtttcaactg aagcgtttct tcttctgctt ctacttgagt aatactttca atcgttccga
      361 caaatatgct ttgtctctcc gttttcgtac tgcttgtttt tactgtttga cccgcatcaa
      421 tcgaatcaac agaacctgtg ttgctatctg cgctaccgca gcctgctaaa agaaaaaata
      481 gaccaactac agatatacta gaaaaaatag ccctcataaa aaatcctccg tttaagtttc
      541 tgcattactt gttgcggtga ttgtctccgg tcatttctgt ccatataatg attcgctgac
      601 ctgcttttac tactaggtca aatgtcagtt ggacttgtca actgttcaac cattgcgtcg
      661 gttgcgacca gtcatttcga tttcttttgt caaataacgt atgcgttcca ttatccgttt
      721 cgcttttagc ggctcttcat ctccacgttg agtgacggac aaatgctgtt ccaattcatc
      781 cattgtaaaa ttcgatgaga agaaggttgg taactgttcc tgcatccgat attgaaggat
      841 aacaccgaat acttcgtctc gtatccaact actcatcgca tctgcaccaa tgtcatcgat
      901 catcaaaata ggtgaacgtt tcactgcatc taacttttct gccacttgat cttttccgat
      961 tgcctgtttc atctcaacgg caaatgtcgg aaaatggact agcgtcgtgg tgaatccagc
     1021 aatcgctaat tctctcgctg ttgctcctaa taaatatgtt tttccaatac caaaacttcc
     1081 tactagatac agtcctttat gatacccttt tggatcagca gtatatttct ctacaaaatc
     1141 tagggcttct aagctcgcat atcctcgtcc atctgtacgt tcaaagtttt caaaagaagc
     1201 cgattgaata tcttttggca tatccattgc ttttactcgg cttctgattt cttcttgtct
     1261 ttgccgtgct aacaatgctt ccgttggtac ataagtgaca tccacataat gaaaattcaa
     1321 cgtcagcttc ggctcatagc cgggagcgat catcgttgga tcgttttttt gatatttacg
     1381 cttttcttgc acgaattcat acaatttagc gtaacttttt tcaatatccg cttgtgtcaa
     1441 ttcttcttgg tgtgcgctta gaaaagcttg aacatccgca tcatttaaga cttcagcgat
     1501 catttcagca tacttttctt gataattgcg tttgctgatg atcctcttca attctttgcc
     1561 tacgtcttcc actagatatc accctccttt tttgccaggt attctttaaa acggcggtca
     1621 atctctgctt ttttctcgct gctgatctgt ttttcatctt ttggctggtt gacccaatca
     1681 ggaagctttt cccgtcgaac atttcgcttg ccagtttgat aatttgtttg cttcttttgt
     1741 tttgattgtt ttgcttgatt acttatttgc cgaatgtgct ggatcgcttc ttctgctgtg
     1801 gcgattttct tttgtgccca ttcatttgcc actgtattta cataactggc atttaagcta
     1861 gcattattct tgatcactag tacataattc agcaaaacat tgatcacgct actggaaaga
     1921 cctgattgag aaacaagatc ttgcagtaac cagttttctt gtttagaaac atatccgcct
     1981 ttttctgact tgattgcttc cagatatttc atcggtggaa tgctctgtgc ttcttttatc
     2041 agctgaagcg ctgcgtttgt caaatgttcg tttgacgagg gttcattact gttttcagaa
     2101 accgcaactg tttcttttgt ttgcttcacc ggacgtctct gcattgccaa aatggctttt
     2161 tgcagttctt tttcagatac tttttgttca ctaaagtcat aagattgact gatcagttcg
     2221 ccaagtgtca attcatcgaa accgtaaagc tgatgataca tgtcgacttg tttgatgatt
     2281 ggatcatcaa gacttgcaat atgttttttt gccagccaat cattcaaaaa ctgccaatcg
     2341 aatgtcgaat ctgcttcaag taatgctact gattcagatt cttcaaaaga tttattcagc
     2401 gattcgatgt tttcttgatt agcagcgtac actgcttcat tgaagcgata aacatctaaa
     2461 aatttttttg tgattttttg atatccctct gctgtatgcc ttttaggttc aaagaattgg
     2521 gtaagcttct ggaatcgtcg ttcaccaact ttttccagaa gaagaaaagc cagaacagag
     2581 tctttgaaaa atgctttagg aatgagtggt tctaacagct cataaataaa acgaggtccc
     2641 agatcactat cttctttaaa ataaacatcc agtaagccga ttccttctag cttttttctt
     2701 gcatcataaa attgcgggat accgcagcct agactactaa gcagatctgc atgcatccct
     2761 tcaggacctt tgccagattc ttgagagatt tctgataaca gggtgaaata gatactcaag
     2821 gcttctccgc cgattagtgg ctgataaaga tacagcaagg cgttcttgct gtcttctctc
     2881 agcggagccg cttttcttcc ttggtaaata ttttttggtt gaagctcttt ccaagcattt
     2941 tccaaggaaa tgcctccttt ttgtttctta attgttttct ttattattct ctttattatt
     3001 ttcttttgct ttatccacga tatcttgcaa ctcttttaag aaaacagaca tatctttgaa
     3061 ctggcgataa acacttgcaa aacgaatata agcgatttcg tctaaatcaa ctaaatcttc
     3121 catcacatat tcaccaatta gattcgtcga tacttcgttt tctccgagac tgcgtacgcg
     3181 gttttcgaca tggttgacga tttgttccat ttgttccata gctacaggtc ttttttctgc
     3241 tgaacggatg agtcctctta aaattttatc gcgattgaac tcttcgcgtt ccccgttttt
     3301 cttgattact aataacggag cagcttcaat tcgttcaaat gtagtgaatc ggaacgaaca
     3361 attctcacat tcgcgacggc gacggatcgc acgtccatca tctgcttgac ggctgtcgat
     3421 tactcgtgaa ttattatgtt gacatctggg acatctcatt ttgtctcacc tcttgcatcg
     3481 cattttatat aaaggatgtg tctttcttta caactagttg tattataaca cgaaacaaat
     3541 cacaaacgcg actgtcctac ccaaaaaaca cggatataag aacaattttt tctactttca
     3601 tgcgtaattg tgtcccctgt ccttactaaa tttcaatttt ccaatactga tcaccttgat
     3661 tactgagaaa taacttacac aaaagctgat ttagtcatct agcacaaaaa acttgaagca
     3721 gatgaacacc ttctcatctg cttcaagtca catctcgatt ttttactatt ttataacttc
     3781 tcttcgattc ctgtatagtg tttcattcgc tgttcctcga ttttttgctc atcgggaatg
     3841 tggtcacttt ctgggagcgg tgttttacta cgatcaggtg tcggattttt tcgttcttga
     3901 gtcaaatctt gccgcttcgc ttcttcttct gcctgctggt cttctatttc atcgattctt
     3961 gcttggtact gcttttcttt ttcttctgct gtttctgatt gcgtacgacc ttcatcgatt
     4021 agattttctt tattgagcat ctcgccgatt ttttcttttg cgttacctac gactttatcc
     4081 ttttgatttt ttaatgatcc catcgctatc cctcactttc tatatactca ggatagcaaa
     4141 attgacacag atactcaaag aaagcgatta gcaaaaagcc aattttcgat ttgtcccaca
     4201 agtttttctt ttgttccatt gttgtcgaac aaaatatccg cacgctgctt tttttcttcg
     4261 attggaagtt gactactaat tctctgctgc gcttgttcta gcgtcagttg attacgtttc
     4321 atcaaacgct gaagctgagt ttctggcgta gtataaacga cagctactgc atccatatag
     4381 tggtcataat gaccttcgta gagcaatggg atatctacaa ttactaaagg atgattggaa
     4441 cttagagagg cgatttgatc ttttatcgct tttcggataa acggatctaa tagctgatca
     4501 agtttttgcc gtttttcctt tgatggaaaa acaatcatcc caagtttccc tcgatctagt
     4561 tgcccatcag gctgcaatac ctcttggcca aacactgata caattttcct cagagcaagc
     4621 gttccaggct caacagtttc tctggcaatc acatcgccat cgatcaaagg aaatccatga
     4681 gactgaaaga ctttggctgc cgtactttta ccggtagcta ttccacccgt caaacctaaa
     4741 acaaatccta ctttgcgatt tttagccttt gacaattcgg acaatagtgc gtcccccttt
     4801 gtgccacttt tgttttcacg atcggcgttc cacaacgtat acatggtttg ccagtttgct
     4861 gatagacatg cagtgctact tgaaatccgc ctgattctcc taatgcattc aaataactgc
     4921 gaatcgtggt acctccggct tctattgcac gatccaaaac atcaatgatc gatagacgta
     4981 attgttctac ctctttgggg cgcagtgtgt tcgccggttg ttctggatgg atcttcgctt
     5041 cccataatgc ttcatctaca taaatattac ctagaccagt cactagacgt tggtctagca
     5101 gcaatggctt gattgctttg tgggactttt tcagtccatt tgcgaaatct gctaataaga
     5161 agctatctgg caaaggctca ggtcccaatt tcatgattcc tcggtaagtt gcacttgctc
     5221 ctttttcgac gattgtcata cgaccaaatt ttcgtacatc gttgtagcga agttggcttc
     5281 catcctcaaa aaagaaaagt acatgcgtat gtttatcgat tggtgtattt tctttcgtaa
     5341 attgatattt tccttccatt cgcaagtgtg agatcagttc acaatgatcg agatgaaaaa
     5401 ttaaaaactt ccctctacgt ccaatggatt ggatggtttc gccaacaaga gaagcctcaa
     5461 agatgggtgt ctctggttgc tcgatgatct tgggccacaa gacctgaact ttttgaatcg
     5521 tttttcctac tactaaccgt tccaatcctt tacgaacggt ttctacttct ggtaattctg
     5581 gcattttttc ttttcacctt cattatattg ctctgtctta aacaatgttt attttgcttc
     5641 gtaccacgtt tttccccaac tgctgtcagt gatcagtggt acatgaagag aaacagcttg
     5701 ttccataact tccttcacca atttatctaa tgattcaagt tctttttccg gtacctcgaa
     5761 gacaagttca tcatgaactt gtaacagcat cgtagcttga agacctgttt cttttaatcg
     5821 tttatccaat tcgatcatcg cgattttcaa aatatcagca gcacttcctt ggattggtgt
     5881 attgatagct gttcgttccg caaaagaacg gatattgtaa tttcttgaat tgatgtctgg
     5941 cagatatcga cggcggtgat acagcgtttc gacgtagcct tggtcttttg cttcgcgtac
     6001 gattctttcc atatattctt tgacacctgg gtatttttca aagtacgtat cgatgtattg
     6061 ctgtgctgct tttcggctga ttcctagatt ttgtgaaagt ccgtaatcag agatgccata
     6121 cacgatacca aaattcactg cttttgcctg cctacgcata tttggtgtga catcctctgc
     6181 cttttcgatt ccaaatacac gcatcgctgt gctggcatgg atatcttgac cttctaggaa
     6241 ggcagctttc aaatgttcat catttgagat atgcgcaagt acgcgtaact cgatttgtga
     6301 atagtcagaa gaataaatca gccagtcttt ttctctagga acaaatgcct gacgtatctt
     6361 tctcccttca tctaatcgaa tcgggatgtt ctgcaaatta ggatcaacag agcttaatcg
     6421 tcctgtttgg gtcaatgtct ggacataacg ggtatgcact tttccatctg attggatcac
     6481 tttcagcagt ccctcgacat aggtagattg aatttttgca atttgacgat aggtgaggat
     6541 atcctctacg attggtgctt gttcacgaag ctgttccagt acatctacgg ctgtggaata
     6601 tccagttttt gtttttttga taacgggtaa tcccattttt tcaaataaga tcactcctaa
     6661 ttgctttggt gaattcaaat tgaattcttc gccggcttct tcgtagattt tttgttcgat
     6721 ctctcttaag cgagcagaga actcgccctt catttcttgt aagcgagtcg catctacttt
     6781 gatcccgctt atttccatct ctgccaaaat gatagacaat ggcagttcca ttttccggaa
     6841 aagatcttct tggttttttt ctgccagttc ttggctcaat tttggtgtca acgcattgat
     6901 tgcagctact tttcgcgcta aatgagcaaa aaagagttcc tcttcttctg gtaaaccttt
     6961 tttcgcccct ttgccgtata cagcttcatc tgattggata tcgttgtatc cgtaatgagc
     7021 agctattcct tcgatatctg tgcttttttc attcgtatcg agaaggtatg cacctagcaa
     7081 gacatcaaaa tcgattcctt tcgtttttcc agtatagcgg tttaaagcaa cataagttcg
     7141 tttcgcatca tatacttttt tcagccgagt cggatcagtg atccaagaat ggaacgctcg
     7201 gttttcgaat acagatagat cgttcgtgac ataaattttc ttttctgtcc cccacgctac
     7261 tccgacgatt tcctctgtat gataattgtc gcccatcatt tccacgtaca acgccatatc
     7321 cgtagtaaac atttcctctt tgaattcatg aacgacttca aacaaaatat catccatttt
     7381 tacttcttct tctacgatat tcaatttaga aaggaactga ttgaaatcca tttctttata
     7441 aaacggaacg agtttatcga gatttttccc ttcatacttc aatgagtcga tagagacatc
     7501 cacaggcgct gaagtattga tggtcgccaa ttttttcgat aagaaagcct gctctttgtc
     7561 atttatcaga ttttctttca ttttgctttg cttcatttca tcgatatgtt cataaattcc
     7621 ttcgactgag ccatattgtt tcaatagctt gatagctgtt ttttcaccga ttttagtcac
     7681 accggggata ttgtcagagg catctccggc tagtcccttc atgtcaataa tctgttttgg
     7741 cgtcaaccca tcatattttt ccgctacatg ttctggtgta tagctttcga tatcgctgac
     7801 accttttaca gtgatatcca cttttacttc tttactagct aattgagtca aatcccgatc
     7861 acctgaaagc acgacgacat caaattgatc tttgtctact ttttctgcca aagtaccgat
     7921 aatatcatct gcttcataat tgggcaattc gtaatactgt acccctagtc ctgtcaacaa
     7981 atcacgtata tatggcattt gttctttgaa ttcgccaggt gttttggatc ttccaccttt
     8041 gtactccgca tacatttctg tccgaaaagt cgtttttcct gcatcgaacg caaccagaac
     8101 atgagtcggt tgctcttttt gcataacatt ttcaaacatc gtattgaatg catagattgc
     8161 atttgtatga agaccgtttc tatttttgaa acgttcaaga gaattgtgaa gcgcaaagaa
     8221 tgcgcgaaaa gccacactgt tcccatcaac gagtaataat ttattttttg tcatcttctt
     8281 aactccttac tgtaaagttc ttattcattc taacaaagaa tcctgcggaa tgcgaaaaga
     8341 aaaagcggga ccataccaaa taattcggta tagccccaac tgatgaattt ttttcttttt
     8401 tagttgttcc gtccaaagat tctcagaaac gccaagaaca agttgataaa gtccaaatac
     8461 agcttcaatg ccatgaatac agcaattcca ctatgtgctg ttccgtttgt ctggacatag
     8521 atattacgga tgctttggtt atcataagct gtaatccctg caaagatgac gactaacagt
     8581 agggagatga acagctcaac tacgctactt ctcaaaatga agatgttcaa gactagtgca
     8641 atgatcactc cgattaaagc tgcctgtcct gctcttccaa ccccgctaag atctcttttt
     8701 gttgttactc cgacaacaga catccccatg aacatcaaag tagctgtcaa gaaagcttgc
     8761 gttactgttc caatatcgta gtatgcaaga gtaaccgcta atgttatgcc attcaaaaga
     8821 gagtaggcaa taaatccacc gattgctaag ctcggattct tttgtgcttt tacgcctaga
     8881 accattacta aaatcagttc aacgatccac agacccacaa agcctagtgg aaaattattg
     8941 ataaaagttg ctaattgcaa aggaaatacc caaagagcca agtaagaagt gactgcacta
     9001 agggcaagtc ccatacctaa aaatccataa acttttgcat aaaaacgatt gatacctgac
     9061 attgatgtac ttggattatt cattcaattc accttcactt tcgattgcta tttccaattc
     9121 aggaacttgt ggcggttgga ccataacgac agcatccaac acacgatctt cgttcgtatt
     9181 ctccgcttct acagaaatcg tcaccacatc cttcatctta tctactttta tgacctcaaa
     9241 ctgaaaagtc agtgtttcat aatggaaaac cggttcaata aaattgacag aaaaattgac
     9301 aacatgtgat cctggtccag gaagatgctt tgaaattgca cttgtgatga tccccatcag
     9361 catcactgat ggaacgattg gctgttgata ttctgttttt tgtgcaaagt catgttggat
     9421 atacaaagga tttgcatcat tcgtcaatcc caaatacagc aaaagctggt tatcttcaat
     9481 agattctgtt aaggataacg agtcgccttc ctcgatttca tttattgttt ttcctagttt
     9541 tctcggctta cctatgttca agatagaaac cctccagttt atttttaatc atacattatt
     9601 gtaccaaaag tagcgtaaaa ggcaagttct gctcccttga tttaacagat tcctatagtt
     9661 tacagaggtc gtgaaaaagc tgtcctgcat caaataataa gaacagccaa gaaaaaacag
     9721 cttctcatgt ttttgatttg attgggctta ttaccgcaga tgcaagcttt tgaacaccgt
     9781 ttatcataag ttgcaaaaaa gggggcttgg caagattttt gccactcccc ctctttctat
     9841 ttttataagg acactgcagt aattgtatta cagtacattt ttcgtcgtgc tgcttaaaag
     9901 tttttcatac gcttgttcaa atttttgaac atctccagca cccatgaaga ttactactgc
     9961 atcatgataa tctaacaatg gagatacatt attttctttg atgacttctc cacctttttt
    10021 gattttcgct ccaaggtctt caatcttaac gtttccttgt tcttcccgag ccgaaccaaa
    10081 aatatcgcat aagtaaactt tatcagctaa atcaagtgct tctgcaaatt catccatcaa
    10141 tgcaatcgta cgcgtaaacg tatggggttg gaaaactgca attatttctt tatctggata
    10201 tttttggcgt gcgccatcaa tcgttgcttt gatctctgca gggtgatgcg cataatcatc
    10261 gactacggtc atgtctgcta cgatcttttc actgaatcga cgttttactc ctgggaaagt
    10321 cagcatttct tctgcaactt ctttcagatc cagtttttcg aaataagcta ctgcaataac
    10381 acctaaagca tttaagatat tgtgtttacc gaatgctggt acagtgaagt gcccaacaaa
    10441 ttcatcccca tgatagacat caaaagcaga accagaagtt gtgcgttcga tgtttcgtgc
    10501 ttggatatca tcgttttctg tgacaccata ataataaatc ggcacatttg cttttaattt
    10561 gcgtaaataa gcatcgtcac catacgcaaa aatagctttt ttcacttgtc cagccattgt
    10621 ttggaaagct gtaaatacat cgtcgatact tgtataataa tccggatgat caaaatcgat
    10681 attggtcatg attgcatagt ctggtgaata ggcaaggaaa tgacggcgat attcacaggc
    10741 ctcaaatgaa aagaattccg cttgcggatc accatgtcct gttccatctc caattagata
    10801 acttgttgga cggacaccac tcaatacatg agagagcagt ccagtcgtac ttgtttttcc
    10861 gtgagagcct gttacagcga tactcgtata attttggata aaatgaccaa taaagtcgtg
    10921 atagcgaatc acttccaaac ctaattcttt tgctcgctgg atttcttcat gtgaatcagg
    10981 aaatgcattt cctgcaatga tcgtcatccc tggttttaca ttatctgcgt taaatggcag
    11041 aatcgtgata tttgcttttt ctaaatctct ttgtgtaaag aaatattttt caatgtctga
    11101 tccttggaca tttagacctt gttcatgtaa aacaagagcc aaggagctca ttcctgaacc
    11161 tttgatacca acaaaatgat acaatttatt ttgattttcc atttgaatcc tcttctctta
    11221 tctagcgtcc attcttttag ctattacatt caacacgctc ctcattatct tataaacgat
    11281 tgaaaatttc aaccaaattt atttggaaag gaggattcgc ttgctaaaat ggtcaagtac
    11341 gagaaaaatc cgtttttatt gactctttaa ttctggtgta agaagaccaa acttcctctg
    11401 atatatcaat gtgaggaata aaatcttttc ctttcggacc atagccgttc tcatcgtcat
    11461 ggagacgttc catttctcct aatactagcg catggaaata ttccttaggc cattgatgat
    11521 tcagttcttt tgctgtaaag atgatctctt cagaaaagtg aaggatttcc ggcacaaatg
    11581 gcacagcatt cgcgaaaata gtttgttcat tttgccatac tttctcaaaa gttgctcgag
    11641 tcctgcgttg cagtgtcgga tcattcatca ataagatttt ttctggtaca gctcctaagg
    11701 aatgaagaat ctccagtgaa aaagtggcgt tctctccaga atttgtagac ttcgattcta
    11761 tcagaaaagc cttatcgggc aaatcataga cttcttttag atactgacga cagatttcac
    11821 tttcactcat gccatcttca aaatgaaatc cttgcttttc aaaattctca taaaggattc
    11881 tagtcgcatg ccccacccct ccgaccaaaa acaattgatc tacttggcca tttaaataca
    11941 tttgtgctgc tttatcagct aagataggaa gacagtttcc agctaaaata agtgtaggtg
    12001 ccgtcagttc ctcaaaattt ccctcttcag gtgcggctaa aaaatttaat agctgattcc
    12061 agtcttttag attcctctgc tcactttttt ccaaaggcct tctatccttt catctgattt
    12121 tttgtgcttc attaaagtct tctctttttg tctcaatcca tttgatttct ccatgacatc
    12181 ttccacaacg atatttgcgt acatcgattc ttctcttgcg gcagatcgtg gcagaacaat
    12241 cttggcatcg ataatactcc atctttatct gtttcttcgt caaggctggt gcatagcgca
    12301 accctcctgt ttttttcaaa agatacttga aatccatatc tttatgctga taccctcttc
    12361 cagctagatg aagatgataa tggcaaagtt catgtttgat gattcctgca atcgtctctt
    12421 cgtctgactc ttcaaatagc ttcgcattaa aatctaaatg atgatcgttt aaatgatagc
    12481 gcccgccagt tgtacgtaag cgtggattga acattgcttg gtgcttgaaa ggaagctgga
    12541 agctttttaa tgatatctct tctactaact gctgcagttc ttcttgtgtc aatttttgtt
    12601 actcctcatt gttactcttc ataaggggaa agcatcgtca aactgattct gccttttttc
    12661 gtatcgactt gttctatcca aactgttacg atatctccta ctgacacgac atctgtagga
    12721 tgcttgacga attttttgct gagtttcgat atatggacaa gtccgtcttg cttcacaccg
    12781 atatcaacaa atgcgccgaa gtcaatgaca ttacggaccg ttccagtcaa ttccatccct
    12841 ggtttcaagt cttccatact caatacatct gtacgtaata agggcgctgg catctcatca
    12901 cgcatatctc gtccaggttg tgtcagccca gctaaaatat cggctaacgt ttcttctccg
    12961 actgataggc tttcagccag tttctctacg gataatcgtg tcaatttttc tactgcttct
    13021 tctgtaccaa gctctttttc tgaaagttgg actgaagtta atatttcttt tgcaatagaa
    13081 taactttcgg gatggatacc tgtgttgtcc aaaatgtttt tcccaccagg gactcgcaag
    13141 aatccgattg cttgttcata tgcttttggt cctaagcgtg gcactttttt caattgagtt
    13201 cgtgcggtga attctccgtt ttcttcacga taaatgacaa tgttttgggc agttgtttta
    13261 ttcaatcctg atatatgctg caagagctgc gggcttgctg tattcacatc cacacctact
    13321 tggttaaccg ctgtttccac aacaaaatcc aattgttcag ccaaacgttt ttgagaaacg
    13381 tcatgttggt actggcctac tccgacagct ttaggatcga ttttgactaa ttcagctaaa
    13441 ggatcttgca ggcgacgggc aatagaaaca gcacttcttt cttcaacttg tagatgcgga
    13501 aattcttttc gtgcaatttc gctagcggag taaacagaag cgcctgcttc gttgacgata
    13561 gcgtaatagg ttttatggtc ggcggatttc aactgctcag ccacaaaaag ttcagattcc
    13621 cgactggctg taccatttcc gatagctacc atatctactt gatatttatt aattaattgg
    13681 ataaaagctg gtccagcagc ttctcttttt gcttgcgctg caggtttatg aggataaata
    13741 acttcgattg ctaaaacttt ccctgtcgca tctacgactg ctagtttaca tccagtacga
    13801 taagccggat caaatcccag gacaacttta ccttttagtg gcggttgcaa caaaagatta
    13861 cggaggtttt cgccgaaaat agcgattgct tgctcatctg ccttttcagt caattcattg
    13921 cgcagttctc gctcgattgc tggctgaata aagcgtttat aactatcctg ataggcttct
    13981 ctgacgaatg aagaagaagg agaggcagga tttttgacta tttggcgatc caagtaagct
    14041 aaaattgctg cttcatctac ttgaaagaat actttcagaa tatcttcttt ttctccacga
    14101 ttagtagcta atattcgatg agaaaccatt ttatggacgg gttctgcaaa gtcataatac
    14161 atttcatata cgcctttttc atctgcttgc tcatccttga cattgctgac atacatacct
    14221 ttgttatacg tataagaccg aatccaagtc cgaaattttg cattatcgct gacttgttca
    14281 gcaataatct catgagcacc gtgcaacgct tcttcggcag aagagacttc tttttctttg
    14341 tcaatatatt tttctgcttc agattgcacc tctccatctg taagctgcat cagccacttc
    14401 gcgagtggtt caagtccttt ttctttagca atcgttgctt ttgtccgtcg tttttgtttg
    14461 tatggacgat acaaatcttc cacttgttgc attttcacgg cttgggtgat ttctgcttct
    14521 aactcgggag tcagtttccc ttgttcatcg attagccgaa tcacttcagt tttacgtttt
    14581 tctaagtttt caagataggc ataccgctct tcgatttcac gaatctggac ttcatcaaga
    14641 cttccagtca tttcttttcg gtaacgcgcg ataaaaggaa cagtgtttcc ttcatttaac
    14701 aaattaagta cagtagtcaa ctgcttagga cgatagtctg aaagttcttt ttgtacaagc
    14761 tgaatgatcg tctggttcaa attttccgtc attaaaatgc ctcttttctg ctaaaaattg
    14821 cttctttatt ctatcatact ttttatcctg ggttaaacta ctgaaagaaa caatattctc
    14881 tataaaaata aaaagagccg caacaaagtc acagctcctt cattctttcc attagtcaga
    14941 ttccatgcca aggtccttcg ttgttctgat attcaatatc ttgaagaata ccttccttgt
    15001 caaaaacaac cccatgcaat gaccctccga atacagcccc accgtccatt ccgattttat
    15061 gatcctgaat ccagaggtct gttgtctgca tatctccatg gagcattggt gtaatcgtat
    15121 gtccaaatac aattgtctta cctgtattgt ttttcccttc atgaaatggt tcccttatcc
    15181 aaataaagtc tcttagactt gtcttacgcc agtctttctt tgtcaaatcc acaccggcat
    15241 ggacaaaaag ataattttcc cattcataat acaaggggcg ctggactaaa aatttaatca
    15301 gttctgaata tctcgattgg atcatcatcg agatttcagt tggggagtat tcctccgatg
    15361 cccctgggtg aatcaggctt tcaaacgttt cttttccacc atttctcata taaggcgcat
    15421 accagtcctc cggtgattgc agaaattgca aaaaatattc ttcatggttt ccccggaggt
    15481 aaatggctcc tgtttcttcg actaattgct ttcctaataa aaaacattct tttgtacgag
    15541 gacctcgatc attgagatcc cctaataaaa caagctgatg gatctttgga tcataatagt
    15601 gaataagttt ttcaaaaagc tcatgtttgc catggacatc cccaatagca taaatcaatg
    15661 atttcatgtg gatcaccatc ctcaacgcta gtatagcgca tattggaaat ggtcgacaat
    15721 caaaactatt gttttctatt taattaaagc tgtaaaccgc agttctgctt cttctggaga
    15781 gattccatgg tcttgaacat agcgattgcg cggatgtttc gcacactcta tgctacatgc
    15841 tcctaaatgt ttttcttcat tttcaatgga agcaagaatc tgacgattgc actcaggatt
    15901 gctacaattg atatatcgtt cacatggtgt tccgtcaaac cagtcgcgac cgacgatttg
    15961 tttatctact tggttgatat cgacagcgat ccgttcatca aacacataca tcttgccgtc
    16021 ccataattct ccttggactt caggatcctt accataagta gcaataccgc catgcagctg
    16081 tccgacatct ttgaatcctt cacggacaag ccaaccagaa aacttctcgc aacgaatgcc
    16141 accagtacag taggtcacta cccgtttttc cataaattct tctttgtgct cgcggatcca
    16201 gtctggaagc tctcgaaaac tgcgtatatc tgggcgtaca gcccctcgaa aatgtcccaa
    16261 gtcatattca taatcattcc gtgcatcaat caccactgtc tgttcatcta aaatagcttc
    16321 tttaaattct tgcggtgaaa gataggcacc tgtcacttgg tttggatcaa tgtcctcttc
    16381 tagctttaaa cttacaagct ctggacggta tcttacaaac atcttcttaa aggcatgtcc
    16441 ttcgcttgga tcgatcttga acatcgtatc agcaaatcga ggatcagctt tcattgcttt
    16501 catataggca gtcgtgtttt caacggttcc agacacagta ccatttattc cttcttcagc
    16561 tactagaata cgacctttaa gatccaaaga tttgcaaaag gctaaatgat ttttggcaaa
    16621 ttcctctggt tcttcgattt ttgtataatt gtaataaagc agtacttgat aatccatgta
    16681 tttcttcctt ccttcttttc gttcctaaac agtataaaga aggcgttcgc atttgacgat
    16741 ttatttgctt gtgtataatt acgcatggaa agttttttgt ttacataata ttattatttg
    16801 aattaaaaag acttcgcttt cctgtttcgc acagaaaaac aaagtcttta tttctattat
    16861 ttacctgtaa ccgcaaagta gttgccatcg ggatcagcaa agttaaagac atattcttct
    16921 ccaagctgta ccatatcacc taatgtgact gattgcgtct gcattttttt atatagtcca
    16981 aagatatctt tactgtaaaa catcaaagat ggggcactag tagctgtgcc aggtgaatgg
    17041 gactcaacaa atgctcgatc atataacaga aatgaggttt gactgtcctc aactcctact
    17101 tcaattataa gcgtcccatc catttcttga cgatcttttt ccgcaaagcc aactgctctc
    17161 caaaacgttg catttttttc tacatcgtcc acataaagca tgatttttat ttcattcgta
    17221 aacatctgtt tcctcctaaa cgatctgttc taacattctc aactttgtca gtttaccata
    17281 ggaaaatggg aaaatctacg aaagctctca acagaatcgt cctacttaaa tacgcttttc
    17341 tttaataata aataagttgg ttgtttttgt tatttgcgct ttcacttttc ggaataaata
    17401 atgtgtattt atatggaata aataaaaaaa gaggtcgtga ctaaaaacaa gtcacaacct
    17461 ctggtaaacg gtgttctcct gacaactcct cggtcacaag acacaatatt caacaaacat
    17521 gagaagctat tttttgaaat tgctctttgt gactcagagt gaaacggcag gctcacgacc
    17581 tctttcttaa acggtgttca aaagcttgca tctgcggtaa taagctcaac caaacgtaaa
    17641 atatgaggaa ctatttttgt ttggctgttc ttattacttg atgcagggca gctttttcac
    17701 aacctcttat tcacggtgtt tcagaagtag cttctcggaa ataagccgaa atttcacaaa
    17761 aatttgagaa actattttta ggaaacttct tcttatttct taaagctgac cacttctatc
    17821 acaacctctt tttgactatg cattcacatc gatcacttta gaatcatcat tgatgacatc
    17881 gcgatctaaa tctttgatgc gtgcacttgt cacgatacca gctaccatgc taccgctgac
    17941 gtttactgct gtacgtgcca tatcgattaa tggttcaaca gagataacta acccaacgat
    18001 tgctactggc aagttcattg cccctaaaac gatcagtgaa gcaaatgttg cgcccccacc
    18061 gactccagct acaccaaaag aactgattgt tacaattgcc acaagcatca aaataaattc
    18121 caaactaaag acattcactc cagctgctgg tgcgacgatc gttgccaaca tagctggata
    18181 aacgccagca cagccgtttt gtccaataga caaaccaaaa cttgctgaga aattagctgt
    18241 tgcatcatct actcctaaag cttttgtctg agtctcgata ttcaatggca aagctccagc
    18301 acttgaacgt gaagtaaacg caaagctcaa taccggaaag gcttttttga aatagttgat
    18361 tggattgact tttgcaccaa tcaaaatcag tgagtggaca atcaaaacag caagcaatgc
    18421 tacgtaagaa gcaacgacaa atttacctaa attgacgata gctgcaaaat cacttgttgc
    18481 caaaacgttt gtcatcaaag caaatactcc ataaggtgtc aaacgcaaaa caagcgtcac
    18541 gatccgcata atgattttat acagactgtc gactaaatct gaaaagaatg ctgcttcttt
    18601 tggcgctttt cttttcacgc ctagataagc gattccgaca aatgccgcga aaataacgac
    18661 tccaatcgta cttgttgcac gactacctgc taaatcagca aaaacgtttt tagggataaa
    18721 ggataaaatc tgctgtggaa tcgataagtt ttcaactgtt tcctgacgtg ttgccagttc
    18781 agagattcgt gctgtttctg cagctccttg tgtgaattta gctccttgga gtccgaacag
    18841 cattgttgaa ccaattccga tcaatgctgc aatagctgtt gttccaagta aagaagtcaa
    18901 tacagtcgca ctaattttcc ctagcttttc agaaactttc atttttgtga atgcaccaat
    18961 gatcgaaata aacactaaag gcataactag catttgcaag aatgatacat atccttgacc
    19021 tacaatcccg atccattcca ttgattgagt aacgactttg tcgtctacac caaagatcaa
    19081 ttgaagcaga ccgccaaaga cgataccaac tcctaatgct gtaaagacgc gtgttgaaaa
    19141 ttttgtatgg cgcttttgca tacgatagaa cacatataat aatgccgcaa aaactagaac
    19201 cactaaaact gtgatgagtg ttgtcatttt tctcctcctc atattattaa tgacaagtcc
    19261 atcgaaggaa gtaaaggttc ccgagaatga ttcttcaacc atcctcctga aaaaattttt
    19321 ttcagtgact tttatatttt agacgatttt tatcgaaatg acattctttt tgcttacaat
    19381 tagtaataaa aaaacatacc tgtgatagct ttaatctatc gcaggtatgt ttttactgtc
    19441 tattcttgtt tttctgattg aaaattcaat tttaccccag gctgatctaa ggcgatctct
    19501 gtcacctcat aagacattcg gcgtacgata tcgaataacg gtgctactag ctcgtccact
    19561 tcttcttgtg tgacatcatt ttgaccttca attttccgat tgaggatatg attgatctga
    19621 ctcacacttc cagataaaac atatgtatca aacactaaac ggaactctaa gcgtgctcct
    19681 acgatgctat gttctttcgg atagttttcg atcgtttctt ttaaaggcga gaagcctact
    19741 ttcaaatctg tttctacttc ttggttcggc aatctcatat cataatgaaa tgcttctaca
    19801 acttcttttt gtcttctgat ttccatataa ttttatcact cctttatgaa aattataaca
    19861 tatctccttt gaaaggggat actacttttt aatgatgctc tgcaaggtaa ggatttcatt
    19921 cagatttcct accacataat ctgccgggat ctcacccaga aaatgatcga tgtcgtaaag
    19981 acaagtagcg actcctgcat ttttccctgc ttcgatatct aaacgacgat cgccgatcat
    20041 catcgtgtct gttctttcta aatgaaaagt atcgatcaaa taatttaaag atgctggatc
    20101 aggtttacga ggaaagtcct gatcgatccc tactacttcc tcaatcagat gtgccaatcg
    20161 atgttctttt aacaggcccc atgttgattc tgtcaaacgg tgggtcagga tgaaatgacg
    20221 accgccgttg tcttttagca tttccagtac ttgtttcgtt tctggaaatg gacgggaaac
    20281 ttccgtactt tctttttcat atcggtgaaa taattcatta aactctctgg cattcaagtt
    20341 tgattcattg atcaaataac tagtagaata ctttttcatt ttgaagtaga tttctttttt
    20401 gtccatactg ataccaaaat cttttagtgc ttgccatgca ccatctacca ttgcgggata
    20461 cgtatcaaac aatgtaccat caaagtccca tatataattt ttcatgttcc ttttgttcca
    20521 ttcctttgct tcttttttct atctctcact tttagataaa ggtgttcttt aaagtatttt
    20581 tttgtccaga attgtcaaat catctgctaa tgtatactgg attggttgtg catttgggat
    20641 atcgatcgta tccatttgat cttcaggaac atcttctaaa tattttgtca gtgctcggat
    20701 gctattacca tgacccacaa ctagtaaatc ttgccgatct tttagttttg gtgctagatg
    20761 atcttgccat aagggaatga tccgattcac tgtcatttcc agactttctc cgtatgggat
    20821 caaatgcgga tccaagtgat catatctgcg gtcaaatcta ttttctgcgg ttaacggcgg
    20881 aacttccgta tatcctctgc gccatgtttt tacttgatct tttccgtata tttctttcat
    20941 cttatctttg tttttaccca caagatcccc ataatgccgt tcatttagac gccatgtttt
    21001 tattatagga agccataact gacccatttc ttctagtgcg acttgtccgg taagaatcgc
    21061 acgtttcaaa acagaggtat aaacggcatc gaattgaagt ccggcttcaa gcattttttg
    21121 accggcaact gctgcttgtt cttttccttt ttctgttaag gcgacatcta gccatcctgt
    21181 ccagtaattt tcaaaatttg cttcgctttc gccatgacgt aataatacta atttcattct
    21241 tttctccctt aaacattcgt ttcgaatttt ataccacatg ttttgcatgt cacacgtatt
    21301 cgtccttttc ctttaggtgc acgctgcttt tgatgacaat tcgggcactc aaaaaaagta
    21361 taactttttc gatccatttt gacttgcttt ttgtttttta tctgatgata tttttctttg
    21421 actctatcaa gatatttcaa ataacgttga ttctcattta gtctggggta gatttttttt
    21481 gataggaaac gaaatatcat ccatccgaaa aagacgatga acaacaattg tccgattcta
    21541 tagggcaacc aacgacttag caaactaaag acaagcgcag cgatcaatag accgcgattc
    21601 aactgatcaa atttcgcata tcttccctgc ataagtttgt gatagttctg ctgaaatcgt
    21661 attagccttt ctaaccatgt ttgcatcata ttatctcctc catcatctaa tatttattat
    21721 gacaaaaaaa tccaaaaaga aaaagcctta attggccttt tccattgcaa cttggtgaag
    21781 gacattccga tttctggtaa agtcaatcac aatcactttc tttttatctt ctctgttgac
    21841 tactcgaaat gtgtccgtca atggaagaaa cagcttctta gatggtagct gttgcatacg
    21901 tataaaacga tggatcatcg taatcaattg aggaaattga atagacgtgt gaagcagacc
    21961 tggatatttt ttcaacagct gtgtcgtttt gatcagattc cgttttttac tgatcacttt
    22021 taaaaagctt tttagaatct tttgctgacg tctaaaaata gaaaaagaat tttcgtcatc
    22081 tatttgataa cgcatttgtt tttgcagttc tgctgcagtc agatggatct tcccatcgtt
    22141 tgtatcgatc attaaacctt ctggaaacag ttcaaccact gctcgatccc aagattcaag
    22201 agaaagctcg atataatctt ttactggtaa ttccaattct accttaaaaa agtgcaaaac
    22261 agccgacagc ccttctgctt gatagatttc tcctaatgtt agcccctcta gatggaactc
    22321 tggtgaaaaa tgcagcaatg ttttattttc ctgcaattga acatatagaa atgtagatcg
    22381 ctcaatcaga gtatctttta aaacaactaa aaaattcatt tttctccccc ttttttcatc
    22441 atttttaaaa tagcatattt tgaaaagaaa gtctgatgct tagagtagaa tttcataaaa
    22501 atttactatt tcataagtat tcacgagaaa aatacaaaca aaaaagcgga aataacgctc
    22561 atccgccttc ttgtttgtgt ttatttcctc ttcaaacgct tgtctatgat tcagactttt
    22621 ctctatcgtt attttcatct aaaagttctt gcattccgat aatttctcga tattgatgaa
    22681 tcttagtgat accaatgact ttgtctacat caatactaaa acaaatacca ttacctggaa
    22741 tatcgatgtt gatgccttga ccaatcgcat ccattacttt attcgtcaaa ctatcgggta
    22801 ctaatgtcaa cacaatatct ttttctggcg aaatgtccaa acctaaaaat ttccctgaat
    22861 catgtacacc agaacctctt ccatgtaaga tcgttccgcc tgctgcacct gcttcttttg
    22921 aaaagtcgat aacatcttcg cccattcctc cgtcaacgat cgtcacaatc atgtgaagat
    22981 tcaatgctac aagatctttc atttgctttc ccccaatttt tgcaatcttt ctttttcgat
    23041 tctgtctttt tcatcttttc gtttataaat atatcctagt actaaagtgg ataatattgg
    23101 tgtcaatgca atcattgcaa ccacaccaaa cccgtcaatg atcggatctc ttccttcaat
    23161 ttgtgcagcg attgccacgc tcattgataa aataaatgtc gaacacatcg ggcccgttgc
    23221 tactccaccg ttatcaaaag ccattgctaa aaagatcttg tccacttttc gtcctaaaat
    23281 aaatgctagc ccgtatcctg ggatcaagaa ataataaaga gagaatccga cgagcaaacg
    23341 ccacatagac aatcctactg ctgcgccgat cccaattgaa attaccatca gcatgatttt
    23401 cgatcggatt ctcccctcgc tcaattcttc aacctgtttg atcattacat gaatcgccgg
    23461 ctcagcaaac cctactaaga accccattaa aaagccaagg ggaaccagta tataattgta
    23521 aggcaatccc gcaatagcac tgcctaaatg ctgtcctacc ggtacataag ccatattgac
    23581 tccgtgcagg aaaaggatca acccaatagt cgttatcaag aaacctttca taatgtcaaa
    23641 gaaacgcttt ttgcgcatac gaaaactaat gatattcatt aaaacaaata ggattacgat
    23701 aggtgccact gccaaaatga cttccatggt aacgatttcg attccatcta tcacttgctg
    23761 ccagaaactc attggaagat cacccccaaa atcatcacgg caagaatcgg ccctaacgaa
    23821 gcaataccga ccattccaaa actgtcattt tcatttttct tttgtttgat catactagtc
    23881 atccctccgg caagggccaa gataaatgga acagttactg gaccagtagt cactcccccc
    23941 gaatcaaaag caatcggcat aaattcggaa ctggtgaaaa atgaggcgat caaaacgcct
    24001 atgtagccta tagccatcaa ttgataatag gatagcttga atacgacccg aagcagcgca
    24061 aaggctagaa atactccagt gccgacgctg acgatcccaa tcagcaacac tctgccaatc
    24121 tctccgtcag atatatcata tacttgttgt cccaaaactt gcacggaagg ttcagcaatc
    24181 gtgatcacga tcccgatagc aaatcccaaa ctgaccaaaa cggtcaaact tttctttttg
    24241 atcatatatt tccctactag atctccgact tccatcatag aataatctgc tccaaacaaa
    24301 aagagcgtca ttccgatcat catgattgcc gcaccaacca gaaatgaagc gaggtcttct
    24361 ccttctaacg gtgcaaaaac aaacgttaaa atgacaatca ggacagtcat cggcaatata
    24421 gcagcaatca cttctttaaa attttttagc atgtatcctc ctcctttact ctttaagttt
    24481 accttaaaat gaaagagttt tcgagcacaa acttaaaacg ttatcattta atttttctca
    24541 acataaaata acacacgtcc caaaagtatc tttttacttg gaaaaatgac tttgaaggaa
    24601 cgcgcttatt gtgttttttt attttacaga gccgatcatt ccttgaacga attgcttttg
    24661 caaaacaaaa aatacgatgg cagttggtaa agtagcgatg actaatccag ccatgatcat
    24721 tccatagtct ggtgtatagg aagcacccat agcagataaa acaagcggaa ccgtccgttt
    24781 atcaggagat tgcaaagcta ctaagggcca taaataattg ttccagctac tcatgaatgt
    24841 gatgattgca gccgcggcat aggtattttt agccgtcggc atatacactc taaaaaaaat
    24901 acttagttct tttagaccgt ctaatcgagc ggcttctact agatcttttg gaaaagattt
    24961 aacattttgt cgaaagaaga agatcaaaaa agcagtgcta acagatggca ggatgacaac
    25021 caagaaactg ttgattccca aaaccgttcc gttcaattgt gaaaacattc gatataaagg
    25081 gatcatcaat gccgcaaaag gaaccatcat cgatagcagt agaatattga aaattaggtc
    25141 ttttcgttta ctttgataaa tctcaaatcc atatcctgcc attgacgaaa tcagcaatgc
    25201 aaacacagtg gtcaccaaag cgatcatcgc tgaattccaa agggaacgag taaaattcag
    25261 ctcattggaa aacaaatttt gaaaattgat gatcaagtta ctgccaatct ttatttttcc
    25321 cgatgtgata tccacgggtg tattcgtcat tcccaaaacc atccagacaa aagggaaaat
    25381 ggaaataaat gataggatgc ttaatgctgt atactttaat actgacccaa ttgtcgaacg
    25441 aagttctaat gcttgttttt tgacgttggg gcgttcgatt gtttcttttt ctaccatatt
    25501 tttcatcgac tttctcctcc tactttcgtt tggattatcg acaatatcac aataaaggaa
    25561 acaataacaa aggaaactgc tgccgcatat ccaaaattag gcgtgaattt ataactcaaa
    25621 ttatagatgt attgagatag ggtggtcgtt gcatttgctg gtcccccgcc agtaatattt
    25681 tgtacttcat caaaaagctg caatgtcccg atcgtcgaag taatggaagt aaacaaaatg
    25741 attggtttta aatttgggat cgtcactttg ataaactgct gccatttaga agcaccgtca
    25801 atagatgctg cttcataaat ctccgaatca atattttgca tgcctgaaag gaaaaaaatc
    25861 atattgtatc cagtccaacg ccaagtgatc gaaagaatga tcagaactct cgcccaaatt
    25921 ggatgagtca accagccaat cggtgtgtca atcataccaa tgcccaacaa caagttgttt
    25981 acaaggccag attgagaaaa tagacttttc atgatcagag aataactgac catcgaagtg
    26041 atacatggaa gaaagatagc ggttcgaaac aaccctttat atttcaactt tttatcattt
    26101 aaaaggttgg aaataaaaag tgctagaaac aacatgatcg gtacttgtac aagtaaatac
    26161 aggaatgtat taaaaaaagc ttttttaaaa gttgcatctg tcaacatccg ctgataatta
    26221 cctaacccgt taaatgtcat attgacaggg ggaccacttt gaaaagacgt gatcaaagcc
    26281 aaaaccatcg gaataaaaac catgattgat atcaaaatga ctggcaagat taaaaaagca
    26341 tgcccgtttt tgaataaatc tttctgcttc attcttttcc ctccaattct atcattgatt
    26401 atctacatga aaagacaaga aagacaatat ctgtaaggca gacgtgatct tttttgtctt
    26461 ttcgtttgtc tgatgaaaaa gcgaagaaat tcactttttt accaccgatc aattagccaa
    26521 ttgtgcttct acttgttttt gtgtatctgc tagtaccttg tctgtctctt ctccattgat
    26581 gattcgatga agagcttcag ctacgactgc ttcaattgca taggtctcat gtccgtaatt
    26641 cacttctggt atttcagatg tccatgttga aaaatcgtca aagatctttt gaccactgta
    26701 aaattcagaa gcttcttgat aagcttcttg ttcattcgcg ctaagcatcg tagatactag
    26761 tccgatttct ttcgcaagag tgcccatcag ttcttcattc gaagcaaacg ttgcttctag
    26821 gaaatctttc gctgaatctt ctccagctac tcctttgatc acataccagc cagcgccgcc
    26881 aaggttagaa gcttgcgctt tttgcagatt ttctggaagt gcaggtattg gtgcaatctt
    26941 ccattttcca gattggtttt ctgctccttg gatcgttgaa gaataccaag cgccagttgg
    27001 gctagaagct actgctcctg attgtatcgc agttactcca gcattccagt cagaggtttg
    27061 ttcgactaga tcttctttca aaagagtggc aaacaattca agaccatact ttaaagatgc
    27121 attgtcttgg atcgtgactg tctccccatc ttcagatgta taccattctc ctgcctgttg
    27181 cataatcatc cttactcgac caaggtcaga aggattgact tcagcgattt tctttcccgt
    27241 cttttctttg acatctcgcg ctacttgaat atactcgtcc cacttcagat tctccatatc
    27301 ctcttctgta taacctgctt ctgccatcag atccgtacga tagaagtttg ctgttacgcc
    27361 cgaatcgaaa ggaacaccgt atattttatc tcctactttg tttaccgcaa atttatacgg
    27421 tgcaaaatga tcttccttta caatagcaga caaattagcg aatgcatctg gataagaagt
    27481 caaatagcct tgtatcctat aatcttcaat caagacgata ttgggcaatc cttctgtatt
    27541 cccgctcgct aaagcggtgt tcagtttctg aacgatatca tcctgagaca tcgtaacaac
    27601 ttctatctct gtttcttcgt tctcataaac tttttttgct tcattcactg ctttgatatt
    27661 gaatgtctca tcccacgccc agacagtgac tttttcttct ttgtctgctg tactactagc
    27721 acttccgcat cctgttaaga aacctgatgc tgccgctaaa caaacgactc cagcaattaa
    27781 ctttctgatg ttcattttta tccgctcctt ttccatccat tagttgttta cgtttgctac
    27841 attcatcata ccaaaatttg aaaacggttt ctttattatt cagatacttc aaaaatttct
    27901 cctaaaataa taaaaaaata caaatcaact atgacaaaag gaataatctt agcatctaga
    27961 ccaaagatcg aaatggtgag gtttctttaa aataaaaata tccttgcatt ttctcctaga
    28021 aataagtatt tttaaattac aagactattc ttaaggcggg taaaaaaatg ccaatacgcg
    28081 taattcttat cgatgatgaa cagacgattt tagatggaat gaaacagttg atcaattggt
    28141 ctgagcacgg gtttcaatta atagggactt tttccagtcc gctgaaagcg cttgattttt
    28201 ctttggtaca tgcaatcgat atcgtggtca cagatgtaat gatgcctgaa ttaaatggaa
    28261 tcgaattatc gcggctatta aaacagcaac aacctgaaac acagatcctt atcctctcta
    28321 gctatgatga atttgatatg gttaaagatt cttttaaaga aggcgtcgct gattatcttt
    28381 taaagcctcg attgagtcct gatttttttc taaattcctt aactgccgtt tcacaaaaaa
    28441 tcaaaaaaaa gcccaacaaa agcccaatca ccaatcgtta tgagcagatc tgcgagaatc
    28501 tttcacagca gattgctgga caaactcgtc cattgcttga ccctcttctt tttgaacaca
    28561 ccttgttttt ctgttatata ccgaaaaacg gccattcagt tcaaaacaag gctctaaaca
    28621 aaaacagtcg atcacccatt cattgatgga tagcgacgat tcgtttacta tttatcctgt
    28681 ttctaccttt tcggaagaat ccggctatgt aatcaatact gattccaaag atacacttag
    28741 gaagtttgtt caaaagtttc gtcaagaagt ttttccaggt gatttttttg ttgtttctga
    28801 ttcttttgca atccaagata tcaatgaaat attccaagag atcagaaatt gcaccaaagg
    28861 acaacgtttc tatcataagg gaaagaacct tgcttatcag catgaacttc ttccgttatt
    28921 aaccaaccac gagcagcaag caagtagtta tttaacaaaa atcgttcata aagactacgc
    28981 tttagctgtc aaagaacttg agaatttctt cacgacgata ttggaacagc ctattgatcc
    29041 agcttatttg aaacatgagg caatcaatcg tttctatgca ttactcaacg ctttggctga
    29101 cgagcatgtt gggaatgaag aactttcttt gctcaagatc gcccttcctt ctttattagc
    29161 cgatgctgag tatttcgaag atttttctat tcttgtcaaa gaaacacttg agaaactcac
    29221 ccacttttct gcaaatgcaa aaaaagacaa tttcgatatc ttgactttga tctacgagtt
    29281 cgtccaggaa aattatgaga aagaaatcaa tttacaactt ttatccgaaa aatatcattt
    29341 ttcatatagc tatctctcag ccatctttac tgaaaaattt ggtatcaact tttcaaaata
    29401 tctaaaaaaa gtccgtatca gtaaagccaa gctctttttg acggaaacac ggcttacttt
    29461 gacggaaatc tgtgagaaag tcggctatac cgaactgggt tatttctcca gagtttttaa
    29521 agaagagaca ggaatgacac cttctcaata ccgtaaagga acttttgtaa aatgagaaaa
    29581 aaaatcaaag agcatgaact ctttgccaaa cttctgttca ttttgttcat cggtcttttt
    29641 ctgcaagcca tcgtcatttc tttgtttatt tatcatcgat caagggatgc ctatatccag
    29701 ctgttcaatc aatcgaatga tgtggtacta aaaaagattc aatctgaatt tgaaagtttg
    29761 aatgatacaa tcgagaatac attagccgct tttgattcta atccagctgt caaaagctat
    29821 ttttccaatg atccagccca acatatggaa cagcttcagc aactaagaac aatccaaaaa
    29881 atgaatgact ctctttcaaa aatccatcca atgatcgatt acgatgtatt gatctttgga
    29941 gaaaatggaa gaacttttgt cggaaacgac atgctcactg ccgtatctgc cgattctttt
    30001 tttcaatctg cgattgctca gagagtgaat gaacgtgctg ctgatacaca aatgcttttt
    30061 gcacaccacg gattgactct tcgcgataag aaagcaccca gcgtcttctt tgtcaaaaaa
    30121 ctgaaaaata cattgaatca cgtttacgga tatgctgttt tatcgatctc atccaagcaa
    30181 ctagcgaatc tttttcaatc tgtagtaaat cctgagatat cgaagatctc cttcatgaac
    30241 gaagaacaga cgatcatcgc atcaaatgaa gaaaatacaa ttgggagccg atcaatgatt
    30301 ttcaaaaaac taacagtagg tgaaacaacg atttcaaatg gacagcagtt gacccgtctt
    30361 cctctgtacc gccaggattg tacattactc agtcaagtcg atcttcattc tttatctaac
    30421 caaatgggcg tgatttttcc gattattttg tataatatcg tgacacttgc ttttgctggc
    30481 tcattggtat ttatgtattt ggatcgacac acaaaatcga tctaccgctt gattgattcg
    30541 ctaaaacggc tagaggaaga tggcttaaaa gctactgttc ctgtacaagg aagccatgaa
    30601 gtccaattgc tagggaatac gatcaatcaa ttgctaagta atgctcagca tcattatgaa
    30661 cagacgatcc aagacgaaaa gaagaaacgt tcgcttgaaa tccaaactat gcaagcacag
    30721 atacagcccc attttattta taatacgctg actagcttga aatttttgat ttggcagaaa
    30781 aaaacagatg aagcagttca aggaatcgac agtttcatta ctttactaag aagtacgatt
    30841 gggaaaaaag aagaagtgat tccagtcaag gaagaaatca aaagcgtaca aagttacatc
    30901 gatcttttga gtatgcgata tggcgaaaga atctcggcta aaataatgat tcctgatgaa
    30961 ctgcagtcat tatctatgcc gaatatgatc cttcaaccaa ttatcgaaaa cgcctatctt
    31021 catgcttttc acaataaaac gcaaggattc atcacggtgt acggcaaaac aaaaaatcag
    31081 cttcttttat tcgaagtgat tgataacggc gatggcttcg atactgaaaa taccaaacaa
    31141 aaaaatgatc atttttcagg aatcggcatc agccatgtag atgagcggat acgcctaatg
    31201 tatggcgatg catatggctt aacgatccaa tcagtgattg ggacaggaac gactgtaacg
    31261 atttgtctac cgcttcttcc taaaaatcaa tcagaatatc aaaaataaag aatagccaag
    31321 cgctgatttt tttattttaa gttaaataaa tcaatcagta ttcttgactt tcatctacgc
    31381 gtctcttcta ctgtcagcac tggataacag cccaatattt gactttctcc tccgagcatc
    31441 ttgatttctt ggagcgcatt ctctactaat gtcattggac ggtccaacaa caaatcgatc
    31501 acgaaaaaat attctcccaa gctagttttc aatggtcggg attcaatctt acttaagttg
    31561 atttcccgcc agccaaaagc tgccagcatt ttatgaagca tccctggccg attagcaggt
    31621 agtgtcaaga tgacactcat ttttactgga gctgactgct ggctggtcat ctttcggtca
    31681 ccgacgatcc aaaaacgtgt ctggttcaac tcattatctt ggatattctc agctaagatc
    31741 tccaatccaa catgctgtgc tgtttctaat gaagcaatcg ctgctgcgtc ttctttcgga
    31801 tgttctgcta catacattgc tgcagcagtt gttgattctg ttgctactaa aggaacatta
    31861 ggataatgcg tttctaaaaa ctgttgtgat tgtgctagtg cttgtggatg tgaaaggatt
    31921 tttgtaatct ttgttgctgg attgcctaaa agctgctgct tgataggaag aacgatttca
    31981 cttttaactt cgacttctgg atgtttggaa agaagatcga ttgtgtgatg aacagctcct
    32041 tccaatgaat tctctacagg aacgacagcc agatcgactt gtttgcgaaa caaagcgcgc
    32101 aaacaaacag ggatggaagc atacgcagtc aattgttctg taggaaatag ctgacaagcc
    32161 gcctgataag tgaatgaact ttctggccct agatagctga cgttcatatg cttttgatcc
    32221 tttctataat ggtatcgatg atcttttctg gtgtttgatt ggtcgtgttg attcgatgtg
    32281 tcgcactttc ttcataccaa gaaagtcgtt gtgccaataa tgcttttatt tcttctcttg
    32341 tttttccaag agctaaaggt cgaatagacg tttcatcttg ttggatacga tcaactagac
    32401 agtccgcttc tgcctctaag taaagaacaa tcggctgtgt ttttaaaaaa cgccgatttt
    32461 ctgcactttg gacaatccct ccaccagtcg cgataatttt gtcttcattt gacagctttc
    32521 ttaaaatatc tgactccact tttcgaaagc ttttttcacc gtaatagtga aaatattcgg
    32581 caatcgacaa ttggatatgt tcctcaatct ttgtatctaa atctacatac tctttttgta
    32641 aacattgagc taacccttta ccgattgttg tttttccagc gcccatgaaa ccaatcagca
    32701 aaatgctagc catgagcttc tcctcctgac acaagttctg ctaaatcgtc aaaaaatgcc
    32761 ggataagaga ttgaaacagc ttcagctcct tctagttcgc aaggtccatc agctatcaaa
    32821 gcagctactt gcagcatcat accgattcga tgatcgcctc tgctggacac ttttgcgcca
    32881 tgcagaggtg tcggcccatg gatgatcaga ccgtctgctg tcgcttttat ctttgctccc
    32941 attttctgta attcttctgc caccgcatca attctgttgg tttcttttac ttttaattct
    33001 tctgcgtctt tgatgatcgt gattccttct gcttgagtag ccactaaagc tagaatgggc
    33061 aattcatcaa tcagacgcgg aatgatttct ccaccaatcg ttgctttctt caaatgactc
    33121 gtcttgacac ttaaatcagc cgattggttc tgttcattgt gattcgtctg ggtaatcgct
    33181 gcgcccatct cctccaacac atctaagata ccagttcttg ttggattgac cccgacattt
    33241 tttaacaaca gttggctttc cggaacaaga agtcctgctg ctagaaagaa agcagctgat
    33301 gaaatatcac ctggaacagt tatctgttga ccagttaatt tttgcggtcc ggtcactatg
    33361 atcgttttat cttccactgt gatccttccg ccaaattgac gaatcatttc ttccgtatgg
    33421 ttacgggaac gttctttttc aacgattacg gatgtgcctt ctgcttgtaa tgccgcaaac
    33481 agcaaagcag atttgacttg cgcactggct acaggcatat tgtatcggat aggtgataaa
    33541 gagcgggttc ctgtcacagt gattggtaga tactggtcat tttcttctcc ttgcaagtca
    33601 gcgcccatct cgcgtaaagg ttccatcact cgccccattg gacgtttaga caacgaagca
    33661 tccccgaaca gagtagtcgt aaaaggctgt ccagccaaaa tacccatcaa taaacgtatg
    33721 gtcgtaccgg agtttcccat gtcaattgat tcggttggtt ggacaaatga gtggctaccg
    33781 cgaccatgaa tcttgatcac ctcttcctct tcttcgatct cgacacctaa ttgttggaag
    33841 gcatgtaaag tactcagaca atcttctgcg cgcaaaaaat tttgtatagt cgtcgttcct
    33901 tcagcaattg ctccaaacat gatgctgcga tgggaaattg atttatctgc tggtatccta
    33961 acagtccctc ttaatccatg tatttgttgt aataattgca tttatccagc ctcctatttg
    34021 atccagcaat gataactggt attagacata atgattcttt ttgcttgtaa caaatcctgt
    34081 tggtttttaa aactgatttg cagtattcct aaaatatctt ccctggtctc ttggatcttc
    34141 aaattaatca aagaaactcc cgcttttccg atcagtccag ttacttctgc aataacgcca
    34201 ggttcatctg gcacatcgat aaataaatca aaaaaggcag gaatcgcgcc ttgcttatgg
    34261 acaggcaact ggtctcgact ttcttttgcc cgattgaaaa aagaaaagat ttccgattgg
    34321 ttctcagtca tgatccattc tgctatttgt gtcatttctt gttgccaatc tgccaatcgt
    34381 tccaataata tctgcttatt gctcaacaaa atatctgtcc acatgacagg atctgaggaa
    34441 gcgatcctag tgatatctcg aaagcctcct gctgccagtt gtttggctcg cggatactct
    34501 tgattgaatt gttctgcctg attgactaag ccagctgcca gaatatgtgg aaaatgactt
    34561 agcattcctg ttatacggtc atgttctctt gctgtaagag ttacatattt tgctttggtt
    34621 ccgcaaaata gttcttgcag tttttcaaca tcttttttcg tcttttccaa aggtgtaaag
    34681 atgtaatagg cattttcaaa tagtttagca tctgctgcct gtatacctga tttatgagaa
    34741 ccagccattg gatgtccacc gacaaaccga agatttttct gctcagcgga tgtcacgact
    34801 tgctgcttcg tactgctgac atccgtaatg atgacgtgat cagacaagtt gattctttca
    34861 aggacatcaa gataagctaa ggttgtacgg acgggaacgg ctaataagat aacgtctgct
    34921 tgttctgcac ctgtttcaaa atcacttgca acgtaatcaa cgatctgctg ttctactgct
    34981 tgctcacggg tttttgactg actatcccaa ccaactacta tcagttctgg attggtctga
    35041 cgaatacaaa gtgccataga agcaccaatc agtccaagcc ccacgataaa aacatgttgt
    35101 gccatcttat ttgccccctt tttagtaatg tttcaagtag tcacggtact ccttcactgc
    35161 agctttcaac tcaacaagcg aatcacttgg gaattttgac aatagctctg tcgctaccac
    35221 agtggctaca acattttccg caacgacgct agctgcagga actgctgtac tatccgaacg
    35281 ttctacactt gctttgtaag gttcttttgt atcgatatca acactttgaa gtggcttgta
    35341 caatgtagga atgggtttca tgaccccgcg aatgacaagt ggttcgccat tggtcattcc
    35401 gccttcgaag ccgcctagat tattggtacg tcggaaatag cctctttctt cttcccatag
    35461 gatctcatcc atcacttctg agcctggtaa tgaaccagaa gtgaatccga ccccaaattc
    35521 tacgccttta aaggcattga tactaaccac agcttgtgcg attttggcat ctagcttcgt
    35581 gtcccattgt acgtagcttc ctaatccagc tggaacgcct ccaacgagca cttctacaat
    35641 tcctccgatc gtatctccat ttttctttgt ctgatcgatc aaatctttta ttctttgctc
    35701 aactgctgga tcaacaactc gtacttctga cgcttcagaa atttctttga tttcttgaat
    35761 cgagatattt ccaggtattt ctgcttggat tccaccaaga caaatcacgt gtcctgctac
    35821 gatgatgtca agttcttcca gaattttctt ggcaattgcg ccgattgcta cacgcatggt
    35881 cgtctctctt gcagaggacc gttcaagcac attccgaaga tctcgatgat ggtatttcat
    35941 accgcctacc aaatccgcgt gtcctggacg cggacgtgca actcgccgca gtgattggtc
    36001 tttttcaggc acttctgcta cagacatgac agttgtccaa tttttccaat ctttattttc
    36061 taccactaaa gtgacaggtg aaccaattgt tttgccgtga cggatgccag acgtgatccg
    36121 aacacgatct ttttcgatct tcatccttcc tcctctgccg taaccagctt gtctgcgagc
    36181 gagttcgtga ttgatttctt cttctgacag aggtaatcct gctggtagtc cttcaataat
    36241 ggctgttaat tctggtccat gggattctcc cgcagtcata taacgcatct tacagtcctc
    36301 ctaagtaatc tttgaatgtc tctgtgggaa caggaacgat ttttgcttgc ccaatctttt
    36361 ctagcaagat gatattcaat tggcttcctc ttgctttctt atcatgggtt atcgcgtgga
    36421 ataattcatc tggatgactt agatcgtacg taatcggcag atggaatttt tgaagtatcc
    36481 tttctaaagc agctgtcgat ccagttggag acaagccgat tttttctgca tgagaagtga
    36541 tggctaccat tccaatagct actccttctc catgactgac taccccatat cctgctgtat
    36601 tttcaattgc atggccaatt gtatgaccaa aatttaaaat aagtcgattt ccttggtcaa
    36661 agacatcttc ttccactact tttcgtttga tttccaacgt agcagtaatg attggaacag
    36721 catgttccag aagatctttt tcgtctttca gctcgttcag ctgtttccaa agcgttgcat
    36781 ctgctatagc tgctgatttg atgatttcag cgattccttc tcgtaaacga cgtaccggca
    36841 aagtttttag aacctcagga tcaatcaata caccagctgg ctggctaaat gttccgacaa
    36901 ggtttttcgc tttgtttgta ttgactgctg tcttcccgcc aatactagaa tctacttgtg
    36961 caagtagcgt agtcggaatt tgaacgaaat ggatcccacg catgaacgtc gaggccacga
    37021 atgctgctag atcacccacc attcctccac caagtgcaag tatcccatcg ctgcgggtaa
    37081 aatcattatc tgctaaaaaa tcgtataact cagctgcttg ttctaaagat ttactggtct
    37141 cacccgccgg tacgattgaa aggactggtt caaaattagc ttgtctcaat tggaacatga
    37201 ttttttctgc atatagtgga gcaacgtttg tatcagtgat cacagctact ttttgaggtc
    37261 tccaaatcgt ttgaagccat gatccagcct gttctagtgc gcctttttca atatgaatat
    37321 cgtatttttg gttatcaaca tgtacagtaa gcattcgttt cacttctttc ttttaaaaag
    37381 ctggaattaa tcgagtgtct gaatcttttc cattgcttct ttcatgatcg caacttcttt
    37441 catcatgcgt gagtaagtct tttcattcaa cgattgggga ccgtctgacc aagcatttgc
    37501 aggatcagga tggatctcta cgatcatgcc atctgctccg cttgccacac ccgcacgagc
    37561 cattggtggt acaagttccc aaattcctgt accatgactt ggatcaacaa tcacagggaa
    37621 atgagttaat tttttcatca atgggacagc acttaaatca aacgtattac gtgtcgctgt
    37681 ttcgtaagtc cgaatcccac gttcgataaa aatgacagga gatttgtctt cgactgcaat
    37741 gtattctgca gcattaagcc attcattgat cgtaccggaa attccacgtt tcaatccaac
    37801 aggtttacct gttttaccga cagcagatag cagtttaaaa ttttgcatat tacgtgcgcc
    37861 aatctgtagg atatcgctgt actcagccac catatcgata tgcccttcat ccatcacttc
    37921 agtaatcact ttcattccaa attcatcagc cgcttgacgg atgtatttca atccttcttc
    37981 ttctaaacct tggaaagcat atggagaagt acgtggtttg aaggcaccac ctcgcagaat
    38041 ttttgcgccc ccggctttgg ccattcgcgc acattctctg atttggtcta atccttcgat
    38101 agaacaagga ccagccatcg tcacgaaact gccgtcacca atttttactc cgtctacatc
    38161 cacgactgta ttgccaggat ggaattctcg agaagtcaat ttgtatgtgt ttaaaatacg
    38221 cacagcattt tctactcctt catagcgtgt aaatgctcct tcttgtaaac ttcgcgtatc
    38281 accttttagt ccaatcacta ctcgttctgt tccttgatcg attgagactt tgaatccttc
    38341 tttttcgata tgatcgacga ctttttttat ttgttcttcc gttgcttctt ttttcataat
    38401 tactatcatt gtttttcccg ctttcttttg tttacttatc tatcttggct gattttctct
    38461 actttttcaa ggtggtttta cattaattcc tactaaatga ccgtgtcctg ttgttctcat
    38521 tttcgctcct tctttcctac ttatgaccca gataatcaac aaaaaaagat gtccctgtag
    38581 gaaattgcct ctacagtgaa catcttcaac gagccttaaa aagcgctgca aaaatataaa
    38641 atagccacta tagatgaatt tctctacgtg gctgtaagtc gtgtttaaaa atctttagcc
    38701 atcatagata caaacctggt ttgtttgtac ccacgaagtc atgaatacaa acctatctaa
    38761 aatagctaaa gtaatacgta ttcagttttg taagttttct agatttcata aaaaatcgct
    38821 ccaaactgat gtaagaattg tacctagtat agacataaaa aaatcgactg tcaatcattt
    38881 ttatgaaact ttttttaatt ttctgatagt taactttgtt tttcagaaaa ataacaattg
    38941 gttttattga agaaaatagt catgaacaaa agtatctgag aactttttta ctctcttgtt
    39001 catgacatat agaataaagc agctttgtct tgtaaactcc gtttttcaaa taataatcta
    39061 aagattgcta ctgaataata aagccgattt catggtcctc ttcgctgctg attcggtaag
    39121 gtgactcaac atccgctccc cagctattga tacccccgac tcctcttact tttgcaaaaa
    39181 tcgtaagaac cgttcgtctt tctggcggta gttcttctat atgtgttgcc tgttccagtt
    39241 cttctgcttt gaatggtaaa gcggaaaaag caaatggcgt atcctgctgt tcaaatcgca
    39301 acgaaaaatc ttcttgatct ttatccgtat tatccaacgt ttgatttctt gttagttcta
    39361 cccatttcgt ttccatatgc atgccgcatt cttggggaac aaggtatggg gaaacgggga
    39421 gtcctgccac tttataaata ccttcgacac caccagccat ccgatccgga tatgtttccc
    39481 ctgataggcc ttcatactca aaaaattttg ctttcgttgg cataatgaac cttaaaccca
    39541 atgcgggtaa ttccggtaat cctttttgtc cataataatg catattgaca agcattttac
    39601 cctctccagc gatttggtag gaaattgtca cttgggtttt tggtactgtg ttgatttcca
    39661 gtgtaaaaat gatttcaacc gtttcagcgt attcattact tgaatattga ttatttactg
    39721 gagcaatcgg caaatcgatc tttttattgt ccaccagtac ttctatttct ttgacttttg
    39781 gatacaatcc ggctcctaac cacataccgg atttaaatcc aaatccgcta cctctgtcat
    39841 tatcggtcgt tgctcgccaa aaaactggaa gtgtttcccg gtatagccat tcttttccgt
    39901 ttttattgag cgattccagt ccgcctcttg tataagaaaa aatataatgc gtattccccg
    39961 atttgacacc taatgtacag tcgccgaaga cgattgcaac tttttccgta tccatatgac
    40021 ttttatttac tgtatttttc ataataatat cttacctcct gcatggcggg tttttcttgt
    40081 ccatctgcaa aaacgatccc attccctgag aattcatagt cacttggttt atcgtcaaag
    40141 tcaccgccat agcgcatcac ttctctgcct gtgattggat cagtcacttg gatcgcctga
    40201 tctttaaaat cccatatata gcctccttgg tacatttcat aacgatcaag caacgtcatg
    40261 taagaatcca ttccacctaa cgaattcccc atgtcatgca tatattcaca taagatgaac
    40321 ggtttttccg ctttattttc aaggtagtca atgatctctt ctggtggcgc atacatgcag
    40381 ctttttacat ctgaaatcga atcttcgaat gctggatttg gaaaattccc ttcataatgg
    40441 acaagtcgat cggggtctac ttttttgaag taatcgttca tttgtcgaat gttttcttcc
    40501 gcataagatt catttccaag agaccaaaaa agaatacttg gatggttttt taatgtttcg
    40561 aaattcgttt ttgctcgatc aagaacagcc gcctgccact gaggtactgc acctggtacg
    40621 ttgtaagaag gctcaaatgc gcccattttt tgccatgagc catgggattc gaggttcgtt
    40681 tccgccatca catggattcc tgcttggtcg cataaataat accaagtgat ctggttgggg
    40741 taatgagatg tccgtacgcc gttgatcttt gcatttttta tgatagccaa atctttttcc
    40801 atatcttcta tagtgatggc gcgtcctttt ttcggatgcc actcatgtcg atttaccccg
    40861 ttgataatca tccgttttcc attgaagtag atgattttat ctttgatttc gattttacga
    40921 aaaccgaagg aatagtcaat atactccgtt actctttttt cttcgtctat cgtctccaat
    40981 tcaagcttat ataaattggg ctgaaaatgc ccccacagtg ccacattttc aaatccatcg
    41041 atgcgaatcg tcatttgctc ttttaacgga tattgctgtt gatagatttc cttcccatca
    41101 ggatctagta tcgctacatt cagctgactg tttttggcta cttgctcaaa agacaaatct
    41161 acttcaagtt tgccattttg acatttgtca tctaagcgag gtttgatcca aagatcttct
    41221 aaatggtttt gtcctcgagc aactaagaag acatctctga agatcccaaa aaatcggaaa
    41281 aagtcctgat cttccaagaa tgcagctgta cttcttttgt aaacctccac agctagaata
    41341 ttttcttctt ccaaataagg tgttaaatca aattcagacg gagtgaacga atcttcggca
    41401 tacccgataa aatgaccatt caaccaaata aacattgctt gttctacacc agcaaaatga
    41461 atcgagattt gttttccctt tagaccttct tctaatcgaa aagtcgtcaa atacgaacca
    41521 accggattgt ctttagcagc tgaaaagatc ccttccgcct ctttctcagg gcttgtcgtg
    41581 aaagggggac ggcgaaatac atgtccctcc cacggataca tcgtattgat gtaatggatc
    41641 ttgtcataat ttgctaattc aatatgacta ggaacagtga tttcatcaaa agaacaggca
    41701 tcaaaatctt ttttataaaa tgtttttggt cgtaccgaag gatttggcga ataacaaaaa
    41761 cgccatgatc catttaaaga ctgtttaagg gaggatgtgg tttctacttc ttcccttgtt
    41821 gcataacaaa catgatcgct atgtgcattt gattggttca ctctaaaaac ttgtggatca
    41881 tctaaccatt ttatatttgc tttcatactt ttttccttct ctctcataac tttttgaaat
    41941 gaagcagttc tgtttggaaa tcaccttgaa aatcggggaa gtaaagtcca actgtcatca
    42001 attcggcccc cgaaaatatc cgcttcgagg tctgttcctg ataaagtgcc tcatcatcta
    42061 accccatcag atatacttca tggaacgctg gttgagctga agctagtatc ctccctaaaa
    42121 acacaatagc ttcatcttgt ttaggagaca caaacatcca tgcggcaata ttttcttcaa
    42181 aaggactcgc caaacgataa taatggccat actggatcac cggacgaatc gtttgatagg
    42241 atataatttg gtcttttact tgcttttttt cttcaggaga cagctttgtc aaatccagtt
    42301 cataaccaaa aacagcgctc atagctgttt ctcctcttgt tttcaacgaa gtagatctgt
    42361 cggtctgatg atttggtaca gctgatacat gagctgtgat ggacgaaatc ggataagcaa
    42421 ggcttgttcc atattggatt ttcatacgct ccactgcatc cgtgttatca cttgtccatg
    42481 actgcggcat ataataaata aacccggcat caaaccgtcc acctccacca gaacagcctt
    42541 cccataagat ttccggataa tcagttgtca aatgttcaag tagttcataa agcccgagca
    42601 tataacgatg cgctacttct ccttgtctct gtgggtcaag ggtaatcgaa tagacatctg
    42661 aaagcgaacg gttcatatcc catttgatgt agtcaagagg aatcgtatct aaaatctttc
    42721 tcatttgttt ctcaatcgtc tgccgaacat ctaaccttgt aaaatccagt acatgttgtg
    42781 atctggaagc agaaggcatt ctacctggct cctgcataag aaaatcagga tgtgttcgat
    42841 acagttcaga atcaaccgag atcatttcag gttcaaacca taaaccaaat ttcaatcctt
    42901 tgctatgagc ataatctgct atttcacgta atccatttgt caacttgcct tcatactcga
    42961 accagtctcc taatgaagaa ttgtccgcat ctctttttcc aaaccagcca tcatctaata
    43021 cgaacatttc gatgcctagc tcctttgctt catcaatgat cgccttgatt ttttcactat
    43081 tgaagtcaaa gtaagtggct tcccagttat tgaccagaat cggccgttca gcaaattgat
    43141 gacgacctct tgcgattctt tcacgtagca actcatggtg gatctgactc attttgttca
    43201 atccctgact tgagtaagaa aggatcattt caggtgtttg aaaagactgg cctgcattca
    43261 attcccacga aaaacgatgg ctgttgatcc caccgacgac tctcaattga ttgatctgat
    43321 ctttttcaag ttcgaaagaa tggttgccgg aataaacaaa ttgaagtccg atcgcttctc
    43381 cagtattttc tgtcgtatga tgatgaacta aagcaataaa gttattcata tggtgactgc
    43441 tcgttcctcg acggctttca aatacttttg ttccagataa aacagattgc cgtgaaatct
    43501 gtctttcatt gacatgtgct cctggcaaag caatgacttc atcaaatcgc ctagtatggg
    43561 caaagtctag ctggaaacta gccgcttttt ctagtttgat tgtttctccg gtttcatttc
    43621 ttatctttac agatcgtgtg atcactgctc tatcagtaaa tatggtatag aacaaatcga
    43681 aataaatcgc tgtttctcta tccttaagtg aaatcaccaa cgtttgtgcc tctgacttgt
    43741 ccatcacata agtttgaggc aagccttcga tctctggctt cccatctgta atataatggc
    43801 tgtcatatct cagatccagt aagtctgatc cattcttgcg ctgaatgatc gatgcgggcg
    43861 ttcgaaagtc catgctgcca tgagaagagt attcttgcgg tagtgtatct ttcgacagag
    43921 accggtcagg atttaaggga acatttcctg aaaatccacg atctcttcgg ggataatttt
    43981 tattgtcttt gtactgcttg actggtttgc caaaataaat atgcgccaat acattgcttt
    44041 cttctatctt tatcagataa gaaagaaatg tatttcgtaa atggaagact cggctctttt
    44101 catcaaataa gatgaaattt ttatctgttt ttctatgcgt aatatccatc atttcactcc
    44161 caacaaaacg gaaaccgttt actttttttt ataattcctg tatactgttt actaaacaat
    44221 tttgtcaata aaaatttttt atttttttga ggagtgtgta aaaatggttg gaattagaga
    44281 tgtcgcacga aaggcaaatg tctctcctgc tacagtttcc cgtgtgttga ataacgatca
    44341 gacgatccat gtggctcaga ctactcggca acggatagta gaggcagctg ctgcgttgga
    44401 ttatgacatc agtactcgta aatatatcaa aaagagaatg ccttcgattg gtgtgatctc
    44461 tacgatcggc aaagcgagcg aagaacagga tatctattac aaacatttgc gagtagggat
    44521 ggaagaagaa gctcgacgtc tgcatttagg gatgaatcga atctataatc tatctgataa
    44581 tcctaaacaa tgggaagatc tagatcagtt aggtgcggtg atcgttgttg ggactgttac
    44641 gaaagaatcg attaccgcct tgttaaaaca aaacatgcaa atagtcgtcg tggataaccc
    44701 tgacatccag caagaggtcg atatggtgta tgcagatctt gaacggatga ctaaaaccgt
    44761 actggaattg ttcttagaaa atggtcataa aagaatagcc tatatcgggg gatataaagt
    44821 cgatatggat gaattcggtg ggaagcagat ttctataaat gaaaagagga tgcgtgccta
    44881 tcagcatttt atgatcgatc atcacttaga aaaatatatg tcttatcatc taggtggctg
    44941 gacagaaaaa caaggtgagg tacttgcgac taatctatta gaaaatgaat cccctcttcc
    45001 tactgccatt cttgtcggga gcgatccaat ggcaattggt gtctatcggg cattgaaaca
    45061 aaaaaatatc caaattggag aagagattgt tgtagcaagc tttgataata tcgagacaat
    45121 cgcaaacctc tcccctggct tgaccagtgt tcaaatcaat gcgaaatcaa ttgggcaagc
    45181 agctgttcga atggcattgg agcgaattga cggtatacgg gacgaaccaa tcattttgac
    45241 ttatcctgca cgattgattg ttagggaaag ttttatccca agaacaaaaa ggtattgaaa
    45301 gagaggttgt gaaaagccgc ttcgtttctt catactcaaa acgaaacggc tttttcacaa
    45361 cctcttactt atacttattc acaaaatcca atgtctgctg gtaacaattt tgcgattctt
    45421 ttagcgtgta atcaaattgg tattcatggg taatctcttt ttttgtatct gtataaaaca
    45481 aggattgcac gggtaccttt aattctgtca accgattttc taatgcttga ccttgctcct
    45541 ggaatgagta tgcatttccg tcagttatat acgttggcgg aaattcctta gaaacatggt
    45601 cagccacact agcttctttc aattgctcac tcgttttcca atcttttgtt ccaatcaacg
    45661 accacgcaac ggttttgacg aaaaacttca taaatcgatc atccgaatgc tgctgtgctg
    45721 ttagcttcaa atcaacaggt ccgcaataag aaatggttcc tttcagacta tcttttggaa
    45781 gtagctgttt gattttcatt tgtttagcat acgcatcatt cgtttgtata gctgcatatt
    45841 gcaatgcgat ttgtgaacct gcactatctc caccaaaaaa taattgttcc atgtctagca
    45901 atttgtcgtc tttttcttca gctaataaat ctttgatcag ttcattcact tgaagaactt
    45961 gattagggta ttctgaatca ggcgctaatt cataattcat cgcaaccact gcgatgttgg
    46021 cgtctgaaac taattttgtc gcaaattctt tgacacccga tttatctccg ccgacgaatc
    46081 ctccaccatg tacccaaaat agaacaggaa caggcccctt gctgtttttt ggatagtaga
    46141 tgtcataagt attctcttta tacttggact catacgtcaa atctgttttt ttcgccacgt
    46201 ttttttctgc ttgatcgaac gtttttttat cagtgatttg aacagtattt gaaaacatat
    46261 ggttgatcaa gaatgctcca ggtctggggg aaagctggaa ggcaagaaag attgcaaacg
    46321 cgatcccaag aatgattccg aagaaccatc cgataaattt aagaattttc tttttcataa
    46381 ggctccctca gtagttagta ttaagcattc tgattatacc atagtgttat ttcctatcaa
    46441 gctctcctag atgatttaat gattttcccc aaacttcttc tgcaatgctt ttgacaaaag
    46501 aaattttttc cattgttctt caaacaaaac attttcttct tctgttgaag caaatccaca
    46561 ttgaggactt aagcacaaac gatccaaagg cagatatcgt gtcgcttcgt agatccggtc
    46621 aatcacttgc tggtgctctt ccatttcgcc atttttcgtt acagtcaaaa tctaagacaa
    46681 ctaatttatc ttcagagact ttctctaaaa gaacaaaact cccagcacgt tcactatcgt
    46741 attctaaata ataggcacgg acattttctt gagtaaaata atgggtctgc tacgtcgtca
    46801 tcccccctga tgccgccaaa atcgaataat aatttccgcg tcaaacatgt atatttagtg
    46861 ttgaatcatt tgagaatatg aaaataaaaa tctttttgat caatcagtta atcaaattga
    46921 aataacgaaa aatctgagac agaatgacgt tcattctgac ccagatttga ttttgcacaa
    46981 ctcaacagtc cttattggca aatcgccaaa ataatgtaga ctaactaaaa agactaagaa
    47041 acaataacaa aagttaacac ttcttagtct ttctttagca cgtctattta ttaaagattt
    47101 tagggccttt tctcatgttt ttacgatcat tcttaggtgt ttccatttgt ttggatcggc
    47161 ctccaccttg tttctttttg atcaattctt tcattttttc tttttggtcc atttttattc
    47221 tcctttaacg aatgaagcga ctaattcgat atgatgggtt tgcgggaaaa gatctacagg
    47281 ctgaactttc ttcacggcat aacctgcttc tgcaaataac ttagcatctc ttgcaaaagt
    47341 tgccggatta catgagatat agacgattcg ttctggtgac atttcgattg ctgattcaat
    47401 aaaatgatga tctaatcctt ttcgtggagg gtcaactacg atcacagatg gtttgatccc
    47461 tttttcttgc cattttttca taactttttc tgcttttccg acttcataat gaacattttc
    47521 gattttatta tttaaagcat tgaactgtgc atcttcaatt gcttctggaa taacttccat
    47581 cccatacacc ttatccactc tgtctgctaa agacaagcca atcgtaccta ttcctgaata
    47641 agcgtcgatc acaatgtctt ttttatctaa ctcggcaaac tcgatggctg tttggtacag
    47701 atactctgct tgttgagtat ttacttgata aaatgacttt gctgagattc gataggtttt
    47761 tcctaatagc tgatcttcga tatagtctct gccatataaa actttttctt tgtctcctaa
    47821 aatcacattt gttttttctt cattgatatt ttggatgacc gaaaccactt ctggcaattc
    47881 ttcatgaata gcttcagcaa tttccttgga tttaaagaat ttttctttac gagtaactaa
    47941 aacgaccatc atttcatggg aataatgacc acgacggata atgatgtgac gaagctgtcc
    48001 ttcatttttt tcttcattat atcctcgtac ttggaaccgt tgcaagatat ctctgactac
    48061 tttgattgct tgatcgatga caggatcttg gatataaaaa tcttcgatag ggactagatc
    48121 atggctattc tttttgtaaa aaccggtggc taactgtcca tcgatacggc gaacagggat
    48181 ctgtgcttta ttacggtagc cgaatggttc tgccattccg accgttggaa gtacaggaac
    48241 ttctggcatc ttagcaatct tcgccattac atgctctact tgtttttgtt tgaagacaag
    48301 ctgttgttca tacttcaaat gggctaaagg tgcgatacct atgcgaagta atgcattgtt
    48361 ttctacaggc tgacgatcag gtgattgtgt aataaatgtt tcgacttttc catatccaaa
    48421 cttattgcct gcttttacta ctagtatttc tacttcttct ccagggagtg cattttcgat
    48481 aaataatgga taaccatcga tttttgctac ccccattcct tcataactca agtcgacgat
    48541 ttcgactgta tatcgttcgt ttttctttac tatttgctgt tctgtcatat tttgctcctt
    48601 taagcctatt tttcaatcct actgaactct actataaatc agctaattac acaactaaaa
    48661 ccggcactga ccttactacg ttcagcaccg gcttttgatt aattcttttg attaggtgtt
    48721 tcgtctgcta tttttccgtc accgtcgata tcttcatccg ttaaacgttc gacttctttt
    48781 acgaattctt tgctgacaac ctctaattct tcttcatctg tacctgtgat tgcttcatca
    48841 ggaatctgat cggtattggc aaagaactca atgtgttgat gaagattttg gaaatgcatc
    48901 ggtgcttccc cgccgtattc accatctaag ttgatcatta ctggtcgatt gatatcatct
    48961 tctgtttcaa cgaacaaact gcttgttttg gtatagatta ttcttggatc atcgacatgt
    49021 cgaccgccat tcagcatgag tgctgcaata tgcaagatct caaagatatt tgctgtcttc
    49081 acaatgatta aagaaaactt cccatcatcc aatttggcat ctggcgcgat ctgttcgaat
    49141 cctccaacag agttcgtcag ccctaggaaa aacatcgaag catttccgat atattcccct
    49201 tcatcatact tcaagcgcat tttgattggc tttactcgag ggagcatctc tgctcctttc
    49261 gcaagataag caagataacc aaaaatactc tttagttctg atggaacttc ataagtcaat
    49321 tctgtcaaat agccacctgc agcgatattg atgaagtaac tgttatcaga aaggccgatg
    49381 tccattttta cggtttggtg tttttttatc acttctgctg ctgctttaat attatctcta
    49441 ggaattttta atgctcgagc atagtcgttc gttgtaccag ctgggatgat ggccatttta
    49501 ggacggtgtt caagcggagc gattccatta accacttcgt tgattgtgcc atcgccacca
    49561 gctgccacta ctagatcaaa acctagttct gcgactctac atgcttcatt cttggctgaa
    49621 tctggttctg gtgtagttgc ataggcgctg gcttcaaatc ctgcttcttc taatgcctgt
    49681 aggatatccg ccaaattttt tttcaataat tcttttcccg aggtggggtt ataaattacg
    49741 cgtgcttttt tcattttcag cctctatcgt ttcgctaatt catctttaag tagcttattc
    49801 acgacaccag gatttgcttg tcctttagtt gctttcatga tttgaccgac taggaagcca
    49861 accgcacgat cttttccatt tttgaaatca tcgacagatt gttggttgtt atccaaaatt
    49921 tcattaatga ttggcagtag tttgttcgga tcagataatt gcacccaacc atttttttca
    49981 actacttctt gggcttctcc gccatttgtg atcaattcac ggaagacttt tttggcgatt
    50041 tttgagctga tcgttccatc ggcgatcaaa cgaatcattc ctgctaagtt ctcaggtgtc
    50101 aattttgtct cagctaattc cagtttctca ctgttcaagt aagcagaaac ttcacccatc
    50161 aaccagttag atgcttgctt ggcatctgca ccattttcca aaacagcttc aaaaaaatca
    50221 gacatttctt ttgtaagtgt caataccatt gcgtcatact ctggaagtcc aaactcacgg
    50281 atatagcgtt cgcgacgaga agctggcatc tcaggcaaac tagcttttac tttttgaatc
    50341 cattcgtcat cgatcacgaa tttcgggata tcaggctctg ggaagtaacg ataatcacta
    50401 gacccttctt tgacacgcat caaaactgtt ttattcgttg tttcgtcaaa acggcgtgtt
    50461 tcttgttgga tttctcctcc tgaaagaaga acttttgctt gacgtttttc ttcaaaagct
    50521 aaaccttttt tcacgaaaga taaagagttc aagtttttca attctgcttt cgtaccgaac
    50581 tcttcttgtc cataagggcg aagagaaata ttggcatcac agcgcataga gccttcttcc
    50641 attttcacat cgctgacttc tgtaaattgg ataattgatc gcaatgcttc tagataagcg
    50701 tatgcttctt ctggtgaacg catatcagct tcagaaacga tttcgatcaa aggcgtgcct
    50761 tgtcggttca aatcaacgta agaataaccg ccaataccat gcatgttttt tccagcatct
    50821 tcttctaaat ggacacgttc gatccggatt tttttctttt taccttctac ttcgatttcg
    50881 atccatccgt catgaccgat tggttgatca aattgggaaa tttgatatgc ttttggatta
    50941 tctgggtaaa agtaattttt ccggtcaaaa tgagtatctt tagagatttc acagtttaat
    51001 gccaaagctg ctttcatccc gaattccagc gcagctttat tcattactgg cagcacgcct
    51061 ggataacccc agtcgatcac gtttgtattg ctgtttggtt ctgcaccaaa atgagctggc
    51121 gctggagaga aaatttttga gttggttttt aactcgacgt ggacttctag tccaatgact
    51181 gtttcaaaat tcattcgtct ttccctccta aaatagctgg ttgttgttta tggaattctg
    51241 ttgcttgttc aaacgcatat gccgcttgat acatcgtttt ttcatcaaaa taattaccga
    51301 tgatctgcaa tccaactggt aatccttctg ataaacctgc cggtacagac atacctggaa
    51361 gtccagccaa gttgacaggg atcgttaaga tgtcactcat gtacattgtg attggatcgt
    51421 taatgttctc accgattccg aatgcaactg ttggtgaaga tggcccgatg atcaagtcat
    51481 aatctgcaaa gactttttca aaatctcgtt tgatcaatgt acggacttgt cctgcttttt
    51541 tgaagtaagc gtcataataa cccgcgctta aggagaatgt acctaacatg atacggcgtt
    51601 ttacttcttc accgaatcct tctgaacgtg agttgacata tacatcatct aatgatttca
    51661 cattttcaga gcgataaccg tagcggatac catcgaatcg ttgcaaattg gaacttgctt
    51721 ctgatgaggc aatgatataa taaacagcca caccgtattt tgaatgaggc aagctgactt
    51781 cttccacaac agcacctaat gaacggaatg tttctgctgc tttcaataca gcttctttta
    51841 cgccaggggc cacaccttcg cctaagtatt ctttaggtaa agcaatcttc attcctttga
    51901 tgtcgcctgt cagtccctct gcaaaatcag gaactgaaac gcctgaagaa gtaccgtctt
    51961 tttcgtcata accgctgata gctgtcaatg ctaatgcatt atctttcacc gtacgggtca
    52021 ttggtccgat ttggtcaaga gatgaagcaa aggcaatcaa accaaaacgt gagacacggc
    52081 cataagtagg tttcatccct acgatgccgt taaaagctgc tggttggcgg atacttcccc
    52141 cagtatcact acctaaagaa actggaattt gtccagctgc aactgctgct gcagaaccac
    52201 cagaagagcc accaggtact tttgttgggt cccaagcatt ttttgttttc ttgaaataag
    52261 aagtttctgt acttccaccc atagcaaatt catccatgtt tagcttacca acagggatca
    52321 tatctgcttg atagatttta tccatgaccg ttgcatcata aattggttca aaattgtaaa
    52381 ggatcttaga tgcagcggtt gtcaagatat cttttgttac gatattatcc ttgataccaa
    52441 ttggaatacc agcaaggacg ttgctttcat caatcccttt ttcatctaaa gcttttgcct
    52501 gttccaatgc tttttcttcg ttcaatgtaa tgaatgcctc gacctcaggc tctgtttctt
    52561 tgattcgttc aaatgttgtt tttgtcaaat cttgtacagt gatttctttc gatacaagca
    52621 tatgatgcag ttcttctaga gaacgattgt gtaattctgt cattatgcgc cagcctcccc
    52681 attatcgatg attgcgggta ctttgataaa gccgtcttct gtttccggca catttttcaa
    52741 taattcatcg cggttccatc cagcttgcgc ccgatcttgt cgcatcacgt ttactgtttc
    52801 aaccacattc gacgtaaatg gaacaccttc cgtatctact tcttccagca tttcaaccat
    52861 atcgatgatt ttacctaatt ggtcggtaaa acttgcaagt tcttcatctg cgaacgcaag
    52921 ttttgctaat ttggcaacgt gtttcacttg ttcttcacta atcgccatac taccccttct
    52981 ttctaaacga gttaaaaact ttgctctcta ttttaacaca agagtgccgt gtctgaaatt
    53041 gtttattgaa atttgtctga aaaaactcat ttttcattct tctactgagc gacttttagc
    53101 caatgcatct gccatttgat cttctgtcca aacttcgatt cctaattctt gtgctttggt
    53161 cagcttgctg ccagcatctt ctcctgctac cacgatatcc gtttttttcg atacgctacc
    53221 agtcactttc ccgccaagat tttcgatcgt ctcttttgct tcttcacgag taaatcgtgt
    53281 tagtttacca gtaaggacta ctgttttttc tttaaaagga gactcgactt cttgcaattg
    53341 ggcatttcga agccctttgt attcgaaatt gacgcctgct tgttttaatt cattcatcaa
    53401 ttcatggact tcttcgttac tgaaataagt gactacactg tcggcaatga tatcaccgat
    53461 cgtatcaagc gcaataatcg actcatagtc acttttactc aatgtttcta gatcaccgaa
    53521 atgttctgct aaaatttttg ctgctttcgc tccgacatgt cggattccta acccgaaaat
    53581 caaccgctcg accgaattat ctttgctgtt gtcgattgct gtcaaaatat tatttgctga
    53641 tttttctttt attttgtcca acgtcaataa ctcttcttct gtcaatttgt aaagatcagc
    53701 aacatctgct actagttttt tatcatacat ctgttctagt acgcgcggcc ccaacccatc
    53761 gatattcatc gcatttcttg aaacaaagtg gttcaaacct tctttcattt gagcagggca
    53821 tttaggattg atgcaccgta aggctacttc ttcatccaaa tggacaagtt cacttccaca
    53881 aactggacaa tgtgtaggca attggtattc ttcgctgtct ttaggtcgtt tatctaacac
    53941 gacttgcgaa acttctggga tgatgtctcc cgctttataa atcagaactg tatctttcaa
    54001 gcggatatcc ttcaacttaa tataatcacc gttatgcagg ctggcacggc ttactgtcgt
    54061 tcctgctact cggactggtg tcatgatagc tgttggcgtt actacccctg ttcgaccgac
    54121 tgtccattcg atgttttcga ttagcgtttc aacttcttct ggcggaaact tgtaagctgc
    54181 tgcccatctc ggtgctttga tagtaaaacc aagttgatct tgtagcgaga attcattcac
    54241 tttgatgacg attccatcga tttcataggg caaatccaca cgtttgtcat gatattcttc
    54301 aatgtatgac cacacttcat caatcgaatg acatagacgt ttttcccgat tcgtgtggaa
    54361 tccaattttt tctagctctt ctagcgcatc atactgggtt tttgcttgca taggaccgaa
    54421 atcagctact gtgtataaaa aagtgtccag atttcttttc gctgtgattt tagaatctaa
    54481 ctgacgcaaa gaaccagctg cggcgttccg aggattcgca aagatatctt ttccctctgc
    54541 ttctctgtct tggttcaatt ggacaaatga ccgttttggc atgaaacatt cgcctcttac
    54601 ttcgatgttc attggttctt ttaatttgat tgggaccgac cggacagttt ttaggttttc
    54661 agtgatgttt tctcctacag ttccatcccc gcgagtcgca cctctcacga aatttccatc
    54721 ttcataacgc aaagaaacgg aaagtccatc tatttttagt tcacagcaat agtcaactac
    54781 acgtcccact gctttttgta cacgttgatc aaaagctatc agctcttctt tgctgaaaac
    54841 gtcgttcaaa ctgtaaagcg gaatatcatg agtgactttt tcaaaacctt ctaatacttt
    54901 tccgcctact cgttgagtgg gagaatcaga cgtgatcaaa tcaggatatt gttcctcgat
    54961 tgctactaat tctgcgtatt ctttatcata aacatagtct tccactgttg gtttatcttc
    55021 tacgtagtat tcgcgagacc attggttcag tcttgtacga agttcttctg ctcgtttagc
    55081 agcttcgtca atcgtcattt catgttccat catttaccct ccactactaa gctttttcaa
    55141 ttggcgcaaa ggcagccaaa agacgtttta ctccttgact tgggaaagcc acatctagtt
    55201 ctagatcctt cgacgtcccg cttactcgga cgactgttcc tagaccccat tttttgtgtt
    55261 tgactttatc tccaggtttc cagctgttat tttcccctcc agtagcactt ttagagggaa
    55321 caaccgctgt ctctggctgt ttatagctat atgctgtttt tttagcgaat acaggcgttt
    55381 ttttaggcgt aggacgcatg ccttcgattt ccagtaactc ctgatcgatt tcttctacaa
    55441 aacgagaagg tcggttatat tgcgtccgtc catataatgt tctggagaaa gcatttgtta
    55501 aatacaacgc ttcttctgct cgcgtgatcc ccacatatgc gagacggcgt tcttcctcca
    55561 gttcgctttc ttccatcaat gctcgagata aaggaaaaac cccctcttct agaccaatca
    55621 ggaatacaac tggaaattct aatcctttgg ctgcatgcaa agtcatcaat gtcacttggg
    55681 aagcatcttc ttctaaattg tcaatgtcgg aaacaagtgc cagatcattt aagaagaccg
    55741 ttagtttttc ttctggtgca tcagcgtctt cttcattttg ctgttcgaat tgtttgtcga
    55801 attcttgtgt gaccgttaaa aattcctcca agttttcgat gcgtgcttgt gcttctaatg
    55861 tattttggat ttttaagtct tctagatatc cgctacgatc caaaacctct ttcgtcaatt
    55921 ctgtcactgt caagtatggg atcatctgag tgacttcttg gatcatctct ccaaatgcac
    55981 ctaattgctg tcctgctttt ccaccgatat ttgccagatc cacattttgc gctgcttcaa
    56041 gcagtggcca ttcatgaaga gaagcaaaac tgcgtagttt ttcgatagat cctggtccga
    56101 tgcctcgttt gggcgaatta acgatacgct caaaactgat tgaatcttga ggatttacca
    56161 gcacattcaa atacgctaaa atatctttga tttctttacg atcgtagaac ttgtgccctc
    56221 caaccatttt ataaggaata tttgctttca acagagtttc ttccattaca cgagattgtg
    56281 cgttcgtccg gtacaagatg gcaaagtcgc cgtaattccg atgattgctt cgaatctctt
    56341 cttgcatccg gtctacgata aaacgggttt cgtctcgttc attatctgca cggtagtaag
    56401 tgatcttgtt tccttctttg ttttctgtcc acaagttttt tggttttcga ttgctattgt
    56461 tttcaatgac ttggtttgct gcgctaagga tgtttttcgt ggagcgatag ttttgttcta
    56521 gcaaaattac tgcagcatct ggataatctt tttcaaaatc caagatgttc tgcatatctg
    56581 caccgcgcca gccgtaaata ctttgatctg catctcccac gacgcacagg ttcctaaatc
    56641 tacctgccaa taaattgact agcgtatact gtgcgtgatt tgtatcttgg tactcatcta
    56701 catgaatata atggaatttg ttttgataat acgtgagtga atcaggatgt tcttcaaata
    56761 atcgaatcgt attcatgatc aggtcgtcaa agtccataca ttgattatat tgaagctctt
    56821 tttggtaagc agcataacat tttgccgcaa tctcttcgta ataactgcct tgcatttttg
    56881 caaaatcctg aggggtctgc aggctgtttt ttgcttgaga gattgttcct aatatacttc
    56941 gaggatcata ttttttcgga tcaatgttca gttcttttag tatccgtttc atcaaggtca
    57001 gctgttcaga agaatcgatg attgtaaagt tccggttata cccgataaaa tcaacatctc
    57061 ttcttaaaat acgcacacac atcgagtgaa aagtcgatac ccagacatct tcgcctccag
    57121 atgcgagaat cgcattgaca cgttctttca tttcttttgc agccttattg gtaaaagtaa
    57181 tcgctaggat gttccaagga ttgacttctt tttcctcaat caaataggca atgcggtgtg
    57241 tcagcactct cgtctttcca cttcctgcac ctgccattag cagtaatggt ccgtcagtgt
    57301 gaagaacagc ttctttctga cgggggttca tcccgtttaa taattcagct ttggatgaca
    57361 ttaacaacat cctttctttt atcaatcttg ttaattatag cattttcttg gctttcatta
    57421 aactagctag ttcaaaatca tttgtttctt tttatggatt tttccttgtc aattcgttga
    57481 tttcgcgcta aattaaaaga aagcgaggcg tttgaatgga aaaaaaagga aaaatcaaga
    57541 aattagataa gactcagata tattttattc taaaaggaat atttgttgga ctgctgacag
    57601 gaatcgtcgt tagcctgttt cgattatcta tcgaaaagct aagcgattta gttcgaagta
    57661 tctatgaaat gagtcgggaa cagccgattt acttaattgg cctagctatt tgctgcatcc
    57721 ttgcagcatt tttcgtgggt tatctggtca aaaatgaacc ggatatcaag ggaagcggga
    57781 ttccacaagt agagggacag cttcgcgggg aattatcaat gaactggttc tctgttttat
    57841 ggaaaaagtt cattggcggt gtactttcag ttggtgcggg cctttttcta ggtagagaag
    57901 gaccttctat acagcttggc gcatccattg gacaaggagc tggacaattg tttcggagtc
    57961 catcttcaga agaaaagatt ttaatttcta gcggtgcaag tagcggactt gcagcagcat
    58021 tcaatgctcc tatcgcagga ttactctttg ttttagaaga aatccatcac tccttttctc
    58081 cacttgtttg gctgacatca tttgcttcag caatcactgc taacttcgtc tctctttact
    58141 ttttcggctt gagacctgtt ttatatctag gtgaccttcc ttctttgccg ttgaaatatt
    58201 atggttcctt agtgatctta ggagtacttc taggcatctt aggttttatt tatcagaatg
    58261 tcttgctggc tttgccaaaa ttttacaacc gattgccttt accagcttat tttcacgggt
    58321 tcgttccttt cttgctgatt ttgccaattg gttatttatg gcctagtact ctaggcggag
    58381 gcaatcaaat cgttttagct tttggacatg cttccttgcc aatcattatg attatcggtt
    58441 tgtttgttct tcgattcgtt ttttccatga tttcttacgg tgggggtctt ccagggggga
    58501 ttttccttcc gatattgaca ttgggggctt taatcggtag cttttatgga aacattatgg
    58561 ttgatttcgg gatggatcca atctacatca aggattttgt cgttattgcc atggctggtt
    58621 atttcacagc gattggaaaa gcaccgctga cagcaattat tttagtgaca gaaatggttg
    58681 gctctcttaa tcatctgatg cctttaggat tggctgctct tgttgcttat atcgtcaatg
    58741 accttctcgg cggtaatcca atttacgaat cattattgga acgtctactt ggaaatactc
    58801 agaccgcctc actaaaaggt gcaaggacaa cttttgattt tccagtaacg gcagagagtt
    58861 ctttagacgg attaatggta cgagacttca attggcctaa aaatatgctg ctaatctcta
    58921 tacgcagagg aaatcaggaa atcttgactc acggtgatac gatcatgaat gtaggagata
    58981 ttttggttat cttgactgat gaagcaaact tgccaagaat caagcaggaa atcgaggcta
    59041 aatcttctat cacaaatttg aagcaatcat tttaacgaac atggaaaaac tattttacaa
    59101 caactattca tctacactac ttgattctat atgattctat aaaaaaagaa gaggctggga
    59161 aaacccagcc tcttcttttt tatttctatg cttctgctac tgcaggagct tttctcaata
    59221 aaccataaat cacaccagat acgactgctc cgatagcaat aaacaccaaa tacatgaccg
    59281 ggtgactcag taagaataca acgaaaatcc cgccgtgagg agccagtaat ttgattccga
    59341 aagctccgac taatccgcca gttaaagcag aaccaataat aaaacttgga atggcacgaa
    59401 tcgggtcagc tgctgcaaat ggaatcgatc cttccgtgac aaacgatagt cccatcacga
    59461 tgttcgtcaa accagcatct tgatctgttt tgctgaattt attcttaaac aaacgtgtag
    59521 caacgaaaat tgccaatgga ggtaccattc ctccagccat aacagaagcc atcacgacac
    59581 ttccgccagt tgcaacagtc gtagctaaag ttgctgtacc aaacacataa gcggctttgt
    59641 tgattggacc gcctaaatct gccgccatca ttccagctaa taatgcacca agtaaagcag
    59701 cattcgaacc actcatactt gtcaagaaac cgttcaatgc atcattgatt gctttcatag
    59761 ggacattgac cagcaacatc aagaaaccag taatcagtaa gccaaacact ggataaaaga
    59821 ggatcgtttt aattccatcc aatgattttg gtaagttctt gaataattta cgtaaaaata
    59881 cgatgacata accagctaag aatccaccaa tcagtgcacc taagaatcct gcaccgcctg
    59941 tattagctaa tgcaccagca gcaaatccaa cgatcaaacc agggcggtca ccgatacttg
    60001 aagcaataaa tcctgctaat accggcagca tgaaaccaaa tgcagcttgt ccgatttggt
    60061 tgaaccagct agcagcttga ttgtaattcc ctagatttga taattgatca tgtggcacgc
    60121 caataaattg gtcgatcatg aaagaaagcg caatagcgat tcctccacca ataacgaaag
    60181 gtaacatgtg tgatacgccg ttcatcaaat ctttgtaaat tctttgtccg atagtaccat
    60241 ccgcatttcc ttcttctgaa ctgctttctt ttccatcacc atggaatact ggtgcagtac
    60301 cactaagggc tagattgatc aactcttctg gtttacggat accatcactg acaggacgat
    60361 tgatcagttc ttttccgtca aagcgattca tctctacttt tttgtctgct gcaacgatta
    60421 caccttctgc acgggcgatg tcttcagcag tcaatcggtt tttgattcct tctgaaccgt
    60481 tcgtttcgac tttgatctcc acacccattt ctttcgcttt tttcttcaaa gcatcttctg
    60541 ccatgtaagt gtgagcgatc cctgttggac aagctgtaac ggcaacaacg aattttcttt
    60601 ctggatgagc agaagcttct gcagcttgtg tttcttgttc tgctttttct tctgcttctt
    60661 tttgctcttc agctgtttgg aacaagtctt ggacttcttc tggtgtattt gcttctttta
    60721 attttcctac gaaatctgca tcaatcagca atcgagatag tgctgctaat gcttgcaaat
    60781 gtgtgtcgtt tgcgccttct ggagctgcga tcatgaaaaa cagataagtt ggctgtccgt
    60841 ctaaggcttc ataatctact cctttttggc tttttgcgaa taaaactgtt gcttctttaa
    60901 ctgcactgtt tttcgcgtga ggcatggcga ttccatcacc aagacctgta gaagtttgtg
    60961 cttcgcgggc tagaattcct tctttatatg tttcgatatc ggaaatccgg ccggcatcaa
    61021 acattttttg gaccatttca tcgattgcgc cttttttatc agtcgcttgc aaatccatga
    61081 tcatcgcttc tttgaccatt aaatccttga tattcatgat tatcctcctt gaaactaatc
    61141 aatttttgtg atttcaactt ctggcagcaa ctcatcaatc aattcgcgag ctgccaagtc
    61201 atcagaaaaa gctgttgcgc ttccgcaagc aactccccat ttaaaggctt cgattggatc
    61261 tttcgtacgg ctgtaagccc caataaagcc agcaatcatt gaatcccctg cacctactga
    61321 atttttcaat gggcgaacta atacattcgt tttataagtt ccttgttccg tgaagagcaa
    61381 agcaccgtct ccagccattg agatcagcac attctgtgcc ccttcagcta gtaatttttt
    61441 cccatacgga atgatttctt ctacagattg gaaggttgtg ttgtaaagct cagctaattc
    61501 gtgattgttt ggtttgatca acaacggttt cttaacaagt gcatttttta ggtcatcacc
    61561 tgttgtatca atcacgaaat ctgcaccttt ttctcgaatg atatcaatca gttcttgata
    61621 aaatcctttt cgcaggctag ctggcgcgct tcctgacaat acgacgatat ctccttgtcc
    61681 tactgttcct aatgcttgct tcaactcatc gatttcttca tctgaaatag atgggcctaa
    61741 gccattgatc tcagtttcag tgtctgattt tagtttgata ttgattcttg tatcttcttt
    61801 tacggttgtg aacaaagact ggatttgttc tttttcaagc cacttcgaaa taaattcgcc
    61861 agtgaaccct cctaaaaacc ctagtgctgt agattgagaa tcgatccgtt tcaaaatacg
    61921 ggaaacattg atcccttttc ctccaggtaa cttgaagtct tccttcattc ggttcaaatc
    61981 gcctagtttt aacccatcta cgcgtacgat ataatcaatt gatggattaa gtgttactgt
    62041 gtaaatcatt gtattgcctc cttaattgtt gtcttttcac ggaattcatt aacgaattct
    62101 aatggacaac gctcggttat gatcgtggct gcttccaaag gtgcaacaga ggtgaaggac
    62161 aactggttga atttggtatg atcaatcaat acaaatgtct cttcggaatt tttcatggct
    62221 aaacgtttaa cagctgcctc ttctggatcc ggagtcgtga atccttgttc aagatgggca
    62281 ccgttcattc ccataaatgc tttattaaaa tgaaaatttt ttagttgttc tatcgcatta
    62341 gatcctaaaa cagcattagt tgccagcttt atcgttccac ctaaaataat ggtagcaagc
    62401 tccaaatcaa ctaaatttgc cgcatgttga acggaatttg tcactactgt gatctgtttt
    62461 ccttgcagaa aaggaatcat ttctaacgta gtagagccag cgtctaaata aatgacatct
    62521 tcgtcattaa tggtttcagc tgccgatttg gcgatttgct gtttttcgtg aacgtttttg
    62581 agtgattttt cactcatatt ctgctcaaaa ttcaataatt gctctttctt agctccgcca
    62641 tgaactcttt ctagaaaccc actattctcc agctcttgta aatcacgtct tatcgttgat
    62701 tcagaagcat tcaaaaggtt tacaagatct tgtgatttca cgattttctg ctgttcaagc
    62761 aagtctagga ttttttgtct gcgttcctct gtaagcattt tcacgatcac ctcaatacaa
    62821 ataatagcat attttttttc tactagcaat ataaatcagt caaaaacgtt cataaaaatt
    62881 ataaatcagt caaaaaaaaa ataaaaaacc agtcagaaag actggttttt tatttttttg
    62941 tctatcatca agccgtttta tcgtttttta ttttcgcttc ttgttttttt catataaaca
    63001 gaagcaggtt ttctttcctt atgaagaaga taataataaa gtcccatccc gccaaacatg
    63061 accaaaaaac cgatgactga tcgaatagga aaattaggaa caaaaaagag acagacggat
    63121 aataatgatg ccggaaccat tattcgataa tagagttgtt tatccatttt cttcacctca
    63181 tattttttgt aaagtaatca attgttctaa ttctcggtaa tctaaaaatt gcacacgcag
    63241 atgctgctca cctttcttga catcgaattg gatacttcgc cgctttttct ttattcgttt
    63301 gaaggtaaca tcagagagtc tatgtttgat ttcctttaac ggtaattctg acagggattc
    63361 tgaaggaaca tagattctta aggataaaag atactcttga tatttttcat ctgttacctc
    63421 tacttctaga tattccctaa tataataaca ataagcttcg attccattat ccaaagcaac
    63481 agtcggatcg ataactttat agcacatcat gcgtgatcga ttctcgtcaa cccaaggaaa
    63541 atacgcctga gcatttagtg ttccgcgaac acgcctttct ttataatctc cttgttggat
    63601 atcataactt tttgttacaa aaaatgaagc attcttttta ggaacgggtt gcatttgtgc
    63661 attctgatac tggacagatt ggatcgtatg gtcagtgtaa atgtattcaa cgaatggcgg
    63721 tttcaataaa ccgacaccat tgtcccaagc aagatgatca aacccaagct ggtccctaaa
    63781 ataggattga aacatttgat tgtttggctt ttgccagtta aaggggtctc gataaagctg
    63841 gatacatatg acttcgtttt ctaattcctt cactttttct acttgttgga tgtcgagaaa
    63901 aatcggttta gggatgattt gatgataata gcgactataa tacatttccc aacacgaagt
    63961 gaaacataaa ccttggtaaa aaagatcgta tcctggaaat tggttgcaat cgactactac
    64021 ttccccgttt tgttgtttca ttttaggtaa atgttcgatt tcatcctttt tctcaaacaa
    64081 tagccgttga gagatctgtt ttgtattgtg atagataaac tcgctataag ctcgcatata
    64141 ggcgaacacc ccatgagcaa tcattttttt ctgaatgaag tctcgaatca aaaattgctt
    64201 ttctttgaaa acagagatag acaatacatt ttttatcaat aattgattat cttccaagcg
    64261 aaaactacat tcgttctcgt catttcctac aactaaccaa ctttcgtctc ttaccgatgc
    64321 tagcattatt ttttcttgaa gctttcgaag atcatacgga tccgcttgtt gtgtgaaaga
    64381 gcggataaat gttggctgga tcaacagacg tgtcaaaata gcccaatcgc ttatttcctg
    64441 ttttttttct tcttcattta agtctgtgag ctggatagtc atcaataatt tatcttcgcc
    64501 aatcattttc tcacctcttt caaatctttt tataatttaa ttataaccaa tgataaagaa
    64561 aaactaaaac tttatgtctt agaaaccaat ttggacgatt acatgataga ctaagtaaaa
    64621 agaaacaaag aggtgtttac aatggggttg aaatttgaac gtacagataa tttagataaa
    64681 cttttcgaat catttgctgt cgatccggaa aaaacagttg caccaaaaga agagatcgat
    64741 cgatttctaa aaccacagcc acaaaatgaa aagaaaaaag acaagaaaca aaagtaagtt
    64801 aagctcaaaa aa
//
LOCUS       DAFEPQ010000009        61055 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.009, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000009
VERSION     DAFEPQ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.009"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            212..604
                     /locus_tag="LP543_000632"
     CDS             212..604
                     /locus_tag="LP543_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL2320821.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="LP543_000633"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="LP543_000633"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBL2320822.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="LP543_000634"
     CDS             3165..4775
                     /locus_tag="LP543_000634"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBL2320823.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="LP543_000635"
     CDS             5067..5936
                     /locus_tag="LP543_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBL2320824.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="LP543_000636"
     CDS             6101..7372
                     /locus_tag="LP543_000636"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBL2320825.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="LP543_000637"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="LP543_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBL2320826.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="LP543_000638"
     CDS             8763..9026
                     /locus_tag="LP543_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBL2320827.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="LP543_000639"
     CDS             9471..9920
                     /locus_tag="LP543_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2320828.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="LP543_000640"
     CDS             9977..11395
                     /locus_tag="LP543_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2320829.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="LP543_000641"
     CDS             11395..12255
                     /locus_tag="LP543_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBL2320830.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="LP543_000642"
     CDS             12429..13202
                     /locus_tag="LP543_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL2320831.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="LP543_000643"
     CDS             13349..13714
                     /locus_tag="LP543_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBL2320832.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="LP543_000644"
     CDS             complement(13862..15070)
                     /locus_tag="LP543_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2320833.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="LP543_000645"
     CDS             complement(15089..15367)
                     /locus_tag="LP543_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320834.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="LP543_000646"
     CDS             15521..16216
                     /locus_tag="LP543_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL2320835.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="LP543_000647"
     CDS             complement(16658..17455)
                     /locus_tag="LP543_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBL2320836.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="LP543_000648"
     CDS             complement(17452..18636)
                     /locus_tag="LP543_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBL2320837.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="LP543_000649"
     CDS             complement(18661..19908)
                     /locus_tag="LP543_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBL2320838.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="LP543_000650"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="LP543_000650"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBL2320839.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="LP543_000651"
     CDS             21888..22202
                     /locus_tag="LP543_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBL2320840.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="LP543_000652"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="LP543_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBL2320841.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="LP543_000653"
     CDS             22887..23531
                     /locus_tag="LP543_000653"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBL2320842.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="LP543_000654"
     CDS             23546..23875
                     /locus_tag="LP543_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBL2320843.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="LP543_000655"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="LP543_000655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBL2320844.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="LP543_000656"
     CDS             24863..25687
                     /locus_tag="LP543_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBL2320845.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="LP543_000657"
     CDS             25680..26027
                     /locus_tag="LP543_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBL2320846.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="LP543_000658"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="LP543_000658"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBL2320847.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="LP543_000659"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="LP543_000659"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBL2320848.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="LP543_000660"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="LP543_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBL2320849.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="LP543_000661"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="LP543_000661"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBL2320850.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="LP543_000662"
     CDS             31657..32451
                     /locus_tag="LP543_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2320851.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="LP543_000663"
     CDS             32441..34483
                     /locus_tag="LP543_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2320852.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="LP543_000664"
     CDS             34764..34913
                     /locus_tag="LP543_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBL2320853.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="LP543_000665"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="LP543_000665"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBL2320854.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="LP543_000666"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="LP543_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBL2320855.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="LP543_000667"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="LP543_000667"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBL2320856.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="LP543_000668"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="LP543_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBL2320857.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="LP543_000669"
     CDS             39330..40412
                     /locus_tag="LP543_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBL2320858.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="LP543_000670"
     CDS             40412..41215
                     /locus_tag="LP543_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBL2320859.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="LP543_000671"
     CDS             41285..42025
                     /locus_tag="LP543_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL2320860.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="LP543_000672"
     CDS             42036..43040
                     /locus_tag="LP543_000672"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBL2320861.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="LP543_000673"
     CDS             complement(43085..44929)
                     /locus_tag="LP543_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320862.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45141..45659
                     /locus_tag="LP543_000674"
     CDS             45141..45659
                     /locus_tag="LP543_000674"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL2320863.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45787..46470
                     /locus_tag="LP543_000675"
     CDS             45787..46470
                     /locus_tag="LP543_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBL2320864.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46779..46852
                     /locus_tag="LP543_000676"
     tRNA            46779..46852
                     /locus_tag="LP543_000676"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46813..46815,aa:Arg,seq:tct)
     gene            47011..47370
                     /locus_tag="LP543_000677"
     CDS             47011..47370
                     /locus_tag="LP543_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBL2320865.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47493..49124
                     /locus_tag="LP543_000678"
     CDS             47493..49124
                     /locus_tag="LP543_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBL2320866.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49186..49827)
                     /locus_tag="LP543_000679"
     CDS             complement(49186..49827)
                     /locus_tag="LP543_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBL2320867.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    50013..50089
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            50109..50417
                     /gene="rplU"
                     /locus_tag="LP543_000680"
     CDS             50109..50417
                     /gene="rplU"
                     /locus_tag="LP543_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBL2320868.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50444..50788
                     /locus_tag="LP543_000681"
     CDS             50444..50788
                     /locus_tag="LP543_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBL2320869.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50801..51094
                     /gene="rpmA"
                     /locus_tag="LP543_000682"
     CDS             50801..51094
                     /gene="rpmA"
                     /locus_tag="LP543_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBL2320870.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            51528..54335
                     /locus_tag="LP543_000683"
     CDS             51528..54335
                     /locus_tag="LP543_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL2320871.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            54526..55017
                     /locus_tag="LP543_000684"
     CDS             54526..55017
                     /locus_tag="LP543_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2320872.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            55120..56109
                     /gene="manX"
                     /locus_tag="LP543_000685"
     CDS             55120..56109
                     /gene="manX"
                     /locus_tag="LP543_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBL2320873.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            56142..56945
                     /locus_tag="LP543_000686"
     CDS             56142..56945
                     /locus_tag="LP543_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBL2320874.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            56966..57880
                     /locus_tag="LP543_000687"
     CDS             56966..57880
                     /locus_tag="LP543_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBL2320875.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            57963..58337
                     /locus_tag="LP543_000688"
     CDS             57963..58337
                     /locus_tag="LP543_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBL2320876.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            58476..58721
                     /locus_tag="LP543_000689"
     CDS             58476..58721
                     /locus_tag="LP543_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL2320877.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            58893..59957
                     /locus_tag="LP543_000690"
     CDS             58893..59957
                     /locus_tag="LP543_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBL2320878.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            60203..60628
                     /locus_tag="LP543_000691"
     CDS             60203..60628
                     /locus_tag="LP543_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL2320879.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            60621..>61055
                     /gene="nusB"
                     /locus_tag="LP543_000692"
     CDS             60621..>61055
                     /gene="nusB"
                     /locus_tag="LP543_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBL2320880.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKEL"
BASE COUNT        19636 a        10485 c        12851 g        18083 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    45001 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    45061 tttttacagc tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat
    45121 aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa
    45181 cgttgattat atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt
    45241 tgactcctta tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga
    45301 tatatgtacg accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg
    45361 aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag
    45421 atttttatac aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa
    45481 atattatcgg tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg
    45541 catttgcatg gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag
    45601 catggaatac ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag
    45661 aatgttaaaa acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga
    45721 gaaaacactc atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg
    45781 agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt
    45841 atgcgtaccc agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat
    45901 gtagcagaag taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga
    45961 cagcttcagc ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc
    46021 atggccacga caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg
    46081 atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt
    46141 gaaggtcaag cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg
    46201 gatagtgcaa aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa
    46261 ggtggcgttt tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa
    46321 gagattcccc gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg
    46381 atggatcatc ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg
    46441 acaaaggaat cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg
    46501 attgaatcga gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta
    46561 aaggatacag gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc
    46621 aattgataac cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag
    46681 ttgggccaaa ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa
    46741 tttaattatc tataattatt gatgttccgc tgattcttgt catagtagct cagctggata
    46801 gagcatccgc cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa
    46861 ccgttagaaa ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt
    46921 ttcaaaggca aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa
    46981 ataaaactca tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact
    47041 ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac
    47101 acgacaagta gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca
    47161 ttgtttaaga accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct
    47221 tatctttctt ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt
    47281 gcattatatg gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct
    47341 ctgttggcat tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag
    47401 gaaactttgt gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt
    47461 ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa
    47521 aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca
    47581 cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg
    47641 cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg
    47701 gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg
    47761 caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg
    47821 cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg
    47881 ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg
    47941 ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc
    48001 cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc
    48061 caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag
    48121 ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca
    48181 gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc
    48241 cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg
    48301 gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa
    48361 atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg
    48421 tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac
    48481 tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag
    48541 gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta
    48601 ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct
    48661 ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca
    48721 aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag
    48781 aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt
    48841 cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct
    48901 ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag
    48961 aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa
    49021 aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg
    49081 cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac
    49141 agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa
    49201 aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa
    49261 aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc
    49321 atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt
    49381 aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa
    49441 ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga
    49501 acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg
    49561 atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt
    49621 taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac
    49681 atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag
    49741 aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc
    49801 ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat
    49861 aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt
    49921 attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt
    49981 ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta
    50041 ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc
    50101 tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac
    50161 aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag
    50221 ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg
    50281 ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac
    50341 ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg
    50401 acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt
    50461 taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg
    50521 tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa
    50581 cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg
    50641 ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat
    50701 tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat
    50761 ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct
    50821 acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc
    50881 aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta
    50941 ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac
    51001 tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca
    51061 agtgtctgtt tatccagtag ctcaagaagc ttaattgaat cttatgatta aagaggttgt
    51121 gacaagactt ttgtcacagc cttttttgtt tataaaaata aaagttgtag tggaggctac
    51181 gaagtttact ttgtcttttt acacttaata tttttttgcc aatttggaat caggatgttt
    51241 tgataactat cgttttcaat tgatggaagc tatattgttt gctatcagtg aaataattat
    51301 atggtaaaca gtgttctttt tcttgtttcc attataaaaa tcgtatcggg atttttcgtt
    51361 cttttaaatt gaacatttct atctatccgc gtgttatcaa caaaacaaaa tcaggtaaat
    51421 agttgacgaa acagaaataa atacatagta ttatgttttg aagcctaagt acaatagtct
    51481 atagaaaagg agcgatacca ttggcaaaac gcattgaccg aatttatatg tatgtcaaag
    51541 aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg agtaacaaca caggaaattg
    51601 ctgaagtgtt agggattcag cgaacaaata gcagtaaaga tctgaatcag ctagtgcgcg
    51661 aaggaaaact gataaagaca gacggacgac cagtacgtta catatatcaa acactcgtta
    51721 cgcacgtaaa gccagttaca aaacatgtag tcagttacaa agaaacgccg gtctctgaag
    51781 aaaaaaatac actccaaata gatactaagg atatctttgc aaaaatcatt ggagctaacg
    51841 gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat tttgtatccg ccaagaggat
    51901 taaattgttt gattacagga cctaccggat caggaaagac ttattttgca cacgcgatgt
    51961 atcattttgc aaaagcgcat catgtaatag atgaggaaac ggaactgatt gttttcaatt
    52021 gtgctgatta tgccaataac cctgagcttt tgatgagtca tttatttggg tacgcaaaag
    52081 gtgcttttac aggcgcagat gaagaaaaaa tggggattat cgatcaagcg gatggcggta
    52141 tgctgttttt ggatgagatt caccggttgc cgcctgaagg tcaagaaatg atcttttact
    52201 ttatggatca tggaacttac agtcgtcttg gggaaacaac gaaatcccat gaagcaaatg
    52261 tacggatcgt aggtgcgacc acagaagacc caggttcttc tctattagaa acttttgttc
    52321 ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa acgtcctgca aatgagaaag
    52381 tcgatttagt aaaaatcatg attgcccacg aagcaaaccg cacgcagcga aagatttctt
    52441 tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat ataggacaat
    52501 tgaagtcaaa tatccagtta gtatgtgcac gaggatttct aaatcatatg caatcacccg
    52561 aaatctcgat cacaatcgat gatttgacag aagggattcg tagcggattg attcaattag
    52621 caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag ataactgtag
    52681 caccaaatga tacgatcatg aaaatccaat cggactccta tgagttacct tataatctat
    52741 atgatatcat cggagataaa gcagctttac tgaagtcgga tggtttggat caagaagcaa
    52801 tcaatcattt catttctaca gatatcaaca tccatctgaa gtctttctat aaagaccatg
    52861 gtttttcttt taatgcagac agcaaattag cagaatttgt agatccgaaa atcatagaag
    52921 tgaccaatca gatttatgta atggtcaaaa gatctttacc atatgagttt cagcaaaact
    52981 ttatttatgc tatgagcttg catatcagtt cttttttgaa aaaaatcaac ttaggagaag
    53041 aacggcatac gaatgataac atccgtgaga tggcttttga ttttccagat gaatatgctg
    53101 ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca agtggagatt ccagaaagtg
    53161 aagactatta cttgaccgtt ctactcgtct cgttacgggc aaatcaagca agcggcagaa
    53221 tcggtgtagt cgttgcagct cacggaaaca gcacagcaag cagtatggtc caagttgtcc
    53281 aacagttgtt agatgcagag aacgtacgag cagtagacat gcctttggat atggatccga
    53341 aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt agatgaagga agcggggtca
    53401 tactcttagt agatatgggg tcccttgcgt cgtttaattc gcaaattcaa agagacactg
    53461 gaattccagt acgtactgtt gatatggtaa caacttctct agttttggaa actgtacgaa
    53521 aggtctcagt attagggaca gatctagata tgttgcatga atcattgaaa aattttcgag
    53581 gttacgcaga aatatcttct gtagaatctg ttcaggaagc aaaacctatt cataagaaaa
    53641 aagctgtttt ggctatctgc gcatctggcg aaggtacagc acaaagaatc aaagaattga
    53701 tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga agttctagct ttgtcgattg
    53761 tggaagtgaa agaagaactg ccagttatcc aaaaagaata tcagatcata gctactactg
    53821 ggattactga tttgaatatc caagccccat ttattccttt agaacgtttt attgatcaaa
    53881 atatcgaagt cattttggat cagttgctga tggaatcaga attggaggaa actgaattca
    53941 tctccctaga tgaagagtca gctaaaaata cgtgtgttga gtttatctct gataacttca
    54001 tctttattaa tggtagtaag ttaatcgatc caatgtggca attttctaca caaatctctc
    54061 agacgacggg aatcggcgat gaagaatatg gattcaaaat caacctagtg atgcacacag
    54121 caggaatgat cgaacgtatt attcggaatg aaccattgac tgtggaagaa aacgaattga
    54181 caaatacaac gaatgatcct ctttatagcc aattggcagc ctcagttgtt ctgttggaag
    54241 atcagataaa agtaaaagtg ccgatagaag aaatgtatta tctgctacgt ctagtccata
    54301 atcagttaga taaaaaagaa tacactgtgc catgaataca caaaaacgat acacttttat
    54361 agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat tcaattagtg tatcgtaaaa
    54421 aagttggcac gctttttgct tatagtagtg tgtgctagga ggtggttgtt cacacagaaa
    54481 tgataggggt ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga tattcgattg
    54541 gcacgtatag atgatcgttt aatccacggg caagtcgcaa cagtttggtc gaagcaaaca
    54601 ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc aagataaatt gagaaagttt
    54661 ctactaaaag aagctgcacc gccggggatc acagcaaatg taataaccgt gcagaaaatg
    54721 attcaaatct atcatagtgg attattatca cgagcaaaag tgatgctttt attcaccaat
    54781 ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt gaatatcggc
    54841 ggtatgagtt ttactgaagg caaatcaatg attaccaatt ttgtttcagt aaatcaacaa
    54901 gatattgatt cctttcgtta cttgaacagt caagaagtgg agttagagat tcgaaaagta
    54961 cctgctgaca ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa ggtgtagcaa
    55021 gaatggttgt tggaaaaggt caaataattc tgttgtaatg gttagcaatt attaatattt
    55081 ttgaatttgt tacaacaaaa gtaaatagga ggtataaata tggtagggat tatcctagca
    55141 agtcatggac aatttgctga aggaatctta caatccggtt caatgatctt tggcgaacaa
    55201 gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc cagatgactt gagagccaaa
    55261 ttggaagaag cggttgcttc ttttgataat caagacgaag tgttattctt agttgattta
    55321 tggggcggaa caccgttcaa tcaatcaaac acattattcg aagagcacaa agataaatgg
    55381 gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag cctacgcatc acgcttttca
    55441 atggaatctg ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga cggtgtaaaa
    55501 gtaaaaccgg aagaattaga accggcagaa gcaccgaaag cagcagttga agacgcacaa
    55561 cctaaaggag cacttccaga aggaactgtg gtcggagacg gaaaaatcaa atatgtatta
    55621 gcacgtgttg actcacgtct gttacatgga caagttgcga cagcttggac aaaagcagtg
    55681 caaccaaacc gtattatcgt tgtttcagat gcagtatcaa aggatgatct gcgtaagaga
    55741 ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg ttattcctat tagcaaaatg
    55801 atcgaagtag caaaagaccc tcgttttgga aataccaaag cattgctttt atttgaaaat
    55861 cctgaagacg tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt aaatgttggg
    55921 tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag tattgtctat gggacaagaa
    55981 gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt acgtaaagta
    56041 cctaatgatt cacgcgataa catggatgat attttgaaaa aagcaaaagc tgaattagca
    56101 aaagcgtaat aaataatatt aaaatttata ggaggcttat catgtctatt atttcaataa
    56161 ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat gaatttcaat
    56221 ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac ctcgaagctg
    56281 gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat atcggtgccg
    56341 ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta gttttaggtg
    56401 gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg gctgttgcag
    56461 ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat ttgatggatg
    56521 cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt gctgtatgtt
    56581 tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca gcacaaactg
    56641 tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct atcggtgggg
    56701 gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca aaagaagtat
    56761 ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca ttgattgctc
    56821 taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa atgggtggtt
    56881 catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt ttgaatgact
    56941 attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat caaaaaaaga
    57001 tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact acgaacgtat
    57061 gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat acaaaacaaa
    57121 agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac acccatacat
    57181 tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga atggcgcgcc
    57241 tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt tagctggtgt
    57301 tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag gtgcttcatt
    57361 agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga acattattcg
    57421 ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta aaattactga
    57481 tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt tagggatgtt
    57541 cgtattagct gcccttgtac aaagatgggt atcaatcaaa ttcttaccag ttgtatcaac
    57601 tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag gtggtgaagg
    57661 aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg aaaaggtaac
    57721 aacattacaa gacaacttag accaattgat tcctggttta gctgcattag gattaacatt
    57781 gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc ttggtttatt
    57841 cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa tatagctcgg
    57901 gcgttgtccc gagctttttt tatatagtat actgttcata aacaaatatc tggagggaga
    57961 aaatggttca atcaatcaat acaaaagtag acttggtaat tgatgctact tcgtttacgg
    58021 gtctgacaga ttacgggcaa atcatgatag gtgataatgg ttttgagttt taccatgcgc
    58081 gagatagacg caaatatatc caaattccat gggaagaagt ggattatgta ctagcatcag
    58141 tcatgctaaa gggaaaatgg attcctcgat atgccattca aacgaaaaaa aatggtacat
    58201 acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa tatgtagatg
    58261 aagatagaat ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt attcgtagta
    58321 tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag gaggttttct ttattttttt
    58381 actttgcaga agaaaatatg aaatagcaaa accaattatt tgtccccttt aaatcccata
    58441 tgctaaaatt gattagcaaa gaaggaggtt atattatgag ttggttatgg acattgattg
    58501 ttggagccgt gatcggagca attgcaggtg cgatcaccag taaaggaaat tcgatgggct
    58561 gtgtaacgaa tatcattgct ggactagtcg gttcaagtat tggacaagcg attttcggcg
    58621 aatggggacc aaaagctgca ggtatggcac ttattccatc tattttaggc gcagtgattt
    58681 tggtagcagt tgtctcattt ttctttggaa aacgatcata agtttttaca aaatagtaaa
    58741 ctaaaaggag ttgcagcgag actgtgactc ctttttggct ttatttattt aaaataaaaa
    58801 gaagttttcc ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg ctataatgga
    58861 aaaaagcgtg caaagaaaga gggagtttca ttatgatgct acgtgtagaa aaattgcgca
    58921 aaaaaatgca agaagaaaat ttggattcat ttttggttac cagtccgtac aatcttcgtt
    58981 acttaacgaa ctttacaggt acgacaggat tagctgttat tactttggaa aaagcatttt
    59041 ttattactga ttttcgttat accgagcagg cggcagcgca ggctcaaggg tttgaaatta
    59101 ttaaaaatgt cggacctatt tttgatgagg tagcaaactt agtacgcaaa gaagaactgc
    59161 actcattagc ttttgaggaa acaacagtct catttttaga atattcggtt ttagaagaaa
    59221 tcatcgatgc ggaattagta cctgtttcac agatgatcga agaactcaga gaagtgaaag
    59281 atgaagaaga aatcgctatt atcgaaaaag catgtcatat tgcggatatg gcatatgatc
    59341 acatcctgaa aatgatccaa ccgggcatga ctgagattga agtcgcaaat caactagact
    59401 tttatatgcg ttctcttggc gcttcaggtg tttcttttga tacgatcgtt gctagcggat
    59461 tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa ggtgacttga
    59521 tcacattgga ctttggttgc tattacgaag ggtacgtttc cgatatgaca cgtacattcg
    59581 ctattggtga tcctggggaa aaattaaaag aaatttatca aatagtctta gatgctcaat
    59641 tagcagttat tgatgctgca aaaccaggaa tgactggtgt ccaattagat gcagttgcta
    59701 gagattatat ttcaaaacat ggttacgggg aagcatttgg acattctaca ggacatggga
    59761 tcggtttaga gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag caatttgtac
    59821 caggaaatgt gatcactgat gaaccaggga tctacttacc aggaatcggt ggagtacgta
    59881 ttgaggatga cttgttgatc acagaagatg gaaatcgtgt gttgactcgt tcaccaaaag
    59941 aattgatcat cttatagttt catcaataga agaagtaagc gtaaaaatca gtgttcacag
    60001 actttgttat agaaaatcac tcgatttaat gatacactat agaagaactt ttaagctgta
    60061 agagataata aagttgggtg ttttctccaa ttcatagcag tttgcagctg atcggcggca
    60121 gttttcttac cacgtgatca ctatgccgca tcatagagaa ttcggatgta tctcgtgctt
    60181 aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat ttgattttaa acgcaaacca
    60241 agatttagga gagatcgtga tcgctcctga agtcatcgaa gtaattatcg ggattgcagc
    60301 ttctaaagta gaaggcgtat acggaatgcg cggaactttt gccagcaatg tcactgaatt
    60361 attaggacgt gcagctcacg gaaaaggtgt ttacttagca aacgaggaag atggcttgaa
    60421 agtagatctt tactgttatc tagaatatgg tgtttcagtt cctaaagtag caatggaaat
    60481 gcaggaacgg gtcagccagc aagtcctttt catgacggat atcgatttat ctgaagtcaa
    60541 tatccatgta gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg aagaactttt
    60601 tgaagacgaa gaggaagaga atgaataaag aattatcaag acacgagatt cgggaaatgg
    60661 cattgcaggc tttatttcca ttagacttca atgcagacct aacaaaagaa gatgcgattt
    60721 tcaacgcaat cgaattagac caccgcgaca tgatcaacga agatgaatca gagtttgttc
    60781 ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa ttggataaag
    60841 tgattgaaaa acatttaaaa aacaattggc acatcaatcg gatctcaaaa atggatttgg
    60901 tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac agatgtacca gcggctgttg
    60961 ctttaaatga agcaatcgag ttggcaaaaa catttagtga tgaccgttca cgtaaattcg
    61021 tcaacggtgt tttatccaat gtattgaaag aatta
//
LOCUS       DAFEPQ010000010        52206 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.010, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000010
VERSION     DAFEPQ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.010"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(24..722)
                     /locus_tag="LP543_000693"
     CDS             complement(24..722)
                     /locus_tag="LP543_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBL2320881.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="LP543_000694"
     CDS             complement(715..2067)
                     /locus_tag="LP543_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBL2320882.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="LP543_000695"
     CDS             complement(2290..3690)
                     /locus_tag="LP543_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBL2320883.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="LP543_000696"
     CDS             3851..4588
                     /locus_tag="LP543_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBL2320884.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="LP543_000697"
     CDS             4713..5192
                     /locus_tag="LP543_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBL2320885.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="LP543_000698"
     CDS             5189..5956
                     /locus_tag="LP543_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320886.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="LP543_000699"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="LP543_000699"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL2320887.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="LP543_000700"
     CDS             complement(6859..7461)
                     /locus_tag="LP543_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL2320888.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="LP543_000701"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="LP543_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="LP543_000702"
     CDS             8142..8726
                     /locus_tag="LP543_000702"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBL2320889.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="LP543_000703"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="LP543_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBL2320890.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="LP543_000704"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="LP543_000704"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBL2320891.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="LP543_000705"
     CDS             10627..10806
                     /locus_tag="LP543_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320892.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="LP543_000706"
     CDS             10828..11853
                     /locus_tag="LP543_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBL2320893.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="LP543_000707"
     CDS             12028..13272
                     /locus_tag="LP543_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBL2320894.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="LP543_000708"
     CDS             13425..14372
                     /locus_tag="LP543_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL2320895.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="LP543_000709"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="LP543_000709"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBL2320896.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="LP543_000710"
     CDS             complement(15274..15516)
                     /locus_tag="LP543_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320897.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="LP543_000711"
     tRNA            15637..15709
                     /locus_tag="LP543_000711"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
     gene            complement(15828..16466)
                     /locus_tag="LP543_000712"
     CDS             complement(15828..16466)
                     /locus_tag="LP543_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL2320898.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16589..17221)
                     /locus_tag="LP543_000713"
     CDS             complement(16589..17221)
                     /locus_tag="LP543_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBL2320899.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17572..17871
                     /locus_tag="LP543_000714"
     CDS             17572..17871
                     /locus_tag="LP543_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBL2320900.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            18366..20530
                     /gene="acm"
                     /locus_tag="LP543_000715"
                     /pseudo
     CDS             18366..20530
                     /gene="acm"
                     /locus_tag="LP543_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
     gene            complement(20802..21287)
                     /locus_tag="LP543_000716"
     CDS             complement(20802..21287)
                     /locus_tag="LP543_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBL2320901.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    21377..21722
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            21767..22408
                     /locus_tag="LP543_000717"
     CDS             21767..22408
                     /locus_tag="LP543_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL2320902.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            22427..23062
                     /locus_tag="LP543_000718"
     CDS             22427..23062
                     /locus_tag="LP543_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL2320903.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            23080..23895
                     /locus_tag="LP543_000719"
     CDS             23080..23895
                     /locus_tag="LP543_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL2320904.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            24214..25650
                     /locus_tag="LP543_000720"
     CDS             24214..25650
                     /locus_tag="LP543_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL2320905.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            25839..28286
                     /locus_tag="LP543_000721"
     CDS             25839..28286
                     /locus_tag="LP543_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBL2320906.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            28516..29583
                     /gene="rlmN"
                     /locus_tag="LP543_000722"
     CDS             28516..29583
                     /gene="rlmN"
                     /locus_tag="LP543_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBL2320907.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(29776..30879)
                     /locus_tag="LP543_000723"
     CDS             complement(29776..30879)
                     /locus_tag="LP543_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL2320908.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            31432..31980
                     /gene="aac(6')"
                     /locus_tag="LP543_000724"
     CDS             31432..31980
                     /gene="aac(6')"
                     /locus_tag="LP543_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBL2320909.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            32526..34187
                     /locus_tag="LP543_000725"
     CDS             32526..34187
                     /locus_tag="LP543_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL2320910.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(34257..34604)
                     /locus_tag="LP543_000726"
     CDS             complement(34257..34604)
                     /locus_tag="LP543_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBL2320911.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            34688..35629
                     /locus_tag="LP543_000727"
     CDS             34688..35629
                     /locus_tag="LP543_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2320912.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            35630..36673
                     /locus_tag="LP543_000728"
     CDS             35630..36673
                     /locus_tag="LP543_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2320913.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            36679..37749
                     /locus_tag="LP543_000729"
     CDS             36679..37749
                     /locus_tag="LP543_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2320914.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            37739..38692
                     /locus_tag="LP543_000730"
     CDS             37739..38692
                     /locus_tag="LP543_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2320915.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            38704..39714
                     /locus_tag="LP543_000731"
     CDS             38704..39714
                     /locus_tag="LP543_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL2320916.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(39999..40820)
                     /locus_tag="LP543_000732"
     CDS             complement(39999..40820)
                     /locus_tag="LP543_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL2320917.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(40917..41195)
                     /locus_tag="LP543_000733"
     CDS             complement(40917..41195)
                     /locus_tag="LP543_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320918.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            41463..43472
                     /gene="metG"
                     /locus_tag="LP543_000734"
     CDS             41463..43472
                     /gene="metG"
                     /locus_tag="LP543_000734"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBL2320919.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(43951..44538)
                     /locus_tag="LP543_000735"
     CDS             complement(43951..44538)
                     /locus_tag="LP543_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320920.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            44713..46035
                     /locus_tag="LP543_000736"
     CDS             44713..46035
                     /locus_tag="LP543_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2320921.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            46190..46966
                     /locus_tag="LP543_000737"
     CDS             46190..46966
                     /locus_tag="LP543_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBL2320922.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            46959..47528
                     /gene="rnmV"
                     /locus_tag="LP543_000738"
     CDS             46959..47528
                     /gene="rnmV"
                     /locus_tag="LP543_000738"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBL2320923.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            47521..48405
                     /gene="rsmA"
                     /locus_tag="LP543_000739"
     CDS             47521..48405
                     /gene="rsmA"
                     /locus_tag="LP543_000739"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBL2320924.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            48856..49485
                     /locus_tag="LP543_000740"
     CDS             48856..49485
                     /locus_tag="LP543_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320925.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(49609..50718)
                     /gene="ugpC"
                     /locus_tag="LP543_000741"
     CDS             complement(49609..50718)
                     /gene="ugpC"
                     /locus_tag="LP543_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBL2320926.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            50927..51349
                     /gene="mgsA"
                     /locus_tag="LP543_000742"
     CDS             50927..51349
                     /gene="mgsA"
                     /locus_tag="LP543_000742"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBL2320927.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            51672..>52206
                     /locus_tag="LP543_000743"
     CDS             51672..>52206
                     /locus_tag="LP543_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBL2320928.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT        17562 a         9074 c        10625 g        14945 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt ttaatccatt gatacatctg tctttttgtt taaaggaaat
    15781 aaagtatgct ttcttagcaa tagagcatgt attatttcta taaaaattta ggagaataaa
    15841 acgactagca atatagctgt gaaatcaaat atcacatgaa ctgcccaact tgtaatgatc
    15901 gagttgcttt taatggcagc ttggttgaat aacaatcttg ccaccccttg tatcaaaagg
    15961 atgtgtgcta cagtgtgtaa aatatttcca ttatcataag tactgtaatg gataaatcca
    16021 aagatgattg ctgacaagaa actagcgaca ggaatgctga acttactaga aaaaatagcg
    16081 agaaaataca ctgagaacaa ttcttctcca agtatcatga taggcaaaat aataaataat
    16141 aacgggctga aagcctcatt tattggattg cctgttaaac cccatttcaa aatttgttgc
    16201 aaaaataccg agactgcggc actgattact acattgataa aaaaatattt ggctatattt
    16261 ttgacaggag cgacaggttt cgagaacatt tttttcgtat cttttttacc aaaaacaagt
    16321 gtaaatgcta taaatccagt gatagttaaa gaaaaaatgc taataatgga tggcgcaaat
    16381 agcaacccta acaaaccgag tacaacgaca atagtgccca atatcccttt tttaagttct
    16441 attttttttc ctaaaatgtt attcatgata ataactccta ctattcagtt tttgaagata
    16501 gagtcagtat acatgaagag ttaaaatagg caatgttttt ttatggatat ttgtattgct
    16561 aaaaatttat cttatatatt cattttgatt atactacttt tatttgtaaa aattttctag
    16621 caaaaccaag tgcttgcaac tgattaagcg tacaattttt tagatgttcc gagcgtactt
    16681 caatcatttc aaaggagttg ctacttaaat cgatattttt taaatcagta tcaaaaaagc
    16741 tagcattttc taaatcatta gtagaaaaca ttattttagt gagggaacag tctacaaaat
    16801 ctgcgcttga taagacagag tgctcaaagg tactttcttt tatgtttgaa ccagataaat
    16861 ttacatagtc cattttacaa ttctcgaata acgtgttccc aatataactt tccatcaatt
    16921 tagtacctgt taatttacaa ttgacaaatt tcacgcgatg caacatgctt tttgtcaata
    16981 tacagcctga aaaatcacag ttttcgaata tacagtcggt aatctccatt ctttgaaaat
    17041 cattggaagc aaaaaatgca tacttgaaaa tagatttgta aataattgga tggcagatta
    17101 tttggttttc gatgacctct ttttgtgaaa ggatattata cagttcaaaa tcttcgtttt
    17161 catagatatg aaaaaaggat cctttattca agcttgcttc attaacttta ggaaaaatca
    17221 tatttaatta cctccatttt cataaagtca tattaacaaa agctaaacca acttacatta
    17281 gcagaaaatg agataggttt tcgccttttt cgataacgat agtgtcgcta tctcttgaaa
    17341 cgttcttaga attgccctga gagacacaaa ttaggcgtaa ttctaataaa aatcttagaa
    17401 taaaaatttt tactgattta atttagaatg tttttaaaat aaaaatcgtt gccataacag
    17461 tgtattgaaa ggttttaaca atccagtaat gatttaaaaa aatctagatg tagcgtatac
    17521 tttgtttata gaatgtagtg ggaagaaata tatttttggg aggaaatcgt gatgtctaaa
    17581 agtgaagaaa tagaaaacat ggtactaaaa ttatatgatc tcgttttaaa tccagagatc
    17641 aaagaaaagg aacgtatact tttgatagac gcaaagacag gcttagaaaa aggtcagtat
    17701 tatcctaaag tcataaataa tttggaacgt agtttacgtc cgttagcaat caggggagaa
    17761 ttgtctaaac cagtatcacc gttttatatg gagatatcaa caataggaaa atttgaaaaa
    17821 gaattaggtc gaggaatggc ttcggcaccg attacttttg ggcatttata aaaacgagtt
    17881 attcatctca atgaattaaa aagtatagtc aatctcataa tgcaggctag gttcatttat
    17941 ttgaacagag tctgtatttt tttattctaa ataaagaaac agatgtttct ttatattgta
    18001 ttttatcctt taattaacac aaaaacagct agtataaact aataatgtta attaaaagtg
    18061 tattttatga aactatcgtt atttattaat gttgcatttt tgtaagttaa aaattggaaa
    18121 tatagattga aaaaatgaaa gcaataagta aaataaaatg aatatatgcg aaaaaataat
    18181 ttacattagg aaaaaccttg taaaaacagt tttttggcat atttattttt tataaatatt
    18241 tgtaaaataa ctatcaattt tttagttaaa agattggaaa tgagtggggg aggaattgaa
    18301 gaaaggattt ttgagagatg atatagtagg ttttattttt agttaaaata ggggagatag
    18361 aaaaaatgaa aaagtgtaga aatattattt tatcaatgtt atttattatt actaatataa
    18421 catcactgat tcctgttcat gtctatgcgg atgcaggcag agatatcagc agtaatgtca
    18481 cttcgttgac cgtggaccca acgaatatta cagatggagg aaacataaaa gtaaaattct
    18541 cgtttgacga aaaaaacaaa atatccaacc aggtgattat ctttggatca attggccaag
    18601 tgaaggaaat attcgcggtg aaggattcca aaaagaaatc ccattaatga ttgaaaataa
    18661 aaatgttgga actttaacgg tcagaaaaga ttctgcgcag gttgtgttta atgaaaatat
    18721 taaaaaccta gactcagtag aaggttgggg gcaatttgag atacaggcca gaaacgtaac
    18781 cgataccggt gaagaaaata cgggaccatt caccgtgacg agcggtgata aaacagctac
    18841 tgtaaatgtg acaaagccag cttctggttc ttcttctagc gttttttatt ataaaacagg
    18901 agacatgcta cctgaagata ctaagcacat tcgatggttt ttgaatatta acaataacgg
    18961 cacttatgtt gaacaaccag ttaaaatcag tgatgaaatc caaagtggac aaagattaga
    19021 tccaagtacg tttgaaatta atcaaataca cttaggtgaa cagaaggttt atagaggaga
    19081 agagggaatc caacaatttt tacaagattt ccccagcgct acatttaatt tttcagtaac
    19141 tgataattac attgaaataa ctataccaaa aaactttgtc aatcttagga aaataatggt
    19201 gagttataag acgatcatag aaaacccaga acaaataaat tttgagaatc attcggaagc
    19261 ttggtttaaa gaatttaata aacctgctgt tgatggagaa agtttcaatc atacagttaa
    19321 aaatatttca gcttctggtg gagtcaacgg tacagttaga ggcgaattga agattttcaa
    19381 atatatcaat gacacagaga ttggtatccc aaatgtgact ttcgaattaa gacgagcaga
    19441 tgaacagcct attcaaggac agtctagtat cctgttgact tcgaatgaac agggagaagc
    19501 tagtatcaaa ggcctgcaag taggagacta tgttgtaaaa gaaaaagaag cacccaattg
    19561 gattgatttt gatccactaa gttcaaatga actcaaattc tccataaacg aaaatgatac
    19621 agagggagtc agtttaccta tttataacaa gaaaaaggtt acgaacatca cagcaactaa
    19681 aatttggaac ggtggaacca ctccaagacc gagtatttat tttaaattat tcagagcaag
    19741 tacaaacaat tgggaaccag ttcctgatgc agaaacaaaa cgattagaca atggaataac
    19801 ttcagtgact tgggaagata tccaacagta tgatgatagt ggaaacgaat atacgtttaa
    19861 agtgcaagaa gtagatcaaa acggaaatga ttatgtccct tcaggttatc aaaaaattga
    19921 aaacgggcta gtcgttacaa atgagaataa agaagtcatt tcagtctcag gacaaaaaac
    19981 atgggaagac aatgagaacc aagacggcaa acgaccgacg acgatcacag taaatctatt
    20041 agccgacggt caaccgatcc aacacaaaga agtaagtgaa aaagatgatt ggcgctatcg
    20101 tttcacaaat ctgccaaaat acagagacgg gcaagaaatc atctatactg taacggaaga
    20161 caacgtgccc gaatacagta cgacgattga cgggtataac atcaaaaaca gttatacacc
    20221 ggaaaaaaca aatatatcta taccaggtag ccatataaca ccgtggaaaa attttccaga
    20281 gaagacagag agagagaaaa ttagaaatct attctctcaa aaattgcaaa tatttgctca
    20341 tacaggagaa tccagcgaac aaagaagcaa ttatattagt aagagagctg tacctaaaca
    20401 gataacaaat aaatcaaaaa gcattcttcc taaaacaagt agtaaaaaat ctagttttgc
    20461 tgtaatcatt ggtttattga tagtaactat ctgtgctggg atattcttac aaaaatataa
    20521 aaaagtttag ttagaatata atattttttg ttaaatatga ctgaaatcaa aagaataaaa
    20581 ttccgattta taatcgaaaa aagaagcatt tttatatatg cttctttttt cgtagaatca
    20641 ataaatatgg atttggctaa ctatggaaga atactcgtat tagttttcag cgttcatact
    20701 agttgttaca ttgtttgatt ggccaaaaga atttctgata tattttgtgc taaataaaga
    20761 aaaagacgct tgaattggga aataagaaaa ctactctatc attacaaatt ttcataaact
    20821 ggcagatgtt ctaagtaaac aagtatcttt tcaaagtcgg aaaaatccat aatcaacgta
    20881 gtggtattga tatttggatg aaagcctacg gtatcttgac gatcaatagc agcatcgatc
    20941 acgacttgga tttttttctc tttgtcgtgc atcaatgcta atggagtcac tgtacctgct
    21001 ggaacgccta ggaggtcagg aagctgttct tcagaaacaa aagataagcg cttttctttt
    21061 aattgttcag ctagttgttt taaatcggcg tgcttttcat caggcaagat gacaagatag
    21121 atttgttttc cttttcttga tttcaataat agatttttta cttgcggacc aggtagggaa
    21181 aaagggacat ttttgactga cgtaatcgca ggatgatcga ctttttgaaa agagataccc
    21241 agttttttta gtagttcata agcttcttgt tcggttgcac gactcatcgc gcatcaccct
    21301 ccttttttta ttagtgtatc ataaaaaaga gttgacaaat gaaaaataat catgtaaatt
    21361 acacgtaaag ttaaaacaaa tgacatcgaa aagaagagta gccgttaggt gtttttagag
    21421 agtctctggt tggtggaaag aggcagcagt taaccgtgaa acacatcttg gagtcgattt
    21481 tctgaagcgt atagtaggaa aatccgggga gcccgttaca gctgcggtcg ttgctagatg
    21541 accagataag gctggctttt ccgtgaggag tcggtaaaaa aaggtggaac cacgtgatta
    21601 acgtcctttg atcttcggat caaaggactt tttgttttca tctgaacggc aacgactgtg
    21661 tgaggttttt tctgcaagga aaaaataaac agaggtggaa ccacgtataa tacgtcctct
    21721 tggcatttgc caagaggatt tttttatttt aaaggaggaa aagaagatgg agtatttgat
    21781 tgaagttatg ccgcaattat tagtaggagc actcacaaca ttgaaattat ttttatttac
    21841 cttgattggc tcattaccat tagggattgt tttagcatta ggattgtctt tgcattttaa
    21901 gccgctacgc tacttgcttc aaacatatgt atgggttatg cggggaacac cgctgttgtt
    21961 gcaactgatt tttatttttt atgggttgcc gacgattggg atcgtttttg atcgtttcga
    22021 agcagcactc attgccttta tcttaaacta tgcagcttat tttgcagaga tctttcgtgg
    22081 agggatacaa tcgattcctc aaggtcaata tgaagcagcg caagttttgc aattaagtcc
    22141 ctttcaaacg gtgacgaaaa tcgtcatccc acaagtgaca aagattgttt taccttctgt
    22201 agggaatgaa gttatcaact tggtcaaaga ttcttccttg atctatatat taggtttagg
    22261 tgacgtaatg cgtgctggga aaatcgcgat ggaacgcgac gcgactttac tgcctttgct
    22321 aggcgtggca atcatctatc ttacattgac tggtattcta acaatcataa tgaaacaagt
    22381 tgaaggaaaa ttaaattatt accgctaact gataggagga agaaatatga ttgaattgaa
    22441 gaaagtgaac aaagcatttg gtgaaaaaaa aataatcaaa gatttgaatc taatcattcc
    22501 agatgggaaa acattagcta tcgtaggtcc ttccggtggc ggtaaaacca cgcttttacg
    22561 tatcttggct ggcttagaaa gttctgacag cggagaattt tatttagacg gtgtaccgtt
    22621 caacccattc gagcttaaaa tgaaagaaca agttgtagga gtcgtattcc aagacttcca
    22681 gctatttcca catctgaata tttttgaaaa cattacctta gcaccaaaaa tggtattgaa
    22741 acagacaaaa gaagaataca ccaaaaaagc agaagaatta gcggcttcat tagggattag
    22801 tgatttattg aatatctatc cttatcagct ttctggcgga caaaaacagc gggtagcgat
    22861 tgccagagca ttggctatga atccacgggt attggcttat gatgaaccaa caagtgcttt
    22921 agatcctgaa ttacgccagc aagtcgagcg tttgatccta tcattgaaag aagatggggt
    22981 aacccaaatt attgtcaccc atgatttagt atttgcagaa aatgtcggtg atcaattact
    23041 aaaagtttcc ccaattcatt aagaaaggag aagatagaga tgaaaaaaac cagttttcta
    23101 ttcgctttga tcgcaggtct tcttcttttt gcaggctgtt cgaacaaaaa aacttcagca
    23161 gataattggg cgactatcaa cgaagagaaa aaaatcgtta taggtttaga cgatacattt
    23221 gttcctatgg gatttagaga taaagacggg aagctcacag gatttgatat tgatttagcc
    23281 aaagcagtct ttgaacaata tgggattacg gtagactttc agcccattga ctggtcaatg
    23341 aaggaatttg aactgaacaa tggaacgatc gatttgatat ggaacggcta ttccaaaacg
    23401 ccggcaagag aaaagaaagt gcaatttaca aaaccttata tggaaaacga tcaagtgctg
    23461 atcacaccta aagaatcagg aatcacaaac tttggacaaa tgaataataa gagattaggt
    23521 gcgcaaaatg ggtcatctgg ttatgatgta tttacgaaac aaccagaagt attaaaagag
    23581 atagtcaaaa accaagatgc tgttttgtat gatagtttta atgaagcatt gatcgatctg
    23641 aaaagcagac gggtcgatgg tctgctgatg gataaagtat acgcagacta ttatttgaaa
    23701 caaagaaacg aactagaaga cttcaacata acaaaaggtt cttatgagag tgaggatttt
    23761 gcagttggtg cacgcaagtc agatagtaca ttagtgaaaa aaatcaatga agccctagat
    23821 caactagaga aaacaggtga attccaaaaa atatccgata aatggtttgg cgaagatgtt
    23881 acgccgagaa attaacaaat aggggactgt gacaaaagac gtgtcacagt ccctttttcc
    23941 gaataaacag tgttctcctg ccaactcctt gtgactcgga gctgaatggc aggctcacaa
    24001 cctcttggta aacggtgttc aaaagccagt ttttcggtat taagccaaat ttatgcaaaa
    24061 atgtagaaag ccattttcac aaaatttttc ttactactcg aaactaagcg actttttcac
    24121 aacctccttt acataatttt attttgttgt ggaaggggtt tcgtgttatt ctaaaagagg
    24181 aacgattgaa ggttatagga aaggaagaga tgaatggaac acaaagaact tacctcattt
    24241 ccaaaagatt ttctgtgggg ctctgcttct gcagcgtatc aagtagaagg agcttggcaa
    24301 gaagatggga aaggagaatc agtctgggat cgatttgtac ggatacctgg aaagacattt
    24361 aaaggaacga acggtgattt ggcagtagac cactatcatc gttataaaga agatattgct
    24421 ttaatgaaag aacaaggtct gaaagcttac cgcttctctg tagcttggac acggattttt
    24481 cctaatggac gaggagaagt caatcaagct gggctcgctt tttatgagag attgatcgac
    24541 gaattgattg aaaacgatat tgaacctatg ttgactttat atcattggga tcttccacaa
    24601 gcattgcaag atgaatataa tggttgggaa tcacgtcaga tcattgaaga tttcactcat
    24661 tatgcagaaa cattatttga ggcattccgc gggaaagtcc attactggat tagtttgaat
    24721 gaacaaaata tcttcacttc attaggttat ttgttagcag cacatccgcc aggtgtgaca
    24781 gacccgaaac gaatgtatga agttaaccat attgctaatt tagcaaatgc atctgtcatc
    24841 aataaatttc acgagttaga aatgcctgga aaaatcggac caagctttgc gtatacgcca
    24901 aattatccaa ttgacagtaa tcctgaaaat gttctggctg ctgaaaatgc agaagacctg
    24961 atggccaatt attggatgga tgtctatatg tggggaaaat atccgattgc tgccatgagg
    25021 ttcttagaag aaaaaggatg ggcaccaacg attgaagctg gcgatgcaga attgctagaa
    25081 tctgctaaac cagattttct tgggatcaat tactatcaaa cggcgacgaa tgcattcaat
    25141 ccattaaacg gagttggtgc tggaaaaatg aatacgacag gaaaaaaagg ttcatcagaa
    25201 gaaactggag ttcctggaat gtataaaaaa gttgagaatc catttgtcga acgaacaaat
    25261 tgggactggg agattgaccc agaaggatta aggatcggac tgcgtcgtat cacaagccgt
    25321 taccgcatgc cggtactgat tacggaaaat ggcttaggag agtatgacaa gttgacagaa
    25381 gataaacaaa tccatgacga ctatcgaatc aattacttac aaagccacgt caaagccatc
    25441 aaagaagcaa tttcggatgg agcagaagtc ctaggctatt gcacctggtc ttatacagat
    25501 ttgcttagct ggctgaacgg ttatcaaaag cgatatggat ttgtatatgt tgatcaagac
    25561 gaaacacaag aaggatcttt ggaacgatat aagaaagaca gttattactg gtaccaaaaa
    25621 ctgattgaag aaaatggaga gaacctttag ttcacatact taaaacaaga aggtacggct
    25681 ggatatggcc gtgccttttc ttgataaaat catttagaaa ttaagaaaaa ctgtaaaata
    25741 agctgaaaaa tgaaagaaag cgtcagttct ttcaaacaaa actttagttc ccttactttt
    25801 ttttgtataa tgaaacttga tgaaatgagg gataataaat ggcacaaaag aaacggccta
    25861 ctaaaaaaag gaaaaagacg aaaaaaacga aaaagcaaaa acagcagcag gaacaaatgc
    25921 tgacgattgg tgttgggttt gccttgattt tatttgctgt cttcggcttt ttaaaattag
    25981 gttttttagg aatattgatt gcaaacggct ttcggatcat tgcaggaaat acttaccaaa
    26041 tcctctgtct tttattagcc gtattaggat tttggattgt cattaagaat acagagtttt
    26101 caattggtaa aaaccgacgc tggtttggcg gcatactctt ttatttcggt atcttattat
    26161 tgttacatgc ccatttattt ggcaaactcc atactgggga accaaatatc atggggacga
    26221 catgggattt gttagccagt gatataaaac aaagtcaagt agacaataac gttggtggtg
    26281 ggatgattgg agcaatcctt tatcatttta cttacttctt gattgcacag cctggaagtt
    26341 atttagcagc agtgctatta ctggcaggag gagcattcct gttcagtaat ctggaaggct
    26401 atcagctgtt aaatggtatc caatcaattg gagaacgagt acaggaatta ttggaaggtg
    26461 atcctgccaa gcaagctcga aagcaagcgg caaaggaaga gagaatgaaa cagcgcgcag
    26521 aagcgaaaga agcaaggcgc ttagctgcaa aagaagctgc agaaaaagaa gctgcagaat
    26581 atgagaagaa aaaagcggtg agccagcaaa gggaaaatac tgctgatgaa tggcaggaac
    26641 cagaaaatac agagccggag cagctaagtt ttgttccaat cgacagtttt caagaaaata
    26701 tccatcctgc gaatctagag aaaccagtac cagatacacc aaaacagacc aatacagcag
    26761 aaggtttttc agatgagttg ccagaggatg acggtacctc attagaattt gagattgaag
    26821 cagaacagga aaatcaagac tatgaattac cgacagttga tttgcttgat tctataccaa
    26881 ctgtggatca aagtgatgag tataaaaaaa ttgaaaaaaa tatcggcgta ttagaacaaa
    26941 ctttccaaag tttcggtgtc gatgccaagg tcgtcaaagc cagcttagga ccatccgtca
    27001 caaaattcga agtccagccg gctgtagggg taaaagtaag taaaattgtg aacttaacag
    27061 atgatatcgc gcttgcttta gcggcgaaag atgtccggat ggaagcaccg attccaggta
    27121 aatcactgat tggtattgaa gtaccaaatg gaaagatcag tatggtttcc tttagagaaa
    27181 tcatcgaagc acaaccaaac cacccagata aattactaga agtaccttta ggacgtgatg
    27241 tttctggacg ggtacaaacg gctgatcttt cgaagatgcc tcacttactc gtggctggtt
    27301 cgactggtag tgggaagtca gtagcaatta atgggattat cacaagtata ttaatgcgtg
    27361 ctaagcccca tgaagtaaaa ctgatgatga tcgatccgaa aatggttgaa ttgaatatgt
    27421 acaacggcat acctcatctg ttgacgccgg tcgtgacgaa ccctagaaaa gcagcgcaag
    27481 ccttacaaaa agtagtacaa gaaatggaag aacgatatga aaaattcgcg gcaaccggag
    27541 taagaaatat ttctggttac aatgaatttg ttcaacagaa aaacttggaa aacggaacga
    27601 aacatccaac cttaccattc atcgtagtga tcgtagatga attagctgat ttgatgatgg
    27661 tagccagcaa tgaagttgaa gatgcgatta ttcgtttagc gcaaatggct cgggcagcgg
    27721 ggattcatat gatcctagct actcaacgtc caagtgtgga tgttatcaca ggaatcatca
    27781 aagcaaatgt accttccaga atggcatttg cggtatcaag cggtacagat tctcggacga
    27841 tcattgattc taatggggca gaaaagctct tagggcgagg agatatgcta ttcttgccaa
    27901 tgggtgagaa taaaccgatc cgtgtgcaag gagcattcat ttctgaccac gaagtagaac
    27961 gggtcgttca atttgttaca gatcagcaag aagctcatta tgaagagaaa atgatgccga
    28021 cagatgaagt tgaaacgaca ggtgctccag aacagccaca agatgagctg tttgaagaag
    28081 caaaagcact tgtcgttgaa atgcaaacgg caagtatttc ccttctccaa agaagatttc
    28141 ggatcggtta caaccgagca gcacgtttag tagatgaact ggaagctcat ggagttgttg
    28201 gcccgtcaga aggaagcaag cctcggaaag tattcattga gcaggaagaa acatcagaaa
    28261 cgttgccgga cgataatgac atgtaaaact tgtgtgatga agggttgaaa aacgaataac
    28321 caataaaaag acaaggcatg atgataccaa aagaaaaaat ggtttttttc catcttttct
    28381 aatacggtat gatgtcttgt ctttttagtt tttgtgagat taaagttcta ttcgatttat
    28441 ttgtgaagta ttgcattaag gaaatgaatc atgtatagta tgtagcgaaa ctttgagaaa
    28501 aagagggaaa atactttgga aaaacagtcc atttatggct tgacgaatga agaattaatc
    28561 aattggttta tagaaaacgg cgagaaaaaa ttccgagctg cccaagtttg ggaatggttg
    28621 tatcaaaaac gtgtaagtaa tttcacagaa atgacgaatc tatcgaaaca attgatcgaa
    28681 aaattatcgg agcattttat catcaatcct ctaaaacaga tggtcgtcca agaagcttcg
    28741 gatggtacag taaaatattt gtttgaactc cctgataaaa acatgattga gactgtactg
    28801 atgagacaag aatatggctt gtcagtttgt gtgacgacac aagtaggatg taatatcggc
    28861 tgtacgtttt gtgcaagcgg gctgctcaaa aagaaccgtg atctgactgc tggagaaatt
    28921 gttgcacaga tcatgatggt ccaacattat tttgatgagc gcaatcttgg cgaacgcgtt
    28981 tcacatgtcg ttgtaatggg gatcggtgag ccatttgata attacgacaa tgtaatggac
    29041 tttttgcata tcatcaatga tgccaaagga ttggcaattg gtgctcggca cattacagtt
    29101 tccactagtg gattagcaca caaaatcaag gaattcgcta ataacggctt gcaagtcaat
    29161 ctggctattt ccttgcacgc gccgaataat gaggtgcgaa catcgatcat gcggatcaac
    29221 cgaagtttcc caattgagaa attgatggaa gctgtggatg agtatttaga gaaaacaaac
    29281 cgtcgaatca cgtttgaata tatcatgttg aatcaagtaa atgatagacc agagcatgcg
    29341 caacaattag cagatctatt aaaagataag aaaaaattag catatgtcaa cttgattcca
    29401 tataatccag tcagtgaaca tgatcaatat tctcgcagtc caaaagctga tgtattgaga
    29461 ttttatgatg tattgaagaa aaacggcgta aactgtgtga tacgtaaaga acatggtaca
    29521 gatattgatg cagcttgcgg acaattgcga agcaagcaaa tgaaaaaaac agttgccaaa
    29581 taatcgtatc ctactcgttt tttcaattac cataaaagga gcagttttct taattaagga
    29641 actgttcaac ttaaataaga gaaaagattc tatagcgaaa gctataaagc tttttttgac
    29701 aagaaaaacc ctcatcaagc aaagaaatgt cctcaatttg atgacagcct gcttgatggg
    29761 ggttttagca attatttaac tgattttctt atatccttgc tggctttctt gtgtagcatg
    29821 ataagtttct acgtcaagaa tggcctgttt aaatttttct cgggtgattt cataaggcac
    29881 atgtttaata tcatttgttg ccatggcttt atctaagatt gcttctaaat cggtcgtgac
    29941 attgagattc atgtcggtta acgatactgg aagttggttc tttttcataa acacatagat
    30001 tttatcaaac aaatcgaact gctgatccat catcaataag gtcagtacac cataactgac
    30061 agatgctccg tgaaggtatc cctccatttt ttctagaaca gtcgtaccgt aatagaatga
    30121 gtgtgccata gcagaattat agttattgtt aactaaaaca gaaaccaatc cggtagtaac
    30181 aatgatatcc gaaacgactt gttcaattgc atatgaggga cgatcgtcgg cactatcttt
    30241 caatgcttgc gtaccataag tcaagagacg atcaatgcac atttttccga tttggattcc
    30301 taatctatcc gtgtagccta gatcatcgcc acgagcagaa aaagtagatt cgacttcttt
    30361 ggataaagca tcgccaatac ctgcccataa gtatttatca ggtgcttcgg caataattgc
    30421 tgtatcaatg aaggtgtgta caggtggttt tgaaggaaag aagagtccgt ccattacgcc
    30481 ttcttcgtta tagatgacac ataccgcagt gacgggcgaa cagttcgaac caatagtggg
    30541 aaaggtgaag actggtttag ctagtttttc accaatgact tttacagtat cgcaagcacg
    30601 gccaccaccg actgcaaaca ggacgtctgc tttttggaca ctttcatgct ggatcaactg
    30661 ttcaatattt ccataagttg aattaccccc gtaccagaga ctggcgacaa cctctatttc
    30721 ctgtgtcaaa gctactttta ttttgttttc tgcctttgaa agtgcagtct tccctccaat
    30781 cagcactact tttgtcccat attctttgac gattccattt gctttttcat acgcatctgt
    30841 tccaatggta taatttggta agaaaacact ttgactcata ttcatttcct tctttcttcg
    30901 ttgaaacggg ttaaaatttt aaagagtgat cgatctgact gcggctgtag atggagtaga
    30961 ggtgaaccta agacaggata agtgatgaat aaggtggcat gaacaccttg aaatcatcaa
    31021 taagtatcct aaaaacgaac aagtacaaaa acattgaaaa cgacaataat gaatttttgt
    31081 tcctcttagt atttctaccg attccctttg cttttttgta attatctaaa aattctaaaa
    31141 attaagatta ttatatgaaa acaggtttta tttgtcaatt cataattggt tttttttaaa
    31201 gcaaaagata atctatatca tgatgaacaa acagagtttt ttcctccaat tgatggcttt
    31261 cctgatcttt cctattttga ataaagtgtt tctgtataat ttttttattt tataagtaag
    31321 ataaataatg aaagaataga gatactgatt ggtagctaaa gatttttgag cgagaacatt
    31381 tcgttttttc aaaaggactg ttagaataag caagaacaag ggggaagacg tatgataatc
    31441 agtgaatttg accgtaataa tccagtattg aaagatcagc tttctgattt actgagactg
    31501 acttggccgg aagaatatgg agacagctcg gcagaagaag tagaagaaat gatgaatcca
    31561 gaacgaatcg cggtagcagc ggtagaccaa gatgagttag taggatttat tggtgcaatc
    31621 cctcaatacg gtatcacagg ttgggaattg catccattag ttgtagaaag ctcccgacga
    31681 aagaaccaaa taggtactcg attagtcaat tacttagaaa aagaagtagc ttccagagga
    31741 ggaatcacga tttatttagg tacggatgat ttagaccatg gaacaacgtt aagtcaaacg
    31801 gacctgtatg agcatacatt tgataaagtg gcttccatcc agaaccttcg tgaacatccg
    31861 tatgaattct atgaaaaatt aggttataaa atcgtaggtg tcttaccaaa tgcaaatggc
    31921 tgggacaaac cggatatttg gatggcaaaa acgattattc ctcgaccaaa ttctcaataa
    31981 taaaaagagg ttgtgacaga agtaatcagc ttcaagaaat aagaaggaat ttacgaaaat
    32041 tacttcttga atttttgtga aattccaact tatttccgaa gaagctactt ttggaacacc
    32101 gtgtaccaga ggttgtgaaa aagtcgctta gtttcgagta gtaagaaaaa ttttgtgaaa
    32161 atggctttct acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
    32221 cgtttattca gataagggac tgtgacaaga cttttgtcac agtttctttt tgtttattct
    32281 tttttatctt tgtatggcaa taatagacaa tataacgaaa attatttaat ggcttttgga
    32341 agaccatgga aaaccttgta caaagctatt ttttctgaaa aattccttga aacctcacga
    32401 aaaaatcatt attttatgaa aaagacaaaa aaaagtgttg attttttaat aattattcat
    32461 taaactctta aacaacttca gaaaatatta attattctga aaattccaat aaaggggtac
    32521 gtaaaatgaa aaagaaattt acatttggtt tggttgcggt atgcggcctt gtacttgctg
    32581 gatgttatgg cggcagttca gccacaagca gtagttcagc aaaaagcggt tcagcagatg
    32641 gaggaggcgt atttaactta gtcgtgcctc aagaaatgcc tacagctgac ttatcagttg
    32701 caacagatac aatcagtttt acagcattga ataacgttta tgaaggaatt taccgtcttg
    32761 atgaagacag caaaccacag ccggcaggag ctagcgagtt ggcagaagtc agtgaagacg
    32821 gattaactta caaaatcaaa ttacgagaag acgcaaaatg gtcaaatggt gatcctgtta
    32881 cagcagcgga ttacgtttat ggctggcaac gaacagtaga cccagctaca gcttctgaat
    32941 atgcttacct gtatgcgcca gttgaaaatg cagaagacat tacagctgga aagaaagata
    33001 aatcagaact agggatcaaa gcagttggtg actatgaatt agaaatcaaa ttgacgaaac
    33061 aaacaccata tttccaatat ctgcttgctt tcccatcatt tttcccacaa aaacaatccg
    33121 tagtggaaga aaatggggat gcctatgcat ctgcaagtga taaagctgtt tataatggac
    33181 cattcacatt agaagatttt gatggaccag gaacagatac agaatggact tataagaaaa
    33241 atgatcatta ctgggacaaa gatacagtaa aactttctga aatcaaagtc agtgtagtaa
    33301 aagaatcttc tactgcattg aacttattca aagatggcca agcagatgac gtgatcttgt
    33361 ctggtgagtt agcacagcaa aacgcaaacg atccagctta tacttctgtt aaagaagcgc
    33421 ggacaagcta tatcgaattc aatcaacgtg aaaagaattc accattcaac aatgtgaacc
    33481 taagaaaagc tatttcttat tctatcgatc gtgaagcttt agtaaaacaa gtactaggtg
    33541 acggatcagt tgtctctaca ggattgatcc catcagatat ggtaaaaagt ccaacaacga
    33601 acgaagactt tgcaaaagaa gctggaaaat tagtttctta tgacaaagat aaagcaaaag
    33661 aatattggga aaaagcgaaa aaagaattgg gcgttgattc tttagaattt gatttgatgg
    33721 cttctgatga tgattcatcg aaaaaagtaa tcgaatatgt tcaaaactca atccaagaaa
    33781 acttggatgg cgtaacagtc aaaccaacac ctgttccatt ctctgtacgt cttgaccgct
    33841 caacttcggg tgattttgac gcagtactag gcggatgggc tgctgactat gcagacccaa
    33901 gcagctttac tgatttgttt gtcacaggta attcatacaa ccgtggtcgc tggagcaatg
    33961 cagaatatga cgaagcagtt aaagcagctt catcaacaga cgctggcgat gaacaagccc
    34021 gttgggatga tttccaaaaa gcaatcaaga tcatctctga tgatatgggt gtcgttccgg
    34081 tgtaccaaaa agcagaaggt cacctagtga atgaaaaaat caaaggtatc gttcaccatg
    34141 cggcaggcgc ttcatgggat tacaaatgga catacgttga agaataatcg ttaataccgt
    34201 gagcatgaga aaaaacgagg ctgcggcaaa tgtcgcaacc tcgttctcat attcacttat
    34261 cctaacaaag aaaaaagcat gaaaatagca gaaagagcaa ttagtattcc ggcaatgatc
    34321 agccaaaagc tgtacatacc gtagctggca gacgagagcg aggaaaattc tggttttttc
    34381 tttcggttcc ttgttcgtgc aagatggacc gatcgctgaa aatgatcgaa gaaacccgaa
    34441 gaaaagaccc ataaaaaacc gccgataatc agaaacggca atgaccataa gaatagatca
    34501 ttagataatt gaatgagtgt aaaggaatgg ttgatgagat tttttattaa aataaccaga
    34561 atcagtagaa aagctactgc gtaggggata gatttttttt tcatttgatt gctcctttat
    34621 ttttggttca ttatctaata atgtagcgaa acatctgtca gaagtcaaaa gctaggggga
    34681 aacattagtg aatagttata tcaaatatgt attaaaacgt gtctttttca tgatcatcac
    34741 gctttggtta attgcaacaa tcaccttttt cttgatgcaa ttgctgccgg gcacgccata
    34801 tacgaatcag gaacgtttga gtcctgaaac aatcgagatg ctaaataaac aagtcggttt
    34861 ggataaacca gttatcgtac aatatgggat ttatctttct aatttgcttc aaggagattt
    34921 tggaatttcc ttccaattta aaaaccagcc agtcgcacat ttgttagctg gaagaatcgg
    34981 accatccttg cagttagggt tacaggcaat tatctttgga acagtcctag ggacgatttt
    35041 aggaacgatc tctgccatga agcaaaatac atgggcagat acatcttcta cactcgtagc
    35101 gatattaggt cgttccattc caaactttgt atttgctgtt ttattacaat atatctttgc
    35161 aatcaaatta caagttttgc caatcgcaaa atgggatggc tttgtgtaca caatcttacc
    35221 aacaattgcg ttggccatgt cgccgcttgc tgactctgct cggtttatcc gtacggaaat
    35281 ggtggaagta ctgcatagtg attatgtgga attagcaaaa gcaaaaggat tgagccgttg
    35341 ggaaatcgcc tttaaacatg gtcttcgaaa tagtttgatt ccattgatga ctttattagg
    35401 accgttagct gtagcattga tgaccggatc actggtggta gaaaatatct ttgctattcc
    35461 tggtatcggg gaacaattcg ttaagtcaat cacaacaaat gattatccta cgatcatggc
    35521 tgtaacgatc ctttactcat ttatgctgat ttttgttatt ttgatcgtgg atctattgta
    35581 cggtctagtt gatccaagaa ttcgtgtttc agaagggagc cgaggataaa tggaaatgat
    35641 accaaagcgt tatgaaacag ttgcagatat ccctgcggat gaatttcttc cattacaaaa
    35701 aaataccgaa gaagaacgtg aacagattac tgcaccttct ttgaatttta tccaagattc
    35761 atggcgccgt ttaaagaaaa acaaagctgc cgttatttca atggttttac tattggtcat
    35821 cattttgatc tctgtgatca caatctttgt ttctcctcat gatccaactg cccaaaacgt
    35881 ggcttatatc aatttgcctc ctcgtattcc gggaatcaat atcgatggat tgaatggaaa
    35941 agcaatggtt ggtggtgaac ttgttgataa atatgctcaa gcaaacgtcc cttcaaacgt
    36001 aaatttcttt ttaggaacag acggtttggg acgtgatgta ttaagccgct tatttatggg
    36061 aatgagaatc tcgttgctga tcgcatttat tgctgcatta ctagatgtaa cgattggtgt
    36121 agcttacggg ttggtttctg gtttgttagg cggacgtgtc gataacgtga tgcaacgttt
    36181 tttggaaatc ttatcaggaa ttccaaacct cgttgtcatg attttgatgt tggtcgtttt
    36241 tgaaccagga attttttcaa tcgtggctgc tatggccatc acaaactgga taccaatggc
    36301 acgtatcgtt cgtgcacaaa caatgaaatt aaaagatcaa gaatatgttt tagctggatt
    36361 gacattaggt gaatcaagaa tgaaaattgc attcaaacat attttgccga atatctctag
    36421 tgtcattatt attcaaatga tgtttagtat tccatctgct attttcttcg aagccttttt
    36481 aagctttatc ggtttaggtc ttacgcctcc gtcagcttca ttaggaacga tgttgagcga
    36541 agggtacaag acattcctgt atctgcctta tctattatgg attccggcag taactttatc
    36601 ggttatcatg attggcttta acttacttgc tgatggattg cgtgatgcct ttgatcctaa
    36661 aatgaaagag tgaagacgat gacgaaaatt ttagaagtga aagacttaga aatctctttt
    36721 gatacgtatg ctggaaaggt acgagcgatt cgcggtgtca attttcattt gaataaagga
    36781 gaaacattgg cgatcgttgg ggaatctgga agtggaaaat cagtgacgac cagaagcatc
    36841 atgcgcttgc tgtcaagcaa cgccaatata gatgctggtc aaatcctctt taaaggtaac
    36901 gatatcgttg gaaaatcaga aagagagatg caaaaaatcc gtggtaaaga aatagcgatg
    36961 attttccaag atccaatgac ctctttagat ccgacgatgc ccattgggaa gcaagtcgct
    37021 gagtccctta gaaagcataa taaagtttca aaaaaagagg gacaaaaagc tgcattagaa
    37081 ttgcttaaat tagttggaat acccgaagct gagaaacgta tcaacagtta tccgcatcag
    37141 ttctcaggag gtcagcgtca acggatcgtg atcgcgattg cactggtatg ttatccggaa
    37201 atcctgattg ctgatgaacc tacaacagcg cttgatgtga ctattcaagc acagattttg
    37261 gagctgctga aagaaattca gacaaaaatc gatacatcaa ttatctttat cactcatgac
    37321 cttggtgtag tagcgaatgt cgctgatcgt gtcgctgtca tgtatggagg aagaatcgtt
    37381 gaagttggaa catcggaaga aattttctac aacccgcaac atccctatac ttggggactg
    37441 cttggttcaa tgccgacatt agatagtgcg aacgatcgat tgtatgctat tcctggttct
    37501 ccaccggatt tattgaaccc gccaaaagga gacgcattct atccaagaaa tgaattcgcg
    37561 atgaaaatcg atgcagaaca agaaccacca ttttttgaac tatcaaaaac acacaaggca
    37621 gcaacttggc tacttgctcc gcaggcaccc aaagtgacac cgccagaaga aatccaacgt
    37681 cgctgggcta tctttaaaga gaaacaaaaa catacttacg gtcaaggagg aattgctgat
    37741 gccaaataaa ctacttgaag ttaaaggatt gaaacagtat ttcaatgtag gaaaaaagaa
    37801 tgaagtacat gctgtaaatg acatcagctt tcacatttat gaaggagaaa cattcggttt
    37861 agttggagaa tctggaagcg gaaaatccac tactggccgt acgatcattc gcttgaatga
    37921 acctactgat ggtgagatat tattcgacgg tcaagatata acgaagatca aagataaaaa
    37981 aggtttgacg aaattccggc atgatgttca gatgatcttt caagatccat acgcttcgtt
    38041 gaatcctcgt atgaaagtca gagatatcat tgccgaagga attgatgtta atggactagc
    38101 taaatcatct gaagagcgtg caaaaaaagt cgatgatcta ttgagaactg ttggattgaa
    38161 tccaagccac ggtactcgtt atcctcatga attttcaggt gggcaacgcc agcgtattgg
    38221 tattgcacga gcattggcag taaaaccgcg ttttatcatt tgtgatgaac caatttctgc
    38281 actagatgtg tcgatccaag cccaagtggt caatttgttg caagatctcc aaaaagaaca
    38341 tcaattgacc tatctattta ttgcacatga tttgtcgatg gttaaacata ttagtgaccg
    38401 aatcggtgta atgcataacg gcttgttatt agagatggga acaagtgatg aaatctataa
    38461 tcacggtgtt catccttata cagaaagttt gttatctgct attcctctac cagatccaga
    38521 ccatgagaga aaacgcagaa gaatcaaata tcaacctgaa ccagatgacg gacaagtacg
    38581 tcaacttcgt gaaatcgcac cagaacattt tgtctatgca actgagcagg aagtcccata
    38641 ttacgcaaaa aaactaaaac gacaaaagga atctcttctg gttgctaact aggagggaca
    38701 tagatgctaa aagcttttaa attccgtatg tatccgacag aagaacaaaa acagcaattg
    38761 attcgtactt ttggatgtgc tcgatttacc tataaccatt tgctgaaaaa gcggcagaaa
    38821 agttggcaac aaacaggagt agccaatttt tcgctcacac ccgctacatt aaaaaaagaa
    38881 tacccattct taaaagaagt agacagtctt gctttagcaa atgcacagct taatttggat
    38941 cgggcttttc gcaattattt caagggacgt gccagcttcc caaagcttaa aacgaaaaaa
    39001 agtatgtggc aatcttatac gaccaacaac caaacccgta cgatctatct ggaaaatggc
    39061 tacttaaagt taccaaaaca aaaagaacta atcaagataa atcagcatcg gccagttgaa
    39121 ggatcgattc gctccgctac gatttctgct cgatataatg aagaatttta tgtcgcacta
    39181 ctttgtgatg tttcgccagt aaaaaaagaa tctttagcta aatggatcgg gatcgcctat
    39241 catccgaaga cattgatcca aacatctcga cctctagaag taactttacc aaaatttcac
    39301 cagacagaag aaaagctgca acatgcacag cggaaattga atgtgaaagt ccgctccgct
    39361 caccatagaa agatcaggtt agatcaggca agcaattatc agaaacaaaa gagaaaagta
    39421 atggatcttt atctgaaaca aaagaaccag cgagaagatt atcttgaaca attatctggg
    39481 aaattggtca aacaatatga ctacttattt gtagaatctt ttccaaaaga agaggcacat
    39541 gctgattttt ctattcacga ctggcataaa ttaataacaa aattgcgtta taagtcccaa
    39601 tggtataata aaaaatttct tttgatcaat accgacggag cagaagaaag caattccgta
    39661 agaaagagcc aagtagtaga aaaaatgggc agacattcgg ttatcaaggg ataaaaaatg
    39721 aatgaaactg ataggagcgt tagggttagc cttgttaata aaaagtctta atgacttttg
    39781 tttctaggaa tttttctttt gaagaaaagc tcactaatta tgacaaacca ataaaacaat
    39841 agcagaaaaa gttcgtacga gctttttctg ctattgttaa ttttttattc agaaagttta
    39901 cgaatacatc aaaattctga aacaaaaaac gtaactcaca aagagttgtt tttgcgtttt
    39961 ttcttgaatg attaggtctg aatcttttgc tcacaacctt attcatggtg ccccggcttg
    40021 agctgataga tttttgaagg acggccgatt cctacaggac gttcaccgat ctcatcgaaa
    40081 tattggagca tagctttctt aaaattggaa tgatcgatcg ttttataatc gatccctaag
    40141 aatttagcaa atacttttcg agcttccgta atcgtgaaat ctttacctaa aacttgtaga
    40201 acttgcggct cgtgttccat tttattgact acacggttga atgctttcaa gatgatttga
    40261 ctatggtcaa aagccaaagt atctttgcca gaagactctc cagtgactaa atcaagcgag
    40321 atagccactt ctccgctaga taaatggatc ttgttactat gacgttctag tgtgaaccag
    40381 cgtacttcct tggcatcatc tccggcgatt agcggttctt ctccaataaa ggctaagtag
    40441 ctgactgtga cgacccagcc tcgcggatct cgattaggcg tactgaatgt atgcagttgc
    40501 tcaatatttt cttttgaaat aaccacacca gtttcttcct ttgtttctct taagacgctt
    40561 tctccagttg actcatcctt ttggacaaag ccaccaggca atgcccatga atttcgataa
    40621 ggatgccctt ttctttggat caacaaaact ttcagctgat cttcttcttt gttatagcac
    40681 atcagtacga tatcgacagt caaagagggc ttgtcatatt ctggacgttc ctgtttttga
    40741 taccagttaa gaaattcttc ttcacttgct ttatgttcat aatataattt ttcttcttct
    40801 ttagaagcaa aacggttcat ctgaccacct ccattgacag taaaagtact ttttacctta
    40861 ttgtagcaga ctctgaaaaa aaaccaagga tttacttgaa aaatctaggt tatcatttaa
    40921 atatgaaaaa taaatggaag tatgagattg aataaaaaga aaagcagata aaacggcaat
    40981 actttatacc gctcttcacg aatggcaaag aagccacaaa taacgatata tacgtcaatg
    41041 ataaaacgca aaatccaagc tgctgtcata aaactgttca aatgcccatg gaggttaaaa
    41101 taaccataca tacgaatata aaaatagctg aatattaaat gtgctaatga aagaaaagca
    41161 atactaagtg taaaaaaaag atgtttcttc tgcatgattg ttccagtccc ttactaagat
    41221 agatagtctt tattatacca cgtgtgaggg gagaaataaa aagccgtttt ttagtagata
    41281 tgaaacaaaa caaaaacccg catttattca acctattcat ccttttaaag gatagattga
    41341 acgtagatag aagtcgttta tccagtcttt ttcattttga aaaaaagcta acagcatgct
    41401 ttttttatgg tatactagca aggaaacgaa ttcgcatcat gcgataaagg aggaagagcc
    41461 ccgtggcaga aaaggaaact ttttatatca ctacgccaat ctattatcca agcggcaaac
    41521 ttcatatcgg caactcgtat actacgattg catgtgacgc aattgctcgt tataaacggt
    41581 tgatgggctt tgatgtgttt tatctcactg gagtggatga acatggtcaa aaaatcgaaa
    41641 caaaagcaga agaactaggt gtaaaaccgc aagaatatgt agataaaatg gcggctgatg
    41701 tcaagaaatt atggaaaacg cttgatatca gttatgataa attcattcgc acgacagatg
    41761 attaccataa agcagctgtt caaaaaatat ttgatcgttt attagaacag ggagatattt
    41821 acttaggtga atatgaaggc tggtactctg tttctgacga agaattcttt acagaaacac
    41881 aacttgcaga agtgtacaaa gatgacgaag gtaacgtgat cggtggtaaa gcgccaagtg
    41941 gacacgaagt agaattggta aaagaagaat catacttctt ccgcatgagc aaatacgcag
    42001 accgtctatt ggaatactat gaagaacatc ccgaattcat ccagccagaa tctcgtaaaa
    42061 acgaaatgat caacaacttt atcaaaccag gattagagga tcttgcagtt tcacgaacaa
    42121 cttttacttg gggagtacca gtcaagagtg atccgaaaca tgtggtgtat gtatggatcg
    42181 atgcattatc caattatatt actgcactag gatatggctc cgaagatgaa agtctattcg
    42241 aaaaatactg gcctgcagat gttcagatgg tcggtaaaga aatcgttcgc tttcatacga
    42301 tctattggcc aatcatgctg atggcccttg atttgcctct acctaaaaaa gtatttgggc
    42361 atggctggct attgatgaaa gacggaaaaa tgtctaaatc aaaaggaaat gtcgtttatc
    42421 ctgaaatgct tgtggaacgt tatggtttag atgcactccg ttattatttg cttcgtgctg
    42481 ttccgtttgg gtcagatggt gtctttacgc cagaagattt tgtttcccgt gtcaattatg
    42541 atctggcaaa tgatctaggg aacttattga atcgaacgat tgctatgatc aacaaatatt
    42601 gtgatggact cgttcctgac tatgcctcac ttgtcactcc atttgatagt gaactgtcta
    42661 cgacggctgc taacgttgtc ggacgttacc atgatgcaat ggaaaaaatg gaattcaata
    42721 cagcattggc tgaaatctgg gtgctgattt ctcgtgcaaa caaatacatt gatgaaacag
    42781 aaccatgggt attagcaaaa gacgaagaaa agaaagctga attgaatagt gtcatgatcc
    42841 atttagctga atctttgcgt atcgcagcga tattacttca gccaatcatg acagaaacgc
    42901 cagagaagat cttcaatcaa ctaggattgg actctgaaac aatgaatctt gaaggattac
    42961 actttggcga atttccttca ggaacaaaag tagttgcaaa aggtacacca atcttcccac
    43021 gtttagatat ggaagaagaa gttgctttta ttcaagaaaa aatgtctgaa ggtactcaaa
    43081 caaacgaaga tacagtcaaa tgggatccag aagaaacaga acttgtttca acgaaagaaa
    43141 aacaaatcaa atttgatgta tttgaaaaag tagaactaaa agtagcagaa gtcatcaact
    43201 gtcaaaaagt agaaggggca gataagttgc tgcaattccg actagatgct ggagacagcc
    43261 aagaccgtca aatcttgtca ggaatcgctg aattttatcc tgatccaagc gaattgatcg
    43321 ggaaaaaagt cgtcattgta gcaaatctga aaccacgaaa gatgcgtgga caaatcagtc
    43381 aaggaatgat cctttctgca gaagcaccag atggctcttt acaagtaatc gaagcaccaa
    43441 aatccatgcc aaatggctca gaaatcgcat agaaattcaa agggggtatg acactagttg
    43501 tgtcataccc cctttttccg aaatttgaga agcaattttc gtaaattcct tcttattact
    43561 tgaagccaat catataatac agaaacagag aactatccaa aataaatcct agaaaaagaa
    43621 gtacgagctt cgctgatttt tgttaattta gaataatact aataggaatc gtaaaaacag
    43681 aaaaaaagca taatgataaa gggtcgggca gattttaatt gattcatctg gtactctttt
    43741 ttattgtcct tacattcata aagtttgaat gcaatgactt tattacatct atatttatat
    43801 ctaacagtag cgttgatgtg ttaattataa gttctatcac tttatgttca attagtttgt
    43861 gctcttatat gtgtgattga aaatatagat aaaaaaacaa tcatcggctt tgacttcgat
    43921 gattgttttc tttattttgt aaaaatccta tcacttgatt ttatggtaac cttttatttt
    43981 tgtcttcaaa cggcttggta agagagcagt aagcagtgca aatgggatcg aaaagacata
    44041 ccagaaaatc gtacgtccga taccataatg ttgatagagc tttgtcaaca tcacgtgtaa
    44101 caagatcaac cctaaaagca aggagccagc tataaaccaa tgattatccc aatgcatgac
    44161 ttcactaagt gccatcactc caaaaggata gaaataaaaa tagatcccga ctaaggtcat
    44221 ccgccagttc tttgtaaatg ttccgatatt caataaaaag aaaatcgctg aaagcaaaat
    44281 accgataggc ggaaaaagcc caaccaaaaa ggaatacaac atcccccaca agatcatcac
    44341 aggacctttg atcaatgcaa cgattgcgat gaacaaagtc agcaatatta gtttttgtga
    44401 taacgttgca acagtcatta atccaaataa aattaatgtg aaccaatgcc aatgggactg
    44461 ctgttgttct ttacgggaaa aatcgacccg gttggataaa taatctgcta atttcggata
    44521 agaacgctta aaatccataa actgttcctc cttcgtggaa aaggtaggtt tagtatacga
    44581 aaaataaaaa agaaatacat ccgtcttaag agggagtttt tcactgaaag tggagaaaaa
    44641 agaatggtaa attaaaatat tctcagttat actgaaaatt ataccaaatt tcaaagaaga
    44701 aagaggatgg gaatgaaagg caaactaaaa cgagaaatca atctgtttgg cgcattagct
    44761 accgttatgg gaaccgtgat cggtgcaggc gtcttcttca aaactgctgc tgttactgct
    44821 agtactcagt ctacaagttt gacattgcta gcttggcttt tgggtggttt cttaacgatc
    44881 tgtgctggat tgacagtggc tgaattagct acagcaatcc cggaaactgg tggagctgta
    44941 aaatatattg aagctgcgta tggaaaattg cccagctttt tattaggatg ggcgcaaagc
    45001 ttgatttact ttcctgctaa tattgctgca ttatcgatta tctttgcgac acaattgacg
    45061 aacttattac aattatctac cgattatctt ttgttgattg cagtgatcac cgctgtttct
    45121 gtgacagggt tgaatttgtt agggacaaaa gtcggtgctt ctgtacaatc agtgacgttg
    45181 attgttaaat taataccaat tgctgtgatt gtcatatggg gattattgac cccaggacaa
    45241 ggaactgtcc agttatttcc aatcgaagct ggaaaagatg ttacttttgt cgaaggattg
    45301 agcagtgcgt tgttagcaac gttgtttgct tatgacggct ggttaggtgt cggtgcaatg
    45361 gcaggggaaa tgaaacggcc ggaaaaagat ttgcctaaag caattattct tggtttgagt
    45421 tttgtaacag tcgtttatct attgatcaat tttgtttttt tgaaaacttt gccgattgat
    45481 catcttgccg gtaatttgaa tgcagcttct gaagcatcag acgtcatttt tggaggaatc
    45541 ggtggaaagc tggtcactat tgggatcttg atctcggtat acggtgcatt gaacggttat
    45601 acactaactg ggatccgcgt gccttatgcg atggctttag aagacgagct acctttcagt
    45661 aaacagctga caaagctttc aaagaagttt acggttcctt atgttcctgc ggttttccag
    45721 ttagcagtag cttgtattat gatgagcctt ggttctttcg actttttaac agacatgttg
    45781 atttttgtga tgtggctatt cagtttactg atttttatcg gtgtctttat tttaagaaaa
    45841 aaagcgccgg aattgccacg tccatataaa gtaccacttt atccaatcgt tccgattatt
    45901 gcgattctcg gtgctatttt cattttaggt atgacgatga tgacgcaaac gaaattagca
    45961 ttgatcggta taggtgttac cttgataggt atcccagtct attatcaaaa aaagaaaaaa
    46021 cgctcagaag agtaaaatat ttcaacaaca gacagaaaaa gtacgaacta ggtcctctag
    46081 atcttcgtgc tttttctatt tcaataaaac aagttcttct attttgattt ttgctacttc
    46141 tgcaagtagt gcttatgtgc taaagtaaga atgaaaagga gagataaaca tgatatttga
    46201 ttctcatacc catttaaatg ctgaacagtt caatgaagat attccagaaa cgattcagcg
    46261 agcacaagaa atgggggtaa cgaaaatggc ggtcgtgggc ttcgatacgc caacgattga
    46321 aaagtctttg caattaagcc atgactatcc aaatatatac agtattatcg gctggcatcc
    46381 gacagaggct ggcagttata cgaaagaaat cgaaaataaa ttacaagaac aattgacttt
    46441 accaaaggtt gtagcattag gggaaattgg cttggattat cattggatgg aagacccgaa
    46501 agaagtccaa gataaagtat ttcgcagaca gattgctatc gcaaaagaaa tgaacctacc
    46561 aatcagtatt catactagag aagcacttga agacacgtat caaatcctaa aagaagaaga
    46621 tgttcgagat atcggtggaa tcatgcatag tttcagtggt gattatgaat gggcaaaacg
    46681 atttcttgac ttagggatgc atatttcatt tagcggtgtc gtcactttca aaaaagcatt
    46741 agacgtccaa gaggctgcag caaatgtacc aatcgaacga ttacttgtgg aaacagatgc
    46801 cccttatctg gcaccagttc cttttagagg aaagcgaaat gaaccaggct atactcgtta
    46861 tacagtagaa aagattgctg aacttcggaa tcttccgaca gaagaagtgg ctgcccaaac
    46921 atggacaaat gcgcaccgat tatttggtct aagcgaacat ggataagatg aagatccaag
    46981 agattatagt cgttgaagga aaagacgata caagaaggct gcgggaagta gttgatgcag
    47041 atacgataga aacaataggg tctgcaatca atgaagagat cctgatgcaa attgaacatg
    47101 cccaagagac tcgtggagtg atcgttttta ccgatcctga tttttcagga gaaaaaataa
    47161 gaaaaatcat tatggaagct gttcctcagg caaaacatgc ttttttgccg cgcacacaag
    47221 ctaaaccaaa gaaaaaggga agccttggtg tcgagcatgc aagtgatgct gctattttag
    47281 aagcattgaa aaaggtcgtg acgccagaca tttcccaaac ggctgtttct gaaatcacta
    47341 gacaagacct tttagactat ggtttaatag ctggggcaca agcaaaagaa aaacgagaaa
    47401 aactaggtga cgagttgcgc atcggttaca cgaacggcaa acaattggaa aaacgtttga
    47461 agatgtttcg tattacgccg aaagaactcg cccaagcaat gaaaatagtg gaggaacaac
    47521 atgagtgaat atcgtgaaat tgcgacaccc tctagaacaa aagagatttt attgaaacat
    47581 ggtttttcat ttaaaaaaag tttaggacaa aattttctga ctgaaccaaa tattttaagg
    47641 aaaatcgtag aaactgcgca aatagatgaa catacgaacg tgatcgaagt tggaccagga
    47701 atcggtgcat tgacggaaca attagccaag catgcaaaac aagtcgtagc atttgagatc
    47761 gatgatcgtt tgatccctgt attggctgat acgatgcaac cctatgacaa tgtgactatt
    47821 gtacatcaag atattttaaa gaccgatttg tcaacagctg ttagagaaac atttcatgag
    47881 gagcttccgt taaaagtcgt agcgaattta ccttactata ttacgacacc gatcatgatg
    47941 cattttcttg aatcagattt ggttgtggat gaactagtcg tcatgatgca aaaagaagta
    48001 gctgatcgta tctctgctga accaggaacg aaagcttatg gttcattatc gattgctgtt
    48061 caatattata tggaagccag tttagcattc atcgtaccaa aaacagtatt tgtccctcag
    48121 ccaaatgtag attcagcgat tttgaaactg acaagaagag atataccggc agtcgaagtg
    48181 acagacgaaa aagcattttt ccgattgaca aaagcggcat ttcagcaaag aagaaaaacg
    48241 ctttggaata acttgcagca ttcgtatggc aaagatgacc agacaaaagc atggttagca
    48301 aaaagcttgg aaacagctgg aatcgatcca aaacgtcgtg gggagacttt gtcattacaa
    48361 gaattcgccg cattaagcaa tgcgatgtca gaaaatatgc agtaaaaaaa gaggttgtga
    48421 cagaagttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac aaaaatagtt
    48481 cctcatgttt ttcgtttgtt tgggcttatt accgcagatg caagcttttg aacaccgttt
    48541 aagaaagagg ttgtgggcct gccgtttaac tctaagtcac aaggaacaat ttcaaaaaac
    48601 agcttctcat gtttttgagt tagttggtct tattacttga tgcaagcttt tgaccactat
    48661 tatgccgaac aaggagatgt gacacaaatt ttgtcacatc tcctattttt gtttcttgat
    48721 caaaaaattc ttatacttga tactcacact cattttgtat ggatttagaa aagaatatgt
    48781 gtcttgacag ttattagtaa gtgagttata ctaagtcgac atttatgtgt caagacatat
    48841 taggagagag aaaaaatgag aaaaaattca gttcgaaaac taacgatttc tgctttattg
    48901 attgggatgg gggtcattat cccaatggtc atgcctaaaa tcatgattcc accagcttcc
    48961 tttactttgg cgtctcatgt gccattattt attgcgatgt tttttacacc aggagttgca
    49021 gtggctgttg cgctaggtac gacatttggt ttctttttaa caacgccggt tatcatcgca
    49081 ttgcgagcat tgtcacatct tgtgtttgcg ttgattgggg cttggtactt gcaaaagcat
    49141 ccgagtatcg ttttaaaaaa tggacagttt acttttgcta attggcgttt ccaagggttc
    49201 aattttgtga tcggtgtgat tcattcttta gcagaaatgc tagtagtcag catcttttac
    49261 tttattggta atatgcctga aacatattat tctcaaggat atttttatac agtattcatc
    49321 ttgatgggga ttggtgggct tatccacagt ttagttgatt ataatattgc ttattttata
    49381 gcaggaacac tcagcaaata ttttgatatc cccgtattta cggcagcaaa aaaagaaaat
    49441 cggaaagagt cgattcaagg gtacgcgaag aaaacgacat attagacatg atctctcaaa
    49501 aaaaaaactc tcatctagta atggataata aatcctctaa gatgagagtt ttttgtccta
    49561 aaaagagctg caccaacatc ttctggcaca gctctaacag ggggcaattt atggaagttt
    49621 gatgacttgt tctgtttctt tatcaaagaa gtggcctttg ctgatgttga aggcaaggtc
    49681 gactatttcg cctggtttat ggaaatctct tgcatctact tttgagatga actcagtaga
    49741 accagttttt gtgtaaagca tcgtttcagc tccaagtaat tcggaaacga caacttctga
    49801 tttcactacg gcttcaggtg aagcttcaag ggctacttgt tcactgtgga tgtcttctgg
    49861 acgaataccg aagataactt cttttccttc ataacctttt tcaactaata ctttgttttt
    49921 tccttcagga atacgaagct taagcccatg tccatctgtg ataacaccat ttttcaatgt
    49981 cacattaaag aagttcatgg caggagatcc aataaagccg gcaacgaaca tattgacggg
    50041 tgtatcataa acttctttcg gtgtaccgat ttgttggata aatccgtctt tcatgataac
    50101 gattcggtca gccatagtca ttgcttctgt ctgatcgtga gtaacataga tggtcgttgt
    50161 ttcaagacgc tggtgaagtt tggcaatctc agcacgcatg gctacacgta atttagcatc
    50221 taaatttgat aatggctcat ccattaagaa gacttttgca tcccgaacga ttgcccgtcc
    50281 caatgccaca cgttggcgtt gtccaccaga taaagcagct ggttttcgtt tcaagtattc
    50341 tgtcagacct aagatctctg ctgcattgtc cacacgtttt ttgatgtctg ctttatcata
    50401 tttacgtaat ttcaagccaa aagccatatt atcaaaaaca gtcatatgag gatacaatgc
    50461 gtagttttgg aaaaccatgg caatatcacg gtctttaggt gccacgtcat tcatgacttt
    50521 tccgccgata tttagttctc cttcagagat atcttccagt cccgcgatca ttcgcaaagt
    50581 agttgacttt ccacaaccgg aaggaccaac aaaaacgata aattcacggt ctttgattga
    50641 tagattaaaa tcagttacag aataattttc tgcattgtca tattttttat atacgttttt
    50701 cagagccatt tctaccataa tacgttccac actttctttt ttagttttat taactattta
    50761 aagtataaat gaaagcgtta actaacttca atgtcatgtt gaacaagaat tttttctttt
    50821 ttttgtcaat gtgctagttt taagctttaa taaatatcca taagaaataa aaactgatac
    50881 actggagtaa ataagtatgt taacaatatc aaaaggagtg tctcttatga aaatcgcttt
    50941 gatcgctcat gaccgaaaaa aacctatgat ggtgaaatta gcaacagcgt ataaaacaat
    51001 attggaaaaa catgaacttt ttgctaccgg gacgactggg cagaggatca cggaagcaac
    51061 cggcttgccg atccatcggt ttaaatccgg accgttaggc ggggatcagc aaattggagc
    51121 catgatttcg gaggatcagt tagatttggt gatttttctt cgagacccgc tggctgcaca
    51181 gccccatgaa ccagatgtaa cagcacttat ccgtttatct gatgtctatg agattccgtt
    51241 agctacgaat atcggaacag ctgaggtgct actaagagga ctggaagccg gcttcctaga
    51301 ttttcgaaga atcgtccatg aaatagacaa gcatccactt tcattttgat ggataagcgt
    51361 atggaaaaat aacgtttaat gttcgcctat atgtagctat aaaagctaaa caaactgata
    51421 atatgcgata taatacgaac gtattggata atataaacaa taatattagt ttttcgtatt
    51481 tgcagtaaat gtgaaaatat gctatagttt ctgtgggaaa agatcccaaa aataatacag
    51541 agtagaaaat aaagcgttaa gtgctaagag gatgggatgt tgcctcttgg acgaaggacg
    51601 taatgttcgc ggttttattt tcgcattcgc tgcatgatac gatgtagcag ctcttaaagg
    51661 agatgctaaa aatgaagaaa caccggttag acgcacgtat gattgctatt atgggcttat
    51721 tgattgcatt gatggtcact ttgtcccgac tggtagcaat cgagacccca tttattaaaa
    51781 tcagtgtcac atttattcca caagtgatca tgggcattct ttttggtcct ttttggtcag
    51841 ggataggcgc agttttagct gatttagtag gaatggccct cttctcgaaa tctgcattct
    51901 ttattggatt tacattgaat gcatttattg aaggagcaat ctacggattc tttttctatc
    51961 gaaaagagat cacttggaaa aatgccattc tggctacgct tagtgtcacg ctgatcatca
    52021 acttattttt gacaccatta tggcttgcgt tgatgtacca tgtgccattg ttcagctggg
    52081 tcgtttgggc gccccgcctg ttaaaaacag taatatggtt accgattcaa tcaattgcca
    52141 tttattacgt aggacgtagt attccttaca aaaaaatttt aagaagttta gcgatccacg
    52201 ctaaat
//
LOCUS       DAFEPQ010000011        52140 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.011, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000011
VERSION     DAFEPQ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.011"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            330..1175
                     /locus_tag="LP543_000744"
     CDS             330..1175
                     /locus_tag="LP543_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL2320929.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="LP543_000745"
     CDS             1456..2676
                     /locus_tag="LP543_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBL2320930.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="LP543_000746"
     CDS             2673..5393
                     /locus_tag="LP543_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL2320931.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="LP543_000747"
     CDS             5377..6321
                     /locus_tag="LP543_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL2320932.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="LP543_000748"
     CDS             complement(6390..7400)
                     /locus_tag="LP543_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL2320933.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="LP543_000749"
     CDS             complement(7580..7924)
                     /locus_tag="LP543_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBL2320934.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="LP543_000750"
     CDS             complement(7917..8351)
                     /locus_tag="LP543_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBL2320935.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="LP543_000751"
     CDS             8489..9226
                     /locus_tag="LP543_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2320936.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="LP543_000752"
     CDS             9219..10430
                     /locus_tag="LP543_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2320937.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10634..11416
                     /locus_tag="LP543_000753"
     CDS             10634..11416
                     /locus_tag="LP543_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBL2320938.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="LP543_000754"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="LP543_000754"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBL2320939.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="LP543_000755"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="LP543_000755"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBL2320940.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="tadA"
                     /locus_tag="LP543_000756"
     CDS             14132..15061
                     /gene="tadA"
                     /locus_tag="LP543_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBL2320941.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="LP543_000757"
     CDS             15018..16067
                     /locus_tag="LP543_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBL2320942.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="LP543_000758"
     CDS             16064..16342
                     /locus_tag="LP543_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBL2320943.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="LP543_000759"
     CDS             16317..16787
                     /locus_tag="LP543_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBL2320944.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="LP543_000760"
     CDS             16804..17106
                     /locus_tag="LP543_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320945.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="LP543_000761"
     CDS             17123..17494
                     /locus_tag="LP543_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBL2320946.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="LP543_000762"
     CDS             17466..17825
                     /locus_tag="LP543_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320947.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="LP543_000763"
     CDS             17997..19004
                     /locus_tag="LP543_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL2320948.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="LP543_000764"
     CDS             19029..20213
                     /locus_tag="LP543_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBL2320949.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="LP543_000765"
     CDS             complement(20650..22233)
                     /locus_tag="LP543_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL2320950.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="LP543_000766"
     CDS             complement(22811..22954)
                     /locus_tag="LP543_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBL2320951.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23176..23655)
                     /locus_tag="LP543_000767"
     CDS             complement(23176..23655)
                     /locus_tag="LP543_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL2320952.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23728..24012)
                     /locus_tag="LP543_000768"
     CDS             complement(23728..24012)
                     /locus_tag="LP543_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2320953.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24090..24284)
                     /gene="ssrS"
                     /locus_tag="LP543_000769"
     ncRNA           complement(24090..24284)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="LP543_000769"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24356..25642)
                     /locus_tag="LP543_000770"
     CDS             complement(24356..25642)
                     /locus_tag="LP543_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBL2320954.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25780..26451
                     /locus_tag="LP543_000771"
     CDS             25780..26451
                     /locus_tag="LP543_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBL2320955.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26417..26905
                     /locus_tag="LP543_000772"
     CDS             26417..26905
                     /locus_tag="LP543_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBL2320956.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26918..27865
                     /gene="prmA"
                     /locus_tag="LP543_000773"
     CDS             26918..27865
                     /gene="prmA"
                     /locus_tag="LP543_000773"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBL2320957.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27866..28630
                     /locus_tag="LP543_000774"
     CDS             27866..28630
                     /locus_tag="LP543_000774"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBL2320958.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITKITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28863..31076
                     /locus_tag="LP543_000775"
     CDS             28863..31076
                     /locus_tag="LP543_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBL2320959.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31087..31533
                     /locus_tag="LP543_000776"
     CDS             31087..31533
                     /locus_tag="LP543_000776"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBL2320960.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31704..32963
                     /locus_tag="LP543_000777"
     CDS             31704..32963
                     /locus_tag="LP543_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBL2320961.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33052..33999)
                     /locus_tag="LP543_000778"
     CDS             complement(33052..33999)
                     /locus_tag="LP543_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL2320962.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34393..35685
                     /locus_tag="LP543_000779"
     CDS             34393..35685
                     /locus_tag="LP543_000779"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBL2320963.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35700..37469
                     /gene="aspS"
                     /locus_tag="LP543_000780"
     CDS             35700..37469
                     /gene="aspS"
                     /locus_tag="LP543_000780"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBL2320964.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37578..38453
                     /locus_tag="LP543_000781"
     CDS             37578..38453
                     /locus_tag="LP543_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL2320965.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38719..40602
                     /locus_tag="LP543_000782"
     CDS             38719..40602
                     /locus_tag="LP543_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBL2320966.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40677..40961)
                     /locus_tag="LP543_000783"
     CDS             complement(40677..40961)
                     /locus_tag="LP543_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBL2320967.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40951..41685)
                     /locus_tag="LP543_000784"
     CDS             complement(40951..41685)
                     /locus_tag="LP543_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBL2320968.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41814..42437
                     /locus_tag="LP543_000785"
     CDS             41814..42437
                     /locus_tag="LP543_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBL2320969.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42629..43750
                     /locus_tag="LP543_000786"
     CDS             42629..43750
                     /locus_tag="LP543_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBL2320970.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43757..46885
                     /locus_tag="LP543_000787"
     CDS             43757..46885
                     /locus_tag="LP543_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBL2320971.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46904..50098
                     /locus_tag="LP543_000788"
     CDS             46904..50098
                     /locus_tag="LP543_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBL2320972.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50244..50951)
                     /locus_tag="LP543_000789"
     CDS             complement(50244..50951)
                     /locus_tag="LP543_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL2320973.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51716..52114
                     /gene="spxA"
                     /locus_tag="LP543_000790"
     CDS             51716..52114
                     /gene="spxA"
                     /locus_tag="LP543_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBL2320974.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        17113 a         9068 c        10787 g        15172 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc ttgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatctcaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggctt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg aaactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccctggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttat
    23041 tttacttgaa ctcattgact gttttccctt tattttaaac attataaaga ccgatgagtg
    23101 cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg
    23161 caggtcgtct acgtgttaag ctttggtgtg gtctttagct ggcaattggt tttctaaatc
    23221 agttcggaca acaagcacat cgcaagctgc gtttctaata acatattcag aaacagaacc
    23281 aataaataaa cgttctacag cattcaatcc tgtggctcct aacataatca aatctacttg
    23341 attatcttct gggatttctc tggcaataat ttgttttggt gagccatatt ctacgacaga
    23401 ggttacatta ttcaagccag ctttcttcgc attgctttca taatctgcca atgtttgctt
    23461 tgccatttca gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga
    23521 acgtgtgtca atgacatgtg ctaatagaag ttctccgtta tttcggatag ctacattgac
    23581 cgccttttta aaagctaatt cagcttcatc agaaccatct acggcaacca tgattttttt
    23641 gtattgttgt aacataagca ttcactcctc aagtcaatcg tattgtactc ttttattgta
    23701 gaacacttta gggaaaaaga gaattattta ctgctttggt tcagcaattt tttttgcaaa
    23761 tcggatactg aaggtggcag aaacaataat tacgatcagt aaaaagacca aggcaataaa
    23821 gcgttgattg aaaaaagctg ttaaaatacc cattacgcct aaaacagcat atgcagcacc
    23881 gtaaaattgt aggaatcctt gatttttatc atttttgttg atcaatacaa aaaagatatc
    23941 ctgtttatta aataaataat agctaacgaa taataatact aaagtggcga taatcattaa
    24001 aatcctaatc atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa
    24061 gagcaaattg ctttattctt taaaagatgg taatctccgt taatgccgtt gtttgtcctg
    24121 tagtattgat cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa
    24181 atgataaggc atgttaccag cagtgacaca agacgggtcc caagatatca ataaaaatgt
    24241 tcggtcaaca cacaaagagg acaattcgaa catcacagat ttcccacctc tatagtagca
    24301 tatgaattta aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt cttgtctagt
    24361 tttttggagg atgctttttc cactctttta tacggtaata ctgttgaccc agagcctgtt
    24421 catatttccc tgtatctttt ggctgataat actgagcatt cttcagttta tctggtaaat
    24481 attgctgatt gacccaagcc tgatcaaaat gatgaggata ctgataaccc acgccacggt
    24541 tgagtgattt cgcacctttg taatggctat ctctcaaatg atcaggaaca tctccagctt
    24601 tgccctctcg aatatcagca agtgctgcat ctaatgccat gtaggcagaa ttggattttg
    24661 gcgacaagca aagatcgacg acgacgtctg ctagtgggat acgtgcctca ggcaatccca
    24721 atttttcagc agctaataca gcgttcactg tacgagccgc agcagcagga ttacctaaac
    24781 ctatatcttc ataaccgata accatcagac gccggcaaat acttgccaga tcacctgcct
    24841 caactaaacg ggcgagataa tgaagtgcag catccacatc acttccacga atcgattttt
    24901 gaaaagccga aataacgtca taatgagcat ctccattttt gtcatgggta agagcttttc
    24961 tttggacaca ttcttcaatg atcgacagtg taagatgaat ccttccttct ttatctgaag
    25021 gagtcgatag tgtagccagt tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg
    25081 cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc
    25141 gttctttatc ccttaaagca tgttcgactg ccaactggat gtcttgttct gttaatggtt
    25201 tgacttcaaa gatctgtgta cggctgcgga tcgctggatt gatggtaata taaggattct
    25261 cagtagtcgc tccgatcaag atgattcggc cactttctaa atgaggaagc agaaaatcct
    25321 gttttgtttt atctaatcgg tgtacttcat ccaataagag gatcactgta ccactcattt
    25381 ttgcttcttc cgctacaact tgtagatcct tttttgaatc tgtagcggcg tttagcatgc
    25441 gaaatgcata attagtagag ccggcgatcg cacttgcgat actcgttttt ccagtaccag
    25501 gaggtccata tagaatcatg gaagagagca tgcgagcatc gaccatgcgg cgtatgattt
    25561 ttccttcccc gactagatgc tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc
    25621 gataagccaa tggctgttgc ataggatttt cctccttctt gtttttacgt cccttaatta
    25681 tatcatattt agcaagatgc ctagaaagaa cagccttctg ttgttttgtt ttcatagctt
    25741 atccaactaa tattgtgtat gataaagtag gtgagaaaaa tgacggataa acaaaagata
    25801 gatgaatttt tttggaataa aacgacgcca gaagtggcac gggatttatt ggggatgtat
    25861 ttggagcatg acacaccaga aggaagatta gctggatata ttgtagacgc ggaagcatac
    25921 ctaggtccag aagacgaggc cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg
    25981 gcaatgtatg aaaaaccagg tacgatctac ctttacacga tgcatactca tcggattttg
    26041 aacatcatta ctcagccaga agggattcct caaggagtaa tgatacgtgc aatcgaaccc
    26101 gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc agatatcagc
    26161 aacggtccgg gaaaattagt ggaagcatta gcaattcctc aagaacttta tggtcagtct
    26221 atagcagata gttcgttacg cttagttttt gagaaaaaaa agacaccaaa aaagatcaat
    26281 gctttgccta gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt
    26341 gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa accaaatcga gaaagattgg
    26401 ggttggagga aagaaaatga aaaagaagga tctattaaca tatttagatg aaataattga
    26461 aaaacaagtg acagactatg atgtagcttt agattgggat acaaaaaatc atacgatcga
    26521 gatcgttgtt cgcttgtttg cagaaaataa agcacaattt gagattgatg atgcagaagg
    26581 gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc gtacttcttt ttaatcctca
    26641 gaaatctgtt tttgatgaac gcaattatct agcagtaatc ccttatgaag gaaaaaaagg
    26701 gttggcaaaa tcagtagctg attctttagt ggaatatcta aatgaagtat tagctcaagg
    26761 gcaaagcgat ttgcttgatt ttttggatga agacgatgac gaagctgtat tcgagcttca
    26821 ctgggataat caacaactag cagaattagt agaagtaaaa cagcaaaatg aagcagatac
    26881 ttatttaccg tacccaagtt attaaggagt gacagatatg aaatggaatg aagtaaaagt
    26941 agaaactgaa agtgaagcag tcgaagcagt tgctaatatt ttgatggagg caggggcgag
    27001 tggtgtcgcg attgaagatt ctttagatgt tgaacatttt cagtcggatc aatatggtga
    27061 gattttagat aaaaatgatt ttacatcgct gaaagaaggc gcactagtca tggcttattt
    27121 tccggaaacg actttcttac cggaaatctt gccttttatc aaagatcgta tcacacaatt
    27181 acctgagttt ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag
    27241 caactgggca acagcctgga agaaatacta tcatccggtg cgtattacgc gaatgctgac
    27301 ggttgcgcca agctgggaaa actaccagac agaagatcct ctagaaaaaa ttatcacact
    27361 agatcctggt atggcatttg gcacaggcac tcatcccacg acttccttga cattgacagc
    27421 actagaaaca gtcttgcgtg gtggtgaaac attgctagat gtgggaactg ggtcgggcgt
    27481 gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg atttagacga
    27541 agtagccgtt cgttcggcga aagaaaatat ggatatgaat gaaatagcca aagatgtcca
    27601 tgtatcagct aacgatcttt tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa
    27661 cattttagca gatatcatct tattgatgat cccagatgct tggcgtttac tgaagcaaac
    27721 aggcacgctg atcgtttcag gaatcattga agagaagaag caaatggttc tggatgccat
    27781 gttagaacaa gggtttatcg tggatcaaat tttccagcaa aaagactggt atgcaattat
    27841 tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt ttaaaagaat catatgaatc
    27901 aaaagactat tttgttttag aaggggaaaa ctatcaccac attgtacggg ttatgaggat
    27961 ggaacccgag gatgaagttt ttttggcatt caatgatcaa atagccattg ttgcagaaat
    28021 cacgaagatt actgaagagg ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa
    28081 ggaactgcca agttcgatta ccatagcgag tggctatcct aaaggggaaa agctggattg
    28141 gatcgttcag aaatccactg aattaggagc gcaagctttt cttggtttcc cggcatcagc
    28201 ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga caacaacggc ttgaaaagat
    28261 tgctcaagag gcagcggaac aatcccatcg gcagatcgtg ccatcagtag agttattgga
    28321 aacgaaacaa gatttatttg agcggctaga tgattatgac gtggtcttgg ttgcttatga
    28381 ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc
    28441 agatagtcga atcttagctg tctttggacc agaaggtgga ttatcccgcg atgaagtcga
    28501 ttattttgta gaacacaaag ggatactttg tggattagga ccaagaatcc ttcgtacaga
    28561 aacagcacct ctttatttat taagtgctgc tagctatcaa tgggaattaa tgaatccttc
    28621 tttcagctaa cgttcttcat taataaaaga gtatcgacag aaagaattga aaaaatagtc
    28681 aacaatttgt ataatagaaa caattattta tacaatggat acgaaaaatc gtttgaaaaa
    28741 gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac tgcacggatt ttgggacaat
    28801 tatcaaacag atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc
    28861 ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt agtcaataca
    28921 tgggtcccga acatgtagca tttgttgaga aagcatgcga atacgcgact gctgcacatg
    28981 acggacagtt tagaaaatca ggcgaaccct acattattca tcctatccaa gtcgcaggta
    29041 tattagcaga tttaaaaatg gatccccata cagtggctac aggcttctta catgatgttg
    29101 ttgaagatac agaaatcact ttagaagatc tgagagaaga atttggcgat gacgttgcta
    29161 tgttagtgga cggcgtaacc aaattaggga aaataaaata taaatcccac gaagagcagc
    29221 tagcagaaaa ccaccgaaag atgctgctag caatggctca agatttacga gttatcatgg
    29281 tcaaattagc tgatagacta cataacatgc gtacgttgaa gcacctgcga gaagataagc
    29341 aaagaaggat cgctcaggaa actttagaaa tctatgcgcc tcttgcacat cgtctaggga
    29401 tcagccggat caaatgggaa ttagaagata cagcacttcg ttatctaaat ccaaaacagt
    29461 attaccgtat cgtccacttg atgcagacga agagagaaga acgtgaaaaa tacgtaagtg
    29521 gtactgttga agatattcga atagcgacag aagagctggg gatttttgca gaaatctatg
    29581 gacggccaaa acacatttat tcaatctatc gtaaaatgaa agatcagaaa aagcagttca
    29641 acgaaattta tgacttgcta gctattcgag tgatcgtaga ttcaatcaag gattgttatg
    29701 ctgtattagg agcaatccac acaaaatgga aaccaatgcc cggcagattt aaggattata
    29761 ttgccatgcc aaaagctaat atgtaccaat ctttgcatac taccgtgatc ggtccggcag
    29821 gaaatccggt agaaattcaa atccgaacac aggaaatgca tgaaatcgct gaattcgggg
    29881 ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca gatggtatga
    29941 cgaaacaatt aagctggttc catgagatac tcgaacttca agacgaaagc tatgatgctt
    30001 ctgaatttat ggaaggcgta aaaggagata tctttagtga taaagtctac gtcttcacac
    30061 caaaaggaga cgttactgag ttaccaaaag gatccggacc attggacttt gcatacagta
    30121 tccatacaga tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc
    30181 ttgattacaa attgaaaaac ggagatatca ttgagatcat gacttctcca aattcatttg
    30241 gcccaagtcg cgactggttg aaattagttg ctactagcaa agcaagaaat aagatcaaac
    30301 gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca
    30361 aatgtattac agatctagga tttacgccta aagatatttt gacgaagaac aaactgcaag
    30421 aagcactcga tcgttttaat tatcaaacag aagatgatct ctatgcagct gtagggtatg
    30481 gagaagttag ccccttgacg atggccaatc gtctgactga aaaagaacgt aaagaacaaa
    30541 aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg
    30601 acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca aggggtagaa aacttactaa
    30661 ttcgtatcag tcgctgctgt aatccgattc ctggtgatga tatcgttggt tatatcacta
    30721 aaggcagagg gatatccatt catcgtcgag attgtccgaa tgttcagcct gacaaaccaa
    30781 atgtagcaga acgtttgatt gaagtcgaat gggaagatac atcgaataca cgaaaagagt
    30841 atgatgcaga tttggaaatt tacggctata atcgttcagg cttattgaat gatgtacttc
    30901 aaacagtcaa tgcgctaacg aaaaatctca acagcgttga agcacggacg aataaagata
    30961 aaatggcgac gatccatttg acggttggta tccagaattt atcccaccta aagagtatcg
    31021 tggataaaat caaagcagta cctgatgtct acagtgtacg ccggacgaat ggataggagg
    31081 acagatatga gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa
    31141 gaaattggaa aaattggcta cggcctactt gttttattag gcattcatga aaccgatacg
    31201 caaaaagatg tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa
    31261 caaggaaaaa tgaatattag tgtagaagat gtgggaggac aaattttaag tatctctcaa
    31321 ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga agccgcacga
    31381 ccagaaacag ctattccttt atacgaatca ttcaatgaag gaatcagaag tcgcggcatc
    31441 acagtcgcta ccggacaatt cggagcagat atgtcggttt ctctcgtcaa tgatggacct
    31501 gtaacgatca ttattgattc taaaaacaaa taaagtaatc tggttttcac tagtaaaaac
    31561 tatgaaacac gtcgcttaag ttagactgat aaactctaac ttaagtggct tttttgtctg
    31621 caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt atacaagtta
    31681 ttttttatag gaggttgctg aagatgaaag aagtaccagt agtagatatc aaaatcacag
    31741 atttaaaaaa attgtatcaa aaaggataca acatgttgga ggagttacgc catgaagcgg
    31801 acgctcccgt cgtcaaagca aagattttca ataaagaagc tatcacgatc tacggatcat
    31861 cagcagcaaa agtattttat gatccaagga atttcaaacg aaaaggtgct atgccaaaat
    31921 tagtattgaa gacacttttt ggtcaaggtg gagtacagac tttggatgga gctgctcatc
    31981 accatcggaa aaatattttt atggatctaa tgacaccgga aagaatggaa gattatcatc
    32041 gtatcttaga taagaatctg acacaagcac ttgaagcaca gcatggtcaa tttgaactat
    32101 ttgatttaag taagatggtt ttctttacat ctatttgcga gtgggcaggt atcaatttat
    32161 cagcgatttc taaagatgag gtcgaaaaat tagcagaata tcaaatctct atgatcagcg
    32221 gaacgtttac ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa aaatcagaaa
    32281 aatgggcgca aggcttgatt gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg
    32341 tggcattata tgcttttgcc aatgccacag atttagatgg tcagctttta cctctggaag
    32401 tggcagctgt tgaattattg aacatcatcc gtccaacggt tgctctaact gtgtgggcgg
    32461 cattgatggg acatgcactt ttcagtagac cagatttgta tcagcaatta aaaaatgact
    32521 tttcgacgtt acaagatccg tttattcaag aaatgagacg ctattatcct ttcttcccaa
    32581 tgctacctgc gatttcgctg aaagaagtag aagtagatgg ttatcgtata ccagaaggca
    32641 gttgggttat tttagattta tatggaacag accatgatga acgaacagtt gaagcgccag
    32701 attcctttat gatcaaacgg tatgtaggga aagcaaaaga tatttcctat aaagaagaat
    32761 atgagatgat tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat
    32821 ggatcactct tcatagtcta cgtgtgtttt ccgatcagct agtaaataag tttgagttct
    32881 ctgtacctga acaagattgg acgattccat tcaatcagtt tccgacttat ccgaatagcc
    32941 gagcgctact ttacaagaat tagattagca taaacttatc gaaaaaaagg aagatcaaac
    33001 agtgctgatc ttcctttttt catattacta taaattgata aggtcaattg atcaaaatcc
    33061 acgaaatgct tcatcaatgg tttgataatc ttcattggat aaatgcaaat ctaaagcttg
    33121 gacattgtca gatacttgct ctggacgctt agctccaggt ataactacag acactcgtgg
    33181 attcttcatg taccaagcta aaacaagttg cgcaggagtg gcttggtatc ttttagcaat
    33241 ggggcgaagt acatcgacag cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg
    33301 atttcttttt cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt catattttcc
    33361 tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt ttttttcaag
    33421 ataagggaat aattcttttt cagcctgccg atggatcaaa ctgtatttat cttctactac
    33481 atcgacatat ccatctgcat tcgcttcttt caattgttct aacgtgaagt ttgacacacc
    33541 gactgctcgg atttttcctg cttcttttaa ttcgtgcaat gtagcaactg attcattttt
    33601 tggtgtgctt tcatccggaa agtggatata aaaaatgtcg atgtaatcag tttgcagacg
    33661 ttttagtgca tcttccaccg attgtttgag aaactcagga gaattatcaa atgttcgccc
    33721 tttatttggt acatgagcag cttttgtagc gatgacgaca cgtgagcgat catattcttt
    33781 ttccttcagt acctcgccaa tcagttcttc ggaacggccg tttccgtaag cataagcggt
    33841 atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact acttgttttc ctgtctcatc
    33901 ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt gctcctaatc caagctgttc
    33961 tgcgtagact tgtgaatgtc cgattcttat ttctgccata gctgattcct cctttttgtc
    34021 tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca
    34081 gatatctctt gactttgaga ctggttttta gtaggttata agtaatatcg aatgtcaatg
    34141 aaagagcgag taagtttttg aaactttaca agagaggatc atcgaggctg aaaatgatct
    34201 aaagtttcat tagcctaaag acactctgga gatggacgag tgaaaagccg tcccgcgtac
    34261 gagcgttatt cgtaagagga aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat
    34321 ttcattcacc cttgttcatg atgaacaagg gtgatttttt tatttacata gctaagaaag
    34381 ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa atgatatttt gccaggcgaa
    34441 tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat tattcagaga ttatcagtat
    34501 aatgaaatgc gtacaccgat ttttgaacac tatgaagtga tatcccgaag tgtaggggat
    34561 acaacggata tcgtttcaaa agagatgtat gatttttatg acaaagggga acgccatgtg
    34621 acgcttcgac cagaaggtac agcaccaatc gttcgttctt ttgttgagca taaattgttc
    34681 ggcccagagt atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa
    34741 cgccctcaaa aaggacggtt acgtcaattc catcaaatcg gagtagaagc atttggatca
    34801 gaaaaccctg caactgatgt ggagagcatg gtgatggcac ttgatttctt caaacagttg
    34861 gggatcacgc aaatccggct agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc
    34921 tatcgtcaag cattgatcga ctatctttta ccttttgaat cagagctgag tgaagactct
    34981 aaacgccgcc ttcatgaaaa tccattacgc gtattggaca gtaaagacaa acgagatcaa
    35041 aaatttgtgg cagaagctcc atctatctta gattatctaa gcgagcctgc tcaaaaacat
    35101 tttgataccg ttgtttccat gctggatgca ttggctgttc cttatgaaat cgacagtaat
    35161 atggttcgcg gactagatta ttacacacat acgatttttg aaatcatgag tgacgcacca
    35221 aaaatggggg cacaagcaac gatttgcgca ggcggacgtt atgacaatct agtggaagaa
    35281 ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg attactcttg
    35341 actatggagg cagaggaagt agttatccct gcatttaatg agttggatgc ttacgtggtc
    35401 gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt
    35461 ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt taagacagca
    35521 gataaagcga atgccaaatt agtattggtg atcggtgaag atgagcttgc tagtcaaacg
    35581 attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga agagatatat
    35641 gaacattttg atgaagtcta tgatgaaatg actttattcg agtaggagga aaaaaataaa
    35701 tggcacaaag aacgatctat tgcggattag tttccgaaca acaattagaa caaacagtta
    35761 cattaaaagg atgggtacaa aaaagacgag accttggggg agtaatcttt atcgacctac
    35821 gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa gaagcatggg
    35881 aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt gactggggaa gttagaagac
    35941 gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga attcgaagtg attgcaagtg
    36001 aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca aattgaagat gatcaagcaa
    36061 tcagtgacga aatacgtttg aaatatcgtt atttggatct gcgtcgtcca aaaatgacta
    36121 aaaacatcaa attaagaaat gcgacaacaa aagcgattcg tcattattta gatgataatg
    36181 actttctcga tatcgaaaca ccttacctag ggaaatctac accagaaggc gcaagagact
    36241 acctagttcc ttcacgtgtc catgctggac atttctatgc gttgccgcaa tctcctcaat
    36301 tgtttaaaca attgctgatg aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt
    36361 tccgcgatga agatttacgt ggagaccgtc agcctgaatt tacgcaagta gatattgaaa
    36421 caacgttctt aacagcagaa gaaatccaaa cttacacaga gggattgatt gcgaaggtaa
    36481 tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc tatgatgaag
    36541 caatggctcg ctacggcagc gacaaaccgg atacacgttt tgatatggaa ttgatcgatc
    36601 taagcgatac agtcaaagaa gtagaattca aagtcttcca aatggctctt gaaaacggcg
    36661 gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg ctactcacga aaagacatgg
    36721 atcagttagg tcaatatgta ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag
    36781 aagaagacgg attgaaaggt ccaattgcta aattcatggg agaagcaaca gaagcaatca
    36841 ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa
    36901 tcgtcgctgc tgcattaggt gctatccgga cacgtttagg aaaagaatta ggactaattg
    36961 atgaatcgaa attcaatttc ttatgggtaa ctgattggcc acaatttgaa ttttcagaag
    37021 aagaaggtcg atatgtctct gctcaccatc cattcacgat gccaaaagaa gaagatatcc
    37081 cgttattggc tgaagaccct gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg
    37141 gttatgaatt aggcggaggt tctatccgta ttcatactcg tgagcttcaa gaaaaagtac
    37201 tagaaactct aggctttaca aaagaacaaa tggaagaaca atttggtttc ttgttaacag
    37261 ctttagatta cggattccct ccacatggcg gtattgcatt aggattggac cgttttgcaa
    37321 tgttattagc cggagaagat aatattcgtg aagtcattgc ctttcctaaa aacggaaaag
    37381 cagcagatgt gatgagtgat gcaccaagta tggtttcgcc tttacaattg ttcgaattga
    37441 atattgatgt aacgaatata gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat
    37501 acgcttcttt tgacaacctt gatttaattg aaaaacactt tataatgaag ccataacata
    37561 taaagcgggg cttttccatg aaaaaaacat tcgaaaattg ggcttatcat gattacacga
    37621 ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg aatttctttt
    37681 atcagccagg acatatttat tcaagtggta ttactggtct agctcagatt ttgacgactt
    37741 tgtcaaaatc actgatagga ttcgaagtac cggtatcaac aactttgtat ttactgaata
    37801 tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt ttcactttta
    37861 tcacagtcac tttgacctcc ctttgtatcc atattttacc ggaaacagtc ctatctaccg
    37921 atccaatcat ctgtgcgttg tttggggggg ctgtgatggg actaggtatt gggctagcgt
    37981 tgagaaacgg cctatcatcg ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa
    38041 caggaaagtc gattggttct atttcgatct acttcaatgc attgatcgtt tttgttgccg
    38101 gaattctatt tggctggcaa tatatgtttt acagtacgtt gtctattttc gtcagtggaa
    38161 aagtaactga tgtcgtttat actaaacaaa agaagatgca ggtaatgatc gtaacgaaac
    38221 atccagaaaa agtagtaaat gaaatccaaa actgtatgcg tagagggatc acgatcatca
    38281 atgaagcaga aggtgcttat aagcatgata agcagacagt attgatgacg atcgttacac
    38341 gatacgaact gcctctactt agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa
    38401 gtattgcaga aaacgtccag atacttggtc gtttctatga ggatggcatt tagatcgagt
    38461 ttgacaaaaa ttcatgaaat actgctgtag acagacgttc gtttgtctgc agtttttttc
    38521 taccatagta gaatcagcaa agaaaaactg cttttttatt tgaaattttc ttaaagttga
    38581 aaaaaagcat gtttattttt ttaaatttat gggatgatag aaaaaaatag tagagaaagg
    38641 acacgattct acttgctatt ttcacttaat acccgtatat tacgaatgaa aaaagtttat
    38701 gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact
    38761 gggtttgatg ggctccgttc attagctgtg gtgggtgtta tcttctatca ccttctgcca
    38821 actagtatga aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg
    38881 atcacggatc tgctgcggca ggaatgggaa caaaatggaa aaatcaatat ttggcaattt
    38941 tatgtcagaa gaatgaagag actctatccg gcacttgttt ttatgcttgt gacagcttcg
    39001 gcttatatca ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc
    39061 agcttactgt atgtcaacaa ctggtggcaa atcaataacg gtttatctta tttcgatcga
    39121 tttgctaatg agtcaccgtt tacgcatatc tggtctttag cagtagaagg tcaaaactat
    39181 ttggtttggc cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag aaaatggatc
    39241 ttttatactg tgttaggtac atccttcctc tccgccatct tgatgatgat cttgtataca
    39301 ccaggaggcg atccaacccg tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg
    39361 ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa
    39421 caagcgaaaa atattctgaa tatcgctgga ggaatctcgc ttttggcact gattctttgc
    39481 ttcttctttt tagatgatca ttacaatttc atctattacg gaggaatgct cctagtgagt
    39541 ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg
    39601 ctgacaaatc cagtatttac ttggattggc aaacgaagct atggcattta tctgtatcag
    39661 tttccagtta tgatttttta tgaagcaaag gtgactgata ttgccgatca tgttttgttg
    39721 catactttag tggaaatcac attgattcta atcatcagtg aactttctta tcgatatgtg
    39781 gagcgtccgc tagcacgttt tgattatggt cgtacattcc aagtgatgaa aggatggttc
    39841 agcaaaccga tcatcagtaa gagcaaacca tgggttgttc caggcacatt acttgtagca
    39901 attgcactcg ttggtttcgt tacggcaccg aaaaacgctg taacagccga tcaaaaacaa
    39961 ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa
    40021 aacaccacta gttcggttga ccaagcgatc cttgataagt acgagttgtc caaggcacaa
    40081 gggaataagg cacaaaaatt agaactaacg gcatttggtg attcagttat gttggatgct
    40141 gcaacggatc tacaagaact ttatccaaaa gtagtggtag atggagatgt cggacgacaa
    40201 ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt gaaagacact
    40261 gttctgattg gactaggaac gaatggctca ttctcggaag ctcagtttga tagtgtgatg
    40321 gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg
    40381 caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa tatgacgctg
    40441 attgattggt acagctacag taatgaccat agtgactggt tttatgatga tcaagtccat
    40501 ccaaatcctg aagggatgac acattacatt cacttagtca gcgaagcctt gctaggagga
    40561 agttcaagta cgaacagcag tgagacaaac gtgacagaat gatagctaca aaaaacttca
    40621 ggcaaaaaca tttgtttttg cctgaagttt ttttatattt accgtgtttt taacattcat
    40681 atagattcag gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg
    40741 gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga aaatacttct
    40801 gcatggatca tcttaacagg tcttcttttt tgaagacgag tatatttagc accagtacca
    40861 ttgttatgtt cctctaatcg tctagaaaga tcggttgtat atccaccata aaatgtctga
    40921 tctttacaga ataatacgta aaaataatgt tcatttgcca tagagcatct cacgaatctc
    40981 tgcagtgtat tctccatcca tattgtaagt gatcaatggt ggagcgactt tgaatccgtc
    41041 taatttgcct tctttgattc cttcaactaa caaaatattt gcctcttttc caggttttgg
    41101 gtaaacaaat tggatccttt taggtgcaat tcgatagtct tccatgacct gaatgatttc
    41161 taagaaccga tcaggacgat ggaccatagt aaagcgtcca tttgttttta gtagcttact
    41221 tgaaatatcg atcacttctt ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg
    41281 tttattaggg ttcgttttat ttgttggcag ccctttgaaa taaggtggat tacagacgac
    41341 taagtcacaa gaattatgtc gaacgacaga taagctgtct ttcagatcga tggtgtggac
    41401 agtcatttgt tcatttaaat ggttaagcgc gatactacgt cttgccatgt ctgctagacg
    41461 aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac
    41521 tgcgccattt cctgcacaaa ggtcaacaat cttccctttt ttaggtacac gggaaaagtg
    41581 agcaagcagc acagcatcta aagaaaaaga aaacactttg gaactttgga taatcttgat
    41641 atcttctgcg tataattggt cgattcgttc gtcttcgtga agcatggatt ccctccaaaa
    41701 tgaaaaaaat agtcgtctgt attatactat ttttattaaa aagaaccaag gtggcttgtc
    41761 aaacctctat tttttatgca tactatgacc ataagaaggg aaaggaagag actatgtttt
    41821 atcgatttat gcgcggggta gttcgagtac tattagcctt agttaatgga aatgcccgct
    41881 atgagaataa agaagtttta ccgcaagatg agaattacat actagttgct ccccatcgta
    41941 cttggtggga cccattatat ctggcggtag ctgcagcacc caaggaattc agctttatgg
    42001 caaaagaaga actgttcaaa aatccagttc tgcgatttat attaactcga tccaatgctt
    42061 ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa aacacctgta aagattttga
    42121 aatcgacaaa tttgagtttg atcatgtttc ccagtgggac aagacatgct acagaactta
    42181 aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc
    42241 aaggtccgtt gacacttggg gatctgttca agcgtcagag agtgacagtg cgtttcggcg
    42301 aaccaatcga cttatcagat atcaagaaaa cggataaaga aggtatggcg gagatcgaac
    42361 gacgaacaca agcagctttt gacaaattgg acaaagaagt gaaccctgat ttcaaatatg
    42421 aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg gtcttttgaa tctactgtcc
    42481 tgttcagttc agactttttc aatgaaaacg agggagagac gtcaagtctg ccctcttttt
    42541 gcgttccaat atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt gttataataa
    42601 ttagaaggac attttttgag gtgattgaat gcgtttttta catacagccg attggcatat
    42661 cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag cgtcatgtac tatcttctat
    42721 actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag acttatatga
    42781 taggagtgtc ccagctgttg atgccgtaga attatacaat gaattgatgg tcgattggaa
    42841 tttaaaggaa aagatcccga tttttgctat atcaggaaat cacgatagca gtacgagact
    42901 ttcagcgggt acgccatggt tcagttattc gaactatttt ttgcgaacac agctagcaga
    42961 agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt actttgaacc
    43021 aattgcagcg agattatatt ttgaagatga caatatccga acgatcaagc aagcggtagc
    43081 caaggtgata gataaaatgc aggagctatt tgattcagaa aaaaaacaag tattgattag
    43141 ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg
    43201 gttggattcg atccctggtg aactacttac accgtttgat tatgtagcac taggacattt
    43261 gcataatcaa gctgctttga gacaggataa tgctcgctat agcggttccc cattgaaatt
    43321 ctctttatct gagatcaacc aaaaaaaagg cgtatggatc gttgacctga caacaggcct
    43381 ggagttgaat tttagggaga tcgaacccct gcatgatatt gaagaaataa cagcgagttt
    43441 caaagaattg ctggatcctg ccttttatca gtccgtcaat cgggagaatt acatctatat
    43501 ctatctcact gatagaacga tcatcccgaa tatgatgaac cagcttcggc aaatatatcc
    43561 tcgtattttg gggattgaac gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa
    43621 agtactagaa atgaaagatc ctcaaaaatt agcagaagca ttttttagtg aaatgacgga
    43681 agagcagcta actgcaaacc aagaaaaatg gctcggagaa acgttgcaag aaatcatgga
    43741 aatggaatga tcaagaatga aaccgaaaaa attacaactt aaaaattttg gtccttttat
    43801 caatgagacc gtagattttt cacgtttgac agaagcaccg ctttttttga tcagcgggaa
    43861 gacaggagct ggcaaaacga cgatttttga tgggatcacc ttcgcacttt ttggtgaaac
    43921 ttccggaaga cttcgctctg gaaaagagat gcgttctttg tttgctacac cagacgaaga
    43981 aacaagcgtg actttttctt ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc
    44041 agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac
    44101 gttgaccatt tttgatggga aaggaaaaga aataaaacaa atcacaaaac gtacagaagc
    44161 agatcagttg atcagagaac tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt
    44221 actaccccaa ggagaattcc gtaatttttt ggtttcatca agcagtgata aagagactgt
    44281 tttacgtcat ttattcggta ctcaattttt ccaacagttc aatgagcgat taaaagaaaa
    44341 agcaaaacaa cagcaacaaa aattggatca tttagagcaa gagctgcatc ttctgcaaaa
    44401 aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg tttgaagccg ttttagccca
    44461 atggacaaac catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag aagctctaac
    44521 ggaacttcag gaaaaacaaa agcaactaga aaattcctac tatcgttttg aaagcttgaa
    44581 aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa aaggaattag ctgctcaaaa
    44641 agacgaaata gaggaaaaga aacgatggat tgggtattat gaatttacgc aacgattagt
    44701 cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa atagaactgt cacaaaaaag
    44761 tagtgagcaa acaaagaaac tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa
    44821 tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa
    44881 caaacagatg atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca
    44941 attaacaatt tcattggata aagaagaatt gaatgccgag aagctgtatc aaaatctcgc
    45001 tatctatcag gaaagaactc gaattcttca agaaaattta ctggatcagg aaaaactgca
    45061 agaagatcgt cttgcttttg gacagttaaa atatctagat gaaaagcagc aagaaataga
    45121 aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata agagaagaac tcaaaaaagc
    45181 aactgaagaa ataacaaaaa atagtcagct attgaaagaa cagacagaac aagcagaaca
    45241 gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt cagctttttt tgcgtccagg
    45301 ggagccatgt cttgtttgtg gatcaacgga acaccccata caacaattaa aacacgaaca
    45361 gccaaataag gaagatatct taaaagtaga acaacaactg gcagaagctg agtctgctgt
    45421 agaaagaact cagcaaatca ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca
    45481 attatcagat ttagaacaaa aaatcatggc aacgaatata caattggctg aacaagaaga
    45541 acacttgcaa caggctttga aaaaggcatt tcctgagata acagaacaaa aaacaaaaac
    45601 aactcttgta gtaatcgaac aatctttaga tagacgaaaa agcaagatgg ccaatgcaag
    45661 aatagagctg gaagaaataa gacaacaagc agaaaagctc gaacaacaat tagctgtcta
    45721 caaacagcag cagacagaaa aaaataatca gctccagcaa ctgcaaggag aaatcaaaag
    45781 tttagaacaa caattgcaag atacggatag taactgttta gaagaggaga tacgacgact
    45841 tgaaaaacag ttagaagaag actcgaatca actagctgtt gagcagaaag caggagtgca
    45901 attgttgcaa gatcttcata cgcttcggac acagcttgat cttcaacatg aacaaataga
    45961 aaaaatagat gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt tagaaagcca
    46021 gtcgtatttt tcaacaattg aagaaatctg tgcatttgca caacagcaag cacaatataa
    46081 agaaaaccaa cgtacagtta cttcttatca tgaagagcaa ttagtgacag ctgaccggct
    46141 gcgtcaatta gaaaatatcc agctaactga gacattccca gatacagaag agatcagcca
    46201 acagttagaa gaaataagac gtatgatcga aataaagcaa gaaaagatat acggtttaca
    46261 agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt ctttcgattt atcacacaag
    46321 ccagacgaaa ttagatgagc ttacgcaatt acagcagctt tcgcaaacat tcaacggaga
    46381 aaatcctaag aagactagtt tggaacggta tgtgcttcag gtttatcttc aagaagtgtt
    46441 acaagtagca aatgatcatc tccaacgttt aacgaaaaat cgttatcaat ttgagctagc
    46501 ggataccatt ggcagttatc gagggaaaac cggattagaa atcaatatct atgatgatca
    46561 ggcaggtatg tctcgaagtg cgcataccct gtctggcggt gagagtttta ttgcagcact
    46621 ttctttagca ttagctcttg ccgatgtgat ccagacacaa gctggtggtg tagcaatcga
    46681 tgcattgttt attgatgaag gatttgggtc attagatgaa gaagctttgg aaatggcaat
    46741 ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag
    46801 agaattgaaa gaacgagtat tacaacaaat aagaattgat acagaaggta gcggacaaag
    46861 caaaatacgc tatcaacttg gataaaaagg gagattggta actatgaaat ggagtattcc
    46921 agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt
    46981 ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt
    47041 tgatttggat ggtacagcaa aagagataga ttactgccaa tgtccctatt gggaggaaca
    47101 taaatattgt aagcatacag ttgctgtgga actttattta cggtcgaaaa atctatctcg
    47161 tatcatgaaa aaagaccagc caatgctaga accggtaaaa gcaacaagtg agggagaaat
    47221 cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt
    47281 gattattgaa taccaagtag aaacgattga gactaatcag tatcacccag agctttctat
    47341 tttggcactt tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa
    47401 aaacatctat gattttttac aagcttacac agaaaaagaa acttacactg ttaataaaca
    47461 gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca
    47521 tcttgcagga agtgctcaga cgcatcaatt attaggtacg aacggcttac aagtcaaagg
    47581 aaaactagat aaaagatatc tgcttctccc agtagaacaa gcccgcttcc tgttagagca
    47641 gatgaatcag actacacgtt tgcatctgca gataaatgaa caaaacgatc aaggaataca
    47701 attttccgat caaaacaaac ctttatcatt caaagtggaa aaatttggag aagattattt
    47761 gctgcaagca ttgaatgatt ttgatttttt ctttatgcat tatcagtggg gagttatcca
    47821 agggacattt tattctctga cgaaatatca acaaacggtt tatcaaactt ggaaacaatt
    47881 gattcgccga ttggagaaac cagaagtagt gttcaagaaa aaagaattac cgtctttgtt
    47941 taaagagatt atccctctgc tgtcagagat cggcgacgta gctattgccc ctgcagtaga
    48001 agaggaagta gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa
    48061 aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc tattcgactg acccgaagca
    48121 tgaggtctcg acagaacagg cttttcaggt gttacgtgac acagtagaag agaaacgagt
    48181 acttgatcta ttcagaatgt atcgctacca gaaaaatgaa actggttatg aacgtccatt
    48241 gccaagcggg gaagaactat atgccttttt ccgcacagaa ctatctgtct tccgtcagtt
    48301 tggtgaagtc agacttggaa gaaaattgcg agagttgtat ttagatgcgc ataaacatca
    48361 accgaaaatc gaagtaaccg agaccggttc atggctcgat attcgattcg atgtgacagg
    48421 gatagaggag caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg atgcttttta
    48481 tacattagaa aatggtcagg ttctttcttt tgattcagaa gaatttcaac aaacaagcca
    48541 agtgttgcaa caattacggg aatcgatacg tacagaagaa ggaacgatcc atgtacctaa
    48601 aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc
    48661 gttccaaacg atggtacaag atctcattca tccagaaaga taccaggcac agcttcccaa
    48721 aggattgaat gcaacgatga gagattatca aaagcaaggt tttcgatggt tgaagatgtt
    48781 aggtcattat cagtttggcg gtattttagc cgatgaaatg ggacttggga aaacactaca
    48841 gacaattgcc tttttacttt cggaaaaaga agaacggaaa agcttctctg ctttgatcgt
    48901 agcgcctgcg agcttaattt acaactggca ggcagaggtg agaaaattcg ccccttcttt
    48961 aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg ctaaagacac
    49021 agatatccga gtgacttctt atgccagctt aagacaggat ctagctaatt atcagtcgca
    49081 aaaaattgat tatttgattc ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac
    49141 tgcacaagca ttgagagaat tagctgtacc tcagcggttt gcactgagtg ggacaccaat
    49201 cgagaataat ttagaagaat tgtggtcatt gtttgcaacg attatgccag gatttttccc
    49261 gactaaaaca aaattcaggg aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt
    49321 tattttacgg cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa
    49381 tctatattct gcacttacag aagaacaaaa aactgtttat ttagcttact tgcgtcaaat
    49441 gcgtgaagaa atcacatcaa tggattctga agcattcaag aaaaaccgga tcgggatact
    49501 tgctggtctg acaagactgc gacaaatttg ctgcgatcct cgtctgttta ttgaagatta
    49561 ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa
    49621 caaacgaaga gttttgctgt tttctcaatt tacagggatg ctgactatct tgcaagaaga
    49681 gttagccgag ctagggatct cgacatttta tttaagaggc agtacgaaac cacaagatcg
    49741 gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat gtttttctga tttccttaaa
    49801 ggctggggga actggattga atctcacggg agcagatacg gtcatcttgt atgacctttg
    49861 gtggaatcct gccattgaag aacaagctgc tggacgtgca catcggatcg gacaaaaaaa
    49921 tgtggtagaa gtgtggcgta tgatctctga aggaacgatt gaggaacgca tggattcact
    49981 ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag aacagttgac
    50041 gaaactgact gaagaagaca tccgcatgat tttaagtgta ggagaaatcg aggagtaaga
    50101 gactcgaaaa agcgttcagt aatgggtaag aaagcctttc taaagaagcg attgtttatt
    50161 tgatttaaaa aatagcaagg aggctgggat tccattttat cccagcttcc ttgctatcat
    50221 ttttttattt ctatttttaa aggttataca aagattgtct tcattgtctc taacagctga
    50281 tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg
    50341 ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta gaacatcatt gtcgtaatta
    50401 tcgccaacgt ataaggtctc ttctggaagc atatgaaatt ctttctcagc cagctgaaaa
    50461 atctcttttt ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga
    50521 atccaattgt tcaattgaag ttgcttcagt ttttttgtct gatggtcagt cggcccattt
    50581 gtgataattc ctaatgggac attcttttcc ttcaagaaat cgagggtttt tttgacctcc
    50641 tcatgcaagc agatattatc aagttcttcc tcgtaaattt tttgaaataa gaggccatct
    50701 tcttccgtaa tatgagggta gtctaaatct tttagtgatt ggctgatccg atgtgctcgc
    50761 atatattcta gtgtccaatc tcctgccatt acttttggga agttttcatc actatgatgg
    50821 cggaaccgga tatatagtgg atgcatatct gcatctgaaa caagcggaac gactcttttg
    50881 accgcgtttc gaaatggctg ctgctgatca tacatcgtat catcaacatc aaacacaact
    50941 gatttaatca aaattttaca cgcctttcgg ttttcattga ttttttcaca aatccttgac
    51001 tattgtaaca gaaaaaattg tctaaaaaag ctgataaata aaggtttttc ttactatatt
    51061 tctatttcct tgacagaata aggtttttat atttcagttt ttttcactaa aaatcgtgaa
    51121 acacgtcaat aagtttgatg aacaaattaa tcattatggt agaagtcgat tgatttattt
    51181 taatgcaatc tctgtggaat tttcgaaaga tttatttttt taatacagtc tcaatataac
    51241 tattcaaaca taaaataacg ttatttgatt tatttgttta tagtaaattt tatttagtaa
    51301 tcattgcttt aaaatactac gcttatataa cagagaaaat aagcgttata ataaagaaca
    51361 gtattattta aaatgacaga aaacattact attaaagaaa taaaaaacat aaaaaatctt
    51421 aagaaatcgg tttcatttag cttttgagat aaaataatag tacagtttta tacgctgttt
    51481 catatgttta gaaaatttga attagatttg aaaactctaa tttttaaatg ataaaaaaag
    51541 cgagaatgct ggttttactg tatattaacc ttagataact ttcatttttt taaaaaaatt
    51601 atgaaaaaaa ggtagctatt taaaagttga ttatgtataa tagaaacaga tgggagatta
    51661 ttatgatctt ctgttacaaa aaagtgattc ttatcacgaa aaggagtggt gcacgttgtt
    51721 aacattatat acttccccaa gttgtacttc ctgcagaaag gctcgtgcgt ggttgcaaga
    51781 acatcagatt ccatttgttg aacgtaatat tttttcagaa ccattgaaca gttctgaatt
    51841 aaaagcgatt ttacaaatga cagaagacgg aacagaagaa atcatctcga cacgttccaa
    51901 agtattccaa aaactgaaca tggatttaga tgatctgccg ttacaagaat tattggaact
    51961 tgtgcaaaac aatcctggat tgcttcgccg tccaatcatg attgatgata aacgtctgca
    52021 agttggtttt aatgaagatg aaattcgtcg gttcctaccg cgcgatgtac gacaattaga
    52081 attacgtcaa gcacaattga tggctggatt atagaaggat aatacgaaac ttggaaaaga
//
LOCUS       DAFEPQ010000012        51002 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.012, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000012
VERSION     DAFEPQ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.012"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            117..971
                     /locus_tag="LP543_000791"
     CDS             117..971
                     /locus_tag="LP543_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBL2320975.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            972..2309
                     /locus_tag="LP543_000792"
     CDS             972..2309
                     /locus_tag="LP543_000792"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBL2320976.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            2314..2538
                     /locus_tag="LP543_000793"
     CDS             2314..2538
                     /locus_tag="LP543_000793"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBL2320977.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            2540..3430
                     /locus_tag="LP543_000794"
     CDS             2540..3430
                     /locus_tag="LP543_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBL2320978.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            3439..4257
                     /locus_tag="LP543_000795"
     CDS             3439..4257
                     /locus_tag="LP543_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBL2320979.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            4364..4813
                     /locus_tag="LP543_000796"
     CDS             4364..4813
                     /locus_tag="LP543_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBL2320980.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            4831..6513
                     /gene="recN"
                     /locus_tag="LP543_000797"
     CDS             4831..6513
                     /gene="recN"
                     /locus_tag="LP543_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBL2320981.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLEEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(6568..6684)
                     /locus_tag="LP543_000798"
     CDS             complement(6568..6684)
                     /locus_tag="LP543_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBL2320982.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(6757..7713)
                     /locus_tag="LP543_000799"
     CDS             complement(6757..7713)
                     /locus_tag="LP543_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL2320983.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            7884..8252
                     /locus_tag="LP543_000800"
     CDS             7884..8252
                     /locus_tag="LP543_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBL2320984.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            8422..8853
                     /gene="mraZ"
                     /locus_tag="LP543_000801"
     CDS             8422..8853
                     /gene="mraZ"
                     /locus_tag="LP543_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBL2320985.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            8874..9833
                     /gene="rsmH"
                     /locus_tag="LP543_000802"
     CDS             8874..9833
                     /gene="rsmH"
                     /locus_tag="LP543_000802"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBL2320986.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIGLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            9838..10239
                     /gene="ftsL"
                     /locus_tag="LP543_000803"
     CDS             9838..10239
                     /gene="ftsL"
                     /locus_tag="LP543_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBL2320987.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            10240..12432
                     /locus_tag="LP543_000804"
     CDS             10240..12432
                     /locus_tag="LP543_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBL2320988.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            12458..13420
                     /locus_tag="LP543_000805"
     CDS             12458..13420
                     /locus_tag="LP543_000805"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBL2320989.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            13434..14795
                     /locus_tag="LP543_000806"
     CDS             13434..14795
                     /locus_tag="LP543_000806"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBL2320990.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            14821..15909
                     /gene="murG"
                     /locus_tag="LP543_000807"
     CDS             14821..15909
                     /gene="murG"
                     /locus_tag="LP543_000807"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBL2320991.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            15924..17144
                     /locus_tag="LP543_000808"
     CDS             15924..17144
                     /locus_tag="LP543_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HBL2320992.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            17300..18625
                     /gene="ftsA"
                     /locus_tag="LP543_000809"
     CDS             17300..18625
                     /gene="ftsA"
                     /locus_tag="LP543_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBL2320993.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            18648..19889
                     /gene="ftsZ"
                     /locus_tag="LP543_000810"
     CDS             18648..19889
                     /gene="ftsZ"
                     /locus_tag="LP543_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBL2320994.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            19906..20583
                     /locus_tag="LP543_000811"
     CDS             19906..20583
                     /locus_tag="LP543_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBL2320995.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            20597..21196
                     /locus_tag="LP543_000812"
     CDS             20597..21196
                     /locus_tag="LP543_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBL2320996.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            21211..21498
                     /locus_tag="LP543_000813"
     CDS             21211..21498
                     /locus_tag="LP543_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBL2320997.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            21547..22329
                     /locus_tag="LP543_000814"
     CDS             21547..22329
                     /locus_tag="LP543_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBL2320998.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            22350..23057
                     /locus_tag="LP543_000815"
     CDS             22350..23057
                     /locus_tag="LP543_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBL2320999.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            23319..26117
                     /gene="ileS"
                     /locus_tag="LP543_000816"
     CDS             23319..26117
                     /gene="ileS"
                     /locus_tag="LP543_000816"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBL2321000.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(26166..27692)
                     /locus_tag="LP543_000817"
     CDS             complement(26166..27692)
                     /locus_tag="LP543_000817"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBL2321001.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(27707..28354)
                     /locus_tag="LP543_000818"
     CDS             complement(27707..28354)
                     /locus_tag="LP543_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBL2321002.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(28538..28867)
                     /locus_tag="LP543_000819"
     CDS             complement(28538..28867)
                     /locus_tag="LP543_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBL2321003.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            29044..29772
                     /gene="sfsA"
                     /locus_tag="LP543_000820"
     CDS             29044..29772
                     /gene="sfsA"
                     /locus_tag="LP543_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBL2321004.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            29788..30801
                     /locus_tag="LP543_000821"
     CDS             29788..30801
                     /locus_tag="LP543_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL2321005.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            30801..32078
                     /locus_tag="LP543_000822"
     CDS             30801..32078
                     /locus_tag="LP543_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL2321006.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            32141..34843
                     /locus_tag="LP543_000823"
     CDS             32141..34843
                     /locus_tag="LP543_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL2321007.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            34995..35312
                     /locus_tag="LP543_000824"
     CDS             34995..35312
                     /locus_tag="LP543_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2321008.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            35342..35662
                     /locus_tag="LP543_000825"
     CDS             35342..35662
                     /locus_tag="LP543_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL2321009.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            35748..37209
                     /locus_tag="LP543_000826"
                     /pseudo
     CDS             35748..37209
                     /locus_tag="LP543_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            37277..37498
                     /locus_tag="LP543_000827"
     CDS             37277..37498
                     /locus_tag="LP543_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321010.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            37529..37711
                     /locus_tag="LP543_000828"
     CDS             37529..37711
                     /locus_tag="LP543_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBL2321011.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            37711..38124
                     /locus_tag="LP543_000829"
     CDS             37711..38124
                     /locus_tag="LP543_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBL2321012.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            38247..39428
                     /locus_tag="LP543_000830"
     CDS             38247..39428
                     /locus_tag="LP543_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2321013.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(39499..39663)
                     /locus_tag="LP543_000831"
     CDS             complement(39499..39663)
                     /locus_tag="LP543_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321014.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            39817..39890
                     /locus_tag="LP543_000832"
     tRNA            39817..39890
                     /locus_tag="LP543_000832"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39851..39853,aa:Arg,seq:cct)
     gene            complement(39959..41098)
                     /locus_tag="LP543_000833"
     CDS             complement(39959..41098)
                     /locus_tag="LP543_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL2321015.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(41397..42032)
                     /locus_tag="LP543_000834"
     CDS             complement(41397..42032)
                     /locus_tag="LP543_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321016.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(42145..42780)
                     /locus_tag="LP543_000835"
     CDS             complement(42145..42780)
                     /locus_tag="LP543_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HBL2321017.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(42814..43275)
                     /locus_tag="LP543_000836"
     CDS             complement(42814..43275)
                     /locus_tag="LP543_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBL2321018.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(43405..43836)
                     /locus_tag="LP543_000837"
     CDS             complement(43405..43836)
                     /locus_tag="LP543_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBL2321019.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDK
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(43854..44174)
                     /locus_tag="LP543_000838"
     CDS             complement(43854..44174)
                     /locus_tag="LP543_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2321020.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            44473..45249
                     /locus_tag="LP543_000839"
     CDS             44473..45249
                     /locus_tag="LP543_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBL2321021.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            45264..45467
                     /locus_tag="LP543_000840"
     CDS             45264..45467
                     /locus_tag="LP543_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2321022.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            45483..45821
                     /locus_tag="LP543_000841"
     CDS             45483..45821
                     /locus_tag="LP543_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBL2321023.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            45808..45987
                     /locus_tag="LP543_000842"
     CDS             45808..45987
                     /locus_tag="LP543_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321024.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(46030..46500)
                     /locus_tag="LP543_000843"
     CDS             complement(46030..46500)
                     /locus_tag="LP543_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321025.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            46587..47285
                     /locus_tag="LP543_000844"
     CDS             46587..47285
                     /locus_tag="LP543_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBL2321026.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            47463..47804
                     /locus_tag="LP543_000845"
     CDS             47463..47804
                     /locus_tag="LP543_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321027.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            47797..48468
                     /locus_tag="LP543_000846"
     CDS             47797..48468
                     /locus_tag="LP543_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBL2321028.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            48474..49160
                     /locus_tag="LP543_000847"
     CDS             48474..49160
                     /locus_tag="LP543_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321029.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            49163..49912
                     /locus_tag="LP543_000848"
     CDS             49163..49912
                     /locus_tag="LP543_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2321030.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            49924..50193
                     /locus_tag="LP543_000849"
     CDS             49924..50193
                     /locus_tag="LP543_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321031.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            50198..50362
                     /locus_tag="LP543_000850"
     CDS             50198..50362
                     /locus_tag="LP543_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321032.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            50355..50657
                     /locus_tag="LP543_000851"
     CDS             50355..50657
                     /locus_tag="LP543_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321033.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            50654..50815
                     /locus_tag="LP543_000852"
     CDS             50654..50815
                     /locus_tag="LP543_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBL2321034.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            50812..>51002
                     /locus_tag="LP543_000853"
     CDS             50812..>51002
                     /locus_tag="LP543_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321035.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGT"
BASE COUNT        17179 a         8575 c        10591 g        14657 t
ORIGIN      
        1 aattagaagc tggggcgtaa gtcctagctt ttttctatga atcatcataa acctgttata
       61 aatcgggaaa attctatggt agaatgaaaa cacaaaaaat atctgggagt ggttagatgg
      121 cagaattgat taacggaaaa gaattagctg aaaaaatgca ggcagagatt gctgaaaaaa
      181 tccaaaaatt gaaagataat ggtatccatc ccggattagt cgttttattg gtaggggaaa
      241 atccagccag ccaaatctat gtaagaaata aagaacgtgt agcaaaagaa atcggaatcc
      301 attcattggt agagcgttat cctgagacga tttctgaaga cgaactactt gcagaaattg
      361 aaaaatacaa tcaagatcct agttttcatg gtatattggt ccaattacca ttgccaaagc
      421 atatagatga agaaaagatc ctgttagcta tccgccctga aaaagacgta gacggttttc
      481 atccgatgaa tttaggcaga ctccttgcgg gtaaaccgga caagattcct tgtactccat
      541 acggcatcat gaaaatgttt gaagcttatt ctatcgatcc ggcgggtaaa cgagcagtag
      601 ttatcggacg aagcaatatc gtcggtaaac cgatggctca gctactcttg atggcagatg
      661 caactgttac tgttgctcat tctaaaaccg aaaatctggc tgaactggca caagaagcag
      721 atattttagt cgtagcaata ggacgtggac attttgtcac aaaagaattc gtaaaaccag
      781 gtgctgtagt gatagatgta gggatgaatc gtgatgctaa cggtaagctg atcggtgatg
      841 tgaaatttga tgaagtggag cctattgcag gctggatcac tcctgtgcca aagggtgtag
      901 ggccaatgac tatcacgatg ctgatgtatc aaaccgtaaa atctgccgaa gcttttggag
      961 caggtgaata gatggcacaa gaatatttaa ctgtcactac attaacgaag tatttgaaaa
     1021 gaaaatttga tgccgatcct tatttagaac gcgtatattt aacaggcgaa atatcgaatt
     1081 tccgtttacg tgccaatgcc catcaatatt tcagcttaaa agatgaccat gcaaaaatat
     1141 ctgctattat gttcaaagga gcattccaaa aattacgatt ccagccaaaa gaaggaatga
     1201 aagtccttgt cgttgggcgt atttcattat atgaagcggg aggttcttat caaatctatg
     1261 tagagcacat ggagcctgat ggaataggtg ctttatatca agaattggaa gaacgaaaag
     1321 aaaagctgat gaaggaaggc ctttttcaag ggcctaaaaa aatgctgcca cgtttcccaa
     1381 agcgaatcgc ggtccttacc agtccaagcg gagcggttat ccgcgatatc atcacgacgg
     1441 tgaaaagaag atatccaatt gtccaattgg ttttgttccc aacagttgta caaggaaacc
     1501 aagcagcgga caatgttgtc aggaatatcc agagagtaga agagatgggc aatttcgata
     1561 caatgatcat cggtcgtggg ggtggttcga ttgaggattt atggccgttt aatgaagagc
     1621 gggttgctcg ggcgatttat caagctaata cgccggtgat ttcatctgta ggacatgaaa
     1681 cagataccac gatcgctgat ttagtagcag atgttcgggc tgccactcca acagcagctg
     1741 cagaattagc tgttcctgtt ttgtcagaag agttgatgcg cattcaagaa cggcaagcta
     1801 gattgcagca ggcgtttgta cgccagatcc agcgcaaaca agaacggttc gagcgtgctc
     1861 gacgttccta tgtatttcgg cagcctgaaa gattgtatga aggtcagacc atcaaattag
     1921 accagttgaa acagcgatta tatcaagcta cacagcaaat ttatcatgcg aaagaaaaac
     1981 aaacgatcgc attaagccac caattggaac aagtggctcc gctttatcga gttaaagcag
     2041 ctcgccaaga aactgcttat ctagaaaaac gcttagaaga gaaaatccaa caatatatga
     2101 aacaacagca gcagcgtttt gctcaagctg ttcaatctct tgatttgttg agtccattga
     2161 aaatcatggg aagaggctac agctatacga cgttaaatga tcaagtagta aaaagtgtca
     2221 ccgagattca tcaaggggat ttgctgacga tccattatca agatggaatc gttaaaggac
     2281 aagtacaaga gatagacaaa ggagaataaa aatatggaaa atccaacttt tgaagagtca
     2341 ctgcaagaat tagaaaaaat tgtcatgcag ttagaacaag gagacgttcc attagaatca
     2401 gctctagatt cgtttaaacg aggaatggaa ttaagcaaac attgccaaga tacattagct
     2461 aaagcagaag agaccttgac gaaaatgatg acagaatcaa atgaagaagt tgattttgac
     2521 ggaaacgagg aaagctgaga tgagattaac tgacttttca gctattcatc tacctgctgt
     2581 agaaaaagaa atcttgtctt ttttagatga gcatacaacg gatagaggac tctatgatgc
     2641 gatgagttat tcagtgaaag caggcgggaa aagaatacgt cccttattac ttttaacggc
     2701 cgttgcttcc tttgatgagc ctattgatgt tcccgtctat caagtagcag ctgctttaga
     2761 aatggtgcat acgtattcct tgatccacga tgatttgcct gcgatggaca atgatgatct
     2821 tcgtagagga aaacctacca atcataaagt attcggcgaa gcattagcta tattggccgg
     2881 agatggtttg cttacaggag cattccagct aatcagtatg gcacacttag gtaattctcc
     2941 taagcttctg ctgcttcagc aattagctgt ttgtgcggga tcacaaggta tggttgctgg
     3001 gcaagctgca gatatcgagg gcgaatcgaa aaagctttct ttagaagaat tagcatttat
     3061 tcatgaaaga aagacagggc atttgattcg ttatgccttg ctggctggtg gcatattagc
     3121 gaagcaacct gaagaaatct tattgctact tcaacgatta gccgaacatt taggtttagc
     3181 ttttcagata agagatgatc tactcgatgt gattggtaca acgaaaacat tagggaaaac
     3241 agctggtaaa gatgaacgta tggaaaaaaa tacttacccg cgtttattag gcctagaaaa
     3301 gacacgtgag gcgttggaga ttgagctgtt atccgccaac aagatcatag acaaattaga
     3361 agaggacgtt ttttcttttg atggcgggct gatgcgccag atgatcaagc aatttgatgt
     3421 ggagaaatga gggaaagaat ggaaaaagaa cgcgtagatg tattagcggt caaacaagga
     3481 ttatttgaaa caagagaaca agccaaacgt tcagtgatgg ctggtttgat ttacaacgaa
     3541 aaaaacgagc ggtttgataa accgggagaa aaaattccag tgagtagtga attaagagtc
     3601 aaaggtaaaa aacttcctta tgtttctcga gggggattaa aattagaaaa agcactcaaa
     3661 gcatttgatc tatctgttga aggaaaaaca ttgttagata ttggtgcatc cactggcgga
     3721 tttactgatg cagctttgca aaatggcgca aaaatgagtt atgctttaga cgttggctac
     3781 aatcagctag cttggaaaat aagacaagat cctagagtcg tcgtaatgga aagagtgaat
     3841 tttcgttatg caaaacccga agactttacc ttaggaattc cagaagttgc tgttatcgat
     3901 gtttctttta tttcactaaa actgatgctg cctccattac atgctatttt aaaagaagag
     3961 ggagaagtga ttgctttaat caaaccccaa ttcgaagcag gcagagaagc tgtagggaaa
     4021 aacggaattg ttcgtgaccc tcagacccat caaaaagttt tagaagacat tttgtcattt
     4081 gcggcacaag gtggatatga tgtgctggaa ttaagttatt ctccgatcac ggggggagaa
     4141 ggaaatatcg agttcttggc tcacttgagg aaagtacccg aaagcggaac aatcaattca
     4201 gctattaata tggcagaagt ggtttcaaat gcacacgaac agtttgacca taaataaatt
     4261 gtaactgtga aaatcaagtg agaagtatgc ggattcttct ttattatacc aaggaaataa
     4321 gctatcattg attaagaaga atatgctaag gagcgaagac gtaatgagaa aaaaagatcg
     4381 gcatcgtttg attactcgat tgttgagtga gcaagatatc cgaaagcagg aagaatttgt
     4441 tgaaatcctg aaaagcaaag gaatatctgt tactcaagct acgatttcca gagatataaa
     4501 ggaactgaaa ctgatcaagg taccggcttc agatggcggc taccgttata gcataccagc
     4561 tgaaacaaac gaagatgtaa atactaaatt ggaaaaacta ttaaaagacg ctttcgtggc
     4621 agttgaccaa atggaaaaat ttgttatttt gaaaacattg ccgggaaatg cttcggcagc
     4681 agcaaatttg attgataaac gttacaagaa agaattattt tcgattatca atgatgacga
     4741 taatgttttg atgattacac gaactgaaga ccaggcagtt ttattgaaga aagacttcct
     4801 gcattacctt taagttgagg agcgaatgtg atgctacaag aaatcagtat tactaacttt
     4861 gcgattattc cggaattgcg gctttctttt cacgaaggaa tgaccgcgct gaccggagag
     4921 actggtgcgg gtaaatccat tattatcgat gctttaggtc tgttagcagg aggaagaggt
     4981 tcaagcgatt atatacgtca aggagcagaa aaatgtgtgc ttgaagggtt gtttgaacta
     5041 ccaaaacaag aaggattctc tgaactgatg gttgaattag ggatcgaaac agatgaggac
     5101 aatctgattg ttcgacggga tatgtcctta actggaaaaa atgtttgtcg agtgaatggt
     5161 catattatca cattagctaa tctgagaaga atcggcagtt atttagtaga tatccaagga
     5221 caaaacgaac atcaggaact gctccagcct gaatcacatc ttgctttatt ggatcgtttt
     5281 ggagatgtag ctttccaaca gaagaaaaag agttatcagc aagaatatat ctcttatcgt
     5341 gagctggaaa aaagggtacg aaaagtgcag caaaatgaaa aatcctatgt tcagagaata
     5401 gatatgctcc atttccagca agaagaaata gctgctgctg atttgcaagt cggagaagaa
     5461 gagaaattag aagaagaaag agaaaaactt agcaattatc aaaaaattgc tgatggatta
     5521 gctgcaggtt acggagcact gacgaataat gaacaaaaca gtgtagatgg tgttggcttg
     5581 gccgtttcag aactgcaagg gatcgcccat ctagatgtgg agtatgaagc aatttatgaa
     5641 aatatccaaa gtgcttacta tttgctgcaa gatgcaatcg gcgatatgag tcgtcagatt
     5701 gatttattgg agttagatga gagtcgttta gaagaagtta cgcaacgttt agaattaatc
     5761 cgacagttga aacgtaaata cggcgaatcc attgagtcaa tattggctta ctatgacgaa
     5821 atcaccgaag agctagcttc ttctgatttt tcagaaggtc aattggacaa aatgaaagag
     5881 gaactagagc aaaaagagct tctacttcag caacaagccg cagatcttca tgaagcgcgt
     5941 aaaaaaatcg caaaagaatt ggaaaaatcc attctgcatg aattgaaaag tctgtatatg
     6001 gaaaacactg agtttgaggt acgtttctta aaagaagaaa atagacaatt gaatcatgat
     6061 ggatttgacc aaatcgaatt ttacattacg accaatccag gagagccact gaagcctttg
     6121 gtgaaagtcg cttctggtgg cgaactgtct cggatgttgc ttgctttgaa aactattttt
     6181 tcttctgagc aaggtgtgac aagtattatt tttgatgaag tggatacagg tgtgagcgga
     6241 agagttgcac aggccatcgc agataaaatt tctcaaatat cgaaatactc tcaagttttg
     6301 tgcatcactc atctgcctca agttgctgca gtcgccgact atcaatatta tattgtgaaa
     6361 gcagtgattg atggacgtac gcaaacttct gtttcagagt tgaaaacaaa agaaagagaa
     6421 gaagaaattg cacgtatgct ggctggaagt gagattacaa aactcacact cgaacatgca
     6481 aaagaactat tgaaactcgc aaaaaggacc tgagccacct cgcagtggtt caggtccttt
     6541 tcagcagctg atagctatat tttattttta catgacactc attacagcag tcaatacaac
     6601 agatccaatc gttaaaatca gcattgtcca aatgacaaat tttgttactt tagcaaaagt
     6661 acttgtttgt tttttttcac gcattgtttt caacctcgtc attaattagt tgattcaagt
     6721 gtacaatctt ttttccattt ccacaagagg gcttttttat aaaaacttcc ctttttttaa
     6781 ataataggta gttacgatac aaataaataa tgttaataga actagccata taaaggcatc
     6841 atccctttca gcaataggta acttaatgtt cattccatag atactgccga cgattgtcgg
     6901 tatagttaaa ataatcgtta aagaagttaa aattttcata atattattca gattattcga
     6961 tacgattgct gaaaaagtct gattcatttg ttctactagt tttaattgaa tgcgagaagt
     7021 agtcattgcc tgctctgttt ccaccaatac atcacgtaaa tgatttttca aagcatgatt
     7081 ttcttggaaa agtttatttt gcgctagttc tttcaatgca tctaaattag cagaagtcgc
     7141 ttctctgaaa taaacgaggc ttttttggat atccatgatt tgataaagtt gtttgttttc
     7201 agtggatact tgaagtttgg cttccaaagt atcgcattga gattgaagcg tagctaaata
     7261 actgttgtaa gagagtgtga cctgccaaat gaattgaaag aagatattga gattaggcga
     7321 aatatcactt tttggaaagg tctggctaaa gactttcttt aaaaacagtg gttcatgatt
     7381 cgtaattgtg ataagcttct ttttgggcgt caaaataata gagagcgggt atgtatccac
     7441 ttgaaaataa ccgctaggac ttgttttagc aaaaggatat tgtaacaata ataacgctgg
     7501 ctttgtcaac tttgattgat tgaattcttc tgtacgggaa ttttctgcat cgtccaatat
     7561 ggaagtgatg taatctgacg gaatttcaaa ttgatgtgtc agctgatgaa tctcttcttg
     7621 attaggacgt tctacacaat accagatcgt ttggtcagtt tcctttccgg tattacgcag
     7681 tttctcatta tctaatgtaa aatatttgat catgattttc ctcctgttgc ctgtttcttt
     7741 tattataacg gatagggaat tggaatacat ggaatgtgtt tgtaagatat agaatataac
     7801 tgcataaatt taaataaaat gaaacagttg tgtaaagtat atacagtata cttgaagcaa
     7861 cgataaaaaa ggagtttacg ttcatgggca cattcacacc acttatcttg ttttggtata
     7921 tttacccggt aatcgttttg tttggttgcc aatttcttgt aaaattattt tcgttaaatc
     7981 gtcgttttaa aataaaagcc cctgatttgg cgattccttt tttgtggggc ggtatgcatg
     8041 cattgtctag aaatacaggg acaatttcta tcttaccgtt tttctttatt tctgttttgc
     8101 taatgggaat ttgtattgct gttttccaag catattatta tgaagaaatt atttatccgc
     8161 gttatttcaa gatgacttgg cgtttggttt ttctgttgac aatcgtcttg tacgctgtct
     8221 tgatcattgt gaacatactt tcttacctat gaaaaaaata gaaaagctgt aaaaagtctt
     8281 ttaaaaggct ttttgcagct tgatttttaa aaaaatgttt atgaataaaa aaagttgttc
     8341 ccggatttcc cagaaaaggt ggtagaaagt gggggattgt ggtagactaa ttttagttag
     8401 tggaggaatg gggggctttc aatgttgatg ggtgaattcc aacataatat tgatgcaaaa
     8461 ggacgtttaa tcgtgccctc aaaacttcgt gaagaacttg gcgaaaagtt tgttctgaca
     8521 cgcggattgg atggctgtct tttcggctat ccgatgagcg aatgggaaaa tctggaagct
     8581 aaactgaacg aaatgccgtt ggctaaaaaa gacgcacgta cttttgtgcg attcttctat
     8641 tcggctgcca ctgaatgcga acttgataaa caaggacgaa tcaatatacc cagcacgtta
     8701 agaaattatg cagctttgac aaaaggttgc gtagtcatcg gtgtatctaa ccgaattgaa
     8761 atttgggatg agacacgatg gcaagatttt tctgcagcag cagaagagaa ctttgacgaa
     8821 attgctgaaa gtatgattga tttcggacta taagaatagg aagaggctga ctaatgactg
     8881 aaacatttca acactataca gtcatgttga aagaaacggt agatggattg aacatcaaac
     8941 cggatggcgt ttatgtagac tgtacactcg gcggtgccgg acatagtgaa tatttattaa
     9001 gccaactaag ttctaaaggg catctgtatg cgtttgacca agatcaaaaa gcaatcgatc
     9061 acgccaagct gcgtttagct tcttatgtcg aacaaggtat ggtcacattt atcaaagcga
     9121 acttcagaga attagaagaa cgcttgagtg agcatgtaga taaggtagac ggcattttgt
     9181 atgatctagg ggtttcctct cctcagctag atgaagcaga acgtggattt agttatcatc
     9241 aagatgcacc gttggatatg cgcatggatc agtcagctcc tttatccgct tatcacgtaa
     9301 ttaatgaata ttcctaccat gaattagtaa agattttctt ccgctatggc gaggagaaat
     9361 tttccaaaca aattgctcgt gaaatcgaac gagtacgaaa aacaaaacca atccaaacaa
     9421 ctggtgagct tgtcgaaatc attaaacaag tcatcccagc tcccgcaaga agaaaaggcg
     9481 ggcatcctgc aaaaagaatt tttcaagctg tgcggattgc agtaaatgac gaactcggtg
     9541 ccgaagaggc ctctttagaa caggcaatcg ggttattgaa agtcaatgga agaatcagcg
     9601 taatcacatt ccattcattg gaagacagaa ttgtcaaaag tatgtttaaa gaatatagta
     9661 cggtacaaga tctaccaccg ggacttcctg tcatacctga cgaattccaa ccagaactga
     9721 aagtcattac tcgtaaacca atccttcctt cagaggaaga gctaacagaa aacaatcgat
     9781 cacgcagtgc aaagttacgt attgcagaaa aaataagaat gaaagaggag tagaatgatg
     9841 gcagaattaa agaaaatgca agattacgaa tatgatttac ctgaattggt ggatcagccg
     9901 gaacaacaaa tgcctgattc ttcttcgaat caagaaatca ttcttccaca atcaccggca
     9961 aaacgattaa agcagatttc tgctttggaa aaaattgctg tggcaagcgt ggcgattgct
    10021 attgttgctt tatgtgtttt aacagttatg ctgcgcacaa atatcagcgg agctgaaaaa
    10081 gaaatcacgt caatacaaac agaaatcaat gataaaaagc aagaaaaaac gagtttagaa
    10141 caagaaaaaa acgaactttc tcgtactgaa cggattaaac aaatcgcaga agaaaaagga
    10201 ttgtctatta atgacgacaa tttaaggaaa gtgaagtaaa tgagtcttaa aaataaaatt
    10261 cggcggttta tggaaaagaa aaatctaaat ccgatgaaca atcgcaaaaa agtaggtatc
    10321 attttatttg ctacgagtat tggattattc tttttatttg ccgtccgttt ctcgtatatc
    10381 gttattggag gtcatgttgc tggtacctca ttagctgaaa aaacaaaaca gttgtaccaa
    10441 ggaagtgaag tagtaaaagc aaaaagagga acgatttatg atcgtaacgg tgtggcgctc
    10501 gcagaagatg cctcctctta ttcaattaaa gcgatactct caaaaacata tacctctggg
    10561 gataaaaaac tgtatgttga agaaaagaat tttgataaaa tagcagaaat cctgcataag
    10621 aatctttcta tagacaaaaa agatgcactg aatattttag aggacggcgc aaaaaaagag
    10681 ctgtatcaag ttgaatttgg tagctatggg aaaaatatct cccaagaaac caagcaaaac
    10741 atcgaagcgg acatgaaaaa agaaggtgtc gctggtcttt attttgtcga ccatcaggct
    10801 cggatgtatc caaatggcgt cttttcttct cattttattg gatacgcagt tccagacaaa
    10861 gatgagaatg gcttagttgg taaactaggt ttggagtctg cttacaatga tatcttgagc
    10921 ggtaaagatg gtaagattat ttatcaaaaa gataattttc aaaatcctct gccaggtaca
    10981 gtggcagaag aagaaaaagc tgtcgacgga caagatatct acacgacttt agacagcaga
    11041 ctacaaagtt atttagaaac actgatggat caagtaaatg aagaatatca gccagaagaa
    11101 ttgacagcag tcctgatgaa agcaaaaacc ggagaaatct tggcaatggg acaacgacct
    11161 actttcaatc ctgaaacaat ggaaggattg actggaaaag atgctatctg gcgtaatttc
    11221 ttggtacaag atagttatga accaggatca accatgaaag ttttcactac tgcagctgct
    11281 atcgaagaag gagaattcaa cgagaatgaa accttccaat ctggaaaaat ccaagtagaa
    11341 gatgctacga tcaatgacca tgactttggt gaaaaaggtg tgctgaccat gcgtcaagct
    11401 ctttcatggt caagtaacgt cgggatggtc atactggaac agcgattagg cggacgatgg
    11461 tataactatt tacaaaaact gggatttgga caaagtactc attctggatt agatgatgaa
    11521 gtaaacggtg ctttacctac attaaatatt gtcgaccgag caatgagtgc ttatggacaa
    11581 gcggtaggtg tgacgaattt ccaaatgatg aaagctttca cttctattgc gaataacggt
    11641 acaatgattc aacctcgata tatcagcaaa gtggtggatc cccaaactgg agaagagcga
    11701 acaactcaaa cagaagttct agggcaacct ttttcaaaag aaacaacaga aaaagtccgt
    11761 gaatatatga gagatgttgt cgaaagcgaa aactacggta gtgcttatgg tgtatatagt
    11821 gtacctggat acaatgtatc ggcaaaaacc ggaacagccc aaatcgcttc tgatacaggg
    11881 ggatatcaaa caggagatac agcctacctg tattcgattg tggagatggt tccttctgaa
    11941 gatccagact atgtcttgta tctaacaatg aaacacccta agacatatga tcgaatggca
    12001 ttggctaaaa tcgcaaatcc attgatgaaa cgggcaatgg attttaaaga aactgaagaa
    12061 gattcagata cagaaacaaa aacagaaaaa gtatccgtag ccgattatcg taatcttgaa
    12121 gcggacgttg cagcagctga tgctcaaaaa agtggactgc aacctgtagt gatcggtaat
    12181 ggtaaaaaag tacaaaaaca atctactgcc aacggtgatc agttgatctc tggagaaaaa
    12241 ctgatcctat atactggtgg tgataagctg atgcctgacg taacaggatg gtcaaaagca
    12301 gatatcatga agctggggaa aatcctcggt attgaagtta gttttgatgg agatggttat
    12361 tgtgtcaaac aagaacttgc tccatatgaa aaaatcactg aagacaaact tagttttact
    12421 ttagaagaat aagaagagaa ataggagaga aaagagcatg gaatggacac aagcattgat
    12481 tcccattgtc agcagttgtg caatgacaat tgccgcaatg ccgctattta ttggttattt
    12541 tcaaatgaaa aagcaaggtc aggcgattcg tgaggaggga ccaaagtggc acaatagcaa
    12601 agccggaact cctacaatgg gcggcttagt gtttctgatc ggctctattt tgacaggaat
    12661 ttgggttgga gcttggcaaa aacagctaac tcctactctt ttcatcttgc tttttgtatt
    12721 agcgttatac ggagtaatcg gctttcttga tgattttata aaaatattca agaaaagaaa
    12781 catgggactg aactcgaagc agaaattgat tggacagatt ctagggggaa tcatctttta
    12841 tcttgtttac cgttctgaag gttatccagg tgtattgaat ttcttcggac tagatctacc
    12901 tttaggaatc atttatggta ttttcgctct cttctggctt gttggatttt caaatgcagt
    12961 aaatctgaca gatggcatcg atggattagt tgctggctta ggaagtatct cttttgcagc
    13021 ttacggtcta attgcttggc atcagcatca gtatgatgta ttaatcattt gcttaagtgt
    13081 tttaggaggt ttactgggtt tcttcgtcta caacagaaaa cctgccaaga tttttatggg
    13141 agatgtcggt tcattagcgt tgggcggact tttagcggca atttctataa tgctaaacca
    13201 agaatggaca ttgcttttga ttggtttgat ctatgttatg gaaacagcaa gtgtgatgct
    13261 tcaagttact tcctttaagc ttacaggaaa acgaattttt aaaatgtctc caattcatca
    13321 tcactttgaa atgtgcggat ggtcggagtg gaagattgat acagtttttt ggttgatcag
    13381 cattgtgact tcgttgatca cattatggat cgtttggtag gagatgacgg gaaatgaaaa
    13441 aaatatcaac atatgaaaat aagaaagtgc ttgtcttagg actcgcaaaa agcggagtaa
    13501 gtgcggccaa attattacat gaattgggcg cacttgtcac cgttaatgat ggaaaacctt
    13561 ttgacgaaaa tccggaagca caagagctgc tttctttagg gataaaagta ataacaggta
    13621 gtcatccaat cgaattgttg gatgaagaat tttcattgat ggtcaaaaat ccagggattc
    13681 cctacagtca tcctttagta gcaaaagccc aagaaatggg tattccggta attacggaag
    13741 ttgagctagc ttacgaagta gcggaatgcc caattattgg gattacagga acgaacggaa
    13801 aaacaacgac aacaacgatg acaggactac tactgaatgc aggagcagat caaggaattg
    13861 cacgcttagc gggtaatatt gggtatccag caagtggtgt agcgcaagaa gcaaaatcag
    13921 aagataaaat tgtgatggaa ttgtctagct tccaactcat gggaatcaca gatttccgcc
    13981 ctcatatagc ggtgattacc aatatctatg aagcacatat tgattatcat ggtacaagaa
    14041 aagaatatgt caaagctaaa tggaaccttc aaaaaaacat gacagaaaaa gattatctga
    14101 tccttaactg gaatcagtca gaactacaag aattagcgca aagaacaaaa gcacgcgtac
    14161 tgcctttctc gacaaaagaa gtgttggaag acggtgtgta tgctgacgat tactcaatat
    14221 actataaaaa agaaaaaata atggaaatat ctgaacttgg cgtaccaggt aagcataatg
    14281 tagaaaatgc actagctgct atttctgtag caaaattgta tggtatatca aatgaagcta
    14341 tacgagaaac gttgcatttt ttccatggcg taccacatcg tacgcaatat gtaggagaaa
    14401 tccaaggcag aaaattttat aatgattcaa aagcaaccaa tattttagcg actaaaatgg
    14461 ctctaagtgg atttgaaacg tctaaggtcg tgttattggc tggcggatta gacagaggaa
    14521 atacatttga tgaattaatt ccttcattaa aaggaatcaa agcaatggtc gtatttggcc
    14581 aaacaaaaga aaagttgatg gatgctggaa aaaaagcagg catcgagaca atagtaacag
    14641 ccgattctgt agaacaagca gttcctttag cattagaaaa cagtacagat ggtgatgtag
    14701 ttttattatc accagcgaat gccagctggg atcaataccc aaattttgaa actagaggaa
    14761 acagattcat ggaagctgtg aaccgattaa aatagaaaaa gtagaagcga gcgataaaaa
    14821 atgaaaattt tggttacagg cggaggaaca gggggacata tttatccagc tcttgccttt
    14881 gtcaattatg taaagactca agaaccgaat gctgaattca tgtatgtggg agcaaaacga
    14941 ggtctagaaa acaaaatcgt ccctgataca ggaatgcctt tccatacctt ggagattcaa
    15001 ggatttaaac gaaaaatttc catgcataac atcaagacgg tccgattgtt tcttaagagt
    15061 atccgtcaag caaaaaaaat cctcagtgat tttcaaccag atatcgttat cggtactgga
    15121 ggatatgttt ccggagcggt agtgtacgct gcagccaaaa tgggtattcc aacagttatc
    15181 catgaacaaa acagtgtacc aggtgttacc aataaatttt taagccgata tgtggatcgg
    15241 atcgctcttt catttaaaga tgcggcttcc ttttttcctg aaaacaaagc agttcttgta
    15301 ggtaatcctc gagcacaaga agtagcagat actaaaaaat cagatatttt gaaaacattt
    15361 gatttggatc cagaaaagaa aacagtcttg atttttggcg gtagtcaagg agcacttaaa
    15421 atcaatcaag cagtatcagt ttttttaagt gaatatattc cttcttctta ccaagttttg
    15481 tatgcttcag gagatagata ttatgaagag atcctaaata aaataaatcc tgaaatacaa
    15541 aatattagtg tccagcctta tatcaaaaat atggcagagg tcatggcttc cagtgatctg
    15601 ctggtggggc gtgctggtgc aacttcgatc gcagaattca cagctctggg gttaccggca
    15661 gtgttggttc ccagcccata tgttacgaat gatcatcaga ctaaaaatgc aatgagtctt
    15721 gttcacgcag gagcagcaaa aatgattgca gataatgaac taacaggtga atcactaagt
    15781 cagacagtca acgaaatcat gggtgatgaa gaattgcaaa agcaaatgtg ccgagcttct
    15841 aaagaacaag gaatccctga tgcttcaaaa cgtctttatg acttagtaaa acaaataatc
    15901 aaaaaataaa ggcggtggtc ttagtgagta agaaaaaaga accaaaaaaa ttgtctcatc
    15961 cgtcttctga tgaaaacaat ttgactcctt ggcaaaaggc taaccgaaaa tatctggcag
    16021 aacaagagat gaaaaaccaa gagccaaaaa aagaagacat aacattagat acaccccttt
    16081 cagattctga agaagaaaaa acggttcaag aggaacagca agaagaaagc cagtccgaga
    16141 aaaaaatcga attttttgaa gagatcaagc aaaagaaaaa cggtggacca tataacggtt
    16201 cttttttgaa ccggctgccc aatttaaaaa gtcaacggca caaagtacta tttcgtcggt
    16261 tagctttgat catcactgta ttgggtatac cgctgatttt tttgatctat tatgtttctc
    16321 cttatagcaa attacaagca gttgcagtat caggcaataa gacagtcaac agccaagaaa
    16381 tcatctctga tacaaaattg tcattaggag aaaatgtttg gggacagtat tttcatcgat
    16441 ctacttatat cgatcgcctt aaaaaagcac agccaagaat cgaaacagca aatatacatt
    16501 tcaaagggat gaatgaattc gatttggatg taacagaata taaagagatt gctttgatcg
    16561 caaaaaacaa tcaatactac ccagtgattg aaaatggaac agtattagat gaaaaagtag
    16621 caaatcctac aaaaaatctc cctatcttgg aagagttcaa agactctgca aaaatcaaag
    16681 aattaacaaa gcaatacaat cagttatcat cagaattaca aaaagcaatt tctgaaatca
    16741 agtacacgcc aagagcaagc aataaaaatc tgatccaatt gaatatgaat gacggcaatc
    16801 aagttattgt aaatatcaat aatcttgcga accaaatgaa atattattcg caagtagcca
    16861 aagatatgga tgaaaaaggt gtgatcgaca tggaagttgg tatcttctcg catccttacg
    16921 gtagcactca agaaacggaa gaatcgggcc aatcggaaga aacaactgaa agtgaagaaa
    16981 atgcggatca aacaaacgaa tcaagtgaag aggcagaaac gcaaaaagca caaacacctg
    17041 gatcgtctag cacaaacgga tcatctgatt caaatgattc aagtgaaaca agtcaaatag
    17101 aagataatcc aagtacaact ccgtcaagtt taaatgaaaa ttgataaaac aactttaaaa
    17161 agtaagaatg tactttctaa aaaccagtaa tttatggtag aattggaagc agtgaatata
    17221 tctattaata agtatatttt aaaacggacg ctagttgttc gcttgtatat ggaatagtaa
    17281 gtaggaggga atcccattca tggcaaaaac aggaatgtat gtaggccttg atattggaac
    17341 gacatctgtt aaagttgtcg tggctgaata tatcgacagc caaatgaata ttattggagt
    17401 aggaaacgca aaatcagaag ggattaaccg aggcattatc gttgatatcg ataaaacggt
    17461 ccaagcgata caacgagcag tacgacaagc agaagaaaaa gcaggtatcc agatcaaagg
    17521 cgtaagtgtc ggtttgccag caaacatgct tgaagtagaa aattgccagg gaatgattgc
    17581 agttaacgga gattcaaagg aaatcaccga tgaagatgtc agaaatgtgg cttcagctgc
    17641 ccttgttcgt tcaatacctc ctgaacgtca aatcgtgtca atattgccac aagattttac
    17701 tgtcgatggg ttcgagggaa tcaaagatcc tcgtggaatg atcggcgtaa gattagaaat
    17761 gtacggtctg ttgtttacag gacctaaaac gatcatccat aacatacgta aatgcgtgga
    17821 aaatgccgga ttaatcgtca acgaaatggt gattactcct ttagcgctga ctgaatcaat
    17881 tctttctgac ggagaaaaag attttggtac gatcgtcatt gatatgggtg gcggccaaac
    17941 aacaacggct gttatgcatg ataagcaatt gaagtttacc aatcttgacc aagaaggtgg
    18001 cgaatttgtc actaaagata tctctattgt attaaatacg tcatttaaca atgcagaagc
    18061 gttgaaaata aactacgggg atgcataccc agaacggaca tcaccagatg aagaatttcc
    18121 agtagatgtg attggtcagt cagaaccagt gaaagtcgat gaacgctact tgtcagaaat
    18181 tatttcagct cgaatggaac agattttcaa caaagcaaaa gatgcattgg accaaatcga
    18241 agcattagag ttgccaggtg gcgtcgtatt gacaggcgga gctgctagtc ttcctggtgt
    18301 tgtggacttg gcacaagaaa tcttcggcgt aaatgtaaaa ttgtatgttc caaatcacat
    18361 ggggctgaga aacccggtat ttacaaatgt aataagtatc gtggattatt cagcaaactt
    18421 gagtgaagtg tatcaattgg caaaaattgc tgttacgggt gaaacctcag cagctcgtca
    18481 aatggtagtt gaacaagaaa caacaaacac atatgagagc tatgaagcac ctgaagaaac
    18541 aatctacgat gagccggaac caaaagagtc cggagaaaat gtaaaaaata aaatcaaggg
    18601 tttctttaca aatatttttg attaattcat cctgggagga ataagatatg gaattctcaa
    18661 ttgataataa tatcaatgac ggcgcagtaa tcaaagtcat cggagtcggt ggtggcggtg
    18721 gaaatgctgt aaaccgaatg attgaagaaa acgttaaagg tgttgaattc attacagcca
    18781 acacagacgt gcaagcatta aaaaattcaa aagctgaaac agttatccaa ttaggcccca
    18841 aatatactcg tggtttaggt gctggttctc agccagaagt tggacaaaaa gcggcagaag
    18901 aaagtgaaca atccttacgc gaagctttag acggtgcaga tatgattttc atcactgcag
    18961 gaatgggtgg aggaaccggt acaggtgctg caccaatcgt tgcaggtatc gcgaaagaat
    19021 tgggcgcatt gactgttggt gtcgtgacac gtccattcac ttttgaagga ccgaaacgtg
    19081 gccgttttgc tgcagaaggt atcgctcgtt tgaaagaaaa tgtggataca ttattaatta
    19141 tctcaaataa ccgtttatta gaagttgtag ataaaaagac accgatgctt gaagctttcc
    19201 gtgaagcaga taatgtttta cgacaaggtg tacaaggtat ctctgatttg atcacagcgc
    19261 caggttacgt taaccttgac tttgctgacg tgaaaacagt gatggaaaac caaggtacag
    19321 cattgatggg gatcggtgtg gctagtggag aagatcgcgt agtagaagca acgaaaaaag
    19381 caatctcctc tccattgctt gaaacatcta ttgatggagc tgaacaagta cttcttaaca
    19441 tcacaggcgg tttggatatg acattgttcg aagcacaaga tgcttcagat atcgtagcaa
    19501 atgcagcaac tggcgatgta aatatcattc taggtacatc tatcaatgaa gaaatgggcg
    19561 atgaaatccg tgtgacggtc atcgcaacag gtatcgatga atcaaaaaag gaacgcaagt
    19621 ctagccgacc agctagacaa gcacaaatgc aatcacctgc tcaaaagact gtattagata
    19681 tggatcaagc taaaccaact tcttcagaag aagaaaacag ttttggcgat tgggatatca
    19741 gacgtgaaca aaatgttcgt cctagagttg atgattccaa ttttgacaat atcgaaaaaa
    19801 aagaatttga tacattcaac cgcgaagaaa caaaatctca tggcgatgat gaattaaata
    19861 cgccaccgtt tttccgtcgt aaaaaataaa ggagaggaag agcacatgat tgctgacaac
    19921 ttgcagaaaa tcaagcaaga aattcaggat tcgtgtgctc ttgttcatcg tgatccgaaa
    19981 gaagttacat tagtagctgt aacaaaatct gttgaatccg ctcaagctga agaattggtt
    20041 agagctggcg ttatgaattg tgcagaaaac cgagtagata aattattaga aaagaaacaa
    20101 gatttatttg attttcctga aataaagtgg catttaattg gtaatttaca gcgcagaaag
    20161 gtaaaattga ttgtaaatga aattgattac tttcatgcgt tggacagttt aagattagca
    20221 gaagaaatcc agaagcggtt agagaaagaa ttatcctgtt ttattgaagt aaatgtttct
    20281 ggtgaggaat caaaacatgg aatttcacca gatgagcttt taccgtttgt ggaatcttta
    20341 gctgcctgca caaaaatcaa ggttgttggg ttgatgacta tggcgcctaa agaagcttct
    20401 gatgagcaaa tccataaagt gtttggtacg ctgaaagaat tgcgtgatct tgttcaagca
    20461 aaaggttatg cccatgcacc atgtagtgaa ttaagtatgg ggatgagtca agactttcaa
    20521 attgcgattg aagaaggtgc aacatttatc cgtgtaggaa cagcgctatt caaagacgaa
    20581 taaaggagga acaaaaatgc cgcttttaaa cagaggagca attgctaatt ttttcggatt
    20641 agctgatgag gaagagtacg aataccaaga ttatcctgaa acaacaaaaa aggctgtaca
    20701 gccaactaaa aaaaatacaa cacagaatca atcaactcat tctgtatctg ctgcgaatac
    20761 tcatgaaaaa caaaaaacgg cgacttcacg tcctgtaacg catgaaagta ttcagccggc
    20821 tgaaaagaaa aaagtaattg aactacatca atcacctaca aaaactgtag cgaaagaaag
    20881 aacagcttca agaacctcta cacaacctaa agcaaatctt gctggaaaaa tcactgtaat
    20941 ggaaccaaga gcttattctg aagctatggc gattgctaaa cgaatcattg ctggagaagc
    21001 aaccttagtg aattttcatt tagtagaaga aaaacaagcg agaagaatcg ttgattttct
    21061 aacaggaaca gtttatgcac tagatggaga tatcaaacgt gttggagatg agatttttct
    21121 ttgtacaccg actgggatgg agattgacag ttcaaccgct caatcttttg cagatgcgaa
    21181 tttgtttgat ctgtaagagg aggaactacg atttatacta ttgcactatt acttcattta
    21241 cttcaacggg cagcctattt ctatagcatt atcttgatgg tatatgcatt gctctcttgg
    21301 tttccaggcg ggtatcaatc agggctagga cgattattag ctaagatctg cgaaccgtat
    21361 atcagtttgt tcgatcgtct gcctttgagg attggaccct tggatttcac aattttaggg
    21421 gcaatcctga tcttgaactt agccgtacaa ggtttagcac agatcgttat atggataact
    21481 gtccatttag tttattgatt gaagcaacaa aatctttatt ctcagaatag ataggagggg
    21541 aaaattttgg atgcaaatgt ataccagcac tttcgggcag atgaacgtac atttatcgat
    21601 tcggtaggcg attggattga acaggttcaa ggacaatatg caccctatct gaccgaattt
    21661 cttgacccaa gacaatccta tattttagaa acactgattc gacaaagcag tgatttgcgt
    21721 ttcatgtttt atggcggata tgaacaggca gaaagaaaaa gatgtttgat tttccccgat
    21781 tattacgaac ctgtagagga agattttgaa acagcattgt atgaaatcca ttatcctagc
    21841 aaatttgcca gcctaagtca tgggaaaatc cttggaactt tagtgggaac ggggatcaaa
    21901 cgctcttact ttggcgatat catctcggac ggtgatcgct ggcaagtatt tattgcaaat
    21961 gaggtggatc attttgttgc tgcacaagta acaaagattg gaaaggttgc tgtacgcttg
    22021 gaaccaatca aatacacaga aatgattatg ccgaaagata gctggacaca agaaagagga
    22081 acagttagtt ccatgcgact agacagtgtg atctctgaga tttataatat ttccagacag
    22141 cggtccaagc agctgatcga agctggtaaa gtaaaagtga actggacaga aaatacaaga
    22201 cctgattttg tacttgaact attagatatt atttcagtca gaggttatgg cagagtccag
    22261 atccaagaac ttgaggggaa gacgaaaaaa gaaaagtaca gggtactgtt cggtgtatta
    22321 cgtaaataaa ataaaaaggt ggtttactca tggctttaac tccattagat atccaaaata
    22381 agacattccc gacaaaaatg cggggatata atcaagacga agtagatgat ttcttagatt
    22441 tagttgttcg tgactatgag gaattgactc aaagaaatcg agaattagaa aaagcagtca
    22501 aacattctga agaaaaactt gagtacttca atgaattaaa agatgctttg aaccaatcaa
    22561 tcatcgtagc tcaagatacg gctgataaag taaaaacaag tgcaagcaag gaatccgaag
    22621 tgatcgttac ttctgcacaa aacaaagcag atgagttagt tgcgaacgct gaaaaacgtg
    22681 cacatcaatt aacaactgat gcagaagaaa aagcaagaaa aatcttgaca gatgcaacag
    22741 aaaaagcaag acagcttgcg acagaaactg aagatcttaa gaaaaaaaca cgagtattcc
    22801 atcaacgtat cagcttgatg cttgagtctc aattagaaca agtgaaaagt ccggaatggg
    22861 atgaaatttt acaaccattc tccagctacg tgactgacag ccatgaagtg atcaaagaag
    22921 ttttagcaaa acagcttgac aatgaaaatg atactgatgt aaactcagac acaggagtca
    22981 tagaagcacc tatgacggca tttgatacac aggctattga tatctcgatc gtgcctggtg
    23041 aaaatgaaaa cgagtagaac agaaaagcac tttgttattc ttaaagcgag ccgatgagag
    23101 tggaagttcg gtaagacact gcatggtgaa agatcctcta cttgagacct gcttttccga
    23161 aattagagta agaagcagcg gctgacctcg ttattggtca cagagagttt gcactagata
    23221 gatgcaacta aatttgggtg gtaccacggc atgttcgtcc cttgcggctg aacatgcttt
    23281 tttgtgtccg gaaaacaaat aaaggagtgt atggttgaat gaaaatgaaa gaaaccttgc
    23341 aattaggaaa aacaaaattc cctatgcgtg gtaatttgcc aaaccgagaa ggcgagtggc
    23401 aaaaagaatg ggaagaaaat cgaatctacc aaaaaagaca agagttgaac gaaggaaaac
    23461 ctagctttgt tcttcatgat ggacctccat atgccaatgg taatatccat ttaggacatt
    23521 cattgaataa aatcagtaaa gacattatca ttcgttcaaa atcaatgtct ggctatcgtg
    23581 caccatatgt tccaggatgg gatacacatg gattgccgat tgaacaagta ttggctaata
    23641 aaggcgtgaa acgtaaagaa atgtccatgg cagaatactt ggaaaaatgt cgtgaatatg
    23701 cactttccca agtagataag caacgagcag atttcaaacg cctaggagtt gcaggagatt
    23761 gggaacatcc ttatgttaca ttagatcctt cttatgaagc tgcacaagta cgcgtatttg
    23821 gtaaaatggc tgaaaaaggc tatatttaca aaggattgaa accaatttac tggtctccgt
    23881 caagtgagtc ttctttagcg gaagctgaaa ttgaatataa agatgtaaaa tctccatcta
    23941 tttttgtagc tttcaatgtc aaagacggca aagatattct tgatgaagat acagcttttg
    24001 tcatttggac gacaacacca tggacattgc cagcaaacca aggtattgct gtaaatccaa
    24061 aatttacgta tgtattggtg gaagccgatg gccgtaagtt tgtcgtagcg aaagatttgc
    24121 tagaaacagt ccaatcagaa atcggttggg aaaatgtaac agttctaaaa gaatttgctg
    24181 gacaagaaat ggaatatatg actgcaagtc atccattcta tgaacgtgaa tcacttgtga
    24241 ttttaggcga tcatgttact ttagacgctg gtactggatt agtccatact gctccaggac
    24301 atggggaaga tgactatatc gctggtagca aatataaact atctgtagtg tcacctgtag
    24361 actcaaaagg attgtttaca gaagaagcac ctggatttga aggtatcttt tatgacaaag
    24421 caaatcctat gatcactgat ctattaaaag aaaaaggagc tttgctgaag ctagatttct
    24481 ttactcatag ttatcctcac gattggcgga cgaaaaagcc tgtgatctat cgggcaactc
    24541 cacaatggtt tgcatctatc gataaattcc gtcagaatat tttggatgaa gtagaaaaag
    24601 tagagtgggt tatcccatgg ggtaaaacac gtttgtataa tatgatccgt gaccgcggcg
    24661 attgggtaat ttcccgtcag cgtgcatggg gagtaccact tccgattttc tatgctgaaa
    24721 atggagaagc aatcatcaca ccagaaacaa tcgaacatgt ggcaaaatta tttgctgaac
    24781 atggatcaat tatctggttt gaaaaagaag cgaaagaact tcttcctgaa ggatttaccc
    24841 acccaggttc tcctaatggc gaatttacaa aagaaaaaga tatcatggat gtctggtttg
    24901 attctggttc ttctcatgaa gctgttttac gtcagcgacc agaattatca ttcccagcag
    24961 atatgtattt agaaggatca gaccaatatc gtggctggtt caactcaagt atcacgacta
    25021 gtgtggctat caatggtgta gcaccataca aatcagttat ttctcaagga tttgctttag
    25081 atggtgaagg ccgtaagatg agtaaatctt taggaaacat cattgcacca gaaaaagtga
    25141 tcaaacaatt tggtgcagat atccttcgct tatgggtatc tagtgtagat tatgaagcag
    25201 atgttcgtgt ttctatggat atcttaagtc aagtggcaga agtatatcgt aaaatcagaa
    25261 atacgatgcg tttcttatta gcgaatacag aagatttcga tcctaaaaaa gatactgttt
    25321 cgtacaatga tttacgttct gtagacaaat atatgaccgt tcgattgaac caagtaatca
    25381 aagaaatccg agaagaaggg tacgacaaat ataacttcat gcatatttac cgtacagtta
    25441 tgaacttctt gacggttgat ctatcgtcat tctacctgga ttttgctaaa gatgtagttt
    25501 atattgaagc agaagatgat tatcaacgtc gttgcatgca aactgttttc tatcaaacag
    25561 ctgtagcttt gacaaaatta ttgactccta tcattccgca tacagcagaa gaaatctggt
    25621 cattcctgaa agaagaagag gagtacgttc agttaagcga gtttcctggt tatgaagagt
    25681 ttgcaaacca agaagaattg atggatacct ggactgcttt catggacttc cgtgataatg
    25741 tcttaaaagc attggaagaa gcaagaaaca gcaaattgat tggtaaatct ttggaagcaa
    25801 aactaactgt ttatccaaaa caacaagttc gtgaaatgtt aaaagctttg gatgcagata
    25861 ttgcacaatt attgatcgtt tcaccagact actttgaagt tatggcagca gatgaacaag
    25921 tgccagataa cgctatggta tttgatgatg ttgctattac agtggaaaaa gcagacgggg
    25981 aaacttgcga tcgctgtcgc caagttcgta aagatgtagg agtagatgaa aaacttcctc
    26041 atctatgcgg tcgttgtgca aaaatcgttg aagacttcta tcctgaagct gtagctgaag
    26101 gattcgaaga aaagtaaaag ggaaaagggg gtgtgacatg ttctttgtca cacccccttt
    26161 tgttatcaat cgtttaattt tccacgttca cgataccagt tatctggttg ccagatccat
    26221 tcgaatccgt ctttttcaag tagttcgaaa gctgctttag gtcccattga tccagctttg
    26281 tagttgggaa aatccggttg tgtttggtcc caagcttttc gaatgacatc cacgatatgc
    26341 caagattgag cgacttcttc ccaatgagag aagttcgttc catcaccatt caacgcatct
    26401 aatagtaatt tttcgtaagc ttctgggcta ttttcaacca tttctgcact atttctaaat
    26461 tccaatttga ttggttccgt ttcaaatcct tgccctactt ctttcccatt caaggataaa
    26521 gaaaatcctt cagttggctg aatatagatc gttagaacat taggtggaag tgtcgtatcg
    26581 tcacatggtt catcgacaga tgttttgaat acattaacag gaacttgttt gaatacgatg
    26641 ttgatccgag tccctttttc agtcaaacgt ttgcctgtac gtacatagaa aggaacacct
    26701 gaccaacgga agttatcgat agtgaatttg cctgcgacga atgtttcagt ctctgattga
    26761 gcatccactt tgtcttcttc gcgatagccc ttgaattgta caccatccaa ttcaccttga
    26821 gcatattgcc cgcgaacgaa gttttctaac acttcttctt cgttataaat gcggatagct
    26881 ttcaatgctt tgatcttttc tgtacggatt tctttttcgg aaaatgctac tggcggttcc
    26941 attgcaagta atgctacaac ctgtaaaata tgattttgca ccatgtcttt aagtgcgcca
    27001 ctatgatcgt agtagccccc gcgatcttct acaccgaggt tttcgccaaa agtaatttgg
    27061 atattgtcga tatagcgatt gttccattga gcctcaaaaa tattattggc aaaacggata
    27121 gctgagatgt tttggatcat ttcttttcct aaataatgat caatcctgaa aatatcttgt
    27181 tctgggaata cttctctgat ttgctggttc aattcaaaag cagactggta atcagaaccg
    27241 aacggttttt ctatgatcaa acgattgaag ccattttttg tcataatatt ttgtgatttc
    27301 aaatgagaaa cgatcgttcc aaaaaattga ggtgccatag ccaaataata gacacggtta
    27361 ccgtttaatt gatagtgtcc atctaatcgg tcagacaatt cttttaatgt ctgataatgc
    27421 gttgaatcat ttacatcatg ggattgatag taaaaatggc ttgcaaaggt agttgcttct
    27481 tcatctgaag gattcaatgc acggattgtt tctttgacaa cttcacggta gtgctcatcg
    27541 ctccagggac ggcgagcggt tccgattaca gcaaaatgct cgcctaaatt tccttttctg
    27601 taaaggcgga acaaagacgg gtacaattta cgctgggcta ggtcacctgt accgccgaat
    27661 atcgtaaaca atacattttt ttcattagac atgtggatac actcctttat attttatcga
    27721 caaaaatcgt gctggccgcc ttaaagctga cggggatttt ctttctatca ttttggatag
    27781 tgatcggtcc ttcataagct gcgatgtcaa cgatctcata ttcttcgttg atattcaggt
    27841 caatagtgac taagtaatcc aacaattctt tttcatcaag cactcgtgcg atacgcactt
    27901 ttgtaccgac aggataggac tccaatgttt gtcggcgttc ttcatggaca acttcctctt
    27961 ttttaggaat cgatcctcca tgaggacaat gttcaggatg attcaaatag ttttctaaat
    28021 gatctgcaag atgttccgaa gtcacatgtt ccaatacttc agcatcatca tggacttcat
    28081 tccagctata gtctaaatgt tcaactaaaa aaacttccca taagcgatgt ttgcgaatca
    28141 gactgctagc tttttgcagt ccgcttgcag tcaattgtac accttgataa ggggaatgtt
    28201 ctacaagctc ttctttcaca agtttagaaa tcatttcact gacagatgca gctgagacat
    28261 ctagactaga tacgatttgt ttattgttga ctttcgttgt atctccacct agttctaaaa
    28321 tgattttcaa ataatcttct cgatttggtg tcatattcga tcatcctttc atggtaaaaa
    28381 accatcatta gtaagtgtaa caaaaaaaac gttaattgac tatttttcta aataaaaaac
    28441 aaaggatttt tagacgtttt tacaacaaaa aacgaaagat gatagagaag actcattctt
    28501 tcgtttataa aggatatata tatacattgg gagggatcta tttttcagat aacaacttat
    28561 atgaagtgtc tgcagattcg tcataaagag tttgttcggg ataagaaaag ataagataaa
    28621 aaccatactg atcacgtacg actaaacgat attcatctat ttctaccaca gatgtgccaa
    28681 gagactgacc gttgatcaaa aacgtgtaat cagatgtaat ctgtaattgt tgtttttgct
    28741 ggctatcact aaagaaccat gtaccttgat actttgagag tccttctgac ttttccgctt
    28801 gttttttctt tttcaagtaa ataaaacttg agagaacggt aatgagtgaa ccaagaaaat
    28861 agatcatttt ttttatcacc aaatcactcc tttatgagaa aataatcgca tagacatatt
    28921 ctttcttctt tatttcattg taccgaataa aagctaaaat ttctctaaga ctttcgactg
    28981 gtttttttac tgtttttcta caaattatga taggatacat agtaggaaga aaggtgacat
    29041 aatttgaagt atcagcaagt ggaaatagct ttttttattg accgcccgaa tcggtttata
    29101 gcacattgta tgaaaaaaga cggagaaata attattgcgc atgtcaaaaa tacaggtaga
    29161 ggaaaagagg tcctgatacc aggcgctgaa gtggctattg tatttgctcc tggacctaaa
    29221 cggaaaaccg cctatgatct gattgctgta aaaaagaagg gtgactggtt taatatcgat
    29281 agtcaattgc ctaatcggct ggcaattgat ggtattttag acggcacgat ccttttacca
    29341 ggattaactg gtccatttac ggtatacaag cgtgaagtaa cttatcttca ctccaaattt
    29401 gatatttatt tagagacggt ggtcggacaa aaagcatttg ttgaagtcaa aggtatgaca
    29461 cttgaaaata aagcgattgg tgcttttcct gatgcgccca cactcagagg attgaaacat
    29521 ataggagaat taacgtatgc cgcacaagat ggttatgccg cttatgtctt gtttattgct
    29581 cagttcgaac atcttcatct ggcaacgatc catgaagaaa tgcaaccagc actagctgat
    29641 atggttcgac atgcgcagca aagcggagtt caaatcctcg cctataattg tcaagtatcg
    29701 gcagatgagg taactgtcaa gcaggccatt ccatttgatg tagatatccc ttttgaagat
    29761 cctatacaat agaaagaagg taaaataatg atcaacttag gtattatcgg aacaaattgg
    29821 attactcatc aatttgtcca agcagcttta agcacaaaaa aatatgaatt gacagcggta
    29881 tattctagac atttggaaaa agcacaggaa tttggttctc attatgaagg ggatatcgaa
    29941 ttcgcaacag atttgaagac ttttttcaat attactcata tggacaccat ttatattgct
    30001 tctccgaata gtcttcactt tagtcaagct aagcaagcaa ttttggcagg aaaaaatgtg
    30061 atagttgaaa agccagcttt ttcgacgcca aaagaaatgg atgaaatcat ccatttggca
    30121 aatgaacaca atgtgtattt ttttgaagca gcaagaaata tccatgaatc aggttttaaa
    30181 aagattgccg attacttacc attaaaagac gagataatcg gtgcaaattt cagctatatg
    30241 aaatattctt cgcgctatga ccaagtatta gatggagaag aaccgaatat cttctcgact
    30301 catttctcag gtggtgcatt gatggattta ggtgtttacc ttgtatatgc agctgttgct
    30361 tggtttggaa tgccaaaaga agtccattat ttcccaagaa agatttctac tggtgtagat
    30421 ggtcttggat gggggatctt acgttatgag aattttgatg tatcgatcca gccaggtaaa
    30481 atcggtgatt cttatttacc gtcagagatt tattttgact caggaacgct agtgatgaat
    30541 ggagtcaatg caattgaaca agcacaattt catgatcgcg cgcataaaga aatcattgat
    30601 ttggcggtaa cagctgcaga aaatccaatg gcagatgaag cagaggattt tgccaatgtc
    30661 atggctcatc cgaaagatcc agcttgggga ttgttgtatg aagagtggat cgagctagct
    30721 agaaatgtga accaagtgat ttatgattta agaaaaaatg gcggcatcaa attcgatgca
    30781 gataatgagg aagattttta atgaacgaat ttgtacaagt atttcccgaa aattggcaga
    30841 aaaaatatga ggataaaggg tttatctcac cttctcttat tcaacgagca gtttttcagc
    30901 cactaagtaa taaacaaagt atagtagcgg tttctcctac aggttcaggt aaaacactgg
    30961 cttatctttg gcctctacta ctaaacgtgg aaccaggtga agcaagtgcg ttggtcattt
    31021 ttgcttcttc tcaagaacta gctattcaag tagcagatgt tgcacgtgaa tggggaaaag
    31081 acaaagagtt gaaagtccaa tctttggtgg gcggagccaa cgtcaaacgt cagattgagg
    31141 gtttgaagga aaaaccagaa atcttgattg gtacaccagg aagaatattg gaattgatga
    31201 aagcaaaaaa aataaaagca catcaagtga aaacaatggt atttgatgaa gcagaccagc
    31261 tatttgacgc tgggaacagc caaatcattg atcaaattct tcatcaagct ccaacagagt
    31321 atcagctagc ttttttttca gcgacagcag atcgttccct tgaaagcatt gaaaaaatca
    31381 ctgggaaaac gtttgagaca attgacgtga cagcagagga cgattctcga aaaggactga
    31441 gacattactt tttacgtgta ccgatacgaa agaaagatga ctacttgaga cgtctcacgc
    31501 atatagaaga ttttcatggt ctgattttct ttaatcaact gactgaatta ggcgtaatgg
    31561 aagaaaaatt gctgtataga ggtgtaccgg taggaagtct ggcctctgat cagaacaagc
    31621 tactccgaaa agcagcgcta gaacaattca aaaagaaaat ggtcagagca cttttgacca
    31681 cagatgtggc ggctagaggt ttagatatta caggtcttcc ttatgtagtg aatgcagaag
    31741 ttccgttaag tgaagaagct tatttgcatc gttcgggaag aactggtcga atgggtaatg
    31801 aaggaatagt aattacactc gtacaagata ataatctaag agatttaaaa cggcttttac
    31861 gtccatcaaa tataacactt gaagagattt ttttatacgg aggtaagctg caaactgagc
    31921 agccaaccaa agaagatgca gctcctgcag taaataaaaa gtatgcgaaa actttttctg
    31981 gaaaggagca gaacccagaa tataacaaag aaaaaataag aaaaaaagtt aaaaataaat
    32041 caaaaaaaga gagaaataaa ggagcaaggc gcaaataagc gcctttttat gctatgatta
    32101 tgaaaacggg aacaaaattg gttgccagag gaggggttat gtgaaagaac gtcaagaaga
    32161 gatcttgcag ctattgacag aaaaccaaca aggattgaca gcaagtgatg tagctgagcg
    32221 cttaactatc gatcgaagca atgcaagccg atatttaagt gaactataca aggcccatca
    32281 tattgtaaaa accgctggcc gaccagtcgt ttactctttg ccaacggaaa agtcaaaatc
    32341 tgatgaagtg catgtcgact cttctacgca agtgactttt gaaacactcg ttggagaaaa
    32401 tgattcttta aaagtcagca tacagcaggc aaaagctgcg attctttatc caccgcgggg
    32461 gttgcatacg attatttttg gagaaactgg tacagggaag tcgatgtttg ctgaatgtat
    32521 gtatcatttt gcaatcgatt cagagatgct ttctgcagat gcgccatttg tttctttcaa
    32581 ttgtgcggat tatgctcaaa atccgcaact gctctttgga cacatctttg ggatcaaaaa
    32641 aggggcttac acaggagcag ctcaagatag tcccggtcta atagccaaag cagacggcgg
    32701 tatattgttt ttagatgaga tccaccgttt accgccggaa gggcaggaaa tgctgtttac
    32761 tttcattgat aaaggtattt accgtccgct tggagaaagt gcacaagtac atgaagcatc
    32821 tgttcagatc attggggcga cgactgaatc ttcagaaagt ttcttatcta cgttcaatcg
    32881 acggattcct atggcaatca ccttgccgaa tcttgcttca cgttcactgg atgagcgtta
    32941 tgaaatcatc tctcttttta tcaaacaaga agcgaatcgt ttgaatcaac gtatcgatat
    33001 cgaaaaagaa gcgattcttg ccttcatgct ttatgatgcg gaaggaaata tcggtcaagt
    33061 gaaacgagac ttgaaactag tctgtgcaaa atctttttta cattaccgta ctcatcatga
    33121 gaaaaagcta atcattcgaa aagaagaact ttcccttcaa gtacaaaaag gattactgaa
    33181 aatcaaggaa ataccggaac gtctcgaccg attcatggac agcaaaagtc agtatttgac
    33241 atttgaacca ggatttgctg atgttgtctg gtcacaagat ccagaacgaa acatgcaggt
    33301 ctataacgat attgaggaaa aagtcacttc tctttccgaa accggtgtgg agaacattga
    33361 tctagagacc ctcatttcaa aagatgtgga tgcttatttc cagacatatg tcaaagaatt
    33421 gactaaaagt tcgattccca aagatctttt gccagatgaa atctggcggt taaccaatca
    33481 attgtatgat gtggctgaaa aacggttaga ccgtatttat aatgaaaaag ctcgttttgc
    33541 ttttgctttg catctgcaaa gtacattgga tcgtgtgaga gaaggtcata tgatcgtcca
    33601 cccagatttg aataacgtac ggaaaaattt taagagggaa ttccaggtag caatcgatct
    33661 ttcaacgatc attgaagaag aagaacaagt tgagattcct tttgatgaaa tcggctttat
    33721 tagtatgttt ctttctatca atctaggaga aagtgagcct gttaaagaaa acaaagtaga
    33781 tgttctcata ttgatgcacg gcagacagac tgcttcaagc atgttggaga tggcacaaga
    33841 actattagga acaaaagtag ggaaagcttt taatatgcca ctggttatgg aagttcaaac
    33901 aatgtatgaa gaagtgttac aatatgtcaa aaactatcaa gagcatttga cgaatggcct
    33961 actcttgtta acagatatgg gatcattaaa caccttcagt gatttgattt atgaagagac
    34021 tggcattcgg acgaaagcta tcacgatgac aagtaccatg attgtgatag aagcgatacg
    34081 gatggcaagt gttggaagaa gcttagaaga tatttatcaa aatatccagc tttcctttga
    34141 atcgatcgta cgtgatcagt tcaaagcgga aaagtctgaa actttacagc gtaaaaaagc
    34201 cgtaatcgtg acatgcttta ctggtgaagg agtagctgcc aaattatatc agcgtattat
    34261 gccagtgatt aatcaaaata aggtagaact gatccagatg cagtttttag aacgtgaatc
    34321 attcaaaaaa cacatcgatg ccttattgga agaatatgag atcaaagcaa ttgcgggaac
    34381 agttgatgtt gattatcaaa atatcccatt tttttcagcc tatgacgttt ttgatgatga
    34441 aaaactaaat attttaaagc gtatagctga tgatgagatc ccgacagcta aaattgtcga
    34501 ttcacttgct ggatcattaa gcgatgtctt ttctattgat gaactgatcc gttcattgca
    34561 aaagacagtt cagcagatcc agaccgattt gtcgattatc gtggaacctg gagtagatac
    34621 tggaatcgtt atccatttag ccttcatgtt agacggattg atcaaacaag aaagccatcg
    34681 gcaatttgag aaactgtcta ttttctcaaa aatttatcgt ttggaaatgg atatcgttcg
    34741 ggcaaacttg atggcactag aacgaaaata tcaggtaaaa attccagaag atgatatcgc
    34801 atttcttaca caaatgtttc ttgaaaataa aataaaaaaa tgaacacact aatcatactg
    34861 tttacacact aaacatctgt gtgcgcacta taaaaaagag ctaaaaagca tgaaaacgct
    34921 atttcagctc ttttttttat tggcatgctt cttgcgttat ataaaagtgt aaaaaaataa
    34981 aaggagtgtt aaaaatggca aaaaaaacaa tcatgttagt ttgttcagca gggatgagta
    35041 cgagtttatt ggtcacaaaa atgcaaaaag cagcagaaga aaaagggatg gaagccgata
    35101 tttttgcggt ctctgcctca gatgcagata ataatttaga atctaagaat gtcgatgtat
    35161 tattattagg tcctcaagta cgtttcatga aagctcaatt tgaacaaaaa cttgcgccta
    35221 aaggtattcc attagatgtc atcaatatgc aagactatgg catgatgaac ggagaaaaag
    35281 ttcttgcaca agcagaaaat ctaatgaaat aaatcaagta tgcagttggg ggtataacaa
    35341 cgtggaagat caacaaaatc tagaagccat catggggtta atcatgtttg gcggtaatgc
    35401 aaagagcgat gcaatggaag cgattgcggc tgctaaaatc ggtgattttg agttagcgga
    35461 taggaaaatc gctgatgcag aagaatcact tatacaagcg catcatgcac aaacagacat
    35521 gttgacacaa gaagcacaag gaaatcatat gcaagtgaca ttattgactg ttcacagtca
    35581 agatcactta atgacatcta ttgcgtttac tgatttagca aaagaaatca tcgaacttta
    35641 tcgtcgcgta tacagcgaat aaatacaact tcttttttga acggattttt tattttgtta
    35701 taaggagaac tctctttata taaaaaactt ataggaggaa gtaaaaaatg aacggactat
    35761 caagttggat ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt
    35821 tagttgcttt gcgtgatgct tttatcggca cactgcccgc aacaatggcg ggatcagtcg
    35881 ctgtaatgat caatgcaatt attcgtgatt taccgcaaca gtttaacagt acctatgctg
    35941 acagtttagc agcagacaaa ggaatctttg ccgtattgaa atgtcatcat cggaatcaat
    36001 ggttttgtct ggaacggtac actagcaatc gccggattga tctttgcctt ttcttggggg
    36061 tataatcttg cccgagctta tggagtaaat gatctagctg gggggatcgt tggtttagct
    36121 accttgattc aaggtatttc ctttggtcca gcattatcag cagcattaga tgtgaatgtt
    36181 ccttcaaatg tcgcagatgc aatcaatagt tctgagattg gtgcaacgat tgcagatggc
    36241 acactgtcag ttggattatg gggttggcta aaactagatc atttgaatgg aaacgcttat
    36301 tttacagtta tgatcatggg agctatttca gtcattattt tttgtaaatt gatgcttgcc
    36361 aacattacga tcaaaatgcc tgattcagta ccaccagcag tatctaaagc atttgcagcc
    36421 attttaccag cgacgatcgc tttatacgtc attgctatta tcaattatat cgtaggtcgt
    36481 gtagctgatg ggcaactgat cattgatttg gtgcaaaaat atattgcaga accattccta
    36541 ggattatctc aagggttggg agcagtattg atcgtcacag tatttgttca agtattctgg
    36601 ttctttggta ttcatggacc aaatgtattg gcaccagtgc tagagggaat ctggggacaa
    36661 gcacaactaa tcaatattga tattttccaa aaaggttata acggaaaaac tggtacacca
    36721 gccgtgttag atgcaatcga agatggaaaa gcttatatgt gggtccgcgg atcgttcgat
    36781 gcttttgcat ggtttggcgg atcaggcgga acgatcgtct tactgatagc tatcttgatt
    36841 ttctctaaac gtgcagatta tctaacagta gctaaattat ccattgggcc tggtatcttc
    36901 aatatcaacg aaccagtgat gtttggtctg ccaatcgtat tgaatgcaat catgtttatc
    36961 ccattcttga ttgctccagt agtagcaaca gcaattggat ggacagctac ttatcttggc
    37021 ctagtcgctc cggtatcgca gcaagtcaca tgggtagtac cgccgttctt attgtcattc
    37081 ttagcaacag gagcggattg gcgggcaccg atcgtaacat tagtatgtat ggtcgtgact
    37141 ttcttgattt gggcaccatt tgtcatctct gcaaacaaaa tggaccctgc cgcaggcgaa
    37201 caacaataag atgaacgtga ggagcgggac gctttaagtt tcgttcctca taatgaattt
    37261 tcgaggagga actgaaatga ttacaattgc cagtgcaatg caacaagatg aaatcaaaga
    37321 attgttagaa ggatacaatg aagaaggaat gacttttaca tttaaagaaa aacaaggaat
    37381 caagctgttc tttgaaacaa gtgcagaaga tccggaagtt gcggctaaaa aagcaaagga
    37441 actaatcaaa gcacagcctt ggggaacagt cctttatttt caatcaacag ctgtttagaa
    37501 aatggtttaa aaagatagga tggttgagat gataaaaaac ggtttatttt tatgtagtat
    37561 tggttctttg atcgtgttgt atagtttgaa tccacattcg caaaggtatg atttgtatag
    37621 tatggtcaca ggtctttttc taacaggatt aggaatctat ttcttcatga aggggaaaaa
    37681 aagagaagaa gcaaaggaga aaaataacta atggaaatca cgaaaaaatt aaatattcca
    37741 gcatcgtttt tttacgataa agttatggac tctgtccttt tcgatgtacg taaagcgacc
    37801 ggcaaatcag taactagaaa acagctgaaa aatttggagt acgtcaaaca attctcgcaa
    37861 aataaccggg ctcgcataaa aatcgaagaa gtcattgaaa accagtctta taagtttcgt
    37921 acatcgacaa cgaagaatga attcgtcgtt cattatcaaa tcaaaccatt ggacgattcg
    37981 acctgtgagg ttcattacac agaaaaaatg gagtcttatg gcatgcttca aaagttgaat
    38041 gatatgcttt taggaacaat cttgatgtat tttaaacgga gacgtttcaa aaaaatgcta
    38101 gatatgatcg aagcaacgta ttaaatcaat gaaacatggt actatttagt tattgaaaaa
    38161 tgaagtaagc tagaaggaat ttttcatagg aagatagatc tttcctgcga atcctcacta
    38221 gcttttttgt aggaaggggc agcaaaatga caaacaaagt aagagagcta ggtgtatctt
    38281 cgattgagga aatgtttgat ttagccactt atgcatttaa tggggcagat acacctgaaa
    38341 ggcgagaacg ttttcgtaca cttgcggaga attcttggaa ttacggattt tttgatgagg
    38401 aggatcggct aacaagtcaa gtcatggcga ccccttttcc agtgaatttt tacggacaag
    38461 agtacctaat ggccggcatt ggctatgttg cttcttatcc agaagcaagg ggacaaggcg
    38521 ggatcaatca gatcatggaa aagatccttg aagattgccg tgatcgtaaa gtaagcttat
    38581 cttatctggc gccattctcc tatcccttct atcggcgcta tggctatgaa caatcatttg
    38641 ataaaatcag ttatcaacta aacagtcgtg atgtgccgtt tatcaaaaaa aaatctggaa
    38701 aaataaagcg tatggatttt gaagatgcaa aagctgcaat cagaagaatt tatgaacaga
    38761 tgccggaaaa ccaacgagct ggtctgaagc gcgaagactg gtggtatacg tataaattca
    38821 agcaaaaagg caattaccag tttgctttgc attttgatga taatgaggaa gcaacgggat
    38881 atatcgttta tcagcttgca acgtcttcct ttattattgc ggagtgggga tttttggatc
    38941 atgatgcttt ttgcagcctt gttcgcttcg taagttctca caatggtgct tttgaaacat
    39001 ttgaatattc atgcggctac ggcggtaatg atcaaaatta tctattagaa aatccatttg
    39061 cttctgtaat gatcacacct tatatgatgg cgaggatcgt agatatttct ttgtttttgg
    39121 aaaattatcc atttaaaaag agacaagctg catttgccct acaaatagaa gaagatcatt
    39181 atgcgaaatg gaatgaagga atcgtggaag taacaatcga aaatggaaaa aatcatctct
    39241 caaaagtaga acaaacaaaa ctcccaatcg ttcgaggaag tatccaacat atcacgcagc
    39301 tattattagg gtatcgtaaa gtggaagagt tggtattcca agaaagaata caggtgacaa
    39361 aagaatatga agaaacgtta agctcaatct tgccacagca acagccaatt cttaatgact
    39421 atttttgata aaaaacgggt gatgacaata tttttgtcac cacccttttg tttttcattt
    39481 ggcatttgaa ggaaaatgtt aaagtaaatc ctcttttttc gtaaatttga attttttgtt
    39541 ttcttttgtt ttagggtttt cttttttgtg gtcttggatt gcttctttcg taatcttttc
    39601 ccgatttttt ttagatgaat ctaagcgttc tgcgtatact ttttcttttg gcttatcatt
    39661 cattggaaag gacctcccta cattatttca ttctacctct agtttatcat ctgttaagga
    39721 ctagtggaaa cgttttgcct aacttataag ctgatttttt tagcagataa gcttgcaagt
    39781 aaaaaacaaa caagctacaa ttgatttatt gaaattgttc ctgtagctca gtaggataga
    39841 gcaatcgcct cctaagcgat aggtcgctgg ttcaattcca gtcaggaacg tataaataac
    39901 ccctgtatcc tttgcggtac aggggtttgt tttgtatttg tgccaaaaat gtgccaaact
    39961 agttatcatt tttatataaa ctatcgaaca cagtattgat tttttcttta tcctgatctt
    40021 caagctctcg aacaatatga agataagtag acattgtagt ttctaaactg ctatgtccta
    40081 aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat gcatgtgtat
    40141 gccttagtcc atgcaaagta aattgctttt caatccctaa ttcttttaat tttttgcgca
    40201 aatatttgct gacggcgttt gatgaaacta atccattttt taagttgaaa aaaacaaaat
    40261 tatgagggtt tttgatttca aaattctcga ataattcttt ttgattgatt ttaaactttt
    40321 ttaagagatc aatcgtatgt ttatcaatgg aaattttcct atttgaagtt tcgttttttg
    40381 tttttcccca ttcatttaat ttataattcc atgttttatt aatagtgatg atttgatctt
    40441 cgaaatcgat atcttcccat gttaatccta gaagttcagc aaatcgcatt ccagtggcac
    40501 cagcaactaa cacgagcata ggagaagagt actgcgcaga aaggttttct tctacgagtt
    40561 tcattaaaga tttaaattga tcataatcta aatatttatc ttcttttggc ttcaatgaat
    40621 cttttccttt gattactgct tttctagttg gatcaaacgg aattaatcct tcttctacgg
    40681 catctttcaa cgacgctcta atatggttat taaacttaat aacggtagat tttacatggt
    40741 ctttagcata cttatttaaa aagcgttgat atcccactct atctaaatca gaaatcaata
    40801 ccgctggcat atatttcttt atgttcatta acgtatcttc gtattttcta taagtgatag
    40861 gagatactgt tccttcttta taaagttgca tccaatcctc aaagtaatct gataataata
    40921 gatttttgcg atccccttta aggcctttgt tcaagttata ttctaattcg ttggcggcat
    40981 cttttgcttc tccttttgtc ttaaatcctg attttcttat cttcgtgtat tttccgtcat
    41041 cttttttgta tgaaatttcg tattgccaac tatttccacg tttgactaat cttgccataa
    41101 ttgaatcagc tctctttctc tgatacaata ggaactataa agaagcccat tgtttgggtt
    41161 ttattatttt ttgcacatcc tttttgcttg ccggcgcaag gatgtgctat ttgtcgtttt
    41221 aatggtataa ttggatgtgt attagatcat ccaatcagag gtgtttatta tgattaaagt
    41281 gatttttaaa agaataactg aacttccaga gtttagatta tttgttgatt attggtatgt
    41341 ctggtttttt attgttgcat tgctgatagg tctagatgtc atattagaaa gaaaacttaa
    41401 gtaagagttg agttaataaa ttgaattcct aaatgagata ttttgtagcc catattttgt
    41461 ttgccgacat cacctaatac tatggagcta atcgtttttt ctaacagacc aaaatcagat
    41521 aatctattta agctaccatc tattagctct tgtgaatagc cagacatgct gatatttcca
    41581 gtgaaatttt cgtttccacg actcattaag catgctaaca attgaatctc aatcgatgtt
    41641 aattgattca gtacaagaag ataatactcc tcgttatccc atgaagcgtt gttaattcga
    41701 agtaaacaat tcttataaag atttttatac atatctattt tattttgtgc ctttgatttt
    41761 tctacctcgt catttatccc ttcgaatatt ccaatgagtt ggtccttgtt ctctaagttg
    41821 aaaacatctt ctggaatttg atcgcgaatg ttttctaatg attcgtttaa atttttgtag
    41881 aatttctcga tgcgtttgaa acgcttctcg tttttagacc caaaataaag tgactgcaca
    41941 acgccaccaa tataaggaat tgctccaata ccgccttcaa ctattaattc aagatattct
    42001 tttttggtta gtttttcatt gctattatcc atagttacct cctattttgt catttccttt
    42061 acaagcattg tgactcctga tggcactctt ttgtataggt tttattattg tgcacaatct
    42121 aactcttagt ggggcgtggt tcatttattt tttacttact ttactattaa tcatatccca
    42181 ttgagcatag gatttttcta tgttcgatcc tcttgaacta atttctcctg taaaagttac
    42241 tttatcgccc tttgtaaagg tatcagcatt gtttaagtcc ttcgcaaaaa ttacatatga
    42301 aactttaggt gttgcagaaa tatcattcca acttttatca tcgtactctt tgtctgctat
    42361 aatggctatt ctagtagtca ttgattcgat aacagttcct tcaaaagtat atgttttacc
    42421 actaatatat ttagtaaaag ctgtattttg ctcagtagct ggagtaatag aataataact
    42481 atctaagaaa gcaccccagt catgattatt tgtctctaaa aattcttcaa attgagttag
    42541 gcttttattg ctgctgttag atgtagtagt ttgagtatca gatgatgagc tttcctgttt
    42601 gttattgtca tttgcctcgt ttgatgagga aagcgagaaa cctacggtag taaaaataat
    42661 cccgataata aggagaatca taggtgtttt cttatttgtt tttttgataa atcttacaat
    42721 taacatgatt aaacctataa taaataaaac aataccagta aaaccaaata taccgtccat
    42781 taatagatcc tctttctctt ttgatattga atattataaa ccaagcaatt cttttttctt
    42841 aaaatcgaat tcttcttgat taataatccc ttcatctaac aactctttat actttttaat
    42901 ttcatctgca cttgaaattt gtgtttcttt ggaattatta tctaatatag attgttgctt
    42961 ctctatatat ttttttaaat tttccatttc attccaatat ttttttgtaa acataacggt
    43021 gttttcatct ttcgtggccg ctaatatacc tccgcgactt tcgtttccgc ctagcaaggt
    43081 aaattgtata taaccattac tcatacctgg tttttttaat tgtacggctg atatattttt
    43141 aaaggggata gttttttctc ctttaatacc ttgattaact aaatttataa atccttttct
    43201 aataattgaa atagaatcat cagttacttt aatcaaggtt tttcctggag attttattaa
    43261 aatctctttt tccatttttt cttcctcatt tctgtgatat gatttttttt gtagaagctc
    43321 atatatgagg aaagagcccg tgttgcagca cgggcttttt taatgttttg ctgaaatcgg
    43381 tttttttaat aattcttgat gtattcatac atattgcctt gcgtaagaat attttttctt
    43441 aaaatagcat tggcagacag cataacaagt gtatctgagc ttattctgaa tagaataata
    43501 ctccataaat ttttcgagat taaactgaga tttatcagtc agttcgttct caatatagat
    43561 atttaataaa attagaatag ctattttatc tgcttctgtt tcaaattttg agtgaaaagt
    43621 tgtagaagta tcgtacaaaa ctgaaaattc aaaatgagag gcgctgaaat gtgcaagttc
    43681 atgagataaa tgaaaggctt ctgcagtttc accgtataga ttttcattca aaaaaatgat
    43741 tctgggtttt ggatagtaga aaccaggctc tttcatctcc atataaacta ctttcaaatt
    43801 gtattcgctc aacatttctt tcaatttcaa atacatacaa accatcactc caactattca
    43861 ttttcctcta aagctttagc aattgcaatc gctttacgca ttgtctcctt agatatttct
    43921 tttccatcaa aagaaaaaac agtatcgtct tctgataaat ccacatgttt aggggtttct
    43981 ctttcttctc tacctagaag gtagtctaca gagacatcga aatagtcagc aatccgactt
    44041 aattcatctg aattaggagt attatttttc cactttgcta gataaccgtt agaatatcca
    44101 aaattaattt ctaactgccg tatagatatc tttttcctct tcgccaagtc ttttattatt
    44161 tcataggtat tcatcgataa aacaaccttt ctaaatgttt acaaaaaaag tttagaaaaa
    44221 tacgcagaaa taatttgact aattctgagt aatgagctat actatatctt gtaaacagat
    44281 ttaacaacta aaaagacaac aaaaaacact attgatatgt aaatgcagac cgccaagaaa
    44341 gctttaaaat caatgttttt atgtcttatt taattatgct cttagtatag agtattactc
    44401 agatactgtc aattgaattt agaaaaaagt tgttaaattt gtttacgaat ataaaagaaa
    44461 ggaatgagaa atatgaacac accgcaaatt tttaatttcg aacaaaacga agttcggaca
    44521 attttagtaa atgatgaacc atattttgta ggcaaagacg ttgcgagtgt tttgggttat
    44581 tcaaacacta aagatgcttt gtcgcggcat gtagatttgg aagataagat ggggtcgcga
    44641 atcacgacct caggtcaatc aagagagatg acaatcatca acgaatctgg tttgtacagt
    44701 ttaatcttaa aatcaaaact tccctctgcc aaaaaattta aacgttgggt aacaagtgaa
    44761 gtgttgccag caattagaaa acatggaggt tatctaactc cagaaaaagt agaagaagct
    44821 ttgcttaatc cagatacaat cattcaatta gcaactcaac taaaagaaga aagaactgga
    44881 agattaatcg cagaacaaaa gattgcagag tacgaaccta aaatctccta tttagatagc
    44941 atattatctt ctacagattc agtaacaatt agtcagattg cagcagatta tgggatgtct
    45001 ccacaacaga tgaataaatt acttcataaa ctaggtattc agaaaaaagt cggtaaccaa
    45061 tggttattgt gcaaaaaaca catgaaccaa ggatacacaa aatctcatac aactgagatc
    45121 ccgaaagccg atggtggcac taaaattgta atgaatacca aatggacaca gaaagggcgt
    45181 ctatttatct acgaattact aaaaaaagaa ggatattacc ctcaaatgga tttagaggaa
    45241 attggttaga aaggagtttt agtatgactg acattgcaga aatcactcaa cgagatagag
    45301 aaaaaatcaa agaatatgtc gaaagttcaa agttcttaac ttacaccatg cttgctgaaa
    45361 gatttggaat tagcaaaagc tacttatctt taattttaaa cggtaaaaag acttctgcag
    45421 aagcaaacag aattatagat tcgattatca ctatgtacga attgtaaagg gaggaacagc
    45481 taatgcaata tctagaagca aaaatcccaa ttccagaagg ctatgtaatt atctcccaag
    45541 tggattatga ggagttaaaa aaagctgatg atactggtag atggatgacg ttgccagaag
    45601 tgctagaacg gattaacaga aaatatgatt ggtttacttc tagagtttta aagaacccaa
    45661 gatatagaaa tattatcgat atcgagaaaa acaaaaatgg atttgtctat tatccagttg
    45721 aaggaagaga cacatatcta tttttaagaa gtaaaacact tgaattttta gaaacaaatt
    45781 tttcggaaat cttaaggagg caagcggatg ggaaaattta atagagcgct agtgttcagc
    45841 gcaccgctaa tcatctacgc tttaggactt tggggaagca ggcaagcgtt gataggaacg
    45901 attgtttaca tggtttggat ttttatgggg cttgatgaag ctgaagctga gtacagagcg
    45961 aaaaagccaa ccgagggggc tgactaaaaa tgaatagaaa agagaaacta gaatgtatat
    46021 tactattact cagtttaatt ctttcactaa tttctctatt gggaagtttg tatttttgat
    46081 atcaacaatt ttgtggattt gggttctatt tagagtaaat atcatttttg tttggtgatt
    46141 aaggagttta ttaaaattaa ttttccctac ttcaaatgca aggtaacaag atatagcact
    46201 tttaggagga atatttagcg gtacagcttc agagtatatt ctattatcat cgactgatcc
    46261 tcctttagca attaatttct taaatggagt agctgaccat ctgcggttta agttagcgga
    46321 tgaaagttct aaattaacta acatagctgg ttctgttgaa tagttagaaa tgattacttt
    46381 tgtataaatc atatctgcag cgaagtaaga agcattaagt tcaacttcta gttgagggcg
    46441 cattcctttt atttttatcc ctgttaaaaa cgtactcaat ataaaactta ttattgacat
    46501 ccattgaaaa attgttaagt gaaaaaaatt caaaataaac accaccagtt tttaactaaa
    46561 ttataccaaa aaggagagaa gaaataatgc aagaattagt aattttgaaa aataaagaag
    46621 ctgtgactac gagcttacaa gtggcagaaa gcttcgagaa aaaacatcaa catgttttaa
    46681 gagatattga tgcactaaaa aaagatgtgt ccaattttgg acagatgttt gtagaaggta
    46741 atgaaccaga ttcatatggc agaaatcgac gagttttctt cattagtaga gatggatttt
    46801 tcttgctggc tatggggttt acaggaaaga aagctatcta cttcaaacaa aaatacattg
    46861 aagcattcaa cgaaatggaa gatgttattc gcaagaatac tgttcctcaa acaattgaag
    46921 atatgatgat ctatcaatta gaagaaatga aagatgttaa aaaagatgtt tccatgctta
    46981 aagatactat gcgaattagc ggacaacaag agtttgaaat taagcaaaaa ggaaatatga
    47041 aagttatggg agttctaggg ggaaaagaaa gccgagctta tgaagaaatc agcaaaaaag
    47101 tattctcaaa attttggtct gaatttaaac gtaccttttc aatcccaaga tatggcgagt
    47161 tacctcgtaa gagattcgat gatgctgttt catttattga aatgtggttg ccagaaactg
    47221 cgatccgtat ggaaattgat caactgaaca gacaacagag actttttggt gatgacaatg
    47281 aatagagctg aagcgctaag aatagggacg gcaattgcta attgctggtg gaaatactat
    47341 aaaccaatca tcctaagcca acaacatatt gacaagcaaa aagcatggca acaaataaaa
    47401 aagtgactcc gccggcaagc aaagagtcac aaagaaaaca catcataagg agattttagc
    47461 atatggaaaa agaactttcc actctagatc aatatttgat tgatcctgat tggggcaagc
    47521 cgaaaattga ggaaacaagt ggtcgaaaaa tcagacgaaa tcttttgacg aatgaagaac
    47581 tagcttggga tcaagatgat ttaggcaacc atgtaactat ttgggatcat gtttatctta
    47641 tccatctatc gaagcattcg aataaacctg aatatattta cgtcatcgaa gatggcttga
    47701 ttgatgcgct agaagagtac gacagagata acttgattga tatctcttat tacggaccag
    47761 gtaagaaata cattgctgaa atggaggcag aatttgatga gtgaaatcaa agggacaacg
    47821 aactttgaaa aactttttag tcgtaagtta aataaaattc tcaagaaaaa aggaaatttt
    47881 gattatttat cttgggctca cgcgtgggag attatgaaaa agaatgatcc acaggcaacg
    47941 gtaactatta atgagtacaa acactacagg gttgtttctg gaactcatca agactttctt
    48001 gttgaggaat ataaaccttt tcttatggat gaaactggga cttatgtatc tgtctcagta
    48061 acggttaaag gacacacgga aaccgaatta tttcctgttt tagattatcg aaaccaacca
    48121 gttgttaaac caaatgcaat gcaaatcaat aactcattga agcgatgctt tgtgaaagca
    48181 ttggctctac acggactggg attatatgta tttcaagggg aagatattcc aacaccacct
    48241 agaatcgata caaagaaatt aaacatgcta gagacgattc tagaagcttt caatgagcag
    48301 atgggtaaag atatgaccaa aaccttaatc gaatatgtta atgagcagac agataaatta
    48361 gggctcttag ctgataacgt tgaaactatt gaacagttaa gctatgagca atgtgccttg
    48421 atggagcgag caatagcagc taagagaaaa gaattagata agaagtgata tgagtgttta
    48481 aaccattaat cgattcatat tcagcggttc tgaaaaagtt caaaggaaaa gacataagcg
    48541 caaccatcaa tgaggaagtg aacattgatc gactaaagac gatgtatgac ggctacgatg
    48601 gtgatcgagt cattgaaatt cgttttattg atcctagacg tttcaccgta cagcaacgaa
    48661 acttcatcta tgcgctgata ggcgatattt ttatcgatac aggcatgcca acggacttct
    48721 ggaaggaatt cttctacttt cgttttgaag gtgtcacagg gcgcaaaata agcctgaaag
    48781 acgaatcgaa tacaactgtg agtgatgcca atgtcttagc aaatatcatc ttagatttca
    48841 tctttgaaca tcatattcct ttcaaagaag gctatgagat tttacctgcg aatcaagaat
    48901 attacttcta caaatgcatc acaaaaagag tctgctgcat ctgtggcaaa acaggagctg
    48961 acatcgatca ctttgacaaa gcgctaggaa gacgaaagcg caaagaagtt gatcatgcag
    49021 agtacacatt tgcagcactc tgcagaatcc atcacacaga gaagcacaaa ataggtgtga
    49081 ttaatttcaa aaataaatat caaatcaaag ggatcaaatt aaaccagaaa acaatcaaaa
    49141 agttaaatat tggagggtaa aaatgacaga acatcgaagt tattatgcga ttataccagc
    49201 caacgtaagg tacgacaaaa gacttaaacc aaatactaag ttgttatacg gagagataac
    49261 ggccttgtgt aatgaaagag gcttttgttg ggcaggcaat gagtactttg cagatttata
    49321 tggtgtgaat aaagagacca tatcgcgatg ggtaagtgat ttgattaagt ttggatactt
    49381 gaatcgggaa atcatttaca aagagggtac caatcaaata atcaataggt acctacgaat
    49441 taatcaatac cctattgacg aaaaacgcaa tacccctatt gacgaaaaag tcaaagataa
    49501 taatacatct attaataata catttaataa tacaaaagaa tatataagag agttaccgcc
    49561 ttcgaaaaaa tcgaaggcta agcccgtccg tcataaatac ggagagtata aaaatgttct
    49621 tttgtcagat gagcagatgg agaaactcaa aacagaattc cctaatgatt accaagagcg
    49681 aatcgaacga ctgtcagagt attgtgaatc atctggtaag acttataaaa actatttggc
    49741 aactattcga agttgggcaa ggaaagaaaa aagtgaacct aagaacgcaa gcagtggata
    49801 caagcgcaca ggaagacgag agaagcttcc tgaatgggca atcgaccaag aagcctatct
    49861 caagaaaaaa gcgctagaac gagctaatag acaatcaaaa gcaccattct aagaggtgga
    49921 aaaatgaaga tcgattatct agaactaatt aatgaaatag cgaattataa aaagggcgag
    49981 gaattagacg tcctgagaga cgtatatgat caactcgaag aagctggaat tgaaggaatt
    50041 aagaatgatc gttcgagttg gagtaagctc agatactatt tcgcacttta tatcgataca
    50101 acacaattaa gaaatttagc atataccaaa ttactatttg ttgattgtgt taaaggcttg
    50161 caaaaacatc ttaatgaact tgagcaggtg taatcagatg gacctaaaga catttacagc
    50221 acagattgaa ctaatgcatc aagaagcttt aagacaaagt gtatcgtacg aagacaagtg
    50281 gctcaacacg ttccatggcg gacgtgagag cgcacttgat caagtactca aattactgaa
    50341 aggagaatgt cgggatggat aagaaagcgg caatgcagcg aattatcgaa ttgacttatt
    50401 cagaagattg gcaaaatgac aaagaagctg cttcagaagt gatgaggctt ggaagagcga
    50461 tgtgggcaga caagagcaac aagccaagac cacgaaaaat cgcaatttgg cacggtgaca
    50521 aacttctagt gatagggaca gctgaacagt tagcaagtct cacaggcttg cacgagaaaa
    50581 tcgtgaggaa aagagcaagg tgtggctaca cagacgctaa gaagagaacg tttagatacg
    50641 tggaggaatc atcatgacaa cagaagaagt gattcaaatg cgtattcgaa gccttcagcg
    50701 tgagattgac gatctggaac gaacaaaggc agtgatggtc aatgaaacgg cgagaaaggc
    50761 aatcgatttg cacatagaga atttaagaag ggaaatccat cgattggagg aatgagcgtg
    50821 gataagaaag cagcaatgaa aagaattgct gaattaacca agtcagaatc ttggcaagaa
    50881 gacaaagaaa tagttgcaga agtccaaaag ctcggtaaat caatgtggac tgaaaagccc
    50941 aaacggagaa cgccgagaaa aattgcaatc tggcatgatg accgaatttt agtaacaggt
    51001 ac
//
LOCUS       DAFEPQ010000013        48438 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.013, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000013
VERSION     DAFEPQ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.013"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            97..540
                     /locus_tag="LP543_000854"
     CDS             97..540
                     /locus_tag="LP543_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL2321036.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(546..1730)
                     /locus_tag="LP543_000855"
     CDS             complement(546..1730)
                     /locus_tag="LP543_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HBL2321037.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            1998..2696
                     /locus_tag="LP543_000856"
     CDS             1998..2696
                     /locus_tag="LP543_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBL2321038.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2703..3380
                     /gene="nth"
                     /locus_tag="LP543_000857"
     CDS             2703..3380
                     /gene="nth"
                     /locus_tag="LP543_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBL2321039.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3506..4039)
                     /locus_tag="LP543_000858"
     CDS             complement(3506..4039)
                     /locus_tag="LP543_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321040.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4336..6717)
                     /locus_tag="LP543_000859"
     CDS             complement(4336..6717)
                     /locus_tag="LP543_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBL2321041.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6707..7354)
                     /gene="recU"
                     /locus_tag="LP543_000860"
     CDS             complement(6707..7354)
                     /gene="recU"
                     /locus_tag="LP543_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBL2321042.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7418..7957
                     /locus_tag="LP543_000861"
     CDS             7418..7957
                     /locus_tag="LP543_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HBL2321043.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8047..8466
                     /gene="gpsB"
                     /locus_tag="LP543_000862"
     CDS             8047..8466
                     /gene="gpsB"
                     /locus_tag="LP543_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBL2321044.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8488..8855
                     /gene="rnpB"
                     /locus_tag="LP543_000863"
     ncRNA           8488..8855
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="LP543_000863"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9064..10230
                     /locus_tag="LP543_000864"
     CDS             9064..10230
                     /locus_tag="LP543_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBL2321045.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10276..11772
                     /locus_tag="LP543_000865"
     CDS             10276..11772
                     /locus_tag="LP543_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBL2321046.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11853..12536
                     /locus_tag="LP543_000866"
     CDS             11853..12536
                     /locus_tag="LP543_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL2321047.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            13181..14371
                     /locus_tag="LP543_000867"
     CDS             13181..14371
                     /locus_tag="LP543_000867"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBL2321048.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            14473..15426
                     /locus_tag="LP543_000868"
     CDS             14473..15426
                     /locus_tag="LP543_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBL2321049.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            15495..16844
                     /locus_tag="LP543_000869"
     CDS             15495..16844
                     /locus_tag="LP543_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2321050.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(17027..17350)
                     /locus_tag="LP543_000870"
     CDS             complement(17027..17350)
                     /locus_tag="LP543_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321051.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            17479..18642
                     /locus_tag="LP543_000871"
     CDS             17479..18642
                     /locus_tag="LP543_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBL2321052.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            18712..20061
                     /locus_tag="LP543_000872"
     CDS             18712..20061
                     /locus_tag="LP543_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL2321053.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(20106..20537)
                     /locus_tag="LP543_000873"
     CDS             complement(20106..20537)
                     /locus_tag="LP543_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2321054.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            20925..23567
                     /gene="alaS"
                     /locus_tag="LP543_000874"
     CDS             20925..23567
                     /gene="alaS"
                     /locus_tag="LP543_000874"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBL2321055.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            23767..24363
                     /locus_tag="LP543_000875"
     CDS             23767..24363
                     /locus_tag="LP543_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL2321056.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            24485..25633
                     /locus_tag="LP543_000876"
     CDS             24485..25633
                     /locus_tag="LP543_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBL2321057.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(25671..26084)
                     /locus_tag="LP543_000877"
     CDS             complement(25671..26084)
                     /locus_tag="LP543_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBL2321058.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            26163..26594
                     /locus_tag="LP543_000878"
     CDS             26163..26594
                     /locus_tag="LP543_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBL2321059.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(26693..26926)
                     /locus_tag="LP543_000879"
     CDS             complement(26693..26926)
                     /locus_tag="LP543_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBL2321060.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            27109..28779
                     /locus_tag="LP543_000880"
     CDS             27109..28779
                     /locus_tag="LP543_000880"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBL2321061.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            28926..29312
                     /locus_tag="LP543_000881"
     CDS             28926..29312
                     /locus_tag="LP543_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321062.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            29299..29997
                     /locus_tag="LP543_000882"
     CDS             29299..29997
                     /locus_tag="LP543_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL2321063.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            30002..30466
                     /locus_tag="LP543_000883"
     CDS             30002..30466
                     /locus_tag="LP543_000883"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBL2321064.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            30463..31374
                     /locus_tag="LP543_000884"
     CDS             30463..31374
                     /locus_tag="LP543_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL2321065.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            31609..32148
                     /gene="pyrR"
                     /locus_tag="LP543_000885"
     CDS             31609..32148
                     /gene="pyrR"
                     /locus_tag="LP543_000885"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBL2321066.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            32162..33445
                     /locus_tag="LP543_000886"
     CDS             32162..33445
                     /locus_tag="LP543_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBL2321067.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            33458..34384
                     /locus_tag="LP543_000887"
     CDS             33458..34384
                     /locus_tag="LP543_000887"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBL2321068.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            34403..35683
                     /locus_tag="LP543_000888"
     CDS             34403..35683
                     /locus_tag="LP543_000888"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBL2321069.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            35688..36764
                     /gene="carA"
                     /locus_tag="LP543_000889"
     CDS             35688..36764
                     /gene="carA"
                     /locus_tag="LP543_000889"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBL2321070.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            36769..39954
                     /gene="carB"
                     /locus_tag="LP543_000890"
     CDS             36769..39954
                     /gene="carB"
                     /locus_tag="LP543_000890"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBL2321071.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            40008..40784
                     /locus_tag="LP543_000891"
     CDS             40008..40784
                     /locus_tag="LP543_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBL2321072.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            40781..41713
                     /locus_tag="LP543_000892"
     CDS             40781..41713
                     /locus_tag="LP543_000892"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBL2321073.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            41710..42426
                     /gene="pyrF"
                     /locus_tag="LP543_000893"
     CDS             41710..42426
                     /gene="pyrF"
                     /locus_tag="LP543_000893"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBL2321074.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            42433..43062
                     /locus_tag="LP543_000894"
     CDS             42433..43062
                     /locus_tag="LP543_000894"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBL2321075.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            43050..43364
                     /locus_tag="LP543_000895"
     CDS             43050..43364
                     /locus_tag="LP543_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321076.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            43402..44043
                     /locus_tag="LP543_000896"
     CDS             43402..44043
                     /locus_tag="LP543_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL2321077.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            44086..44778
                     /locus_tag="LP543_000897"
     CDS             44086..44778
                     /locus_tag="LP543_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBL2321078.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            44775..45677
                     /locus_tag="LP543_000898"
     CDS             44775..45677
                     /locus_tag="LP543_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL2321079.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(45835..47541)
                     /gene="efbA"
                     /locus_tag="LP543_000899"
     CDS             complement(45835..47541)
                     /gene="efbA"
                     /locus_tag="LP543_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HBL2321080.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            47731..48354
                     /locus_tag="LP543_000900"
     CDS             47731..48354
                     /locus_tag="LP543_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321081.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
BASE COUNT        15786 a         8462 c        10425 g        13765 t
ORIGIN      
        1 tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa taggaaacaa
       61 aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca actgctaata
      121 gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga aatggaaaaa
      181 gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg gaaaatccct
      241 gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc catcagtaag
      301 ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga agagaataaa
      361 aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag acacgagcaa
      421 ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc aaaagaagaa
      481 tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag tagcaaatga
      541 agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa tccgaatacc
      601 gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc gatgacgcgc
      661 gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt atctgagaca
      721 gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa accataacag
      781 ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt gatgaataga
      841 taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga aaattttttg
      901 agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat caaagagaag
      961 cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg agaaaagata
     1021 gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa aggaataacg
     1081 gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga gaaacttaaa
     1141 tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc ataaacggca
     1201 tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa ctcagctaat
     1261 agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc catccatcca
     1321 aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata taaggtggag
     1381 atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca tatcgaattt
     1441 cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat aaggatcgga
     1501 tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa ggcagctaga
     1561 gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga gtgagaataa
     1621 ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc agtgaataag
     1681 acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat gtaaaaactc
     1741 ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt gtttccttgt
     1801 caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag tgaagatacg
     1861 catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg accgatcagt
     1921 gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt atactgtgat
     1981 tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga caaacagtca
     2041 tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa gaatttttat
     2101 ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat ttgcagatga
     2161 ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat cgtctcgtaa
     2221 caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca gatcgatata
     2281 atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa aaaaaatacg
     2341 aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa caagaatttg
     2401 gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa gaagaccatt
     2461 atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag gcttatagtt
     2521 ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt acaaaacaac
     2581 aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa gtagaacagt
     2641 cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag gagtagcgga
     2701 atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga atgtttcccg
     2761 aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca gtgatcttaa
     2821 gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt gcctcttttc
     2881 cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa ataaaaacga
     2941 ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag ctgattgaac
     3001 gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct ggtgtaggca
     3061 gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc gcagtagata
     3121 cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct acggtcatgg
     3181 aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag acacatcata
     3241 cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc gaggtatgtc
     3301 ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa aaggcgctaa
     3361 aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta tgaattatct
     3421 tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt aaatgtacat
     3481 actcagtagc taggatggta acttattaca atttattgcg ataaaaataa aagttgttat
     3541 ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct ataaaataag
     3601 tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt gtaataaaaa
     3661 tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg gatttgggcg
     3721 gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa aaagtgagaa
     3781 tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt aaaagtacag
     3841 aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat aataaacaaa
     3901 atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa aaaagatggt
     3961 gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag ccttctttca
     4021 aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg ggattattaa
     4081 gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata aacaagtaaa
     4141 tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta agctaaatgt
     4201 tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac agcttggtac
     4261 atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac gcttgtagac
     4321 gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg gtgtatggtc
     4381 atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag gagcagatga
     4441 agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag acgctggctc
     4501 gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc tttgactagt
     4561 agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct ggacatagct
     4621 ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat attgatcttt
     4681 gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt ctgtatttgt
     4741 cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta cgtgtgacga
     4801 ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa tcgaataatt
     4861 tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac caagttttgc
     4921 atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata acccagcgat
     4981 ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg tgatcatata
     5041 agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt ctgttccgtc
     5101 ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat tggcgaaagc
     5161 cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat cttgttcttc
     5221 cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc caagattttt
     5281 taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg ctggaacatt
     5341 acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat tatcccagtt
     5401 tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag tttttccagt
     5461 agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca tcgtagaacc
     5521 aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt ctgcgatgtg
     5581 acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg tggaggcaac
     5641 ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat catatagttt
     5701 cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt agacattctt
     5761 atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga cgatcttagt
     5821 attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat taacagcttg
     5881 atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa cagtatctcg
     5941 tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg cttgaggcat
     6001 cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac caaagtacat
     6061 ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat ttacgtagta
     6121 ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc acgcttcttg
     6181 ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa gtttgatcaa
     6241 ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag agagggcaga
     6301 accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat cttctaccga
     6361 cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt cacgcttttc
     6421 tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag atacggtagc
     6481 gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga aaagtcctac
     6541 acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct tgataaataa
     6601 tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct tcttcacact
     6661 ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat ttgccattct
     6721 tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc aaataaggaa
     6781 tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa gataaaggta
     6841 gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga acactattca
     6901 aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc ccattttgtt
     6961 cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag ggaaaagatg
     7021 ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag acgccattat
     7081 agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca cttctcttag
     7141 gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc gccaacccgc
     7201 gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc ccccgattgc
     7261 cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag gcattcttat
     7321 aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct aggccacatt
     7381 atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa aagtaatcta
     7441 tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc ctaaaatcca
     7501 agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag ggctggaatg
     7561 ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag tagcggaatt
     7621 gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg attttggtaa
     7681 taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag cagattatgt
     7741 cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc acactcgttt
     7801 tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc ctggaaaaac
     7861 gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg aaattcggct
     7921 gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt tgatttatca
     7981 acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa aactagaggt
     8041 gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga attcaaaaca
     8101 aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat catcaaagat
     8161 tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg attgagtgca
     8221 aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca acaaacagag
     8281 gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc aaatcttgaa
     8341 cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc agcacaacct
     8401 aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa aacacgtcaa
     8461 ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga cttgatcagg
     8521 ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc ttagcgaaac
     8581 cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct tcggctatgc
     8641 ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat ccggtggaac
     8701 gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt gtctggaaga
     8761 agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg ttttttctcc
     8821 ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag ggaattaggt
     8881 cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta tttttcaata
     8941 cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta ttcggaattt
     9001 gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat aaggagattg
     9061 tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg gctagaagca
     9121 ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa cggacgtgca
     9181 cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg tacagcagat
     9241 cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt gtttgaaaac
     9301 gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc tgtagctgga
     9361 aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac aaaaaaagca
     9421 atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact gacggaaact
     9481 ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt aactctggat
     9541 acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg agcaccattg
     9601 aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga ccgtccattt
     9661 tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat aggccataat
     9721 atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt cagtcaagaa
     9781 atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat tgaattagat
     9841 attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa tgcagaagaa
     9901 atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt caaaacggat
     9961 aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg agaagaagaa
    10021 actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa aacatggagt
    10081 aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa agcaaccaaa
    10141 aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg gggaactcgt
    10201 ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc aattggatgt
    10261 ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga aatcaatctt
    10321 ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc tgaaaaagct
    10381 agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc aaaaaaaatc
    10441 ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga attgtcggaa
    10501 gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca tcgcgtaccg
    10561 gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca atggcaaaaa
    10621 gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa tattgaatta
    10681 acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga tgttttgctg
    10741 aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga tcagcttaaa
    10801 gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc tgaaacagac
    10861 tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa agtaatcgaa
    10921 caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc atttatgact
    10981 ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga tttcaaaatg
    11041 gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga catcgatgat
    11101 aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga atcacaatcg
    11161 ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca atatcctttc
    11221 tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt ttatcgttca
    11281 ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac ttaccctctc
    11341 catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt agatgttgct
    11401 gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg accaacaaat
    11461 gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg ttattttccc
    11521 tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca gcaagaaata
    11581 gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg gatgtcagag
    11641 catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga tgcaacacat
    11701 gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt taatgtatat
    11761 caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct atagatgttt
    11821 tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg atcgctgatt
    11881 atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat accaaaagag
    11941 ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc tttgtgacgc
    12001 ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat gagaacgagg
    12061 caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa cagatctcga
    12121 tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa gcggccgaac
    12181 tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta ggagcaatcg
    12241 gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt gatcctgaac
    12301 tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca gtgatcaatc
    12361 atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac ggatatgaag
    12421 aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt ctggaatggt
    12481 ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa aaatgatgga
    12541 ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta aaagttactt
    12601 ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt cacaaggaac
    12661 aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt cggaattggc
    12721 aggagaacac cgtttattcg gaagaatagg actgtgacaa gacttttgtc acagtccttt
    12781 tttcgaattc aacaagtaaa acttttaaga aatattcaga gagtaaaatt aaaaattgct
    12841 gataaacgaa gatggaaatt ttatgtattt ttattggtct atgcaataaa taaattaaat
    12901 attcagaata caattgattt taacgaaagg gcttctatca atggttgtgt tgtatttatc
    12961 tgcccaggta gagtaaaata gatcattatc taaaaggtaa ttgttcatac tatataagtc
    13021 atttgaaagt aaaaagatac tgtaaataaa aaatgtggtt aaataattct cataatcagt
    13081 gtaagcgtta tcaaaaatat agcatacttg ctttgtagat gaatgacacg atatatgttt
    13141 ggtatgttct gcaagacaga tgaatatgga ggtggagaca atgtcttcca aagaactaac
    13201 aatgtattta gagaaagaat tggatcaact gaaagaaaag gggttataca atacgatcga
    13261 catactagaa agtgaaaatg gtgcttgtat catcgtagac ggaaaaaaga tgatcaatct
    13321 agcatccaac aattatcttg gatttgccaa tcgcgaagaa ttgaaaaaag catgtattga
    13381 agcaaccgaa acttatggtg taggagcagg agcagtccga acgatcaatg gttcgctgaa
    13441 aatccatcag caattagaag aaaaaatcgc agaattcaaa gggacagaag cagcaatcgc
    13501 ctttcaatct ggattcaact gcaatatggg cgctatctct gcagtaatga caaaagaaga
    13561 tgctatctta tcggatgaat taaaccatgc atctattatt gacggttgcc gcttatccgg
    13621 agcaaaaatc atccgaatta aacatcaaga tatgaaagac cttgagaaaa aagcaaagga
    13681 agcgatagaa agtaagaaat ataaaaaaat catgtatatc acagatggtg ttttctccat
    13741 ggatggagac attgcacgat tgccggaaat tattccgatt gttgaaaaat atggactgat
    13801 cacctacgtc gatgatgcgc acggctcagg cgtaactgga aaaggtgcag gaaccgtcaa
    13861 gcattttgga ttaagcgaca aaatcgatat gcaaatgggc acgttatcga aagctatagg
    13921 tgttgttggg ggttatgtag ctggatcgaa aacactaatc gactggttga aagctagaag
    13981 tcggccattc ctattttcta cttcgctaac tccaggtgca gctgcagctg cattggcttc
    14041 gattactttg atgcaagaac atccggaact tgtcgaaaaa gtttgggaaa atgctaatta
    14101 ttttaaagaa gaattgaaaa aagtaggcta taacatcggt atgtctgaaa cacctattac
    14161 tccagtcatt ttgggagacg aaaaagtgac tcagactttc tctaagaagc tgatcgaaca
    14221 tgggatttat gctaaaccaa tcgtttatcc aactgtgcct cttgggacag gcaggatcag
    14281 aaatatgccg actgcggaac atacgaggga aatgcttgat gaagcagtag cagtttatca
    14341 aaaaatagga aaagaaatgg aaattattta actaaattta gttttttcgt ctatttaaaa
    14401 ttagagaaca tgttcacgca tatattgtta atcatctgaa gatagtagta aaaaagatca
    14461 gggaggatca tcatgaaaaa agtattggtg acaggatgtt taggacaaat tggttcagag
    14521 ttgatcacac ggctacgtca agaactaggc agtgaaaatg tcattgcgac agatatacgc
    14581 atgcctgaag tcattgagaa aaacgatatc tttgagatcc tagatgtaat ggattacgaa
    14641 aaaatgcgca gcatagcaga acaatatcaa gtggacacat tgattcattt ggctgctctt
    14701 ttgtcagctg ttgcagaagc aaaaccacaa ttagcctgga atttgaacgt gactgggctg
    14761 gtcaatgcat tggaagtcgc tagagaattc cagctgaaat tttttacgcc aagttcaatc
    14821 ggcgcgtttg gtccaaatac tccaaaagat cataccccac aagatactct tcagcgtcct
    14881 acaaccatgt atggggtaac gaaggtagct ggagaactac tatgtgatta ttattatgtg
    14941 aaatatggcg tagatactcg tggtgtccgt ttccctggat tgatttctta tgcaaccttg
    15001 ccaggaggcg gtacgacaga ctacgcagtc gatatttatt attcagcaat caaagaagga
    15061 aagtacacta gtttcattga tcaaggtaca tcaatggata tgatgtatat gccagatgcg
    15121 atcgatgcca ttataaaact catgaatgca gatcctgatc gattggttca tcgaaatgcc
    15181 ttcaatatat cggcgatgtc ttttgacccg gaagagatca aagcatctat tcaaaaaatc
    15241 ataccagatt ttgaaatgga ttatgatgtg gatcctgtac gtcagtcaat agcagaatct
    15301 tggccaaatt cactagatgt ttcttgtgcg caagaggaat ggggcttttc gccgaaatac
    15361 gacttagatg tgatgacaaa ggatatgttg gaaaaactga aagaaaaatt ggtagagacg
    15421 gtataaaaca agaaccacat ccaaaaagtt ttttatactt ttgggattgg ctatggtatg
    15481 aaaggggaca agggatggaa gaatatcaag ccacgccagt aaaagaagta aaaatagaaa
    15541 atgaattaaa acgtacgatg ggatttttta ctgctttatc gactgttatg ggcacagtga
    15601 tcggtgcagg ggtatttttt aaagccgcaa gcgtagcaga agttacagga tccgccagtt
    15661 tgcatatgtt ttcttggttt ctcggtggta tgatttctgt ctgtgcagga ctaacaggag
    15721 ctgaactggc agctgctatt ccagaaacag gagggatgat taaatatatc gaacggattt
    15781 acggaaatac agcagctttt ttattgggct gggcgcaagt cgtgatttat tttccagcaa
    15841 atgtagcagc cttgtcgatc attttcggta cacagttcgt caatctattt ggtctttccc
    15901 agtcaatgat tgttcccgta gctgttacag ctgctgtatc gatcctgttg atcaattttt
    15961 taggttctaa agctggaggg gcttttcagt cgattacgct tgtctgcaag cttattcctt
    16021 tgtttgtgat cgtcattttc ggtttgttcc gacaagaagg agtagacttc caactgtttc
    16081 cgatccaagc aggagaaaat ctttcatttt tctcagcctt gggagcgggc ttgctagcaa
    16141 cgatgttcgc atacgacggt tggatacacg taggaaatat ttcgggagaa ttgaaaaagc
    16201 cggcgaaaga tttgccaaaa gcaatttctt taggaatcat cggaatcatg atcgtttatt
    16261 tattagtaaa tgcggtgttc ttaagaaccg catctatcga cggagtcgca ggaaacagca
    16321 atgcggcaag tgacgttgca aaaatgattt ttggcggctt cggtggaaga ctggtcacag
    16381 ttggtatctt gatttctgtt tatggaacca tcaatggcta tacccttact ggaatgcgtc
    16441 tcccttatgt tatggcaaaa gaaaacaacc tgccttttag caagctcttt gctaaacttc
    16501 atgataaaac taaagttcct gtagccgcag gtatcttaga gctagtcatc gctattggca
    16561 tgatgatgat cggtggtttt gatacactaa cggatatgct gatctttgtc atttggattt
    16621 tttatacgat ggtctttgtt ggtgtcattc ttcttcgtaa aaaagaacct gatttgtttc
    16681 gaccatacaa agttccaatg tatccgttta ttccactagt agcaatcatt ggagggactt
    16741 ttatcgtatc aagcacgctt atcactcaga cctttttagc ttcgatgggg atagtattga
    16801 cacttgctgg tatcccgatt tatctctatt taaaacgtag atagctaaaa cattcgaatt
    16861 ttattagaac tgcacttctc tgccaaacaa aatattttat ttaaatgagg agtacgtgga
    16921 agaaatgtca aaagtactcg ttaattttga tcacgtaaat gtcgttttgt ctacgatgaa
    16981 atatcaaagt aaaagacaga aatcgggctc aaccgatttc tgtcttttac tttgatttgc
    17041 gtaactgaac tattcggcgt acgccgcgat aaatatttaa taaaaccaag caaccagaaa
    17101 tgaaaatggg aagccaaatc agatagacag ggtagccaag cactttagac aaagcatgtg
    17161 gagtcatttt atccatttca agaagtgctt gctgccaagt ctgaagattg aagactagca
    17221 tgaaacccga aataatcagg agaataaatc ctaaataatg aattgtagtt ctttttctta
    17281 cataagcgat cgtagcataa aggaacagta aaaaaatagg tacgccaaaa acaaagtaca
    17341 tattcagcat ctcgacaccc cctttgtgtt tatcttactt tactttgaaa gaaatgagaa
    17401 ataatagcct cgttacttgc ttgaaacagc aaaggactgc taagataaga gagatacaaa
    17461 gaatcaagga ggagtcatat ggcaagttat atcgaaccga ttcgttttgc gctgattgtt
    17521 tttccatttt tagccttagc gatctcatct gtttttttta tttatgaata tcgaaagtat
    17581 ggaacatttc tagtaacaag ggcagttatt ttgtattctt tcgtttttta tttattgtgt
    17641 gcgtattttt tggtgatctt accacttccg gcaaaggaga cggttgccca aatgacaggg
    17701 ccaaagatgg agctgcattt atttgcttca tggcaacatt tcagagacaa cacggtattg
    17761 gtcttgtctg aacctagtac ctacctgccg gcaatgaagc agagtgtatt tttggaacca
    17821 gcttttaatg ttttattact cataccattt ggcatttatt taagatatta tttccggctt
    17881 tcttttatga aaacgatgat tgctagtttc tgcctttctc tattttttga attgacgcag
    17941 ctaagtggac tttattttat ctacccaaga ccataccgct tatttgatgt gaatgattta
    18001 gtgacaaata cattgggtgg aatcattggt tttgtcgtta cgccgttatt tacatttatg
    18061 cttcctacaa gaagacgaat ggatgaactt tcttatgaaa aaggaaagac tgtctcattg
    18121 actcgacgct tggtcgctta tttgatcgac tggacatttt tatctcttat taatttggta
    18181 gcggcgattt tctttaaaat tgcgttcgat tcaggggcag ttacttctca tatctggtgg
    18241 cttttattag aagtaggggt atactttatg attgtaccgt ttataaccaa tggatggaca
    18301 cttggcaaaa aaattgtgcg tatccgtttg gtggaagaag accgcgaacg aatttcttta
    18361 aaagcattga tgatccgcta cgggtatctg tactttcttt tttatggttt aagctggttg
    18421 gggcagttgc ttcagcaaat catgaattcg acaacgaata cggtgttgca gatgagtgca
    18481 gtattcattt ctctcggtgt cttgttacta caaggtattt ttattttaaa tatcttgcta
    18541 tcatttttga gaaaaaaacg gattctgttt tatgaaaaag ctagtcacac atatactgtt
    18601 agcacgattg gccaaacaaa ggatgatgat tctcgaaatt agctgagcaa aaacgctttg
    18661 tctgtgttat aatgccaaag tcgtgataat ttaaagagaa gaggtgcacg aatgaataat
    18721 tttgatatat tccgcttcaa accgtttatc aatgaagcgt taaccgcaaa gggcttcaaa
    18781 cagcccacag aggtccagga acgtttgatt cctttaattg caaaaggaaa aaatatcatt
    18841 gggcaatctc aaactggtac aggaaaaaca catacgtttt tgttgccttt gtttgaaaaa
    18901 atcaatgcag aaaaagagga agtacaagtt gttattacag ctccaagtag ggaactagct
    18961 gcacagatct atgaagcagc tacacaaatc gctaaatttt ctacaccgga gatccgtgtc
    19021 tcgaattttg tcggcggtac agacaaacag cgtcaattaa atcgtctgca tcaccaacag
    19081 ccacaggttg tagtaggcac gcctggtaga atattagaca tgatgaatga acaagcgctg
    19141 aagattcaca ccgcatttgc atttgttgtc gatgaagcgg atatgacatt ggatatgggt
    19201 tttttaaatg aagtagacca aattgcaagt cgattaccag aaaagctgca gtttttagtt
    19261 ttttctgcca ctattccaga aaaacttcgt ccattcttac gaaaatacat ggaaaatcca
    19321 atcattgaag aaatcaaacc aaaagcagtt atttctgaag acattgaaaa ttggctgatt
    19381 tcaacaaaag gtaaagatga gaatcaaatg atttatcaac tgctgacaat tggtcatcct
    19441 tatttagcaa tcgtatttgc caatacaaaa cagcatgtgg atgaaattgc cgattacttg
    19501 aaaaaccaag gcttgaaagt agcaaagatt catggagaca tcaccccaag agagagaaaa
    19561 cgagtgatgc gacaagtaca gaatctggag tatcaatatg ttgtagcgac tgatttagct
    19621 gctagaggga tcgatatcga aggtgtctct catgttatta atgcagaaat tccagaagat
    19681 ctagacttct ttatccatcg tgtcggacgt actggacgta atggcttaaa aggaacggcc
    19741 attacgttat attcgccaaa tgatgagcaa gccattgatg cgatagaaaa attgggcgtc
    19801 tcatttgaac caaaagaaat caaaaacggg gaaatcgtca aaacatacga tcgtaataga
    19861 cgtgccaaac gagaaaaatc aaaagatgta ttagatccca cgctgattgg tcttgtgaaa
    19921 aagaaaaaga aaaagatcaa gcctggttat aagaaaaaaa tcgattgggc aattgcagaa
    19981 aaaaataaaa aagaacgcaa aatcgaacgc agacagcaaa gccgtactgc tcgtaaaaat
    20041 aagaaaaatt cacataaata aaaaacaaga ggttgtgaca aaaggcgtgt cacaatctct
    20101 tgtttttaag ctagactttt tttcattccg atacaaagta cttcatcttc aataaaaggt
    20161 tctgtgcaag tatgataccc ttgtttttca taaaaatcta cagccgtcat ttcagctact
    20221 acataactag tctgacaacc ttctgaaacc gcccgttttt cagcagcttc aagcaactga
    20281 ccgcctaacc cttttcctcg gtattctttt gctacgcaaa atcgttccgg cttgaagcaa
    20341 gtaggtgttt tcttttcata acgcaaagta gctactggtc ttttttgatc aagaaataga
    20401 taataggttt gttgcgggat atcttttccg tcgaattcta gttctgcagg aatcccttgt
    20461 tctaatacaa aaacttgata tctcaaaaag cagctagctg cgcgtatcca ttcatccgct
    20521 ccaactattt ttttcatcat attcctcctt gtcatgaata gcagagatgt attgattcct
    20581 ttattcttat cgtaccaaaa atcgtggagt atgaccaaaa ggaaatgaaa ataaaaattg
    20641 agatattgac agtgatcaat agaatgcgta tactaaataa gtacgaaaag gatatgattt
    20701 gttttttaag actcacagaa aattctttct tggctgaaaa aagaatagtc actaatcaaa
    20761 ttgctccctt tttctatttt ggtagaaata ccgacgcagc tcagcgttat cgagtttaag
    20821 aggtaatgat tacacatcat tgcaatcaag gtggtaccgc gagttttcgt ccttggctgt
    20881 gatggtgttt ttttgtattt aatttataga aagcaggtaa tgacatgaaa caattaagta
    20941 gtgcacaagt tcgacaaatg tttcttgatt tcttccaatc taaaggacat acgattgaac
    21001 ctagtgcgtc tttgatcccc gtgaacgatc caaccttatt gtggatcaac tctggtgttg
    21061 caacattgaa aaaatatttt gatggttcag ttgtgccaga aaatccaaga atcacaaatg
    21121 cccaaaaatc tattcgaaca aatgatatcg aaaatgtcgg taaaacagca cggcaccata
    21181 cgatgtttga gatgctgggt aatttttcga ttggtgacta tttcaaaaat gaagccatcc
    21241 actgggcatg ggaatttttg actgatccaa aatggatggc attcgatcca gaaaaacttt
    21301 atgtaacagt ttatccaaaa gatacagaag caaaacgcat ctggcatgaa gaagttggcc
    21361 tgccactaga tcacattatt gatgtagaag acaacttctg ggatatcggt gcaggcccat
    21421 gtggtccgga tacggaaatt ttctatgacc gtggagaagc ttataacgat ttagcagaag
    21481 acgatcctga aaactatcca ggaggagaaa atgaacgtta tttagaaatc tggaacttag
    21541 tattttcaga attcaaccat aagcctgata acacatttga accacttcct cataaaaaca
    21601 ttgatacggg gatgggattg gaacgcatgg tctcaatcgt tcaagatgcg tcaacgaact
    21661 ttgagacaga cttgtttatg cctattatcc acgccgtaga aaagctaagc aaacaagtaa
    21721 aatatggtga aaacccagta acagatattt cttacaaagt aatcgcagat catattcgcg
    21781 ccctttcatt tgctattggc gacggtgcat tgccatcgaa tgaagggaga gggtatgtct
    21841 tacgccgttt gcttcgtcgt gctgtcatgc atgggaaaaa attaggtatt gaacaagcat
    21901 tcttatatcg tttagtccct gttgttgggg aaatcatggt aagttattat cctgaagtac
    21961 ttcaacaaaa agaatttatt gaaaaagtta tccgtacaga agaagaacgt ttcaatgaaa
    22021 caatcaatga aggtttggaa atcttaaatc aagtgatcca agaagtaaaa aacgaaaacg
    22081 ggacgactct agcaggaaaa gacatcttca aactatacga cacatatggc ttcccggtag
    22141 aattaacgga agaggtggca gaagatgaag gactgaaagt tgatcatgct ggttttgaaa
    22201 aagaaatgga tgcacaacgc gaacgtgctc gctctgcccg cagtactgaa aaatcaatgg
    22261 gggtacaatc tgctttattg acagatatca aagtagaaag taaattcatt ggatacacag
    22321 aacaagaatc agatagtcaa ttattggtca tcgttaaagg tgagtctctt ttgaatgaag
    22381 tcgcagaagg tgaagctcaa atgatctttg atcaaacacc tttctatgca gaaatgggtg
    22441 ggcaagtagc ggatcaagga tggatcatga atgaagtagg cgaaatcgta gcagttgtta
    22501 cagatgttca aaaagcgcct aatggtcaat ttatgcacac cgtagaagta aaagcaccaa
    22561 tgagtgaagg acaaacctat catctattga tcgatcaatc gatgcgcaat cggatcatca
    22621 aaaaccatac agcaacccat ctactgcaca aggcattgaa agaagtactt ggcgagcatg
    22681 caaatcaagc tggttcttta gtcgctcctg gacatttacg attcgacttt acccactttg
    22741 gtcaaatcac ttcagatgaa ttagctgaaa tggaaagaat cgtcaacgaa aaaatctggg
    22801 aagctatccc agttgaaaca attgaaacag atatcgatac agccaaaaac atgggtgcaa
    22861 tggctttgtt tggtgaaaaa tacggacatg acgttcgagt agtaaatatt ggcggctggt
    22921 caatcgaact gtgcggaggc acacatgtag caaatacaga aaacatcgga atcttcaaaa
    22981 tcgtttctga atcaggaatt ggtgcaggcg tgcgacgaat cgaggctgta acaagtaaag
    23041 aagcgtacga actacttgaa gaagaagaaa aacaactaaa acaagtagca acaattgtta
    23101 aatcacctca attgaaagaa gttgtttcaa aagcggaaca attgcaacag caagtaagag
    23161 agctacaaaa ggaaaatgaa caattagcta gcaagttagc gaatcaacaa gctggtgatg
    23221 tattcaaaga tccagaaacg atcaatggaa aaacaatcat tgcagcacaa gtaaaagtga
    23281 aagatatgaa tcagctgcgt caattagctg accaatggaa acaaaaaaat atttcagata
    23341 ttcttgtttt agcaaacgaa caagaaggaa aagtcagtct gctagcggca atgacgaaag
    23401 aatcaaatga agctggacta aaagctggcg acttgattaa agcaatcgca ccgaaagtag
    23461 gcggtggcgg tggtggacgt ccagacatgg cacaagctgg cgggaaaaat cctgctggta
    23521 ttccagatgc acttaaagct gcgaaagaat gggttgcgca acaatagggc gtttacgttt
    23581 gacagaaaaa caaaagttca aacattgaac gaaacatgat tatgggggcg aagacaaaca
    23641 agtcttgccc tcatactttt ttcaattgga tggcgcatct aatcaaatgg tcggattcgg
    23701 atggtcttta gttgacgaaa gtgacagaaa tttttacgat tagtataaat aaaaaggggt
    23761 gaaaaaatga atagtagaaa taagaccttt cgactagtct tacgtgcgat cttgttagca
    23821 atcatcatcg tacaagcgat ggttccttgg ctggggttta ttccattagg attcattagt
    23881 ttaacaatta tccatatcac agtaattatt gctgcagtag tattaggccc aaaagatggc
    23941 atggtgattg gcttgttttg gggaatcgct acaattgttc gtgcctatgc catgccaaca
    24001 acgccatttg atacattagt gttcacgaat ccgattatct cggtagttcc cagagtactt
    24061 gttggcttag tagcaggctt ggttttccac tggatttatc aaaaaaataa atcaataacg
    24121 atcagctcag cctttgcagg ggtactgggt tcattggtca atacagtctt agtgttagga
    24181 ttcatgggat tattttatac aggtgcaacg gcagatgcct atggtgtaga ctctgctctt
    24241 ttatttaaaa ccttagcagg ggttgcagca atcaatggga taccagaagc aataggtgcg
    24301 ggaatcataa caccattact agcaaaagcg ttatttgcag caacaccgtt gaaaccagaa
    24361 taatgaccat cttttaatag taataggacg cacgatagat agaaaaagga agagaggaga
    24421 ggaaaacaat cgtcctctcc atctttttac ctgagcaaca agcaaactag aaggggtaaa
    24481 aaacttggct aagaaacaag aagaaaaaaa gaaacaaata ccgcaacagc ggacaaaaaa
    24541 aggagtacta gcgggtatct taggattggt tacgaatctg atcttgtttg tctctaagct
    24601 aatgatcggg cttttttcag gaagtgtctc cattatggct gatgcagtga acagtctatc
    24661 agatactgct tcatccatat tgactttgat cggatttaaa attgctgcaa agccagcaga
    24721 tcaggaacat ccttatggac acgaacgctt cgaatatatc agtgggttat ttgtttcgat
    24781 catcattact tatgtaggat ttcaattttt agagtcttct gctgaaaaaa tcatccatcc
    24841 agaaaatgtc tctttaacac ccatcgtctt ttttgtccta atcatttcca tctttttgaa
    24901 atttgcacaa ggcaaaatgt atcaggttat tgcgcaatcc attgattctg aaacattgag
    24961 agctacagca acagatagtt ataatgatgt ctttactaca ctggctgtat tgctttcagc
    25021 agcaatcgaa tgggtaactg gatggcgtat tgatggatat ataggttttg ctttagcatt
    25081 atatatcctt tatagtggat tgacgatgct tcgaaatttt gtatacgaac tattaggaag
    25141 tcgcccaacg ccagcagaga ttgaagaaat ggaagagcat ttaaatgctt gcgatgatat
    25201 acttggctat catgatttat tggtgcataa ctatggaccc aataaacgct ttgcatcagt
    25261 ccacatcgaa gtagatgaat caatgaattt gaatgtggct cataagatta ttgatctgat
    25321 agaaaaagac tttaaaaagt ctttggatgt ggaacttgtc tgtcacttgg atccaatgcc
    25381 tgttgataac gaaaaatact ataatatctt gcggcagtta aagcaaattc ttcttgaaac
    25441 aggtacaggg cttaatattc atgattttag agtaattcat caaggaaaaa cgctgcaatt
    25501 tgatgtagtg gtccctgaga aattccagtt tgttgataca gaactagaaa gaatcttacg
    25561 tagtcaaata gaagaaaaaa tcggagatta ccaattggaa attacgtttg atcacaatta
    25621 tcttttatat taaatgagaa actataaaaa gactccttaa agtttttaat ttaaagagtc
    25681 tttttttctt ttaattgttt atggagagcc tgacgtgcaa gattatctgc tcctttattt
    25741 ttgttttcgg gcaaccattg caggatcaat aaagaaaatt cggatagaag ttcttggatt
    25801 ctttgaaggt agggaaggaa gagctcgttc ttcgttgcgt ttttgtcgac cgtagaaact
    25861 agtgttttgc tgtctgaaaa acagaaaatc gtttcattct gtaagtcgtt tttctttaga
    25921 aattctaaag tttttaaaaa gacttcgaac tctgcttgat ggtttgtccc tatagaaagc
    25981 gggatagata gctgttcttg tttaccttga tagacaatca agattccacc tccactagga
    26041 ccagggttgc cttttgtaga ggcatcaatg tatactttta acataaaaag agattctcct
    26101 ttttgttcaa actaatgtta tggtaagatt atatcattac tgaatcggtg agaggttaga
    26161 gaatgaaaaa aaatattcga tggcagcctg aactggcaca tacgatcatc tattggtcat
    26221 tgaccttgat cattctgttt tttagtctga ttctttcttt agaaaacaca ggtccttatt
    26281 ggaaaagcaa ttttgtttta ggtatttttc ttgtttttct atttctaggt ttgcggcgtt
    26341 cttttatttt aaaatcaaac tctgtgatta tacgttatgc tgctttttgg aaagaccgag
    26401 aaatatctgt cggacagatt cgatctttga cgccaactaa tactggggtg caaatgatgt
    26461 tgcaagatga gaaagcccca tctgttttca tgatgcgaaa aaaagatcaa gtagtccttt
    26521 ttgatcattt tgaaaaaaac ggacccaaat cagaatcaat tccttatcct tatacgaaaa
    26581 aaagaaaaag ctaattttaa aaatcagaac ctaaaaaaac gaatttttac aggaaaagaa
    26641 aagagtgaga cgtacggttc atcccgttta gcgtctcact ctatttgttt atctattcat
    26701 gcatactagt atcggtagtc tgaacgttgc ctttcttgac atgggcagct tgtgttcctc
    26761 gattcccttc gatgatctca aattcgacca cttgattttc ttccaaagtt ttgaatccct
    26821 cttcttcaat cgcagtaaaa tgtacaaaaa tttcttctgt gtcatcataa ctgataaagc
    26881 cgtatccttt tttgttgtca aaccatttta caattcctgt ggtcaaagca ctcacgacct
    26941 ttctaaagtc tgatcatagc cttcattata aagagtattt ataagtacgt caaataaatt
    27001 aaaaacctgt aattttatgt tttttttctg aataaaaaaa ggtataattg gcatatctta
    27061 acaaagaaga tattttgttg aataaagttc gtgaaaaggg gtaatcaaat gaagaatgat
    27121 ctgcaaattt cacaagaaac agagctagaa ccgattgttg aagtggctag taaattagat
    27181 cttaaggaag acgatttgga actgtatggg aaatataagg cgaaaatcaa tttttctgga
    27241 atcaatcgat caaaggataa cgctgagggg catttgatct tagttacttc catcaatcca
    27301 acaccagctg gcgaaggaaa gtcaacgatc actgtagggt taggagacgc gctcaataaa
    27361 atcaataaaa aatctgttat tgcattgcga gaaccctcac ttggtcctgt gatgggaatc
    27421 aaaggtggtg ctgcaggcgg aggatatgca caggtattgc ctatggaaga tatcaacttg
    27481 cattttactg gtgacatgca tgctatcaca acagctaata atgcattatc tgctctttta
    27541 gataatcata ttcatcaagg aaatgaattg aacatcgatg cccgccgagt gatttggaaa
    27601 cgggtagtcg atctcaatga tcgtgaactg cgtaaagtaa ttgttggctt gggtgggcca
    27661 atccaaggtg ttccaagaga agatgggttt gatatcacag ttgccagcga aatcatggct
    27721 atcttgtgtc ttgcggcaga ccttgaagat ttaaaggctc gtcttgcaag gatcgttgtc
    27781 ggctatacat ttgaccgtca gcctgtgact gctggagact tgaaagcaga aggcgctctg
    27841 gcattactgc tgaaagatgc aatcaagccg aatcttgtac aaacaattta cggaacacca
    27901 gcttttgtcc acgggggacc gtttgccaat atcgctcatg gctgcaatag tattttagct
    27961 accaagacag cattgagatt agcagattac gttgtcacag aggcaggatt tggtgcagat
    28021 cttggcggag agaaattttt agatatcaaa gtacctaact tgaaaaaagc gccggatgct
    28081 gtggtgattg tcgccactgt ccgtgcattg aaaatgcatg gcggaatgaa aaaagatgaa
    28141 cttaaaaatg agaatcttga tgctttgaaa gtaggttttg caaatttaaa gcgccatata
    28201 cgtaatatgg aacaatatca gctacctgtg atcgtcgcaa tcaatgaatt cgtaacagat
    28261 acagacaatg aactcacact gttggagcac ttgtgtgaaa atcaggggat tcttgccaag
    28321 agagcgagtg tatgggcaga tggagcagaa ggcggcgttg atttagcaaa agctgtagta
    28381 cgattgattg atagaaaaga agctaattac aagccattat accgcctaga agaaacgata
    28441 caagaaaaga cagaaacaat cgtcaagaaa atttacggag gaaatggtgt tgttttcagc
    28501 aaaaaagcgc aaaaacaaat agaagagttt acgaagaatg gatgggacaa actgccgatt
    28561 tgtatggcaa aaacacagta ttcattttct gatgatcctt cactgctagg tgcaccggaa
    28621 gatttcacca tcacgatccg cgaaatcatc ccaaaattag gggcaggatt tttagtagcc
    28681 ttgactggag atgttatgac gatgcctggt ttgcctaaaa aacctgctgc attgaatatg
    28741 gacgtgacaa atgacggaga agtgttggga ttgttttaag cttgattata atctaccatc
    28801 gaaggagctg tattttgact acagcttctt tttttagacg atagggaatt aattttgttt
    28861 acactaggat cattcttttt ctttattgta ttttgagcta tactaaagca taataggagg
    28921 gtatgatgaa aacgacattt ataagaaaaa ccggttttta cggtatgggt agctcgattc
    28981 agctaagaat caacaatgaa aaacgcagtg ttctcagtca tgaacaaaag ataacgcttg
    29041 atctggaagt gccctttacg atgcaggtca ccttttattg gctaaaaagc cccgtttata
    29101 cagtgaaaga agcaaagcct gcttatgtga tcacaatgaa ttacttgatt cttcaaatct
    29161 atccgttttt atttctagca actgggttgg gggccgtttt cctacaaaat ttatttttca
    29221 gtttttttgc agttattgtg ttgattgcgt tctttttcta tataaaaaac cgggcatatt
    29281 tgatcaagga ggaaaattat ggcaaactct gatctttttt taatcagttt caatcgaatc
    29341 gaaaaatgga tgagagacga gatgggaaat gcacgaaata tgggatttac cgaacttgtc
    29401 agacgcctag ctcaaagaaa acaattaatg atcagaaaat acgaagatga tcttttacag
    29461 cttgctcagt taaggaatgc gatcgtccat gatcggatcg cagtcgattt tgtcattgca
    29521 gagccaaatg attggacagt caagcgaatc cagctgatcg aacaagaact gattcagcct
    29581 gaaaccgtgc tgcctcgatt tgcaaaacgt gtaacaggtt ttgaacaaga tcttccgtta
    29641 cttgaactgt taaagatagt agcagaaaaa aggtattcac aatttccgtt atacaataaa
    29701 ggaaaatttg tagcattgat tactttacgt aatatcgggt tttggctagc aaaagaaagc
    29761 caaaaaggtc cggttgattt aacaaataaa cgagcgttgg atttgatcat ccaaaacgga
    29821 aagtacacaa attatcattt tgttccagct aaaacacata tttacgaggt tgaagcgatg
    29881 tttcgtgagc aagggacttt ggaagccgta cttatcacta aagatgggaa tcccgatggt
    29941 aatctgctcg ggatcgtacg acctcgtgat atttataaac aaatagaaaa ggattgattt
    30001 tttgttaatt atttattgga tattcagcgc tttgctagta ggagtggatc agttggtcaa
    30061 atggtggatc actgataatc tagcgcttgg agagacgaaa aatcttatac caggtatttt
    30121 gtcactgaat catattcgaa acactggagc cgcatggagt atgctagaag ggaagatgtg
    30181 gttctttact atagtgacac tcattgcagt cgttgtgatt ctcacattga tgattaaaaa
    30241 tcgtgaaaaa ggtaatcgct ggttcatgat tggattgagt ttgattctag ctggtgcgat
    30301 tggcaatttt atcgaccgta ttcgtcttgg ctatgtagta gatatgttcc agacagattt
    30361 tatgagcttt ccgattttta acgtagcaga tgtgacactt gtatgtggag tgatttgtat
    30421 gttaatttat attattttag atgaaaagga tcaaagaaaa aaatgacaga acttaaaacg
    30481 acgattcacc atgaatctgg taggatcgat aaagtattga ctaatttatt caccgattac
    30541 agtcgttcgc aaattcagct gtggctgaaa aatggagcgg tttctgtcaa tggacagcca
    30601 gtcaaagcaa attacaaagt aaaacaagga gacgagatta ctgtcttgat ccccgaacca
    30661 gaaacattag atgtggtccc agaaaatatc ccgttgacga tcgtttacga agatgaagat
    30721 gtagcggtga taaacaaacc tcaaggaatg gtcgttcatc cttcagcagg tcatacgcat
    30781 ggcacaatgg tcaatgcgct gatgtaccat atcaaggatc tttctacgat caacggcgtt
    30841 attcgtccag gtattgttca ccgaatcgac aaagatactt ccggactgct aatggttgcc
    30901 aaaaatgacc aggcacacga atcattagct cagcaattaa aagataagac ctctttgcgc
    30961 aaatatgttg ctttagtcca tggagagatt cctcacgaaa aaggaaccat caatgcgcca
    31021 atcggccgtt cgaaggaaaa ccggaaaatg caggcgataa gagaagatgg gaagtcggct
    31081 gtcacacatt ttactgtatt ggaacgtttc gaaggattta ctctggttga attaaaacta
    31141 gaaaccggaa gaacacacca gatccgtgtg catatgaagt atatcggtta tccgttagct
    31201 ggagatccag tatacggacc gaagaaaact ttgaaaggca atggtcagtt tctccatgcg
    31261 caaatgttag gttttacgca cccgactaca aacgaaaaga tgatctttga agcacctctt
    31321 ccagaagttt ttgaaaaaac tttagaaaaa ttaagaaaaa acattgcatt ttaatttaga
    31381 taacggtata gtaattaaca acgaaagcat aaaacttaat agaattcctt taagtttagt
    31441 cccgtgaggc taagaagggt gggagcaagg atacgtatgg tgcgccataa gtagattgct
    31501 cattgacata gtatagctat cagtgtatca gtatacgctc agtctaaaca ttcctcctgt
    31561 cctcttaggg caggagtttt tttatgcaaa aaagtagggg gaatgaaaat gcaagcaaaa
    31621 gaagtagtag atcaggtaac catgaagcga gcattgactc gaattactta tgagattatc
    31681 gagagaaacc atagtatcca agatatcgta ttggtaggta tcaaaacacg aggaatctac
    31741 attgcatcaa gaattgccga acgcctgaag cagctagaag atatcgatat tcctgtagga
    31801 gaactggata tcactttata ccgagatgac aaaaaagaaa atccagaaga accggaactt
    31861 cattcttctg acatcccagt ttccttagaa ggaaaagaag ttattttgat tgatgatgtt
    31921 ttgtatacag gacgaacgat tcgagcagca atggacgcag taatggattt tggcagacca
    31981 agaaaaattt cattagcagt cctggtcgat cgtgggcatc gagaattgcc aatccgtgca
    32041 gattatgttg ggaagaacat tccaacatct cgggcggaag agattttagt agagatgcaa
    32101 gagcttgatg gacaagatcg catcatgatt ttgaaagagg aagattagag gagagagaaa
    32161 catgtcggag aaaaaaggat ttcataatga tgacgtggtg ttagatatcc acgatcgccc
    32221 aaccccagga cattgggtag gattaagttt acaacacttg ttcacgatgt tcggagcaac
    32281 cgtgttagtc cccatcttag taggtatcga tccaggtatt gcgctagtca gttcaggact
    32341 aggtacattg gtgtacttat ttactactaa aggaaagata cctgcgtact taggaagcag
    32401 cttcgctttc attgcagcga tgcagctttt gatgaaaaca gatggttatc cagcgattgc
    32461 acaaggagca gtcacaactg gattagtcta tttgattgtt gcgctgatca tcaaaaagat
    32521 cggttcagca tggctggata aaatcttgcc tccaatcgtc gtcggtccag tcatcatggt
    32581 tatcgggttg ggacttgcgg caaatgcagc aacaaatgct atgtataatc agggcacgta
    32641 tgatttcaag tatattgctg tagcgctgat cacattagga ttaacaattt tcttcaacat
    32701 gtggctaaga ggttttctcg gattgattcc gattttacta ggtattatta gcggatacat
    32761 cgtagccttg ctgtttggaa tcgtggatac taagccaatc gtagacgctg cctggtttgc
    32821 tgttcctaac tttgagattc cctttgtaca atatgaacca aagctatatc ttggagcaat
    32881 cacaacaatg gcacccatcg cttttgtgac gatgacagag catatcggcc atcttatggt
    32941 attgaataaa ctaactaaac gtaatttctt tgaagaaccg ggtcttcata agacgttgac
    33001 aggtgatgga ttagcacaaa tcgtggcagc ctttgtcgga ggtcctcctg taacaagtta
    33061 cggtgaaaac attggtgttc tggctatcac acgtgtccat agcgtgtttg tgatcggtgg
    33121 agctgcagta tttgcagtca ttttaggatt cgtaggaaaa ttaagcgctt tgattttaag
    33181 tatccctgga ccagtcatct caggaatcag ttttatctta tttggagtaa ttgccgcaag
    33241 tggaatgaag attttaatcg agaacaaaat cgattttgat aaaaagaaaa atctgttgat
    33301 tgcttcagtt atcttagtag taggaattgg cggactcgtc tttgaagtag gcacatttac
    33361 attgtcggca atggcattag caactgtact tggcatcatt ttaaatctta tcttaccaga
    33421 aacttcacga agcgaagaaa actaaggagg aaacattatg atcatcaaat cagaacgtat
    33481 cagcttaaag catttattga ccgttgaggc acttactgat caagaagtta tgggcttgat
    33541 ccgcagaggg caggaattca agcaaggtgc taattggact ccgcaaaagc gccagtactt
    33601 tgctacaaac ctctttttcg aaaatagtac tcgcacacat aaaagcttcg atgtagcgga
    33661 gaaaaaacta gggctagaag tgattgaatt tgaagcaagc acaagctctg tccaaaaagg
    33721 ggaaacacta tacgatacag tactgacgat gtcagcatta ggcgtagacg tagcggttat
    33781 ccgtcatggt gatgagaact attatgacga actgatacaa agtaaaacaa tccagtgttc
    33841 gattatcaat ggaggagacg gaagcggaca gcatccaacc caatgcttgc ttgatttgat
    33901 gaccatttat gaagaatttg gtcattttga aggtctgaaa gttgccatcg taggtgatat
    33961 cactcactct cgcgtagcta aatccaatat gcagatgtta aaacgtctag gcgcacaagt
    34021 attcttctca ggtcctagag aatggtacga cgatgaattc gaagtgtacg gacattatat
    34081 gccattggat gaactactag atcaagttga tgtcatgatg atgcttcggg tgcaacacga
    34141 acgccatgat ggaaaagaaa gtttctcaaa agaaggctac catcaggaac atgggttaac
    34201 agttgagcgt gcgaagaagt tgcaaaaaca tgcaatcatt atgcacccag caccagttaa
    34261 ccgtgatgta gaattagctg attcattagt ggaaggatta cagtcgcgga tcgttcagca
    34321 aatgagcaat ggagtattcg tcagaatggc aatcttagaa gcagtgctaa atggaaaagc
    34381 gtaattatag gaggatgaaa aaatgaaaac actcattaaa aacggtcaga tcaatacacg
    34441 aaaaaatatg acgacaccag cagaaatttg gatagaagat gggcgaatca aagcaatcgg
    34501 tacaggattc tcagaagccg aatttgatga agtgtttgat gcaaagggac aattgatcac
    34561 acctggttta gtggacgtcc atgttcattt gagagagcca ggatttacat acaaggagac
    34621 gatcgaagct gggactcgct cagctgctcg tggcggtttc actactgtat gcgcgatgcc
    34681 gaatttaaat cctgtacctg atacggcaga aaagctgaga caagtatatg acatcattag
    34741 aaaagatgca gttgtgaagg tattgcagta tgcgccaatc acagaaaatc tgcgaagtga
    34801 gaaattagtt gatcaagaag cactgattga agaaggtgct tttgctttca caaatgacgg
    34861 tgtaggtgtc caaacagctg ggacgatgta tctggctatg aaagaagctg caaaaaacaa
    34921 taaagcccta gtagcccata cagaagatga atctttatta tttggcggcg ttatgcacgc
    34981 tggcaagaag gcagaagaac ttggtcttcc cgggatctta agcgtaaccg agtcttctca
    35041 aattgctaga gatctgttgc tagctgaagc aactggtgtt cattaccatg tttgccatgt
    35101 ttctactaaa gaaagtgtcc gtgtaatcag agacgcaaaa aaagcaggaa tccacgtaac
    35161 agcagaagtt tcaccccatc atcttatttt gatcgacgaa gatattcccg aagatttcgg
    35221 cttttggaaa atgaacccgc cgctaagagg acgggaggac agagaagctt tgatcgaagg
    35281 cttgcttgac ggtacgatcg attgcatcgc tacagaccat gccccccatg gattagaaga
    35341 aaaaagccaa agtttcatga aatcaccatt tggtatcgta ggaagcgaaa cagcgtttca
    35401 actgatctat actcattttg tagaaactgg tcgattcacg ttggaacaag tgatcaattg
    35461 gttggcagtt aaaccggctg agatttttgg tttgaatgca ggaacgctaa cggttggtgc
    35521 ccccgctgat gtagcagttt ttgatattac acaaacttgc acgatcgata aagaagactt
    35581 tttatcaaaa ggggagaata caccttttat cggctggaaa gtaaaaggcg aaacacaaat
    35641 gacttttgtc aacggaaaac ttgtgtggca gaagggagaa taaaagtatg gaacgtttgc
    35701 ttatattaga agatggaaca gttttcgaag gaaaggcttt tggtgcagat gctagtgtct
    35761 tcggagaaat cgtttttaca acaagtatga ctggttatca agaaactatc actgatcaaa
    35821 gttttaacgg tcaaatcatt acatttacct atcctatggt gggtaattat ggggtaaata
    35881 gagatgatta tgaatcgatt tctccaacat gcaaaggagt agtagtaaaa gaacatgcac
    35941 gtgtagcttc aaactggcga aaccaaatga cgctagattc atttttgaaa caaaaaggaa
    36001 ttcctggaat atcaggtatc gatacgcgcg cccttactcg caaaatccgt gaagtcggaa
    36061 cgatgaaagc tagtatagtt gatgctggag attccttcga acatgcattt gatcagctga
    36121 aagcaaccgt tctagcaacc aatcaagtgc agcaagtctc aactaacaaa ccttatccaa
    36181 gtccaggaac aggtcggaaa gtcattgtca ttgacttcgg cttgaaacat agtattttac
    36241 gagaattgag taaaagaaac tgtaacctca cagtacttcc ctataacaca gatgccgaga
    36301 cgattttagc gctatcacca gacggagtca tgctgacgaa tggacctggt gatccaaaag
    36361 atgtgccaga agcaatcgaa atgatcaaaa aaatccaagg aaaagtaccg atttttggta
    36421 tttgtctcgg tcaccagtta tttgctttag ccaacggtgc agatacctat aaaatgaaat
    36481 tcggacatcg aggattaaat cacccggtac gagaagtagc gaccggaaga attgatttta
    36541 cttctcaaaa tcatggctat gcagtcgatg aagccacgat tgatccagaa aaactcatcg
    36601 taactcatgt agaagtaaat gacggaacag tagaaggagt caaacacaga gattatcccg
    36661 ctttcagtgt gcagtttcat ccagatgctg cccctggtcc gcatgatgca ctacatttat
    36721 ttgatgaatt tatggaaatg atggacgcag gaaaggagca gtaaacagat gccgaaacgt
    36781 acagatatca agaaaattat ggtgattgga tctggtccga tcatcattgg tcaagccgca
    36841 gaatttgact acgcaggaac acaagcctgc cttgccttaa aagaagaagg atatgaagtg
    36901 gttttagtaa attctaatcc tgcgacgatc atgacggata aagaagtggc agatcaagta
    36961 tatatcgaac cgatcacact tgaatttgta tcaaggattc ttcgaaaaga gcgtccagat
    37021 gctctccttc caactttagg cggacagaca ggcctcaata tggcaatgga gctggcagca
    37081 tcaggtattt tagaagaatt gaacatagaa cttctaggaa caaagctttc tgcgatcgat
    37141 caagcagaag atcgtgatct attcaaacag ttgatgaaag aattgaatca gccaatcccc
    37201 gagtctgaaa tcgtcaatac agtagaagaa gctgtagatt ttgcaaataa aatcggctat
    37261 cctgtcatcg ttcgtccagc ctttacttta ggcggaactg gtggaggtat gtgtgaaaat
    37321 gaagaagaat tacgtatcgt atcagaaaat ggattaagtc tttcccccgc tacccaatgt
    37381 ttgattgaac gaagtattgc aggttttaaa gaaattgaat atgaagtgat gagagattcg
    37441 gctgataatg caatcgttgt ctgcaatatg gaaaattttg acccggtggg tatccataca
    37501 ggtgattcta tcgtatttgc ccctagtcaa acattgtctg accatgaata tcaaatgctt
    37561 cgtgatgctt ctctttcgat tattcgagca cttaagatcg agggaggctg taatgtccaa
    37621 ctcgctcttg atccacatag ttttaattat tatgtgattg aagtcaatcc gcgtgtttct
    37681 cgttcatctg cgttagcaag taaggcgact ggctatccga tcgctaagct agctgctaaa
    37741 atagcgcttg gattaacatt agacgaaatg aaaaatcctg ttacaggaac gacttttgca
    37801 gaatttgaac ctgcattaga ttatgttgta gctaaaattc ctcgctggcc gtttgataag
    37861 tttgaaaacg gagaacggct tttaggcacg cagatgaaag caactggtga agtgatggct
    37921 atcggtcgga atatcgaaga gtctttattg aaagctgtgc gttctttaga aatcggtaca
    37981 caccatatcg aacttcctga gttggcagac atcacggacg atgacctaat ccaaaaagtg
    38041 gttcgagcac aagatgatcg cctgttttat gtagctgaag ctattagaag agggtacacc
    38101 atagaagaat tagcggattt gacaaaaatc gatttattct ttttagataa actgttgcat
    38161 atcgtagaaa tcgaacagca gttgaaacag cagccgttgg atgacaagat attgaaaaca
    38221 gcgaaacaaa acggttttac agacaaaaaa gtggcggagc tgtgggaaat aaacgaagaa
    38281 gccgttcgcc agatgagaaa agaaaatgat ttgattcctg tatataagat ggttgacact
    38341 tgtgcagcgg aattcgaatc aaaaacacct tatttttata gttcctatga atatgaaaat
    38401 gaaagttcac gatcagaaaa gccctcaatt ctagtactgg gatctggtcc tatccgtatc
    38461 ggacaagggg ttgaatttga ctatgcgact gtgcattctg tcaaagcaat ccagcaggca
    38521 ggatacgaag caatcatcat gaacagcaat cctgaaacag tttcgactga cttttctatt
    38581 tcggataaat tatattttga gccattgacc ttcgaagatg tgatgaatgt gatcgagcta
    38641 gaacagccga taggagtaat cgttcaattt ggtggtcaga cagcaatcaa cttagccgaa
    38701 cctttgactg ctgctggtgt aaaaatctta gggacaacga tcgaagactt ggatcgtgcg
    38761 gaaaatcgtg atttattcga gcaagcgctg aaagagttgg atattcctca acctttaggg
    38821 gatactgcta caagtaaaga agaagccgtg gttatcgctt cgagaattgg ttatccagtt
    38881 cttgttcgtc caagctatgt attaggcgga cgtgcgatgg aaatcgttga aaaccagcga
    38941 gatttagaag attacatgga acatgctgtg aaagcatctc cagaacatcc agtactagtg
    39001 gaccgttact tgcttggtgg tgaatgcgaa gtggatgcga tttgtgacgg tgaaacagta
    39061 ctcatcccag ggatcatgga gcatattgag cgtgctggtg tccattcagg ggattcaatg
    39121 gctgtgtatc cacctcaaac attatctgaa tcgatcaaga aaacgatcga agattacacg
    39181 atccgcttag cattaggttt gaattgtgtc gggatgatga atatccagtt tgtcatccat
    39241 gaaaatgagg tttatgtgat cgaagtcaat cctcgagcaa gtcggactgt tccattctta
    39301 agtaaggtga caggaattcc aatggcacaa attgcgacga aagctatttt gggagagaaa
    39361 ctaaagacac ttggttatga aaacggactt tatccagaaa caaaagaagt tcatgttaaa
    39421 gcacctgtct tttcttttac gaaattgcag aaagttgata cttacttagg gcctgaaatg
    39481 aaatcgactg gagaagtaat gggatctgac agacaattag acaaagcttt gtacaaggca
    39541 ttcgaagctt ctggattaca cttgcctgaa tatggagccg tattatttac gattgcggac
    39601 gaaacaaaag aagaagcact agaattagct aaacgtttta cagaaattgg ctatagtctg
    39661 ataggaacga aacatacagc tgcttatttc gaaaaaaatg gtctggtcgt tgaaacagta
    39721 gcgaaaattt ctgaggaagc agcagaaaaa aatgtagtcg acttaataag agatggtaaa
    39781 acacaagtgg ttgtcaatac gattgataaa gatcggggaa atgcttctaa agacggtttc
    39841 atcatccgaa gagaagcagt tgaacatggt attccattgt ttacttcttt agacacagca
    39901 gatgcgatcg ttcgagtgat ggaatcccgt gctttttcaa ctaaccctat ttaacggcga
    39961 gcacatgtta agacaaaaag acgaaaaacc gttgcaggag gggacagatg aaacaagaaa
    40021 tgatggaaat cgttcagcaa aaacagctgg caccaaagat ttttgaaatg acattgaaag
    40081 gtgatctagt aaaagaaatg cagcaaccag gacagttctt gcatattctc gtgccgcgtg
    40141 cagacttgct attacgccga ccgattagct tgaatcatat cgaccacgaa acaaatactt
    40201 gccgtatcat ttatcgggtg gaaggtgagg ggactagagt cttctctact ctttctgccg
    40261 gagaccattt agatgtcatg ggtcctttag gcaatggttt tgatttgtcc aatcttcata
    40321 caggagacga agtatttata atcggtggag gaatcggtgt ccctcctcta tatgagttat
    40381 caaaacagtt agtcgaaaaa ggaattaagc caacgcattt tttaggtttt gccacacatg
    40441 aagtcgttta ttatgaaaaa gaatttaatc gtctaggaga aacaagaatc gctactgacg
    40501 atggaacata cggtatgcac ggaaatgtcg gtaatttatt gttaggaacg aagacggaac
    40561 ctacagctgt ttttgcatgt ggttcaaacg ggttattgaa gacagtggaa cagctgtttt
    40621 cttcgcatca aaatgtccag ttatcattag aatcccgcat ggcatgtggg ataggggctt
    40681 gctatgcttg cgtttgtcat acgccggaag atgaaacagg aacaaaaagt gtgaaagttt
    40741 gtgacgaagg accgattttt aaaataggag aggtaattat atgaatgaga atcctttagc
    40801 ggtcaaactt cctggcctag aattaaaaaa tcctatcatg ccggcaagcg gctgtttcgg
    40861 ttttggcaaa gaatacggaa aatactatga tttgaatcag cttggatcga tcatggtcaa
    40921 ggcaacgact ccaaatgccc gctttggcaa tcccacgcca agagtggcgg aaacaccatc
    40981 tggcatgttg aatgcaattg gtttgcaaaa tcctggtcta gacgttgtta tgcataaact
    41041 tcttccagaa ttagaggagt acaatgagtt acccatcatc gcaaatgttg ctggtgcttg
    41101 tgaagaagac tatgtagaag tttgtagcaa aattggcgaa gcaccaaatg tgaaagcaat
    41161 cgagctgaac atttcttgcc caaatgtcaa acatggcgga attgcttttg gaacagattc
    41221 tgatgttgct tttcaattaa cacaagcagt aaagaaagtt tcttctgttc ctgtctatgt
    41281 caaattgtcc ccaaatgtaa cagacatcgt tccaatcgct caggcaatcg aagctggagg
    41341 ggcagatggg tttacgatga tcaatactct tcttggtatg cggattgacc ttaagacaag
    41401 aaaaccgatt ttagccaatc aaacaggtgg actctccgga ccagcaatca agcctgtcgc
    41461 tattcgtttg attcaccaag tagcaagtat atccagcctt ccaatcattg ggatgggcgg
    41521 cgtgcagaca gtggatgatg ttcttgagat gtttatggct ggagcaagtg ctgtagctat
    41581 agggaccgcg aatttcaccg atccgtatat ctgtcctaaa ctaattaaag agctccctgt
    41641 gcgaatgagc gaactaggca tcgaatcgct ggaaagatta atcaaagaag tgagagagga
    41701 acgtgaacga tgaatcaact agaccaacga ccaattattg cattagattt ttcaactagg
    41761 caagaggtag aagactttct tcgatttttc ccgaaagaag aaaaattgtt tgtgaaaatc
    41821 ggaatggaat tgttctacca agaaggacct gaaatcgtac gttatctaaa agaggctggt
    41881 cataatgtat ttttagatct gaaacttcac gatattccga atacggtcga aaaagcaatg
    41941 cggggattgg caaagcttgg tgttgatctc acctgtgttc atgccgctgg cggtattcgt
    42001 atgatggagg cggcaatgcg aggattagaa gaggggactc cagaaggtgg aaaacgtccg
    42061 ctacttctag ctatcactca gcttacttct acaagtgaag aagaaatgca tgccgatcaa
    42121 ctaatcgaag tttctttaga aaaaagtgtg atccattatg ctagctgtgc caaaaaagca
    42181 ggattagatg gtgtggtttc ttctgcttgg gaagtagaag cgatcaaaga aatggcagga
    42241 gacgaatttg tttgcttaac tccgggtata agaccagaag gaacagtcgc aggagatcag
    42301 acaagagtag tcacaccttc tcaggcaaaa aagatcggct caacttttat cgttataggt
    42361 cgaccaatca ctcagtcaac taatccttat aaagcatatc aaacgattca aacagaatgg
    42421 agctaaccta aaatgaatgt tgaacaaagt atcgcaaaag acttattaga aattgaagca
    42481 gtatttttaa atccaagtaa tccttttaca tgggcaagtg gaatcaaaag cccaatttac
    42541 tgtgataacc gaatcacgat gagctatcca aaagtccgaa aagagatcgc aaaaggatta
    42601 gcaagcaaaa tcaaagaagc ttaccctgaa gtacaagtga ttgctggaac tgcaacagca
    42661 ggaatccctc atgccgcatg ggtagcggaa atactggatt tgccaatggt ctatattcga
    42721 agtaaggcga aagatcatgg gaaaggtaac caaatcgaag gacgtatatt tgaaggtcag
    42781 aagatggttg tgatcgagga tttgatttct acaggcggaa gtgtcttgga agcagctgaa
    42841 gcagcaaaaa gagaaggtgc ggatatcctt ggtgttgcgg ctatctttac gtatgagttg
    42901 ccaaaaggaa aagcaaattt cgaaaaagcc gaaatccctc ttatgacgtt gaccaattat
    42961 tctgtattga ttgaagcagc tttagaagat cgttatatcg atgagcaaga attaactctg
    43021 ctaaaagaat ggaaaaaaga tcctgaaaat tggcagattt aagcgcgctt gatgaagata
    43081 gagaaatccg atttgtgtct ccagaatcat caattggaat agcaaaaatc caaaaactga
    43141 ttgcgagaag catcttttca tcaggtatga aaagaaactt ctatcgtgat cagtttttga
    43201 tttttttaaa attcattttt ctaagactgg ttagcagaaa gaagagaata atctcttctc
    43261 ttttttctat tatatataga ttttctctat tttttggtaa gggagctgtt tcaaaaatgg
    43321 taaagagcgg aaatgttgcg aatatcagtt atttaatgat atgatattcg gaaaaagaaa
    43381 tctcaaaggg aggtaacttt tatggggttg aatgaattta ttttaggatt gaacaattta
    43441 gagacgatca caagggcacc tggatttttt aaatatacag agcatacagt tgcagcacat
    43501 tcgtatcgtg tagcatctat tgcgcaagtt ctaggagaca ttgaagaagc gaatggacaa
    43561 ccgatcgatt ggaaactgct ctatgaaaaa tcattgaatc atgactacac agagcgtttt
    43621 attggtgata tcaaaacgcc ggtgaaatac gcaagcaaag agttaagagg gatgctgcaa
    43681 acagttgaag aaaagatgac ggacgaattc atcaaccaag agattccagc tgagttccaa
    43741 gaagtttatc gccgtcggtt gttcgaagga aaagatgaaa ccgtagaagg agaactatta
    43801 gccatttcag ataaggttga ccttctttat gaatcttttg atgaaatcat caaaaataac
    43861 ccggaaaaag tttataagga gatgttcatt gaggcagtga ctacgattaa aagctttgaa
    43921 catcggcctt ctgttcagta tttctttaaa gagatatttc cggaactatt gaaccaggaa
    43981 ttttatggaa aagaggcgtt tttagaaacc gtctcaacga ttgtaaagaa agaagaaaaa
    44041 taaaaatgat atgagatgca gggggaagta tagttgaaaa agaatatgga tgtcgaatgg
    44101 ggctattctg gagacaaagg tccggaaaat tgggcgagtt tatgtgattg gtttgcgaga
    44161 ggtgcggaat ttccattgca gtcgcctatt catttgacca agggggaaga aaccaaaata
    44221 gaaggagaca cgctttcctt tcattatcaa aagcaacggt ttactgacaa ggaattcaaa
    44281 aacacgattc atttagtacc ttttgaccaa ctcagttatg tagagttcaa gaaagaacgc
    44341 tattatttga cagatattca tttccacttg ccttctgaac atatcatcaa cggaaaacag
    44401 gaagatattg aatttcattt tgttcatatg aacagcaaaa aagagaatct agttgtgggt
    44461 gtcatgtacc agctaatggt acaggaaggt tggctgtata atcaagaaaa tggggaaagt
    44521 tggaacttag aaaaacatga gcattgggta aatccggatg tgttctttcc ggagcaaaaa
    44581 agccatttcc attatattgg ctcattgaca actccgccta ccagcgggcc aatccaatgg
    44641 tttgtaatgg atcatgtagg aaaactgaac caagagtttc ttctcccctt cgatggtcag
    44701 tatgctcgac cgaacaaccg accaatccag cccttgaatg gaagagaaat ttattatttc
    44761 gaggagtttg agcaatgaat attcagcaga tgaaatacgt ggtcgctgta gcaaacaatg
    44821 gtagtttcag agaagcggca aagaaaatgt ttgtctccca gccaagtctt tcagctggta
    44881 tcaaagaatt agaaaccgag ttagggatca cgctgtttac tcgaacgaat cgaggggcat
    44941 atttaacaga agaagggcaa gacttcttaa atcgtgccga gaaaatattg gtccagcttg
    45001 atacattaga aactcactat ctaacgagtg aaaagagaga caatttttcg atcgctgccc
    45061 agcattatga tttcttaggt cctttgaccg cacagctact cgaggaattc aaagacgaga
    45121 tcaaacaatt tcggatcatg gagacgacaa cagcgagagt gatcgaagaa gtcaaagagc
    45181 agcacagtga gatcggcatt atttatatga atgagcataa tcaagctggt atcagaagat
    45241 atttacaaca aggagagcta agtggccgca gcattggaaa attcaaaacg catatttttt
    45301 taaggcaagg acatccatta gctgaacacg cagagatcac caaagaagaa ttgttatctt
    45361 atcctcaagt tcgttttaca caggatggaa ataatttccc gtatttttat gaagacctta
    45421 tcgagatccc tgatcaagaa acagtgattt atacgagtga tcgaggtact ttgatgaata
    45481 tcgttcttga aactgatgct tacgcttcag gatctggtat tgtgattgga gggattcgcg
    45541 agcacttgcg gttaatccct ttggcagaag gcgaactaaa tgaattttat atcatttatt
    45601 cggcgaaacg tccgctcagt gaaatcgctc aaagatttat caaagagctt actcggttac
    45661 tggaccagca gagctaagaa aagcagatga gaacgctgtt tattgaacac cgttgatcaa
    45721 aaaagagggg ttgtgacaga attttgtcac aactcctctt tttgtagcaa acaatttata
    45781 actaattgct gttgtgggaa agtacgcaaa tatgattcta gaaacactca ttgattacct
    45841 gttttgtttc atagctgtaa ttttttcttc ttctggtgtc acgataatcg tcttttggtt
    45901 ttcataaata acataaccag gcttggcgcc atttggtttt cggatatgct ttacctgtac
    45961 aagatcaacc ggcacttggg cagagtatcg gtattttgaa aaataggcag ctaactcggc
    46021 agcttctgta attgtttcat ctgagggctt gtcactttta atgatgacgt gcgaaccggg
    46081 tatatttttt gcatgaagcc aatagtcggt ttttttagct gtcttcattg tcagctggtc
    46141 attttgaaga ttgtttttac caacaagaat gagcgttccg tcagaagaaa agtattgatc
    46201 aggctgagat ggtttcttac gtttttgttt ttttaagttc ttctttttca ggtatccttc
    46261 tgaagtcaat tcttctttaa ttgcttcgat atccattggg ccggcaattt ccagttgaga
    46321 taagacagat tcaagatatt ggatttcatc tttggcttct tggatttgtt cgccaatcag
    46381 cttaacagcg ttcttcagct tttggtatcg atggaaatat ttctgtgcat tttgatttgg
    46441 tgtcaatgcc ggatctagtg ctattttgat tggccgatct tcttcataat aattaggtaa
    46501 aacaacttct tttgcgccgc gtggtacttg tgtcagaaaa gtagtaagta gttcgccatt
    46561 tctacggtaa ttctcagcgt tttcggattc tttgagtgtt tgttcacgtt ttttcaattt
    46621 atttttattt cgttttagtt cattttcgat ttttcgaatc agttctccgc cttgttgttt
    46681 tgcccgatct ttctctgctt tttcgtgata atagctatct aataattgac tgagtgttgg
    46741 ataagctgtt acagcagatg cctgttcgga caatacctga taagcaatcg gtgtgaaaaa
    46801 atctttattt tccgtttcgt aaaaagtagg aataggctga tggctgatgg ctgagaaaaa
    46861 ttcgtcccat acaaccatct tttcattagg acgttccagt aatcttttgg tcagctcttg
    46921 agcgatatcg aaaccgattc cctgaaactg acgttggatt gcttttggat ctagttcagt
    46981 ttgggaaagc cgctgaaaga ttttttcttt ctcggagcta aatgggttca agacctcttg
    47041 ttttggcgga gcaacatatt ctacaccagg aagcaaagaa cgataagtgt tttgagagct
    47101 tccaatgtgt ttgatcgcat ccagtatttt tccagtttct cgattaacta agacgatcgt
    47161 actatgacgt cccattaatt caacgatcaa tatgatattt tgtagatcac ccaactcgtt
    47221 tcgtttagca aagtggaaat ggatcactcg atcattttcg atttgttcga tgtcttctaa
    47281 aatagcgcca tccaaatatt ttcgaagcat catcacgaag ttaggcggat tatcaggatt
    47341 ctgataatcg atttgggtaa tctgtacacg agcataactt gggtgcgcag ataataataa
    47401 tcgatggttt ttgccgcgtg aacgaatcac cagcacgatt tcattttcat aaggctgatg
    47461 gattttcgag atccggccag atagtaacgt ttcacgcaat tcgttgacca tcgcgtgggt
    47521 aaatacacca tcaaaggaca tggttcattc ctcatttctt ttcataagta tattcattat
    47581 aacgaaaaaa agaagaaaaa ccaatttggc cgaatagtaa agcaaaagtc tagaaaaatc
    47641 ttggactaat tacataaaaa acagtgtttc tatcgttgaa atccaaattt ttaggtataa
    47701 taaaaaatag gagaaaaagt agaaaagaag gtgtttgtta tttgggaagt agttaaatcg
    47761 agagtacaag aaatgaatga acatttctca gtgtcattcg cagaaaatcc aagtaagtat
    47821 tttcgacgtc ttgtttttct gctcatctta tcattgctgt taacagttgc aatgttttta
    47881 ttgccggaaa aaatccgaga agagcaagta aatagtctcg tttattcgtc cataccaacc
    47941 agcaaattga aaccttttga ttacagcaaa gctgatcaga aaatcaaaca aagccgggcg
    48001 atatcggtga tgttcagcaa accgaacggg aaaacctatc aggacatgct tgatgttttt
    48061 aatgatcctg agcttatgga agaattgaat cgaccgatct tttattatcc gatcgtttat
    48121 gatgtacatg aactggaaca aaaatataac atacgaacgg atgaagtgac atttattttc
    48181 tttgatggag gaaaagaagc aaatcggata actgcgggta agggaggaat aactgatttt
    48241 gataaggagt tgatccctga actgaatcga cttccgttag ccaatatcaa acaattagaa
    48301 gaagaattgg atagaacggg agtaacttcc caaaattccg agattgctaa ttgagacatt
    48361 ggcgcgatga aacggtgggt ctatatagcg ctatttttaa ttatcagaac aagtgatgtt
    48421 tgatcttcaa aataacaa
//
LOCUS       DAFEPQ010000014        47563 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.014, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000014
VERSION     DAFEPQ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.014"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(113..1360)
                     /locus_tag="LP543_000901"
     CDS             complement(113..1360)
                     /locus_tag="LP543_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL2321082.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(1436..1582)
                     /locus_tag="LP543_000902"
     CDS             complement(1436..1582)
                     /locus_tag="LP543_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HBL2321083.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(1686..1997)
                     /locus_tag="LP543_000903"
     CDS             complement(1686..1997)
                     /locus_tag="LP543_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBL2321084.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(1999..2196)
                     /locus_tag="LP543_000904"
     CDS             complement(1999..2196)
                     /locus_tag="LP543_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HBL2321085.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(2590..4317)
                     /locus_tag="LP543_000905"
     CDS             complement(2590..4317)
                     /locus_tag="LP543_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HBL2321086.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(4310..5503)
                     /locus_tag="LP543_000906"
     CDS             complement(4310..5503)
                     /locus_tag="LP543_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL2321087.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(5690..6334)
                     /locus_tag="LP543_000907"
     CDS             complement(5690..6334)
                     /locus_tag="LP543_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2321088.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(6428..6562)
                     /locus_tag="LP543_000908"
     CDS             complement(6428..6562)
                     /locus_tag="LP543_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBL2321089.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(6564..8696)
                     /gene="feoB"
                     /locus_tag="LP543_000909"
     CDS             complement(6564..8696)
                     /gene="feoB"
                     /locus_tag="LP543_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBL2321090.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(8693..9166)
                     /locus_tag="LP543_000910"
     CDS             complement(8693..9166)
                     /locus_tag="LP543_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBL2321091.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            9567..9791
                     /gene="nrdH"
                     /locus_tag="LP543_000911"
     CDS             9567..9791
                     /gene="nrdH"
                     /locus_tag="LP543_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBL2321092.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            9950..12109
                     /gene="nrdE"
                     /locus_tag="LP543_000912"
     CDS             9950..12109
                     /gene="nrdE"
                     /locus_tag="LP543_000912"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBL2321093.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            12159..13124
                     /gene="nrdF"
                     /locus_tag="LP543_000913"
     CDS             12159..13124
                     /gene="nrdF"
                     /locus_tag="LP543_000913"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBL2321094.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(13213..14352)
                     /locus_tag="LP543_000914"
     CDS             complement(13213..14352)
                     /locus_tag="LP543_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL2321095.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(14661..15374)
                     /locus_tag="LP543_000915"
     CDS             complement(14661..15374)
                     /locus_tag="LP543_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBL2321096.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(15628..16329)
                     /gene="nagB"
                     /locus_tag="LP543_000916"
     CDS             complement(15628..16329)
                     /gene="nagB"
                     /locus_tag="LP543_000916"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBL2321097.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            16563..18095
                     /locus_tag="LP543_000917"
     CDS             16563..18095
                     /locus_tag="LP543_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL2321098.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQINWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(18286..19488)
                     /locus_tag="LP543_000918"
     CDS             complement(18286..19488)
                     /locus_tag="LP543_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2321099.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(19570..20259)
                     /locus_tag="LP543_000919"
     CDS             complement(19570..20259)
                     /locus_tag="LP543_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL2321100.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(20365..20733)
                     /gene="rplL"
                     /locus_tag="LP543_000920"
     CDS             complement(20365..20733)
                     /gene="rplL"
                     /locus_tag="LP543_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBL2321101.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(20786..21289)
                     /locus_tag="LP543_000921"
     CDS             complement(20786..21289)
                     /locus_tag="LP543_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBL2321102.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(21493..21620)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(21699..22388)
                     /gene="rplA"
                     /locus_tag="LP543_000922"
     CDS             complement(21699..22388)
                     /gene="rplA"
                     /locus_tag="LP543_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBL2321103.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(22492..22914)
                     /gene="rplK"
                     /locus_tag="LP543_000923"
     CDS             complement(22492..22914)
                     /gene="rplK"
                     /locus_tag="LP543_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBL2321104.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            23257..23925
                     /gene="sdaAB"
                     /locus_tag="LP543_000924"
     CDS             23257..23925
                     /gene="sdaAB"
                     /locus_tag="LP543_000924"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBL2321105.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            23938..24810
                     /gene="sdaAA"
                     /locus_tag="LP543_000925"
     CDS             23938..24810
                     /gene="sdaAA"
                     /locus_tag="LP543_000925"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBL2321106.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(24901..25920)
                     /locus_tag="LP543_000926"
     CDS             complement(24901..25920)
                     /locus_tag="LP543_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBL2321107.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(26093..26998)
                     /locus_tag="LP543_000927"
     CDS             complement(26093..26998)
                     /locus_tag="LP543_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL2321108.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(27057..27602)
                     /gene="nusG"
                     /locus_tag="LP543_000928"
     CDS             complement(27057..27602)
                     /gene="nusG"
                     /locus_tag="LP543_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBL2321109.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(27703..27873)
                     /gene="secE"
                     /locus_tag="LP543_000929"
     CDS             complement(27703..27873)
                     /gene="secE"
                     /locus_tag="LP543_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBL2321110.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(27897..28049)
                     /gene="rpmG"
                     /locus_tag="LP543_000930"
     CDS             complement(27897..28049)
                     /gene="rpmG"
                     /locus_tag="LP543_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL2321111.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            28211..28855
                     /locus_tag="LP543_000931"
     CDS             28211..28855
                     /locus_tag="LP543_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL2321112.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(28911..29414)
                     /locus_tag="LP543_000932"
     CDS             complement(28911..29414)
                     /locus_tag="LP543_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBL2321113.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(29427..29473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            30011..30976
                     /locus_tag="LP543_000933"
     CDS             30011..30976
                     /locus_tag="LP543_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBL2321114.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(31075..32247)
                     /locus_tag="LP543_000934"
     CDS             complement(31075..32247)
                     /locus_tag="LP543_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2321115.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(32234..33379)
                     /locus_tag="LP543_000935"
     CDS             complement(32234..33379)
                     /locus_tag="LP543_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2321116.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(33393..34317)
                     /locus_tag="LP543_000936"
                     /pseudo
     CDS             complement(33393..34317)
                     /locus_tag="LP543_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            34434..35513
                     /locus_tag="LP543_000937"
     CDS             34434..35513
                     /locus_tag="LP543_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL2321117.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            35545..36138
                     /locus_tag="LP543_000938"
     CDS             35545..36138
                     /locus_tag="LP543_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2321118.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(36248..36475)
                     /locus_tag="LP543_000939"
     CDS             complement(36248..36475)
                     /locus_tag="LP543_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL2321119.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(36578..39166)
                     /locus_tag="LP543_000940"
     CDS             complement(36578..39166)
                     /locus_tag="LP543_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBL2321120.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(39186..39845)
                     /locus_tag="LP543_000941"
     CDS             complement(39186..39845)
                     /locus_tag="LP543_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL2321121.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(39894..40397)
                     /gene="trmL"
                     /locus_tag="LP543_000942"
     CDS             complement(39894..40397)
                     /gene="trmL"
                     /locus_tag="LP543_000942"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBL2321122.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(40618..41484)
                     /locus_tag="LP543_000943"
     CDS             complement(40618..41484)
                     /locus_tag="LP543_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL2321123.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            41593..42234
                     /locus_tag="LP543_000944"
     CDS             41593..42234
                     /locus_tag="LP543_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBL2321124.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(42296..43657)
                     /gene="mgtE"
                     /locus_tag="LP543_000945"
     CDS             complement(42296..43657)
                     /gene="mgtE"
                     /locus_tag="LP543_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBL2321125.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(43675..44574)
                     /locus_tag="LP543_000946"
     CDS             complement(43675..44574)
                     /locus_tag="LP543_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL2321126.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(44576..45373)
                     /locus_tag="LP543_000947"
     CDS             complement(44576..45373)
                     /locus_tag="LP543_000947"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBL2321127.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(45351..46055)
                     /locus_tag="LP543_000948"
     CDS             complement(45351..46055)
                     /locus_tag="LP543_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBL2321128.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            46186..46761
                     /locus_tag="LP543_000949"
     CDS             46186..46761
                     /locus_tag="LP543_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HBL2321129.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            46879..47556
                     /locus_tag="LP543_000950"
     CDS             46879..47556
                     /locus_tag="LP543_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBL2321130.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT        14377 a         9881 c         8312 g        14993 t
ORIGIN      
        1 aatactttat cgtgaaaaag aatgaaatta tcaataattc gttctaactg atttaattgt
       61 ataatttgat cttcacataa tattattgga taactcataa atcctccttc cctcaaaaca
      121 acaaaataac ttgagtagta aattgatacg gatcgttttg atagttcaca aacatattgg
      181 gattcttttt attgatttcc tctatactag ataaacctaa cccttgatgt cccttttttg
      241 ttgttattcc ttttttcatt agagtatcta tagagagtag tatctctcta caactattcg
      301 atatcgaaat ttctatctgt ttatcatctc ggtaaatagc tagagacagt tctctatctt
      361 gactctctat agatgcttca attgcattat cgagaataat tccaagcacc ctaatacagt
      421 caaatattgg aacaggaact tcaataatag tacttggaca ttcgaattta caacaaatct
      481 tattgttatt tgcttggtat agcttcgcta tcaacaaact tttcaaatat tcatttttta
      541 tattttttaa ataacgatat tcaaacttat tggttagata agaattagaa tagatctcta
      601 attcttttat ttctttttcc aatatcgaat catggttttg attagcgatt tcttttaaac
      661 tcagcaataa atttttatag tcatgtctaa atctggcaaa tttttcttga tctttttcca
      721 gtgaatcagt atattttttt agataaacca attcttttct tttcaattgc tgttcatatt
      781 gttctcgttg cctaatagtt attctaataa ataaaaatag aacaacaaac atttgaatga
      841 ttaaaaatat taagacacca aacacaaaat gatcaaatac ttgatattga tgagcaacat
      901 aagaaacaaa taaaccaatt gtaagtaaat aaatcatgaa tatagcagta agtttagaac
      961 tggactgttc aacaatccta tttatagcta attttctata taaccaaata aacatcagca
     1021 aaaacaaaga atcaagcatt atctggaata ctacataacc ataccctttt atcccttcaa
     1081 atgaaaacat atacgtcata accatggaag taataataga tctgaaatca tcaataagca
     1141 tacataatag taatttattt aaaagcacta tattagtttt cttcttttcc tttaatataa
     1201 aataacttcc gattataggt atagctgcgg aaacatcagt aaataattca acaaaaaaag
     1261 tgagtggtat caataaacat acaaaaagat attttctttt gttaagatca gtattagtca
     1321 tataaaataa gagaaaaaag aaaaagtttc ccacgaacat tcttaataga taagcttttg
     1381 tatcgatagt aatcaagaga tcccctcctg attttaggat atcataaaaa aaattttagt
     1441 tacacttttt aaataatgaa aaaacacatt ccactaaatt tgatgccggt tttccttgag
     1501 gttttgttcc agcacctcct ttaatttttt taagctcttg atctgaacat tgtttagcat
     1561 ttagtctatt tttttcttcc attacgattc cccctacaaa ctataaatac ttttatgtca
     1621 ctgattcaat atacctattt ttctaatcaa aaaattttga gacatattta atattataag
     1681 tatcattaaa attgagattt atctccataa tctgctcgat ataaaccatt tattccagca
     1741 gttcttccaa tttcactcaa atttcttatt aattcctctt gatattcagt gaatcttatt
     1801 ttattagtat aagcagtaaa atatagataa ttcaccaatc tatttattaa aggaccggga
     1861 tctaattcat atttttgttt ttctaatttt aaatatacct tttgtaaaac ctcaagaata
     1921 tcagaatatt taggatcttt acttatagat atatcattat ataattcatg aacaatttgc
     1981 ttagcatttt ttttcatttt agcacttccc tggaattgct ccacctaaaa aaccacctat
     2041 agacattcct gcaatacaag tagttgcctt ggcccaatcg accgtacatt tatttttagt
     2101 gcaatacact ccatttccat aatattttcc actatgagtg gtacccccat agataagttg
     2161 tgtctcttta atagacaaaa tttttaaatg tttcataatc taatcacccc taaattaatg
     2221 tttttagact attattctaa cattcattaa atttcaaaaa acaattcctt cttgaaacgt
     2281 agcaaaataa ttcctgaata agaatttaat cacttttaag tatataaact atgtatattc
     2341 tttaatattt tttgagaatg cacttcgtac ataataaact ttaatcacat tagtctttca
     2401 tacctacgat cgatctcttt gactcgcacc cataaattca taatagtttg tcggcgtctt
     2461 tggacgtttc gaagatgagc tacgcattct ctttttttat agacaaaaaa gatagagacg
     2521 aacatggagc ttccaaattt ggatatttga tccaattttg gaagctccat ttatttcgtg
     2581 tctgtctttt tattttttcc agctttcgac taattcttgg ttctctttga tccagtcttt
     2641 tgctgcttgc tgtggatctt ttccattgtt gatttccaac ataacttttt ccatatcttt
     2701 ttctgaccaa tggaaattgt ctaatacttt gtaagcttct ggctggtctt ctttcaatcc
     2761 ttttcgaacc atcgtatgga tctgctcttc gcttcccatt ccgttttcag gatcttctaa
     2821 gtatttcaga tcatatttgg caaacatcca atgaggtgtc catcctgtaa ctacaattgg
     2881 ttcgtgtttt ttgatcgctt gacctagtgc aacggtcatt gctcctgaag aagatgtctc
     2941 tactttccag tctttcaagt tgtcatattt ttggatcgtg ttttcagcag ccgtaactac
     3001 accagctcct ggctcgattc ctatgatttt cttacctgcc tgatcggtta actcatcgat
     3061 cgaatttaca tccatgtagg ctggcactgc taaaccaact tttgctcctg tcaaattggc
     3121 acctaggtct tcgacttgat ctttatattg tgcatattgt gaaccatgag ttttaggtag
     3181 ccaagctgaa accatggcat ctgactcgcc atttgaaaca gatttccaca tgattgaatt
     3241 atctaatggc gtcatcgtta catcatagcc catttgtttc aatacttcac cgactacatt
     3301 tgttgaagca acttctgtat cccattcaac ataagataag ttgatacgtt ttgtttcttt
     3361 ggcagatgaa aaactcccta taccaatcgc acctaagatc actactacta cagcaccaat
     3421 gatcagtcct tgacgttttt tacttttctg tgttgttgct cctgcagctt ttttattgtt
     3481 tggctggttc aaatgctgtg taaaacggtc aatgatgatt gctaagataa ctaatgcaac
     3541 cccgttgaca aatccattcc cgacttgtgc tctttgcaat gctgataata cgccgcgtcc
     3601 taagccgggt gcaccgatca tagaggcaat aacgaccatt gaaagtgaca gcattgttgt
     3661 ttgattgatc cctgccatga tcgtactttt agctaaaggc aattctagtt tgaatagttt
     3721 ttgacgactt gtgctcccga atgaatcaga agcttctact aattctttag gaacttgtcg
     3781 aatccctaaa ttcgtaaaac gaacggttgg tggcagagca aagatgactg aggcaaatac
     3841 ccctggaacc atcccgatac cgaaaaatgc aactgctgga atcaaataaa cgaatcctgg
     3901 cattgtctgc ataaagtcaa ggatcggttt gataatactt tgggctttac tgctttttgc
     3961 catcaagatc cctaatggta ccccaatgat gatcgagatc acactagata aaagaacaag
     4021 tgtgacagta ttcatcaaat cattccatag accttggtta taaataaacc atagaccgat
     4081 taatgtgaac aacgtaagtc ctggacgttt cttagaaata aagaaagctg ctaccgtcaa
     4141 taacaagata aacaaaggag ctggaatcac taacagtaaa tttgtaatcc ccgacatcac
     4201 tgcctgtcca atcgtttgta aaaatgaaaa taggccagaa aaagttgtcg taaaccattc
     4261 tgtgatattt tctacccagt ttgccactgg taattgatag ttcaatagat tattcatgtt
     4321 cactcacctc gcttgtttct gctaatgctt caatcacact acctcggatg acgacacctt
     4381 ttaattttcc atcctctaca actgcaagtg gagtcggtga gtcataaata atattgaaaa
     4441 tatcacttac caccatgtct ttcccaatca ccgtcacatt ttcatctagg aagtcgatca
     4501 atggacgttt ttgcttccta gcttctaacg cttgatctgc agtgatgatc cctttcaggt
     4561 gtctctttct gtcaacagcc atcagcatgc tgacttcttc attacgcatc cttgtcaatg
     4621 caacgtttgg tccatcagat tcgatattcg tcgtcaatgc aggtaccatg atgttttgag
     4681 ccgtaagcac tttggaacga tcgacttctt ccacaaattc acgtacataa tcatttgctg
     4741 gattggtcaa tatttcttca cctgttccga tctgcatgat ttcgccgtct ttcatcaagg
     4801 cgattctgtc cccaatccgt aaagcttcat tcaaatcatg ggtaatgaaa ataatcgttt
     4861 tttgaacatt ggcttgcagg tccagtaatt cgtcttgcat ttctcgccgg atcagcggat
     4921 caagagccga gaacgcttcg tccatcagca agatctcagg atcatttgct aaagctctgg
     4981 ctaggccaac ccgttgttgc atcccaccag ataactgact aggatattgg tctttaaatg
     5041 aaagcaaact agaattttct aatgcttttt ctgctttttc ttgtcgttct tctttaggta
     5101 caccacggac ttctaacccg tattctgtat tttccaagat cgttcgatgt ggaaacagac
     5161 cgaagttttg gaaaaccata ttgattttat gacgacggac ttctcgcaat tcttctttgc
     5221 ttaacttcga tacatcttct ccatcaatat agatactgcc cgaagttggc tcaatcagac
     5281 gattcaatag acggatcaat gtggatttcc cactcccgga aagccccatg atcacgaaga
     5341 tttctccttc ttttacgtca aaattcacgt catacacacc aaccgttgca ccggttttct
     5401 ttaaaatctc cgttttcggc tggtgatcat tcatcatatc taaagcggct tgtgtctttt
     5461 taccaaatat ctttgttaaa tgttttactt ctacttttac caaacaaatt ctcctttttt
     5521 atgtaaattc atttttcttt ctgaaaaaag tgaccacact atgttaacat catgtagtca
     5581 ctttgtcaaa aatgaaagaa atttgcttgt ccaaactgaa aaaaacttgg ctaaatcttt
     5641 gtaaataaaa ggtttagcca agttaatatt tttttaatat ttcacacaat cattctgcaa
     5701 gctgatgctc cgggtaaaaa aagtttgtca tacgctgtag tgtatattca tttccaataa
     5761 aatgaacgat gtcccctgcc gtaaatttag cataggggcc aggtgaaaca agcagttctt
     5821 cattatgaga aattgcgata actgtggcgc cagtcgcctg ccagatattc aactcgctaa
     5881 ttgaacggtc cagatgcatt gctttttctg ataaagataa ttcaaacgga agtaaaggat
     5941 tcatcttatg tattcgcttc gtttgattga ccagctgatc caataattga gaaaaatgat
     6001 tcagctcatc ttgttgcttc tccacacttg ccatgatttc tgcttttgta tcttggatgg
     6061 attgtacatc ttgatattgc aataggaaat cttttgcttt ttccttcgaa gcaacaaatg
     6121 ccccacttcc atgccgtact tccatgattc caagatctac cagcacattg atagccttgc
     6181 gggtagtctc aggagaagca ttgtacgtac tggcaagtgt tgagcgggca tggatcttct
     6241 cacccacttt atatcggttt tcaacgatac gttcggctaa atcaacagca atctgctggt
     6301 agcgcgggcg gacgattttt ttctttccag acattttctt tcctctttct ttttttactt
     6361 tccctcatct tactatgctc tagcaaaata agcaaaaaag gggagaatag cctcttgcaa
     6421 cgactgatca tcgcttgatc tgttcctttt ttactgggca agctgtttga cagtcatcac
     6481 aattcttacc ggattttaaa cgagtataga caattgcgcc tgcacttcca aagattaaca
     6541 cacttaataa gaatgtcgcc atcctattcc tctccttcta atgacaatat agtataaaac
     6601 tggttcttgg attttggctg acggaacaaa acataaacga gtcctatcat cagtaaaata
     6661 gcaagatacg tccctaacgc tacctgtcca ccctccaata agtgtcccaa ctgataaatc
     6721 acaaaactaa ctaaataagc taaaccgcat tgatagccta tggcgatcca agtccatttc
     6781 ttggcattca tttcccgatg gatagcccca attgccgcaa aacaaggcgc acaaagtaaa
     6841 ttgaaggcta acaatgaata agctgctgcg ggagtatatg tgtgttgtag cacttgccag
     6901 atttctctgc cattttcgga tacttccgca tgaccaaaca aaataccaaa tgtgccaata
     6961 atattttctt tagcgaccaa tcctgtaatc gctgcgactg ttccttgcca gtttccccaa
     7021 ccaagcggct gaaaaacaac tgccaggatt cgcccgaaaa aggctaagat actttggtct
     7081 tcagaaacag gttgagcata aaaattataa gaagagctga accagatcac gatactagaa
     7141 acaaagataa tcgttcctgc ttttttaaca aaagacttgc tgcggtcata ggtttgtctt
     7201 aggaccgctg accattgagg aaaatgatat gcgggcaatt ccatgataaa aggagctgta
     7261 tctccagcaa acaaacgtgt ttttttcaag ctgattccag agaaaataat tgcagtcata
     7321 ccaagaaaat aggcagaagg agctacccaa gattgatgag gaaaaaaggc ccctgctact
     7381 aaggcaataa tcggcaactt tgcagaacaa ggcatgaagg ttgtgaccat gatcgtcaaa
     7441 cgacgatctt gctcgttttc aatcgtgcga ctggccatga cgcctggcac tccacatcct
     7501 gtagcaatca gcataggaat gaaggatttt cctgaaagac cgaatttcct gaaaagtcga
     7561 tccatgacaa aagcgatcct cgccatatat ccgcagtctt ccagaaagct caagcataaa
     7621 aacaatataa tcaattgcgg tagaaatcct aacactgctc ccacgcctgc gatgatgccg
     7681 ttcaagatca gctcctgcat ccatcccgct acttgccatt cagctaacaa tctgccaaca
     7741 tgttctggta accatgtacc aaacaatgtg tcattgatcc aatctgtccc cattgttcct
     7801 actgtttgaa tagccagata atagatcagc cacatcacaa atgcgaagat cggcaaggct
     7861 aaccaacgat tcgttgcaat ctgatcgatt ttgtcactca tcgataattg gaaagaatca
     7921 tttgacacaa cacagagtgt acatagttgg gtaataaatt cataacgttc attgaccaga
     7981 atcgtctcac tatcgtcgtc caatagtttc tctgttaact ggatcagttg ctcgatctct
     8041 ttttgctgag aaactgctat ctcgtaatcc tctcttacct tctgatctcg ttcaaaatat
     8101 ttcattgcat accagcgttt cttgccgctt tgttctccta gtacatcttc aatttcatgt
     8161 aaagctgctt ctaaacgtgg atcatatgta cagggaacag gaatagaagg ccgctttttt
     8221 gcagactcct gaatcttttc gattagtttt tcgattcctt gctttttcaa tacgctgatt
     8281 ggtaaaactg gtacaccaag accataggaa agcttttcta gattgatttt ttttccttct
     8341 tttttgatga catccatcat attcagacca atgatcatag gaatgtctaa atccaataac
     8401 tgcgtagtta aataaagatt tcgttctaaa ttggtagcat caataatgtt gatgatcagt
     8461 tcaggctgtt cctgaaccaa ataatctctt gcaatcactt cttctggtgt ataaggagac
     8521 aaagaataga tgccgggtag gtcctgaata tgaatgttcg gatttttttt caacgatcct
     8581 tctttttttt ctactgttac gcccggccaa ttccctacat attgattcga accagtcaag
     8641 agattgaaca agctagtttt cccgctgttt ggatttccga ctaacgcgat attcattctt
     8701 tttcctccct ttgtatcaag attagttcgg cctctgactt tcgtaacgtc agttcatacc
     8761 ctcgcactgt cagttcgatt ggatctccca gaggggctag ttttcggata actattttta
     8821 catttcgcgt caatcccata tccattaacc gtcgcttcac tgcgccttgt ccgtggattg
     8881 ctacgacgat cccagtctct cctgctttta attgatcaag agaagtccag ctttcagagg
     8941 aagcagtctt tttattgaca aagatttgac ctagtatttc ccgacttact gccactcgat
     9001 gactttttac cagtaaaatg gcattgttgc ctgataaatt gacgacgacg attttacttc
     9061 cggcaacgat cccgatattt tgcaaatggc gttttgtttt ttcttctgtt tcgattcgtt
     9121 cgacttgata aacgaaattc tgctttgcct gcgctaaggt ctgcatattg attcctctct
     9181 tgtgaaataa atcatgtatt aaaatcattc attatatgtt ctaattttct tacgtgttac
     9241 gcaaataaat gagaatcatt ctcacataga atactctcgg taattgagaa tgtcaattat
     9301 acttgtgata ttatgagcga atctttagta ttgatagata aaaatgagaa aaaaatattt
     9361 cttcatagtt ttccacaatt ttttctctat taaatttgct attttcccac acgatacttg
     9421 ttttcagctc ttttttcaaa aacacaaaac ttagtattcc acagtttcca caacacaata
     9481 tgtagtgttt gttgtttgac ttttttacta agagttttat actatctatt atagataata
     9541 agaatgtgtt agaggaggca ctcaccatga acgtaaaaat cttttctaaa aataattgta
     9601 tgcaatgtaa gatggtcaaa cggtttttaa cagaaaatca aattgctttt gaagaagtca
     9661 atatcgatga acagcctgaa gcagtagact ggttgaaagc acaaggattc caaagtgttc
     9721 ctgtcattac ttctgatgca gaaacagtta tcggattccg tcctgatcag ttgcgtaaac
     9781 ttgccagcta acatgactaa aagaatcaaa acgtgataca tggatttgaa gggcttgaga
     9841 cagcaccagt ttttgtcgca aacttccaaa tcctttttta aagcttaaaa acaatgcaat
     9901 gatttgcaat caagcatcat caaatacgaa atgaaggttg ggcgtacgta tgagtcttaa
     9961 aaatttacaa gatgtcagtt atttcaagct gaataatgaa atcaatcggc cagtcaatgg
    10021 tcaaatccct ttgaataagg ataaagaagc tttagttgcc ttttttgagg aaaatgtcaa
    10081 accaaatacg atgacttttc tatctattat ggaaaaaatc aattacttga tcgatcaaga
    10141 atatatagaa gcagaattca ttcaattgta ttcaccatca tttattgaaa agctatatca
    10201 atttttggac gagcaacatt tttctttcaa atcttttatg gctgcttaca aattttattc
    10261 tcaatatgcg ttgaaaacca acgacggttc cgcttatctg gaaacttatg aagatcgagt
    10321 agccttcaat gcgctttact ttgcgaacgg ggatgaagaa ttagcacttg ctcttgcaga
    10381 cgaaatgatc catcaacgat atcagccagc tacaccttct ttcttgaatg ctggacgtaa
    10441 acgtcggggc gaattggtgt cttgtttctt gactcaagtc actgacgata tgaactctat
    10501 cgggcgctca atcaactctg ctttgcaatt gtcccgtatt ggtggcggcg tagggattac
    10561 tttatcaaat cttcgtgaag ccggcgctcc tatcaaagga tatgagggtg ctgcaagtgg
    10621 tgtcgttcca gtcatgaaat tattcgaaga tagtttcagc tattctaatc aattaggtca
    10681 gcgtcaagga gctggcgtgg tttatttgaa cgttttccat ccagacatta ttgctttcct
    10741 ttcagcaaaa aaagaaaatg cagatgaaaa gattcgcgta aaaactcttt ctcttggcgt
    10801 gatcgtacct gataaatttt atgaattgac acgtaaaaac gaagatatgt atttatttag
    10861 tccatatagt gtcgaaaaag aatatggtgt cccattctct tatgtaaata ttacagctga
    10921 gtatgatcat ctagttgcaa atccaaacat tcgcaaacaa aaaatcaaag cgcgtgatct
    10981 tgaaaatgaa atctcaaaat tacaacaaga atctggctat ccttacgtaa tcaatattga
    11041 tacagcgaac cgcgagaatc caatcgatgg caagatcatc atgagtaatt tatgttcaga
    11101 gattttacaa gttcaaacgc cttctgtgat taacggcaaa caggaatatg aagtattagg
    11161 aacggatatc tcatgtaact taggctcgac aaatatcgtg aatttgatgc aaagccctaa
    11221 ctttggtcaa tctgtgcgta cgatgactcg tgcattgaca tttattacag atgcatctga
    11281 tatcgatgtc gtaccaccga tccaaaacgg aaataagctg aatcatacga ttggattagg
    11341 tgccatgggc ttacacactt atcttgcaaa agaacaaatg gaatatggct cagaggattc
    11401 attagacttt acaaatattt acttcatgct gttgaattat tggacacttg ttgaaagcaa
    11461 caatatcgct cgtgaaagaa aacaagtttt ccataatttt gaaaaatcag cttatgctga
    11521 cggcacttat ttcgaaaaat acgtaactgg agaattccag ccaaaatcag aaaaagtcaa
    11581 agcattgttt gaaggcattt ttatcccaac tgctgaagat tggaatgctt tgaaacaagc
    11641 agtaatgaaa gatggactgt atcatcaaaa tcgcttggca gtagcaccaa atggctcaat
    11701 ttcatatatc aacgatacga gtgcaagtat tcatcctatc actcgcatga tcgaagaacg
    11761 ccaagagaag aaaatcggta aaatttatta cccagcgcct tatctatcca atgagacgat
    11821 tccgtattac acttctgctt atgatatgga catgcgtaaa gtaatcgatg tttatgcaat
    11881 ggcacaacaa cacgtcgatc aaggcatgag tttgacttta ttcatgcgtt ctgaaatccc
    11941 agaaggcatt tatgaatgga agacaacaac gaagcaaacg acacgtgatt tgaatatttt
    12001 aagacattac gctttccaca aaggaatcaa atccatttat tacgtacgta cctttactga
    12061 tgatgcagaa gaaatcggaa gcaatcaatg tgaaagctgc gtgatctaat atcatagata
    12121 gattcgctta ttcatatttt aattaaagga gcattctcat gtcagaaaca tattatgaag
    12181 caataaactg gaatgaaatt gaagatatca ttgataaatc cacttgggag aagctgacag
    12241 agcaattttg gctagataca cgtatcccac tttcaaatga cctagatgat tggcgtacat
    12301 tgtctcaatt ggaaaaagat acagtaggac atgtatttgg cggtttgaca ctgctagata
    12361 ctgtccaatc tgaaagtggc atggatcagt tgcgtaaaga tgtccgcact ccccatgaag
    12421 aagccgtttt gaacaatatt caattcatgg aatccgttca tgccaaaagt tattcttcta
    12481 tttttagtac actgaatacg aaaaaagaaa tcgaagaaat ttttgactgg acaaatacga
    12541 atccttatct gcaaaaaaaa gctgagcgta tcaatgaaat ctacaaacac ggtacaccgc
    12601 tagaaaagaa aattgccagc gtctttttag aaacattctt gttctattca ggtttttata
    12661 ctcctcttta ttatttagga aacaacaaat tggctaacgt agcagagatc atcaaactaa
    12721 tcatccgaga tgaatctgta catggaacct acatcggtta caaattccaa ttaggattca
    12781 acgagttacc tgaagcagaa caagaaacgt taaaagactg gatgtacaat cttctttatg
    12841 agctttacga aaacgaagaa cgttatacag aagaattata cgatccaatc ggctggacag
    12901 aagaagtcaa gacattccta cgctataatg ccaacaaagc attgatgaac ctagggatgg
    12961 atccactctt tccagatact gccaatgatg tcaacccaat cgtaatgaac gggatttcaa
    13021 caggcacaag caaccatgac ttcttctcac aagtcggaaa cggctatttg ttaggtcatg
    13081 tagaagcgat gaaagacgac gattatttga ttggattgga ataaggattt attttaattc
    13141 aaaaattttt cgaaaaataa aaagagttta gcgtacagat attttaaatg tacactgaac
    13201 tcttttttat gcttattcac ttgtttcttc cagcactttt ggactgtcta acttttccga
    13261 aacatgttcc attcttctca ataaatagat tttccggata tttaatatta tcgtcttaat
    13321 cactgcatag acaggtatgc aaatcagcat gcctaatatt cctgcgacac ttcctgctcc
    13381 aatcaatacc aagatgatcg tcaaaggatg gatattcatt gatttcccaa ataacaacgg
    13441 cttgatcaaa tttccatcta attgttggat cactaaaatc gacaacgcca tatacaaagc
    13501 ttgaaatgga gagataaaca aaccgacaat gattgctggt accgctccaa taaaagggcc
    13561 gacatacggg ataatattcg tgattccgca aatgaccgca agcaacaagc tataaggctg
    13621 atcgaatatt ctcattgcca aaaagctcat tacaccaata atcaaagcat ctaagatcgt
    13681 actgctgata taggcagata atgtttcgtt taattctttt accgtttggc gtaactcttt
    13741 acgaatcgct ttcggaaaga agtgagacaa cgcatcaata aatttgtgcc catccttgaa
    13801 cataaatagc aaaataaatg gaacggtaaa taacaaaata aaaaattgca tcataaatga
    13861 aatgattttt gagacacttg cagataaact gactaatatg atattgaaca aattactaag
    13921 agataaattt gctgatgcta attgttcttc caaattgaaa tttttaaact cttcccgctg
    13981 cgccaattca tttaaccatt tgcttgtttc atctgcatac attggcaagc tctgggtcaa
    14041 ttgaattgtc tgttccatta actgaggcac tacattcatg acagttaaaa caactaaaat
    14101 gattacgacc aaaaacgtca ataaaaaacc gaacactctc gggatccgat gcttttcgag
    14161 aaaaacaacg attggatcga acatataata gataaaacca gcaacaagca atggtaaaaa
    14221 aactgccgaa agcattttca atattggact aaaaatattc ggcatttgta aaataaagta
    14281 cacgccgatg atcgtgagta ctagctccac tgtccagaaa aataattttg attgtttaaa
    14341 acgtgattgc atacatctcc tccaaggtcc tttgatactt ctattctatc atttaacaaa
    14401 gagttacgcg ctgagatttt catttgctta aaaaaaacaa aaaaaataaa catattttaa
    14461 tgagtagacg agtacttaaa caaaaacacg gtttggagta gtgacaaaaa ttatactgca
    14521 gtcccttttt ccaaataaac ggtgttcaaa agcttgcatc ttcgtaataa gcccagccaa
    14581 acgaaaaata taaggaacta tttttatttg gctgggctta ttacttgatg taggacatct
    14641 attttacaac ctcagatcat ttattgcgca atactgacta aatgcaactt tgtgatattt
    14701 ttgtgtacgg acaactcctc gatcacatcg gcataagtga gcccttttgg aagatcaatc
    14761 gtatagatat ttttgtacac tcggtagtca tcaacgaatt ttacgtcaaa attcacatct
    14821 tcgattacga tatttttctc ttcaaaataa tcggtaagaa aatctttggt ttcttttcta
    14881 tggataaatt ggatttctaa ctttttagtc gtaggtacat ggatcacgcg tttcactact
    14941 gtgagtgcaa tagcaatccc gataaaactg atgatcgcaa tggaatagta acccatgccg
    15001 atggcgatcc ctaatccagc aattgcccat aaagaagcgg cagtcgtaag tcccgtcacc
    15061 gattgtttcg tgacgatgat cgttcccgcg cctaagaaac cgacaccact caccacttga
    15121 gcaatcagcc ttgcttcatc tgaacgaatc gttccagcaa gctgtggata ctctaaagca
    15181 tctcttaatg caccactagc gatctcaact tggatcaatg caatgataca tgctcccata
    15241 caaactaaaa tatgcgtacg catacctgct ggacgatttt tatattgtcg ttcaaaaccg
    15301 attgctccgc caaatagcat agcgatccct aaacgtaaaa taatctctgg aattgtcaat
    15361 accatatcat tcatctaaac accctccagt ccaatatatc atgtctgaaa ggagcagaca
    15421 aaaaaagtgc aatttttacc tcttacgaaa aaaggactgt gacaaaattt ttgtcatagt
    15481 cctttttatg aataaacggt gttcaatcag ctgaccctcg gtattactca acaaacggca
    15541 aaatatgggg agctattttt cctttgtctt ctctaatact caggtcaaaa cgctgatttc
    15601 acaacctctt tagcctacta ttgaaaatta caacttactt gctgcttctt catcgatgat
    15661 cacaacaaca ttgtcgtgat tttgcaatac acttgctggc aaatgattcg ttacaggtcc
    15721 ttcaatcatc cctttgattg catcagcttt gctttcgcca aatgccatca agacgatttc
    15781 tttacttttc ataattgaac cgattcccat tgaaacagct tttgttggta catcttctgc
    15841 tttatcgaag aatcgtttgt ttgcattgat tgttgattct gttaagtcaa ctactgaagt
    15901 cgttccgtca aatgaagcac caggttcatt gaaaccgata tgtccatttt gcccgattcc
    15961 taagatttgg atatcgattg gatgtgcatc gatgatttga tcatactttt tgcatgctgc
    16021 ttccagatct tctgcttttc catctggtac aaacgtttct ttgaatggtt ttttatcaaa
    16081 taattggaca tccatgaaat gacggtagct ttgttcatct gaaccgccta gtccaacgta
    16141 ctcatctaag tttacagaag tcatttcact gaaatccaaa tcgcttgaag tcatttcttt
    16201 atataaagta actggtgtgc ttcctgttgc caatcctaat acttttgcac ctttgttcat
    16261 accttctttg atgatttcaa atgctttttt tccgccttct tcagcgtttt ttacacgaat
    16321 gatttccata gtgattgctc ctttatattt tggtatagcc caattatagc attctttttt
    16381 tctttttaca ataattagta tagaccaaat tgcatttttt tcattttctt gacaataaat
    16441 aggaatccgt gttaggtttc ataagatggt tcaatcaaac aatccatgtt gcatgaaaac
    16501 caagcaagtg attcactgtg gacgctttat gtctgcagtt tttttatgaa aaaggagtta
    16561 ttatgagtat actgacatta aacaacatta cccagcaatt tggcgacaaa atcctctatg
    16621 aaggtgtcga tctgcaatta aatgctggcg aacatcttgg gctgattgga caaaacggag
    16681 ccggtaaaag tacattgatt aaaatcatca ctggtgagat cttaccagat gacggacaga
    16741 tcaattggca aaaaaatatt cacaaaggtt acctcgatca atatgtggaa gtcgatgaaa
    16801 cattaacaat cgaagaattt ctaaaaactg cgtacgcaaa agaatacgaa aaggaagaaa
    16861 aaattgcttt tctttaccaa acatatagcg agaccttcga tgattccttg ctggaaaaag
    16921 ctgggaaact ccaaacggaa ttagaccaag gagtgtttta tcaaatcgac accttagttg
    16981 ctgagatgag cagcgggttg gggatcgatg tcttaggttt agattcactg ttgaaaaatc
    17041 taagtggtgg acaacgttca aaggtgattt tagcaaaact acttttagaa aacccagatg
    17101 ttctgatctt agatgaacca accaatcact tagatgatca gcatatccaa tggctgacgc
    17161 agtttcttca ggattttgac ggtgcgtata tcgtgatttc ccatgaccaa gaatttcttg
    17221 atcagatcac cacccatatc gcagacatcg agtttggaaa gattactaag tatactggcc
    17281 atctaaaaca agcattgaaa caaaaggaac aaaatcgcga aagctatttg cgccaatatc
    17341 atgctcaaca aaaacaaatc gaaaagaccg aagcttatat tcgaaaatac aaagccggtt
    17401 cccgatcaac gatggcaaaa agccgacaaa aacaattaga taaaatcgaa cggctgacgc
    17461 ctccagcttc agcaagtaaa gctgtctttg attttccatt ctcgcctatt gtcacgacat
    17521 tagcagtgga aacaactgat ttagtcatcg gatatgatcg accgctactt gcacccatca
    17581 atttgaccat ccgctttggt gaaaatattg ccattcgagg cttcaacggg attggaaaat
    17641 ctactttatt aaaaacactg atcggtgaga ttgaaaaaat aagtggcgat ttccacttcc
    17701 cagataacac aaaaatcaat tacttttcac aagatctgta ctgggaaaat ccattagaaa
    17761 caccactaca atatttaagc agtcaatttc caaaagcgac tatcaaagaa ttacgtcgcc
    17821 aactagctaa agcagggtta gttaatcaat tagcaagtga acctctcact acattaagtg
    17881 gtggggaaca aactaaggtg aaacttgcac agttgacaat gaaccaaggt aatttactga
    17941 ttctcgatga accgacgaac catatcgatc aagaaacgaa agaaagctta caagacggga
    18001 tacagaaatt tccaggaaca gtaatcatcg tctctcatga acaagaattt tatcaagatc
    18061 ttgtgcaacg agtcattgag attgaaccaa aataaacgtt tgattgaata gttgaaaaaa
    18121 aggagctgtg acaaaagttt tgtcacaact ccttcttctg aataaatggt ggccaattag
    18181 ctgaccttcg gtattagctc aaaaaacggc aaaatatgag gagctatttt tatttggcta
    18241 ttcttattac ttgatgcaga acagcttttc acaacctctt ttcgtctatt ttttattttc
    18301 gagatttctc tttcctttaa aaacaactgt ttgaaagaag aacgcacatc cttgaaaaac
    18361 aagcccgcta accgttacac ctaaccagcc atactgctgc cacattgtcg tccctagcaa
    18421 tgaaccagcc gcacctccaa taaagtacat aaacataaat acagtattat ttcggttgct
    18481 tgctgcttca cctagccctt gtactcgtgt ttgattggcg acttgaccaa attgattccc
    18541 gacgtctaat aaaataatag ctaaaagcaa aatggccaaa tggcttccgc caaccaataa
    18601 agcaataaag cttattgttt gcataacaag tcccatatac acgatttttc tttcagaata
    18661 agaatctgcc agtcgtccca cgataggtgc agcaactgct ccagacaaac caaaaatcgc
    18721 taatatccct gcttcgaatg ttccccaatt gtaggcggga ctgctgatat aaaaaatcaa
    18781 cgtagaccaa aagatcgaaa atgttccaaa catgaaaaat ccactgactg ccgcttctct
    18841 taatagacgt tgagaagcaa ctaaatgcgg caaactttta agtgaatcaa ggtatgaaac
    18901 agctgtacta ctttgctgga cagaacgcgg cattttccag taaagcagaa gtgtaagaat
    18961 tccgactgca cataaagcaa ataaatagat catccgccaa gaaaaaagac tggcaacaat
    19021 accagatacc gtccgagaaa gcaaaatccc cacgagtaaa ccgctaagca atgtacccat
    19081 gaccttccct cttgcttttg gtcccgctag taccgcaccg taaggaatga tgatttgcgg
    19141 cacgatcgaa agtaatccta ttaaaaaaga agcgcatgca aataacgtaa acgaaggcgc
    19201 aaaaaaagcg gccaacagag agacggcaga taacgctgcc attcgaatga tcaattttgg
    19261 acgatccacc acgtccccta aaggaactaa aaacagtaac cctaacgcat agcctaattg
    19321 ggtcaacatc gtcaatatcc cgactgcact tgtgctaaca gataaactag ctgctacttt
    19381 tgttccaatc ggctggatgt aatacatatt ggcaacaaca acgccacaag tgattgataa
    19441 tagtaaaata agcgaagttg ttaaaggttt tttttcatta ctcttcattg atttccccca
    19501 tttcctatct tagtttacaa cttatggaaa agattggaaa tagaaaaata caactattcg
    19561 ccacacttat tatttgtgct tttgttttcc ttgttttttc ttgcgatctt tataaatgaa
    19621 gacaactaac cctactagca cagtaacact tgcaaccaac gtgactgcag ttttgttata
    19681 tccgtgaaac agagcttctc caccacacgc atacaacaca gacgaaggga tgactccaat
    19741 cacgatcgac aatatgattt gctgtagagg caatttcatc gcatctgcag caaaattgac
    19801 catagaagat ggaataaccg gtatcatata gcctaacatt acacctattt ttggatgttt
    19861 catttgtcgg attgcttgca cccaatgatt cgtttcggtt ttcttatcta aaaattttaa
    19921 gcgatgcata aggaaaattg cagtgatgtt accaagagca ttacccaaaa tgttgatgat
    19981 cgcacctgtc agtggtccaa agctcaaacc agcaatgact ccaactactg aagtaggaat
    20041 acctggaaca gcacacatca ccgctgtcaa tacgatcaag agtatcgatg caaaagtccc
    20101 atgcgaacga atatgtgcga gtagctctgc cttactcgct ctcggattta aaaataattg
    20161 gatgtctgtc tgatattcta aatacaaccg atagcccact accagtatca gaatgattcc
    20221 caacgagatc aatatcaatc ggattatttt ctttttcata tttcctcctg aaaaaaatct
    20281 atattcatta aataatagtt taacataaaa acaaaaaagc accgaaatcc aacataggat
    20341 ctcagtgctt ttaaatagta aaaattattt aacagttaca gatgcgccaa cttcttcaag
    20401 tttagctttc aattcttcag cttcttcttt agatacgcct tctttaactg gtgcaggagc
    20461 gccatcaact acagctttag cttctttcaa gcctaaacca gttgcttcac gaactgcttt
    20521 gataacttta actttttggt ctccagctgc agttaattct acagtgaatt ctgtttgttc
    20581 ttcagcagca gcagcgccac cagcagcagc tacagctaca ggagcagcag cagatacgcc
    20641 aaattcttct tcaatagctt taactaggtc gttaagttct aggatagttg cttcttttaa
    20701 ctcagcaacg atgttttcaa tgttcaatgc cattataatt tcctccgttt aattgttctt
    20761 ttttatttaa tgctttatgc ggcaattaag ctgcatctcc atcttctttg tctgccactg
    20821 ctttgacagc gtaagccacg ttgcggactg gcgcttgtag tacagatagc aacatagata
    20881 gaagtccttc gcgatttggc aattttgcca atgctgtgat ttcttccaat gaagagactt
    20941 taccttcgat gataccgcct ttgatttcta atgcttttgc ttctttagca aattcgtcca
    21001 tgattttcgc tggtgcgact acatcttcgt tactgaatgc aacagctgta ggtccagtga
    21061 atacttcgtc catgccttca aggccagctt ttttagctgc acgtgaaaga atagaatttt
    21121 tgataacttt catttctacg ttagcatcac gaagttgttt acgtaaacgt gttacttcat
    21181 caactgttaa tccgcggtag tcaacgacta ctactgatgc agctgctgta aatttttcgc
    21241 ttacttcatc aacgattgct gctttttttg cgataattgc ttcactcatt tttgagtttc
    21301 acctcctgaa ttttgatgga agagtttttt gcactgaaaa ggcaagtcac ttttatagtt
    21361 actttgctca accagtatta ttcatcacct attcacttcg ttcatagtga ttctactcgc
    21421 tttgctcgaa aggaaactcc tttcgctttg ctcaaccagt attgttcatc atctcttcac
    21481 ttcgttcata gtgattcaca ataaaaaact ctatgccacc gcagacatag agggactatt
    21541 gaatatattc aataaacgtc ctcggtagga aattaaggct tgatgccacc tactgtcttc
    21601 ggtacaagtt aattattaac ctcaactagc ttagcatgct aaactagttg agtcaagaat
    21661 tatttttcag ttggatcaat caacattcta atatttaatt agaaagaagc ttggtcaaca
    21721 tggataccag gtccaaatgt tgttgtgatc gttacatttt tcatgtattg acctttagct
    21781 gctgatggtt tagcttttag caatacatca tggattgctg caaagttttc aactaatttt
    21841 tcatcgtcaa atgaaacttt tccgattggt acatggatat ttccagcttt gtccacacgg
    21901 taagttactt taccagcttt cacttcgttt actgcttttg ttacgtccat tgtaactgta
    21961 ccagttttag ggtttggcat taagcctttt ggtcctaata ctcgtcctag acgacctaca
    22021 gtagccatca tatcaggtgt tgcaacaact acatcaaagt caaaccatcc gttttggatt
    22081 ttttgaacca tgtcgtcgtc acctacgaaa tctgcaccag ctgcttcagc ttcttttgct
    22141 ttgtctcctt tagcaaaaac taaaacagtt tgtgttttac cagttccgtt tggtaataca
    22201 acagcaccac ggatttgttg gtctgctttt ttagggtcta cgttcaaacg gtatgcaact
    22261 tcaactgttg catcaaattt tgcaatgttt gtttcttttg ctaaagctac tgcttcggca
    22321 acaggataaa ctttacttga atcaactttt tttaatgcat cttgcatttt tttgctcttt
    22381 ttagccattt tggttcctcc ttgattgtgg ttataacggt tgtacctccc acgtgttcta
    22441 tatctttcaa tatagagcca actgagaagc tgctgtctta gggtgtatag attattctac
    22501 agtgatcccc atgcttcgtg cagttccttc aaccatgcgc attgctgctt caacgttagc
    22561 tgcgtttagg tcttccattt ttaattcagc gatttctctt acttgatcgc tagaaacttt
    22621 cgcaacttta tttttgtttg gttcgcctga acctttttcg attttagctg cttttttcaa
    22681 tagaacagca gctggcggtg tttttgtaac gaatgtgaat gaacggtctt cgtatacaga
    22741 aataacaacc ggaatgatca aacctgcttg atcagctgta cgagcgttga attcttttgt
    22801 gaatcccatg atattgatac ccgcttgacc tagtgcagga cctactggtg gagctggagt
    22861 tgctttacct gcaggaattt gcaatttaac gattttttct actttctttg ccacgagaca
    22921 tacctcctta agtccgtgat gtggttaatt ggagatgcat atttctcctc ccacgtttta
    22981 tctgatctga aaaaatccag acaaataatg tgcaaaacaa tgcacactgg tacagtatag
    23041 caaatttatt tgttttttca agattttttt ctgctatttt tacattttgc ttgaataaac
    23101 gctaaattta ttctaaaaaa acaacaattt ttcgttctca tttcttcttt tatgttacac
    23161 ttgcgctagc atggttcaac agatgaaagc aaacaataac acggtttttt agacccttgt
    23221 acaaagattc ctacataaca aaatgaggtg gagaaaatga aagatttaag gtatagaagt
    23281 gtctttgata ttattggacc tgtgatgatt ggtccaagta gctctcacac tgccggagct
    23341 gctagaatcg ggaaaatcat acgtagtata tttggcgaac agcctgattc tgtagatatt
    23401 tatctatatg aatcatttgc aaaaacctat cgggggcacg gaacagatat tgcattagtt
    23461 ggcggcctgt tagggatgga acccgatgat gaacgtttag ctgattcttt gaaattggca
    23521 tatgaagccg gtatggaagt cttgtttgtt cctaagagtg aaaaagccga acatccaaac
    23581 tctgtaaaga tgcttctaaa aaaaggaaca cgaaagcttt cagtcactgg catttcgatc
    23641 ggcggtggca atatccagat ttctgaattg aatggattca aaatttcttt aagcatgggt
    23701 actcctacct tgatcgttgt ccaccaagat gttccaggta tgattgctaa agtgactagt
    23761 atactttctg agaccaatac gaatatcggc actatgacag tgacacggga atcaaaaggt
    23821 gaaaatgcca ttatgataat tgaagtagat gatcctcaag tagaagaaac ggtaaaaaga
    23881 ctcaaacagc tgccaaatat cgacagcgta aattatttcg attaaggagc taaaaaaatg
    23941 tttttatcga ttgaagaatt agtagatcaa gcaaaagctt atccaagtgt agcagaattg
    24001 atgattgcag ttgaaatgga aatgagtggc cgttcccgta gtcacatcat cgagatcatg
    24061 gaaaaaaacc tctcagtaat ggaacaatca atcaacgagg gaaaagaagg tgttacctcc
    24121 gtcacaggaa ttaccggtgg tgatgcaaaa aaattagatg attatttaaa tgaggcagac
    24181 tttttaagtg gagaaacaat cttaaaagct gtacgcaatg ctgtagctgt taatgaagtc
    24241 aatgcaaaaa tggggttgat ttgtgctaca ccgactgccg gaagcgcggg tgttgttcct
    24301 ggtgtgctga tggctgtccg agatcggctt gatttgacac atgaacagca attggaattc
    24361 ttatttaccg ctggcgcttt cgggttagtc atcgcaaaca acgcttctat cagtggtgca
    24421 gaagggggat gtcaagcaga agttggttct gcaagtgcaa tggcagcagc agctttggtt
    24481 tgtgcaaaaa atgggactcc tcatcaagca gcacaagcag tagccattac attgaaaaat
    24541 atgatgggac tgatttgcga tcccgttgca ggcttagttg aagtcccgtg tgtcaaacgt
    24601 aatgctttag gctcttctca agcttttatc tctgctgata tggcattagc gggtatcgaa
    24661 agtgtcattc cgccggatga agtcgttgct gcaatgtacc aagtcggacg tcaaatgccg
    24721 tcgattttca aagaaacagc agaaggtggc ttagctgtta cgccaactgc taaacgactt
    24781 gcggaagaga ttttaaataa gagggcgtga taaagctcct taatttcgag tattaagaga
    24841 ttagttatct tcataacaaa aaacgaagag aggactaatt gcccttctct tcgtttttta
    24901 ttaatccatc aatttcgtat tttttacttc atcgatcgtt tttgttcctg caagttgcat
    24961 cgtgatcgac aattctttgt tcaggtgttc aaaaacagat gttactcctt ctgccccacc
    25021 tagattcaac ccataaatca ctggtcggcc aatcgcaact aagtcagcac cacttgccag
    25081 agctttgaag acatgctctc ctcgacggac accagagtcg aaaatcactg gtacacgttt
    25141 gttgactact tcggcaattt ttggcaatac ttcaaaggac gcaggacctc catctagttg
    25201 acgtccacca tgattggata cccagatacc atctgcccct gcagagatag cgattactgc
    25261 atcttctggc gattgtattc cttttacgat aactggaaga tgggtgattt ctttgattgt
    25321 tttgatgtca tctggtgtca agccttgttt tgcagctgca taaatttctg cgattccttt
    25381 tccttcgcca ttaccacttg cactttgttc gctatacgca gctaagttag gcattggtaa
    25441 tggaaattgg aattgattga tgacgtcttc ttcacggtaa ccaccgagcg ttgaatctgc
    25501 cgttaaaatg atagctttag cccctgcttc tactgctttg ttcaaaatga actcattgaa
    25561 tctgtcgtct ttactcatat ataattggaa aaactgcggt gcgcctggcg ctgcatcggc
    25621 tgcgtctttg attgtcgtat ttgcataagt gctgatggaa aagattgatc cagcagcagc
    25681 tactccttta gctgtatcag cttccccttt ttcatgtgcc aatccttggg ccgctgatgg
    25741 cgcttggata atcggtgtat ctaattcaat tccgaaaaca gatgtatgca aatcggctga
    25801 gtcgattccg cgtaatatcc gtggcatgat tttctttgta ttaaaagaag tcgtattttc
    25861 tttcatcgtc cactcatctt cagaaccacc gcgaatatag ccaaaagctc ctgcttccat
    25921 gcgctctttt acacgacctt ctagttaggc aatgtttaca atatcgattg gatgttcttc
    25981 attacttgta tgataagtca tagtagatcc ttctttcatc ttcttgatta catataaatc
    26041 atactaaact tacaaaaagt aagcaaacat ttactctcag atacttttat cgctattctt
    26101 tccaaataaa tcgacttaca agctgatcta cttcttggtt ttcatgaagc atactatgag
    26161 aggcattttc acctgtcact actttttcct ctatttcatt tccgcgttgc ttcaacaacg
    26221 catagaccgc taatgcactg tttaacggta ctgttccatc actcaagtct gtttcatcta
    26281 gttgaccagc aatcaagaaa aatcgagtgg ttataggaat attaccgatc atttgctggt
    26341 aatcttgata gcggttagaa acgacagcag gcccctttgc taactcatct gtcagagact
    26401 gcgcactatc atcgacaaaa tcattaaaag gcgcgccaat tgctgttaat gtctgaactt
    26461 gtggcaatga cgtatcctgt ccatacgttc ctaaatagcg tagtgcacta actcctccca
    26521 tggaatgccc cactatgttg actttatcta tgccgtagtt ttctttcaga tacaaaagac
    26581 aagttttgat ccattctgct tgattccact cgttattttt attatcttca aacaatactt
    26641 ggaccatcgg attgtctgct tttccagaca aatctcctgt tgcttgtatt tgtccatctg
    26701 ttcctactgt taaaaccaat tcttttactg cttgctgatt tttttccaaa cgccgcaaca
    26761 tccctttaaa ggaattttct gtcccgctgt agccgtggaa aaaaagtgtt ggaatagtgg
    26821 ttttttctat cactttactg gattctgccg ttttttcttt atcagaaact tcagtagtat
    26881 ttatcttcgt cgtctggcaa ccacttaacg caaacaaact gataaggact aaagctgctg
    26941 cagttgcacg tttgcataaa tttttgattg gttctgtcat acacctcttc ctttccatat
    27001 agaaaaaaga aagacttgaa aggccaatca tatgatcgac atttcaagtc tctttattaa
    27061 tcgattttat ctacttgatc gaaatcaagt tctgtactgg tttcacgtcc aaacatatcg
    27121 atgttgactt tcaatttttc tttttctccg tcaacttctg ttacttgtcc ttctaatcca
    27181 gcaaatgcgc cttcgatgat tttcacgact tcgccaactt ctacttctag atcattttgt
    27241 cgcaggctca taccgataga acgcaagata tgattgactt cttctggaag taatggtgct
    27301 ggtttgcttc cggctccgtg tgaaccaacg aatcctgtca caccaggtgt gttgcggaca
    27361 acataccatg aagcgtctgt catgatcatt tcaacaagta cgtatcctgg gaatgtttta
    27421 tgaacgattt ctttttcttt cccatttttg acttcagttt ctttttcttc aggaacaacg
    27481 acacggaaaa taaagtcggc cattttcata ctttgcgcac gagattcgat atttgctttt
    27541 actttgtttt catatcctga atatgtatgc aagacatacc agtttttttc aaaagattcc
    27601 attgaaggct cctcatttca ttgtaaaata aaaaaacctc agtacacccg aagtttttct
    27661 ttcgcttcat tatagcacta aaagtacgtt gtggctagcg agttatttta aaatccagcc
    27721 aaataagctt tggatggctg tatccattac gtaaaacatc aatccgaaaa taatagaagt
    27781 ttcgatgact actaacgtat ctttacggag ttgttttctg cttggccaag ttacttgttt
    27841 gatttcatct tttacgcttt ttagaaattt catgagacaa cctcccttgc tactgtttat
    27901 ttcgtttctt tatgtaatgt atattgatta caatatttac aaaacttatt gatttccaaa
    27961 cgttcgccgc tttttccttc actgactgat tttgtgtagt tgcgagaccc acaaacggaa
    28021 caagcgagtg ctgctttttt ctttgccatt tacttctgcc cccttgctga gaatcagact
    28081 tatacttcat aacagtatca cgtaactttt tacttgtcaa tttgtgaaat aaactatcta
    28141 ttgttttgga taaaattatg atatgattaa tttgaaatta agctaaaatg agacaattgg
    28201 aggtatagcg atgctattaa aaacacttgt atataagaaa aaagatctga caaccgttac
    28261 agagactagt acattagaag aagcattaaa aattttagaa acttccggat accgttgtgt
    28321 gccgatctta gattcttcag gtaatatttt ccgtggaaac atttacaaaa tgcatatcta
    28381 tcgtcataaa gcaaatggcg gtgatatgac gcttcctgtc actcatcttt tgaaaaacgc
    28441 tacaaagttc atttacctgg atacttcttt cttcaaagta ttctttacga tcaaagagct
    28501 tccttatatt gctgtccttg acagtgaaaa tcatttttat ggtattttga cacatagtac
    28561 gttgcttaat atgctctctc aatcttggag cgtagaacaa ggaagctatg tactgacaat
    28621 tgcttccaca ggaaagcaag gagatttggc tgctattgca aaaatcattt caaaacacag
    28681 cagcattgcc agctgtatta ctcttgatat cgaaaaagac gaatacattc gacgtacatt
    28741 gatcacgcta ccggctaaca ctgaagcgaa agtatgtgaa acgatcgtca accacttaga
    28801 gaaaaagaat tataaagtgg tagaaattga aaatttgcaa aaccaagaag cataatcaaa
    28861 aagcgaaaaa agaaaacagc taatctgctt tcttttttcg ctttttataa ttagacacgc
    28921 ttttcaaaat caagcgcgcg atctaaataa tacatcaatg gtgctgaaac agccatgacg
    28981 ataaactcac ttaatgcaag cgtgccatag gttgtccaaa atgcttggcc acctgttgga
    29041 actaacatat aagcaatcag tcccatgctg atggtaaaga agatcgtgtt caatactaaa
    29101 cgcatcttta catttttcac tttgttttgc aataaagctg tcaggcctaa agaaagcaat
    29161 gtttggccgc caccaaaaac cacatccatg ataccaaaac caaagaaggc attatagacg
    29221 atgaccccgc ctaaaactcc ccaaagcaat ttacggttaa agacaactaa atgattcaaa
    29281 ctttctgaaa tacgaaactg gatcggccct gaggatacag gcgcaactaa aatagttaac
    29341 gctagataaa gagccatgat gatgccattt acaacgatta cggataaccg ttgtctgctt
    29401 ttcgtttttt gcattttctc tcctcttttc tcctagtttt tttaacgcgg gatggttgat
    29461 gaaccgcggt tatgtgaaac aaacaataat tataacagac ttttcattta tttcaatccg
    29521 tcttatctgc ttttaaaaaa gcaaaagctt aaacaatgta aacgtttgct aaaaatcgtg
    29581 ggatatggta taaagtaagt gtaaggggtc ataagaattc ccagtaaagc agacgaagta
    29641 ggatctgcaa gaacagaatt gttgtgaaac ttaaataaat aaaaaaggat gtgggaattt
    29701 gagcaacctc ttactagaga acggtctagc aagcagctga agatctaagt ggacatgccg
    29761 aaccttggaa cttctcctta cgtaagtaat ggatcatgcg cttgctaggc ttttttgtgt
    29821 ttttcggtct tcggaccttt ttcattttcc tagacttctg ctataataac caagaattaa
    29881 ttttgtaaca gatttttttg aaaaaacaag tttctttcat tcaaaaaata gaaaaagctt
    29941 gctaattatt ggttaaccag caaacaggag tattaaaata tcttgtttta ttgaatagca
    30001 ggaggaaatc atgaaattcc attatcatat attgatcaat ccatcggcag gcagcggaaa
    30061 tggctataaa gtagcagaaa gaattttacc ggtattaaaa aataagcata ttgactatac
    30121 acttcattat agtgaataca aaggacacga tgctgagatt gctgaaactt tggcaaaaga
    30181 aacattgatt ccttggatag aagaagaaca gaaaacaatc gatactttcc cccttttgat
    30241 catcgtagga ggtgacggca ccttacatca ggtacttgat actttctatc agatggaagt
    30301 agaattccca gttgcttata ttccagcagg ttctggagat gattttgctc gaggggcagg
    30361 ccttccaaaa aatcccaaaa aaggacttca gctgatttta gcagctcaat caccagaaaa
    30421 agtccacata atggcttatg aagaaaaaat aagtgaaaaa aaaggaatag ctgttaataa
    30481 tttcggtata ggattagatg cagctatcgt ccatgcgaca aatcattctt ccacaaaaaa
    30541 gcgattgaac aaatacaact taggatcatt ttcgtattta ttctctattc tccgtgcttt
    30601 attcactcaa aaaggatttc caattctggt agatgcagga ggaaaacaat taagtttttc
    30661 aaacgcattt ttatgtacag caacgaagca tccttttttt ggcggaggaa tcgcgattgt
    30721 tccaactgct gatgcgtcta aaccagtcat tgattttgtc gtggtcgaac gaatcaattt
    30781 ctttaaaatc ttgtggctca ttcttttact tatacagaaa aaacaattga aatcgaaata
    30841 tttccatcac tacacaacaa acagattacg tatcgtttca acaacaccgc aacatggtca
    30901 agaagatgga gaagaaatgg aaaaacgacc ttttgatatc acaataacca caactaaaca
    30961 gttgttttgg tgttaataaa agaggttttg tcagaagcta catctggaac accgtttatt
    31021 cggaaaaaag gactgtgaca cgacttttgt cacagtcctt tttttactat gcagttaatt
    31081 ctttgcccaa ttgggttgcc atcgatagtt tctttcttta cccaaaataa attggatcat
    31141 tgaaaacacc gcagcaaaag caactaagac acaaccattg aaccaaaaag attctgcaaa
    31201 tgtttgattg taactgagtg tttcactgat caagttatta ttttgaacaa accaatatgt
    31261 tggtgtgaat gccgctattt tgctaaccat ttctggcaag tattctgctg gtacaaacac
    31321 cccaccgata aagcaactgc ctgtgataaa aatatctttg atcccaccgc tggtttctga
    31381 gttttttacc aatgaagtga tacaagccga aaagctaatg atcggcaata aaaacaagaa
    31441 agtatttaaa atatgatacc ataccgttat atcccacgag gtgtggatca ctaacagaac
    31501 cattagaagt aagaagccac tgaacaacag cagcgagtaa ctaatcaaac taaaggttac
    31561 tttgcgattc aagtttcgtt tggtaatggg cgcactctgg tttcgtttac tgatctcttt
    31621 gcgattaaat gctagattga tgacgctgaa accgctaaaa atggatagaa acaacccata
    31681 agacaagaaa ttaaaggttg tgcccgtcaa cagctttttc atccgctgac tatatcccgc
    31741 acttagctca acttctccag taaccgagag tgccatttgt gtctgttcca tagctgcatc
    31801 gattgtttta tcttcttctt gaaacagaat cagtgtttgc aaaaaggtgt tcacttgctg
    31861 tgtaacaact gttttagaaa aggcatctgg gcgactctgt atcttgatcg tcggcatctt
    31921 tcctacttcg acttgttcgg taaagtctgc aggcagatgt aagataatgt ttacacgatt
    31981 aaaataaaga gcatcatcga tttctttttg tgaggttcca tccaatgtaa cgactgtttc
    32041 cttgtcattc aaatatttac gaaggccatc gactacaggc gactcttctt cactaatcaa
    32101 ggcgattttc gcatcttgca gagaggtatt ttcaacatca atattttgcg caaaaaagaa
    32161 tgtggcaaca acagtgataa ttacacctat cagtaaattc gatttatttg ctttaagcaa
    32221 tgtccaaatc gttttataaa gcgtcataac gcacccctct ttccatccaa gcatttccta
    32281 acgcaaaaag gaaaataaat ccgatcaacc atagaagatt ggtataaaaa ctagccaaat
    32341 cagtgtagaa ataaagttta tacaaacttt cgctgatcaa gttgacgaga tttaattgac
    32401 caacaatcgg aatatgaaga tcaatccaat acttcacttg ttccgacccc atcataccag
    32461 caaaaaagct catcgcaatc gtcactgaaa tcaagatgct atccttttgc accagattca
    32521 tttttggtat caaattacca accaatgtac ctaacaaaat cgcacacaac gaccccagag
    32581 cggtaacaat catcaaccat tgccaacgtt caccaaagtc tacttgatag ataaaacgaa
    32641 agacagctaa cataatataa atctctgtca aaacaatcgt aaagcttgct gccaaattag
    32701 cgattgaaac taacgattgc tttctaggaa tcaaggacaa gcgaatacca tttgacgatt
    32761 gatttgcttg ctgatcctgt acattgcgga ccccccacat cgtcccgtag agaatcgcca
    32821 ttccaatcag agtgaagaaa taaaaacttt tgaaactcat attacgacct gcatgaactt
    32881 cttgaaaaga tacttcttgt tcaaaaacag tcatgatggg cgcgagatca acgttctcca
    32941 cttctgccac tttactcgaa attataccaa tacgctgtaa aaattgatcc atgacgtttt
    33001 tcatcaacgt ctgactcatt cctgcttgac ccacatggag cgaaacagat gaaccatcaa
    33061 tgagataaaa accagccaaa tcatctgcat ttagcttttc attcgcttct acttccgata
    33121 gttcctgtac ttcaaataat ggtttatcat tgacttcgac ttgttctaag actgtggtaa
    33181 aattttccga aagcgctgtt tgatccgctt tgattacaat tccagtatta atcgtttcga
    33241 gattgccacc ttgatcaatc tcgccaaagg ccgccataaa catcaatcct aaaagaattg
    33301 gaaaggccaa tgtccaaaac aacatggatt tatttcgcag cagtattttt gtgcgatatt
    33361 tataaaaatg ccaaaacatt atttcccctc ctctagtctc tcaaatcctt gccagtaatc
    33421 tctaaaaaga catcattcaa ggacgcttct ttggttgcta tttttccgta actgatcttt
    33481 tcttcttgta agattgtaag aatcggcaac aatactgtat ttagttcagt agttgttact
    33541 tggagctgac cttttttata agcgattcca tcagaagcta ttgttcgctg acaacacgtt
    33601 ttctgggagt aatgggatgt cgatcgtcaa acgatccgcc gtccgtacca tatcttttag
    33661 agattctttg gttcctttag caatcacctg tccttgatcc atgatcacga tctggtcgca
    33721 aagaatttct acttcttcca tgtaatgtgt tgtatagaca atggttgctc cttgtcgatt
    33781 cagctccttg atactttcta aaattttatt ccgactttgg gggtctacag ctactgtcgg
    33841 ctcatctaaa aaaattaacc gcggcttgtg ggcaatccca catgcgatgt tcaaccgacg
    33901 tttcaagcca ccactaagtt ctcctggacg aaacgaaaca tatttttcta atcccactaa
    33961 ttgaatcacc tcttcaatca aggctttcct tttctctttt tcggaaacat ataaaccgca
    34021 aaaataatcg atattttctt gcacacttag ttcgtcaaag accgcaacgt cttgaggaac
    34081 gaccccaatc attgccttga cagcataatt ttcaggagtc attgttgtcc cgaacaattt
    34141 aatggttcct ttgtcataat ttaacaaaga aagcatacag ttgatggcag tcgatttccc
    34201 actgccgttt ggtcctaaca gacctaagac ttctccttct tgtacttgca gatcaaaatg
    34261 attcaatgct acaaaatctc catagcgttt gactaattta tcaatttcga taatcatcat
    34321 gaaaaactcc ttccaaattt cttgttgatc tgactataat aaagtgcaag aaaagaaact
    34381 agtgacgatt ggctaaacca cacatgacat ttgtcacaat gaaggagtct gctatgcata
    34441 aatctcggct actcaacgaa actagctttc tactggttat ctcattgctt ttgctggtaa
    34501 gtcaaggatt cgttgctatg caagttatct atttattgat cgccgtttta ttcactggtg
    34561 tcagttattt actgcctaca aagccaaatt ggattttcca agttattcta tttcttttat
    34621 gtgccttttt acctgatctt gtcctgtttc tgccagcgac gatgcgagtg atcgcccctg
    34681 aagaaagcca ccttgattac ttgtttttgt taggtttttt gttgatttcg acggttggta
    34741 atcatccccc catcaaggtc gcctttgtcg taattttgct gtttttttcc ttttacttac
    34801 gaaaaaaaga tcaacgaacg accgatcttg agaaaaagta cattgtctcc aaagatactc
    34861 attgggaaaa acaacaagag ttatcggaac aaaataaagc attggccctg tcacaagaaa
    34921 cacaattagc cttgcaaatt gtggaagaac ggaaccgaat tgcccgagac atccatgaca
    34981 atgtcggtca tctgctgtca agtgcgattt tacaaattgg tgcgcttgaa atgatcaatc
    35041 aagaaccaaa actaaaagaa ccattagaaa atttaagtgc caccgttcac actgggatgg
    35101 atcggattcg tcaaagtgtc catgacctcc atgagacatc gatctctttt caacactcct
    35161 tagagtttct gatcgctgat tttccttctt ctgttacgat tgaaggaaac ttgtttgaag
    35221 gattgaatga aacgcagcaa aactgcttct taatggtcat caaagaagcc ttaaccaaca
    35281 gtatcaaaca tagtcatgcc aaaaccgttg ttttacattt tcgaacgttg ccggcctttt
    35341 atcgcttgca aatcacgaat gatggcacca gcccacaaaa aaattctgct cccggtattg
    35401 gtttgaaaac gatgcgtcaa cgtgttcaag aacaaaaggg acaagtccat attttccaag
    35461 aaaaccagca atttcagtta acgatcattt taccaaggga gggacaccaa tgataacagt
    35521 agtcattatt gagatgatcc atttgtgacg aaatcgttac aaaccatttt agaaagcacg
    35581 aaagagatcc gtgtgttagg cattggtcat tgtgccaagg atgcgctagt cttatacgaa
    35641 acccatcgtc ccgatgtttt gctgacggac atccgcatgc ctaaacaaac gggcatcgat
    35701 gctgccaaag aaattttggc taaatttccc aaagccatcc ttttgctttt gacgacattc
    35761 aaagatgaag aatacatccg cgaagcattt tccatcggtg tcaaagggta tttaatcaaa
    35821 cagaacttgc aagcgattat tccttctgtc aaatccgctt ataatggaca ggccgtattt
    35881 ggtaatgaaa tcatcgagac tttcactcaa atgatgaaga ataaaccgac gattgatagt
    35941 catgcttatg cttcttttag tgaacgtgag cttgcaatca tcaaagaagt agctgccggg
    36001 aaaaacaata aagaaattgc agatgccttg tacttgagtg acggcactgt tcgcaattac
    36061 atcagccaat tactagaaaa gctagattta cgggatcgca cgcaattggc tatctatttt
    36121 tatcagcatt tgcaataagt cttatctaac acaaaaaaac ctgaatgatg ctccttcgca
    36181 tcattcaggt ttttttcaca tcttctattc aattgtcccg caaaccattt cctcacgatt
    36241 gaaagaatca aggattcgca gggctgccag gattcgttgg attgttcggc gtgttaggcg
    36301 gaaaaatagg cgccttgtct aacgtttttt ctagttttga ttcttcacga tcgtctcgtt
    36361 ctacatcagg actttcattc aatttgaatt ctttttccat ctcatctaac tttttaaagt
    36421 cattgccaga ttcttttgct aatttcgcta atttatcttc ggtttcttta gccataaagt
    36481 aacgcccccc ttttgatttc agcttagcat atcacactga aaaaacaaaa gaaaacgcta
    36541 accaacgatc atttttagct atttctcatc tttaaaatca agcatttttt gccggtataa
    36601 aatcgtaggg agaactgttt tccataccaa tcatcggatc tactaactgg ttatcgatca
    36661 cttctgctgt aagaacgaaa tctttagaaa cagcaggttc tacctcttct ttcttttctt
    36721 ctccaacaaa tgaagaaaac agatctggtt ccactgtttc cattacttgg tttttcgttt
    36781 cattcgtccc actgtcatct tctacaggta gcttttcttt tacaacccgt ggctcttttg
    36841 tatagttccc atcttcaaat ggatcatttc cgaccaaaga atcttcataa gctgctagtt
    36901 tggtccggag agtgatattc attttatcat tatccatgat ccttgatttc aacgtagtca
    36961 tccggcttgc tgattcattc gctacacaag tgacataggc ttgctcttcc cctaacaaaa
    37021 tcaacagttc cttactgcga gtcactgccg tgtagagcaa attacgctga agcatccgct
    37081 gatattgatg gaccatgggc aaaataacca tcttgaattc actgccttgt gatttatgga
    37141 ttgagcaaca ataagaaaga gtgattttat tccattcgtt tcttttataa gtgacctcat
    37201 ttgcatcaaa ctggataact aattcatcca ctttatcttc agaatctttt gccaagatga
    37261 tacccgtgat ttctcccata tctccattga atacattcaa ttcgggagta ttgactaatt
    37321 ggaggacttt gtctcctatc cgataaacgg tgtcgttcca atgtacttcc ttttttccag
    37381 gtttccctgg attgaatatc tcctgcatca ttttatttaa cgcatcgatc cctgcaggtc
    37441 cccgatacat tggtgctaat acttggatat cttgtgcacg gaatccctta tttttcgctt
    37501 tttccacaat tttagagatc aagggttcaa tttgataagc atcacttcgg aaataggaac
    37561 gatctttttg attctttgtg aaatcttttg gcagacgtcc ttctttgatc tcatgggcaa
    37621 gaggaataat actagaacca tcaccttgcc gataaatctg attgagttcc attttgggaa
    37681 tttgttcgat ttccaaaaga tcatggagta cttgaccagg accgacagag ggaagctgat
    37741 ctttgtctcc tactaagata acttgcatat tcgtcggaat cgctttgaat aatgtgttag
    37801 ccagccatgt atcgaccata gacatctcat caacaatcaa aagtccgcct tctaattctt
    37861 tcgtactgac tgaaggtgtt ttttcccgtc cattcagccc cagaagacga tggatcgtac
    37921 tggcaggcag acctgtcgtt tcgttcattc gtttagctgc tcggccagtt ggtgctgcaa
    37981 gtagaatagg aaaaatttct tgggtatatt ttgaaggatc caattccagt ccgttcaact
    38041 ctgcaaaaag attgacgatt ccgttgatca cagtagtttt ccccgtacct ggtcctcctg
    38101 ttaaaataaa tagaggggaa cggattgctt cagcgatagc ttgttcttgc gaaggtccat
    38161 actgtatgcc gaatattttt tcgatacgcc gaatattctt ctgcacttct ttttcaggat
    38221 aatggatttc ttttttacga ctgagaaggc gctggatcga attaccgatc ccccattcag
    38281 aaaagtacaa actgttttcg taaagattag tctcttcttg ttgtatcttt ccttcttcaa
    38341 caagttggat aattacagta gcaacctgat caggctggat ttcaattggg cgacttgttt
    38401 ctaaaatacg gatcgtttgc tgaagcaatg tctccgccgg tacgtatgta tctcctgttt
    38461 gaactgcatg ctgaaaaatc tgatgcaaga tagctgctcg aattcttctc ggtgaatctg
    38521 ctgcaatacc gatttgttcg gctagattat ctgcacgttt gaacccgatt ccttctatgt
    38581 cttcgactag ctggtacgga ttttcttcga tgatatccaa agactcgttc ttataggcct
    38641 ggtagatact gaaagctaac tgactaccga acccgtaacg attcagtccc atgatcactt
    38701 gatccatccc gtggtttagc cgaatcgtct cagcaagcat ttctcttttt ggcttcgtca
    38761 aaccggagat ttgttttaat accgaaggat cagcaatgat tttttcgata gcatcttctc
    38821 ccagcaattc tacaattttt tctgctgttt tcttcccgat tcctgggaat ttttcgctgg
    38881 aaagatacgc tacgatccca tttgcagttg ttggcttttc ttgctcatat ctttcgagtt
    38941 taagctgcct tccgtattta ggatgatcca ctaattctcc ataaaaacga taagtctcat
    39001 tttcttgaat atcgccgaaa cttccggtga ctacaatctc tttttcacgg taatcagctg
    39061 tggtttcact gatgtgtacc aaaagcactt tgtaaaaatt actaggattt tgaaaaaaaa
    39121 tcgcggcaac tgtaccaacg aaataaggca tttcttctgg ctgcatcgga agccgcctcc
    39181 ttcttctata gtaaaggttc tggtgctgtt tcagttccta aaaaatctgt ttggttccat
    39241 actttcagtt catatggcaa taatctgcaa ggctcttcag tctctaaaat cgtcaaacta
    39301 ctattgaaca atccacccat ttcacgcaac tcacttagct cttttcctgt catccattga
    39361 atggctgcag ttaaagaagc tccatgtcct acgaataaaa gtgggccatc tttatgttgg
    39421 gcaaccgcat ctgctacaac agtttcgata cgtgtgattg ccgattcaat cgtttctccg
    39481 tgaaaaggag aaggatcata cagatctaaa tgattacgca aattcggcag attatccgga
    39541 tatttctggt acatttcatc gatcgactga ccttctaaag tacccagccc tagttctctt
    39601 agcttatctg tataaaaaat ctctaccgga cgagcaagct ctttgttgat accttctgct
    39661 gtatcacgtg ctcgctttgc tgtactggaa taaattgctt taaaaggaac atattttacc
    39721 tttttcccaa atgctttgat ttcttcgtaa ctttgtggca aaagcggaga atcgccattg
    39781 ctcccttgaa aacgtctttc ctgattccat tgtgtcttcc catgtcgggt aaaatatagt
    39841 tccatacgca gcaaactcct gtctttcttt tattttactc tataataaac tgtctaatcc
    39901 agcttgtcat tttcatagtg atgcgtcaat tctaaattag ggaatccttg ctggcgaagt
    39961 gcttcataca ctatcattgc agctgtattc gataaattca atgagcggac atgctcatca
    40021 ttcataggga tgcgtaaaca cttttcttcg ttttcacgca taaatgtttc tggcaaaccg
    40081 gttgtttctt ttccaaataa aaagaagtgc tcttctccat cagcaaaatt ttcgtcacta
    40141 tatactttat gggcaaattt tgagatcaaa tgtagtttac gattacctaa aaatgttaaa
    40201 aaagcttcta aattactgtg gtaagtaata tttacatcat tccaataatc caaccctgcc
    40261 cgttttagat gtttgtcatc agttgaaaaa cctagtggct cgattaaatg aagcggagta
    40321 ttcgtggctg cacacgtacg ggcaatattt ccagtgtttg cagggattaa tggttcaaat
    40381 aaaacgatat gattcatcag gatcattcct ctcatattta tgttcgtttc tattttctga
    40441 caatttgatt tttgcataaa aaaaaacgta tcaactcggt gtcagtcact gcgagtcgtt
    40501 acgttagtga agctcttctc actaactttt atcagaacag gaacctgata aaaaccaatg
    40561 aaaaaacaac ttgttgctaa tataacaccc tcaacttttt tttgcaacct ctactcatta
    40621 aaaactgcta gaaaaacgtt ttctcttagt tccttttaat aattctatgt caattgtaat
    40681 cttcttcaaa ggattcttat ataagtctgc tttgattcgc tcttctacct gccattctaa
    40741 aggtgacatg gccaaaagat ctttttgatt ttcctctggt atctcaaatt cataggttac
    40801 acgctgacgt tcagttaatt tcattgcttc agcaaacttg gctactacac gttcattgga
    40861 ataactctgt ttttcaggtt gattcggata aaaagcctga cgtagttcct ttaaataatt
    40921 ttcttgtggc accaccttca acacttctcc atcttctgag agtacacgtt ggaattcttg
    40981 gtaatgagaa ggcgaaaaga tatttaaaac cgcgttgaat gtttgatcag caaacggcaa
    41041 attggtcaaa tcagctacac accaaaatgc ttcggcagat tgttctgtag ctgcgtagac
    41101 tccttctttt gaaatatcaa atccaacagc attttgaatc tttttcaatt ttgaaagttc
    41161 attcaagaag ctaccttctc cgcagccaac atctaatagc gagacttctt caggtaaatg
    41221 ctgcgcaata gtctcaataa cgggctgata catgccagat tggatcattc tacgccttgg
    41281 aacgaacatt ccttgatcgt attctgtttt aaccgcatgg gataaaaaat aaagtgttcc
    41341 ttttttggaa aggtcaaaac ggtgattatt tttgcatatc aagccattat cagacttttt
    41401 tacttcttct tggcaaaccg gacagcggaa cagctgatga ttttctgcca aaaactggcg
    41461 tcctcggtca atttttttta acatagttcc ccctcatttc tgttttatct taccgttaga
    41521 tgctcacttt ggcaactgct aaaaatatga tacaatgcct agaagaaagt gaaagataaa
    41581 ggagaagatt agatgatcaa agaattttgc gctgaaaact acacatctat tcctctagca
    41641 attgctagag gagccgatcg gatcgaattg tgtgacaact tagcggtcgg tggcacaacc
    41701 ccgagtactg gtgtgatcga agaaacgtta agttatgctg gggaaaaaga tatccccgta
    41761 atgacgatta ttcgtcctag agggggcgac ttcgtctata acgatatcga attgaaaatc
    41821 atgcatactg atttgattga agcaaagaaa ttagggacgg atggcgtagt atttggttgt
    41881 cttactcctt caggctggct agacgaagag gccttagaac tattgatcga aacagcagaa
    41941 gggttgcaga tcaccttcca tatggctttt gacagtattc ctgaagatcg gcaattcgaa
    42001 gcaatcgatt ggctggtaga acatggtgtt gaccgtatct tgactcatgg cggtcctgct
    42061 gacaaaacta ttgtagaaaa tttcccacgg cttaaagaat tgattgccta tacagatggc
    42121 cgcttaatca ttttgcctgg tggtggtgtc actgcagaga atgcagcaca tgtagctaaa
    42181 gaattgaacg taagcgaagt acacggaacc aaaatcgtgg atcttcagtt ataatcttat
    42241 aaacaaaaaa acatttgcaa caggcataat gcttattgca aatgtttttg tttttttaca
    42301 taaaatagcc cataaataaa gaagcaatat tgaaataaat cagtacactc gtcaaatcgc
    42361 tcaaagtcgt gataaaggga ccgcttgcta ctgctggatc aaatcctaga cgatccatta
    42421 gcatcgggat taaactacct gcaagattcg ccactgtgat tgcaaatagc atagccatcc
    42481 ctatgacaaa ccccaagata aaattatgtt tccaaaaacc tacgattaga aaaatcgtga
    42541 tccccgtaac agctcctgtg acaacgccgg tcaagacttc agccaaaaca agtcgaacaa
    42601 atccatgatc tttctcatca gaaatcgcaa gtcgacgaac agccacagct agcgactggg
    42661 ttccagcatt accggcagtt cctgtaatca gcgaaatgaa gactgctaaa atactcgctt
    42721 cacttaccaa gtcttcatag tgactgatta aagaagcggt cgacatacct aaaaacaaca
    42781 gagtgatcag ccaaggcaaa cgtcgagacg ctgcctttac tggattttca ttaatttctt
    42841 ccacattgac cccggcaagt cctgaataat cgctagcagc ttcatcatca ataacgtcga
    42901 tgatatcatc gactgtgacg attcctaaca aatgatcatc ataatcggta acaggaagag
    42961 ctaagaagtc ataatctcta aatgtttgtg ccacatcttc ttggtcgtcg cctacatgaa
    43021 ctgaaagcac tcgttcgctc atcagatcag aaatcattgc gtcgtcatca ttcacgatta
    43081 ggtcgcgcag agaaataacc cctactagct gaccttcttg attaacgaca tagatgtaat
    43141 agattgtttc agctacatct gcttcgttct tcagcacgta catcgcagag cgaacagttt
    43201 gattggctac gatcgaaaca aattcagttg tcatgatcgc gccagccgtt tcatcctcgt
    43261 aatggagaag ttccttgatt tcactggcat cgtctgccga catcaaactt aaatatttag
    43321 caatttgctt tttatccagc gtattaagca agtcaacagc attatctgta tacatctcag
    43381 aaagcatatc agctgcatag ctaggacgca tttcatctag gtattctttc atatgttcgt
    43441 tgtcttcttc aatcacatcg aacatatccg ctaattcttt aggcgacaga taggaataga
    43501 tcagctgcct gttctcttct gtaagagatt ggtagaattg cccctgctca tagatatgca
    43561 tctctaaaaa actgtttctg aatgcctgaa tattattttc ccttaaatct tgagtcaact
    43621 gggcaaatct ttcctctaat tcttgatttt catccaaacc aatctcacct cgccttattc
    43681 ttcaaaccgt ctcacaactg cctccatgtc tgcggctaaa ggctgcttga actccagcaa
    43741 ctcttctgta aatggatggt aaaaacgcag atcataacaa tgaagggctt gtctttttat
    43801 tcccagatcc attcgcccgc catacatatc atcaccgagc agggcacagc caattgcgga
    43861 aaaatgaact cttatttgat gtgtccttcc tgtatgcagc tggatatcaa ctaacgcgat
    43921 ttctgttagt cgcttctcta tccagtactc agtctgtgcc gaacgtcctt catcagagac
    43981 cattcgcttc aatagcgaag aataatctcg ggcgatcggc aaatcgatat gtccatgtgc
    44041 cgataaatct gaaagccttc cactgactaa ggcctgatat tttttcacaa acttcttttc
    44101 acgaagttgc atatctaatt ttgcgtgcgc aaatccatgc tttgcaaaaa gcatcaaacc
    44161 agatgtatcc cgatctagtc gcgtcaccac atggattact tggttttcgt aaccttgttt
    44221 tttataataa gcctttacac gattagccat tgtcccattt ggatggtatt gggcaggaat
    44281 agacgaaacg cctgctggtt tatttactac cagaacgtgt tcatcttcat acacgatatc
    44341 gatcggtgtt tcatccaaaa gtacggtttc atgttctcct tcagcaggaa tcacaatggt
    44401 taccttatca tccttagcta gagaaaacaa gacattttct acttgatcat taactttgat
    44461 ttgaccgcct tgaaatttta ttttcgctaa cagacccttt gagatccctt tttcttttaa
    44521 aaaatatttt acttgctgag aatgatcttt ctcataatcc catgtaaact gcatatcaat
    44581 cctcactaat aaaggcatct ttcacacgat gccaaaaatg catatgtcga taagaagcaa
    44641 aatggatccg ttcttctgct attcgataat aaattgagcg gatatttgct tttgcgacat
    44701 tcaattgatc aaccgttaca agataatctt ctgtattttc caattttatt tctagccatt
    44761 catcatgggc aatcacgatc ggagaaccca aagtacgaaa cactcgatta ttcagtgaag
    44821 caatctctgc caactgaaag gcattgatac taggatggat cacagcaccg ccgacacttt
    44881 tattatatgc tgtcgaacca gtaggcgtag aaatcgtcaa tccatctcct ctaaaacttt
    44941 caaacagctc gtttttgatg tatacatcgg ctaccatcgt acgattggcc cgtttaatcg
    45001 ttgattcatt cagtgctaaa aaatgctggt ccggtgtttc atccaaataa ctgatccgaa
    45061 catccagcaa gggataacta acactttgtt cccggttcgt tcgtaagctg tctactagtt
    45121 cttccagctc gtaatcccgc caatccgtgt aaaaccctaa atgacctgta tgcacgccca
    45181 agaaacgcac atcattcaga caatgactgt agtgatggaa cgccgacaat aatgtgccgt
    45241 ctccgccgac tgagatgacc aattctggct catgatcatc tcgttgaatc cctgcttgtt
    45301 ctaaaagtgc gttcaaacgt tctgtcactt ctatagtctt tgcttcttta ttatgaacga
    45361 tcgctacttt catcccctgc ctcctctctt tcgctttctt tcttcaaaag cgttcgatag
    45421 tagacatcat ctgattttcc tcttccatgg gagaataacc gctgtgcttc ttggatttct
    45481 tcacggatac tggacatctc ttcatccaac tgataggcag cttctgccgc acgctgaagt
    45541 cgttcactca tttcttctgg aaaaacgcct tgatatttat aattcagcga atgctcaatc
    45601 gttgcccaga aattcatcgc aagagtccga atctggattt ccgccaaaat tttcttctca
    45661 ccgctgatca gctgtacagg atactcgatc accagatgat aagaacggta gccacttgct
    45721 tttttatttg tgatgtaatc tcgttcttgg agaactttca tgtcttttct tttacggatg
    45781 atctcaacaa cttgatggat atcttctaca aactgacaca tgatccttaa cccggcaata
    45841 tcagacattt cttcttctaa acgtcccatt ggaatatgcc gtaattgtga ttttgttaca
    45901 atactatcga ctggcttgac tctcccagtg acaaattcaa tcgggacatg tcgattttgt
    45961 tcacggaact gtttgcggat cccccgcagt ttcactttca attctgatac agtttgttca
    46021 tatggtgcta aaaatgcttc ccaatctcgt tccatagctg atacctctac ttcattaaca
    46081 ttcattgttt attctaccat atatgcaaag ttttttcgta ctcttgattt atgcattgac
    46141 caaaaaaaga aaattaggca aaatagacaa aaaggagtcg atcaaatgac acaaatgctt
    46201 gaaatcgaat ataaatctat gctgacaaag caagagtacg agatattcat cgctcactat
    46261 caattaacag ataaagattt ccgtgtacag actaatattt atttcgatac cgttgatgaa
    46321 cagctgaaga aacaaaattg tggcctacgg atccgtttcg ttggtcatca agctgaatat
    46381 acattaaaaa cacctgctga aaaagggcgt ctcgaaacaa cggatacctt tataacagaa
    46441 gaagcagaag catttatctt cgaaaaacgt ctgcctcgtt caggcgcagt ttttcaaaaa
    46501 ctttctgatt tgaatatcga tcctgcttca ttaatccaaa ccggaaagct tacaacaaaa
    46561 cgggctgaat tccctattga agaaggactt ttagctattg atgaaagctg gggagacaat
    46621 ctacacgatt ttgaactgga actagaggta ggggatgcat ctgtgggaaa aattgctttt
    46681 ataaattttt taaaacgttt ttctttacct tatcgtcctg ctaagaacaa gatccaacgt
    46741 atgcttgaag ctaaaaatta gaaacctttc attttcacaa ggcacttgag ttcatttttt
    46801 tacttttttc tgttaagtta taggatgtga aagaatctag tcactagttg tatagaatct
    46861 attttggggg agattcatat gatcgaaatc tatttatttg ttaatccttt aggggaatac
    46921 tgtttcgaaa tggaacaaca gttattacaa tttatcgaag aagagtacgg gacaccatca
    46981 aaagaaaaga tgcaatttcg ctttcttcct ttggtcaatt tgcagacaat tggggatgtc
    47041 atgcaacaaa aaggaatttc tcagaacaat cttgaggaac gtaatcactt attctcaatg
    47101 acttattctg cagcattgga ctgcaaagcg gcgcagtttc aaggaaagaa aaaaggccgc
    47161 cgttttttga tcaaattgca agaagcagtc ggctgtaatg acataccata ttctcaagaa
    47221 cttgctgaat ccatatttga agaaatcggc ggcgatttga atatgtttaa agaagatcgt
    47281 cagtctgact tcgttaaaga agtattcttt tctgatcaaa aaattgctcg ggaaatgggc
    47341 atcacaaaac acccttctgc agtcgtttac aattatgcct gcgaccgtga ttttggtgtt
    47401 ttagtagaag taaaaggtac aagtagcctt tggagaatca cagagctgtg taaaacaaca
    47461 gataactcat atcaagtttt taatggtgaa catttacata caaatgattc tcagttccat
    47521 tcaagaacta gccatttaca cttgctttct aattaaaaaa ata
//
LOCUS       DAFEPQ010000015        41209 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.015, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000015
VERSION     DAFEPQ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.015"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(26..328)
                     /locus_tag="LP543_000951"
     CDS             complement(26..328)
                     /locus_tag="LP543_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2321131.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="LP543_000952"
     CDS             complement(353..2446)
                     /locus_tag="LP543_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL2321132.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="LP543_000953"
     CDS             complement(2935..3699)
                     /locus_tag="LP543_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HBL2321133.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="LP543_000954"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="LP543_000954"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HBL2321134.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="LP543_000955"
     CDS             complement(4704..5780)
                     /locus_tag="LP543_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBL2321135.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="LP543_000956"
     CDS             complement(5804..6397)
                     /locus_tag="LP543_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HBL2321136.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="LP543_000957"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="LP543_000957"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HBL2321137.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="LP543_000958"
     CDS             complement(8067..8366)
                     /locus_tag="LP543_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HBL2321138.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="LP543_000959"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="LP543_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HBL2321139.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="LP543_000960"
     CDS             complement(10121..11032)
                     /locus_tag="LP543_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBL2321140.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="LP543_000961"
     CDS             11152..12009
                     /locus_tag="LP543_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL2321141.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="LP543_000962"
     CDS             complement(12196..12558)
                     /locus_tag="LP543_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBL2321142.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            12904..14343
                     /locus_tag="LP543_000963"
     CDS             12904..14343
                     /locus_tag="LP543_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2321143.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="LP543_000964"
     CDS             complement(14350..16902)
                     /locus_tag="LP543_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBL2321144.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..18347)
                     /locus_tag="LP543_000965"
     CDS             complement(17016..18347)
                     /locus_tag="LP543_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2321145.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18313..19791)
                     /locus_tag="LP543_000966"
     CDS             complement(18313..19791)
                     /locus_tag="LP543_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2321146.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="LP543_000967"
     CDS             complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="LP543_000967"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBL2321147.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22401..23828
                     /locus_tag="LP543_000968"
     CDS             22401..23828
                     /locus_tag="LP543_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2321148.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23920..24285)
                     /locus_tag="LP543_000969"
     CDS             complement(23920..24285)
                     /locus_tag="LP543_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBL2321149.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(24282..24647)
                     /locus_tag="LP543_000970"
     CDS             complement(24282..24647)
                     /locus_tag="LP543_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBL2321150.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24839..25087)
                     /locus_tag="LP543_000971"
     CDS             complement(24839..25087)
                     /locus_tag="LP543_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321151.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25086..25214
                     /locus_tag="LP543_000972"
     CDS             25086..25214
                     /locus_tag="LP543_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HBL2321152.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25271..26965
                     /locus_tag="LP543_000973"
     CDS             25271..26965
                     /locus_tag="LP543_000973"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HBL2321153.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            26969..27088
                     /locus_tag="LP543_000974"
                     /pseudo
     CDS             26969..27088
                     /locus_tag="LP543_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            27739..28872
                     /locus_tag="LP543_000975"
     CDS             27739..28872
                     /locus_tag="LP543_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBL2321154.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28903..29223
                     /locus_tag="LP543_000976"
     CDS             28903..29223
                     /locus_tag="LP543_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HBL2321155.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29252..29716)
                     /locus_tag="LP543_000977"
     CDS             complement(29252..29716)
                     /locus_tag="LP543_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2321156.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29847..31104)
                     /locus_tag="LP543_000978"
                     /pseudo
     CDS             complement(29847..31104)
                     /locus_tag="LP543_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(31133..31450)
                     /locus_tag="LP543_000979"
     CDS             complement(31133..31450)
                     /locus_tag="LP543_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL2321157.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(31476..31796)
                     /locus_tag="LP543_000980"
     CDS             complement(31476..31796)
                     /locus_tag="LP543_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2321158.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(32260..33675)
                     /locus_tag="LP543_000981"
     CDS             complement(32260..33675)
                     /locus_tag="LP543_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL2321159.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(33692..35008)
                     /locus_tag="LP543_000982"
     CDS             complement(33692..35008)
                     /locus_tag="LP543_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2321160.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(35158..35856)
                     /locus_tag="LP543_000983"
     CDS             complement(35158..35856)
                     /locus_tag="LP543_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBL2321161.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(36145..37101)
                     /locus_tag="LP543_000984"
     CDS             complement(36145..37101)
                     /locus_tag="LP543_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL2321162.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(37214..38047)
                     /gene="msrA"
                     /locus_tag="LP543_000985"
     CDS             complement(37214..38047)
                     /gene="msrA"
                     /locus_tag="LP543_000985"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL2321163.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(38222..39856)
                     /locus_tag="LP543_000986"
     CDS             complement(38222..39856)
                     /locus_tag="LP543_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321164.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(39843..41177)
                     /locus_tag="LP543_000987"
     CDS             complement(39843..41177)
                     /locus_tag="LP543_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321165.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        12505 a         8474 c         6959 g        13271 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    17221 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    17281 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    17341 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    17401 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    17461 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    17521 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    17581 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    17641 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    17701 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    17761 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    17821 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    17881 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    17941 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    18001 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    18061 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    18121 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    18181 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    18241 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    18301 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    18361 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    18421 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    18481 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    18541 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    18601 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    18661 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    18721 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    18781 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    18841 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    18901 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    18961 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    19021 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    19081 tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat
    19141 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    19201 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    19261 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    19321 taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt
    19381 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    19441 cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc
    19501 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    19561 attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga
    19621 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    19681 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    19741 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    19801 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    19861 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    19921 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    19981 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    20041 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    20101 ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga
    20161 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    20221 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    20281 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    20341 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    20401 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    20461 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    20521 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    20581 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    20641 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    20701 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    20761 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    20821 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    20881 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    20941 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    21001 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    21061 ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg
    21121 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    21181 gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    21241 ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    21301 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    21361 aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag
    21421 ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac
    21481 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    21541 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    21601 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    21661 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    21721 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    21781 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    21841 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    21901 taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    21961 tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga
    22021 attttttttt tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg
    22081 gatttttctg ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa
    22141 aatagataac ctaaaaaaag cgaacaaaca aataaaatga ctgtccttca ttcactaaac
    22201 atatttttat ttttttatat tgttaaaaat tcgcaattta ttttttaata gaattatctg
    22261 ttttccaaat tttataacat aaaaaacctt ttttaacggc atttatatgc tttttcagaa
    22321 attaactgat gatttcaaaa agaaaacatg tcataatatt tcccatattg tgcaaaaaaa
    22381 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
    22441 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
    22501 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
    22561 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
    22621 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
    22681 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
    22741 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
    22801 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
    22861 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
    22921 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
    22981 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
    23041 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
    23101 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
    23161 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
    23221 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
    23281 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
    23341 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
    23401 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
    23461 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
    23521 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
    23581 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
    23641 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
    23701 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
    23761 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
    23821 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
    23881 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
    23941 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
    24001 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
    24061 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
    24121 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
    24181 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
    24241 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
    24301 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
    24361 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
    24421 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
    24481 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
    24541 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
    24601 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
    24661 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
    24721 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
    24781 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
    24841 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
    24901 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
    24961 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
    25021 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
    25081 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
    25141 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
    25201 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
    25261 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
    25321 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
    25381 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
    25441 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
    25501 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
    25561 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
    25621 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
    25681 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
    25741 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
    25801 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
    25861 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
    25921 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
    25981 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
    26041 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
    26101 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
    26161 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
    26221 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
    26281 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
    26341 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
    26401 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctatcgt
    26461 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
    26521 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
    26581 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
    26641 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
    26701 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
    26761 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
    26821 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
    26881 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
    26941 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
    27001 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
    27061 gcagatgcaa gcttttgaac accgtttacc aagaggttgt gaaaaagctg ccctgcatca
    27121 agtaataaga acacccaagc taaaactgct tcttatgttt ttgacttggt tggacttatt
    27181 accgcagatg caagcttttg aacaccattt accaagaggt tatgaaaaag ctgccctgca
    27241 tcaagtaata agagcagcca agctaaaaca gcttcttatg tttttgactt ggttagactt
    27301 attaccgcag atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa aaggcgttta
    27361 gtttcgagta tgaagaaaaa ttttgtgaaa atggttcctc acgtttttgc ataaatttga
    27421 cttaataccg agaaactgat cccttctgtc ataacctctt tttatttatg tctgtttctt
    27481 tttgaaaaca actatttttt caacgtttac tctcttgcca atccaacatc atctaacgga
    27541 tacaactatt tcaatgatta ccattacact aaaaaatcgg aaacacacct ctccgaacac
    27601 aaatacataa gactatagaa tgtttcattt aagaaaaaaa gcgtttcatt atcaaaaagg
    27661 taagatctcc tttaaaatga aattatctgt agtactaata atgcagacaa ttttatgaat
    27721 ctgaaaggag catctccaat gaaagcagtc acttggcaag gaaaacagaa aatggaaatc
    27781 agaaacgtag atgatccaaa aatattagaa ccaacagatg caatcatccg tatcacagca
    27841 acagccattt gcggctccga tctgcatctt taccatcatg gtgaagcagt tttggaaaaa
    27901 gggtatgttg taggacacga accaatggga atcgttgaag aagtgggatc tcaagttcaa
    27961 acattgaaaa aagggcaacg tgtggttatc ccttttaata ttagttgtgg tcattgtcat
    28021 ttctgcctga atcacatgga aagccaatgt gatgaatcaa atccagatca gttatatggc
    28081 gggcttttcg gatttggcaa gttgaacggt aatcattggg gtggtcaagc ggaatatcta
    28141 agagttcctt atgcagactc tacttcattt gttgtaccag ataatgatct tcctgatgaa
    28201 aaagttttat ttctatcaga tattttgccc acagcttatt ggagcgttga aaacgcagga
    28261 gtaaaaaaag gcgatacagt tgtgattcta ggttctggtc cagtcggtct attcgctcaa
    28321 aaattcgctg ttatggctgg agcagagcgc gtaatcgcag tagatccagt acagcaccgt
    28381 ctagataaag cggctaatta caatggggtt gaaacttatt tcttagaaga cacggcacaa
    28441 gctggaacag aattgtatga actggctaaa ggtggcgcag atgtcattat cgattgtgta
    28501 ggaatggacg gattagagcc tgtcaaagaa aaagcgaaaa atttagtaag cttacaaagc
    28561 ggcacaatct ctccattaca gatggcatcg caagctgtca aaaaatttgg aacagtccaa
    28621 gtaactggtg tttatatgac tccagcttct tcttacccgc tgcaagaatt ttttatgcgg
    28681 gatatcgctg tcaagcatgg acaggcacca attatccatc taatgccaaa aatttacgca
    28741 atgattgcag aaggactatt cgatcctaca caaatcatta ctcattcgct gccactatca
    28801 gaagcagcac aagcatatga gatctttgac aaaaaagaag ataaaaatat aaaagttgtt
    28861 ttgaaacctt gaattcagta aaaaatacag gtgattttct ttatgcccaa aatcaaacga
    28921 aatacccatg agatcgaaat aggaaaagaa gaagatatcg aaatacgagg acgtcgcttc
    28981 cgcttctatt tccaaaatcg ttacacattg atctcactgg ctgtcgattt attaaccggg
    29041 attttctata tttttggaag ccttgcttcc ttaactaaaa tccccgatcg atatggcacc
    29101 tatttttatt tggctggtgc tattttcttg acgattcgtc ctattctcag aatcgtaagg
    29161 aacattttta tttacgacga aaaaaaagca atgaagagaa caagcaaaaa aaaagaggca
    29221 tgagaatttt tcaaatctca tgcctctttt ttcaccctat cgtatcactt tgatacagac
    29281 gagcggtcgt cccactttct tccttgacca ttgtcaaaaa tgtccactcg tatcgttcat
    29341 aaaacgtggt atgatctgta attaagtaaa gtttttcgta cccaagttga tgcatttctt
    29401 ttctgatttc cgctaatatc cgcttggcta tcccctgctt acgcttttcc ggttcaacaa
    29461 acagggcaca aagatttggt gtcagatcag ggcggttatg gaagtcgttt tcaatgacgc
    29521 ccgctcctcc gacgatggtt tctttttcat ctaagaccac ataccattga ggaatacttt
    29581 ccttattagc gattccagta cgaatacttt cctcatacac aacagcagga actccccatt
    29641 ttgatgaaaa ccaattcgcg ctttgtttta ctaattctgg gtgttctctt aatttgatcc
    29701 agttatatgt aaccaatgtt taaacctccg actaaaataa aacgtttaca aaaattatac
    29761 aagtctttat gaaaattagc aatgacaaca atagtgatgt aaaaaaaaaa cggaaaacaa
    29821 tcacttgttc tccgtttttt gattaatcaa gcatctaaag cttcttttct tctttcttgg
    29881 atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa attgacagcg
    29941 gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat tggtggagtt
    30001 acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc aaagtaagca
    30061 acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg gttcaacacg
    30121 ataggtaacc caaacatcgt tggttcatta atattgaaga cacctggcgc aatcgacaag
    30181 tttgttacaa ccatatatgg tttgttttta cgtcctacta aataaatagc taacagtaaa
    30241 cctaaagttg cacctgttcc tcctaagttg acaaagctgt caaagaaagg tttgttcacg
    30301 ataaacggtg cgctatgccc agctgcgatt gcattaacgt ttgcttcaat ggctggcgca
    30361 ttgattgttt gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa gaaccataag
    30421 aatggtgtga taaatgcaag cagtaaggca gatggataag agtttgcaag tcccataaat
    30481 ggttcttgga ccactgtata gaaagaacct acgatatcat caacaccgaa tgcagcaaaa
    30541 attgcagcaa ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa tgaagaaaat
    30601 gcttttgcaa ctgccggtgg cacaccatca ggcattttga tgatcaattt ttcatttccg
    30661 cttaaacgta caaagatttc tgttgaaata atcccaacaa taatagcaat aaacaaaccg
    30721 tttgagctca tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga aatcacacct
    30781 gcggaaacac cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa gcaagaaagc
    30841 aatcaagaca gaaagaatgg caaatgtacc gttccaagca gcagccccga atcctttcca
    30901 tgattcacca ccaaaaattg agttcatcaa ttcttggaac ccgggaatcg gtaagttatt
    30961 gatcattact gctaatgctc ctaagatcat taacggcata ctcaccataa atgagtcccg
    31021 aatcgcaact aaatgccgtt gtgcaccaat ttttgatgct acaggtataa aatgtttctc
    31081 cataaatgca acaaatttat ccataaatct tccctctttt ctttactgta gattacgcgt
    31141 tcaatcgacg atacagatca atgatctctt ttgctaaatc tgtaaaagct atagatgtca
    31201 tcagatgatc ttgactgtgg accgtcaaaa gcgtgacttc aacgtgattt ccttgtgctt
    31261 cttgtgtcag catctctgtt tgagagtgat gtgcttggac taaagattcc tctgcatctt
    31321 tgattttttc atctgctaaa gtgaaatcac cttttttcgc agcagcaatc gcttccattg
    31381 catcgctttt tgcatttccg ccaaacatga tcagacccat gatggcttct agattttttt
    31441 gatcttccat ttattgtatc ctccgataat ttttattatt tgtttattag tttttctgct
    31501 tggtcgagta ctttttcacc attcatcatg ccatagtcag ccatattgat tacatctaaa
    31561 ggaatgcctt taggtgctag cttttcagca aattgatttt gcataaagcg tacttgcggt
    31621 cctaaaagta aaacgtctac atttttagat tctagttgat tatccgcatc tgaagcagat
    31681 acagcaaaga tgtctgcttc cattcctttt gcttctgctg ccttttgcat ttttgtgact
    31741 aggagacttg tactcattcc agctgaacat acaagcatga ttgttttctt ttccatttta
    31801 aacacgttcc tttctatact tacacttgta tataagcaag tagcgtgcca actttaaaag
    31861 cgctttaaaa agttgatatc atcacattta aggtctatac caagttgcat acacactaaa
    31921 aaagaaagcg cacactaaac acacaaatta atttttatta ccttttttct tttaacgtgc
    31981 acaaaaaaag ttgtgacaca aaacttgtca caactttttt caaataaacg gtattcaaaa
    32041 gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca taagatgttg tttttacttt
    32101 gctattctta ttacttgatg caggacagct ttttcacaac ctctcaataa acggtgttca
    32161 atcagctgac cctgggtatt agctcaaaac gctggattca caacctcttt tatttcaaat
    32221 aaccagtaaa tacaatgaac atctacaaaa aacttctatt tatttataga tttcgtcagg
    32281 caaatccaat tctctcttat caatcaaagt ctgataccac gaagcagatt ttttcaatgt
    32341 tctgcttcgt tcatcgttca attcgattcg aactaatcca tatcgattct taaatgcatt
    32401 cattggtgac acacagtcag tgaatgccca taacatatag ccttcgcaat ttgcgccttc
    32461 ttctgttgct ttgatcaagt aatgcaaatg ctgtccgata aattcgatac ggtaatcatc
    32521 ttgcacgatt cctttctcat ccatatactg ttcttcatat tctcttccca taccgttttc
    32581 tgttatcagc caagggatat tatgataatt ttcttttaga tacatggcta tatcatacat
    32641 gattttcgga tagatttccc atcccctgga attattcatt ttcttcccag gtaaatcaaa
    32701 gacttcataa tatcgttcag gatggaaagg tgtctcttca ttccattgat aacgaggggc
    32761 gcgaacgcgt ttaggataat attgattgat tccaaggaaa tctactgtat gatgctgaat
    32821 cgtttctaaa tcctctgttg caggatcgaa ataaatcgat tgtttttgac acaactccag
    32881 gaactcttct gtgtagtatc cttttaccat cggatcaaaa aatactcgat tataaaacaa
    32941 atcatacata taagcagctt tttggtcttc tggagaattg gatcgagcat agaccatttc
    33001 aggatttaaa atacagccaa tccttccacc ataatttccg ctgcggtaaa tctctactgc
    33061 tttagccgtt gctaaaactt tatgataatt ccacatcatc cacttttttg tgttttgttc
    33121 atgcggccag cgaacagcat ccaaatagca acgcgtttgg ggaacgattg gttcgttgaa
    33181 tgtaaaccag ttttttaccc tatcaccgta acgatcaaag gcaatttgag catacttagc
    33241 ataaaaatca actacttttc ttgaagacca gccatcatat ttttccaaaa gaaaagctgg
    33301 gacttcataa tgttccaaac aaatcatcgg ttctacgtct gcttcgatca aagagtcgat
    33361 catttcactt atatgctgag catattcttc atctactata cagttctcat aatcagtaaa
    33421 aaaacgagcc caattgatag aagtccgata atgtgtcaga tggatggatt tcatcagatc
    33481 cacatcctgc tggtattgtt ccataaaatt agttgctacg gctgggccat acccattatg
    33541 ccaaacaaaa tgttcatttt tataccagct atctataaac gaatcctgtc cttctttttt
    33601 tcctttccaa ccttccgttt gccaagcaga agatgcagca ccaagaataa aattttttgg
    33661 aatagtaatt ttcatgattt cctcctgttt tttagatccc atcattttcc acacgatttg
    33721 ctacttttac aaatggcaga tagatcaaaa tagaaacaat aatacaaaca agttgcgtaa
    33781 taactgcacc gatatttcct gctgttgcca gataagcatt gacgatcggc ggcattggcc
    33841 aagggaccat tacaactgct ttcgccgcaa atcctaaagc ggttgcaaaa taaccaatcg
    33901 ttccagtgat aagaggcgtt aagataaacg gaatagctaa aattggattc agcatgatcg
    33961 gcataccaaa aataactggt tcattgatgt tgaagatccc aggagcaagg gataacttgg
    34021 tgatttgttt catatcttcc cgttttccaa ctaacaatac tgcaatcagt aaaccgatcg
    34081 tgactccaga cccgcccata ctcatataca tatcccaaaa tggcatagta ataatgttcg
    34141 gtacttcttt tccagcttca aaagcttccg tatttacagc aattgccgct aaaagcaacg
    34201 gttcccgtat cggcttcacc atttgatttc catggatacc aatcacccaa aagacttgcg
    34261 caataaacat caatagcaaa atacccggta acccttgtac gacaccttct aatgggcgtt
    34321 gtacgacatt gtaaatgata tcataaaggt acattcctgt aagttctttg atgataaatc
    34381 ccgcagccgc gatacccgat acagtcagaa tcgtcgggaa taaagcagaa aaactagcag
    34441 aaacattcgt tggcacggta tcaggcattc tcagctgtag tttcttctgt ttcctcaacc
    34501 agcaataaag ttcaatagaa aggatggaga ccaacattcc caaaaataac cctttcgtat
    34561 ctgtatattg tttagccaat acgttttcta ctataacagt ctgattttca ttcactaatt
    34621 cataaacagt tggattaaca gataaataag acatcactgc gatgatacca gggaatacac
    34681 catccacttt attcagcttg gctaattcga tccctatcag aaaaacagca tagatggtca
    34741 agaaacttag tgtgacatag ctgatggcag atgcgatagg ctttaattct tctaaaaaac
    34801 gcaagaaact tatctgtgct aagccatttt ccgagtcaaa gaccattgct gaaaacaatg
    34861 tgccaaaagc acccgtaata attaccggaa tcaatgcagc aaatgcgttt tttatcacca
    34921 tgatatacct taaattattg attctcgttg ccacgttccc taaaacattg gctaatttat
    34981 ccataaaact cattccagat cccctcattc ctgcttcttc tccttgacgt catctctttt
    35041 tgtctattag tcagcgctta catttcgatg aacagaaact atttactcct cttttttacc
    35101 aaatataaaa caaaaattca tctgatgtat ttatcataaa atacatcaga tgaactgtca
    35161 attagtttct ttttctccta ttattttctg taattctctc cgatttattg ccatatggac
    35221 agtcattgca tcaaacgcag caagtgcatt ccgcctttcg atggcctgaa cgatctctgt
    35281 atgaatatcg atcgtctcga ttttcagatg ctggatatca taatttttat taaacaaagc
    35341 aattgattca ttaatgattg ggataatatg cagcaaggct acgtttccgc tagcttctgc
    35401 aatcatcgta tgaaaatcga tgtctaattg aacatgttct tgtgtgcctt ctcgaaaaga
    35461 agtttcaatc ttttgcgtta attttttcat ttggacgatt tgattcttcg ttgcatattc
    35521 cgctgccaaa cctgccatct taggctccaa taaatatctc agttcaaata agtctcgtat
    35581 catccgatcc tgatcttcaa tcaaagaaaa accgagtggg tcattggcaa tccctcgctt
    35641 atcactgaca aatgtccccg ctccttgccg aacttcaagg atattcctgg aagctagagc
    35701 ccgcacagct tctcgcaccg tgcttcgccc cacctctaaa taatcggaca attcatattc
    35761 attcggcagt ttatctccta cttgcaactg catttccttt aaatatctaa ggatcttgtc
    35821 agtagttact tctactaggg ttttattttc ttccaaacca catacctcct tttcatctga
    35881 tgtatttttt cgtaattatt ataacacgtt tgaatcatct gatgattttt tgtatacaaa
    35941 attgattaat ccgttcatag acatacaaac gaagaagata tttaccataa aaaagactag
    36001 gataaaattc actcctagcc ttcgatcatt tttattttga acgacaaaaa atatgatgtt
    36061 caataacaga cgatcttcat tcgattcttg atgtcatctt ttgttttttt taatatatgg
    36121 ataaaaagtt tgattacatc gtttctattc tagaatttgg tcgattctca attcttcttc
    36181 tggtgagaaa tctaaattat ccatcattcg aacactttct tgtaactgac ttaaccgact
    36241 tgctccgacc agtacacttg taacagattt ttgtcgcaaa ttccatgcaa gggccatttg
    36301 cgctaatgat tgtcctcgtg atacggcgat tgtttgcagt gctttgattt tttccagagt
    36361 tgatccaact tgttcttctg aaaggaatgg tatttcctta cgatgtgctc tagaatcttc
    36421 aggaatacca tgtagataac gatttgtcag caacccttga gcaagcggac tgaacgtgat
    36481 tgcccctagc ccttcttctt ctaatactgg agatagatcg tcttctagta gtgcacgaga
    36541 aaacatattg tagcgcatct gatgaataat gaacggtgct tcctttcttt ttaaaatttc
    36601 agtcattttt tttgtatctt ctccattata gttggatata ccaatatata atgcttttcc
    36661 ttgtcttaca agttgcatca aagcttctgc tgtttcttcc aaaggtgtat cagggtctgg
    36721 gcgatgatga tagaagatgt caacataatc tagccccatt ctttggagac tttggtcaca
    36781 acttgaaatg atggatttac gagagcccca ttccccatat ggcccttccc acatatgata
    36841 acctgctttg ctagaaataa tcagttcatc acggtaggct tgaaaatctt ctctaaaaat
    36901 acgcccaaag tttttctctg cagctccagc agggcctcca taattattcg caaggtcgaa
    36961 atgggtaatt cccatgtcga atgcgcctct taaaatctct ctttgattat ggataggatc
    37021 ataatcacca aaattttgcc ataatccgag agataaaatc ggtaatttca aacctgaatg
    37081 accggattta cgatattcca ttttttcata acgtgcacta tctgcaatat acaatttttc
    37141 tcctcctcat caaatctttt taaaacgctt tcttttttct atttttagtt tactacattt
    37201 taaagaaaag gagttaatca aaatgatttt ttcgtatatt tttttgccat ttgtttttta
    37261 ctcgttgata gatcagaaat tgattacgtg cttgctggat ttttttataa cgtttcggtt
    37321 gttttttgta aaattgctga tgatagtttt ctgctggcca aaacgtaccg gctggttcga
    37381 tttttgtgac gattggttgg tggaaagttc ctgaactgtc tagtttctgt tttgattgtt
    37441 ccgctaattc tttttgtcgt tcgccttgat aaaaaatcac tggacgatat tgcttccctc
    37501 tatcttgaaa ttgtccaaat gtatctgtgg gatcagtaat ttgccaatag attgtcagca
    37561 actcgctata acttatttct cttgtgtcat aaatgatctc cacagcttcc acatgacctg
    37621 tgtaaccacc acttacttga tcataatttg gtttttcaac atgtccgccc gtgtatcctg
    37681 acaaaacaga taggatccct tttttcgatt caaacggctc gaccatacac cagaaacatc
    37741 cccctgcaaa tactgcacgt tcttgatagt cagcatcttg catggcatgc ttggaaaagt
    37801 catatttttg ctccttatca aatccttccg taatcaataa ataaaagtcc attacatcag
    37861 gcgtcagatt cattcggata gctaatggac gtagtgttgc ttctagtttt gataactgtt
    37921 ctttaacgga tatattttct cttatgtcat tctttgtctg aatcaatact tttcgttccc
    37981 aatctcgtgt attcggattc aaaatcagat tataaagtgt ttgaatgaat atttgttcct
    38041 catccataga ttctcctcct ttccgaagaa gacaagctag tgactttagc tgtaatttat
    38101 cataaagtga ggaaataagc gattacttca tccccctaca gatttttcgt ataaaaaaga
    38161 gagaatcctt atcgacagat tctccttttt aatcaattta ttttataaat gaaaagttaa
    38221 tttacctttt atggaaaact attttaaaaa ataaacataa atatgcagtt aatgtaacaa
    38281 gataagaaat aatagtcgtt actgtataac ttgtgcttac tttaatatca gcaactcctt
    38341 tgactccgct tatttgtacc aatccattag cattcacctg ctcctttaca ggttgatgat
    38401 ttatttctac attcgttcct aaataagcat atacaggaag tgtgattact tggctcttct
    38461 cattagttac tctatatatg atagaactac cagttgtttt ataaggaagg tttacccatt
    38521 gactgccaat ctttgcttcg tgttttatta tatgctcctt attctccaat gaagcgatag
    38581 gagaataatc atataaaaaa taatccattc ctttagtggt atacgcctct actgtttctt
    38641 tgtttacaac aatatgtccc ggctgagaaa ttattttttg attgaaattt tttttactag
    38701 aaacagttaa agtgattaat aacaaaacag tcataaataa ataaaatagt tctcttgtct
    38761 tattgtatct tctatttttg aaaaatatca tcgttaatat cagagaagag aatattattt
    38821 gcaagcttaa gatccgccaa ggaaattgta acaatttggc tggcgtattt tgcagaagat
    38881 accatggcac caaactagtt gtagctagcc aagtcaaaca cgtaccataa taaatctttt
    38941 tttctatgtt tgttaatttt ttaaataaaa tcaggggaat acataaagat aacagaccaa
    39001 ctagtcccat tgagtaagta gttaagtcgt tgttcatagc ttcagtcaga ctatcgaaca
    39061 cattagatgc atgagcatat aaatttgtca cgctaggttt gttgattttt tggtagagca
    39121 tttgttcaaa catcggatac caaaaagcac tcgtcagaac caatgtcatt cctgccgcac
    39181 taaataattt tttaaatata gcaataaaat ctgcattctt gaaaccttta gaaaccaaac
    39241 gaataaaaat aaaaatgata attaatgctg tactcattaa tgttgatagt acatggctgt
    39301 acactagcaa actcatactc aatgctagct ttacagacgg atacttttct tcttttccag
    39361 agatgatttg ataagcataa aataatacag ggataacaag tgttattgcg atagcttcgg
    39421 caattgcgga acgatagtag atgtctactg tccgataagc agagaaaata tagagacaac
    39481 taaataaaaa tgccgctttt ttttgtttta aaaaccgctc gccataatga taagctacga
    39541 gtgttgtaca caagttcaat aaccaaacgt aaataatata gcttacaatc aaattttttg
    39601 tcatccaata taacattaca gctggaaaaa aagtcagaaa gggataaaaa taatttacac
    39661 catatccagt ataattgaat gtctcataat taatcggact ggtaaaaata gtcgataacc
    39721 ccttgattcg agataagtga aaagaaagat ctccagtggt ataaatactc tgtgtatttt
    39781 tgaaaaagat gaaagaatat atactactaa ctataattaa tccactcaat attactactt
    39841 ttttatttat tttcattctt cttgtttatc tcctttttag tcacatatcg agaatctttc
    39901 aacttattag gtgcaaatcg tttaaaactt gaatacacca ctaaacaata agtcagaata
    39961 ctgatccata aagccatgag agcagttgga gtatattttg ttgtaatttc tatcttattt
    40021 tctccagcat ttacatccac attcactaag ccatgttttt tataagagtc atttacgact
    40081 ttaccgttta cttttacctg tgtacctaaa taagcatata ctggtactgt cactttttgc
    40141 tttttgttgc ttgccactgt gtaagtaatg ttattatctg cagctttata cttccctttt
    40201 tcccacgatt ctcctacttt tacttcatgt ttttctaagt gaggcctttc ttttatcgct
    40261 tctgttgggg cgtaatcgta taatcctccc atatctgatg ttgtataacg tgggagtgtt
    40321 tcttcattga taacaatatg accgtgtttt gattggataa cttttgcgta attttctttt
    40381 gctgctatag acgtgacgag tagaaacacc acagtagcgc caatgattaa ccatgaatat
    40441 tttttattca tagtcttttt cataaaaaca agcgttatga tcattgaaga aaaaaagatt
    40501 tggataccta agatacgcca aggaaactgg agcaagctta tcggtgtttt ttgaaacaaa
    40561 aaccatggaa ataaattagt tacgcatacc catgtagaaa ctacgccaac atagatgatt
    40621 ttttcttttt tctccatttc tttcagtatg aataaaggaa aaatcaacgc aaccaatcct
    40681 aataacccca tagtgtatgt tgtaatatcg ttatttaacg cttgaatgat actatctgaa
    40741 atagaaagtg cttctagctg caaatctgtt ttgaagggac tgtgtatttc ttgataattc
    40801 atctgctgca acataggata ccaaaaatag ctagttaatg cacaagtcaa aaaaacagcc
    40861 tttactaaat tcacgaccac aaattttata tcgtttttta tttgcttgct taaaaacaaa
    40921 cgaactataa tcatacaagc aatcaatgca ctacacaata gtgtagttaa gacatgagta
    40981 tacacaagta gacttatact gacagataaa gcgatgactt ttttttctct attgaatatc
    41041 aatccataag cataatataa aacagctggg ataatcgtaa gagcgattgc ttctgcaatt
    41101 gcggatcgat aatagatatc aactgttcga taagttgcaa aagtatataa acaactaaat
    41161 aaaaaagcag catctatctt tttaaaaaac ttttctccat aataaaaac
//
LOCUS       DAFEPQ010000016        40930 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.016, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000016
VERSION     DAFEPQ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.016"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(37..1188)
                     /locus_tag="LP543_000988"
     CDS             complement(37..1188)
                     /locus_tag="LP543_000988"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBL2321166.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1201..1638)
                     /locus_tag="LP543_000989"
     CDS             complement(1201..1638)
                     /locus_tag="LP543_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2321167.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1653..3419)
                     /locus_tag="LP543_000990"
     CDS             complement(1653..3419)
                     /locus_tag="LP543_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBL2321168.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3683..4360
                     /locus_tag="LP543_000991"
     CDS             3683..4360
                     /locus_tag="LP543_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2321169.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4363..5490
                     /locus_tag="LP543_000992"
     CDS             4363..5490
                     /locus_tag="LP543_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2321170.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5690..6130)
                     /locus_tag="LP543_000993"
     CDS             complement(5690..6130)
                     /locus_tag="LP543_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2321171.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6141..8174)
                     /locus_tag="LP543_000994"
     CDS             complement(6141..8174)
                     /locus_tag="LP543_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL2321172.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8176..9879)
                     /locus_tag="LP543_000995"
     CDS             complement(8176..9879)
                     /locus_tag="LP543_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2321173.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9973..10923)
                     /gene="manA"
                     /locus_tag="LP543_000996"
     CDS             complement(9973..10923)
                     /gene="manA"
                     /locus_tag="LP543_000996"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBL2321174.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10962..11987)
                     /locus_tag="LP543_000997"
     CDS             complement(10962..11987)
                     /locus_tag="LP543_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBL2321175.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12196..13065)
                     /locus_tag="LP543_000998"
     CDS             complement(12196..13065)
                     /locus_tag="LP543_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBL2321176.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13524..15416)
                     /locus_tag="LP543_000999"
     CDS             complement(13524..15416)
                     /locus_tag="LP543_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2321177.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAMVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15413..17140)
                     /locus_tag="LP543_001000"
     CDS             complement(15413..17140)
                     /locus_tag="LP543_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2321178.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17156..17806)
                     /locus_tag="LP543_001001"
     CDS             complement(17156..17806)
                     /locus_tag="LP543_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBL2321179.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18010..18306
                     /locus_tag="LP543_001002"
                     /pseudo
     CDS             18010..18306
                     /locus_tag="LP543_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            18478..19098
                     /locus_tag="LP543_001003"
     CDS             18478..19098
                     /locus_tag="LP543_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBL2321180.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19190..20356)
                     /locus_tag="LP543_001004"
     CDS             complement(19190..20356)
                     /locus_tag="LP543_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL2321181.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20368..20721)
                     /locus_tag="LP543_001005"
     CDS             complement(20368..20721)
                     /locus_tag="LP543_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBL2321182.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20849..21871
                     /locus_tag="LP543_001006"
     CDS             20849..21871
                     /locus_tag="LP543_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBL2321183.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21890..22051
                     /locus_tag="LP543_001007"
     CDS             21890..22051
                     /locus_tag="LP543_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321184.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22310..22744)
                     /locus_tag="LP543_001008"
     CDS             complement(22310..22744)
                     /locus_tag="LP543_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321185.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22854..24713)
                     /locus_tag="LP543_001009"
     CDS             complement(22854..24713)
                     /locus_tag="LP543_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL2321186.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24831..25991)
                     /locus_tag="LP543_001010"
     CDS             complement(24831..25991)
                     /locus_tag="LP543_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBL2321187.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26298..27722)
                     /gene="araA"
                     /locus_tag="LP543_001011"
     CDS             complement(26298..27722)
                     /gene="araA"
                     /locus_tag="LP543_001011"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBL2321188.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27738..28436)
                     /locus_tag="LP543_001012"
     CDS             complement(27738..28436)
                     /locus_tag="LP543_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBL2321189.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28393..30054)
                     /locus_tag="LP543_001013"
     CDS             complement(28393..30054)
                     /locus_tag="LP543_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBL2321190.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30272..31672
                     /locus_tag="LP543_001014"
     CDS             30272..31672
                     /locus_tag="LP543_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBL2321191.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31924..33003)
                     /locus_tag="LP543_001015"
     CDS             complement(31924..33003)
                     /locus_tag="LP543_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2321192.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33089..34048)
                     /locus_tag="LP543_001016"
     CDS             complement(33089..34048)
                     /locus_tag="LP543_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBL2321193.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34060..35571)
                     /locus_tag="LP543_001017"
     CDS             complement(34060..35571)
                     /locus_tag="LP543_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBL2321194.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35593..36156)
                     /locus_tag="LP543_001018"
     CDS             complement(35593..36156)
                     /locus_tag="LP543_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBL2321195.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36162..37025)
                     /locus_tag="LP543_001019"
     CDS             complement(36162..37025)
                     /locus_tag="LP543_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2321196.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37039..37935)
                     /locus_tag="LP543_001020"
     CDS             complement(37039..37935)
                     /locus_tag="LP543_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2321197.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38110..39399)
                     /locus_tag="LP543_001021"
     CDS             complement(38110..39399)
                     /locus_tag="LP543_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL2321198.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39371..40888)
                     /locus_tag="LP543_001022"
     CDS             complement(39371..40888)
                     /locus_tag="LP543_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBL2321199.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT        11793 a         8584 c         7089 g        13464 t
ORIGIN      
        1 aaaatttcgt cacattcccc tttttcgtat cctattttaa gcgacttgct tggcataacg
       61 gttcacgttt tgcttgatat tcccgattgt tttcggatca ttgattcctg tgacttcttg
      121 aatcaattgt tccaagtttt catggatctt catttcatgc aattgacggc tttgttcatc
      181 ttcaggatca tagtaattaa aaataatccc cattgttgcc gtcaaatgtg ggcaagtcaa
      241 gttatgttct tgtagttctc tcactggacg tgtaaatctt tcttgataac ctaacttacg
      301 taatggtgtc cgagcgacac gtgtgatcgc atcagagata tttttgtttt ggaagcgatg
      361 aatgattttt tcaatatacg catgatgctg ctctgcatca aacccccatt tggcaatcag
      421 caaacttcct gtttcctgca aaactgattt taattgtgcc acaactaacg catcttgcat
      481 tgcttcatca atggtctggt aacctaacaa ggcacctgta taagcgacag tcgcatgacc
      541 agtattgaca ctaaatagtt tgcgttcgat atatggttct agatcatcta catataaaac
      601 cccttttaat tggatttttt tattttttct ttggctatcg tcaatgaccc attctttgaa
      661 aggttccact tggacgaata aaggatcttc atggtgttgt aatgggacga ttcgatcgac
      721 tgctgcattt ggaaagccga tatatttgtc agtaaatgat gtttccggaa gatatttata
      781 cacttctttt tctagaaatg ctgaaccgcc aatcatattt tcacaggcaa tgatatctaa
      841 agatgttgtg cttccttcct tttctctttc ttggatacct tttgcaatca actcagcaat
      901 aaaaggtaag atattcgggc caatggcggt agtaactaaa tctgcttcag cgatttcttg
      961 gattactttt tctggatttg tttgattgtt gattcctttg acatttttta cagtgatttt
     1021 ttcttttgat tcatctgcta attcgatttc gtattctttt ctttcattta aagcatcaat
     1081 gatcgtgtga ttcacatcta cgaaagtaat ctcaaatcca ttagctgcca aaacctctcc
     1141 aataaatcct cgaccgatat ttcctgcacc aaaatgtact gctctcattt ttctctctcc
     1201 ttaagcaatt gctagatttt ccgttacttc ttctttgctg agcgcgtctg ctagctggac
     1261 aacattgtcc atatcactgc aataaagtgc gatttgctgt acaagttgca aatgctcatc
     1321 tccgactcct gcaataccga acaaaacagt cgcaatcttt tcttcttctt cggtaccaaa
     1381 atctacacca tctggtactt gaacgacaca gatgccggat tttttcacat atttttttgc
     1441 ttcatctgtt ccatgaggaa tcgcgataaa attccccata taaacagata acattttgtc
     1501 tctatctacc atggcatcga tatattcctc atcaacgcac cctgcttcta caagcatctg
     1561 accgcaatag cggatcgctt cttctttggt tttaaaagat cgattgagct cgatcatgtc
     1621 gaatggcaaa ctttgcatgc tgttttcctc ccttaagctg tctgtttcaa atattgtgca
     1681 atcgattcgt atttttgcgg attgaccatg ctgttgactg ataaatgaat ggattgaggt
     1741 gcagccatct gtactttttc cgctaaactt tctttggcta cgatcagtga atcaggttca
     1801 tcttttaaat cttggatatt gacttttctt aacgggatag ttaaatggtt cttgtctaat
     1861 atatctttga gcacggtcat ccccattgtt gctgatccgg aacgatcatc gtggacaagt
     1921 agaatctctt tgatttgttt gatgtttgct gaaagaaact cttctgctgg ctcattctca
     1981 atttcagaaa ctgatgtttt ctgcttgctg cttaattctt gaatgatttc atcatatcga
     2041 ggtgaattca aaaagttttc tactgagaca aaacgcgcgc tcggtgcttt ttgttttgct
     2101 ctttcttgta gttcgacctg agtaacgatc aatgtattcg agtcatctgt cagttggctg
     2161 attgcttgat tggtcaccgt ttgaggcaag tctgcttcct gtactttttt tctcaagatc
     2221 gaagcgccca tcgcacttga tcccattcct gcatcacaag caaaaatgat tttttgtacg
     2281 ccagagaatg tagctccttg aggaatacct tgggcacctt tggaagcttg cttagctgcc
     2341 tttgtctctt gaacagattt ttcgaagtct tcatttgtat ctttcgcatc cgctttcaaa
     2401 attactgcag aaataccaaa cgaaacagct gcggcaacaa tcacgcccaa aataacagga
     2461 aggtaagaat tgaacggtgt catcgctaga atcgcaatga tcgaccctgg agaagctggc
     2521 gctcttagtc cagcatccat caattggaaa atgaaacttc ctgacattcc tccgcctata
     2581 actgataaga ataataacgg tttcatcata acgtaaggaa aatagatttc atggatcccg
     2641 cctaaaaagt gaatgatcat ggctcctggt gctgaagatt ttgctgctcc tttgccaaac
     2701 aaagtaaaag caagtaatac gccaagtcct ggaccaggat ttgcttcaag caagaataga
     2761 atagattttc cagcctctgc tacttgttca gttcccaatg gtgtcaaaat cccatgattg
     2821 attgcattat tcaaaaataa gatttttgct ggttcaataa aaatacttgt caatggaatc
     2881 aaatgggcag ataaaatgat ctctacacct tttgccattg cttgagtcaa tgcatcgaca
     2941 actggaccaa tcgcagaaaa acctaataac gctaatccaa aaccgataag acctgctgaa
     3001 aaattattga ctagcatttc aaaaccggat ttgatccgtt tttggaatag ctgatcaaat
     3061 ttcttgatgg catatccacc aagaggtccc atgatcatcg cgcctagcat cattggaata
     3121 tctgttccga caatcacacc catcgttgca attgccccaa caactgatcc gcggtcacca
     3181 gcaattactt ttccgccagt ataaccgatc aataaaggaa tcagataagt tagcattggg
     3241 cttaccatgg tagccaaagc ctcatttggt aaataaccat ctgggataaa tagagccgtt
     3301 aatacacccc aggcaatcag tgctccaata tttggcatga ccatgcttga taaagtactg
     3361 cctaattttt gtaccctcgc ttttagcgaa atgttgtttt tgggaatcgt ccgttccatt
     3421 ttgtctctcc ctctctcctt ttgatactct aattgtatcc gcttactaaa aagagaaata
     3481 gtcgtacttt ggcacgaaac tttgtgccaa aggtttgagt atgtcagcaa aataaaaaga
     3541 tttcggtatc agaaaacttc aattttaaat aaaactgtta ttttaatttt tttataatat
     3601 gcaaacaata acttttgtta gcatttaatt tagatgtttt ccattctatt atgttattaa
     3661 attttatatt ggaggcctat ctatgacatt actgatcaaa aacatgaaca tgaaacgtgg
     3721 taaaaaacgt gtgattcgac aattgaatct tttagtagaa tcaggagaag tagctgcttt
     3781 gattgccccg aacggatttg gcaaaacaac tttgctgaat ggcattgccc aactgctgcc
     3841 aacaacttat gatcaattgt cccttcacaa catctctcct actgcttata cagattatcg
     3901 ccggtctttc ttttttctag aatcaacgaa tcagttatcc gactatctat cggctgttga
     3961 ttatttgact ttgatcaaat actattggca gagcaaagta gaatgccaaa ctgttttcca
     4021 acgattgaag atgactgagt accttacttt gccgattgga aaaatgtctc taggaatgaa
     4081 acaacatgtc ttgcttgctg cttacttgat cagtgattca tctgtcatgt tattcgatga
     4141 accgttgaac ggattagacc caggaagtat tgatatcttg aatagcctca tcagacaatg
     4201 gcagaaagag gggaagacga ttcttctttc ttcccacgct atcgcgaata tccaatcgat
     4261 ttgttctaaa gcatgttttc taaaaaatgg agaaatcgtc acagaatcct caaatatgga
     4321 agagatttta gctatttatc gtaaattata tgaggtgtaa ctatgtcttt acttacattt
     4381 gaacgcaatc tcttactaaa aaacaaagta aactttttat ttcctctatt gttggtcgtg
     4441 ctctttgcat tcccactttt ttttgatcat aaacttgctt atacagaatt cgatgaactg
     4501 aatcataact atgaagagat gcaacgtttg attgaaacgt tgaaagaaga tgagaatgag
     4561 aaagaatttg tggaaagttt agagaaaagc aacaaactga tcgaagcaat cttacatgca
     4621 aaaaacacag ggaatgtcca gcaaatggtc gaagcaactt atcattatga aaaagatata
     4681 ctggatcgac tgatttctgg tcaaagacaa ggaattccaa tcattgaaca acaaaaacgt
     4741 gtcgagttgc tccgttatat gaaagaacat cagatccaaa gatattctat ttttgacttg
     4801 ccagcgcatc tccctttagc aaattactac gaaaatattt tttcaggaat gatttctagt
     4861 tttttgattt tatgtataac cgccttgttt ctttcttcca tcatctctta tgaaaaaaga
     4921 aagcaggtaa tctctctagt caatctatta ccggatagta tggtaaagaa acatagcatc
     4981 cgttttacca tttattatgg tgcagctatg ctaagtctcg tgatgccatt tttgatcgtc
     5041 tctatactag taataatcaa aaatggtttg ggagacttcc gttatcctgt aggaacgatc
     5101 attggtcagg agatacgaat ccttccgatg tatgagtatc ttttccagag ctttctgttc
     5161 ctgttgttgt gggtgctctt tttaagtaca atcagttttc ttctatctgc tttatttgaa
     5221 cattctctag tcaatctttt aggtacactt ctttgtcttt tcttagcaga gtatcgcttg
     5281 ttttcttcga ttggatggat agaatcaatc agtcattact tgccaacttc ttatgttgat
     5341 tttcaaaatg tgatcatagg aggagatatt ttctcgccac tagcttcaga acaggttact
     5401 ttcatgaatg gtatcttgac acttggtata tggagtatcg ttttattatt cataggtatg
     5461 ggcactatct atataaaaaa gagctattaa aacacaaaaa taataggggc ggtgacaaaa
     5521 accttgtcac cgtcccctat ttcaaataaa cggttttcta gaataaatag ttttcaaaag
     5581 cttgcatccg cagtaataag aacctcccaa caaaaatagc tcctcttatt tttatcttgg
     5641 ttgaagttat tacttgatgc aaggcagctt tttttactgt gaacattggt tatatatgtc
     5701 catgttcaaa aatcatttgg acttcttcaa tggttttcgc atcactgagc gccattactt
     5761 ggtcgatgtc tgaacagaaa aaggctaaat cttggagcac cgctaactga tgttcttttt
     5821 ttaaagccac tacaaataag agtgtcgcga tttttggttc ctcttttgtt ccaaagtcca
     5881 caccatcagg cacttggact aaatagattc cttcttctac tacttcttca tctttgtcag
     5941 tcccatgtgg caaggctacg aaattcccga tatagacact cgctttttct tgtctttcga
     6001 tcattgattg gatatatgcc ggtttgattc ccccttgttg ggtcatccac tggccgatct
     6061 cggtcaacgc ttctttccaa ttttgatagg attgatcgag caatattttt tctgctggta
     6121 gctgtatcat cttttcacct tcatttcttt atttcttcaa tcaggatttt cgctaacaac
     6181 tgataaacga tttctttatt tcctgagcgg aagatctcgg tgttcaagtc attcatgatg
     6241 attgctccac tgatctttcc taaaagaatc ccgtcttttt cttggattgg cgaaggcgcc
     6301 agcataacta acatacggga caaatcgact ggctgtctat ccatcccttc aatggtcaac
     6361 ggatgtttca aattgaaaat acaaaagatc ggtttagaaa tcaatgagct ggatgtatgg
     6421 aataacccca tatttgtatg aggaataccg ataggtgctt gttcatatcg cttaagcagc
     6481 cggcgatgaa ccacctcttc atccgttact agtccttcag gaaaataagg aaataagctg
     6541 cgaatcgttt cggaaatcgt ctcaggatta tccagttctt ggataaaaaa acgaccgagt
     6601 aattgattga tttgatccat gaaatgcatt gtttcctcat aagtatcttc tatcatccac
     6661 tcgttattgc tgttatacgt aattttttgc ctttttttct tctcaatatg cgaaaaagct
     6721 tcttttagct gcttgacttc atcttctagc agtagcgggg agactaaaat atattttcca
     6781 ccgtatcctg gtaaaataga tgtagaaata acaacatcat attcttcttg taaatcgatt
     6841 ttttttaaat cagcaatccg atagaaatga atttgttgga tgaagggaaa ataccttttc
     6901 aatcgtagtt ccaacatgct agtagaagct aatccacttg gcgaaaatcc tgcaatactg
     6961 atattgcttt ctttcggact tcgttccaat gaattcgcaa aatgaaggac catataagcg
     7021 atctcttctt cggataattt tttttcttga aaaatccgct ctactgcttt acggattgca
     7081 ttcgcaactt cctcgtattg aaccatgatt ttttctaaaa tcggattggt cagtgtctct
     7141 tcttgtaaaa cggctcttga aaacacacca gataaatgag tcaacagcat tttgtacaaa
     7201 gtttcatctt cataaaaatg gacattcgtc tcttcactta cttcttgaat cagttgtttt
     7261 actaaataag caagctgtgt atcgaacgtc tcttcaaaga aattctgatc aaatgagttc
     7321 gtgaagtcat ttaataatcc tgcaaaaaag acgatctcat ttactggata aagttgtttt
     7381 gtctcagaag cgatccgtga aaacaatcgt ttagctgtct gtagtaattc tttgtttgcc
     7441 agactattat atgtctcatt tgtcagtaaa tgatttttag tcacacgctc catcgtcaat
     7501 gtcagcatca aaaccaaatg ttcaagcttt ctatcactta gattggtctt tttttggatc
     7561 aattcttccc cgattacttt gcgtgcaaga gatagatgtt gcggatcaac gaatcgaaaa
     7621 aaataattca gtgatgcatc gaaagaaata ctatgaaaaa gttcatattc attgatttct
     7681 aattctaata cattcgctgt cagaagccgg cggtattttt ccggtccttc gatctcgtat
     7741 ccctggttcc gaatgatttc caaaggcagt tggcgaatac tttcttccaa ctgtttgata
     7801 tctgcataaa aagtggtatt actgatgcga tatgattcca tgaaatgagc aagactcaac
     7861 ggctctgtcg tcagtaacaa ctcggctaaa atcgcgtgtt gtcgtttagc agtggataag
     7921 tcattacttt tcccatctgt attttctttt aattgctcta atacttcttc tgatgcctca
     7981 atgacaaatg agcctctagc tgcttttttc aaagaagcat ttaccgattt caatgagact
     8041 tcaagattat ctaattcgcg gtaaatagtc cgtttactga ccttcaataa attcatcatt
     8101 ttttggatag atacaggtga cgggctactg agaaattctg ctaataattg tttttctctg
     8161 ggcgataaat acatcttata tttctccctt cacgagtttt tcgtattcat tcgtagctac
     8221 ccaatgttca actagaacta gccgagggat cttttcttct agctgttcag ttacagctag
     8281 ttctctttgt gcgatataaa tcgtttcttc ttggattttg attttttcta ttgattcttt
     8341 tgagataaca aactgttttt tcgctttttt tgctaattga cttaaaatcg tcatgcccat
     8401 agtttgtgag ccgcgtctgt tgtttttata caaaaataca atattagcag gcgacttttt
     8461 ttgttctgtg atatctgcta tctctttttt ttcttcccgc tttggaaatt ggatcgcttc
     8521 ttttattcgt gacagcaaga tatctgtttc caaaaagtga tccagtacat agatttgact
     8581 ttgaggtact ttttctcttg caagacccgc tagttcatgt tgcacgataa ccagctgatt
     8641 tacttggtta ttcaattcat agatagagct gtatccgaca gaaagatcga ttttttctgc
     8701 tgctaatttc tttttcagca gggcggctcc catagcactt gatcccatac ctgagtcgca
     8761 agcaaaaatg acggaatgaa tgatagatgg ttccaagatt tctccccttt ctttgaccgt
     8821 tttgttctct ttttctttat ttggtaattt caatagtatc gctgcacaaa tgaaggtcac
     8881 aaatgtactg attgctatcc ctatacttac cccagctagt tgtggataag gcgcattggc
     8941 aaaaatcacg agtagtgacc caggcgatac aggtgcagct aatcccgtat ctaaccattg
     9001 aaatatcaac gtgccgcttg ccccaccagc cattacagct aaaaatagat acggtcgcag
     9061 caagacaaat ggaaaataga tttcgtgaat cccgccaatc gcatggatca tcaatgctcc
     9121 actcgcattt actttttcct gtttttctgc aaaacaaagg atagaaagta agatccctaa
     9181 accaggtcca ggatttgcct ccatcaaaaa taaggaagag tacccgactg tttgtgagaa
     9241 ttcgattcct aaaggtgtca atacaccatg attgattgca ttattgaaaa atagtacttt
     9301 tagaggttct aagatcagac tgacaagtgg taacagattc attgtgacta gccaagacac
     9361 aacggaggcg agcaacaagt gaacactatt taaaactggt tcaataaaaa cgattccaat
     9421 tatacaaata acgctaccag tcagtcctac tagtaggttg ctgacaagca tttcatatcc
     9481 agccttgaat ttcacgtgga atctagaaac aaattgttca tatacgatgc cggcaagcgg
     9541 cccgataatc atcgctccta cgatttgtgg agcattggaa cgagcgatca accctaaaga
     9601 agcaattgca ccggcgactg cccctttttg tccccctacc gatttcccgc ctgaataact
     9661 gatcaagatg ggtaaaagca gctggatcat ccaatggcat acctcttcta atgtggtgtg
     9721 caaatcgcca gacacacctt ttgttgctaa tgacaagatt ccccaagcga taaacagact
     9781 tagatttggc atgatcattt catttaagtg atgtccagct tttctcagtt gtttcataga
     9841 cacgcgactg ctgttgtttg ctttccattt gtattccatc tttttcacca tacttctatt
     9901 tattgatgga cggattttat gtagaaaaag cagcaaacga aaaaccgtct gctgctttca
     9961 gctatctggg aactatttgc ctggttcagt cgcaatgatt gctagttcac cttggaccgc
    10021 ccactcagaa acgccatttg gaataataca gcttgttcct ttttctaatg gatatgtttt
    10081 tccgtcaacg acaaggtctc ctgctccttc gattaccgtc atcaatgtat aaggggcttg
    10141 ttttttgaaa gatgtgattc ctttaatatc ccactcatat acattgaaaa actcagtttc
    10201 taaataagtg acgatcgatg aattcccttt tcggacttct ttgatttgta attctggtgt
    10261 tttcgcaggg acagttgtta catcgatcga ttgttggata tgcagctctc ttgtttgtcc
    10321 ttgcgcatct tttctgtcgt agtcatacac gcggtaagtc gtgtcagaac tttgctgtgt
    10381 ttctaaaatc atgattcctt taccaatagc atggatcgtt ccgcttggta cataaaagaa
    10441 atcacctttt ttaactggta ctttcttcaa aaggtcatcc cagcgcccat ctttgatcat
    10501 ctcagctagt tcttctcttg tctttgcatg atgaccatag atgatttctg caccaggttc
    10561 agcatcgatg atataccagc attcagtttt tcctaattca ccttcatgtt tcatcccata
    10621 ggcatcatct ggatgtactt gtacagatag atcatcttct gcgtctaaga tcttgatcaa
    10681 taatggaaac actggttctg aaggatggcc aaacaattcc ttgtgttctg accatagttc
    10741 atctaatctt ttccctttga attcgccatt ttctactaca ctgacaccat gaggatgcgc
    10801 actgatcgcc caatcttctc cgattttatc gttagggata tcaaaaccaa agactgaacg
    10861 taaacgactt cctccccaaa ttttttcttg aaaaactggt tttaaaaaca taggttcctg
    10921 cataattcaa actcctctca tttttcatag ctattatagc attatttcgg taataccgct
    10981 ttctctttca aaaagttttc aacaatttca tctcgacata tgagatactc cgcacgatga
    11041 tcgacaggat gtacgcgatt tgacaaaaat atcaacgctt cttgtgtttt tatatcaatc
    11101 aagaaaaacg tacctgtata accagtatga aaaagaatcg catgaccgtc ttctacatcc
    11161 ttttttaagt cccatcctaa agaacgatag ccattttttt tcggtgtttg atcttgcagt
    11221 aaagaatcaa tcgtctcttc ttctaaaaaa cgaccattac ggatttttcc cttgttcaaa
    11281 tacatttgga caaaagtaat gctgtctttc aaatcaataa agagacccgc gttacctgca
    11341 tgttcagcca aaactcttgc tttcggatca tgagtttgtc cacgcaaaac ctcacccgaa
    11401 ggcagttgtt ctgtgggaac gatttttttg gaaggaaaag gaagaaataa gctgttcatc
    11461 attcctaatg gttccaatac ttgttcttga aagatttcca ctgcagtttc ttggtaaatt
    11521 tcctctagca tcaacccaag aagaatcgta ccggcatctg tatattttac ttcttttcct
    11581 agttgctcac ctggttctag cttcaaataa gcggcgatca attcttcctg agatagctta
    11641 tctctgtttg gtatccatgt tttgatatca gaagtatgtg tcagtaaatg acgcaaagtg
    11701 attgtctgat tttcaaattc aggaagatat ttatgaagcg gttgatcgag ttctatcttt
    11761 ccatcttcta atagacgtaa aactaatgaa gtggttgcaa cgactttcgt cagtgaggct
    11821 gcatcaaaca acagatcttc tgtcatcagc tgcttttcag gaacgacttg ggcaaagccg
    11881 gctgtaaatg tttctacttg attttctttt atgaaggcga ctgctacacc tggataaaat
    11941 ccttcttcca tctttttcaa aataagctgt ttcgtgtgtg gatacatctt tttctcctgc
    12001 ttttctatca gattcttaca gtccaagctt aaagcttaat ctatgaggaa tcaacctttt
    12061 ttacacaaaa aaactgcgcg aaaaaccgcg cagtttcccc cggtatacta ttttctcttt
    12121 ctatctctag aataagcgat agtatgaccg ttcataaaat gacgagacat gcgacaatcg
    12181 ttaagaaaga aaaagttaag cgtcgcatcg aatgtcagtt ttattctttt cttttggctc
    12241 actgacgaac caccattcga ttcctatagc atcatagatt gtgagtactg tgtgcttttt
    12301 gtctaagaag aaatcaactg atagttgctg caagtgagca atcagtcctt ccatgctttc
    12361 agtatcgatc atgaatttca gaaaatcaag tccaatcact tcatctgtag gcaaatcaat
    12421 tgcgcctccg ctagcttctg tcaaaccaac atggaaatct ccttcattca atacatggat
    12481 gccctcttct tcattatgta ccttcaaacc aagtacatga ttaagaaatt ggcgttcttt
    12541 tgttaagtta gtaacattta aatggatttt ttcaaaataa gaacctgttg atagtttact
    12601 gaatttttct tcagattttc ttaataatgc ttcttgatcc attgtttctg gaatttctct
    12661 ttggtcgttc ttattctctt catggtaaat ttccaattca ttctcttctg ggtctacgag
    12721 caaaatcccc atacgtccat gatcttctaa agcttctttg attggataat tcattttacg
    12781 gatacgacaa aggatatctg ccatttcttc tgtgctaggg ataatcaatg aaaaacggtt
    12841 cagctttttg atcaccccca tgtgatcatt cgctctagga ctttcttcca gcagcaacat
    12901 ttcacttcca gcttcttctg ttcctaaaat ggctaattcg ttttcttctc ttttcaaatc
    12961 aaagccaaca acgttcttgt aaaaatcaat cattttatct cggtctttca cccgaatggc
    13021 tacacttttt aaataggtgg cagcccccaa ttgaaaatct ttcatatttc ttgctcccct
    13081 ctacgtcaag ctttatttta acgatggatt cttcttcttt caagcaataa ctttgatttt
    13141 gttaaaaaaa atgtgagaaa attcgcattt tgcttgtttt ttgtaaatta atgagtaaaa
    13201 actcttataa aaagaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa
    13261 caaaaatagc tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
    13321 gaacaccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat tagagataca
    13381 aaacggaaaa atagcttctc atattttgcc gttttttgag ctaataccga aggacagctg
    13441 attgaacacc gtttattcgg aaaaagggac tgtgacacga cttttgtcac agcccctaaa
    13501 aaaaataata aatttgtact attctatgaa gcttcttctt cagaaaactg actgttataa
    13561 agatctgcat agaatccgtc tttttccatc aatgtatcgt ggtttcctgt ttctacgatc
    13621 gaaccatgat tcatcacaat aatgttgtct gcatcgcgta ttgtagacaa gcggtgagca
    13681 acaacgaaac ttgtccggtt ttccaatagg cggttcatcg ctttttgaat cagtatttct
    13741 gttcgtgtat ctacactaga tgttgcttca tcaaggatca ggacatctgg atttgctaag
    13801 aatgcccgcg caattgtgat tagctgacgc tgcccttgcg aaatattgct tgcatcctcg
    13861 tttaatacag tgttatatcc ttctggcaat ttacgaacaa aatcgtctac gtgagcagct
    13921 ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt tcccatattt gatattgtca
    13981 tagatacttc ctgtaaacag ccaagtatct tgcagtacca ttgaaaactg tgcgcgcaat
    14041 tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac gaatactacc tccagaaaca
    14101 tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc ctgcaccagt cggacctacc
    14161 atagcgacca tctcaccagg tttgacatcc aagttaaaat cagtcattag caagttatct
    14221 ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt atggtgtatc tttttcttct
    14281 ggaaggccag atttttcatt ggacatctct tcttcatcca acacttcaaa tacccgttcg
    14341 gctgaagcaa ctgttgattg gatagtgttc atcaaacttg caagttgagt gattggttga
    14401 gagaactgtg ttgtatattg caagaacgct tgaacatccc ctaatgtcat gttcccattt
    14461 gcaactttta ctccgcctag tacagcgacg aacacatagc ctagattttt aacgaaattc
    14521 atcaacggca tgatgattgc cgaaataaat tgagctttcc aaccagcggc atacagattc
    14581 tgattttctt tttcaaatac ttcctggtct ttttcagtat gattgaatgt tttaacaatg
    14641 gtatgaccac catatgtttc ttctacttgg ttattcaata aacctaaact tttttgctga
    14701 tctgcgaaaa acacttgtga tttcggcgct acgatcatca cgatgatcaa gcttaaaggg
    14761 atcatcaata acgcgactaa cgtcaattgc cagctgattg aaagcatcat cacgatgacc
    14821 ccaatcaatg tgactgcact agttacaaat tgagtcaaat tttgctgcag cgtgcttgcg
    14881 atgttatcca tatcattgat tgctcgagac atgatatccc catttgtatg tgtatcataa
    14941 taggaaatcg gtacacgatt cattttggct tccaaatcat gacgtaattc ataaactgtt
    15001 cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt tgaatacagc agaaatcaaa
    15061 tacatcacga ttactgtcgc aatgatctgc gcgattttat caaagtctat cggaaatttt
    15121 gttacttgtt ggccttgttg catctgttga gcgccagcca ttacaccttt gaatatttct
    15181 gtggttgctt tccccaagac ctttggtgtt tggatttgga agatcacggc tgctattgct
    15241 agtacaaata cagcgatgat cgcaaccata cgtttagaca tatagccaaa caatcggcgt
    15301 accgttcccc agaaattttt aggcttttca ccttttacac cgagatttct gccaggtcca
    15361 ccatgtcctg gaccgcctgc tggtccatga gagggacgtg tgtttgtttt actcatgcta
    15421 agtcctcctc tcttagttgt gaatgaacga tttcttgata agtttcgttt gttgctaata
    15481 gttcttcatg tgttcctttg ccaaccagtt ttccttcatc tagcacaagg atcatatctg
    15541 catccattac tgtacttact cgttgagcaa ctaaaacggt gatactttct ttcatattcg
    15601 ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc caacgctgag aaggaatcat
    15661 caaacacata gatatctgct gttttgacta atgcccgtgc aattgccaga cgctgtcttt
    15721 gtccaccaga gaagtttcct cctccttgtt ccacatggct atctaagcca tcttccatct
    15781 ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt ccaaatcatt tcatctgttg
    15841 catccggctt accatactgc atattgtctc ggatcgttcc agagaacaga actgcttttt
    15901 gaggaacaaa gcccattaat tctcgcaaat tatattgagt catgtcacta atgtccgttc
    15961 catttatttg gatcgaacca gattcgatgt catataatcg agggatcaaa ttgactaagg
    16021 tcgttttacc tgaaccagtc ccccctatga ttgcgacgat ctcgcccgac ttggcttgga
    16081 aatcaatatc ttccaatgca agtttttctg ctccatgata tcgataattg acatgattga
    16141 aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt aggattttca gggtcatgga
    16201 taccgatttt ttcatccaac acttcattaa tacggtcagc tgaagcttgt gcacgaggca
    16261 ccatgataaa aatagcggaa agcatcataa agctgatcaa gatctgcatc gcataagtca
    16321 taaatgcaat cagattaccg acttccaaag tcatatctgc tatgtagtgt ccaccaaacc
    16381 aagtgattgc tacgtttgta ccgctcatga tcaatgtcat cattggcatc atcaatgcga
    16441 cgatcgtatt aactttgatt gccgtatttg tataatcttt attcgctaaa tcaaaacgat
    16501 tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct gacccctgtc aatccttctc
    16561 gaaataccag gtttaaacgg tcagttttct tttgcatact tttgaataat ggaacagcaa
    16621 agtacatgat tccgccgatc aaaataatca ttactgggat gacaactaag aagatttttg
    16681 tcaactgatg atctttttga taagcaagta tgctcgcgcc aatcaacgtg attggtgcat
    16741 tgatcatcat acgtaaaaac atttgtgtaa ccatttggat ttgagtcaca tcattggtcg
    16801 ttcttgtgat caaagaggcc gttccatatt tatcaaacgc atcttttgtc agactctctg
    16861 attttttata gacatccgta cgcaattgct tacccagttt ctgtgattca cgagtagcaa
    16921 aaaatgtatt tgcgatagca gctaagatac tgatcaaaga aaagccgatc atcacaaaac
    16981 ctgtatgcca aatataatcg acatctcctt gcgctacccc tttatcaata atattggagg
    17041 taagtgtcgg caaatataaa tctgccagta cttggatcac catgaaaact gcagctgcaa
    17101 ttgctgaagt caaggagatc cgttttacga tttttagcat ctagttccct tctttctatt
    17161 tatatactcc gtttttcagc caagaaagtt tcaaattgaa ttcactgatt gcttgctcta
    17221 ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg cgcaattgtt gtaaatacag
    17281 tattcatcac taattggatc agtaattcca attcatgatc attttttact ttcagcttcg
    17341 tccgatcgat caattcaagc aactcttttg catcttgttt gtgttcttta tgtggatcgt
    17401 gaggcgtttt ttggcgactc gtatgaggag cgacttttct tgaggcacgg taatccatgt
    17461 tcataaacaa gttacgataa aaagaagcat tctcgccttc caaaatctca taaatcattt
    17521 tagaaaaata ggattcgaat cctttgaata gatcaccatc ggattctttc attagttgta
    17581 acatatcttt agagctgtca cgtctcattg tctcaaagta ataaaaataa agatcctctt
    17641 tatcttcaaa atactgatag aaactgcctc tagggatatc cgctaatttg acgatattag
    17701 caatcgaagc ttcttttaag ggtactctag aaaactcgat tctcgcagcc tcgattagtc
    17761 ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt cggcatatgg atcctccttt
    17821 ctttcaagat gacacattgt catatgacgc tgtgtcatta taacacctcc tacctgtttt
    17881 gcaagacatt tgcatagttt ttttcattat catccgatac attaaaaaag tgataagagg
    17941 aatccttcca tttgaattaa tgagagttgc ccattaaaat agccacatac taaacaaagg
    18001 atgagataaa tggcccaatc aattagtgtc caagacttca ataaattgcc tctcgatgct
    18061 gacagcatcg ttttagatac aagaaatacc ttagattaca caaaaaacca cttaagccac
    18121 tcgctttcta tccccgctgc tatcttgcca agacaattaa aaaatctaga tccaaagaaa
    18181 ctttaatata ttctaagcta tacaggcagg cgatcagaag cattagccga tcaacttact
    18241 gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg aagctcttca gtcgagtgtt
    18301 gcttgaatat tgcttgttag attcaaaaga ggctgtgaca aaaaatcgtg tcacagcctc
    18361 ttttataccc tgtctttttt tagttattta ttcttcaacc ttatcaatat gacttgatag
    18421 ccttttcttt ttctcatagt tataacaatc atagcaataa aaggagaagg taagcatatg
    18481 gcatctgttt tagtagtcaa aggtcatccc ctaactgcag aagaatcgcg cacagttaag
    18541 gcattgacgt cttttttaac aagttataaa gaaaatcatc cagatgatga agtgactgtt
    18601 ttggaacttt atcgcgatga tattccggaa atcgatgaag aattattatc aggttgggaa
    18661 gcattacgtg ctggtgcaga attcacttcc ttatcagaaa gtcaacaaca aaaaattgct
    18721 cgtttcaatg aattgactga tcagttcctt gctgctgata aaatcgttat cgcaaacgcc
    18781 ttatggaatc taaatatccc aacaaaatta aaagcttggt tcgataccgt caatgtagct
    18841 ggaaaaacgt tccgctatac agaaaacggg ccagaaggat tagtgacagg taaaaaagca
    18901 ctgcacatcc aatctaatgg cggtgtatac aacggacaag actttgcctc tcaatatgta
    18961 aaaggtattt taaactttgt aggcatcgat caagtagatc agctattcat cgaaggaatc
    19021 gattatgacc ctgaccgtgc agatgagttg atgcaaaatg cattagataa agctgctgct
    19081 ttaggtaaat cattttaaaa attgagataa atgcatacaa aagagacaga ggaaaaaatc
    19141 ttcaaagcag catttgtacc ttggatattt tcccatctgt ctctttttgt tattctttct
    19201 gctttttcaa cggattttct tcataaagag aagagattgt tgtgtaccat tcaaaaacat
    19261 gagtaatcac tactttagct gctgcataaa caggaatccc caagatcaca ccgatgattc
    19321 cgaacaattt cccagaagtc agcagaacta ataaaatcgt tactggatgg attgctaact
    19381 gattccctag tactaatgga gagatcaagc gtccttctat tgtctgctct actgcaaaaa
    19441 cgatcagtac tttgatgatc atgaatggac ctgctacaat ggctaaaaag atagccggaa
    19501 tcatcgctaa aaatgatcct aagtaaggga tcaggttcaa aaaacccgca ataatcccca
    19561 acgtgacagc atactctaaa ccaatgaccg caaaaccaat cataaacatg attgccacag
    19621 caaaagccac agttagttgt cctcttatat aagaagaaac ttgactattc atttcactca
    19681 gcacattcaa cgtcggtttt tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta
    19741 attgtttacc gtctttcaat agataaaata agataaacgg catcgtcagc agagccacta
    19801 aaatagtcgc taccgcgccg acaaaacttc caagactctg aactgtactc ttagatatat
    19861 cttggatcat atcgcctgcc gaattcatga acttatcacc tgctgtttcc aactgttcgc
    19921 gaaattggtt gaacagcggg tcccgcaaaa tctcggttaa tttattatca atcgtatcta
    19981 catattgagg gaagttactg atgaagctca ctgtttgttc ctgaatctta gggatgatca
    20041 ctacgctccc ccatgcgatt agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa
    20101 gccttggtac ttttcgcttt tcaaagtaat caactaccgg attcatcaag taatacaaga
    20161 ttcctgctaa aatgataggc agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca
    20221 aatatgaaac ttttgtaaat aaaaagacga tcagcaatat taacaataca acaagtaagg
    20281 ctgtgactac ttgattattt aaaaaccagc gccaaaacca cgataccggt ctgttcttct
    20341 tattttttcc ttccataatt cctcctgtta gttataaaaa atttggcttc ccaaaccaat
    20401 agcaggcagt ctatgcggat gaagatagat tccatttgca atttgtcctt cttctggaca
    20461 agcatcaaaa atcaatgata cgtgagctac tgtctctaaa ttcccattgg ccatcggacc
    20521 gacataatcg atcgtatata cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga
    20581 aaaaggttcg tcttttgata cttcttgaat caccgaatat tttcgtaagg cagctgttgc
    20641 tgttttatcg aacaaaatca gcatgggttc ttccttgttt attgcttctg tcccaatttc
    20701 tgtaatcttt gcctgaatca ttgcactctc tcctttaatt gctttcaaaa acagtataac
    20761 acattctcat tctttagccg tgtggaataa ttatgtgata gaataaaaac gaaaactatt
    20821 tcattacaat taggaggaat cagctcatat gatcgaaaag ataattttag gaacctatac
    20881 gcgtcgcgaa agtaaaggaa tctatacgat cgaccttgat acagaaaaag aagaattagc
    20941 caacctttca ttagctgttg aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg
    21001 caatctttac acagttacta gtgtggatgg attaggtggt gccggtgcgt acgataagga
    21061 ttttcatttt ttgaatgcgg taacagagtc tggcgctcct ttgtgttatg tagcggttga
    21121 tgaagcacgt caattactat acggggcgaa ctatcataaa ggtgaagtaa atgtctacca
    21181 tatattagga gatggcgaac tgaaagcagc agattcaata ttccatcaag aagcgacagg
    21241 tcctcataaa aaccaagatc atgcgcatgt ccactatacg gatctgactc ctgaccaacg
    21301 attagtcgta tgtgatcttg gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg
    21361 caaattgaat cttgtaacta ccttcactgc agagccagga acaggccctc gccatcttgt
    21421 ctttcatcca aatggacaat tcgcttattt atttggtgaa ttagacagca cagtccgcgt
    21481 gctttcttat aataaagaaa caggtgcttt tgaagaatta cagaaaatat cgaccttgcc
    21541 agaagaattc gacggtgaaa atggaggggc agctatccgc atttcaaatg atggcaagtt
    21601 cctttatgct tctaaccgag gacataactc gattgctgta ttcgcagttt ttgaagatgg
    21661 ttcccacatt caaaacaagc aaatcatctc aacagaaggg gattttcccc gcgatttcgc
    21721 actagatcca agcaataatt ttgtcgtgtg tgccaatcag aacaccgata atctgacatt
    21781 atatcgccgt catcctgaaa caggattact ggaattactg aaaaaagatg tctatgtacc
    21841 agaatgtgtc tgtgtttatt ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca
    21901 agacgtatta gacagactac gggaagaact aaatattcct ttctttgatg ggaaaatcga
    21961 agataaagag tatagcgaag aggaatatca gaaactcaaa gccgatttac agaactactt
    22021 tgaacaatat gtacagaatg tagaaaacta atcagctttc catactatct gaaaagaaca
    22081 tcaggacaag acttttccgt ctatcctgat gttcttttct acaaataaaa aaccgccgag
    22141 atggttagac tcgacggaaa aggagtattt actcgatttt gagtaagtac aaggttgttg
    22201 ttggttatag gtatgtcatt atcataaagg attttgatca ctctatcata aaggtaatat
    22261 tacatttata ttagataact ctagttttta ttgttatcaa agatttcttt tattttttca
    22321 acaacaaatt agtcattttt tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt
    22381 ttgcttcaga tgtattgaat agatcttcat ctacccctga ttcaagatcg atactttgga
    22441 tcatttcttc acagcctgct acatatgaaa gataagcttt ttcaaaattt ttatgaatgc
    22501 ccattgcttt tgctggtgga cgcaaagtac tgactttctt aagcattgcc cgatattttt
    22561 ttgttccttc ttcaaataac aggatgatct cttcaagagt cgatttgtcc agttcacttg
    22621 tttttccttc atcaattgcg tttctgatca cttcatattt cggatgcatt ccttctccga
    22681 ttgcttctgt ttccttgacg atttgatcga tggactgcac ataatagcca atattcggta
    22741 acatgttcgt tttcctactt tctccattgt tgttattatt tattctactt gtttttatcc
    22801 tcaaatgaca agtacttgaa aaaatttaat ggtttctata cagctcgcca ttcttattcg
    22861 tgctcaaaga aaccatctgc taaactatat tgtttccctg cgacaatgtc atattggatc
    22921 aattgatatt cgtcgtaaat ctcttgttgc gagcgattga attgcgcttc tttataccac
    22981 tgtccgtttt gatagtacag ttctcgttca aacgccggaa gttcctgttc taatgataaa
    23041 agcagctgat aaaagccatt tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa
    23101 taaaacggac tggtgattgc atcgtgagtc gtttgctttt ctaattctcc tttactgtcg
    23161 aacataagaa attgtgtttc atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct
    23221 gaatagatcc ctggaagatg atccccccaa aaaacaacta aagtccttct agaaagattc
    23281 ttcaattctt ccgtgaactg cttcagtgcc gtgctgctat aggaaatatc ttgcagataa
    23341 ttctctaagt caagtgtccc acttccatct gaagtttttg caggatagtc tagattatca
    23401 tactttccat cgtagggcat atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt
    23461 ttttcttcct tgagaagtgt cattacttct ttgtaagctg actcatcaga aatatatgga
    23521 ttattctcta tcgtatccgt ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct
    23581 aatgtctgat aaacatcctc tcttttatac atcgaagtat tgtatggatg gatcgctgtc
    23641 gtctggtatg attgtgcgtt taacgctgaa acgatagaag gaagctgatc catttttggt
    23701 acaagcatcg tgtaaggggt ggtgagttgc gcattaaata acgccataga aaaaccagtc
    23761 aatgcttcaa attcgatatt tgctgttcca ccgccatagt tttgagaaag catattgcca
    23821 ctatatgttt gatcagctac ttcataataa tctgccaatg gctcaccaga tacttctacg
    23881 ccgtttaatc gactaggatc tgagaaactt tcactcatca cgaacacgat gttcggcgac
    23941 tcttcctcta cagttttatt tttttcatcg gctagcttct ggtatttttc agtgatttct
    24001 ttgatctttt ctttcgaata tccttcaggt tcatccattg gttctacctt cagattgtac
    24061 aaaaacccac cgataaaacc agtgttatag tagttcattt tctggctgta aggtatccat
    24121 agtgccgttt tattatatgc ttttcgcaac aaattatcgg gattgttgaa attactgata
    24181 taccctagca gtccaattgc cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt
    24241 ccttttttca ggcttcgaaa catgtaccag caaaacaatc ctatcacggt tcctgcggca
    24301 gctaagatca ctgtaaacag catagtgccg gtcatatctt tcagcaatcc gatttcagtg
    24361 atcatcttca aatcgtctgg ataaatcggt tcttgccgat aactcatttt catataattt
    24421 gcaaaaccta atacaccgat ggctacgaca tacgtaaggg aacctaaagg gacagatcct
    24481 gctagtgcaa tcagaaaaat aagaataatc agcaagacga gacaggctaa gaagaatttt
    24541 tccgtatgcc aagaaaaagc aaatttcaac gcaaggtcta cggatagatc attttgacac
    24601 cactgcaaaa ataaattact aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt
    24661 ccttttaaaa tctttttctg tttggtcgaa agtctgctta tatatctttt caaatgcttg
    24721 ctccttcatt tctatcaata aaagaatgtt gcttctctta ttttagttgt acgaaattcg
    24781 aaagtaaaga acggaaagaa gaattaatga aatccaaata ttctctttct ttatggcctt
    24841 tctggtttct ttaataagcg gacctttcgt actccagaaa ttttttctag ctgttgtttc
    24901 acttgattga gctgttcaaa atcagtactg tctatgtcaa tcaatatgta gacatgctca
    24961 ttcttatggt tacgcgatat atctgcgatc gctagctcgt tctcattcag cagagaaaat
    25021 atctctgcta atatattggt tttcttttga taaaatatag tgaatcgaaa cggcgcttgg
    25081 aaaaacattc gtgcggatgg ataattgacc gcttctctaa ccgtaccgaa caaaagataa
    25141 tcttttaagg cttcaactgc caaacgtccg ctgtctgcca aagcttcctg agtcgatccc
    25201 cctagatgag gcaacataag tatacggtca ttttctaaga actcttcttc tggaaaatca
    25261 gtcaagtatt tagccagatg ttgtttttcc aatgcttgca taacagcata tttatcaaca
    25321 atttcacttc ttcctacatt gataagtaca gcatcttttt tcattttttc tatattcgcc
    25381 tgatcgatca atccttttgt atcttctgtc aacggcagca tgactacgat gtaatccgag
    25441 gtagaaagaa ctgtttctaa atctgcttgc cgaacataat ccaactgggc atcgcggata
    25501 gaataaccca agacatccat ccctaagcta tagcaactta gcgctacttt tgtgcctatc
    25561 gctccaagac caagaagacc gatggttttg ccttgcaatt ctcgtcccac ataagcactt
    25621 cttttgtttt ctgcctgttc taaaatatta ggacctgtta gtgtttgtac catacgactt
    25681 gcttcgatta tcggacggga agataagagc aaacagcaca aaacaagctc ttttactgca
    25741 ttagcattta cacctggtgt attcatgacg atcgtcccgt tttcagaagc tttttcaacg
    25801 ttgatcgtgt tcaccccaac accagctctt gaaattgcta gcaaatctgg tgtaagccat
    25861 tcatctttca ctttcgtact acgaataact attccttcgg ctggcaaatc atctattatt
    25921 ttgaaatatt ccccttgtaa cttttctatc tcttgttctg gaatagcatc aatcaaatga
    25981 acgtgataca taaaaccccc tccttactaa caagtatctc gaaatttctg caattagaca
    26041 actgtttacc atttgacttc ctagaagcaa aaagagcagt tagagcagag taggctgagt
    26101 gataaaaata aggctatgac aagttactgc cataagcctt attttcgaga gcaaaatatt
    26161 gagggtgaga tatcttgata aatggtgttc cgttagctga cattgataat aagactaaat
    26221 ttcaaaaaag ttgaaaagca aatttcgtca atctcttctt atttcttaaa actaagtggc
    26281 tttttcacaa cctcttttta tctaataacc actgtttcca actcaactaa ttttgcccac
    26341 gtttcaattt gatctgttgt tagatttagc gagacaacgg tatgatgacc gccacctgcc
    26401 tgaatccatg aatggacacc ttcatggaaa ttcggtttag ttttccataa cacacgtgca
    26461 acaggtaaat taggtgcttc agtcgttggt tcaaaggctt ctacttcatt gatcagcagt
    26521 ttataatgcg taccgaaatc tgccatcgat acgactaccc cttctcctgc tttcccatca
    26581 aatacaagac gcgccggatc ttctctatct cccattgata aaggagaaac aacgattctt
    26641 ggtttcgtcg cagcaagcgt cggatctact tccatcatat gggattccaa gattgcttct
    26701 tggccactag ctagctcgta agtataatct tccatgaatc ctgtatcttt ccctcgagcc
    26761 atgattttca gcaaccgatc aatcgctgca gttttccagt cgccttcccc agcaaaaccg
    26821 tatccttctg ccatcaaacg ttgtacagct agtccaggta gttgtttcat tccatacagg
    26881 tcttcaaaat tagtcgtaaa agcgttatag cctccttctt ctaagaaacg gcgaataccg
    26941 atttcctgct gagcttgtac ttttacatgt tcctcccatg tcttttcttc ataatcacca
    27001 tagtcgaagg tatacaattc cttgtactct tcaaataatt gatcgacttc ttcgtctgat
    27061 acgcgatcga ttacttgtac aagatctccg ataccaaaat aatcaacagt ccaaccaaat
    27121 tgaatctgtg cttccacttt gtctccttct gtgacaccca cgtttctcat gttgtcacca
    27181 aaacgagcca cttttattcc aaaactttca ttataggcta ctgcaacatc catccaatca
    27241 gcaatctctt tttggatctc aggtcgtttc cagtagccaa cgacgatttt attttgttta
    27301 tttaatcgag cattgatgaa cccatattcg cggtcaccat gagcggattg atttaaattc
    27361 atgaaatcca tatcgatcgt tttccaagga atgctttcgt tatattgcgt agctaaatga
    27421 agcaatggtt tttgtagaag ctttgtcccg cggatccaca tttttgctgg tgagaaagta
    27481 tgcatccaag tgatcacacc tgctacttct tctcgataat tgacctcttt catgattttt
    27541 gtgatcgtat cagcagtaac tgctaattct tgaaggcgga ttggataagg caattggcca
    27601 ttttcattca gttcatcgac catttcttgt gcatgtttct ttacttctcg cagtgcttct
    27661 tccccatata aatgctgtga tcctacaaca aaccaaaatt ctttttctcc aatattcaac
    27721 ataattagcc actcctttta tttgttttgt ccgtaatacg cattttttcc gtgttttcgt
    27781 aaataatgtt tatctaaaat ccgttgaggc agctcttctg aaaatgaatt gagctgtctg
    27841 gtaaataaat tcatcttgca tacttcatct agtacgactg cgttcattac cgcactttgc
    27901 gcatctttcc cccaagtaaa tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag
    27961 ggatcaattt tgcgttcttt gaatgtttct atgataactt ttcctgtttc atattcgtac
    28021 ccgctgtcga tttcctgttg tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta
    28081 tctgcatgcg tcgtccccat cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc
    28141 catgtcgagt gagtatggac gattcctccg atttgaggaa atgaacgata taaaacagca
    28201 tgagtaggcg tatccgaaga gggattgagt ttaccttcaa tgatatgatt gtccaagtcc
    28261 acaacgacca tgtcgtctgc tgtcatctct tcataactga ctccgctagg tttgataacg
    28321 aaatatcgtg tttcaggatc aaaagcgctc acatttcccc atgtatattt gacgagtccg
    28381 tgtttaggca agtcaagatt tgcttggtat acttcttctt tcagttgttc cagcattttc
    28441 tatcactcct ttttcttgca attgatgaat cgctgttttt tcaatgtcga tcccctcacg
    28501 gtaacgtctc ataaatatct gatagcctgc tatttcttct gctgaaggtg tgatggtgat
    28561 tccttggtct tgtccaaaca cgtcttctgc caagaagttt tctaaagact tcccatgtcg
    28621 atccatcata taggctgcta ataacgcgat tccccacgct ccaccttctc cggctgtgtc
    28681 catcactgta actggtgcct ccatagcaga tgctagaatc cgctgtccaa cttctggtgt
    28741 tttaaaaatc cccccatgcc ccactaaacg atcaatttgg acattctcag atttcaatat
    28801 atccattccg attctcattg caccaaaagc actggctaaa tggatccgca tgaaattcgc
    28861 taaatcaaaa cggctacctg gtgtacgtac aaataatggc cttccttcat ttactcctgt
    28921 aatgttttca ccggaaaaat aaccataaga aagtaatccc ccgcaatccg tatcgccttc
    28981 tagcgctttg ttgaataata ccgtgaataa tttatctctg cttatctcca tacccaaaga
    29041 attggtaaat tcttcaaaaa tactgaccca agcattgata tccgaagagc agttattcgt
    29101 atgaaccatg cctactagtt ctcctgaagg tgtagtgacc atgtcgattt ccggatggac
    29161 attttctagt tctttttcta atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt
    29221 ccctgtccgt ttggctacac tgttcgtagc gaccataccg gttcctgcat ccccttctgg
    29281 cggacaaacc gggattcctg gtctcaaatt tcccgtaggg tcgatcagat aagcccctga
    29341 ttcgcttaac ttgccagctt cttctcctgc tactagaact ttaggcaaaa tgtgatccaa
    29401 gtcaatcgct acgtgcgctt tttcgattaa ctgaccaaaa atatttttca tttcttgatg
    29461 gtaggttttc gttgaaggat cgataggaaa catccctgat gcatctccta cgcctagtac
    29521 tttctcacca gtaagctgcc aatggacata acctgcaagc gttgtcaaaa aattaatttc
    29581 tttcacatgt tcttctttat tcaaaataga ttgatacaaa tgagcgatac tccatctctg
    29641 cggaatattg tattgaaatt tttccgttaa tattcgttct gcttcttcag tgattgtatt
    29701 tcgccaagta cgaaatggta ctagtaactg ttccttgcta tcaaatgcta gataaccgtg
    29761 catcattgca ctgaacccaa tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa
    29821 tacttctgct gccatttttc ggtaactgac gcgcagtcct ttccaaatat cttctaatga
    29881 ataggtccag ataccatttt ctagtttgtt ttcccactca taactaccag cggcaatagg
    29941 agtaaaagat gaatcaatca atacagcttt gatcctagtt gatccaaact caatccctaa
    30001 agatgtacga ccatctatga catctaacat actttgcgca tttacttcct tcataatgat
    30061 ttaactccct ccttctcgtc aaacctgatc aaaccgtctt tttagtagcg cttacaatga
    30121 ttattgtata cttttgtacg tacatgtcaa gcggttattt acaagaatac aataaaaaac
    30181 acttgttttg tacgtacata agttataaaa tgaaattgct tacaaaatct gatcaaccgc
    30241 cttctcgaaa ttctagaagg agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc
    30301 gtttatttat ttttttggcg catttggcgg cattctattt ggatatgata ttggtgttat
    30361 gaccggcgcc ttaccttttt tacagcatga ttggggtttg gcaggtaaag cttctttgat
    30421 aggatggatc acgtcttcgg tcatgttagg tgcgatcctt ggcggctctt tatctggtct
    30481 tctttctgat aagcttggac gacgaaaaat gattcttctt tctgcactta tttttatggc
    30541 tggttctgtc ctttctgcat ctgcccctca caatggttct tattttctga ttgcagcaag
    30601 gattcttcta ggtttagctg ttggcgctgc atctgcccta gttcctgctt atatgtctga
    30661 aatggctcct gctagactaa ggggaagatt gtcagggatc aatcaagtga tgattgcttc
    30721 tggcatgctc ctttcctatg ttgcagatta tttgctaaaa gtgttgcctg aaacgatggc
    30781 ttggcgcgtg atgttagggc tcgctgctgt ccccgcgctg attttatttt tcggtgtttt
    30841 agctctacca gagtcaccgc gttttcttat gcaatctggc cgcttggaag aggcaaaaag
    30901 agtattgaac tatattcgga caccaaaaga agctgaacag gaattcgaac aaatccagct
    30961 gaatgtcaaa caagaaaaga caaccggaac ttcttggcat actttgtttt tagaaaaata
    31021 tcgttcctta gtcattgccg gtattggtgt agcagtattc cagcaatttc aaggagctaa
    31081 tgctattttt tactatattc cgctgatcgt agaaaaagcg actgggcatg cagcgagcga
    31141 tgcattgatg tggccgatca tccaaggaat cattttagtt gctggttctt tgctcttttt
    31201 ggttattgca gacaagttca atcggcgaac tttattgaaa ataggcggta gcgtgatggg
    31261 actttcattc atcctaccag ctgttttagg aactgtactg gatgctcata caaattcgtt
    31321 gttgattctc ttgtttttat gtatttatgt cgcattttat tcttgcacat gggctccact
    31381 gacatgggta atcataggcg aaatattccc actagctgtc cgcgggcgag catctggcct
    31441 agcctcttct tttaattgga ttggttcttt cctagttggc ttgctttttc ctgtgatgac
    31501 tgcttctatg tctcaagaaa tcgttttcgg catttttggg atcatctgct ttttaggggt
    31561 tttatttatt caagagatcg taccagaaac tcgtgggaaa tctttagagg aaatcgaaca
    31621 aagtgcatct aaaaaaactt atcctaaacg tatttctatc catcattctt aaagaggttg
    31681 tgacagaagt ggtcagcttc aagaaataag aaggaaccgc ttagtttcca gtcacaagga
    31741 acaatttcaa aaaacagctt ctcatgtttg atgaatattg tgacttgtga ccagttggta
    31801 ggagaacacc gtttagtgga gaggtggtga aagaaacgtt cagccttgag cgatgacaaa
    31861 agctctcaca aacattgcga cgagtaaccg taggagcaat aagaggcggt aaaaacccgt
    31921 ctcttatttt tcgttttctt tcactgtttc ccgttcaact agtacaggtt catagagaat
    31981 atttggctgt gcctgtcctt tttcaatcgt tgtgacgatc caccgtgcgg catcttgtcc
    32041 caacttttct tttggatgcg tcaatgttgt caatttttta ttccctaaat ggcttaaaga
    32101 agaatcgtct gttcctacga tcgataggtc atctggaata tgatagccat tttctgtcaa
    32161 cttatctagc attaaactag ctatttgatc gttatagcaa atgatgccat ctaaagcttg
    32221 ttcttctttc aaccgttgta ctgttttttc agcgattgtt tctcgtgttt ccgtggtgaa
    32281 agtaatgata tcttccgtca aaaaacgcac attatactgt tcacacgcgc tgataaaacc
    32341 tttcatcctg tattttcctt gaagatcgtc gatttttgtg atgaacaaca gacgctgatg
    32401 tccttttttt atcaaatatt ctgctgccat aaaaccgact ttcacatcat ctaaacaaat
    32461 ataagaggca tttaactctt cataagcagc attgatcatc accatcggaa tgccttgttc
    32521 tctcaaagca acataaagtg ctaagtttgg gttatactga ttacttttcg taggctcaac
    32581 gatcaaacca tctaccccat aagcaatcat tttcttcaaa cactctttct ccaattcgtg
    32641 atcgttattc gtactggcta aaagcaatga atacccagat tctcgtaatt cattttcgat
    32701 tcctcggata atcgacggaa aaatataatc agagagatat gtggtgatga caccgatcgt
    32761 ttttgttctg ttcaatggtg ttttttcttt cttatagctg tcatctacat atgtgcccga
    32821 ccctttttct ttacgcagat agccctcatt taccaacaca gcgattgctt gccgaatcgt
    32881 atgtctgctg acgtcatata tttcttgcag cttgaattct gagggaatgg ttgtatttac
    32941 ttcgtattcc tcacgaagga tcttttgttt tatctcttct gcaatatctt gatattttgc
    33001 catttgcctt gccccttttt atttgtacgt acatatatca taacgagtct gcaaggttta
    33061 ttcaagcaaa aatcaaggtt tcttgtgctc ataaggcata cggaacaggt tcaccaaatt
    33121 ctggaaatcc ttgctcattc cacgtaaagc attgcgcgtt cgcgtgccga ttaggattgt
    33181 ctaacggatc ccctttttgg ttcttctctg gtcgagcatg atagatcaaa atatcaaagc
    33241 acccgtcttc tgaaaccgta aacgagttgt gaccaggacc aaattgctta tttttttcag
    33301 aagaaacgaa tactggctcc gataatttat gccatgattc ttttaataaa agattcttgt
    33361 gctcatcagc ccacagcagg cccatggcat agttttcatc tgttgcacta ccagaatacg
    33421 taatgaatat tttcccattg cgagtaagga ctgctggacc ttcatttact gaaaaacccc
    33481 gtttttccca ttcatactca ggtatagaaa ggaggatctg cttccccttt aacgtccaag
    33541 gattatccat ttcggataga taaagattag aattcccagt aatttcagga tcttgttggg
    33601 cccacacata atatagcttt ccttgagaag agaaagttgt tccgtctaaa ctgaaagttt
    33661 cccttttcgt ttttatttgt cccttttcta tccaatctgt attcattgga tctatatgct
    33721 gattttcaag tacgaacatg cggtgatgat gagattcatc acgtatagtt ttatgattgc
    33781 ttgcagcaaa gtaaatatac cattttccat tgatataatg gacttcaggt gcccagatca
    33841 attcactcat cggtccttct tcatgagcct gccatacaat caggcttgca gcatgttcta
    33901 attcattcag tgactttgcc cttcttaatt caatcacttg gtagcctgga accgatcctg
    33961 taaaataata ataaccatct gtatgtttat atacccaagg atctgcccgt tgccgaacga
    34021 taggattatt ataaagtgtc ttcatcataa caaatttcct cacattttct ttattctaat
    34081 tacattccaa gagtattttt ctatccctac atttaattcg tcatgttttt ctattgaaaa
    34141 atagtttatt tcaggtttta ttttttctgg ttcttcacgt gtatttttgt ccaacagatc
    34201 gctactggtt aatgtgtagt gctctccttg atctagcaaa ttgaaatcat gttcaatcgt
    34261 tatcttttgt tgttcatttg tgcgattgat caagaaaata gtcagctgat tgccgttatc
    34321 tacgatcaca ctgtccacat aaggaacatt ttttatcttt tcacaatcat aaacagggca
    34381 aatgatttgt tgtttcaata cttgaccttt agcatatttc gataagtgtt gcatcacata
    34441 ataagttgga ttcctccatg cttgtccttt agcatctgtc agtattaaag gtattgtgtt
    34501 tactaacaat gattgacaac taatctttac gcgatctgcg tgtcgtaaaa tagccagccc
    34561 taatgaacca aataataatg tatcttccat agtaaagtaa caccaatcga tgggactacc
    34621 gacttcaaaa tccctatgtc ttttttccgg ttgggcatgt acattccatt catcaaacgc
    34681 tagatacatt gttttctcac ttctcaaata tccttttaca taatcacaag tagcaacgat
    34741 ttcttcaatt tgacgatcga atcgataagc ttttgcaagg tatgtcgctg tatcatcacg
    34801 ctcattttta ggaggtttag tcaagtcttc ttcatcatac ttatcaatat agttatgcaa
    34861 agctaaataa tctacattct cataagcttc ttctaatgcg atacgatccc attcagggta
    34921 cgatggtaaa cgtggcgtag agcttccaac taagattgtt tcaattgaat catccactaa
    34981 cttcatgact tttgaggctt catttgctaa cctaccatat tcataagcac tcttagcccc
    35041 cacttgccat ggaccgtcta gctcattccc taaacaccat aatttaacgc caaaaggctt
    35101 tttttgccca ttttttattc ttaaatcact ccagaaggtt ccttttggaa aattacaata
    35161 ttctactaaa ttttttgctt catcaattcc ttttgttcct aaattcaccg tgaatatcgg
    35221 tttcgcttgt acatgactga tccatttaaa aaattcgtgg agtccaaaag aatttggttc
    35281 tatacctcgc catgctaaat caattttttt tggtcgttgt tctttcctac ctacagtatc
    35341 cacccaatca taaccagatg taaagtttcc tccaggatag cgaatgacac ctaattctaa
    35401 ttcctttaca taagagagga catcttgtct aaacccgtca ccatcagctg tttcatggcc
    35461 cggttcataa atccctgtat agacgacact tcccatatgc tcaacaaatg agccaaacag
    35521 ccgagaactg atctctccta ccgtaaaatc ctctctgatt tttactttca cttcttcgtc
    35581 caacagcttt ccctaccttt catttcctat agtcaagata atagatacaa gtttccatac
    35641 gccaccagca aagataaaaa taaaaggagg gaaaagaaat atgagcagta caaatcctaa
    35701 taacaatacg caacaaagaa tatttctcaa aaaatcaaaa aatactttcc atgcactttg
    35761 ttcaaaaatt tgtaaaaatg tgatatcttt gttttcagaa atcacatata tccaggataa
    35821 aagaaacatg gaataaataa gtaaaactac atatccacta attgaaagta cgattgtctg
    35881 ttcttctaat aattgattaa caagaaaata aataatgatc gttaacagac taaggaacaa
    35941 gttcttacca aaatgttgct ttattccttg aaagaaaaaa taaatgatat ttcctgatcc
    36001 ctcacctttc cattgcgcca ttacataaaa aacagcacat gtagcaggca cgatagtaat
    36061 aacaggcaaa cacacgatta cccagagaat acctaataag atgtaatcag aaaccacaat
    36121 caactgtcca taaaatttag tctcaactat tttcatcttc ctcactcttt cattccagac
    36181 atagctgttc cttgaacaaa atatttctgt gcaaaaatat aaaccactag taatggcagc
    36241 attgaaataa ccgttccagc cattacaggt ccataaaaag aaatgttctg ccctgtaaat
    36301 aaagctaacc ctgctggtaa agttctcatt tttgaactac ttgtcaatat caatggataa
    36361 agcaagtcat tccaactatt catcagcgtg aaaatcccta atgataaaag agtaggtttg
    36421 ctcaatggca acatgatttt caaaaaaata ttaaactcac ttaatccatc aatgcgtgct
    36481 gcctcttcca aagaatctgg taaagtaata aaaaaggaac gcatcatgaa gataccaaat
    36541 gcagttgtca tacgaggaat gatcaaacct gcataagtat caagcatgcc aagcaaattg
    36601 acttcaataa atagaggaat catgaagact tggaatggga tcatcattgt cagcaaaacg
    36661 aaatagaaca aaactgattt tcctttgaaa cgcatacgag caaatgcata ccccgcaaga
    36721 gaatcaaaga atacagaagt gatcactgtc cctccagcaa aaatcactgt atttttgatg
    36781 taatctaata aagggatcgt tgtccaaacc ttaatatagt tatctagggt atagctatct
    36841 gcaaatattc taggcggaaa tgagataatg tctttttcgt gtttaaaaga agaaataatc
    36901 agccacaaaa aaggaaacgc aataaccaac aatataacta gcatactaat cgttcgaatg
    36961 atttttttac cactaactgc ttttcgacta ttcacagtaa ctgtaatcga gctttctggt
    37021 gccatacagg tttccctcct atttcatttg tttttctttc cgtgacatgt acacattagt
    37081 ggagatagct atagttgcaa caatgataaa caaaaccgaa gacagtgctg atgcatagcc
    37141 caattcataa ggtgctgtaa atcctcgact gtaaatatat tgaacagctg tttctgtctt
    37201 aaattgtggg ccaccagcgg ttgcaacata tacttggtca aacacttgca tcgaaccaat
    37261 cagattcgtt aaaatacaaa aacctaagga gggaacgatc tgaggtagcg taatgttaaa
    37321 aaactgctgt cgatttgttg caccatccat ttgagcagat tcatacaaag agggagagat
    37381 tccctgaata gcagtcaaga gaatcgtcat agtaacacca aaattcttcc aaacagtcat
    37441 gatggctacc gtcggcatag caagggttgg atcgcgaaaa aattgaggga tcggcaagcc
    37501 gacttgtgaa aacaagtaag gaatcgcccc tacattcgga tcaagcaaca tagaaaaaat
    37561 aatcgcaatt gacgtcaatg aacaaataac tggaatgtaa aatgtcgaac gacaaaaacg
    37621 attgaatctg gtatttttag ataatgctac tgctgctaat aaaccaacga taatttgtgt
    37681 tggcgtttcg agtacggtga aatataaaga atgaatcaac gaattaactg ctcgttcatc
    37741 actaaatata cgtgtgaaat tttcaaaact tacaaaattt cttgatgtaa agaaaatatt
    37801 caaatctgta aaactaatag caaatgttcc taaaagcggt atgacagtaa ataataataa
    37861 aactaaaaaa gatggagtta aaaacaaata cgcagcacga tcaggacgat tccaatattt
    37921 ggtgaatccg ttcatatagt ccacctcatc tttcattttg ataaaatata caatcttggt
    37981 ttaaacgaat ctgaataaga tatccgaaca atcgctgtga tttttgcaat aacacctatc
    38041 tgcaaatact aggtcgatca ttcagatatc tgtatcttgc caacagtcta tcctgtataa
    38101 agttctgtac tatttttgca gtaattgatt gatggcatca tctgcatcgt tcatgagtgt
    38161 ctcaggatct tctcctgcta ataatttttc aatcagtgga ttgatgatat cgttattgat
    38221 cgcagagatt tgggtaaaac ctggtaaata tggctctgca tagtccattt gtttcgataa
    38281 ttcagataca ataggatcat tttttacttc ttcatctttt gctactgaat ccaagtaggc
    38341 aggaaaacca ttttcgatac tccatttttt accaatttca gttgtattcc aatatttcaa
    38401 aaactcatag acagcttctt ttttattatt atctgtactt gccggaatgg catatcctac
    38461 gccatctaaa atagctgttt ttttgtctga atcactccct tgtggtaagg tagtcacatt
    38521 ataatcaatc tcgtttgctt tcaatccgtt atttaaccat ggcccattga tttcaatggc
    38581 tagttgacca gcattcatta aattgtccat ttcggcgcca gaaatcgatt ctggaccagt
    38641 cttgtctaca tggatcattt tttggatcgc ttttaacgtt ttaaggttct caggagacgc
    38701 aaattcagac ttagtataat catcgttcac taatttaccg ccatttgcta acatccagtt
    38761 atataacggt gcagttccat ccttattgaa gacaaaacct gatacatttt tactactatc
    38821 agttaatttt ggtgccattt ctgtcaattc ttcccaagta gtaggtggtt gttcagtatc
    38881 taatccagct tgtttaaaca aatcattatt ccaatacata tacatgcctt gtacctgcat
    38941 ggggacaaaa tactgtttac cttctacttc acctaattca atagccgttt ctttaaaatc
    39001 agatttatct actccatcat attcccagaa atcgtctaat ggttgtaccg ctccgttttc
    39061 tacatactgt gcaaaatctg gataatttaa tgcaataaag tcaggtgctt ttttagaaga
    39121 aatcgctgta ggaagttttt cattcaaatt ggcccaagtc atgatatcca tttcaatttt
    39181 gatgttttta tcatttgttt tattaaattc attaacgatt tcttgcaata tttcaccatc
    39241 agatgcagta aacccattcc aaaaagtcaa agtcacttct ttatcagaat cagattctgc
    39301 tgaacttccg ccaccgcaag ctcctaaaat acctgttgca gctaccaaca aagagccaat
    39361 taaccatttt ttacctttca ttttttgttc ctccttcatc tgataaacga catctgacca
    39421 cattccaaga tagcggtttc aataaacaat tgattttccc atcttcagct tttacatttg
    39481 ttgaatgact tggttttact tgttcacttt taggaagatt agttgctttt gtatcaaatc
    39541 cactcatttc agaaaaatca atcactccct ctaatgataa ttcagacaag tgaacagtta
    39601 gattcatttc ttcttctcca cggttaactg caaatatgac aagttctttg tcatttatta
    39661 cggctatact ttctaaatac ggcacttctt caaaatcttc cgtcgcatag tttgctgaac
    39721 gaatttgtgg gcttaacacc tctccttgtc catacacagc agcttgcata aatggataat
    39781 agatcgtctg tctccaagct tctccgtttg tttctgtcct gattggtgca ataacgttta
    39841 ccaattgagc aagacaagca atctttactc tatctgcatg tttcaataaa gtgattaata
    39901 gacagcctaa tagtaatgca tcttcaaaat tatagttatc ttctagtaaa ggaggagcaa
    39961 tctgccaagg cgctgcttgc tcatcttgtt ccgctgaatg ataccaaata ttccattcat
    40021 caaaggacag gttgatctgc ttcttactat gtttcttcgc cttgactgta tcgcacatcg
    40081 ctgtcacttc agaaatgaac cgatccatgt ctaaggaacg tgcaagataa tgagaaagat
    40141 catttttttg gttgccataa tattgatgca gagacaaata atctatttga tcgtacgctt
    40201 gatcaagaac tgttaactcc caatcaccaa atgtaggcat ttgccgattc gaacttccgc
    40261 ataaaaccac ctcaattgaa tcatctacga gtttcatcgc cttagccgtt tcctctgcta
    40321 accgtccata ttcttccgct gttttatgtc caatctgcca aggaccgtcc atttcattcc
    40381 ctaaacacca tgtcttaatt ccaaaagggc tctcttgacc attcgaacgc cttaaatcac
    40441 tccaataagt tcctttagga aaattacaat attcgactaa attacgagct gcatctactc
    40501 cacgagtacc cagattgaca gccatattga tttcggcccc cacatgctga caccagttat
    40561 aaaattcatg aatcccaact tcattcgttt caattgtccg ccaagctaaa tccaaccttc
    40621 taggtctatt tgctatgggc ccaatactgt cttcccaacg aaatcctgac acaaaatttc
    40681 caccaggata tctaatcatg ggaatgttca attcttttac caagtgaatg acgtcttggc
    40741 gaaatccact tttattagct gatggatgat ctggctgata aatcccttca tatactgctc
    40801 gtcccatatg ttcaataaat gaactgtaca accgcggatc aactttgcct acacgcttga
    40861 cactactaat caacattgtg gcttccataa ctgacctcct ttaatttata catgaataat
    40921 acacctcgtt
//
LOCUS       DAFEPQ010000017        40085 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.017, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000017
VERSION     DAFEPQ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.017"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            366..1415
                     /locus_tag="LP543_001023"
     CDS             366..1415
                     /locus_tag="LP543_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL2321200.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            1412..2293
                     /locus_tag="LP543_001024"
     CDS             1412..2293
                     /locus_tag="LP543_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2321201.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            2296..3066
                     /locus_tag="LP543_001025"
     CDS             2296..3066
                     /locus_tag="LP543_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2321202.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            3345..4199
                     /gene="aroE"
                     /locus_tag="LP543_001026"
     CDS             3345..4199
                     /gene="aroE"
                     /locus_tag="LP543_001026"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBL2321203.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            4377..5540
                     /locus_tag="LP543_001027"
     CDS             4377..5540
                     /locus_tag="LP543_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL2321204.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            5657..6067
                     /locus_tag="LP543_001028"
     CDS             5657..6067
                     /locus_tag="LP543_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL2321205.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6241..8469
                     /gene="topB"
                     /locus_tag="LP543_001029"
     CDS             6241..8469
                     /gene="topB"
                     /locus_tag="LP543_001029"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL2321206.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            8640..8924
                     /locus_tag="LP543_001030"
     CDS             8640..8924
                     /locus_tag="LP543_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321207.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9084..9320
                     /locus_tag="LP543_001031"
     CDS             9084..9320
                     /locus_tag="LP543_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL2321208.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            9317..9715
                     /locus_tag="LP543_001032"
     CDS             9317..9715
                     /locus_tag="LP543_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBL2321209.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(10382..11326)
                     /locus_tag="LP543_001033"
     CDS             complement(10382..11326)
                     /locus_tag="LP543_001033"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBL2321210.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(11622..12566)
                     /locus_tag="LP543_001034"
     CDS             complement(11622..12566)
                     /locus_tag="LP543_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2321211.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            12787..14001
                     /locus_tag="LP543_001035"
     CDS             12787..14001
                     /locus_tag="LP543_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2321212.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14023..16038
                     /locus_tag="LP543_001036"
     CDS             14023..16038
                     /locus_tag="LP543_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBL2321213.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16121..17494)
                     /locus_tag="LP543_001037"
     CDS             complement(16121..17494)
                     /locus_tag="LP543_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBL2321214.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(17520..17804)
                     /locus_tag="LP543_001038"
     CDS             complement(17520..17804)
                     /locus_tag="LP543_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2321215.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(17806..19821)
                     /locus_tag="LP543_001039"
     CDS             complement(17806..19821)
                     /locus_tag="LP543_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL2321216.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20000..20305)
                     /locus_tag="LP543_001040"
     CDS             complement(20000..20305)
                     /locus_tag="LP543_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBL2321217.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(20394..21425)
                     /locus_tag="LP543_001041"
     CDS             complement(20394..21425)
                     /locus_tag="LP543_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL2321218.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            21632..22507
                     /locus_tag="LP543_001042"
     CDS             21632..22507
                     /locus_tag="LP543_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL2321219.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(22659..23501)
                     /locus_tag="LP543_001043"
     CDS             complement(22659..23501)
                     /locus_tag="LP543_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL2321220.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(23593..25470)
                     /locus_tag="LP543_001044"
     CDS             complement(23593..25470)
                     /locus_tag="LP543_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL2321221.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(25467..26888)
                     /locus_tag="LP543_001045"
     CDS             complement(25467..26888)
                     /locus_tag="LP543_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL2321222.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(26891..30280)
                     /locus_tag="LP543_001046"
     CDS             complement(26891..30280)
                     /locus_tag="LP543_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBL2321223.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            30537..31928
                     /locus_tag="LP543_001047"
     CDS             30537..31928
                     /locus_tag="LP543_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2321224.1"
                     /translation="MEKIIRILSISDKWMIKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32001..32648)
                     /locus_tag="LP543_001048"
     CDS             complement(32001..32648)
                     /locus_tag="LP543_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBL2321225.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(32656..33342)
                     /locus_tag="LP543_001049"
     CDS             complement(32656..33342)
                     /locus_tag="LP543_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2321226.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(33348..34421)
                     /locus_tag="LP543_001050"
     CDS             complement(33348..34421)
                     /locus_tag="LP543_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2321227.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            34643..35317
                     /locus_tag="LP543_001051"
     CDS             34643..35317
                     /locus_tag="LP543_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2321228.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            35322..36662
                     /locus_tag="LP543_001052"
     CDS             35322..36662
                     /locus_tag="LP543_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL2321229.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(36811..37797)
                     /locus_tag="LP543_001053"
     CDS             complement(36811..37797)
                     /locus_tag="LP543_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBL2321230.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            38521..39552
                     /locus_tag="LP543_001054"
     CDS             38521..39552
                     /locus_tag="LP543_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL2321231.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            39661..>40085
                     /locus_tag="LP543_001055"
     CDS             39661..>40085
                     /locus_tag="LP543_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBL2321232.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
BASE COUNT        12416 a         7300 c         7507 g        12862 t
ORIGIN      
        1 ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact taaatcaatt
       61 cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag aaaacaaaaa
      121 aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc atagatacaa
      181 ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt gactgccgca
      241 tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc cattgtcttt
      301 ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt tgcaggagga
      361 aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt tatttagcag
      421 gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg aaccagacaa
      481 aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta gatgagattt
      541 atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa aatcttgaga
      601 tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt caacagctag
      661 tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa tcattagcaa
      721 acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg ggggctggat
      781 tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat cagccgcctg
      841 ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa gtcggaatct
      901 tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa gaagctgcaa
      961 aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag attgcacaaa
     1021 cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat aataccattg
     1081 ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc gtcattccat
     1141 ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat caatatcaat
     1201 tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa aaaccggaaa
     1261 cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct caagctgatc
     1321 gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt acagatgact
     1381 cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt cacaaggcat
     1441 gctttgggca atattaggat tagggatttt catgacttat cggatcttag actttcctga
     1501 tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag ctattactca
     1561 tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga tagcaggact
     1621 cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag ggattttagt
     1681 gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc tttctttatt
     1741 gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt actttgacac
     1801 agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat tcttcttcaa
     1861 tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg ctcggtcgtt
     1921 agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg gtgtcattgc
     1981 attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata aagggattgg
     2041 cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc aagaattgac
     2101 gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc tattgatttt
     2161 agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg ctatcatctt
     2221 agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa cgggttttga
     2281 gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa agtcgtaaac
     2341 aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat cagagaaggg
     2401 gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt caatacgatc
     2461 gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct gatcacaaaa
     2521 gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc caagatggga
     2581 acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag aggtcaaaaa
     2641 cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag actatgtcaa
     2701 gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt atctggtggt
     2761 caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt actattatta
     2821 gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg attaacaaat
     2881 cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat ggaagacgcc
     2941 ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca agatatttct
     3001 gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga agaagtagtg
     3061 gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca ttgacatctt
     3121 cctttgattc atcctatact taaaacaata aacaattaaa catatttggg tgttttgtag
     3181 gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg acaagtaaac
     3241 gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc gtatcaaacg
     3301 ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt tctggacaaa
     3361 ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca ttgatgcaca
     3421 atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa gtaaatccaa
     3481 tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt gtaaatcttt
     3541 cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag gaagcgcaat
     3601 tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc tataatactg
     3661 atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa aagaaaaaaa
     3721 tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagca gcgttagacg
     3781 gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa gtaaaagaaa
     3841 cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac tttgccgata
     3901 cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca acagatatcg
     3961 ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg catgagaatc
     4021 tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa gaagcaaaaa
     4081 aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga gcaatcgcgt
     4141 ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg tttgattaaa
     4201 aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa aagtaatcct
     4261 ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa tgataggctt
     4321 ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga accacaatga
     4381 gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag cgggatacgg
     4441 ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag gaagatgcta
     4501 cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac gagcaacaga
     4561 tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt ttcaatcggc
     4621 aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa atcattctat
     4681 cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt aaatatgtag
     4741 ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg caaaaagcga
     4801 cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa gatgccgggc
     4861 gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg aaactgattt
     4921 cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag atcaattcag
     4981 cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat tttggaatcg
     5041 attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc gatgccttat
     5101 tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt gtagatatta
     5161 aaaaaggggg ccagaagatg gatggacatg ttcttttgca gtacgcacgt ttccgaatgg
     5221 atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac gctattttca
     5281 gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt aaagctttag
     5341 gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt tcgattgcac
     5401 gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg aattatgggc
     5461 aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa tctgctatta
     5521 cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca aggcttttgt
     5581 catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga tttaaccaag
     5641 agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc gtgaagccat
     5701 cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat acggtgaaat
     5761 gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca cgaatgcacg
     5821 gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa ttggtcctga
     5881 attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag aagaaacgaa
     5941 gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg atgctgttcc
     6001 gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc agattaacca
     6061 agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg gatcatcgtt
     6121 tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt tattgccaga
     6181 tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg aggtcagaag
     6241 atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc cgaaaccttc
     6301 caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt tgctggtgaa
     6361 gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg agcttatgaa
     6421 gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac cttccgttac
     6481 gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt gcagtcagcc
     6541 gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc gtggtcgatt
     6601 attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg gatcaattct
     6661 cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa gaactatttt
     6721 ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg aatgaatgga
     6781 agtccattat atagcttgct acttcaacga aaagggattg atggcagttt ttctcttggt
     6841 cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat caaacacttc
     6901 aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg gcagttttct
     6961 ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa ggaaatagaa
     7021 aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac gaaaaaggaa
     7081 aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa gatgaatcag
     7141 ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta cgaaagcaag
     7201 tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca tcagtatctg
     7261 gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca gacaactgag
     7321 atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca tgcaattatt
     7381 ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt acagcaagca
     7441 atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta tatctacgaa
     7501 gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg aaagatccca
     7561 ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga agctcagact
     7621 cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat ggagaaagaa
     7681 acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa aactgcggga
     7741 aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg aatagggaca
     7801 gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat cgaagtcaat
     7861 aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt agccaatgaa
     7921 ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag aaaaattggt
     7981 aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat cctacattta
     8041 ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat tagcgggaca
     8101 cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc aaaatgtaaa
     8161 aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga atgcgacttc
     8221 aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag agatttgctt
     8281 tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg gaaattcgat
     8341 gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg gatcgaaaac
     8401 agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa tgaaaacgaa
     8461 gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg agaatataaa
     8521 taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta ggctgatttt
     8581 atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta aattatctta
     8641 tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct gattacggga
     8701 cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg attttatcga
     8761 ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca agcatgctaa
     8821 agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc attatttatt
     8881 ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac gagagtttgt
     8941 gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa aagcaaaata
     9001 aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact atagaatgag
     9061 aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt agaaaatctg
     9121 gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt gtgcagacag
     9181 gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc aaagcacaag
     9241 aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa gacgcgttaa
     9301 aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa tcaacgcgaa
     9361 agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa atgcattaga
     9421 tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc ctgaaatcta
     9481 tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc ataatggcaa
     9541 taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt gtccagttgc
     9601 tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt cattggattt
     9661 cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa aatgaacgaa
     9721 ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct gattttttta
     9781 tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat taattttgaa
     9841 ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt ttagaactag
     9901 tgggctgatt ttttatatat tttagtttac ataatatata ttatacaaag tagatttaaa
     9961 aaatgataaa cctctctcga acggtttttt atcaaaaatc gtcatttttc catcaaaatc
    10021 tgcttatgca aaattataaa aaaattttgc agtttttctt actttttttg tcgtttaatt
    10081 gaatgataaa aaccagtttc tttattcagt taatcaaata tgaacgaatt agatatgaat
    10141 cattgtattt gccgttgcgg aaggattttt taattaacca cagataaaca ttgataacga
    10201 ttcgcttatt gtaatcaaca gattaaattt catatgttca tatgtacgga tacattttcg
    10261 gaccgattaa ttttaaccat tctgtttatt tgcttaccta ttagatatca ataaaaaact
    10321 gcttaacagc tatttcagct gttaagcagt tttaggattt ttagttcata ttcattgttt
    10381 cttataatac agtgttcaat acttctctta aagcacttgt tgatttgctg aacattgctt
    10441 tttcttcttc gttgattgat aattcaacaa cttcacgaac accattttcg tcaacgattg
    10501 atggcacacc tgtaaagata tctttttcgt cgtattcacc agttaaataa gcagatacag
    10561 gcaatacagc ttgttcgtta ttcaagatag cttttacgat acgtgcagta ctcataccaa
    10621 taccatagta agttgctttt ttacgatcaa tgatctcata agcagcatta cggactttat
    10681 cagcaatcac atctaattca tcttgggcaa tacgatggtc tttttctaca atttcaaaca
    10741 ctggtttacc accgacagtt gtatgtgacc atgcagcaac ttctgaatca ccatgttcac
    10801 ctaagatata gccgtggacg ctgcgtgggt caacttttaa tttcaatgcg atttctttac
    10861 ggaaacgagt tgtatccaat gttgttccag taccgataac acgtgatgtt ggcagaccag
    10921 attcgttcca agcgatatat gtcaaaatat ctactgggtt tgaagcaaca acgataatac
    10981 cgtcgaatcc tgatcccatg atttctttta cgatttggcg catgattgac gcatttgttt
    11041 taactaaatc taaacgagat tgaccaggtt tttggttgat accagctgtg ataacaacga
    11101 tatcagcatc tttacattct tcatagccac ctgaccatac agccacattt tcatcgcccc
    11161 aagccatacc atctaaaagg tccaatgctt ccccttctgc tttttcttga ttcacatcga
    11221 tcaatactaa ttcattagag attccttggt tgatcattgc ataagcgata cttgttccaa
    11281 caaaacctgt accgacgatt actactttac gacttgtttt tttcatgaat aaacagctcc
    11341 ttttcataac tatttgttac tttcatgaaa agtatagtct tgtttattca ttttatcaac
    11401 atgttaatta taatagttgt tattataatt tttgatggaa acgaattcct gaatacgcat
    11461 aacagataaa tgacaaatgt ttacacctat atcccctgat aatacaacca aaagtttaga
    11521 actgaaaata aatccaaaaa aattcgctgc aacgatataa taagacagca atcgcaccta
    11581 aaacaatttt ccttcatatg ctttagtgaa agattttctt cttaatgttt atttctccat
    11641 tcattgggag tacatgttga atacttcttg aataatttga ataaagaagt cgtttgttta
    11701 tatccgatac gattagcgat ttcttccaac gaaaaactcg tttcttgaat cagttccaac
    11761 gcaaagtcga attttttctt tagaaccagt tcttgaaagg aagcatcaaa atacattttc
    11821 aatttattac taagatagac cggattgtag ccaaaatgag ctgctgtttc ttttaacgta
    11881 caagaagtaa aatgcttatc gatataatct aatactctaa gcatctcatt ttctgcgcat
    11941 ccatctgctg atgaaaagct ttgttgttct aataaatgag tcaattcgat cagtaatagt
    12001 gatgtataga gatttaacga acggatctta tgactgtatg tatcaaaata aatcaacagc
    12061 atattttcaa tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg aatgaattgt
    12121 gtatgttccc ctttttcttt tgatgcattt atcaaaaaag aagaaagtag attatttgtg
    12181 cttgccaaat gattcaatat ctctgtagat aaggtttctt cttttaataa gatgttgatt
    12241 aaaatatcct cttgccctgt gtattcgatt ctttgagtag cttctttatc aatgatcacc
    12301 acgtcacttt cttccatctc tatcctttct ccattaataa tttgtaaaca tgtaccgcta
    12361 tacatataat tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc atatcgacgg
    12421 tgtttactga tatgaatatt tccctgttga aatagatccc ctcggtggat ttttggcggt
    12481 gtctgaagat ggtaagctaa gtttaaatca ttgatattca ttccttgttc acgctgcaga
    12541 gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt ccaattgact
    12601 ccctcctttt tgtaagcggt ttctttcgct tattagttca cgctgtactg ctattatatg
    12661 caaagctatt tttggtttca taaagacttt ctatggttgg gacatgaact tcctgtgatt
    12721 ccgctattga gagaataacc acttcatact atatttttga taagcgctta caaaaatgaa
    12781 aggaacatgc ttatgaataa aaagatgatg aaattttctc tgctgtcagt ctctcttatt
    12841 ttgacttctg ctggtgcagt cacgtctgca attcccagta tgtcagctac ttatgccacg
    12901 cgttctttat ctgctgttga attattaacg actgctccct cttttatggt cactattttt
    12961 gttttgatta gtagttttat tgctaggaaa atcggacaaa aacaaactgt gatcatcgga
    13021 ttagtgattg catcgatttg tggtatcctc cccctcttct cacaaagtta cagtattgtc
    13081 ttagcttcaa gagctggact aggtattggc tttggattga tcaattcttt ggcagtcagc
    13141 atgattgctg ccttttatca cggagatgaa cgagcctcta tgattggttt tcaaagtgca
    13201 tttcaaggat ttggtgcagc tttaatgaca tttgttgcag gacagctagt acagttcggt
    13261 tggcaatata cctttttagt ctacgccatc agcttaccta ttcttgtctt gtttgttttg
    13321 tttgttccta cacctgaaac aactgaactt caatcgcatg gcacagcccc aacaagacaa
    13381 gtaatcaaaa cgaatcctaa aatgattagt tatggtattt ttctcttgct ggtcatcgtg
    13441 gtatataatg ctgtatcgat taaattagcc acagtcctga cgacttattc tctaggaaac
    13501 gaaactaatg cagcgaatat cttaacaatc atgcaattgg caagtatggc tgccggagtt
    13561 tgttttggct ggcttcagac aaaagcacat cgctatatgc ttacactttc tttactactg
    13621 atgggaatcg gctttgtatt gattgccact gtgataaacc tgtatgtgat tgggtttggt
    13681 gcgatcctaa caggaatttc tttttcgtta ttcattcctt acttattcaa ccaagtaagt
    13741 atccaagcgc ccaagtacgc ggagagtttc aatacttcaa tcttattggt tggcgctaat
    13801 ttaggtactt tcattgcacc atatggtttg ctctttctct cttttgcttc tcttgggagt
    13861 aaaacacttg ctgttttcgt caatggcagt atcttgctgt tgattagcgc gcttattagc
    13921 cttctagtag tacacaggac gaatagaaag acagtgataa gagtagatga agaaataaaa
    13981 gaacaattga caacaaaata aatttgatgg aggtcgtgta aaatgacaaa tgaaaaacat
    14041 aaagatagat ggctttggta tcctggagac tttgagatcc gccatggact gctacaaaat
    14101 ttccaacggg aagaacgagg ttttgactgg ccagcgtact ggtacatgga tgattgccat
    14161 cggaacgtca agtttaagag atattacttt cttgatcaac cttccatgtt taaagtgacg
    14221 atccaaggag ttgggtatgt agaaattaat ggacaaaaac atccatgtgg aaaatggctg
    14281 acttgtccag ctggtaaagc aaaaattcgt atctttgtag gacatacatc agggctacca
    14341 gcgatgttta tcgagggaga cgaagtaaaa tcagatattg gctggacagc aagtaatttt
    14401 attgaagaat atccagctgg ctggagtccg ctttatgtcg atatcgcaaa agatccaaat
    14461 caaatctatt atcaaaaaga atgcgtccat ccagtcactg aaacggagat tggcggagga
    14521 atcttattcg attttgggcg agcagtgaat ggctgtgtag aactaatatc tctggatgac
    14581 catatcgaat ctatcactct atgttatgga gagtccgatg tagaagctat ggatatcgat
    14641 tattgctact ataagcaaat aggaatgaca agaaaaatga aagctcgtaa acgcgctttt
    14701 cgctatttgt ttataccaga ggtttctact ggaatggttc aacttcaagt ttttcatgaa
    14761 tttttatctc tttcgaaaca agctgctttt tcaagcgatt cttctttact gaatcagatc
    14821 tggtcagtat cagaagaagc cttcaagtta tgtagtgatt tgttttttat cgacggtatc
    14881 aaacgagatc gctggatttg gtctggagat gcgtatcaat cattcatgat caatcaatat
    14941 cttttttttg acgaggaaat caataaacga acgatattag cattaagagg acaaaatgaa
    15001 atcaagcagc atatgaatac tatcgttgac tattcgatct tgtggttgat tggtatcgaa
    15061 aaccactata tgatgacgaa agactatgaa tttttacgca tcgtctaccc taagatgcaa
    15121 tcattaatga gatacttgat ggagcaaacg aatgaattag ggtttattta tgggagagca
    15181 aaagattgga tatttattga ttggtctgaa atggataaag aaggaacgct tgctgcagaa
    15241 caagtcttat tactgaaagc ctatcattcc atgatcactt gcggagatat cttagctgaa
    15301 acatatggag agaacgaaaa ttatcatact catctctatc aagaaaaata tcaactattg
    15361 aaacaaaacc tatttgacta cttttgggat gaagaacttg gcgcttttat cgattgctat
    15421 gaatctggga gaagaaatgt gacgcgtcat gcgaatattt tcgctgtttt attcgatttt
    15481 gctaacaaag accaacagca atcgatctta aagaatgtct tattgaatga cactatcaca
    15541 cagattacta ctccatattt caaattctat gaacaagatg ctttatgcaa actcggacag
    15601 acgaatatcg tctaccaaac gattcttgat tattggggag ggatgttatc tcatgatgcg
    15661 gtaactttct gggaagaata cgaccctact caaacaggaa atgaaaagta cagtatgtat
    15721 ggagatcctt atggaaaaag cttatgccat gcatggggag caagcccaat ctatctttta
    15781 ggacgctatt ttatcggttt gtatccaaca aagccaggtt atgaagaatt tatgatacaa
    15841 cctaatctag attcatttga aacactgaat tgtacgctac ctatcaaaga tggaaagatt
    15901 tcgcttgaaa aaacaaaaga taagatcgtg attacttctg atagaacagg cggaacagtg
    15961 aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc cacttgtctt aacaagctat
    16021 tcaaaaataa atgcataaaa caaaaaactg cttgcgacat atcagaagac tttaaatctt
    16081 cttctgatat tgtccaagca atttttattt tgaagtttgc ttaattctgt tctttagaaa
    16141 caatgtttgt tctttttccg gcaaaaagaa agctagataa tacgacaagt aaaaagaaag
    16201 cgatgattcc aactgtcaag gctatttgac cgtattctcc tagtttccca agtacaagtc
    16261 ctggtactag ataatcagta tcggcaaatg tagaagaagc gattttaaga tctcctaata
    16321 ctggaaggat aaataatggc agccaactaa tcaataagct attgaaagca gaacctaata
    16381 ccgcccctct tctaccgcca gtcgagttgc cgaagacacc agaagcagct ccgcagaaga
    16441 aatgtcctac taccccagga atgataactg tagtttgcaa gcctatcata gcaaacatgg
    16501 atagtgttcc tacaaagaag ctaacaaaaa agccaattaa tacagcatta ggtgcatatg
    16561 gaaaaacgat aggaacatct aatgctggtt tagaatcggg tacgagtttt ttagcaatcc
    16621 cttggaatgc tggaacgatt tctgccaaca ccattcgaac cccttgaagg acgataacaa
    16681 acccagctgt gaatgttcca gcttggatca gtgcaaagat tacagcgttg tctccattgc
    16741 ttagttctct ttcaacaaat ccaggaccag ctaacaaagc aagtgcgaca tatacgatcg
    16801 acatcaagag catgatcgat acagtagaat ctcgcaaaaa gctgaaactt ttaggaatct
    16861 ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt tccgatccat ccactagaag
    16921 cataaccgat atttccggta tgccctaacg caacttgatt gtttccagtg atgttcttca
    16981 taaacggttg acaaagagca ggcgtaatag ttaatagtgt tccttcgaag accccgccta
    17041 ataagatcat ccacaaattt gcagtaaacc ccgcgcttac taaaatgact gctgttagac
    17101 aagaaacata aagcattgct tgacctgtaa gaaaaatgaa tttatatctt gtgaatctag
    17161 caagtaaaat attcacgatc atccctacta gcatgatcaa agcagtaggt gtaccgtatt
    17221 gttgcaaagc aatcgccact actgcttcat tgctaggaac tactccttga gtatgcagag
    17281 ctgtttggaa catcgaagca aaaggggtta gtgaattact gattaaacca gcaccgccga
    17341 tcagtactag gaatccgaca aatgttttga tagttccttg gatcacttct gaaaatttct
    17401 tcttctgaag aacgagacca agcagtgcaa tcaaagaaac gaggatggag ggctgactga
    17461 cgattgacac aaacaaattc aacaatttat ccattgatag ttcctccctt gaatcatttt
    17521 tattcttttg acaagatctt cgttactttt tcttttactt cttcgctgtc aatgatcgat
    17581 tgcaaaggta cgagcttttc atcaggtata gaagttagcg tatctttcaa agaatgctca
    17641 atgaagaaat aatcagccat atcgctagat acacttccaa catccgtatg acttacttca
    17701 aactcgtcca catctgctcc aagatctgca agtgcttgtt tgatgttcat ctctaccata
    17761 aaacttgttc ctaatccact ttgacatact gcaaaaattt tcatatcatt cgtctccttt
    17821 tcttatgatc gctaatattt cttcttttgt tgatgcgtgt aataaatctt gaagattttg
    17881 tttattagaa agtagctttg ttaaatttgc caaagcccga agatgtgcct cattatcgat
    17941 agcagctaaa cagatgaaca aactaatggg atgggctgga ttatcaagca gatccacagg
    18001 ttgttgaagt ttcagcaaag acatccctac ttcgtttacg ccatcttctg gacgagcatg
    18061 aggtaatgcg atgtgatcgc caatatggat aaacgcacca tactgctcca cgcgctcgat
    18121 catagctttg atataacttt cttctatttt attttctgat aatagcggct gtgcggcaaa
    18181 tgtgattgct tcttgccagt tttttggttg atccgataat tggatcattt cttctgttaa
    18241 taattcagat agcataggac gttgatcctc cttcttttct aaaagtttgt tcattttacg
    18301 atttaatact tgatagagct tttcttcgtc tacgccttct tttaattcga tataaggcag
    18361 tagcgccgac aacaattctt gggcagaagg cattgaaaat cctggtatca gccaatctct
    18421 ttgtacttga ttgatcagct gatttttttc caagtcagtc atgattggat gcagaacata
    18481 gagagacttt ttcttatcag taagtgtgag aggtacagta gaaaaaataa tatcgtaaga
    18541 agtttcgtca acctcgtgta agcggtcagc tgaattagtc tctgtaaaaa agatcatcgg
    18601 gaataattgc tgaagttcac tttttaagat caatgaagag ctaatgccac ttggacagac
    18661 gatcacggct cttatttttc tatttctttc ttctgcgtcc gcttttctga tctctccgcc
    18721 aaatagtata gtgaaaaaac caatctcttc ttcagggatg ctcttcccta ttcgtctttc
    18781 taacggaagt aatgcttttc ttgtcatttc gtagatggaa gcatattgat gcttaatttg
    18841 atcgatcatc acattaggta aataaaaaga ataggaaata cgaaaatagg cagggaccaa
    18901 gtgataaaag acattcatca ataattcacg aaacgaagca aattctacgg ccatcaggaa
    18961 ttccatgcga tggatgactt ctgaagaaca ttccagcaaa aaatcaagtg caggctcctg
    19021 gacatctcct tcaacgatcg atgctaagca aagagagaaa aatataatct ccgaattttg
    19081 atgttcggaa tagccaaggc cagtcaatac ttgtcgacta atgtcagtga ctgcaagaga
    19141 agccaacagt tcagcatctt ttaccagttc catggatgtt tttccaattc gaatggttgt
    19201 caaactcaaa taataagcta attcatccat tcgatttggt gcgaatcgaa gatctgatag
    19261 tgaaatcgtt ttatctagca aatctctaac tttggcgtaa taatcaatgg aaagggttgc
    19321 ggttaaatac gtgagcccaa atcttccagc aagtgactgc atcaaacgag cagtgcgatt
    19381 ttttgcttct cttcttaata ttgcttctga ccctagcagc ctaaaaccag ttttcctagt
    19441 atacgacagc tgacaatctt ccacttctaa ttcatttctt atctttttga tatctgacag
    19501 gactgtccct ttagaaacct gaagaaaatc ttgaaaatga aaaacagaaa cagacgttgc
    19561 agtcgaataa accatcaaat aaagaagatc tcttcgctct tgttcggtat acacagcttc
    19621 aaatcgagta atatccaaaa atttttctgc ccagtcgatt gctcggaatt tctttatacg
    19681 gatacgggta tcatccacat attcggcaaa aggagttaat tgtttgatcg cctcgcttgt
    19741 atttacttcg atgatctctg ctgctttttc tagactgatt tctcttggtg ttttcaggag
    19801 ataactaatt ttgtagttca cagcgctgtt cacctcctac ttatattcta gtatggtagc
    19861 gcttttaaat cgaggggtgt ttggtacaac ttacagttgg atttttttta cgactgtacc
    19921 gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg accaaatgtc tcctgctttt
    19981 atgaattgat ttcgtccgat caatgatgat catgtttatt ttttgaggtc agttcttcaa
    20041 atttttcttt gacatcttca accgcttttt cgatatcttt ttcaaagtga ttgggctcat
    20101 taggctttgt ttcctcttct acagaatttg aaccagataa agctgtttct ttattttctg
    20161 tttcaccaga gtttgtgcca gctaatgatt cttctttgtc ttctgtttca ccagaattag
    20221 ttcctgctaa agccacttct tcttctgttt taggggccat tgaacctctt aatcgtttgt
    20281 gtttttcacc gtcataattt gtcatgttgg ttcctcctag tagttctata ctttttcatc
    20341 gtacaataag acagtttatg cgtcaaaaaa caaacccgat tcttacaaat atgtcatatt
    20401 tttccttctg attcttgaat gcttttgatt ccttcttcca aaattttcat ttgtaagatt
    20461 gtttcttcat ctttgatgac tttttctgcg ccattaacta atgtctcgta aatcccttcg
    20521 tagacacggc tgtaatcacc aacctcggaa attacttttt cttcatgata actcccttgt
    20581 tcatctatat aggtcaatac accataatgt tcgggcaaat cgataccaaa gtcttcatga
    20641 tcaggcatgt aaaacatttt cagatgttct tcttgtcgat cttttgtttg tttcacgaac
    20701 atcccttttt ttccgtaaac gacgaaactt ggacgttctt tgatgcggaa ataactggat
    20761 tttatcgaaa ttttcatatt tccatagaaa aaatccagat cgaagtaatc attcattcgt
    20821 cctttcccta aaagttgacg tacatcatag ctgatatcat ctggttcgcc gaaataagaa
    20881 agaacttgat ctactgtatg acaagcatgt ccatataaat agctgttcgc agtagaaaat
    20941 tcatgcgtat tttctggtac ttccggtctg aaataatcat aatgcatttc tacttctaaa
    21001 atatccccta attttccgct ttcgattact ttttggactg tcaagaagtc agaatcgaac
    21061 cgtctgtttt ggtaacattg gataaataga cctttttctt ttgctaatgc aaataactct
    21121 tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta gtacattctt accatgttcg
    21181 agtgccattt ttccatattc ataatgaaat tgactaggtg tagtgatgac aactaattgg
    21241 atctcaggat catcccaaag atcagacagc tgatcagtat attcgatgtc ttcgaatgtt
    21301 ttccacactg gttcagatgg ggaaaaaatt tttttgatat tgattttgtt cgtaagtttt
    21361 cttgaaaaag gcaaatgata gcgatttgta ctctttccat tgccaagata agcgattgta
    21421 agcatgagtg gtgcctcctt ttattgatgt tttcagtata tcctagaaaa gcgcagaaag
    21481 tatttataat agctattctg gtaaaaaagt tcagaaaaac acttgttatc attcggtttg
    21541 gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga tagtagcgga accaagtgtt
    21601 tatcagtaag atagaatttg gaggtcagat catgcttatt gaagaaaaac taacaaaaca
    21661 agagttgttt accacgacag agaaacggat tgtcgattac atccgtagga atatcgaagc
    21721 tgcagtctac atgacgattg aagaattagc aaaagctacg tatacttccc attcagctat
    21781 cattcgttta tgcaaaaaaa tgggattcag cggatttaaa gaattccgtt tcgagctggg
    21841 gcgggaagtt catcaattga ttgctcagtt caatcaaacg attgatccta attttccttt
    21901 ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg gcggatttat cgatccagtc
    21961 aataaaaaaa gcacaattgc agattgaaca tgaagattta gatcaaatcg gtaaagtatt
    22021 gaacaaggca cagcgaatct ttctttttgc aaaaggtgat tctcaaatca cggcaaggaa
    22081 attccagaat aaaatggtca agttgaataa gtttttgatc atggctgaag aatacagtga
    22141 ttcttcttgg aatgcagcca atttgactag cgaggattgc gccattttta ttagctacag
    22201 cggtcgcatc catcattatg aacgaataat gacctatttg aagcatgtag aggcccctac
    22261 cttgctgatt accggaaatc agcattcaga aatggccaaa caggctgata tgtgtcttgt
    22321 tatatcgcag gaggaatatg attttgctaa agtagcgact ttctcctctc aaattgcctt
    22381 cgattacgta ttaaatacgt tattctcagt gatatattct caaaattttg aagagaatat
    22441 ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc cttcttaaag aatatcggaa
    22501 aacttaagaa aagatcctca tttagtaata agaaagatag tgtttgatag gctctctttg
    22561 atatcatcat aaaaacaggc tgaagttttc tttttcaaac ctcagcctgt ttgtttacgg
    22621 aacgattcga atagatatat tatagaaatg aggaatagct aacttctgtt tcttattttg
    22681 aacgattgac gaaaaagata tataagaagg aggaaaatca gtatacatgc agctaacaat
    22741 ccatacagga aaaatcgttt gttctcggat actttctgta cttctttttg aacttctttt
    22801 gcatgataag gaacccggtg ccctctgaca agtaagcgat gactgttgac catataaggt
    22861 gtacaagtta ggagtgtcac gaaatcctgc cctttttcga ttctcagatc ttttgtatca
    22921 gtcggttcta ccgtcttgat ctgatcgact tgataagcga gtgtccgtcc attgatttcg
    22981 atatagaaag aatcacctaa agtcagtttc ggtaagtctg tgaacaactt tgcttgaggc
    23041 agtcctcgat ggctggaaat aacggcatgt gtattttctc cccccgtagg ataagacgtt
    23101 ccttccaata atgaggcacc tttttctagc agcacagtat tcgtttcatc aaaaattggc
    23161 aaatggacat tgatctcagg gatcgtcaaa ataccgatcg tatgtttctc aaaataggtt
    23221 ctatcctttt tcgcaggttc ttttttctta gtaaagggat ctgcacctgg atttccacct
    23281 tcttttgcta attgctggtt tttctttgtc atgttttctt ggattttagc catgacttct
    23341 tcattttctt tgaccgcttg ttgctgataa tgagagatga tctgctgatc cagataatta
    23401 ttcagcgcgt cgctaacaaa tggataagct agtatcccta ttcctgagag aagcaagaaa
    23461 agcatcagca gatctagaag tttccttttc tttggtttca tttgtgttct ccttgtgatg
    23521 actgtttgat aataaaagaa aaaggagcat ttgcgtaatc cgctgctcct ttttccaatt
    23581 cttgcaaaaa gactaaatct gactgtgctt gcgtctagca aagtacagtc cagcaatcag
    23641 tagaaggact actcctgcac cgatatacac atagattccc ttaccgcctg ttgaaggaag
    23701 tgtacctttg tgtttatttg gtattttttc aggagaaatc aactgtcctg ctgttacata
    23761 agattgttca tcgatagtaa atgctacacg gtttgttaat ggaacatatt tttctggcgc
    23821 tttcgtttct tccagatagt acgtgccata ttttagacct gtcacatcga ttaaaccgtt
    23881 acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat acccagctga cggcttttgt
    23941 ggatgggtcg atcgataaat atttcgctgt gtcactatct tgatcacgaa cgacgaattc
    24001 tgctccagca agtgtttggt ctgatgtaac gtcaccatct actttaacaa atcgtttgcc
    24061 cccagtaacg acatcgactg acggtggtgt ttgatcattt gtatgcccgt tatcgacatt
    24121 tgcttggtta gaaaatcctt tggttggatc tgctttttcg ttcaaatgca tatagtaaac
    24181 gaatttcaat gtaccgccag gagttaatga aggaatataa ttcggatcaa ctgaaaccgt
    24241 gaatccgtct gtttgctcag tgacagaata atttactggg tcgatttctt tatttccatc
    24301 atataaggca taagcatacg ttcctgaaga atcattatca aatgttaaag cagcgtcatg
    24361 agtatcgata agtttgaatg ttgtgtactt gttttgcgtt ccttctttat cagcgatacc
    24421 taatgggata ttgacagaaa tctcatattg aattttttca ccgattgcga catcttttcc
    24481 attcaattga ggtgttgttt tatcaacttt agacgtatca tttttgatgg tcttttctac
    24541 tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt gtttgctttt caatcatttc
    24601 cgcattatca ggagctttta cttcttctaa attatatttt ccaacttcta aatgattaac
    24661 gatcaactgg cctttttcaa tagatgcctc ggcaaagtca ttgttgccga tgtcataaga
    24721 atgaccagta atgaaatgtt tggctttggt ttgatcagtt gtccaagtgt acaatccatc
    24781 tgtgacactt tggatgtatt ttttgacgct tggtgttcct tcttctttag aaataataaa
    24841 ttctgctcca tttagtgctt cgttttcggc agtaccgatt tttgtaactt tcaacgttcc
    24901 atcattaccg actgtatttt tagggtagag atggatagta tctagttctt ctgtcccgta
    24961 tttataagag ccatctgctt gtttgatcat ctcatataca gggaaagcta aaaccatgtt
    25021 tgcggcagct gacactccgt cttttggttc ttctttgatc gtgtagactg catctttccc
    25081 attttgtttt ttaggtaaag ataaagtgac atttccatca gcatctgtcg ttcctgaagc
    25141 aactggtgta ccaggagtca aagactggac tgcttgtttt gctgcatcca cggacgctcc
    25201 tttatctcgt tgcgcataaa attcttgagt gacgttataa actgaaaatg aaatatcggc
    25261 taatccttgg tattgatcga attcgctcat ttctttcccg ctgttttgga ttaaaggatc
    25321 gggtaaatca gtcattttct ttttatgaag gatgacttga gctgctgtct cctctgcaaa
    25381 cacttttttt gcgccgaagc tgtttgtgag taatggtaaa ataaggaaaa ggactcctaa
    25441 cataacgttt atttttttat gatttttcat tttttcactc ctgttctttt tttgataaac
    25501 aaccagattg caaagcttgc gaataccata ccaacaacgc ctaaaagtgt atttttcgtt
    25561 tcgtttgttt ttggaagaaa tggacggcta ttttcttttt tattgactac tttcaatata
    25621 ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg taaacggaat ttctgtttca
    25681 cttaaaatat aaccagaagg tgcttcgatc tcttttaatc gataacttcc tgttttcaac
    25741 ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt gaagcgcatc tgattcgttc
    25801 tttgtccact tgtatccgtt ggctgtttgc tggaggtaat ttcctgcttt gtttttaata
    25861 aggaatactg cgccagctaa tcgtttatct ggtttatttg tctcgatttt gacaaattgc
    25921 ttactccctg tatatacaga gactttcttt gtactgacac caaaaccaaa atccagtgtt
    25981 ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc ttagctgcat ctgataagaa
    26041 atggtcagtt tttttccagc atatttttcc aacgaaggga tcgaaaataa aacatcaaat
    26101 ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct tttctccatt cgatgaaatc
    26161 gttaaactat taggtaaaaa cgttaatgct tcatccgcac tatctgacaa ttcgaacttt
    26221 tgatagtcaa ggatatttac aggaattgtc gtatgcaagg aataagtgat tttttcacca
    26281 atcgtaaaat catttctagg ctcttctaat accttctcaa aaggtggatc agggtagtcg
    26341 ttttcttcat ttttaggata caaatggata gttgaaagtt tttgattgtt ttgtccatgt
    26401 acaggaagaa caactaccat attctctgcc ttttctttga cgacttctgg tactttggat
    26461 tcaatgaaaa gatacgcttt atctcttttt tcttgatctt tagcggccag agaaaaagaa
    26521 gcgataccat cttcgttgtt aagagttgta gttgttgctt ctgcggtaaa cataccggaa
    26581 gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta ccgttttgcc cttttcccgt
    26641 agatggtaaa aagaatctgt gacatcataa acttggaatg tgacaccatt taatcctcga
    26701 tacgtttgta ataaagcttt ttcttgtccg tcatttggat gatttttcgg cagttgcccg
    26761 tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt ctgtttctgt ttctgttgct
    26821 acctgagtaa aagctggttt aaatagtaac aagaagagac acatactaag ccaaccaagt
    26881 tttttcatat ttagctgccc cctctttctt ttttcaaaaa aagataccaa atagaaaacg
    26941 cacaaccaat catccctact agatagattc ctaaacggcc aattcctccc gtttcaggta
    27001 atggtacttt tgcctgattc gtgatgtcta aagaaatctg gttgtttttg gctcctggtg
    27061 acagaacaga ttcatgatct tgtccatcca cttgaattga cccatcttcg tgtataacta
    27121 tagtaactgg ctcttttaga ccttggtatc cttctggtgt aaaagtttcg gtcagcgtat
    27181 aagttccagg ttttaaattt tcgaatagaa aggtatctgt ttcttgtcca tcttttggcg
    27241 attctaagtc catttctggt ccctgcagtc tgaattttgc tccttttaat gtctgtccgt
    27301 tgtcttcttt tttgtgtacc gttagatcaa acgcttttag tcgattttga tgagtgagcg
    27361 tccatacatt atttgtcatc tcggtatttt ttcccgtgta aatgattttt ccatatttat
    27421 cgatttggaa ttcataattt cccggcgaag tatcatatcc ggcaggacca gctgtttcaa
    27481 cgactcgata tttaccaggt gtgagtgaat caaaactggc aagacctttt tcattcgttg
    27541 tttggctgtc aattggctga taagtaccat ttgcttcttt tttctgtagg gaaaatgctg
    27601 ctcctttaag atttatctct ttgtccgtcc cttgcatcgc atacttacgt actgctacag
    27661 gaacttcaac aaaaggattt tcaatagtca actggatcgt atcatcggaa gtagtgactg
    27721 tttttccatc aatggttacc gtaccatcag aagcgatctt gatttcccaa gtagtctttt
    27781 tgcttaatcc atgcccttct ggagcttttg tttctgtcag cgtataggtc atttcttttt
    27841 gcaatttgac attttctgga agagaatagg ttccgtcgcc atgatctgtt agtaaagtat
    27901 caatagaatc tcctgttaat ttgaaaacag cgccaataag atccttttca cctgtagagg
    27961 aatttttcgt tattttcaag tttaacggtg tgaattgttt agtatttttc catgtcattg
    28021 catctatttg ttgagaatcg tatcctggta caggtaattc tttgattgtt tggtaactga
    28081 atgcttgacc ttgattattg tattgaggta gtgataatat ctcttgataa ctttctccgc
    28141 tatttgcaga taatttgtca atgtctgcac gttcccacgt atttgttgta tctttagctg
    28201 gtttaatgat tcgaatatat ccgtttttcc aagctgcagc atttgtcgta tgttcccgtt
    28261 gaatctcaaa agtaacttga tctggacgat cagctagatt gttgtcaaat tcttcccata
    28321 actttttgat gtgaagactg actccgggag ctttagcaga tggtataccg aattcagcta
    28381 attcattggt atcaatgttt ggctggaaag ttgtcctgcc gttcatttga taccagaaat
    28441 ttggatggaa gtcctcattt tctgtttgga ttctcacttg gtaatggatt tggatttctt
    28501 ggtcttttcc taaatagatc tgattgctct tgatggtatt tccttctatg gaaatagatg
    28561 gagtgaccgt tgtaggactt gtccccacac tcttgactga aagtgtacca ggctgataaa
    28621 tgaatggttc tgcgattgga tcatttattt gtccattgct tacagttgct gagatctgta
    28681 cagctttttt ggctaggtat tcagagatat ctgttgcatg atcagctgat tcatagtaaa
    28741 gatcgccttt ttcatctgat gaaaccattt gtcgcatacg agactctact tctgcttttg
    28801 agagaccagc tgccggatcg ctttgaagtt ggatgccaag accatgtatt tcgattcctc
    28861 gttctttgag tgccatcgct tctccgatgg ttgcgataaa cgtactgtcg attcttctgg
    28921 atagattgtt ttggtcaggt gcatcataga ttcttgagat tagagaagta tttcccggcc
    28981 gatcttgcgt attagaaaac tgagttccgt aataattgct gcttgattgt gcgtgtaccc
    29041 gctgtacttt ataggaaaat gttggtacac catccgtcag caaaacgatc acttttttat
    29101 gtccatttgg aacggatagc atgcttcctg catctcttag tgctttttgt gtaaaagtac
    29161 caccatttgt ccgtgaaggt gtaatggatt ttacttgatt tttcactgaa tcaaatgaac
    29221 ccatctgtac tgcaccgtta ctgtagctat atccttcgct tgagtagccg acatatccca
    29281 tattgatttt gtctgtgata ccgctatctg ctaaagtatc gacaaaacga tcgacaccaa
    29341 tctttacttc accgatccga ttattgtcgt tcatacttcc tgaccagtct acgaccaata
    29401 cgagatcaag aggcgtgata tctttttgta catttccacg agcattcaaa taaacatcaa
    29461 acaatccggg tgtacttgtt tcctttgcat actttcgtat cgcatagtct gcttgattac
    29521 cggttcctcc atattcgata tacgaatggg tccgatcatt aggatctcca ttccaagaat
    29581 ttataccatc ccattgattt gttccgtttt tattgccttg atgatcaaga acatttgtat
    29641 ttcctgtagg ttgccaactg gctttaggat aagtgccctg atcatctgtt gtatattctg
    29701 taggaatcgt ttgattagat ggcacactca cagctggcaa ggataatgta ggcaatgtaa
    29761 atgggctgac agaattttcg ttttcttttg atgattcttc ttctatttcg tctggctttg
    29821 gtactgacac atctgtatct ttcttatttt ctgtctcatc aataatgtca tattctttat
    29881 tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc tggatcgatt ggaaaactca
    29941 gatcgatagt ttgtttagtg tttgatgaga tttccaacgt aattttcccg ctatttacct
    30001 gataagcata gccatctcct tcttttcttt ccacatcact taagtaattt tcgactagac
    30061 caatttcttt ttctatcttt tcagcactgg tgttattcgc atttagcttg atgttcagtg
    30121 ctttttttcc agactgatcc gaaatgactt cagctgaaat tgtttctaaa actgttggat
    30181 catctgtagt gtctgctatt gcagataatg gcacaaagtt tgataaactc aatattatca
    30241 gcatgaaaat aactttcagt ttcttccctg tggtcgtcat ttttcccctc ctttttcttt
    30301 ttttctgttt tgacgtgaat agtgaaaatt attaaaataa acaggcgtta cataatttta
    30361 tttattttaa tatgataatt attatacgaa gggcgctaaa gggaatcaca ccaattttca
    30421 taccaattgg tttcatttat gaagttgatt ccttcgatta atgagcgttt cattgaaaga
    30481 aaagggttga gggagaaaaa catatgatca ggttgttgga acgaaactat agaaagatgg
    30541 aaaaaatcat tcgaattctg tccatttctg acaagtggat gattaaaaaa gaacttgcca
    30601 aacgaattgg tagttcagaa tctacatttg taaggtatat cgaagagatc aaaatccgct
    30661 ggggaacgga tgtaacgatt caatcttcac aaaaattggg gtatcgtctt gaacatttga
    30721 atgtaccagc ttatttaaat gttctgatgg aaatgtcgca aagttctaca aacagtcgtt
    30781 tattaaatga gattattgtc aatccgggta aaacgattga gtattattgt gaaaaaattt
    30841 ccatcagccg gtcttctttt acacgtaagt tgaaacaatg caatcaaatt ttagaaagct
    30901 atttattaaa agtgatcgtt gaccaaggtt ttcagttaac tagccttaaa agtgagctcc
    30961 agttgcgtat ctttgttacc tttttctttt taagttacta cggaagacgc acgttgccat
    31021 ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa gaataattgt cagatgaatt
    31081 tgtttcagga agaaagccca tatgagcaat ctttctttat tatgtatttt gttgtgcatc
    31141 tcatgagaga atcccaagga actccatttg aatggaaagc tggagactct tatgtacagt
    31201 tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa tctcttagga aaattgaagt
    31261 acagcagcta tataaaagta gtgaactatt ttgtcaatac gattttttca gaattttcgg
    31321 agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa tatgtcctac tgttcgtttt
    31381 catccgctga ttgcaaagaa aaaaacgaag aatatgttgt tcaattatta acgcaaattt
    31441 attttttctc ccgtattttc ccatacgata cgactcattt gacgatccgt gcaacttact
    31501 ttgcacagca gatgcaacac cgccagatac gtctatatgc tggatttcgt gcagaacttc
    31561 aattttattc cactctactg aatcagaatc tgatttatta tttatctagc tatatctttt
    31621 ggatgcttat cggtaatcct gaaattcacc attatacttc tgataaaaaa atactgatta
    31681 tcagcgactt aagtcttcga catagtcaat atattgaaga atatatcagt gatattcttg
    31741 ctgtgcataa aattcactct gagacaactg ctattactga agatcagctg tcgaaataca
    31801 atcttctcga gtatgatctg atcgttacga atcaacccat tttgaatgcc catgttcctc
    31861 atatattgat tgatgatagt gtgtcattcg caaatgaaga agaactcatc cgcttattcg
    31921 agttatgaat aatcaaaaaa cagctatgta taaaccaccc tgataaaaat ttcaggacaa
    31981 atacacatag ctgtttcttt ttattgtatt ccttcagcaa tccaacgaga tttatagcga
    32041 aagtctttta acaactcttc ttttgtccaa ttatttgcaa tgagatccgt caaagtgttc
    32101 attacgaggg tcatggaaaa gtataggaaa ctaactgctt cttccgacga ttgaatggag
    32161 aaatgattga ttcttaataa ttccagccat ttttcgttca ttggagaatc agccgatagt
    32221 tctctacgtt tggttgtctg ctgatcattt cgctgggtta acaagtataa cctttgccaa
    32281 tagatacttt cgtgatctaa gtcgcagcac cactctaaat atttttcgat ccccaaaaag
    32341 aaatgttgtt tgttttcgct aatgaatttt aagatatctt gatggactat gtgagaactg
    32401 cttttgatcg tatacaaata agcatcttcc aaatcttgaa aatatttata aaaagcaccg
    32461 cgcgacatcc ccatcttgtc aacgatttct gagactttga cttggctgat gtgctgtgag
    32521 caaaatatct ccagtaatag gtcatcgatt ctctgtttct ttgcgttcga taaattgaaa
    32581 aaagtttctt ttggcatcca catctctcct ttatctctga ttatacgtgg atagcccttc
    32641 attgtcatga aggattcatt ccttcttttt ctcttcttct gtcaagtacc catcttccat
    32701 tcgataaacg cgatcactcc attgaatcat acggacatca tgagtaacca taatagtcgc
    32761 tttttgtttc tcatgagctt ctttaactag taatttgacg acttcatatg catgatctgt
    32821 atccaaacta gctgttggtt cgtctgctaa gatcaagctt ggctcattat ataaagcgcg
    32881 agcaattgcc actctctgtc tttctccacc agaaaggtct ttaggataac tatttttaag
    32941 tgactggata tctaaagatt gcagcaagga atcgattttt gttgaatctt ctttttttga
    33001 agtaacttgg tcaactagtt caaattgttc ctttaccgtt aaaaaaggaa tcaaattaga
    33061 gctttggaga ataaatccta tttctttaaa gcgtaaatct gctcgttttt tttctggaag
    33121 ctcagttagt gagtgaccgt tgatcactat ttctccggaa gtcggtgttt gcaatcctcc
    33181 tgaaatggta aggaaagtac ttttacctga accagaagga ccaatgatgc tgacgaattc
    33241 tccttccttt acataaaaat caattgcttt caaagctgag acctttgtat gaccagaacc
    33301 aaatgttttg gtaacttttt tcatcgttaa tatttttttc atttttatta acctcctatt
    33361 gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa acaatccgcc tagcattgcg
    33421 acaatgatca aaatcccact atatagcaac cattgtgacc aaaaaacagc aaaaggcatc
    33481 gcgcttggta aaatcagact agtcaaataa gcaaacaaaa gagcaagcag aacgcctacc
    33541 accccaacaa taaaggactg tgctactaaa gatttagcga tgaagaaatt tttgatccct
    33601 tgtgctttca ttactccaaa aatagcagtc ttctgaagtg tcatcacgta catgaaaata
    33661 ccgaccacgg cagcaaccac taagaacaaa aagtatatca tcgcatttaa ggtcatattt
    33721 tgggcagaat agccaggaat actttcaatg aatgtcccaa tagttaataa ctgtagaccc
    33781 ttcgatgctt tttctttcga aattttagct gaatccttta ttacgatccc attgatcggc
    33841 tgttcttctt cagaagcaaa cggctgttgt ccatacttca aagcagtcca cgtatctagg
    33901 gacgcataga tgacgggaga aactgtgtaa taagtttcag gaaagatacc gacaatcgtc
    33961 aaaggatcgt cgtaactgcc aattttgatc gtatctccaa ttttatagcc tgaatcagcc
    34021 agattttgag agatgatgat ttcattcttt gttttaaatt catgcccttt cgtgagtttt
    34081 ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa cattttcttt gttttttcct
    34141 ttgattgctc cgctgtacaa tccgattggt gctttctctc ctccttcaat gtatttcaga
    34201 tctcctcttg ttaattgtga agctgcaaat acttgatttg catcctccga caaaacaatc
    34261 tcttgtgcgt cccaatcatc gattgctttt ttaaactctt ctgaaagtcc attcgctagt
    34321 cctgacaaca tgaatacgac atacgcaatc aagaacataa taccgacgat caagccatat
    34381 cgtaatttag aatagcgaat ttctttgata gctagaaaca tttgtttttc ctccctatga
    34441 tagattgata gtgacaaaat gtcacttgtt gaatagtgta actctaaagt gacaatttgt
    34501 cacttgtgtt gtttttttct tatcaataaa ttgtctaaga catctttcga taaaacctta
    34561 acatgagtca taaaaaattt atttaagcct tatttatgct ttccatgata aactaaagtg
    34621 accaaatcaa tgaggtggga aaatgattaa actattagta gtagaagacg ataaagtatt
    34681 atccaataat ataaaagaaa tactcgctgc aatcggcgag ataacacaag tttatgatgg
    34741 tagtgaagga ctgttcgagt tggaaagtgg agtttatgat cttgcaatct tggatttgat
    34801 gatgccggaa atgaatggat atgaagtgtt agcaaaaata cggaaagaaa aaatacagac
    34861 tccagtattg attttgactg caaaagatag tttggaagat aagattgaag gatttgaacg
    34921 aggtgcagat gattatctaa caaaaccttt ctatcgagaa gaactgatca tgcgcgtgaa
    34981 agcattattg aaacgctcgc taggtctctt tgatgaaaat gttctgaagt ataaggatat
    35041 cagttgtgat ttaaaaagca atgaagtctt gtataaagaa caagttttgc ccatacaagg
    35101 aaaagagttt gaattgctcg tttatttttt acaaaataag ggaatcattt tgacaaaaga
    35161 gcagatattt gaccggatct ggggatttga ttctgatacg acgattacag ttattgaagt
    35221 ttatatgagc aacttacgaa aacatctgcg aagtactgga ttggataaag aaatcaaaac
    35281 cttacgtaat gtcggttata tactgcaaag ggtgtgatgc aatgaatgga gatattacac
    35341 gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc atttgctttt atcttttttc
    35401 ttttgggatt cattacttta cagatattaa accaatctgc atacagagaa acagatctgt
    35461 cattagagaa aataaatttt gattctcgaa cagtacaatt agagatacaa agatatcagc
    35521 aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa cagaacaaac atggaacagc
    35581 cattaatgga accatcaaat gaccgtttta acactcaagt gatcttgtgg tctaaaaatg
    35641 gtgagatatt gaataaagca gctattggtg ggcgtttcaa ccaaatcaaa gaattaaaaa
    35701 tggatacgaa aaatctgaat accatccaaa atattgaatt aaatgaagac agtggctcat
    35761 ctgccctctc tttccgttcg attacgaaaa aagcggagac gaatcaagat ggcgttgcct
    35821 acatccaaat tttagaaaat acaaatcaaa tcaagaattc attagcgact tttcgcacga
    35881 ttatgatttt gtgtatgatt gttttttgga ttctctctat tggtatcagt tattacttgt
    35941 ctagtctaag catgcgccct attattcttt cttggagaag acaaaaagaa tttgttgaga
    36001 atgcttctca tgaattgcgc acaccgttaa cgattattca aaatagtttg gaacatcttt
    36061 ttaccaagcc taatcatact attattgaag aatccgaaag catcgctcaa gcattgtcag
    36121 agacgaggcg cctcactgga ttgacttcgg atttgctgac aatcgctcgg aatgattctg
    36181 atcagcagct gctttcaaag caaatgatca atacacaaga atatatcgaa caacttgtta
    36241 aaccatttca agagatggcc atcatagatg gaaaagagtt tattctccat aacgatgcaa
    36301 cattagaagt accaattgat gaaaaaaaaa tccatcaagt tttggttatt ttgctggata
    36361 atgcattaaa atatacgaat ccagaagata agatcatttt ggattcatct gttaaccata
    36421 aagaatggct gattcacgta aagaatactg gcccttctat cagtgaagaa gatcaagcaa
    36481 ggatttttga tcgattttat cgggaagatc aatctcgggc gaaagagact ggcggatacg
    36541 ggcttggctt ggcaattgca aaacaggtaa tcgaacagca tcatggacag atttctgtat
    36601 ccaattatct tccacaagga gtcgatttta aagtccaatt gccgttgaag cctaaaaaat
    36661 aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt cacagccctt ttttgttgcg
    36721 aataaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttct ttaagctgac
    36781 cacttctgtc acaacctctt tagcttacgc ttacgatttt ttattgcggt cttcaatgtg
    36841 tacatcaccg cttgtgtcaa tatccaattc ttctcggcta acatcttctg tcactttttc
    36901 aacatcttca tgttcttctt tatgaatccc aacttcttca cgaataactg tatgtttgct
    36961 tacatctact tgctcttctt tgattgggat agtaatcgtg tcatcttcca atttttcgtc
    37021 tgttccctgt gaagattgat ctgttactgg tttgcgttcg atgacgattt cttcacgttt
    37081 gaccggtact tctaccgttt cagtatcgtt gaccacatgt ttgtggatgt cgacttctcc
    37141 agttgtcaca tcatgtgtat ttacatctaa tttttcttct tttagttgaa ttgtatcatc
    37201 atctagattt ggtttgcgat cgatgtcttc agtcttgctt gtacccattg tatcagtctc
    37261 gccaggtacg tccatcggat cagttcctgg tgtcatattc aatggatccg ttgtattttg
    37321 aaagcctgtc gtgccaccta cgtccattgg atctgttcca gtgccagttt ctaatccagt
    37381 ccccattgtg cttgctgggc taacagtatc ttgtgtatcc attgtatctt ctgttgtttc
    37441 tggctgacga tagcctttta ctgtgatgac aaattttcca tcagaaatat ctcctctata
    37501 ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa tcataggtat caaaagaaaa
    37561 agtatctttt attttttccc ataaagaacg gtcttcttca ccatccatcc gattttcatc
    37621 tgtttcaaca tctataccag acaatccgtc aaatcctagt ctagatgctg cttcatcagt
    37681 agtataaata acgatatcat ctcgttcata cccttcgtta cgcaaccgtt caattactga
    37741 taatgcttct tccctcgtac tatagcttcc gatgaccgtt aaatttttct cttccatgta
    37801 aattcctcct agcaaataga tttatgattg gtatattgtt acttataaat ttattctata
    37861 ggagacgatt gttttctaca aatatttaga ttggaatttg attaaaacaa tttaattgaa
    37921 atttgaaaac atttatgtta atgaaagtca tattctaata aaatgtcatt tgtttctata
    37981 tgaattttta tgaaaaattt catataattt ccactcgttg tgatggttta atttatccaa
    38041 tccatcaata agcaaacaac ttatataaaa aaggattaat tctcaatata attgctccta
    38101 ccatagaata aatcttcatg taaagtaatg aagtaccaac tatttctaat acttctttaa
    38161 gaattatccc aacaatatcg ttagacaaac cattattgaa taaatggaga gccaatcgat
    38221 tctgaaaaaa tgttttctaa tttttcggaa ataaaagaaa gagcctcctg ccattagaac
    38281 gaaccaaatc catttcattg ctcatgaagt aaccacatag ctaaaaagag gataaatcaa
    38341 ttaaaagatc aaaagaatga ctgcactaat aaaataacta aaaatgtatc ttcttacttc
    38401 atatctttta gctagtactt agttaaaaat ttgcttccta agatcaagac aaactgtcta
    38461 tcttcatttg atgaaatgcg gtaaaatata gaaaatgaac actattggag ggaatttgta
    38521 atggacagca aaactagaaa acaactgcaa gaaaagttaa agcaacgtac acacttgctc
    38581 ccttccgtct ttcaacagtt tttagaagaa aatcgttctt ccctttcatt aggcagtcgc
    38641 tatgaatacg caaaagattt tcatctgttt actgattatt tgagaaaagt attttctgaa
    38701 gaagtggatg atcagctgat tatccattta gaaaaacaag attatcagaa tttcttagct
    38761 aaaatctatc gacatactcg ttcgttccaa actacagcga accaagaaac ggagcaattg
    38821 tttgaaaata gctattacgg gatttcaagg aagcaatatt cattaaatca ttttttacga
    38881 tttttgtatc aaaaaggact gattgaagaa gagatttctc ttttgggtac aagtttaccg
    38941 gaaacaacca aagcagctcc gcgatatatc gatgcagaac ttttcaaaga ttttgagcat
    39001 ttgtttatcc cgattgaagg ctttcaaaca agcagagaag ctagctttga agaaaagaat
    39061 agaccgagga accttgcaat tactgccatt cttctgtatt gcggattaaa gatccatgaa
    39121 gtagtagaac ttaaacgtaa agatgtatct ttgaccaagc agattcttaa tctgaataga
    39181 aaagaaaaca gactgaataa agtaccaata ccaaaagaag cgatgccatt tctggagaac
    39241 tattttaagt taacagcaca aaatacggac gaaaattctt ttgtttttcg ttcctcacat
    39301 gatcagcaaa tttctcctcg gactattcgc caaattttga gcaaaattac agtatataaa
    39361 aacgtatcgc cagaactatg cagaaaaaca tatcgttatt atgcagaact atatttagat
    39421 gatgctgacg cggtaaatta tttatgtggt aatcgatacc tctcatccca ttcttttcaa
    39481 gatatctggg aaaagatggt tgatttttct tatcacgaat tagcggcatt cgttcgggtt
    39541 tcttccatat aaaacaaatg tatgatcttt ttttcacaat aaactatgtt attatataaa
    39601 ggattatgtt acgattatct tgaatttgta tattacttaa caaaagataa tggagtgtga
    39661 atgatggaga ccataacgta ttcccctttg aacttattta cgaattttca agaagctgca
    39721 gctaactttt ccgagacccc gattatcttt gatgagccac tttcagcttt cccagaatta
    39781 ggacttgaaa caacttacaa aaaatgctgc gaaagtgtgt tgaaacgggc ttatcaattg
    39841 gctcatcttg gagtgaaatc cggagacaaa gttattattt ataagagttc cgcttttgac
    39901 acataccttc tagctgtttc tgtgtcttac cttggtgcag tgcctgtcat gacttcttat
    39961 catctaccaa caacaacaat ggaagtattt atcgatcgtt tagaagaccc tttcatttta
    40021 tttgatgatg agacaaaaaa gcgagtgaga gaaatttcaa atggaacaaa atcaaaacag
    40081 attcc
//
LOCUS       DAFEPQ010000018        39958 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.018, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000018
VERSION     DAFEPQ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.018"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="LP543_001056"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="LP543_001056"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBL2321233.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="LP543_001057"
     CDS             1011..2258
                     /locus_tag="LP543_001057"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBL2321234.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="LP543_001058"
     CDS             2334..2678
                     /locus_tag="LP543_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321235.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="LP543_001059"
     CDS             complement(2795..3121)
                     /locus_tag="LP543_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321236.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3494..4696)
                     /locus_tag="LP543_001060"
     CDS             complement(3494..4696)
                     /locus_tag="LP543_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2321237.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4813..5328
                     /locus_tag="LP543_001061"
     CDS             4813..5328
                     /locus_tag="LP543_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL2321238.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5527..5973)
                     /locus_tag="LP543_001062"
     CDS             complement(5527..5973)
                     /locus_tag="LP543_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBL2321239.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6020..7498)
                     /gene="msr(C)"
                     /locus_tag="LP543_001063"
     CDS             complement(6020..7498)
                     /gene="msr(C)"
                     /locus_tag="LP543_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBL2321240.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7852..8481)
                     /locus_tag="LP543_001064"
     CDS             complement(7852..8481)
                     /locus_tag="LP543_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL2321241.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8465..8929)
                     /locus_tag="LP543_001065"
     CDS             complement(8465..8929)
                     /locus_tag="LP543_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL2321242.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9416..10684)
                     /locus_tag="LP543_001066"
     CDS             complement(9416..10684)
                     /locus_tag="LP543_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBL2321243.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10915..11652)
                     /locus_tag="LP543_001067"
     CDS             complement(10915..11652)
                     /locus_tag="LP543_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL2321244.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11719..12288)
                     /locus_tag="LP543_001068"
     CDS             complement(11719..12288)
                     /locus_tag="LP543_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBL2321245.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12416..13288
                     /locus_tag="LP543_001069"
     CDS             12416..13288
                     /locus_tag="LP543_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL2321246.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13630..14502
                     /locus_tag="LP543_001070"
     CDS             13630..14502
                     /locus_tag="LP543_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL2321247.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14499..16457
                     /locus_tag="LP543_001071"
     CDS             14499..16457
                     /locus_tag="LP543_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2321248.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16708..17559)
                     /locus_tag="LP543_001072"
     CDS             complement(16708..17559)
                     /locus_tag="LP543_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL2321249.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17682..18149
                     /locus_tag="LP543_001073"
     CDS             17682..18149
                     /locus_tag="LP543_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBL2321250.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18412..18894)
                     /locus_tag="LP543_001074"
     CDS             complement(18412..18894)
                     /locus_tag="LP543_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBL2321251.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18994..19857)
                     /locus_tag="LP543_001075"
     CDS             complement(18994..19857)
                     /locus_tag="LP543_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBL2321252.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            20455..21303
                     /locus_tag="LP543_001076"
     CDS             20455..21303
                     /locus_tag="LP543_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321253.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(21474..21815)
                     /locus_tag="LP543_001077"
     CDS             complement(21474..21815)
                     /locus_tag="LP543_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HBL2321254.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            21901..22608
                     /locus_tag="LP543_001078"
     CDS             21901..22608
                     /locus_tag="LP543_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBL2321255.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            22595..22918
                     /locus_tag="LP543_001079"
     CDS             22595..22918
                     /locus_tag="LP543_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBL2321256.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(23007..24044)
                     /locus_tag="LP543_001080"
     CDS             complement(23007..24044)
                     /locus_tag="LP543_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL2321257.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(24491..25474)
                     /locus_tag="LP543_001081"
     CDS             complement(24491..25474)
                     /locus_tag="LP543_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBL2321258.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(25890..26660)
                     /locus_tag="LP543_001082"
     CDS             complement(25890..26660)
                     /locus_tag="LP543_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2321259.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(26653..27588)
                     /locus_tag="LP543_001083"
     CDS             complement(26653..27588)
                     /locus_tag="LP543_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2321260.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(28182..29831)
                     /locus_tag="LP543_001084"
     CDS             complement(28182..29831)
                     /locus_tag="LP543_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBL2321261.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(30026..30922)
                     /locus_tag="LP543_001085"
     CDS             complement(30026..30922)
                     /locus_tag="LP543_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL2321262.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(30915..31595)
                     /locus_tag="LP543_001086"
     CDS             complement(30915..31595)
                     /locus_tag="LP543_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2321263.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(31596..32378)
                     /locus_tag="LP543_001087"
     CDS             complement(31596..32378)
                     /locus_tag="LP543_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2321264.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(32371..33288)
                     /locus_tag="LP543_001088"
     CDS             complement(32371..33288)
                     /locus_tag="LP543_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2321265.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(33291..33575)
                     /locus_tag="LP543_001089"
     CDS             complement(33291..33575)
                     /locus_tag="LP543_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321266.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            34363..37590
                     /gene="fss3"
                     /locus_tag="LP543_001090"
     CDS             34363..37590
                     /gene="fss3"
                     /locus_tag="LP543_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBL2321267.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            37703..38017
                     /locus_tag="LP543_001091"
     CDS             37703..38017
                     /locus_tag="LP543_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL2321268.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            38030..38404
                     /locus_tag="LP543_001092"
     CDS             38030..38404
                     /locus_tag="LP543_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL2321269.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            38414..38749
                     /locus_tag="LP543_001093"
     CDS             38414..38749
                     /locus_tag="LP543_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HBL2321270.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            38832..>39958
                     /locus_tag="LP543_001094"
     CDS             38832..>39958
                     /locus_tag="LP543_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBL2321271.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRD"
BASE COUNT        12155 a         8142 c         7384 g        12277 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgac cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
     3421 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
     3481 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
     3541 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
     3601 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
     3661 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
     3721 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
     3781 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
     3841 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
     3901 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
     3961 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
     4021 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
     4081 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
     4141 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
     4201 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
     4261 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
     4321 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
     4381 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gtttttttca
     4441 aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg actaacatgt
     4501 aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt
     4561 ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta gctgctacaa
     4621 ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata tctatttgat
     4681 tctccaatat ttccatccct ttctctctat tagcatcata catgatttat tttacatgta
     4741 aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa taagacgaga
     4801 ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt atccaaacca
     4861 cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca tcacagtcag
     4921 atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt ggacaaagac
     4981 gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct tgtagaggaa
     5041 tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc aaaaatctat
     5101 gctttttcga cgctcgataa aacaacagct actggactgc tattcactag agaacaacag
     5161 ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct agacaagaaa
     5221 caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca gcaggaactc
     5281 caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg gataaacaga
     5341 acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc aatacagttg
     5401 gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca ctatttttct
     5461 agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc aaataacagt
     5521 gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg ggccaacgat
     5581 gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca tataagcaga
     5641 gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca tacatgtcac
     5701 cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc tttctggatt
     5761 tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga catccaaaat
     5821 gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat gaagatcttg
     5881 atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca tcattgttgt
     5941 aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta aatttgtcac
     6001 gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt ttctgccctt
     6061 ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga gaagtaaaca
     6121 aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt gccatatcaa
     6181 taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa agcaaagcaa
     6241 tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc aaagtttcct
     6301 gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac tctgtttgtc
     6361 tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt tgctgatata
     6421 cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg atatgttcta
     6481 ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta cctaaagaaa
     6541 actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg acattttgtc
     6601 ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa cggatttgtt
     6661 tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct aatgttttcg
     6721 cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac aaccgatccg
     6781 gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc gcttgttttt
     6841 tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac tctgcttctc
     6901 gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa ttccccgaaa
     6961 actctcggat ttctccgtct tttacttccc agattttcga tgctagctca tctagaaaat
     7021 agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct tcaatcagtc
     7081 gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc ccttgttcat
     7141 aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc catttccgct
     7201 ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt aggtcctttg
     7261 cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt tggattttcc
     7321 catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta ccgacaccat
     7381 tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca atagatagtt
     7441 ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa ttttccatat
     7501 gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag agcagaaaaa
     7561 tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata actatgacta
     7621 aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa atggacatac
     7681 tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg ttattcaaaa
     7741 gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt attaggttac
     7801 cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct tttattcgaa
     7861 cgtagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc
     7921 tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg cctctgccaa
     7981 ctgatctgct tcgaatccac caattggatt tgtttcatag ccatgatcac gcgcaactaa
     8041 catgaattgc atagccgcta agctagaatc gactttgact acatcgttca tttcttctct
     8101 tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga cttcttttgg
     8161 catcttccct gattcgtaag cttggttata gatttcttcg ccatattcgt aacattgcat
     8221 atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat tgaaacggat
     8281 caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc gccatggttg
     8341 catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca tttcttcgtg
     8401 tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca taattgttga
     8461 aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt atattgcaaa
     8521 gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca tcgctgaaag
     8581 ccggggatag cacggaacct gtatctttat ggtccatttc acaatgctcg attttcttct
     8641 gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt ctctgacgcg
     8701 aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct tgatcaatct
     8761 gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt ctcatcatga
     8821 tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg attttttgat
     8881 cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata atttcctcct
     8941 ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa aagaaaaaag
     9001 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
     9061 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
     9121 ttgattctta tcaaactata gaagatagct cttctgcagt agaaaatttt acaccgccaa
     9181 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
     9241 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
     9301 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
     9361 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
     9421 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
     9481 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
     9541 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
     9601 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
     9661 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
     9721 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
     9781 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
     9841 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
     9901 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
     9961 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
    10021 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
    10081 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
    10141 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
    10201 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
    10261 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
    10321 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
    10381 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
    10441 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
    10501 ttgtattttc tgagacagta gctgactgca aagtactctc tgtatcgttt tcaattcctg
    10561 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
    10621 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
    10681 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
    10741 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttgata aatttgcttt
    10801 ttctacataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
    10861 tcgtttgaat aactatttaa caagtaattc tctttggata ttatccgtta tggattatac
    10921 ttcctgtgtc gtcggacgga taatcatgtt actgacagac atccggtctg gcgtttctat
    10981 cgcaaatgct acagcagaag ctatatcttc ggaggtcaat ccccattctt tttgcgcttc
    11041 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
    11101 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
    11161 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
    11221 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
    11281 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
    11341 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
    11401 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
    11461 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
    11521 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
    11581 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
    11641 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
    11701 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
    11761 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat
    11821 ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt
    11881 tttcttgatg tggattggct ttactgacaa gcctcttcgt tcttttggat cggttagatg
    11941 gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc
    12001 acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg
    12061 tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact
    12121 aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc
    12181 tgctaaaggc tcttcgtttt cagcttggat ctgatggatc acttcaaaga gcggatccgt
    12241 tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc
    12301 ttttatcctt actttataac ggacggctta tcatgtctaa tacttataaa acatacttaa
    12361 ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga
    12421 tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc
    12481 aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga
    12541 acttggtgtg acacttttca ctcggggctc ccgagagata cagttgacgg attctggtcg
    12601 atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt
    12661 aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat
    12721 ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca
    12781 tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt
    12841 tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca
    12901 agatcgttgg ggacttttga tacatcgaaa tcatccttta gcaaagcaaa ccgtcatcca
    12961 tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca
    13021 actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt
    13081 attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg
    13141 gatcatcaat acttatggga cggaaaaaat attcatccca ctcgatccgc cattacaagc
    13201 ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt
    13261 agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt
    13321 aacaacgaat aagataagca aaagatccaa cgattaaata acataagttt cttaatttat
    13381 ttcttccttt tatttctgat aattccagta aattactata attataaaaa aagaattgac
    13441 aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag
    13501 atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc
    13561 tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag
    13621 ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt
    13681 ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg
    13741 atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg
    13801 caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat
    13861 catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac
    13921 cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta
    13981 agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat
    14041 ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat
    14101 attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt
    14161 gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca
    14221 ttttcactga attattcaag actggtgaat catttacgac tgcttctaca acgaacccat
    14281 gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca
    14341 gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg
    14401 atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc
    14461 gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa
    14521 tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga
    14581 tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg
    14641 gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt
    14701 cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa
    14761 atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc
    14821 catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag
    14881 ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca
    14941 agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc
    15001 tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag
    15061 gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc
    15121 atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat
    15181 ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa
    15241 tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa
    15301 acaaagcaaa aacagcttcc ttgatgctag cagcagcttt tgcttcattc tttactggtg
    15361 tgacagaacc acttgaattt tcatttatgt tcgttgcttg gccattatat gtcttgcatg
    15421 caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg
    15481 cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc
    15541 cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact
    15601 ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg
    15661 acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg
    15721 ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt
    15781 tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg
    15841 gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg
    15901 ttgcagatga gatggcaaaa ctacatggtg gtgctgcagc acgacctgca caaacaaaca
    15961 ccgtggttaa aacagaaaca tttgccggtg aaacgatcga acaagatatt tacgctatcg
    16021 caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga
    16081 tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca atcgctggta
    16141 cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg
    16201 aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat
    16261 atgtagaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg
    16321 ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag
    16381 tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg
    16441 tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga
    16501 gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca
    16561 actccgcggt aataagtcca gctaaacgaa acatatgaag agctattttt tatttgactc
    16621 ttcttattac ttgatgcaga acagcttttt cacaaaattt ttcttcatac tcaaaactaa
    16681 tcggcttttt cacaacctct ttatacatta ttcctgatcc aatatttctt ttaatgctgc
    16741 ttcaagagaa gttaatggtt ttcccaatac tttttctaaa tcatcagagg aaatagctaa
    16801 ctgatccttt ttaatatcat gttggatcgc taaaaacatg tcagctgctt cttgagggaa
    16861 tccggcttct tttagatttt cgacaaacgc tgcatcactt gcttcaataa tttctaactc
    16921 tttacctgtc gcacgctcta atgccttggc taattcttca tagtcagtta gattgccgga
    16981 taattctaag atttctggag agtcactttc tactagtact tttgctgcag cttctgcata
    17041 ttcacgtttc aatgcccaac ctgtttttcc atttcctgca gcataaacga attgaccagt
    17101 ttttaaagcc tgcccaatga taggtaattc attctctaca taccaattat tccgtaaaaa
    17161 tgtatgggaa atacctgaat ccttaatcat tttttcagtg aattggtgat ctggcgctaa
    17221 tatactgatc gagtgatcgg catctgcaaa acttgtataa gcgatataag ataccccagc
    17281 tgcttgtgct gccttcacta cattcttatg ttcggcttga cgatttcctg gaacgccaga
    17341 gacaaaaagc aatcgatcta ttcctttcaa cgcttctttc attgagtcta aatcatcata
    17401 atcaccaata cgtatattaa atccagcttc tttcagtgct tctccttttt cttcactacg
    17461 tactagtaca tagatttcat tttgtggaac catctttttt aagaattcca gcgcataacc
    17521 accaaaacct ccagttgctc ctgttactaa ataatgcata tttgccactc cctattcttt
    17581 ttatttgtta agatttacag ctgtacattt ataaattaca cctttatcat aaagagtgtg
    17641 gtaagataag tcaagcaaaa aaaatgaaag gatgacaact gatgaagtat tctctgcaat
    17701 ttagtgatgc aatccatgta cttgcctata ttgaaatgtt tcaagatacc cgatcacttt
    17761 ctagtgagat gattgccagt agtatcgaaa caaatgcagc aaatgtacgt aaaatcatga
    17821 gtcagctcaa aaaatccggt ttgatcttta caaaaaccgg aaaaccgcag ccttcccttg
    17881 ccaaaagtcc agatcagatt tctttactag atgtctatcg cagcatcgaa ggtaatacac
    17941 agcttatcca agtcgatcca aaaacaaatc caaattgcgt cgttggtgcg aatatccaag
    18001 aagctttaga agaaagctac gctcggctgc agaaaaaggc agaagaagaa atggctaaga
    18061 tcacacttga tagtctgata cataaaatag ccgttttgga aaaagaaaaa agaccagaaa
    18121 acacaggcct cgtagagaga tttttataat tctctctact gtgcctgcat ttttctgatc
    18181 ggaatcgtct acataaattc ttttattctc cgggggacaa aagccttgtc atgccccttt
    18241 ttcctctcta atgctcaggt caaagcgctg aagtcatgac gtcttgataa acggtgttca
    18301 agtagctgac cctcggcatt agttctgaaa aatgcaaaat atggaaagct atttcccatt
    18361 tttcctctct aatgctcagg tcaaagcgct actttcacga cctctatatt atcatggttt
    18421 attgactacc ggtatccaga tttctcccat atatttcccg ttggtaactc ccatttcgac
    18481 tacagcattt ggacccccaa cataggcgta atcttctact tctcttaaaa catgcccaaa
    18541 ggccgtattc gtcaaatcat ttcttaattc ttcaggcgat tctgctaaag cgcttacaac
    18601 aagatacgtt ccttttggga atgggatgat tcgcgtagtg tttggaacat cctgactcgt
    18661 ttgaacaccg acatagtaca tcattttccc gttatagggt tcgttgactg taaagaaata
    18721 atgattttca gcagcttgtt tcaatgcatc tattgttcca ttaccgataa ctttttgctc
    18781 aaatgcttgt ttctctgcag ccaaaccgac aaaatccgta ttgctactct tcaattccac
    18841 accaataccc gtcaccgtaa aatctggttt ttcttcgatc ctatactttg ccatttctcc
    18901 caccctttct attatattaa gttcatctta cataaaatta aagggaaatg acacgattac
    18961 gctctttttt ctagggatat ttttattttt taactatttt tttgctttca ctgcaatcca
    19021 aatctcactg taaacattac tgaaatcctc tccccatttt gtaaaagaga tctcgggagc
    19081 ttcgactaac ccataatctg aagaaggaag ccattctgca tagattttcc cccaagtatc
    19141 ttgtaaagtt tggggaaatg gccctttatt aggaaaaatg gcccaagtgt gttcttctat
    19201 agcaagcatt tctaaatcag cgtacggatt ttcttttgtc gtagcaaagc caatcatgtg
    19261 catcaaactg cccttttctt ccaagcggtc tccatcaaat ccataagaaa cgttgattac
    19321 ttgatttgga tataaatctc ctaacttatg catctcttcc ctttgagtct ccgtgatcgt
    19381 ctgcgctaac ttttgtattt cttgattgac tccttcaaat ttgacaggaa cttttttcgc
    19441 cacacctgcc acataaaact gttctttctt ttcgatacga aattccatcg attttcctcc
    19501 tctgacatca ataaaaaatg tcaagcgtgg aaaagatttt tgtatgccgt atctactgat
    19561 ttctgaaggc agatatcctg accaatcacg aaaagctctt gaaaaacctt cgactgtttc
    19621 atatccatat ctaaacgccg tttctgtcac tcttgatcca tctagaagat cgcgattagc
    19681 caaagccaat ctacggtttt tgatatagtc cattagcgac atccctgcaa taaacgtaaa
    19741 gatgcgtttt aattgatatt cagagattcc tgtattttcg gctattattt tataggaaag
    19801 tccttctgtc aaatgttctt cgatgtaatc gatcaattgg ttcagatctt taagcatatt
    19861 tatcttcctc cttgattgcc tttttatcat attatcaaag aaagtcaatt acttgatgaa
    19921 tcaggaacca atttagtcag atgtagaaaa tgttgtatgg atctaaagaa aattctcaag
    19981 cagcgtttcg taacggataa ttggtattat ctgcctatct atgttttacc tttagaaaaa
    20041 taaattttgg ggaagacaga ttctaagtaa acaaagcggt aaaaatatct tttattccct
    20101 tagttaatcc gcttactacc tactgtacaa agcaaattgt tgttcaaact gctgatcaga
    20161 acgctgcttg gatttttctt tgcgatacat catacctctt ttatcaattg gaacaattat
    20221 ttatttctaa caaaatcact tcatttttgt cagataaata aaaaacaaaa aaagttccat
    20281 ctttaaaaca cgctattctt ttactttttc tcacattctc taagaaaaga aaaggagaat
    20341 gaatttttga caattcgagc aaaagtgatt atcaaatatg taaaatcagc tacttttttc
    20401 ttaaacgttt ctttatacta aatataaata agtgtttcat gaaaggagaa ctagatgaat
    20461 aattggcttg agtattttcc tgaaaacgtt ttggaacgcg gttacagcta tcatctgcac
    20521 ggttttgtcc gtcatttgaa ctatacaagc aagtacttgt ccgcaacagt ttctggcacg
    20581 gaagattata aggtagtgat cacttgggat gaaaagacca acatgacttg tgattgtcta
    20641 tatgccatag aaggaaaaaa atgcaaacac atggctgctg tcttattcgc ttacgaagaa
    20701 cgtccaataa aaaaatccaa ctactcactt tccgaactct cgtcccttgt ttcctccgct
    20761 agttcctctc ttgtacggga gcttttaaca gagattctta tagaacatcc gcactttatc
    20821 gaacgcttca aagttaagat gccatttcat gcaataaatt attcagataa attaacaact
    20881 atcatccata aatacgatca tattatcaag aaaaacaaaa acaggaaaac agcgaagttc
    20941 atcatggaga tgcgaaagtt catccaagaa gcagttgaat cgcttattca gcaaaatgcc
    21001 tatcttcccg cattcgaatt gatcaatgaa gtgattgcta ctctcgaaac attctattgg
    21061 gagcctgagg atgaacgaac ccttcttctg atagaggact gttattattt atggaaagaa
    21121 ttattagcag aagcacctca tgcagaaaaa aggcaaatgt tctcttggtt cgtctgccaa
    21181 gtcgatcata ctgacgcctc ttattcaaaa cgctactcta taaaaatcct gaaagaagac
    21241 tttcgggaaa aagaattttc taaccaaaag aaaaaaatag ataaaaaact aaagaaacgg
    21301 taaagaaaga cgatttcaat gaaaaatagc aagtaaacga taacaaccat cctaaatcca
    21361 caagaaatac agcagacctt gctttaaaaa cacgaatcat gcaaaggatg aagtaaagaa
    21421 gacttaacgc gtacccaata ttagtcattt gactattagg tacgcgtttt gttttataag
    21481 tcatgacaaa tttccttcgt tgctggatag attttccggc attgttgata gattttatga
    21541 tacttttcta cattttgtaa gagaggttcc gctgcatctg tatattggat aaaattctga
    21601 caacaacttt caggtgtgtc gaaccaccct aaccctagtg cagcaatcat tgttgcgcct
    21661 aatgcaggtc cttcctccac acttaaacaa tagaccgttg catcgaaaat atcagcttgg
    21721 atttgtagcc atgcaagatt tttcgcgcct cctcccaccg aaacaatacg tgtaaatctg
    21781 cgacccgcta gttctttacg tttcaatccc accattgaca aatgctcatt aaaaatttag
    21841 aatattcaca agtttaacga caattatgaa aatttatgtc gtgctaaagg agttttgtta
    21901 ttggatgaaa agttagatat aaaaactgcg atcaaagaca cgttacctac cgttttcggt
    21961 tatatcggta ttggacttgc atttggtatc gttgggaaag ctgccggatt tcatccacta
    22021 gtcgtcacgt tgatgtccct gctggtctat gctggttctg cccaatttat cacagtcagc
    22081 atgcttgcta gtcacagccc attgctttcc atcgttttct cgacctttct agtcaattcc
    22141 cgaatgattc tgatgagtat gacgattgct ccttatttca agaaaaatcg gctgcttcaa
    22201 aatctgctga ttggcacatt gctgacagat gaaagtttcg cactgggaat gaataaattg
    22261 aactatactg gacaaaagtt aaactttcgt tggatgaata cagctaattg gatttcttat
    22321 ttgacgtggg tgggttcttc tcttgttggc gcacttttag ggaattttat tactgatccg
    22381 aaaaagtttg gtttggattt cgccatcgtc gctatgttta tcgggttact ttatcttcaa
    22441 gtcatttctg atagaaacac ctctaaacga ttacagttga tcctgattgg tctcactttg
    22501 attctggtat atgtcggact gatttttatc ccaagcaact tagtgattgt agttgtaaca
    22561 ctgattgggt gcggattggg ggtgtggatc aaacatgcct tcttttgagt ttacattgct
    22621 gactatttta ggttgtactt tagcaacttg gctttctagg gtcctaccat ttgtcttact
    22681 gaaaaaattc gatttaccgc aaccgttgct tgagtattta agttttgtcc cgatcgtcat
    22741 tatgtctgct ttatggttca gcagtctatt tacccaaaat atcggacatc tgccgcagat
    22801 caatctcgaa aatgctttgg cttcagttcc tacactttta gctgctattc tttcaaaaag
    22861 cttattggtg atcgtcttgg caggaattct ttccctttcg ttgattcgct tattataaat
    22921 aaaaaaacaa gagcttatgg caaattactg tcatgcgctc ttgtttttat caaagtcaaa
    22981 taagaaaaaa atcggcgtat tcttgcttat ccgttgaacg gaccactaac aacgactttt
    23041 cctaacatat gtcctgtttc tacgtctttg gttgcttgtt tcaagttctt cgcattcatc
    23101 ccatctgaat agaccttgct caatgtactg cggataatcc cttcatttgc taaagcagca
    23161 atgtgcgcta aagcttctcc ttgcgtttcg atttcatagt tatagtctgt tttagcgaac
    23221 atatactccc agtcgaagct gatagattgg tttttccaaa cacctatatc taacggctct
    23281 ttgatcccta cgatcgttcc cacatgtcca aatggtcgga caaggtgctt gatttgatca
    23341 aagtagcggg taatatcgaa taatacagcg acatactcta cttgatcgat tcctaattca
    23401 cgaaaacttt tacttaagtc gtgatgataa tcgattggat agtctacacc tgtttttttc
    23461 agccattcaa aattcttcgg acttgcagtc gcgtagactt ctaggccagc ccaatgcgct
    23521 agttgattca aaatcgaacc gacgcccccg gatccgttga tcactaagat ttttttcccg
    23581 cgattggcat tttcttctgg agttagacca aatttttcaa ataacaattc ataagcggtc
    23641 aatgaggtca acggcaaggc tgcagcttct tcatctgaaa gattttttgg tgcaagagcg
    23701 acaatacgtt cgtcaactag ttggtattct tgattgcttc ctgctcttgt tgttgtacct
    23761 gcgtaaaaaa cgcgatcgcc gacagaaaat ttttgcactt cgtcacctac agcaacaact
    23821 ttcccgactc catcaaaacc taagatacgt aaagcatttc ttatcccgtt atcttggcga
    23881 agtttcgtat cgacaggatt aacagatact gcgttgactt ttaccagaag atcccgtgaa
    23941 ctgggtacag gtatctgttc tttttcatca ataaagcttt ttggatcatc gatcggcaac
    24001 ccttcataaa aaccgactac cttcattaat tccttttctg acatacgaat tcctccttag
    24061 cttcatcaag ttggagcatc catacagtgt tcatttcaat atatatagta tgctatttgt
    24121 tccacctttt ttattcatca taattattct cttacttaaa gtaaactatt tgcacttttt
    24181 tggttaaaaa agaggttgcg acaagacttt tgtcacaacc tcttataaac ggtgttctcc
    24241 taccaactct tcggtcacaa gtcaaaatat tcatcaaaca tgagaagctg ttttttgaaa
    24301 ttgctccttg tgactcagag ttaaacggca ggctcacaac ctctgatata aacggtgttc
    24361 aatcaactga ccctcggtat tagctcaaaa aacgacaaaa tatgaagagc tatttttgtt
    24421 tggctgttct tattacttga tacaggacag ctttttcacg acctctaatt cacggtgttc
    24481 aagcaagaaa tcattgttca ctgaaaagcg ttggatgttc tttttcaaag gtctctaaga
    24541 tctctcgtac ttcatcagta gatttcgtat tcatcaactg aactcgcaag tcatttgctc
    24601 ctggaaatcc tttgatatag attttaaaga aacgatgtag tcctgtgatg gaacgcggga
    24661 tgatttctga ataatgatct tgcagatcta gttgcagacg aagtagtccg atcagttctt
    24721 tttctgtatg ttccctcggg tctttttcaa atgcataagg attcttgaaa atccctcggc
    24781 caatcatgat gccatcaacg ccatatttct cggccaattc ttctcctgtc tggcgatcgg
    24841 ggatatctcc attgatcgtg attagtgttt gtggagcgat ttggtcacgt aaagcaatga
    24901 ttttaggaat gacttcccaa tgtgcatcta ctttactcat ctctttacgc gtacgcaaat
    24961 ggacagaaag attggctata tcttgatgaa gtaggtgact gatccattct tccatctcag
    25021 acatttccgt atagccgata cgcgtcttga cgctgacagg caaaccgcca gctttagctg
    25081 cttcaatcaa ttgtgccgct acttctggac gaaggatcaa gccactgcct tttccacgtt
    25141 ctgctacatt agggactggg cagcccatgt tgatatcgat tccttggaag cccatttctg
    25201 ccatagcaat gctcatttct ctaaaaaatt ctggcttgtc tccccaaata tgggcaacca
    25261 tcggctgttc atcttctgta aatgttaatc ttcctcgtac actgtctttt ccatctggat
    25321 gacagaaact gtctgagttt gtaaactcag taaagaagac atccggtgct gctgcatgct
    25381 ttacgacatg gcgaaataca acatctgtta catcttccat tggtgctaaa acaaaaaaag
    25441 gctttggcag ctctttccaa aaattagtag tcatttattt gttactctcc attctatcaa
    25501 gcagtgaagt cgcaatcttt ctcaaaaaca ttgtcattat actaggaaac aaaaaaagaa
    25561 gcaaagtgaa cagcttaaaa gatgtttctg attgagcaaa aaaaagactg tgacaaaaat
    25621 cttatcacag tccattttcc ggaataaatg gtgttcaaaa gcttgcattt gcggtaataa
    25681 gtccaaccaa acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg
    25741 cagaacagct ttttcacaac ctctccacta aacggtgttc aatcagctga ccctcggtat
    25801 tcgctcaaaa aacaacaaaa tatgaggaac tattttcccg tttgtttctc taatactcag
    25861 gtcaaaacgc tgatttcaca acctcttttc taacttctca atcctcttcc tttattgatc
    25921 aaataccaag taacggcgta caaaacaacg atgaataaca caagtacgat catcgatgtt
    25981 gctactggca catcgatcgt tccaaggaat ccgtaacgga aaccggagat catataaacg
    26041 atcggattga cttttgaaac agcttgccaa aagggcggta acatagagat ggcatagaaa
    26101 acgccaccta gataagttaa cggctgcaag acaaatgtag gcacgatcga tacatcatca
    26161 taagactgag caaaaatccc gttgagcagt cctgctaatg aaaacaagat tgccgtcatc
    26221 aataatgtaa tgatgaccat gaaccatgaa tacacatgaa gtggtacaaa gaacaaggaa
    26281 atgatcgtta cgagagagcc gacgagaata ctgcgaccca caccaccaat cacaaagccc
    26341 caaataatca catgcgtagg aacaggtgct accaagattt cttcgatatt tttctgaaat
    26401 ttttgagaga agaaggacga agaaacattg gcatacgaac tagtgatagc agacatcata
    26461 atcaaaccag gaacgatgaa ttccatatat gaaaaaccac ccatgtcacc gatccgccca
    26521 ccgatcattt ttccgaaaat aacaaaatac aaggatgtcg tgatcactgg cggaaccagt
    26581 gtttggaccc atatacgcaa atagcggttc gtttccttga tagctaaact ttttaatgca
    26641 gtaaaataaa gattaaacat ttttctcctc cgtatgatta ttttcttctg tgattttcaa
    26701 gaacagttct tctaaacgat tcgacttatt cctcatagaa agaactttga ttccttgtct
    26761 tgtaagctga tcgaagattc cgttcacccc ttgatttcgt tcaacttcga cagcgattgt
    26821 ctgctcatct tcaaatgtac aagcatatcc ttcaatcaca ggtttggtat catatggcgc
    26881 tagatcaaag ataaatgttt caaattgcag ttttgacaga agctctttca tacttgtatt
    26941 ctcgatcagt tcacccgatt ggatgattcc gatgttgcga caaagcattt cagcttcttc
    27001 tagataatgc gtcgtcaaaa tgatcgttgt accattttcg ttcaattctc gtagaaactc
    27061 ccacatctcg cgtctcaact cgatgtcaac tcctgctgtt ggttcatcca agatcagcaa
    27121 acgtggttca tgcatcaatg ccctagcaat catcagtctt cgcttcattc ccccggaaag
    27181 catacgtgca cggacatttc tcttttccca aagattggac tgcttcaaat atttttcgct
    27241 gcgtttcatc gcttctttgc gagagacacc gtagtagcct gcttgattca cgacgatttg
    27301 ctggaccgtt tcaaaagggt taaaattgaa ctcttgtgga accaatccaa tctgttgctt
    27361 tgctcgaact agatccttgt ccagatcata accgaagaca ttcacttttc cagatgtttt
    27421 attgactaaa gaagtaataa tcccgatcgt tgtagacttc ccagctccat ttggccctaa
    27481 tagcgcatag aaatctcctt ctttcaccga aagatcgatt ccccgcaatg cttcgacccc
    27541 acttccatag atctttttca aattatttat ttcaagcgca tttgccattt atttcctatc
    27601 tccttataga catgattctt aattgtatca tggaatacag cagatgaaaa aacaacttgg
    27661 cttccacaca aaaacacacc tgctaacaaa gaggagatgt gttttctagt tcatgatcat
    27721 cttcgataga ataactatct aaacaactct ggaactttta aaatcataaa agtaccgaca
    27781 gcaactacta cgatgacata tagaagatcc ttataactga ggttcttgtc ttttttattc
    27841 atgttaagct ttcctccgtc cttttatcga aagctttttt cttttatttt aaccgaaata
    27901 aataaaatgg aaatagttat tttcaatttc tgttttcgct gtttgtttta cggagaactt
    27961 tgccatggaa cgtgcgtctc gtcgctgatg ctaagttttt tcgataaaag ctgctagaag
    28021 gaagacagcg tggtttattt tgtatcgtct tttgctaaat cttccaagat catctggaca
    28081 gcagactcgt tcccattgaa ttctgccact tttttggcat caatgtactt cttttgataa
    28141 tcgtcttttt ggacccacgc atcatgcttc gtttgataaa gttaatcaaa cggataccac
    28201 tgacgctgtt catcgaacaa caaactttct ttttgggcct gttcaactat ggatttagga
    28261 taattcaaaa ctttcagcaa ggcaatcgca tttcgggtcc ttgaaggtcc ttcttttaat
    28321 ttgaaatcaa aactgattcc ttttccttct gttacttgtt cttcaaagtg gacattctca
    28381 caataattct ttaaaatctc tgtcaattcg atatcatgtg tggcgacaaa tgctaaagat
    28441 ggatactccg caagccaccg aacgactgaa gcggaagaag caatccgttc gatcgtattt
    28501 gttcctttta aaatctcatc tacgaagcag tagcagcgtt ctttggaagc cacttggtcc
    28561 agcaaacgtt tgatcgattt gatttctgaa acaaaataac tgtcgccttc aaataaatca
    28621 tcttcgattg ccatggaagt caacacatgt ccaggttgca gggtaaactg ttctgcaatc
    28681 gctgtttgga tcgtctgtgc tagaatacag ctgatggcta tacttttgac atacgtagat
    28741 tttcctgatg cattggaacc cgtgactaat gtattcttct gccagttgac tggattaact
    28801 accgcttctt ttaacaatgg atgatagata tctttcgctg tcacaccgcc ctcagtaaac
    28861 tctggtcggc aagtgatcgg catataggtg cgataattca aaacggctgc tgcaacttcc
    28921 aattctccca gcaattccca cagcttgatg gcttcagttg aatgcttttc taatttagtg
    28981 agaacgaaat gataggagat aaatggcagc atgaacatga tgttgacata ttcaaaaatc
    29041 aattctgctt cactcccgct cttcattcga aatgaaaaac ctaatttagg gatgtctctt
    29101 aatggtttag tttgctgttt cagctctttt tgaagtggtg tcgtcatttt tgctaactga
    29161 ttggcggctg aaatcgttga aaccaaataa ctcatgctat tcagctctgt ttctagtgtt
    29221 tgtttcttca ctagataata gaccgtattg aatactaagc caccgattgc tagatagatt
    29281 cctagtactt gccctaaaag aagcaaacct acacccacca gcggaagcaa gccaagaaaa
    29341 ataaataaac ctacgcgtcc aatcttttta ctttttccat ccgctagata ttgttttgca
    29401 aaattatgat ctttttttcc taaacgagcg aattgatatt ggattctttc tctcagttga
    29461 ggattttcct gataaaaagc aattagtttt tctaactgat aatcttctcc aaaatcaaag
    29521 ctacgtaaac gctgatacaa cgcttctgaa cctacactag aataggtact gttgatccct
    29581 tcaaatactt cgaacatatc caagtcatac caagtaagat cgtcgatttc actatcccat
    29641 gaacggaatt ttttctccgt gagccacgca tccttcaaac tttcttcttt atcaaatctc
    29701 ggttgataag gaatttttcc ccaattacta cgaactgttt cctttacttt caaacgtgaa
    29761 aaaatgtcta aaacaactaa taaaataaca actgcaataa taattaaaac gataaattct
    29821 ctttggccca tatccctctc cgttccttga tttgttagtt ttagtatagc actgttgcgc
    29881 tatttgaaaa gggggtacaa aaataactgt cttcgttacc taattcgtat caatcacacc
    29941 aaaataaaaa caccttccaa aaaagaagat gtctttaaaa cgagatacaa ttttttgtca
    30001 ttcacttcag gtaaattaga gccaattaaa ccctccgcaa cttgacggag atggagaaga
    30061 tgcccccgtc taatctagcg gtcatctctc ctcccattcc ttccaccaat ttttttgaca
    30121 ggtaaagccc gattccgcta gattccgtat ttgtgcggct ggtattttcg gtataaaaac
    30181 gctggaagac tttttcaaca gaggtttgcg ctcctggttt gataggattg gccactgtta
    30241 atcgaatgaa tgaatcctct tttgaaaatg tcacctttgc ttgtccatct gcataacgca
    30301 ataaattctg tatgatattt tgcagcactc gcagcaacat gagcggatct gctaatacag
    30361 ttgtatttat cgagtgttca gcgaacgcta cctcgatatt tttctcagaa aattgttcat
    30421 aaaaagagaa aaatacttct tccacctctt tagaaaaaga aagcgtatgg agatccattt
    30481 ctttttgctt cgtctctatt cgagcaagat cataaaactg atccgtcata ctgagtaatc
    30541 ttgcaacaga ttgtttgatg atcgttaaat acttctcccg tttctccgta tcctctacct
    30601 gttccagtaa ttgtacataa ccgttaatag cagtcaaagg agttctgaga tcatgagaaa
    30661 ggccgacgat cgatagtctg atgttttctt ccatttcttg aaatgcttgt cctttttgtt
    30721 cattttcatc cacccatgta ttcagtgcat ctactaggtc cattagattc ttttctcgaa
    30781 attcgactga aagacgacca ccatattctt gtcgttcaga aagagtggcg attttcttag
    30841 ttaatctctt gatttccctt tgcaggaaga aaataaaact tcccataaag ataacaacga
    30901 gaagcaacag ccacttaatc aaatctaaac cccactcccc acaccgtatg aatatacaca
    30961 gcatgtgcct ttgctagttt ccctcgaaga ttggaaatat gcatattcac tgttttctca
    31021 ctatcaaaat atggttcttg ccaaactgtt tcataaagat ttgctcgcga aaatactttt
    31081 ttcggatgtt ctaaaaaaag cttcagcaga tcgaattctc ttcctgtcaa atggacagcc
    31141 acaccattca catttacatc cctcgactct ttgtccagta agatttcctt gtaatagatg
    31201 cgttgcgaca gatcttttac ttggacagaa gtctgatgac gaagttggat gccgattcgt
    31261 aacaacagtt cttcgatatc aaatggtttc gtcacatagt catccgctcc tgctgccaac
    31321 aaggaaactt tatctctttg atcgcttttt gctgaaagaa cgatgatcgg aacattcgat
    31381 gtctgtcgga tttctgctaa caatgcttca cctgaaagcc ctggaagcat gagatctaga
    31441 aggatcaaat cgacaggata tgtgcttaac aataattttc cttccgtacc tgaataagca
    31501 tcaagaagtt tgtatctctg ttcaagaacc tcctccagta aagaatgaac agcttcatcg
    31561 tcttcgataa tcaaaatagt ttccagcagg aacacctact taatgtcaga tttttggaaa
    31621 ataaacattc ctagagctga tgataccacg attgttacta gactgaccag aatcattttc
    31681 ggtgcttgcg aataagttgc ttgttgcgca aagtccgtat acatgagcca cgtttccgta
    31741 aaccactccg ttagaaattc caagcctttg atttttgcga aaacaccggg aacgaaggcc
    31801 gttcccatga tataaagaaa agaaagaagc aaactgacac tggtacttct agtgatgaat
    31861 gtcaaagcca ctggaacaga taatgtcgct aatatcagca gaagcaatac aggaaatact
    31921 tttagaacat aatttccata gtttttgatt ccctcaactt caccaaatcc gaaagcaagt
    31981 gtgtaagcaa tcatggctag ccccgtaatc aaaaccacgc caagtgttgt gatcaaaagc
    32041 gtaacgagaa gtttcgacac ataataagtc attcgattcc gtcctaaaga taacgtattg
    32101 cgaatcgtac cgttttgaaa ttcttcccca atataaaagc acgcaaatgc tgcgaaagcc
    32161 agaagaatcc atgcagccat tttttccacc attaaaatcc ctgttgcacc gttgatgact
    32221 gtatccgttg cttggtttaa ttcattgccg tattttgtat gcatatacca ttcgttccaa
    32281 gaataaaaaa gcggaagtac gaccatcagt cctagtgaga cccacatttt acggctcttc
    32341 catactttta ttttttcagc agttaataga ttaaacatgt ggattgcctc cagttaattg
    32401 taagaaataa tcttccagtt tttgcccaac taggtggata ccatccacag gaacttttgc
    32461 ctctaccaat gcttgattga tggctgctac ttggtcaatt ccttcaaaaa gctggataac
    32521 acggctagat tggacaaaat aatccctata accaagagag tcgagaaccg ttactgcttg
    32581 ttccgtcgcc gaagtgtcaa tttttataaa ttgacgagat tcttgtgcca attcttcttt
    32641 cgataattct ttgatcaagt gtccttcatg caaaataccg tagtgagtgg cgatctgtga
    32701 caattcatct agcaagtggc tagaaagcaa gacggtgatg ccccgttctt ttaccaaacg
    32761 ttggatgatt tctcgcattt ctgccatccc tgctggatcc atcccattcg tcggttcatc
    32821 caaaatcaaa aattctggat tcgtgatcag tgcatttgca atagctaaac gctggcgcat
    32881 acctaaagag aaattctttg ctttcttttt ccctgtattt tccaatcgca tcattttcag
    32941 caggtcttcg atttctctat ccgacacacc gccgttcgct gcttggatac gtaggttatc
    33001 tcttgcagtt aattctggat aaagagcagg ggtttcaatc gattgaccca tacgtcgtct
    33061 tgctgcctgc aaatccgtcg ttccaaataa ttcgatcgaa ccttcatcaa tcgagatcaa
    33121 acccataata gttctcataa aggtagactt acccgctcca ttctccccaa tcaaaccata
    33181 gatcattcca cgtttgattt cgatcgatac tttatctaat gctttggcag caccgtattt
    33241 tttggttata ccagttgctt tcaagaccgt ttcatttcca aaatccatac ttatccttct
    33301 ttcttattga taagtgaagt atagttgacg ggtataaaga aagcagccag aaatgtataa
    33361 agaaagtgta aattttccaa ttgaatttca agaaaaatca gactgtctcg cagacgtggt
    33421 tgagttgtct gccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc
    33481 atggtaatcg ttgttatcgt tgacatcaga cgtccttgta tatgcgttgg aacttctcgc
    33541 tgaagccaga ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa
    33601 aaataataca tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag
    33661 acttttcaca atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa
    33721 gtcacaatat ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag
    33781 ctgaccactt ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat
    33841 ctttttcccg tttgtttata gatcgaatga tattatagac caagaatcct acctattaac
    33901 atagctaaaa acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga
    33961 ttaaggtaac tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg
    34021 aacgataatc gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct
    34081 ggttttttct tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg
    34141 taaatatatt ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta
    34201 atttaaaggc agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat
    34261 ccattctatt cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga
    34321 gcatcttatt tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa
    34381 aagaaattat gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt
    34441 gctcctacga ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta
    34501 gatttagcac atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa
    34561 gtcactgttc cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa
    34621 ccaggagtcc cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat
    34681 atgtccgata aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat
    34741 actcatattg tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca
    34801 ataaaacgat cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa
    34861 gccattgacg attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta
    34921 gggcaatcga ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt
    34981 gtggaaaatg ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca
    35041 aatgccaatt caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca
    35101 cctgtcgcct ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct
    35161 aatacctaca caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc
    35221 gataaagaat cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact
    35281 ttacctgcaa aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct
    35341 catggtacaa aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc
    35401 acccccatga ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca
    35461 cgggaaaaag ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg
    35521 aatgacaatt attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt
    35581 gaaatcgttc aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt
    35641 gctaatgcgt tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc
    35701 gtgaatacgt tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagccctt
    35761 gttaccagta acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa
    35821 gaagacaaag atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac
    35881 accttgttta ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag
    35941 ttagcgaagg gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac
    36001 caagttgccg ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc
    36061 gaaggatata gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc
    36121 gaaaaaaatg ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc
    36181 tttgatttct ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat
    36241 ctgaccttta aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa
    36301 gcgaccacag cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt
    36361 ccttatgggg attatttact tgaagaagta gaagccccag aaggttttca aaagattacg
    36421 ccattagaaa tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat
    36481 gtctttacca ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac
    36541 gagaagctga cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg
    36601 cctgaggaag aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta
    36661 acttctctgg attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa
    36721 gaagactggt atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa
    36781 aaagatgaaa aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa
    36841 gagaaaacag gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa
    36901 actattgtct tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag
    36961 ccagtagcga aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa
    37021 caccatgttt ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat
    37081 ggtgatgtgg tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca
    37141 aaagaagctt tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt
    37201 tggaaatctg gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt
    37261 gcgaaaaaag tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc
    37321 aattacgata aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct
    37381 caaaccttaa caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca
    37441 ccaactgttc caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc
    37501 ggggagaaaa attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg
    37561 ggctattatt tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag
    37621 aaccaacgtg cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta
    37681 acaatgaaaa gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac
    37741 ctttggaaag ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa
    37801 tactacggtg attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat
    37861 tgaggtggta gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa
    37921 agccgtgaat ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga
    37981 ttacgtgttg tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac
    38041 agaaggtatt gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg
    38101 ccgagaagta cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac
    38161 ctataattta aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt
    38221 acccttacgc gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac
    38281 cgttgccaat tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga
    38341 tttagtcttg aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa
    38401 ataggtgaag attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg
    38461 aaaaggcgag ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt
    38521 tctgattata catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt
    38581 actagactgg ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt
    38641 atgtcggcag gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga
    38701 aggcttttta aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac
    38761 tagcataact ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg
    38821 aaaccatcaa aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc
    38881 actttgttct tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc
    38941 aagagtttag acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt
    39001 ctataaaaga tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc
    39061 tttatattca cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc
    39121 ggatgattct agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca
    39181 aagatttgaa tagtagccga accagagaga ctatcagtta cttccccaaa atctattatc
    39241 gaatgaaaga tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc
    39301 agcttttaaa attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg
    39361 aactcaaaga ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg
    39421 ggatagacga agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg
    39481 cttatgattc tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt
    39541 tcctcctcac cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc
    39601 ccaaaaatgc agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg
    39661 aagaaatttc tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa
    39721 tgaaagaaat gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca
    39781 actttttaat ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg
    39841 cggtgatttt gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc
    39901 tgattttggc ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatca
//
LOCUS       DAFEPQ010000019        39074 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.019, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000019
VERSION     DAFEPQ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.019"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            6..305
                     /locus_tag="LP543_001095"
     CDS             6..305
                     /locus_tag="LP543_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2321272.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="LP543_001096"
     CDS             378..1739
                     /locus_tag="LP543_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBL2321273.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="LP543_001097"
     CDS             1761..2021
                     /locus_tag="LP543_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321274.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="LP543_001098"
     CDS             2035..2787
                     /locus_tag="LP543_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBL2321275.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="LP543_001099"
     CDS             complement(2881..3486)
                     /locus_tag="LP543_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL2321276.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="LP543_001100"
     CDS             complement(3539..4513)
                     /locus_tag="LP543_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBL2321277.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="LP543_001101"
     CDS             complement(4891..5610)
                     /locus_tag="LP543_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2321278.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LP543_001102"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LP543_001102"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBL2321279.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="LP543_001103"
     CDS             7161..8558
                     /locus_tag="LP543_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL2321280.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="LP543_001104"
     CDS             8639..9079
                     /locus_tag="LP543_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL2321281.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="LP543_001105"
     CDS             9227..9553
                     /locus_tag="LP543_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL2321282.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="LP543_001106"
     CDS             9554..10303
                     /locus_tag="LP543_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321283.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="LP543_001107"
     CDS             complement(10383..10838)
                     /locus_tag="LP543_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBL2321284.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="LP543_001108"
     CDS             complement(11265..11540)
                     /locus_tag="LP543_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321285.1"
                     /translation="MKLEDKKVILKKIKRLPEQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="LP543_001109"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="LP543_001109"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBL2321286.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="LP543_001110"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="LP543_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBL2321287.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="LP543_001111"
     CDS             14770..17436
                     /locus_tag="LP543_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL2321288.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="LP543_001112"
     CDS             17800..18552
                     /locus_tag="LP543_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL2321289.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="LP543_001113"
     CDS             complement(18574..19728)
                     /locus_tag="LP543_001113"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBL2321290.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="LP543_001114"
     CDS             19913..22354
                     /locus_tag="LP543_001114"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBL2321291.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(22684..23388)
                     /locus_tag="LP543_001115"
     CDS             complement(22684..23388)
                     /locus_tag="LP543_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321292.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(23575..23820)
                     /locus_tag="LP543_001116"
     CDS             complement(23575..23820)
                     /locus_tag="LP543_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321293.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(23837..24688)
                     /locus_tag="LP543_001117"
     CDS             complement(23837..24688)
                     /locus_tag="LP543_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL2321294.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            24854..25858
                     /locus_tag="LP543_001118"
     CDS             24854..25858
                     /locus_tag="LP543_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBL2321295.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(26099..26455)
                     /locus_tag="LP543_001119"
     CDS             complement(26099..26455)
                     /locus_tag="LP543_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL2321296.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            26823..28010
                     /gene="tuf"
                     /locus_tag="LP543_001120"
     CDS             26823..28010
                     /gene="tuf"
                     /locus_tag="LP543_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBL2321297.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(28198..30789)
                     /gene="mprF"
                     /locus_tag="LP543_001121"
     CDS             complement(28198..30789)
                     /gene="mprF"
                     /locus_tag="LP543_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBL2321298.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(30815..31576)
                     /locus_tag="LP543_001122"
     CDS             complement(30815..31576)
                     /locus_tag="LP543_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL2321299.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            31854..32375
                     /locus_tag="LP543_001123"
     CDS             31854..32375
                     /locus_tag="LP543_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HBL2321300.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            32434..33294
                     /locus_tag="LP543_001124"
     CDS             32434..33294
                     /locus_tag="LP543_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBL2321301.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            33479..35794
                     /locus_tag="LP543_001125"
     CDS             33479..35794
                     /locus_tag="LP543_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL2321302.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            35976..37817
                     /locus_tag="LP543_001126"
     CDS             35976..37817
                     /locus_tag="LP543_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBL2321303.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            37841..38950
                     /gene="rpoD"
                     /locus_tag="LP543_001127"
     CDS             37841..38950
                     /gene="rpoD"
                     /locus_tag="LP543_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBL2321304.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT        12571 a         6876 c         8095 g        11532 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttgctct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttgctt ttttctctat cagaaaatat
    22441 gtttattatt agaatttttt atagttaagg ttaggcatct taagaaatca ggcaactcca
    22501 agatatcttc aggtagaaga gaagtcgcct aacattgtag gatcataggt gatttgttgg
    22561 acaggtcaag ctgatatttg agcctgatgt tagaaggaat ttctttttac ttgctgtaga
    22621 aattattata tgactaaaaa ggctgggatt ttaaatccca gccttttatg acaaatagaa
    22681 tttttatcga tcagtaatcg ctgtcgctgc aaattgttcg atcaaaggaa tcaaagtcaa
    22741 tttgactttg tatttccgtt cgagtgcttt ttcgataacg aactcatttt cagtagtcat
    22801 agaaatagtt gctaatttac gaggttcatc agcatttttt tcattaataa ctacttcatg
    22861 ttcattttgt tctgtgtaaa atttaggata ctcagcaacg gcttttgaaa aatcagcaaa
    22921 acttaaagcg gcattcgtat cagttgagcg tgtattttca gttgtgtccg cttttggttt
    22981 ttccgttttt tcttctggca cactgactgt ttcttctgta actggtgtcg caacagcttg
    23041 cgcagttggt tcatcgtttt tcaatcgttg atcattccag taagacacga tttcaataag
    23101 aagattgtca cggaatcgtt cgaaaatccc gttttctcct gaacgaattc tctttaatac
    23161 agtattgaag ttgtcatcga ttggcacgtt gatccgtgta tcttcatttg ttttcccagc
    23221 taatccgatc gttaagattt gacggttttt gggatcaagc atgacttgta acggcaaacc
    23281 ataacgtcgc aagctctctt cttctagaga acggaataat tgtcctctta caccatccaa
    23341 tgaaggacgg aattgattta ataatttttg tttctgtttt ttcttcatat gttctctcct
    23401 tcgctcatac cctttcgatt ataagaaaaa aacagccaaa tgaaaaggag taatgcaaaa
    23461 aaacttgcgt atacgcaaat tacaacagaa aaatttgcgt attttcccgt tttttgaaag
    23521 aaaaataaaa aatggaacgg attatgtata tatacataat ccgttccatt ctttctattt
    23581 tttcggtatc aaggcaaaaa caggtagttc aggtgaataa atgacttcat gttcgtgcca
    23641 gggtttgacc attagcgaat gatcgaaccg ttttttcagt tcttcgatga tttgatcatg
    23701 tgtccagttt cttgcaggaa aatgttggat tttctctgca tcgatcaaaa agacaaaatc
    23761 ggaatgaaaa gtctgggtaa cttcttccga gagagaattc aagcgttcga ttggatccat
    23821 aaaatcacct ttcgacttaa aattgggtag tatctgggtc ttttgctttg ccgactacac
    23881 catctaactg atcgatttgt ttcatttctt cttctgttaa ttcaaaatca aataattcag
    23941 tattttcttt gattctgctt ggtgtcactg acttaggaag aggaaggaat tcgtgttgca
    24001 gcgaccaacg caaagcaact tgtgcaatcg tcttgtcatg tgcttcagcg atttgtttca
    24061 tttcaggcac atcgaagatt ttaccagtgc ctaatggact atatgcttcg aggatcatat
    24121 catgtttttt tgcatactca actacagttg tctgtaattc gcctggtgca aggaaaactt
    24181 gattgaccat cggcataatt tttgctgttt ttgctagtgt atctagatgg tggggtaaga
    24241 aattgctcac accgattgct ttgattttac ctgcttcata cagttcttca aatgctcgcc
    24301 atgtatctgc atttgcttgt tcccaattat cacgaaaagc aacagggttt ggccagtgga
    24361 tcaaaaacag atctaaataa tctgtttgga gcttcttcaa tgattcttca aaagaagaca
    24421 tcacaagttc atagctgtgg tttgcattcc ataattttgt tgtcaagaat atctcttctc
    24481 taggaattcc actttcctta atcgcttggc cgacgctttc ttcattcttg tatccttggg
    24541 cagtatcgat atggcgataa cctgcagcca gcgcttcttt aacagaggaa acagcgacat
    24601 ccccatcagg tgtttgccat gtgccgaatc ctactaccgg gatttcgtaa ccatttgata
    24661 gacgataagt gcttgttaat gaattcatac tttcagctcc ttttttattc gttcaactat
    24721 aagtgtagcg catttttcga aaaaaagaat ccattttgtt tctactcttg aaatttttgt
    24781 ttaaaagaaa atgattttga ttctggtatt tttgattttt tttccgtaaa ctaagacaga
    24841 agggaatgag aaaatggata tctatttaaa aaaagcaatt ctgcatatta ttgacagaga
    24901 aactggttct ccagtctttt cacaaaaaga gctggatctg acaaaagaat atattcgtga
    24961 ttttcttcaa aagaagatcc aaaaaatctc ttctgctcaa acaaaaactg gacaactagc
    25021 agaagaaagc atgatcgttg aatcacttaa aatcgctcaa gaaaattttg tggaagcaag
    25081 tgaagcaatc gttcagcggt ggttcgatat ctaccaagaa agtgaagaag cacctagtgc
    25141 agatgtattt gttgtcttgt atgaattaga tacagtgatg tacttatctt tattgaaagt
    25201 caattatcgt gaggcttaca ctcactttgt tgaagcagac gaagcaggga tcgacaaccg
    25261 cttgattttg aatcgagcaa ttctaggggc gaaaacacaa aaagcagatg aagcattcgc
    25321 tgtgaatttg actgattgga cctacgaatt aattgaaaaa aaatatgagt tttctggcaa
    25381 aaaagagctt tatttttctg gaagagtgat cgaaagtgtt cctgcacctt ctttagaaga
    25441 aaatgtaaaa gtgatccaaa aagtagccaa gcatcttgga aaaaaatttg agacagaaga
    25501 atttgatatc atggctgatt tgaaagaagc tgtctatgat acgatagaag aaaaaggtcg
    25561 gttggatcat gagatgattg cggaaaaagt cttcaaggaa aacatcacgg caaaaatggc
    25621 ttttaaagaa gaagtacaag agcaaggatt tgtcccagaa gcacctcctg taaaagaagt
    25681 acaagaaatt tctgagaaaa aatttggaaa acaaaaattc aagctatcaa acgggatcga
    25741 actgatcgta ccagtagatg tatatcgcaa cccagatttg attgaatttg tgaataatcc
    25801 ggatggtacg atatccgtga tgatcaaaaa tgtggatgaa gtattgaatc gtttataaaa
    25861 atgactagaa tattaggtgt tcaagtacca gtgatcagtt aaatgaattg tgtcaagaga
    25921 aggatcggaa aagacgaata taacaaaaaa agagcgtgag acaaaagtaa agaacacttt
    25981 tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt cttatttctt
    26041 ggagttaaac gcttctgtcc cagcctcttt ttatctaata aaattttaag accatttact
    26101 aattatcgat tttttctgcc gcatttttag gcacttcaga agcctttatt tcttcccagc
    26161 ttaatacacc tttacgagga ttgactttga gtttaagata cgcattcatc tttaatggat
    26221 gttctctttc ttcgcgaagc gtcacctgtt ttttttcacc atctttatta taagccgctt
    26281 gcttgtaggt ataagtcgtg aatttctctc cgttatctgc ttgggtagct gacatttcgc
    26341 cattcgtcgt gatttttgta taataagctt cgccgccgta aaaataatga taagctgcaa
    26401 aacttccgcc gagaaatatg cctaaaacga caagtacgcc aattaatttt ttcatgtact
    26461 tcacctcttt ccttattatt aacgaaatag aagatttcgt ctattgaaag ttgttggcaa
    26521 cagctgaatt ttaaactttt cctcattttg ttcttattgc ttcaaaagaa agttaaggac
    26581 tatcttgaag tttttctcaa aaaacacatt tttttattta taaaaggtac ttttttcgcg
    26641 tataagacag acgagtgctc ttagttgact ttgaccaata ttgacgtata ataagcttag
    26701 ttcgaatctt acaaaaagta agtcggacgt attctttttg atttagtcaa aaaaagacta
    26761 taataaaaag gtgagagctg aaatttttca gtgatctata ttatttagga ggtttgtaaa
    26821 ttatggcaaa agaacattat gacagaagta aaccacacgt taacattggt acgattggac
    26881 acgttgacca tgggaaaact actttaactg ctgcgatcac tacagtatta ggcaaaaaag
    26941 gcctagcaaa tcctcaagac tatgctagta ttgatgcagc tccagaagaa cgtgaacgtg
    27001 ggattacgat caataccgca cacgtagaat atgaaacaga aaaacgccac tacgcgcata
    27061 ttgatgcacc aggacacgcg gactatgtga aaaacatgat tacaggggca gcacaaatgg
    27121 atggtgcaat cttagttgtt tctgcaactg acggtccgat gcctcaaaca cgtgaacaca
    27181 ttttattgtc acgccaagtt ggtgtaaaat acctgattgt tttcttgaac aaagttgatt
    27241 tagtcgatga tgaagaattg atcgatttgg tagaaatgga agttcgcgag ttattgagcg
    27301 aatatggttt cccaggcgat gacactcctg tgatcaaagg ttccgcatta aaagcattgc
    27361 aaggcgatcc agatgctgaa gctgctatta tggaattgat ggatacagta gatgaatata
    27421 tcccaacacc agaacgtgat acagataaac cattactatt gccagtggaa gacgtcttct
    27481 caattacagg tcgaggaact gttgcctcag gtcgtattga tcgtggtgct gttcgtgtcg
    27541 gtgatgaggt agagatcgta gggatcaaac ctgaaacaca aaaagcagtt gtaacaggtg
    27601 tagaaatgtt ccgtaaaacg ttagattacg gggaagctgg ggataacgta ggcgtgttgt
    27661 tacgggggat ccaacgtgac gatatcgaac gtggacaagt acttgctaaa ccaggttcca
    27721 ttactccaca tacaaaattc aaagcagaag tgtacgtgtt gacaaaagaa gaaggtggac
    27781 gtcatactcc attcttcaac aactatcgtc cacagttcta cttccgcaca actgatgtta
    27841 caggaacaat cacattgcca gaagatacag aaatggtcat gcctggtgac aacgtgacga
    27901 ttgatgttga cttgatccat ccaatcgctg ttgaaaacgg aacgacattc tcaattcgtg
    27961 aaggcggacg gacagtaggt tcaggtatcg ttacagagat cgaagcataa gaaacgcgac
    28021 tcgaatattt tggtaatgaa aaatgtttaa aataaaagag cggtccaaac aggatttctg
    28081 tttggaccgc tctttgttct tcaattgtat aagaagagat tgtagaagca tgaactttcg
    28141 ttttacttca gtctctttta ttcatcatga gagtgttcat ctatcggtat gcagttttta
    28201 atactttctt cgtatccatt ttttcaaacc acttattcta ccttgtgtct gctcaactgg
    28261 cgcattatca ataatcagca aagcaatcat aacatatgca atccaactat ctctagaata
    28321 tagagtataa cgtgagaccc agtttgtagc gtacttgctt ttatattctc ttaatccatg
    28381 gaaagaataa aaatgagaac caaactcgta aaccaagtat gcaatccttt cctgtaaaaa
    28441 actctttctg gaagttccga catttgaaag aggggccatc cctaaattga accattggat
    28501 atgttcttct ttcatatagg caaacaaatg caagaacaag aaatccatac tgccagaagg
    28561 tgctttttta gaatcatatc gcatcagatc aatcgttcct actttattgt tcgtgtaagt
    28621 aggcatgatc gttgcaaaag cgacaatttc ttcttgggta tttttgacaa ctgcaatagg
    28681 cgctctttgt agataatctt cagagaaaaa gcctaaagaa aacccttttt cctttcgtga
    28741 acccaaccaa ttatcagaga tatttttgaa agtactcatt tgctcagtag aaaatggtgg
    28801 ttgtaagaca tcaaatgtaa atccttctcg ttcgatccga ttcaagacag cacgagtgcc
    28861 cttcattttt ttccctgaag ttgtaaaact attcaggtct acataagctt cttcccccat
    28921 cttaatgaaa tcgtagccga attcatgcaa aatcataaca atctcttcgc ttgtttcgta
    28981 gaaaactggt aaatagcata gtcgatctgt ttcttcgata aaagcttcga ttgcttctgg
    29041 aaaatcttct ttcttaccag aaggatcacc cattacaata catttattat tgaagcaagc
    29101 gaattggaga agaacagttg gttcaccgtc tttttcatag gcaaacatcc gcttatcttt
    29161 taagaagatc aattgactat cggagtttcc tccataagtt gtaaggatct tcagggcctt
    29221 ttcttcatta aaggcttcgc caatctgttt cttttctcct tgaaggaaat gaacaaatag
    29281 aacgatcatg aaactaacag cgatgatggc caacaatccc gaaaaccaaa tcttttcaga
    29341 aggaaataag aaaaaggaaa taaaatggtg acgccgatga gggaaatccg gcagattgta
    29401 taccccaata acgatgtaaa gcaaggacag tgcaccaata atgattccat cgatcgtacg
    29461 ccactcccat gaatagatca attgttcccg aaataattcg tttttagaag caagaatgat
    29521 cagaagcaga atagacaaga aaaccaccgg tgtgaaacta aaatcactta ataaaacata
    29581 aagtaaagct agaacaatca agaagattgt aggcaaatat gctcgtttca cacgtgctgc
    29641 gatccctctg cccataatga ttaaaagaaa tcccaatagt atactaggaa attggataat
    29701 aaaatgaaat ttcaatgggt tcaaactatg aagccagcga aactctgtaa aagcttgcgg
    29761 gatagtggca gaaagtacta gcatgattcc agaaaagtat aataagacaa ccacgatttt
    29821 atgcgcaatt tctgttgcta attgtttagg gataccagaa taacgctgat caaaagtgga
    29881 accgatattt ttgaagaaaa agacgattcc aatcaaaaac ggaatgatgt agtagaacag
    29941 gcgatagagc aagatccaca caacgactat ttctcttggc acaccaatag cggacaaccc
    30001 gataatcatc attacatcga aactcccaat ttctcctgga atcatagaca cgattcctat
    30061 cactgaagca gcaatgaata atggaattgt ttgccacaga tcaatccgga tttccattaa
    30121 ataaccaaca gaaaggaaac taaacaggac accggtccat tccaacaatg aaactagcaa
    30181 tagctgtcct tgagttttaa gtgaggtacc accaaggaat cctttctttt tgaaagtagt
    30241 aaataaaaag acgacaggaa aatatagacc gccgccgatc aaccatatcc aatattgctt
    30301 caaaaaagga gcaactggcg tgaagactac aagtaaaaaa gaaagcagac tgtaaataga
    30361 aagtccagcc atcaaaaaca agaaaacttt cgataatgct tggatcacgt ttctgctctc
    30421 tttcttttgt ccatatagtt cagagcgaag ccccacacta acaaacccgc cgaaaccggc
    30481 aatattgttg atcgtattga tcagccagct tgtctcaaac aggtaacgag gattctgctt
    30541 ttgttcaagc atccgattca aaataatatc ataaccaacc ataggtaaca cagagataag
    30601 accaatcaat agcattagtc cgcttttcca tacaggtata gtggcaaacg tttctcctaa
    30661 ttgctggata gatagagttt taccgatgga catcaattca ccgacaataa tgacaatgac
    30721 agaaatcaaa aagattgttt tgaacagtcc taggtggttt ttcagccatt gtatggtatt
    30781 ttttaacaaa taaatcacct tctattccta atagttatgt ttcagaaaga aattgaagaa
    30841 cttctcgatt gaagcgttca ggatcttttc tagctaattc atgtccttgt tctttcacaa
    30901 gcacaaagga agcttttggt atagttttag ctatatacaa agaatgtttt agcttgatta
    30961 catctttttt tcccacgatg atcaaagtgg gggagttgat tttttttaaa tcattttctg
    31021 taagcccgat gtcatgcagc aaaagtttga taactaaaag atttttgcga agacttgggc
    31081 gaaataaaga gagtaaccat acccatgcat aatgaatatt actgatgact ctggcagaaa
    31141 aacgaacgcc tttaacaagc gtgtttccag aatttaaaat caatcgatga acttttttag
    31201 ggaagctgct ggcaaatacc agtgctagat ttgctccatc actaaatcca agaaaatctg
    31261 cctgatcgat tttttccagc aacatgatcg tattcagatc ctcggccatt aactgaaaat
    31321 tcaacataga tgcttcgttt gtcgaacgac catgtcctcg gctatctact agatacacgg
    31381 tatagtagcg ttcgagtaca gggacctgtt ctgaaaaaaa gcggccgctg ccgtcattgc
    31441 catgcagtaa aaagagaggg aagccttgac cgctcttttc atagtaaatt tcgctcccat
    31501 ctggcatttt tgcatatttc ttttcttttt tcacataatc acctactgtg taaagtataa
    31561 cgattatatc agtcattggg caattgtttt tactgaaaaa caacgatcat tttcgtaaga
    31621 tgtcaaaaaa tacaggaaaa ttcatttttt cttaaggcgt acacagaaat caaggaagaa
    31681 atagccaaga agataaagaa atagtaagat aaaaaaagat gtcaaaataa ctaggtgatg
    31741 aaatgaagcg aaaacgatta tctattatcc gtattctttt ttacatgatc attctgacgg
    31801 ttattggtgc ggtgttgttt ttatgaggaa gaacttatcg ttcggtaaaa cgagtggaaa
    31861 gctatcaaat agaaataaca acagctgcaa aaaattatca tatggaaaac tacgagaatt
    31921 tgatcaaggc gataatattg accgaatcaa aaggaaaagg gacagatctc atgcagagct
    31981 cggaaagtct gcatggagaa gctgagatca ttgataatcc agctgacagt attgatcaag
    32041 gtgtcaaata tttggcggaa atgatagtag aagcaaagaa acaaggatgc gatttatgga
    32101 cagctgtcca agcatataat tttgggaaag attatattgc ctatgtaaaa aatcatggcg
    32161 gagaaaacac agtagaatta gcagaagcgt attctaaaga tgtgctgagc ccacttttag
    32221 gaaatgagga agggaaacaa taccgatatt ggcggatcca gtccttgctt tacaatggcg
    32281 gctatctcta tcataatgga ggtaacatgt tttatcctga tattgtcaaa atgaatgaaa
    32341 aaataataga gacctatgaa aaagtcttta agtaaaatag caggaaatgt tcaatcatga
    32401 ataaaattaa atgaaagaag gagtgcgcaa atagtggaaa aaaggcaaaa aatggaaaat
    32461 caagaagtgg ggtacacaac caaagactgg atgttacggt ttgtgaaggg aatgtttatc
    32521 ggttcaggat ttatcttgcc aggagtcagc ggtggtgcat tagcagctgt ctttggcatg
    32581 tatgagcggc tgatttcttt tttagcccac cttacaaaaa atttcaaaga aaatgttttg
    32641 ttttttctgc ctgttggtct aggcggagtg acaggcatct ttctactttc ttttttagtc
    32701 agttttttat taggtgcgta tgaaacaacg atattatggt tttttgtcgg atgtatcgct
    32761 ggaactgttc ctgcgttatg gaaagaagca gggaaaaaag gaagagatac ttctgatgtg
    32821 atcatcatgg ttgttacttt tgttctggct tatttgtttt tattatacgg cgctcaatta
    32881 tttgacgggc aagtcgatca gaattttgga acatggctga ttgccggcgg attgattggt
    32941 ttgggtatga tcgtcccagg attgagccct tctaattttt tggtgtatat gggattgtat
    33001 aaagcgatgg ctgacggatt taaagaagta cgacttgaag tgattgtgcc gatcggaatt
    33061 ggtgggattc tttgtgttct tggattgtca aaaatcatgg attacatttt ctcaaaggca
    33121 tatagcaagc tattccattt cattttaggt gtagtttttg cttcaacgat tatgatcatt
    33181 cctaccgatt acgcgggatt gggagttgct ggggtactag cttgtcctgt tctttttata
    33241 gccggtacag cactaggttg gtggatgagc cgtttggagg aacaatataa gtaaagatag
    33301 attgataata aaaacagcac acgggtgatt caaacaaaaa ggttggcatc ttttttgtaa
    33361 aatcattcgt gtgtttttct ataaatttta taaaaacaag ttgtttttaa tcttggaaca
    33421 cgctagactt aaacacgtga ttatttttga aagttggcgg aaaatggaga aaaagaaaat
    33481 gaatggactt acacagcaag aggtagttca gaaaatggct tctggccagc aaaatgacta
    33541 ccaggaagat gcatcaaaaa gtacaaaaca aatattcagc gataatatat tgacgttatt
    33601 caattttctg aactttggta ttgggatttg tctgttattt gtaggtgcgt attccaacat
    33661 ggcgtattta gcaattattg tcgtcaatat cgttattgga atttatcaag aaatacatgc
    33721 aagaaatctt gtacggaaat tatcgattgt ttcccaatca gaagtactag tgatccggga
    33781 cggtaaagaa caatcgattt ctactaaaga gctggtatta ggagatttgg taaaaatcgg
    33841 agcaggagaa cagataccag ccgatttaca ggttatatct ggacgggcag aagcaaatga
    33901 agctcttttg acaggcgaat ctgatttgat tgtcaaagaa actggagctg aattgctttc
    33961 tggaagcttt ttgacaagcg gttctgtcat ggctgaagtt atccgagtag gtgcagataa
    34021 ttatgctgtc cgcttgacta gcgaggcaaa gactcacaaa gctattcgtt ctgaacttgt
    34081 agattcgatt cgcaaagtat ccaaattcac tagtttcgtg attattcctt tggggatcat
    34141 actatttttg gaagcactgt tcatccgcga tgcttctatc aaaatttctg ttattgcatc
    34201 tgcagcagct ttgttaggca tgcttccaaa gggtttggta ttgctaataa gtattgcatt
    34261 aacgacaggg gtaaccaaac tggcaaaaaa acgaatcctt gttcaagaca tgtattcaat
    34321 tgaaacgctg gcccatgtcg atacattatg cttggataaa acagggacca ttactgaagg
    34381 gaaaatgagt atccagcggg tagaagcact acatccgctg tacgagaaag ccatcccaga
    34441 aattatgggg acgtatctgg cagaaagcag tgataacaac atcactatgc gggctatgag
    34501 agattatttt gagctttcta atcggtatga tgtgacaaac aaagtcgcat tttcttctga
    34561 tcgcaaatgg ggcgcgatgg aatttccaga actgggagtc gtgtacgtag gagcgcctga
    34621 acgtttggtg gaagaagatc ggcttccgga cagtctaaaa acagcccaag cgaatggcta
    34681 tcgcgtactt atgttgggga tcgctccaaa tcagcaattg aacgaagaaa aaccagcatt
    34741 tgttgagccg gttgctctgt ttgaaatcga tgatccaatc cgaaaaaacg ctcaagaaac
    34801 actagcttat cttcatgagg aaggtgtgga tctgaaagtg atttctggag acaatccttt
    34861 tactgtctcc aatatcgcta gacgtgcagg attaccgaat tatgatgcgt atgtcgatct
    34921 ttctcaaatt actgaagaaa tggaagtgcg agaagctgct catcgctata cggtttttgg
    34981 acgagtgagt ccgcagcaaa agaaattgct ggtaaatgaa ttgaaagaaa gcggcagaac
    35041 ggttgcaatg acaggcgacg gtgtgaatga tgttttggcg ttaagagaag cagattgcag
    35101 cattgctatg gcagaaggtg atggagcaac acgtcagatt gcgaatctgg tattgttgga
    35161 ctctgatttt actacattgc cagaagtcct gtttgaagga cgccgagtgg tcaacaatgt
    35221 gacgaaagtc tcaggcatct tttttatcaa aacgatttat tcatttattt tatcgattat
    35281 atgtgccgtt acagcaattg gatttccgtt tatccctatc caaatcacgc tgattgattt
    35341 agcgattgaa gggtatcctt ccttcttttt atcttttgaa caagataaaa gaaaaatcac
    35401 ttaccgttat ttgcctacag cacttattaa tgctttacca aatgccttgc ttgtagtagt
    35461 gaatatcatt gcggtgtatc tacttggaaa aacattcgca tggggaaatg ctgaaactac
    35521 tactttgatg tattacttgc tgattgggat cagttgcatg gcagttgtaa aagcttgtta
    35581 tcccttcaac ccattgcgca tcttcctagc agtaacgacg atcgtaggga tatatgtagc
    35641 tgcaatgctg ttccatggaa ttttggaagt aaacttaggg ttgggagaga gcgtaggata
    35701 cttctttgta ttcatgctcg taaatattgt tcttcgagta ttgtactgga aggctgatat
    35761 tcaaaatcag ttactgaaaa gagtagcaaa ttgatgctta atgagataga aatgtgttat
    35821 acttgaaaaa gcaatgaaat atgatacaat caaacatgta taattgtgtc ctttaaaatc
    35881 gcactgattt cagttagtgc gattttttac cggttattct cctgaaatat cttctgttaa
    35941 acccaaaaga tatcaataga gcagggggtg aaaaaatggc tcaacggatt cctcaagagg
    36001 ttatcgaaac agtacgcagt caaacgaata tcgttgaggt gattggacaa tatgtccagt
    36061 tgaaaaagtc tggtaaaaat tacttggggc tgtgtccttt tcatgaagaa cgtacaccgt
    36121 ctttttctgt tgcagaagac aagcagattt ttcattgctt tggttgtggt aaaggcggaa
    36181 acgtgtttac ttttcttcaa gaactagaag gtctaagttt tccagaagca gtggtaaaag
    36241 tggcagactt cgaacagata ccgttagaag atcaatggcg tcagcgtact atagctgtcc
    36301 aaaatcctga atctgctacg gggaaactga ttgctgctca tgaaaaagca gcggaaatct
    36361 atcatcacat gctattgcat acaaaagctg gacagccagc tttagaatat ttgcaaagtc
    36421 gtgggttgtc tttagaatta attgaagaat tcaacatagg ttttgcacct aatgagcgtt
    36481 cttttcttca gcaagtattc aagaatgaat caatttcaaa tgaattgtta gaacgctctg
    36541 gattgtttgt cacgcatgat gatggccgta tgtctgatcg tttttatcaa agagtgatgt
    36601 ttccacttcg taattctcaa ggccagacga tcggtttttc aggacgctgg cttgcacctg
    36661 aaaaagggca ggaaaaagat cagcccaaat acttgaatag tccagaaaca gaactgttca
    36721 ataaaagaca agttttattt aatcttgata aagcaagaag cgatatccgg aaaaacggag
    36781 aaattttgtt gttcgaaggg tttatggatg tgattgctgc atggcaggct ggtatcaaaa
    36841 ctggattagc ttctatgggg acaagtctaa cagctcaaca gatccaacaa atggaaaagc
    36901 tgacgaaaga attggtcttt tgctatgacg gagacaacgc aggttttgaa gcaaccaatc
    36961 gagggattga attgcttcgg caaaacagtc gattagcact ttctgtcgta gtcgttccag
    37021 aaaagcttga tcctgatgaa tatctccgta agtatggaga agcatctttc caagaattag
    37081 tggctcatgg gcgcgaaaca gtctttactt ttaaatcacg ttatttaaaa caagggaaaa
    37141 atttagataa tgaaaaagac aagatcgaat acctcgaaga attactagtt gaacttagcc
    37201 gagttgtttc accaatcgaa caagatatgt atctatctca actgtcaaca gagtttcaga
    37261 tcggcagaga gacgatacaa aaacaactgc gcgatcttcg aaggacaaat cagcaaaatc
    37321 gtcaagaacc tcaggaaagt caacacactc aggttacgag acaagaaact gcacggaaaa
    37381 agagaccttt gacgcaagta gaaaaggcag agcagattct attatatcgt gcatttaaag
    37441 aagtttctgt ccgtaatatt ttaaaagaac gagaatttca gtttattcat gatgcatatg
    37501 cagaagttta ttttttattc gatgcatatg tctcacaaca ttatgaattc aaccttgcag
    37561 agtttttaga ctttttgcaa gatgaaaatt taaggcggtt ggtggtggaa atcgaatatt
    37621 taccggtggc agaagaaagt tcgccacgag aaatccaaga cttgctagat gtgatcagca
    37681 aatctggctt agctgatgaa ataacattaa aaaaacaaat gcagcaagaa gccagacgaa
    37741 ctggaaacaa acagcttgag cttgaattga cgattgaaat catcaattta acgaaacagt
    37801 taaaacaagc caaatagcta ctcaaggggg gccttctttt atggcaaatg gaacagataa
    37861 aaaaacgtat gaagcagcag tagcagcttt catcaaagaa aacaaaccca aacgacaagt
    37921 tgtatatgat gatttatcca atcagcttgc tacaccattc actttgaatg cagatgaaat
    37981 ggacaaattg atccaaaaga ttgaagacgc aggtatcagt gtggtggatg aaaatggtga
    38041 tccatcaatt cacagcttga agagtgctga gaaaaaagct gaacaagcaa aaacagaaga
    38101 tctttctgcg ccaaccggtg taaaaatcaa tgacccggtt cgtatgtatc tgaaagaaat
    38161 tggccgtgtc tcccttttaa ctgcggaaga agaagtagcg ttagcattga aaatcgaaga
    38221 aggcgaccaa gaagcgaaac agcgtctggc agaagcaaac cttcgtttag tagtaagtat
    38281 cgctaaacgt tatgtaggac gaggcatgca gttccttgac ttgatccaag aaggaaacat
    38341 gggactgatg aaggcagttg aaaaatttga ctaccgcaaa ggatttaagt tttcaaccta
    38401 tgctacatgg tggattcgtc aagccatcac tcgagcaatc gctgaccaag cacgtacgat
    38461 ccgtattcca gttcatatgg ttgaaacaat taataaattg atacgtatcc aacgtcaatt
    38521 gctgcaagac cttggaagag aacctacgcc agaagaaatc ggtgcggaaa tggatcttcc
    38581 aacagaaaaa gtccgagaaa tcttgaaaat cgcgcaagaa cctgtttccc ttgaaacgcc
    38641 aatcggtgaa gaagatgact ctcacttagg tgatttcata gaagaccagg aagcaacaag
    38701 tccagcagaa cacgcagctt atgagttact aaaagaacaa ttggaagatg tactagatac
    38761 attgactgat cgggaagaga atgtccttcg cttacgtttt ggtttagatg atggccggac
    38821 gagaacattg gaagaagtag ggaaagtatt tggtgttact cgggaacgta ttcgtcagat
    38881 cgaggctaaa gcattaagaa aattgcgcca tccttctcgt tcaaaacaat taaaagattt
    38941 cttagaataa gcgtggacta ttgaccagaa gagaaatcag taaaactgtt tggtttattt
    39001 taaaagaaaa aaagcttgag actacgcagt ctcaagcttt tttttctata ggaaggaaat
    39061 tcattcatta tttt
//
LOCUS       DAFEPQ010000020        37245 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.020, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000020
VERSION     DAFEPQ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.020"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..839
                     /locus_tag="LP543_001128"
     CDS             <1..839
                     /locus_tag="LP543_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL2321305.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
     gene            complement(1263..1433)
                     /locus_tag="LP543_001129"
     CDS             complement(1263..1433)
                     /locus_tag="LP543_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL2321306.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1627..1806)
                     /locus_tag="LP543_001130"
     CDS             complement(1627..1806)
                     /locus_tag="LP543_001130"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321307.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1816..2040)
                     /locus_tag="LP543_001131"
     CDS             complement(1816..2040)
                     /locus_tag="LP543_001131"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321308.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            2193..3305
                     /locus_tag="LP543_001132"
     CDS             2193..3305
                     /locus_tag="LP543_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL2321309.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3383..3676)
                     /locus_tag="LP543_001133"
     CDS             complement(3383..3676)
                     /locus_tag="LP543_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBL2321310.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3844..4158
                     /locus_tag="LP543_001134"
     CDS             3844..4158
                     /locus_tag="LP543_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBL2321311.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4179..6158
                     /locus_tag="LP543_001135"
     CDS             4179..6158
                     /locus_tag="LP543_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL2321312.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6171..6497
                     /locus_tag="LP543_001136"
     CDS             6171..6497
                     /locus_tag="LP543_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBL2321313.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6513..7031
                     /locus_tag="LP543_001137"
     CDS             6513..7031
                     /locus_tag="LP543_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321314.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7031..8383
                     /gene="celB"
                     /locus_tag="LP543_001138"
     CDS             7031..8383
                     /gene="celB"
                     /locus_tag="LP543_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBL2321315.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8467..9972)
                     /locus_tag="LP543_001139"
     CDS             complement(8467..9972)
                     /locus_tag="LP543_001139"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBL2321316.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10064..10654)
                     /locus_tag="LP543_001140"
     CDS             complement(10064..10654)
                     /locus_tag="LP543_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL2321317.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10833..12479
                     /locus_tag="LP543_001141"
     CDS             10833..12479
                     /locus_tag="LP543_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL2321318.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12528..13247
                     /locus_tag="LP543_001142"
     CDS             12528..13247
                     /locus_tag="LP543_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBL2321319.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13726..14673
                     /locus_tag="LP543_001143"
     CDS             13726..14673
                     /locus_tag="LP543_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2321320.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14685..15605
                     /locus_tag="LP543_001144"
     CDS             14685..15605
                     /locus_tag="LP543_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2321321.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15646..17103
                     /locus_tag="LP543_001145"
     CDS             15646..17103
                     /locus_tag="LP543_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL2321322.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17448..18062
                     /locus_tag="LP543_001146"
     CDS             17448..18062
                     /locus_tag="LP543_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBL2321323.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYSSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18055..19779
                     /locus_tag="LP543_001147"
     CDS             18055..19779
                     /locus_tag="LP543_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL2321324.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19776..21227
                     /locus_tag="LP543_001148"
     CDS             19776..21227
                     /locus_tag="LP543_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2321325.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21700..23853)
                     /locus_tag="LP543_001149"
     CDS             complement(21700..23853)
                     /locus_tag="LP543_001149"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HBL2321326.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23855..24901)
                     /locus_tag="LP543_001150"
     CDS             complement(23855..24901)
                     /locus_tag="LP543_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2321327.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25051..26340
                     /locus_tag="LP543_001151"
     CDS             25051..26340
                     /locus_tag="LP543_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBL2321328.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26353..29055
                     /locus_tag="LP543_001152"
     CDS             26353..29055
                     /locus_tag="LP543_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBL2321329.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29040..29897
                     /locus_tag="LP543_001153"
     CDS             29040..29897
                     /locus_tag="LP543_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL2321330.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29968..30867)
                     /locus_tag="LP543_001154"
     CDS             complement(29968..30867)
                     /locus_tag="LP543_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL2321331.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            31036..33207
                     /locus_tag="LP543_001155"
     CDS             31036..33207
                     /locus_tag="LP543_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBL2321332.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33275..33616
                     /locus_tag="LP543_001156"
     CDS             33275..33616
                     /locus_tag="LP543_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL2321333.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33892..35646)
                     /locus_tag="LP543_001157"
     CDS             complement(33892..35646)
                     /locus_tag="LP543_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2321334.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35657..36493)
                     /locus_tag="LP543_001158"
     CDS             complement(35657..36493)
                     /locus_tag="LP543_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBL2321335.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36495..37178)
                     /locus_tag="LP543_001159"
     CDS             complement(36495..37178)
                     /locus_tag="LP543_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBL2321336.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12016 a         6552 c         7638 g        11039 t
ORIGIN      
        1 catatcagct ggtagaaact caagcgccaa cggggtatga attagatgcg acaccgatcg
       61 agtttgagat cgaaagaagt cagacagcgg tggtagaatt gacgaaagca aaccgactta
      121 caccaggcgg tgtcgtgttg acaaagatcg atgaccaaag cggggaagtc ctgcaaggag
      181 ctgtttttga gttacaagat gccaatggaa ccgttctgca gtcaggtcta accacaggag
      241 cagatggaaa actggcgatt gacggcttag caccaggagt gtatcagctg gtagaaaccc
      301 aagcgccaac ggggtatgaa ttggatgcga caccaatcga gtttgagatc gaaagaagtc
      361 agacagcggt ggtagaattg acgaaagaaa atcgacttac accaggcggt gtcgtgttga
      421 caaagatcga tgaccaaagc ggagaagtcc tgcaaggagc tgtttttgag ttacaagatg
      481 ccaatggaac cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgatag
      541 atggcttagc accaggagca tatcagctgg tagaaactca agcgccaacg gggtatgaat
      601 tggatgcgac accagtaact ttcaagatcg aaaaagaaca agaagtggcg atctttttaa
      661 caaaagagaa tagaaaagtt gctgatagct cagatataag aggaaacaat ccaacaagca
      721 gtggaaataa gcatcttcca aaaactggag aaaccatcat tagccagttt atattgtcaa
      781 tattgggtgt gctacttgtt ttcatcagta taaaatttag aaagaaacga aatatatgag
      841 ctaaaataaa aaattgaata agcatagttt ttaaaatcaa ctagtaccca tcaattgaat
      901 ggaaactacc gattttagaa ggggtataca aacaaatatt agtatcggag tagagaaggt
      961 tgaacccaac cagtaaataa gtaaagacaa ttttgtgctg cgttgagaag tgaaaaataa
     1021 acctttgtag taatactagt taataaaaaa tatacaaaaa cactgcgaaa atcattcttg
     1081 agtttcgcag tgttttttgc ctaatgactc aagtttattg ggtaatactt tgattttctt
     1141 tctacaaata atcagcgatc agttgtaatt ttttctctag ttttaatggc tctttgtcaa
     1201 taaggagaaa aacaagtcgg gtcttttgtc tcggaaagct tcaaccaact cgtgaataaa
     1261 ggttacgcct ccttgaaagg cgtggaaaaa ccaagtaatc gatcaatcac caatttcgca
     1321 tttggtagaa catgtttggt gagctgaaag taggtggcgt tcatgtctgt aactaagaat
     1381 tctatttctt taggattagt gcagctaagg aaatagtttg ttaatcgagg aattttttgt
     1441 gtaggtaaat tttctcgatt aatttttctg tctcgccatc cgcggaaata aaactcagtt
     1501 tatcctcaac ggaaacgtga gaacgaaatt cacaaccctc aacactcaag acagaattct
     1561 tttggaagac tttggtaaat agcttctcaa ctctttcaat gtgcgaataa cggttgtcaa
     1621 agatacctaa caacttttcg aaataaaaaa taacgacact ttttcagtca gtaaagaagt
     1681 gattttatgt ctgacatgat ttgaaatcga gtgccttgga cggataaaat aactttgagc
     1741 gttccaatgg cttcgacagt ttttacaggt atagcgctgc ttttttaggc gtataaccat
     1801 tggcatatga ttatattatt caaagtggac aatggtttcc ttcttcttgt ttttcaccac
     1861 cattaatttc ctatttccat ctaccacagc agaaccacaa tttctacaga cacatggagc
     1921 agggaagaga actgcagcaa cgatcaatgt ttttttcttc tgtaaggttt cgtaagagac
     1981 ctctgtaatc atcaaatttt tgtctattga tctcagaatt tttttgatag aaccattcat
     2041 aaagcatatc gttctctcag ttgtttattt tgtggtgatt taatcataca agaaaacgat
     2101 atgttttttt ataaaaacat gaataggaaa aatagttgac aagaaaatga caacgccata
     2161 tgatgttcca tagtatttac aacctgtcat agatgactgt aaaaaataaa agtaaaaaga
     2221 aaggacgaca acaggtggat ttttatcagt ctctacaact agatccgttt attttaaaac
     2281 aaaaaatacg agaagcagct tctaaaaaag aaaagtgcat gtatatttgt tcattgtttt
     2341 tacgatcatt attcatcgta ttgtttgcca tttgttttat cattataatt acaacattat
     2401 ttgaatcgaa gcataaacca tacgctgtgg ttttattctg tatgttgatg agtattcgtt
     2461 ttgttgactt tggttataag atttcccatt cgatcatagg tttagcaatc gtcatgttct
     2521 cactattagt agcaccgtat gtccagctga tcaaatggtc agttatgggc atgttgattc
     2581 attttatttt actaagcagc atattaatgg caaccgctag tgatcccaaa atggggaatg
     2641 cttctttgta cggcttttca tatctcttta ttgtttattc gttacctaaa gattcgttga
     2701 acaagaattt ctttacacaa acaagtagct tattattttt gtttttttgt tggttttcag
     2761 ttctccttta cagaaaacat cgagaaaaga acaaagacaa aagcttgttt aaagagattt
     2821 ttttgaaagg catgtattcg caggaaaaaa tttggatgct catctatgca tttggcatta
     2881 gtcttttgat cgttgcagga gagtatgtcc cttttcagcg attgatgtgg gcagggtttg
     2941 cattttcttc tattgtatct agctatggat tgatgtcgat tggtttcaaa gaacgagcag
     3001 tagaccgaat cattggctca ttgattggat gtgttttatt tattggtatc agccaattta
     3061 ttccttttaa ctgggtcggt atattaggtg gtctagcttt aggtgtttgt tctacctata
     3121 gatataaaac agtttttaat tcttttggtg ctttggcaat cactgccagt ttattcgggg
     3181 ttccaggagc agtaacgatc cgcatttttg agaatatcct tggggtatgt ttaggaatca
     3241 tgtatattgg ggtgactgaa atacttatcc gaaaaataag agaaaaacac ggtttgaatc
     3301 attagatgag atcgaataaa aaataaggaa aaagtgaaga tacacatgga tgataaattg
     3361 ctatcattcc atgtgtatct ttttacggta aatcagtgaa acgttcgact ttcattttta
     3421 atttatactt tttacggagt tcgtttattt gagccatcat ttcggatttg tgatgctttt
     3481 cgattgcttc ttcatctttc caacggtcga tcagtaaaag agattctgga tcttctagtg
     3541 gaaagtaata gtcatatttc tcgtttcctt cttctgcacg aatcgctttg acaatgtttc
     3601 tttcgaccat ttcttctgca aattttctgg cttgtccatt tgtacttgta tacaaaatat
     3661 tcactgtgat actcactaaa tcccctcctg aaaaaattct atcactagta tatcacggtg
     3721 ccagaaatta ttattcaaga tatgatacac attttttccg caatgctacg gaaatatata
     3781 ttttgaatag aaaacgctta caagatatgc ttggtttata aaaataaaag gagtgattga
     3841 tttatgaaaa aagcattgat tatttgtgcg gcaggaatgt catcctcgat gatggcctca
     3901 aagacaacgg atttctttaa gcaaaaaaga gaagaggtac tcgtagatgc agtttcagct
     3961 acagaaggcg ataatatgat taagaatagt gattttgatt tatttctgat cagcccgcaa
     4021 acgacgatgt ttttggataa attcaaaaaa ttaggagaat ctgttggaaa gcctgttgtt
     4081 agtatccctt tccaagcgta tgtcccaatc cctacaggca tccagaagtt agcagaggtg
     4141 atcgaagaga acatatgatg gaataggagt ggatgataat gaaacagaag gaaaaattgc
     4201 tgttgagata tttgattgat cacaagaagg agtatgtcac cagtcaacgt ttagcttctg
     4261 aattgttatt atctgatcgg acaatccgta attacttgca gcggatcaaa gagttagtag
     4321 aaaaaaacgg aggtaagatc attgctaaac caggttatgg ctaccaactg catattttac
     4381 aaagactgac atttgacttg tttttgagtc ggcaagaaat agtacctgga tatgctagag
     4441 aaccgcaagg attttatgag tccgaagatc gacaaaagta tatattgaac caattgcttt
     4501 tggaagatgc cattcttttt atggatgatt tggcagaaga gctttatatc agtcgttcca
     4561 gtttgacaaa ggacatgcaa gaaatcaagg agcgattgat tccatattca ctaaaaatcg
     4621 tttctaagca tggtcaaggg acatggatcg aaggggaaga aagaaatcga cggcatttta
     4681 tcatggatac tttttttgga aaccattatg gaaattcatt gaaagaatat ttagggcaaa
     4741 gccgtttttt cccagacatc agttttgaag agctagtcat tatcattctc gatgaaacaa
     4801 gagaagctaa gttaaaagtt tcggatttta tcatacagaa tctgacttta cacttggctt
     4861 tggggatcaa acggctccga gaagggtttg aaatcaagga attaggcatc gaacaagaag
     4921 tttctaagcg agtagagtat caagtggcgc agcggatcgt tcaacgtatt gaggtaatcg
     4981 caggtgtatc gtttcccgaa gaagaaattg cttatttgac attacatctc atggctaagt
     5041 cgaatcacaa agaaaatcaa aatgatcagg aactatcaaa ggaattagcg aaagttctga
     5101 aaagattgtc tcaggaattt catttttcat tagtagagga tctccagctt ttcaatggac
     5161 tactcgatca tttgtcaccg atgatgattc gattgagccg aggtattcgc attgagaacc
     5221 cattgactga ggagataaag aaagaaaatc ccaaagtctt tgacgcagta aaaaggcatt
     5281 tcagcaacat gccggctttg aaaaactata ccatcaatga agatgaatgg gcctatttgg
     5341 ctttgcatct catggcggca ttggaaaaag aaagagctgc gcataaacta catgcattga
     5401 ttatctgtgc aacgggttac ggcagtgccc aactattaaa gaatcgcgta gtcagtgaat
     5461 tcggtaaaaa tattacagtg gtaagtgtaa aaggctatta tgagatcaat gaaacaactt
     5521 taaaaggaat cgacctgatt atttcttcta ttgatctttc gacaatgttt ttcaaaattc
     5581 cagttttaca tgtcagtatt tttttgaatg aagatgatgt tcgtaaaata cgaaaggtag
     5641 ttggagaatc tataccctat cattcctcag atagaccgat accagcattt tctctacagc
     5701 aaaaaaagca gatttacacc gaacagttgt ctgaaaaatt tttcaagact tatacgtacg
     5761 aaccgaaaaa ggaagaaatt cttcatgatt tgctgcaaaa gctgagcata aatgaagaag
     5821 aaggatatat aagagaaatg cgaaagcaga tccagcaacg tgaaaatatg ggacaaatca
     5881 tattcagtga tacagtggca gtcccacatc ccgctgttcc agttggaatc gaaacaaagg
     5941 tagccgttgc attgattcct tctgggatgc agtgggatca atatcaagaa attcattttg
     6001 tttttttagt ttcgccttct tatgtcgaaa atgaaggaat tacggtcgtg actaaagcaa
     6061 tcgttaggtt agttgaccag ctaaaagtac agcaggaaat cttagctgaa cctatttttg
     6121 agaatttcag cagtaaattt atcaaactga tcgaataagg aggaaaacag atggctgaaa
     6181 aaataaacag tgaaacacta caagtgactt cttttgatat cattttccat agcggaaatg
     6241 cacggacact gatacatgaa gcatttcgct cgatgcgtaa agagatgttt gaagaagtag
     6301 aagagaagtt gcgacaagca aatgaagaga ttcttcatgc gcatcagtca caaactcaac
     6361 ttctgaaaga atatgccagc gggacaaaaa tagaaatgga aattatcatg gtccatgcac
     6421 aagatcatct gatgacgacg atgacattat tggaagttgc acaggaaatg aattatttgt
     6481 atcaaaaaaa taaatagaaa ggaaggatca ttgtgcagga tacagtaaaa aggaaaatcg
     6541 aattagaaaa aaagcaactt agtttgaaaa acatgtattt caatcgttat ctaatcgtac
     6601 gttacctgac tgcctttttc tttttcatga acatgcaatg gatgattctg cttctatcag
     6661 ctaaaagttt aggaagtagt ttaccgatga ttctgatttt agctattttg cctgcagtag
     6721 gagaacaagt aaagctttat cgaaaacacc aaacgaacgt tccatggaca aagtggtatt
     6781 ttttgttcca gagtgtcttt aatttattag tgattccagt gttgtttacc tcaggattta
     6841 cattcttgta tccttttatg gcaaataaca atggaggaca gttatttgtt ttcattcttg
     6901 ttgtaagcgg tatttttgcc agtgcattta tacagtatcg tttaaagaaa atttcactca
     6961 accaagacca acagtatatc cgtattaaac aatatgaaaa agcgttatat ttaggaaagg
     7021 agaataacta atgtttgatt ttttgcagaa atacctgatg ggacctatgg ggaaaattgc
     7081 gcaacttaaa atcgttcggg ctgtcatggc agcggggatg gcttctattc ctttcacgat
     7141 tgtaggctcg atgtttttgg ttttaaatat tttgccatta ccgttcccat ttttagaagg
     7201 attttttaat gcaacttttt tcaaagttag tgacttgtac atgatcgtca atacaatgac
     7261 gatggggatt ttatctattt actttgccat tgtgtttgct tatgaattaa cgtcaattga
     7321 acgagatgaa caaaaattaa atgttaatcc gctcacgggg gcattactat ctgtttttgc
     7381 attctttatg tgtatccctg aattgatcat gactgatggt aaaatcagct tgattgcttc
     7441 tatcaatgag acagaaactg tagtaaatgg tattaggatg ggctcttttg tcgaacgttt
     7501 aggaacttca ggaatcttta cagctattat tatgtcgatt attgcggtcg aattgtattg
     7561 tatgtgtgtc aagagaaatt gggtcataaa aatgccagat gttgttccgc caggagtttc
     7621 tcgttctttc actgctctga tcccaacatt tgtcattgcg tttgtggtga tgatcatcaa
     7681 tggattatta gttgcactag gaacggatat cttcaaaatg attgctatcc catttgggtt
     7741 tgttacgcag ttgacgaata catggcttgg tgtcatggtg atttatttct tgatccatgc
     7801 attgtggatc gtagggatcc atggagcgaa tatcatcact tctttcttga ccccgatcgt
     7861 attagctaat atggcagcta acgcacaagg cgcaaattat ccgttggcag gagaattcaa
     7921 caattcttat gtaaccgttg gtggatcagg tgcgacttta ggattgatca tctttattgc
     7981 attcctcgcg aaatctgatc agctgaaagt gttaggaaaa gcctcacttg taccaggatt
     8041 attcaatatc aatgaaccaa tcattttcgg gatgccaatc gtctacaatc cctatttagc
     8101 ggttccgttt ttcttagctc cgatggcttc tgctacactc gcttattttg ccattcggct
     8161 ggaaatcgtc aaaccgatgc ttgcacagat gccgtggcct tcaccagtcg ggatcggagc
     8221 atttgttggt agtggtggtg attggaaagc tgctgtgtta gcagtcattt gtgcaatcat
     8281 cgcattcatt atctggttcc catttatcaa attctatgat aataaattat taaatgaaga
     8341 aagagaaaag gcagcagaat tagctagtga aaagttaaaa tgatttctaa gaaaaaagga
     8401 actgtgacaa aaaatcacag tccctttttt tcttagaaat gggaaaccac tagtatataa
     8461 ataccattat tcttcaatca accgcttcac gattgcataa tccccttcat aaggttcatc
     8521 tacaccattg attttttgat agggatccac gccttttccc gcaataacga ctgaatcttg
     8581 cggagcgctt tctgctaaag cgcgtcgaat cgcttcagga cgatccattt cataatgaac
     8641 gatcaaatct ggattagtaa ttgcttcatt gatttcttcc gcgatttttt tcggatcttc
     8701 aaatgctggg tcatctgccg tcaaaaaggc gatgtcagct gtttctgata gcacttttcc
     8761 aaaatcatgt cgacgggaga ttgctttgtt tcctgggctg cctaatacaa caattacacg
     8821 gccatctgga tgttcatttt tggcaaattt cagcaatgtt ttcaagctta gataattatg
     8881 tgcatagtcc acatagacat gcgctccatt ggattggatc aattgatcca ttcggcctgg
     8941 tacacgagct tcttttaatc cgtcttgtgc atcttgactg gaggcacctg ttagtgctgc
     9001 ggcaatgatc gcactggctg cattttcttg gttgaatcct ccagccagca aaatatcata
     9061 agcagtatcg cagatagcta agcgatcttc atgagaagat aaagtaaaac cgtgagtacc
     9121 tttatctgaa cggatcactt gataattgct ttcttccgaa cgaccataag tgatcgttgg
     9181 aatctggaat agatcggctg tttccctaag taaatcaaaa tagtcacttt catgatttaa
     9241 aacaactgtt tttgaattca agatcaattg tcgcttgcaa tagaaatagt catcaaatgt
     9301 cggatgttcg atcggactga tatggtcagg cgagatattc aagaagattc cgacatctaa
     9361 agtcaagccg tatacgcgtt gtgttttgta agcttgtgaa gaaacttcca tcactagatg
     9421 agtcatacca ttttcaactg cttctgacat catacgatat aaatctaatg actcaggcgt
     9481 tgtcaaatgg gatttgaaat atgttttgcc atctaacgtt gtattcatag tagaaagcaa
     9541 tgctgttttt ttatttgttg tgtgatccaa gattgctttt gtaaagtacg cagcagttgt
     9601 tttacctttt gttccagtga atccgatcac tttcaattta ttttgcggat agtcgtaaaa
     9661 agccatagca agaagcgcca ttgctttacg gatatccgtt acgatgattc ctactgctga
     9721 attttcgtat ggttgttctg atacataata ttgaataccc tgttcgattg ctgaatccaa
     9781 atattcttct ttgaaattca ttcctttaca gaaaaacaaa gtagaagcat cagcttttct
     9841 tgaatcataa cttaactgaa gaaattcagc tttttctggt acatcaagat gccagcctct
     9901 tgttgaaaca aattctttta atagattttc ttttaatagt aaatgacgga tgtcttctaa
     9961 agagaagctc attgattgat ccctcgcttt attcaatttc ttggtttatt atagcacact
    10021 ctttttaata tatcgtttgt ttccgctatg acaaaatcca gctttattca aattttcttg
    10081 acacagggga caagcggatg agtattttca taaacaatga actgactgta atagatagga
    10141 gaacgaatag tgtctccaac aatccaaaaa cataataatt caaaaagacg ataagactat
    10201 tggtaagtaa aagtccttgc ggcattggaa gccgaaaaaa tttgtggaaa ataatcggcg
    10261 gtatcgttat accgccattt gaggccccga ctgtgtacaa tgtgtaaacc ccaagactga
    10321 acaaaagact tcctgaaatc agtgaaaaga aatgtgagga gatcatttcc acttttggaa
    10381 caatctctaa aacaacagga aaaagcagac ttcccgctac agtcttaaaa aatactgatt
    10441 ttcctaatag gacagcggct agaagcagca tcataagatt gatgctccaa acagtaactg
    10501 agcgttggat agaaaaggcc gtttcaacta aatgtccgat ggcgcccact ccggcggaag
    10561 ctatatcgtg agggagcaag aaccaattca atccaactgt gataaagaaa agacctaaaa
    10621 atataacggg tagttttttt atttcttgtg tcattgatat ctcctataaa tgttagccat
    10681 aaaaggctgt atatttcttt actttaccaa aaaaacgaaa ccgaattcaa aaaaacaatt
    10741 gttcttataa aagttatata aaatcgattg ccagtttctg ttttaccaat taaaatgata
    10801 gtaatgtttt gtagaaagtt ggaatctgac cgatggataa tcaaagaaaa ccagcattag
    10861 atcaattaac accgcaagaa aaaatggcac gaggctctgc ttggatgacc gccagtaata
    10921 tcatttcaag attgctaggc gctgtctata tcattccctg gtatgcttgg atgggcgaaa
    10981 atgctaaggc tgccaatgga ttattcaata tgggatacaa tatatatgca ttgtttctac
    11041 tgatctctac agcaggaata cctgctgcga ttgcaaaaca aactgcacgc tataattcat
    11101 tgaatgaata tggcacttca cgacgattat ttatccgtgc attacagatg atgggcgggt
    11161 taggcctgtt gtttgctttg ttcatgtaca tcgcctctcc ttggctagct catgcttctg
    11221 gtggcggaga agaactgatt ccaacgatgc gttctttgag tatcgctgta ttggtttttc
    11281 cttgtatgag tgtgatacga gggtatttgc aaggcaatca agaaatgatg ccttatgcgc
    11341 tgtcgcagat tgtagaacag gtggcacgta ttttttatat gctattggct acattcatca
    11401 tcatgaaagt catggatggt gactatgtga cggcggtcac gcaatcaact ttcgcggcat
    11461 ttatcggtat gctagcaagt attcttgtcc ttttgtattt tttgaagaaa catcaagcgt
    11521 atacatcggc gtttattcac tacagcgaaa acaaaataaa tattgcaacg aaggaattgt
    11581 tgttagatac cgttaaagaa gcgattccgt ttattattgt gggttcaggg gtaacgatct
    11641 tcaaattagt cgatcagttc acttttatga agatcatgtc tgcatccacc gagtattcca
    11701 atgcacagtt actggatctt ttttcgattt ttagtgccaa tccagataag ttgacgatgg
    11761 tagttattgc attggcaaca tcgatcgcgt ctactgggtt accgttgatt acagaagctg
    11821 ttacgttgaa agatcgagta ggtctggcaa aattgacaag cggaaattta cagttgtttt
    11881 cattttttat gtttccggcg acatttggtg ttatgttact tgcctatccg ctaaatacat
    11941 tattttatac accggattct ctaggaagta atgtactgat ccaagcaagt tttgtaggac
    12001 tcttcctagg actatatatg ctagtttcca acatgctgca aggaatgttt gaaaacaagg
    12061 cggcaattaa ttatttggtt attgggtttc tagtgaaact ccttcttcaa tacccagcca
    12121 ttcgtttatt tgaagtatac ggccctttat tggcgacaat gatcggattt gctgtgtctt
    12181 gtaccttgat cttaaagaga atccatgaag tagcccgctt taaaccaagt tttgtctggc
    12241 gcaggacatt gctgatcttt attcttacct tgattatgct tttggcttcg tggatcacaa
    12301 agatgatttt tggtgctttt ttaaacgaag atagcaaatt ccagtctcta ttgttgattc
    12361 ttgtagtagc aggtataggg gctttagtgt atggttattt agctttgaaa atccgcttgg
    12421 cagaaagact actaggacca ggaatagcgc gtttacgaca taaattaaaa ataaaataag
    12481 tgaatcagaa gagctagatt gatgctcttt tgaaatgggg gaaacgcatg cgtttagata
    12541 agtttttagc agatatggga cttggcagcc gcaaagaagt caaaggatta atcaaaaaag
    12601 gaacgatcga agtcaatgga caagtggtga aaagcgataa agcgcaagta aatgaattcg
    12661 aagatgcggt tacttattta ggagaaatca ttacgtatca aaaagatttt tactacattt
    12721 tgcataaacc tgcaggagtc atttctgcta ctcaggataa tcatgatcaa accgtaatcg
    12781 atttattgga agaccaggat tatcgggaag atctttttcc agtcggccga ttagataaag
    12841 acacagaggg cctcttgatt ttaacgaatg acggggtgtt tgcccatcag ttactatcac
    12901 ctaaaaaaca tgtggagaaa gaatatctag ctgaggttag cggtataatg acagaagaag
    12961 atatccgttt atttgcagaa ggactgatta ttgatggcga tgagcagaca ttgccggcaa
    13021 aactccaaat tgatcaagta gatcaagaga cagaaacttc aattatccgt ctgattttgc
    13081 atgaaggaaa gtttcaccaa gttaagcgaa tggtcaaagc agtaggaaaa gaagtcttat
    13141 atttaaaaag aatccgtatg ggaaaatttt ggctgcctaa agatttgaat aaagggcaat
    13201 atcgacccat gaccaaagaa gagctagaac aagtgagaga aaaataattg aatggatagc
    13261 taaaagaata agcagagtag ttgtagcgcc atctgaaaag cagacttgtc cgtctgactt
    13321 tcggatggct tttttgtgaa atttcggctt atttccgtag aagctacttc tggaacaccg
    13381 tttgttcgga ataagggact gtgacaagac ttttgttaca gtccccattg ttccttgtga
    13441 ctcagagtta gatagtaagg acacgatctc cctaatactc aggccaaaac gctgaattca
    13501 caacctctga taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa
    13561 aatatgagga actattttcc cgtttttttc tctaatactc aggccaaaac gctgatttca
    13621 caacctctcc tatactttgt ttcattttcc ctatactttg tttatttatt ctcattattg
    13681 aaagcgctat aattacgttg cgaatgaatc gaaaggggtt tttagatgaa aaaacgaaag
    13741 gggttcaatc atttttttat gaatctatgg cgttataaag cacttgtcct gatggctgta
    13801 ccgggtatgc tttggatgat ttttttcttt tacatacctg taatggcgaa tgtcgtagca
    13861 ttcaaaaatt ttcatatttc agcagatggg ttcttggcta gcttgaggga aagtccttgg
    13921 gtgggttggg aaaatttcaa atttttattt tcttcggatc aagcatttct catcactaaa
    13981 aatacaattt tatataatgt agcatttatc cttttgaatc tgctgatctc agtcgtattt
    14041 gcaatcatta tgagcgagtt gagaaataaa cgactcgtta aagtttatca aacgatgtcc
    14101 ttactacctt attttctatc ttgggtcatt atcagttact ttgtttacgc ttttttaagt
    14161 cctgacaaag gaatattcaa tcagtggatc actggacatg gaggagaagc tgtcaattgg
    14221 tataatgaac cgaaatattg gccgtttatc ttggtgttca ttggtacatg gaaagggatt
    14281 ggttataaca gcattattta ctttgcatct gttatgggaa tcgatccgac ttattatgaa
    14341 gctgctatgg ttgatggagc tagcaaatgg cagcagatca aacatgtgac gataccacaa
    14401 cttattccac taatgacgat tttaacgatt ttagccgtag gaaacatttt ccgagctgat
    14461 tttggcctgt tttataacat tcctaggaat tccggtgcat tatatgatgt gactcaagtt
    14521 ttggatacgt atatctacaa tggcctgaca tccacaggag attttggtat gacggctgct
    14581 gcaggacttt accaatcagt tgtcggcttt gtcttgttga tgatcacaaa tacgatcgcc
    14641 cgacgttttg atagcgattc tgcattattc taaggaggaa aaaaatgaaa aaaaagaaag
    14701 tggagagagt ccaagtccgc tctttcaata agacggctaa ttttttctct aatataatga
    14761 ttgcactctt tgctatttcc tgtattttgc catttatatt tgttatcgcc atctctttta
    14821 ccgatgagtc catattgtcg actcacgggt acagtttttg gccagaaaag ttcagtatat
    14881 ttggttatac gtacttgttc gaccagatgc aggataaaat tttccaagcg cttttcgtca
    14941 ctgtacttgt tactgtttta ggtacactga tcaatagcac ggctacttct ttatatgctt
    15001 atgcgatttc acggtcaaac tttccattcc gtcgtttttt taccgttttt tgtttgatta
    15061 cgatgttgtt ctcaccaggg atggtggcca attatcttgt tatgacaaat cttcttcagt
    15121 taaaagatac gatttgggca ttgatcttgc caatggctgt cagtcccttc aatattattg
    15181 tgatgcgcac attctttaaa cgttcggtct ctgattcgat tattgaatcg gcacggatcg
    15241 atggagctag cgaactgcga atatttgttc agatcgtttt accgttagct gttccaggta
    15301 ttgcgacaat cagtttattt gccgcattgg gttactggaa tgactggttc aatgctttac
    15361 tatacatcca agatgataag ctagttccac tacagtatct tttgatgaaa atccaaagta
    15421 atattcaata tctgacacaa aattcaggag caggaagcca attgacaggc ggattagcct
    15481 cagttccagg agaatctgcc agaatggcga tcgtagtcat ctcgacgttg cctattgcga
    15541 tcagctatcc atttttccag aagcattttg tcaaaggtct aacaatcggc ggagtaaaag
    15601 aataaagaaa caaaaatata aatcataaaa ttaggaggaa gaaaaatgaa gttatggaag
    15661 aaactagcct ttacaggtgt ttcagcgttg ttgttgggaa cattagcagc atgcggaggt
    15721 tctggttcta aagaccaggc agaagctagc aattcggaca ctttgcagat gtatcaaatc
    15781 ggagataaac cagataattt cgatcaattg atggaagtag caaacaaacg aatcgaaaaa
    15841 gagatcggtg taaaagtcaa tatcaattac attggctggg gcgattacga aaagaaaatg
    15901 aacgtcatca tttcttccgg ggaaaattat gatatcgcct ttgcgaacaa ctatgtttcc
    15961 aatgcccaaa aaggagcctt tgctgatttg actgaattag cgcctaagta cgctgaaaaa
    16021 gcttataatg atttagatga agcatatatt aaaggaaatc tggtcaatgg aaaattatat
    16081 gctttcccag tcaatggaaa tgtctttgct caacaagtac ttacttttaa caaagcttta
    16141 ctagataaat acgatctttc aattgatgga atccaatctt acgctgatgc agaaaaagtc
    16201 ttgaaggaat tccatgaaaa agaaccaaac acggcagctt ttgccattgg acaaggcttc
    16261 aaagtccaag ggaactttga ttatccttta gggaatacgt taccatttgc tattgatctg
    16321 gatggagatg attcgaaaat catcaaccaa tacgataatg aaacgtatat cggactgtta
    16381 cgtacaatgc atagctggta caaacaagga ctcattccaa gtgacgctgc aacgagcaat
    16441 aaggattatc cactagaagg aaatacttgg ctaatgcgtg gagaaacaca aggaccgtat
    16501 gattatggcg atacaatttt gacaaatgca gcaaaacaag aacttgtctc aaaagcaatt
    16561 actgttccac taaaatctac agctcaagca caaatggcaa attttgtcgt atcaaatact
    16621 tcaaaaaata aagaaaaatc agtagaactt ctagggctgc ttaacagtga tccagaatta
    16681 ttgaatgggc ttgtttgggg gatcgaggga gaagcatggg aaaaagtcga tggttcagat
    16741 gataaaatca aattgctaga tggttatcag ccaaatacac atatgtctgc ttggaataca
    16801 ggaaacaaca agattctata tacacaagag tctattacgg atgacatgat tgccaaacgt
    16861 gatcaatcga ttgctgatgc agaaacttca ccgattttag gattcagctt caacactgac
    16921 agtgtcaaaa cagaattaag taatatttct aatgtcatga atcaatattt agatggattg
    16981 aatacaggga ctgttgatcc agatgaaaca ttaccgaaat tgaaagacgc tttggacaag
    17041 gctggctatg ataaggtatt gaaagaaatg caaaaacaat atgatgagtt ccgacaagaa
    17101 taataatgaa taaagacaaa ggggttgtga caaaagacgt gtcacaacct cttgttccga
    17161 ataaacggtg ttcaaaagcc agtttctcgg tattaagcta aatatatgta aaaatgtgga
    17221 aagccatatt cacaaaattt ttcttactac tcgaaactaa gcggctattt cacaaactct
    17281 tggtaaacgg tgttcaatca gctaaccctc ggtattcgct cacaaaacga caaaatatga
    17341 ggaactattt ttccgtttgt ttctctaata atcaggtcaa ggtgctgatt gcccaacctt
    17401 tttttgtata atgagaaagt ataaacttaa tgtttggagg agaagatatg ttagggaagg
    17461 ttttggaaga gttatttatc cggatctggg tcattataaa actaaccttg tatttctgga
    17521 tatatacatt tgccggcggg atcatctttg gattaggtgc ggcatggaaa acagtcaatg
    17581 aactatttta tttgtacgga ttcgagtata aagaaatcac aataaaaaga ggctggaata
    17641 tatataaaag aaatttttta agaggcaatc tgctatttag tttattttta tctggtactg
    17701 ctttattgag ttacaattta tattcatctg ttcaaatcaa agggctcatt tttttagcta
    17761 ttgattttat tttacttttt gccttgctct gcctatttgc tacttatcaa tacagtatga
    17821 ttttagatag tgaatatacg atctctgttc ctaatctgct aaagctaagt tttatttcgg
    17881 ttttttccag cttttcgtct tttctaaaaa taattattgg tagcggtata attttaggcg
    17941 tgacttggca attcaaagga ttgatcctgt ttggtgtcat tggtcttttg acggtttgga
    18001 atggtacgat gacaacacat tggcgtgaag aattagataa acagttggaa agttatgaat
    18061 aagtttcgga ttctattttc aaaaggaacc ttactaaatc gtctgttgcg gaaatacgcg
    18121 gttatcatga tcggtgtaac gatgactgct accgttattt ttagtatcca tacttgggaa
    18181 caaaatcaaa aacaggcaga aaacatgaca agtgatgccg ttcaaagcac aagcagaatg
    18241 ctgaatgata aaacaacctt gagtaggatc atcaaaaacc agcttgtagg gaattccgaa
    18301 aagatcgaaa atgtgacgac ctacttaaca aaacccatag atcagtatct gatgtatgta
    18361 tacgaacagc aaaacagtac cgatgaatta gtttctttcc caaatcaaat caaagatctc
    18421 tacgcaaatt atgaagaact aagtgcgata tatatcgtgt tgaatcaact accggagtat
    18481 tatgaatctt ctagagaaaa caaaggtggg gaaataaaaa aggggacacc aaaattacta
    18541 gacgcctttt atatcaagtt gcctatcaca caaggaggag ctgagtcagg aagtattttt
    18601 attggttttc aaaagaaaga actagatatg attttagaaa atctaacgtc attcaatgga
    18661 ctctctttat acatgatttc tggaacgaca aatcgactat acacattcca tgataataaa
    18721 atagctaaag aaacgtttga aagacaagaa atgatcatca cagagagtct gaagaaaaat
    18781 tcacaattgc cgattgacga tctggaaaaa aacaattttg ttcaacacca agaactgtct
    18841 gatgattatc gtatattagc tgtactagat aaaaaaagtg taaaaatcga aacgggaaaa
    18901 aatcttgccc cgctgatcat tggtatcatt attttagaca atatactgct tatctttctt
    18961 tataaaacat tcaaacgata ttcacagcaa gtatctatcg tcatgcaggc catggatcaa
    19021 acagcagcag ggaatttaga gacgcggatc gacataacaa acacggaata tgaactgaag
    19081 gaactttcaa tcggtatcaa cgaaatgttg gatagcatca atcagtttgt agaagacatc
    19141 tacaaattag aaatcaaaca gcaagacgca catatgcggg cgcttcaagc acagatcaat
    19201 ccacactttt tatataatac attagaatat atccgaatgt atgcaatcag cgaaggaagt
    19261 gaagaattag cagatgttgt gtatgcgttt tccgctttgc ttcgaaacaa tacgaatcaa
    19321 gagaaaacga tcactttaaa agaagaactg gacttttgcg aaaaatatgt ctatttgtat
    19381 caaatgcgtt atcctaatcg agtagcctat cattttatga tcgatcctga tttggaaaag
    19441 atcgaagtac ctaaatttgt gatccagcca ttagttgaaa attattttaa acacggtatt
    19501 gattttactc gattcgataa tgcgctaagt gtcaaagtct tgcaggaagg caaacgggta
    19561 aggatcatca tcaaagataa tggaaaaggg atgactgaaa aacggctaaa acagattgaa
    19621 gaaaaactgt ctcatcctaa agtggaactt cacggttcta tcggtctgca aaacgtgaat
    19681 gaacgtttac gtgccagctt cggcagcagt tattatatgt cgcttgagaa taatgagaca
    19741 ggtggactga ccgtttctat tacattcaag gagggatgag cgtgtatcaa gtgttattgg
    19801 tagatgatga atacatgatt ttgaatgggt taaaaaagat aattgattgg caaagtttag
    19861 gctttcaaat agtagcaaca gcagagaatg ccaaagaagg actagctgta cttgaacagc
    19921 gacagattga tttagtagta acggatgtga caatgccaga aataaatgga ctggaattta
    19981 ttgaagcggc tcaaaaagaa cgtcataatt ttgaatttat gattttatca ggataccaag
    20041 aattcgatta tttgaaagga ggtatgcaat taggcgccgt gaattatttg atgaaacccg
    20101 tgaataagtt cgaattggtt gaaagcctca agaaaatcaa aacacggtta gatcagcaaa
    20161 atgaacaaaa aaatcaacaa gaaatctatc aagaactgtt attcagtcaa tggttgaatg
    20221 aagaattgga tgaaataagt gaagaagaaa taataggaca aataagtgag aagcagcata
    20281 ggatagtatt gattcaactg gcaagaactc atgatactct tatcaataga tggttgcaag
    20341 aacggcaaca aatcttttac tatcaacgaa attacggaga ccgtgtattg atcacgttgt
    20401 tgttggaggc agatgaagtt gatggcttct gttgttttgt tcaagaaaac tttcctgatc
    20461 aggaatggct gatcagtatc ggggaagaga cagattcttt ggacaaaatc cctgaaacat
    20521 tccaacaagc gaaagacaat ctacagttgc atcaatttta tggagataaa gacgagcatg
    20581 ttttttacgc agtacaagca gaaacaaaag atcaaaccat cgatttttca agtttcagac
    20641 gcgtgctgca aaataaacaa ttggaagtcg ctcaaaaaat gatcactgat ttttttgaac
    20701 agtttcagtt agcagccatg atgccggaag atatacgata ttccagtttt ttactgttta
    20761 tggagattca acgggaattg attgatctgg aagatgagga atatttacaa ggaatcgaaa
    20821 aaatccatca ggcaaaaacg gtgcaagaac ttcagcagct acttctgtca ttcatcaaaa
    20881 agcatcagag acagaaaaag tacagtgaca atgtggaaaa agtaatcgta atcctgcacc
    20941 agcattatca agaaccactt acactcaaag aagtgtcaga aaacctgcat ttaaatgtca
    21001 tgtatctagg acagctgttc aaaaaagaaa cgaaaaaaag tttttcagct tatttgaacc
    21061 atttacgtat ggaaaaagcg aaacaactgc ttttgcacag taatcaaaac atcaatgaga
    21121 tagcaagtga gattggctac aacaacacca cgtatttctc taaattattc aagaaaatcg
    21181 tcggcagatc ccctaaagag taccgagaaa acagtggaga atcataaaag aaaaagccaa
    21241 tgtaatgaaa taacttataa aaaattgtct agaaatctaa ttttgggctg ttcttattac
    21301 ttgaggcaag gcagcttttt catgacctct cgataaacgg agttcaaaag ccagtttctc
    21361 ggtattaagc caaatttatg caaaaatgtg gaaagccatt ttcacaaaat ttttcttaat
    21421 actcgaaact aaacagcttt ttcacgacct ctccactaaa cggtgttcaa tcagctgacc
    21481 ctcggtatta gctcaaaaaa tggcaaaata tgaggaacta ttttgccatt tgtctctcta
    21541 atactcaggt cagaacgctg atttcacaac ctctcaataa acggtgttca aaagcttgca
    21601 tctgcggtaa taagaccaac caaacgaaaa atatgaggat ctatttttgc ttggctgttc
    21661 ttattacttg atgcaggaca gctttttcac aacctcttgt taattatagt ttgataagga
    21721 aaaaggcaaa tcttttgctt gatatttctt agggtgaggg atgatcccta attggtaaga
    21781 gatcgtgccg cctttcagca aatcttgatg ggtaaaatat aatttcgtat attctttgtc
    21841 catccgtttg attttatgga taaattgttg ctgcggccga ttaggcgttg cttcaatcgt
    21901 caattccttt ccatttgaca aaagcaaaac agccttgtca attaatggca taccgataac
    21961 atattcaccg gttccaggcg tgacaggata aaatcccaga ctattgaaga tataccaagc
    22021 ggccatactt ccattgtcct catctccagg gtaacccatt ggagaatcgt cgaaggtctg
    22081 agtcatcaac tgtttaagca gtggctgtgc catttcaggt tgtcctatat aactgaacaa
    22141 gtaaggatag tgaaaacttg gctggtttga aatagctaat tgtccaaatt ctatcgcggc
    22201 catctcactc atttcgtgga tctcaaaacc atatcctcct gggtcaaacg ttgggggctg
    22261 attgcacagc tcaatgagtt tttctttata tctctcactg ccaccatatt ttccgatcag
    22321 tcctgaaaaa tcgtgataaa cagcaaagct gctttgccaa gcacttcctt ctgcataatc
    22381 ttgtccccaa cgattagctg aaaatggttc tctgaacttg ccatctttat cttttgaacg
    22441 cataaatcct gactcatagt caaagatatt ttgatagttc aatgattgct gctgatattt
    22501 cttcgtcatt tcctcttctt ctagtacctt ggcaacttgg ctaatacaaa aatcgctata
    22561 agcatagtct aatgtgtgat tgactgattc gtggtagtga ttaggaacat agccatatgt
    22621 caagtaatct tctgttcctt ggcgtccata atttggatgt ttgctttgaa ccgtagcacc
    22681 tttttccatt gccgttagaa attcaggcat caagtctcgt cggatatctt ttacagcagc
    22741 atctgcaatg accgcatcga tcaatgtccc aggcataagt ccacgttcat caggagacag
    22801 ccattttggc aaatagccac tttctctata gctgttcaaa aaaccttcca gcatttcttc
    22861 gtatttttct acagcaatca aagaataaag aggataaacc gttttatacg tatcccaaaa
    22921 cccattattt gtatagagaa caccaggttt tatagatcga ctagtcgtat catagtggat
    22981 tttttgccca ctaggatcaa tttcataaaa tgtttgtgga aataaaaaca cccgatataa
    23041 attatgataa aacgtccgca attgttgttg atcatggtgt gtgagctgta tacgatcaaa
    23101 ataggactgc cattgttctt gactgtgagc aaggtaagtc tctttgcttt ggttttgttc
    23161 tagttccagg ttataagctg cttgttccga gctgataaaa gatgtcccaa atcggatagt
    23221 ttgttttttt gtctgttcaa aatacaacgg gatcgagtcg ctatttttgt tggaaacagc
    23281 taactcatca attgttaaag gatattcaaa tctcaatata aaataaaaag aaaaatcagg
    23341 atcttctgaa ccagcaaaat gtgtgatttt tccttccact gtgtaaggat ctataacaaa
    23401 gagctgatgg cgtcccggaa aggataaaag taaaccgctg tcatttttgc tgtaatcaat
    23461 agataagatt ccgccataca tgctaggtat caatgtagaa cagatgccat aacgtaattg
    23521 acagatcgac agatgagtcg ggcaaaatat actttcttct gggcggtaag aactttgagc
    23581 atggaaaagg gtgttctcct gtagttttcc attgatcggc gtaaacagca tatgactgaa
    23641 atcacccatc caaggacttg gttgatgggt aagacggtag ccttgaaaaa cgcggtcatc
    23701 aggatgaaac caccagctgc cgttttgatc cgtagtttgc ggtgcaaaat aattcatccc
    23761 aaaaggaacg ccagtataag gctggcagtt cccattagag aaatttggct gattcgctgt
    23821 tgcatgtcta gtatctattt gaccgatatt cattttattt ctcctttgtt gattgtcttt
    23881 tgatgaatga aactggaaat ttactatcgg atggtcgatc ttttgtctct aaccaagtga
    23941 ccaaagcatc tcccgcaagc ttacctaact gttcaaaatc ttgtgcgatg gttgtcagtg
    24001 gcgggtcaac aagagctgct gcttggatat catcaaatcc gataattgaa aaatcttcag
    24061 ggatatgata accatgctgt ctcaagagat tcatggtctg gatagcgaca acatcgtttt
    24121 cacaaacaaa agcagttact tcatacttag tgatataaga aagcaattca tctaatgtcg
    24181 cgcttttgtc atccagtgaa gtgtggaaag tcaaaccagc tttgtccaat gcttcaaggt
    24241 aaccaaggta acgctgtcga acggattgtg gatgagtctg cgtaccaaac aaataagcga
    24301 tttttgaatg tccctgttcg atcaaataag aggcagcaag cgcaccgcct tggaaattat
    24361 cggaaagaac ggtaggaaaa ggtaactcat agattttttt atctaatatg acgacaggga
    24421 attctttcaa tgaaagctca aaaagtaagt caagatgttg ttctgtatca agagcataat
    24481 aaatcaatcc gtcaaattca ttgattagct cttttgctgt tttattttgt agaaagtcaa
    24541 gcgtcgtcat catgacatca atctgtttat cttgcatcac agggttcaat cccttgctaa
    24601 aatcaccaac agacaaatca gcgacatatg gaagaagaaa cagaatccgt cgtgtttttg
    24661 taggagaagc aagaatctgt tccggagaag cggatttaac gaaactgcct ttcccgcgaa
    24721 cgcgataaat aagtcctaat tgctctaatt cagtcaatgc acgtttggaa gtgattctgc
    24781 ttactttata ggtacgtgaa agttcttttt cagttggtag ttggctgtct acaggtaatt
    24841 caccagttaa aatagccgtt tgcaaatcat taaatattgt ctggtacaat ggttgagtca
    24901 tataaacacc tcttaatgat atatcatatt caaataatac cataatggat gaaaaagtaa
    24961 ataaaaagat attttttgaa agcgtagaca taattggctg tatgaagtat aatctttaaa
    25021 gatatatcaa atagaaagaa ggttttatta gtggtttata aagatatacc aaaatcggtg
    25081 cagaaattta tggaagatat cacacaaaaa tgtggggata tccatgcgga ctgggcaaag
    25141 aattttacag cagcatttgc caatactctg ttgacaacag tcaaaaagca aaatgacgga
    25201 accacctttt tattgacggg agacattcca gcgatgtggt taagagactc tactgcacaa
    25261 gttcgtcctt atttagtgat tgcaaaagaa gatgaggaat tagctgacat gatcagcggg
    25321 ttagtcaaaa agcaattttt ttatatcaat atcgatccat atgcgaatgc cttcaacgaa
    25381 gaagctaacg gtgctggcca ccaaacagat catacaaaaa tgaacgattg gatctgggaa
    25441 agaaaatatg aaattgattc tttatgctac ccaatacagt tggcctatct tctttacaaa
    25501 aatacaggaa aaactgatca attcaatcaa tcttttattg aaggtatcaa aaaaatcttg
    25561 gacgtgttca aaacagagca agatcatgaa cactctcctt atacatttgt acgagacacg
    25621 acaagaaaag aagatacgct gatcaatgac gggcgaggtg caaaaacagc ttttacagga
    25681 atgacatggt ctggtttccg tcctagcgat gatgcttgcc gttatggata tctggtacca
    25741 tccaatatgt ttgcggtggt cgtgttaggt tatgtggaag aaatttttca aacgatactc
    25801 gatgataaag aaattgtaca gcaaacacag aaattgaaga aagagatcca agaggggatt
    25861 cagaatcacg gacgaacggt taatcaaaaa gggcaaacga tttatgcata tgaagtagat
    25921 ggaatgggaa acgccacagt catggatgac agtaatgtgc ccaatctgat cgccgcacct
    25981 tacttgggat attgttctga acaagaggaa gattatctgc aaacaagaag aacattgttg
    26041 agtaaagaaa atccatattt ctatgaagga aagtacgcaa aaggaattgg atcctcccat
    26101 acgccagaaa aatacgtttg gccgattgca ttagctatgg aaggaatgac aacgtcagac
    26161 aaatctgaaa aagagcggat attgaattta cttgtagcaa ctgacgcagg aacacacctt
    26221 atgcacgaag gattcgatgt gaacaatcct gaaaattata cgagagaatg gttttcttgg
    26281 gcaaatatga tgttttgcga attagtcatg gattactttg atattagagt agaaaagtag
    26341 gaggtatcgt aaatgaaaaa gaaagtatat atcatttctc atagccactg ggatcgtgaa
    26401 tggtacatgg cttacgaaca gcatcacatg cgactgattg aattgataga cgatctacta
    26461 gagctatttg aagttgatcc gtccttcaac agttttcatt tggatgggca aacgattatt
    26521 ttagatgact atcttcaagt aaggccagaa aaaagacagg caatccaaca agcaatcaat
    26581 gaaggaaaat tgagaattgg accattttat atcttgcaag atgattttct tatcagctcg
    26641 gaatcgaacg tgagaaatat gctgattggc atggaagaaa gccgtaaatg gggaacccct
    26701 gtcatgttag gctattttcc cgatacattt ggaaatatgg gacagacgcc tcaattaatg
    26761 aaacaagctg gtatttcagc agcagcgttt ggccgtggtg taaaaccaat tggctttgat
    26821 aatcaagttt tggaagcaga aaactattct tcccaatact cagagatgtg gtggaaaggc
    26881 cctgatcaaa cagcaatctt tggtctgcta tttgccaatt ggtatagcaa cgggaatgag
    26941 attccagtag aaaaagaagc agctcttgcc ttttggaagc aaaaattagc ggatgcagaa
    27001 caatatgcct ctacgaacca cttattgatg atgaatggtg ttgatcatca accggtacaa
    27061 aaagatatat caaaagcaat ccatctcgca aatgaactgt ttccagatta cgagtttatt
    27121 cattctaatt ttactgacta tctggaagca gttcaaaaag atgtgccaga agacttaggc
    27181 tcagtagaag gagaattgac aagtcaagaa acggatggct ggtatacttt agccaataca
    27241 gcatctgctc gggtttactt aaaacaatgg aataccaagg tccagcgaca gctagaaaat
    27301 attacagaac cacttgcgac gatggcttat gaagttagtg gaaactatcc ccatgatcaa
    27361 ttagattatg cttggaagac attaatgcaa aatcatcctc acgacagtat ttgcggttgt
    27421 tcagtcgatt ctgtccatcg ggaaatgata cctcgttttg aaaaagcaga tgaagttgga
    27481 aagtatcttg cacaagattc cttagaacag ctgaccgcag caatcgatac gaccggattc
    27541 ccaaaagaca gctttccatt tgtcattgta aatactgcag gaatggataa aactggtgaa
    27601 gcagaaataa ccatcgaatt agaaagaaaa agattcgctg aaggaatacc tgaacaacta
    27661 tatcaagagt tagaaaattt gcctaaacgt aagtatcatg tagaaacaaa aagtggggcc
    27721 actataccag ctattttatc tgaagaaact gtccaatttg gttatgattt gccgaaagac
    27781 cgtttccgtg tgccatatat ggcaagaatg atcaaagtga ctttaccatt agaaaacatg
    27841 ccagcatttt cttgggaaac attcgcgctt gtagaagggg aagcgaaagc agaagaaaag
    27901 gaaacaatga ttcatcagag tggacgaatc atcgaaaacg gccctctaca tttgacgatt
    27961 gaaaaaaatg gtaccattac aatggaagat cgcaaaaaca aacgaaagtt gaataatctg
    28021 catatttttg aagatatcgg cgatatagga aatgagtata tctttaaaca acctttctgt
    28081 gatcagccaa tcctatccag taacaaagaa aactctgaag taaaagtatt agtagataca
    28141 ccggaaatag caaaaataag tattctacag gaaatggaaa tccctgtttc tgcagacgag
    28201 cgtttagaaa aagaacagca gatggttgtg gaatttcgtt atcgaaaagc agaacgatcc
    28261 aaagaaaaac gaatccttca aataaaaaca atcatgacaa tcagaaaaga tagtaaaaaa
    28321 atcgactttg aaacgactct cgacaatcag atgaaagatc atcgcttacg tgtcttgttt
    28381 cctacaaagt tacatgtgga acatcacgaa gcagatagta tttttgaagt ggtgaagcgc
    28441 ccaaaccatg tgtctaaaag ttgggaaaac cccacgaatc cgcaacatca gcaagcattt
    28501 gtcaatattc atgatgaaga gtacggtgta acagtcggaa actttggttt gaatgaatat
    28561 gaagtaacag aagacggaca aatcgcagtg acgctacttc gcagtgtggg agaactaggc
    28621 gattggggat attttccaac accagaagca cagtgtctag gtgagcatcg gttcaattac
    28681 agcattgaac tacatggacc agaagaaaaa ttctctactt atttgcatgc atacgcagca
    28741 cagattcctt tttccactca acaaatcaaa catcatgaag gaacattgat ttcaaaacaa
    28801 caatatttaa cgataaaaag tgaaacgttt gcaattacag ctttgaaacg cagcaagttt
    28861 agtgataaag tagtagtaag aggtttcaat atgtctagtc atttagaaaa attggaaata
    28921 actaaagaca atggaaaaac tgtcatattg aatctgcttg aagaaccaac gaagcaagcg
    28981 gttgtcccaa taatccaacc ttatgagatt cggacaatcg gattcgagga ggaaaacgaa
    29041 tgtatggcgg aatagaagca ggcggaacaa aatttgtgtg tgcggtttca gatggtggag
    29101 aaattttaga aaaattaagt atccctacca caacaccaga agaaacattg gatcaggtat
    29161 ttgaattttt tgatcgttat gaattgaagg gaatcgggat tggctcattc ggcccgattg
    29221 gtatcgacaa aacaaaagat aattacggat acgttttggc aactcccaaa aaagggtggg
    29281 cacaattcga ttttcttggc acaatcaagg aacgctacaa tattcctgct gcttggacaa
    29341 cggatgtcaa tgctgctgca tatggggaat tgaagcaagg agcagcacaa ggaaaaaata
    29401 gttgtattta ccttaccgtg ggaacaggaa tcggtgcagg tgtcgtgatc aatgaagaaa
    29461 ttttttcagg gatcgcacat cctgaaatgg gacatatttg ggtgaaacgc catccagatg
    29521 accattatga aggtacttgt ccttatcata aagattgttt agaaggatta gcagcaggac
    29581 cgtctattga ggcacgcaca gggatcaaag ggcaagatct gccgcaagat catcctgttt
    29641 gggctattca ggcttattat attgctcaag cactgatcaa ctatacgttg acgttagcac
    29701 ctgaaaagat tattttaggc ggtggggtaa tgaatcagga tcatctcctt caaaaaatcc
    29761 gtcagcaatt tgttgaattg atgggtggtt atatggaaac acctcaagta agtgaatata
    29821 tcgttcgttg gggactgccg aatgaaagtg ggattatcgg tagtttgctg ttagcagaac
    29881 acgaacatca aaagtaagca gacaaattac acaaaaagaa cagagtctta tcattatctg
    29941 ctgataagac tctgttcttt tttatttcta ttcccataat ccaggtaaag ctttaccgac
    30001 tattttttct tgagtaaatg gatggatcat gtgcagttct aatgcatgga gcatcagtcg
    30061 ttcagctgtt ctattttggt agagagggtc tccaacgatc ggatgtccct tatgagctaa
    30121 atgaactctg atctggtgtg tacgtccggt ttccaaaaca caatagactt gtgtttgttc
    30181 agaaagtact ttgttgacag tgacatgtgt caaggcgctc tgaccatttt tatcatctac
    30241 gacccttttt ctccgatcat ggcgatctcg tccaatttta ttggtgatgg tcatatcttt
    30301 ggcaatttct ccccatacga tggcttgata tctccgatag atcatttttt gttccaacat
    30361 tcttccaagt atcggcaaga caaatggatt ttttgcaaat aaaacggcac cgcttgtttc
    30421 tttgtccaag cggtggacaa cgtaaggtcg ttgctgttta gtgtcgagat aagcagctaa
    30481 atgattgagt aaggtactgt tttctgtcgg ttcatttgga tgagtcttca ttcctgcagg
    30541 cttatccact ataatcaaat gctcatcttc atataaaaca cggatgtgct tcgcatctcc
    30601 taattgaact tcttgataat tataatctgt ttcttcgaat aacaatgtga tctggtcatt
    30661 gccctttact tcatgatgga acaaagcagg ttgttgattg atccaaacgt ttttacgtat
    30721 acgcaaaaag tgtcgaacct ttctaggaac taaccattcc ttttcaagca agtcgcgaat
    30781 tgttgtttca ggatggttca taggtaaagt gatcgtgatt ttcacaagtg tactcctttc
    30841 agtgcaattg cagactaatt gtaccatttt taaaacatat ttaagaaaaa acttacattt
    30901 ttctgttcat tcttatgtta ctctacttgc actaacgtaa aaaaacatat tgaactacaa
    30961 cttaagcagg aagaaaacac tctttcacta tggtaaaata agacgattga atcaaataga
    31021 caggagaaga agcaaatgga ttttcaaagc attttagaaa agataaaagc tgctttgatc
    31081 agtttttggc gttggatcag accatatctc ggacaattcc atcgatggcg aaagcgcatc
    31141 tggaaaaaat atcatgtgaa caaattgatt ttgctgattg gtttgatctg tgtcttagtg
    31201 acaagtatct acctctttat tttagcaaaa caagcaaatg tcgaaacgtt aaaatcagga
    31261 ttaagccaat cgacagtcat ttatgataag aaaaacgaag aagctggcac actgtacgcc
    31321 caaaaaggaa catatgtaga attaaacgcg atttctccct atatccaaga tgcagtaatc
    31381 tcgacagaag ataggaattt ttacgagcat cacgggtttg atatcaaagg gattgcccgt
    31441 gcagcagtcc gaatggtctt gtctggcgga acatcagggg gcggaggaag tacgatcact
    31501 cagcagcttg ccaaaaatgc gtatctgaca ttagaccaaa cgtttgatcg aaaagcgaag
    31561 gaattatttt tggcaatcga gatcgaaaag aagtacagca aagaagaaat cttgaccatg
    31621 tatcttaaca atgcctattt tggcaacggg gtatggggtg tccaagacgc ttccagaaag
    31681 tattttggag tagatgcaac gaatgttacg ctgtctgagg ctgctacttt ggcaggaatg
    31741 ctgaaaggtc ctggtattta taatccgatc gatcacccaa aaaatgcgaa tgatcggaaa
    31801 aacacagtac taagtgtaat ggaagaaaac ggaaaaatca caaaagaaca agaacaagca
    31861 gaaagccaga cagatatcag cgcgtatctt aatgacacat acgataattc agattctggt
    31921 tatcgttatc cttattattt tgatgcagtg atcgatgaag cagttaacga gtatggtctt
    31981 gatgaagaag atgtcatgaa taaaggctat aaaatctata cagctttaga tcaggattat
    32041 caaaagcaat tagaagctac ttatcaaaac agtgcattat tcccagcaaa tgcttcagat
    32101 ggtgccatgg tccaatctgc ttcagtagct ttagatccga atacaggtgg tgtacaagca
    32161 ctagtaggac gaagaggaga tcatgtcttt agaggattca gctttgctac acagatgcgt
    32221 cgttcaccag gatcaacgat caaaccatta agcgtatata caccagcact ggaagctgga
    32281 tacaagcctt ccagtatctt gaaagacgaa ccgcaaagtt attacgatgc gcataatttt
    32341 gacgggacat accaaggaga agtaccgatg tatcaagctg ttgcacaaag tttgaatttg
    32401 cctgctgtat ggcttttgca tgagatcggt ttacaaaaag gatatgataa agcagaagaa
    32461 ttcggcctgc ctttagcaaa atctgataag tattatggct tagcccttgg tggactggaa
    32521 aaaggagtat ctccattgat catggcgggt gcttacagtg catttgccaa tgatggtcag
    32581 atgtatacgc cgcatcttat cacgaaaatc gttgattcga ctggagcggt gatcgtcgat
    32641 aacacaaaca aaaaaccaaa acaggtcata tcaaaggaaa cagccgatga aatgaccagc
    32701 atgcttcttg gtacattttc aaatggaaca gcggctgctg cgaatcctgc aggttatacg
    32761 atagcaggga aaacaggaac cacagaaact aatttcgatg ccacaaaagc aaatgaccaa
    32821 tggatgatcg gttacacgcc agacgtagtg atttcgacat ggatgggtta cgaagaatcc
    32881 agcaagctgc actatttgga agggacaagt ggaaatgttg tcgggaaagt cttcaaatca
    32941 gcggcagaag gcatccttcc gtatacttca aaaacaggat ttagtgtagc agatgcctat
    33001 gccacaggag gacaagtggt agctgcagat caagttggaa acacaggaaa ttctaatgga
    33061 gagtctggaa attggcaaga taacttgaat caatatggtg aacaagcaaa agaagggctg
    33121 aaaaacttcg gcgatatggt gaaagaaggc gttcagggaa ttggtgaagc ggctaaagac
    33181 ctctggcgga aatatcaagg agagtagccc ctttgaaaac gaataacccc atgttacaat
    33241 agagagtaac tactaaaaga aaagagggca taacatgtca aacatctacg atagcgcgaa
    33301 tcaaatcgaa cgtgagatcc gtgaattacc agaattcaaa gcgttgaaag aagcgtacga
    33361 gaaagtacaa gcaaacgaag aagcacacaa actgttcaaa gacttccaag cgatgcaaat
    33421 cgaattacaa gaaaaacaaa tgagtggaca agagttcagc gacgaagatg caatgaaagc
    33481 acaagaaatg gctgtgaaaa tccaatcaga agaagtaatc aatgacttga tgcaaaaaga
    33541 gcaaggcttc agtacaatca tcaatgactt gaaccgtatc atcatgacac cagttcgcga
    33601 cctttacagc gaataaaact attttatgaa acaacagagg gtgtgaaaga gacgttcggc
    33661 cttgagcgat gacaaaattg cttcttattg ccgataggct gtctcttgaa caccatttac
    33721 caagaggtcg tgagcctacc gtttaactct gagttacaag gaacaatttc aaaaaacagc
    33781 ttctcatgtt tgatgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
    33841 attcagaaat aggagctacg acaaaagtct tgtcatagct cctatttttt gtcattcatt
    33901 tttcaagcgg tagaaatagc ctttttgagc taacagtttt tcgtaagtac ctatttcaac
    33961 gatttttcct tcatcgatca caacgatttt atctgcctct ttgatcgttg aaagccgatg
    34021 agccacaata aaagtagtgg gtgtttctgt caggtaattc agggcttgct ggattttttt
    34081 ctctgactgg ttatctaaag ctgaggttgc ttcatctagc aaaatgattt ttggctgacg
    34141 tatcaacgct cgtgcaatgg agatacattg cttttggcca ccagacagct tgttgccatg
    34201 ttcgcctact aaggtatcca gaccatcagg aagctggtca atcagtgaag aaagttgcgc
    34261 agcttcgatg acttcatcta aacgttcttt tgatacgttt ttcaagccat aagtgatgtt
    34321 ctctttgatc gaagcggaga acaacaaagt ggtttgagga acaacggcta aataatttcg
    34381 cacacttcgc atatccagtt catccaaagg ctgatcatct agaaaaattt ttcctgaagt
    34441 cggctggata aatccaatca ataagttaag gagtgtagat tttccagagc cagatggccc
    34501 aacaaaagca attgtttctc cagctttcac ctctagagaa aaatgatcga taatcggttt
    34561 gagtgagtcc ggataaagaa aagaaacatc ctcaaaacga aaatttccat ccaaatgttg
    34621 gatttttttc ttaccgtgat aagtttctac atcgccagca atcaacagtt cgccaataga
    34681 agtgatagat tccatgcctt ttgaaatcgt aggaatcagc gtgatcattc cagaaacgga
    34741 attgacgatc gtcgtgaaat acgtctgata catcacgaca tcgccgggtt ggatgcgccc
    34801 ttgtaaagct aatatcccag tgaaaatcaa acaaatcagc tgaaatatct gaaaactagc
    34861 ccagctgact gatccgaaaa cagattgcag gatatctaag tggaatcctt tttcatagat
    34921 ttgagtaaaa tgcttattta agcgagttgt ttcgtgtttt tctaacccat gagctcgagc
    34981 aatcggtgct aattcgatca cttcgatcac ttttgcagaa gtctcttcca tttcttgcct
    35041 gaagtctgag ttggactgtc tgatcctttt acggaaaaag agagtaacga atgcggcgag
    35101 aggaacagtc aataagaaaa acatgaaaac aacaggactt ttataaagcg tgatacctaa
    35161 agctacgaat aaattgacag cgatatttaa tccgctgaca aatacttgag aggacaacgt
    35221 ttggatagct tctacatcac ggatgatctt tgactggatt cgtccagatt gcaattcttt
    35281 ttgataagga atagatagtt cttggatttt cttgataagg gtcgctctta gtccagcttc
    35341 tacttgacgg atcgaagtgc tatggtattt tacatgaaaa taattcgtca atatattttg
    35401 accaatcaga caagccatca aaaaagcgtt gaaataaatg gcctgcaact gagaagaatc
    35461 tttcgatgaa gctgcattga ttacattggc aatagcgatc ggcattaacc aagatggaag
    35521 atgtttgata ataaaaaaga gtcctgacat ggcaaaacgc caataatttc cctgataaag
    35581 atgcagcaat gtctgaaaag catttgcatg atatttttga taacttttct gtattcgttc
    35641 ctccataatc ttctccttaa caatcttggt aaataaactg caagtgttct attgcttcag
    35701 gaacataaat ctcaggagta gcttcaagag tggcgtacat tagaggacgc tcatatttga
    35761 tataatgcag taccgggcgg aaatctaaac agcccttgcc aacaggtacc atgcggatct
    35821 ttccattttc catgatatag tctttgagat gaagcgctgt aatataacca tgtaaattgt
    35881 ccaaggcacc tcggatcact ttttcctgat cagcatgatt ttctttatgc atcaaattcg
    35941 cacaatctaa aatgattttt aaatttggtg aagctacttc atcgattaaa cgtttagcta
    36001 actgatagtt atagattgga tgattgatcc ctgcttcaac agcaacagtt acgccaaaat
    36061 tttctgcatg agcgactaat tcaatgatag aatttctggc gatctgataa gcttcttcag
    36121 taaaattttc ttttgtgtaa ccgacagaga cactaccggt ttccgtagca atcatcttgg
    36181 cttgataggc agagcacaga gcaatttgct gtttgaaagt tgccaatgct tgtgcacgaa
    36241 cttcttgatt tttgctggat aggttgatat agcatcctaa aatggaaagt tcgatattgt
    36301 ttttttggaa gtattcacca aaataggcag cactcccaag tgtcatgtca gttaatttaa
    36361 gttgttcagg aaatgctttt tgtggtgcaa attggatatg ggacaagtga agctgtgata
    36421 ctttttgaac gatttcttca cgatttcgaa catctaaatc gtgggcacgg atgcctaaat
    36481 ttaatggaaa catctcaaac tcctttttgt aaataattag caagaggcag acgggattct
    36541 tttatcaatt caacaattat tcgggcaact ttatcagcgc cttctggact aaagtgggta
    36601 ttgtctttta gtccttctgg gtaattttta caagtatcag gcataagatg caggtagaat
    36661 ttttttgtct cttcttttga gaaggtctgg aacaattctt gtgatcttga gaacacatct
    36721 aaaagaggaa tatggtgttt ctcagcaaat gcacgcatcg cttttggata gtctccaagt
    36781 atatctggat tcaatgttcc gttttctaag taatctcgtc gcgtcacaga tgtcagtaag
    36841 accatttgga cgttcgcttt ttttgcgcat tcaaaaaata tctgcagatt ttttaaatag
    36901 ctaccgaagg gaagcgtacc tctttcttct tggattttct ggtcgttatg tccaaattgt
    36961 acaaataaga aatcattcgg ctgaaactgt gaagctagac gggctaacct tccttcctgg
    37021 ataaatgatt tggtgctgcg tccattcttt gcttggttat tgatctggat ctcgggtgaa
    37081 aaataagcag agattttttc tccccaacct gtctcgggac gcttgttttc atctttttct
    37141 gccgcagtag aatcaccggc aatatggata gtaggcatgt tatctcttct ttcataaagg
    37201 tttataggca atgaagcaga acagcaagag aagaagacgg agcaa
//
LOCUS       DAFEPQ010000021        35736 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.021, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000021
VERSION     DAFEPQ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.021"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            144..542
                     /locus_tag="LP543_001160"
     CDS             144..542
                     /locus_tag="LP543_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBL2321337.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1221
                     /locus_tag="LP543_001161"
     CDS             526..1221
                     /locus_tag="LP543_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBL2321338.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1805..2692)
                     /locus_tag="LP543_001162"
     CDS             complement(1805..2692)
                     /locus_tag="LP543_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBL2321339.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2877..3716
                     /locus_tag="LP543_001163"
     CDS             2877..3716
                     /locus_tag="LP543_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL2321340.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3912..4886
                     /gene="sgrA"
                     /locus_tag="LP543_001164"
     CDS             3912..4886
                     /gene="sgrA"
                     /locus_tag="LP543_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBL2321341.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5184..5540
                     /locus_tag="LP543_001165"
     CDS             5184..5540
                     /locus_tag="LP543_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321342.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5543..6700
                     /locus_tag="LP543_001166"
     CDS             5543..6700
                     /locus_tag="LP543_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2321343.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6726..7166)
                     /locus_tag="LP543_001167"
     CDS             complement(6726..7166)
                     /locus_tag="LP543_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBL2321344.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7326..7832
                     /locus_tag="LP543_001168"
     CDS             7326..7832
                     /locus_tag="LP543_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL2321345.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            8000..9913
                     /locus_tag="LP543_001169"
     CDS             8000..9913
                     /locus_tag="LP543_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBL2321346.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9918..10919
                     /locus_tag="LP543_001170"
     CDS             9918..10919
                     /locus_tag="LP543_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL2321347.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(11121..11327)
                     /locus_tag="LP543_001171"
     CDS             complement(11121..11327)
                     /locus_tag="LP543_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBL2321348.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11973..12821)
                     /locus_tag="LP543_001172"
     CDS             complement(11973..12821)
                     /locus_tag="LP543_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL2321349.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12820..13887
                     /gene="gnd"
                     /locus_tag="LP543_001173"
     CDS             12820..13887
                     /gene="gnd"
                     /locus_tag="LP543_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBL2321350.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            13889..14890
                     /locus_tag="LP543_001174"
     CDS             13889..14890
                     /locus_tag="LP543_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBL2321351.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14887..15171
                     /locus_tag="LP543_001175"
     CDS             14887..15171
                     /locus_tag="LP543_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBL2321352.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15204..16001
                     /locus_tag="LP543_001176"
     CDS             15204..16001
                     /locus_tag="LP543_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL2321353.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            16015..16848
                     /locus_tag="LP543_001177"
     CDS             16015..16848
                     /locus_tag="LP543_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2321354.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16866..17723
                     /locus_tag="LP543_001178"
     CDS             16866..17723
                     /locus_tag="LP543_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL2321355.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17745..18254
                     /locus_tag="LP543_001179"
     CDS             17745..18254
                     /locus_tag="LP543_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2321356.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18291..18692
                     /locus_tag="LP543_001180"
     CDS             18291..18692
                     /locus_tag="LP543_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321357.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18804..19157
                     /locus_tag="LP543_001181"
     CDS             18804..19157
                     /locus_tag="LP543_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321358.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19191..19634
                     /locus_tag="LP543_001182"
     CDS             19191..19634
                     /locus_tag="LP543_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBL2321359.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19651..20736
                     /gene="uxuA"
                     /locus_tag="LP543_001183"
     CDS             19651..20736
                     /gene="uxuA"
                     /locus_tag="LP543_001183"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBL2321360.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20729..21442
                     /locus_tag="LP543_001184"
     CDS             20729..21442
                     /locus_tag="LP543_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL2321361.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            21771..23072
                     /locus_tag="LP543_001185"
     CDS             21771..23072
                     /locus_tag="LP543_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL2321362.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(23150..23299)
                     /locus_tag="LP543_001186"
     CDS             complement(23150..23299)
                     /locus_tag="LP543_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321363.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            23695..23895
                     /locus_tag="LP543_001187"
     CDS             23695..23895
                     /locus_tag="LP543_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL2321364.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            24462..25025
                     /locus_tag="LP543_001188"
     CDS             24462..25025
                     /locus_tag="LP543_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321365.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(25086..25430)
                     /locus_tag="LP543_001189"
     CDS             complement(25086..25430)
                     /locus_tag="LP543_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321366.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(25527..25772)
                     /locus_tag="LP543_001190"
     CDS             complement(25527..25772)
                     /locus_tag="LP543_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2321367.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(25842..26096)
                     /locus_tag="LP543_001191"
     CDS             complement(25842..26096)
                     /locus_tag="LP543_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321368.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(26260..26583)
                     /locus_tag="LP543_001192"
     CDS             complement(26260..26583)
                     /locus_tag="LP543_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321369.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            26770..26843
                     /locus_tag="LP543_001193"
     tRNA            26770..26843
                     /locus_tag="LP543_001193"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26804..26806,aa:Ile,seq:cat)
     gene            complement(27228..27725)
                     /locus_tag="LP543_001194"
     CDS             complement(27228..27725)
                     /locus_tag="LP543_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL2321370.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(27765..28574)
                     /locus_tag="LP543_001195"
     CDS             complement(27765..28574)
                     /locus_tag="LP543_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL2321371.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            28908..29765
                     /locus_tag="LP543_001196"
     CDS             28908..29765
                     /locus_tag="LP543_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBL2321372.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            29758..30660
                     /locus_tag="LP543_001197"
     CDS             29758..30660
                     /locus_tag="LP543_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBL2321373.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            30693..31043
                     /locus_tag="LP543_001198"
     CDS             30693..31043
                     /locus_tag="LP543_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL2321374.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            31092..32015
                     /locus_tag="LP543_001199"
     CDS             31092..32015
                     /locus_tag="LP543_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL2321375.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            32093..32986
                     /locus_tag="LP543_001200"
     CDS             32093..32986
                     /locus_tag="LP543_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL2321376.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            33024..33587
                     /gene="amaP"
                     /locus_tag="LP543_001201"
     CDS             33024..33587
                     /gene="amaP"
                     /locus_tag="LP543_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBL2321377.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            33601..33789
                     /locus_tag="LP543_001202"
     CDS             33601..33789
                     /locus_tag="LP543_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBL2321378.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            33802..34704
                     /locus_tag="LP543_001203"
     CDS             33802..34704
                     /locus_tag="LP543_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL2321379.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            34736..34978
                     /locus_tag="LP543_001204"
     CDS             34736..34978
                     /locus_tag="LP543_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL2321380.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(35234..35605)
                     /locus_tag="LP543_001205"
     CDS             complement(35234..35605)
                     /locus_tag="LP543_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321381.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT        12407 a         5739 c         7148 g        10442 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcatgcgata
     1081 acgttaggta aagagctact agatcaagta ttagagatgc cctcgattga taaagcagtg
     1141 aggggcttcc aagaagattg ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa
     1201 aaggtcgctg attacttctg aatcaatttc tccataaaat aaacgatagc cttctcgaaa
     1261 actcgaggag gctatctgac gattcaatat tcattaattt aaagtttggg tgactattac
     1321 ttatttctca tttaaacgat gtattcccta acctctaata catccttgta actctgaagt
     1381 tacgccagtt ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata aaaatatctt
     1441 tcgtgtttct ctagtataac agaataagag gaacaagtcc agaagggata tttttagtct
     1501 tttttaaaat aacttttcag tcgagtttga gtttatgata gttgtttttg aagcgatacg
     1561 gcaagtgaaa aaatagggaa gcatgaaaga atctacttaa tggcgcattg attcatgtga
     1621 tagaaataaa aaactttttt aaattatatc ctcatgattg taacaaatcc agtgatttgt
     1681 tttggtacaa ataaaaaaag gccccgatga agttgtggca aaagtggttt gcctcaaaaa
     1741 taagaaggaa tttccaaaaa tcacttctca aattttgcca catccccaat tgggtcaaat
     1801 ttgtttattt gaatatattt tttacataag gatctttaat agttacattg tcttttttca
     1861 tcactttacg gattgtttta tccatgaatt ccgtatcaag agtttttgct tcggtagcaa
     1921 ttttttctag ctcagatttg tatttgtctt tattagaacc tttatcctgt ttttttacca
     1981 tttttactag ataatagctt tgctgataag tcgttggatc tgttacagga atgatatcag
     2041 aaacttcccc gttttttagt ttaaatgctg ctttttttac ttcagtagga atctctggat
     2101 tggttgaatc aaaattcatt tttccatcag tttcctttaa tgttttattt tttccataag
     2161 cttgaacaag cttggagaaa ttttcccctt catttgcttc tttttttgct ttatctgctt
     2221 cttctttagt cgaaaataga atgatctgtg tactaacttc tggatagaat tctttccaag
     2281 cagtgtctag atctttatct gttaatttta cattggcttt caaaccgtat tggaaagcca
     2341 gcatctgttt aaagagtttc ttatatgtct cttctgtttg gccaactgct tttaattgtt
     2401 ccttgtagtt tggaccgagt tttttgattt gttcattata ttcttttgtt acttcctttg
     2461 aagaaatctc ttttccgtat acatcatcaa atgctttgaa gacaatcatt ttttttaata
     2521 gtgtttgatt atttgattta acttttgggt tatcataaaa atcctgaact gtaagtgttc
     2581 cacctttaaa cgttgcaatc gtttcattgg acttgctaca acctgagaat agtaaaattg
     2641 cacatgctga aaggcacata attagtagtt gttttttcaa aaaatttccc atcctttcct
     2701 tattaaaaac agtctaacag aagaattagg gagacagtct ttcattttcg taaggttgct
     2761 gtagatattc ttagttcgta aaaatctatt ctctctatga tggagaacat ttacatagtt
     2821 ttcacgagac aagaaagaaa agggttgtta aacttaatat aaaggaggga tgaaagatga
     2881 tcaggattat tcggttgatt ggatctgttt taatagtctt agttggtttt gttttaggaa
     2941 ttcttgtcaa taatgcactt gcggatatgt ttattggaaa tagtgaatgg attggagcaa
     3001 ttgccggtac ggtcggacat ttagctgttt ttttactagc tttatttctt gctagtaagg
     3061 tcgaaggaaa aagaatagaa gattatggtg tttctgttcg aggaaaagac tgggggtatt
     3121 tagcgggagg tctcgttgtt ggaattggag tgtttctgct tattactagt ccattatatt
     3181 tgacgggggc gtatcggtta gattcaggaa acgcaaacat tgtgccgctg ataacgagtt
     3241 ttattttatt tattgcagtg ggggcatcag aagagctttt attccgtggc ttttttcagc
     3301 atcagatgct tagatttggt ccgttaatag caatgattgg atctgctgca ttatttgctc
     3361 ttttgcacgg attgaatcct aacatgacat ttctggcagt tttcaatatc tttttagcag
     3421 gttgcttttt cagcgcactt atctacagta caggaagttt atttacggct attggtgctc
     3481 atattacttg gaattggaca caaggagcta ttttgggtat accagtcagc ggaacgcaag
     3541 aacaaggttt tttctccact ctgatccaat cgcaaaatac aataattact gggggaaact
     3601 ttggggctga agcatctata tcaactacat tattcttgtt agtcttgatt gttggtttgt
     3661 tactctatgc atataaaaca aagcggatga agaaatacac tgctgtttat caataaaaga
     3721 tgaagaaagc aaggctgata tcggtcttgc ttttttaagt ctgtcattga ttaaacgaaa
     3781 aagtaaacaa attcaagcga atacacgaca tcttttgtta gattttcaca actgactgtc
     3841 gtgttctcat cattttcatt ttatataatt cctttatcaa atagaaggta attatatgga
     3901 gagtgataga aatgaagaaa acagcgaccg tattatttgt tattcttggg ctgagtataa
     3961 ttgcagcaat gattggaatc agtacttctg caaatgaacg ggcaaatgag aatctttcat
     4021 ttactgtgaa aactgatcgg attgtttatg acatgacaca acaagtcatt actattccag
     4081 tcaaaccaaa caaaagcgtg aatgctagtg atgtacatgc tgttctaaca tatggttggg
     4141 atggtaatgg aagttcagaa aaagttattg gggaagttta tttaaaagac gtacaatgga
     4201 ccgctggtat tgagtataca ataatgattt ctgcagaact atcgattgat gagatcaaga
     4261 gtaaagataa agttgatttg attgtttttt atgatggaca gatgacgatc acggagaatt
     4321 tgaaaccaag cagttggaca gttgttggac cgccaagcag tacggatagc acaagtagtg
     4381 aaagcagtac agaaaacacg agcggtgaaa gcagtacaga gagtacgagc agtgaaagta
     4441 gcacagaaaa cacgagcagc gaaagcagta cggagagtac gagtagtgaa agtagcacag
     4501 gaagcacgag cagcgaaagc agtacggaga gtacgagtag tgaaagtagc acaggaagca
     4561 tgagtagtga gagtagcaca ggaagcacga gcagcgaaag cagcacagga aaagtaaata
     4621 atgaacttga tttggtacct atcataccaa ctaaggaaca aaagagaaat gcagggagca
     4681 acctaaaaag tgtgctaaaa cagcctgatt ttgtttctcg tggaattgat catcaagaaa
     4741 aagcgaaaga aacagataat agaaaaagcg atttaccaga aactggtagt agaaccttga
     4801 acaagctaat tacatggatg ggtgtgttac tcattttgat tgtaggagca agctattttc
     4861 gttctctctt ccatcgagtg aaataaagaa taaaagcaac agatctaaaa ttgaataaaa
     4921 cgatagattt ttgagtgaat atttcattcg agagtgctgt attgcctggc gaagttgtag
     4981 ctttgccaga tctatttttg tttactaaca tatttgagaa acaggaaata acaggaacac
     5041 aaatggaaaa taagagcaag aataattgac aatgcacact gcgcatggta gtatctctga
     5101 aaaatcagta acgaatctat ttgtacgtta ttcatgtagc tgtcggaata agtctaaata
     5161 atagataaaa aggagtatgt actttgtttg aagaatttat tgatatcaat gaaaggcaag
     5221 tatatcaatt tttgaactat tgttatgaaa gagacgagaa attatatgtg gtgaaggata
     5281 tcgctttaga tctaaactac accctggcta agatgaattc ggtgattcaa caagcagaat
     5341 cgttttgcga aaggtatcca gaatataaat tatcttttct ttcagaaaac aagatgataa
     5401 aagtcgagtt ttcttctcag tttttactga gtaaagttta ttctatactt ttagagggaa
     5461 cgattggtta tattttactg gattcacttt acaaaggtac ttatcaatcc ttagaaaatc
     5521 ttagtcaaaa aattatataa gtttgcgtac gattcaaaga aaattaagag aacttaatac
     5581 tattttgaaa aactatcatt tatcttgcag cttgaaaaaa ggtaatccga tagaaggaga
     5641 agaataccgt atccgatact tttttcactt gatgtattgg cagtcattcg acgaaaatca
     5701 ttctgatata ttatcaagta taaaaaataa acgtcaacta ttagaaaaat ttgcgaaaca
     5761 tgcgccatat acgcgtagta tagatagaaa gaaatggatc agcctactaa gaatttcgtt
     5821 gcaacgaatg aaacaaggcc actcgattac gtatgtccca gaagagatga acaaatttgt
     5881 gcatccattg ataacaaaag aagagtttac gcattacata ctagagcctt tttttgaagc
     5941 aaacttctgg tcgatgaatc acatcactcc aacagagcaa gcttaccttt attttatgtt
     6001 tggtgtaatg aacacctatt tggaagaaga tttgttggat actccagata aagtacaaga
     6061 aagttggggc tggattgaat cgtctattgt agaaaatttt gaaaaaaact tcaagatttg
     6121 ttttacaact tcagaaaaaa attatctata tgtcaatttg gcaatgatcc atttgtattc
     6181 aagggttttt gggacaaaaa agaaggtgga tgcttttgga aaatcagcag aagacagaga
     6241 ttttcagcat atatttccag tgatgtatcc aatcatgcaa cagtttttta gtggggtagc
     6301 tcgcagaatt ccagagctta atacttatta tgaaaagaac agacggttga tttttcaata
     6361 ctgtatgtta gtcagggata tttttataaa acacgaacaa cctgtcatat tctatattca
     6421 atcaaagtat ggtaaaacac aggaattatg ggcaaagaaa aggatattat ctatgactga
     6481 acgcccaata aaatatagtg catccgctga tcagttacct gatattgtca ttagcgatta
     6541 tcctatcgac aaaaaggcat atgatcaaac gaatacacag atattttact ggaatggaca
     6601 acctacaaaa aatgattggg cgcgtctgaa agctgaaatc ataaaaatca ggaatgagaa
     6661 aaatagatcc atattggaaa aattggattt cgttaattaa gaaaagcaga ttgtttatgc
     6721 caaaattaag acattgcaaa aagattattt tctttaaaat cgacaagatg accagaaagc
     6781 aagagttcgt tgagctcttg ctttctttta gttttagaaa ggaacatcct gattttatct
     6841 tcgctaaatc cagtcaaaag ttttgcagat tggtcgtaga taatcggttt tttcaataaa
     6901 gaaggggatg tacgaatctc aatatatagt tcactcaaac tgagttcatc tattctgtga
     6961 acaagatttt tataactaat agaatgagta gagagcattt cggtcacgcc gttgtttgtc
     7021 aattttagta agtggataaa ttcatttttg gttagtggtt gacgaattaa gttctgttcg
     7081 ataaacggta tatcatggac gataagccaa cgttttgcgc gtcttgaaga cgagcagcca
     7141 gaacctgtat acaatttaat catcatacat tcctccaata gctaagtatt tatgaaataa
     7201 taacatgaag aaatagtcga aaagtgccat ttagaggtgt aaaataagaa aatgttgtca
     7261 cgtataatgg aactagctag ataagaaata ctattaaatc aagaaaaaag aaagaaggca
     7321 caagtatgtc aaacacagcc gacgcaatag tagtgatcga tttacaaaaa ggggtatgtt
     7381 ttgattcttc gccgatacac gaattagaaa cagtgacagc attagtgaat aaaagaatca
     7441 aaacatacga aacgattggg aaaccagtta ttttcgttca acacgaagat gctcaattag
     7501 tgccacaaac aaaaccatgg gagctattgt cggaattatc tattcctgag cacagttatt
     7561 ttgtgagaaa aacccatgcg aattcatttt ataagactaa tttgagtgtt ttattaactg
     7621 aattggactg tcattcgatc gaattatgtg gggcacaagc agagtattgt atagatacga
     7681 ctgtgaagtt tgctcatgga ttaggatacc agcttatgat gtgtagagga gcaacgacaa
     7741 cttatgataa tccttttatg aaagcaaagg aaacaatcgc tttttacgag aaaatttggg
     7801 ataaacgatt tctgacattt ttggagcaat aagttgtttc ttcgtttaaa aaaagcagaa
     7861 tggatactgg tttatccatt ctgctttatt acgtaaaatg agaaaataat cctgttctat
     7921 ttttatggga taaaccattt attttttgta taaaaaatgt ttgcgtttac atataattct
     7981 cctagaaaca taaaaaggag tgaatagctt gagaacaaaa caagtattaa agggagcgtt
     8041 ggtcatctta tttgctgtaa gtacaagcgt cccgcaatgg acagtatatg gagagagcat
     8101 ttcttccgaa tcggaatctc gggaggtaga aaccacagaa actactgatg ctggaaatga
     8161 aacgaaatcg agtacttcac aaacagaaac aaatactgaa acaacggttg agacatctgt
     8221 tactagcaca gatcaagaaa cgaccgctac cacagataac caacaaacag aagaagcaac
     8281 agctacaaaa atgtcaaacg acgaatcctc ttctgctaaa gtaagtgaca aagagaatgg
     8341 gacagaaaat gatgatatcg gtaaaggtga aaatttcaat ggtaaagaat atttgaatta
     8401 cttcggtaaa cagatgacag aaatacttaa tggaccaaca gaagacatca agtcaaaatt
     8461 aatagagtta ttagtcactg agcttgctgt taatgattat ggtgtagata aagagattat
     8521 caaacatctg attgctactt atctattgta cgatatcgct tttgcaaaaa acagtgtgtt
     8581 gatgtcgatg cttggaggta cagatgatgg gacaacgcct acagaaaaca caggcttcta
     8641 tccaaataca aaacaacttc atcaaactgt actaaacaaa caagatggca aaatgatgga
     8701 tgtatatgca tattatgtcg atcaaggttc agataaaaca gtcatgatcc atggcggatt
     8761 tcgagggaat tggaataacg ggatcgttac agaagaatac aatgattttt ataaggccgg
     8821 gtataattta ttatttgttg attccagagc gactggtaat agcggaggcg attatgtgac
     8881 ctatggacaa tatgagtcag atgacgtcct ttactggata aatcaagaag ttcgagagcg
     8941 gccatctcaa aagattttac tttatggtgg ttcgatggga gcagctacga tgatgtctgt
     9001 attagcgaaa gatattcctg tcaatgtcaa aggaattatt gaaaattgcg gatttgcatc
     9061 aattgatgaa caactacgtt ttacgtacag ccagacagta gcccccgcac taccggatgc
     9121 cataaaaaat cagttagaca tcattgggga tcaagagcat gaagatttat ttatgggcct
     9181 tttgaaacaa tactattttg atcaggagat gcatttggat acaaaagctg ctctacctac
     9241 gattggaatg tcagattcat tgcctaaatt gattatccat ggaacagcag atgatgtagt
     9301 acctgtgtct aacgcgcaaa aattatatga gttatcagga gggtataaag atcttttatt
     9361 agtagaagga gcagggcatg gaaaggcaca ggaagttgac catgctgcct acacgaaaca
     9421 tgtaacggac tttttgaaag tagtgttcca tgatcagata aacgtaaagt atgtggatga
     9481 aaacaataaa agtctactca aagaccagga cgaaatccag ctttacggag cttatggaga
     9541 aaattatatg acagagcaaa agacattcga aggatacgaa ttagcaaatg ttgaaggacc
     9601 gactgaaggg atttttaatg agacgatacc aactattatt ttctattaca aaaaaatccc
     9661 agtagctcca ccaaaaaagc aagatccgac agagaatgca gacaataagg gaaagaatga
     9721 acctcgagag gatcaatcaa gtgatgatac gcaaccgatt cagatgacag tgaaagtaca
     9781 aaaaaagaca gatgcaaaaa aaagaatgtt gccgaaaact ggagaaaaga aacattcgct
     9841 tctcatttat tcaggtggat ttttatctgt tagcacgatt atcacatgga tttggcgaag
     9901 aaagagaggg taaatgaatg aaatggttca agcgaatagt gattgcagta ccagttatca
     9961 tcgtagttat tttagtcact gtatttttga ttaatcaaat cacaccaaag ccgattagct
    10021 tgcttgttag aaaacaattt gacacaagtg aaaaagaaca agtatatgcc cgaccagatg
    10081 atttccagca aaaaatcgaa caaatcgata taaaaaaaga aatatcctat ccttcaaagt
    10141 acaaaaataa taatatggat atctacacac cgaaagtgga aaagaaaaaa ttaccgattc
    10201 ttttctggat gcatggaggc gcatatgtag gaggggataa aaatgattgt cgtgattatt
    10261 tggaatatct atgttcagac acgcagcaaa tcatcgtaaa tatagattat gaacgatcac
    10321 cggaagcaaa acatcctaca ccagttatcc aattgaacga agccattcaa gcagctaaaa
    10381 aagagataaa agatcaagca gattggtcga atatttcgat tggtggaaat tcggcaggtg
    10441 cacaaattag tggagaatat ttgttagcac ttcaaaatga agaaattaaa aaagctgatc
    10501 atcttgatcc gactttggag aacaagcaga ttacgaagtt tatttctttg tctggtctgt
    10561 tggatccttc tgcacttact caagtaagtg ataaaatctc tagctttctt tatgaaaaat
    10621 gtggatgggc ttactttgat aataaggact ttgaacaatc agagaaggtc aaaagtttag
    10681 ccttggtcag acatgcggat cgttggacgc aaaacacatt cttaacggat ggaaatacaa
    10741 atacgtttac gaaacaaatg gaagaagtaa cttctgtatt tgagaaagcg ggggtgaaag
    10801 tcacaaaagt agattatgcg gagaaaaatg ctgttttgaa ccatgaatat cagtttgatt
    10861 tcagcaataa acaagcgcaa gaaacatatc aaaagttagt aatgtttttc aaggagtaaa
    10921 gtcttatgat gcaaaagagg ttgtaatctg tcacttagct ccaagtcaca aggagcaatt
    10981 tcaataaaca agcttttcat ggtttatgaa tattgtaact tgtgactgag gagttagaga
    11041 tggcaagggg caccttttat tcgaaatttg ggaatatcac acgattattg tcacgatctc
    11101 ttttacgtaa attttataag tcagatttgt tgttttgatc gtttaaaaaa agcaacaaat
    11161 ccgaagcctg cggaggcaag aagggacaaa aagccgagtg gtaagcaaaa gaatgctggt
    11221 tccactaata ttccgttgtt attgatataa ggctgggaac aaagatgaat agcaaataaa
    11281 atcaatgcac tagcgaaaaa aagcaaagaa atatggatag ctttcataag cagcacctcc
    11341 ttttttataa ttgtatgata aatagattaa gaatctataa atgtgacaag tttgttagga
    11401 tgtgaataaa atgatcatct agcgaacttg aaacaaagat ggctaatgat aagcaattaa
    11461 tgattcagca atcagatagg tacaaagttt accatcatac gtctagacca ataatagaaa
    11521 atatgggaat ataatagaaa agatttagga tacccccaaa agaatttccc caaaaattcg
    11581 atgtatccta aactttttta ttttacataa caatcatatc aactgtgaaa taaaattaca
    11641 aatgataacc taaaatgagg ccaaaagcca gtgttatcaa ggaaatactt aattcaccaa
    11701 aatttttttt aattttttag aaaataagaa acaaaaataa taaaaaaaag aaaagataaa
    11761 ttttttacta ctgtatacta actttgttat cgcatctaac aagtaactcg aataatacca
    11821 atacaacctt ctccaaagat gcgatgacat acgttctaac ccaccgacta ttgatgttct
    11881 gtaccaccct tgcagaacca aaaaagccac taccaaatat ttggtagtgg ctttttttgt
    11941 tttatggatt tttcgctttt tctgttatgg tgctattctt tgatttcaga aatagtatgt
    12001 cgaactttag cgatagaacg acgtgattct ttttcgttgt aaaacatcaa aatcacatat
    12061 agagagtcca aaatgcttag ttgagaaata cgggaagcta aagcttcaga tcggaattct
    12121 gtttcttcag aaatagaaat aaaaacgaca tcgccaagtt tagcaaggga agaattagct
    12181 tggctagtaa taacgattgt ttttgctcca gtttttctta cggtttcagc aatatggatt
    12241 gtttcttttg attgccctgt gtgagaaatc aaaacagcac agtctttttc cgtcaataaa
    12301 gaagcttcca tcaattgaat atggtaatcc gtgctgtgag aagttgcgat aggggaacgc
    12361 aaaaatttat gataagcgtc agttgctaca atctctgaac cgccaacacc gaaaaaatag
    12421 acacgtttag attcattgat aatagtagcc gcttttttta gatccttttg ttgtaacaac
    12481 ttttttgtgt catttaaagt tgaaatacta gactcgaaca ccttttgcgc catagtgagc
    12541 tcagtatctg ttttctgaat attttcgtgg atagaaatag cagatagatc attttcttgg
    12601 atcaacatgg ccattttaaa atctttaaat ccattataac caagtttttt tgtaaattga
    12661 aaaaaagtag agtcagcaat gcccagttca cttgctaaat tgctaatagg actatgactt
    12721 actttgcttg gattttctaa aatataatcg gcgatagcct gttcttttgg gctgaagtcg
    12781 ttgtacattg ttttgatttg tagtcttatg gagacgctca tgagaattcc tcattttcat
    12841 aataagatta tcattttttc cgttctcatt aatgttaaag atacaaggag agtaggaaaa
    12901 aatcaaacaa aaaatatttt tgtaaaatct atttacaaaa aaattttttt gtataatgag
    12961 ggtataagaa atggaggcgc tatcatggaa attggtgtaa ttggtttggg aaaaatggga
    13021 ttgaatttag ctttaaactt aaaagagcac catcacaaag tacaaggact cgacatttca
    13081 agtacagctg ttagtcaggc aagagaaaat gacattcctg cttatcaaga gatctctgaa
    13141 tttctagcta cttttgagca gaaaaaaatc atctggttaa tgcttcctgc tggagagata
    13201 accgaaaaca tgttaagaaa actttttcca aaactcggac caggagacat tattattgaa
    13261 ggcggaaact cttattataa agattcaatc cgtcgagcag aagagtttca gaaaaatgaa
    13321 atcggttact tggattgtgg gacttctgga ggaatcgaag gagcacgaca tcaagcctgc
    13381 ttaatgattg gaggagataa agaaactttt ctatccgtgg aacaattatt caaagatgta
    13441 gcaattgaaa gaggatatct gtatgctggt gcatccggaa gcggacactt cttgaaaatg
    13501 atccataatg gaatcgaata tggaatgatg caagcaatcg gtgaaggatt ccaacttgta
    13561 caaaaaagtg agtatgcttt tgacttggaa caagtcgcac gtgtttggaa tcatggttcg
    13621 gtgattcgcg gctggctgat ggaaatcatg gaagatcagt taaacgaaca tcccaattta
    13681 agtaattatc ggggaatcgt ttctgcatca ggtgaagcaa aatggacggt tgaaacagcg
    13741 ctagaaatgg ctgttccagt tccagtaatt gcaactagtt tgttcatgag gaatctttca
    13801 caggaagaag atagtttttc agcaaaagta gtttcagcac taagaaatgg gtttggcgga
    13861 catgaaatag ttgaaaaggg tgagtaaaat gccagattat catatagcaa tcgttaattc
    13921 cagcagtttc ggaagagtct ttccacacca cttggaaaga ttaaagtcag ttggagacgt
    13981 tcatttcttt actgttgatc aagagattcc aggaaaagaa ctagcggagt tacttaaagg
    14041 attcaatatc attattgcaa gtgttacgcc attttttcga cgagattttt ttgaaaacaa
    14101 agatgaactg ctattgattt cccgacatgg tattggttac aacaatattg atttaaaagc
    14161 agcaaaagaa caccagacaa tcgtatctat catacctgct ttagttgaac gaaatgctgt
    14221 agcagaaaat aatataacta atttgttgtc catgcttcga cgcacgacac aagcacaagc
    14281 aagagtcaag gacgatcgat gggaagatcg ggcggagttc gtcggtagga cgctgtttaa
    14341 caaaacagtt ggtgtgattg gtgcaggaaa taccggaagc ggagtaatcg aaattttgcg
    14401 taacggtttt cgctgtgatg ttctagctta tgatccttac aaatcagatt tatacatcga
    14461 aagctttggt gcaaaaaaag tagaacttca agaattactg gaacaatcag atatcatttg
    14521 tttatgtgcg aatttgacag aagagaacta tcacatgatt tctgataaag aagtcgatca
    14581 gatgaaagaa aatgtttatc tatctaatag tgcaagaggc gctctagttg atgaagaagc
    14641 gatgattaat ggtctgaaaa gcggaaagat cgcaggatat gccacagatg ttttagaaga
    14701 agaaccggga cgtcaagatc atccgtattt agcgtttgaa caagtagtga ttacgccaca
    14761 tatatcggcc tacacaaggg aatgtttaga agcaatgggt gaaaaatgtg tcagagatgt
    14821 cgaacagatc agtcagcacc ttcttcctga acgttctgtc cagacaaaga gcaactatat
    14881 aaaaggatga tgaagaaaat ggtgaacggt ttaaaagtga aaacaggccc tcaattttat
    14941 ttatatgaag aaggtggtat ttcaaaagtt tctgatctct tgaaatcata cggcgcgaaa
    15001 agagtactgg tcacccacgg aacggtatca tgggaaaagg cgctacctaa attagtattt
    15061 ttgaatgacg aaacgattca atttttttat catcgttatt ctggagaatg tagttatgct
    15121 gaagccagac gaatagctac aatcatcaaa aaaatgaaat cgatttcgta gttggcgttg
    15181 gcggaggaaa actgaccgat ctagtgggtt atagtgccaa tcttgcaaga atcgatttcg
    15241 gagtcatccc tactttagct agtaattgtg ctccatggac acctcttgct gtcatgtata
    15301 aggagaatgg acaagcagaa gggaaaacag aacactttta cagacaagct gcttttttta
    15361 taacagatcc ccaattagtg attgattccc ctgttccata tttcattgcc gggttagcag
    15421 atacactagc aaaatggtat gaatctgagc tcatcttaga acaagatttt ttgcaagatg
    15481 aatcattttt gaagctagca caagatactg cgaagatctg caaagaagag attttgaatc
    15541 attctgctaa agcaatccaa gatatggcac aaagaaaact gacatcggag ttcaaacagc
    15601 tttcagagat tatctttgct gttgctggtt tagttggagg attaggagat aaatacgcaa
    15661 gaaatgtggc agcacatgct atgcatgacg ctatcagtaa atatattccg gaaagccatc
    15721 gttttctaca tggagaaaaa gtagcgtatg ggattttcta tcaactggca ctagaaggtc
    15781 gctgggaaaa aatagacgag ttatatcctt tgtatcggga attagaactg ccggtttctc
    15841 tagaacaaat gggattattt ccgaaagata aacagataat agataaaatg gctgaattta
    15901 ttgattcgaa agagaaagtg catttgattc ctatagaggt aacacagaaa tcactgatac
    15961 agtcaatgat ggaactggaa ggttatatca gaaagatctg aaggaggagg acacatggaa
    16021 acattaagtg tagtacaaag tttgttgatt gctttatggg tagccgcggt tatgtcccgc
    16081 tggttaggtg gaggagccac gttgacctta cgtttttctc ctttgatgac tggattagtc
    16141 gtcgggttga tcatggggga agttgcacaa gcgatgattg ttacagccgc tttgcaaatg
    16201 atttatatgg gggttttttc tccaggagga tcaatgccag ctgaaccatc tattgcagca
    16261 gctatcgcag taccagtcgc attattaggg gatctgtcgc cagaagcagc gattgccgtt
    16321 gcggtgccag tcggattgct cggaagttac ttgtatcagt ttcgtttttt catcaatact
    16381 tttttaggta aatatacaga caaagctgta gaagatttga atacaaaagc tatggcccga
    16441 tcaattattc tttatccaac ggttgcctca tttttattat tcgttccgct tgttttcatt
    16501 gctttatatt taggggcgcc attgatcgct gatgtaatca aagctttaga gggaacagtc
    16561 gttatccaca ttcttgaagt agtaggtgga gggttagcgg caatcggtat cgcgacaact
    16621 gtatacgtta tcggtcgaaa agattatctg gtctttttct tccttgctta ttttatgagt
    16681 gttgttttga aatctttaga tattacgatg gttacttatg ccatttttgg gattattatc
    16741 gctttgatct ttgtccagtc gcaaaaaggg aaacaagtac agcctgccac aagcaataac
    16801 aattcagcat cttttgatga cgaagatgac tatgatgacg gattttaatt taggaggaat
    16861 gggaaatggc tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag
    16921 atgtaacaaa agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac
    16981 gttacttggc gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag
    17041 atgaagactc tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa
    17101 gctggggtgg cggtgtgatt actggcctaa tgtcttctat ggaacagcaa agagcagaag
    17161 aagagtatga agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg
    17221 gattgatggg ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata
    17281 tttttatcgc aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc
    17341 cttttatctg ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt
    17401 tcactatggg ccgtaatgct acaggcctaa agcaaaacac tggtgttcag acagcgattg
    17461 aaatgctatc agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag
    17521 tagaatcttc attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg
    17581 atcaaatcgt gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata
    17641 cgaaaaaagg attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc
    17701 tagcaggaat cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt
    17761 tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa
    17821 gtggagtgaa ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag
    17881 atctttacaa aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga
    17941 aagctaaatt ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa
    18001 aaacagtcca aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata
    18061 ttggagggat tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt
    18121 taaataaatc agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata
    18181 tttattttca agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt
    18241 ttgatttgtc ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga
    18301 acgagctgtt tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa
    18361 aaaagagtaa acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag
    18421 atgtgacagt cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag
    18481 atatcgaaaa agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag
    18541 gaagttattt catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat
    18601 tgtccttcat tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc
    18661 ctcatcacgt attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga
    18721 gctacttttt cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc
    18781 gaaatacttc atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg
    18841 tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat
    18901 cgggtatgaa aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc
    18961 tacagttttc gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt
    19021 tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct
    19081 atttgaccaa aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta
    19141 ttgctctttt tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa
    19201 tcatgatcgt tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg
    19261 tcgggacatc gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat
    19321 tcggagagaa ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat
    19381 ttgttgatct aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga
    19441 ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag
    19501 aagcaatcaa tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca
    19561 aacaaggaaa agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg
    19621 aggacgattt ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa
    19681 aaaggggatt cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt
    19741 ggaactttgc tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg
    19801 aaagcatctg ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac
    19861 gatgcaatca aggcagggac agatgaacga gattactata ttgataacta tatacgaaca
    19921 atcaaaaact tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt
    19981 ggatgggcga aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac
    20041 gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag
    20101 tcaaagggat tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg
    20161 gaaaaactgt atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta
    20221 gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat
    20281 ccgccatggg aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa
    20341 attctaaatg cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg
    20401 gcaaatccgg aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt
    20461 gtccacttca gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc
    20521 tctacagaag gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc
    20581 gacggtccta tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga
    20641 tatggcctgt atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata
    20701 agtaaggaaa aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat
    20761 ggatggcctt ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca
    20821 aaagtatgat tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat
    20881 ggcgataaga gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg
    20941 agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa
    21001 aaaaggagca gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc
    21061 ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt
    21121 gtttgatcaa atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt
    21181 tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc
    21241 gattgcaggg atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca
    21301 tttgataggg ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata
    21361 tgaatccaat aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga
    21421 aagtaaaggg ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc
    21481 ctgcacgact gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt
    21541 atttgtagtg aacaatttag aacaaacaca aaacgcatct actgttatct accagtagat
    21601 gcgtttaaag agaagaacga gaatcgtcca tatgagggtt tccacggctc tataatttct
    21661 aggactgata gaaaatcatt ctatcggtcc cttttgagca tgaaaaaaca aaacatacag
    21721 aagctccgtt agaattgaat caccacaaca caatcaaagg agcgttctgt atgcctgatc
    21781 ttcatcatat caaactacta cttggaatca aagataaaaa cattttcatc acaaatgtgg
    21841 aatcgaagag cattaagaat atcaagagtc ttgttgtttc tgcgacgtta agtaaagaaa
    21901 ttcgtcgctg tcctttgtgt aaacagatga atcatgaagg catgatcgtt aagaacggaa
    21961 agaaaaaatc gctcattcaa cttaataaat gtgcgaacca actgacttat cttgctttag
    22021 cgaaacaacg ctaccactgt cgtggctgcc acacctattt tacggcgaat acctacattg
    22081 tcgatagaaa ctgctttatc gctaaacaag tgcgctataa aattttagaa gagctaactg
    22141 aaaaacaagc aatgacgaca attgctaagc attgcggcgt ttcttggtcg accgtttctc
    22201 ggacactcgc ttctctgatc cccatgacaa aagtaaaaag aaattggtta ccgcgttgtt
    22261 tattaatgga tgaatttcgt tcactgaaaa atcaagtcgg tccctactcc ttttcttgta
    22321 tggatggcga tactggaaaa ttactggata ttcttccttc acggaagaaa aaggctctcg
    22381 tttcttattt tatgcaattt gaacgacgcg cacgattgaa ggtaaaggtc atcgtgacgg
    22441 acatgaatgt ttcttatgcc tctctgataa aggaatgttt tccaaaggca aaactcgttg
    22501 tggatcgttt tcacattgtc aaacatctca ttcgtaaatt tgaggatatt cgcgtacgaa
    22561 tcatgaaaag ctttgatcga aacgatccaa cacaagccaa atattatcga caattaaagg
    22621 cgttgtctcg gcttctgatc aaacgacagg acaagcttgt ttatgacaaa tggaccaaat
    22681 ggcgcaattt tggttgggcg tatctcaccg aaagtgaagt ggtggatcgt ctgctttcga
    22741 tctctgacga attacgaatc gcctattttt actatcaaga aattcttcaa gcgtttcacg
    22801 acaaagaggc ggacactttc tttaaacttg taagaacgat gcccaattcc gttccaaaag
    22861 aactgcattc tataaaaaaa gcttttataa actatgaatc cggcattcgt ttagccctcg
    22921 aattgcctta ttcaaatgcc aaaatagaaa atctgcatac ccacatcaaa gcattgaaac
    22981 gagtcgctta tggctttcga agctttcgga aaatgaagac cagaattttc ttactaaata
    23041 atctgattac ctatgaatca aaaaacatct aaagtagaaa aggaattcta ccttagatgt
    23101 tcttatctca ctctatcagt cctatttgac aaagagccgt ttccactcct tatgcagatt
    23161 tttttaattc ttgcactgct tgttcgatcg taacacctgt cgctacatga ttggtatttt
    23221 ctttgtcaaa aacgacaaat agattcaaat ctgtatccaa ggttactcga tattgtatat
    23281 ctttgttata gaatgtcata gccattctcc tttctttaaa tacttgctga aaggcaacga
    23341 acgcgttgat aacgaaattc catttataag tataccacca attgaaaggt tttcatagtt
    23401 aagaaatttt aaagtcaaaa gttgcagata aaagcaaata aacaagaaaa caaaaagtga
    23461 atcaatcgcg gttattcaac tttttcttcg attcgaagac aatagaagac aatgaatttt
    23521 atatttcatt gcattatcct tgtgttatta gaaaaagcat gatatattaa atgagtaatc
    23581 tattttgaaa cgtaatttga gcgatatatc tacatagata aaactccttt tgcctatcaa
    23641 atatttattg caacgaaaaa acgtacattg atacgtatca ggaggaattt atttatgaat
    23701 aacggtacag taaaatggtt caacgcagac aaaggttttg gctttatcac tggtgaagat
    23761 ggaaatgatg tatttgcaca tttttcagct atccaaggtg acggcttcaa aactttagaa
    23821 gaaggccaag cagtgtctta cgatgttgaa gatggtcaac gtggccctca agcaacaaac
    23881 attgttaaag cttaataaaa attgactttt aaccgtcttt tcgaaagaaa agacggtttt
    23941 ttgtacgaaa aaattcattg gtcgttgaat gggctgaata gtagtgtata aaacaatttt
    24001 gtggagcgtg aagcttttat ggtagaggaa aatacgaata aagtgatgtt gataaaaaga
    24061 ttcctatctt gaatatgaat cgagatgtct tgaaaatgaa tgagtttgac caatcattat
    24121 cctatcgaaa tttacagttt cgttcgattg gtctgtcaat taatgaaaaa gtaaaaaaga
    24181 atatgttcta ggaagagggc ggcacatcta aatttttaag taacggacag ttcaaatatc
    24241 aagcattgaa agaggctatc ctactgtttg aaggaatacg aatatataaa aacgacaaag
    24301 aggaaaaagt agttcattaa atcccttcaa gtgatgaaag aatgagatct aataaaacct
    24361 gatctttcat ctgttttttt aataacgtaa taattttttt attttttgtg ctttcgccgt
    24421 tattgtagag tgatttagca ttcttatttt taaaaataac tgtgttatat attaaaaaag
    24481 gcataatagt tataagcttt tttaaagaat atttagaaat tattaatttt cgtctgaaaa
    24541 caaaaaaaga aagcgttatt attttagtct attttcaggg ggggaacctt atgataaaaa
    24601 aactattttt actatcaata ttaacgctag caatggcagg ttgttctgac tctggcttag
    24661 caactgaact gatcacaaaa tcaactgaag ccacgacaga ggagttaaaa tgtggaaacg
    24721 atcacagtga ctacagaggc ttaaatcgac gatgcatggg aatattaggc agaagcaatt
    24781 gtaagcatcg atgtagtaat cgaagaaggt gtagaagaag caacagaaaa atcaaaagaa
    24841 agatcaaaag aagcaatctg tgctgtgcat cgtatacaga cgtcgaagat gcggtacttt
    24901 taaaccacgt caggagatgt tcatttatgt ctagggaagt tggcgatgaa ccaaaactag
    24961 agtacgcttt tagttttgca cgtgaggatg aagataaccc taaaacttac gacattttag
    25021 cttgatctaa gatggttaaa gctttttaat gaagtaagag gagatagaag gcttttctag
    25081 ttatattagg taagataaat tcctttttgc tccaaatttg ctaaacattc tgctagataa
    25141 acttcatcgt gttccggata aatcggcacg ttgcgctcta cagtaggtaa atcaaaaaat
    25201 gcttcgcatg atttgccacg atacatagga tttttccact ctggtgctgg gctgtaacca
    25261 cgtttttcca tttcatccat gacaagctga tgatattgga ataatctata aggagaatag
    25321 tgaaaaacat aatccacagt agcatgtttc tttccccagc catttcctct taatgcacag
    25381 cattcccgat gttgtcctaa aagctgttga cgaggaagct ttgaaatcaa tttttcatgc
    25441 cataatcaca tagaaatgtc tcctttatat atagattgtt aaccttagac gttaaatgaa
    25501 aaaaccagca ccaaggctgg aaaaaatcac ttcagtttgc ttgctgtttc tgagcgacca
    25561 attaggtaat caacggaaat atcgtaaaag tcagcgatat agattaattc aatgatgctt
    25621 ggaatttctt ctccttcttc gtacttacga tatttgaata aaggaatact taacgcgcta
    25681 gaaacagcta cttgagataa agatcggcca gttcttaaga aattcagacg acaacgtaaa
    25741 gtatccagta aaatagcttc ttcgatactc atttaatcac ctttcattat agtagaatag
    25801 ttataatgac tatagaaagc tacacatgca atcaatagta ctcaatgttt tttatttaaa
    25861 cgtatcaaat aaagaatgac gatcaataaa gacaaaacca gtcctgataa ttcttctata
    25921 aaaacactat cgggttcaaa gtagttgtaa ttaaaaactt tccacataaa gtagatagcc
    25981 ggaagagtca aaatagctaa ataaaaccgt tctttgttcc aattcataag ataaacaata
    26041 cttgcgacca gtgcgaccgc aggcacccaa aacaaaactc tcaccaacgt aatcatgtaa
    26101 cggactcctc aacgattcat ataataaaag ataacatgtt attgcataca tcacaaatga
    26161 aatgctagga aaataaaaga agaatagatc aatgaaaaat gaaaaagcag aacagttttc
    26221 tgctgataag caggaactgt tccgctgaat gtgtcttatc tatcgagaaa aaatcaatgg
    26281 attgatatct aagtaattca atacatataa aaagaggacg atcgcattca ctaaaaagga
    26341 aagaatgatg atcccatagc gaatggaaac atatttaaca aatacttttg tttgaatacg
    26401 ggcatatctt tttcctttat agctgagcat catgacaaga agcaaaaggg taatcacacc
    26461 ttgaaaaatc attccaataa acagaattcc tgaggtttga taatttaatt ggagaaaata
    26521 agctgctgcc gcaactaaat cgaataaaat cgcaataaat ggcaaaataa tcactcctaa
    26581 cattgagaat aaataaaaga ataacatttt ttctaaaata agcaaataaa aattaaagta
    26641 acaaaatagt tatgacacaa gtaaaagaaa taagaataac aatattattt tacttatatt
    26701 ttgaaacttg tgagagtttg tcattcgtgc tatagttgaa aagtaacaaa aataaatttt
    26761 atttatgatg tacctttagc tcagttggtt agagcagacg gctcataacc gtccggtcgt
    26821 aggttcgagt cctacaaggt acataagagg ttgtgaaaaa gctgccctgc atcaagtaat
    26881 aagaacagcc aagtaaaaac agcttcttat gtttttactt ggctgttctt attactgcag
    26941 atgcaagctt ttgaataccg tttaaggaag aggttgtgaa atcagcgttt tgacctgagt
    27001 attagcttaa caaacggaaa aatagttcct catattttgc cgtttgttaa gctaataccg
    27061 agggtcagct gattgaacac catttaagaa agaggtcgtg agcctgccgt ttcactctgt
    27121 gtcataaaaa agccaatcta ctttgataga aaaagcacgt aggactaaaa cagttctgcg
    27181 tgcttttttt attgtgtgtt aaatatcgta aatgaagata gatattttta tgattcgacg
    27241 atagagacgt tgaaatcttt caacagctct aaagcggatt ctccgtgttt tctactggag
    27301 actaacattg gatcaacaaa aaagtctgct tcaggaaaag cactctgcag gcctatcaga
    27361 ttagagagta cacacaagtt tgtttcaatc ccgactactt cgatctcttt ttgttcttct
    27421 ttgctttcaa ataaagtttc ctggatttca gctaaagttt caggtggaat cgtaaagtaa
    27481 tactttttaa tcactcgttc attttcttgt ggagaaagaa gagggatcaa ttgcaaatct
    27541 tctcgttctt cttctttgtc cttcagttca actggtatat cccgtgtgaa gagaatatat
    27601 tcattatttt ggcgtgcttt agctaatctt tgagcaatac gttctgccaa ttgatctgac
    27661 tctggaatat aaaattcgct gtttggatca agaatatgat tttgcatatc aataataact
    27721 aacattttct ttcctcctac attcatatcg tcattttatt ctttttaata gaagtttttt
    27781 aaaatataag caacaccagc ttcttcattc gtcagtgtta cttggtttgc aatttctttt
    27841 acttgtcgct gtgcattccc catcgcaata ctcaacccgc tgtaattcaa catatcaatg
    27901 tcgttttctt gatcgccaaa agccatgatg tcactcgatt cgattccaag gtaatccgcc
    27961 agaaaaacaa gtgaatgagc tttagtgata cctgtaggcg tgatttctaa gaaatgcgga
    28021 tcagagaata ctgattggat accatctgct tgcagatctc tttgtagctt ctgtagtttt
    28081 tcttcactct tatctgttaa tgctaattta agatagctaa gagaaggatc ttccaacata
    28141 tttaaaattt gatcaaaaga atggttctgt aaattgaaat gttcataata agggtcatct
    28201 ttgactagat ttgattcatc gaatgtcagc tgatcatctt gggtttctcg gctagcaatg
    28261 atggatattt tcgcttcttg agcatagaag aaggcttttc tcagtgtatc gctatctatt
    28321 tttgaacgtc tgagaacttt cgccattttt ccatccttca tttcagcaat cagtgaacca
    28381 ttggaagaaa tcaaatagcc accgattcct aattcggaaa aaatatcttt agcagaaatg
    28441 ctgttccttc cggtagcgac ggttacgatt ccgccatttt catcaaattt ccgaagtgct
    28501 tggatatttt cttctggtat ctttccattt cgatcaagca aggtaccgtc taaatcaatc
    28561 gctgctagtt tcattttatc ttcctacttt ctttttacat atctattttc ctgttacctt
    28621 aagattaaat gagaatttaa aaaaatgaaa ctaacttgct ttgtcattat gaattttttc
    28681 aaaaaacatt cttaagaaaa aggtggctat atatagtagt ttttggtcaa tacctttgaa
    28741 taaaaagcca tgaaaaacga atagttagcg cttaccgatg cgttttgtga taaaaaaatt
    28801 ttaaaatatt caatgaaaac aaagcatata ttttgcttta aaaccaaatg aggaatattc
    28861 tagatgtaga acaggttaga aaaagattag aaaaaggagt gattttgatg gagaagagat
    28921 acggcgtagt tcctgtggta actgcttcag acgaaaatta tgctccttac ttgagcgtga
    28981 tgatcgcaac tgcattagaa aattgcaata aaacaagacg cataaaattt tatgtgattg
    29041 acgatggtct atcagaatat agtaaagaag gattggaaga aacggtaaat aaatattcaa
    29101 gtaatgcaag tatccaattc ctgacagtgg aaaaagatat ctacgaagac ttcctagtca
    29161 gtgaccatat cacaactacc gcttacttaa gaatttcttt accaaactta ttagctaaag
    29221 aggattataa gaaagtcctt tacttagatt cagatgtgtt agtactagac gatatcgtaa
    29281 aactatatga tgagccgtta aatggaaaaa caatcggcgc tattatcgat ccaggccaag
    29341 tgaaagcttt ggaaagatta ggaatcgatt ccgatgactt gtacttcaat tcaggagtga
    29401 tggtgattga cattgatcaa tggaataaaa aagaaattac agaaaaaact atccattacc
    29461 tcagtgaaaa tggcgatcga atcatctatc acgaccaaga tgctttgaat gctgtacttt
    29521 atgaagattg ggaacaactt catccaaaat ggaacatgca gacttcgctg atttttgaac
    29581 gtcatccagc accaaatgaa aaatatgaac gtcagtataa agaaggaaat gaaaaacctt
    29641 cgatcgttca ctttacagga catgataaac cttggaatac gttgaaagat catccgtata
    29701 ccaatttata cttaaaaaaa ttagcacaca gtactctaac gaaagtaggc gagatcaatg
    29761 aataaaaaag aaatagcagt agtcgcaagt tgtaacacaa aattcgtacc acatttagct
    29821 gcattatttg tctcagtatt agataattgc aacccgtcta aatttgttcg gttctatgtg
    29881 attgacgatg atatcgattt tgaaagtaaa cagcttttac gcttctcagt gaaaaacgca
    29941 cgaatgaact cagatgtgga attcttgaaa atcaataaag aattctttac aaatgtggta
    30001 atcagcgatc gtattccaga aacagcttat tatcgtattg cgattccgga attgttccgc
    30061 ggcacagaag ttgaacgtat cttatatatg gattgcgaca tgatcgcttt acaagatatc
    30121 tctaaattat ggcgtcttga cttcggtgat tcaatcgtag cagctgttga agacgctggg
    30181 ttccatcaac gactggaaaa aatgaaaatt tcagctaaat caatgcgtta tttcaactca
    30241 ggtttgatgc tgatcaatgt gaaaaaatgg ttagatgaaa atattacgca aaaagtatta
    30301 gatttcatcg aacataatcc agaaaaatta cgattccatg accaagatgc attaaatgct
    30361 atcttacatg accgttggtt accacttcat ccgcgttgga atgcacaagg ctatatcatg
    30421 gcaaaagcaa aaaaacatcc aacagcagca ggtgaacgtg aatacgaaga aactcgcaac
    30481 aatccttaca ttatccattt ttcaggacat gtgaaaccgt ggagcaagga ttttgaaggg
    30541 ccaacgaaga aatattacga aaaatatgca gggatgacgg ctttccgttg tgtagctaaa
    30601 ttccctaaat atccaaaata tgcaaaagtt caaccaaaaa cagatgtagt tgttaaataa
    30661 tagaagacaa caactgaaga gggggagtag gaatggaaat tacaatgtct aaaaatgcag
    30721 tagagacgtt aattgaaaag gttggggaaa atacaaaaat cgcactcgcg cttatcaatg
    30781 attccgatcc atttctaagg gacaaaggtg cttttgcaaa aggcagcttt tttcaaatca
    30841 ttccttttgt ttcggaattt ggagaatatg cgactaaaat cgagcatcca ttattggaca
    30901 tctatacatc taaattagag caaaattatt tcggtaaacg attgaacatg gacttcaaca
    30961 aacagcttga cagcttttca ttagaaaatg aaatcgcagt actcgattat aatatcaaat
    31021 taaagaactg cttcttcagc taatagtttc gagaaagtcc aaaaaggagg tatacattga
    31081 gatactactt tgtggatgaa gaaaagaatg taatagaaac agatgaaaaa aaatataatt
    31141 ggctcgtact tgatagtgca aagcccgaag agattgagca agcaatcgaa gaattcgagc
    31201 ttcctgatga tattttcgta ggaaccgaat atccggaaga agtttctcgg atggagcgcc
    31261 tttatgagac gaaactagag catccatttt ctttggtagt gattaatcta gatcagtcag
    31321 ataatcgtat cgaaaaaaaa ttaacgccaa tatcttttgt actttccgat aatctcttga
    31381 ttacttgtat cgatagaaaa accgaatttg tggatcggct gctgacaaag catggaaatc
    31441 atttaaactc gtttgaaaag atcatcacgt atacattatt agatatttac acccattacg
    31501 tgaaagaact aagagagatg aaaaagcgaa ttgacgcttt ggataaagca gctcgtaaaa
    31561 caacggaaaa tgaagaactg tataaacagg cagatcttga aagagacatc gtatatatcg
    31621 atcatacctt aagagatcaa cgggaaacaa tggatcaatt atggaattct gaaactttca
    31681 tcaaacgttt agacgatgaa cagctactct atgatgtcca tttgagacag cgacaaacag
    31741 aaaaaatgat cgagatctat cgagatttat tagagagtat tggtgatcta ttcaacggga
    31801 tgatggacaa taatctaaat catatcatga aatatctaga ttcagctgca ttaatcattt
    31861 ccgttccggc gttgttcgca gggatttggg gaatgaatac aggaggatta ccgggtaaaa
    31921 gttctgctct aggctttata ctcgtagtct ttggctcttt gatagcagca gtagttttag
    31981 gttaccattt ataccggaaa gattatacaa attaaagaat ctatttaaaa caggtatcaa
    32041 acaaagaaat ttgttttggc aatacaattt aaaaactagg aggaatacag caatgacaga
    32101 accaaaatta atcgacccgc gaaacttgta ccatacggag aaatttccta aacaagatca
    32161 ggatacacca gctttacagc agaatatgat tcctgttcct gattgtggag aagaatccta
    32221 tgaaggcaat aatcaactag aaaaccgccg tgtactaatc actggtggag attcagggat
    32281 cggtcgtgca gcagcgattg catttgctag agaaggggca gatatcgctc ttcaattctt
    32341 cccaggagaa gaaaaagatg cagaggaagt agcaaaatac gtgaaagttg caggacgtaa
    32401 gattgtctta ctaccttttg atcttcgtga cgaacaagca cctaaagaaa tcatagataa
    32461 agccgtgagt gaacttggcg ggctagatac gatggtacta aacgcaggac agcaaatttc
    32521 ttgtcagtct attgctgagt taccacttga gcaagtcaaa gacacattta tgataaacat
    32581 catcgctatg tttgctttag tgaaagaagc tgttccgcat atcccagcag gaggagcaat
    32641 cgtaacaaca acttctgttc aagcattcaa tccaagcgaa catttattag attacgcagc
    32701 aacaaaagct tcaatcgcta actttacgat cggtttagca aaacagcttg cacctaaagg
    32761 aatccgtgta aatggtgtag cacctggtcc aatctggaca ccattacaac tagacgaagg
    32821 acaaccttct gattcgatcc ctgaatttgg tcagcaaagt ttactagaac gtgcaggtca
    32881 gccaggagaa ttagcaccag tttatgtttt cttagcatcg aataaagcaa gttatgttac
    32941 agcccaaatt tacggagtaa ctggtggaga agcgatcaac ttataagaag ttgatcgttt
    33001 cacccctatg aggaggtgcg aaaatgaata aaggtgtaaa aacgatcgga gtgatcggaa
    33061 cattactttt gctatctgtg cttttgtttg cgatactatt aagtgcaacc tatccattgc
    33121 cttttcagct agagaagttt cgattctttt ccatcactaa ttcatacgtg caacaatatg
    33181 tattttggct tgccgttgca tttgctattt tgacaattat cacgttactt gttttcctgt
    33241 tttatccaaa aacaatcgga aactttctac tgaaacatgg tgatggggag ttgacgttgg
    33301 ataaaaaagc aattgaagga atggttcgtt ctcacttgca tgaagatgaa ttcatccatt
    33361 caccaaaagt aaatgtcaaa gcaacgaaaa acaagatcgc cgtcaatgta aaaggggagt
    33421 tgaaacgaac ttcagcactc attggtaaaa caggaacatt gatgaaagaa atcgaaaatg
    33481 aagtacaaca catattggga acaaaagagc cagtgaaagt atcggtgaaa tacacaggct
    33541 ttgaagaacc agctaaacga agcaatgacc gcaaacgagt agaatagggg gggaccaaaa
    33601 atgaaagaac tattttcgac atacaagctc cctatcatct ttggggtact tggattgata
    33661 ttagccgttt tatggatcac cgtgggcttt ttcaagacct tattgatctt gatcatggca
    33721 gcgatcggcg tcgctatcgg tttttatctg aaacaaacga aaatcttaga tcactatttt
    33781 aaatcttagg aggaaacatc catgaataac aataacaaaa ataataacaa taaaatgaac
    33841 aaccaaaaac cagtgtctgc acatccagac ccaacgacac caaaccaatc aaatggtgtg
    33901 aacgatgaaa agaacaaaac agcaccagta tctgcacatc cagatccagt agtccctaac
    33961 gttcaacaag gagtagaaga gaagaaacat gatgcagaaa agcatgcacc tcatcatcca
    34021 ggaactccag aatctaaagt tccagaacaa catgatgcaa tcgatccttc taaacctgtg
    34081 gaagagcaaa aacatccttc tgaccatgcg aaagcacaag aaaaacttag ttctgaccac
    34141 acaaacggac aaggaaaact tggttctgat catacaaata accaaggaaa gcatgattct
    34201 gaacaggaag attcaatgcg cggtgagctg aattatgaag ataaagttgt gcaaaaaatc
    34261 atcggaatcg caattgaaca agtagacggt ttattatctg caaacggtgg tttcttctct
    34321 aatgtggcag gaaaacttgt gaatacagat aatgtaacgg ctgggatcga aacagaagtc
    34381 ggtaaaaaac aagtagcagt tgatatggat gtcattgtgg aatatggcaa agatatcgaa
    34441 aaaatctttg aagaaatgca agaagtaatc ggcaaagaag taaaaaatat gacacattta
    34501 gaagtgatcg aagtcaacgc taacgtagtg gacatcaaaa caaaagaaga atttgaaaaa
    34561 gatcaagaaa cggtgcaaga caaagtaact gacgcagcga aaaaaaccgg tgaatttgct
    34621 tcgaatcaaa cagataaagt aaaatcagca gctggaagcg gcgcacaaaa agtaaaagaa
    34681 aatacagaac cacgggttca gtaataagga agtaaaacat taggaggaac gaaacatggg
    34741 atttatttgg tcacttatcg tcggaggaat tatcggagca attgcaggag caatcacgaa
    34801 taaaggttct tcgatgggga ttattgcaaa tgtcgtagca ggtttagttg gatcagcact
    34861 tggtcaagca ttattaggtt cttggggacc aagtattgca ggaatggcaa tcgtgccatc
    34921 attgattggt gccatcattt tagtactaat cgtatcattc tttgctcgaa aactgtaaaa
    34981 agacttttaa aaagaggtcg tgaaaaagct gttcttcatc aagtaataag aacagccaaa
    35041 caaaaatagc ttctcatatt tttcatttgg ttgggcatat tatcgaagat gcaagctttt
    35101 gaacactgtt tatacggaat aagaggctgt gacaagactt ttgtcacagc ctcttattta
    35161 tgcgggcaaa ttcccaatta gatttttaaa agtttttcct taaatgtgtc tttattgata
    35221 cagagaagct tctttaataa aacaatgcct ccctaaattt tcttgagata ggaaaggtga
    35281 acatacaaaa taatacgacc actttgaaaa cccagatgat ccacaaatgg atcgtatgat
    35341 actgatgaag taaaaagcaa tggatcagaa actcaaagag attgaaaaag acaaggaaga
    35401 aataatatct cttttttact cttgtctgtt ttagccaata atagctcgta aagtaatcta
    35461 gacaaacaaa tagaagtaaa caaagtttcg taagagcagc aatcgtatca tgttgctctg
    35521 ctagacgatt gacgagcagg aaaaatgttg gtaatggaat atagatcacg agtaatgaaa
    35581 gaaaaaagta aacaataaat ggcatactat tcctcctaat tgtgtgagtt cgccctcatt
    35641 ttaaaataac gatatttgtt tgtgaagctt tttcgtgtcg taacaaatgt tatctaatgt
    35701 atttttaaat aaataatcat tattatcaaa ataatg
//
LOCUS       DAFEPQ010000022        33702 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.022, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000022
VERSION     DAFEPQ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.022"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            4..816
                     /locus_tag="LP543_001206"
     CDS             4..816
                     /locus_tag="LP543_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBL2321382.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="LP543_001207"
     CDS             813..1613
                     /locus_tag="LP543_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBL2321383.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="LP543_001208"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="LP543_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBL2321384.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="LP543_001209"
     CDS             3110..4819
                     /locus_tag="LP543_001209"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBL2321385.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="LP543_001210"
     CDS             5031..9383
                     /locus_tag="LP543_001210"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBL2321386.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="LP543_001211"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="LP543_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBL2321387.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="LP543_001212"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="LP543_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBL2321388.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="LP543_001213"
     CDS             11221..11514
                     /locus_tag="LP543_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBL2321389.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="LP543_001214"
     CDS             11511..11822
                     /locus_tag="LP543_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBL2321390.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="LP543_001215"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="LP543_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBL2321391.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="LP543_001216"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="LP543_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBL2321392.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="LP543_001217"
     CDS             complement(14623..14844)
                     /locus_tag="LP543_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2321393.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17654
                     /locus_tag="LP543_001218"
                     /pseudo
     CDS             15292..17654
                     /locus_tag="LP543_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18022..18945
                     /gene="truB"
                     /locus_tag="LP543_001219"
     CDS             18022..18945
                     /gene="truB"
                     /locus_tag="LP543_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBL2321394.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18949..19890
                     /gene="ribF"
                     /locus_tag="LP543_001220"
     CDS             18949..19890
                     /gene="ribF"
                     /locus_tag="LP543_001220"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBL2321395.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20238..21023
                     /locus_tag="LP543_001221"
     CDS             20238..21023
                     /locus_tag="LP543_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL2321396.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21036..21830
                     /locus_tag="LP543_001222"
     CDS             21036..21830
                     /locus_tag="LP543_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBL2321397.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKN"
     gene            complement(22010..22462)
                     /locus_tag="LP543_001223"
     CDS             complement(22010..22462)
                     /locus_tag="LP543_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2321398.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(22927..23295)
                     /locus_tag="LP543_001224"
     CDS             complement(22927..23295)
                     /locus_tag="LP543_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HBL2321399.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23445..23927
                     /locus_tag="LP543_001225"
     CDS             23445..23927
                     /locus_tag="LP543_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBL2321400.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            23964..24974
                     /locus_tag="LP543_001226"
     CDS             23964..24974
                     /locus_tag="LP543_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBL2321401.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25089..25802)
                     /locus_tag="LP543_001227"
     CDS             complement(25089..25802)
                     /locus_tag="LP543_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321402.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(25992..26582)
                     /locus_tag="LP543_001228"
     CDS             complement(25992..26582)
                     /locus_tag="LP543_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBL2321403.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            27066..27293
                     /locus_tag="LP543_001229"
     CDS             27066..27293
                     /locus_tag="LP543_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBL2321404.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27309..28475
                     /locus_tag="LP543_001230"
     CDS             27309..28475
                     /locus_tag="LP543_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBL2321405.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28709..29752
                     /gene="hrcA"
                     /locus_tag="LP543_001231"
     CDS             28709..29752
                     /gene="hrcA"
                     /locus_tag="LP543_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBL2321406.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            29846..30409
                     /gene="grpE"
                     /locus_tag="LP543_001232"
     CDS             29846..30409
                     /gene="grpE"
                     /locus_tag="LP543_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBL2321407.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30460..32289
                     /gene="dnaK"
                     /locus_tag="LP543_001233"
     CDS             30460..32289
                     /gene="dnaK"
                     /locus_tag="LP543_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBL2321408.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQGKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32440..33606
                     /gene="dnaJ"
                     /locus_tag="LP543_001234"
     CDS             32440..33606
                     /gene="dnaJ"
                     /locus_tag="LP543_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBL2321409.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT        11452 a         5812 c         7274 g         9164 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatcctg tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa acccgaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatattga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaaacgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga acctttcttt attgaaggaa atgattcaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa ttaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaatcgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac caggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt tttgcaagtt
    16741 acgaatcatt cttgcgagtg gttgactcta tgttgactca gcacttcaaa tggatgcgga
    16801 aatcacatga cctttcttgg cgaaatgatt atccttcgct aaatctcatt gcaagctcaa
    16861 cagtcttcca gcaagatcac aatggatata gccaccaaga tccaggaata ttgacacatt
    16921 tggcagaaaa gaaggcggag ttcatccgtg aatatctgcc tgcggatgca aatacattgc
    16981 tcgcagtcat ggataaagca ttccgctcgt cagaaaaaat caatttgatc atctcttcca
    17041 aacatccgcg tgctcaattt tactcagcag aagaagcagc cgtcttagtg aatgaaggct
    17101 tgaaaatcat tgattgggct tcgacagcaa aagaggagga acctgaattg gtcattgctg
    17161 ctgcaggaac ggaatctaac ttagaagcat tagcagctgt aactttgctg ctggaagaat
    17221 tcccaaaact aaaaattcgg ttcatcaatg tcgtagattt attgaaatta cgtcatccaa
    17281 gtcaagatcc gcgtgggttg agcgatgaag aatttgatca atattttaca aaagacaaac
    17341 cgatcttatt tgctttccac ggttatgaaa cattggtccg cacgattttc tttgatcgtc
    17401 ataaccatca tttaatgatt catggctaca aagaaaatgg agatatcacg acaccatttg
    17461 acatgcgtgt agtaaatgaa cttgatcgtt atcatttggc aaaagacgca gctttaaaaa
    17521 tcaaaggaag tcaagcagaa gattttgcga aaaaaatgga tcagaaatta caagaacatc
    17581 aaaactatat tcgtgaaaat ggtattgatc tgccagaagt attggattgg aaatggaaaa
    17641 atcttgacca ataaaatgat aaaatcgatc ccgcaaaata gaatttttta cattgattaa
    17701 gaaactgaaa aaataaataa aaaaaacgga aaaaagactg gaagaatggc cagtcttttt
    17761 tttcgttttt gggaagtgaa aaccaaacta tttacctgta agccacccat aaatccaatc
    17821 aaatggtgca aagaaaaaat cttccattct attccctcct ttaaaaaagc ataacatgat
    17881 ttctatttac gtgagcatga attttgttac gtttttattg ccgaaaaata aaaaccgttc
    17941 attatgatgt caaattcgtc agtaattgat gcaatgtgtt ataataattc agttaaatca
    18001 aaaatgaaat ggagaaaaga catggacggg ttattacctt tatggaaaga acgcggaatg
    18061 acaagtcatg attgcgtatt caaattaaga aaaatcttac atacgaaaaa aatcggacat
    18121 gggggtacgc ttgacccaga cgtagacggt gtgttgccca tttgtattgg aaaagccaca
    18181 aaagttatcg aatatttgac agattctggg aaaatctatg taggcgagat aactctaggt
    18241 ttcagtacaa cgacggaaga tcgatccgga gaacgggtag aaacaaaacc agtagatatg
    18301 tctttatctg aaaaaaaaat agatgaagtg atggcttcat ttattggtga gatcacccag
    18361 ataccaccaa tgtactcagc ggtaaaagtc aacggtaagc ggttatatga atatgcccgc
    18421 aataatgaaa cagtggagcg tcctgttaga aaggctcaga tttaccgttt tatccggaca
    18481 agtgaattga caagagacga agtgacagga accatttctt ggcgcttcga agtagaatgc
    18541 gggaaaggaa cctatgtccg cacattagct gtagatacag gcaagaaact tggttaccct
    18601 gcgcacatgt ctgatctgac ccgaacagca agtgccggta tgtcccagaa ggatgctctg
    18661 acattagaag aagtagctca agctgtgaaa aatgggacaa tcgatgattg tttgctgcca
    18721 atagaaaaag gagtggaacg gttcaaacga gtagatatta ctgatgaagt atggcagaaa
    18781 gtaaaaaacg gtatgagact tgattatact gtatttgcgc tcccagaaat gccgtcagaa
    18841 gaaattgctt tgttttataa aggaaaagtt gtcagtattt atcagccaaa cccgaaagaa
    18901 aaaaacaaat taaaacctag taaagtattg agaaatgagg aataacgaat gaatatcatc
    18961 aagatcagac atccttacca atcagaacaa attccagaag acgatgtcgt tttagtgctg
    19021 ggatttttcg acggcgtgca tatcggacac cagaaagtca taaagacagg tatggaaatt
    19081 gcacaaaaag aaggattgaa attagcattg atgactttca atcagcatcc atctattgtc
    19141 tttaaaaaga tcgatcctag cagcgtgaaa tatttgacta gccttaagca aaaagaagaa
    19201 aaaatggccg ctttgggtat agattattta tatgaagttg attttacttc ttcttttgct
    19261 catttagcac cgcaggaatt tgttgatcaa tacatcgtag ggttacatgc aaaatatgct
    19321 gtttccggtt ttgattatac gtatggacca aaagacattg cagatgtcgc tcattttcct
    19381 gaatatgcca aaggaagatt caaaatcgtc acagtttcta aagaagaaga acaaggtgaa
    19441 aaaatcagct ctacccgtat tcgtggatta ctagacagcg gacaagtaaa agaagcagca
    19501 aaactattag gttgcacata tgaggtagaa ggggtagttg tccatggcga tgcacgaggc
    19561 agactactag gattccctac agcaaatgtc aaagtaaaga gcacggttca tttgcctaaa
    19621 gaaggtgttt atgtgtcgga aataaaaatt ggggaaacat ggtatccagc catgggttca
    19681 attggacata atgatacatt cggtgaagga cgggagctga ctgtagagtt gtatattttg
    19741 gactttaatc aagatatcta tggtgaacat gtcgcggtac gctggaatca atttttaaga
    19801 gatcaagtga aattttctag tgtagaggaa ttgattgaac aactcaagga ggatgaacaa
    19861 gcaacagctg aatatttttc tcagcaataa aacattatgc ataaaatctc ttgtttatgt
    19921 ataactagca attacaataa aatgggtgat aaataagccc aggaaagggg ctgtgacaaa
    19981 agtcttgtca cagccttttt ttccaaataa atggtttttc agaagtagct tcttcggaaa
    20041 taagccgaaa tttcttgaag ctgaccactt ctgtcacaac ctctttcttt atagaaataa
    20101 aaggacacta gcaattcatg aaaacgattg ctagagcgta tcatttgctt tagactatat
    20161 atctgtacac tcactttaag gcggaaataa aagttatccg taataacagg tttaggtatg
    20221 aaaaaggagt gggtactatg tacaaggaat tagaaaacaa agtcgcagta gtaactggtg
    20281 gctcaaaggg gattggaaca gcgattgctc aacgattcgg gaaagaaaaa atgaatgtag
    20341 ttgtcaacta taactcggat aaagaaggag cagaaaaagc agtcaaagca atcgaagagg
    20401 caggcggaaa agcagtctct gtacaagcag atgtcagttc tgaagaagga atacagcgtt
    20461 tacttgatac ggctttagaa aattttggga cactggacgt ttgggttaat aatgctggga
    20521 tggaaaatca gcatccaacc catgaactta gccttgaaga ttgggaaaga gtgttaaaag
    20581 tcaatttgac tggcgttttt atcggaacga aagcggcttt aaattacttt acagagaata
    20641 ataaaaaagg aaatatcatc aatatgtctt ctgttcatga acagatccca tggccaacgt
    20701 ttgctcatta cgctgcttca aaaggcgggg taaaattatt taccgaaacg gtagcaatgg
    20761 agtatgcacc gaagggaatc agaatcaata gtataggccc tggtgctatc cgtacaccta
    20821 ttaatgcggc aaaatttgat gatcctgaac aaaaagaact cttggaaagt atgatcccac
    20881 taggacgcgt aggtgaacca aaagaaatag cggcgacagc tgcatggttg gcttcagacg
    20941 aatccagtta tgttacaggt attacactat ttgcagatgg tgggatgact ttatatcctt
    21001 ctttccaagg tggaaaagga taagggtgaa taaaaatggg tttattgatt gcattgatcc
    21061 cggcagttgg ctgggggatc cagccactga ttttgaaaaa gattggtggc agaccaacaa
    21121 atgagatatt aggaactggt attggagcgg tgcttgtcgg cttactcgtt caattattcg
    21181 cttctccagc tggtatttca gtgactacat ttttgattag tttactttct ggtgcatttt
    21241 gggttattgg acaaacagga cagtatacat ctttcaactt gattggtgtt tctaaaacga
    21301 tgccaatttc gactgcatta cagttagtcg gaacctcgtt gattggtgtc ttcgcttttg
    21361 gagaatggtc tggaagtatg gggaaaatca ttggagcagc agcaatcatt cttcttgtat
    21421 ttggttctgc tttaacggca atcagtgatg atcctggaaa aaaaggcggt atcacaaaag
    21481 gaattagtct attagcatca acaagtattg gttattgggt atacagtgca ttgcctaaac
    21541 tagttgatgc aagcggactt tccattttct ttccgcaaat gatcggtgtc tttttagggg
    21601 cagtgattta tgtgattttc cgtaaaccaa ccgcattttc tgaagacaag agttggaaag
    21661 caacgatcgt tggagccatc tttagtgtgt cagcattggc ttatattttt tcagcaaatg
    21721 aaaatggggt tgctactgca tatatcatta cacagctaaa cgtagttatc tctacattag
    21781 gcggtatgta catcctgcat gaaaataaga caccaaaaga aaaaaattaa cgattatcgg
    21841 attattgctg atcgtagtag gaagcatggt cacggtcttt atttgaggat aaagatggta
    21901 aaatttcatt tattaaaaaa atatttgttt gaaccaaaca gtaagtactt ataaaaaaat
    21961 agcgctagaa tttccaacat ctctggaatt tctagcgctt tttaattgtt taagatacaa
    22021 gggaaatatc tgtctggatc aataattttt cattgatcca ttgactaaag tttacgacat
    22081 aattgaagtt accaccgatt agtatctccg catgattgtc tttataaaga tcgtattgta
    22141 aaagacggtc ttttttattt gcgtaaacat cgttatggta ctggactaat tgttctcgga
    22201 attgattgat tgctgtaaaa tcacgatatt gaaggttgaa acgcatcgtg attggtgcat
    22261 ttagcatgaa tcgctgcatt tcttttgctg cttcctctgc ttgatctccg tataggaact
    22321 caaaaagatt acgttcagaa aatttccatt gcccgccaac ctttgttcct tttagttttc
    22381 cttcatgaag ataatttctg atagaacgtg tagtaacacc taattgttcg gctaattcat
    22441 ttactgagta aaaatctttc aatgttcacc ctcctatgtg aatggttcta ctgacttagt
    22501 tgatatttgc ttaatcacga aatgtagaaa gaacgatttt aattaaggtc attgtaaaaa
    22561 tcaagtcaga aaattctaac ttttatttct ctaaagaaat tcgttctctc ctaaagtgat
    22621 attatattta aatatacctg aaaataatgg gcagtattat gaaagatcac tgatattttc
    22681 tcgtattttt tccttattca aaagttttgc aggaaaattg ctgaaaaaat aattttacta
    22741 agtagaaaat tcaaagccat tcaatcagtg atttttttca gcacaagaaa gactccttat
    22801 aaaaatagca gatactttgt caattaagtg tttaaccagg gatgataaaa aggataggat
    22861 tgaacaaaac ctgtataagg tgatgaatag tttcctcgcc agtaaaaggt ttattcgtat
    22921 aaagctttat cgccaaaatt tatcatgacc ttttttatct tcaatattaa atgaaatcaa
    22981 ttgttcaatc aattcccagt tgattacact tttccaagga actttgatga cgttatccgt
    23041 atatacgtag cctgcttctt ggatattttt ttcaagggca cgaatcgctg ctttttccgg
    23101 tgcgatagaa aaatgatttt tagcaaaact aaatgcaata ataaaggtac catggtcagt
    23161 aaacataggt tggttccatt ttacagttgt gtctagctgg ggatacgttt ctgttatctt
    23221 agcaaaaagg tctttgatct tttgttgatg ttctttttct ttgatttgat tgatgatcgg
    23281 ctgaaatttt tccattctaa aacgcctcca ttaagtggtt tgatttattt taccagaaaa
    23341 acaatagtta tgtataaaaa acgaacaaaa cacaagtaat gttgtaaccg ctatatttat
    23401 atgataagat gtctcaaata ataaaataga aaagagacat aaaaatgatt gacttggcaa
    23461 agaaattctt acacgaaaat atatattttg agttgtatca tcatcgagcc atttatacaa
    23521 atgaagatgc tttggtaata aagaaagaac aaggatttac aggtacagag accaaaagct
    23581 tattcttaaa agataaaaaa gataataact acgtatatct gacttttact acgaaacaaa
    23641 cggatttcaa agcattaggg aagatcgtcg gtaaaaggct ttctattgtt tcatcagaac
    23701 gcatggaaga acaaacggga caaaagcagg gcgctgtttc tccgtttggt tatgaaaaac
    23761 agattccgat tattattgat gaagagctct tatcaaatga aaaacttgtt tttgcaccag
    23821 gtagaccaga tcaaacgatg gtcatccaaa cggttgactt aaacaaaatc atccagctac
    23881 ttgagatgcc gatatttttg atgccggcaa acgaaaaaaa caattaacca agtaaaatag
    23941 tgcatacttg gaggtgaagc aacatgataa gttatccatt attagaaaga aatgcaacga
    24001 ttggggttac tgcaccatcc tcaggcgtat cagaggaaca ccattcgtta ctcgaatcag
    24061 ctttaaagag aatggaagaa aaatctttcc aagtagttac tggaaagacg ctttggacgc
    24121 aagataagga aaaatcaggt ccagcaagaa aacgagcaga tgaattcaat caaatgatgc
    24181 aagatgaaaa tatccaaatg atctttccga cttggggagg agaattattg attgagattc
    24241 tcgattttgt cgattttgat gctctacaac caaaatggat agtaggttat tcagatatta
    24301 gtacgttgct acttgctgtc acattaaaaa cagggatagc cactgcacat ggaactaacc
    24361 ttattgattt aagaggagag tacttggatg aaacgacagc tatgtgggaa agtgtactga
    24421 aaacaagaga aggagaatcg attctacagc aatcttctga aaagtatcaa aaagtgtggc
    24481 cccatgagca ttcatcatct tctgttttcc atctcactga accaacaaat tggcaatcag
    24541 tcagcggaga tgcagtaagg atgaagggac gattattagg tggttgtatt gatacgatca
    24601 ggcatttaat cggtactcct tatggagatg tccaatcttt taaaagaaag tataatgatg
    24661 gaaagccgat cttgtggttt ctagaaaact gtgagctatc gtcagttgat ttaagaagat
    24721 cacttgttca gatgaaactg gcaggctggt ttgatggcag ctcggggatc atgttcggta
    24781 gaagtgcggc agaggatttg ttagattata aaatgaagga tgtatacgaa gatctgtcaa
    24841 aggaattaaa actaccgatc ttgtatgata ttgattgtgg tcatgtaccg ccacaactga
    24901 cattgattaa tggtgcctat gcagaagtag cggtagaaga tagaaaagcg actgttttgc
    24961 aaacgtttat ttaaaaaatt ttttcctgcc ttggagtcta tcaaagacca ataaatctaa
    25021 atagaaaaga aagtgaaaga agctgacaaa atgctgtcag cttctttttg tataacatat
    25081 tagaaagtct aattatctat aactgtcaca tcgattaact gtttcacgtc attgggattt
    25141 cctgtaggaa tagctactac agatgcatta tcaatgattt cttgagttgt ttctctaatg
    25201 aacgctggat aagatgtttg atctgcaggc atttgccaat gtgaaggaac tggataacta
    25261 gtgattgtcc cgtcatggtt acttgaatac actaaaactc cctggtaact gtatttccca
    25321 gtgagcataa ccacatcttt cggatagact gcactccctg catcataagg ggcgataggt
    25381 gttcctgcag gataatgaat aacatgtaat tcgaaatcac ccgatgtatc tctaaaagta
    25441 ttacctctaa tagctagcca aacacgtgcg tactcaattt gtagatcaga atatcctgct
    25501 aacggatcta ttgactgaga agactgtgag gaggttgtgt ctattgttga agaagaaata
    25561 tttgtctcaa ctgtactact agatgtagaa gaagtattgg gctctgagac tttactgctt
    25621 gttgaagatg tattcgtttc agtagaagct gatttctgag tacttgattt ttcagctgaa
    25681 ctggtttgtc tgattgaact tttgtctgtg gtcgcagatg gatcactatt ttctttacta
    25741 ggagacgtac atcctacaag gaataaagag accaacaata aaataaaacc taactttttc
    25801 atataaaata cctcattatt tttttaatac cagtatagca taatcctgaa tttttgaatc
    25861 cgtaaaacct tgtctatgaa tataaataga gtgtatcttc tttgataaat gtcagaaaaa
    25921 gtgtgacaaa taatcatcag taaaattact tgtcacacta ttgataagga gtatttaatt
    25981 gaagagaatg attaggagaa aggatctccg tactttttat agtaatcata gaatccaagt
    26041 ttgtatagac gaatcgtttt ttctcttatt tcttctgttt caaaatggac tgcttgaaga
    26101 atcaagtctg tgaattctat aggagctgtt ccattcgcag taatcaaatt acgatctttc
    26161 acagcttgtt gttctataaa atcttcttta ttctgataat tttccgattc taaccaagga
    26221 aattgcgcat ttccggtgtg tttgtaacct tgcaataatc cgctctgagc taaataatca
    26281 actgccccac aaatagcagc tactggtacg tctgttttta acaatttatc gattatttga
    26341 tataaatgat gattttttat gtcccatgaa ttgccaccga tcataataaa aaggtcaact
    26401 ttttcaggta tttgttcgat taagtaatcg atttttgttt ttaatcctcc aatggaagtg
    26461 acctctgatt ggatagaagc agttttgttt tgccatttct cattttggtt tagtgtgcta
    26521 gtaatataag caccttccca atcggcatat tcctctaata gaaacgtaat cgcagttttc
    26581 attttcaagc tctcctttac tatctataac aaggacaaca gttttaacct acagacattc
    26641 attaaaattg ggtagcgaca gcgaaaacaa caattcttaa gttttagaca gagtatacag
    26701 atagtcaact taattaaata cgaactaaat aaaaatgaca ggtaatcatt tttgtttacc
    26761 agcattataa cattttgcat acatggatga caaaagaacc aattcccact tgcttgagta
    26821 caatattaaa agaaactgga aggcaaaaag taattataga aacgaaaaat aaatcatatc
    26881 gggcactcag acatgaaaac aaccctagag atatataact aggtaaccag tttaatgaaa
    26941 gaaaaaatca tccaagaagt cgattcttgg attttttagt ggatttgttc gccaaatacg
    27001 tttagtatgc gtatatgaga ggttgtaagc aaagcgttgt ggtctgagca cttaaggagg
    27061 aatctgtgaa aatagctttt catatttttg cagattttga cttaatgccg aaggtcagct
    27121 ttgcgattac cgtttatcga gaggctgtaa aagcaccgtg tagctccgat gacaataagc
    27181 aagcattttt agagctaggt ttccttgatt ctaaatatgc ggcttatttc aatgagttgg
    27241 tgcttgaacg cgattttaag aaggtcgaaa acaggcacga cttttcgacg taaggagaaa
    27301 gaatttcaat gatagaaaca ataaatcccg cagaaaacaa ggtttctgcg tacattcata
    27361 tcccattttg cgagcatatc tgctattact gtgattttaa taaagttttt ttagaagggc
    27421 agccagtaga tgagtatgtg gaaatgctgc tgaatgaaat gaaaataatg ctagaaagtc
    27481 atccgacagc gaatttagaa acactttacg tagggggcgg gacaccaact tctttatctg
    27541 ctaaacaact tgatcgactg ttatcaggtg ctagagagat cctgcctttt caaaatggta
    27601 atgagtttac tgtagaagcg aatccaggtg atttgacccg tgaaaaactt caagtaatga
    27661 aaaactatgg cgtcaatcgt ttatccatgg gagtgcagac atttgataat cgcttattga
    27721 aaaaaattgg acgtaaacac accgcagaag atgtgtatca aactatgcaa tttttagaag
    27781 cagaaaattt taccaatgta agcatcgatt tgatttatgc attaccggga caaacactag
    27841 aaggatatct ggatactttg gaaaaagctt tggctttgga tctgcctcac tattctttgt
    27901 actcattgat cctcgaaaac aaaacaatgt tcatgaactg ggtacgacaa ggaagactcg
    27961 aactgcctga tcaggaaacg gaaaccagaa tgtttgaaga gacgattgaa gcaatggaaa
    28021 agcatggacg tcaccaatat gaaataagta actttgggct ctccggacat gaatctcaac
    28081 ataacctcat gtactggaac aatgaccact attttgggtt tggagctggt gctagcggtt
    28141 atcttggaga tattcgttac cgcaacaaag gaccaatcca acactattta cgcccattac
    28201 gagcaaatga acgcccggta ttggaagaag aaaaattgac acgaaaaaat caaatagaag
    28261 aagaaatgtt cttaggacta agaaaaaaaa cagggattac taaaaaacat ttctatgatc
    28321 gctatcaaaa aacaattgaa tcggtgtacg gtgatgtgct aagggaatta gagcaagaag
    28381 ggttacttgt taatgaagaa gaccgtgttt atttgacacc aagagggacc tttttgggga
    28441 acaatgtgtt tgagcgcttt ttgtttgaaa aatagtttaa agaatatttg ttttttatct
    28501 gaatttaaat aaaaaaagtt caaaaataca ataaaggact attgaaacgt tgatataact
    28561 ggatttacat caattattta gaattagcac tcttgtgttt agagtgctaa aaaatggagt
    28621 ctttctcttg acaggagaag ggcttcttgc tatatttata agtggattag cacttagaat
    28681 atatgagtgc taatgagagg tgaggaagat gttaacaaag cgacaaaacg acattttacg
    28741 tctgatcatc caacactata cgagtagtgg cgtgcccgtt ggttccaaaa cattgatgtc
    28801 tgagggagtc aaggccagct cagcaacgat tcgcaatgat atgaaagcat tagaagatga
    28861 aggattgctg ttgaagactc actcttcttc tgggcggatt ccatctgctt taggctatcg
    28921 ttattacgtg gatcatttgt taaaacctgc tcgcgtagca gaagatgatt tgctgctgat
    28981 tcgtcagtct ctaggcagag aatttcatga aatcaatgag attattaagc aatctgctga
    29041 gatattatct gagctgacaa gctatacgac tttttcattg ggacctgaaa taaaagaccg
    29101 ccgactgact ggctttcgta tcgtaccctt gaataatcga cagattattg ctatcgtcgt
    29161 aacagatatg gggaatgtag aaagccaagt gttcactctt cccgaatata tgggaagtca
    29221 ggaactagaa aaaatggtac agattgtaaa cgaccgtcta gtcggagatc ctctgatgac
    29281 ggtgtaccat aaacttcgta cggagattcc gatgattctt cataaatatt tccaaacgac
    29341 agaagggatg tctcagttat ttgacaccgt tcttggtcat gtttttgaag ataaggttta
    29401 tgtcagtgga caaatgaatc tcttggatta tgagccccgt caagatcttg atcagttcaa
    29461 atcgatgttt tcttttatga aagactcaga cggacttaca caaatgatca ctccgatgga
    29521 tagcaatatt catatcaaga ttggtgctga attaggcaat gatttattgc agaatatgag
    29581 tatgattcaa gcaagttacg agatagatgg tcatggaaga ggtacaatag ccttgttagg
    29641 gcctactagt atgccttatt ctcggatgtt cggtctagtc gatgtttatc gaagagaact
    29701 agcagctaaa cttgctgatt actaccgttc gcttgacttg tcaaactcgt aatgtgaata
    29761 acgaagctat ttgcagctga tcgttctctc tttcttagta agagcaatca aacagctgac
    29821 tgaataatga aaggaagact gtatcgtgaa agaaaatcaa gaagaactag ataaagaaat
    29881 gacagatgct cagccagaac cggaaatcga tgtcgaagct gcagaagatt cgggtatttc
    29941 agaagctgaa gcggaagaat tcgaaacagc gaaattaaaa gctgagttag aagaaatgga
    30001 agataaatac ttgcgggctc gtgctgaaat cgcaaatatg gcaaatcgag gaaaaaatga
    30061 gcgtgagcaa ttacaaaaat accgttcaca agatttagcc aaaaaattac ttccatcaat
    30121 tgacaacctg gaacgtgcat tagccacaga agtgtcagat gatcaagggg caggtttgaa
    30181 aaaaggcgtg gaaatggtct tggaaagttt gcgtaacgca ctggaagaag aaggaattga
    30241 aaaaatccct gctaaaggag aagcctttga tcctaatctt catcaagcag tacaaactgt
    30301 tccagcgact gaagatacac cagccgatac gattgtagaa gtactacaag aaggctacaa
    30361 attacatgat cgcgtgttaa gaccaaccat ggtcattgtt gcacaataat tgtgcggata
    30421 taaaacttac actttcaatt aaagaaggag acgttaatta tgagtaaaat tatcggtatt
    30481 gacttaggta cgactaactc tgcagtagca gtattggaag gaaacgaagc aaaaatcatc
    30541 gctaacccag aaggaaaccg tacaacacct tctgttgtat cattcaaaaa tggagaaatc
    30601 caagtagggg aagttgcaaa acgtcaagct gtgacaaacc caaatacgat ttcatcaatc
    30661 aaacgccata tcggtgaagc aggttacaaa gtagatgtag aaggtaaatc atatacacca
    30721 caagaaatct cagcaatgat ccttcaatat atcaaaggat tcgcagaaga ctatttaggt
    30781 gaaaaagtcg aaaaagcagt tatcactgtg ccagcttact ttaatgatgc acaacgtcaa
    30841 gcaacaaaag atgctggtaa gatcgctggt ctagaagtag aacgtatcgt caatgaacca
    30901 actgcagctg ctcttgccta tggtttagat aaaacagatc gcgacgaaaa aatcttagta
    30961 ttcgaccttg gtggcggtac ttttgacgta tctatccttg aactaggaga cggtgtcttt
    31021 gatgtattgt caacagctgg cgacaaccat ctaggtgggg acgactttga taacaaaatc
    31081 atcgactaca tggtagcaga attcaaaaaa gaaaacagca ttgacttgtc tcaaggcaaa
    31141 atggctttac aacgcttgaa agacgctgct gaaaaagcga aaaaagattt atctggcgta
    31201 tcaagcacac aaatcagctt gccatttatc acagcaggag aatctggacc attgcatttg
    31261 gaaatgacat tgacacgtgc aaaatttgat gaattaacag ctgacttagt agaacgtaca
    31321 aaagtaccag tacgtcaagc gctaaaagat gcaggtttat cacaatcaga aatcgacgaa
    31381 gttattttag tcggtggatc tactcgtatc cctgcagttg tagaagcagt acgtaaagaa
    31441 acaggaaaag aaccaaacaa atcagttaac cctgatgaag tagttgctat gggtgctgct
    31501 atccaaggtg gcgtgatcac tggtgatgtg aaagatgttg ttttacttga cgttacacca
    31561 ttgtcattag gtatcgaaac aatgggtgga gtgttcacta aattgattga ccgcaataca
    31621 acgattccaa caagtaaatc acaagtattc tcaactgcgg cagataatca accagcagta
    31681 gacatccatg tattacaagg tgaacgccca atggctgctg acaacaaaac tctaggaaga
    31741 ttccaattga cagatattcc agcagctcct cgtggtattc cacaaatcga agtaacattc
    31801 gatattgata aaaacgggat tgtaaatgta tctgcgaaag atctaggtac acaaaaagaa
    31861 caaaaaatca cgatcaaatc ttcttcaggt ctaacagacg aagaaatcga acgtatggta
    31921 aaagatgcag aagcaaatgc agaagcagat aaagctcgta aagaggaagt tgacctacgt
    31981 aatgatgtag atgcattgct attctctgtg gacaaaacat tgaaagaatt agaaggcaaa
    32041 gtggacgaag aagaagtgaa aaaagcagaa acagctcgcg atgaattgaa agccgctgtt
    32101 gaagcaaaca acatcgaaga aatgaaaact aaacgcgatg cattgaacga aatcgttcaa
    32161 aacttgactg ttaaactata cgaacaagcg gcacaacaac aagctcaaga aaacccagaa
    32221 gcagctcaag gcggagcaga tgatgtcgtt gatgctgatt ttgaagaagt cgatggcgac
    32281 gataaataat cagttcactg aatttcgaga gaaggccaaa gttgctactt tggctttttc
    32341 ttttgaaaaa tagtttgaag tatcggtttt tgtttcaaac aaaatatgat atgataacaa
    32401 gcgacgtgaa aatgatgaga accaatagga ggaaagccta tggcaacaaa acgtgattat
    32461 tatgaagtct tagggctctc aaaaggagca tcagaagatg aaataaagaa agcttatcgt
    32521 aagctttcga aaaaatacca tcctgacatc aataaagaac ctgatgcgga agaaaaattc
    32581 aaagaagtat cagaagcata tgagatttta agtgacccgc aaaaacgcgc tgcatatgac
    32641 caatacgggc atgcaggcac agatccaaat tacggtgctg gtggagctgg tggcggattt
    32701 ggtggattcg gcggcttctc tggtggcggc ttcggtggtt ttgaagatat cttcgactct
    32761 ttctttggag gaggcggtcg gtcagtcgat ccaaatgcgc ctcgtcaagg tgctgatttg
    32821 caatatacgg tcgacttgtc ttttgaagaa gcgatttttg gtgtagaaaa agaaatcaag
    32881 tataatcggg aagaaatctg tcatacttgt ggtgggaacg gtgcaaaacc aggcacacaa
    32941 ccaactactt gtcataaatg tcatggtgca ggtacgatta atgtagaacg ccaaacacca
    33001 cttggacgag tgatgagccg tcaaacttgt gatgtttgtc atgggacagg gaaagaaatc
    33061 aaagaacctt gtccaacctg tcatggctca ggacatgaga agaaagcaca ttcagttaaa
    33121 gtgaatgtac cagctggtgt agaagaagga caacaaatgc gtttagctgg tcaaggagaa
    33181 gctggagaaa acggcggacc atttggtgat ttgtatgtgg tcttccgtgt agaagaaagt
    33241 gatatctttg atcgtgaagg ttctgaaatt tactatgaat tgcctttaag ttttgtacaa
    33301 gcagctttgg gggatgaagt ccaagtacca actgtccatg gagatgtgaa attgaagatc
    33361 cctgctggaa cacagacagg tacgaacttc cgtttacgtg gaaaaggcgc tccgaagtta
    33421 cgtggtggtg caacaggcga ccaacatgtc aaagtaaaac tgatcacacc gaaaaatcta
    33481 aatgaacaac aaagagaagc attacgtgca ttcgctgaag ctggcggcat caaagttgaa
    33541 gaacagcaag aagacggttt cttcgataag tttaaagatg cttttggcgg gaaaaagcgg
    33601 aagtagtttt tctagtaaaa acgctaagtc attaataatt aaaaaccaga cgaacaatca
    33661 tatgttcgcc tggttttttt gtagttgtca tgtttgtacc aa
//
LOCUS       DAFEPQ010000023        31551 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.023, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000023
VERSION     DAFEPQ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.023"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(862..1203)
                     /locus_tag="LP543_001235"
     CDS             complement(862..1203)
                     /locus_tag="LP543_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL2321410.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1203..1433)
                     /locus_tag="LP543_001236"
     CDS             complement(1203..1433)
                     /locus_tag="LP543_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL2321411.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1720..2058
                     /locus_tag="LP543_001237"
     CDS             1720..2058
                     /locus_tag="LP543_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321412.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2263..2502)
                     /locus_tag="LP543_001238"
     CDS             complement(2263..2502)
                     /locus_tag="LP543_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBL2321413.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2478..2825)
                     /locus_tag="LP543_001239"
     CDS             complement(2478..2825)
                     /locus_tag="LP543_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBL2321414.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(3557..4138)
                     /locus_tag="LP543_001240"
     CDS             complement(3557..4138)
                     /locus_tag="LP543_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL2321415.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4321..4947)
                     /locus_tag="LP543_001241"
     CDS             complement(4321..4947)
                     /locus_tag="LP543_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL2321416.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4969..6300)
                     /locus_tag="LP543_001242"
     CDS             complement(4969..6300)
                     /locus_tag="LP543_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBL2321417.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6455..6856)
                     /locus_tag="LP543_001243"
     CDS             complement(6455..6856)
                     /locus_tag="LP543_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBL2321418.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6942..7148)
                     /locus_tag="LP543_001244"
     CDS             complement(6942..7148)
                     /locus_tag="LP543_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321419.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7243..8745)
                     /locus_tag="LP543_001245"
     CDS             complement(7243..8745)
                     /locus_tag="LP543_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBL2321420.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8763..9218)
                     /gene="argR"
                     /locus_tag="LP543_001246"
     CDS             complement(8763..9218)
                     /gene="argR"
                     /locus_tag="LP543_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBL2321421.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9316..9933)
                     /locus_tag="LP543_001247"
     CDS             complement(9316..9933)
                     /locus_tag="LP543_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBL2321422.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10154..11527
                     /locus_tag="LP543_001248"
     CDS             10154..11527
                     /locus_tag="LP543_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2321423.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11602..12714
                     /locus_tag="LP543_001249"
     CDS             11602..12714
                     /locus_tag="LP543_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBL2321424.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12786..14423)
                     /locus_tag="LP543_001250"
     CDS             complement(12786..14423)
                     /locus_tag="LP543_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBL2321425.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14591..15199)
                     /locus_tag="LP543_001251"
     CDS             complement(14591..15199)
                     /locus_tag="LP543_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL2321426.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(15583..16734)
                     /gene="queG"
                     /locus_tag="LP543_001252"
     CDS             complement(15583..16734)
                     /gene="queG"
                     /locus_tag="LP543_001252"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBL2321427.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(16753..17505)
                     /locus_tag="LP543_001253"
     CDS             complement(16753..17505)
                     /locus_tag="LP543_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321428.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(17646..18596)
                     /locus_tag="LP543_001254"
     CDS             complement(17646..18596)
                     /locus_tag="LP543_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBL2321429.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(18597..19436)
                     /locus_tag="LP543_001255"
     CDS             complement(18597..19436)
                     /locus_tag="LP543_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL2321430.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(19433..20179)
                     /locus_tag="LP543_001256"
     CDS             complement(19433..20179)
                     /locus_tag="LP543_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL2321431.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            20417..21787
                     /locus_tag="LP543_001257"
     CDS             20417..21787
                     /locus_tag="LP543_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2321432.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(21842..22738)
                     /locus_tag="LP543_001258"
     CDS             complement(21842..22738)
                     /locus_tag="LP543_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL2321433.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(22852..23022)
                     /locus_tag="LP543_001259"
     CDS             complement(22852..23022)
                     /locus_tag="LP543_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321434.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(23019..23696)
                     /locus_tag="LP543_001260"
     CDS             complement(23019..23696)
                     /locus_tag="LP543_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBL2321435.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(23787..24692)
                     /locus_tag="LP543_001261"
     CDS             complement(23787..24692)
                     /locus_tag="LP543_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBL2321436.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     KNFKNIALDTLKKQLEKQTAVSWTT"
     gene            24867..26321
                     /locus_tag="LP543_001262"
     CDS             24867..26321
                     /locus_tag="LP543_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2321437.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            26337..27368
                     /locus_tag="LP543_001263"
     CDS             26337..27368
                     /locus_tag="LP543_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL2321438.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            27375..28562
                     /locus_tag="LP543_001264"
     CDS             27375..28562
                     /locus_tag="LP543_001264"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBL2321439.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            28718..30100
                     /locus_tag="LP543_001265"
     CDS             28718..30100
                     /locus_tag="LP543_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBL2321440.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            30072..30458
                     /locus_tag="LP543_001266"
     CDS             30072..30458
                     /locus_tag="LP543_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321441.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(30658..31326)
                     /locus_tag="LP543_001267"
     CDS             complement(30658..31326)
                     /locus_tag="LP543_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBL2321442.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8916 a         6393 c         5693 g        10549 t
ORIGIN      
        1 atttttactc attcatagac acttccttaa ataactttta ttatagaagg tattgtaaat
       61 aataatattg acaaaaagtg actcaaaata aaaaaaaact tacattatac tggaatgaac
      121 tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat
      181 tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt
      241 acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat
      301 tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt
      361 tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca
      421 ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct
      481 ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg
      541 acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt
      601 tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa
      661 aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt
      721 aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta
      781 gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat
      841 tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat
      901 gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt
      961 taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt
     1021 cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt
     1081 aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga
     1141 gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc tttcttctga
     1201 cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac
     1261 tcggcctctt ccccaccact aaatggatct tcaatttttg gaactaacgt aattgttcca
     1321 tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa
     1381 ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc
     1441 actcccttaa taaaagtata ctatataaca ctaagtaacg caaaattccc ctttgtaaac
     1501 tatctagaaa attgttttgt ttaaagtata tcttattgga tataaataga aataaattgt
     1561 agccagttca ttttttcttt aactaaaaaa ataatccaag aaacattgaa cctatggatc
     1621 caaaagctac ccaaatacta tagctaaccc cccgctacct tcaattttgc agataaaacg
     1681 ctttgggaga aaatttcaaa gacaacaggc catcctatca tgcccgatta taaacaaaaa
     1741 gctggctatt ccttttgctc tgacaaattt caattgggaa agttcacgaa actagcaggt
     1801 gaagaagttt caactgttcg gatcaaagaa tgcccgcttc aaatagaaac attggttcaa
     1861 cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg agatcgtagc tatttttgta
     1921 gaagaaggga ttttacatga tccaactcat atcaatgtgg ataaatggca acctcttatc
     1981 tataaattca gagaatatac aactgccaac cagtcgttaa ctttgaattt tagattccaa
     2041 gaattcaaag agtcataatg ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac
     2101 gagtaaaaaa caaatagaag ctatacatta gaaaggtcga ctgtgaccct atagatgcag
     2161 tatatgcagc ttatgagtat ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc
     2221 tttttcgttt tttgccgctc tttctttgaa aaatcgatta tgttatacct tctccattac
     2281 taattcaggc atctgctgag taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg
     2341 ttcgatttgt tcttcagtca agacaagcgg cattcggtca tggattggtg cgactgtgcg
     2401 gttaggttca gtagtcatta tgattgattc tgccattttt tcatcctgat ggctacgata
     2461 aaaaccgcca atatacacta ttttttcctt atttgaaaac ataaatttgt ctttttcttc
     2521 attccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt
     2581 tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat
     2641 ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca
     2701 agatttgacg gaaaaacttc tccagctgct aattccgctg cctgcttcgg ttgtttcgtc
     2761 tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca
     2821 cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc
     2881 aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca
     2941 gtgaatggta ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag
     3001 aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct
     3061 gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta
     3121 gaaagctatt ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct
     3181 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
     3241 tatgagcagc tatttttatt tggctgttct tattacttga tgcagggcag ctttttcaca
     3301 acctctccac taaacgatgt tcaatcagct aaccttcggt attagctcaa aaaacggcaa
     3361 aatatgagga actattttgc cgtttgtttc tctaatactc aggtcaaagc gctgatttca
     3421 caacctctaa tacacggtgt tccagaagta gcttcttcga aaaataagcc gaaatttcac
     3481 aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg
     3541 tcacaacctc taattattaa agaacctttt gcgaagttaa caatctttct ttgatcaatt
     3601 gaaaattctg cctaagtagt gatagatcct gtgtgacttt caacaataag atacttccct
     3661 ggatttcagc aatcaacgct ttggcttgac ggtctgcttc ttcatcggat agtttttctt
     3721 cagaaagtgc caggatattt tgtttcgtat acttgaccca ttgattgact aattctagta
     3781 gcaacagacg gtattcttca tccactgtac tcaactcaat aatcaaatta cctacaggac
     3841 agccatgata agtgatctgt gtctcatgat cagcgaaaat ccagtccaac atttcttcta
     3901 atgcttcttc tggctgtttt ccagcattta agatgtcttc gaacaattct gtctgccata
     3961 cgtccatatg cgcatggatc accgctagac aaacttcttt ttttgaagaa aagtaataat
     4021 aaaattgacc ttttcctgta tcagaagctg ctagaatatc tcggattgac gtttcatgat
     4081 agcctttttt ataaataaga tctttggtcg cttccaagat cttttccttt gttttcaatg
     4141 atattctcct ttttcatttc tcatagtcct atattataca gtaaattcat cattttttct
     4201 tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac tataaaactg
     4261 ttctcatata gctagatgtt cattgattca agaactgcga ggacaaaagc aattcctatc
     4321 ttatgatagt tgctctaata cttcctctgt tgtcatcact tgatctgcaa taaagccaaa
     4381 gttcgtcaac gctgcatcca ttgcttcttg gccaggagca ccaattgcat ctgtcgctac
     4441 cacaacattg aatccactct ctaccaattc acgcatatgg gaatcgctac acaagtttcc
     4501 gttcattcca ccaagaatta ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc
     4561 attgctttct ggtccaaaga ttttatgagg agagctgatg atcgtatttt catccaaata
     4621 aggtttcaat tcttcgataa aatcggctcc tgaaccttct tccactgatt catattgact
     4681 ttttctagca aacatctgct gctcaagcat catatgttct cctgcaccgc caaattgcca
     4741 tttatggtca tgtgggtaaa aataatgagg agaaataaag agtttgtatc ctttttcttt
     4801 tgcttgtttt accatttcaa tcatattttc gatcgtattg tatttttcta agatatcttt
     4861 tgtcaaacca aagccaccgc ctgctggttt caagaactcg acttgcgggt cagtgagaac
     4921 tactgctgta tttttattat tgaacatggt ggattctcca ttcgttattt attttaatac
     4981 atcatttaga gcttcactca ttgtcggatg agaatagatt tgatctctca ataacgtata
     5041 aggaagtttt ccattcatag ctagcgaaat caagttgatg acttcatgag aatcttccgc
     5101 atagatactt gcccctagaa tcaagtcagt ttcagggtcg actaaaactt tgaatacacc
     5161 tttaggatct tctaatactt gtgctttagg gacaccgctt gtcatatact tgaataattt
     5221 gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa cctacattgg ataaagtcgg
     5281 agtgataaat acactgtaag gcacagcttt ccggttattc gttgttcgtt tattctcgcc
     5341 cttcaactga tccataatga ttcgataatc atctagagag atataagtga actgcaatcc
     5401 gcctttgacg tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt attcatccac
     5461 agcgattgct ccgcgatctg ttagtttaac atctgtattt tctaatccaa gtgatgctgt
     5521 acttggtttt cttccagttg cagccaaaac ttttgacgct tggattgtta cttcttggcc
     5581 atcttctgta aaggtgacgg cagctgttga ttcttggtct gctacttgat ctacagatac
     5641 acctagatgg aattcgattc ccgcatttgt catgtcttct aaaatcattt gcgcgatatc
     5701 ttcatcttca cgtggaagaa attcagaatg ggcatcaaga acgacaactt tagatccgta
     5761 ttcattgaac attgatgcaa attccaagcc aatatatcca gccccgatga tcactaattt
     5821 ttccggtaat tgttcctgat ccatcgcttg cgtagaatca atcaagtagc gacttgtatt
     5881 caatcctttg attggtaaaa tgattggttc agaaccagta ttgatgaaaa tccgttcgcc
     5941 ttcaacaatc actttttcac cattattcgt cacttcgatt tgatggttag aaagaaaacg
     6001 tgctgtaccg tccatcactg tcccagttgc ctcatcgctg atcatatgat aatttttggc
     6061 gcgaagcatg cccgtcaatt tttctttccg agcaacagcc tctgtaaagt cgattcctct
     6121 ttctccattg aaaatcaatg atttagaagg aatacagccg atgttgatac atgttccgcc
     6181 atacatacgt gttgactttt caacgactaa aaccgattca ccttttgaag ctaacgcctt
     6241 cgctaaagtc ttaccgccct ttccaaaacc gatcacaata ttttgatatt tttccatcat
     6301 ttttccctcg cttctttttt acctaccgat tggtacaaaa atatgataac cgataattgt
     6361 ctattctcca aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc
     6421 acgtttttag tctggttcga tttcctttat tcttctaaac aatcgttact tctacatcga
     6481 tatttcctct agttgctttg gagtaagggc agaattcatg cgcatcttct gcaatttttt
     6541 gggcttcttc ttcgctgatg ccatcgatat gcacttctaa tactacaccg atctgatagc
     6601 cgccatcatc taaattataa agagagacac gtgcttttac tgtggactct gctgtcattc
     6661 ctgcttcttt gatcatatgt tgtaccgccc cattgaaaca agaagcataa cctgctgcaa
     6721 ataattgttc tggatttgtt ccttctgcat ggattcctgg aggagtgatt ttcatatgca
     6781 tcgagccatt tggtgcctcg acttctccat tgcgcccgcc atggttgatc attgctgttt
     6841 cgtatgcttt tttcattgta ctgctccttt cgattgacca tttgctactt ggctataatt
     6901 atagtgtatg acaagtaagg cgctttctac aaaaattctg cttattcgaa taccagaatg
     6961 ccgtcttcta ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata
     7021 tttgttccaa aataaatagc aatcttttct acataatctt ttatatgatt gattttaaga
     7081 tacatatata ccagaaagtt gaccccataa accaatactg ttaaaaaaat aaccatattc
     7141 gttaccattt tttcctcctt ttgctggtct ccctaccagc actcgattta accataccac
     7201 tttttttgcc gtccaagcaa ctagcctttc aaaaagactg gtttattctg ctgttaatga
     7261 ttcaagatca gaaaaatgtc cgaataattt tgctaatttt actaacgcca acttgttctt
     7321 tccgaattca tcattttcta tctgtaatac tagtaacggc tcatgtaagc ttaaacggat
     7381 caaaaaccag ccgtttccgt atggctcttc tagatcatat cgaatacctt cttcattttc
     7441 cgggtcgaag ttcatccctt tttcttcttc caacagtcgc ttcatttctt ctaatatctt
     7501 gtttccttgt tcgcgtggtt catcgctgaa caattttaat cggatttctt gtacttctac
     7561 gggctgtttc aactctttga tttgttcatc taatgtgata ccttttcttt ttaattctgg
     7621 caaaagcatc aggatctttg cgatgacata tgcaccatca tccagaaaat agttttcctt
     7681 gaatgcagcg tgtccgcttg tttcgatggc tagctgtgta tctttcccaa gacggttaag
     7741 ttctaatgct ttattgatta catttcgata gccggaaata taacgaactt ggatgcctcc
     7801 tttttcttca atgaatcttt gtagatgaga agaagtaggg gaattcgtca cgattcttgt
     7861 tcctggatgc tcttttaata cgattgtact taatactgcg atcaaattat ttcgattgat
     7921 cactgttcca gaagcactga caactgcaga acgatccaca tctgtatcga aaatgatccc
     7981 aagatctgct tcatttgcta aaacagcctg cttgatactt tccatcgctt ctttattatc
     8041 aggatttggg atatgattag gaaatcggcc atctggttcc aaaaattggg aacctgttga
     8101 atccgcacct aagctattca atactttttc agcaaagaat ccaccagcac catttccggc
     8161 atcaacaatg attttccaac catttagtgg tttgctttct agtgttccaa tccccttttt
     8221 aatttttgtg actaaatctt gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc
     8281 agtttgttcg ctttttattt tttgctgctg tgataggata aaagcaatat cttctttttc
     8341 tgctcctcct tctgaggtaa agaattttat tccattataa taatagggaa gatggctagc
     8401 agtcagcata accgcagcat cgcacgagaa ctgttcaaat tgagtggaca taaacatagc
     8461 aggcgtagta gctaattcga aatcgatgac atgtatccct tgttctttca gactttggat
     8521 caacgcctct ttcagtgctg gtccgcttat cctactgtca tgaccaatcc caatcgtcca
     8581 ttttttattt ggatcggttg atttcttctt tttctttatc cagtttgcta gtccccagcc
     8641 gatctttttt accatgtctg tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat
     8701 tcctcgaata tccgatccat tttgcagcgc tttcatatct ttcatataaa agtcctcctc
     8761 atttattccc tcattttttc cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg
     8821 caaataataa tgaatgtatc tacaccggcc atcgttccga caacttcagg gtaagccact
     8881 tcatctagcc aattcgttac cacatcggca ccatttcgtt cagtcaatac gataaccatg
     8941 aactgaacag actggatacg caaaacctcg cgtctaattg ctgtacgcaa acgttcttca
     9001 tttaaagaaa cagatggctg gttaaataat gtatagcgta ctattttgtt ttcattttga
     9061 ctttttacta acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg
     9121 cctgcttcat gcagccgcgc aagcagctct tcctgtgtcc caataggttg ttctgtaatg
     9181 atctggcgga tcagtgcttg tcgctgtttc tttttcatat tagacacctc tcaatgttga
     9241 ttatagcaaa agataagtaa tatttcttca tattttggta ttttttactt ttattttttg
     9301 ttttcattca ggttgctaac ctttttctat tttaacacgc aaaatcgtct tcattttttc
     9361 aggtgctgat tttcttggat cgctgatgta aatctctttg tgttctttag aagctctttc
     9421 atagccttct tttgcgatga atgcttctaa tttctcaaac gttgctggtt cttcggcata
     9481 gggcccgacg tgtaagattt gggcacaaag accttcttcc atctttttga atactacttg
     9541 ttctttcaag tcttccggaa gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc
     9601 tgtaacaaag tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt ttttcatcac
     9661 tggttcatgc aaatactttt cttgcagtcc ccattgtcct tccagtggat agacagtata
     9721 catctgataa tcaggaatca gccaattttt tttaggcgac atgcgtattg catagctgac
     9781 tgcatataga caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct
     9841 gatgctaaag aatgattttt taggaacctc tagtatttgc gggctttttt tcatactata
     9901 gctcttgtct ttacgccatt caattttttc catttttctt cctccgcgaa attccattta
     9961 gttatttatt atcatacctc tttttgagtg acttggctaa tcgtgttgac actatttttc
    10021 agtttgtgct agaataagtg aagtaaataa atcgtacaga taacacgttt cggcgtgtcg
    10081 agagagaggc ggtttctatt tttataatag aaatcactct ctttttttac gaaatttttt
    10141 agaggtgatg taaatgacaa aacaaaaaca gctgctttta gtacctgcag taattgcaac
    10201 agggatgatg tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa ctttcccaac
    10261 tcttatcaga cagtttgatt taacgactgc acaagtccaa tgggtaacaa cgatttatct
    10321 tttgatgatt tcgatcattg ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa
    10381 aaaactcttt ttagcttcaa atctgctttt cttggcaggt ataatgatcg atttcttctc
    10441 tcctaccttc atggtcctat tgtttggtcg attgctccaa ggtgcagcga caggtatcgc
    10501 tcttccgttg atgttccata ttatcttgac atttgccccc ctcaatcgac gaggcgcgat
    10561 gatgggtgtc ggtactttga caacggccat tgctccagca attggcccca cttatggagg
    10621 cattatgact tcacatttta catggaatca tatcttcctg tttcttgtgc ccgttttgat
    10681 tctttctttg attatcggct ggtttgctat tcccgagatg gcagtacaaa aaagtggtta
    10741 tttggattgg ataagcgttt taggaatttg tcttttgttt agtggaagtc taacattttt
    10801 gagtacgttg ggaacagttt ccagctggat tgctttaatc atcggcggta ttggctggtt
    10861 ccttttttac tatcgaagta aaaaagtgga gcatccactg atccgactgt ctattctttc
    10921 aaataaaaca ttccgcttgt ttttgattgg cttcttagtt tatcagtttt tattacttgg
    10981 tgtatctttt gtcttgccga atttccttca gatcgttcaa ggaagtagtc cttttgtcgc
    11041 tggattagcg atgcttcccg gcgcagcggt tggtgctttg ctttctcctt tttccgggaa
    11101 aatgctggat caatatggtc cgaagaagcc aatttttgct gggctgattc tttctttgat
    11161 tggttggcta gcattattcc tactcatagg acacgcttca gtgggtgtat tgattgcttg
    11221 tcatgtgttt tatatgatcg gtatcggatt atcttacagc aatatgatga ccaccggaat
    11281 gaatgcactg aatgaagaac tacaaggaga cgggaacgca atgttcaata ccttgcagca
    11341 gttttctggc gctgtggcta cttcattagt agcggtcatt atcaattacg tccagcatca
    11401 tacttcccat aactatgaag tgtctactac attaggatca aaagcagcat taggcgtctt
    11461 gttgctgttg cttctcgtaa gctttgctcg attcgcctat tatctgttct ttgctaaaaa
    11521 agcgtagatt ccatatacgg caggtgttgc atctgccgtt tttttgtgtg aaaatggttg
    11581 tttgtaaaag gaggcttttt tatggaaaaa attgccatta tcggtggcgg gatcattggt
    11641 atgaccctag ccagttactt agatacatca aaattcaatg ttgctttatt tgatgaaggg
    11701 acaggacagg caactaaagc tagcgctggg attatctccc cttggttgtc gaaacgcaga
    11761 aacaaacaat ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa acttataaaa
    11821 gatttccaac ttgatcaaac gatttacgca caatcaggga cacttttgct tcgacctgaa
    11881 aaagatttag ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt
    11941 ggaaacatcg aatggctttc acctgaacag acatctcaag cacttccgtt attgaaacca
    12001 gcagtttcac tgcgtatcag tggtggtggt cgtctggatg gaaaagcata tttagaacat
    12061 cttggtcagc gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt cactttgcat
    12121 aagaagagcg attcttggct catcaaggga ccgtttggag aatacacagc tgatcgtgta
    12181 attttgacac ctggcccggc acttaagcct ttattacaag aattaggcta tgctgcagat
    12241 atacgtccgc aaaaagggca gctcttagca tttcagacat cttataagat gagtcaagac
    12301 tggcctgttg ctatgcttga cggagaagct gatttcattc ctttcatgga tggaaagatc
    12361 cttttaggtg ctacccatga aaacgatcaa ggctgggatt tggctcctac tagcgaagct
    12421 tataagggat tgactaagag tacagaacag ttcttgactg atccagatca gataaaaaac
    12481 tggccatacg agttccgtgt aggtacccgt gcttatacgt cagatttttc cccatttttc
    12541 ggcgtactgc cagatgatcc aagcttactg gctgctagcg gtctaggctc ttctggcttg
    12601 accacaggtc cttatatcgg ctatctatta gccaacttct taaatactgg tcattggcct
    12661 acggaagatt atcaaaagga catctccacc tatattacga aaaatccatc atagaaaaac
    12721 agctttttca agtaaggatc ccgagcgata ttgcgcatgg attacttctt gaaaaagctg
    12781 ttattttagt tcaatttctg aatcaaattc tccaaacttt cttttgcttc atctggcaag
    12841 agatcttttc ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc
    12901 agatttttat aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg
    12961 atgtcttcaa atggaccaaa cgacttgaat tctgccgttt gttgcacgat tctttcaacg
    13021 attgacaatg tcgtttcagg tttgattttt ttatcatcga aatatccagt attcaggaca
    13081 tagcaggctg tttgctgttt caaaaacaac tctcgaaaag cttgatagtc ttccctaaga
    13141 ggatagacac ggaaaggatt agcaaatggt tcaatgacta gctcatctag ctgtcttttt
    13201 gtagtgatat tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct
    13261 aaaactgggt cattgatctt gataattggc ggaagcgaat catctttcat gatccaaaaa
    13321 acagcatcga tcttttcgct aagatgatcg actcggtcgg gtgtcgcata acgtgatttg
    13381 actgtccgcc catttccgtt tcgtatatct cctgttacta ggactttttc tcctttactt
    13441 gtacatgtca cgcccacatt ctgacaagta aggaaagaag aaatggccgg atcatcagac
    13501 ggatagtctt gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa accatttttt
    13561 cgagaaataa cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt
    13621 gctaaggtaa tcgttgattt tccagaaccg gacaacccaa atacagccat cgtataggtc
    13681 tggtcattac tagcatattg tttcatccca ccgtggcagg caataaagcc attgcgattt
    13741 cccaatgccc aagcaagcgt caaagtcgct ttttttaatt ctccaaagta acgtaatcct
    13801 agcacaaccg ctgtattatg gagagcatca aacaaaatga gtccatttgg aaatgtttca
    13861 tgtgtccatt cgggatcaca aaagatataa agatccccct catcaaagga aacagacttt
    13921 tgataagtct ctaagatctt cgaattcgct ggctgaaaat tcagcaaata agaatacaaa
    13981 ttgttctcaa atccttctgg aatcagcaaa tgactttgca cagaaaattc ttcagatagg
    14041 ccaacgatta cttttgtatg gtaaaactgg cgcttgcgac tttgatacac agcttctctt
    14101 aaaattttct cgtatttgct ttgttccttg tctaagtcct caaagatccg ccgagcactt
    14161 gctgttcttc ccacaatgct tccatcattg aatactagtg tcctaacatc agctggtaaa
    14221 tctagttctt cagcatgtga gattgttaaa tctgtgatga tcgttcctgg actccctttt
    14281 gctagttgat aagctgtctt cctatcaaaa acttcatgaa cattattacc gtaaaatgtc
    14341 gtttctacaa tcgatcgaat actggataac agtggatttc cacttgtgat ttcgtttaat
    14401 tcgagactct cttttgtact catggctcct cctcattccc taaacaaaaa aacgttttct
    14461 ttattataca ttattcgagt ttttatttgt actgctgcgc tgtgggaagc aaacgagtta
    14521 tttttcacac aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc atcattttcc
    14581 ttttatcggc ttagtaccag ccgtttgcta accagaatgt tttagcagca tcccatgaac
    14641 cataacgaga tgttacatat tgttctgcta cacgttcttg attggcagct gaatagtcgc
    14701 cgtttaagta tgaagcatct aattgataac ggccgatgta acgtccgtta gtagctgtgt
    14761 atgaaccgcc tgattctttt tgtgcgatcc attcttttgc agagcttgaa gttggcgctg
    14821 ttgcagcagc tggggcagca ggagctactt caactgtttc aacaggtgca acgtatgtat
    14881 gttcttgcgt tgctgtttca gttgtagatg ctgtattttc tgttgttttg ttttctgttg
    14941 ttgctgaaga atcatttgaa gttgggattg ttaattgttc accaacataa atgcggttga
    15001 tgtttgcgat cgaatttttt tcagcaattg catcgatcat tgagttgtct cctgcaaatt
    15061 tttgtgagat cttagataat gaatcacctg attttactgt ataaacttca tcagcgtgag
    15121 catttgtagt tcctacaaac attgcagtag caccagcagc aaaagttgta cctaaaacaa
    15181 ttttcttaaa tgaattcata ttaaactcct ttttttctcc gttttttatt ctttcgacgc
    15241 tattaacttt atcatttaga aatatttcat aggtaaaaga aacatgacat ttaatgacaa
    15301 aataacgaat tatttaatat ttgtaacatt taaacaaata tttttttgat acaaaaccat
    15361 aaaagccttg ttgtataagc aaagtaatcg tttttctatg tttttttatt tttgaatgaa
    15421 aaacgcagat tctgtaacat tcactgcaat aatccataaa aaaaagcaaa aaaatttttg
    15481 atttttatca aattctctta aaaaaatgta aaaaatacaa aaaaaccagt aattgttaca
    15541 acaattactg gacaaaaact ttgaataatg ttaactattt ttttatttta actttctccg
    15601 caataatttc tgggcatgtt ccaattcgtt tataacgact tcttctgttt ctttagctaa
    15661 tatttcatct aatacatcaa gccatttttc cggttctttt gttcctagac gtccaatagc
    15721 ccaagctgct gttcctctga tgacagggcg gacatctgtt aaacattctg aaatctcagg
    15781 caatgccgtt cgatcaccta gattagctaa agcaatcaat gcgtttctct gcaaaggttt
    15841 cttcccacgc catgaaccag ctaaatgccc aaattgctgt ttgaagtctt tattagacat
    15901 cgaaagcatt ggctttaatt ttggatatat ttcgtctact tttggttcca tctcttcatg
    15961 aaaatgaaaa tcttttcctt gattgtaagg gcagactaac tggcagatat cacatccata
    16021 aatgacattt cgcatttttt tacgatattc ttccggcatc atcccttttg tctgagtttg
    16081 ataagacaaa catcgctggg cattcattct tccgtcacca agcaatgctt ttgtcggaca
    16141 agcagtcacg cagcgcatac agtcaccgca accaaattct cctggtttat caggctcaaa
    16201 tacaatatta gtaacgatct caccaagata gacaaatgaa ccgaactctt cagtgatcaa
    16261 caatccgttt cgtccaataa atcctaaacc agctcgctgt gctacagcga catcgatcag
    16321 ttctccagta tctacttgcg gagctagacg ccattcctct tctttttgcc atttttctgc
    16381 tcttgtttgg atgaattcga tcaatcggtt caaacggtct ctgagtatat catggtaatc
    16441 gatcccccaa gaagcacgag caaactgccc gcgtttttca tccctaggca cttcttcatg
    16501 gatcctagta ggatacgcaa gtgcaatgga aatgatcgat ttcggctgat caaatgtcat
    16561 ttctggataa agacgctcat cgatatttgg atgttcaaag ccagaagtat ggccagctgc
    16621 tttttgttct tccaacgatt cttttaatga atcaaaaggt tccgccgtgg tgaacccgat
    16681 tttgtcaatt cctaatcgtt tgctttcttg tatgatttct tcttttaatg tcatggtctt
    16741 cctcgcttgc tgttaacggc tgtataaccc ataatatagt ttttcttttt tcagctggac
    16801 aaactggatc tgctgatcgg caatcttctt ttcttccatc catttttttc ttgcagcaaa
    16861 tacttcccaa taatttgttg aaattaattt tcttttgtta tctaatgcat taaaaaaatt
    16921 caatgaaact ggttttgtac ttaacagttc atggagcctt acctcttccc atggcgaaaa
    16981 ctcagacaga tcgcgggaat acaatacttc ttcatagaga tttttttgct gtgtgtacgt
    17041 gaatgtttgc gggattttcc caggcctagc aatttctact agacgattat aaaacatatt
    17101 cattaatttg ctgttggcaa taaattgact tttataatag gccgactgga tctttttgat
    17161 tactccttct gctttgatga ctgaaaataa tgcaggccat gctttgacgg ttatttcgtc
    17221 gttttgttta actaatacgc caggtgtgaa gccgactgga gaaaggaaca caaaataacc
    17281 gataccggta ttttcatcta aaggctgaaa ttcatcaaac aaccatttgc ctatgtacag
    17341 ctcttcatct ggttctgttg tctcattttg atttgctatg aataatttag ctaattctgc
    17401 ttcaagaaat tgaaggtctt gatccttgtc ggtattttcc tgctggctga tcaatcgaaa
    17461 gacttcttct ttaatcgtat atttatcttt acgtgtaaaa ttcatagcca caccttcctt
    17521 ccttactcta actataacgg aatttcttca aaaaaggtat ctgtacgacg aaaaacagca
    17581 taattttttt tcatatctct tataaaaaat gcaaggtctg ctcctcaagc tagaccttgc
    17641 acttttcagc tttcttttgt ttgtgacaaa ccttcatgga tcgtttcaat attccatttc
    17701 atcatctgat agtaagaatc tccttgttct ccttcttttg ctatggaatc agtgaacagt
    17761 ttatcataga ttttcaaacc cgtttctttt gctacccgtt ccatgcttct tgagtctaca
    17821 ctggtttcca cgaataaaac cggtacttct tttgctctta tctgatcaat gatcgctttc
    17881 atttgatcag gcgtaccttg actttctgta ttgatctccc atatataagc tgctggcaaa
    17941 tcatatgctc gtgaaaaata cttgaatgcc ccttcacttg ttactaataa ttttttgttc
    18001 tcttcgatag aagcaaaact ttccttcgct tgtgtatcta attcttttag tttttccaca
    18061 tatgcttctg catttttttt atagagtgtc gcattttctg catctttttc agagaatata
    18121 cgtgcgattt cctccacata ttttattccg ttagataggt ctagccaagc atggggatct
    18181 gcttttgtat gttcttcacc gctagttaaa taaagaggtt ctacattttt gctaactgca
    18241 aaataatctt ttccttcttc ttttttagcc gtttccatca agttatcgaa ccagctgtta
    18301 cctgtttcaa gattcaaacc gttgtataaa ataacatctg catcgcttgc ctttttgatg
    18361 tcttctggta gtacttcata ttcatgcgga tctgttccga ctggtacgat actgtggata
    18421 tctatttgat ctgttccgac ctcttttgcc atgtccgcca agatcgaatt ggtagccacg
    18481 actgacaact tttctttact tccttcatcc gcttggtcag aagcctgctt tccgcaaccg
    18541 gctaatacca agaaactaac tgccaagatt aaaaagagtg atttttttgt tttcatctat
    18601 ctgcttcctt tctgaaataa aattcctttt gttggagaaa ataaaaaagc aaggataaaa
    18661 aatgcggtac atgctaacac gatcgtagct cctgatggcc aattgtaact ataactgaaa
    18721 aacacaccga caactgcact tagcataccg attcctgcag ctgtaatgat cattttcagt
    18781 aaatggttcg ttagcaagta agccgtggcg gctggtgtga tcaacatggc aatcaccaag
    18841 attgttccta ctgtctgtaa actgactaca gccactaatg tcaaaaagaa catcaaaaga
    18901 taatggatca gcgatgtgtt cagcccataa gcttttgcca ttgtcggatc gaatgatgtg
    18961 atcttcaatt ctttataaaa gaaaaagaca aatatcaaga cgattccact aacgagtgct
    19021 gtcagcaaca aatcgctttc cctaacagct aagacattcc caaataaaat atgatacaaa
    19081 tctgtcgcac tctgggcata tgaaatcaag ataatcccta atgcaaaaaa ggagctaaag
    19141 acaattccaa tcgctgtatc atttttcaat cggctccgct gagtcacgaa accaatcact
    19201 gctgctgtta gcatcccaaa agcagtggct ccaaagatgt aactaaaacc aaacatataa
    19261 gaaatggcta cacctggaag aactgcgtga gagatagcgt ctcccatcaa agacatccct
    19321 cgtaaaataa taaaagaacc gatgactcca gaagcaagtc ctacaagcat ggaagtgacc
    19381 aaagcgtatt gcaggaattg ataattcatt aatccatcaa taaaggattg gatcattttt
    19441 tctctcctcc ttttactaaa tgtctgatga tctcaccata ggccaattga aggttcttcg
    19501 atgtaaagac ttcttgtact ggaccggaag cgatcagctg tttatttaaa agaataacct
    19561 catcgaaata ttcttctact ttatgaagat catgatggac gatcaaaatc gtttttcctg
    19621 aattcttcaa ttcctgcaag atgtcaaaga tctttcgttc acttaacgca tcaatcccta
    19681 cgaatggttc atctaaaaag atccattctg ctccttgggc tagagctctt gcaataaaaa
    19741 ctctctgaag ctgtccaccc gatagttcgc tgatctgtct tttggcatat tcttccatcc
    19801 ctactttttt caaagcttgt cttgcacgtt ctttttcttt tttcccaggt ctttgtccaa
    19861 ttcgtaaaga tggatatgtt cctaaaagta caacgcctaa tacatcaatt ggaaaggaaa
    19921 gatccaattc acttcgctgt tccacgtagg ctattttttt acggactgag tttaccgact
    19981 gtccagaaaa ggtgacttta ccagtagtag ctggtatcaa ctctagcaaa gatttcataa
    20041 atgttgattt tcctgcaccg tttggtccga taataccggt aattttttgt ggatttatcg
    20101 tcaagttgat agttttcaaa acagttttcc cttgataagc ggctgaaagg tcttgaatta
    20161 caatggccgt acgttccaaa tcttacactc ctttttaggt ttacctaact ttttatttaa
    20221 atatagttta actaaaatgg aaagcatttt caagtagaat gatgaaaaag tttttttcga
    20281 attttcctcg cattttatga aaattttctg tagaatcaca tcaagagttc aataaggcta
    20341 acctaataat gaaaaacatg tgttgaaacg tttgattatt ttttcttgtt gactcaacaa
    20401 cgaaaggagt ttttgtatgg aagaagaaaa gttaactcga agtcttagtg cccgtcatat
    20461 ccaaatgatc gcactaggtg gaacgatcgg tgtcggtctg tttatgggtg cctcctcaac
    20521 catacgttgg acagggccct cagtgatggg agcctatgcg attgcaggat tctttttgta
    20581 tttgattatg cgtgctttag gcgaaatgct ttacgttgat ccttcgactg gttcttttgc
    20641 aaattacgcg agcgagtaca tccatcctgt ggctggctac ttgaccgctt ggagtaatat
    20701 ttttcaatat attgtcgtag gaataagtga agtgattgct gtaggatctt atatgaatta
    20761 ttggtggcct gatttgccgg caattattcc cggaatcatt gtcgtcttgt ttcttatgct
    20821 ggcaaatctt atttctgtca aggcgtttgg cgaattagaa ttttggtttt cgatcatcaa
    20881 agtcataacg atcattttga tgatcattgc aggtcttggg gtaatcttat ttggatttgg
    20941 taatcatggt caggcagtag ggatttcaaa tctttggaaa aatggtggat tcttcactgg
    21001 aggcgtcaaa ggatttttct ttgcattatc gatcgttgtt gcttcctatc aaggaatcga
    21061 actgatcgga atgactgctg gtgaagcaga gaatcctaaa aagacgatca ttgaagctgt
    21121 ccagtccact atcggtcgaa tcttgatttt ttatattggt gctattttta tcattgtgag
    21181 tatctatccg tggaatcaat tgagtgagat tggttcacct tttgtacaaa ctttttctaa
    21241 agtcggcatt acatttgctg caggactgat taattttgtc gtgattacag cggcattatc
    21301 aggttgcaat tcagggattt tcagcgcaag tcgtatggtc tatacacttg ccataaatgg
    21361 aaagatgtct aaaaaatttc tgaaactatc tcgtcacggt gcgccttttt atcctgtcgt
    21421 agcaatctca ggtggaatct taattggtct gatcttaaat tatcttctac caatgttcat
    21481 caaccagtca gaaaatattt tcgttttcgt atacagctca agtattttgc ctggtatggt
    21541 tccttgggta gtgattttga tcagcgagat tcgcttcaaa aagaagcacg cagataaaat
    21601 gcatgatcat ccttttaaaa tgccactatc tccttatacg aattatgtga cacttgcttt
    21661 tttagctatc gtattgattt tcatgtttat caatcctgaa acgagaatct cattgattat
    21721 tggactcgtt ttcttgcttt acatgacgat ccattacttt gtacgcgaaa aaagaaccgg
    21781 aaaataaaaa aagaacgaag cttcacctta gtggaagctt cgttcttttt tattgctttt
    21841 atcaattcaa ttttagcgtc tcaccgggag tcaggaaata attctccatg ctcatcccat
    21901 tcagcgacaa gatcgtttct actggtacgc cagttcgttc ggctatttga tttggacctt
    21961 ctccttccaa aactacggtt gtcgcagcct gtgaagtcgc gctgttggta ttagtttcag
    22021 aggaatctgt tgtgtcttgt gttgttcgag cttgcatttc agaagcttct accgactctg
    22081 ctgtctcaga atcagaagat gttgtgtctt gtgtgcttgt tgactctgct tgaagagagg
    22141 cgctagtctg caaataaata tcggaaatct tagcgacatg attcgtataa ttagccgata
    22201 attgattgat caaagtatct aagtctacac cgtctttatt ttctgctgcc tgatacgctg
    22261 cacgtaattt tctgatccct tcgtcggaga ttgcttgaag ctcacgtgct ttttggttag
    22321 caactgctga taaaccagct attccctctt gatttccctg aatttctgtc tggtattctt
    22381 caatcaatgc aggcgtcttt tcttcgatct ttttcgaata atcatttacg attgcttgaa
    22441 gttgctctgg caaaccattt gctgtttttt cttcagaagt ttgtgtctct tttgttttag
    22501 ttgatgactc acttgtttgt ttcgtctctg ttttctttgt cgttgtttcc gttatggtcg
    22561 tacttttcgc ttctgacgaa ttttttccat gaaaatatag acccgcaact cctcctgcta
    22621 tgatcaaaaa cacggcaact gctgccccaa tcatgatcat ccgcttacga gctgcctgtt
    22681 tttgctgtct tcttcgggtc atccgaacat tttctcccca ctcattttca tcatacattt
    22741 ttttcaccac caatttttat gtataatagc gttatcaaac gcttcatatt cttattgtag
    22801 caaataattg tcgctgtgca catttttatc tttgttattt ataagaaaat ttcaccagtt
    22861 gagatgattc caagattgtc cgtcgatttc tccttgcagc tgcattgttc gttcgtctat
    22921 ctcagccatc agtttcttta tttcatataa gagcgcacgg tcttgatatt ctgatttttc
    22981 gcttaattca tctaattgtt ggatcagcca atcatttgtc ataatgaagt tcctttcatt
    23041 tcttgcagtg taaaggtcaa gtcttcgatc tgctcttttg cctcagtcag tgcttctacg
    23101 atatatgctt gtttttcaga ttcttcttcc tgcattttct gattttcctg tactctttga
    23161 ataaaccagt caatccgctg agtcagcgat ggtttgcctt ccaatgagag atactgctga
    23221 taagcaatta gcttctccca acgtttttcc tgggataaat acataaattg tgctaaaacg
    23281 aatggcggaa gataggtcat atggaattta tttgcgattc gctgatacgg cctcaaaaat
    23341 tcggaaatcg tatattcttc ttttcctcca gcttggtatt ctttttccgc cacacctgtt
    23401 gtcaatacga ttcctagttc tttccctttt aatacatgtg ctgctcgttc caagacatta
    23461 tcttgccaaa tttttaaatg agcaggagaa ctataccagt aaaacggaaa ctggaaaatg
    23521 atccgatcat gctgggccaa gagctgtagt tcagtctcta tatctatttt tccgtctgga
    23581 taacttgatt ccaaatgatg ccaagtagct ggtaaaccag cagaagcttc tttgaaaaat
    23641 tgctgattta aagattcttc gatcgttgga tggctaatga tgataagtgt acgcatccct
    23701 tttcctcctt tttagtcttc gctttacagt gtagcataac cgccacaagt ttcttttgcc
    23761 gatgcttgtc attttaacca gactaatcag gttgtccaag aaactgcggt ttgtttttcc
    23821 aactgttttt tcaaggtatc caaagcaata ttcttaaaat ttttgttttt catacgttta
    23881 cgatagttcg tagccgtttc tccggtactc tttttgaaca ttcggctaaa tacgactaga
    23941 ctattgaacc ctacttgata ggcaatactt tccacggatt catccgtcag agaaagaaga
    24001 aacgcccctc gattgatccg taccccgatc aaatattcct tgatactggt acccatatga
    24061 tttttgaata ctcgcgacaa atggctccga ttgaccgcta aatgctgggc gatttcctca
    24121 acagacaatt tttcagcata atgtgcctcg atgtatttga ctgtttcaac agctaaagag
    24181 gaatgaacct tttgtacacc accgctgacc tttccgccat cttttaaaag gactgataaa
    24241 aaacggtagg ttaatgctgt taactgtaat tcattttcca aggtttcttc cgtatctttc
    24301 atgcattcta aaataatgtc gatatatttt tgtatgtcag aagagaccat cgtgtattgg
    24361 tcttctctga aaagcgactg ccgaatgatt tcttctgcag cactcccacc aaatgaaagc
    24421 cagcaataca tccacggatc ttctccatct gataagtaaa acgtcgagtc tcctggacga
    24481 atcaagaaca gatcaccttt tttcaattgg aattgttgat ttttcacatg ataagatcct
    24541 ttgccttcca tcacgacatg caggatgtaa tcattgcgaa tagcaggacc aaaagaatga
    24601 aacggcatcg ttttagagaa accacaaaaa tcaaaataca attctttttc tacttgttga
    24661 tgttcctttt ttaaaaaaat cgattgctcc acgtctatcc ctccttactc ctttagtata
    24721 atgcaacaaa aagttaaata aaagtcacag aaatatatta aaaaggaagc gatttcttgt
    24781 taagtttaag tcattaaaag atctttgtta ttcgttgtaa ccggtttact tagtattata
    24841 tattgaacag gagaggaata cagattatga aaagcaaaat gatccaacgt gtgtcctacg
    24901 ctttaggcgc ttttggacac gacacattct atgttacatt aagcacttat tttatggtat
    24961 tcgtgacaag ccagatgttc gcaggaacag ataaggcaac gaatgcaaaa atgattgcta
    25021 cagttacaag cttagttgta ggtatccgct tagttgaaat tatttttgat cctttgattg
    25081 gtggcatcat cgataatact cgtacaaaat acggtaaatt caaaccatgg ttagttatcg
    25141 gcggcgttgt tagttccgta ctcttggtag caatctttac caatttcttt ggcttagcta
    25201 ctcataatac gactttattt atcatattat ttgcaatcgt tttcatctta ttggatagtt
    25261 tttattcatt taaggatatc gctttttggt caatgattcc agctttgagt acggaaacaa
    25321 aggaacgtga aaaatttgct acgtttgctc gtttcggctc ttctctaggt gcaaatggta
    25381 ctactctagt cgttgtccct atcgttactt tctttaccta tcttgttact cataaacata
    25441 ttgaaggcgc ttcaggctgg ttctggttcg gtgtgattgc tgctgttatt tcaggcggta
    25501 ctgcgattat cactgccttg ggcacaaaag aagtcgatac tgcgatccgt cagcaaaatg
    25561 aaaaagctgg gatcaaagat gtgttcaaag cagttgctca aaatgatcag ttgatgtggc
    25621 ttgcccttgc ttatatcgct tatgcgattg ccaatgcagc aacaactgcc gtattattct
    25681 acttcttcaa atatgtactt ggccaaccaa ctgaattttg gatcgttggt ctagttgctg
    25741 ccttacttgg tttagtagct gttccactat tcccaatgct tacacgcgtc atctctcgtc
    25801 gctacgttta ccttggaggg atcgcaatga tgatttcttc ttactttttc tttacgatcg
    25861 caggttcaag tttgccaatc gttatcatcg ggctagtatt gttctacttc cctcaacaat
    25921 tgattttctt atccgctttg atgacgatca cagactctgt tgaatatggt caatggaaaa
    25981 atggtatccg taacgaagct gttactctat caattcgccc tttactagat aaaatcgctg
    26041 gtgctttgtc taacgggatc gtagggtttg tcgctatcgc ttctgggatg actggtaatg
    26101 ctactgctgc tgatatcact gctcatggcg taacgacttt caaagcttat gcattctatg
    26161 ctcctgctgc tttgatgatt ttctccgcta ttatttttgc ttggaagatt acattgacag
    26221 agaaaaaaca tgcacaaatc gttgagaaat tggaaaacaa attggctcca gctgatacaa
    26281 tggaagatga acttcacgat gcagtagttc cttcccatta agaaaggatc ttgaaaatga
    26341 agattacaga aaaagatttt ggccaaggtt atcacttgat aaccttggag aacaaaaaca
    26401 agctagcact ttctatctct gatttaggtg ctagaatcgt cagtttaaaa tcaaatgatc
    26461 gagaattagt tttgggattt gacaccgctg aagaatatat cgaaaaagat ccgtatatcg
    26521 gtgcttctat cggtcgcacc gcagggagaa tcgaaaatgg ccacttttct ctcaatggaa
    26581 aaacttacca attagccaca gatccaaaaa ctggacataa tctgcatggt ggtgctcctg
    26641 gatttgaatt aaaaaaatgg tcttatgtca ttttgaatgg cgaaaatgaa gcttctgtca
    26701 tttttactac tacaagtccc gacggagaac atggttttcc aggtacaatg gatgtggaaa
    26761 tccgctatac tttgacaaaa gataatatct ggcgagtgac ctctcgcggc accagtgacc
    26821 aagatacttt attcaatccg acaaatcatg tttacttcaa cttgacggga gatgcttctc
    26881 agtcaatcga tcaacatgag ctttggctca atagcgaagc ttatgctcct ttgcgaacag
    26941 atagtattcc tataggggta aaagaaaatg ctgcaggaag tgctttcgat tttcaaatac
    27001 ctaaaaaatt agcgtccgtt tttgctagtg atttggatca aaaaaatctt gttgacggaa
    27061 tcgatcatcc atttttctta aaagaaaccg gcttgggtaa agaagccgct cgcttgacta
    27121 gccccgataa aagaatccaa gtagatattg ctacagatgc ttctagtgtg gttattttca
    27181 ctgcaaattt cggcacagaa acaccagaaa tgcgcaaccg aaaactggca catcacggtg
    27241 gcatcacatt tgagactcaa acagctccag gtgcagagcg cttctcagca tttggttcga
    27301 ttcatttaaa agccggatca gtatttgaaa cagtgacaga attcaaaatc aaaacaagaa
    27361 aggaataact catcatggaa atcaaaagaa gcttaaacac aaaatttgcc gaaacattcg
    27421 gacctaaaaa aactacacaa tacttctcac caggacgcat caatttgatt ggtgaacaca
    27481 cagattacaa tggaggccat gtcttccccg cttccatcac ttatggaacg caaggtgtag
    27541 cagcacctcg agacgataat aaaatattag tttattccac aaactttgac gatatcggtg
    27601 tcattagttt tactttagat gagctagaat atgataaaaa agcaaactgg gcaaattacg
    27661 taaaaggaat ggtccttaaa ttaaaagaag ctggacactc aatcgatcat gggtttgagt
    27721 tattagtgga aggaacaatc cctaatggtg caggactttc cagtagcgct tcattagaat
    27781 tattagtagg tgttgtatta gaagatctgt ttgatttaaa aacagatcgc ttggaactag
    27841 tccaaacagg taaaaaagta gaaaatgaat tcatcggtgt caattcaggt atcatggatc
    27901 agtttgccat cggcttcggt gagctagatc atgcaatctt attagataca aatacgctga
    27961 aatacgaaat ggttccagtg aaactagatg gttatgcaat cgtcatcatg aatacgaata
    28021 agcggcggga attggcagat tcaaaataca atgaacgccg tagcgaatgc gaagaagcct
    28081 tggcacgttt gcaaaaaact ttgcctatct cttctttagg agacttagat gaagaagaat
    28141 ttgaatcaac tattgaccaa atcggcgatg atacattgat acgcagagct cgccatgccg
    28201 tttctgaaaa tcagcggact ttgaaggcaa aagctgaatt agaagctggg aacttagaag
    28261 catttggtca actgttaaat gattctcacc actctcttcg ctatgattat gaagtaactg
    28321 ggatcgaatt agatacacta gtagatgctg ctcaaaaaca agaaggtgtc ttaggtgcac
    28381 gaatgacagg agctggattt ggtggctgtg cgattgctct tgtcaaagaa gaaaatatac
    28441 ctgccttcaa aaacaatgtc tatgatgaat acatgaaagt tgtcggctat gctccggaat
    28501 tttatgttgc acacattgga aatgggacaa caaagatcga tccagaagct gttcatgttt
    28561 gataaaaaaa tcaacgatct cagctaaaaa caaatagata aaaataacct ctcagcaatt
    28621 tccaaatgtg agccttttgc tcgtatttga aaattgctgt tttttattga cacggaaaga
    28681 gaacgtgtgt tctaattgtg ttgaagaggt gattgttatg tgggcgaatc aaaatccggt
    28741 atttgtttat gaaaaagaac cttcaagaga tattttatgt attgactgca aatcttttta
    28801 cgcctccgta gagtgcgttg aaaaggatct tgatcccctt acaactaaat tagtagtcat
    28861 gtcctatccg tccgattcga cagaaacacg gggtagcgga ttaatcttgg cttccagtcc
    28921 tgcagcaaaa aaagcttacg gcatcaccaa tatcagccgt gccagagatc ttcccttccc
    28981 ctatccaagt gatttatata ttgttgcccc aaggatggcc tattatatgg aaaagaacag
    29041 agaaatcaat tctatctata aaaaatatgc agacgaagcg aaccatcatg tctattccgt
    29101 cgatgaaagc ttccttgatg taacaaatag catggcactc tttggtaaaa caaatgcgta
    29161 cgacatggct aaactgatcc ataaagatgt gtttgagcag accggaatct acacgactat
    29221 aggaattggc gataacccgc tacttgctaa actagcctta gacaatggct caaaagacgg
    29281 aaagcaaatg tttgctgaat ggcgttatga agatgtacca gaaaagttat ggaaaatctc
    29341 agatatcact gacttttggg gaatcggcaa acgaacagca aaacgtctga aacaaatggg
    29401 tatacattct gtttatgatt tagctcacgc ggattattat cacttgaaaa gcaagctagg
    29461 ggtcatcggt actcagcttt atgcccatgc ttggggcatc gatcgaagtt ttttgggaca
    29521 agaatataca ccaaaatcaa aaagtatcgg aaatagtcaa atcctagata gagattatac
    29581 aagacgagaa gaaatcgaga tcgttatcaa agaaatggcc gaccaagtcg gcactcgctt
    29641 gagacgatct gcagcaaaaa cacaagtagt cagcttatgg gtaggatact ctttaggcta
    29701 tcttgatcca aatggcaaga ccgggtttca ccagcaaatg aaaatcgaag cgacaaatgc
    29761 tagcagagat ctagcagatg ctttattgac cattttccac cagcattacc actaccagga
    29821 tattcggcgt gtaggcgtga actgttccga cctcgtatac gcgacaggtt tacagcttaa
    29881 cctttttgaa gatcctgaaa agcagataaa tgaagcgaaa gttgattttg ttgtcgatac
    29941 gattagaaaa aaatacggct ttcaatcaat catccatgcg catagtctct tgcaaggagg
    30001 acgcgccatc gcacgtagtt ctctcgttgg cgggcatgca ggtggtatga gtggattaga
    30061 aggtggaaaa gatgagacgg acagcaaaaa aattcattga ctataacgag tatcgagatc
    30121 ggccatttgg attaaaatgg gggactgctt ttgcgatgga agaactagtc aacggtatca
    30181 aaaaaaatga agaagaagca cgcaaacagc ctcttcctaa acaacaaatg acattgcaag
    30241 aaatcgatag tatactgaca gaagcctttt tatctcgtaa agcagtaatc attcagctca
    30301 atttaaaaga tgaatttgga agactcttgg atgaggtcga agggcacttc attggtgaag
    30361 cctatgaaga ttactttatg attgaccatc agccgatatt gtgggaagac gtacggtata
    30421 ttgagttaaa ggaaacaaaa aaatggtttg tgacatgaga ggttgtgaca gaagtgatca
    30481 acttcaaaaa ataagaaagg atttttcgaa aattgcttct taaatttttg tggaattttg
    30541 gcttattttt cgaagaagca acttctggaa caacgtatat tagatatcag aggttgtgaa
    30601 aaagtcgctt agttttgagt aacaagccaa ggcgctgatt tcacaacctc tgtctatcta
    30661 tccatctatt cggtaagatt catgcatgac gtcttcttta tttagtatgt agagcccttt
    30721 ttcttctgac cattcaacat aaatcagctg ttttacatct gcatagcctt ctttcttcaa
    30781 gttctccata agccatgctt cagtcaattg attggactga agcactttat gctgaatttg
    30841 cccttcatct acaagcagga tcgataaagc tttgtcttca tctgattttt tcaatacact
    30901 catttgaccg gcattctcta ggataacatg tttggcattt tcaagaaaaa agcattcttg
    30961 ctgtcttaag attgctcgca actgctccat ctcgatatgg ttatttgtca actctgtcat
    31021 gttgatgacg ccgtctttga ttagaacaga tggctcgcct ttgacccaac gattgacctt
    31081 ttcatttttt tggacgatac gttcgatacc ataaatcatt actgcccaaa gcgcaatggc
    31141 aaataggaca tgccctacat ggacattgtc atcataggtt gattcttcta agatcccacc
    31201 taaaacaagc gtatagacta aatcaaacgg tgtgatttct gacatggatt tcttcccaag
    31261 caaacgtacc actaataata aaccgatcaa cccgaacacc agtttcattg taattgaaaa
    31321 aatcattttt tctcccccta actatctgtt aattaaatta tatatgcttt ctcacgaaca
    31381 cgcatggaat atacaaggaa gaaatgtatc cttttactaa aaaaaggact gtgacactgt
    31441 agtgaccccc aaaagttaga cttttttgga gtggagaaat ccactccttt tttttatgct
    31501 ctaaaatgat atttttacac aaccgttacg gttgttttac gaaattggac g
//
LOCUS       DAFEPQ010000024        31523 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.024, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000024
VERSION     DAFEPQ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.024"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(10..816)
                     /gene="proC"
                     /locus_tag="LP543_001268"
     CDS             complement(10..816)
                     /gene="proC"
                     /locus_tag="LP543_001268"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBL2321443.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            957..3530
                     /gene="mprF"
                     /locus_tag="LP543_001269"
     CDS             957..3530
                     /gene="mprF"
                     /locus_tag="LP543_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBL2321444.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            3672..4535
                     /locus_tag="LP543_001270"
     CDS             3672..4535
                     /locus_tag="LP543_001270"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBL2321445.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            4780..5718
                     /locus_tag="LP543_001271"
     CDS             4780..5718
                     /locus_tag="LP543_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBL2321446.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            5741..6421
                     /locus_tag="LP543_001272"
     CDS             5741..6421
                     /locus_tag="LP543_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL2321447.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            6394..7218
                     /locus_tag="LP543_001273"
     CDS             6394..7218
                     /locus_tag="LP543_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL2321448.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            7385..8203
                     /gene="purR"
                     /locus_tag="LP543_001274"
     CDS             7385..8203
                     /gene="purR"
                     /locus_tag="LP543_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBL2321449.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            8706..10079
                     /gene="glmU"
                     /locus_tag="LP543_001275"
     CDS             8706..10079
                     /gene="glmU"
                     /locus_tag="LP543_001275"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBL2321450.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            10173..11144
                     /locus_tag="LP543_001276"
     CDS             10173..11144
                     /locus_tag="LP543_001276"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBL2321451.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      11150..11252
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11318..11887
                     /gene="thiT"
                     /locus_tag="LP543_001277"
     CDS             11318..11887
                     /gene="thiT"
                     /locus_tag="LP543_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBL2321452.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(11968..12741)
                     /locus_tag="LP543_001278"
     CDS             complement(11968..12741)
                     /locus_tag="LP543_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321453.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            13007..14707
                     /locus_tag="LP543_001279"
     CDS             13007..14707
                     /locus_tag="LP543_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBL2321454.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            14677..14895
                     /locus_tag="LP543_001280"
     CDS             14677..14895
                     /locus_tag="LP543_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HBL2321455.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            14915..15805
                     /locus_tag="LP543_001281"
     CDS             14915..15805
                     /locus_tag="LP543_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBL2321456.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            15823..16107
                     /locus_tag="LP543_001282"
     CDS             15823..16107
                     /locus_tag="LP543_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321457.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            16594..17208
                     /gene="gmk"
                     /locus_tag="LP543_001283"
     CDS             16594..17208
                     /gene="gmk"
                     /locus_tag="LP543_001283"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBL2321458.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            17213..17518
                     /locus_tag="LP543_001284"
     CDS             17213..17518
                     /locus_tag="LP543_001284"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBL2321459.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            17771..20179
                     /gene="priA"
                     /locus_tag="LP543_001285"
     CDS             17771..20179
                     /gene="priA"
                     /locus_tag="LP543_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBL2321460.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            20194..20685
                     /gene="def"
                     /locus_tag="LP543_001286"
     CDS             20194..20685
                     /gene="def"
                     /locus_tag="LP543_001286"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBL2321461.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            20678..21616
                     /locus_tag="LP543_001287"
     CDS             20678..21616
                     /locus_tag="LP543_001287"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBL2321462.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            21616..22974
                     /gene="rsmB"
                     /locus_tag="LP543_001288"
     CDS             21616..22974
                     /gene="rsmB"
                     /locus_tag="LP543_001288"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBL2321463.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            22987..23727
                     /locus_tag="LP543_001289"
     CDS             22987..23727
                     /locus_tag="LP543_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBL2321464.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            23724..25793
                     /gene="pknB"
                     /locus_tag="LP543_001290"
     CDS             23724..25793
                     /gene="pknB"
                     /locus_tag="LP543_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBL2321465.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            25958..26857
                     /gene="rsgA"
                     /locus_tag="LP543_001291"
     CDS             25958..26857
                     /gene="rsgA"
                     /locus_tag="LP543_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBL2321466.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            26870..27520
                     /locus_tag="LP543_001292"
     CDS             26870..27520
                     /locus_tag="LP543_001292"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBL2321467.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            27521..28159
                     /locus_tag="LP543_001293"
     CDS             27521..28159
                     /locus_tag="LP543_001293"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBL2321468.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            28314..29168
                     /gene="mreC"
                     /locus_tag="LP543_001294"
     CDS             28314..29168
                     /gene="mreC"
                     /locus_tag="LP543_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBL2321469.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            29172..29681
                     /gene="mreD"
                     /locus_tag="LP543_001295"
     CDS             29172..29681
                     /gene="mreD"
                     /locus_tag="LP543_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBL2321470.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            29936..31474
                     /locus_tag="LP543_001296"
     CDS             29936..31474
                     /locus_tag="LP543_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBL2321471.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTVPESSATEE
                     SKTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVTPAPAP
                     SGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWTVPQES
                     AGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQWFAPDF
                     AVSM"
BASE COUNT        10343 a         5648 c         6798 g         8734 t
ORIGIN      
        1 ttgctatcat tattttttat tcaacatctc aagatctttc tcgatcgtcg tattgattcc
       61 tttgatgatt gctgggatga atcctgcttc ttctaaggca actacacctg caacagttgt
      121 accgccagga gaagaaactt ggtctacaag ttcccatgga tttttatcac ttgaaaggaa
      181 tttttgacaa gaacccataa ttgcttttgc agcaatctca gtggctgtct gtttggacaa
      241 accattcagt acaccagtcc gtcccatcgc atcgatcagc atgtaaacga atgcgggtga
      301 gctgccagca aggccaatga atgtactgaa atctgattct gctaaaggat agacgctgcc
      361 gattgcttca aaaatcatcg tagcttgctg aagctgttcc tccgtggcag cttcattttt
      421 gcaaatcgca gaaacgcctg ctcctacgct gacattgaca ttcggcatca cacgtacgat
      481 ggaggcctga ggatcttcaa gcgctgtttc taaatctgtt aatgattttc ctgaagcaat
      541 agaaatgaat aagactttct ggctatattc tcttagttct tccaatactt tttcaatgat
      601 ttttggtttc acggctaaaa gaacgatatc tgattgtgat gcaacttcct gattactttt
      661 gcatgcatgg acgccagttt cagcagcaaa tttttgtgca ttttcaaaat gtccactgga
      721 aacgaacacc tcttctttcg agactgttcc tttttttaat aaaccatcaa taatagcttg
      781 tgccatattc cctgtgccga taaatcctac tttcatgtta tcctctcctt tagcattagg
      841 atatctttct ttttctatca tcgcaagcaa agaacaggtc taaagtggaa aaattaaaat
      901 aaataagata acattggggt atgacacaaa agtatacaag ttaggaatgg gaaaatgtga
      961 aaaataaacg atacttgatg ctgcaatgga taaaggaaca tagtctgatt ttaaaactga
     1021 tttttttcgg ctccgtgtta gtctttgtgg caaatcaagt cgcaaatatt gctaatggca
     1081 tgagttggca agatatttgg caaaggatgg gacaacaaag ccgcatgacg atcctgctca
     1141 tgattctggc tggactgctg ggtgtcactc cgatgttgtt gtatgattgg gtgacgatca
     1201 gagtcttaga aaaacaagga aaaccaaaaa tggaaagaaa agagtttctt cttgctgcgt
     1261 gggtaacaaa cacgatcaac aatctcgccg gttttggtgg tgtggtagga gcaagtctgc
     1321 gggctagttt ttatggaaag cagacaaata gaaaaatggt tttagccact gtatcaaaag
     1381 tcgcactatt tatgctgact ggtttgtctg tatggtcgtt tcttacattt attgatgtct
     1441 tttttattca gtcagaaagt atttttcgaa gctattggat ctggcttttc ggcggtagtt
     1501 ttttagcacc agtcattttc ttttttgctt atctaaagag aaagacattg ttcttcgagt
     1561 tttatccaaa aggcttgctt ggtctgctac tggcatcctt tggacaatgg acgggagccc
     1621 ttgcggtatt tttgatcatc ggcaagttga tgagaataga tatttcgatg ctttcgatct
     1681 atcctatgtt catgattgct acattgatag gaatgctgac gatggtccca ggtggaatgg
     1741 ggacttttga cgttttgatg atcttaggaa tgagtcaatt gtctgttcgg caagatgtag
     1801 cagtagtttg gctattatat tatcggttgt tttattatgt tctacctttt atgacgggac
     1861 ttctattgtt catcacacat acaggagtca aaatcaatcg atttttagat aacattccgc
     1921 gactgttttt ccaaaaggtt gcgcatatcg tgctcgtaag tgctgtctat tttgcgggga
     1981 tcatgatggt acttctatca acaatcacta atctgtctgc aatcagtcgg ctatttcaag
     2041 tattattgcc gttctcattc aattttttag atcagacagt gaatctattg gttggctttt
     2101 tattactcgg ccttgctaga gcactttcag taaaagtcaa aaaggcattt ctgcctacga
     2161 ttgctttact atcttttggt attttaaata caatcacacg tacgttatct tggcagttga
     2221 tattggtata tgtcttgatt ctgttagctg tgtggttatc cagaaaagaa ttttatcgaa
     2281 caaaattcgt ttattcttgg ggagctataa tctttgatgc tagcttgttc ggctttttgt
     2341 ttatcgttta cgcaattgca ggctatcaca gcggacgctt tttagtcggt cgaataacag
     2401 gaaatcattt tattttgttt ccttcagatg atgtgtggtt ctcagggctt attggtttgg
     2461 gcatctcatt gctagcttta gtggcgcttt atcaatattt agctgaagat actacgaagc
     2521 ttggcagtga gtacgaaaaa gaccgttttg accgtttatt ggaaacatac ggaggcacag
     2581 ctacaagcca tcagcttcgc ttgtcaggtt acagttattt ttattaccaa gaagacgacc
     2641 aagatcaagt cgtttttggt tatcagatca aaggaaacaa atgctttgtt ttaggagatc
     2701 cagttggcaa tcctaaaatg atgcgggcag caacgaatca gtttatgaaa caagcagatt
     2761 gtttaggcta tcagcttgcg ttttataaga tatcagaaag ctacgtagtg atgcttcatg
     2821 aattgggttt tcacttcacg aaagtaggtg aagcagggat tgttgatcta gcaacagaag
     2881 acttgtctcg gctgagtcag caacttgaat atcaacgttt gatgaaagaa ggataccatt
     2941 ttacttggta cgatcaactg ccggaagaat tacatcaagc tgtaaatgaa gtttcacaaa
     3001 aatggcttca aggtgcacga gaaaaatact tctctgtggg acgatttgat aaatcatatg
     3061 tattatctag cggtgtcggt attgtccgta aagatcaaaa agtcgttggc ttcatcaccg
     3121 ctcagccgat gacgaagaag caggcgggat atgatttgtt gcgggtctta cctaaagaat
     3181 ctgaggaatt agctgactat ctgttagcca atctgtttat tgtatacgag aaaatgggct
     3241 actcggaagc caatctaggc ttagctccat tagctaatgt tggagaaaca gatttttctt
     3301 tctttcaaga aaaagtcatg catatcgtat ataattatgg taattttttc tattccttcc
     3361 agtctgctta tgaaaagaaa ttaaaatatg tcgatcattg ggaaggacgt tattttgctt
     3421 ataggaaagg aagtaatttc ggatttgcta ccatgcagct actcagtctg ataggaaaag
     3481 aaaaaggaaa tgttccgagt ttggcagaag aagtgctcac agaattataa gaatcactag
     3541 aacttgtttg gattatgaag agaaaattct gtgaaagctg ttgaaacgat gacaaaatca
     3601 accactaggc agacataagc tatactttta tttttcccta tgatagaata cttgcgactt
     3661 agacttttcg aatgggtgag aggatggaat tgattgaaaa agcgccagcc aagataaatt
     3721 taggactgga tgtattatat agacggcaag atgggtatca tgagttggaa atggtgatgt
     3781 cgagcgtaga cttggcagat catctatttt ttgaagaaat aacagaagac aaaatcattg
     3841 tagaaacaaa taaagcattt ttaccggtag atgaacggaa caatgtttat caagctgctt
     3901 tattgctaaa aaataaatac aatatcaaaa ccggtgtcaa gatctttgtt aagaagaata
     3961 ttccagtagc agctggatta ggcggaggaa gtacagactg tgcagctgct ctaagaggga
     4021 tcaaccaatt atggaatctg ggctgttcgt tagaagaact ggcagaaatc ggtttggaag
     4081 tcggtacaga tgttccttac tgtgtctacg gaagtacggc atttgtcggt ggcaaagggg
     4141 aagaaatcca agtattacct tctatgccac agtgctgggt tgtgttggtc aaaccaagaa
     4201 tgagtgtctc tacacgtaca gtgtttagag aagtagatac gcaagccatc caacatcaag
     4261 acatccacgg acttgctgaa gccatccgag agcaagatta tcagcagatg atcaaaaaaa
     4321 tgggaaacag tttagaagat ataacgggaa agcggcatcc agtcatccat caaatcaaag
     4381 agcgtatgat caagtacgga gcagatgctg cattgatgag tggaagcgga ccaaccgttt
     4441 ttgccttgtg tcaacattat tcaagagcgc agagagtata taatgggtta aaaggttttt
     4501 gtgaggaagt ttatttagta cgaaccctgc aataaaatat aagaagtaaa ataggtgatg
     4561 aaaaactgag gaatcctaaa atggtattct tcagtttttt attgtagtga aatggctaga
     4621 tatgagcagc aactcatcca ttttacgatt taatcaagaa tatacatgct taaatcagaa
     4681 attgatggat ttctattgac tgtgtttttt tattttgata cattagttca cgttaataaa
     4741 tcgtaaaatt ccgatttgtt cattgaaagg agcatataaa tgaaaaaata tcttttgaca
     4801 gctacggtaa tgataggtgc tttactgttt gcagcatgtg gaaatacgaa caaggaagca
     4861 gacaaaaaag aggatttgac gatcgttacg actttttatc cgatgtatga ttttacaaaa
     4921 gagatcgtag gggatgaagg aaacgtgaaa cttttgatcc ctgctggaac ggagccgcat
     4981 gatttcgaac caagtgcaaa agaaagagca gaaatttccg atgcagatgt tttcgtgtat
     5041 aatagttcag acatggaatt ttttgtggat tcgttaaagg attcagtgga tagcaaacag
     5101 accttgatga tcgaagcagc taaaggaatc gaccgtttag aatcgcaaga agcagacgaa
     5161 catgaagaat cagaagaagg gcatggtcat tcacatgaat acgatccaca tgtttggctc
     5221 gatccggtat tagctataaa agaagtcaga acgattgctg aagaattagg cgaaaaatat
     5281 ccagataaaa aggaaacttt tacgaagaat gccgatgctt atatcaaaaa gcttgaagca
     5341 ctggatcaaa aatactccga tgagctaaaa gatgcaacaa accgcacatt tgtcactcag
     5401 catgctgctt ttgcttattt agcgaatcaa tacgacttaa aacaagtagc gatttcgggc
     5461 gtttcaccag atcaggaacc cacaccaagt cgcttagctg agctaaaaga atttgtgaag
     5521 aaaaacaaca tcaaagtgat ttattttgaa gaaaatgcct cttcgaaggt agctgagaca
     5581 ttgtcgaatg aaacaggagt gaagctagaa gtgttgaatc ctttagaaag cttgactaat
     5641 gaacaaatca aagcaggcga aaattatatt tctgtcatgg aaaaaaatct gaaagcttta
     5701 aaagaaagta tcaattaagt tttagaatgg aggttgtaag gtgcgttata tcgaaatatc
     5761 tgatttgacc ttttactatg atgatgaacc tgtactagaa gatgtctctt atcatgtaga
     5821 tgcgggtgaa tttgtcattt taactggaga aaatggagca gccaagtcaa ccttggtgaa
     5881 agcatcgtta ggattactga aaccttcaaa aggaatgata aagattgctg agaaaaatca
     5941 agaaggaaaa aaattaagta tcggttatat tcctcaacaa gttgcatctt tcaatgcagg
     6001 ttttcctagt acggtgatcg aattagtccg ttcaggaaga tatccaagag gccgctggtt
     6061 caagcgtctc tctgaaaaag atcatcaaca tgtacagcgt gctttagaag ctgtagggat
     6121 gtgggatatg cgaaatcggc gaataggtga attgtctggc ggacaaaagc agcgaatcag
     6181 tttagcgcgt atttttgcta ctgaccctga cttatttgtt cttgatgaac caacaacagg
     6241 tatggatgaa gaatccagaa atgaatttta tcgtcttctc cgacatagtg cgcatgtcca
     6301 tgggaaatca gttctaatga tcacccatga tcatgaagat atcaaacagt atgcggacag
     6361 acagatccga ctggtgagaa aggaggattc tcaatggcgt tgctttcata tgaattcatg
     6421 agaagagcgt ttcttgccgc tatcttcatt gccggaatcg ccccgatgct aggtgtgttt
     6481 ttagtgatca gaagacaatc gctgatggct gatacgcttt ctcatgtctc tttagcaggg
     6541 gtagcgttag gattcttttt gaaccttaat cctacactta cgaccatgct agtcgttgta
     6601 ttagcagctg tgatattgga gtatctgcga acgatctacc gaacctattc agaaatctcc
     6661 attgccattt taatggctgg tggtttggcg ctagcgttag tattgatgaa tttgagcgga
     6721 gggaattcag caacaagtat ccaatcctat ctgtttggtt cgatcgtgac cattacatgg
     6781 gagcaagtca tctttttagg aatcttattc gttgccatcg gtatattgtt ctttttattc
     6841 aaacggccga tgtatgttct gacttttgat gaagataccg cccacgtgga tgggctaccc
     6901 gttcatttga tgtcgatgat gttcaatgtg attactggtg tggctattgc tgtgatgatc
     6961 ccgattgccg gtgcactttt gatatccgca atcatggttc ttcccgcagc aatcggtatg
     7021 cgattaggca aaagcttcaa tgtcgtgatt gctatcagtg tttgtgtagg atttctaggg
     7081 atgctgtcag gactaactag ctcgttttat ctagatacac ctccaggagc aacaatcaca
     7141 ttagtcttta ttgctttgtt cctgattgta aatatcatca aacgaatatt ggttgccttc
     7201 agaagaaaaa ataactaaat tataaagaaa agaagcctta taaaaacgaa aaatttttta
     7261 taggcttctt tttattttaa taaataccga actattttct tattttttca taaaatgctc
     7321 gaatttttga agaaaaactt atataatgta aaacgataaa atttcttaaa aggagttgga
     7381 ataggtgaaa gtacgtcgca gtgaacgtct agttgacatg acacagtatt tattgaatca
     7441 cccgcatgaa ttgatttcct taacaagctt tgccgaacgt tatgaatctg ctaaatcttc
     7501 tatcagtgaa gatttgggaa tcatcaagaa aacatttaaa gaacgagggt atggaacctt
     7561 ggaaacaatc ccgggtgctg caggtggtgt attgttcatc cctgaaattt catatgaaga
     7621 ggcgaaaaaa tatattgaat ctctagcaga acgtctatca gaacaagacc gtctgcttcc
     7681 aggaggatat gtttatctct ctgatttgtt aggagaacct gaactgctgc gtcaagttgg
     7741 aagaatcatt gcttctaaat accttggtga aaaaatcgat gctgtaatga ctgtagccac
     7801 aaaaggcgta cctattgccc aagctgtgtc ctattatctg aatgcgccat tcgttattgt
     7861 acgccgggac tcaaaaataa cagaaggatc aacagtaagt gtcaactatg tgtctggatc
     7921 ttctgaaaga attgaaaaga tggaactgtc taagagaagt ttgaaacgtg gttcacgtgt
     7981 ccttgtagtg gatgatttca tgaaaggcgg cggaacagtc aacggaatga agagcttgat
     8041 tgaagaattc gaatcagaac ttgttggcat cactgtattt gccgaatcaa aattcaacgg
     8101 tcatcgcgcc atcgatgatt atacgtcact gctttatgtc aaagatgtag atactcaaac
     8161 gaaaaatatt acggttgtgc caggaaatta tttttcagaa taagaggttg tgaaaaagcc
     8221 gcttagtttc gagtagtaag aaaaattttg tgaaaatggc tttccacatt ttttcataaa
     8281 tttggcttaa taccgagaaa ctggcttttg aacaccgttt atctagaggt tgtgaaatca
     8341 gcgtcctgtc ctgagtatta gctcaataaa cggaaaaatt gctcctcata ttttgccgtt
     8401 tattgagcta ataccgaagg tcagctgatt gaaaaacgtt taatgaagag gttgtaagcc
     8461 taccgtttaa ctctaagtca caagaaacga tttcaaaaaa cagcttttca tgttttatga
     8521 gtagtgtgac ttgtgaccga ggagttggca ggagagcacc ggttaccaaa tagataggcc
     8581 cgttatggtc tatctatttt tttatccaga aaacgctggg ataaaagaaa atcacttgtt
     8641 ttgttgaaaa aatgggtata ctgggataaa ctgaaatcag caaactatat ttaggagtga
     8701 cgcagttgga cgcacgttat gcaattattt tggcggcagg gaaaggtacc cgcatgaagt
     8761 caaaattata taaagtttta catcccgttt ctggtaaacc tatggtagaa catatcatta
     8821 atcgggtcag tgaaacgaaa ccagatgaag tgattacgat cgttggacac ggagcagaac
     8881 aagttaaagc acaactagga gaacgcagta aatatgcgtt acaagcagaa caattgggaa
     8941 caggacatgc tgttttgcaa gctgcttctt tcttggaagg aaaaaaagga acaacgttag
     9001 ttatcagtgg cgatacacca ttattgacta ctgaaacgct gaacaatctt tttgaatatc
     9061 accaaggaaa aaatgcaagt gcaacgatct tgacggcaca agcagaaaac ccaacaggat
     9121 atggacgaat catccgtgac catattggga tcgtagaaaa aatcgtggaa caaaaagatg
     9181 caacaccaga agaagctctt gtacaagaaa tcaatacagg aacttactgt tttgacaacg
     9241 aagctttgtt cgatgcgtta agcaaagttg gaacaaacaa tgctcaaggt gaatactatc
     9301 taacagatat catcgaaatc ttaaaagaag aaggccatac agttgctgct tatcaaacgg
     9361 atgattttga agaatcaatg ggagtcaatg atcggatcgc gttagctaaa gcaaatgaga
     9421 tcatgagaaa gcgaattaac cagatgcata tggtcaatgg cgtaagcttt gtcgattcag
     9481 caacgacata catcgatgca ggcgtagaaa tcggtccaga cacattgatc gaagcaggtg
     9541 tacaaatcca aggaaataca gtcattggtt ctgattgtgt catcgggtca cattcaaaaa
     9601 tcgtagacag taggattgag gatcacgtcg tcattgaaaa ctcagtcatt gaaagcagcc
     9661 atgtcaagaa acatgcagat gtgggacctt atgcacattt acgaccaaaa gctgaaattg
     9721 gcgaaaacgt ccatatcgga aactttgtgg aagtgaaaaa tgctcaaatc ggcaaaggaa
     9781 ctaaagtcgg tcacttgact tatgttggtg atgctacgct gggagaagaa atcaatgtcg
     9841 gatgtggtgt tgtcttcgtc aactatgatg gaaagaacaa acatcatacg actgtcggcg
     9901 accacagctt tattggttct agcacgaata tcatcggacc tgtcgaggta gctaaaaaca
     9961 gttcgattgc tgccgggtca acaattacag acaatattcc agaatatgcg ctggcaattg
    10021 cgagagcaag acaagtcaat aaagaaggat atgcaaaaaa actcccttat ctaaactaag
    10081 acttttgcaa aatcacttga tttatttaaa aaatatgact actatttatt aggtagaatt
    10141 ctagagttgt gaaaaagaaa gtggaggtcc ccatgtcaaa acattacttt gacccaagat
    10201 taaaaatttt tgcgttaaat tccaatcgcc cattagcaga gaaaattgca gcagcagtcg
    10261 gcgtcgagtt aggaaaatca tctgttactc aatttagtga tggagaaatt caagtaaaca
    10321 ttgaagaaag tattcgtgga tcgcatgtct atgttattca atcaacgagc agcccagtca
    10381 atgataactt gatggagttg ctgatcatga ttgacgcact gaaacgagca agtgcaaaaa
    10441 caatcaacgt agtcatgcct tattatggct atgcgcgtca agaccgtaaa gcacgtgcac
    10501 gggaaccaat cacagcgaaa ttagtagcaa acatgattca aaaagctggt gcaacaagaa
    10561 tgctgacttt ggaccttcat gcagtccaaa tccaaggttt ctttgatatt ccagtcgatc
    10621 atttgatggg tgcgccactt attgccaatt atttcttaga aaaaggaatc aaaggcgatg
    10681 atgttgtcgt tgtttcacca gaccatggtg gtgtcacacg ggcacgtaaa ttagcggaat
    10741 ttttgaaatc tccgattgcg atcatagaca aacgccgtcc aaaagcaaat gttgcagaag
    10801 taatgaatat cattggacaa gtcaaaggca aaacttgtgt actgatcgat gatatgatcg
    10861 atacagcagg tacgatcaca ttggctgcca atgctttgaa agaagctggt gcaactagtg
    10921 tgtatgcttc atgtacacat ccagtattat ccggccctgc tttacaaaga atagaagatt
    10981 cagctatcga gcgtctagtt gttacagact cgatctatct tccagaagat cgtaaaatcg
    11041 acaaaatcga tgaagtaagt gttggcggac taatgggaga tgcaatcaaa cgtatccatg
    11101 aaaacaaacc agttagtcca ttgtttgaaa caaaaaataa ataataaata aactcacaaa
    11161 ggggagtcca attgggctga gattgaatct atttctaaac ccttcgtacc tgtatcggtt
    11221 atgcgagcgt aggaattgtg aataaacagc tgtttgacta ttttcgaaag ctgcttcctc
    11281 ctttgtgact tttttattca aaggaggaag tttttttatg ggaaaaaata gagtttgggt
    11341 agaaggaacg attgttgcag cgttagcgat ggtgttgtcg ttgattccga tccagatcgg
    11401 cagcagtttt tctatttcat tagggcagat tccattgact ctttttgcgt taagaagagg
    11461 gacaaaggca gggatgctgg caggtttcat ttggggcatc ctacattttc cgttgggaca
    11521 agtttattat ttaagtgtcg ttcaagtgtt gattgagtac ccgattgcct atacttttgc
    11581 aggcgcagca gggctcatgg cttcaaatgt ccaaaaaggc tatgtagacg ataatacgaa
    11641 acaaattaga aaaagcatcg tgtttggtgc attgattggc gcactttcca gatatttttg
    11701 gcattttatt gcaggagtgg ttttctgggg agcgtatgca ctatggggga tgaattcttg
    11761 gctcttttct ttcgtaatga atggtgccag cgggttgagt acagctattg taacgatcat
    11821 cgttttatta gggatccaac aaacgactcc ttcgattttt gtgccagata aacgacagga
    11881 gctgtaaata aaaaagaagc aagaaacttg agacagaatc atgtcctagt ttcttgtttc
    11941 ctttttattt tttgataaga aatagatcta cttcaatggc agataagaaa gatcggcatc
    12001 ttctaccaat tgccagtgtc gatcggtgac agagtagatt aaagtagcga tgatttcctc
    12061 ttctcgatac cgggtgcctt ttaatttcca aatataatct tcaatcacat agattgggat
    12121 acgatattta ccatcgatca aaccaacttg atcaaaagaa aaaatgatcc cttcctgcat
    12181 caaattacgc atttctttca ataattccac actgattttt tcaacaggtg tttctttttt
    12241 gaaaaaagat ttttttacat tgatcttatc tgacaagatc gaacttaatt tactagcaaa
    12301 aatcctataa aattcatcta aatagcgata ataaatccga ttcagctgat cattaagatc
    12361 gaagtataca aaattattct gtagtttata aaaaaacggc gaatgaagat gtgttttcat
    12421 atgaccaaaa tataataatt ctgaaatttc taaaggtgtc aattctttca gcatcgttaa
    12481 atcagtaaag tcgatccact tcaattcatc gaatccacgc ctgttacgag aagaggaaaa
    12541 gaaacgctga atattttctg taccacggat tactttcagc cctgtatggg attcatattc
    12601 tccagcagaa gcagatggat caagcaagag caagttttga ggaaaatgca cgatactgtt
    12661 tgcaaagtca gtagcagtca gcccttttgt taaaacagca ttacttgtgg tatcaatatg
    12721 tacataaact gactcagtca tcatgttcct cctcttattt cctcttaatc tcttttattt
    12781 tacaacattt gcattaaaaa agcttgtttc ttttatttta aaattccatt acaaaatcac
    12841 gttttcattg aaaacactct cattttcaat taatttttgt gatttttgta ttttttattt
    12901 aaaggatgtt ttatgaattg ttttcttatg gaatctacaa agtcgaatac gagactcatg
    12961 acctttttat gacgtactgg tatacttgaa gaaaaaggag agcgcgatgg agataaaaaa
    13021 agtttacttt tcacagaaaa tgtcgctgta ttcgcctatc cagcaagcaa aacaacccct
    13081 tccatttttt caaagtcatc aagaaagtag agacgctttt tggcgttctg caaaaaagca
    13141 atgtgctttt aaagcagtat cgaaaaaata taccgtctat tttactattg ctaaaagtag
    13201 aggagatgtt atttttaata atctgttgat tgaaggtgaa acttttgaat ggaaaaaatt
    13261 ttttcggtat atggaagctt gcgctcgttt ttttattaaa gaaaactgtt gttttttatt
    13321 tcaatctcct ctatcagaag aatggttgcg tatttttcat aaaaacggct atcaaggaac
    13381 cacacaatta aataaaaagc tggtctatca tacagcactt gttttaggtg gaggaggagc
    13441 acacggtgct tatcagattg gtgtgtggca ggcgctgaag gaacatgata tcaactttga
    13501 gattatcaca ggtacctcgg taggtgcgtt gaacggcgca ttgattttac aaggagatat
    13561 gaaaaaagct gtgaatttat ggaaaaagct gtcgacaaga caagtgcttg cgttaccaga
    13621 aatggctgca gttgaagacc ttcgtgaacg cttctatcga gaaagacgcc aaatgacgaa
    13681 aacagcacta attgaaggcg gggtttcttc tgctccattg gaaaagatgg tcaaagacaa
    13741 tttggatttg tccttactca aacataatcc caaaatacgt ttatatacgg tgtctactaa
    13801 actgccagaa ctagcggaag tagtgactga tatccaacag acaaaaacag aagaaatacc
    13861 agattggata ctcgcctctg cttcatttta tcccgctatg gcttatcgaa agatcggaaa
    13921 aaataaatat gcagacggtg ggtatcgcaa caaaatacct attgatattg cgattaacaa
    13981 aggagcaaca gaggcatttg tcgtagatgt gcaagggcca ggcccagcga aaaaaattcg
    14041 tatgccagac atttttatcc attggaaatg tcaaacattg tggacactag gcagctttct
    14101 gctttttgat agccaaagga accaattaaa tttacaatta ggctatctgg aaatgaaaaa
    14161 acgattaggc tattactatg ggaactggta tacatttcat tctgttaaac ctgcaagaac
    14221 atactggcga ggatttttgt cttacttaac aaaagaaatt cagttagaat tatctttttt
    14281 caaaagcata aaattttggc aaaaactgcg taatttatat aagaacaggg tcgtaccaga
    14341 gacatgcgga ttggcgatgc tagaactttt agcaaaaaaa cattttttac tgcccaatga
    14401 aatctatcaa gtagacatga tgattcaaat gatctgccaa caagatataa aaagtatgcc
    14461 ggatgattta ttggcacaaa ttggtcaatt aagtactgaa gaatggcgaa ggtatcgaaa
    14521 ataccagcaa aaaatacaaa atgagcgcac gaaaactgtt tattttgatt atctgctaca
    14581 aggaaaacgc aaggatcgtt tgcagcatga attgcttaag caaccagttg acacattact
    14641 gatcctatat ttatattatc tcaaggagga acaaccatgg cacaagaatt ttcatatgaa
    14701 atcgtagaag aaatcgctat attgtcagaa aacaacaagg ggtggcgtaa agaactgaac
    14761 ctagtcagct ggaatggtcg cccgccaaaa ttcgatcttc gagattgggc gccagaccat
    14821 gaaaaaatgg ggaaaggcct gacattgacg aacgaagagt ttgaacaact gcaaaaagca
    14881 atagaaaata tgtaatgagg aaggaccctg tcgaatgaaa agtatgactg gttttggcaa
    14941 agccatccgc gaaacaaaag cgtatcagtt agatattgaa ataaaaagtg tcaaccagcg
    15001 ttttctagat gttcaaatac gaagtcctaa aatcctgaac tttgttgaaa acgattttcg
    15061 tcagcttatt aaacagcagt taagccgcgg tcgaatcgaa gtatttgtga atctagctta
    15121 tattggcaaa aacgaaaaac agatcgtcat caactgggaa ttgattgatt cacttatcac
    15181 tcaattgaca gacggaattc aacaacgata cggtagtcaa aatcaagtgt caattagtcg
    15241 gatggcggaa cgcttcgcgc tggacgaaaa cttcgtaacg gtagaagaaa aaccggaaga
    15301 gagcatggaa gaattggcac agttagctct agatacattg aaaagcgcac ttgattctat
    15361 tgaggcgagt cgagaaaaag aaggtcaagc tttagcttta gtgttgaaaa agaatactga
    15421 agaattcaaa gaagtactaa aaaaactcga tagttttgta gaaatctatg aaaaggaatt
    15481 tcaaataagg tatcaaaaaa agctggagga atttcttgga gcgaaagtcg atgagcaacg
    15541 gttgttgacg gagctagcca tcttgttgga acgcggagat attcacgagg aactggatcg
    15601 tctggcgatc catgtacaaa aactagatga acttatcgaa gcacaatgtc ctgtaggtcg
    15661 ggaattagat ttcctgatcc aagaaatgaa tcgggaaatc aacacgatcg gttccaaatc
    15721 aaatgcaatc gaaattaaaa atcaagtcgt ccagtctaag acgatcttag aaaaaatcag
    15781 agaacaagta caaaatattg aatgacgaaa gaggtagaga taatgaaata ttatgacgta
    15841 acgttccacg aattaagtgg aaaaactgtg gttaaacggg atatcccttc tgaaaaaaac
    15901 ggctttgacg tatggaaaga tgcctgtgca gactataatg agaacgaact gtttattttg
    15961 atcaacgacg gtgcttacgt gacgatgaat cggaaattta ttgttcgaat tgacacagaa
    16021 gaggtagaag atccgacaga aaaagcacgt tctcgaaaag atgagatcat gggtgtagtg
    16081 aatactttgt ccaatatggg cttttagcaa ggatgctgaa caaagaaata caataaataa
    16141 tcagaattgg atttcttcgc ttagcttctt tgctctagga atacatttaa aggttgtgac
    16201 aaaattcgtg tcacaatctt tttttatttt gaataagtga tgttcaaaaa ccattttctc
    16261 ggtattaagc caaatttatg caaaaatgtg agaaactatt ttcacaaaat ttttcttcat
    16321 actcgaaact aatcggcttc ttcacgacct cttatacacg gcgttcaaaa gcttgcatct
    16381 gcgataataa ccccaaccaa acgaaaaaca tgagaagctg tttatgtttg gctgttctta
    16441 ttacttgatg caggacagct tttttacgac ctcttggtac agggtagctt ttttaccaat
    16501 ttctagctat aattaaagat aaccttgaaa gttgcagaaa aaaagtgcaa aataaacgat
    16561 aagtacaaaa agactagttg aaaggacgtc gtcatgtcag agcgtggatt attaatcgtg
    16621 ttgtctggtc catccggtgt aggaaaaggc acagtacgaa aagcaatttt tgagagtgag
    16681 gataatgatt ttcagtattc gatctcaatg actacccgta aaatgcgtga aggggaggtc
    16741 gaaggcgttg attatttctt ccgaagcaaa gaagagtttg aatcaatgat cgaagctgga
    16801 gagatgctgg aatacgcaga atatgtcggg aattattatg gaactccttt gtcatatgtc
    16861 aacaagacat tggatgaagg aaaagatgtt ttcttggaga ttgaagtcca aggagcaaaa
    16921 caagtcaaag aaaaagtgcc cgatggtgta ttcatctttt tgactccgcc agatttagcg
    16981 gaattaaaat cacggatcgt aggccgagga acagatgcag atgaagtaat cgaagaacga
    17041 atgaaagttg caaaagaaga aatcgaaatg atggctcttt acgactatgc tgttgtaaat
    17101 gatgaagtgc cgctagcagt tgaacgaatc aaaaacatta ttgccagtga acattttcga
    17161 gtagatcgcg taatcggtaa atatattaaa atgttaaagg agatgtagtc ttatgatgtt
    17221 aaaaccttca attgactcac tattagatcg tgtaaattca aaatattctc tagtgatttt
    17281 agcaagtaaa cgcgcgcatg aattggatgc aagtgcacag cctactctag attcttttga
    17341 ttcagtaaaa agcgtcggtc aagctttaga agaaatcgat gcaggaaatg tagtcaatga
    17401 tccacatcct gaattaaaac gtgaacgttt gaagatggaa gaagaggaac gtcaagcaca
    17461 aaaagagcgt gaacaacacg aattggaatc acgtatccgt gaagagcaaa aaatgtaata
    17521 aagaaaaata gagcgaacat ctaaaaagaa aagcaaaccc agctgtttcc ttttcaaaca
    17581 ttcgccactt attgtctggt agaaaaggct gtgtctgaac tttttgacac agttcttttt
    17641 tttcttatct gctgttaatt ttttgttagg atttggcaga agataagaca aatccatcga
    17701 tctctagtaa aataggcaag gaactttttg tcgcttgata agaaaagaca aaaaaaggag
    17761 gaaacgaacc atgacattaa cagctgaagt cattgtggac gtacctacta tgcagaccga
    17821 tcagccattc acttatttgg ttccctctga aatggaaaca gctttgcaag tcgggatgcg
    17881 agtagaagtc ccttttggta atggcaatcg tcatgtccaa ggatttgtta tggctatcca
    17941 aaaatcagaa gaatcagcta atccatcttt gaaggcagtt attcgtttgc tagaccttgc
    18001 tccggtggtc aatgaagaat tactttcatt agctgattat atgaaaaaaa ttacttacgc
    18061 attcaaaatc acttgtctac aaacgatgct tccgagtgtg atgaaagcag aatatgataa
    18121 attgatctat cctttggcag acacaccaga agttcaggta ttgtttgatc agcgagaagt
    18181 gatttcctgg aaggaagcag aagaagaagg cagcctttct caattgattc gctggcgcca
    18241 agagcaatta gtagatatca aatatgaagt ccatacacga aataaagtaa aaacgattcg
    18301 tttggtccgt tctttgttaa ctgaaaagca aatcgaagaa gaatgggcaa aattacggca
    18361 aaatgccaag aaacaaaaag aacttctttt gtgtttatct gaactgagcc aagaagaacc
    18421 aattgcttat ttcaaaaata aaggaatcag tactgctgtc ttgaatcaag gaaaagaaaa
    18481 gggctggctg gagtttatag aatcagaacg ttatcgcgac ccttataaag accgagtatt
    18541 tgaccaaacg acggcattgg aactgaatgc agaacaaaaa aatgctgtcg aacaaatcat
    18601 cacagcaggt cagcagcaaa aagatcaggt ctttttgctg gagggaatca ctggcagcgg
    18661 gaaaacagaa gtatatcttc aggcaatcgc tgatgttttg tcagaaaata aaacggctat
    18721 tatgttagtg cctgaaatcg cattgactcc gcaaatggtc gagcgattca aaggacgctt
    18781 cggagaatca gttgcggtct tacatagcgg actttcccaa ggtgaaaaat atgatgaatg
    18841 gcgcaagatt gagcgggaag aagcgcaagt agtagtaggc gcacgatctg ctatttttgc
    18901 cccgctgaaa aatatcgggc tgatcattat cgatgaagaa catgaatcaa gttacaaaca
    18961 agatgagaca ccgcgttatc atgcgagaga tctagcaatt tggcgcagta aatatcatca
    19021 ttgcccaata gttctaggaa gcgcgacgcc ctctttagaa tcaagagcac gagctcaaaa
    19081 aggcgtttat cagttattgc agttaaacca ccgagccaaa gcagctgcac agcttccagc
    19141 tattgagatc gtagatatgc gtgaagagtt tcagaatcat cggacctcta ctttttctgc
    19201 taatttgcag gaaaaaatac aaaatcgatt agacaaaaaa gaacaaactg tcctactgct
    19261 caatcgtcga ggatattcgt cttttgtgat gtgtagggac tgtggatttg ttcttccatg
    19321 tcctaattgc gatatctctc ttacactgca tatggataca cgttcaatgc gctgccatta
    19381 ttgcggacat gaagaaccta ttcccaatcg ctgtcctaat tgtggcggca ataaaatccg
    19441 ctactatgga acaggtactc aaaaagtaga agaggaactc cgagaacttt atccgcaagc
    19501 taggattttg cgtatggatg tagataccac tcgaagaaaa ggggcacacg aacagatttt
    19561 acaaaaattc ggtgcaaaag aagcagatat attattaggg acacaaatga ttgctaaagg
    19621 tttggacttt ccagaagtga cattagttgg ggtgttgaat gctgatacct ccctgaattt
    19681 accggatttt cgttctagtg agcatacatt ccaattgctt acccaagttt caggacgagc
    19741 agggcgagcg gaaaaagcag gcgaagtcgt gatccaaaca ttcaatcctc aacattacgc
    19801 catagaattg gctaaaaaac aaaattatga gcagttttat cagcaggaaa tgcatgtacg
    19861 gcatcgtgga ggctatcctc cttattactt tactgtgaaa ataaccgcca gccatcctga
    19921 agaacaagta gcggcaaaga aaatctttca gattgccaat cagctaaaag aagtgttaag
    19981 ccctcaaagc ttgctattag ggccaacgcc cagcatgatt ttgagagtga aaaaccgata
    20041 ttattatcaa ctaatcatca aatataaaca tgaaccgaat cttcctcatg ttttggatga
    20101 aattttgaat ggaagccaga aagaacagcg acaaggtctt tttgtggcaa ttgacaacga
    20161 accattaaac tttatttaag aaagggccaa attatgcgct atcctatatt gatccaccct
    20221 aatgataaat taaaaagaac tgctcagcca atcgatgtaa tcacggacga gacaatcgct
    20281 ttattagaca atttatatga gacaatgatt gcgaatgacg gaatagggat cgctgctcct
    20341 caagtaggac aaaacaagag aattgctgtg atcgaagtag atgaaggcga gaaatttgaa
    20401 ctgattaacc cagaaatcat cgaagcaaaa ggtgaaagcc ttgacgtaga aggttgtctc
    20461 agtattccgc atgtctatgg aacggtcaag cgagcagatg aagtgaccgt gcgttattat
    20521 gatcgagacg gagaagagat agaagtgacg gcttttggct atttagcccg tgccttccag
    20581 catgaaatcg atcacttaga cggtatcttg ttcattgaga aaatgataca gcagatcccg
    20641 gaagaagaac ttgaagaata tatggaggaa catcttgatg actaagctag tatttatggg
    20701 cacacctgct ttttctgtgc cgattcttga agggctgata gaatcaggtt acgagatcca
    20761 ggcagtggta acacagcccg accgtccagt tgggcggaaa cgagtaatta cgccgacacc
    20821 ggtaaaagaa gcggctctga agcacggtat ccgtgtgctg cagccagaga aaatctccgg
    20881 ttcgccagaa atggaagaaa tcattgaatt ggtgcctgat gtgatcgtca ctgcggcatt
    20941 tggccaattc ttgcctgaaa agttattgca agtaccaaaa ttaggggcaa tcaatgtcca
    21001 tgcttcttta cttcctaaat atcggggagg tgcgcctgtt cattatgcca tcatgaatgg
    21061 ggagaaagaa actggtgtaa cgatcatgga aatgatcaaa aaaatggatg caggcggaat
    21121 ctatgcgcaa gaaagcatac caatcactaa acaagatgat gtagggacga tgtttgaaaa
    21181 gcttagcctt ttaggaagaa agctattgct agaaacattg cccaatattt tggatggaca
    21241 aaaaccagta cctcaagacg agtcagaagc aactttttcg ccaaacatca cacgggaaca
    21301 agaagcaatc gattggcata aaactgcaga agaaattgac aaccaagtac gcggaatgcg
    21361 tccttggcca attgcattca cgacctatga gggcatacgc tggaaattat tatcggttga
    21421 agtattggca gaaactacag acaaagcacc tggtacgatc atccggaaag acaaaaaaaa
    21481 tctatggatt gcttgcggta aacagaccgt cttagccatc aatgaattac agccagctgg
    21541 taaaggaaaa caggccgtac atgatttcct taacggaagc ggtcagcaag ttcagattgg
    21601 tcagcaggtg aaataatgaa aaaaatccct aaaaaaatga aaaaatcagt acgatatatg
    21661 gcattactga cattagagcg agtgaacaag ggtggcgcct actcgaatct tttgttgaat
    21721 gagatgatca aacaaagcga gctaaatcaa aaagatgtgg gtttatttac agaattgatc
    21781 tatggtacga ttagccatca gcgactattg gaattttacg ttgcaccatt gatcgctaaa
    21841 gcaaaaaaag tggatgactg ggtcaagacg cttctttatc tttctgtata ccaattagaa
    21901 tttttagata aagtccctgc acatgctatt ttaaacgagg cggtcgaaat cgcaaaagta
    21961 aaaggaaatc ctgggacagg gaaatttgta aatggtgtct tgaggaatta tcagcgtcaa
    22021 ggagcaccag atctgcaagg cattgctgat cctatcgaaa gactttctgt caaaatcagc
    22081 ctgcctctat ggatgactaa acgactggtg gatcaaatcg gatacgaaga aactgaaaag
    22141 ctcggtttat cgctatatca gcctagccat gctagtgccc gagtagatac acgccgactt
    22201 tccagagatc aggccatcac tgttttgcaa gaagaagaga tcacagctga aaaaagccaa
    22261 gtcagtccat acggtatagt agcgaaaaaa gggcatttag cttctagctc attgttccac
    22321 gatggcgtca tgacgatcca agatgaaagt tcgatgcttg tagcgccgtc tatgcaaatc
    22381 gagcctgaac atcatgtctt agatgcctgt gcagcacccg gaggtaagac gacgcatatt
    22441 gcttcttttt tagatccgtc atgcggtgga gaagtggtcg ctttagatgt acacgaacac
    22501 aaagtcaaac tcattgaaga taatgcagca cgtctaggtg tctcttctgc tgtctacgct
    22561 caaaagatgg atgctagaga agtcagtaaa cagttcgaag cagagcagtt tgatcggatt
    22621 ttagtagacg cgccttgttc aggtcttggg ctgcttcggc gaaaaccaga tattcgttat
    22681 aaaaaacagc cggatgaatt agcgaattta ccaaaaatcc aattggaaat tttagaaagc
    22741 tgcgcaccaa ctttaaagtc ttccggtatt ctgacttata gcacttgtac gatcttaaaa
    22801 gaagaaaacc aagacgttgt ttctgctttc ttggaaagac accctgattt tgagaggatt
    22861 gatgttttga ccaatcctat gctacaatca tcaatcgaag agaaaatgtt gactttgtat
    22921 ccgcatcagt tttacacaga tggatttttc atttgttgtt tgcgcaaaaa gtaatgaggt
    22981 gaaaaaatgc agattgaata tcaatcagat gtaggaagac gacgtaacac aaatcaagac
    23041 tatgccagtg tatttaccag ccgttcaggt ctcaagcttg ctgtattagc agacgggatg
    23101 ggtggtcatc gtgccggaga cattgccagt cagatggcgg taacgaatct aggcgaagct
    23161 tgggaaaaac aagaagctgt tgacgatgaa aaaatcgccc aatggttcat tcaatcgatt
    23221 caagaagaaa atatgctgat ttatcagcgt ggacaagaac agccggaata taacggtatg
    23281 ggaacaacaa tcgtagcggc cgctctgtca gatgactggt tcacgattgc caatgttggg
    23341 gatagcagag cttatttgat tcgtgaagat aaaatcatgc aattgacgga ggatcattct
    23401 ttagtcaatg aactagtcaa atcaggagaa atcagcgaag aaatggcagt gaaccatcca
    23461 aggaaaaata ttttaacacg ttctgtcggc atgcctggaa cagtcgaagt agatgtttct
    23521 actcatcttt ggcaactaaa agatcgactg ctgttatgtt cagatggctt gacgaacatg
    23581 ctgtctgaag aagaaatagg aacgatcatc aatcaagaag gcccattgtc tcataaagta
    23641 actgagctga ttgatcgtgc caatgaagca ggcggagcgg ataatatcac cgttcttctt
    23701 atcgaatata aggaggacag tgtatgatcg agttgggcaa aaaattaaat ggccgttatc
    23761 aaatcactgg aaatatcggt agcggtggta tggcaaatgt atttttggcg cacgatttaa
    23821 tcttggatcg tgatgtagca gttaaagtct tacgatttga ctttcaaaat gaccagactg
    23881 cgattcgacg atttcagaga gaagctttag cagcaacaga actcgttcat ccaaacattg
    23941 ttagtgttta cgatgttggt gaagaagaca acatgcagta tctggtgatg gaatacgtca
    24001 aaggaatgga cttgaaacga tatatccaaa cacattatcc tgttccttat gaaacagcag
    24061 taaatattat gcagcagatc ctttctgcta tttctttagc tcataaccat caaatcatcc
    24121 accgagattt gaaaccccaa aacgtactga tcgataatga aggtgttgta aaaatcacgg
    24181 atttcgggat tgctatcgct ttatctgaaa catcgatcac gcagacgaat acgatgctag
    24241 gatctgttca ttatctgtcc cctgagcagg ccagaggaag tatggcaacg aagcagtcag
    24301 atatttatgc tttagggatc atcctttatg agatgttgac gggtagcgta ccgtttgatg
    24361 gtgaatctgc tgtaacaatt gctttaaaac attttcaaga tgatcttcca tctataaaag
    24421 cacttgatcc aaatgtgcca caagcactgg aaaacgtgat tttacgggca acggctaaag
    24481 aacctgcaga ccgttataag tcagccgagg aaatgtctga tgatctttct acagtattat
    24541 caccagctcg tgcaaacgaa gaaaaatggc agcctcatgt gatggataat gaaacaaaag
    24601 ttatcacacc ccttacggaa gatgcaccaa tgcctgattc attcaaatct atgcctctgc
    24661 caaaagacaa gccagaaaac gaaaccttac cagaggagga agaagttctg cctgatccta
    24721 aaaagaaagg acagaaaaaa tggtggctga tcctattagc tattcttgct gttttaggaa
    24781 tcggaacagc tgtattcttt gcttctggcg ggcgaggaga agttgttgtt cctgatgttt
    24841 ctgatctttc tgaagcttct gctagagaaa cactcgcaaa agcgaaatta gaagtagcaa
    24901 caaaaacaga agaaattgca gatgacaaaa tcgaagaagg taatgtagtg aagacagacc
    24961 cggcagcggg aacgacagtc aagcaaaaac gcgaagtcac tctgtatatt agtagcggca
    25021 cgaaaaaaat caaattagat gattacagtg gtaaaagtta tgaagatgcc gaagcaagtt
    25081 tgaaagaatt agggttttct gctgacttga ttcaaaaaga agaggaattc agttcagagg
    25141 tagaaagcgg aatgatcatt tcgcaatctc catcagaaga tacagaagtc gatcctaaaa
    25201 cagacacgat tactttcaca gtcagcaaag gaccagaaac agtgactttg agtagtcatg
    25261 taggagaaaa ctacgaaact gttcgtaatt ggcttgttac acaaggattc aatatcaata
    25321 atattagtgc tacttatgac tatagcgaca ctgtaccatc aggttcgatc atcagtcagg
    25381 atccagcctc gggtgaagtt gtagcagaag aaacttacat caattttgtc gtaagccaag
    25441 gaacagagcc taaattgaat gatatctccg gctatacaaa aagcgaagca caaagttatt
    25501 tagctagtgt cggtgcagaa tatatcgggc atgagacttc cgaatattca aacacggtgg
    25561 ataaagataa agtgattcgt acagtaccag gggcaggaac gacaattacc aaaggaatgg
    25621 ttgtgaacgt aatttactct aaaggaccag atccagcgtc ttctagttct gaaaaagact
    25681 cttcagacag ttctagcagc tcaagtagct ccagtagcac aagcaactct tcaaaagaag
    25741 aatcttccag ctccacaagt tcatcagcta gcacctcaga aagttcaaaa taagtgctga
    25801 ataaaatata tcagaaggga atgcgacatt gttttgttgc attctctttt ttgtgtttct
    25861 atctcttttg atcaattttc aagccatata ctttgagaag tttgcatgga ataacaccta
    25921 ctttcctctt cgtatggtaa aataaacaaa aacggaggtg gcacttatca aaggacaaat
    25981 cagaaaagca atcagcggtt tttactatgt atacgcaaac ggagagacct atcaaacaag
    26041 aggaagagga aatttccgga atcggaaaat cacgccgctt gtcggagatc aggtgatctt
    26101 tgagagtgaa aatcaaacag acggctatct tctggaaatc ttgccgagaa aaaatcaatt
    26161 agttcgccca ccagtcacaa atgttgatct tggaatcatt gtcacaagta tcgtagagcc
    26221 tgcattttct tataatctac ttgaccgctt tctagttacc ttggaatacg agtcgatcga
    26281 accgattatt tttttgacaa agaccgactt gtcagacgac ccagaaaaaa tcgaagagat
    26341 caagcaaacc tatactaaaa taggctatcc ggtgattgta cccaatcata cgggtgatac
    26401 gacagaactg atccgttact tcccagaacg gctatcggtc tttatgggtc agtctggagc
    26461 aggaaaatcg acgctactga atcaaatatc accggaactg aatttagcaa caggagagat
    26521 ttctgatgca cttggaagag gacggcatac aacacgtcat gtggaactga ttcctatcta
    26581 tggcgggtta gtagcagata caccaggatt tagctcgatt gactttttga caattgaagc
    26641 aggagaactg ccgagacaat ttcctgaatt tgtagaagct tctgtccatt gcaaatttag
    26701 agagtgcatg catgctaaag aacctggctg cgaagtaaaa catagagtag aaacaggtgg
    26761 tatcgcacag acacgttacg acaattattt gcaattctta caagaaatcg aaaaaagaaa
    26821 accaatgtat gcaaaaaaag aaggtaaacg aaaataggag gaaacaagaa tgaagatagc
    26881 accatccatt ttgagtgcag attttgccaa tttacaaaga gacattgagt tagtggaaaa
    26941 aggaggcgca gattacatcc acgtagatgt aatggatggc caatttgtcc caaatatcac
    27001 attagggcca aatattgttc aggcaatccg tcctgttact aaattaccgt tagacgtcca
    27061 tctgatgatc gttgatccag aaaactacat tccagcattt gcaaaagctg gagcagatat
    27121 cattactgtc cacgtcgaag caactcctca tattcatcgg gcacttcaaa tgacaaaaag
    27181 cttaggagtt aaagctggag tagttatcaa tccaggaaca ccagtctcca tgatcaaaca
    27241 tgttttgcct ttggctgatc aagtactagt catgactgtc aatcctgggt tcggcggaca
    27301 aagctttatt gaagaaacag tagaaaaaat tacggaactc gctgatctac gtagagagaa
    27361 caattggact tatgcaatcg aagtagatgg cggaatcgtt ccagaaacag caaaaatctg
    27421 taaagatgca ggggcggatg tcttcgttgc agggtcatat atctatggag ccgaaaatcc
    27481 agtcggacaa atcgaacgat taaaagaggc attagactaa atgaaaattt tattagctgc
    27541 tggaggtaat ccgcaaaact ggccgcagtc tctgctgtcc aaatacgatg cctatgtcgg
    27601 gatagaccgt ggtgcgctgt acctgcttca agcaggctac ccagtagatc tggcagtggg
    27661 tgattttgat tcattgtcga acgaagagca gaaggaagta tttgcaaaag ccaaagagta
    27721 tacccgttcc cctgcagaaa aagacgatac agatacacag ttaggtatct taaaaacatt
    27781 tgaacgttac ccgcaagcgg aagtcacttt gattggagct acgggcggaa gattggatca
    27841 tcttttagcc aatctctggt taggtatgga acctcgcttc aaaccctttg cccatcagct
    27901 tattatcaga gattgtcaaa attatttgtc ttattatttg cctggagagc atatagtaag
    27961 acagatacca gatatgaagt atttagctta ttgctgcttg actcccgtct cgaatcttac
    28021 attaaaaggc agcaagtatg tattggaaaa taaagaagta gaatatccga catcttatgc
    28081 aagtaacgaa tttctttcag ataccgcaga attttctttt tcagatggta tgattgcagt
    28141 tattcaaagc aaagattgat ccataaacaa ataaaaaaat ttgtctttca ggctaaaaat
    28201 gacagaatct attatttgct aaaaaaaagc ttaagaggag caacttacct tttgtttcac
    28261 ggcatttagt gataaaattg aacacaagag tctaattaat tcgaggtgtc tttgtgaaga
    28321 agttcaatcc taataaaaat attattatta ccttggttat tgttatcatc gtggtgacga
    28381 tactaagtgt gaccgctgcg cagcgagcga atgaaggaaa gcccaatttc ttccaatcca
    28441 tcgttaatga cagtgttgca atggttgata aaacaatctc tgccccagta cggtgggtac
    28501 gaaatggagt agattctgtc cataatcttt tttcaactta tgccgaaaat gagcggctaa
    28561 aagaaaaaat cgatagctat gatgacgttt cacagcaaaa caagaattac gagagagaaa
    28621 tcgaagcgtt gaaaaaagaa cttgagttga atcagacgct gactagttac gaaaaagtaa
    28681 cggcaaatgt cattactcgt tcgcctgaca gttggcagga catgctgatc gtagacaagg
    28741 gttctaaaga cggtatcgaa gcaaatatgg cagtcatgtc gcaaaaagga ttgattggcc
    28801 gagtgatcga ggtcaatacg gcttcgtcta aaatcgaatt actgtcatcc tctaatgaaa
    28861 gctccaatca ttttccagta cgggtatctt cggctaatgg cgaagcgttt ggtttgctta
    28921 aaaactatga tgaaaagctc catgccttag tggtgaccca attaactggt gatacggata
    28981 tcaaagaagg ggatgttgtc cagacatccg gtcttggagg gaattctcca gctaacttgc
    29041 cgatcggtac ggttattaaa acgaaaccag atagttatgg gctggatcgg gaagtttatg
    29101 tgaaacctta tgcagaaatg tatgacgtgt cagttgtgac gattgtccaa cggctagcgg
    29161 aggattagtc catgttgaag aaagaaacaa tgaagtacta tctgccaatc gttttgttct
    29221 ttttgatgtt gatagatggt catttaacaa gaatgctagg ggagtggtcg aaaggcacct
    29281 atatgtcaaa tgcccacttt ctgatattgg cattattatg ttgcagtatg gcgtttgaaa
    29341 aacgttattt actgattacc acgattgttc tcggggctat ctatgacgct tactatattg
    29401 gcgttatcgg tatctatgca gtagctctcc ctttaattgt atggttgatg tatgtaatga
    29461 aagacgttat ccatgtcaac atctttactg aatttttcag tatgatcatc tttgtcacgg
    29521 gttatgaatt gtttacgatg gtggtccagt tgatttttaa attagcagta gtaaataaca
    29581 cgtattttat tacaaggttt ttaggaccta cactgctgtt gaacatgatt atatttgtat
    29641 tattcatttt tccctttaag aaattattca gtgatgaata atttcttttt ttatttttag
    29701 gtcgaaagcg aaaaagaatc gttgttatct taagaaatgc tagacttaac gcggcttttt
    29761 catgatcatt ttctgaaacg aacagttttg tcatttttaa aagttgtttt gaaacatttt
    29821 tgtaacataa ctattatatc gttgacacac tgctatggta caatttttat atcgttaaga
    29881 aagacttaaa tatatttaga tatttattaa attaagccag tcggaggaat gaagagtgaa
    29941 aaagagttta atatcagcag taatggtatg ttcaatgacc ttgactgccg tagcatcacc
    30001 gatcgctgcc gcggcggacg attttgattc tcagatacaa cagcaagatc aaaagattgc
    30061 agacttgaaa aatcaacaag cagatgcaca atcacaaatc gatgccttag aaagccaagt
    30121 ttctgaaatc aacacacaag cacaagacct attagcaaaa caagatacat tgcgtcaaga
    30181 atctgcacaa ttagtaaaag acattgcaga tttacaagaa cgtattgaaa aacgtgaaga
    30241 tacaatccaa aaacaagcac gcgaagcaca agtaagcaac acaagctcaa actacatcga
    30301 tgctgtattg aatgctgatt cattagcaga tgctattgga cgcgttcaag caatgacaac
    30361 gatggtaaaa gcaaacaacg acttgatgga acaacaaaaa caagataaaa aagctgtaga
    30421 agacaaaaaa gctgaaaacg atgctaaact aaaagaatta gctgaaaacc aagctgcttt
    30481 ggaatctcaa aaaggcgatc ttttatctaa acaagctgat ttgaacgtac tgaaaacttc
    30541 attagctgct gaacaagcaa ctgctgaaga caagaaagct gacttgaacc gtcaaaaagc
    30601 agaagctgaa gcagagcaag ctcgtattcg tgaacaacaa cgtctagcag aacaagctcg
    30661 tcaacaagct gcacaagaaa aagctgaaaa agaagcacgc gaacaagcag aggctgaagc
    30721 acaagctact caagcttcat ctactgcaca aagctcagca acagaagaat catcagctac
    30781 acaatcttca atgactgaag aatcatcatc agctacacaa tcttcagcaa cagaagaatc
    30841 aacaacacct gaatcttcaa cagaagaatc aacagcgcct gaatcttcag caacagaaga
    30901 atcaacaaca gtgcctgaat cttcagcaac agaagaatca aaaacagtgc ctgaatcttc
    30961 aacaacagaa gaatcaacaa cacctgcgcc tacaacacca tcaacagatc aaagtgttga
    31021 tacaggaaac ggcacaggaa gtagtactcc ggctccaacg ccaacaccaa cacctgaaca
    31081 accaaaacct gtaacacctg ctccagctcc ttctggaagt gtgaatggtg cagcaatcgt
    31141 agcagaagct tacaaatata ttggtactcc atatgtttgg ggcggtaaag atccaagtgg
    31201 ctttgactgc tcagggttca cacgctatgt ttacatgcaa gtaactggtc gtgacatcgg
    31261 tggttggaca gttcctcaag aatcagctgg aacaaaaatt tcagtttctc aagcaaaagc
    31321 tggagacttg ttattctggg ggtcacaagg cggaacttac cacgtagcga ttgctttagg
    31381 cggaggacaa tatatccatg ctcctcaacc aggcgaaagc gtaaaagttg gttcagttca
    31441 atggtttgcc cctgactttg ctgtcagcat gtaagaatga ttaatcaaaa agagttagcc
    31501 cattctggac taactctttt ttt
//
LOCUS       DAFEPQ010000025        30586 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.025, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000025
VERSION     DAFEPQ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.025"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..225
                     /locus_tag="LP543_001297"
     CDS             <1..225
                     /locus_tag="LP543_001297"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBL2321472.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
     gene            207..512
                     /gene="kduI"
                     /locus_tag="LP543_001298"
                     /pseudo
     CDS             207..512
                     /gene="kduI"
                     /locus_tag="LP543_001298"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            532..1335
                     /locus_tag="LP543_001299"
     CDS             532..1335
                     /locus_tag="LP543_001299"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBL2321473.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(1584..2648)
                     /locus_tag="LP543_001300"
     CDS             complement(1584..2648)
                     /locus_tag="LP543_001300"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBL2321474.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(2645..3730)
                     /locus_tag="LP543_001301"
     CDS             complement(2645..3730)
                     /locus_tag="LP543_001301"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBL2321475.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(3743..4720)
                     /gene="mvaD"
                     /locus_tag="LP543_001302"
     CDS             complement(3743..4720)
                     /gene="mvaD"
                     /locus_tag="LP543_001302"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBL2321476.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(4713..5657)
                     /gene="mvk"
                     /locus_tag="LP543_001303"
     CDS             complement(4713..5657)
                     /gene="mvk"
                     /locus_tag="LP543_001303"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBL2321477.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5973..6632
                     /locus_tag="LP543_001304"
     CDS             5973..6632
                     /locus_tag="LP543_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL2321478.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            6717..7622
                     /locus_tag="LP543_001305"
     CDS             6717..7622
                     /locus_tag="LP543_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL2321479.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(7624..8256)
                     /locus_tag="LP543_001306"
     CDS             complement(7624..8256)
                     /locus_tag="LP543_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBL2321480.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    8407..8532
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            8576..9100
                     /gene="infC"
                     /locus_tag="LP543_001307"
     CDS             8576..9100
                     /gene="infC"
                     /locus_tag="LP543_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBL2321481.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            9172..9372
                     /gene="rpmI"
                     /locus_tag="LP543_001308"
     CDS             9172..9372
                     /gene="rpmI"
                     /locus_tag="LP543_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBL2321482.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            9425..9784
                     /gene="rplT"
                     /locus_tag="LP543_001309"
     CDS             9425..9784
                     /gene="rplT"
                     /locus_tag="LP543_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBL2321483.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            10136..11374
                     /locus_tag="LP543_001310"
     CDS             10136..11374
                     /locus_tag="LP543_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBL2321484.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAES
                     YTYNDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLL
                     PVLGVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGF
                     LMTLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSA
                     ILAVVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIP
                     TGWIIGAILCLITFLLYGNA"
     gene            11422..13551
                     /locus_tag="LP543_001311"
     CDS             11422..13551
                     /locus_tag="LP543_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBL2321485.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            13526..14215
                     /locus_tag="LP543_001312"
     CDS             13526..14215
                     /locus_tag="LP543_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321486.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEIYEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(14623..15309)
                     /locus_tag="LP543_001313"
     CDS             complement(14623..15309)
                     /locus_tag="LP543_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2321487.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(15445..15639)
                     /locus_tag="LP543_001314"
     CDS             complement(15445..15639)
                     /locus_tag="LP543_001314"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321488.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(15689..16129)
                     /locus_tag="LP543_001315"
     CDS             complement(15689..16129)
                     /locus_tag="LP543_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBL2321489.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(16133..16978)
                     /locus_tag="LP543_001316"
     CDS             complement(16133..16978)
                     /locus_tag="LP543_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBL2321490.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            17127..18545
                     /gene="pepV"
                     /locus_tag="LP543_001317"
     CDS             17127..18545
                     /gene="pepV"
                     /locus_tag="LP543_001317"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBL2321491.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(18621..19823)
                     /locus_tag="LP543_001318"
     CDS             complement(18621..19823)
                     /locus_tag="LP543_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321492.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(19810..21597)
                     /locus_tag="LP543_001319"
     CDS             complement(19810..21597)
                     /locus_tag="LP543_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321493.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(21610..22563)
                     /locus_tag="LP543_001320"
     CDS             complement(21610..22563)
                     /locus_tag="LP543_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL2321494.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(22748..23098)
                     /locus_tag="LP543_001321"
     CDS             complement(22748..23098)
                     /locus_tag="LP543_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBL2321495.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            23298..24377
                     /gene="pepA"
                     /locus_tag="LP543_001322"
     CDS             23298..24377
                     /gene="pepA"
                     /locus_tag="LP543_001322"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBL2321496.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            24520..24840
                     /locus_tag="LP543_001323"
     CDS             24520..24840
                     /locus_tag="LP543_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBL2321497.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            24862..25326
                     /locus_tag="LP543_001324"
     CDS             24862..25326
                     /locus_tag="LP543_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL2321498.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            25522..26127
                     /locus_tag="LP543_001325"
     CDS             25522..26127
                     /locus_tag="LP543_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBL2321499.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(26166..27455)
                     /locus_tag="LP543_001326"
     CDS             complement(26166..27455)
                     /locus_tag="LP543_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBL2321500.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            27883..28362
                     /gene="rlmH"
                     /locus_tag="LP543_001327"
     CDS             27883..28362
                     /gene="rlmH"
                     /locus_tag="LP543_001327"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBL2321501.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            28715..29029
                     /locus_tag="LP543_001328"
     CDS             28715..29029
                     /locus_tag="LP543_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL2321502.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            29045..29431
                     /locus_tag="LP543_001329"
     CDS             29045..29431
                     /locus_tag="LP543_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL2321503.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            29460..>30586
                     /locus_tag="LP543_001330"
     CDS             29460..>30586
                     /locus_tag="LP543_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBL2321504.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRD"
BASE COUNT         9687 a         5721 c         6239 g         8939 t
ORIGIN      
        1 ttgtgggcgc taggaatcat gctagtagca ttcatcgttg ctaaaaagaa taaataccct
       61 gttgtcgaaa aaggacagat ggctcatgct ggaaaaataa taaaagaggc gattcctagc
      121 cttctattga ttgtcatcat tatcggtggt atcttgacgg ggatatttac agcagtagaa
      181 gcttcagcga tagctgtcat ttataaatgg gttatacgat tttagaacca ggaagtgctt
      241 ggaatacgat gccttgtcat acacatgaac gtcgtatgga agcttatgtc tattttgatt
      301 atgccaatga agatacacgt gtgttccata tgatgggcaa accagatgaa acaaaacatt
      361 tagtcgtaga caatgaacaa gccattattt cgccaagctg gtcgatccat tcaggtgtag
      421 gtacaagcaa ttactcattc atctgggcaa tgtgcggaga aaatatcacc tatacagaca
      481 tggatatggt gcaaatggat caattaaaat aagcataagg gagagaaaat gatggaattt
      541 agtatggata tgtttcgttt agatggaaaa gtagcattag tcaccggtgc tgtctatggt
      601 attggatttg agattgcacg ctctttagct acagcaggag caaaaatcgt atttaataac
      661 ttgaatcaag aatcagttga tgaaggtata gcgcattata aagaagcagg catcgatgca
      721 aaaggttatg tctgcgatgt gaccgatgaa gaagcggttc aagcgatggt tcaacagatc
      781 aaagaagatg taggttcagt tgatattcta gtcaacaatg cagggatcat caaacggaca
      841 ccgatgatcg aaatggatgc ggcagacttc cgccaagtaa tcgacgtgga tttaaatgcc
      901 ccattcatcg tttcaaaagc agttattcgg gatatgatcg aaaaaggcgg cggtaaaatc
      961 atcaatatct gctccatgat gagtgaatta ggacgcgaaa cggtcagtgc ttatgctgcg
     1021 gcaaaaggcg gattaaaaat gctgacgaaa aacatcgctt ctgaatacgg tcaatacaat
     1081 atccaatgta atggaattgg accaggttat attgctacac cgcaaactgc tccattacgc
     1141 gaaacacaag aaaacggtga acgtcatcca ttcgatcaat ttatcgttgg acgtacgcca
     1201 gcagcacgct ggggcgagcc aatcgatttg gctggaccat ctgtcttctt ggcttctagc
     1261 gcatctgatt tcgttaacgg ccatgtcttg tatgtggacg gaggcatact tgcctatatc
     1321 ggaaaacaac cttaaaagag ctttagtaca ccgtttagtg gagaggttgt gaaatcagcg
     1381 gcttgaacga ataccgaagg acagctgatt aaacatcatt tattcgaaaa aagaggctgg
     1441 gacagaagcg tttaactcca agaaataaga aggaattcac gaaaattgct tttcaaattt
     1501 ttgtgaattt cagcttattt ccgaaggagt tgcttttgct tccgccgttt atacgtgttt
     1561 agagcgtggg ataaaagtgt tctttacttt tgtcccacgc tcttttctgt gcgtcttcca
     1621 tacttcacta agtcgatccc tcttgcttcg caccaatcct tcactggtcc ggaaaagatc
     1681 agtgattgtt ggtgtagggc cgcggtattc gttgcgccga ccatagtata aagcagacga
     1741 agctcttctt gccattgctt catcagtata atagtttctt ctacgccgtg attcatcaga
     1801 tgcgtcagta cggttcctga agtacccact gcatttgcgc cgagacaaag ggctttaaat
     1861 atgtcatagg cattgcggat acctccggag gctaggattt ccatagaagt gtctgcttca
     1921 tttgcttcaa gcaatgaaga gactgtcgat tgcccccagt cagctaaata actcagttcc
     1981 cgcttggagc gacgggcgtt ttcgatctgg gtgaagcttg ttccgcttcg accgctgata
     2041 tcaactgtat gaactcctaa agcagctaga tcattgagcg tttctcttgt catgccaaaa
     2101 ccgacttctt ttacgatgag ggggacgtca atggctgttt gagtctcttg aataagtact
     2161 ttccaattgg taaaatcacg gtctccctca ggcataacca actcttgagg tgcattcaaa
     2221 tgaatctgta aggcatctgc gtgaaacaat cggatagctt cttgggctct ttcgacagaa
     2281 gtccctgcac cgatgttggc aatgattttc ccgtgaggat attcttgtcg cataatcgta
     2341 taagtatcgg ctaaagaagg gtctttcaag gcagcgctga ctgaaccagt ggcaatcatc
     2401 aaatctgctt ctcttgcgac gattgcaaga tcttggttga ttttttttgt tttttcgctt
     2461 ccgccagtca tcgcattgat ataaaatggg ctgcttagtg taaacccaac tgcctgtgta
     2521 cttagatcga catcagaaac tgctatttgc ggcaaagggt tatggatgat ccgtacgaag
     2581 tcaaattcgt ttttttgttt atcatgaaag gctttagcta gtgaaacatg ttcgtctttt
     2641 cgattcatcg gttttccttt ctttgatcgc tatagacatg taacggcagt ggagtgattt
     2701 ctgctttttc ccatgcactc attaaaggaa ggatgccaga tttctggtca acaattacga
     2761 ttccgcaatc gccaccacct gcaccagaag attttgcggc tccttcatac tgttcagcta
     2821 aattacacaa tttgttcaaa gcaggcgttt cgatgacgac cccagtgatt gcagataaat
     2881 catgcagtaa ttgtcggttt tttcgaatca tctgttggat caacgttaca ttattttctt
     2941 taaacccttt gatcatttcg ttgacacatt ctgtactgtt ttttaagaaa agctgataag
     3001 ccaccatttt gtcttctctc gaacggtgaa cttgatcgac caaatcagaa gtagaggcag
     3061 ggctacccgt ccaaccaatc aataaacgta aatcttcagg agcaatcaat ggctcaatgg
     3121 atagacctgg ccaatccagg gcaagtaact cactgataga atgctgagtt tcttgttctt
     3181 ggagccaagg atggtcgaag gttgagaaag cgatccagcc accataacag ctagcagcga
     3241 tgtcgccgca ggaaccatta tcttgaactg ctaaattggc tagtgctgca atcttgaaaa
     3301 tctccaattg agataaattc aacgcgtaaa aaacattcaa ggctttgacc gttgcaacgg
     3361 tcacggcacc gctagaaccc aatccatatt tccggccatt tgaactgtct aactcacttg
     3421 ttactttcag atcataaaag gataaaagaa tgtttttttc ttgtgcatac ttttcagtca
     3481 aacgaatagc agcaaggata taatgaaaag gattttcccg aatatctaaa actaattctc
     3541 cgttgcgtct tgtccaacgt acaggcatcc cactatattg tgcagattgg atacttccga
     3601 cttttcgtgc ggattctaca gtgactgtta cgaattgatc gactgcagcg ataactgctg
     3661 gatgtcctgt ttcaacaact gcatattctc cggcaatata gagtttgcct ggtgcagata
     3721 cttcaatcat aaaaatctcc ctttattcaa taatcgcaat tcctggacca gcatgggcag
     3781 taattacttg ttctttgcta aataatttgg taaatgtttc ttgtacctca gataaatggc
     3841 tgttttccac taaaactttg acattagggc cagcatccat cgtaaagtag catggaatgc
     3901 ctttttttct caattgccgc accgcatcca tcgcttttaa gctgtcagga gaccagtaag
     3961 tgaatggagg ctgggcagct aaagtcgttc catgcatttt tagtccgttt ctttccatcg
     4021 tttctccgag aagttggaaa tcttttgttt tgattgcttg tttcaattga tatagatccc
     4081 cttcaacgga atctaaccag ccttgataaa aactggatgt ttcgactgtt cgacgcatcc
     4141 catttctgct ggacacttct tttttctggt cgttgatcaa aacgaaaacc attgctaaat
     4201 cgtcttcgaa agaatcggaa gggactggct tagcgtaaga acttaagtcg tcatggcctt
     4261 tttcccattc aacgaaacca ccgaaaatac ttcggcaagc tgaaccagac ccgcgtcgag
     4321 caaatcttga aagagagaga tcgtctaatc caagcttaag agcagtattg caagctcctg
     4381 ctaaagcagc tagcccgctg gcagaggagg ctaatccagc tgcggtagga acgaaattct
     4441 gactgattac tgaagctttt agtgagaggc cggcttcttt acgaaaaagg tcaagaaact
     4501 gactgacttt ttttgttgct ttttcgtctt gtaaggtgcc atctagataa aattcatcta
     4561 ccatatagct atcagaaaag atgacttctg tctctgtata aaatgcatcc aatgttaacg
     4621 aaaggctgtt gttcattgga aggatgagtt cttcattttt cttaccccaa tatttgatta
     4681 gagcaatgtt ggtatatgcg cgtgctttgc ctttaaacat aggtatgtac tcctaatcct
     4741 tgtatccaag tttcagcagc acccgcatct tcaagtgctt gactgatttc ttgggctttt
     4801 gtttttgttt gtgctaatgc aatcatacag ccaccgcgtc caccgccagt taatttggca
     4861 cccaatgccc cggtatcttt tgctgtttgg atcaatagat taagaaaatc attgctgatt
     4921 gtcagctttt ccagaagtga ctgtgattca tccattgtct gtgccaacgt ttctggtgaa
     4981 ttctcaatga ttgcttgttt tgcttgcttc gtcaagtatc ctaatttttg gatcatttgt
     5041 ccagtttcat gaggctgggt ttcaaagagg tgagcgacat ctttgacggc ttctcttgtt
     5101 tgtcctttga ttcctgtatc agcgacaatc aaaaaagcat cgatgttcaa agaaaagtag
     5161 tcaaaaggat gcccttttgt aaaataaatc ggctgcaagc tgctagtagc ggctgcatcg
     5221 attccactag gattaccgtg ggcgattttt tccgaaagct ggacattttc taatagtatt
     5281 tcacgagaca aaggaaatgc taagtaatca taaaaagcac gagtgactgc tgtggcgact
     5341 gcagcgcttg atcccattcc acgttcagca ggaatcgtgc tttcaatggt cagttgcaag
     5401 tttttttgga tgtcatgctg tttttttaag tgaatgaata attttttgat attttttaat
     5461 gcatgaggag cttcggctaa atttcctgaa tagtaggaag aaaccagttg atcgtccatt
     5521 gcatccagct cttcaaggaa tgcggtgact tttgttgcat agaaaggaaa ggcaatcgct
     5581 ggttctccat aaacaaccgc gtgctcgccc atcaatatga tctttccgct tgattctcct
     5641 tggccatagt ttgccataat ctaactcctt ctcacgtaaa agtgattttt aagcaaaaaa
     5701 taaagcgccc aaaacgagtt gcgctgcttc acgaattttt ttgtccacta agggtctcaa
     5761 aaaacattcc tcctttattt tactaaaacc atctcagaag taaaactaag aatacgtttt
     5821 ctaaagaaaa aacacttttt tcttaagaat tttttgactt tttttcttct ttgactagaa
     5881 aaaatacaat tgacactact agatattggg ggtatattta aaaaaggttg gagttaaaca
     5941 caatatttag tgatgaagtg agctggaggt aaatgagcgt gatcgtttta gcaggtacaa
     6001 ttggtgccgg aaaatcaagt ttaacagaaa tgatggctga acactttgat agtcaggcat
     6061 tttatgaatc gattgatgat aatgaagtct tgcccttatt ttatgcaaac cctgaacagt
     6121 atgctttttt gctgcaaatt tattttttga ataaacgttt tgcaagcatc aaacaagcaa
     6181 tgaaagatga caataatgta ttggaccgtt cgatttatga agattcactg cttttccatc
     6241 taaatgcaga tctaggaaga gcaacagaaa cagaagttcg tgtgtatgat gaactgctgg
     6301 aaaacatgat ggaagaactt ccctatgctg cccataaaaa acatccggat ttattagttc
     6361 atattcgtgt atcttttgat acgatgttag agcgtatcga aaaacgtggt cgttcgtatg
     6421 aacaattgtc ctttgaccca agtctatacg attattacaa agaattgaac agacgttatg
     6481 atcaatggta tgaagaatac aaagaaagtc caaaaatcca aatcgatgga gaccgctata
     6541 attttgtgga agatcctcaa gcaaaagaag aagtcttgaa aatgatcgaa gaaaaattag
     6601 cagagattcg ccaaaccaaa gtatccattt gatttacagt tgctcaaata aagggtaaac
     6661 ctttatttga gcgacttttt tctttatact ggaaacaaat gtcaggagga tagagtatgc
     6721 gggaaaaaca ctattttaat caagaccgta atttttggat caatgtacag caaaacgatg
     6781 tgctagctgt taaaaactta aaagaagaat atggcattag tgacgagatg ctgacttatt
     6841 ctctagataa aaatgaacgg gcgcgcgtag agtacgattc ttttgatgaa gcgttactgc
     6901 ttgtatcaaa tgttccgcac cagcaaaaag tcgataatca ttatgaaact agtcctatcg
     6961 cttttatttt aaaagaagac ggtttgttta catttaccac gccgaatacc gagtacgtga
     7021 ttcgactaat tcgatcgctt ttggaacgga tgccagacat gagtgtatac agtttattgt
     7081 ttcgtacatt gtttttgatt tctgattcct tttttccttt aatcgaggaa gtgaatagtg
     7141 aacgtcagcg gcttaatctg aaattaagag aaaaaacaac gaacaagaat ctgctgcaac
     7201 tttcagattt agagatcggt ttggtttact tagttacagg aacaaaacaa aatgtagtac
     7261 ttttagaaca aatcaaggca ctggctattt atcgcaagtt gtcagaaaag gaaaaagaag
     7321 agttggacga tgcactgatc gaagcacgac aagcagttga aatgacgaac ttagcttcgc
     7381 agattttaga ccagctctct ggcacttata ataatttgct gaataacaac ttgaatgaca
     7441 cgatgaaatt tttgacggtc tggtcgctga tcttgaccgt tccaactatt gtgacaggat
     7501 tcttcggaat gaatctccag ttacccttta cccattccgt tttcggatgg gaaatcgcct
     7561 tgatcatcag tttggtctta tctatttgga tgcttattgc actgtggcgg cggattcgtt
     7621 gatctagtct ttgatctgca aaccaagggc ttgggcgatc acaagtgctt gctcttgatt
     7681 gacgattagt cccgccaaag aatcttgaga taaagcgatt gttgtgaaga aattagtcgt
     7741 aaagtctaat cccttcagag aagttcgaaa ccagttcgaa ccggttaact gattgtcaga
     7801 caaaaaaagg tttttccaag tgacctcata aaattcgctg tcttctaatt gacaatgatc
     7861 gaaggagaca gctttcaagt ttgtactact aaaggaagcc atcgaagcaa tacaatcagt
     7921 gaaagtacag tctcgaagat aactttcggc aaaattcgta cctgttaatt tacattgatg
     7981 aaacacgact tggtggaaac tgccaccaat ccactccaga ttggaaaaat cacatttttc
     8041 aaaaatgaca ttactgcatt caaaatgacg taaacctgtt ttttgcatcg taagtttttg
     8101 gatatgactc tctcttaata ccatattttt ggcatcgaga tagctttggt tacctccgtt
     8161 gatcagaaga ccagagaaga tctcctcatc ttctaaataa aactctccct ttgtaagaag
     8221 aggaagagaa ggtggaatag gttcgatttt tttcatgtgg gttcacctcg tttttcttta
     8281 gtatagcaaa gaagcgggaa tcactgcaag aaagcctgct aatctaatca ttttcagaaa
     8341 aggaagacaa actgtatgaa aaacattgac aaaaagatta gtttttgcta tactataatg
     8401 gttgagaaat aaaagcagaa gcacccgctt ctcgccttag ttgataatgt cactgggcta
     8461 gatagttttc actggttcta ttaggtgaag tacgcgcggg ttctgtaaga gaactcgttt
     8521 ttttattgcc gtttattcgg taagcttttc tttctctcat aatatttgga ggtgaatgac
     8581 catagcaaag gatatgatgg ttaacgacgg cattcgtgca cgagagttac gattgatcgg
     8641 ttcagatggt gagcaattag gagttaagac aaaagcagaa gcattgaata ttgcagaaca
     8701 agcaaacttg gatcttgtgt tagttgcccc tggtgcgaaa ccaccagttg cgcgaatcat
     8761 ggactacggt aaataccgtt tcgaacaaca aaagaaagac cgcgaagcgc gtaaaaaaca
     8821 aaaagtgatc aatgtaaaag aagttcgctt aagtcctacg atcgacttga atgacttcaa
     8881 tacaaaactt cgtaatgcac gtaagttctt ggaaaagggc gataaagtga aagcttctat
     8941 ccgtttcaaa ggccgtgcca ttacccataa agaaattggt caaaaagtct tgaatcgctt
     9001 agctgaagaa actgcggata ttgcaacagt ggaacaaaaa gcgaaaatgg acggacgcag
     9061 catgttcttg acgctggcac cgaaaaacga cagtaagtaa tttgaatgac taactggctg
     9121 ttggacagcc gatagtcgga taacgaaaga ttttgaggag gaaatatagt catgccaaaa
     9181 caaaaaacac accgcggatc agcaaaacgt ttcaaacgta ctggtaaagg cggattgaaa
     9241 cgcttccgcg cgtttacaag tcaccgtttc cacggcaaaa caaaaaaaca acgccgtcaa
     9301 ttgcgtaaag caagcatggt ttctagtggc gacttcaaac gtattcgcca acaactagca
     9361 aaaatgaaat aagaagtaca aataacagaa tctgaagatt tatttttatt aggaggaatt
     9421 atacatggca cgtgttaaag gtggaacagt aactcgtaaa cgtcgtaaaa gagtgctaaa
     9481 attagcaaaa ggttactacg gttcaaaaca cactttattt aaaacagcaa aagaacaagt
     9541 aatgaattca tacaactacg catatcgcga tcgtcgtcaa aagaaacgcg acttccgtaa
     9601 attgtggatt gctcgtatca acgcagcagc tcgtatgaat ggcttgagct actcaaaatt
     9661 aatgcacggt ttgaaagttg ctgaaatcga catcaaccgt aaaatgttag ctgacttggc
     9721 tgttaacgat gcagcagcat ttactgcatt ggctgaccaa gcaaaagaag ctttggctaa
     9781 ataagctaaa caattataaa gaggaaagaa gataacgaaa agttatgttc tttcctcttt
     9841 ttttatttac aatcctccat caaatgaaac aggacaataa cgtataagtc taaaataaca
     9901 taattaaggt tttttaattg aaaactgaac atttatggtc atcctttaat gaaagcgcta
     9961 actacataag ttagaatgag tttgactgat atattagttt gcttatcaga aaaacaaaat
    10021 ggacggagga ataacatgag tgatattatt attggtattt tgttagttat ctcattcttt
    10081 ttcatggtct ggtactgtgt aaaaggctac aacttgatgg tcggcttttg tcatcatggc
    10141 tactgtatgg atgggattag cgctagtcgg aaacacgttt tcaccaaact cggcgatgga
    10201 agggcaagga gtaattgatg tgttgacgca tatctatacg acggggccag ctgagtacgc
    10261 caaatcgatc ttggtaaatg tcttctttgg tgcctttttc gggcgtgttc tagtagatag
    10321 cggaatcgca gctaccctga ttcgaaaggt agttgaactt ggaggggaca agccaagaat
    10381 cacgatgagc ttactatgta ttgtaaccgc tgttatattc atgtcgatga caggaatcgg
    10441 accagtgatt tctattgcag ttattgtgtt gccgatcttg atgtcattag gtatttcagt
    10501 accggttgca ctgttctcat ttatgggctc gattatggct ggaatctttg ctaatatcgt
    10561 caatttcaaa caatatcaaa cgatttatgc tggtttcaat ccagcggctg aaagctatac
    10621 ttataatgac tatttccaaa ttggtatgat tgggatgatt gtaagcttag ttgtggtact
    10681 aacggtcgct aatatatcaa tgaacaaaaa gaaacgctat gcaatggcag caaacgtacc
    10741 agctgaaggt ggcgacgcac caatgatctc atggttagcc gtattattac cagttttagg
    10801 ggtcgttctt ttagatcttc aaatcatctt aggttttatt ctagcaggta tctgggcatt
    10861 gctgtttact ggaaaattgc gcggaggtta taaagaaatc tgtcgtcagt ttgcgaaatt
    10921 atttacagat ggcgcagtcg atgttgcacc aatggttggt ttcttgatga ccttagcgat
    10981 gttcaataat tcagctgcgt atgcttctcc ttatttttca gctatttttg gagattggat
    11041 accgcaatca ccattagtct tggcaatcgt atttgctata ttgacaccac taggattttt
    11101 ccgcggtcca atgaatctag tcggttcagg ctcagctatt ttagctgtcg tgttagctgt
    11161 taatccaacg atgtcgccag cctttttgtt cccgttattt gcaattacaa cgattgctcc
    11221 tcagcattta gatattacac aatcttgggt tgcttgggga ttaggttata caaaagtaac
    11281 ttctcgagaa tatatgaaga aatccatccc aactggttgg atcatcggtg cgatcttatg
    11341 cctgatcacg ttcttattgt acgggaacgc ctagtaaaca aaacgtcgat aaaaacgatt
    11401 agataaatgg aggaaaagaa aatgaaacgt tttgtgcgaa tgggaattga tgttggcggt
    11461 acacatacga aagcagtcgc aatcgataat gcgacgcacg aaatcatcgg aaaatcttca
    11521 gtgaaaacaa cccacgatga tgtccgtgga gtagccgcag gtgtcgttca gtcttttcaa
    11581 aattgtttgc gggaaaataa tatcagtcca gaagatgttg tgtttgttgc acattctacc
    11641 acacaagcga ctaatgcact gattgaagga gatgtagcaa aagtcggcgt aatcgggatg
    11701 gcaaaaggtg gattagaagg ttttttagca aaacgacaaa ctagattgaa cgatatcgat
    11761 ttagggaata agaaaaaaat agagattgtc aatgcattct tacctgtcaa aaacttaaat
    11821 gtagaccgtg tttcagaaac gatttcttct ttagaaagag aaagagcgga ggtcttggtt
    11881 tcttctatgg catttggagt agataatggg gaaccagaac gagttgttta tgaagcagca
    11941 tctgtgaaag cgatccctac aacgatggct tctgacatca caaaattata tggattgaca
    12001 agaagaactc ggacagcagc aatcaacgca tctattttac caaaaatgct agatacggca
    12061 acctcgacag aagactccgt acgagaagca ggggtcaatg tttctttgat gattatgcgc
    12121 ggagacggcg gagtaatgga aatcaatgaa atgaaaaaac gacctgtttt aaccatgctt
    12181 tccggtcctg ctgcatcagt catgggctct ttgatgtact tgagagcgtc taatggtgtt
    12241 tattttgaag taggcggaac aacaaccaat atcggggtca ttaaaaatgg acgtccagca
    12301 atcgattatt caattgtagg tggccaccca acatatatct cttcattaga tgttcgggtt
    12361 ctaggtgtgg caggcggttc gatggtacgg gctaatcagt caggaatcat cgatgtcggt
    12421 ccacgttctg cacatattgc cggtctagat tatgcagtat ttacggagac agaaaaaatc
    12481 aaaggtccta aggttgaatt tttctctcct aaagaaggag accctgctga ttacgtcaag
    12541 gtagtgatgg aagacggaga agaagtgact attacaaata catgtgcagc caatgttctt
    12601 ggattagtgc aagaagaaca tttttcttat ggaaatgtcc catctgcacg aaaagccata
    12661 caggcattag cagactattg tcatacgacg gttgaagaca tcgcagagca gatcatggaa
    12721 aaatcctatg ctaagattga accggtgatt ttagaattgg cagacaagta tcacttggag
    12781 aaagatcaga tttctcttgt aggtgtaggg ggcggcgctg cctcattgat tacttacttt
    12841 agtaataaaa tgggtgtgaa atattcgatt cctgaaaacg ccgaagtgat ttcttctatt
    12901 ggtgtagcac ttgcaatggt ccgagatgtt gtagaacgta tcatcccttc gccaagtaaa
    12961 gaagatatac gatcgttgaa aaatgaagcg atgaataaag cgattgaatc aggcgctaca
    13021 ccagaatcga tcgaagtcca cgtagaaatc gatccacata cttcaaaagt aacggctatc
    13081 gctactggtt ctacggaagt gaaagcaacc gatctaacca aggagatcac aacagaagaa
    13141 gcactagagc ttgctgcaga ggatatgcga ttaaataaaa atgaagtttg cttattggaa
    13201 aatacacctt tcttttatgt atgcggcgaa cagaatcgtt caaaaaatgc tggaagtctt
    13261 cggatcatcg atcaaaaagg atttatcaag gtccaacgtg gacatgcttc atgtatgaaa
    13321 acaactgcag ccaattatat gacagctgtc gaacaacttt gggaagatat ggcagtttat
    13381 caaactgaat tgattgcccg accagaattt tatctctgct taggagcaag agtctctgat
    13441 tttacagcga cggatctgga acaactacag cttttgatgg atctggaagt atctactatg
    13501 gaaccggagg aagaagtgat cgtcgttgcc ggaaatatca aacaaacata agttagaaga
    13561 gttactaaac gaattacaac agatccccga agaaatttgg ggattctacc agtttcggcg
    13621 agatttattt tggaaaaaga ttcctttgtc caagcagaaa attcttatac aacaatcaat
    13681 cgattgtggt atcgaaacgg catgtagcat caaaaaaaag tacccttttg ccgatgtagg
    13741 agaaatctat gaacaaatgg ctgttccaat cgtttcctgt gaatcagagc aaataaacga
    13801 acgaatcact tttgcaactt atgcagaaga tgaagggatc cgattgatga cggagccgtt
    13861 agaaaaatta aaatgttcag gactagcttc gatctctaaa gaaacagcgc aagctttgat
    13921 tattggacat gaactatttc accacatcga agcttctgtt aagggaattt atacgcaaag
    13981 cgaaaaaatt gtgctgtggc ggttgccttt ttatacacat caatcaaaca ttcgtgcatt
    14041 gagcgagata gcagcgatga gtttttcaaa agagatgaat caaagcgatt tttcgcctta
    14101 tgtattagag gcagtcttac tttggccata taatgaaatt cgtagccaag ggatcttaga
    14161 agaaataaaa gaaatagaaa aaagatgtac ggagtatgat ttagattaca aataaagaga
    14221 ctgtgacaaa agtcgtgtca cagtcctttt tttccgaata aacggtgttc tcctgtcaac
    14281 tcctcggtca caagtcaaaa tattcaacaa acatgagaag ctgttttttg aaattgttcc
    14341 ttgtgactcg gagatgaacg gcaggctcac gacctcttgg aaaaaggtgt tcaaaagctt
    14401 gcatctgcgg taataagaac agccaagtta aaaacataag aagctatttt tacttggctg
    14461 ttcttattac ttgatgcagg gcagcttttt cacaacctct caataaacgg tgtgcaatca
    14521 gctgaccctc ggtattagct caaaaaaaac ggcaaaatat gaggaactat tttgccgttt
    14581 atttctctaa tactcaggtc aaaacgctga ttccaaaacc tcttatttga tgggtctgtc
    14641 ctgaatatct tcaaaataat cggggtaatt atgaatcacg tattcttgct cctcgataat
    14701 catatgtaaa tgcatcgtgc aaagaaatcc gacttcatca aagttatgat caatcattga
    14761 ttgaagcatt gtctgatgtt catctaatac tcgaccccag tctaattctg aaatcgtcaa
    14821 acgtaaccat ctgaaacggt tcaagtgcgt actataactt tctagccaat cccaaatttc
    14881 tcgttttcca gcaatttcat aacaagttcg gtgaaaatca gtatccaaat caaaaaaagc
    14941 ttttttattt tgagcgatca ctgcctgatc ggtttgttct agtatttttt ctagtttagc
    15001 ttgatcttgc ttggtcattt tcgcactgca ttcttgcagg atcggttttt ccaacttttc
    15061 ccgcgtataa cgggctaatg aagcagatcg gatatcgatt tttgaaacat aagtgccgga
    15121 ctgtggaatg gtttttacaa gctcttgatt gcgcagttgg atcagtgctt ctcgaatcgg
    15181 tgtacgcccg atagctagtt gttcttctaa cactttatcg gagattttac ttccaggtgc
    15241 taactcggaa taaatgattt gtttacgtat agattcataa gcttgctgtt gcaaattatt
    15301 gataaccatt taaccttcct ttctatgcag atataatcta ttataacata aatcgcttac
    15361 aaatcgtgtg ggcataaaaa cacagtctgc aaaaaaagcc ctgataggca tgcctattag
    15421 agcctcgaaa agaaaaatat tgaatcaatc tggtcgtacc tcttggatcg gtttgacacg
    15481 accgccgatt cttttagcaa aagcttcgga ttcttttttg tctttgaaaa tgagcagatt
    15541 gtctaagttt ttttcccgag tgcgacagcc agttttatcg atgtaaccgt gcattacttt
    15601 aacgacatac atggggatca cctgcttttt tcttttcatt atacgaacta ctcgacacaa
    15661 tacaagaaaa atgcttagac ggtatttatt aagaatttac atcatccaaa gaagcgtacg
    15721 cttggaataa ctgttctaac tccggattga ctttgctcag cttcactaat cgttgcgaat
    15781 cagaaagtaa aaaacagtgt ttactactgc cagtagtttt taattttttg tcttcagttc
    15841 ccacaatttc gtagctaaaa tccaaccgtg ttcccgtata cttttggata gttgggtgga
    15901 tgctcacttt ttctccgtaa tggatcattt ctttatagct gcaatttact tccagaacgg
    15961 ggatgatgat cccagattct tcgatttttt tatagggaaa ccctagatgt accaataagg
    16021 ctacccgagc ctcttcaaac cagcggatat aattgctgtg atgaataatc cccatttgat
    16081 ctgtttcata ataaaaaggt tctcgcacat aacctgtaaa tttctccatg actcatgcct
    16141 cgttttccat tgatttttta caaaaataat tcatccactt cggaagtgct tcgctgattt
    16201 gtgtcggcaa aacaatatat tgttcttttg ccagctggtc tccaatgaaa ctatgaagat
    16261 aaacagctgc tgcaatcgtt tggtcatttt gaggaaactg ggctaaaaat cctgtgatca
    16321 tcccagctaa agtgtcaccc atgccgcctg ttgccatagc agcagaacct aaaggattgg
    16381 tgtagaagtg ctggtgacca taaatttttg tctcatgact tttcaaaacg agcacagcac
    16441 cgatttcctg ttgcttcttt ttgttttctt tatcccgttg ttcttgtatc ggtagagaac
    16501 tcaaccgttg ccactccatt tgatgaggtg taagtacagt ttgttcggga tagggtagca
    16561 aaagatgatt agcaacaagt aaacttaaag cagagccatc aatcaccagc cattgttctt
    16621 ttgattgttt ttctagtact tgtttgaaac gttccaaact cttagaagaa gtacccaatc
    16681 cgggaccaat caagagtaca tctgcttgct ttattacgga aagaaactgg tcagtatccg
    16741 tccaatctac aatcattgct tctggcagat gactgtgaag agcactccga tttttttcat
    16801 cagttactac agtcgtgaga cccgctccac tgtgtacggc agctaaagca ctcataatga
    16861 ttgctccgcc atattgttca ttgccgccaa tcaaaacgat acgtccaaat gttcctttat
    16921 gtgaaatctt tggacgaggt gtgatgactc ttttcactag ttgttcgttt atcagcatcg
    16981 aaaaacctcc ttttactcat tatagcgtat atttttttta gagaaaaaca gataagaaaa
    17041 ggaattgtca gattttttga actatttttt tctgtaattt gtggtatcat gaacacgaag
    17101 acttaaataa gtggaggatt aactttatga caattgattg gcaaaaagaa gtagaagcac
    17161 gtaaagaaga cttgcttgaa gacttgaaaa atcttttacg tgtcaacagc gagcgcgatg
    17221 attcaaaagt aacaccagat gcaccatttg gacctggacc tcgcgacgca ttgaaacata
    17281 tgttagctta tggcgaacgt gatggattta aagtaaaaaa tgtcgataac tatgcagggc
    17341 atattgatct tggcgaagga gacgaaacgc tagggatatt tggacacatg gatgtcgttc
    17401 ctgcaggaga tggctgggac acagatcctt atgaaccagt aatcaaggat ggaaagatct
    17461 ttgcacgcgg atcaagcgat gacaaaggtc caagtatggc agcttactat gcgatgaaga
    17521 tcattaaaga acttgacttg aaactatcaa aaaaagttcg cttcgtagtc ggcagcgatg
    17581 aagaaagcgg ctggggcgat atggcttatt atttcgaaca tgaagaagaa ccagattttg
    17641 gtttctcacc agatgctgaa ttcccaatca tcaatggtga aaaaggaaat gtttctttag
    17701 ctttacgctt caaaggcgac aatgcaggag attatgtctt gaaatctttt gtttccggac
    17761 ttcgcgaaaa tatggtgcca ggaacagcaa ctgcagcact tcaagtacca agtgcagatg
    17821 cagctatcgc gatggaagaa gcattttacc aattcatcga agcaaatcca gtaagcggaa
    17881 cgattgaagc ggacaatacg tatgtaaaaa tcgaattagt tggtaaaggc gcacatggtg
    17941 caagcccaca atctggtatc aatgcaggtt ctttcttggc attgttctta gataactatg
    18001 aattcctagg atctgctaaa caatttatcc atgtagcagc tgcttatgtc catgaagatt
    18061 tttatggaga aaaactagga gtcgcttatg aagatgaaaa aatgggcaaa ttgacaatga
    18121 atgctggact atttgctttt gaagaaaatg gtacagagga agcgaacttc atcaatatga
    18181 atttccgttt tccaaaaggt gtaacagtag acggattgca aagtgacatc gaacaaacag
    18241 tggggcaaga aggcgcaaca gttactcgag gtgctcgcgt aatggaacca cattacgtac
    18301 caatggatga tccattggtg gctacattat tgcaagtcta tgaagaccac actggagaaa
    18361 aaggctacga acaaatcatc ggtggcggaa cttacggacg tttgttgaaa cgtggcgttg
    18421 cttatggagc gatgttccca ggatatactg atacaatgca tcaagcaaac gaatttatga
    18481 gtcttgatga cttgttcaga gcaacagcaa tctatgcaga tgccatctat cgtttggcga
    18541 aataaaaata ctaaaaaact ccggctatct ctgacagctg gagtttttat gtttaaaaat
    18601 gaattagtac atcggtattc ttaaaagtaa tttagttcgt cttttgattt ctcaggaggg
    18661 atcaaaaagg cgaaagcact aagtatagca aaacttgtga tgatttgcgt acgatacgta
    18721 aaccaataat gaatctgtga gtgattagct aaaatattgt accaaatata cggcgttaca
    18781 gcagctaaaa caaacaaaac tagaagttgg aacggacgcc agctatttcg tttacgataa
    18841 gcaaaataga gaaaaacaag acaagtaatc aatagcaata atattgctac ttttggatac
    18901 atcaatctaa tattgctttt cagcatctct aatcgatgca acgggtattt gtcgtttcct
    18961 tccgttcgaa agaggatttg gttgattgca tcttttacaa tgtttttgtt caatatgatc
    19021 gatgatagga tccatttagc cacccatgtc aaaccatagc ctaatcccca aaagataccg
    19081 gtaaaaacaa aactgcctac ttgtttgctc aatgaactgc gtttatccat taaatactta
    19141 tttgttacat aataaataat gatcacaggt atcccccaag agatgaccgg tgttgtcaac
    19201 aagtcaaaaa agttagctaa agaaccaatg acaaagaaat acaagaataa ttgttggttg
    19261 gtgttggaga gttgtggttt tgttaaaaat agtaatacag acaataaagt caaaatccaa
    19321 atatttgaaa attgcatact gaagaaaaag gttgcagcat ttcctatagc aagcgaaatc
    19381 aacaaggctg tcgttaaata gatgtcgcta cacttggtta aaaagtagct cgtcaatcca
    19441 atcagcagaa aaagaatggc tgcgtaaatc attcgaatac tccccaaact catccctagc
    19501 agtaaaggtc gtaaaaaaag ctgataacca tgccaataac ggggataatc cgccatagac
    19561 atcgcgttct ccaaggagtt caattcagga tctgcttttg tttttcgtat catcaaatga
    19621 tcagtaaaat tatctaaccg agtgcctagc atatttcctt gattgataga aggatacatt
    19681 ccttcttttt caaccatctg tagagatttt tctgcattcg attcaataga gtgctgcgga
    19741 atgaaaaaag taattgtcag tagtacgcaa tagctagcga tcaagagaac aaaagcaaaa
    19801 aatatctttt taaatagttt catgagtatc tcctatatct gttttcattt tttctctttt
    19861 atctagtaaa aaaggagaga ggatataaac aggtagacct aatgctaaaa caaacacata
    19921 ccgtaaacta aaagccacag gagtagcaat cataatagta gcaaaaacaa gcataccggg
    19981 aagaaaaagg agtaaatacc gatatttttt cttaaagatt gctaaaagtg cactgccgat
    20041 agctaaccac agtaatgtgc cggaaccaaa gaaatcacga gcatcgatga tttttttcat
    20101 tcctgacaat ccagtaagat gttcaatcaa atctacttgc ttcataccat aattattcgt
    20161 gtttacacga gtatctaaaa agccgtaact attttttgta ccaggtaccc agaacccgaa
    20221 cgtttctaaa atataagcat ctgtatagat ttttatatta cttggtaaat ttcttgccca
    20281 tagtgataga aattcttttt tatgtgtatt caaaaactca ttgttaaaat ttttatgcca
    20341 cttgattgag tctgcaagac taggagaata cgcttcataa tcttttatcg ggagtagctg
    20401 attcatgaat gctttatctt tcttagacaa gtcaccatcc ataacaatag ttctagaaat
    20461 ttgctgcaaa ggaacaccta attgttcagc agtttctgta ggaatagcat aacgagccat
    20521 caacgggtgg atgagtagtc cagcaatcac tgtataaccc agcaccactt gaaagaatgc
    20581 ttgatacctt ttttgtttca aaataatcaa gccgcaaatc aaacctgtgc cggttactac
    20641 ataaatccca ttatttctta gataggtggt cagtaaagca aggatgatta cgtaaatctg
    20701 attgagaatc gttggctttt ctcgttgaaa aataaagtca aaaaccgcta aggaaaataa
    20761 aaagcaagcg atagagaaca aagtatcttt ttgcatgttc aaggcgtaga ttggaaaaac
    20821 cggtgcaaat gcaaaatagc ataaactaat gagtaccgga aaaaaatgga tgtttctttt
    20881 taacagccag caaagagaat aacttatcgc tgaagctgtt atcaatgttt gaaaaatcga
    20941 aaataaaaat acaccgtgat tgatcgtatc agttggtaaa tcatgaacaa atagcttgac
    21001 cagtaaggtg aataagactg gatgatgatt gcttatagga acatcaccca tagattgggc
    21061 gaccgaactt aacgaatcag gcaaaaccac tccaggatac aatgccaaca gataaggaag
    21121 ccaacacaga actaaaaaga cagtaaagaa taaaaaatat tttcttgaag agaaagcctg
    21181 tgtgttttga gcagtaaaaa atgtttccag ttttggcatt gataaataaa tcgattgtaa
    21241 aaataaaaaa gaatacacaa aaatggagaa ataaacaaga aagtaaaaaa tacttccatc
    21301 agcaatcgtt gtctttgtat agtttgctga gacatactga aattgtatct gtctgccaat
    21361 agaaagtgta agagcaaaag ctgcagaaaa aaacaatcgg gcaatagctg caactttgtt
    21421 ttttattgaa aacaactcta ttccatattg cttaaaagca agtagcaaaa ccatggctaa
    21481 accaatgatt actgttggat tcaaagttcc tagaccataa gaagtaaaaa aagcaaaacc
    21541 tgacgcaaat aaagttgcaa aaaaagtagg aaatagattc tcctttttct tatgcataaa
    21601 aaaggcctcc tacttcattt ctttattttg atattcgtgg tagacatggt ataaattcaa
    21661 ttcatattct ttcttggcat ttgtgacgac cgtatccaaa atcaaccctg ttactaatga
    21721 aagaagggct aaagtcatca atccagtact taaaatcgca gaagggatgc gtgtaataaa
    21781 gcttgtcagc atatattcac gaatcacagg aatccccatg accaatccaa ataaaaagaa
    21841 gaagatggtc cagatcccaa agaaaagcaa aggtttataa tctttgcaca ttttgatgat
    21901 catcatgatc actttgaatc cgtcagagaa tgtattcaat tttgattcgc taccttcagg
    21961 acgatcgcga taatcgattg gaatctcaac gaacttgaat tttttatcta aagcatgaat
    22021 cgtcaattct gtttcgattt gaaaaccaga actcataata gggaagcttt tgacaaaaat
    22081 tcgattgaat cctcgatagc cggtcatgac atcgcgaatt tttgttttat ataagtaagt
    22141 aattgtattt ttaaccagat tattcccaaa atcatggaaa ggacgcttat tttcatcaaa
    22201 ataggttcca tttgatagac gatcgccaat gaccatatct gcttcgccag aacgtaattt
    22261 ctcaagcaaa ccatgtgcag cttctgcagg ataagtatca tcaccatcta ccatcaaata
    22321 ataatcggca tcaatatcaa aaaacatttg acggattaca ttcccttttc cttgacgtgg
    22381 ttctttcttt acgattgctc cgccttgaac ggccaattcg tatgttcgat cggtcgaatt
    22441 attatcatag acataaatat ctgcttctgg taattccctt ttaaaatctt caataacagt
    22501 tgaaattgtc gcctcttcgt tataacaagg aatgagcacg gcaatttttt cacgtgattc
    22561 catttgtcac tctccttata taataaaact aatcttattt caataatacc gtataataac
    22621 tacaaatact agctattcca agaggaaata gccctatttt tataaaaatc acccactata
    22681 aaagaaggat aggatacctc tcttttagat agtgggtgcg tattcgttat ttcatttttt
    22741 tgacgtatta atctattgcg cccttttttc ttgaaatgtc taatacggtt cctgtatgag
    22801 catctgctaa aaattcataa taaacagttt cgccatcttc tatccgggaa agcccacccc
    22861 gataagcact cgaccgaacg gcgaatttcc gaataggttc tttttcaaaa gagatccaag
    22921 caccttcgat tgcgccttct tttaaaaaag atgttttgat attttcaagc acttggtctg
    22981 ctgataacac ttttttgcga tgataccaaa tcgttgaagc taccccactc aataatccta
    23041 gaccgatccc aacggctaac ccgcctttaa aatagcttaa ttcgctttct tcatacatgt
    23101 ggatatacct ccttcttctc acttgctact ctttgtgttt attttaccac actctcatat
    23161 tttctttgaa ataaataacc gctaccagaa aaaataagct ataataaata cgatcggtct
    23221 gcagaaaaaa tcatatttta tccgcagatc aatataagtc tgagaggagt ttagtctgaa
    23281 atttagacga atagcatatg gaagaaaaaa catttcaacg aatcaaagag ttaacagaat
    23341 tacaaggaac aagcggtttt gaacaagacg ttcgcgccta tatggaaagt catatggaac
    23401 cgctggtaga tgagttacag ttagatggtt tgggcggcat ttttgggctg cgccaccatg
    23461 aagaagcgga tgcacctcgt gtaatggtcg cagcacatat ggatgaagtc ggattcatgc
    23521 tgacacaaat ccaagaaaac gggcttttta aagtcgtacc tctaggcgga tggaaccctt
    23581 acgttgtttc tgctcaacgt tttacactta aaacctctac tggtaagaat tatccatgta
    23641 tttcttcttc cgttccccca catttattac gaggaacaag cggacaaaaa tcagtagaag
    23701 taacagatat tttgttcgat gctgggtttg aatcaagaga agaggcaatg agttacggtg
    23761 ttcttccagg agacacaatc gttccttacg ctgaaacgat caaaacagcc aacgggaaga
    23821 atatcatcag caaatcgtgg gacaaccgct atggctgtac aatggtatta gaagctttag
    23881 aagcgttaca aaatgaaaca ttaggtcata cattgattgc aggcgcgaat gtccaagaag
    23941 aagtaggctt gcgtggttct aaagcttctg tcaataaatt caagccagat ctgttctttg
    24001 cagtagactg ctctgcagca gatgacacag tgacgaaaaa tggcacattc ggacatttag
    24061 gagaaggtac attgatgcgt atccaagatc caggtttgat catgcttcct cgtttgcgtg
    24121 agtacttgct agatatcgct gaaacaaaca acatccctta tcaatacttt gtttcaaaag
    24181 gcggtacaga tgcaggtgct gctcatacac aaaatgaagg aatcccaagt acagtgattg
    24241 gcgtagtggg acgttatatc catacgcatc aaacgatgtt cagtattcgc gattttgaag
    24301 ctgcacgaga aatgttgatt caaacattga aaggtttaga taaatcaaca gtgaacacga
    24361 tcgtatacgg gaaataacgc gatagtgaaa ctataaaatc tggcaaaagc aaccttttgt
    24421 cagatttttt ttataaaaag aggatcaatg tttgttagtt cgaaagaaag gtgtataatt
    24481 taattgaaaa atacataaac agaaagtaga ggaaaaagaa tgattatccc aaatagttta
    24541 gaagaattag caggctacgt agaaactgga aaaagtgtat ttttctttac tgccggctgg
    24601 tgcggagact gccgttttat caaaccgcaa atgcctgaaa tcgaaaatga cttttctgat
    24661 tggcaattta ttgaagtaga ccgtgacaag tacatagatg tagccgctga atggaacatt
    24721 tttggtatcc caagttttgt cgtgatccaa gacggaaaag aattaggtcg tctagtgaat
    24781 aaagatcgta aaacaaaaca ggaaatcgaa tcgttcctag aaagtgtaat aaaaggctga
    24841 catctgttag gaggggtcaa gatgaatcaa gaatataaga acattttagt aggtattgac
    24901 ggtagtgaac aagcacttgc tgctttcaaa aaagcgttag aagtggctcg ccgtaataat
    24961 gggaaagtat atgtagcaaa cgtaatcgat cagcaatttt ataattttat gggctatgct
    25021 cctattgatc aaaatctagt cgatcagcaa actgaaacag ccaagcaaat gatcgaagaa
    25081 tgtaaagaat atggaaaatc tgttgattac acaaatatag aaggaattat tgcatacggc
    25141 tctgttcgtg aatccatggc agtcaaattg ccaaaaaaat atgcaatcga tctaatcatg
    25201 gttgggcagt ctggactaaa tgcagtcgaa cgctttatga caggaagtgt agccagctac
    25261 gtcatacgtc attcaccttg tgatgtcttg atcgtttctg acgaagaaga acagccaagt
    25321 aattaagcaa gtggaacttg tataaccttt ttaatcaaac agtaaaggga ctgtgacaaa
    25381 agtcttgtca cagtcccttg ttacgaataa atggtgttcc agaagaagct tcttcggaaa
    25441 taaagcatga aaaaaatctc atgcagtgaa tgcaattttt tgttaagata aatctcagca
    25501 aaggataatg gaggaaagaa catgattttt gcttataaca aagcacacgt cggagacact
    25561 ttgatggtta tcatagccga cgataaagga acagaaaaca ctgtcgaacg taaatggaat
    25621 gttgctcgga tcaaagatga acaaggtcaa gttgttgctt ggaacttttt ccatatctcc
    25681 gatcatctaa caattgaagg gaacggccaa gtaagcttaa cagatgaaga attagcgaaa
    25741 ttgaatcgat tgattaaaga agcaggattc gaagaacagc ttgaagctga tcatgaaccg
    25801 aagatcgttg tcgggtatgt aaaaacatgc gtcccgcatc ctgactctga ccatttatcg
    25861 attactgaga cagaagtgga caatgggaca gtattgcaaa ttgtttgcgg tgcaccaaat
    25921 atcgaagctg gacaaaaagt tgtcgttgca aaaccaggag ccatgatgcc agatggaatg
    25981 atgatctggc caggaacttt gcgaggggta gccagctatg gaatgatttg ttctgctcgt
    26041 gaattgcatt tgccaaatgc tccagaaaaa aaaggaatct tagtattgcc tgaggatgcc
    26101 gtagtcggag aagtatttcc aaaataaaga ggttgtgaca agtctttgtc acaacctctt
    26161 tattattaat tttcactatt ttgattttgt tttaatgccg attgatcgat cgtcaattca
    26221 acagtagttg ttttttcttt cgagccttca tagtaagtga ttttgacaga atctcccact
    26281 tttttcttat acaaagcaga ttgaagttct acaccagaag aaacttcttt gtcatcgatc
    26341 ttagtaatga cgtcgtattg tttcaaccca gctttttctg ccggtgtagc tgtttggaca
    26401 tttgtcacga tgacaccgtt agttacagat gaaggaatct tcaagatttg ttcttgttgc
    26461 tgagtagaaa cggcagataa gtcgaccatt gtgataccta gagctggacg tgtcactttt
    26521 ccgtcttttt ccagttggtt gatgatattc actacatcgt tacttgggat ggcaaatccc
    26581 attccttcta cgcttacatt agaagtcgat tcagaagtac ttgcgatctt acttgagttg
    26641 ataccgataa cttgtccttc gatattcact aaaggaccac cagaatttcc cgggttgatc
    26701 gctgcatctg tttggatcgc attgatattg actgtttcgt tcgattcatt cgtacttgtt
    26761 acttgtcggt tcaaggaaga aatgattcct gaagtgacag aattcgcata ttcagatcct
    26821 aatggggaac caatcgcaat cgcaggttca ccaaccttca aagcacttga gtccccaaaa
    26881 gaggcaaccg tttcgacttt gtctgaattg atcttcaaca ccgctaaatc tgaataggca
    26941 tctgttccaa ctaattcagc ttttaccttc gtaccgtctt tcatcatgac ttccaaacct
    27001 tgctgtccat ctactacgtg attgtttgtg actacataag cagtattccc gtcttttttg
    27061 tagattacgc cgcttccttc actataagct tccaaattac tatcatctga gctgctactt
    27121 tcttcttgct gcccgaataa tccgccaaaa ccgcttgatt ggttttgact ttgtaagttg
    27181 ataacagaaa cgacagcgtc ttgaacttta tctaccgctt tggtgatatc agaatctaca
    27241 ttgactttga cattttccac aactgtttca ccggcagaat tttgattgcc gcttgtagca
    27301 gtagaactat tgttgccagt gcccatcgct gcataaaaaa tgccagctgt cagtagtcct
    27361 ccaacgatcc ctccaacaag ccccagaccc aacttacgcc agaggctatt atttttattt
    27421 tttttcatct ttggtgtcac attttttcta tccatgtgtt cctcctccaa ttacattcat
    27481 ttatctactg ttagtatagt cttatttgtt gactttagtc tactttgaga ctttgaactt
    27541 tttatgaaaa actttaggaa aaatcattgt tttcagatga aaatatttga ttttcttttt
    27601 ccacagatat tgtgtaaaac tacgcggata ggattggaaa aataagggat aaaacatatc
    27661 cacagcctgt gtataaagtg gataaattct tataaaacgt atgtttgcct aatgaatgtg
    27721 atgaaataag gtaaaacaac gctgttttct gtggataaac ctgtggatgt tgtgcataac
    27781 tgttggggaa atctttttaa caaaaatagt caaaataaac atatagacga aaaaattgat
    27841 tataataaac aaagtgagcg tgaagatcaa aggagagaaa acatgaatat caaaattatt
    27901 tcagtaggaa aattaaaaga aaaatacttg atccaaggta tcaatgagta tgtcaaacgg
    27961 ctgaatgcct atgcgaaaat cgaattgatc gaagttcctg acgaaaaagc cccggagaat
    28021 ttaagcgaag cgcaaatgag acaagtaaaa gaaaaagaag gagaacggat cttagcgaaa
    28081 atcaaagaac aagaatacgt ctatggctta gcaattgaag ggaaaaatcc cacaagcgaa
    28141 tcttttgcta aacaaatcga ccaattaggt atccaaggaa aaagccacct tgtttttgtt
    28201 atcggcggtt ctttaggact cagcgaagcc gtcatgaaaa gaagcaatgc tcagatctct
    28261 tttggcaaaa tgacgtatcc acatcaattg atgcgcctga tcctcgtcga gcaaatctac
    28321 cgtgccttcc ggatcaacgc gggggctcca taccacaaat aatgatattt tttaagagaa
    28381 aaatagcata aaaatctagt tatccgcata aaaactggac ttatcacact ttatcaaggt
    28441 caaaaccact caatttacta ctaatttact acttatgaat gagctttgat acgacgattt
    28501 atccttgaaa agtgaagata taaagatact tccaataaaa tttgaatatt taataggtag
    28561 acacttcaaa aaatgaggtg tctatttttt tacccgattt tgaaaggaag tgaacttatg
    28621 aaaacaaaaa atcaagaatc aaaaggtcgt tccccactct ttaagaccat caaacattca
    28681 ttcagccaat aaaaaagaaa ggataggtaa aaatatggaa cttaaatttg tgattcccaa
    28741 catggaaaaa acattcggca atttagaatt tgctggcgag gataaagtcg ttcagcgaag
    28801 aatcaacgga cggctaactg tcttatcaag aagctataat ctctattctg atgttcaaag
    28861 agcagatgat attgtggtgg tgcttcctgc tgaagctggc gaaaaacatt tcggctttga
    28921 ggaacgtgtg aagttagtca atccacgtat taccgcagag ggctacaaaa tcggcactcg
    28981 tggttttaca aattaccttt tacatgctga cgacatgata aaagaataaa gaaagagagg
    29041 aaaaatgatg agattagcaa atggcattgt attagataaa gacacgactt ttggagaatt
    29101 gaaattctct gctctacgtc gtgaagtgag aatccaaaat gaagacgggt cggtttcaga
    29161 tgaaatcaag gaacgtacct atgacttaaa atccaaagga caaggacgca tgattcaagt
    29221 aagtattcct gccagcgtgc ctttgaaaga gtttgattat aacgcacggg tggaacttat
    29281 caatcccatt gcggacaccg ttgctactgc cacctatcaa ggagcagatg ttgactggta
    29341 tatcaaggca gacgatattg tgctgacaaa ggattctagt tcattcaaag ctcaaccaca
    29401 agcaaagaaa gaaccgacac aagacaaata gtcgctaggt agaaaggaga ctttttcgca
    29461 tgaaacagcg tggtaaaagg attcgcccat ctggtaaaga tttagtcttt cattttacga
    29521 tagcgtcact cctgcctgtt ttcctgctgg ttgtcggact gtttcatgtg aagacaatcc
    29581 agcagatcaa ctggcaggat tttaacctat cacaagcaga taagattgac attccctatt
    29641 taattatcag tttcagtgtc gcaattctta tctgcttgct ggtagcgttt gtattcaaac
    29701 gggttcgcta tgatacggtt aaacaacttt accaccgtca aaaactggca aagatgatac
    29761 ttgaaaacaa gtggtatgaa tctgaacagg tcaaaacaga gggtttcttt aaagatagtg
    29821 ctggtcgtac aaaggaaaag ataacctact tccctaaaat gtattatcga cttaaaaatg
    29881 gcttgataca gatacgggtg gaaatcacgc tgggaaaata tcaagaccaa ctcttacact
    29941 tggaaaagaa attagagagt ggcttgtact gtgagctgac ggataaagag ttaaaggatt
    30001 cctatgtgga atatactttg ctctatgaca ccatagccag tcgtatttct attgatgaag
    30061 tagaagctaa agatggtaaa cttcgcttaa tgaaaaacgt atggtgggaa tatgataagc
    30121 tccctcatat gttgattgct ggtggtacag gtggcggtaa aacttacttt atactgacac
    30181 tgattgaagc cttgcttcat acagattcaa aactgtatat tcttgacccg aaaaatgctg
    30241 accttgcgga cttaggttct gtgatggcaa atgtctacta tagaaaagaa gacttgcttt
    30301 cttgcattga aacattctat gaagaaatga tgaaacgtag tgaggaaatg aagcagatga
    30361 agaactataa gactggcaaa aattatgctt acttaggtct cccggcacac ttcttaatct
    30421 ttgatgaata cgtcgctttc atggaaatgc tgggaacaaa agaaaacacc gcagttatga
    30481 ataagctgaa acagattgtc atgttaggtc gtcaagctgg cttctttcta atactggctt
    30541 gtcaacgtcc agacgcaaaa tatttaggcg acggaatccg tgatca
//
LOCUS       DAFEPQ010000026        30463 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.026, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000026
VERSION     DAFEPQ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.026"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(<1..552)
                     /locus_tag="LP543_001331"
     CDS             complement(<1..552)
                     /locus_tag="LP543_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBL2321505.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(678..2606)
                     /locus_tag="LP543_001332"
     CDS             complement(678..2606)
                     /locus_tag="LP543_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBL2321506.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(2912..3628)
                     /locus_tag="LP543_001333"
     CDS             complement(2912..3628)
                     /locus_tag="LP543_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBL2321507.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(3629..5452)
                     /gene="glpO"
                     /locus_tag="LP543_001334"
     CDS             complement(3629..5452)
                     /gene="glpO"
                     /locus_tag="LP543_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBL2321508.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5466..6962)
                     /gene="glpK"
                     /locus_tag="LP543_001335"
     CDS             complement(5466..6962)
                     /gene="glpK"
                     /locus_tag="LP543_001335"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBL2321509.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7098..8558)
                     /locus_tag="LP543_001336"
     CDS             complement(7098..8558)
                     /locus_tag="LP543_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2321510.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSSFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(8548..9843)
                     /locus_tag="LP543_001337"
     CDS             complement(8548..9843)
                     /locus_tag="LP543_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL2321511.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10175..10852)
                     /locus_tag="LP543_001338"
     CDS             complement(10175..10852)
                     /locus_tag="LP543_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBL2321512.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(10882..11973)
                     /locus_tag="LP543_001339"
     CDS             complement(10882..11973)
                     /locus_tag="LP543_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBL2321513.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKCPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12074..12508)
                     /locus_tag="LP543_001340"
     CDS             complement(12074..12508)
                     /locus_tag="LP543_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL2321514.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13092..14549)
                     /gene="ascB"
                     /locus_tag="LP543_001341"
     CDS             complement(13092..14549)
                     /gene="ascB"
                     /locus_tag="LP543_001341"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL2321515.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(14546..16441)
                     /locus_tag="LP543_001342"
     CDS             complement(14546..16441)
                     /locus_tag="LP543_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL2321516.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(16499..18274)
                     /locus_tag="LP543_001343"
     CDS             complement(16499..18274)
                     /locus_tag="LP543_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBL2321517.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18324..19820)
                     /locus_tag="LP543_001344"
     CDS             complement(18324..19820)
                     /locus_tag="LP543_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL2321518.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20014..21636)
                     /locus_tag="LP543_001345"
     CDS             complement(20014..21636)
                     /locus_tag="LP543_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBL2321519.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(21964..23277)
                     /locus_tag="LP543_001346"
     CDS             complement(21964..23277)
                     /locus_tag="LP543_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBL2321520.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(23522..23746)
                     /locus_tag="LP543_001347"
     CDS             complement(23522..23746)
                     /locus_tag="LP543_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321521.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(23766..24188)
                     /locus_tag="LP543_001348"
     CDS             complement(23766..24188)
                     /locus_tag="LP543_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBL2321522.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(24330..25040)
                     /locus_tag="LP543_001349"
     CDS             complement(24330..25040)
                     /locus_tag="LP543_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321523.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25058..25555)
                     /locus_tag="LP543_001350"
     CDS             complement(25058..25555)
                     /locus_tag="LP543_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2321524.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(25592..26044)
                     /locus_tag="LP543_001351"
     CDS             complement(25592..26044)
                     /locus_tag="LP543_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL2321525.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26179..26784)
                     /locus_tag="LP543_001352"
     CDS             complement(26179..26784)
                     /locus_tag="LP543_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL2321526.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27234..27854)
                     /locus_tag="LP543_001353"
     CDS             complement(27234..27854)
                     /locus_tag="LP543_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBL2321527.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(27856..28725)
                     /locus_tag="LP543_001354"
     CDS             complement(27856..28725)
                     /locus_tag="LP543_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBL2321528.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(28748..29155)
                     /locus_tag="LP543_001355"
     CDS             complement(28748..29155)
                     /locus_tag="LP543_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBL2321529.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29174..30463)
                     /locus_tag="LP543_001356"
     CDS             complement(29174..30463)
                     /locus_tag="LP543_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2321530.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT         8631 a         6627 c         5020 g        10185 t
ORIGIN      
        1 ttttctttga aacatctttc ggtactcgac agcatacaag acaaaagatt gtaaaagacc
       61 gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt ggtaattctg ggaaaagcag
      121 caaaaggatc atgccaacat agatgacagc agtcgctact ttgccatacc acttcgctcc
      181 acttaatttg atatttttag atagcccgat taatccagct acaaacataa agccttcttt
      241 tatcaagaat agaccaaaca acagccaaag ccacggtcga taccatgcca tagacaaaat
      301 cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc accttcccta agtctgttac
      361 ctgattatat ctccgagcaa tgaccccgtc tagataatca gacaaacctg agacaacaat
      421 gaccgctcct gctatataaa atgactgtct cagcacaaaa taaagataaa tcggcagcaa
      481 aaaaatacgg aatatcgata aaagattcgg aatcgtttgt atttctttac gccagtctgt
      541 gcttaatttc attttatcgc cctacctcct cagcactctt ttctttttat tttagcttac
      601 acggggaata aaaccaatga tttcactgtt tttatttttc tttacagcca ctgatattat
      661 gagctaattt gagataatca cttgctgttt tcatcgttta ctcttctttc ttcctgtcgt
      721 ttgcgcattt cttttactag tactttatga gctttttttg atcctcgctc taggtcacgc
      781 tgccgtttac gtgttttata atcgataaac agactgtcta aatcgtaacc tgtcgggtac
      841 agatcttctg ctgaacgcac cagacgaaca cgctgacgag gcacgtggat tatttttttg
      901 tctaaatata catcaatcgt atcttcacct atatcttcat aaactaacgc ttctttttgg
      961 tattcattga caaatatccg atcacctttt gcaaagagct ggcgttcttt tttattctcc
     1021 ttcctatttg taactgctgt cttaggaaga aattcttttt tctgtgtccg ataggctttt
     1081 tccgccaagt aacgctctgc ttgatggatc aatttcatcg acatatccat tttatgagca
     1141 atccacaaag cttggctttc tcctgtttct ccaacccgca actgatattt aggacgaagt
     1201 gcttcccgat cgaaagccat agcagccggc acaaaatctt catgatctcc ggcgaatttc
     1261 ttgatttctc cataatgggt cgtggcaatg atcaatgccc cttgctcata catagattcc
     1321 atgattgcga tcgctagtgc agccccttca tttggctctg ttccactgcc aatctcatct
     1381 aaaagtacca acgtattcct ttttatcttt ttcaaaatag cagcgatgtt ttgcatatga
     1441 ccagaaaatg tactcaacgc attttctaaa ttttggtggt caccgacatc gacgaaaatc
     1501 tcatcaaaga cagcaagctc tgtcccttct tttgccggaa cttgcatacc gaacatcgcc
     1561 atcaaagtca ataaaccgac tgtcttcaac acgactgttt tgcctccggc atttgctccg
     1621 gtgataatca agcctcgata gtctttaccg atttcaacat ccaatggtac cgcatgctca
     1681 ggtaaaaatg gatgtctgcc ttgcttgata atgatatgtt ccgatttatt gattctaggt
     1741 gtgcttccat tgatttctcg gctaaatttt gcccgagcaa agatgatatc taacaccgtg
     1801 atcgtttcga tcaataaatc gatcacttct tcgttttctg cgatagcacc agtcaatgcc
     1861 gccaggactt gatattcttc actgatttct tcagctttta acaactgata gtgttcattc
     1921 aatttagaaa tcacagttgg ttcaataaaa accgtcgttc ttttatttga ttcatcgata
     1981 atcgttccat ctattttatt tttatagctg gctttgatag gaatcgtata gtattcgcct
     2041 ttttgaacaa tcattgcttc ttggatcatt tctttgtttt tcggatgacg caagaatttc
     2101 agtaattttg actggatttc tttttcgatt agttgtaatt gtttgcgtac tttacgcaaa
     2161 ttacgtgacg cgtcgtcact gactttatta ttttttattt tctggtcaat caactcttct
     2221 acatttatca aatcaggaag atgtttgcta taagcaaaca acaagggtgt ctggtattga
     2281 ttcttatcaa aaaactttgt gatcatccga ctgcttctaa gaaaatccgc atattcgatc
     2341 aaatcggttg gctgcagaat caaccctttt ttgacttgtt ctgtcagtgc atcgatcctc
     2401 ggcaacccca taaatggaac atgctgactg ctttctaaga tcaagcgtgc ttctcttgtt
     2461 tcttcttgac gatccaagac agtttgtagg tttgtcgaga ccgtcatatc gctgattcgt
     2521 tttttgctat agtttccaat tgctcttgct tggatttcct ttttgatccg atcaaattgt
     2581 aattgactaa tcatttcgtt gtccatattc tctccttttc ttgaagagat acctgtccca
     2641 aaataaataa gggcctagag ataaataaaa ggatagaaca cagcttaaaa aagtcatgtc
     2701 ctatccttat tagaatacaa agatattgtt ttcctgacag gtaccgtttc ataagcacac
     2761 caaataaact ttgtttattg gctattcgac ttactgatta gataccagct acactcacaa
     2821 aacatacccc attcttctag gtcttattta ttttccactt aactatagca agacacattt
     2881 tggttgtcaa actttagtat gttttataga tttacaccat taatgaatag acacctaccg
     2941 cgataattgc tccgatcatt ggacctgcga ttggtaccca tgaatatgcc caatctgaag
     3001 ttcctttatt agctattggc aataccgcat gtgctaatcg aggaccaaag tcccgagcag
     3061 ggttgattgc atatcctgtt ggccctccta atgaaagacc gatggctaag atcaatccac
     3121 ctacagcgaa tacgttcgtt ccaggtgcaa attcgttttg tccaaaagct agcaacccaa
     3181 gaactaatac aaatgtacca attaattctg tgatcacatt tgctggataa ttccgtatag
     3241 caggacctgt tgcaaacgtt cctaaaatcg cgctttcatc tttcgttgca ttccaatgtg
     3301 gcaaataaga aagccaaaca agaatggccc ctgcaaaagc tcctaaaact tgcgcaataa
     3361 tgaaaggaac gacaagattc caactaattg cccctgtcat cgccatagct agagagactg
     3421 caggattcaa atgagctggg cccatgaatc cagaaatata gacagccatt gttacagcga
     3481 gtccccaacc aaatgcgatg acaatccagc cagaagcctg tgctttactt ttgtttaaat
     3541 tcacagccgc acacacacca tctccaagca aaactaggat cagtgtccca ataaactccc
     3601 ccatgatttg agtcatatca ctgttcattt atttctcccc ctttagttcc cgcagatctg
     3661 attctgcaat caatgcttct aaacgtttag tctgttgcgc cttctcctct tctgtccatt
     3721 caaaatatcc agcaattgca tcgatcacag gttgcttgat ctcatcaagc tgatctcgtt
     3781 caaacaacag atgatttgta cgacgaatga gataatcgcc tggggccaaa accatttctt
     3841 cttctaaacc ataacgtaac cgagctgact cagcgagact caaaccagga taaggtgcca
     3901 tttcttttgc taattcaaag atacgtcggg cattagtccc gtaaaaatca gcgatataag
     3961 tagcatcttc ttcctctaaa ccggctgcta ctccttcttt tgctaattct gtcactgttt
     4021 cttctaattt agttggatcg aagtttccac ctgaaatctg atatttttta gagtcgattt
     4081 ctttcgtctc tattccgtat tcttctttta ataattggcg tatcaatcgc aaagctcctt
     4141 ctgccatctt acggtaatcg gtgattttcc caccagataa agtcagcaaa ccatctggtt
     4201 ctctttctaa agaactacct ctggaaatcg tagaaggtgc tttttcatca cggctgtttt
     4261 ccaaatggtt caacacgtct tctacttcag cacgagaaac tttgttttcc ttatattcac
     4321 ttactgtatc gacaacttta ttgaagctct tgtctgaaat cgatccatta tctccaccat
     4381 tgtaatcaga accagaatta ccaatcaaca gtggacgaag tcctgcccag cttgcttcga
     4441 tatcattcaa tgtgatattt gcttctggat agcgatgatt gatcacatcc aatagataat
     4501 ctacatcttc ttgtgtgact ttcggatccg taaaatctcc ttggtaatcc gtatctgtcg
     4561 taccaaagta agtcttgttt tctcttggaa tagcaaaaac catccgccca tcttgctttc
     4621 ctgtatcgaa gtatgtgggt tgcggtacag gcagtttttt cgcatctaca actaaatgga
     4681 tccctttagt tggacgcatt ttaggagaga ctggacgcgt aaaattcaag tttcgtactt
     4741 tatctaccca aggaccactc gtattgatca ctaattttgc tttgatttcg atcacttcat
     4801 ctgtcagcag gtcacgagct ttgacgccaa cgatttgatc gccatcatat aaaaagccaa
     4861 ccgctttcat tttacttact agataagccc catcttcggc agcctttttg atattatcaa
     4921 tcactaaacg ggcatcattg ttgcggaaat ctagatagac acccgcgcct tttagccctt
     4981 cttttttcaa aaacggttct ctttccaata cttcttctgg tgtcagcgta taattctcat
     5041 atttggttcc tgtcacattt gccagtttgt cataaaggtc cattgctact ttgaccgaga
     5101 acatattgaa ggttgttgct ccttcgccct catagattgg caaaagcatt ggatctggtt
     5161 ttgggatgtg tggggcaata ctttgtacga ctgcacgttc accaactgtg tcagctacta
     5221 cttccacatc aaatgtcttc agataacgaa taccgccatg aacaagtttg gtcgagcgag
     5281 aggaagtccc ttctgcaaaa tcctgcattt cgatcaatcc tgttttgatt cctgatgctg
     5341 ctgactggac tgccacaccg gcaccagtga ttccgccacc gatgatcaac agatccaatt
     5401 cttcttgctg cattttttga atgctatctt gtcttgtctt gtttgaaaac atatggattt
     5461 cctccttatt caaatgcttg ggtagctttt actgcttttt tccaaccgtt gtaaagtttt
     5521 tctcggcgct cttcctccat ttgaacttca aatagcttgc cagcttcata aaattcgcgg
     5581 atctcgtctg tatctttcca ataaccgaca gctaatcctg ctaaaaatgc tgcccctagc
     5641 gccgttgttt ccaaattttc tgctcgttgg atcggtacat tcaagatatc tgtttggaat
     5701 tgcatcagat attcattgtt tgctgcaccg ccatctactt tcaatacagg aatcttgatt
     5761 ccagtgtcat cttgcatcgt atcgatgata tctctcactt gataagcaat cgactgtaaa
     5821 gtagctttaa tgatatcttc ttttgtcgta ccacgtgtca agccaaacat agcccctcgt
     5881 gctgcctgat cccaataagg agcacctagc ccaacgaatg caggaacaac ataaacctca
     5941 tcttcgcttg tagagcgttt cgctgcgtct tctgaatcgc tagctttttg caacatttga
     6001 agtccatctc tcagccattg aatcgaagaa ccagccacaa aaatacttcc ttctaaagca
     6061 tagtaaacct ttccatttat gccgtaacca attgtcgtca ataaattatt tttggataat
     6121 tgcggttttt caccggtatt catgacaata aatgaacctg taccataagt attcttgacc
     6181 atgcctggtt cgaatgccat ttgtccgaat aatgcggctt gctggtctcc tgccatccct
     6241 gaaattggtg cttcgcttcc ataaaaatga taaccttgcg tataaccata aacttctgag
     6301 ttagaagtga cttttggtaa catcactcga ggaatattta gtaaatctaa aatctcctga
     6361 tcccaatcta gctgatggat attgaacaac atcgtccgac tggcattcga gtaatcagta
     6421 acatgtgctt gtccatcggt cagtttccat actaaccaag aatcgatcgt tccaaacatc
     6481 aattcgcctt tttcagctcg ttcttgcgcg ccttctacat gatcaagaat ccagcgtatc
     6541 ttcgttgctg agaaataggc atcgatcact aaccctgttt tttcgtggaa aaattcttga
     6601 tagccttcat tttttaagtt atctgcaatt ccagctgact gtctggattg ccaaacaact
     6661 gcatgataga ttggacgtcc tgtatctttt tcccatatta ctgttgtttc acgctggttc
     6721 gttatgccaa tcccagcaat ctgatttggt ttgatccctg attcgatgaa cgctccggca
     6781 ataactgact gtacagaatt ccagatttca tttgcatcat gttccaccca actttcttgc
     6841 gggaaatatt gcgtgaactc tttttgagaa ctgccgatgt gtcttccttt cttgtcaaag
     6901 ataattgctc gtgagctagt tgtaccttgg tcaatcgcca tgatatacgt tgattctgtc
     6961 attgtgattc cctccattat atgcaaacgc tttctttaac tactcttatc atactactgt
     7021 tttaaaaaaa gaaactgtca aaattttatt ctttttttct ttttttgaca gttttttatt
     7081 atgaagctat tttttaatca attcttttct tttttcagcg tctctattct tcttttcaat
     7141 cgaataatgt cgtaaggtga cgcaaattct gacagataat attcacactg ttttttgttt
     7201 ctctggcaaa atgtgatcac caaatcatat tctttacctt cttcataact tccaatctca
     7261 ataccgccga ttcccctcag ttctctgatc agatattgtt gaaaagcaat acgataaatc
     7321 ggcagacttt ctaaatcgat tccaatcact actggttttg caatataaaa atcgatcatc
     7381 aaaagaacat tggcatactg gatcatcagg taactaagct cactatcttc agctcttttc
     7441 ctcggcaatt gctgcaccgc tgtttcctgt aatttcgcca gtaactgtgt taaggaatgc
     7501 ccgagcattt tctgctgctg ttccaaaagc cgatcacgct catagacttc gatctttcca
     7561 acaaagaaaa accattcatt gttgatctgg gaaatttggt agcctactgc cttttcttct
     7621 ttgattttca aacgacgagg acgataatgc cataacatcg tttcccgaat gtaagtatct
     7681 aaaacagcgg ttggtaactt tttgctccgt gtcaaatcat actgataaaa tatttcttcg
     7741 tctactatcg aaaaagaaac aaaagagctg taaaacatca ttgtttcgta tacattcagt
     7801 tctgctgctg ttcgactaag atgcatcacg atgattggat cgattgcttg ataaagccgg
     7861 tccgactgat agagtaattt cttttctttc aaagttgcgt acgtactttt ttcattcaat
     7921 agtctttttt tcgtaatccc gagccagcaa gcaatctgtg cttctgctga tgccgtaaac
     7981 gttgtttcta atacacggtc caatcctaga atgaacggtc ttttttctgg tgtattttgc
     8041 aaaaaaagcg gtcgctgatc ttccgggata tattggaata gttcataata aaaatatcgt
     8101 atctgtagct cttctccgta taattgaccg tttttgattt gtaatccaaa ttcctctaag
     8161 agctggttca attctttgat ctttcgaaaa acagaagact cactgatatt aaacgctgtc
     8221 gctaatcgaa cgatggatac ttgctgatgt tctaataaat agacgagcat cttaaacttc
     8281 aagctttcct gcaataaaaa caatagaatt ttttccaaac ttacatcttc gtccaacaca
     8341 agcagcgcct gatactcatt tcgctgtact tctacttttt tttccatcat tcggccaatc
     8401 aattcaatat cttccaaata ttgatcaaac gaggcttttg acagttggac atgctctctc
     8461 aaaccattga tagaggctgt tcccccttgc agaacaaggt ttcgcaaaat caggatctgt
     8521 aactgctctt ttctttctaa taatgcttca atcttcatag gcagtattcg ccacctttga
     8581 ccacaccata tctggcacac ggtattctgg ctggaaaaat agctcatgtt cttctaactg
     8641 ttcaaacgtc catccttctt ttactgcaat tgtcagttgg tcaagaaggt ggaacatctc
     8701 ttttttgctt tggagctgtg ctccaagaat ctttttttca gaatcaaata gaaatttgag
     8761 tttaactttg ctttgattta ttgctgtaag cttgtagtct acgtatcctg tttcaatttt
     8821 ttggggataa aaaatgctct cttcttctgt caaacctacg cttcctaaga aatagccaaa
     8881 ccactgattg ccgactggac gataggttcc ctgatccttt gtctgtgagc cactaattgt
     8941 cttgctgaca tttcgtgctg tacggacagc gttatttact agagaaacat acattttttc
     9001 atttgttgga cgaaaagtta cttggatcgc atcgccaatc gcataaacgt ctttttgaga
     9061 cgtttgcaag tagtcatcta cccagatcgt tccatcatca ttcaatgtta attgttcttc
     9121 ccacatctga ttgtcggggc gagaatgatt agccagcaaa acaagatcat agttctcctt
     9181 tcttttttcc gtctctacaa tagcggtttc attttcggtc aaagacacaa cttgttcttc
     9241 taaaaaaagt gaaagccctt gatttttcat ttcctttact aacggttctg tcatttcagg
     9301 atctagataa cgaaaaagaa ttgactttcg gctttcgtaa agatggattt gtttttttct
     9361 tgatgccaac ccttcagcta tttcgattcc tgcttgtcct gcaccaataa ttgcaatttt
     9421 ctttgcattc atgatctttt gctgaagagc cgttacgttt tgtatctttt taactgggtg
     9481 aataagcgtt gattctgcag aaatatttcg aaacttttga tttgaaccag tcgctagaat
     9541 caaacgatca aaatccaatt tttttcctcc tgctaaaacg actttttttt ctttcaatcc
     9601 tattactgtc ttttctgtgt aaacatggat tcgatagcgg tcagtcagtt cttctttcgt
     9661 gatccaagaa agttgttttt cactggtaac ctttcctttt aatatcaaat tgaacccgct
     9721 gggaatccat cctaacttcg attgcttttc aatcaaatat atttcacttg ttggcatcaa
     9781 ttggcgaaga taaatagccg catgaatccc tgcaaacgac ccgccgacaa ttacaatgtt
     9841 catctgcatt cctctttctt ataaggtgtt ctactgctaa cttctcaatc agaaaacaca
     9901 gtattcataa ctcgctttca accttatgat acatagcgat gcagcagaat caaccctctc
     9961 catcataccg tgttctaggt ctgcttctca ataatgtact agtggaccta attgaagagt
    10021 atcatttctg aagagatgtt tcaaaaaatg tactcttata aaagaaaaaa actcgccttt
    10081 tacgtacctc tgtccaaata ggaaacagaa aagaacgaaa agacgagttt tagcgtgtgt
    10141 aatgtaatag ttagaataga cacatcattt atttttaatt ctttaatgct tcaacaactg
    10201 cttcgcctaa ttgatgagca gattgaggat tttgccctgt aagaagccgc ccatcaattt
    10261 gaaccacttc tgaaaaagct gctccagact gatagtttgc tcctttttct ttcaaagcat
    10321 cttctgctaa gaatggtact tcattcgttg taccatttgc ttcctcttct tcattactga
    10381 acccagtcac tgtacgtcca gctaaaatag attttccaga ctcatctgta actggcaaca
    10441 atcctacaac accgtgacaa acagctgaga ccagcccttc atttcgatag atttgtgaag
    10501 cgactttccc aagatctatt gcttctggaa aatcccacat tgttccgtgg ccaccagcat
    10561 aataaaccgc tgcataatca tttgcatcca catcatcagg acgtaaggta tttcccaagg
    10621 cccgatttct gaaatcttca tcttcataat aggcccaatc gatttcatcc atattcaaaa
    10681 cactttgcgg ttctaaaggt acatatccgc ctttaggact gacaaaatct gcatcgatcc
    10741 cattttttac taatacatca taaaaatgag tcagttcact taaccacaaa cctgtaggac
    10801 gctcgatctt ttcgtacttt tctacatttg ttaatacgat caatactttc attttcccat
    10861 cctctttcta taaaaattgc gttattgata acgtgctttc aagaaattat ataatgggct
    10921 tggcaaataa agctctttat atgcttcttc aaaaggtttc gttacaaatt catcctcacg
    10981 gttttccaat aatttctctg cccagtcagg atcgacgatc atttgctgtc caattgctgc
    11041 aagttctgca tatttcaata cctgctctac atcttcgcga ttgcggatcc caccgacacc
    11101 aactaaaggg atctttcctt gaatcgtgtt atggaccact tcaagaatcg atttatcgtt
    11161 gtatttatca gaacgagcta ttcgatcata catatttaat gaaacatgca gataatctag
    11221 ttttgactca cgtaatcgtt caagaagcca gatcgtgtct tcaaaacgga tacctggtgt
    11281 ttcatattct tctggtgaga aacgataccc tacgatgaat ggttttttag catattgttc
    11341 aaccgctgag aaaaccgttg ttaataattc ttcaataaaa cgataacgat tttccaagct
    11401 accgccccat tgatcctctc gtctattcga atgcggagag aaaaattgtt ggatcaaata
    11461 cgtatttgct ccatgcaact cgattccatc aaatcctgat tcaatcgctc tacgtgtggc
    11521 tttgccaaat gcttggattg tttcaacgat ttcttcttca ctcattgctc gtggtgtttc
    11581 cgcatcagga cattttgctg caactgcact tgcagataca ggctgttctc cagataaagt
    11641 tgctcgactt gtcattcgtc caccgtggaa aatctgaata atcgcttttg cgccttctcc
    11701 ttggattgct ttcgctagct cacttaatcg aggaatcatt tcatcatgag cgatgctgag
    11761 ttcacctggc cagccttttc cgatatcttg gacatttgca gcaccagtaa taacagcacc
    11821 aagaccttta gaacgttgtt tataataagt gatctcatct tcggtgatca ctccattgaa
    11881 aaagctgagt tgtgtcgtca taggcgacag catcaatcgg ttcttcaatt gaactcctga
    11941 aggtaaagta attgattgat caaattgtga cattgtcatt cctcccataa ttcgctctga
    12001 ttaacctatc gagaatcatc tatcattaaa ccactcgatt agttgtacat gcaactaatt
    12061 tgcttaaaaa attttatttt ttttgtaact tatctgttgc atcattcatt gtctgggcta
    12121 attctttact tgcttcattt cccaaaactg ctttgtatga ctgatgaagt tcttcccagc
    12181 taccatcgac ttccttagca aattggcgtc cttgttctgt aagataaact aatgacaagc
    12241 gaccttcgct aactgttttg acgtatcctt tatattctaa tttttcaaca aaacgagcaa
    12301 tcgttgatgg ggcggaatag agcatttgtg caatatcttt ttgtggtatt ccatcttttt
    12361 cttttaatat caataaaata aaaccataag ttggtgccag acctgtcctt ctaaacgctt
    12421 catcagctaa tttattcaac atgcgatcta gctttttcac gctaaagaaa agacattcat
    12481 ctaacgcttt ttcaattggc ttttccattt catcccctcc attggcgtac atatcatagt
    12541 atgattttta ttttttgtca aaaaacacgg aataatgtcc gcctcctctg tctagatttg
    12601 aaaaaaagaa gtagccatct tcaaagaatt tcggcttgtt ttcgacaaaa ctccttctgg
    12661 aacaccgtga atcagaggtt gtgaaaaagt cgtttagttt tgagtatgaa gaaaaatttt
    12721 gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgaaaa actggctttt
    12781 gaacaccgtt tatatcagag gttgtgaaaa agccgtcctg catcaagtaa taagaacagc
    12841 caaacaaaaa tagttcctca tattttgtcg ttttttgagc taatgccgat ggtcagctga
    12901 ttgtacaccg tttattgaga ggtcgtgagc ctgccgttga actccgagtc acaaggagca
    12961 atttcaaaaa acagcttctc atgttttatg actatggtga cttgtgatcg aggagttggc
    13021 aggagaatac tgtttattcg taaagaaggg gctgtgacaa gacttttgtc acagtccctt
    13081 atttcctttt attataggat ttcgccattt gattggatga cttgtctgta ccaatcaaat
    13141 gattttttct ttaaccgttt ctttgtgcca ttcccttcgt catcttgatc aacatagata
    13201 tatccataac gttttgacat ttgtgaagta gaagcgctga cgatatcaat gcagccccac
    13261 gacgtataac caaataaatc tactccatct ttgatagctt ccttcatctg ctcaatatgc
    13321 tgcttcaaat agtcaatgcg gtaatcgtct tgaacggttc catcttcttc tactttgtct
    13381 atagctccga gtccgttttc agctatgaac agtggttttt gatacctatc atataattga
    13441 ttcaaagcaa ttctcaaacc cttaggatca atctgccagc cccattcact tgatggaaga
    13501 tacggatttt taattcctcc agttattaag tttccttgga ccccttccca attttctgga
    13561 tgagcactga ctgcactaga catgtagtag ctaaaagaaa caaaatcaac agggtaggct
    13621 gctaagattt cttcatcacc tacttctatt tttaagttga cacctttttc ttcaaacatt
    13681 cgttgcgtat acgctggata atatcctctt gcttgaacat cactaaagaa caaagaagtt
    13741 ctttgctgct caattgtttt ttgaatatca tccggatcac aagaatacgg aacaagaagc
    13801 tgatagctga tcatacaacc gacttgtgaa cctttgataa tttcatgaca agcttttgta
    13861 gctaaagcac ttgctataaa ctgatggtgt gcaccttggt atttcgcttg ttccaaatgg
    13921 ggatggtttt cttcaattac tcctacgcta acaaaagggt gatgttttac acagttgatc
    13981 tcattgaaag tcatccaata ttttacttta tttttatagc gtttgaatac tgtttcagcg
    14041 aaacgaacgt aaaactgaat aagctttcga ttaggccatc caccatattc tgtaaccaaa
    14101 tataacggca tttcataatg ggaaagagaa acgactggct caatcccata ttttagacat
    14161 tcgtcaaaca cttgatcata aaaaaataat cccgcttcat tgggtgtttc ttcatcacca
    14221 tgaggaaaaa ttcttgtcca tgcgatagac atccgatagc atttaaaacc catttcagca
    14281 aataaacgga tgtcttcttt aaagtgatga tagaaatcat ttccgtgtct tttaggataa
    14341 taatacttgc tgttgggatc ctgcgccgct tttacttgct gatgcgtcat catatgccat
    14401 tgatctgtcc atttttcttt aggtaaatcg ctgtcgaatg tatacacatc agacacagat
    14461 aatccttttt catctacatc ccatgcacct tcacattgat tggccgctgt cgctccgccc
    14521 cataaaaatc cttctggaaa ttttttcata tcgatccccc taaacttgct agttttcttg
    14581 tttgattatt tttcttatga ctagtaatga tatttttgaa tctactacta tttgtaacaa
    14641 taaccattgt ggtcggatca tatccctctt tgattaattc ttgcaaatct acttccatca
    14701 ataaatcgcc tttacgtact ttttttccat cagatactgc actttgaaag tatttgccat
    14761 ttaaattcac tgtatcaata ccgatatgga tcagtatctc tgctccttct tcactgacta
    14821 ccccaattgc atgtttcgtt ggaaaaactg ttgaaacttc cccgtttaca gaactgtaga
    14881 tttttccgtc cgttggaatg atcgcaaacc cttctcctaa agatccacta gcaaatgttg
    14941 gatcgtttac ttcagataaa tcaattattt cacctgctgc aggagtactt aacactatat
    15001 cctttgagga ttcttcttcg atttcatcct tgtagccgaa aagaaaagtt agcatacagg
    15061 aactgatcat cgctaagaaa taactagcaa ctagccaaac aaatccttca ccaatataaa
    15121 tcgggaaaga caaaatactt cttgtagcaa ctgccaaagc accggctcct gcggcaccag
    15181 ctactccacc aaatatagct ccacatatac aagcaatgta aaatggtcgt ttatatttaa
    15241 gagtcacacc ataaacagca ggttcagtga ttccaaataa tgctgtgata aatgcagata
    15301 aagataaact ttttactttc ttatttttcg ttttcaaaaa taccccaagt actgctcctg
    15361 cttgcgcagc tacggcagtc atacatgctg gacctagcgt atcacgaccg tagaaagaaa
    15421 gattgttggt cattactggt acaagtcccc aatgtaaccc aaaaatcact aacacttgcc
    15481 aaaatccgcc gataacagca ccagataaaa cagggctaaa gttataaaca gctaaatatc
    15541 cagctgcaat cagctgactt gcataatctg caattggacc tacaactaat aaaataattg
    15601 ggaccatcac gatgatacaa atgaatggtg tcaataaatt tcgaatactt tcgtgcaagc
    15661 ttttgcgaat cttaggctca atataagaaa gaacccatac agctaaaatt gctggaataa
    15721 ccgtacttgt ataccttgct agaatcacgg gcatccctag aaatcgcaag gttatgctat
    15781 cactatatgc actgacaatc gtaggataaa cgagagctgc tgcaataacc atagcgatga
    15841 aacgatctgt attgaacttt tttgcagcag tatatgccaa aaatataggc aagaaataga
    15901 aaaatgcatc agctgctgca ttcaaaatga tataagttcc tgattcagta gttaataaat
    15961 tcaaactcgt acataaggcg agtactcctt tgatcaaacc acttcctgcc aagacgccta
    16021 aagtcggggc aaaaacagcg gcgatcatgt ctataaaaga gtttaaaatg ccttttttct
    16081 cagttcctct ctttccacta gatgcatcat cttctagttt catttgtttg gataatacgt
    16141 catacacttc atttactgca tttcctacta cgacttgaaa cattcctcca gcttcgacta
    16201 cagtgatcac accttctagt tgctctacct tctccttatc tgccttctta ttatctttta
    16261 gggtaaagcg taaccttgtt gcacaatggt aaacattgct gacattttct tcgcctccga
    16321 caaatcggac aatatctttc gctaattgtg aatagtccat ttttacttcc cccttgttaa
    16381 ttaatgccgt agcaatcctt cttacaagat aagtatagtt cttagaaaac ggtttctcca
    16441 ttcaattagt accccttcta atagcgaacg aaactgtaca atagtgtgta tggtttactt
    16501 agccgagcaa atcactcact gcttgaaaga aaacaagtat atcgtttctc ttatagtcaa
    16561 aggagacaaa aaaaacacgc ttgatcttcc tttgctgatg tataagtggt atctcaagct
    16621 gatactctgt gatttttgtt tctacttggc ttttgataag acagcacaac aaagtctcgg
    16681 catttaacgg aagcataact aggtcagaac taggagagat tagttgctga atggaatcta
    16741 tctttttatg gatttttatt tgattttctt ctttgtagta atgatcaagc agttttgttt
    16801 tctttttttc ttcaactatg gtcagctttt cacgcaaaat ctgataaatc tcccaagatt
    16861 caaactcatc tagaacacta ggtagataaa tcaaatcatc ccactcaaaa ataagctctt
    16921 gctcagtagt caaaattgca tcatattccg ttattttgtt tgattgattt ttgaaaagat
    16981 acactggttc tatccggcat tctttgatcc acatgttcat actttgttga atctgttttt
    17041 ttagtgtatt caaaatacta attggctggt tactaacaat cagtatctcc ccttgttttc
    17101 gaaaaggatt caacgcttcg gcaagataca aaacaaaata agcgctttca gctaaattgg
    17161 gacgtaattg aaaatgtatt gggaagaaac gccgaattaa ataaatttct aaaggataac
    17221 gggagatgat cttttgtaca aaatgattca tggtattatg tccggcatcc atcctcgttt
    17281 tcatttgttt gatatgggtt agcaaaaatt ctgtttcatg gaacaataac gaaacaggaa
    17341 gatctaaaaa acgaataatc gtttgctcaa atgctcttag attcttctct atttctttgt
    17401 ttttttcatc attaatcatc aaataattta actctaatag tcgattttca attgcttgtt
    17461 tttcttgatt ggaaaaagaa tagggtagtt ctgatgataa cttttttgtg agggtctcta
    17521 ccatcggaat aaataatgta tacctgtttg ttttttctaa aagttcttct cttcgaagaa
    17581 tggtcaaaac gagataccga gaaaatcgta tgaagtcctc tcctgaaaca atataattat
    17641 atgatataag aacctctttt aatgtctttt ggacagtcgg catgatattg gaaaaaattg
    17701 tactgatttg ttgaggaaac cttgcgtgtt ggatcaccgt ttctgttcca acaagagata
    17761 agaaaattct ttttgaattt tcttcagcaa tgatttttag tccttttgtc gacgaaactt
    17821 ccagttctaa tttaggattg cgctcgatcc aacgcaaaat cgtttttatt tgtaatgata
    17881 cggctgtttt tgaaagaaag aaaatgtctg ctatcttttg catactgaca tacccttttt
    17941 gaaacaataa gtacgtcaca atagttgaag ctcggaaatc catgctggaa tagattttat
    18001 gtttcgtcac ctcttgtatg atttgctgtt tagtttcaga ttcaattgat tctaaaaaat
    18061 aacctgaacg tttatttgac tggataatga ttttcttctc gtagatagca agcatatttt
    18121 tgatttcagt ttggatcgtt ttcttgttgg tacctaaagc ttcagctaat tcttcgcttg
    18181 ttatccaatg attcttattt cttaatacta caagaatttg gagttgtcgt ttatttaagt
    18241 ccatttgctt acacctcctc tttgttataa acatacagga aaaacctctc aaatgacaat
    18301 agtcacttgc agaggtttaa aaatcatttg aagaaagatc tatattcaag gtttttacca
    18361 ttagactgga taatttgttt ataatattca aaactatctt ttttcaatct cctaaagctt
    18421 ccgctcccat cattttgtct atccacataa ataaagccat atcgcttcct catttcccct
    18481 gttcctgccg atacgatatc gattggtccc caccataagt aaccaatgat ttggcaacca
    18541 tcttttattg cctcagcaat ttcctgaaga tgtattttta gatactcaca acgttttgga
    18601 tcgtggattc gattcaattc atctggtgct tcatctagtc caatgccatt ttcaactatg
    18661 aataatggta attcataacg gtctgtcaat tcattcatga cataacgaaa ccctagagga
    18721 tcaattggcc aactccaagg ttcgggagat ttttccttca aaaagggatt atcgaaacca
    18781 tttgctccgc cagtatctac gcgcatttct gcttcttttt tatagacagc gctacgataa
    18841 taactaaatg caatataatc tgccgtgtta gcttgcaagc actgttcgtc tccatctaac
    18901 attactggtg cacagtcttc ttccttccat gtcctgtaaa tataagctgg ataatgccct
    18961 cggcacatga catcggagaa aaaccatgaa ttgcgtttaa attgcagtga accaaataca
    19021 ttctctggat cacagtcata tgcataagtc gctaatgcag aaaagctaca cataataccc
    19081 atcttagcat ttggatcgat tttctttaat aattgagtag cacgggcgtt cgctagaaac
    19141 atatgatgac aggcttgata aatcactttt tttgttagtg tagcaatggg ttcaagttta
    19201 tctctagcag ggaatgtaac caatccagca gctgcataag gcatacgaaa agcattattg
    19261 atctcattaa aggtcatcca atatttcact ttcccttggt agcgttcaaa gacggtctgc
    19321 gcataaaatt caaaaaagtc gattaatttt cgattttgcc accccccata ctctcctacc
    19381 aatgctaagg gggtttcata atgactaaga gtaattactg gctcgatatc atatcgattt
    19441 aatgtattga ttagtctgtc ataaaatacg agaccagcct catttggctt ctgttcgtct
    19501 cctcttggaa atattcgtgc ccatgagatg cttgttcgaa aagcttttaa acctaactcc
    19561 ttcagtaaac gaatatcctc ttcaaatcga tgataaaaat caatcgcttc atgagaaaga
    19621 taatggagtg attcgtctaa agcaatctcc caaatctttt tttcttcatt ccactgaata
    19681 ctaggatgct ggttcatgat cccgataagc gtatctgtca cattaggtaa tttgccgtct
    19741 tccaaccatg caccttcaca ttgattagca gctactgctc cgccccataa aaaattttct
    19801 ggaaatattt cctgatccat aactatctct cctcgctatc aacaatttct tacttgaagt
    19861 atagctcgat tcatcaaaac aagatattct aactatttcg gcagaagaga agaaaaaaat
    19921 tgaatgctac tcttcatttt taatatttat atataacaaa tacataagac gatccccgaa
    19981 agaaaacact cgttaaccat ctaagcaaac gatttatttt acttccaaca caaatgcttc
    20041 ataaggtcgt aaatcaactt tgccgtctgg taactgtttc agaggataat ttgacacaat
    20101 aacccttaaa gcttcacgtg gaatagtgaa cgtctgcttt tcactggaca tattacaaat
    20161 aacgagccat tgctggtctt catatatgcg agaataggca aagacatgct caggcgtctt
    20221 ttccactaat tcatagtctc cccagacgat gatgtcatgt tttctacgca aatcgatcaa
    20281 tttcttgtac atataaaaaa cagaatcttg atctgctaac gcttcttctg cattgatttc
    20341 tttataattt ggattgactc gaagccatgg aattccagta gtaaacccag catgttcacg
    20401 ggcgttccat tgcataggcg tcctagcatt gtctcttcct ttcgccttga ttgattccaa
    20461 gatttcctct tttgtaaatc cagaagaaat cctttcgtta tacatattga ttgtctcaat
    20521 atcttttgct tccgcgatat catcgattgg gcaattcgtc atgcctaact cttctccttg
    20581 atagatataa ggggtgcctt tcatcatatg gagcaagata gcaaacatct tggcactttc
    20641 ttttcgatac acatcgtcat ttccccaacg agaaacaatt cgcggaagat cgtgattatt
    20701 ccaaaataaa ctattccatc cttcatcacc taaagatgtt tgccatttgg ataatacttt
    20761 tttcagttca gagatactca agggtttcaa atcccatttg tctttgcctt cttgctgatc
    20821 taatcctatg tgttcgaatt ggaagaccat ggataattca tttcttttag gattggaata
    20881 aagtttagcg atttcggtag ttgctcccca tgtttcgcca acagtcatca gatttttgtc
    20941 accaaaagtt gcttgattca tttcctgaag atactcatgc agtttaggtc cattacttgt
    21001 gatttcttta tccggttctt tgccaatcat atcgatcacg tccatacgaa agcccccaat
    21061 acctttatcg atccagaaat tcatcatttg ataaatctct tggcgaacct gtttattttt
    21121 ccaattcaaa tcaggttgac gcttgctata caagtgcaga aaatattgcc ccgttttttc
    21181 atccaactgc caagcagaac cactgaatat tgagcgcaat ctattaggct cttttccatt
    21241 gaccggatca cgccaaatat aatagtcccg ataaggattt tctttatttt tacgggcctc
    21301 aatgaaccac ggatgttcat cagaagtatg attcaccacc agatccatga tgatacggat
    21361 atttcttttc tttgcttctt caatcagttc ttccatttct tccatcgtac cgaattctgc
    21421 catgattgct tgataattac tgatatcata cccattgtca tcattcggag actcgtatac
    21481 tggagaaagc caaattgcat caatacctaa atacgccaga taatccaacc tggaaataat
    21541 cccccgcaaa tctccaatac catcttgatt tgaatcttga aaacttcttg gatagatttg
    21601 ataaacaact gaatggtgcc accatttttg ttccatgatt ttcctcctat aaatggatgt
    21661 ttctattttt tgcttaatca tttattttag tctctctcac taaaaattgt aacgctttca
    21721 aatagctatt gcaccttgtt atcggtaaca gaataaaaca aaaaaacagc tgaggaaatt
    21781 ccctcaactg ttttttgaaa ggattgattt atgcagggaa gaatgctgat ttcacaactt
    21841 ctttataaac ggtgttcact cagctgtccc ttggtattag ctcaaaaaac ggcaaaatat
    21901 aagaaactat tttttcgttt ttttctctaa tactcaggcc agaacgctga tttcacaacc
    21961 tctttatttc acattcaata tccacatgaa tcccacaaat acaaagaaca gaccatacat
    22021 gattggagat acttcttttc ctcttttagc tgctaacatg gtaattggat agaagataaa
    22081 acctaatgca atcccatctg aaatactata agtcaaaggc atgccgatca agatcaagaa
    22141 tgaagggatc gcaatctcta agtttcccca ttcgatttgt cgcaaagatt gtgccatcaa
    22201 tactcctacc ataatcaatg ctggtgcagt tacttgtgaa gtaacaactg atagtaatgg
    22261 agaaaagaac aagcctaaca agaagaaaat ccctgtagta atagctgtga tacctgaacg
    22321 tcctccaacg gcgataccag ctgatgattc tacaaatgca ccaacaggag aagtaccaaa
    22381 taaggaaccc gcaagcatag ctgttgaatc agaagctaaa gctttcccta cacgaggcat
    22441 cttgttgtct ttcatgaaac ctgcttggtt tgctaatcca actaaagttc ctgctgtatc
    22501 aaagaaagtc acaagcaaga atgtcagcac tacgacccac atttgaagag aattgatgtc
    22561 ccctacatgt tttaatgcaa ctaaaaatgt tggtttcaaa cttggtgctg cggcaacaat
    22621 ttcagaaggc attggaatca aacctgtgac cagacctaaa atcgttgtag ctgccatccc
    22681 gatgaagatg ccacctggta cacgccgaac aagcaagata gcagttacga ctaatccaaa
    22741 aatcgtcaac caagtagaac caacgttgag tgatcctaat cctactaatg tagattcatt
    22801 cgaaacgatg atcccacctt cgctcagacc aaggaaagcg ataaatagac cgataccgcc
    22861 agaaatcgca tatttcaaat ctgctgggat cgcatcaatg atcatttccc gcagcttgaa
    22921 gattgtgatc aaaataaaaa tcaaagaagc aacgaatacg ccggctaatg ctgtttccca
    22981 tggaatcccc attcctaaac aaacagaata agcaaaaaaa gcattgatcc caagagcagg
    23041 tgccgttcca attggataac gggctaatac ccccattaaa acacagccga tcgcactggc
    23101 taatgcagtc gcagtaaaca ctgctccttc atccattcca gaagcaccta atacagttgg
    23161 atttacaaat aagatataag ccattgagat aaatgttgta aatcctgcca gtacctcacg
    23221 ttttaccgtg gtgttcaatt tttctatttc gaagtaggaa agtatttttt ccttcatctt
    23281 ccatccccct aaatccgaac attataaata aagtccgtta aaatttcgat ttattaataa
    23341 aacaacgtgt tttatcatat actaaaacaa aacataagta aacaaaaaat tcggatttta
    23401 agcaaaatcc gaacgttcca tctgattaaa tccctttatc tcaaagttca tgaacattca
    23461 ctttggatta caagaatttc tttattgtgt taataaagtt tcttcattct agtctcaaaa
    23521 atcaatagtg tgatttgatt ttaccatttc gtttctgttc ttctttgatg aattcttttg
    23581 tatcgacagg attcagatta tttagtccgc caaaacctgt atcgaccatt tttccccagc
    23641 cttttgtctt cttttttcct gatttgagat tctttgctcg aagcttctca ctatgttttg
    23701 ctcgccgata cttcaaataa ccttctaatc ttcccattag atccactgtt tttcctcctt
    23761 tttattcaat cagacgactc agcttttcct ataacaaaat aattgccttc agggtcagaa
    23821 aaattgaaag tcaagatgcc attgttatct gtgatttctc ctggttcttc catgttctcg
    23881 tagatagttt caaattcatc cgtaaaaaat actaaagaag gcggtggtcc taatacctct
    23941 ggtgaaaact tctgcacaaa tacttttggg aatacgccta attctaattc ttttgagatg
    24001 ctgagtacaa tactcttaaa ttcttctggc aattcgattg tttcagaaat ttctgcacca
    24061 attttttcta caaagaatcg gcaaatcatc tctacgtcat tgacataaag catcacacgt
    24121 gttttttgta tcatcatttg acccttcttt ctatttgatt ttctttcctg tctataaact
    24181 gattccatta tatcacgaaa gcgcaaacac cttgtgtttg atactattca agaaaaataa
    24241 agcaatagtt tctaggattg ctttcctcca caaacttttt ttacaaaaaa aaaacggcaa
    24301 tgaatgccgc tcttcttatt tcgattcttt tataattctg cttctgattc atctttggtt
    24361 agtgtgtgtc taacactagt tacgccaaat gtttgatcga ttttttcctt caacgcttcg
    24421 attgcctgtg cgccacgtgc cgcttgttca gcattgatgg attcaatgac cagcaccgct
    24481 gcttgtgttt cttccgtcag catcgttctt tgggcttcac gccagttgag acagcgacag
    24541 atcgctcctt gttcatcata gtagatgatt tcttccggaa gtgccggagc atcggaatct
    24601 gcacctaaag gcaggaatga ttcgccacct tgggcttttc ctaaatgcag atttcctgcg
    24661 atcttttcga tatcttctcc gccgcaaggt acgccaaaag acaaggaaat actattatag
    24721 atatctacaa gcggattgat tgggaaaaat tcccgttctt gatttgcacg tttcagtaaa
    24781 gcttcaatag aagaacgtgc acctttttta gtcttgaatg tggtaaatgc ctgacgccac
    24841 tgagcaatga cttcgttttg gctgaatgtt tcttctgtaa ggtaatttct tgcttctttt
    24901 gctgcctcat tcaataattg atgatacgtt tcttcacttt cttttgttac gtggttatcg
    24961 ataccattta cggataagat ttcgatggtt gcttcaggaa atacttccca gaaattggta
    25021 tccactatta cttttttcat ctgttcacac ctcttgatta gaataattgc ttgatgaata
    25081 gatagtctgt ttggggatca tctcccatat aaaaaacatg ttcgctgtat ttgataaatc
    25141 cttttttacg ataaaaagcg agcgcaacat cgttgtgttc ccaaacaccg agccaaatgg
    25201 ctgttttttg cttttctttt gctttttcaa tagctgtatc tagtaaaaat gtaccgatac
    25261 cttgatgttt aaattttttt cgtacataga ttcgctcgat ttctaaaaaa tgatcagcca
    25321 tttcctcaga ttgactcgtt cctgtattta gtttcaggta acccgcaggc tcttgatcga
    25381 tactcactag gaaaaaatcg gtttctttat cttttagctc ttctgtaagt ttttctagtg
    25441 cataagcttg tgtcaagtaa tcagctaaat gctccggtga attatctttt ccaaatgtat
    25501 ctgtaaatgt ttcgatacta atttgttgta gtaagtctac atctgaaggc gtcatttttc
    25561 gaatttcgta tttattcacg gcttcttcct ttcaatagat ccttttattg ccttttttga
    25621 caaaattcca atcttcgcta acattcttgc atactttatt taaaagatat tccaattgtt
    25681 ttgcttcttc gtcagaaaga ccatttaatg cgactgcatt ggagtagttg ttttctcgaa
    25741 tgatgaaagg atagatttct gctccttttt tagtcggata gagtttcttg atttttttat
    25801 tttctttatc ttccaaacgt tcgatcatcc cattggattc taatttcttg attgctcggg
    25861 cagtcgttgt acgatctact ttgatcattt ctgctagttt ttcttggatg attccaggat
    25921 tttcgcaaat acgtactaaa taaagatact gtcctcttgt taaatcgact tctttgaatt
    25981 cgatattgct aatagaatct agcgctcggg caatcattcc gatgtctcga aggatttcag
    26041 ccatttcatc ccacctttat tttgtttgta agactagttc acctcttttt gttgcaaatg
    26101 caactatttt caataaaaaa atcaagttct tttttaataa ctagtaaagt atattctttt
    26161 cttcttttac gaaaaagctc aatcacaata gatctggttt ttgttttcta aaataagtaa
    26221 ctgtttcttc gttgttcgct tttctgatag ctttggcaac tcttctatac taaaaaatcc
    26281 catgttggaa gtttcattat tctcaataaa cttggcctct tcaccgtgga tcgcacaagc
    26341 aaaaacaagt ttataatact gaaaaagctg tggaatatct ttttgtttat tcgtatcaaa
    26401 aactgctcgc aactgagtag tttctactgt taatcctgtt tcttcataga cttctttacg
    26461 cacattttct tcaggagaca atcctatttc agcaaatcca ccaggaagtg accattcttt
    26521 cgtccgttta tcttcgatta gtaggacctt gccatctttt ttgatatagg cacgaacatc
    26581 tacttttggt gttggatgtc cttctccaat cgttgtgagc gcttttattt cttcgaaagt
    26641 ttcttttcct atttcactga tcagttccaa tgaaatcgtc cggatctcct caaaacgctc
    26701 tttatcaaat acatcttttc cataatatag accagcttga gaaagtgcga gtagtctttt
    26761 gtattgagcc aacttcttgt tcattctctt cccttctttt ttagcaaatg tatgactatg
    26821 taatcaagta tagaagactg gtttaattac ggcaaataaa tataatgaaa tctgcaataa
    26881 ataaaagaat gttaaaaaac tcgagaacaa aagacattcg acttctctgg ggctgtgaat
    26941 aaatggcgtt caaaagcttg catctgcggt aataagcccc accaaacgaa aaatatgacg
    27001 agctattctt gtctggctgt tcttattaat tgaggcagga cagctttttc acaacctccc
    27061 cactaaacgg tgttctccta ccaactcctc ggtcacaagt cacaatattc atcaaacatg
    27121 agaagctgtt tttttgaaat tgctccttgt gactcggagc tgaacgacag gctcacgacc
    27181 tctcggtaaa cagcgttcaa aagccaattt ttcgatatta agccatttct ttgttactta
    27241 ttgtcattga tttctttttc taatttatcc aatagtggag tgtaatccac ttgagtatag
    27301 tctgttacat caaattcata tagtgttcct aactggaatt tgtcatattt tcggtcggcg
    27361 atgatctttt tgaatgcttc tttcgtaata tgattggtcc ctttcgttcg atcttccaat
    27421 acgtcaccat agtgataatg atccatgata tagctcaaat gacgatctga ttcacgatca
    27481 cgtttgtacc gacgttgcca taacacttca aagtctgctg ttaggcgaat cgtgatcacc
    27541 tgatatccgt atctatctgc taattcttgg agtcgtgctt tttgcttatc actaaaagga
    27601 tactcggaca caatcacttt ctttcctgct tccatataca aatcaagtgc tttgtaatag
    27661 aaaggataaa ctttcgtcct ttcttgttcc gcccgttctt ccaaattgtt aaacccgatg
    27721 gattctgcat agtattcctt gatttcatcg agcgtgatgg catacatatc tggaaatcgt
    27781 tcccgtagtt tattcataag atatgttttt cctgtaccag gactaccagc cagaagaatg
    27841 aaatactttt tcatctcatt tatttcctaa gttaattgga ctttccaagt tcataaacag
    27901 tccaatctta tgcttgacaa tttcttttac tgcttcattt gctggaatca cattatggcg
    27961 aagagacttg ttgatttctg tttcactaaa ggtttcttta gctactttcg tccagttgac
    28021 taaaagctcc gttccaacat tgaatttgcg gatcccgtta ttgatcaatt caggataatc
    28081 ttcttctttc acacctgttc ctccatggat aactaggggg acatcaacta ctgcatcgat
    28141 ttctttcagc aagggaatgt ttacttctgt ttttgatttg aattgtccat gattcgtacc
    28201 gatagcaata gctaatgcat ccacgcctgt agcttctaca aatttcactg cgtcttccgg
    28261 tcttgtatat accttgtcat tttcagcgac atggattcct tcttctgtac cgccgattgt
    28321 tccaagttct ccttctacgg aaacaccgtg cgcatgggca tattctacta ctgcttttgt
    28381 cttcaggata ttttctttga aaggcagatg ggacccatca tacatgacag aagtatatcc
    28441 gctgtcgatt gcttctttga tatcatcaaa gtctcgggcg tggtctaagt gcaagactgc
    28501 atcaaccatt tcttcttctg caatagctgt cattacattg actaacacat gcatgccgat
    28561 atatttagcg gtatcgacac ttgtttgaat aatgatcggc gaacccattt cttttgctgc
    28621 gcctagcata gcaggaagca tttctaggtt atgcgtatta aaagctccaa ctgtaaaatt
    28681 caactcttct gctgtttttg ttacttcttt caatgttgta tacatctttt attgccacct
    28741 tatcttttta tttgtttcct ttgatcgtta cttgttttgc aatttgtcgc atctcatcca
    28801 ttttgcccat gtagtaatcg atcccttgat catcaccagc acgagcagca tctgcacgct
    28861 tctggttata gaggctcatc agttttttat acccatctcc gatcgtctgt ttattttcca
    28921 aactcttttc taaatattgg atggcttgat cgttcacata caattcagaa aatttaagtc
    28981 caacttgttc ataaagtttc gctaatttat gcttatcttg ggtagtggaa atctcttgtt
    29041 tcaattgtgt aatctctgat tgaatctttt cggtcgcttc tggcgatagg gctttttctt
    29101 cttttgccag aacttttttc ggttcttttt cttttttctt aaaaaatcca aacatcattt
    29161 tccctacttt caattaaaat gctttcaaaa ctgggcttaa gatccaggca tataagagtg
    29221 ctgcggctac tgtatctaca tcggttgctg tagcattgtt gaatcccatc gaattaaaga
    29281 tcgttactag caaagcgggc aataatgtaa taaagaaacc atgagcaata ccgccaatga
    29341 tcgctcctcg gcgtccgcca actgcgttgc caaatatccc tgctgtccct ccggcaaaga
    29401 aattggttag cataccaggt aaaatcatcg ctaagccgaa catgggcaaa gtaaacatgg
    29461 caataatcgt accaatcgtt gtcgtaatga aacctaaaat gacagcattt gggctataag
    29521 gaaagaaaac tggacaatct aaggcaggga tcgcatcagg aactaatttc atagctatcc
    29581 ctcgaaatgc cggcacgatt tcacctaaca gtagacgtac accagctagc aatacataca
    29641 cacccactac gaattggatg gcttgtaaaa aggcaaacat gatatagttt tgatctcctg
    29701 ataattcaga agcgaatggt tcgcctgcaa ataaaacagt gatgatatat agcgggacca
    29761 tcacgaccat gacagacaaa taagtatctt gcagaaattc gaaatgtgtc ggcaacttga
    29821 tatcttcgat cgacttttta ttttcgcctt tctcaccaaa aagcttagca acaccggctt
    29881 caaataagta cccgatcgta caaaaatgac ctaaagcaat atcatttgat ccagtgattt
    29941 tacgaatgat cggttgcgct actgccggca tagcaatggc gaaacaagct ccgataaaac
    30001 caccgaccaa gatcaatacg atccctcgca gaccggcaaa atagccaaag actgtcgtca
    30061 tcgttgccat ccataaaatt gcttgaccag ttaaaaaaat gtacttccat tttgtgaaac
    30121 gagccaaaat aatattgaat acaaaaatgg ctaagaacgt cagtgcaata tctctaccta
    30181 aacccaaatc attcattgct tgaccattga tagattcaat agaagggatg atcccttgca
    30241 tatgaaatcc ttcagtaaag attttcccga aataagttaa acttccaacg atgatgctcg
    30301 aaccggcact caacacttga aatcctagga gtgtctttaa cgtgccagag atgacttgtc
    30361 ctgctgattt cttctgcagt agtagaccca acatagcaat caatgcaatg gtgatcgatg
    30421 cctgagtcaa aatattttcg atgataaaat tgataacgcc cat
//
LOCUS       DAFEPQ010000027        29986 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.027, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000027
VERSION     DAFEPQ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.027"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            23..400
                     /locus_tag="LP543_001357"
     CDS             23..400
                     /locus_tag="LP543_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321531.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            1006..1620
                     /locus_tag="LP543_001358"
     CDS             1006..1620
                     /locus_tag="LP543_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL2321532.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            2393..4132
                     /gene="yfcC"
                     /locus_tag="LP543_001359"
     CDS             2393..4132
                     /gene="yfcC"
                     /locus_tag="LP543_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBL2321533.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            4289..5473
                     /gene="iadA"
                     /locus_tag="LP543_001360"
     CDS             4289..5473
                     /gene="iadA"
                     /locus_tag="LP543_001360"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBL2321534.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            5584..5787
                     /locus_tag="LP543_001361"
     CDS             5584..5787
                     /locus_tag="LP543_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBL2321535.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(5888..6550)
                     /locus_tag="LP543_001362"
     CDS             complement(5888..6550)
                     /locus_tag="LP543_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2321536.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(6563..8065)
                     /locus_tag="LP543_001363"
     CDS             complement(6563..8065)
                     /locus_tag="LP543_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2321537.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8613..10292
                     /locus_tag="LP543_001364"
     CDS             8613..10292
                     /locus_tag="LP543_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBL2321538.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            10419..10898
                     /locus_tag="LP543_001365"
     CDS             10419..10898
                     /locus_tag="LP543_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBL2321539.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            11117..11797
                     /locus_tag="LP543_001366"
     CDS             11117..11797
                     /locus_tag="LP543_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBL2321540.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            11805..13136
                     /locus_tag="LP543_001367"
     CDS             11805..13136
                     /locus_tag="LP543_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL2321541.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            13151..14113
                     /locus_tag="LP543_001368"
     CDS             13151..14113
                     /locus_tag="LP543_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBL2321542.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            14224..14562
                     /locus_tag="LP543_001369"
     CDS             14224..14562
                     /locus_tag="LP543_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBL2321543.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            14641..15348
                     /locus_tag="LP543_001370"
     CDS             14641..15348
                     /locus_tag="LP543_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBL2321544.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15451..16932)
                     /locus_tag="LP543_001371"
     CDS             complement(15451..16932)
                     /locus_tag="LP543_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2321545.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            17241..17777
                     /locus_tag="LP543_001372"
     CDS             17241..17777
                     /locus_tag="LP543_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL2321546.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            17855..19225
                     /locus_tag="LP543_001373"
     CDS             17855..19225
                     /locus_tag="LP543_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBL2321547.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            19292..19627
                     /locus_tag="LP543_001374"
     CDS             19292..19627
                     /locus_tag="LP543_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321548.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(19708..20565)
                     /locus_tag="LP543_001375"
     CDS             complement(19708..20565)
                     /locus_tag="LP543_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2321549.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            20672..21187
                     /gene="tadA"
                     /locus_tag="LP543_001376"
     CDS             20672..21187
                     /gene="tadA"
                     /locus_tag="LP543_001376"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBL2321550.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            21210..21294
                     /gene="ffs"
                     /locus_tag="LP543_001377"
     ncRNA           21210..21294
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="LP543_001377"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            21504..22889
                     /locus_tag="LP543_001378"
     CDS             21504..22889
                     /locus_tag="LP543_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBL2321551.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTDIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            22926..23603
                     /locus_tag="LP543_001379"
     CDS             22926..23603
                     /locus_tag="LP543_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HBL2321552.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            23611..24375
                     /locus_tag="LP543_001380"
     CDS             23611..24375
                     /locus_tag="LP543_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBL2321553.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            24377..25036
                     /locus_tag="LP543_001381"
     CDS             24377..25036
                     /locus_tag="LP543_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBL2321554.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(25066..25575)
                     /locus_tag="LP543_001382"
     CDS             complement(25066..25575)
                     /locus_tag="LP543_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBL2321555.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(25687..26685)
                     /locus_tag="LP543_001383"
     CDS             complement(25687..26685)
                     /locus_tag="LP543_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL2321556.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            26886..27470
                     /locus_tag="LP543_001384"
     CDS             26886..27470
                     /locus_tag="LP543_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL2321557.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(27547..28740)
                     /locus_tag="LP543_001385"
     CDS             complement(27547..28740)
                     /locus_tag="LP543_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL2321558.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            28804..29184
                     /locus_tag="LP543_001386"
     CDS             28804..29184
                     /locus_tag="LP543_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBL2321559.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            29258..29620
                     /locus_tag="LP543_001387"
     CDS             29258..29620
                     /locus_tag="LP543_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321560.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         9760 a         5017 c         6211 g         8998 t
ORIGIN      
        1 taaaaaaagg tgtgtgttca ctatgggaaa attagtcgct gttgcacagt cgctgcttga
       61 tatactcaag tggctgatgg aaggaagcaa ttggatattt gtgttgccac tattgcttat
      121 tagtctattt atcatgtttc gcatggtcag aaaaaaagaa acgtttctga tttttatctg
      181 gtatgcagca tccgttgggg ttgctacctt gatggttgaa ttggcgttag gtagttggga
      241 agcttgcgta tttgctttat tttttgctgt actgtatcta atcatttata tcccatatat
      301 gaaaagaaag tctcggatag gcagaaaagg tagacataca actacttttt ctagtacaga
      361 ttctctcgaa caaagtagaa gaacgagaag aagacggtaa tatttagtag gtatacgcaa
      421 aaaaagataa aataatggat aaactattga gatgagagat ttatttaatt ggtggaaaaa
      481 agcttttttc acttatataa aagaatcttt tttaaaggaa tgcttgttgt atcgggataa
      541 gccattcctt tttttatgtt atttatatat atataaatca taccaatgtt tttttgtatt
      601 ttcttgcttg cacttggata agagattact tacaataaat tcagatagaa acagctgaat
      661 aaaaaaaggc tccctgaccc ttagttggcg ctaagaatca gggaatgacc agtcacacac
      721 atacaccctg accgatcatt ttgccatagt caatgcagtg catcgaccta atttattgta
      781 cctaatctag ggtgaaaatt ctacccctat gtagatattt tccatggtct gcacgttgtt
      841 ttatttgcta tgtcaaggta ttgggcgtat tcgttcaatg ccttttttgt gtttgaaaca
      901 gcaaagacca catgtgttac tgtctaacta ctgttttttt atgtacttcc ttagattagg
      961 cagctgtttc tattcatgtc aagtatagct aggcgggaga gataaatgag tttatgttat
     1021 tttgactatt atcaccaatt ggaagagaaa ttaaggatag aagagccgct gatgcttcaa
     1081 gctggcactt ggatcgcgaa acaaattaat caaggtggta gattgcagat atatgctagc
     1141 cgcacactaa gtggtgtagc atttgaattt tgggatcaaa ccccggatct tgtaccaagt
     1201 atattgattg aaaatccagc cggaggtgtc tatgaaacgc tagaaggtgc aggacaggca
     1261 attattgatc agctttctgt caaatcagaa gacatttttc ttcttctttc gaatgaagga
     1321 agaaacccgg caatcatcga actagctcag tggatcaaag aaaatggtca tccattaatc
     1381 attgttactg gatttgattt gtcacgaagt atcaaatcgc aacatagcag tggtttgcga
     1441 ttatttgaat ttgctgattt ggttttggac aaccacgcca atttacatga tgcagtcttg
     1501 actttgccaa atctagatcc agcgatctgc ggaacagcct ctcttgcaac gatctttttg
     1561 ctgcagcaag ttttgtattt tacagcaaaa cagctagtcc aggacacaag tggaaaatag
     1621 ttatccacat gttgacaaag ttccttaaaa aaataatgtt ccacataaaa atggagcgtt
     1681 tgaaaaaaag aaaacgatta ttaatgagga ttaacaacga ctactttttt tgttttagtt
     1741 attttaagat ggatcatctt tgattttaag cttgttttgt ttaaagtttg ttatttttat
     1801 aagttgtttc acagttatga gaaatgagtt ataagaatat gtagtgtcag aaaaataaaa
     1861 agtagtggaa aaagagacaa gaaagaatcc atatggattc tttcttgtct cttaatacac
     1921 gttgatgaaa gagtcttccc caaaactctt tacaaagtaa ttatagtaca tttgttatca
     1981 ttattgcaat gaagtttacc tgatataaca aaattgtcca acaggatacc tttttctaat
     2041 agaaataaaa tacacaaaaa ttgaaggata gataatggat atttataagt gtttattatt
     2101 ttttttgcaa tgaaattcaa tttttcaatg attgtaaaat gaaaaaaaca ggacgagatt
     2161 ttctctaatc taaaaataaa aaaagaattt taaggaaaaa cagagaaata aaatacttaa
     2221 tataaatgga aagagaaaaa aataatcata cataggagaa aaaaaacaaa atagaaaaaa
     2281 gtgattttct aataaattat tcatgtggaa atgtcatttt tactgtataa ttcatttgat
     2341 tatccatttt ttattcatca aaattactga aaaaagaaag gaagaaataa atttgaagaa
     2401 aacaaattct tcaggaaaac cgaaacgcga cttcactaaa ttatcgacac cccatacgta
     2461 tgtcattatt tttggtgtag tcatctttgc atggatactg acttttgtcg ttccagcagg
     2521 aaagttcagc acgcaagata ttgaatacaa agatgcaaat ggcgaaacaa gtactcgtac
     2581 tgttttacgt caagattcct tccgttatgc ttacgagctg gataagtcgt atgtgtttga
     2641 tcaattagaa gaactgcaag atcatccagc agaacgtgaa aaactggatg ttccggaaaa
     2701 aggattggaa aaagtcattg ctgacggaga aaaaaatctt acgcaggaaa aactagatga
     2761 aatctccttg acagatgatg tgctttacga tcagtacggc gaaaatatct atgatacttc
     2821 taaaaaattg cataaaacag ccaaaatatg gggaacagat gattttggcg gctttggttt
     2881 cttaaatttt gtttttgaag gactagtatc tggagataag tatggatctg ctgtagggat
     2941 cgctgcattg atccttgttg ttggaggtgc ttttggtatt attatgcgta ccggagcgat
     3001 cgacgcaggt atctatgcgt ttatcagtaa aactaaggga ctggagcgtt tagctttacc
     3061 gctattattc tttgcttttt cattcggtgg cgctacattc gggatggcag aagaggtaat
     3121 tccattctca atggtgatgg ttccatttgt cattgcgtta ggatatgact cgatcgtagc
     3181 agtaaccgtg acttatgtcg catctcaagt cggaaatgcg acctcttgga tgagcccgtt
     3241 tagcgtagcg gtggctcaag ggattgctgg aatccccgtg ttgtctggtg cgacattccg
     3301 attgattatg tgggtagttg ttactgcatt agccgctggt tatatgatga tatatgcaga
     3361 aaaaatccgt aaaaaaccag aaagttcgtt gacttacaaa tcagatgatt atttccgttc
     3421 tcatatcaaa aagacatctg atgaaaataa aaaattcatg cttgggcata aattgatctt
     3481 attagagatg ttagttgtcc ttgtctggat cgtttggggc gtgactcaaa aaggctatta
     3541 cattccagaa atcgcttctc aattctttgt gatgggatta gttgctggaa tcattgctgt
     3601 tcttttcaaa ttagatggaa tgaaagtcaa tgacatcgct tcttctttcc aatctggtgc
     3661 agctgattta gctggtacag caatcgtagt aggtatggcc aaagggattc ttcttgtctt
     3721 gggaggatct gatgcatctg ttccttcagc tttgaatacg attttgcatg ggatcgggac
     3781 ggcgttgacc ggtgttccag ctgtcattgg tgcttgggcg atgtacatct tccaaagttt
     3841 gttcaacttg gttgttacat caaattcagg acaagcagct ttgactatgc caatcatggc
     3901 tccactggca gatttagtag gtgtatccag acaagtagca gttcttgctt atcagcttgg
     3961 ttcaggtttt atggacgctt ttacacccgt atcagccagc ttgattggtg tgctaggtgt
     4021 tgcacggatc gaatgggcaa aatgggcaag attccaaatc aagatgcaag gattcctgtt
     4081 tgttttagga actattttca tcatgattgc cattgcaata ggtctgcagt agtactggag
     4141 acctgtaaat aaaaaagtaa tccaaacagt agataaggac tccaagaacc gtcattttac
     4201 ggtaaaatca aaggagtaaa gatcctatcg tttggattat tttttaagat cctatcatca
     4261 gttatactta aagaaagagg aaaaatgtat gaaaatcatt cgacaaattg aagtgtttgc
     4321 accagattac ttaggaaaaa tggatgtact gattgccggt gggaagattt tggctgtaga
     4381 agaacagcta gaaggcggct acgaaggagt ggaagtagaa gaactttcag gtgagggaaa
     4441 agtactaacc ccgggtttca ttgactgtca tttccatatt ttaggtggag gaggagaagg
     4501 cggttatcaa aatcgaacgc cagaagtcac attgagccaa ttgactacag ctggtgtaac
     4561 gacagttgtc ggttgtttgg gaacagacgg agaaggtaga gatatgactg cgttgatcag
     4621 caaagcgaaa ggtctggaag cagaaggaat ctccacttat gtatacgaag gttcgtaccg
     4681 attgccggta aaaacagtaa cagattctat catcaaagac tttttgacaa ttgataaaat
     4741 tatcggaatc ggagaaatcg cagtatccga tcaccgttct tctcagccaa gttttgaaga
     4801 gttcgctcgt gcagtagcag atgctcgtgt aggaggcatg ttatccggaa aagctgggat
     4861 catcaatgtc cacttaggcg gaggaaaaag aaagctagag ctattgacac gaatcgtaga
     4921 agaaacagaa attccgatca cacaattttt gcctactcat gcaaatcgca caccagaatt
     4981 atttgaagct tgcgtggcat ttgccaaacg tggtggcacg attgacttca ctgctagcga
     5041 agatcctgat ttttgggaga agacagatgg tgaagttcgc ttcagcaaag cactgaaacg
     5101 attgatcgag gaagatgttt ctttagataa tttcacgatg acctcagatg gacaaggaag
     5161 cttgccttat tttgatgaaa acaatcattt cctagggtta ggagtaggaa gtgccaaggc
     5221 attgctagta gggatcaaag aagctgtgca aaaagaaagt atccctttgg aaatcgcctt
     5281 acgagctata accagtaatc cagcacgtat attgaaattg gataaaaaag gaaaaatcga
     5341 aattggtgct gatgcagatc tgtgtatctt ggataaagaa acgcttgata ttgatacagt
     5401 tatcgctaaa ggagaaataa tggtccaaga aaaagaagtc aaagtatggg ggactttcga
     5461 aaaaagtttt taatcaacag ctctaactat cttttttaga aaacgagcgt tctgtttgtc
     5521 tgtatctgtt gttgatgtta ccctgaaggt agatcaataa acaaactaag aggaggaagc
     5581 acaatggcag atttaaaagg acgttctaaa gatatgaaag ataaagtagt cggtgaagga
     5641 aaagaagcat acggcaaagc aacagacgac aagagcaaag aaacagaagg gaaagcacaa
     5701 tcactaggtg ctgacatcaa agaaaaagct cgaaatttag gcgacgaagt caaagacaag
     5761 ttttctcgaa aagacgaaaa aaaataaaaa ccacttatta ctaattgaat caagcaaata
     5821 aaaaatccga accatatctg cgagacagat atggttcgga ttttttattt gatcattatc
     5881 ctgtttttta aacttcgatt ttttgaaatt ttttattttt tagttcaaag acttcatcac
     5941 agctttttgc tacttcaatc tcatgagtca cgatgataat acagcgattt tgttcatggg
     6001 cgatttcttg gaaaagtttg acgatatctt gtgaagttgt ttcatctaga tttccagtag
     6061 gctcatccgc tacgattagt tgatgttcac agcagatagc acgaacgata gcgactcgct
     6121 gctgctgacc tcccgaaagt ttggtcacct gtttttttgc taatatttca tcaattccta
     6181 cacgagccag tcgatctaaa gcaacttgtc gataattctc ttgcttggtc ttagtgattt
     6241 ccattgctgc tatgacgttg tctagagccg ataaataagg aagcaagttg taagcttgga
     6301 aaataatcga gacatcattt ttacgataac ttcttaaccc gattttgttc agtgaacgat
     6361 cttgatagta tacctttcct tcttttggtg catctagtcc agaaataagc gaaagaaaag
     6421 tcgtttttcc agaaccactt gctcctaaaa tagcgtacat ttttccagct tcgaaattca
     6481 gttctacatc tttataaagc gcatcttgcg gatcttgata ccaaaatccc aattgttctg
     6541 tccgtaacat caaattcctc ctttagttta ataggatttt tcttgggttt aatcttaata
     6601 tccctataga agctaaaaag acagaaaaca gactgattcc aaatgctact ccggctaata
     6661 gtcccaattg tgcaggtttg acattgattt ccaattcgct gacttgatca gaaacagcaa
     6721 atggattatt cgtggttcct tgttgaccat cgcgttgacc gccttgttct tgtctgttac
     6781 ctggcatttg tccaccgcca ggagcttgct ggttttgtgt cgtggtttct gtctgttggt
     6841 caagtagctg ctgtcccacg gcattcgcga cgacgtttcc gctaagcgaa gcaactccta
     6901 atgcaagaat catacaaata gccacttcgg taaacaattg gagaatgatt ttacttctgg
     6961 attctcctaa agatagcaag acgcctaact catgccgacg ctcccgaatg gtaatcataa
     7021 tgatcagagt aagtatgata ataccggcta cagccactag taaaacaaca tttttagaaa
     7081 aacttgccac gttgtttaaa ggttccaaca ttgactgata catggaatca tttgattgta
     7141 agctaaatgt atctgtgtcg atcagcttct cagcttcttt gagaaactca tccattttat
     7201 ctggatcaga tagagtgtaa atagcagaat ctatcgtgtt atcgtcagat gtcccattta
     7261 gttcatttgc aaaagtgtaa gaagtgtaaa gtgtattcga cggattcatg aagttgaatt
     7321 tcatacccat actgccgctt gtttcgctgg attcataaat tcctttgatc gtcatttcat
     7381 aagtggtatc ttcatcttca gtactcgtga ttgtgaaagt atctccgact gccaggtcat
     7441 ttgtttctgc tagagtagaa tcaatgacta cattgtttgt gcctttgtcg ctgtcatcta
     7501 ttccttcacc atccgtgatt tttgctgttc catcagaaaa attactattt tgtgcagttg
     7561 ctgacacacc actgatttga aagtctgctt gagtcatttg cggattcccg cccattcctc
     7621 cagcaggttc accgcctgca tcagtagttt ctgaactgtc ttcacttgaa tcagaactag
     7681 tgatggcagt tattccatct tttgccatag ctgaggcgga actttcaaag gaataactct
     7741 ttacattatc taattcagct attttttccg cgtccgttaa ggaaactggt gtaagagaaa
     7801 aactgcctgg atctggacgt tcttcagtgg tagaaccgct tgaagaagtt tgttcctgtt
     7861 ttttaaatga agcttctcga ttggcactta aagtcactgt tgctccaact tctttttgtg
     7921 cttgattcgc tgcttcttct gcagcgcttc gaatcgtgag gccagctagc acaaaaataa
     7981 gaatggctgt gaacacggca aacaataaca atgaacgtcc ccattttact tttgtacttt
     8041 tccatgcacg tttcataaaa ttcatatttt gcatccctcc tagtagatgc atttgataat
     8101 agacaaccta tcttaaagct agcttaaatc ttatgctgtt tgcacaccat caacatgagg
     8161 ttctgtatat ataaaaagtc actatagtta ttgcaaaaaa tgtggtaaaa tgaaaaaagt
     8221 atgtttcaaa ccgacgaaga agaaatgaaa gaggaatagc aaaagaaatc ctagatgcta
     8281 cagagggatt tttacgtgca tagttgttct aacaattgct gatactagcg atttagttta
     8341 tttgttgtat aataatgggg aacagctgaa tacttatata agtattctgg ctatagcgtt
     8401 tcgcaaaaga tccattttgt taatgaagta tatccaaaat aaaatgccca tccaactgtc
     8461 agaacgcgga tgtgctggta attttcctgc tgattctcca taagttgaga aatagtgatt
     8521 ttgcatatgc ttcattggaa tggctcatat atttagataa agaagaaaat cgacatcttt
     8581 aaacttggat gtcataagaa gaggtgaagt ttgtgagtac cattaaaatt atcccattag
     8641 gcggcgttcg tgaaaatgga aaaaacatgt atgtcgcaga agtgggcgag gatattttta
     8701 ttttagattg tgggctaaaa tatcctgaaa atgagttgtt ggggatcgac gtcgtaatcc
     8761 ctgattttac gtatttagaa gaaaacattg atcgtgtagc tggtgtcttt ttaacacatg
     8821 gacatgcaga tgcgatcggg gcacttcctt acttgttgtc aaaaatttct gtaccagtat
     8881 tcgggacaga gttgacgatc gagttagcaa aattgaatgt cagtcgtaat gaagcagcca
     8941 aaggatttaa ggatttccat gtggtagatg agcatacaga aatagacttt ggtgaaacag
     9001 tagtgagctt tttccgtacc actcatacga taccagattc agtggggatc aatctgaaaa
     9061 caaaagaagg tagtatcgtt tatacgggag attttaaatt tgaccaaacg gctatcccaa
     9121 tgtatcaaac agattatgca cgattggcgg aaatcggcaa agaaggagtc ttagccttac
     9181 taagtgaatc ggcaaatgca gaaaatcctc agcctgtagc ttctgaagcc caaatagcag
     9241 atgaagtatt tgatacgatc aaatattggg aagggcgtat catcgtcgct tgtgtagcaa
     9301 gtaatttgca acgagtgcaa caagtattgg atgcagcaga aaaagcacaa cgaaaagttg
     9361 tcttgactgg tcaagatttc gaacgaatta ttcgcacagc gatgcgttta gaaaaacttc
     9421 aattaccaag tgaagatctg cttgttacgt tgaaagacat gaaaaactat gatccagaag
     9481 aactgatcat tttagaaact ggccgaatgg gtgaacccat caagtctctg caaaaaatgg
     9541 caaacggtac acaccgtaat gtcaagattg aagaaggaga tttggtttat atcactacta
     9601 cgccaagcat cgcgatggaa accatcgtgg cgaaaacgga agatatcatt tatcgagctg
     9661 gtggaacagt gaagttgatc tctgagaata tgcgcgtttc tggccatgcc aatcctaatg
     9721 acttgcaatt aatgatcaat ttgatgaaac caacatattt tgtgccagtt caaggagagt
     9781 atcgtgaatt agctgctcat gcagatttgg ctcatgcagt agggatgcca tacaaaaata
     9841 tctatattac tggacgcgga gacgtgctgg aatataagaa caagcgtatg agtgttgcag
     9901 gaactacaag tgcagaaaat gtaatgattg atggattagg cataggtgat atcggaaaca
     9961 ttgttttacg tgatcgaaaa atcttgtcag aagacgggat tttcgtagcg gttgtcacta
    10021 tcagtcggcg agaaaagaaa atcatttcaa aacctcaaat tacttcacgg ggattcgttt
    10081 acgtaaaagc tagccgtgat ctgatgaaag aaagcagcaa tatgattgaa acaatcgttg
    10141 agaaacattt agaaagcaat gattttgagt ggagcaaatt aaagcaagac atccgagatc
    10201 aattaggccg ttacttattt gaacaaacaa aacgccgccc agtgatcttg ccagtcatta
    10261 tggaagctac tcaacgtcga aataaaaaat aaacaaaaag agtaagatga acctctctat
    10321 agagtgctcg tcttgctctt tttttgcgaa aaacaaataa tttttgaata cagcatccct
    10381 cgctttataa taaacgatag aataaaggga gtgaatcaat gatgagtcaa acaaaagtcg
    10441 aacgttattt agaagaacaa aagatcagct atcaaccact tgattttagt catgtggtag
    10501 aagaatcttt cacagaagaa ctacaaaaaa gagggattga tccagctttg atatgtaaaa
    10561 ctctcgtagt gaaaggaaac aaaactgggg tgatcatcgc attgattcct ttgaacgatc
    10621 acttagatta caaaaaaacg agacaagtga cgaaagatcg taaagttggt tttcctggaa
    10681 tggattttgt attaatgcat acaggatatc cccacggagc aaacacccct attggaataa
    10741 aactcgcaca tcctgattat tgttttttag cagacagttc tttaaaagaa aaagaagaag
    10801 taattgtgtc aagcggtgaa atcgggagaa gccttcggat aaaagtagcc gatttagaaa
    10861 agatcgtcca tcctatttac gctgatttaa ttagttaagg gacgagaaga gaagctaagc
    10921 agagcagaga cctcaaactt gagattatat ctgcttggta cttggtgcat cctgtattat
    10981 ctgttacaat tgctttgtac atactgtact atcaatccaa tgaaaatata tttagcagct
    11041 atattttttc attgatgtgc aagtgatgaa atcgttttta ataaaaaaga tacagagaaa
    11101 acgcgaggtg taaaatatga aaattacgta tcatggacat tcctgtttaa caattgaaat
    11161 ggaaaatggc caacgcgtga tgattgaccc gtttattaca ggaaatccat tgacagactt
    11221 aactgcagaa gaagtaaaga cagattggtt gctgattact catggtcata gtgaccacat
    11281 aggagatatg attcctattg caaaagaaaa tcaagccaca attatcagta ttgttgagat
    11341 tgctgattat gcgaacagca gaggagtaga ttcattcggt atgaatatcg gtggaaaaca
    11401 tgcgtttcca ttcggcgtgg taaaattcgt acatgcacag catagttcaa gctatgaagt
    11461 agacggtatc gttcagtata tgggcgagcc tagcggaatt attattcaag cagaaggaaa
    11521 aacgatttat catgcagggg atactgctta tttcagtgat ctaggattgc ttgcagaaga
    11581 attcgatatt gatgtcgctt tcttgccgat tggcgataac tatacgatgg ggccagaaga
    11641 tgcagcaaag gctgcgcaaa caattcgtgc caagaaagta gttccaatcc attacaatac
    11701 tttccctgtc attgaacaag atcccgaatc ttttgtagag ttactggaag atagtgaagg
    11761 aaaaataatg agcgtctcgg aaacgctgga agtgtaggca gaaaatggcg acaaaacatg
    11821 atttgatttt agaatatatt gaaagtttac ctgttggcaa tcgaatttct gtaagaagca
    11881 tcgcaaaaga attgaatgtc agtgaaggaa cagcgtaccg tgcaatcaaa gatgcagaaa
    11941 atgtcggact agtttcaaca attcaacgag tagggaccat ccgtatcgaa cgcaaattaa
    12001 aaaaacatat tgaacgtttg acatttggcg aggtcgtcag gatcattgaa ggggacgttt
    12061 taggcggggc ggcaggatta gacaaggtct tgaataaatt cgtcatcggc gcgatgacta
    12121 aacatgccat ggaacgctat attacgccag gatcactaat gatcgtcggc aaccgggcgg
    12181 aagtccaaaa attagcattg gaagatggcg ccgcagtatt gatcacagga ggattcaata
    12241 cgactgctga gattgcaaag ctagctgatg atcttgaaat gccgatctta cgcacctctt
    12301 atgatacatt tacagtcgca accatgatca accgagcgtt aagcgaccaa ttgatcaaaa
    12361 aagatatcat gcttgtcagt gatatctata tgccagctga aaagactcat tatttgcagc
    12421 agacagatac gatttctgat tatcagcgtt tgtcagaaga aacgcagcat tcgcgttttc
    12481 cagtcgtcaa ccgccatcat cgtttgatgg ggattgttac ggcaaaagat gttttaggga
    12541 aatcgccaaa ccagatcatc gatcgggtga tgacgaaaga accaatcagt gtaaagaaaa
    12601 caatgagtat tgcctctgtt agccatcaaa tgatctggga tgggctagaa gtcatgccgg
    12661 tcgtttccga tgacttgaca ttggaaggct tgattacccg gcaagatgta atgaaagcga
    12721 tgcagcttgt ccagcgacaa ccgcagattg cagatacgat ttcggatcag atatctggaa
    12781 atatccatac gattgatact gaccgtgaag gaaatcgttt ggaaaaccct aagttcaaat
    12841 tcgtagtagc accacaaatg gtcaatggcg ttggaaccat ttcttttggc gtacttagcg
    12901 aaatcatttc tgatgtcgca caaaaaacaa tggtgatgaa ccaaaaacga aatatcttga
    12961 ttgaacaagt gaacctacat tatttgcggc tgatccaatt ggaaagcgaa ttagacattc
    13021 gtccacgtgt attggaaatc ggcagacgtt ccgctaaact ggatatcgaa gtgtttattg
    13081 aaaacaccat cgtttccaaa gcaatcgttg tttgtcaagt aatggaacgt tcttagaaag
    13141 caggagtgaa atgacagtac agcaagaaat agtagataca ataaaaaaat acgataaaat
    13201 cattatccac aggcatttgc gccctgatcc tgatgcatta ggatcacaag taggattagc
    13261 cgaactatta cgggaaagtt tcccagaaaa agaaatacgt caagtaggtg aaacgattga
    13321 agggcttcgc tttttagcag aaatgcaaga agttgaagat gtcttctacc aaggagcgct
    13381 agtcatcgtg actgatacag ctaattcacc cagaatcagt gatcaaagat atcagttggc
    13441 agaaaaactg atcaaaatcg accatcatcc caatgatgac ccttatggag atcttgtgtg
    13501 ggtaaataca aaagcaagta gctgtagtga gatgattgca gaatttgctt tgatgtttcc
    13561 tgaatcttta aagatgaacg ttaaggctgc acggttactt tatgcaggaa tcgtcggaga
    13621 tacagggcgc tttttgtatc cctccacgac gtcccgtaca ttagagattg cttcgatatt
    13681 gagaaattat ccatttgatg caagtgcttt gaacagagag attgaacaaa tgccgatgaa
    13741 agtcgctaaa ctttccggtt atctttatca aaatatccag gtggatgaaa atggtgcagg
    13801 aagaataatc cttccgcaag aaattcttga taaatatgga atcgatgatt cagagacagc
    13861 ttcgatcgtt tcacttcctg gagtgattga cgaagtgctg gcttggggga tttttgttca
    13921 gcagccttca gggtattacc gcgtacgttt acgttctaag gggccgatca tcaatgaatt
    13981 ggctaaacgc catcatggag gaggccatcc attagcaagt ggtgcgaatg caaaagattt
    14041 agcagaagta gaggaaatct ataaagagat ccaagatata tgccaatcgt ataagagcag
    14101 aaaaacagaa tgatacttcg ttcgaaaacg tgccttttgt agtaaaaatc acaaatgtat
    14161 cttttcaaga tcttcttttt ccgttacaat agtgctcact aaagatttta aaggagaaag
    14221 aagatgacat taccaaattg cccagaatgt ggctcagagt acacatatga agatcgaggc
    14281 ttgttgattt gcccagaatg cgggaacgaa tggaacaaag aagaacaaga aactgtcata
    14341 gaagacggat tagtggtatt agatgcaaat ggaaaccgtc tgttagaggg cgatagcgtg
    14401 acagtgatta aagacttgaa agtaaaaggt gcgtcaggtg ccattaaaca aggaacaaaa
    14461 gtcaagaaca tccgtttagt agaaggcgat cataatatcg attgcaaggt agaaggattt
    14521 ggaccgatga aactgaaatc ggaatttgtg aaaaagaatt aaagaatatt caaggtcttt
    14581 tcacattctt ttcctactac taatgataaa ataaagtaaa cgaaaaggag tgaaacgaaa
    14641 atgtatggtt atggaatgta tggtttttta gatccgacat ttcttttggt cattcttgga
    14701 ttagttattt ccggaattgc gtcagcgtac gtcaatagta ctttccgaaa atacgatcaa
    14761 gtgagaagca aaaacaatgt gactggtaca caagcagcac aatatatatt acaaagtcaa
    14821 cagataaata atgtcggtgt tcaacaaatt gccggagatc tcacagataa ctacaattca
    14881 ggtaataaga tgttgagttt gtctcaagca actgcacagt ctacttcagt tgctgcgata
    14941 ggtgtagcag ctcatgaatg cggacatgcg gtacaagatg cttcgggtta tgtcccattg
    15001 aagattcgtg cagcacttgt tccagcagct aactttggtt cgatgatttc gttccccttg
    15061 attatggtag gtgtattgct tagctggaac tctacgttaa tcaatatcgg gattttcgct
    15121 ttttctttag cattactttt ccaattggtg acattaccag tcgaatttaa cgcgtctcgg
    15181 cgagcaatcc aaattttgag tgaaggcgga ctactgacgg aagaagaagt accgatggca
    15241 agacacgtat tatttgcagc agcactgact tatgttgcag cagcgctttc gacattcctg
    15301 cagctgttgc gcttgatctt attgttcggc ggcaaccgaa gagattaaaa taaagaagtt
    15361 gtgaaatcag cgctttgatc tgagctaata ccgagagtta gctgattgaa taccatttat
    15421 ttggaataag aggatgtgac aagaattttg tcacatcctc tttgttttgt tctcataacc
    15481 ttttttcata tacagttcat gaaccgcttt tattaacttg ccaaataaca ctgtattgtg
    15541 cgttgtatcc agatagaaag tctccgtttc atctgaaatt ttctcatgct gatctgtaat
    15601 gatcaaatca gcatgttctg aatatgcggt aattttaaca gcatctttgc gaaataactg
    15661 ctgcaaatgg ctttgaagat gataatttcc tgtaaactgt ttggttatct gcaaatggat
    15721 gagtaactgc tctttcgttt ctgtttgtag taagtgttgg atcaattgac taaggtattg
    15781 actgatttgg tttctttccc cattttttga aaaaattgta tcgatttttt ctcttaattt
    15841 cttttcctgt ataaaagaac tagaatgatt ggacgtggct tctggaaagc aaagtgtaga
    15901 aaaactactg ctggattcac ccaacagttc gaaaaaaaca actgccaaca caagaaaata
    15961 aaaataaatt ggtattttat tttctaaatg gttgagagga aactgttgag ttaagtaagt
    16021 catcaattga ccgacttgtc gacaaataag gtttttgctt gtggaaaata aaaaaccaat
    16081 ctcctgtttt tgtcgaaaag agatttgatt tggaaaaaaa caatagaaaa aataattaaa
    16141 gtaggctctc tctgtttctg tctcttcaca agggcgactc actgaaaaag aagtaaagta
    16201 ttctgaataa tcatgtgtat gctgcaataa gtccatcaat tcagtgattt cagaagaagt
    16261 ttttggaaaa tatgcctgtt gtttgtttcg taatatgaat aaagttaaca gcaagtagat
    16321 atttttttct tgttttgaaa ttttttctat ttgctcatct gtactgttca tttttagtgc
    16381 atttaattca agtgaggaaa atggccaatc gttacctcca aatgtatctg aaagaaaaaa
    16441 gtaaagatag agacggatag ataactcatt tccttcaaga gtacagcaat tttttacaga
    16501 taggatccgt aaatggaatt tttttaagga atgattgagc tgttttgtca gtttttcgag
    16561 ataggagggc gatatataaa gttgttcaag taaatatttt tttggtacag cagtttgtgg
    16621 ataattgatt agtaacatca aatagttaaa acgattggat tcttttaggt agcataattt
    16681 taacttagaa aaaacagcta actttttagt ttgatcggtt tctttggttt ggatctctcc
    16741 tttatgatta gaataaataa attcatcaat caataaaaat tgcttgagtg atacatttat
    16801 ttttcggatc agttctttga ttgtccgttt tggaatatgt aagtcattgg ataattcttg
    16861 taaagaacaa ttattttttt gaatgaccat ttgtagaaca agtaactctt tttttcttgc
    16921 ttcaattagc ataaatacct cctttttatt ctgtttttgt atatagttag acaaaaactg
    16981 tgagtggaaa gtcatttcag tctgtgttat tctcacagag tgtcgccttc ttttttatat
    17041 aacgttattt aatttttttg ttaccttgtt tattctattg tttatttcct agggtatttt
    17101 gtcaagatat tcttgttttt aaggcggaaa aattgatttt taatggatat aactaaatat
    17161 gttttgataa ttatcaataa tatttttaga attatttatg ttatttattt atgggtagat
    17221 gaaaagagac ggaggtaaga atgatgggga gagtaacaaa ttatagtaaa gaatatttgc
    17281 tgttcaaaag tatggtatac gtggaagaat atggaatgaa gtcaattact gcaagagagt
    17341 tagcagagtt ctgcggttgt tctacatacc cgatttatac acatttcaaa tcaatcagcg
    17401 ggttaaaaga gaagatatta gaagaaatca ctgtttattt tgaaaactac ttagctgaat
    17461 gcagttccga tgatgtttta agcattgtct acttattgaa agattttttt tctatccatg
    17521 aaagtattcg tgaaatcatt gcaaaatctg atttagacat ggattcgcta tttaagcggc
    17581 catttttctc atatgtcaaa ggtcacacga agatcaaaga agaaaagtcg cttgaattgg
    17641 tatggcttaa tgcattggga agtttgcgct cagatgattc ttctcaagat tttcttgaaa
    17701 tgatgaacgc tgtttgggat aaaaacgagg tgacggatga atacttggaa aatatcttaa
    17761 aggaacagtc accttaggca gcaacatgaa aaagtagatc agccaccttt atttttcgta
    17821 aaaaagattg taaaatgtct aacgaaacat tggaatgaag gaggaagcga caacgttacc
    17881 acagcaattt atcaaaaaat atcgtcttct tttaggcgaa gaagcatccg attttttttc
    17941 tgcgcttgaa caaggaagcg tgaaaaaagg atttcggtgg aatccactaa aaccggcagg
    18001 attggacatg gtccaaactt accatagcga agaattacaa cccgctccct acagcaatga
    18061 gggatttctt ggcactgtca atggaaagtc tttcctccat caagctgggt atgaatatag
    18121 ccaagaacca agtgcaatga tcgttggaac agctgctgca gcaaaacccg gtgaaaaagt
    18181 tttggatttg tgtgcggctc ccggtggtaa atcaacccaa ttagctgctc aaatgaaggg
    18241 gaaagggtta ttagtgacga atgagatctt tccgaaacgt gcgaagattt tatcagaaaa
    18301 tatagaacga tggggagttt cgaacgcaat agtgacaaat catgcacccg cagaacttgt
    18361 ccctcatttt tcgggctttt ttgatcggat cgtggttgat gctccttgtt caggagaagg
    18421 aatgttccga aaagatccaa atgcaataaa agagtggaca gaagaatctc cgctatactg
    18481 tcaaaaacgt cagcaagaga ttttgtcttc agcaataaaa atgctaaaaa ataaaggaca
    18541 actgatttat tcgacttgta cgtttgcacc agaagaaaat gaagagatca ttagctggct
    18601 tgtagaaaat tatccagtaa cgattgagga gattcctttg actcaatccg ttagttcagg
    18661 acgttctgaa tggggaagcg tagctggttt ggaaaaaaca atacgtatat ggcctcataa
    18721 agatcagggt gaaggacatt ttgttgcaaa gctgactttt catggacaaa atcagatgca
    18781 taaagaaaag aagacacgaa aaaaaagtaa agtgcaaatg acaaaagagc aggaaaaact
    18841 ctggacggag ttttcaaatg attttcatta tgaagctact ggaagacttt tagtgttcaa
    18901 cgaccatctt tgggaagttc ctgagttagc cccttctctc gatggtttaa aagtcgtacg
    18961 tacaggtttg catttaggag atttcaaaaa gaatcgattt gaacctagct atgcattagc
    19021 gcttgctaca aaaaagatag agaatatacc ttgcttgccg atcactcaaa aggaatggca
    19081 atcttacaca gcaggagaaa catttcaacg tgatgggaat caaggatggg ttttgctagt
    19141 attagataag atacctgtcg gatttgggaa acaagtaaaa ggaacagtaa agaacttttt
    19201 ccctaaagga ttgagatttc actgagtgga ttagttcgtt tatttttttg tctatgctat
    19261 gatggagata gataaaaagg agctgatact tatgaaaaca tcaacaaaag taacaatcgg
    19321 attaagcgta gcagccgctg ccagtgtagc gacagcagtc gttgtatcgg gaaaagtaat
    19381 ggaaaagatc catcatatga ctactcggac gaaagtaaaa aaattcgtct atgacaaatt
    19441 tgatggaaat gaaaaattac tagaaatcgt agatggtctt tcagatagtg acttggattc
    19501 tttgatggga atgttagcaa aaatcaaatc aggaaaaaag aaaatctctg tttatggtga
    19561 ttcaatcaaa gattcaaccg aagatgtaaa agaccggcta atgcattttg ttgaaaaaat
    19621 gatgtaagag aaactagctt ttgaacacta ttcattcgaa ataaggggtt gtgacaaaat
    19681 tattgtcaca acccctttaa tctatcttta attatgttca tgaagcaata aaagatagca
    19741 aaacagtaaa tcgttggctt tttttagatt gaaaccagac tgttcttgaa atttttcgat
    19801 tcgatatttt agagtattac gatgaagaaa cagtgttttt gcagcagagc tgacattgcc
    19861 taattcattc cataattctt gaatgatcac ttccatttca ttttgtttga tcagttctat
    19921 gtaacttttg atcaacttac tatcttttac agtatctttt gtatagtaat gtaaagccac
    19981 atcttgcata gaaaaagtac ggctgtgagt ataaagattg ttttgctctg ttaaaaaaat
    20041 ttttctttct tcagcaaaca aagggacgag gtcggtgcca ggtgaatgga aagaaccaac
    20101 gaatacagac gttgtcgtat caaaatcagc atctaaagat agaaaaatac cgttgatttc
    20161 agtagcatca agataatcac ggtttatttg ttcgactaag agcgcatctg tgtctgtata
    20221 aaaaaagaaa tcagcagaat agcgaaataa ttccaaaata gtatcttgcc attctttttt
    20281 taagaattct ccttttgatt cgacgtgaag ctggatcaaa cgaaagcttt tttcttgaac
    20341 acaagaagca tctttaaaaa gatagtgata ccatgggtgt tttttttgat cgttgattac
    20401 agggaataac gctttcaaaa ggctggtttc ttgttgagat aatgaatctt gtttgatcca
    20461 gataaaagca tcatcaattg caaaagaaag gatatccgct tctgatgctg gagagttttt
    20521 tttctgtgct tgtgggtaaa gagcaagtaa tttatccatt ttcatcttgt tcacctcttg
    20581 tatttatttt accatagaaa aaaaagttag ttacagcctt tttatcatat gctaaagtaa
    20641 aacaagaaaa aacaaaggca gggaagggta attggtaaat acaaatcttg atgcagtaga
    20701 aaaagaaaaa tggatgcgcc ttgcattggc agaagcaaag aaagcagaaa tgcttcatga
    20761 agttccgatt ggagcagtgg ttgtattgga tggaaaagtg attggaagag gatataacct
    20821 tagagaaacc acgcaagatg ctactacaca tgcagaaatg cttgcaatca aggaagcctg
    20881 tgaaaaagtg ggaagctggc gcttggaaga tgctgcactt tttgttacct tagagccttg
    20941 tcctatgtgc agcggcgcga tgattttgtc tcgtgtagca gaagtttatt tcggcgcgta
    21001 tgatccaaaa ggcggaacag ccggtacctt gatgaatctt ttggaggacg agcggtttaa
    21061 ccaccaagca tatgtagaag gtggcatttt agaagacgaa tgtgggatga ttttgactga
    21121 ctttttccga aagctccgcc agcgaaaaaa gaaattaaaa aaagactggt caacgaatga
    21181 aaaataaggt atactattct ttgccgaaag gctaggacaa tggtgggctt gtgaagcgtg
    21241 tcaggtctgg aaggaagcag cactaagcag gttccgccat gtgtctgatt ataatgaaac
    21301 gacaacagac tttcgggtct gtttttttta tttttatagt actgtaaaaa tgaatgttcc
    21361 tgatttttta gccagactga tttatcgaat attgtactta tacaatttgc ttcaaggcag
    21421 gaaaaactct gaaatagagc ttgtttcaat ggaagaaaaa gctgcctatg gtatgataaa
    21481 gataaagaga ggcgagaggg tacatggaaa aaatagtgat tttacatacg aatgatctgc
    21541 attctcattt agaaaattgg ccaaggatca gaaggttttt ggatcaacgt aaacgagaaa
    21601 acgaaaagaa agaaaaaacg acaacgattg cagtagatct aggtgatttt gtggatcgtt
    21661 ggcatccatt gtcagaagca acgaatggac aggaaaatat cttgctgatg aatgaagttg
    21721 gttatgatgc agcgacgata ggaaacaatg aaggcgtagg aaattccaaa gaagagttga
    21781 atcatttata cgatcatgct gagtttgata tcatcctgga taacttattc gataaaaata
    21841 cgctccagcc accggcatgg acgaaaccat ataaaatcat tacaacgaaa caccagacga
    21901 aaatcggact aattgcattt actgctccat ttccgcttac ttataatccg aatggctggg
    21961 acatacggaa tccttatgat attttgccag aattagtcga agatttacgt tcacaagtgg
    22021 atgttttagt tttgatgagt catctcggga ttcaggatga tttccaaatt gcaaaagaaa
    22081 taccagcaat tgatgttatt ttaggatcgc acacccatca tttattcaga gaaggtcacg
    22141 taatcaatga tgtccaaata gcagccgctg gaaagtacgg gcagtatgtc ggtgaagtcc
    22201 atttaaccat cgatgaagag aaaaaaatcc ttaagcattc agccaaagcc atccctaccg
    22261 aaacaatgac agcttttaca gaagatgaac aagaaataaa tagttatcta gaacgtgggc
    22321 atgaattatt aaaagaaaaa gaagtagcag atttgccatt ttctctgagt ctagatatct
    22381 tcaatgagca ttcgtttatc caagtagcct ttgaggcggt gaaagaacga ggaaagacgg
    22441 aagcagcaat attaaacagt ggtctgtttt taactgatat tccaaaaggg cgggtcaatc
    22501 aggatcagct tcacactgcg ttgccgcatc cgatgcactt gttaaatgtc acattgggtg
    22561 gaaatgatct gatccgctta gttttagaaa tagaaaaaaa tcgtaacttt ttacgtaact
    22621 atcctatgaa gggaatggga tttcgcggta agatttttgg gcagatggtc tataatggaa
    22681 tcacatatga ttctattaac catcaagtct tttggaagaa caagccaatc gatctgaata
    22741 aaaattatac ttttactacc gtagatcatt tgatgtttgt tccatttttt ccaacgattg
    22801 agattgctgg aaaaaatgaa tttttgttcc ctgaatttat ccgaagtgtg gtaggagatt
    22861 acttgaaagc tcactaccca atcaaataaa aacaaggtat aataaaaaag ataggtggtg
    22921 aacgcatgtc tgcaaaacaa aatgaacagc agaacgttga gcataaaaca gaaattgcta
    22981 agaaaaaatc atcagctgaa caggaaacaa cagtcaccga agaactgaca gctgctttgg
    23041 aagaaatcgt tgaggagcct aaagcagaaa aagtcaaagg cgagctagtg acactgttag
    23101 atgacagtaa ttttttgata ggtgaaagac attatcggct tgtgtccgat tatcgtgaag
    23161 gatttaatgc agaaaagctt ggagaacgtt acagtgatgt actagcccga tatgattata
    23221 tcgtaggaga ttggggttat gaacaactac gcctaaaagg atttttccaa gcggataatc
    23281 gacgggcgca tccagatcag cgaatcgatt ccttggaaga ctatctttac gaatattgca
    23341 attttggttg tgcgtatttt gtcatcgaga gaatcggcgg taaaaaagaa aagacaactc
    23401 agcgccgaaa aaagaaaaaa aaccataatc gtgcgtatat cgatgagaaa aaaggaccag
    23461 tcgcatctaa tcgcaaaaaa ccggttatta aaaatcgaaa aacagagtca gcaaaaacct
    23521 ctgaaaaagt aaaaaaacaa gaacaaccta aacaagtaga aaaaacaaaa acagcattta
    23581 cgatcagaaa aagggaagag tagtggatga atggactata aaggatattt aattgattta
    23641 gacggaacga tttatcgtgg gacacaaccc ataccggccg gtaaacgatt tgtggaagaa
    23701 ttgcaaaaaa gaaaattacc tttcttgttt gttaccaata acacgactaa gtcaccagaa
    23761 acagtagcta atcgtctagc agatgagttt gacattcatg tagctccaga gacagtctac
    23821 acagctactt tggcaacaat cgattttatg aaagcagatg gaaaagggaa aaaagtctat
    23881 gttatcggag aagcaggatt gattgacttg attttagctg ccggatttac atgggaagaa
    23941 gaggctcctg actatgtagt ggtaggctta gataattatc tgacctatga aaaagtagtt
    24001 aaagcaacac ttgctattca aaaaggtgct actttcattg gaacgaaccc agataagaat
    24061 atacctactg aacgcggcct tcttcctgga gcagggtcag tcatctcttt tgtagagaca
    24121 gcaacccaaa cgccgccgat ctacatcgga aaaccagaag ccatcattat ggataaagcc
    24181 gttgaagtat tagggctgca aaaagaagaa gtaatcatgg ttggagataa ctatgaaaca
    24241 gatatccaag ctgggatacg caataatata gatactttgc tcgttttatc aggttttaca
    24301 aaagaagaag atgtacctgg gttgcctatc ccagctacct atgtgaaaca atcactggat
    24361 gaatggagct tctaatatga aaaattcgag atggcagtgg atggaatatg ctggattgtt
    24421 cagcctattt ctgacactga tcagtctggc agttggtgtg acgatcaact ttcgaccact
    24481 atacgtattt gatatcggac atttgcaaat tttggattat acttctttag atcaagagac
    24541 gttactgaaa aattttgatc acctaatgaa ttatctcaat aatcccttca aaaccatcct
    24601 cagcttgcca gatttcccag tttctgctag cggagcccat cacttttatg aagtgaaaat
    24661 tttgttttta gtggattatg ctgtgttttt catcactttg atcccgagta ttttgtttat
    24721 caaatatttg caaaagaatg atcggctttg gcggttgatt cgtccgtttc aaatcggcat
    24781 gttactgcca gttgttttcg gttttttcat gatgatagga tttgatcgct tttttatcct
    24841 ttttcatgaa acgtttttca acaacgatga ctggttattc gatcccgtta ctgatccaat
    24901 catcaatgta cttccagaag aatttttcat gcatagcttt atcttatttt ttgtattatt
    24961 agaattattt tttgctgttt ttcttttctt aggaaaaaat agcttgaaac aaacaaaaaa
    25021 gaaagaactc gtctaaatag acgttttctt tctttttttg ttgaattatt gagtaaggcg
    25081 acttcttttt tctggttctt tgtgtgctaa tcgactggca gcagcagcgg cgatcgctcc
    25141 gacgatatca tctaagaagg tatggacatg aacgccatcg tggctgttta gttcttttaa
    25201 aataccaggt ttcaccttgt cgatataacc ataattcgta aagccgatcg taccgtaaac
    25261 attcacgatt gacaaggcta ggatctcatc gataccgtaa aggccttcgt catcttcgat
    25321 aatatgttgc aacggactca tcaatttttt cttttctgct aatatatcta attggattcc
    25381 agtgataatg gcgttatgta cttcacgctt tgttagcacg ctgttgacac tttcagtaca
    25441 atccgccaaa gtcaaatcag aaatatagtc tttttgtaaa aacatgacaa gttgtgcgat
    25501 atcttctaca gttacgccac gttccttcaa tagttctctt gccttttctt ctaaagtctc
    25561 agttttgacg accatattat tgctccccct ttattttttc tttctctatt tcatgttaac
    25621 ggaacatgaa aaaaatgcaa gaaactgctt tcgggtttaa cttgaaatgc aaagagatat
    25681 aaaaaatcaa ctaggacgaa gatttttatc gtataagctt gtgctgtgac caggctgagt
    25741 tcgttccttt ggattaatat agtgtaaagc gttgttcact gcagtaggtg cttcaccgag
    25801 cccagtagca atcagtttca cttttccttc ataggtacag atgtcaccag ctgcataaac
    25861 gcctggaata gaagtagaca tatctgattt tacagtaatg gcatttctgg tactatcaag
    25921 tccccaagaa gaaagatgtt ctaaattaga agagaatcca taattgacga tcagagaatc
    25981 aatcatcaaa tcaatcgtct cgtcactttt cacttttttt aaccggatat ctgtcagttc
    26041 cccattattg cctgataatc catcgatgag atagggagta agcaagttga cagatgaaga
    26101 tttcaagcgt gatacactat gttcgtgcgc acgaaattcc gggcgacgat gaatcaagta
    26161 aacttcgctt gcaataggct ctaacatcaa cgtccaatcg atagcagaat caccacctcc
    26221 agcaatagcg actttttttc cagcatactt catcaaatca ttcacgaaat aatccaatcc
    26281 gtggttttca aatgattcag cattatctaa attcaatttt cttggctgga atgaaccatt
    26341 tcctaaagct aaaatgactg ctttggaata atggatgcct ttatttgtag taatctcgat
    26401 aacttcgtct tcacgagtaa gcgagagaac ttcttctttt aaatggaatg tatggttaaa
    26461 agtagttaat tgtttttcta attgatcgat taattcactt gcttttattg ctggataccc
    26521 aggaatatcg taaatatatt tttcgggata caaagttgct aattgaccac caagttgagg
    26581 tagactgtca atgatttttg ttttagcttg gcgcattcct gcgtaaaaag cggcaaacat
    26641 ccctacagga cctgcaccga tgattgtaat gtcatagatt tccataaaaa ttcctccctt
    26701 ttcacactat agcagaattt aattgagaat gcttctcaaa aatgtttgaa ttcgaaatcg
    26761 aaacggtaag ttatagaatg tatctagtga tgaaaaataa aagaaaacaa atctagtatt
    26821 tttacgagag gttaggtatt atgttattat tattatgaag tatgcaaata ttaggaggaa
    26881 acaatatggc attcccacaa ctagatttaa aaagtgaaaa aggaccaaaa gctgttatca
    26941 aaacaaaccg tggtgacatt actgttcaat tattccctga actagcaccg aagacggttc
    27001 aaaatttcat tgaactttcc aaaaaaggtt attatgatgg cgtgattttt caccgcgtta
    27061 ttcctgattt catgatccaa ggcggcgatc caactggaac aggaatgggt ggcgaaagta
    27121 tttatggcga aagttttgaa gatgaattca gtcgtgaact gttcaacctt cgcggagctt
    27181 tatctatggc aaattcaggt ccaaacacaa acggaagcca attctttatt gtaaataaca
    27241 caaatgtgcc agctaatatg cttggacaac tagaaggtgc tggattccca tcagaaatca
    27301 ttgaagcgta taaaggtggc ggaactcctt ggctagattt ccgacataca gtctttggac
    27361 atgtactaga agggatggac acagtagatg aaatcgcaaa tgtacaacgt ggtccacaag
    27421 accgacctgt acatgatgta gtgattgaaa caattgaaat ttcagaatag tttggtatgg
    27481 tacggagtaa ttatgaaaaa ggacgaaacc tagtaaggtc tcgtcctttt attttgaata
    27541 aatgatttaa tcgattgtct ctaaagcata cttgattcga ttaagtcctt ccagtaaagt
    27601 ttcttctgga caagcaatat tcaagcgcat atgcccatga ccggaaggac caaacgtgat
    27661 gccaggattt aatacgactt ttcctttccg aacaagagtg ttctccaatt cttggtcgtc
    27721 tgaaatatag gcggtaaaat ctaaccacat taagtaagtt ccttcagggt tcattattct
    27781 cactttcgga agatactgtt caaaaaactg attaactgtt tgaacatttt tttctagata
    27841 aggaatcagt tggcttaacc attctccacc cgtctcataa gccgcttggg ttccaatcag
    27901 tccaaaagtg ttgatttcgt gctgttggtt catcactaaa tgtttttcaa aatcagcttt
    27961 catctctgga tttttgataa ataccattga atttttgatt gcagctagat tgaaagtctt
    28021 ggttgcggaa gtaaaagcaa ctagtagatc atctaaatta ggatcaatcg ttagcatgga
    28081 agtaaatgta tgatcgaata aagttaaatc ttgatggatc tcgtcactaa acagaatcac
    28141 ttgatgtttt aaacacaatt cgcttaactg tttcaattct tctttgctcc aaacacgccc
    28201 tccaggattg tgaggattgc ataaaatcat tgcttttaca ttgttttctt cgattttttt
    28261 ctccatgtca gacaagtcca tgatgaaatg attatttttt tctagcaaag agcttcgaac
    28321 gatgtgacgt tgatttgttt ctataataga ggaaaatgga ggataaactg ggtcatgaat
    28381 caaaatagaa tcgcctggtt ttgtaaaagt ttgtactgct gcagctaaac tggttaacac
    28441 accacttgta aaaacgatat tttctttcgt caactccaca gcatggtgtt ggcgttccca
    28501 atgaatgata gcctgatata gtttatcaga cagagtggca taaccaaaga ttccatgttt
    28561 gatgtattca ctaaacgctt tagctacccc accgggagac aagaagtcca tatctgctac
    28621 ccacaaagga agtaagtcat ccatttgata tgtctcagca attgcgtccc attttacact
    28681 ttctgtatct tttcttgaaa tctgtttatc aaattcgatc atcttaagac ctccagtcat
    28741 tttcgtcgct tctttttttg agctatttac agtataatga taaatgcgaa gagagggaag
    28801 tagatgactt ataaaattgg acaaataatt gaaggaaaag tgacagggat acagccttat
    28861 ggcgcattcg tttctcttga taacgagacg caaggactta ttcatgtttc agaagtacaa
    28921 tccggttata cgaaaaatat ccattcattg ttaaaagtcg gacagccagt aacagttcag
    28981 atcatcgaca ttgatgaata ttccaaaaaa atcagtttat ctttaagaac gttagaaaaa
    29041 acaaatccag ctgttccata tcgcagaaaa aggtatttca cgaataaaaa tcgtaagatc
    29101 ggatttacca ctattgcgga gcagttacct atttggatag aagaagcatt atctgaacta
    29161 gaacggaaag aaaataagaa ataaaaagtg tattttttct gaaaatgtgc tagaattttt
    29221 atgtaagcca tagaacaagt tgatcgaggt ggaaattttg gcaagcaaaa gagttgctgg
    29281 tacaattatg ttgcatttgg aggatggtac taaaaaattt ttggtccatt caattgatga
    29341 caagttggaa tttgcgttag cggaactttc tgaaggaaag actggtttag cgaatatact
    29401 taatttctta aaagaaatag ttcatatcga tgttagcaag attagtttga tggaattgac
    29461 aaatacacat attaaagagg aaaatgttcc gttgtttgtc tttgaaactg aacaaaagaa
    29521 tcaaagagaa gaattaggcg aaggatatat ttgggaagaa ccaggtcaaa tgcgctctgt
    29581 tcttggaact tacgaagtag agggagtgcc tttcttctaa aatacagatt aagcgaacaa
    29641 agaagtttga atttttttca aacttctttt tttattgttt ggtccaaaaa atacaatgaa
    29701 atgttctgct aaagatagtt ttttagtgca ttttattttc aaaatatgtt gaaaacggtt
    29761 gtataacgaa taataataat tgatcgaaaa atcatttcac aaagtaatgc ttaatttata
    29821 gttgaccaag gaagaaatac atgataaatt taacagcgtt gattctgaag tggatgttat
    29881 atcacatttc taaaaaattt aaaaaatagt tattgacatg ttactaacga catgataaga
    29941 taactgagtc gttaaaaaag aaccacttgc gaagcttgat tctatc
//
LOCUS       DAFEPQ010000028        29118 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.028, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000028
VERSION     DAFEPQ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.028"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            243..2522
                     /locus_tag="LP543_001388"
     CDS             243..2522
                     /locus_tag="LP543_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL2321561.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(2615..3262)
                     /locus_tag="LP543_001389"
     CDS             complement(2615..3262)
                     /locus_tag="LP543_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321562.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(3441..4160)
                     /locus_tag="LP543_001390"
     CDS             complement(3441..4160)
                     /locus_tag="LP543_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2321563.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(4291..5442)
                     /locus_tag="LP543_001391"
     CDS             complement(4291..5442)
                     /locus_tag="LP543_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBL2321564.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(5659..6399)
                     /locus_tag="LP543_001392"
     CDS             complement(5659..6399)
                     /locus_tag="LP543_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL2321565.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(6403..6726)
                     /gene="rsfS"
                     /locus_tag="LP543_001393"
     CDS             complement(6403..6726)
                     /gene="rsfS"
                     /locus_tag="LP543_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBL2321566.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(6783..7376)
                     /gene="yqeK"
                     /locus_tag="LP543_001394"
     CDS             complement(6783..7376)
                     /gene="yqeK"
                     /locus_tag="LP543_001394"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBL2321567.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(7360..8010)
                     /locus_tag="LP543_001395"
     CDS             complement(7360..8010)
                     /locus_tag="LP543_001395"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBL2321568.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(8020..8331)
                     /gene="yhbY"
                     /locus_tag="LP543_001396"
     CDS             complement(8020..8331)
                     /gene="yhbY"
                     /locus_tag="LP543_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBL2321569.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(8347..9456)
                     /gene="yqeH"
                     /locus_tag="LP543_001397"
     CDS             complement(8347..9456)
                     /gene="yqeH"
                     /locus_tag="LP543_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBL2321570.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(9453..9980)
                     /locus_tag="LP543_001398"
     CDS             complement(9453..9980)
                     /locus_tag="LP543_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBL2321571.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(10160..10945)
                     /locus_tag="LP543_001399"
     CDS             complement(10160..10945)
                     /locus_tag="LP543_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBL2321572.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(10938..11807)
                     /locus_tag="LP543_001400"
     CDS             complement(10938..11807)
                     /locus_tag="LP543_001400"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBL2321573.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(11808..13187)
                     /locus_tag="LP543_001401"
     CDS             complement(11808..13187)
                     /locus_tag="LP543_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBL2321574.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(13195..13614)
                     /gene="fabZ"
                     /locus_tag="LP543_001402"
     CDS             complement(13195..13614)
                     /gene="fabZ"
                     /locus_tag="LP543_001402"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBL2321575.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(13639..14115)
                     /gene="accB"
                     /locus_tag="LP543_001403"
     CDS             complement(13639..14115)
                     /gene="accB"
                     /locus_tag="LP543_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBL2321576.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(14118..15353)
                     /gene="fabF"
                     /locus_tag="LP543_001404"
     CDS             complement(14118..15353)
                     /gene="fabF"
                     /locus_tag="LP543_001404"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBL2321577.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(15372..16109)
                     /gene="fabG"
                     /locus_tag="LP543_001405"
     CDS             complement(15372..16109)
                     /gene="fabG"
                     /locus_tag="LP543_001405"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBL2321578.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(16121..17038)
                     /gene="fabD"
                     /locus_tag="LP543_001406"
     CDS             complement(16121..17038)
                     /gene="fabD"
                     /locus_tag="LP543_001406"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBL2321579.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(17145..17369)
                     /locus_tag="LP543_001407"
     CDS             complement(17145..17369)
                     /locus_tag="LP543_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBL2321580.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(17422..18384)
                     /locus_tag="LP543_001408"
     CDS             complement(17422..18384)
                     /locus_tag="LP543_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBL2321581.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(18394..18834)
                     /locus_tag="LP543_001409"
     CDS             complement(18394..18834)
                     /locus_tag="LP543_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL2321582.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            19140..20105
                     /locus_tag="LP543_001410"
     CDS             19140..20105
                     /locus_tag="LP543_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBL2321583.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(20162..20392)
                     /locus_tag="LP543_001411"
     CDS             complement(20162..20392)
                     /locus_tag="LP543_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBL2321584.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(20478..21365)
                     /locus_tag="LP543_001412"
     CDS             complement(20478..21365)
                     /locus_tag="LP543_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBL2321585.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(21454..22677)
                     /locus_tag="LP543_001413"
     CDS             complement(21454..22677)
                     /locus_tag="LP543_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL2321586.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(22694..23740)
                     /locus_tag="LP543_001414"
     CDS             complement(22694..23740)
                     /locus_tag="LP543_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL2321587.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(23904..25631)
                     /gene="ptsP"
                     /locus_tag="LP543_001415"
     CDS             complement(23904..25631)
                     /gene="ptsP"
                     /locus_tag="LP543_001415"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBL2321588.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(25631..25897)
                     /locus_tag="LP543_001416"
     CDS             complement(25631..25897)
                     /locus_tag="LP543_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBL2321589.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            26376..28610
                     /locus_tag="LP543_001417"
     CDS             26376..28610
                     /locus_tag="LP543_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL2321590.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            28758..29078
                     /locus_tag="LP543_001418"
     CDS             28758..29078
                     /locus_tag="LP543_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBL2321591.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         8293 a         6282 c         5059 g         9484 t
ORIGIN      
        1 tagtaataaa tttccagctg cctctttcat cttaagcata gtccttgact agcacttttt
       61 aaaacaatag gccttaattg aaaacaacta tcactatcag cgtttccaaa attcatcagt
      121 tctacaaaca aaaatattca gcaaggatta ctttttcgat tcatcagtta aaaagtacct
      181 tttttcgaaa aactatgcta tacttttaat tcgttattta cggcggaagg agcaaacatg
      241 cgatgaatga gaaacaacta gaacaaacgc acgtggatga aacgatcgaa ctaatccaga
      301 tcgaacaaaa tatgctggat acaaaacaac aagaattgac tcaggaaatg aatcattcga
      361 caaaagacac agctaatcac aagattcgag ctggttccaa tgaagctttt tatgaatctg
      421 ctgttgaata tcgccaacat gagcaggaat tgcttttgaa ataccatact cttgaagcac
      481 agcaaaaacg cctgcaaacg ttgtctgtca tgaaagatag tccttatttt gcccgtatcg
      541 actttaaaga agaccgtgaa acagaaacat tatatctagg tatcgcttct ttaagagatc
      601 tggcagaaga aacaatcgtt attgactggc gcgcaccaat tgccaatctt tattatgaag
      661 gtgagattgg tcccgcattt tatgaaactg aaacagatag aatagatgtc gatctgcagt
      721 tgaaacgaca atttaaaatc caagaaggac gtattttatc tatggtagat acgtcggaag
      781 taatcaatga tgactttttg ctagagatat tggatgaatc atcaagcaac catatgaaaa
      841 acatcgtctc tacgatacaa aaagcacaaa atgcaatcat tcgggacaca acaagtaaag
      901 taatgctgat tgaaggaatc gccggaagtg gaaagacatc cgctttgctt caacgtattg
      961 cttttctgtt gtatcataat cgcaagtggc tcgaggctga aaacgttctt ctattttcac
     1021 ccaaccattt gttttctgat tacatctcga ctgtattgcc ttcattaggt gaaagcggcg
     1081 tgcctacaca gacattcaaa aattatttgg tgcagctgct gccagagttt tctttactag
     1141 atgaacagca acaagaaagt ggttttttgc taggggaaaa agatccgata caagtgatga
     1201 aaagcggatt gaccttagtc gatcagattg atcgctatat ccaaggcatc acaaactacg
     1261 gtcccttgtt ccgtgatatg aaaataaatg gccgtacgat cctatcaaag gaatcgattc
     1321 gccaatggta taaagaaaca aatgaacaat tacctttaca tcatagactt tctctattac
     1381 aaacgaagct cctgaaaaaa ttaggcggtc tgcaaaaaga cgaaacacgt cagcaatggg
     1441 tcaaagatct ggcggaagaa cagctccaag aactatacgc aacagatcca aatcttgaat
     1501 atacagaaca aaaggagcgt tcattacgta aaaaactggc caaccaaatc gtacgtaaaa
     1561 gattccgtcg aatccatcga gggatcatga attatcaatt tgtcaatata acgaaacaat
     1621 atctccactt tctacaaact gtgccacttt ctcttctaac acgacataaa atcagcgaga
     1681 ccaggtggca ggaacatttg acaactgttc gtacacactt gcgtcaaaga gaattgcgtc
     1741 aagaggatgc tgtactattc tttttactga tgcgccgaac atatccagtg acggttacac
     1801 acaaagctcg ctatatcttt attgatgaaa tgcaggattt cgcaccatca caagtggcat
     1861 tgctgcaagc cttgttccca aaagcaaatt tgactttctg tggagatcta aatcaaaatg
     1921 tctttggaaa tgaaacgatc gttggatcat tggaagacct tttcgtggac cgtgaagtca
     1981 ctcgtttcca attaacgaca agctatcgct ccacaaaaga aatcactgat tttgctaatc
     2041 attttattgt gaaagaacat caagtagaaa caacagcaag aaaaggagac cttcctttgc
     2101 tcatccaagg aacaacagat gatatcaaat tgaattggtt agcagatacc attcgaacga
     2161 ctcagggaaa agcccggtat tggcgtacag caatcatagc taaaacacaa gaagaatgcg
     2221 aagctcttta tgctcaactg ccagacgagt tgaaacaaca agtccagctg attttaaatg
     2281 agagtgactt tatgaagcgt tccattattt tacttccagc ctttttagca aaaggtctgg
     2341 agtttgaccg agttttcttg tgggatgtag atgcagaaca tttttcttct cagcaagaca
     2401 aattgatcct ttacaccatg tgtacacgtg cgatgcatga attgaccctt tttacctcca
     2461 ataatgaaag tcctttgtta aacaatatgg atatttcatc ttatgagaaa aaagtactgt
     2521 aagtaaaaag acaactgcta tcattaatgt tagcagttgt ctttttattt acaataataa
     2581 ttgcagaaaa agtaattcaa tcatttaagg tcatttattc tatatataaa tccaaatctg
     2641 tttcaggata ttttaatttg acaagcgaaa taatcatttt cagttcttcc atgttttctg
     2701 gatattctgg gtcaaatacc ttgctatgac ggccaatccc gccattgtcg atcaatcggt
     2761 aaattcgatt ccattcaatg aattggtcaa ttgattggaa attgctgatt ggatagccgc
     2821 cacaatccaa aatctctgat acacgaaatg ctaggtatgc actatcgatc actttatttt
     2881 ttttcgtgtc tatcttctca tttctttcga taagaatttt ttcaggacga tgtttcaagc
     2941 tccactctaa tagtagaaag aaagcctcca tccgatactc ttcttcaaat aaccgaaagg
     3001 ctgaatcaga acgacgaaaa agcagcggaa tcagataatt ttgtctataa atcagcattg
     3061 gtgcgcggtc caccatacac tctaaataac ggttcagata ccctgtaccg acatagtttc
     3121 ctaatcgata gccttcaacg ccgaatcttg cgtatttttg cgctaatttt gaacaatcag
     3181 ttacgttcac agctaactct tctactttgc ggtaaccgct taagatacgt tcaaaaggtt
     3241 ctaaagtgat ttttgcttcc atattcttcc ccgtccatct tccacatact tcatatcatt
     3301 tcacatgata cttatgctct catcatacca caggttgaat tagaaaagaa aataaatcac
     3361 acaaaaaagc aagacaatct gttagtgaga ttgtcttgct tttcgaaaaa gactgaatac
     3421 ttttcgttaa aactggttgc ttagtcttct aatgccacat tatggaacac atgctgtaca
     3481 tcttctagat cttccagtgc atcaatcatt ttttcaaatg tttcttgatc ttctggagat
     3541 agtgttacct cgttttgagg gatcatttgg atctcagcga ttgaaaaatc agtgattcct
     3601 ttttctttta atgcttcttg tatactatgg aaatccgcta cttcaccgta aacgatgatt
     3661 tgtccttctt cttccattac atcgcgaaca tcgatgtctt tttccattag gtattcaagg
     3721 atctcgtcag cgtcttctcc tgcaaaagca aagattgctg tattgtcaaa catgtaagaa
     3781 accgctccgg aaacacccat gttcccgcca tttttcccaa atgctgcccg tacgtctgct
     3841 gctgttcgat tcacattgtt cgtcaatgta tcaacaatca ccattgagcc gcttggtcca
     3901 aatccttcat agcgcaattc agagtatgtt tcatctcctg caccttttgc tttttcgatt
     3961 gctcggtcaa taatatgttt tggtacatta taagttttag cgcgatcaat gacgaaacgt
     4021 agtttttggt ttgcatgcgg atcaggatca cctgattttg ctgccgcgta gatttcaatc
     4081 ccaaattttg cataaatccg gctattatta gcatctttag cagttttctt tgctacgata
     4141 tttgcccatt tacgtcccat attttcactt cgctttctga tttgatagtt tcataacgca
     4201 aaaacgtcct gttccattat acttgtgaat cacgattctg tgaagctttt tttctttctt
     4261 ccttcattct tctcattttt agtcaattat ctatactcgg attgggattt gcccgaaatt
     4321 ctgttccgga atattgcctc ccaattgata gatttgatca cttctaacta acagttccgc
     4381 ttcttgtgcc tctttttttc cgattcggga ataaatagaa atctcttttt tttcttttaa
     4441 gaatcttctt ccagcatcgg taaatccaag tagtcgaaga taatttttat tttgttggac
     4501 aggaatctct tgctttttga tattcagcaa gacataacaa gataatcgtt ggatccgtgt
     4561 ccatgtatat ctttttgttt ttacctttga cacaaaatcc tcaaaactat cagcagttgc
     4621 agctgctgtt ttcatgcgcg attctattcc ttcggtcatc tgatagatat ctgtcagttc
     4681 cagcaaagta gaagataaca accgataacg caaatacggc caataagatt cccaagtctg
     4741 cagcgaacaa gttgaaagat cttcttctgt ctgaggcggc acaagtccag ctattctttt
     4801 tccttcttct aacgcatggc gaatggctgt tgcactggct atcctctgat ctgtgatgat
     4861 tttttcatga tagcctgcac ttttacgtcg gatcggatgt aattccatag ggtggacata
     4921 cttggcattt tcctttgcat aacttaatcc caagatatga tttggcgaag aaaaatcaag
     4981 cgtaatatcg ggatacagac tgcgaaaaac ctcagtcatt ttttgcggat aactatacgt
     5041 ttgatcagac agttcttgaa atgcctcttc tatcctatct tgattctctt ggacaaagcg
     5101 tccgaaactg ctgtaatcaa acgaagtctc ttcttctgtt ccaaaacaca aactgtcaca
     5161 gtccagtgtt tgcaagattt ttactgcacc tttagcaaaa tagtcagctg actgaaccgc
     5221 ccattcgacg ggaagctcca ctactaaatc cacaccattt tgtaaagctt cattcgcgcg
     5281 atgccattta tcgatgatag ccggttcgcc tctttgcaaa aaattcccgc tcattacagc
     5341 aatgacgacc tctgctcctg ttatttttcg tgccatttcc acatgatagc gatgtccatt
     5401 gtgaaaagga ttgtactcta cgataatccc gcagcttttc atgcgcatcc tccttcatag
     5461 ttatcgataa aaaagaaata gagacaagta aaaaaaggca aaaatcggaa aaaatacccg
     5521 aactatataa actattctcg caaacacaat acttctgcga cgaaaagctg cactgcaaga
     5581 gcaatgccta tatcgttcgg atatataatt tatgttgaag atttattctt ttcttgttaa
     5641 ctcgagcagg caaaaccgct agtctttgta tgctacaaag aaccagcgct ttgatttctc
     5701 tgatgctgct tcgtctgtaa aatcagaaaa tgcttcgact ttcgaaaagc cgctgttttc
     5761 cagcattctt aaataacttt ccaatggata ggtacgctct tgatgtagtt catcttcacg
     5821 aataaatcga ccagaccctt cttgttcttc aacgaaaaag gtaagaaaat gttcgatgct
     5881 gtgtttctcg cttcctggat aactatccca caaaaaagca aaattatccc cttgataatg
     5941 atagctatat tctggaaaaa cctcatctac ttgataggtg gagtgtacgt caaataaaaa
     6001 tcggccattt tcttctaacg cttgatagac ttcatcaaac acttgctgta cctcttgtct
     6061 atttttcata tagcacagcg aatcagaaaa acaggtaatt gcctgatatt gtccgatttc
     6121 tgataaatcg agcatattgc cttccacaaa ttggatcgat gcgtcttctt catatgctcg
     6181 ctggctggca atcatcaaca tctcttctga aaggtctaac cctgtcactt tatatccatc
     6241 ttttgcaaag gcaaccgcta atgctccagt accgcatgcc agttccaaaa tattttttgt
     6301 gtcttcttgt aaatgacgtt tcgaaaaagc taaccattgc tgataaagtg tttcgtccat
     6361 cacttcatcg taaatgaaag caaatgtttc ataagccata tgttaatcca accattcact
     6421 aagatcgact agcggagcat ctgaccatag tttttccaga ttatagaaat ttcgttctgg
     6481 cgcatggaaa acatgtacga tcacatctcc aagatcgatc aatacccatt ttccgccttc
     6541 ttttccttca atacgtttta cttcatattt gttttcttct tctttatcaa tgatttcttc
     6601 tgtaattgcg ttgatttgac gttcgctatt tgctgaacag atcatgaaat aatctgccaa
     6661 caaggaaaca tttcgaacgt ctaaagcgat aatgtcttct gcccgttttg aatctgctgc
     6721 ttcaatcgca atttttaatt gttctttact atctatgatg atttcctcct aatatgagca
     6781 gcttattttt ttgctaccca gtaattatat gtttcgattg tttttggata aatctgttgt
     6841 tcttgcgcaa gcaaatgggc caatgtatgt tttgtttcaa atgctacagc ttcgtctaga
     6901 tcagcccatg caatggctcg tgcatcttgt accccaggaa aatctcgacc tggttcaata
     6961 tagtctgcta cataaatgat tttgtcaagg agactcatct tagcagcccc agtcgtatgc
     7021 acccggattg catgaagaat ctctgcatcg gtgattccta attcccgctc tacaaacgaa
     7081 gcgccaacaa gaccatgcca gattgcatta ttataattca ataactccgg atcaaaaccg
     7141 tctcttacga ttaccatttt gaactcctca tctggtcgtt cttttgcata atcatgtgtc
     7201 aatgcagcaa tactagcttt ttcaggagaa gcaccgtatt ttttggcaag agccacggct
     7261 gtttcttcta ctccaagcac atgcttgaag cgttgttcgc tcattctcat ctgtattttt
     7321 tgcatcaggt tttctctaga ataagcagta tattcattac tatagtccat attcatacaa
     7381 cccttcattt tgaatataat caattacttt atctggaaca aggtagcgga tagaacagcc
     7441 ttctttgatc ttttgacgta ttttcgtgga actgatgtcg atctcaggta catctaccca
     7501 aattacagga tatggagtat ccgttgtgta tcctggtcga cgtataccca caaagttgac
     7561 catagaagtc agctcatcga ttttatacca ttttggcaaa tattctacca tatccccacc
     7621 gataataaag taataatctg tatccggatt gttttgcgtc aattccttca tagtatcata
     7681 ggtgtaactt ttcccaccac gagctaattc aatcgtttca atctgcaaaa aaggattgtc
     7741 ttccaccgct aattccaaca tgttgagtcg atgttttgca tcaatcgtct gtttctcatc
     7801 aacatgaggt ggctgataag aaggcatcaa aaatacgcgg tcaagaccta aattccggcc
     7861 agcttgttca gccatcacta aatgtgcaag gtgaacggga ttgaatgttc cgcccaagat
     7921 ccctacttgc tttttcttct ctaagaattg aggcatttct tcaggaaata attgtgaacg
     7981 aacaaatgtc ttagcttgcg tattcatcat ggtcactcct tatattgctt ttacttcttt
     8041 tgatagcctt tgatactttt cttttgtgga aggtttgtat aatacaataa cacgtccaat
     8101 gatttggacg atttcgcaat ctatttcttt ttctaatact tgagctgctt cttctgcaat
     8161 ctcatctgta ttttgaagta acgatacttt gatcagttct cttttttcta aagcttcttc
     8221 aatttgaacg atcattgctg aattgatccc accttttccg atttgaaaaa tcggctgaag
     8281 atgatgcgct ttgctacgta aaaaacgctt ttgttttcct cttaaactca taattttcct
     8341 tcttccttaa atcaatgctt tgcgtttcaa tacatctaca ccctttggcg cccagcccgc
     8401 aattactact ggcgctgtta cagtgatcca gcctagaccg gcaaaaacga tgtctgtttt
     8461 ttctttcaca gaaaactcaa aacgaacaag ctccggaaat gcctcgactt catcaggacg
     8521 aggaggctgt aataatccac ctacatgttt ttgataaaat tcgtctgctt tttccagttt
     8581 cgttcgatga atatttacat catttgacac ataagcaata aatgaagcac gattgccgct
     8641 gataaaatca aatcttgcaa gtccgcccaa gaaaagtgtt tgcccttcat tcagctgata
     8701 aactttaggt ttgatttctt ttaacggtgc aatgatccgc aaatcttttt tacctaagta
     8761 gtgcgccatt tgatggcgat ggataattcc cggtgtatca atcaaaaaat gcccgtcatc
     8821 taaaggaatc tcgattttat ccaaggtcgt tcctggaaac tgcgaagttg tgattacatc
     8881 ttgtactccc gctgtatttt tgatgatttg attgataagc gtcgattttc cgacatttgt
     8941 tactccgacg atatagacat cccgtccatc gcggtattct tcgatttttt ctaatagttc
     9001 cgtcatctca tgagatttct tagcactagt taataataca tctactggac gaattcctgc
     9061 ttcgtaagca cgttccttca tccattgtgt taatttagtt tttttcaatg atttaggtaa
     9121 tatatccact ttgttcccaa ctaaaagtac tggattgtca ccgacaaaac ggtgtagtcc
     9181 tgggattagt gaaccattga agtcaaaaat atccaccaca ttgacgatca atgcatcttt
     9241 ttgccctaat tcattcagta agcgcaaaaa gtcgtcatcc gtcaatgaga catcttggat
     9301 ctcattataa tggcgtaatc tgaaacaacg ctgacaatag acttccccgg aagctaaacc
     9361 tttttccaaa gccgtttgcg gcgtgtatcc taattcaccg ggtttgtttg tttgtataac
     9421 ggcaccacag ccgatacatt gtaattcttc tgtcattcga ttccacctcg ccagcccata
     9481 tcagggtgtt ttgctaatag ataggccata attctttttt cccaaaaacg attaaattgt
     9541 gtcttccaac tatctgttgc gataatgggt tttacaagga tgctacgtat ccctgcacga
     9601 ttggctccac gtatatcagt cataatctga tcgcctacca ttactatctc atcatctgct
     9661 aaatccaatt gtttctttgc tatgttgatc cctcttgcta aaggtttcat tgctcgtgca
     9721 acatacaaaa gatcaaattt ttcaatcgct cgcgcgacac gttttgaatt attgtttgaa
     9781 acaacaacaa cagggattcc tgcatttttc atttctaaaa tccaagtaag caactcttct
     9841 gttccatctg gattattcca tgcaatcaac gtattgtcta aatcggtcaa tactgctttg
     9901 atgcctagat tctttaattg ggcaggtgta atctgataga tggcttctac catccatgtt
     9961 ggtttgtaat ttgaaaacat gctgtctcct catctgcaaa aaatgaagga gcacataaag
    10021 tactcctcaa ccccgtatta tactgcatat cagcaaaaaa aactacctct atactcgttc
    10081 gtctttcaga aaaagcattt ctttacaaaa tgaccaaaaa taaagattga tcacagaaaa
    10141 atttagaaac aaaaaacaac taatattggc ggaacctctt gtatcgtttt tccagtaatt
    10201 tatgctgatc taatcgaaca agttccgtca atctttcaat caacgctttt ttcataatgt
    10261 gtgcgacttt atatcgtggc aactgctcgc cgttgaacgt ttcaggaata actcgatcaa
    10321 cgattcctaa ctcgcgtagt tctggcgcag taattttcat caactcagct gcttctgcgg
    10381 cgcgactacc gtctttccac aaaatcgacg caaatccttc tggagaaagc acagcataaa
    10441 tcgtttcttc cagcatccag acttcatccg ctaaacctag agcaagggca ccgccacttc
    10501 caccttcacc aatgatgatg gcgatgatcg gtacctttaa atcagacatt tcgagcagat
    10561 ttctggcaat agcctctcct tcgccgcgct cttctgctcc tacaccgcaa tatgcacctg
    10621 ctgtattgat caaggtaatc accggtctat tgaacttttc cgcttgtttc atcagtcgta
    10681 aagcttttcg atagccttca ggatggggag aaccaaaatt tctgcggata ttttcctgca
    10741 gatcccgtcc tttttgaata ccgatcactg ttaccggttt gccatctaat cgagcgattc
    10801 caccaacaat cgcttcgtca tctgcaaata aacgatcacc atggcattcc tcgaaagaat
    10861 cgaagatctc ttcaaaaaaa tccaagcttg tccatcgttc tttagctctt gctaactgaa
    10921 cgacatcatg tgctgtctta tccacgatct atccatcctt tctgtgtatg caattcaaga
    10981 agcgtatgga ttttctgttt caattccatg cggggaacaa tctgatcgac aaaaccatga
    11041 gataataaga attcagcctt ttggaaatct tctggaagtt cctgcttgat cgtctgttcg
    11101 atgacgcgtc ttccagcaaa accgatcaag ctttggggtt ctgctagaat aatatcgcct
    11161 tccattgcaa aactggcagt tactcctccg gtcgtcgggt ccgttaaaac agttagataa
    11221 aaaagacctt cattgctatg ccgtttgaca gcagcagaga tttttgccat ctgcattaga
    11281 gaaaagattc cttcttgcat ccgtgcgcca cctgaagctg tgaacaagac gactggaaga
    11341 ctttcctttg ttgctcgttc gaataatctg gtgatctttt ctccgacaat cgttcccata
    11401 cttcccatga taaaattagg atccatcact ccaataatga agggaatccc ctgaatggtt
    11461 gctttaccag tgaggactgc ttcatgaagt cctgttttat cttgcatttt tttaattttt
    11521 tccgtatatt cgggaaatgc tagcggatct ttggttacaa gatccgtatc ccattcttca
    11581 aaactttttt catcaataat caaagctaga cgctgccatg caccgattcg aaaagcgtat
    11641 ccgcaatgag ggcatacttt ttcttcaccg atgtctttcg tataaaggat atgtttgcaa
    11701 tttggacact ttgcccacat gttatcagga acagatggcg agttagcagg tttgctctct
    11761 ctattaggat tgatccgaat atatttcttt ttcttaaaca gagccatcta tgcgcctcct
    11821 atctccgttt ctggtgtcca attcggtaaa aattcttctt gtaaaaatgc tgtgctgtaa
    11881 tcacctgcta tgactgcagg atgactaatc aaatccatct gaaattctgc gttcgtgatg
    11941 attccttctg tgataaattc acttaaggct cgctgcatct tcattaatgc ttcaaaacgc
    12001 gtatttccat ggacgattat ttttgcaatc attgaatcat aatatggcgg gatcgtatat
    12061 cccgggtaaa ctgcactgtc tactcgcagt cccatcccac ctgctggaag cagtaagttt
    12121 tggatcttcc ctggagaagg agcaaaatgg aaagcaggat tttctgcatt aatccggcat
    12181 tcgattgcgt gtccttggaa tactacttct tcttgacaca cattcaaagg ctcaccggct
    12241 gctatctcca cctgtttttt tactaaatcg attcctgtga ccatttctgt tactggatgt
    12301 tccacttgga ttcgagtatt catttccatg aaataaaaat cgccttcttc atccatcaga
    12361 aactcgatcg ttccggcatt ttcataattg actgctttag ctgctcgaac agcagcgtct
    12421 cctagtgctt ctctttttgt ttgtgagatc actacagaag gagattcttc cagtactttt
    12481 tgattgttgc gttgtaaaga acaatctcgc tcgcctaaat gaatgacatg accaaatcga
    12541 tcacctaaga tctgaacttc tatatggcga gcaggataaa tgattttttc caaatacata
    12601 tcatcatttc caaatgccgc tgctgcttct tgctgagctg aagaaaaatg ctgtgggagc
    12661 tcttcttttg attgaacttt ccgaatgcct ttcccgccac cacctgcagc tgctttcagc
    12721 atcactggat aaccaattct gtctgctatt tccaatgctt cttctactgt agataataca
    12781 ccagtacttc ctggaatgac tgggacattt gcttcttgca tcaattgacg cgcattgatc
    12841 ttatttccca ttgcatcgat cgttgcactt ttaggaccaa taaatgtgat attgcactct
    12901 tcacacatgg aggcaaatcg gctattttca gataaaaaac caaatcctgg atgaattgct
    12961 tcggcttttg tcacgattgc agcgctcagt acttcctgaa cattcagata tgaatcaacg
    13021 gcttttgccg ggccaatgca gattgcttca tctgctaact gtgtatgaag tgcttcggca
    13081 tctgcttctg aatatatcgc tacggtttgg atccctagtt cgcggcatgc acgaatgata
    13141 cgtacagcga tttcaccgcg gttagctatc aatactttag aaaacatatt tcacctatcc
    13201 aatcataaag gttaattccg cttcggctac ttttttaccg tctactttag cgattccttt
    13261 gcctattccg gctgaagctt tcactttcaa tatctcaact tcaaggtata aggtatctcc
    13321 cggtgtaact ttcttacgga attttgcttt gtccaatccg ccgaaataag ccgtctttcc
    13381 cttgaattgt tcaagagaaa gtaatgcaac tgctcctgct tgagccattg cttcaacaat
    13441 caatacccct ggcataacag gttcttctgg aaaatgtcct tgaaaaaagg gctcattgat
    13501 cgttacattt ttcttagcaa cgatccgttc tccttcaacc atttcttcca ctcgatcaat
    13561 caaaagcatt ggataacgat gtggaatgat ttcttttatt tcttgtatgt tcattgtttt
    13621 ccctctttcc tgctttgctt atttttgctg aatcctaaag agtggctgtc catattcgac
    13681 aactgactcg ttttctacaa ggatttctgt gatcactccg tcggcctcac tagttatctc
    13741 attcatcagt ttcatcgctt caataatgca gataacttct cctttggcaa cttgatcacc
    13801 tacttttttg aaagctggtt tgtcgggcgc tggttgcaga taaacgattc ccacgattgg
    13861 cgaaacaacc tcgattccat caaatactgc cgattctttt tctttcatta tttctggagc
    13921 agttgttgct tgttcagata tgacattgac tggctccgaa actggcaacg tttcgtttgt
    13981 ttgaaccata ttcatgcctc gacttgtttt atttttattc atatacaatt caaattgacc
    14041 ttcacgcaaa tcgaattccg ttagcgagga ttgatcaaat tgactgacta attctctgat
    14101 ttcattgata tccaaaatta ttcctcccag cgtttcatgc acagaaccgc attatgtccg
    14161 ccaaacccta atgaattact cattgcataa gtaaaacttg caggtttgct ttcgtttttg
    14221 acaactgtca agtcaatctc aggatcttgt ttttctacat tgatcgtagg tgggataaat
    14281 tgatgttcta atgccagcaa agttgccaag gcttcgatcc cgccggctgc tcccaataaa
    14341 tgtccagtca tcgatttcgt gctgctgacg taagtatctc tgaaattttc acctaatgcg
    14401 atttggattg cttttgcttc tgcctggtca ttggctggtg tacttgttcc gtgagcattg
    14461 atatatccga cttgttccgg ctgtacacca gcttcttcca tcgcaagctt cattgcttta
    14521 gatgcaccag aaccatctgg agtaggagct gtcatatggt aagcgtcaca gtttgcaccg
    14581 tatccaacga tttctccata gattttggca ccgcgagctt gtgcatgttc taaagattca
    14641 agaactagaa cgcctgctcc ttcacccatc acaaaaccat tccgattttc gtcaaacgga
    14701 atcgaaccac gattaggatc agttgattct gagagtgctg ttaaagctgc aaatccagca
    14761 atccccatct cacaaatcgt tgcttctgtt cctccagcaa gaatgacatc agaatagcca
    14821 tgtttgatgt tacggaatgc ttccccaatt gaattcgttc cgctagcaca agctgttacg
    14881 atagctgtac aaataccttt tgcacctaca cgcaaagcaa tatttccagc tgccatgttg
    14941 gcaatcgcca ttggtacgaa cattggagaa actcgttttg cacctttttc atgtagacga
    15001 ataacttggt tttcgattgt tggaagacca ccgatcccag atccgatgat gacgccaaac
    15061 cggtcttgat tcattttttc tttatctaaa ccgctcatct ccattgcttc taaagcagcg
    15121 tagattccat agatcgaaaa catatccatt cgtttgctat cttttttaat aaaatattta
    15181 tcgtaaggga aatctttgac ttcacccgct aaggtaatcc ctgtttctga agcatcaaat
    15241 ttagtgatcg gtgcgatgcc attcgtcccc tcttttaagc tattcaaaaa gctgtctgcg
    15301 tcatttccaa taggtgaagt gactccatag ccagtaatta ccactcgatt catttgtttg
    15361 ctctcctttt cttatccgtg catgaccaat ccgccatcga cattgatgac ttgtccggtg
    15421 atatatggat tttgtgctaa gaaaactgct gtttgtgcaa tatcttctac ttgtccgaaa
    15481 cgctgcatcg gaatttgctt ttccgcttgt tccttgactt tgtctgctaa aacttccgtc
    15541 atatcagttg tgatgaatcc tggcgcaatg gcattacatg taatgcctct agttgcagct
    15601 tctcgtgcca cagattttgt taatcctact acgccagctt tactagctgc ataatttgct
    15661 tgaccaatat ttccaatcaa cccagataca ctagagagat taatgatggc cccttctcgc
    15721 tgcttcatca tttttttcaa tacgtgctgc gtcatattga aggttccaat caaattgata
    15781 tccagacatt ttttgaaatc ttctgcatcc attcgcatca caagtttgtc atttgtgatt
    15841 ccggcattgt ttactaacac atggatcgat ccaagctttt cttctgcttc tttgatcatt
    15901 tgtcccgctt tctcataatc agagatgtca ccggaaactc ctacgcattt tacaccgaat
    15961 gcttcgattt cttctatctt ttcttttgga atctctccgc gaccattcaa aatgatattt
    16021 gctccagctt ttgcaaaagc taaggccatc gctttgccga ttcctcttgt actacctgtg
    16081 ataaatacat tttttccttt tacatccata acgttcctcc ttatagtatt gctattgttt
    16141 catcaaatgt ctgcttattc tcaacacgca gaactggtat tgttttatcg atttttttca
    16201 taaatccgct taatactttt ccggggccga cttcgatgac ttgttcgatg ccgattgttt
    16261 ttaacttatg gatactctca tagaaacgaa caggttgcat gacttgctgt tcaagtagtc
    16321 ccgcgattgt ttctttcttc attatttccg ttgttgtgtt gcttatgata ggaaaagaag
    16381 gttcagagaa ttgaatctga cttaggtcct tcgccagttt ttttgctgcc ggttctaaaa
    16441 tagctgtatg aaaaggcccg ctgacattta gagggatcat tcttttgaac cctttttctt
    16501 ttaatagtaa aacagcttcg tctactgctt tttcttcgcc accaatcacg atctgctgag
    16561 gtgtattata atttgcagga gatacgatgc cgtacttgct agcttcatga cagctttcct
    16621 cgatcgtttc aatcggcgca ttcatcactg caaccatttt cccgcttcca gctggggtag
    16681 cttctgtcat ataagcgcct ctttttgcta ctaacgcaac tgcttcagtg aaggatagcg
    16741 cattactagc tactaatgcc gaatattctc ctaaactcag tcctaaagct gcatctggga
    16801 tgattccatg ctccttcaat aagcgataat acgcgatgct gactgttaga atagctggct
    16861 gtgtatattg cgtctgatcc aaacgttcat tttctgtaaa gcaaagttca gccatatcgt
    16921 agccgagaat ctcacttgct tcatcaaatg tctgctttac aacggcttct tcatataaat
    16981 cttttcccat accttgatat tgtgcacctt ggccactaaa taagaatgct gttttcatgt
    17041 ttttctccaa tatatccaga atatatgttt gattcttcct attcaaaaag gccgagacat
    17101 tcctatttca ggaaatgtcc cagcccccaa cctaatttta atgattatgc agtttgtttt
    17161 tctacgtaag tcactaaatc ttgaaccgtt ttgattccgt cttctgtttc gattttcacg
    17221 tcaaattcat cttcaatttc attgatgatt tggaataaat ccaagctgtc agcttctaag
    17281 tcttcttgga tgtcagtagt caatttgatt tcttcagttt cttttcctaa ttcttctgcg
    17341 atgatttctt gaattttttc gaataccata ataatatatt cctccgatat tgtttgcttt
    17401 ttatttttat aatttaacag tctacaactc catgagaata cttccccatg ttaaacctcc
    17461 gccatagcct gtaaatatta ctttctgctg ttttcctaag gtgatctttc cactagaaac
    17521 tgcttcatcc aataagatag ggatgcttgc agcagatgta ttcccatatt tatccatatt
    17581 ggtcaggaac ttttcttgcg gcatctttac ttttcgggca attttctcaa taattctgag
    17641 gtttgcctga tgcaaaagca gataatcaac tggtttgtct ttcatgactt cttggatatt
    17701 gtttgcaacg tctcgtacgg caaaatcaaa aatatctctg ccagtcattt gcaaataata
    17761 cgaaccttct gaatcttctg aatagaacgg atttttattt tgctggtagc cagcggtcaa
    17821 tgagctactt ctggtaccgt cagctgctaa tttttctttc agcaattttt tctgtggatt
    17881 cgcttctaag agaatcccac ctgctccatc gccaaacaac acagcagtcg tacgatcaga
    17941 ccaatcgatt agcttagata gtgtttcacc accaatcacc agtcccttag ttgagccgca
    18001 gcggatcatt ttttcagcca tgcttaacgc ataaacaaat cctgagcacg cagcactgat
    18061 atcaaaagcg atagcatgag ttgctcccaa tttcccttgt acttgtgctg ctacagatgg
    18121 tgtcgtgtaa tcgggcgaca ttgttgctac taaaataaaa tccaaatctt tagggtttag
    18181 atttttctgt tttatcaatt tttccgccac taaaatgcat aaatcagaag tctcctgatt
    18241 tgttacacat cttcgttcac gaatacccgt ccttgacgaa atccattcat cagaggtatc
    18301 catcatcttg gaaagatcat tattagtaat aatcttttca ggaaggacgc tggctgttgc
    18361 agtaatcatt ccaaattgtt ccattaaata tcctcactta tagtcttgca agaagtcgtg
    18421 caagtttttc agtgcatcca gcaaggcatg ctgttcctcc gtagccattc cgtctaagat
    18481 gttcttgacc atttcacgat gaaaatgctg atggacccga aatagtaact ttcctttttt
    18541 cgttagtccc aactttacaa cgcggcggtc atcttcactg cggatacgtt cgacatagcc
    18601 tttttttact aacttgttaa tagcagtggt caaggtccca acagtaattg atagttcttt
    18661 ggctacttct gaagttgttt ttttcttgta catcccgatt gcctcgatcg tgtgcatctc
    18721 tgtgattgac agatctttga attgagattt tttcagctca gactcttcga ttgtcaaaat
    18781 atcattgaag acactaacaa gataatcatt tactgttttt aaatctggtt ccatgttcca
    18841 ccgtccacta attagtttga ttatcaaatc ttttgatgtt caaactatat cttaactttt
    18901 ttcaaaatgc aaccttaaaa gatcattttt ttactatatg agcttttttt aagattttac
    18961 tcatttaaaa caactgataa atcagcattt ataatttctt ttattagaaa aatcatctat
    19021 ctcacatatt ttgattatca aagtattttc tgtgaacaga actatagaat tccctaaaac
    19081 ttgtccatta tttcaaaatt tgttatagtc tttttattca aagcgtaaaa ggagatccta
    19141 tgttaaaaga aaaaatgaaa actctcccca tgctgccggg tgtctatctt atgaaagacc
    19201 gagatggtaa ggtcatctat gttggtaaag caaaacggct caaaaataga gtaagcagct
    19261 attttcatca gaataaacag cattcaaaaa aagtattacg tatgattcat catataacag
    19321 attttgattt cgtcgtagta gacacggaat tagatgcttt attgctcgag tgtcaattga
    19381 tccaacatta ccgtcctttt tataaccgac aaatgaatta tttcagtaat tataattatg
    19441 tacatattac caataaggga tttgtattaa cagatactcc tacggctcga acatacgggc
    19501 cttttcgtct ctataaaaaa atgccgtcta ttttgcgtat aatggaagaa acttatcaaa
    19561 tgccttggtt gtctgaaatt agtttgttag ctttgcgagt ccagcttcct gacctacagg
    19621 aaatgacttt tgaacagaaa aaaaaggaac tccaagggct gtttcaaggc cgaaataaaa
    19681 agctactgac ttacttgaaa aaaagacaac aacatttcat ctatcagttg aatttcgaaa
    19741 aagccggtat gcttcaaaaa gatatcgaat tagttactta ttttattaga cggatccagg
    19801 aacaaaagca gtttttaaga acaccatcgc tcaccttcag catgccatta gcagcagatg
    19861 agagtcagaa aaagcactac ctcgtttgtt atggtcaact tgcagaaaca atgattacct
    19921 ctggtgacgt ttctcctgac ttctattatg aaaagaaaga agctcattta tctttgaaac
    19981 gtcaattgtc aaaagaagaa atcgatcctg tacaaatctt gatcagttac cgcaaaaaat
    20041 tagaaaaaga acagtttgaa attgaactgt taaacaaaaa agaagctgaa aagcaactaa
    20101 attagttgct tctcggcttc ttttaggcat cttcaacttc aaatgtcaat tatcctaaaa
    20161 tttattggat taagtcatag atttccatcg ctacgatatc gatattatca aattcgtatt
    20221 gctgatgagt atctgtttca gttaattcaa atgtatcggt cgctttatca taagttactg
    20281 tacacttctc tacgccttct ttttcaaaac ggcgaacttg cagttcatta tctgctgact
    20341 cgaccatagc ctccagtcgt tttatgattg cgactaattg tgaatgtgtc atcggaggtc
    20401 ctccttttct tatttctgtt atattgtatc aaaagcatag cctacttgca ttgattttct
    20461 ctaaaaaaac tcgtttttta tgattttttt tctggtattt ttccttcagg ccaccaaagt
    20521 ttgatcaaag cttgggttcc gtcattctca aaacctttat ctgccaactc ttcatatagt
    20581 ttcaatgctt gggtagttgc tggcaataca agatccattt tttctgcttc ttctaatgca
    20641 atctttaaat ctttgataaa atgtttgaca aaaaaaccag gtgtgtaatc ttcttttaaa
    20701 atacgaggag aataattgct taaagaccag ttagctgcac ttccacctga aagcgtctca
    20761 atcactttct ttaaatcaag tccgtttttt tgtgcataga caagcatttc cgtcattcct
    20821 gtcatcgtac ctgcaatcat gatctgatta gccatttttg tatgctgacc tttcccagca
    20881 gaaccatgca atgtaaatgt cgtaccaaat tctttgaata aaggcagtac ttgatcataa
    20941 acggcttctt ctccaccgac catgatcgtc agtgtaccat tctttgctcc taagtcacca
    21001 ccggataccg gtgcatctaa cgcatctgcg cctttttctt ttgctgtttt tgcaattttt
    21061 tctgccaggc ttggtgtact tgtagtcaaa tcaactaaaa tatgtcctga gatatctgca
    21121 gaaaaaatcc ccgactgtcc atagtagatt tcttctacat ctttggggta acctaccatt
    21181 gtaaagagaa tattactttg ttctgctacc gcttttggtg tatctgccca gatagctcct
    21241 tctgttacta aaggatcagc ttttgctttt gtccgattat agacagctac ttcgtgtcct
    21301 gcttctagta gatggcgtgc gacagcactt cccatgacgc ctgttccgat aaaaccaagt
    21361 ttcatttttt cgacctccta aaaatcaaaa atccttgctt ctatgctagt atacgctaga
    21421 aacaaggatt ttttcataaa aaatgtttga attttatctt ttaaatgaat ttaattttat
    21481 tttgattcgt tcaatcgagt cttcttcttc ttttcttcgc atctcttctt catgataaac
    21541 aaccatgtct tgataaaatt cgatcatagc ttcgccgaaa tgtgtagatg aaatatcata
    21601 aagtttagct tctaaaactt ttggatcttt aggaacttca ctggaaatat aatcaataag
    21661 tgctggtgca aagtcttcgt cggtttcaaa agttttccct aatgaaggat gatcgaacaa
    21721 tcggttcaaa taagcatttc cttccacgac acaaggtgtt ccggctgcca tcgcttctgt
    21781 ataagtaagc ccttgtgttt ctgaagtaga agcactgaca aaataatctg ccgctttata
    21841 ataaagtgct acatcattgt tgtccacttc tcctgtgaac tgcacaaatt cttctaactg
    21901 cagttctgag atcaattcct ctaactcttc tacataaggt ccttttccta cgataactaa
    21961 tctggcgtta ggataagcgg agatgacctg aggaaaccca ttaatcaagg cttggatatt
    22021 tttctcataa gaaatcctgc ttaaagaaag aatcatcaaa tggttttcct gaagacctag
    22081 tgactccctt aatttgctga tatcctgctg agtgatatca gaacgtttga atttttcaat
    22141 gtcgatacca gtaggaatca cgcgaagcgg tgctattaca ccatagctgc gtaatgtatc
    22201 aataacacgt tcacttggac aaactacacc tgtcgtatga tttgcaaata atctagacaa
    22261 gtattttaca tgggattgcc tgatcacttt tcctttaccg atataatgaa ggtagtcttc
    22321 atacatcgta tggtaagtat ggatcagagg tattttcaac ttcttagcta ccatcttccc
    22381 aagtatccct gctccgaatt ctgtatgggt atgaataaga tctagttcta gttctttggc
    22441 aaccaaataa gcataccaca tgcctctaac aacaactctt cgatccttga aagaaataaa
    22501 tggtacactt ggcattcgaa tcacatcttt ttccaactta tccgcattag gatcggtcgt
    22561 tgtaaaaata tagacttcat gcccatgtct ttctaattct tgttttaatg ttttgatcga
    22621 tgtagcaact ccgctgactt gcggaaagta ggtatcggta aaaaagccga ttttcatgtt
    22681 tttcgcctcc aaactagtcc ttgacctgat ttactttttt taatttgcta gagacttttt
    22741 cattcagtac actttcgtat acatttttaa gttcttcacc gattttattg atacttcgct
    22801 cttttgctgt ttgataaccg gcttgtgtcg tactcggtag ttcttgattg gtaatctgtt
    22861 ggatcagtcg gataaattct tcgtttgtct tccccatata gcagttcttg ctatcttcta
    22921 accagccatt atacactgga atatctcgaa caagtacgtt ttgtttgctt gctaaagctt
    22981 ctaaaacaac gatcccttct gtctcttcat aagaagggaa aaagaataga tctgcacctg
    23041 aatacgcacc ttctatgatc tctccacgaa tgtaaccagg aaacaggaca ttttcaggat
    23101 gatcttcttt tacaattttc cggatgttcc gaggaatcga gtacatggga acatgaccaa
    23161 accaaataaa tgtgtattcg ggcatcttct tagcaatttc tacgaaatca atgattcctt
    23221 tacgttcgaa aaacaaaccg acacaaatga ttactttttg atccggagac agtttgaaat
    23281 attctctgaa cttttgttct ttcattggat cagggtgata tttctctaag tcgatcccat
    23341 tggaaatagc ttgaatcggc acacggatac catagctttc caacaactgt tttgaataag
    23401 gtgttggcgt gatcagatag tccgcttttt ggtacaatcc taccagatat ttttttacga
    23461 taggagaaag ctggttcgat ccgataaagg aatttcggaa atcttcctct gtagaatgcg
    23521 catgatagac aacttttttt ccttctcttc tggctttatt gaccatatta tgactattga
    23581 ttccgtatgt attgatgtgc aggatgtcat aatcttcttt ttcatctagt gtataagaga
    23641 taccgacttc cgtcagagca cgtttttgat gatcaagcgc acgcccgatt ccagattttg
    23701 caagcatctt ttcactttca aaatataata ataccttcac cttacgtccc ccttcactta
    23761 tatagtataa catagcctac aaactgtcac tcaaacttaa gccctatttt tgtctattct
    23821 tactgacaaa aacgttttct taaaaaagaa caaaaaaact ggcgtagctc ttttacaagc
    23881 cacaccagct tttgatacgt tgtttatttt acatattcat gaacaagttc aacgacttct
    23941 tccatcatat cgcattcttt caatgcacga tcagcaagtt cagccatttt cgctgtgtcc
    24001 aagcgtttca ttaaactacg cgttttaaga atagatgtcg cactcattga gaactcatct
    24061 agacccatac ctactagcaa tggaacagct gtttgatctc cagccatttc tccacacata
    24121 cctgcccatt ttccttcagc gtgtgctgca tcaattacat ttttgatcaa acgtaaaatt
    24181 gatgggttat atggttggta caagtaagaa acgcgttcgt tcatacggtc tgctgccatt
    24241 gtgtattgaa tcaaatcgtt cgtacctaca gagaagaaat ctacttcttt tgcaaacttg
    24301 tctgctaaga ctgctgctgc tggaatctcg atcatgatgc cgacttggat cgtatcagat
    24361 acttccttgc cttcacttat caatttttgt ttttcttctt caaagatcgc ttttgctgcg
    24421 cggaattctt tgagtgtagc taccataggg aacatgatac gtaaattacc atgaacagat
    24481 gcacgaagca atgcacgcat ttgtgtacgg aacatttcgt cgccttgttc agataaactg
    24541 atacgcaatg cacgatatcc taagaatgga ttcatttcat gaggtaattg cagatatggt
    24601 agttctttat ctccaccgat gtccattgta cgaacaacta caggttttcc ttccatacct
    24661 tctaatacag ctttgtaagc ttcatattgg tcatcttctg ttgggaagtc tggagaatcc
    24721 atatacagga attcagtacg gtacagacca actgcttctc caccattatt atggacacca
    24781 actagatctt ttggtgttcc gatgtttgca gctaattcaa aatgcttgcc gtcagctgtg
    24841 acagtttctg cattttttaa tttttcccat tctgcttttt gtgctgcgta atctgcacct
    24901 gctttttcaa attcagcttt ttgttcatct gtaggatcga tgattacatc gccttcgatc
    24961 ccgttgacag ccaaaatatc gccttctttg actttagcag taatttcttt tgtcccaacg
    25021 attgctggaa tttctaaaga acgtgccatg atagcagaat gtgaagtacg tccaccaatg
    25081 tctgttacaa acgcttttac aaaattgcga tctaattgtg ctgtatcact tggagtcaag
    25141 tcatgtgcaa caacgaccac ttcttcgttg atcattgatg gatttggtaa agtaacgcct
    25201 aataagtgcg ctaaaatacg tttagcaacg tcacgaatat ctgcagcacg ctcttgcata
    25261 taagcattat cttccattgc ttcgaacata ccgatataca tatccgttac ttcttttagc
    25321 gctgattcag catttacgct gttgtcttta atattttgct tgatttgacc taccatttct
    25381 ggatctgaaa gaaccatcaa atgggcatca aatacttgcg cttctgcttc acctaagctt
    25441 tgcgctgctt tttcacgaat ttgctgcaat tcttcagtag atttagctaa tgcagcatcc
    25501 aaacgagctt cttcagccgc agtatcttct actgttgcct tactgaatgt taaatccggt
    25561 tgaactagca ggtaagcttt tgcaacggct acaccgtcac tagcggcaat cccttttagc
    25621 atttcaacca ttattcagat aagccttctt tcttcattgt ttcaacgatt gctgccattg
    25681 catcagcttc gtcagcacct tctgcagtaa tagtaacatc agaaccttga cctacaccta
    25741 aagacataac gcccatgatt gatttcaagt ttactgattt acctttatac tctaagttta
    25801 catcagagtt aaatttgctt gctgtttgta ctaatagtgt agctggacga gcgtggatcc
    25861 cagtttctgc tactacgtga aattctttct tttccatatt aatcggtctc ctttaagtga
    25921 gtgttgtttt tatgtgaggg ttttcttttt tagtaattca gagaatccta acaaatgcca
    25981 ccctttcatc tgtaaagatt aacatttttt ttcgttagat acaacagttt tcattcaatt
    26041 tcagataaaa gtattcaaat tattccggat tctttttcat ccttttcttc acattttata
    26101 acaaacgtgt ttatttcctt tttcttgtcg ctttctcttc tcctgacgtt tatcattctt
    26161 tcctttgacc ctctttgttt tcatatgaaa cgaattactt gcttcccttt ttatttttag
    26221 tataataaga gtcaaaggtc aggaatggtc aatagagatt tcaccttttc tacctcataa
    26281 atctcttaaa aaactagggg ttattctttt acctcatgaa aaacgagatg taaaatatgg
    26341 atgtacccat ttttgtgaaa ggacgtgaat gattcatgct ttgtcaaaac tgcggcaaga
    26401 atgaggcaac gattcacttg tatgccactg tcaatggtca gcggacacaa ctcgattact
    26461 gtcagagctg ttatcaaaaa atcaaaaatc aacaaaatgg aggtctgact aatatggccc
    26521 aaaatgatcc atttggattt ggcagtttgg acgatctttt ccgttccatg tctcgccaaa
    26581 tgcaacagca aggaaattat gagcaaacac caccaacaca atttggcgga aacaacaact
    26641 tcaatggtgg tcagccacct caaggcaata cagaaggttt attgggagaa tacggaatca
    26701 atatcacaga acaagcacga caaggtgata tcgatcctgt gatcggacgc gatgacgaaa
    26761 tcaaacgagt cattgaaatt ttaaatcgcc gaaccaagaa caatcctgtc ttgattggag
    26821 aaccaggtgt tggtaaaaca gcagttgttg aaggtttagc acaaaaaatc gtagatggtg
    26881 atgtaccgca aaaattaatg gacaaagaag tcattcgttt agatgtcgta tctttagtcc
    26941 aaggtactgg tatacgagga caatttgaag aacgtatgca aaaattgatt gaagagatcc
    27001 gtcaagcaga aaatgtgatc ctattcatag atgaagttca cgaaatcgtc ggtgctggtt
    27061 ctgctggtga tggaaatatg gatgctggta atattttaaa accagcttta gctcgaggag
    27121 aattgcaaat ggttggtgct actaccttaa atgagtaccg catcatcgaa aaagacgctg
    27181 ctttagaacg tcgtatgcaa cctgtacggg tagatgaacc aactgtcgaa gaaacgatca
    27241 gtattttgaa aggtttgcaa aaacgttatg aagactatca tcatgtaaaa tatactgatg
    27301 aggccatcga agcagccgca actttatcca atcgttacgt ccaagatcgc ttcttgccgg
    27361 ataaagccat cgatttgcta gatgaaacag gttctaagaa aaacttgacg atccaaatcg
    27421 ttgatcctaa aacaatcgag aaaaaactgc aagaagcaga agaacaaaaa gttttagctt
    27481 ctcgagaaga agatttcgaa aaagcagctt attatcgcga tcagatcaat aaacttcaaa
    27541 aaatgaaaga acgtcagctc actgaagagg aaacacctgt gattaccgaa aaagatatgg
    27601 aaaaaatcgt tgaacaacgg acaggtattc cagtgggtga attaaaagaa aaagaacaga
    27661 cacaactgaa aaacttagca gatgacttga aagctcatgt catcggacaa gataacgctg
    27721 tagatcgagt agcgaaagcg attcgccgaa accgtgttgg attaaacaaa cagaatcgtc
    27781 caatcggctc cttcttattt gttggaccaa ctggtgtagg taaaacagag cttgcgaaac
    27841 aacttgccta cgaactattc ggttcacaag attcaatgat tcgtttcgat atgtctgaat
    27901 atatggaaaa acatagtgtc tctaaattga ttggttctcc tccaggttat gtcggatacg
    27961 aagaagctgg tcaattaaca gaaaaagtac ggagaaatcc ttacagtctt gtcttgttag
    28021 atgaagtaga aaaagctcat ccagatgttt tgcatatgtt cttgcaaatc ttagatgatg
    28081 gtcgattgac agatgcacaa ggaagaacgg tcagctttaa agatacgatc atcattatga
    28141 caagtaatgc cggaactggt aaagtagaag caaacgttgg ttttggtgcg gcacgcgaag
    28201 gtgttactcg ttctgtattg aaccaattaa acaactactt tacaccagaa tttctgaacc
    28261 gttttgacgg gatcatcgaa ttcagtgcat tatcaaaaga aaacttgatg acaatcgtca
    28321 cactaatgct agacgatgtc aaccaaatgc tggcagcaca gcaacttcac atcgaagttc
    28381 ctacgaatgt caaagaaaaa ttagttgact taggctatga tccatctatg ggtgctcgtc
    28441 ctcttcgacg cacgatccaa gaacaaatcg aagatggtat cgctgaattt tatcttgatc
    28501 acccttctat ccatgagttg aaagcaaaac tggataaaga tggaaaaatc atcgttactt
    28561 ctaaaccaga acgtttggct aaagaatcag ccgaagaaac ggctgaataa atccagtttt
    28621 atagcgtgag acggtatttc tttgagaaat gccgtctcat tcttttttca gaaaagtcaa
    28681 aatactgttc attttataaa aaagaaatta taataagaga aacaagcaat taaaaataca
    28741 taaaagaggt gagcgccatg aaattggtaa atgtaacaaa cagccattcc cgtcttgtat
    28801 taaaccagct tgaaaacact gatgcccatc tagtcaaggt ctacacagcc ggaaatacca
    28861 ctattgtcta cactgaagcc cctgaacata atgaaattct gctgatcaat gacaaacgta
    28921 aaatccagcc gaaagaaatc gaagatgcta aaaattattt cttagataaa atcaaagacg
    28981 ccgtttacca cgaggatgaa atcaaagtaa tcgaattggc aggccttgtt gaaatctcga
    29041 ttccaaaaca tgaattaggc gctacattag ccgtgtaaac aaattttgaa cataataatt
    29101 aaagagcgtg ggacaaaa
//
LOCUS       DAFEPQ010000029        28948 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.029, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000029
VERSION     DAFEPQ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.029"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            131..505
                     /locus_tag="LP543_001419"
     CDS             131..505
                     /locus_tag="LP543_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL2321592.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="LP543_001420"
     CDS             complement(619..1248)
                     /locus_tag="LP543_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL2321593.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLPLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="LP543_001421"
     tRNA            complement(1382..1454)
                     /locus_tag="LP543_001421"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="LP543_001422"
     tRNA            1668..1741
                     /locus_tag="LP543_001422"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="LP543_001423"
     CDS             complement(1879..2043)
                     /locus_tag="LP543_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321594.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="LP543_001424"
     CDS             2338..3666
                     /locus_tag="LP543_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBL2321595.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="LP543_001425"
     CDS             3868..4701
                     /locus_tag="LP543_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBL2321596.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="LP543_001426"
     CDS             4717..5454
                     /locus_tag="LP543_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBL2321597.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="LP543_001427"
     CDS             complement(5609..6577)
                     /locus_tag="LP543_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBL2321598.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="LP543_001428"
     CDS             6809..7642
                     /locus_tag="LP543_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBL2321599.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="LP543_001429"
     CDS             7676..8515
                     /locus_tag="LP543_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL2321600.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="LP543_001430"
     CDS             8541..9101
                     /locus_tag="LP543_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL2321601.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="LP543_001431"
     CDS             9300..11021
                     /locus_tag="LP543_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBL2321602.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="LP543_001432"
     CDS             11186..12328
                     /locus_tag="LP543_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL2321603.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="LP543_001433"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="LP543_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBL2321604.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..14775
                     /locus_tag="LP543_001434"
     CDS             14128..14775
                     /locus_tag="LP543_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBL2321605.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    14904..15120
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15168..17813
                     /locus_tag="LP543_001435"
     CDS             15168..17813
                     /locus_tag="LP543_001435"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBL2321606.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18123..19436
                     /locus_tag="LP543_001436"
     CDS             18123..19436
                     /locus_tag="LP543_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBL2321607.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19426..20079
                     /locus_tag="LP543_001437"
     CDS             19426..20079
                     /locus_tag="LP543_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL2321608.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20134..20805
                     /gene="radC"
                     /locus_tag="LP543_001438"
     CDS             20134..20805
                     /gene="radC"
                     /locus_tag="LP543_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBL2321609.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(20700..22052)
                     /locus_tag="LP543_001439"
     CDS             complement(20700..22052)
                     /locus_tag="LP543_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2321610.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
     gene            complement(22118..23407)
                     /locus_tag="LP543_001440"
     CDS             complement(22118..23407)
                     /locus_tag="LP543_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBL2321611.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(23494..24198)
                     /locus_tag="LP543_001441"
     CDS             complement(23494..24198)
                     /locus_tag="LP543_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HBL2321612.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            24398..24664
                     /locus_tag="LP543_001442"
     CDS             24398..24664
                     /locus_tag="LP543_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2321613.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            24677..24835
                     /locus_tag="LP543_001443"
     CDS             24677..24835
                     /locus_tag="LP543_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321614.1"
                     /translation="MEEKNIKVPTLEEYTKIVVETDEQNPKTIAVITANDIESCEGFR
                     VRMTPRYD"
     gene            complement(24832..25062)
                     /locus_tag="LP543_001444"
     CDS             complement(24832..25062)
                     /locus_tag="LP543_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBL2321615.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            25134..25289
                     /locus_tag="LP543_001445"
     CDS             25134..25289
                     /locus_tag="LP543_001445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321616.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            25446..25601
                     /locus_tag="LP543_001446"
     CDS             25446..25601
                     /locus_tag="LP543_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321617.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGEKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(25598..25822)
                     /locus_tag="LP543_001447"
     CDS             complement(25598..25822)
                     /locus_tag="LP543_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBL2321618.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            26089..26424
                     /locus_tag="LP543_001448"
     CDS             26089..26424
                     /locus_tag="LP543_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321619.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            26657..27598
                     /locus_tag="LP543_001449"
     CDS             26657..27598
                     /locus_tag="LP543_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBL2321620.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            27600..28490
                     /locus_tag="LP543_001450"
     CDS             27600..28490
                     /locus_tag="LP543_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBL2321621.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            28533..>28948
                     /locus_tag="LP543_001451"
     CDS             28533..>28948
                     /locus_tag="LP543_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBL2321622.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEK"
BASE COUNT         9724 a         4812 c         5742 g         8670 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt aggggaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcaga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccggattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
    14341 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
    14401 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
    14461 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
    14521 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
    14581 atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat
    14641 gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt
    14701 caatacattg atttgacaac cgagttgcag acgcttattt actttgatga gaattactca
    14761 gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat
    14821 tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa
    14881 aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc
    14941 attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt
    15001 gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag
    15061 aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa
    15121 tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa
    15181 ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt
    15241 tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa
    15301 tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc
    15361 aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta
    15421 tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga
    15481 tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag
    15541 agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta
    15601 gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct
    15661 tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac
    15721 gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata
    15781 tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa
    15841 cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca
    15901 tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat
    15961 atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat gatccaaacg
    16021 actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta
    16081 cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa
    16141 ttgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg
    16201 tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg
    16261 taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg
    16321 aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt
    16381 gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa
    16441 caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc
    16501 ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc
    16561 cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt
    16621 atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg
    16681 aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga
    16741 tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag
    16801 atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct
    16861 atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg
    16921 ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag
    16981 tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat
    17041 ttgatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt
    17101 tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa
    17161 aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc
    17221 caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc
    17281 tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag
    17341 ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata
    17401 caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat
    17461 ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat
    17521 caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct
    17581 tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac
    17641 tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg
    17701 tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag
    17761 agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga
    17821 aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca
    17881 ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg
    17941 tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta
    18001 tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc
    18061 atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt
    18121 tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc
    18181 ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa
    18241 aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc
    18301 tattagaaga ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca
    18361 atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa
    18421 aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg
    18481 aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag
    18541 cgattatcga agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc
    18601 tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag
    18661 aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata
    18721 ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt
    18781 atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag
    18841 tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc
    18901 aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg
    18961 aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag
    19021 gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc
    19081 atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca
    19141 ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag
    19201 taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg
    19261 aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag
    19321 cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt
    19381 tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca
    19441 gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca
    19501 caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta
    19561 ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa
    19621 acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa
    19681 ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag
    19741 attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag
    19801 aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa
    19861 ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca
    19921 ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta
    19981 gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt
    20041 gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc
    20101 ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta
    20161 cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat
    20221 caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt
    20281 gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat
    20341 gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc
    20401 gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc
    20461 tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt
    20521 tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg
    20581 ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca
    20641 gcaaggatcg ttttagcgca taatcatcct tccggaatat ataagttagt acacataaat
    20701 tatataagga aatttactgt tatattgcca tctggaaata ctattatgtt atcgataatt
    20761 gaacgccata atgcattttt ttcttcttcc gaagattctt tataaatttc atcgaattct
    20821 tttgatagaa taaattcgac attttttata tctattttag ctggctctac ttcaatttca
    20881 tttaattctt tcattaaagc ttctcgatct tttttgtatt cctctaagtc tataacttca
    20941 ttcaagtagg ctttcttcaa tctatctaat ttattctcaa tatttttctt actatttttc
    21001 tttttaataa tctttggttg tttttttgaa tagtttattt gtatttgttc aaatttttgt
    21061 ttagtagctt caaaaatata acgttctaaa tttttttcgt aaaaagcgcg cttatttgtg
    21121 caacgtaatt tattatttct tcgttttaca catctataac aagtcctttc gtatatagat
    21181 tctgtaccgt cttttttctt atatcttgct ttaatagttt gaccagctac tgatccctga
    21241 cagtcagaac attttaataa acctttaaaa atgtaatcac gttttgcatt tgtttttaaa
    21301 tttttaggta aggctttttg aacagcataa aaaagttttt tatcaataat aggaggacag
    21361 taatttttat tatttcttaa ttcaccaatg tattttttgt tcgtcaacat atttttaaca
    21421 ctttgataat ctctaacgat tccatactga gagcctaaat aataaacagt ttttcttaaa
    21481 ctaccactag aaagaaagta gttgaatata tctatcacga taggagcatc atcatttatt
    21541 actaatttct tattttctat agagtagcct aacggagttt tcccgcttac tacttcacct
    21601 tgtgcaattt tatttgccat aacagctttg atgcgttctg aatcaatttg ggcttccaat
    21661 tcagcaaaac tcattacttg tgcaatgaaa gttctgccat acgctgtagt tgtatcataa
    21721 tattgttgag aaacagcatt ccaagaaaca ttgtgttttt ctaaaatttc ttgagtgttt
    21781 aaataatgtc gtagatttct aaaccatcta tctaattttg taaataaaat tagatctatt
    21841 ttattttctt ttacatcttg taatagtttt tgaaattcat cacgatttat tttttgacca
    21901 gaaataccac cgtctacata ttcattaact actttcaaat ccttgtgttg ttcgatatat
    21961 tcatagaggg tttctttttg ttctctaaga ctatctccat gtttggcttg ttgttcagtt
    22021 gatactctca tatataatgc tactcgtttc atgttatctc ctttcgaatg tatgttctta
    22081 tcgttgtaaa agaaaagccc gaaggctaat ctttttacta cacatctgtt aattctaatg
    22141 atcttctgta ttgttcagct tgagctagtt tagtaaaaga tacagttccg ttataatttt
    22201 ctaaaacaac tttttcaatt tcatcaagag atactttaaa gaattctttt cgttgattaa
    22261 ctttattgac ttcgtttttt ctaaaatgcg aatgtagttg attttctaat gttggtgcat
    22321 cttctgaaaa tatcattgca tgaacatcaa attcaaaagg tacagaagcg ctactaagtt
    22381 ccttaattct atccataggt tctaaccgtc tagtcattcc tattttatag acatcttcac
    22441 cgaaagatcc gatattactg atgacataaa catagccagc tctagtatta agttctcttt
    22501 gcaatacatc ttctttgaca gtttctagtt ctcttaattt ctcttccagt tctttaattt
    22561 tatcagcata gagttctttt tcaacatcag cactagcttt ttgcatgtat gccattaact
    22621 tagttatctc tgagttaaac tgtcgttctt ctttatcaat gcgtttcttt tcacgttcta
    22681 tttcacgtct taccttttct tcttcaatca tttgttcttt tattgctttt tgctgttctt
    22741 tttcatcgtt aactttaact atgtatgaat agttgagttc taattcttga agttttaatt
    22801 caagtaattc ttttttgaat tctgcttcat cgtttttaaa aagcttgttt attttatcaa
    22861 aagttcttat aattttttct ctagttgaat ctacgttaga aatagtaagt ttattgagaa
    22921 gtccagttat ttctgattct aatggtataa gcattttctt tttaatatta ttagcttctc
    22981 tattattcaa ataagaagga atattaattg caccgtctaa aataaaatcc ttttctttaa
    23041 tctttaataa agaaagtttg ttttttattt cggatgaaga agtttcatct atattcggaa
    23101 taattactga tttatagata gtgaccagag atgtgtcata tttttgatta atttcatcgt
    23161 ctaaccgctg gctttcagca ttaagaatat caattttgtt ttctagcgtt gacaagtttt
    23221 tattcaattt acttatgttt ttctcttttt cagatataaa cgtttcccta gcatttaatt
    23281 cttgagaata ttcctttacc ttactctcta aggcagaagt tttatctttt aattttttag
    23341 cttcagataa tttgctacta cccataatta ttaaaactat tccaactaaa aacaatagta
    23401 ctcccattca aaacgcctcg cttcttttat aaaataattg tcaaaatcat ataaaccctt
    23461 accgaagttg acggctccgg catgggaatt tttttaaaat acaacttttc cgataattct
    23521 aatctgttct ggcgatacaa ttctatcttc gtataagtcg tttatagaac gtaaaatgat
    23581 tgtttgattt tcgtaatctt tatatatctc tttacatgta actccgtcac cacctaattc
    23641 cacaatagca atttccccat cttctacgtc cggttgttct ttaacgaata cgaattgtcc
    23701 attcctgata acaggttcca ttgaatcgcc tataataggt actgcgaagt cagcagcact
    23761 tggaactctc tcaaattctt cctcttcgac aaccgtatca ccgtaagtaa gttctgttgg
    23821 attggcagcc gaagccgaat tgtgaacagt tgggattttt tcttttttag gaaaatctag
    23881 gacattttct tgtttttgtt cttctaatcg attacttgca aattcatagg tagcaacttt
    23941 attttcgtcg ttaagctgat tatatatagg aaggatatta tatatctgat tagatataga
    24001 taaatcaata ttcatcaaat catctatatc aacattgaat atcttagcta tctcatttaa
    24061 ggtttttaat tttggtgtat attttccttt ttcccattcg cttattgagg atgaactttt
    24121 tcttcctagt ttttcggcta gttcttgttg atcaatatta tacttttctc taagaaactt
    24181 tagattttca gcgaacatag taacacctcc ttgagttaat tgtaacaccg tttcggaaaa
    24241 aatgtaacag gtttcttaga aaatatttta gtgtttttaa agaagtattt acgcaaaata
    24301 gaaatcgatt tcaggaaaac tgaaatatgc acttgacttc ggaaaaaccg aagtatataa
    24361 tttagtcaga aagttaaaga gaggaggcga ataaaatatg gataccgcga agaaagaaaa
    24421 atatacattg agacaattaa gagctttaaa gggttattca aaagaagaac tgtcaaggaa
    24481 aagcaaagtg acatcaagaa caatatttat ttacgaaaac gatgtagata aaatgaggaa
    24541 cggaaagtac gccactctag aaaaaattgc taaggcattg ggtgttagag tttccgatat
    24601 ttttttagac cctgattcgg aaaaaccgaa atcaataaca ggtgaaacaa aacaagtcag
    24661 ctaggaggtg tgaatagtgg aagagaagaa tataaaagta ccgactcttg aagaatatac
    24721 aaaaattgtt gtcgaaacag acgagcagaa cccgaaaaca atcgcagtta ttacggcgaa
    24781 tgatattgaa tcatgcgaag gttttcgagt tcgaatgacc cctagatatg attaatgttc
    24841 tttatcacgt ggaggtatag gatcgtttcc aaaactatct tttgattgta tttgtccatt
    24901 ctttttttgg acaataaact ctgattgctg ttttttagaa atctttttac catactcaac
    24961 agcatctgat ttattatcaa atagttttgt atcttcggaa ttaccttcac cacgtacagc
    25021 ccatttgcca tttctaggag atacccattg atttttaccc ataacaattc acctcgcttt
    25081 caagttaatt ctattaagag gtgaaaagaa aacaaactga tggaggtgag attgtgagta
    25141 acaagccaaa aatcactttg aatggcacac ctaatcttca aagattagca gatgaaatgg
    25201 ctagaatacg ttctagacat tggggagtta ctgtggtggc aaccataatt gaacaaaaag
    25261 aaaagcccac acagccggca agcaagtagt gggcaccata gaacaataat attatatcac
    25321 acactcgcca cttccgaaaa gaggagttgc tgctctcctt tcaagtttgc caaagtccac
    25381 gtttcttcat tttggcaact cctcttcttg gaggtggtga cggcgagaga cgaaggaggg
    25441 atgacatgaa ggaagaatca aaaataggga ttttgaatga gaacaattac acaagattaa
    25501 ttattgaaac agaagaaggg gaaaaagtag cagaagttac attgaccgat gcaactcctg
    25561 ctgacggtta tcgtattcga ttaactccta aatatgatta accttttggt gggtgtggat
    25621 cgtttccgtg actatcacgg ctttgaattt ttccatctct gccatgaata aatagctcag
    25681 atccttgatt tttagaaatc tctctagcga tttttactgc ttctgatttt gtattagtat
    25741 gtgcagtcgc tttagaattt ccagcacctt tgacattcca accaccattg gaattaggta
    25801 caacatgttg attttttccc atagttttca cctccatttc tatagaaata ttttatcaaa
    25861 tagaaatgga gaaattcaat aaacgaaagg agggatcaaa tgacattaca agagcatcaa
    25921 cacttgatgc gaaaactaaa tcaggaatac cacgaggaaa tgaaacctag attgctaggg
    25981 gaacaatggc gtgaacgcca gaagaagtgg ttagaaataa aaaagtgact cagccgtcca
    26041 aagcaatgag tcacaaacca aaaattatac ctaaggagat tttatcacat gaataagaaa
    26101 attgaaaatc ttatcgaaga attgaaacgt gaatgccaaa agcaaggtgt ttctattatt
    26161 tgtacagccc aaaaagaagg ggaacttaaa agtctcgttt atggtgagac aacagaaatt
    26221 ttactttgtc ttgctatgca agaagaacat ttagatgaaa atctcccatt gtcagcacat
    26281 attatgcgta gaattgctgt ggatgcttac gaacaagcga aaaatgaaga agaaaatcaa
    26341 ccttcaaatc atacttttgt tattaataac aaggaagatt tagctgatgt gatgactcgt
    26401 attctcaagg gggaatttca atgatgaatg aaaaaacaat tgatctactc attattttat
    26461 ttttcacgtt agtgattgta gcgaaaagtt atgtaatctt tggcttgtta ttcgcaatta
    26521 ccatgcttgt gctttgcgtg ttagcaagca gaaaagaggt taaacatgaa cgagttagca
    26581 aatcttgaca actatttaac tgatcctgat tatgctaaac caccatatga agcgccaatt
    26641 gatgaggagg atgaagatga gtaaatctac cttagaaatg agccatcaag aatggctcga
    26701 agaccgcaaa agaggtattg gtggctcaga tgttgcaacc gtacttggat taaacaaata
    26761 caaatctcct tatcaattgt ggcttgagaa aacgggtcaa attgaattga aagatttaga
    26821 aagcgaacca gcttattggg gtaatgtttt agaagaagtg gttgctaaag agtttcagga
    26881 acgtacaggc aaaaaagtac gcagaagaaa ccaagtattt gaacatccat tacatccatt
    26941 tttaagagca aatattgatc gggaagtagt gggagaaaat gccattctgg aatgtaaaac
    27001 agccaatcaa tttctcggaa aagagtggga aggtgaagag gtaccgctta gctatctctg
    27061 tcaagttcag cattacatga acgttttaaa caaagactat tgttatatcg ctgttttgat
    27121 cggtggacaa aaattcattt ggaagcggat tgaacgagat caagagctga tcgatacaat
    27181 cactgaacaa ttagtagagt tttgggaaac gaacgttctt ggaggtatcg agcctgtaat
    27241 tgacggaagt ggagcgactg ctgacttctt aaaagaaaaa tatgcagatg tagaagaaaa
    27301 tcaaacagct ctaccatcac gttttgatga acttatcgag caaaaaagag aactcaagcg
    27361 gacgaaaaaa gaaattgaat catctattcg tcaagtagac aacgagatca tcagtgagct
    27421 aggtaaacgt gaagccagta tcggtatcac acaaagaaac attatcagct ggaaacttgt
    27481 tcgtacaaga cggatgaaca cgaagaaact agcagagaaa tatccagatg tcgcaaatga
    27541 tgaagagatt tataacgtta ctgaatcaag aaggctaacc gaaaaggaga tcaaataata
    27601 tggcaacaaa tgaatcgtta aaaaatcaat tggcagaaaa gccacagaaa caagttgcac
    27661 caggacagtt agggcttaaa gctctaatga atacaccaac aatgagaaag aaatttgaag
    27721 aagtacttca tgacaatgct aatgctttta tgtcgaatgt tatgactctt gtatctaatg
    27781 acagttatct tgcagatagt gaaccgatgt ctatcatgag tggtgcgtta actgctgcaa
    27841 cattaaatct tgggctagat aagaatttag gttatgcata tttagttcca ttcaatagta
    27901 aaaacaagca aacaggaaaa tgggaaaaga aagctcaatt catgcttggc tataaaggat
    27961 atatccaatt agcccaacga tcaggtaaat acaaagcatt aaatgtgatc gaagtttacg
    28021 aaggagaact aaaaagctgg aaccgactga cagaagagtt tgagtttgat ccaaatggta
    28081 gaacgtctga tgaagtcatt ggatatgttg gctatttcga gttactgaat ggattcaaaa
    28141 aaactgtcta ttggaccaaa caagaaatcg aagctcatcg gattgctaac aataaagatc
    28201 gcgataagac aaagttaagt ggtgtgtggg catctgatta caatgcaatg gcacgaaaaa
    28261 ctgttttgag aaatcttctt tctaaatggg gaatcttgtc catcgaaatg caagaagcca
    28321 ccacatcgga tgagagagtc caaagggttc aagaagacgg cagcattatt gctgaaacag
    28381 aagttgaaga agatattcct gaaagaaaag aagcagaggt tatatctgaa gaaaatgaag
    28441 atgtacaaac tggattattt gatgcatcta atccgccgtt aaacaaataa tgagggagct
    28501 ttctcccttg cttttctaga ggagaaatac gaatgaacac aggatatgtc aaattacatc
    28561 gaaaagtgat gaattcattc gtttggacca atccttatat gtataaatta tggagcttgt
    28621 gtttgatgaa agcgagtcat gagaatcgca agatactttt taatggaaaa gagatacaag
    28681 tgaacagcgg agaattcgtc acagggcgcg atgctatcac atctgagatg aacgaaggcg
    28741 tgaaacgtga acatcaagtg aacagcggtt ctgtatggag atggctaaaa caatttgaaa
    28801 aaaacggaat gttgaacatc aaatcaacta cgaaatacag cgttatatca ataaaaaact
    28861 ggtctttgta ccaaagcagt gaacaacaaa tgaacatcaa acgcacaaca agtgaacaac
    28921 aagtgcacac aaacaagaat gaaaagaa
//
LOCUS       DAFEPQ010000030        28828 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.030, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000030
VERSION     DAFEPQ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.030"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(43..681)
                     /gene="plsY"
                     /locus_tag="LP543_001452"
     CDS             complement(43..681)
                     /gene="plsY"
                     /locus_tag="LP543_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBL2321623.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTENRIPFGLNSSKKKS"
     gene            808..1242
                     /locus_tag="LP543_001453"
     CDS             808..1242
                     /locus_tag="LP543_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBL2321624.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1408..3453
                     /gene="parE"
                     /locus_tag="LP543_001454"
     CDS             1408..3453
                     /gene="parE"
                     /locus_tag="LP543_001454"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBL2321625.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3465..5915
                     /gene="parC"
                     /locus_tag="LP543_001455"
     CDS             3465..5915
                     /gene="parC"
                     /locus_tag="LP543_001455"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBL2321626.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6192..8423
                     /gene="pflB"
                     /locus_tag="LP543_001456"
     CDS             6192..8423
                     /gene="pflB"
                     /locus_tag="LP543_001456"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBL2321627.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8667..9428
                     /gene="pflA"
                     /locus_tag="LP543_001457"
     CDS             8667..9428
                     /gene="pflA"
                     /locus_tag="LP543_001457"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBL2321628.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9513..10439
                     /locus_tag="LP543_001458"
     CDS             9513..10439
                     /locus_tag="LP543_001458"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBL2321629.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10621..11514
                     /locus_tag="LP543_001459"
     CDS             10621..11514
                     /locus_tag="LP543_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBL2321630.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11648..11773
                     /locus_tag="LP543_001460"
     CDS             11648..11773
                     /locus_tag="LP543_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2321631.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11907..12539)
                     /locus_tag="LP543_001461"
     CDS             complement(11907..12539)
                     /locus_tag="LP543_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321632.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(12687..13229)
                     /locus_tag="LP543_001462"
     CDS             complement(12687..13229)
                     /locus_tag="LP543_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBL2321633.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13364..13753
                     /locus_tag="LP543_001463"
     CDS             13364..13753
                     /locus_tag="LP543_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321634.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13757..14521
                     /locus_tag="LP543_001464"
     CDS             13757..14521
                     /locus_tag="LP543_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL2321635.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            14650..15597
                     /locus_tag="LP543_001465"
     CDS             14650..15597
                     /locus_tag="LP543_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2321636.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            15727..16467
                     /locus_tag="LP543_001466"
     CDS             15727..16467
                     /locus_tag="LP543_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321637.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(16758..17426)
                     /locus_tag="LP543_001467"
     CDS             complement(16758..17426)
                     /locus_tag="LP543_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL2321638.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            17560..17847
                     /locus_tag="LP543_001468"
     CDS             17560..17847
                     /locus_tag="LP543_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321639.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            18263..19801
                     /locus_tag="LP543_001469"
     CDS             18263..19801
                     /locus_tag="LP543_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBL2321640.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            19798..20763
                     /locus_tag="LP543_001470"
     CDS             19798..20763
                     /locus_tag="LP543_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2321641.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            20750..22072
                     /locus_tag="LP543_001471"
     CDS             20750..22072
                     /locus_tag="LP543_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL2321642.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      22135..22239
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22313..23056
                     /locus_tag="LP543_001472"
     CDS             22313..23056
                     /locus_tag="LP543_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2321643.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            23071..24063
                     /locus_tag="LP543_001473"
     CDS             23071..24063
                     /locus_tag="LP543_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL2321644.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            24075..24812
                     /locus_tag="LP543_001474"
     CDS             24075..24812
                     /locus_tag="LP543_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2321645.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            24802..25524
                     /locus_tag="LP543_001475"
     CDS             24802..25524
                     /locus_tag="LP543_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBL2321646.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(25608..26231)
                     /locus_tag="LP543_001476"
     CDS             complement(25608..26231)
                     /locus_tag="LP543_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL2321647.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            26626..27317
                     /locus_tag="LP543_001477"
                     /pseudo
     CDS             26626..27317
                     /locus_tag="LP543_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
     gene            27525..27815
                     /locus_tag="LP543_001478"
     CDS             27525..27815
                     /locus_tag="LP543_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321648.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT        10005 a         4823 c         5949 g         8051 t
ORIGIN      
        1 atttgtcaca ctcccgtttt atctctttgg aatgaaaaag tatcaggatt ttttcttgct
       61 gctattcaaa ccaaacggga ttcgattttc agtatggttt ctgatacgct tgatattgtc
      121 tttatgccga taaaagataa aggaagaaac taaaaaggca atgatcgtca gtaaccaatt
      181 gggcttagcc aaaatcacag gagctacata aggaaggacg atcgtagata aagtgatcaa
      241 tactgcactg atcatactgc tcaaactcac catacttgta caaaataaac taacgataaa
      301 gatgatacaa gaataaacaa aaaatacagg attaaatcct aaaagcattc ctgcacttgt
      361 cgcaacagct tttcctcctt tgaacccagc aaaaatcgga aatgtatggc caagaatggc
      421 gcatacccca aaccaaagag ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt
      481 agccaatgta ccttttaaaa tatccataag caggacaaca atcccggctg ttttgcctaa
      541 tacacgaaat gtattggtcg ttccagtatt accgctcccg tgttgtcgga tatctttttt
      601 gaaaaacaat ttgccaatcc agacaccgga aggaatagaa cctaaaagat atgctaaaat
      661 aagtaataag atcaatttca ttttttaccc tctttcaagt tgaaaactat cctttatttt
      721 agcatgaata ggtaacagga acaattgaaa aaatgaaaga tacaagtata atagatggaa
      781 taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt
      841 ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa
      901 cgaacaagtt acatggttgc acaggtattg caagacaatg gatataaaat cataccagta
      961 aatccattat tggctggtga agaaattcta ggcgagaaag tctatgcgaa tttgacagaa
     1021 atcgatgatc caatcgatat cgtagatgta tttagacgga gtgaattttt acctgaaata
     1081 gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg cacaattagg gattgaaaac
     1141 aaagaagcag ctgagatgct aagaaaagca ggacgtacag atatcgtaat ggatcgttgt
     1201 attaagattg aacttggtaa gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga
     1261 gatgatcgat agaggggaat gtcgaaaaag cggttaggag ataattaaaa ttgatttctc
     1321 gtctaccctc ttttttttca cgaccttttc tagtatgata atcgtggtgc ggttttgccg
     1381 caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag taaacaacga atacaatgac
     1441 tcgtccatcc aagtattgga aggattagag gcagttagaa aaagaccagg gatgtacatt
     1501 ggatccactg acagccgagg cttgcatcat ttagtctatg aaatcgtcga taatgcagtt
     1561 gacgaagcat tatcaggtta tggcagtgag attgatgtga ccatccatga agacaatagt
     1621 ataactgttg cagatagcgg acgtgggatg cctgtaggaa tgcatgcttc cggaatacca
     1681 acagttgaag tgatttttac tgtgctgcat gctggcggaa agtttggtca aggagggtac
     1741 aaaacttctg gtggactgca cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg
     1801 ctaattgtaa cgatcgttag agatggcgta gagtatcagc agaaatttaa aaacggcggt
     1861 aaacctgacg gtactttgaa aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc
     1921 cattttttac cagatgatac aatattcagt acaacgaaat tttcatatga aattttagcc
     1981 gaacggttaa gagaatctgc ctttcttcta aaaggcgtta aaatctctct ttctgatctt
     2041 cgtggagaag aaccagtcaa ggaaattttt cattacgaag aaggaatcaa agaatttgtt
     2101 gattatctga atgaagaaaa agatacattg acaccagttg tttatttttc aggagaaaaa
     2161 gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg gttattcaga aaatgtttta
     2221 tcttttgtca ataacgttcg gacaaaagat gggggaaccc atgaagctgg tatgaaagcg
     2281 gcgatgacga aatcttataa tgaatatgcg agaaaagttg gtttgctgaa agaacgggac
     2341 aagaatctgg aaggtagtga tttcagagaa gggttagctg ctgtactttc tatccgtgta
     2401 cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa aacttggtac gccagttgct
     2461 cgtaccgtag tagataacgt catcagtgaa caaatgggct tttatttgca ggaaaatagc
     2521 gaaatgagcc agatgttggt ccgtaaagca atcaaagcca gagaagcgag agaagcagca
     2581 cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta aaaaaggcga atctcttctt
     2641 tcaggaaaat tgacacccgc acaatcccga aaccctaaga aaaatgaatt gtatttagtc
     2701 gaaggggatt ctgctggcgg atctgctaaa caaggaagag acagaaagtt ccaagcaatt
     2761 ctacctttac gaggaaaagt catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa
     2821 aatgaagaaa tcaatacgat gatctatacg attggtgcag gagtcggtcc tgagttttct
     2881 atcgaagact gtaattacga caaagtaatc atcatgaccg atgcggatac agacggcgcc
     2941 catattcaag tattactgct tactttcttt tatcgttata tgaaaccgct gattgaagct
     3001 gggaaagtat atatcgcgtt accgccttta tataaagtat ccaaaggaca aggaaagaag
     3061 caagtcattg aatatgcgtg gacagatgac gaattggcag caatgattaa aaaagtcggc
     3121 aaaggatata tgctgcagcg atataaagga cttggagaga tgaatgcgga acaattgtgg
     3181 gaaacaacga tggatccgac ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg
     3241 caagctgaac gtcgagtaac cacactgatg ggtgacaaag tagaacctcg aagaaaatgg
     3301 atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca gtatcttaga taaaaaagag
     3361 gatactgaaa tctctccttc agtttctaat gatttattag atgaagaacg agcagataaa
     3421 aatgaaaaca atcagttatt tgaagtagaa taggaggtca cctgatggaa aatcgacaag
     3481 aagtacaaga attaacttta gaagaagtga tgggtgaccg cttcggacgt tattccaaat
     3541 atattatcca agaacgtgca ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc
     3601 ggatattatt tgccatgaac aaagatggca atacgtatga caaaggattc cgtaaatcag
     3661 caaaatctgt cggaaatatc atggggaatt atcatcctca tggtgaccgc agtatttatg
     3721 aagcgatggt ccgcatgagc caagactgga agttgcggga agtactgatc gaaatgcacg
     3781 ggaataacgg gagtatggac ggtgatccac ctgctgcgat gcgttatact gaagccagat
     3841 tatccaagtt aagtggagag atgctagcag atatcgagaa agaaacagtc gatctcgtgt
     3901 ggaattttga tgatacagaa aaagaaccaa cagtccttcc agctcgatac ccaaaccttc
     3961 tcgtcaatgg atcgacaggt atatctgctg gatatgcaac agaaatcccg actcataact
     4021 tagctgaagt gatcgatggc acaatctata tgatcgatca tccacaagcg agccttgaaa
     4081 aattgatgga atatattcct ggacctgact ttccaacagg agggatcttg caaggaaaag
     4141 atgaaatcaa aaaagcttat gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa
     4201 agatagaggc aatgaagggt aataaacagc aaatcgtgat cacagaaatc ccatatgaag
     4261 taaataaagc gaccttggtg aaaaaaatgg atgagatccg tttgaataag aaaatcgatg
     4321 gaatagctga agtacgagat gaaagtgata gaacaggtct gcaaattgtc gtcgaattga
     4381 aaaaagacgc caatgctcaa ggaattttga attatttgtt caaaaacact gagctacaga
     4441 tcaattacaa tttcaatatg gtcgctatcg accatatgac tccgcatcaa gttggactga
     4501 aagacatttt aagaagttat atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat
     4561 tcgatttagc caaagctcaa aaaagacagc atatcgtaga aggattgatg aaagctttat
     4621 cgattctgga tgaagtaatc gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa
     4681 agaatttagt cgacgtcttt caatttacag aagaacaagc agaagcaatt gtcacattgc
     4741 aattataccg tttgacgaac acggatatca cagagcttca aaaggaatcc gaaagcttga
     4801 ttgcgcaaat cacagagttg aataaaatcc tttcaaatga caaagagctt ttctctgtta
     4861 tgaaaaaaga attgcgagaa gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg
     4921 aagacgaaat cgaagaaatc aaaattgata cgcaagtctt agtggcacaa gaagacgtaa
     4981 ttgtcagtgt gacgcgagaa ggctatgtga agcgaacaag ccttcgttct tatagtgctt
     5041 ccaaaccaga agaaatcggg atgcgagaag gcgactactt gctatactca ggtgaattga
     5101 gtacattgga ccatgttctt ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc
     5161 atgaactacc cgatttgaaa tggaaagatg ctggtgagca catttctcaa acaatctcca
     5221 atctgtctgt agacgaatcg attttagctg ttttcccata tcaaaaaatt caagcagaga
     5281 aaacatttgt attcatcagc aagaatggat tgatcaagca aacacgaatg acagagttcg
     5341 aaccatggcg cacctataag agccgtccgc tcagctgtat gaagctaaag gcggaagaag
     5401 atgaactgat tgctgtgtat ctgaaagaag atcagccagg cttagatgtt ttcttagtaa
     5461 cgcatcaagg aatgggtttg cgctatcctt taacagatgt tcccgttgtt ggagcaaagg
     5521 cagctggagt gaagtctatg aatttgaaag atggagatag gattatcaac gggctgcttg
     5581 tgttagcaga aggtgatacg ccgatcgtaa tcgttactca gcgaggagct gttaaacgca
     5641 tgcttgctca agaaatcagc caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg
     5701 agctgaaaaa aaatccgcac cgtattatct atatgtcgga aggaagatca aaagaaataa
     5761 ccttgatgaa tcaaaaaggg cagcagttaa cgattgaccc aactgatttc cctataggtg
     5821 atcgaacatc gaatggatca tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag
     5881 ttatcgatgc tcctgagatt ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga
     5941 cgccatctgt attaatacag gtggcgtctc tttttcattc atttttaata aatccttata
     6001 cgtttgcaaa aaaagatgaa agtgttcaca atgcttaaat gttggtatgt caacgttgat
     6061 ttaaatatat gtaaataaaa cataaaaatt gttaaataaa aaacaagaaa acccttgcaa
     6121 agatagggtg ctgtgttata ctaatcacgt aaaaactgtt acataaaaca gaaatttagg
     6181 aggtccatac aatggagcaa tggaatggat ttaaaggtac caaatggaaa gaaggcgtag
     6241 acactagaga ctttattcaa aataactaca ctgaatatcg cggtgatgac agctttttgg
     6301 aaggcccaac agaagcaaca gaaaaattat gggatgaatt gcaaaagtta aatgatatcc
     6361 aattcgagcg taacggtgtc tatgatatgg acacagaaat cgtatcaact attacttcac
     6421 acgatgcagg ctatttggat aaagatttgg aaaaaatcgt cggtctacag acagacaaac
     6481 ctttgaaaca agcattcatg ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa
     6541 gcaatggcta tacaccaaac gatgaattga cacatatttt cactgactgg cgcaaaacac
     6601 ataatcaagg tgtatttgac gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa
     6661 tcattacagg gcttccagac gcttacggtc gcggacgtat catcggtgac tatcgtcgtg
     6721 tggcattgta tggaatcgac tatttgatgg aacaaaagaa aaaagatcat gatatgactg
     6781 gttatcatac aatgagtgaa gaagtgattc gtttacgtga agaaatcact gaacaatatc
     6841 gtgcattagc gcaaatgaaa gaaatggctg cgaaatatgg ctatgatatt tctaaaccag
     6901 ctgcaaatgc aaaagaagct gtccaatggc tatacttcgg atatttagca gcaatcaaat
     6961 cacaaaatgg tgctgcgatg tcaatcggac gtatctcagc attccttgat atctatatcc
     7021 aacgtgattt ggataatgga gtaatcacag aaaaagaagc acaagaattg atcgaccact
     7081 tagtaatgaa attacgtatg gtcaaattcg cgcgtacacc tgaatataac caattattct
     7141 caggattccc tatctgggct actttatcca ttgcaggtat gggattagat ggtcgttcat
     7201 tagttacaaa gaatgatttc cgtctgcttc atacattaac aaatatggga ccttcaccag
     7261 aaccaaactt gactgttttg tattctgaac accttccaga aggattcaga acatatgcta
     7321 gcagaatcgc tatcgaaagt tcttcgattc aattcgaaaa tgataatcag cttcgtgaac
     7381 aatggggttc tgatgattgt gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag
     7441 acatgcaatt ctttggcgct cgtgctaact tggctaaaac actattatat gccatcaatg
     7501 gcggagtcga tgaagtgact ggcgcccaag ttggaccaga atatcgtcca atcacatcag
     7561 aaatactaga ctttgaagaa gtgaaacata acttcatgaa tatgatggat tggttagcag
     7621 aactatatgt caatacgttg aacatcattc attatatgca tgataaatat tcttatgaag
     7681 cagctcagct ggctttgatg gattctgact tgcaacgcac atttgctaca ggtattgctg
     7741 ggatctctca tgcggcagac agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc
     7801 gtaatgaaaa aggaatagca gttgattttg aagtggatgg cgacttccct aaatacggaa
     7861 atgatgatga ccgtgcagat gaattagttt catggatctt ggaatacttc atgggacaaa
     7921 tcaaacgtca taaaacttac cgcaactcaa aaccaacaac ttcattattg acgattactt
     7981 caaatgttgt ttacggtaaa aatacaggaa atactcctga tggccgtgaa gctggaaaac
     8041 cacttgcgcc aggagcaaat ccaagttaca atgcggaaca aagtgggtta ttagctagcc
     8101 tgaactcaac agctaaaatg aattacggct gtgcattgga tggaatttca aatacccaaa
     8161 caatcaatcc ttcaggtctt ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg
     8221 tcttagatgg atacttctca aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg
     8281 atttattgcg tgatgcaatg gaaaatccaa tgaaatatcc gaacttgacg atccgggtat
     8341 ctggttatgc agttaaattc cgtgacttga caagagaaca acaattagat gtcttgatga
     8401 gaacttcaca cgacagaatg taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa
     8461 aggacgttga atagccatcg catcaaaatc gaataatcag tatgcttatt tgaaagaaac
     8521 gatttacagg ggcaggactt gtatgttccg tccctgtaaa ttttacacat aacgaagcgg
     8581 caattgcgaa aaattttgtt cagaaagctt cgcttcgaca gctaacttgt acttaaaatg
     8641 aaatcaaaga aaagatgtga acttaaatgg aagaaaaaac aatcggctat gttcattcta
     8701 tcgaaacttt cggttcagta gacggacctg gactgcgctt tgtcgtgttt atgcaaggat
     8761 gccgcatgcg ttgccaattt tgtcacaatc cagatacttg gaatattggc ggagggaaag
     8821 aatacacagc agatgaactt ttagataaag cagaacgctt cagaccttac tggggagata
     8881 aaggtggtat tactgttagt ggtggagaac ctctattgca gatcgatttt ttgatcgaac
     8941 tatttaaaaa agcaaaagag cggaagatgc atactacatt ggatacatgc ggaaaacctt
     9001 ttacgtatga agaacctttc ttttcacgtt ttcaagaatt gatgaagtat acagatttac
     9061 tgttatttga catcaaacac atcgataatg aagagcataa aaaactaacg cattgggaca
     9121 atgaaaatat tttagaaatg gctcaatatt tgtcaaaaat caacaaaccg gtttggattc
     9181 gccatgtact agttcctgag cgaagtgatt atgatgaata tttgatccga ctagacaact
     9241 ttattcaaac cttgtcaaat gtagacagag tagagattct tccttatcat acgatgggaa
     9301 aatacaagtg ggaaacactt ggtttgaaat atccattaga agggatcgaa ccacctacaa
     9361 aagaacgtgt cgaaaatgct aaacgattgc ttcatacaga agagtataca aaatatttaa
     9421 cgcgataggt cgcatcggaa agaagataag tattgcttct ttccgttttt ttgctattat
     9481 taaataggag aaatcacaaa ggagcgaatg acatgtcaaa aattttagtt tttggtcacc
     9541 aaaatccaga tacagatgca atcggggcag ccattgcgtt ttcacacttg caaaatgaat
     9601 taggtaaaga aacagaagca gttgctctag gtacacctaa tgaagaaaca caatacgcat
     9661 tagaccactt tcagctatct gcaccacgag tagtagagtc agctaaagca gaaaatgcag
     9721 atgtcatgct tgtggatcat aatgaattcc aacaaagtat ttcagatatc agcgaagtaa
     9781 atattttagc tgtagtagac caccatcgta ttgcaaactt tgaaacagct aatccattgt
     9841 actatcgtgc agaaccagtt ggatgtacaa gtacgattat tttaaaacta tataaagaaa
     9901 accaagtaac aattcctaaa gaaatcgcag gaatcatgct ctcagctatc atttcagata
     9961 cattgttgtt caaatcacca acatgtacgc ctgaagatga agcagctgca aaagaacttg
    10021 cagcaatcgc tgaagtcgat ctaaacactt atggacttga aatgttgaaa gcaggaacaa
    10081 atctaagtga taaatcagct gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg
    10141 gcagttccaa cgttcgtatc gctcaagtca atacagtgga ccttaatgaa gtgatggatc
    10201 gacaagcaga gctagaagca gcaatggaag atgaaatgaa agcaaatggc tacgatctgt
    10261 tcgtattagt tgtcacaaac atcttagaca gtgattcaga attacttgtt gctggtaaag
    10321 gaaaagaaaa agtagaagaa gctttccaaa caactttgac caacaaccgt gctttgctta
    10381 aaggtgtcgt ttcacgtaaa aaacaagtcg tacctcaatt gacggctgta tttagctaat
    10441 caaatggtag tatgaagcat tcaaaatgaa tggataagcg attgatataa aaagaaacat
    10501 agaacatact aaaggccgaa gaaatggagg agaataacag tcaattatga ctcttgttct
    10561 tcctcctttt tacgtaaaag ccttttaagt aaaataaaac ggatagaaaa gagaacatat
    10621 atgaactatt attcaaaaaa taaggaaaac ttttatcaaa aactgaccga tgatccgctt
    10681 ttttcttcat tgaccgatta tctatatgaa catcgagaac aagaaacgat tctaagagaa
    10741 ttgaaaaaag aattttcgca aaataaattt tcccattttc ttgatttatt gatcgatgca
    10801 ggattaatca agagggaaga acgaagatat catttgaact ttccaatatt tgatcctaaa
    10861 gattacttgc aacaagccac ttcggctgct gaaaccatag ctgaacaatt gaagagactc
    10921 tctgttgatg aacaaaaatt agcgatggga gaagttattt gggcgtattg tttcgaagat
    10981 gaacggaaag aagcgtattt ttatggagta agaaacagcc gagagacaga attgctgagg
    11041 acaactgcag gaaatgagaa gtaccgtttt atcacacttt cctctataga acacttcccg
    11101 cttactttgg ctaattactt ttttgtacag aagaatcagc ttcctgtaac gaaggcattc
    11161 aaagaattag ccgaacttat tggagacgta aatgaatcgt acttttttga tcaaatagaa
    11221 gttatcgttg acaggatacg taagaataaa tataaaaatc gccggcctag tattttccat
    11281 cagtctctac ttgtgacgaa tactataaag gaagaagaat cattcacatt agaattacct
    11341 attgtcgaaa aaaacaattt tgagattgaa ttaccaacat tggatccttc attgacaatg
    11401 gaggaaatgg cctttcttaa acgacaaatc ttttctgagc tgagcaagaa atttataccg
    11461 catgcatttt cttatatcaa agaatatgga acagttttag tgagtaaaac ctaagagagc
    11521 agtaagcaaa aattatactg actcttgcag gaggagcctt tctcttcctt tatggttaaa
    11581 agaattattt gagtattttg cctatctacc acctattaga ttcactacat ggtctggaaa
    11641 caaaggaatg caaaaaaatg gtgagaaatg tggaatgaca aaggaagtag tgataagaaa
    11701 agttagattt ttaaataatc aatattatga ttcagtcaag tatggcattt tatgggaaga
    11761 actagctgat tagattagga agtccatttg ataatccaga atcactaaga aacgaatggc
    11821 gagtcattaa taatttatcc aatcttgggt tattctttta gattgaagtg tttaaaacta
    11881 aaaaagcatt tatgaagtcg taatagtcag gcttcaaaat gctttttatt ttgtttgttt
    11941 tttctttgcc acataaatac acctagactg acgatgaaga gcatatccaa cagatttcct
    12001 gcgacttgga tcgtcgaagg gttggtcagt ggcattaaag caaggacaaa aaaggaaaac
    12061 aagctgtaaa taaaacaggt gacaggaact aatcgaaaga agagcggaca tttcaattgg
    12121 ctaaaaatga gtgaaaaaat aaaataagta aaaatggata aaatcacgac taaaccattc
    12181 atgataaaga agaaaagcgt attgtgatta aacgaataaa aaagaattgc taaaaaagaa
    12241 aacgttttaa atgcaatcaa tacgcttgaa gcttcgttta acgtaccagg atgtcgatga
    12301 aaaaaatagg caccgaaata aacagaaaac agtaagccgc aaagagaaga caaaatcaaa
    12361 aaaaacggat tttcttcggc ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat
    12421 gcactagaag gcgcatgtga ggtcttaagg tatgaataac atagaaaaaa taagatcgtc
    12481 aaagttgcaa aaaaagagcc taaatgtaaa aaattagtgg ttctacgttg aatttccata
    12541 atatctccaa attaaattga taagtatata aagtataata acaaattgat gagtatatgt
    12601 cgatgttttt taatcaaaat aataaataaa aactgtctaa catttaaaat ttaatttaga
    12661 tattagacag tttttaagtt tgtgagttat tttccttgtc gttttctcac ttttttagtc
    12721 cagaaaccac ctgaaatata ttttggctca taagaaatga taaaagcacg ctcttcgatt
    12781 tctttgatca ggcgatacag ttcacgttcg ctttttctag gagaaagtat ttcgagaact
    12841 agccgctcac cttccagccc cattgcagaa gtctgtgtca caccataacc gtgttctctc
    12901 agtatacccg gcaaactttt atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg
    12961 atgtatccta atgccaattt gtcttcaatc aaaattccga cactgatacc tactgcgtaa
    13021 cctaacgcat agacaaataa attcagtgga ttatctaaac gatctaaaac aagggataag
    13081 ccgacgatat aaatcgtgat ctcgaccata ctgacaagtg gagcaatcag tcgatagcct
    13141 ttcattgtca acatgaaacg taacgtgttc agagtgatat aagcaaaatt aatgaggaag
    13201 atcatcccca acatttttgg atcaatcatt tgtaaaactc ctttcattta tataacaaca
    13261 atatcaaaaa aaatcgtaag tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata
    13321 aaatgattat aatagaatta taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg
    13381 tgtgttttgt gccagccacg tgctaagtct ccttctcagt atatacttag gtgtctattc
    13441 gattcaacag ccaattgagg tagagaacat tgtttttcag atgtcgctga cagatggttt
    13501 aattttaagc gtttttttct ttttgatact gtttattggt aatattttcg gtagcgttgc
    13561 cagttttttg aatttttcta tttatccact cctttcgcta gcattaggag tggtgggact
    13621 ttgttctctg tgcttatttc ccgagccatt cagtcctgca tttatattat ttggtacact
    13681 gaacctgttc cagacaactg ttggagcatg gttgctatgg cgatcaggaa atttaatgaa
    13741 aataggtgag taacaattga atccaattat attgaaacgt tctgtccatc atattccagt
    13801 gttagaagtt gttctgaaag aattaagaga ggaacgtatt cccgttgtca tctattatca
    13861 cggatggcag acaagtaagg agttagtttt gacgcaagga agaaaaattg cgcagcaggg
    13921 cattcgtgtg atcctgccag atgcgatgaa tcatggggag cgcaaacagc cggtttcttc
    13981 tattccatcc tatacatttt gggatagtat ctacggaaat ctgtttgaat ttgacacact
    14041 tatcaagcat ttagaaaaaa gaggtcttct acatgatcag cttgcagttg gcggggtttc
    14101 gatgggggga atgacaacat gcgccttgtt ggctaaacat cctcatatag tagcgggcgt
    14161 gtgtttgatg ggttcccctg cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc
    14221 ggaatataat tatgtcgtac caaaagacta ttttgatttg atttcatggg ttaagagcta
    14281 tgatttgtca ttgcagccgg aaaagttagc tggacgtcct ctgttctttt ggcatggaca
    14341 agctgacgaa aaaatccctt atatacaaac ggaacgattt gtagaaaaaa atcggcataa
    14401 gtcttacgga aaagaaattg tttttcgttc ctcaaaaaca cagcgacata tggtacaaat
    14461 accactaatg gaagaagctt ccactttttt agcaaaaaat ttgataaaaa agtataaata
    14521 attgaaacgg aaaatcactc atgacgatag aggattttcc gttttttatt ttgggctata
    14581 ataaactctg ttaggaaaat ttggtataat tgatagtaag aatgacaaca tgttagaaag
    14641 gaagagagca tgcccacttt agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg
    14701 gtctctcgag tcatcaatca tccggaacag gtgactgacg aattgaaaga gttagttttc
    14761 tcagcaatgg ctgaactgga ttaccgtcca aatattgctg caaaagcact tgtcacgaat
    14821 cggtcgcaga tcatcaaatt atttatactg gaagaaatcg acacaactga gccctattac
    14881 atgaatttgc tgatgggaat cgctaaacgg atcggtaaag cgcattattc tctccaatta
    14941 gttacaaatg atgccttcga cgtcggttct tgtgatggat acatcatcac aggtgtcaga
    15001 aaaaaagact tcgattggat cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat
    15061 tctgaaggat atgattatgt agacagcgat aatgaatatg gtacagcaaa agcaacgcaa
    15121 tatgccttag aaagagatta tcaaacgatt atttatatcg gtatcgaagt ggacgagcct
    15181 tttgaaattt caagggaaac tggttattta tctgtgatga aggaagttga tcttcctagt
    15241 aaaatcttgc ggtttccaaa tcattcgaca gaagtagaaa gatatatgac agaaaactgg
    15301 gcagatttta cagataaaac ttgttttgta tgtagttcag atcggctagc actaggagca
    15361 gtaagaggga ttataaatgc tggaggacac ttgcccgaag aatttggtgt gattggattt
    15421 gatggggtat ttttagacca aatcagttca cctaaattga caacgatcaa gcaagatatt
    15481 atacgtatgg gtgaagtatg tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg
    15541 agtcaaggtt atcgtcgttt ttttcctgaa ctagttatca gagagacaac gaattaagtc
    15601 ttattactat atatatattt atgaaattca tcgctatttt cttaaaataa tcagcgcatt
    15661 gctactgaaa acaatgaatc tgtgctatta ttgagaaaag ttccattttt gagaaggatg
    15721 gtttgaatga aagaaatcag acgaaaacat ttccgttttc ctgcgtacga tgatgagtta
    15781 ggcgtaaaat taaccaacga caagaagcga gtattatttg acggacaaga agatcttttg
    15841 actgaacaga atcagtcccc gtttgaacag atgaaagaat ttcatttcac gaacacgaac
    15901 accgagaaaa tccgtcgcaa gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag
    15961 gaaaatttgg aaaagcataa aggaaaatta ccggattata ccggccatta ccaatcagaa
    16021 atcacatcga caggacgaaa aaaacttttt ggtgattcct ctcgttctgt cagacaaagt
    16081 gaaacaagga cagagtccaa gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg
    16141 gagcgttctt attttgttcc taaatatgta cccgcatcga ttatcccaga ccctagagaa
    16201 agcaaaattt ctgaggatca tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg
    16261 ttgttcgata cagaaccgac gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga
    16321 acattcaatc gaacaaaaac gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa
    16381 aaaagccatg gtatcttaga acgctcatta caaggattga tcgatgatca aggaaactct
    16441 ttgggagaaa atagctattt taaataagtg atatacaaat ttatattcgg ctacagttgg
    16501 aaaggaaacg ctgcggcgtt tccttatttt atactctatc cgaaagttta tatcgtatcc
    16561 tcaaattttt ttgagtttaa gcgaattttt tctcccaata agtaagaaaa taatggcaaa
    16621 ataaaaaaac aaactatttt cgcagatcgt ttgcaaaaat agtttatttt tttataaaga
    16681 agattttact gaataatatc attttagttt acataatgat atttctgtaa attaaataaa
    16741 gtagtgaaaa ataagaatca gtgctctaac tgttcaagag gatcaagtgg ttcaaatgtt
    16801 tctgtagtct gttttgcttc caattggggc aaaacagatc caatttccag atacttcact
    16861 ttattcaatg tataagatag aatagaacag ctaagtaatg tatacagaaa atgtgtgaga
    16921 tccaaataaa gcaaggagac aactaaaata ccaaaagtaa tcaagaaaag acttttgcca
    16981 atcttaatat gaaaatattt ttcaatggct tgataaatga tatctgttcc gcctgtgctt
    17041 ccgccgaatc gaaagacgag accgcaacca atgccagaaa gaatacctgt catcaaacta
    17101 ttgatcagta gactatctac agcaaaatgt atatgaaggc tctcccataa atgtaaaaag
    17161 aaaactagcg caaatgttcc gacgatcgtc cggagaaaag cgtgttttcc aaaaagaaac
    17221 aatgagataa gcaataaagg taaattaaaa agcagtgaag tgacagaagg agcgaatcca
    17281 aaaagattat gcaaaagcaa agcaatacca gtaaccccac catctgccag cttagatgga
    17341 atattgattc ctgtcacagc taatccgtag attcctgcgc ctaaaatgat tccgcacaat
    17401 tctgtaaata aatttaattt tttcatttct agcctctttt cttttgaaaa ttttcagcaa
    17461 cttctttatt atactgactt atactcttac ttcaagagga aaatgaaaaa aaattatttt
    17521 tggctgtttc tttttattta tcaaataagg agtggaaaaa tgcgagcagc agacttattg
    17581 acaattctta caaaatcacc caaaaaaaca gagtatcaag gagttttttt ggctcaagaa
    17641 caaaaactgg tgcctgtgac aatggtccag ataaataaag caaatcaatt tatcttttta
    17701 tttgaacacg gcaaagcacc acttgctatg aaagacatct ttcttttttt gatgaagaac
    17761 cgtgagaaag ctgtttatta tcagtcaaaa gaagaactca ttcctcttta tggagtgaaa
    17821 gagatagaaa ataaagtggt gatataagta aaaataagaa aacaagacgt ttatgatgtc
    17881 aaaacaacca ttttttacat ttttggcaaa aaagtgtcat gatacagtat aatattcgtt
    17941 gaacaggcca ataaaggtgt tctcccccac gagatttcta ctttattggc tgtttctttt
    18001 tattaaaata aaagttgtgt gtgtaaaaaa gatatagctg attccaagtg acaatttcgt
    18061 atagattttt atggtattcg tagtgaaaaa acgagaacca aaaagttgat ccgactttgt
    18121 tgcttggatt ctttttttgt cagcatattt agaggtattt ttttcttttt tttgttcgtg
    18181 ctgtaaggtt aaggcaagac aagttagttg ggaaaaagga aagaaatgtt tatacttaaa
    18241 gaataacaga aaggaaggtc gcatgaataa ttttatacaa acattaagtg aaagaaaagg
    18301 cgagttgctg gaagctgcat ttcaacatct gtccatttct ctcatttctt tgctgattgc
    18361 agcactgatt gctattcctt tagcgatttg ggcagccaat cataaaaaaa tggcagaagt
    18421 cttattgcag atcacgggtg tgctccaaac gattccttca cttgccttat taggtttact
    18481 tatcccattt gtagggatcg gaactgttcc agcattgatt gcacttgtta tttacgcttt
    18541 gcttccgatt ttccaaaaca cgtatattgg tctagctgaa attgacccgt ctattgaaga
    18601 agcagcggta gcatttggta tgtccagaat gagacgcttg ctgaaagtag agcttcccat
    18661 cgcattacct gtcattatct caggtatccg aactgcctta gttttaatca tcggtacggc
    18721 tactttggca gcattgatcg gagcaggtgg attagggaca tttattttgt taggtattga
    18781 ccgaaataca cctgttttga cattgattgg tgcgatcagt tctgccttgc ttgcaattat
    18841 attcagcgga ctgattcgct ggttgcagca taagaaacct cgatattcca tcattacttt
    18901 agcagcagtg gtgtgtttat ttggcggcgt ttctttatat cagtctgcct tgtttaaaaa
    18961 tgaaaccatc acgattgctg gaaaattagg agcggaacct gaaatcctga tccaaatgta
    19021 taaagaattg attgaagaca acacaaaaac agaagttgaa ttaaaaccga attttggcaa
    19081 gacgagtttt ttgttcaatg cactggatca aggtcaaatc gatatctatc ctgagttcac
    19141 aggtactgtc ctagaaagct tggtgaaggt acctgaagca atgaaaagtg ctgatttgac
    19201 aaaagaaaaa acatatgacg aagcagaaga attattgaat aagcaattcg atatgacatt
    19261 attaaaacca atggcttacc aaaatacgta tgccttggca gtcaagagat cttttgcgaa
    19321 cgaacatcaa cttaaaaaga tttccgattt ggcagctatc tctgatcaga tcaaagcggg
    19381 attcacactg gaatttattg atcgttcaga tggatataaa ggaatccaag aagcatatgg
    19441 cttgaatttt tcaagtgtac aaagcatgga gccaagtttg cgttaccaag caatcaataa
    19501 tggagatgtc aatattgtgg atgcctattc cacagatagc cagctcaagg agtacgatct
    19561 agtgactcta gaagatgatc gagggttatt cccagcttat caaggcgcac cgctgatgaa
    19621 aaaagatttt gctaaagaac atcctgaaat cgttaaagca ctgaataaat taagcggaaa
    19681 aataacagag gatcaaatga ttgatatgaa ttaccaagtc aatgtcgaaa agaaaaagcc
    19741 gacagaagta gcacgaatct tcctgaaaaa tgaaggatta gtaaaggagg aagatcagtg
    19801 actaaattaa tcgagttcaa acatgtgcaa aaaaagtatg atggaaagta tgtcatcgat
    19861 gacctaaatc tggcaatcaa aaaaggagaa atctttgttt tggttggtcc ttccggtagt
    19921 ggaaaaacaa ctactttgaa aatgatcaat ggattatcta aaccatcagc aggagacatc
    19981 tacttcaaag gaaaatcgtt aaatgaatat aatctgcaga aaatgcggtg gaatatggga
    20041 tatgtgctgc agcaaatcgc tttgttcccg accatgacag tcaagcaaaa tattgaagtc
    20101 atccctgaaa tgttaggttg ggaaaagcag aaaagagcag accgtgtaga tgaacttctt
    20161 caaaaagttg gcttatctcc tgatatttat cgagatcgta tgcctagaga gttgtctgga
    20221 ggagaacagc aacggatcgg gatcatccga gctattgctg caagcccaga tgtgattttg
    20281 atggacgagc catttagcgc attagatccg atttcaagaa actcattaca agaattggtt
    20341 ttgtccttac acgaagaatt gggcacgacc atcgttttcg ttacgcacaa tatggaagaa
    20401 gcaatcaagc taggtgatcg tattgcattt atgaaggatg gagaaattat ccaatgtgac
    20461 acaccagagc agttattgat gaatcctaaa aacgactacg ttcggcattt ctttgatgaa
    20521 ccgaaacaga cgaaagagtg gcgagtagaa gatttagtcg tcaatggata tttcttaaat
    20581 gaaataccag aaaatgctcg tcaacaagtt tgctttgata cgcctatgaa agaagtatat
    20641 tcgcttcttt cggtgtatcc aagcattgtg atagtagaaa agcaaagaag tattgggtcg
    20701 ctgacaagta aagaaatctt tgctttttta agtcgagagg aggaaggaaa tgaaaacata
    20761 tgatgcaatc atcatcggtt caggtgtgag cggcttaagt gctgcttatg gattgaaaga
    20821 agcaggtaaa acagtactag tggtcgaaga agatctatgg ggcgggactt gtcctaaccg
    20881 tggatgtgat cctaaaaaag tcttgttaag tgcagtagag gcacgtaatc gggtaaaaca
    20941 attgagtgga aaaggtttta acgagatccc aactgccaac tgggaagaac ttcaaaaatt
    21001 caaacgaaca tttacggatc cagtcccaga aagccgaaaa aaacaattag cagaagctga
    21061 aatcgatcat ctttcaggaa ccgctcgttt tttggatgat tcatctattg aagtcaacga
    21121 agaagtcttc catgcggatt acttagtttt ggcaaccggg caaagaccta cgatcttacc
    21181 agtggaggga aaggaatact tgaaaactag tgcagacttt ttatcattgc ctgtactgcc
    21241 taaagaaatc atttttatcg gtggtggtta tattgctttt gagctagcta cgattgccaa
    21301 tgctgcaggg agcaaagtaa ccattgtcca tcataatcaa cggccattaa aagaatttga
    21361 ggcgtctttg gtagaggaag ccgtccatca aatggaagca tctggcattc agtttgcttt
    21421 tggtgttgaa acccaaaaga tcatttcaga aggtactcgc tatcgtttag tcggaaaaga
    21481 gacggaatta gtagcagata tgattttttg tgcgactggg agacagccga atacagaatc
    21541 tttagcactt gagcaagcga atatcgtctt tgataaacac ggaattgcag tcaatgatta
    21601 tctgcaaacg agcaatccaa aaatttttgc gtgtggagat attgtttcaa ggaaaacccc
    21661 aaaattgaca ccagtcgcca cttttgaagg aaattatgta gctaagcgga taacggatgc
    21721 aacaagcgaa cctatcaaat atccgattat acctacgatc gtctacgcca gtcctaaact
    21781 tgctgaagta ggagttacaa aaagtcacgc ctcttcgtca gatcaagtgg tagagatgga
    21841 tcttaccagt tggttcacct atcatcgtgt caatgaacca gttgctaagg ctgaattgac
    21901 ctttgaccag caaaattatt tgattggtgc tgcagtgatt agcgagcaag cagacgaatt
    21961 gatcgatgat ttgacactcg tcatcaatca aaaattgact aaaaaagagt tggatagcta
    22021 tatcatgggg tatccgacat tggcaagtga tctctcctat ttattgaaat aatcagcatg
    22081 ataactaaag cgcttgttgc tttttccttc ttgtgctaca ctttaaaaaa tgaatacctg
    22141 tttctgggga tgtcgtagac gacatgagag taagttgtga acttagaccc attgaacctg
    22201 tcagttaata ctggtgaagg gagaaacata gcagacgctt gaagcgggtt actatgtcgt
    22261 gcccatttca agcgtttttt gttattcata ttagaagaaa ggaggtaaca aaatgaagaa
    22321 aaatctttat ccagcagtca gtatggcatt cattttaatt atttggcaga ttgttgtcat
    22381 agtttggaaa atacctgcct ttattttacc cggacctgct gctgtatttt ctgcattagc
    22441 ggcagatgca caagtattac tttcccattc tgtcacgaca ttaaatgaag cagtgcttgg
    22501 tttggtaata gctagcgttc tttcttttat tactgccttg gcaatggatt attggaagtt
    22561 tttagaaatg tctttttatc cgttactagt gatttcacaa atgttgccaa tcatggtgtt
    22621 agggccatta cttaccctat ggtttggttt cggtatgctt ccaaaagtga tccttgttgt
    22681 cttgataagc tattttccga tcgtagtttc tttttcggat acattgagga aagtatcaaa
    22741 agagcaaatc atctttttga aaacgatggg ggcagataca gggaagattt atcgaatata
    22801 taaaatacca gcgggtttat ccggcttctt ttccggttta aaagtggctg ctacttattg
    22861 tgtcagcgga gcagtcgttg gagaatggct aagcgctcaa gcaggcttag ggtattacat
    22921 gatacgcgta aaaaacagct accagatcga taaagttttt gctgcgatca tttgtgtcat
    22981 tctattgagt ttattgctga atggcgcttg ttcattattg aaaaaagggt attatcgtat
    23041 gctttatatt ttttaagaga aggggatcaa atgaaaaaat tatggctttt attaccgcta
    23101 ctgttgcttt tatccgcttg tggcactgca aaagaaacaa gtagcaaaca agaaataaag
    23161 gatttgaaaa aagtgacact tgttttggat tatgtaccga atactaatca tactggcatc
    23221 tacctagcaa aagaaaaagg ctattataaa gaggctggtt taaatgtcca aatcatcgaa
    23281 ccaggagata attcgacaag tattggtcta gtaggtgcgg acaaagcaca atttggtgta
    23341 agttatcaag aagatgtcac ttatgctcac gcagatggac agaatatacc agtcaaagca
    23401 attgctacag tgatcaaaca taatacttct ggattcgcca ccctatcaga cagtaacatc
    23461 cattcaccaa aagattttga aggaaaaaca tatgcaggtt ggcaaagtcc aagtgaagaa
    23521 gctgtcttaa aagctgtgat ggaaaaagac ggcggggact tttcaaaact gacaatggta
    23581 ggaagcaatg gagaagggcc agaaagctta ggaaaatcct cagatatcca atggtacttt
    23641 gaaggttggg atatgatcaa agcaaaagaa gcgggtattg aactaaacta tatcccattg
    23701 aaagaattag atgaacggtt agattactat actccggtaa ttattacgaa tgatcaactg
    23761 atcaaaagtg atccagaatt ggtacagtct tttatggatg ccacgaaaaa aggctatcag
    23821 gaagctatta aggatccaaa tgacagtgct aaactgttgc aaaaatatgc aaaagaaaat
    23881 gatcggacgt ttttagaaga atcacaagca ttcttgtcaa aaaattatac ggatgatcct
    23941 gagaattggg gattgatgga agaaaaagtt tggcgcaatt atacagcttt tatgcaggaa
    24001 aacggattga tcaagcaaga tgtaccttcg caagaactat tcactaatca gtttataaaa
    24061 taggagtgat agatatgtca atccaaatca gccacttgaa tgtagtcatt gaaaacctga
    24121 aaatattaga agacatccat ttcactatcc ctaaagatac ctttacatcg attgtcgcgc
    24181 caagcggagc cggtaagtca acattgctga aaactttaac aggagtccag cctatgactt
    24241 ctggaacgat caaagtagac gatcaaaaag taactggtct ccatacagca tttagttata
    24301 tgccgcaaga agatatgtta cttccttggc tgagcgtcta tcaaaacgtt acgttatatc
    24361 aaaaaatcaa tcatctaaca atcaatgaag aacaagtaag ggattatctg gatatctttg
    24421 ggttaaacgg ctatgaacat tttttgccgg aacaattatc tggcgggatg aagcagcgaa
    24481 cggccttgtt acgaacaatc atgaatcctt cttcttactt gcttcttgat gaaccatttg
    24541 gagcgttaga tgctatgacc agagggcaga tgcaagactg gatgttgaag ctgcctaaaa
    24601 aagcaaagcg gactacttta cttgtgacgc atgatatcga agaagcaatt tatttatcag
    24661 acaggattct tgtgctttcc gcccgtccgg cacatgtgat tgctgagatt caagtgcctg
    24721 aaaaacgacg gtcacgtgaa tggctactgc agcaatcaga gttgaagcaa acgatttatc
    24781 agttattggc aggtgaatta gatgttaagt gatgctcatt gtcatttaga tgggagtcag
    24841 agtttagcac tccttcagca ggaacacacg attttgacga tcatcaattg tgattcgcca
    24901 gaagaatgga aggaaaacag acagcttgca gcaagtaaga ctcaagcctt aagttacggt
    24961 atccatcctt ggaaagcaga tagctatacg tttgaacaag ttgagccttt tttgaaaaaa
    25021 gcaaggatca tcggtgaaat agggttagat aatatttgga cgaatgtacc gatgactaca
    25081 caaaaaaaag tatttgagcg acagcttgct tttgcagcca taaatgagaa acctgttgta
    25141 ctgcacacaa aaggatgcga aaaagagatc ttaaattata tccaagctta ccctaatcac
    25201 tatttgatcc attggtattc ttctttggac tatcaacaag actatattga tttaggctgt
    25261 tatttcagca ttggtcttga tttgaaaaag aatccagcag tctggcaact agctaaagca
    25321 gtcccaatcg accacttact tattgaaacg gatggactac atgcagcaga gtgggcttta
    25381 ggggggattt tttcagaaaa agattatgtc tcactgcttg aagaacagtt aaagacaatc
    25441 gctgagatca aagagctatc agttgaagaa gttaaaaaaa atacttatca aaacttggaa
    25501 cgtttcatta gattaggtaa ataaaaaaga gatgtgacac aactagtgtc gcacctcttt
    25561 ttggctttct ttgtataaaa gtaaactgtt tgattaataa atgaatctta gtaccaaccg
    25621 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
    25681 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
    25741 tggtaacgtc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
    25801 gcgatccatt cttttgcaga gcttgtgctt gctggagtag cagcagtttc tgttacagca
    25861 ggagcaggtt gtgtttcagc aacaggcgct gcttgttttt ccgtttgaac cgtttcttgt
    25921 acaacaggtt gttcagcttg gactggtgct tcttgaacgg ctgcttgagg ttcagcagct
    25981 gcttgtgcag agccttctgt tgggattgtt aattgttgtc ctgagtaaat caggttaata
    26041 tcagaaattg aatttgattc tgcaatggca ttgatcaagg agttatcacc aacgtatttt
    26101 tgagaaatcg ttgatagagt atcacctgat tgaacggtat aagcttcgtc cgcatgagca
    26161 gtcgttccca tgaagaatgc agcgccggca gccaaagttg ttccaaaaag taaagtttta
    26221 agtgatgtca tgaaaaatct cctttattcg ttaaaaagtt ttatttgttg tgttcaacga
    26281 ggaatacttt aacacgtgaa aatatttcat aggtaaaaga agcatgacaa tttatgacaa
    26341 atccgcgttt tttgtaataa atacagtaat ctatgaaatg aaaagggtta cgtttaataa
    26401 aacttgcttt tttgtttttg ttcaccataa atatttattt aagaaagtat taacctgcat
    26461 ttttctctat aaaaaagatt ttttaaatag ataaaaaagg aggattaaat gagtatttga
    26521 tagtatttat attaaaatta catatgtaat ttaaaaacgc ttgacattgt gtttgagatc
    26581 acttactttt agcatgtaaa agtattggat taaggagcta tgcaaatgaa aaagaaattt
    26641 attactggtg ggattgtcgt tgcatttcta tgtctaattg gctacacagg cacgcggtac
    26701 gcaaatgaaa ttattttgtg gggtggagag acagatattg atacaatcaa tgagaatctg
    26761 acacagcttg atcatacatt gacagataaa gagcaagcga ttacagatct taccgctcga
    26821 ctagcacaaa aagaacagac attgcaagat tcaaaggcag atgtccaaga ataccaaaca
    26881 aaagtaaatg aattagaaaa ccaaaaaaat caatcggcag cagaaaaaca aaacttagaa
    26941 agtcaattag tcagcaagga tgcacaacta caaagcaaac aaaatgaaat caatgtcaaa
    27001 ctagatgaga tcaaccaaaa aaatgatgaa atcaatcatc tgaatgcaga ttggacagca
    27061 agactacaag aagcacaagc aaaactttca gaattacaaa atactgtaaa tggtttgaat
    27121 caacaaatcg caacactgac acaagaaaaa acaaatgtag aaatcagtta acagatacac
    27181 aaaagaaatt agacgaagca actgctgaaa actcaagctt gaaacaatat attcaaaagt
    27241 tagaaaaagc aaaagatgaa gtggaagata cagctgataa atctcagcaa attgttgaag
    27301 aacatttgcg taaataatct ttcttaacaa gtaaacctaa attagtaaag aagcaacagt
    27361 aatctatact gctgcctctt ttttttagta catgtccttc atcttcgcag gaagtgccaa
    27421 tatgatttta agaaaggtaa tagacaaaaa gtcaaaaaga taaaacagag tttcttcttt
    27481 acaaagggaa tgtatgttcg tataatggta ttgaggtgaa ggttatggag aaaaaatatg
    27541 gaacagtaga taagatccgt actttgcaag ttagtgccag accgttgatc cgtttcagtt
    27601 taaatggggt caattgtttg attgcttccc atagtttgaa cttcctagct gaagtagacg
    27661 aagggatgaa gttagtcgtt acaggttatt ataataatag aaagcaattt gttgtgagat
    27721 cctatcatgt gttagggaaa cccaaaattg tggtagaata tgagaaaagt ctatacccaa
    27781 gaaaaaaggt ccgacaagat gaaagaataa actagaaaac aagcggctga gacaaaaatg
    27841 tcctagccgc ttgttttgga aagatggatg cagacgatgg gatttgaacc cacacgagca
    27901 tacgctcaca agaacctcaa tctatatata actacatgta tttaagtatt tgtgatgaat
    27961 cactaaactt tccaaactca tttgagggat attgcttaaa gattgaacag atcaattcga
    28021 agccctatta gtattcgctc aatgggggga tcagaggcca taaaaagaga ctagtcatta
    28081 ctagtctcag gtagtggaat tgtaggattt taaacgttcc tggagagtac cggtatgtaa
    28141 ctcgaagagg tgattatcat agtcgtaaaa gtaaattgaa gaagcctcgc ctttcacacg
    28201 agaacgtcct ggttcgacag ttaaacctaa catttggatt tttgagacaa acgaatctat
    28261 atcttgttcg tcaattttaa aagcaatatg attgtatgtc tttggcagtt tattcgacga
    28321 attctgcatt actgcaatcc ataggtcctt aactaagaaa aatttttctg gatataatga
    28381 aaattttttt gttgactatc gtagatttct tttgcatcaa ataaggtatt taacaattaa
    28441 gtatttaaaa gataactttt aagttatctt taagtaatgc ttaaagctat gttatactct
    28501 attaggaaat ataagaagga gtataaaaat gaacaaacat aatagaaaaa taacgaataa
    28561 caaagaactg atcttgttac taatagggat ttggggtatc tttttactct ttaatatgta
    28621 tatgccgact atgagagcgg atgacgtagt ttatgcaagt agattagatg aacttggcta
    28681 tttgggtgct tcgatagaac attataagac atggagttca agaattatta tagaactatt
    28741 cttaatgttc ttttctaaac actttatgct atggaaatta ttaaattcta cgattatgtt
    28801 aggaattgta gtattacttt gcaagtat
//
LOCUS       DAFEPQ010000031        28137 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.031, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000031
VERSION     DAFEPQ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.031"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            207..2741
                     /gene="secA"
                     /locus_tag="LP543_001479"
     CDS             207..2741
                     /gene="secA"
                     /locus_tag="LP543_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBL2321649.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2867..3968
                     /gene="prfB"
                     /locus_tag="LP543_001480"
     CDS             join(2867..2938,2940..3968)
                     /gene="prfB"
                     /locus_tag="LP543_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBL2321650.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4073..4759
                     /gene="ftsE"
                     /locus_tag="LP543_001481"
     CDS             4073..4759
                     /gene="ftsE"
                     /locus_tag="LP543_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBL2321651.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4743..5636
                     /locus_tag="LP543_001482"
     CDS             4743..5636
                     /locus_tag="LP543_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2321652.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            5788..7236
                     /gene="cls"
                     /locus_tag="LP543_001483"
     CDS             5788..7236
                     /gene="cls"
                     /locus_tag="LP543_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBL2321653.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGGKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7573..8430
                     /locus_tag="LP543_001484"
     CDS             7573..8430
                     /locus_tag="LP543_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBL2321654.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8545..9465
                     /gene="pstC"
                     /locus_tag="LP543_001485"
     CDS             8545..9465
                     /gene="pstC"
                     /locus_tag="LP543_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBL2321655.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9465..10349
                     /gene="pstA"
                     /locus_tag="LP543_001486"
     CDS             9465..10349
                     /gene="pstA"
                     /locus_tag="LP543_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBL2321656.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10364..11170
                     /gene="pstB"
                     /locus_tag="LP543_001487"
     CDS             10364..11170
                     /gene="pstB"
                     /locus_tag="LP543_001487"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBL2321657.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11186..11944
                     /locus_tag="LP543_001488"
     CDS             11186..11944
                     /locus_tag="LP543_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBL2321658.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11957..12634
                     /gene="phoU"
                     /locus_tag="LP543_001489"
     CDS             11957..12634
                     /gene="phoU"
                     /locus_tag="LP543_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBL2321659.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            12872..13903
                     /locus_tag="LP543_001490"
     CDS             12872..13903
                     /locus_tag="LP543_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBL2321660.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSFLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            13900..14667
                     /locus_tag="LP543_001491"
     CDS             13900..14667
                     /locus_tag="LP543_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBL2321661.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14767..16335
                     /gene="liaX"
                     /locus_tag="LP543_001492"
     CDS             14767..16335
                     /gene="liaX"
                     /locus_tag="LP543_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBL2321662.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16632..16952
                     /locus_tag="LP543_001493"
     CDS             16632..16952
                     /locus_tag="LP543_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBL2321663.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            16952..17302
                     /locus_tag="LP543_001494"
     CDS             16952..17302
                     /locus_tag="LP543_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBL2321664.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17469..18407
                     /locus_tag="LP543_001495"
     CDS             17469..18407
                     /locus_tag="LP543_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBL2321665.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18422..19252
                     /locus_tag="LP543_001496"
     CDS             18422..19252
                     /locus_tag="LP543_001496"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBL2321666.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19296..20321
                     /locus_tag="LP543_001497"
     CDS             19296..20321
                     /locus_tag="LP543_001497"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBL2321667.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHHASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20336..21274
                     /gene="galU"
                     /locus_tag="LP543_001498"
     CDS             20336..21274
                     /gene="galU"
                     /locus_tag="LP543_001498"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBL2321668.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            21399..21839
                     /locus_tag="LP543_001499"
     CDS             21399..21839
                     /locus_tag="LP543_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBL2321669.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21842..22444
                     /locus_tag="LP543_001500"
     CDS             21842..22444
                     /locus_tag="LP543_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBL2321670.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(22507..23610)
                     /locus_tag="LP543_001501"
     CDS             complement(22507..23610)
                     /locus_tag="LP543_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBL2321671.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            23820..24821
                     /gene="ccpA"
                     /locus_tag="LP543_001502"
     CDS             23820..24821
                     /gene="ccpA"
                     /locus_tag="LP543_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBL2321672.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            25176..25571
                     /locus_tag="LP543_001503"
     CDS             25176..25571
                     /locus_tag="LP543_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321673.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            25571..25822
                     /locus_tag="LP543_001504"
     CDS             25571..25822
                     /locus_tag="LP543_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321674.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            25865..26260
                     /locus_tag="LP543_001505"
     CDS             25865..26260
                     /locus_tag="LP543_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321675.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            26257..27513
                     /locus_tag="LP543_001506"
     CDS             26257..27513
                     /locus_tag="LP543_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HBL2321676.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGQLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            27525..27848
                     /locus_tag="LP543_001507"
     CDS             27525..27848
                     /locus_tag="LP543_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321677.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            27841..28107
                     /locus_tag="LP543_001508"
     CDS             27841..28107
                     /locus_tag="LP543_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321678.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         9460 a         4833 c         6083 g         7761 t
ORIGIN      
        1 taacaacgaa aacttgcagc agctactgac agctgccgca agtttttttg agtttcctcc
       61 tcttttagat gaaatatgaa taaattatga agttcttact ttaatacgct taagatattt
      121 caattatcta taactaatga taaaatgaaa agtaaagtct acatagtctg gataatacac
      181 agaaggaaaa ggagattaca caacgcatgg ccaatttgat tcgttcactt atcgaaaacg
      241 ataagaaaga actaaaacgt ttagatagca ttgcaaaaaa agttgaagca tacgctgacc
      301 aaatggcagc attaactgat gaacaattac aggcaaaaac tgctgaattc aagggcagat
      361 accaaaaagg agaaacatta gatcaattac ttccggaagc ttttgcagtc gtccgagaag
      421 cagcaaaacg tgtattgggg ttatatccac accacgtaca attgatgggt ggaatcgtgc
      481 ttcacgatgg aaatatccct gaaatgcgta ctggtgaagg taaaaccttg accgcaacga
      541 tgccagtata tctaaatgct ctgactggcg aaggggtaca cgtagttacc gtcaatgaat
      601 atttagcaac ccgtgactcg acggaaatgg gcgaacttta taatttctta ggattaacag
      661 tggggttgaa tatcaactca aaatcttcgg atgaaaaacg cgaggcttat aattgtgata
      721 tcacttatag tacgaataat gaattaggat ttgactactt gagagacaat atggtcgttt
      781 accgccatca aatggtacaa cgtccgctga attatgccat tgtcgatgaa gttgactcaa
      841 tcttgattga tgaagcgaga acgccgctga tcatttcagg gcaagcagaa aaatccacag
      901 ctttatatac aagagcagac aacttcgtga aacgtttgaa agaagaagaa gattataaaa
      961 tcgatgtaca atcaaaaaca atcagtttaa ccgaagcagg gatcgaaaaa gcagaagaaa
     1021 cattcggttt ggataactta tacgacattg aaaatactgc attgacccat cacttggatc
     1081 aagcattgag agccaactat atcatgcttt tagatatcga ttatgttgtc caagacggaa
     1141 aagtactgat tgttgaccag ttcactggtc ggatcatgga tggacgccgt tattcagatg
     1201 gacttcacca agcaatcgaa gctaaagaag gcgtagagat cgaagacgaa acgaaaacca
     1261 tggcgacgat cactttccag aactatttcc gtatgtacaa gaaacttgcg ggaatgactg
     1321 gtacagctaa aacagaagaa gaagaattca gagaaattta taacatccaa gtataccaga
     1381 ttccgacaaa tcgtccggtt atccgtgatg atcgtccaga tttgctttac ccaacattac
     1441 aaagcaagtt ccacgctgtc gtgcaagata tcaaagatcg ctaccgcaaa ggtcaacctg
     1501 tgttagtcgg aactgttgcg gttgaaacat ctgagttatt atcagatatg ctgaatcaag
     1561 aacgtattcc tcatgaagtg ctgaatgcga aaaatcactt taaagaagca gagatcatca
     1621 tgaatgctgg tcaaaaaggt gctgtgacga ttgctaccaa catggctggt cggggaacag
     1681 atatcaagtt aggacttggg gtaagagaac ttggtggtct agcagttatc gggacagaac
     1741 gacatgaatc tcgtcgtatc gataaccagc ttcgaggacg tgcaggacgt caaggagacc
     1801 ctggtatgtc acagttctac ttgtcattgg aagatgattt gatgaaacga ttcggttcag
     1861 aacgaatcaa atctttctta gatcggatga agatcggtga ggaagatgct gttatccaaa
     1921 gcaagatgct gacgaaacaa gtagaatctg cacaaaaacg tgtagaagga aataactatg
     1981 acacacgtaa aaacgtctta caatacgatg atgtgatgcg tgaacaacgt gaagtaatct
     2041 ataaacagcg acaagaagta atcatggaag aaaaaagctt atccaagcca ctaatgaaca
     2101 tggtcaaacg gacaatcagt cgtgtagtag atgcgcatac acaattagag aaagaaaatt
     2161 ggaatttgga aagtatcatc gattttgccg gcagtactct tgtccatgaa gatagtatta
     2221 ctcttgcaga tatcgaagga aaaacagctc aggagatcaa agagtatcta aacgaacgag
     2281 cacaagaagt atttaatacc aaagcggctc aactaaatgg tccagagcaa ctgttggaat
     2341 ttgaaaaagt cgtgatcttg cgtgttgtgg actctaaatg gacagaccat attgatgcaa
     2401 tggatcaatt acgtcagtca atcggcttac gtgcttatgg acagaacaat ccattagtcg
     2461 aatatcaaac agaaggctat aagatgtttg aagatatggt aggcgccatc gagtatgaag
     2521 tcactcgaat ctttatgaaa gctgagatcc gtcaaaacgt acagcgtgaa caggtttctc
     2581 aaggaacagc ttctcactca gaagacggag atgccaaaga aaatagtaat actagcgcga
     2641 aaaaacagcc aatccatgtg aacaaggtcg gaagaaatga tccttgtcct tgtggaagcg
     2701 ggaaaaaata taagaactgt catggaaaaa atgcaaacta atttctgaca gcaggattgg
     2761 tgagacaaat tcttttttta agaattcgtc tcaccttctt tctgttattt gctgattctg
     2821 ttagactaga tataatgaaa aaagtatagt gaaagaagga aaaaacatgg aaatatccga
     2881 aattcgaaat ctcctgacag aaatgcagga aaaggtcaca agcttcagga ggtctctttg
     2941 acttagacca gctggaagaa gacattgcgg aagcagaagc aaaaatggct gaacctggtt
     3001 tttgggatga tagcgaagct gctcaaaaag tgatcagtga aaacaatgca aataaagaaa
     3061 cttatgatca attccattct cttgctgaag aactggaaga aatcgaattg atggtggaga
     3121 tgttgcagga agaacctgat gcagccatgc aagaagaagc agaaacacgt atcaatgcgc
     3181 tagatgaaaa aatgaagaca tatgaattat ctatgttgtt agatggtcct tatgatcgaa
     3241 acaacgccat cgttgaattg catccagggg ctggcggaac ggagtcccaa gattggggca
     3301 gtatgctttt acgtatgtat acacgctggg cagagcagca tgggtatcaa gtagagacac
     3361 tagattatca agcaggtgat gaagcaggaa tcaagagtgt gaccctactg atcaaaggac
     3421 acaacgcatt tggttatctg aaatcagaaa aaggtgttca tcgccttgta cgtatctcgc
     3481 cttttgactc agcgaaacga cgtcatactt ctttttgttc ggtagatgtg atgccagagc
     3541 ttgatgaaac gatcgatata gaaatcaatc cagatgattt gaaaatcgat acgtaccgag
     3601 caagtggtgc aggtggtcag cacatcaata aaacagaatc tgctgtacgg atcactcact
     3661 tgcctacagg aacagtagta gctagtcagg cacaacgttc acaattgaaa aaccgtgagc
     3721 aagcgatggg gatgttaaaa gctaagcttt atcaattaga agtggaaaag aaagaacaag
     3781 aagcagctgc acttcgagga gaacaaaaag aaatcggctg gggttcacag atccgttctt
     3841 atgttttcca tccatattct atggtaaaag accatcggac aaacgtagag acaggaaatg
     3901 tccaagcagt tatggacggt gaccttgatt tatttattga cggctattta aaaatgcatc
     3961 tacaataaga acccattgtt aaattgaaat aaaattgtaa gattttgcaa gcatgttgaa
     4021 acattgccat gctataatag ctaaagactg aaaaagatat ggagagaacg ccatgattga
     4081 aatgaaggat gtaatgaaga agtactcaaa tggtacgacc gctatccgca atctctcaat
     4141 tgtcatcgaa caaggagagt ttgtctatgt ggttggtccg tctggagccg gtaaatctac
     4201 attcatcaaa ttgatgtatc gagaagaaaa agcttcaaaa ggaactttga acgtcgcagg
     4261 tcatgatcta atagcaatca aaaaccgcga agttccttat ctacgcagag aaatcggcgt
     4321 ggtgttccaa gactataaat tattaccaag aaaaacagta tatgaaaatg ttgcgtacgc
     4381 tatgcaagtt atcggcagaa aacctcggga tatcaaaaaa cgagtaatgg aagtgctgga
     4441 ccttgttggt ttgaaacaca aagttcgagt ctttcctagc gaattatccg gtggggaaca
     4501 gcaacgtgtc tctattgctc gggcaatagt gaatacgcct aaagtactta tcgcggatga
     4561 accgacagga aatctggatc cagaaaactc atgggaaatc atgaaattat tagatcggat
     4621 caatgcccaa ggaactactg tcgtgatggc tacacataac agtacgatcg taaatacgat
     4681 tcggcaccga gtaattgcta tcgaaaacgg ccgcattatt cgagatcaag cggaagggga
     4741 atatggatac gatgattaga acctttttcc gccatttatt agaaagtatc aagagtttga
     4801 aacgtaacgg ctggatgaca gttgcttcag tcagtgcggt aacgatcaca ttgactttag
     4861 tcggtgtatt catggcagtt atcatgaacg caactaaatt agctcaagat atagaaggaa
     4921 acgttgatgt ctctgtattc gtagatatcg gtacaaaaga cgaagagatg aaaaagctag
     4981 aaaaagaatt gaatgactta gatcatgtta aaaaagttga attttcaagc aaagaacagg
     5041 aattgaaaaa aatccaaaac gaaatgggtg attcctggaa attgtttgaa ggtgacaata
     5101 acccgttata tgatgtgtat gtagtaagtg ctactgatcc ttcttataca aaacaagtag
     5161 cagaagaagc agcagatttg aagaatgttt ctcgttctga ttacggtgga gcttcctcag
     5221 atcggatttt ccaaatcgct ggtgcagttc ggacatgggg attagttgca gcggcattgc
     5281 tgctgttcgt agcgatgttt ctcatttcga acacgattcg gatcacgatt ttgtctagac
     5341 agcgtgaaat tcaaatcatg cgtttagtcg gtgcaaaaaa tggatatatt cgctggccgt
     5401 ttttccttga aggtggctgg atcggtctga tcggtgcgat tcttccgatt ttgatcatca
     5461 catttggtta tgcatggttt ttccaattga tcaatccatc attattacgt tcccattatt
     5521 cattgattca tccacaaaat atcgtttgga aaatcaatct tttaatggtc ggcattggtg
     5581 tgatcattgg ttcacttggc tcgatcattt ctatgagacg tttcttgaaa ttctaatacc
     5641 acaatataaa taaaatgaga aagttggaca gtttcgttcc agctttcttt ttttattggc
     5701 taggagaata gaaggaagcg gcttttttcc ctttacttta aaaaacaact tgggtattct
     5761 taagttggta gaagaagagg tgaagccgtg aaaattatcg ttgacaattt ttttacgatc
     5821 cttttgatta tgaatatttt gctttccttt atcatcgttt ttcgagaaag aaaggaaact
     5881 gctcaaacat gggcatgggt gctcgttttg atgttcattc cagttgtagg atttatttta
     5941 tatatttttt taggaagagg gatttccaaa gagaaaatct ttgatttgaa gatccaaggg
     6001 aaaatcggta agaacctgga aattgaagaa gacaaacagg cattgatgcg agggctttat
     6061 cctcatccgc ctactggaca agtagatgtg aaacaattga tctatatgct cacggtattt
     6121 gaaagtacgc tctatacgac taaaaatgaa atcactttat ttacagatgg tcgtgaaaaa
     6181 ttcgatgcgc tgattgaaga tatcaagcag gcagaagacc atatccactt ccagtattat
     6241 atttatcgca gtgatgcatt aggtgaagaa gttcgagatg cattgacaga ggcggcaaga
     6301 cgagggggaa aagtcagagt ccttcttgat gcatggggtt ccacacaagt ttcttccagc
     6361 ttttttcaga atttgaaaaa agcaggcgga gaagtcgcat ttttctttcc tctatttgtt
     6421 ccgtatatta atcctcggat caattatcgc aatcatcgta aaatcgtagt tattgatggg
     6481 aaaatcggat atacaggtgg gttcaatgtt ggaaatgaat atctaggatt ggtaaagaaa
     6541 tttggttatt ggcgagacaa tcatttgcgt atttacggag aagcggtgta taccttgcaa
     6601 aaccgttttt tgatggactg gaattctcag catacaaaag aagtaggcta ttctcccaaa
     6661 ttttttccgt ccattcactc tactggtgat atagctgttc aaatcgtgac aagcggacca
     6721 gataccgaac atgaacaaat caaaatgact tatttgaaaa tgatttcgtt agccaaacgg
     6781 gagatattga tccaaacgcc ttattacata ccggatggat cgattcatga agcactgaag
     6841 ttggctcttc tttcaggcgt aaaagttcat atccaaatac ctaacaaacc agatcatctt
     6901 ctcgtttact gggcaacgta ttctttcgct gcagagctaa tcgagtatgg agcaaggatc
     6961 gaaacatatg agaatggatt tattcatgca aagacgatga ttatcgatgg tggcattgtt
     7021 tcagttggct cagcaaatat cgatgttcgt tcatttcgct tggactttga ggtcaataca
     7081 ctggtttatg atgaacgaat ggcttcacgt gtaagaaaag ctttttttga agactcgaaa
     7141 atttctacgc atctcacaaa agaaatgtat gaaaaccgag gaataatcat caagatgaaa
     7201 gaaggattag caagattgat ttcgccgctc ctttaaaaca taaaaaggac atgagaacca
     7261 tcaacagatg gagatctcgt gtccttttct aacggggcca tttctgagag tacgtcagaa
     7321 atggagaaag cgtctcgcat gagcgaaatt aactttgggg tatctcttgt tttttgggga
     7381 aagcaagaaa taagctaata tatgctatta aaagagacac atttcaaagc cagtatagca
     7441 tagaaacttt taatgagata gtttttttta caattatgag aatttatttt gttatttaat
     7501 gattttgatt cgttttcatc aagtaatgaa aatgataaaa taaacatact ttaggattaa
     7561 aggagatttc taatgaaaaa acttaaatta ttcttaccaa taataggatt agctattctt
     7621 ttatcaggct gttcaaagtg gattgatcga ggagaatcca ttacggctgt tggctcttct
     7681 gctttacagc ctttagtaga aacagttgct gaaacttacc agacagaaaa tcctggaaaa
     7741 ttcatcaatg tacaaggggg cggaagcgga actggtttgt ctcaagtaca gtcaggagca
     7801 gtcgacatcg gaaactctga tttgtttgcg gaagaaaaat cggggatcga tgcctcagca
     7861 ttagtcgatc atcgagtagc agttgttggg atcactccta ttgtgaacaa ggatattggc
     7921 gtaaaagaca tcagtatgga aaacctgaaa aaactattta caggcgagat caaaaactgg
     7981 agtgaagtcg gtggaaaaga tcagccagta gttatattga accgcgcttc aggaagcgga
     8041 acccgtagca cgtttgaaaa atgggtattg gacggagaaa cagctgttca ggcacaagaa
     8101 caagattcaa gcggaatggt gcgccagatc gttgccgaca cacccggtgc aatcagctat
     8161 gttgcttttt cttatgtttc cgatgatgta gatacgttga atgttaacgg tgtagaaccg
     8221 acagatgaaa atgtgacaac gaacaaatgg acgatttggg catatgagca tatgtacaca
     8281 agagatcatc caaaaaaact gacaaaagaa tttttagact atatcttatc tgatgaagta
     8341 caagataaca tcgttggaga actaggttat atccctgttt ctcatatgaa agtcgaacgt
     8401 gattggcaag gaaatatcgt taaagagtaa tctttacaca aaatttacaa acttagctaa
     8461 acttttacat gttattaaaa ccaaattgat gttcaatttg tacactgagt gagtatgtta
     8521 aacctgatta agggggagtc atctgtggaa gatgtgaaaa aagtattatt aacaaaatca
     8581 aaacgttcaa aaatggaaca gcgtggaaaa ttcatcagtt ttttatgtat cgcgctgatc
     8641 gtgttggttg tattatctat tttttatttt gtagctagta aaggattggc cacctttttt
     8701 actgataaga tcaatgtatt tgattttctg tttggtacag attggaatcc aagccaaatc
     8761 ggagcaaatg ggaaaccaat ggtaggggca ttgcctatga tcacaggttc gtttattgtg
     8821 acctttttat ctgctatcgt tgccacgcca tttgccattg gtgcagcagt attcatgacg
     8881 gagatctctc ctaaaaacgg aacgaagatc ttacagccgg tcatcgagct gcttgtaggt
     8941 attccttctg tcgtttatgg ttttatcggt ttatcagtta tcgtgccttt tgtcagaagt
     9001 atttttggcg ggactgggtt tggtatctta gctggtacat ttgtgttgtt cgttatgatc
     9061 ttgccgactg tcacaacgat gactgttgat gcattaaagg cagtgccacg gcactatcgg
     9121 gaagcttcat tagcgcttgg tgccacaaga tggcaaacaa tctacaaagt cgtgctacga
     9181 gcagccgtgc ccgggatttt aacagcagtc gtttttggta tggctcgtgc gtttggtgaa
     9241 gccttagcta tccaaatggt catcggtaat gcagcattga tgccaacagg tcttactact
     9301 cctgcttcta cattgacgtc catcttaaca atgggaatcg gaaatacaat catgggaaca
     9361 gttgaaaata atgtgttatg gtcattagcg ctgatcttgc tattgatgtc attattcttt
     9421 aatatcgtga tccgtatgat tgggaagaaa ggagagctga aataatgaac gcaaaacgct
     9481 cagataaaat tgctacaggt gttttatata tagtttcggg gatcatcgta ttgattttag
     9541 cagctttact actttatatc ttagttcggg gaataccgca catttcatgg tccttcttga
     9601 ccgaaccttc caaagcctac caagaaggcg gtggaatcgg gatccagcta tttaactcga
     9661 tttatttgct tttgatcacg atgatcatca gttttcctat ttctttaggt gcgggtatct
     9721 atctttcaga atatgctgga aaaaactggc taacagatgt gattcgcacg tcgattgaaa
     9781 tcttgagctc gttgccttct gtcgttgtcg gtttattcgg tttcttggtt ttcgttattc
     9841 aattccagta tggtttctcg attatttctg gtgcattggc tttgactgta ttcaacttgc
     9901 ctcttttgac tagaacagtt gaggagtctt tacaagcggt ccattacaca caacgagaag
     9961 caggattggc cttaggtttg tcacgctggg aaacagtgat aaaagtggtg gttccagaag
    10021 cgacaccagg gattttgact ggtgtgatcc taagttctgg aagaattttt ggtgaagcag
    10081 ctgcattgat ctatacggct ggacaaagtg cacctgcgtt agattttacg aactggaatc
    10141 cattaagtgt ttcaagcccg atcagcattt tccgtcaagc tgaaacatta gctgtccata
    10201 tttggaagat caatacagaa ggcacgatgc ctgatggtgt ttctgtttcg gctggcgctt
    10261 ctgccgtgtt gattattgct gttttactgt tcaacttcgg cgctcgtgcg attggcaaac
    10321 gattataccg aaaaatgact tcagcctaag gagaagaagc aaaatgaaaa aatataactg
    10381 gaatgaaaca aatgtcatca ccatgccaaa agagaaagaa gtagcactat atacggatga
    10441 tctacatgtc tggtatggcg acaatgaagc aatcaaagga gtggatctgg aatttgaaaa
    10501 aaataagatc actgcactga ttggtccttc cggctgcgga aaatctacgt atcttcgttc
    10561 gttgaatcga atgaatgatg ggatcgctaa tacgaaaatc actgggaaaa tcatgtatca
    10621 aggcgtagat attaatgcgc ctcaagtgga tgtttatgag atgcgcaaac ggattggcat
    10681 ggtttttcaa cgtcctaatc cgttcagcaa gtcgatttat gacaatatta catttgcttt
    10741 gaaacagcac ggcgaaaaaa acaaaaagaa attagatgaa atcgttgaaa caagcttgaa
    10801 acaagctgcc ttgtgggacc aagtaaaaga cagcttggac aaaagtgcct tggctttatc
    10861 tggcgggcaa cagcaacgtt tatgtattgc tcgtgcgatc gcgatgaaac ctgatatctt
    10921 gctgctagac gagccggcaa gtgctttgga ccctatttcg acaggaacgg ttgaagaaac
    10981 acttgtaaac ttgaaagaag actatacgat catcatcgtg acgcataaca tgcaacaagc
    11041 agctcgtatc agcgactata ctgccttttt ctatttagga aaagtaatgg aatatgacaa
    11101 aactaacaag atatttacca gaccaaaaat tcaggcaacc gaagattatg tgtccggtca
    11161 ttttggttaa gacggaggcc tcaacatgac aaaagagatt atttcatcta aagatcttca
    11221 tctgtattat ggaaagaaag aagcactaaa agggatcgat ctaagcttca atcaagggga
    11281 aatcactgcg atgatcggtc cttctggttg cggaaaatcc acttatctgc gttctttgaa
    11341 ccggatgaat gacctgattc cgaatgtaac gatcaccgga agtgttttat ataaagacaa
    11401 agacatctat ggtccaaaaa cagatgttgt tgaactaaga aaagaaatcg gcatggtatt
    11461 ccagcagcca aacccatttc ctttttctat ctatgaaaat gtcatctatg gattgaaatt
    11521 aaaaggggaa aaagataaaa aagtccttga tcaagtagtt gaagaaagcc tgaaggcagc
    11581 gtctgtttgg gatgatgtga aagacaaatt gcataaaagt gctttatctt tatccggtgg
    11641 gcagcagcag cgtgtatgta tcgcacgtgt tttggcagtt aatccggaaa tcatcttatt
    11701 ggatgaacca acaagtgcgc ttgatcctgt ttctactggg aaaatcgaaa gtatgctgtt
    11761 agaattaaga gagcggtata cgatgatcat cgtgacccat aatatgtctc aagcttcacg
    11821 tatttcagat aagacggcat tctttttaga tggtcacttg atcgaataca atgatacgaa
    11881 gaagatattc atgaatccag aaaaacaaga gacagaagat tatatttcag gtcgatttgg
    11941 ataaggagga aacggaatgc tacgtacaca atttgaagaa gaattgctga atctccataa
    12001 tcagttctac gaaatgggga tgatggtgag cagcgccgtg cacaagtcag ttcgtgcata
    12061 catcaaacac gataaaatct tagcccaaga agttatcgaa aacgatataa atatcaataa
    12121 catggaaaca cgcttggaga agaaaagttt tgaaatgatc gcattgcaac agccagttac
    12181 aactgatttg cgtatgatca ttacggtcat gaaagctagc tcggatttag aacgtatggc
    12241 cgatcatgcg gtgtctgttg ctaaatccac gattcgcgta aaagggcaga cgagaatacc
    12301 ggaaatcgaa aaagagatct ccgatatgtc cgattatgtg aaaaaaatgg tggacaacgt
    12361 cctagttgct tatgtcaaaa cagacgaaaa agacgcacgt acgatcgcca atatggatca
    12421 aagagtcaat gactatttca atcgaatcta taatgcaaca atcaaaaata tgcaggaaaa
    12481 tcctgagaca gtcatcagtg gaacagacta cttgaatgtt gccagttatc tagaacggat
    12541 cggagactat gtgacaaaca tctgtgaatg gatcgtctac ttggctacag ggaaaatcac
    12601 tgagttaaat acgaaccaca atgagatttt ctaaactgaa gctgtgatta tgccgtttgt
    12661 ttaggaaaag aggttgtgaa atcagcggtt gtgaaaaagc tgttctgcat caagtaataa
    12721 gaacagtcaa acaaaaaata gctcctcata tttttcgttt ggctggactt attaccgcag
    12781 atgcaagctt ttgaacaccg tttattcggg aaagggtttg tgacaaaaca tgtcacaaac
    12841 ccttttcatc atttcataga aaggataaag aatgcaaaaa aaaattagca tcatcactat
    12901 attttttatt tgtgtgctta cttcatgggt tattcagcat caagccgctt tatatgaagt
    12961 acctgtcatc gagatagaaa aagtacgaat aaaagaggat catcaattga ttacaggaaa
    13021 actgatgaac acggatcaaa aaggagaatc gatttattta tcggttcctt atcaaaaaaa
    13081 tcaaatagaa acagcaaata taaaagcgag acaaatgtat ctagtccaaa tgcatggaga
    13141 tgaatggcag ctagtagcaa aaaaaaatga tggctggctt ttcttcttca caagtatctt
    13201 cattggcgcc atgttgctta ttggtggaaa acagggtcta tttgctttag gaagtctcat
    13261 catcaatatc gttttacttt tacttttgtt gctcatcttt actaaaacag aagggatttc
    13321 tctattaaat atatctatcc tgttcgtcgt tatagggatg attattagca tattggcttt
    13381 agatggatgg aatagaagca gtataatcaa aatttcagct gcagttacga gtgttttttt
    13441 agcatttttt atttgtcttt tgacaatgaa tcattggaaa gaccaaggac tgcgctatga
    13501 ggaactgaat tatctgacac gaccgtatcg ttctgttttt ctttctagcg tactgattgg
    13561 aacagctgga ggaacactcg ataccgttat cacagtcatt gcgacattag aagaattgac
    13621 taagcgacaa ccagaaataa cagcgaaaca gttatggctt tcaggaaggc aagtgggtcg
    13681 agatgtgatt ggctcgatga ccaatatcct tttgttcgtc tatatcagtg ggtcagttcc
    13741 atcgtttctt ttatatttag gaaatcaatg gtcgttcaaa gaaacgatag aacagcattt
    13801 atcattggaa tttttacgcg tcattgctgg cagcttagga atagtattat cgatccctat
    13861 ctcggtcctc ttttttctga ctgtaaggag gctgaaacga tgaatgtact tattatactt
    13921 gttgggattt tagcaatcag cgtgctactt attggcggta cgcaaggtat gtatattctt
    13981 ttaggcttat tcatcaattt agggatcttt ttcctgttgt tattcggtta tcatcagaag
    14041 tggccgatac tggcattgtc ggtcatagga tttttattga ttgctgtggt tatcttattt
    14101 tttatcaacg gttataatct aaaaatgcgt gcagcttttg tgagtatact gatcttttta
    14161 ttttgtttcc tactattgct tcctatcact gattttttag caatccaagg ttttacatcg
    14221 gtagaactag aagaattatc tggcttagat aagactttag cgattgattt tagattactt
    14281 acgagatcgt tgcttctgat tagtcttagt ggtgctgttt tagatgcgag tgtagcaatt
    14341 tctagtggga cattcgaagt atatcaggca aatccgcaat tatcttttta ccaattgcgc
    14401 cattctagtt tcgctattgc taaaaaaata ttggcgtcaa cagtaatgac acttcttttt
    14461 gcattcatgg gaagttcttt agcgttgatc ctctggttta ttgatttaga cattcctttt
    14521 cagcaagtga tcaatgaaaa atcatttgtt ctagaatata cgatggctat tcttaccaca
    14581 ttgtccgcac tacttgttct tcctttaacc tgcgtgacta cagcatatct ctttacaaaa
    14641 aagaaagaac aactcaagat ggagtaaaag ttacaactta agtcctatga catttttact
    14701 gaatcagtgc ataatatgga tgtaaggaaa agcttgcaaa caattttgct taaggaggca
    14761 atttccatga aagaaagaga acgtatttta gatttagtga aaaaaggaat cctttcgact
    14821 gaagaaggct tagatttatt agaaagcatg gcaactgaaa aagacgaaaa gcaaatcaaa
    14881 aaagaagcag acaaggtaac tgctagtcat aaagaaaaag atcaggcaag tcaattgatc
    14941 gataaacttg aaaacggcga agaagaaatt tctgagccag ttgatccaaa ggaaagagaa
    15001 agacaagatc aagaaaactt agaaaagatc ttagatgaac tagcaactga agcgaataaa
    15061 acttcggcgc gtctagatga agtaaatgct caattcgctg acaataaggc agcacgcaat
    15121 gagaaacagg aagcattgat gcagctgaat acaaaagaag aattaggcga gttgacagaa
    15181 gaagaattag cacagcgtca gacgttagaa gcagaaatca aagaattaga agctagtggc
    15241 gatacactgt tagaagaaca attgaaatta gaagcagaat taaaagatat ccgtaagaat
    15301 caatggtctg agaaaaaaga aacgtttact gataagtttg aattgccaga tgattggaaa
    15361 gatcaagcaa cagatacact gaatcaagta ggcgaaaaaa tgtcagaagc tggatcacag
    15421 ttaggcaaat tcttgaagaa aacattccaa actgtttcag aaacagtgaa tgacaacatg
    15481 gaatggaaag atgtcagctt acgtgtccct gggattgcga caacgaaatt tgagcatgaa
    15541 ttttactatg aagctcctgc agcaagtatc cttgatatca aggcagcaaa cggaaacgtg
    15601 acgttaaaaa cgtgggacag caacgatgtg aaagttgaag ctaagatcaa actttacgga
    15661 aaaatgggtg ctgaaccatt tgaagcattc tctgaacgga gccagattga ggttaatgaa
    15721 gatcatatct ccttccaaat tccaaataaa cgtgtgcgtg cagaccttgt tttctactta
    15781 cctaaacgag tatacgacca cgcagctatt aaacttctaa atggcaacat catgattgaa
    15841 acattggaag ctaaagatat ttatacaaaa tctacgaatg gtaacatcat cgtggaccaa
    15901 ttaactgcaa cgatgctgga agtagaaggt gtaaacggca atatcgatat tcgaaacggg
    15961 aacatcttag attctattat cgaaacagta aatggtacag tgacatttgg tgcgacagca
    16021 gaaaaccttt ctgtgtcttt ggtaaatggt gatgtacgtc ttacgattaa agaagataat
    16081 ttgaaaaaag tagaagcaag ttcagtgaac ggaaatgtca aagtcgcttt accagatggc
    16141 atcggtttgg aaggacatgc taaaacaagt cttggaagta tcaacagccg tctgtccaat
    16201 tacgaagtag ttcgtgagaa aaaagaacgg acaaaccaaa tgttgcaatt ccgtcgtgtt
    16261 tcagatggtg aaatcgcgca agtgcaactt tcaacgacaa caggaagtat ctatttaaaa
    16321 gatacagata aataaagagg ttgtgaaaga gatgttcagc cttgagcaat aagaagcgat
    16381 tttgtaaaat agcttcccct attttacaaa attgtgcctt attgccgata gtctatctct
    16441 tgaacaccgt ttaccaagag attatgaacc tgccaattat cgccgagtca caaggaataa
    16501 tttcagaaaa agctcgtcac gttttatgaa tatggtaatt tgtgaccgag aattttgcag
    16561 aaagacaccg attaagaaga aggttgtcac aacgattttc ttcatttaaa atagtagaac
    16621 agaggtgaga catgatgaaa aaaaagttga caaaatctaa taaaaatgtt gttttgacag
    16681 ggactttagc aggaatagga gaatacatcg ggattgatcc gactgttatc cgtgtgcttt
    16741 atgtattcct gagcttagtt gcttttggaa gtccaattat tttatacatt ttattagctt
    16801 tgattatccc atctgcgcgt tctgcacgaa caaactatgg acataacaat gcatattatg
    16861 aaaaaaacaa ttatcgtgaa caaactcgtg aacaaacaaa aaaacgcaaa gaagcggaaa
    16921 aaatcgatga tgacgaatgg agtgactttt aatgtcgtac tttcaacgta ttttagttaa
    16981 tacgttaaca ttcatatcat tggctgttct ttttcctgcg atggtccatg tgagcagtat
    17041 ttggacggcc gttcttgcca gcttcgtatt gtctgtgttg aatatgtttg tcaaaccgat
    17101 tttgacgata ctgtccctgc cctttacatt agtcacattg ggattgttta cgtttgttat
    17161 caatgcagcg attcttaaaa tgacctcctt ttttgtggga gcagaacatt ttagtttttc
    17221 ttcattcggt gcagcgttgt tagtagctat cttgatgtcg cttgtcaatg taattgtcag
    17281 cgaacataac agtagaaaat aaaagaaagc tgtgccgaac tatcggcgca gtttttttat
    17341 ttagaatcag gaatgcgttt tctattacat tggtaattaa ataaaaacgt tttttttaat
    17401 tggtagtact tttggctaga caatgataaa atgatagaga acactctaat agaaatgagg
    17461 tagcatgaat gtcagaagtt gtaaaagtta gccaattagt gaaaaaatta tctttggaaa
    17521 tcgtgacagg cgacgagaaa agcttagacc gtgtcatcgt aactggggac atctcacgcc
    17581 caggactaga gttgactggt tattttaatt attattccca taatcgttta cagctatttg
    17641 gcagtaaaga aatcagtttt gcggaacgga tgatcccaga agagcgtttg atcatcatga
    17701 gaagattatg tagtgaagat atgcccgcat ttattatttc aagaggatta ccagctccag
    17761 aagaaatgat ccaggcagca aatgaaaatg ggatagctgt actgcgttct cccatctcaa
    17821 cttctcgttt attaggagaa ttatccagtt atttagatgg tcgccttgcc cctcgaacaa
    17881 gtgtccacgg tgttttagtg gatgtttacg gattaggtgt actgattcaa ggtgacagtg
    17941 ggataggaaa aagtgaaaca gcgttggagt tgatcaaacg cggccatcga ttgatcgcag
    18001 acgatcgagt agatgtttat cagcaagatg aattaacggt gatcggagaa ccgcctaaaa
    18061 tcctggaaca tctgattgaa atccgaggaa tcgggatcat tgatgtaatg aatctttttg
    18121 gtgcgagtgc ggtacgtggc tctatgcaag tccagttggc tgtttatctg gaagcatggg
    18181 caaaagataa gaaatacgac cgtctgggct ctgaagacac gaccgttgaa atcgcggaag
    18241 tgggcattcc acaaataaaa atccctgtca aaacaggacg aaacgttgca atcatcattg
    18301 aagtagccgc gatgaatttc cgtgcacgta caatgggctt tgacgcaacg aaaacatttg
    18361 aagaaagatt aagtcgcttg attgaagaaa actcaggcaa cgactagcaa ggagtgacaa
    18421 tatgttagca caaatcaatc gagttgcttt tgagcttgta gggattccta tctactggta
    18481 tgcattgatc atcgtatcag gtatcattat cgctatgtgg cttagcagca gagaggctgt
    18541 tcgggtagga ttaaaagcag atgatgtgac agattttatg ttgtggggat tgcctatctc
    18601 cattattggc gctcgcttat attatgtttt atttgatctt ccccagtata tagctgatcc
    18661 aatccaaata ttcaatatcc gttcaggagg cttagctatt tatggcggac tgattgcagg
    18721 cgcgatcacg ctgtattttt ttactaaata tcattttatt tctttgtgga cttttttaga
    18781 tatcgctgca ccaagtgtat tattagcaca ggcaatcggt cgttggggaa atttcatgaa
    18841 ccacgaagca tatggaccag acacgacgcg aagcttctta gaaagtctgc atttaccgca
    18901 attcatcatt gataatatgt atatcgaagg gatctatcgt caaccaacat ttctttatga
    18961 atcactttgg agtttgattg gatttatttt gcttttgttt ttacgaaaga aaccacacct
    19021 tttgaaaaaa ggcgaagtcc ttttatgtta tctaatgtgg tatagtttcg ggcgtttctt
    19081 tattgaagga ttacgtacag acagtcttta ctttttaggt gtgatccgta tttctcaatt
    19141 attatctgct gtgttatttg tcggtacatt gattctattt atttggcgca gaaaaaaagg
    19201 aaatttaccg gattatgacc gctcattagg aaaaaatcaa caaattatct gatttttaga
    19261 gcgttaaaaa tgaaaaaaat ttcaggagtg gtaaaatgaa ttatagagta gcggttttag
    19321 gaccaggctc ttggggtact gctttggcac aagtcctcgc cgaaaacgga catgacgttc
    19381 ggatttgggg acatcatgct tcacaagtag aagaaatcaa cacccaacac acgaatcaac
    19441 gttatttacc agatcattat ctacccgatt ccatccaagc attcaccaat atggaagatg
    19501 cagtaaaaga cgcagatgcg gtgctattcg tcattccaac gaaagcaata cgttctgttg
    19561 caaaagaact agtgcctaaa ttgaatacaa agccagtgat catccatgca agtaaaggat
    19621 tagaacaggg aacacataaa aggatctctg acatcttgtt agaggaaatc ccagcagaaa
    19681 aaagaaaagc tgttgttgtt ctttctggac caagccatgc agaagaagta gcaaaacatg
    19741 acatcactac gatcacagct gcaagtacag atgcaaaagc agcaaaatat gtacaagagc
    19801 tgttcatgaa taattatttt cggatctata ccaatcctga tgtgatcggt gtagagatgg
    19861 gggcagcttt gaaaaatatt attgctattg gtgctggcgc tattcacggc ttagggtttg
    19921 gcgataatgc aaaggcagca atcatgacgc gcggattagc tgagatcagc cgtttaggtg
    19981 tagcaatggg tgccaacccg ctgaccttta tcggattaag cggaataggc gatctagtcg
    20041 ttacttgcac aagtgttcat tctaggaact ggcgcgcagg caacttgtta ggacaagggc
    20101 agcccctaga agaggtgcta gccaatatgg gaatggtcgt tgaaggtgtg aatactaccc
    20161 aagcagcgat ggaactagcc caaatgatgg aaatcgaaat gcccatcact caaacaatct
    20221 atgaagtgct gtatgaacaa aaagaaatca aaacagccgc aaaagaaatc atgttacgtg
    20281 atggaaaaaa agaaaatgaa ttctcattaa caattttttg aaggagcgaa tgtatatgaa
    20341 agttaaaaaa gcagttattc cagcagcagg attaggaaca agatttttac cagcaacaaa
    20401 agctatggca aaggaaatgt tgccaattgt tgataaacca acaatccagt tcatcgttga
    20461 agaagcgttg aaatcaggta tcgaagacat cttgatcgtt acaggaaaag ctaaacgacc
    20521 aatcgaagat cactttgatt caaatattga attagaaaca aacctaagtg aaaaaggaaa
    20581 gaccgaatta ttgaaattag tagaagaaac gacagatgtc aacttgcatt tcatccgtca
    20641 atctcatcca aagggtttag gacatgccgt tcttcaagca agagccttta ttggaaatga
    20701 accatttgtc gttatgctag gcgatgatat aatggaagac gaagtaccat tgacacagca
    20761 attgatcgat gactatgagc gtacacacgc ttctacgatt gccgttatgc aagttcctca
    20821 cgatgaaact tcaaaatacg gaatcatcaa cccaggggaa gtactggaag atgggttata
    20881 caatgtcaaa aactttgttg aaaaaccaga tccaagcaaa gcaccaagtg atttagctat
    20941 tattgggcgt tacttattga ctccagaaat ctttgatgta ttatcagaac aacaaccagg
    21001 tgctggaaat gagatccaac taactgacgc aatcgatacg ttgaataaaa cacaacgcgt
    21061 atttgcccgt aaattcacag gtaaacgtta tgacgttggt gacaaatttg gttttatgaa
    21121 aacaagtatt gaatatggtc tgacacatcc agaagtaggg gcacctttgc gtgaatacat
    21181 catgtcttta ggtaaaacat tagaagcaga aggaagcaag atcaatcata atccaaaagg
    21241 aataacagaa atcaaagaag aaaagaaaaa ataatctttt tttctaacga gcttcagcgg
    21301 ctaattgaat aaaaagtagt tgaagctcgt ttttatttgt ttaaaaaaca cgaaaatgct
    21361 atccttatag aaaagagaga caagaaggga gtttgatcat gtcaggagga gaaattgcag
    21421 gattgatcgc agcaatcgcc tttgcgatac ttgtcgtttt catcgttcgc gtattgttgc
    21481 aagtttccaa aactttagaa aaagtagacc agactgtcgc agaagcaaat acgacaattg
    21541 agatcgtcac acaagacgta gatcttttat ctagacaagt agaaggcttg ctagtcaaaa
    21601 gtaatgagct gctcttggat atcaatggaa aagtcgcaac gatcgatcca ttgttcacag
    21661 cagttgctga tttaagtgaa agtgtatcgg aacttaatca ttctagtaaa aacatcgtga
    21721 cgaaagtcgg cggaatcgga aaaacgacag ctaaagcgac tgttgccgga aaagtcggtg
    21781 gaacagcgat gaaatttttt aaaaacaaaa aacatactga aacgacagga ggaaaataag
    21841 aatggctaaa aaaggtggat ttttattagg tgcagtagtt ggaggtacag ccgcagcaat
    21901 cgccgcatta ttgttagcac ccaaatctgg taaagaactt cgggatgaat tggctgctca
    21961 agcagatgat ttgatggaca tggcttcaga ttacacagat atggcaaaag aaaaatcatc
    22021 tgagttaaca ggtattgcaa aagaaaaagc gcaaggtctt tctcaacaag caggggatct
    22081 agcaggcaat gttaagcaga aggtaagtga atcagcagag gaagcaggaa gtgaatcaga
    22141 tgatatcttg ttaaacctca aagaacaatc agctgattta tctaatcgtt tcaaaaaaac
    22201 agcagaagat ctgaaaaagc cagcagacga attaggtagt attgctaaag atacttcaga
    22261 agatatcatg atcgatgtaa aagatgctgc agataaagca ggagagacgg tcaatgaagg
    22321 aatgtcagaa gcaaaaccag ttgcagatga cacaaaagaa atgttgaaaa tggcaaaaga
    22381 agatgcaaaa gaagctaaaa aagaaatgaa agatacaatg acagattcga ctaccaatca
    22441 ctaagattac tcatttctag aataaaaaag gtgagagcgg accagctctc acctttttta
    22501 ttttcattac tctaaaacaa gtaattcttt tggtgtagtc gtgaatggca cacaaccatc
    22561 acttgtcaca tacacacaat cttcgatacg tacccctact ttttcaggaa tatagattcc
    22621 tggttcaaga gagaagcaca tcccttcttc aatcactaag tcgtttcctt ctacaagaga
    22681 agggaattcg tggacggttg ttccgattcc gtgacctaaa cggtgattga aatattcgcc
    22741 gtagccatag ctgttgatca cgtttcgtgc gatttgatca agttcacctg ctgtcacacc
    22801 tgggcgaact gcctcttgtg cggcaagctg tgcttcaaga gtgatgttat agattttttc
    22861 ttggaactca gttggttttt gataagcaac tgtacgagta gcatcactgc aatagccatt
    22921 ccaaataacg ccaagatcga ataacactag ttcatttggt gaaaccttcg tgcttcctgg
    22981 tgtaccatga gggcttgcag catttgctcc tgcaaggact aatgtgtcga aagacatttg
    23041 tgaaacacct tgttttttta attggtattc gatttctgcg ataattgctt gttcagttac
    23101 gccttctttt actgttttga agccaatttc aaaggcaaca tcggcccatt taccagcttc
    23161 gatcagtttg tctttttctt ctaccgtttt gatcagttgc agacgctgga taatagaagt
    23221 tagatcggtg taagaatctg tacctgtcaa gatatcagag atagcatcga agcgatcgac
    23281 agtcagagca ttcttttcaa tacctacatt accaggttgc ccatagcgtg cgcgaagctc
    23341 ttttccgatg atctcccaag gattttcgct gtctaagtag ccacgtacat cgtacgccca
    23401 gctgctgttt tgagcatctt caacttccag agcaggagtg aaaaggaaag gatcttgttc
    23461 cagagggata aaaagagcta gtacacgctc gtgaggatca ctttcatatc cggaaaagta
    23521 agcgatgtgg ccagggtcgc tgatataagc caattctact tggtgttgtg ccatccattg
    23581 acgtagttca ttgattttta cgttattcat ttggcaacct actttctaaa ttaattttgt
    23641 attcaataat tgtaccactg attgattaaa agaccaacgt tcttaaaact gaaaaaagta
    23701 aaaattttca tgaaaacaga gataaaacgg ttgcaattgt tgagattatt tgatatccta
    23761 ttttagaaat gtaagacgta ttttcagaaa acatataaaa tatgaatagg agaacacaaa
    23821 tggaaaagca aacaatcact atctatgatg tagcgagaga ggcaaatgtt tcgatggcta
    23881 ctgtttcacg tgtcgtaaat ggcaatccaa acgtcaaacc agcaacaaga aaaaaagtct
    23941 tggaagttat cgatcgcttg gattatcgtc caaatgcagt cgcacgtggc ttggcaagta
    24001 agaaaacaac aacagttggg gtaatcattc ctgatgtcag caatatgttc tttgcctctt
    24061 tagcacgtgg tatcgacgat gtggcaacaa tgtacaaata caatatcatc ttagcaaact
    24121 cagatggcaa cgatcaaaaa gaagtcaatg tattgaataa tttattggct aagcaagtgg
    24181 acggcgtgat ctttatgggt caccatatca cagacgaaat ccgtggagaa ttttcacgtt
    24241 caaaaacgcc tgttgtacta gctggttcca ttgatccaga tgaacaagta ggtagcgtaa
    24301 acattgatta tgtgtcagca acaaaagatg cagtaaataa attagcaaaa agcggcaata
    24361 aaaaaatcgc ttttgtcagc ggtgcgctga ttgatcctat caatggtcaa aaccgtctaa
    24421 aaggctataa agaagcatta agcgaaaaca atctaccata tagcgaagga ttgatttttg
    24481 aggcacaata caactttaaa gatggtctgt ctctagttga ccggatccat aatagcggtg
    24541 caacagctgt ctatgtttca gacgatgaat tagccattgg tatcttggat ggtttattag
    24601 accgcggcgt aaaagtacca gaagaattcg aaatcatcac aagcaacaat tctttactga
    24661 cagaagtagc tcgtcctcgt ttgacaagta tcacacaacc attatacgat ttaggagcgg
    24721 tatccatgcg cctattgacg aaaatgatga ataaagaaga agtcgaagaa aaaacgatta
    24781 tcttgcctta tggaatcgaa gaaaaaggat caactaaata ataaaagatc aatcatttaa
    24841 aggttgttcc aaaagtggtc aacttctgga acaacgttta tacgagaaag ggggttatga
    24901 caaaattttg tcataacccc tttttttttg tgactttaaa aaaagagaaa atggcggaaa
    24961 caaaccttac gtccatcgta tacgacacaa tctgtgcgta gatgctacat tttgttcact
    25021 actatcaaaa ttactttaaa atgaaaacgt gataaaaaag aaagtagaaa agtagataag
    25081 taactctaaa aaatccaaaa aaatccaggc tcgattgcgt acttatctta aaggagaaac
    25141 aatccatcat atataacccg aaaggagact tctatttgcc taagaaaaga cgaaagacca
    25201 tagtcatcat tttgaccatt cttactatag gaatcggagg gaaattttac atggacaaaa
    25261 gagaaatgca aagacagaaa gatttactcg cagtagaaaa acagtccgtc aaagttctga
    25321 aaaatacctt tgcggatatc aaggaagtga agattgaaaa atctgctcga aatgaaatga
    25381 cgggttcata ccggatagtt attctaatga ctaataaaca agatcaatcg atttattttt
    25441 cttatagttt ttggaaagaa agaaatgaga taggaagtta cggaatagtt gatgaaaaaa
    25501 agcaaaaaga aggaaatacg ctcaataaag ttaaagtaac ttactctaat ggaaatgagg
    25561 aaagtattta atgattactg acaaacaata tagtgaatta agtgatgctg tttactggct
    25621 agatcctaaa catagagact atgtacctga aatgcaagaa aatttaagtt ttaaaattaa
    25681 cggcactatg tataaaatcc taaaaatcaa aaactccttc gacggcatgc aggcgatggc
    25741 tgtagcgcca atcgtccata gcaaattgga gaaaaatttc aaaaataaaa aaattcctgc
    25801 aaactttcgc gtacttaaat aatgaaggtg tcaatactta cataaaaaga aaggaggcac
    25861 gttcttgtca aggaaaataa gaaaactcag cttagccatt ttattaatcg tcatattagg
    25921 gatcggaggg aaagtctata tggacaaaag agaagcacaa aggcagaaag atttactcgc
    25981 agtagaaaaa cagtcagtca aagttctgaa aaataccttt gcggatatca gggaagtgaa
    26041 ggtagaagaa tttaaaaaaa accctgttac cggaagttat ggtgcacttg tgacaatgac
    26101 aaataatgaa gggaaatctg tttattttga ttatagtttt tggaaggaga gaaatgaact
    26161 tggtggtttt ggcattgaga acagagcggt acaaaagaca ggagtcacta taaaaagagt
    26221 aaaagtaatt ttttctaacg gacaggagga gttagtatga caataaacga taagcattac
    26281 aatgatatta gtgagaaggt ttattggcta gatcccaaat atccccgcta caatgaggga
    26341 tacaaaaaga actctgttaa agaatttgct ggtatggaat ttcaaatcct acaaatcaaa
    26401 gactcattgg acggcatgca ggcgatgtct gtagcgccaa tcaagaacgg taaagtggat
    26461 ctttctcaag tagtgattgc ttttgcagga acgaacttaa gtgattggaa agatttggaa
    26521 acggatgcaa gaagtatcat gaatctctac gggaatagac caggagcaag tttttctgaa
    26581 aaagcaacaa caaacaagat tttatccgtt agtggattga aaacagcggg gcaaatcaat
    26641 tcagcgattg catttgctaa tgaagtaaaa aaggaatatg gagaagctgc tatttctact
    26701 accggccatt cattaggtgg attcttagca ttatatgtag cagcagagaa acaatggaaa
    26761 aatgtcggct tcaacgggcc ggacccgtat gatctcttat caccagaagc aaaaaaatgg
    26821 gtggaagaaa atcccggtat gctcaccaat taccgaaatc acgccgattt tatcggtaat
    26881 tttggtggta atggcacagg tgctgaagtt cggatcagta tggaaacagc attcttgaat
    26941 ctgcttgctt cccacagttt aacctcttgg aagtttgatg aaaatggaaa gctgattatt
    27001 cctgaaaatg aaaacaatgt taaagctcga aaacaacaga aagaaaacca agcgacccag
    27061 ttgttttcct atcagttgca agaactgaac aacatacgta aaaaactacg attaagtggc
    27121 ggccagctga cagttaatga acaaatttac ttagatgaca cacaagctag gttagtagtg
    27181 gaaaagttat cggcaaaaat gaaaagcgct atggaagaag ttatccgcat caatcaaaga
    27241 ggaatttttg agctagagaa aaaatggcaa gaaggcttgc ggcaagcacg tgttgtagcc
    27301 ccttcgttga ctgaacagga aatcatagaa gcattagcta gtgtgggcgc cacaaaagaa
    27361 aacttagtcg attctgcgaa agaagtctat ctgctacgaa tcaataaggc aagacgtatg
    27421 ggggaggcct ttgatacatt ggcgaaggag atccaagaca aaatcaatga aatcgtggca
    27481 cgagatcgag aattagcaca gcaattcaat taaaggagaa gtagatgaaa ctagaagaat
    27541 tggaaagaga ttatcgaagt gaacgaagac aattagaagc tcaagaagat gaattgatcc
    27601 actttaagaa tcaaggatta cgattgatgg agcaagagga gcagcgactg catcatgtat
    27661 taaagaactt agctttagat aatgagcctc tgtttcaagc aagacgaagt tattcgaatt
    27721 atcaggagca ttatcaacaa gccctacaag tagaacgacg aaaactctat cagcagcaag
    27781 atgaactaga acgttcctat cgtcaaaaaa tcagacaaat aactatggag aaatcatctt
    27841 atgaatgaat ttttaatgca attactaaaa gaattagaga aaaatctaca tcgatcaaat
    27901 gtacgttcag ttggtatggg gatatctcag caaatttggg cagaaaaacg attgacagat
    27961 caaaactacc aaatgactgc agaagaagct gctcaagaat tttctaaccg acatgtacac
    28021 ttgaaaggat cagtaaaagg gcaatttgca gaaaaactaa ctgaattatt ccaaagacaa
    28081 gaaaagaaat taaaacagtt tcgttagtaa agttatatag aaaaattttg tgaaaat
//
LOCUS       DAFEPQ010000032        25945 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.032, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000032
VERSION     DAFEPQ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.032"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(87..2354)
                     /gene="gshAB"
                     /locus_tag="LP543_001509"
     CDS             complement(87..2354)
                     /gene="gshAB"
                     /locus_tag="LP543_001509"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBL2321679.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEAIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(2541..3539)
                     /gene="ftsY"
                     /locus_tag="LP543_001510"
     CDS             complement(2541..3539)
                     /gene="ftsY"
                     /locus_tag="LP543_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBL2321680.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKIEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(3551..4360)
                     /locus_tag="LP543_001511"
     CDS             complement(3551..4360)
                     /locus_tag="LP543_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL2321681.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMVHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(4369..7950)
                     /gene="smc"
                     /locus_tag="LP543_001512"
     CDS             complement(4369..7950)
                     /gene="smc"
                     /locus_tag="LP543_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBL2321682.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEVAEKETEIQKAEASLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYVQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(7970..8656)
                     /locus_tag="LP543_001513"
     CDS             complement(7970..8656)
                     /locus_tag="LP543_001513"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBL2321683.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(9004..10785)
                     /locus_tag="LP543_001514"
     CDS             complement(9004..10785)
                     /locus_tag="LP543_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL2321684.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(10811..11725)
                     /locus_tag="LP543_001515"
     CDS             complement(10811..11725)
                     /locus_tag="LP543_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2321685.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(11752..12714)
                     /locus_tag="LP543_001516"
     CDS             complement(11752..12714)
                     /locus_tag="LP543_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2321686.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(12714..13658)
                     /locus_tag="LP543_001517"
     CDS             complement(12714..13658)
                     /locus_tag="LP543_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2321687.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(13659..14663)
                     /locus_tag="LP543_001518"
     CDS             complement(13659..14663)
                     /locus_tag="LP543_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2321688.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(14931..15173)
                     /gene="acpP"
                     /locus_tag="LP543_001519"
     CDS             complement(14931..15173)
                     /gene="acpP"
                     /locus_tag="LP543_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBL2321689.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(15295..16296)
                     /gene="plsX"
                     /locus_tag="LP543_001520"
     CDS             complement(15295..16296)
                     /gene="plsX"
                     /locus_tag="LP543_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBL2321690.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(16345..18381)
                     /gene="recG"
                     /locus_tag="LP543_001521"
     CDS             complement(16345..18381)
                     /gene="recG"
                     /locus_tag="LP543_001521"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBL2321691.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(18498..20180)
                     /locus_tag="LP543_001522"
     CDS             complement(18498..20180)
                     /locus_tag="LP543_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBL2321692.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(20232..20594)
                     /locus_tag="LP543_001523"
     CDS             complement(20232..20594)
                     /locus_tag="LP543_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL2321693.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            20864..21052
                     /locus_tag="LP543_001524"
     CDS             20864..21052
                     /locus_tag="LP543_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBL2321694.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            21290..23959
                     /locus_tag="LP543_001525"
     CDS             21290..23959
                     /locus_tag="LP543_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBL2321695.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(24050..24670)
                     /locus_tag="LP543_001526"
     CDS             complement(24050..24670)
                     /locus_tag="LP543_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL2321696.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            24782..25183
                     /locus_tag="LP543_001527"
     CDS             24782..25183
                     /locus_tag="LP543_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321697.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANS"
     gene            25318..25656
                     /locus_tag="LP543_001528"
     CDS             25318..25656
                     /locus_tag="LP543_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HBL2321698.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         7027 a         5500 c         4644 g         8774 t
ORIGIN      
        1 acgaaaaaac acataaattt ttacatgcaa aaaagctttt gatcagattg acatcaaata
       61 ggacaatctg tcaaaagctt tttcttttaa aatacttcag gatataacaa tttcaataca
      121 ttcatcgtca atcttctgcc ttttcctgca aagggataaa catgcatatg catggctgga
      181 ttgaaatttg cttcgatgat cccatatgct tttttatcag ttgtaggatc gatttcttta
      241 tctggtataa tcaaatcgat accactaatc ttagctccta gtgcttctac cgcagaaacg
      301 gcgatttttt tgtaagcttc agaaaattca tcagtcatat cgatcgaatc accacctgta
      361 ctgatatttg agttctccct tagataaaca atttgattgg cttggggaac tgattcgatc
      421 gtcaaccctt gttctttcaa catcaattgt tctaattcac ctaattggat caattctaaa
      481 ggtgcacgat gattcgttcc acgcaaaggg tcactatttt tttcttcaac aagttctttc
      541 actgttcgga taccgtcacc aataacatta gcagggactc gcaacatgat ggcttggact
      601 tctccgtcga tcacaaagaa acgatattcc gttcccggca taaattcttc tactaaaacc
      661 gaactgtctt cacgaaaagc aatggctaaa cctgcttgat aatcttccag actagctcct
      721 tccttgaaaa tcgtgatacc taacccgtaa ttggttgatt taggtttgat cacaaatgcc
      781 tgctcggcaa atctcggata agcttttacc gcttcttcca tggatgaaaa ttctgcgcct
      841 cctggtaccc ggaacccagc ttcttttaag acttttttcg ttactgtttt gttttccatg
      901 ataagtggga cgatatagct gtccttactt gtcatgtttg catttttcac atattctaca
      961 tggtcttgga attgcagacg caaaaattga tccgactcat ctaatacttt gacttggaca
     1021 cctttttgga tggcatcgaa cataaatatc tgagtagaaa gttccatttc gcgaaaaccg
     1081 gccaactgat agggacgttc atggctttct ttataatatt gatgtcccaa tgccacagca
     1141 acttcttgct gactcgatag ctgtgcttct tgatagagtt tcgctgctaa cgtttgttca
     1201 ggatgatcca tttgagtcaa tgcttcctct actaaataaa gagaatccaa ccaatccaat
     1261 tcaaccagca tttgcttcat cccttcaaga atttctctac cttcttgatg aaattctgtt
     1321 tgatctgatg gttgttctag tgctactttg ttatttcgag tcgtaccttc tgctacccat
     1381 tggtcacagt cttctttttc ttctgtccat aacatgtaca tcaggaataa ttgtaagaac
     1441 cgaacttgct ctggattgat acccaatctt gcataaggat tcaaatcgat attccgcaat
     1501 tcaatataac gaacacctgt tgttgctaaa tctgcgactt tctttccgcc tcggaaacga
     1561 agcggtgtat aaaattcttt ctcctccgaa agtttccctt cttcgatcat tcgctcgata
     1621 tctgctaaat actgtttcat agaagcatag gaaactttca catttggatg attcgtatag
     1681 cccagcgcac tgttacgaat actgcgaaca ggttcttgcg gatgtgattc atctaaaaag
     1741 tagttcttct cactcattgg tgaagcaccg aataaataag tgatcatcca gcgatagcgc
     1801 ataaagtttc tcgccgtttt caaataaagt tctgttttga attcggaaaa atcttgaaat
     1861 tcttcctgat ggctgaacaa tgttcttagc aactcatcgc caaattcaaa attgaaatgg
     1921 ataccgctta ccatctgttt tcgcttcccg tattcttttg ctaaataacg tcgatacaag
     1981 acatcttcga aattttttaa ttttgcaata atgatcgctt cgtctttttc cggtaaggca
     2041 ggtggcatac ttaatggcca gatcatctct tctttcggta tagaacgagc agtcacatca
     2101 taaacagcag cgagatactg aaataattcg ggaatagaat cggtaacagg agtgatcatc
     2161 tctgtttgtg tttcgctaaa atctgtttgg atataggggt gataggatcg atcgccaaag
     2221 attgctggat gatcggtttt tgctagattt cctgcaagat caacccgctg tccttcccgt
     2281 tctataccaa atcgagcttg gtcgataaaa gggcgcgcat tgacatgcaa taataattgc
     2341 ttaaaattca tcattaagtg acctccaatt catatgaatc taattataat caaaaagtct
     2401 aagaaaaaga acgaaattgt ttgagaaccg cttacaaata agtataaaaa tgaaggcatc
     2461 aaaaacacag ctatgttaaa aacaatcatt caaacaagga gctgcaactt ttgtcacagc
     2521 tcctgctttt tagacatggg ttattcttct tttagtaatc ctttgaacaa tccaacgaca
     2581 aaatcatttg gatcaaacgg ctccaagtca tcgattcctt caccaagacc gaccaatttc
     2641 actggtaaat gcaattcatt acggatagca agtacgatac cgccctttgc tgtaccatct
     2701 aattttgtca aaacaagacc ggtcacatct gttgtttctt tgaattgttt tgcttgaacc
     2761 atcgcgtttt gaccagttgt tgcatctagt acaagaagtg tttcttgggg tgcttctggt
     2821 aattcccgct ggattactcg ttttattttt tctaattcgt tcatcaagtt cactttattt
     2881 tgcagtcggc ctgcagtatc aactagtaaa acatctgcct gctctatctt tgctctttcc
     2941 aaagcatcga atacgacagc tgctggatcg ccgcctgcat tgccacgaac aacttcaaca
     3001 ccagcgcgtt cgccccagac aactaactgg tcgattgctc cagcgcggaa ggtatctgct
     3061 gctgccatca atactttttt accttcgttt ttgaactgat gggcaagttt accaatactt
     3121 gttgttttcc ctacaccatt aacaccgaca aacaaaataa ctgttagtcc gtcttcttgg
     3181 atatttagtt catttacttc attgattcct tccgcttcat agagatccac aagtttctcg
     3241 atgatcgcat tttggacttc tgccgatttt ttcgcatttc tgagtttcac ttcttgacgt
     3301 aatgcttcgg tgattcgtat cgctgtttca aaacctacat ctgcaccgat aagtgtttct
     3361 tctaattctt caaaaaagtc ttcatcgact gttcgaaaat tggcaaacaa ctcattcatc
     3421 cgttcaccaa atgttttacg cgtcttttct aaacccttgt cgtacttctc ctgtacttca
     3481 acttcttctg ttttctcttc gccagtaaaa gcacgcttga ttttatcaaa aaatcccatt
     3541 tgactccccc ctactctaat acaaattttt ggatcacttt ggctacgcca ttttcttcat
     3601 tgctggctgt cacgaaatct gctgcttctt tgacgaaagg cacagcattc tccatcgcta
     3661 cgcctagtcc agcgtattgg atcatcggta aatcattttc ttcatcaccg attgccatga
     3721 tttcttcttg tttcaaacca agatcttttg ccaataaaga aataccataa gcttttgtaa
     3781 caccttttgg cataaattcc aaaagcatac ttctcgtttt gatgatctca tacatttcat
     3841 ggaattctgc tggtatttct ttgattttag gatctaattc ttcttggtca taagcgatta
     3901 ctgccttatt aagcacaaat tcatctgtaa tatcgcttat atccgctggt tggaattgca
     3961 gcagcttatt caacacattg tacaaggatt gcttccttgg ggcactaggc agctgtaaaa
     4021 ccacattgtc agacaaaaca tctagtggca gatccaattg ctgagccagt ttgtataagc
     4081 gttggatatc tgatacttcc atcactttct tttccatgat ttcaccagta tcattttttt
     4141 gtaccaaacc gccgttgaaa gtgatgctgt agtcaccttc atctaccagt cctaattctt
     4201 gcagataatg caccatcgca gctaaggggc gtcctgtaca tagaacgatc ttgacccctt
     4261 gctgttttgc ttgtgctaat gcttgtttat tctcatctga aatcagcttt tgttcattta
     4321 ataatgtacc gtccaaatcg attgctacta atttgatcat tttcgttcct acactttcgt
     4381 ttcgttgatt ttcgcctctt cattgatatc ttccatccgt acagaaacga tttttgatac
     4441 accggattcc tgcatcgtca ctccgtacag tacatctgct gcgaccatcg ttcctttgcg
     4501 gtgtgtgact acgataaatt gtgtgtcatc ttggaaatcg ctcaagtaac gaccaaaacg
     4561 tttcacgttg gcttcatcta acgctgcttc tacctcatct aagatacaaa acggaacagg
     4621 acagacacgg ataatagaaa agagcaacgc gatggctgta agtgctcgtt ctcctccaga
     4681 aagcaaactt aaactttgca gttttttgcc aggaggttgt acttcaatct caatacctgt
     4741 tttcagcaaa tcagttggtt ctgtcagtac taattcagct ttaccgccgc caaacatatt
     4801 tggaaagaca attttgaatt cttgacggat agcttcaaat acttctttga atcttgtccg
     4861 cacttcatca tccatctcat ccatcgtttc aaacaattgg tttttcgctg ctaacagatc
     4921 atctcgctgt gttgctaaga atgtatgtct ttcactgact tgttcgtatt gttcgattga
     4981 atttaagttt actggtccca gtttttcaat ctgttctttt aaggatgaaa cctttgttct
     5041 actgtcttcg atatcggttg tttcttgata gtcttgggaa gctttctcaa atgtcagctg
     5101 atattctgac tgcagatagg tcaaatgatt atctaaaatc atttcagctc ggtctttttg
     5161 gacttctaat ttggattgtt ccgacagtcg tgctttttgc tcacgattac gctctgccaa
     5221 gactgcttca agcttatcga tctccttttg ccttctatct cgctgctctt tggcttttgc
     5281 caactcggct ttcaatgttt ctcgctgagc agataattcg ttaatttgtt tttccaaact
     5341 ttcttctgtg acttcatgat cagaaaagtc tgctgtcaat gtcgccaact gcttttctat
     5401 cgcttcttga cgttcggttg cctctgcttt ttggactcgg gcaccgcgga gctggatctg
     5461 cagatgatta aactgctctt tcaagacagc aagatctgct tgttcttgtg ctttttgact
     5521 ttgtacttgt gctcttctgg cttccatctg gtcgctttct tgacttaacg atttgatttc
     5581 ttcgtcgatt ttttgtcgtt gcgattcaag atctgtttgt ttgtctttca attcgttcgt
     5641 ttgcttttcg taatcttcga taaactgctg cagttcacgt gtctcaaaag ttgagatttg
     5701 cttttctttt tcaaaacgtt caagttcatt tatgatgttt tgcagctgat ttgtcgcttc
     5761 ttgttcttca aaacgtaatt gttcgcctcg tgtacgcaaa acttcttgtt cttcggctaa
     5821 acgagctgtt tcttgttgca gctcctgaac ttttttttct tgtgcttgga gttgtttatc
     5881 tgcttcttca aattcactag tcaactgttt caattcttga ttttgtacaa acaaactgcc
     5941 ttggtttcct cgtttattcg ctccacctgt catggatcct ccggcattca tcacatcacc
     6001 ttctaaggaa accactcgat attgatagcg gatcgtttgc gcgattgcat tggcactagt
     6061 taagtctttg gctaaaagga tcgttcctag caggttttga accacagttt ggatttgatc
     6121 gggataactg acttgttcac tggcaatccc tataaaccct tctacagctg ctgcttgtgt
     6181 caatatatga gccggtaatt gtcttggttt gattgtcgtc agtggaagga atgttgctct
     6241 tcctcctctt tgctgtttca aatacgtgat ggcttgccga gcatcttttt cattttcaac
     6301 gatgacatgc tgggctgccc cacctaaagc tgtttcgata gctaacgtga aatctgcagg
     6361 aacatcgatc agttcagcca ctgctccgac aatacccgaa agctgctgtt tatgctgcaa
     6421 gaccaatcga accccttggt aaaaaccaaa atagttttct tggatttctt gaaggctttt
     6481 cttacgagca cgtagctgct ggacttgatt cattaattga tacattttag gctgttcttt
     6541 tgctaatgct tcttggaact ttttcccgtt ttgttggatc atttctaact tctcttttgt
     6601 ttcagttaaa gatgatttta gctgcgcctg cttttgtgtc aattcttctt tttgtaagat
     6661 caatcggtct actgatgcct ctacttccga ttgcttggct aacgtctgct tactttttgc
     6721 tgtttcttgt acatattgac gttctagata cttcagttcg tttcctacag ctgcttgctc
     6781 ttgcatcaaa tcaacatatt gatctcttag ttctgccagt aattctttcg ttgatttttg
     6841 gtatttttct aattcttgct gtgtttttat gagacttgct tcagcttttt ggatttctgt
     6901 ttctttttct gctacttctt tcaacaagct ttcttgaagc ttttcaaaat gtttaacctt
     6961 tttttgtgct tctgctaatg aagtttgata ttcttgcgag cttttttgtg tatgctttgt
     7021 ccgctcctgc aaaacatctt tttgtccttc tgtctgcttc agtttttccg aaagatctaa
     7081 taatacttgc tgattttttt cgatcagacg atcggcttgc gcattttctt tacgctgctt
     7141 cgccaaaatg gattcttgtt cttggatcga ctcagatagt ttgcctaatt ctagattgaa
     7201 tttcgcaagc tgtgcttgtt tgttgtccca atcttttttt gctgtcttga tttcggcaac
     7261 catcaaagag acatcggttt gagtcagtgt ttcttttagc cgtaaaaact cctttgctgc
     7321 ctcactctgg gcggccaaag gagttaactg ttcttccaat tcgtggatga tgtcttggac
     7381 gcggctgagg ttgtcttctg tttcaaaaag cttctgttct gctttctttt tccgctgctt
     7441 gtatttcaag acaccagctg cttcttcaaa gatcccacga cggtcttccg gtttactact
     7501 gaaaatcgct tctacttttc cttgagaaat gattgagaac gattcttttc ctaatccaga
     7561 gtctaagaac aattcttgga tatcttttaa tctgcagctt tgtttattga tgaaaaactc
     7621 gctttcccca gtccgtcgat aacggcgggt tacactgatc tcattgtact ccaatggcag
     7681 ataatgatca ctattgtcta aaatcactgt gacttctgca atattcagct gcttacgggt
     7741 atcggaaccg gcaaagatga tatccggcat ttttccgcct cgcagacttt tagcagattg
     7801 ttctcctaat acccagcgga tagcttctgt aatattgctt tttccactgc catttggtcc
     7861 aaccactgct gtgacgctat tttcaaagtc aatgactgtc ttgtctgcaa atgatttaaa
     7921 accggctatt tcaattcgtt tcaaatacaa agctttccct cacttacttt tacagtgctg
     7981 ccaatgcatt ttccgccgca gcttgttctg ctaatttttt cgattttcct tgtccagtgc
     8041 caatcaattg atcatctaca taaacttcga tccaaaatac tcgttcatga gctggacctt
     8101 cttcattgat caatcggtat tcgattgata catcacctga tttttgcaag acttcttgta
     8161 gttttgtttt atgatccatc tcatgtgaaa aagcacctgc tttgacttta ggaaaaatca
     8221 ctttttcgat gaatgcatgg gtcgtgtcga atccctggtc aagatataac gcgcctaaaa
     8281 aagcttcgaa taggtcgcaa agcaatgctg gtcgcgtacg tcctcctgaa ttctcttctc
     8341 ccttgcccaa caaaacatat tgatcaaagt gacattcttt ggcaaatttc gacaagctgt
     8401 cttcgcgaac gatcgcagct cgtgtttttg tcaattttcc ttctggcaaa tgaggatata
     8461 aacggaataa atactgagaa accatcagtt ctaaaacggc atctcctaaa aattcaagtc
     8521 gctcattatc cgataattgc agattgcgat gctcattcac ataggatgaa tgggtaaaag
     8581 cttgttcaag caaatttaga tcgtgaaaaa cgatgttata gttttctttt agttcttttg
     8641 tcagctggtt gtccattttc cacattcctt ttatggatat tttttcgata cttccttatt
     8701 atactggttt tttgtcaaat ttgaaatggt tcggtcaaaa ggtaagtaaa gctttaataa
     8761 tcctagtgat tgacgagaag ttgtgaaaaa ttcgtttagt ttcgagtatg aagaaaaaat
     8821 tttgtgaaaa tggctttcca tatttttgca taaatttgac ttaataccga gaaactggac
     8881 tttgaacacc gtttattgga gaagtcatga aatcagcgcc ttgacctgaa tattagagaa
     8941 ataaacagaa aaatatcaaa aaaccgaaaa aggacatcgc cctttttcgg tcatttttag
     9001 tgattatgaa cgtgaatctg ctgtaacaga aacatttgcc catgggtttg gtacatcata
     9061 agaccaatcc cagtctttga cacggtcgtt gactggcaat acttcattac ggaacaacgt
     9121 tggaatcgca aatgcttctt caaatgcata ttgttgccat ttttgatatg cttctttttg
     9181 gtactcttca tcaaatgctt tgcttgaact gatatcgttg atcaattttg tattttcttc
     9241 tgattcataa cgagtgtagt tgaacgcagc atttggtccc catagtccac ttggatttgg
     9301 atcagttcct gtattccaag cagcttggta aacatcaatt tcaggatcat cattttctaa
     9361 tttatcatag aaagaattga attcgatcag acgacctgtt gtgtattgga catccaaacc
     9421 gatttctttc cactgttgga tatagtaatc tgccaatggt tgcgctgttt ccccaccgga
     9481 catcgaagca aagttgattg ttagtttatt tccatctttg tcttctctta aaccatcgtc
     9541 atccacatct ttgtagccag catcatctaa tagttttttc gctttgtcta gatcttgtgt
     9601 gaatcctttc atctcatcat catggtatga ctggaatact ggaggaatca atgaagtcgc
     9661 attgcttctc aaacctgcat agaaacgctc acctactgca ttattatcta atgcatagcc
     9721 cattgcttga cgtaatgatt tatcagccat ttttgaatct ggattgtatt ccacactctt
     9781 tgtatctgca ttccatttac ctaacttgaa tcctagataa gtataagaag tttgttcacg
     9841 gcctaagttt gtatagccag caatttcttt ataggtatcg taagtatctg ttggcatttg
     9901 gaaaatcata tcatataatt tagagttcaa tgcttcagtt gcacttgcca tcggtacaga
     9961 tttcatcgtg atctttttca atttaggcgt accattccag tagtgctcgt ttggcaagta
    10021 ttctactgat tctcctgcaa cgatattact catgtaataa gggccaaaag tcactggatt
    10081 tttacgtact tgatcgcttg attccatatc tttgattgct acaccttcta acgtatgttt
    10141 tggtgctgca tatgaccaaa taccaccacc cgattgaagc atgcttgggt ttacttcttt
    10201 atattcgatt tcaactgttt tgtcgtctat ttttttgatt ccggagattg tgtcggcact
    10261 tccatcatga tattcttcca tacctacgat gttacggaaa gtgtcgtcat aacggatacc
    10321 agtgtaatct ttattcccaa tgatttcgta agggaaaatc acatcgtccg ccgttacatc
    10381 ttcaccatct gaccatttga cgttgtcttt tactttgatt gtcgcttttt tcgtatcttc
    10441 atctagatca agcgttgcag ctccgtcgtc tgtgattctg aaatcgtcat cgtttgcaaa
    10501 tagcggttca tgagaaggag ccataaaagc tgcatcatat gaatcttggt aaaactccca
    10561 ctggaacaat cctttaaact gactgtccgt tgcgaccgca gcttctaatt cgccgtcttc
    10621 gattgcatca tcattatttt ttgttgcaat tggaaaagtt aattcctctt tgttattgcc
    10681 gttgcctcca gagttgtttc cagaggtatc tgtacctccg cccccgcatg ctgcaagaac
    10741 gacagcagac atcagcgctg caaatccaaa aatcgttttc ttcattccta tttccccctt
    10801 cgcttctata ctagcctaac cgttggcgcg catctgcaga acgcttgaat gcttgtccaa
    10861 cataattgat actcaacatc aaaactaaaa taaggattga tgcgggtaac cagatccatt
    10921 gccgattcac taagacttct ccgttacttg cataaccgat caatgtacct aaactaggta
    10981 catttgccgg caagccgaaa cctaagaaag tcaaagttgt ctcgataccg atgtttgccg
    11041 caaagttcat cgtcaaatta gtaatgatta atgaacttag attcggcatg atttcgcgga
    11101 acatgatttt aaaatcattg gttcccattg tttttgaggc actgacataa tctcttcttg
    11161 cttcagataa tgtcttgctt cggaataatc gggctttagc tacccaataa aatgcactca
    11221 tgatgaatat aaaggaccag acattgtatc tggaaataat cgatacgaag acgataatga
    11281 tcagcatgat cggtagaatc atgataaaat caacgatccg catcaagatg ttgtcaatga
    11341 tccctccgta gtagccagag atgatcccta atccaacccc gatgatcgaa gtaaggatcg
    11401 tgatcgcaaa tccgatcgta acagagtttc tagcacctat gatcaattgt cctaatacat
    11461 cacgtccgcc ttcatctgct cccagaatga acttttgtcc ttgtgtcgaa actgttcctg
    11521 gtgcagcgta tttgtcgaag atactaacat acatcacttt gtcttgatcg gtcagcatcg
    11581 caccgataaa gatcactaat aataaaacca ctagcaaacc taatgaaaaa atagcaattt
    11641 tatctttttt aaattctcga gcaatcattc gtatccccat aggaggaata ctttcttgaa
    11701 ctgctgtatt gtttttttca ctcatcgatc cattctcctt tctgattggt tttattgaat
    11761 ccgaatacgc gggtcaacga tactcatgat gatatctgaa agtaatgtcc ccactagcgt
    11821 cgcgattcct aaaatcaata ctaaggatgt gatcacggta tagtcccgtt ggccgatgga
    11881 atcaataaat agttttccga ctcccggata accaaaaatc ttttcgataa ctacagaacc
    11941 agcaatcaac gaagtgattt cataccccat ttgagaagcc atcggcaacg ctgcatttcg
    12001 gaaaatatgt cgactgaata ctttattcgt cggtacccct ttggaacggg ctgttctgac
    12061 aaaatcgagc gatttcgaat cgattacttc acttcgcaga tattggattg tgacggcagt
    12121 cgccaaaaat gcttgggtaa ttgatggtaa cagaatatat ttcaaacggt ctatccattg
    12181 agtgataaat ccaccttgca aacctagtgt ttgtgatcca cttgttggga accagcctaa
    12241 acgataacca aaaacaaata gcatgatcaa gccgaagata aataaaggaa cagcaaaact
    12301 aaagaagttg tagattacga caatcttatc caagattgaa ttttgatacc ggccagcaag
    12361 catccccaga ggcacagcaa tcaaataagt aaggacaact gtcaagatcg atagatataa
    12421 agtgttgatg atccgttcgc cgatcaagtc agcaacagag cgtttgtata agaaactttg
    12481 accaaaatcg ccttgcaaag catttccgat ccaacggaag tactggacat accatgggtc
    12541 attcaagcca gctgcttgtc gcatctgttc gatcactgcc ggatcctgat ttggattgat
    12601 caaaccggta aatggatccc ccggcatcgc ttgggctaat aagaagatca gaacacttaa
    12661 aataataact tgcgggatca tcaataaaac acgtcgcaga atcgttttcc acattagctt
    12721 gcacctccat ctttcaacgc cactttgtgt gtagttgtga tcgtacgcaa atcgtagact
    12781 cgaccagtcg catcatagta ttctttttgg ttttggatat actcttgttc tactcttcgt
    12841 cgattttctt tgtggctttc cctattttct acatcgattt gcggaatagc tgaaagcaga
    12901 cgtttcgtat agatatgtcg cgggtcatta taaatgtctt ctcttgtacc gatttctaca
    12961 aatcgacctt tgtacataat tgcgatattg tcgcacatgt gttttactac acctaaatca
    13021 tgtgaaatga ataaatagct gaggccgtat tcttgctgga ttcgtttcat gaaattcaat
    13081 acttgcgctt gaacagatag atccagagca gatacaggtt catcggctac gatcagtttt
    13141 ggattggtag caacagcacg ggctactcct agacgttgac gttgtccgcc tgaaaattca
    13201 tgaggatatt tatatagcgc atcttccggc atcccaacga tatctagcag tccttttacc
    13261 tttttctttt cttcttgatc gcttaaccgt tcaaaatttc ggatgggttc agcaatgata
    13321 tccaacactc gctttttagg attcaaactg gacattgaat cttggaagat catctgcaca
    13381 tccttgttat aacccatttt tttgcggttg cttcgcttcg ttacatcttt tccttcatac
    13441 atgatttgtc cggctgtcac cttttctaat cccacaacgg cttttcccgt agtagatttt
    13501 ccagagccag attctcccac taaaccatat gtctttcctt tttcgataat aaaatccaca
    13561 ccatctacag catatacatg atccgttact cgattgaaga atccactgcg aatagggtaa
    13621 tgaactttca ggtctttgat tgtgattaat tctgtcatct acacctctcc tttttcgtct
    13681 ctaaagtgga aatgcttata gcaggagcaa cgtaccaagt ggttcggtgc gacttcatga
    13741 aggataggct cttcttcatg atcgcttgct ggtatccatg gaatacgtgg tgcaaaccga
    13801 caacctttac gaggcatgtt tttcagagaa ggaacgacac cttcgatcac atggagttct
    13861 gcatcatgat ttccttcttg tggtatagag ttcagtaaag agcgtgtata aggatgctta
    13921 ggatgatcaa acagttcttt tacacctgct acttcaacga actgtccgcc atacatcact
    13981 gccactcgat cagccatttc tgctacgact cctaagtcat gagtgatcaa gatgatccct
    14041 gtttttgttt cttcctgaag gtctttcagt aaatcaagaa tctgtgcttg gatggttaca
    14101 tctagggctg tcgtcggttc atctgcaatg acgattggag gtttgcaggc gattgcgatt
    14161 gcgatgatga cacgttgtct cataccgcct gatagttcat ggggatattg gcgtccaact
    14221 ctcgcaggat taggaatccc tacttgttct agcaattcta gtgcacgagc ttgacgctgt
    14281 tctgcattca tctttgtgtg ataggaaagt ccttctttga tctggtcttc gattctcatg
    14341 agaggattta atgcagacaa cggatcttgg aaaatcattc cgatatcatt tccgcggatt
    14401 ttattataga gcgtttcgtt caggtcgatt aaattcagat cgttatataa aatctcacct
    14461 gtaatttttg tgttattagg atcaagtaat cccataatcg tagtagccaa ggtacttttc
    14521 ccgcacccag attccccaac gattgctaaa atctcgtttt tgtctaaagt aaatgaaacg
    14581 tcatctactg catcgtaata gtcatctttg agtcggaatc ctgtatgtag gttctgaact
    14641 tctaataatt ggttatcgga caatcgaata cccctctctg cttcctaaat ggaagttttt
    14701 cttaataaaa aaggacatta cacagaataa agcgttttat aacaaaggtt attcttatct
    14761 ttgtgtaatg aattattaat aattatatag aaaatcgctt tactttgcaa cataatttct
    14821 gtttatcgcc agaattatca gaaaaatgac ttttacgtgt caaaaagatt aaaaaaagaa
    14881 ggataaaaag ctgtatgcag ctttttatcc ttctttgctt ttatttgaaa ttatttaaga
    14941 tggctgctga tataatctac tgcgccaccg actgtttcaa tttgttcagc gtcttcatct
    15001 gaaatttccg taccgaattc atcttcaagc tctaaaacga attccataat actaattgaa
    15061 tcagctttta aatcatcttt gatgttcaat tcatccgtca ctttatcggt atctatctca
    15121 aaatgatttg aaataatttt cgctactttg ttaaatactt cttcgcgagt caactttttc
    15181 acctccaaga atcatacact tgtcaattac ctacaccatg gctaatttta ctggcatttt
    15241 tcaggaatgt ccaccttttt ttcaatggaa cggattgctt cagatatcca aatactattc
    15301 ttcgcttttt tcaaattgtt cgactaattg tccaactaca tctgtctcaa gcattgtgtg
    15361 gatttggcgg atcgtatagc gaacagcatc ggggcctgtt gctccatgag ttttaatgac
    15421 tggtgctttc aagccaaaca aaacggcacc gccatgtttg gaataatcca tttctgcttt
    15481 caaagaacgt aagccgtctt ttaaaagcat agcgcccatt tttcctttga tcccttcatt
    15541 tagaatagca gattttaata gattcatcat attcattgct gttccttcaa tagatttcaa
    15601 aacagcgttg cctgtaaatc catctgtgac cactacatca gccacaccat tgagtagctc
    15661 acgagcttct acgtttccaa taaaattcaa gcttgtttca ttctgaagga gttcataggc
    15721 ttttttcgtc acttcactac cttttgttgc ttctgttccg ttgtttaaga gtgcgacacg
    15781 aggacgtccg atacctcgga ctttacgtgc ataaaaagaa cctaaaatac cgtattgcaa
    15841 caaatgttct ggtttgtttt ctgcgttagc gccaagatcc agcatatcga aaccggcacc
    15901 ttctttccct actactggca aggtagacat caagccaggg cgttcgatat ttttgatacg
    15961 tcctacgatg aacaaccctg ctgctagtag cgcgcctgta ttccctgctg aaaatacagc
    16021 atctgcttca ccgtttttca ctgcttgtgc agcaaggacc attgatgcag tcttttttcg
    16081 tcgaattgct tttactggct catcatcact attgattttt tcgtctgtat ggataatcgt
    16141 gatgtttttt tcatttgtta agtatttctt gatttctgct tcttttccgt aaagtaagaa
    16201 ttctacttct ggaaaatctt gctgcgctaa agccacgcct tctacaattg cttgaggtgc
    16261 gtgatctccg cccattgcat ctacggcaat tttcattgtt tccactcctt tttccgtcat
    16321 cagtataaag tatagcacag tctgctaatc gaaataacga ttatcctgat tttctatttg
    16381 gaggctttgc gctaaaggct gatactcagg aagcatctgc caatccttta tctgccaaag
    16441 ggctgcagcc tcttctctgg ctacctccaa aacatttcca tccgtcacca agtcagcaat
    16501 cgcaaattga ggcagaccag attgacggaa gccaaatact tcaccaggac cacgtaattc
    16561 cagatctttt tcgcttagta caaaaccatt ggtggtctct gtcatgattt tcatccgttc
    16621 cacacctagt tcatttttag gattagcaac taagatacag taggaagcat cacttccgcg
    16681 tccaacacgt ccacgaagtt gatgcagctg tgccaatcca aatcgatccg catccatgat
    16741 cagcatgacg gtcgcgttcg gcacgtttac ccctacttca attacagtag tcgaaactaa
    16801 tatctgcatc tggttgtctt tgaaggcttc catgattact tctttttcct gattcttcat
    16861 tttaccatgt aacaagccaa cttcatattt tggtgcaaat agttcacgaa gtttttcata
    16921 gatttccact gcatttttta catctaatgc ttctgattct tcaatcagcg gacagatgac
    16981 atacatttga tgtcctcttg ccagttcttt tttcgtccaa tccagtacac tgtccaattg
    17041 aggtgttctt acccagcgtg tttcgatcgg gatacgtcca gctggaagtt catcaatgat
    17101 agatacatcc atctcaccat atgctgtaat agctaatgtt ctaggaatcg gagtcgctgt
    17161 cataaataag acatctggtc gccagccttt ttcacgaagt actttccgct ggttcacgcc
    17221 aaaacggtgc tgctcatccg taataactag acctaaattc tgaaattcga ctccatcttg
    17281 gatcaatgca tgtgtgccga tcacgatgtt aatttcccct tgctctagtt tttcaaggag
    17341 ctcacgacgt tcttttgctt tcgttgaccc cgtaagcaat gcgatggtta cttcattggg
    17401 atcaaacagc tgttgcaagc tttccatatg ttgttgtgcc aagatttctg taggcaccat
    17461 caacgcacct tggaaacctg ctgtaacggt agcgtataac gcaatcgcgg caaccactgt
    17521 ttttccgcta cctacatctc cctgcaaaag tcgctgcata tgctgcggac tgcgaagatc
    17581 ccgacagatt tcattcgtta cacgtttttg tgcatttgtc agttcaaaag gtagcttttg
    17641 agtaaattct ttcaattttt ggacatcata aaggatctcc aaaccattcg tttctgcttt
    17701 ttctgctttt ttcaatcctt gaagacgcat ttgaaataaa aagaactctt caaaaacgac
    17761 acgtcgtttg gcttgatggt tttcttcgtg gtcttttgga aaatgcatac ttcttacagc
    17821 agaagctcgg tcaagcagtc gatatttctc cacaaggttg tttggcagat tttcttcaat
    17881 caatgtgccg tattcttgaa accctttttt gattagatca actaatgtcg tttgacgaat
    17941 tgatttgttg acgtgatata tcggcgaaaa gtcatccatt gtttgagaac ctaaaatctt
    18001 catcccgttc aatgctttac gctttgcatc ccatttaccg tatacggcga tttcctccga
    18061 taaaatgact ttttctttta gataaggttg attaaaaaaa gaaacgttga acacatcatg
    18121 ttcttgcatc atacgaaatt gcaatcggct ttttttataa ccaaatcgat tcatcacagg
    18181 aggcgagact acgattcctt tcaaagtaac cttttcttga tcttggatct cgtttagatt
    18241 tctttcctga atatcttcgt agcggaaagg ataataagtc agtaagtctt caatcgtttg
    18301 gatgcccaag ctggcaagac tagctgctct tttttctccg actcctgcca gcatagtgat
    18361 tggatcatct aaagaacgca taaatttttc ctccctccct taacaaaaaa aggacttggt
    18421 cagcgatcat acctgatcac taaccgaagg ccttttatga atgaatactg tgttagaaag
    18481 atattctgac acagaaatta ttcagcagaa aatagataag gatagacagg ctgatctcct
    18541 tggtggatct cgatttccaa atcaggatgt ttttctgtta gtttctcagc aaattcattt
    18601 gcttgttcaa ctgttccttc atcacctgcc aagatcgtta cgatctctgt gtcctcattg
    18661 atcatttgtt ctagtgtact taacgctgtt gctgtgatat ctgggttaga gatcaggatt
    18721 tttccatcga tcatgcccaa aaagtctcct tctttgatct ctacaccgtc aattgaggta
    18781 tctctgatcg cgtgagtgac ttgaccgctc acaacgctag acaacatttc tgtcatcgtt
    18841 tctttgtttt cttctaaaga agcttgatca ttgaatgaaa gcatagctgt caatccttgg
    18901 gaaatcgttc ttgaaggaac aactgctact gggatatctg ctacttctgc tgcttgatca
    18961 gctgccatga agatgttctt attgtttggc aagacaatca ctttatctgc gtttacttct
    19021 tgaatcgctt taacaatgtc ttccgtactt ggattcattg tttgcccacc actgataaca
    19081 taattagcgc ctaaactttc aaacagctct tggacgcctt taccagcagc gatcgctacg
    19141 ataccatacg ggatacgtgg tgtttctttt ttcgcaaact cgttcacttc ttcatcgtgt
    19201 tccaaaatcg tctcatgctg caagcgcatg ttgtcgactt tgattttgac taatgaaccg
    19261 aatttttgtc cataattcaa gacttctcct gggttttctg tatgcacgtg gactttgatg
    19321 atctcgtcat cattgaccac aagtaaagaa tctcctatac catctaaata gttacggaat
    19381 tcttcgtaat cgaatgtact atcgactgta ggtccttctc cgatttttac catgatttct
    19441 gtacagtaac caaaatggat atcttctgtc gccatttgac cttggacact gcgatgatgt
    19501 tctgcattga ccatatcatc catttctgct ggagaaggtt catatgtttc atccgcttgg
    19561 aattctccat tcaatgcact taaaaagccc tcataaacaa acagcagtcc ttgccctcca
    19621 ctgtctacga caccgacttc ttttaggact ggaagcaagt ctggagtttt agctaaagca
    19681 cgctttgccc cttttacaac agctgtcatt acttcaacga catcatcagt actttttgct
    19741 ttcttttctc cagctttcgc tgcttctcta gctactgtca aaatcgttcc ttctactggt
    19801 ttcattactg ctttatacgc agtttcaact ccatgagtga aggctgcagc aagatcggaa
    19861 gcatttaatg agacaacatc tggaatttgc ttagaaaatc cacgaaacaa ttgagacaaa
    19921 ataacgccgg aatttccgcg cgcacccatc agcaatcctt ttgaaagcat tgctgctaat
    19981 tctcctactt tttctgaggt tgaatctact actgccttag ctccactagt catagataga
    20041 ttcatatttg ttcccgtatc gccatctggc acagggaata catttaatga attgacgtat
    20101 tctgcattta cttgcagacg attcgcacct gcctggacca tttcctggaa ctggcttgca
    20161 cttatttctg ttacactcac ctagataatc ctccttcaga tcgactgctg agttaccagc
    20221 cagtctcctg cctagtctgg cagtacacga acaccttgta caaagacatt aactgaattg
    20281 gcagttacgc ctaataatgc ctctagattg tatttcactt tttcttggac gttgcgggaa
    20341 acttcagaga ttttcgtacc atagctgata atcgtataaa catctacagc aataccatta
    20401 tcttcttggc ggacaactac tcctcttgag taattttctt tacgcaaaat ctcagtaata
    20461 ttatctttaa tttgattttt gctggccatt cctacaacac cgaacacatc tgtcgcggcg
    20521 ccaccaacaa ctgttgcaat tacatcattg gtaatttcaa tggttccagc tgacgtttga
    20581 atttttacag ccatgaatga gcctccttaa cgggtaaaaa actcccattt ttcttctatc
    20641 atattttatc atagcgcaac ttgaaataaa aggcagttac ccaaagtact caaaaacctt
    20701 gcaaaatttc aggtataacc ttgtttaatt tacaattatt cttctcaatt gtcaattgta
    20761 tctatgaaaa tgtacttgca aatttccatt ctatatgata tgttagtctg gtatgagctg
    20821 aattattcta ttgctccaaa tcaaggcaaa ggaggaaatt accatggcaa aagtttgtta
    20881 ctttacaggt cgcaagacaa aaagtggaaa caaccgttca cacgctatga actcaacaaa
    20941 acgtactgtt aaacctaact tacaaaaagt tcgcgttatg gtcgacggaa aacctaaaaa
    21001 agtttgggtg tcaactcgtg ctttgaaatc aggaaaagtt gaacgcgttt aattgaatga
    21061 tacttggacc gttcagatag tgaacggttt tttatttaat aaaaattttt aagggtggga
    21121 cttttgattc ctaccctttt tttgcgttta tttttgttat acgaacaaat tatgaagtta
    21181 tttattctaa attttatttt tttgtgaaaa ttagactctt tttaagcaga gaaaaacaag
    21241 gatatgataa actgaataca acttcataga aatttggtgg aaaataaata tgaaaaaaaa
    21301 tttaaagtca ttctttagag aaaacggacg ctacgcagca gctagtttcc tgattccgtt
    21361 tttgatttta gcacttgttt atctaagtat cgggatctat cctggaagca gcagaagtat
    21421 tttggcaagt gatgcctttt cacagttttc taattttcat gccagcttcc gaaatgttct
    21481 tcttggaaag caaagtattt tttatacatg gaatgcttct ctaggattga attatttatc
    21541 tttaatttct tactatctag gcgggatctt cacaccactg gtgttctttt ttccaaacca
    21601 gatgatgcca gatgcactgt atgttctgac tttgcttaag gtcggctgtg ccggattggc
    21661 tttttggttt cttgcacgta cgttcaagat tcctcgctgg gggcatgttg cacttagcgt
    21721 cagttacgca ttgatctcat ttatcactgc acattcggag atcatcatgt ggctggatgc
    21781 atttgtttat cttccattag tgattttggg gattcatcgt gttatggatc ttaaacggcc
    21841 gaaactttta tttatcagtt atttactctt gtttttatcc agtttttata tgggttttat
    21901 gattggcgta ttttctttct tatattttat cgcccgtcta ttgacaaact ggtcagaata
    21961 taaaaaaacg attgttcctt atggtatcac ttctttactg gcaggcggcg cttcaatgat
    22021 cattgtattg ccagcagtgc tggatcttcg gtcaaatggc gaggcactga gtcagatcac
    22081 tacgttgaaa acagaagcga cttcttatct ggatctaatc atgaaaaata tgattggtgt
    22141 ttatgacacg acaaaatacg gatcgattcc gttcgtttac gcaggtctgc taccattagt
    22201 cctctgtctg ttttattttg ccagtcggaa agtaccgttg aaaaataaat ttttgtacgg
    22261 cagtcttttc gccatactta tcgcaagttt ctatcttgtt ccattgaatt tgttctggca
    22321 tggtatgcat gcaccgaata tgtttttatt ccgttatgcc tatctgttct ctttcttagt
    22381 gctcctgctt gcaggaaaag gatgggaaaa gatagaacaa gacgatcgtg ggctgttgtt
    22441 aggaatcatt ttggtcgtag ctgctctctt tgcggtggca tggggcatca aacctgaagg
    22501 aagctacacc tatgtgacga tgacttcctt tgtattgaca gtggttttct tagggattta
    22561 tgcttttgct gtgggatttt atcagcataa aaaactttca ttcaatcatc tgagtatatt
    22621 gttattattg atcatgtctg ctgaagcact ggtcaatacg aacagtatga tccatggcat
    22681 tcttgatgat tggaattatg cttcccgcag tttatatact gatccttatc ctgacttgaa
    22741 agctcttgta aatgaaacaa aaaaagattc cgactcgttt taccgcttgg aaaatctgga
    22801 tccagtatct gcaaacgatt cgatcaacta tggttacagt ggtatcagtt tgttttcttc
    22861 tataagaaat cgtaattctt cctcttacct ggataaatta ggtttccgtt caagagggac
    22921 aaatttgaat atccgttata acaataatac tttgctaatg gatgggttta caggaatcaa
    22981 atataacatt tctaaaaacg aaagcacgtt cagtaaatat ggcttccgcc aagctggtca
    23041 aagtggcgac tatacactgt atcagaatgt caatgcattg ccactagcat ttactgcacc
    23101 gttgtccgtt aatgatgttg agcagccagt gacagataat ttgaccagtc agacaaactt
    23161 gatcaataca ttgtctggtt taaatgaaca gtattttaca ttctatacac ctactttgaa
    23221 gaatcagttg aatacgacga tcacaaagac aacgactggc gtcacttact cagaaacagc
    23281 agataaccag ccaaaagagc tcacttggga agtccatgtg cctgcaaata cacaagctta
    23341 cttaagtttg ttcccaacca attttgcaca attagaaagt tctagtgcga cgatcactgt
    23401 caatggtaca tcgcgaaaaa cgcaaatcaa catttctggt caatactatg atcttggcta
    23461 ttatcctcaa gagacaacag tgacatttac tgcaagtttt tatggaacga aagaagtaag
    23521 tttcatggag cctaaagtcg ttggtctaga tgttgtagcc tatcaagccg caatgaatca
    23581 gatcaaagaa aaaggtgtcg aaatggaagc cactggcaga aaagcttctg ggactgtaaa
    23641 taccgctgaa gattccatgt tggtgacaac tattccttat gacaaaggat ggaaagctaa
    23701 aattgatggg aaaccagtca aggtcagcaa tttcaaagac gcattcttga tgataaaagt
    23761 tccagcaggt aaacatactg tcactttcag ttacttgcct gaaggattta cgataggtgc
    23821 aatccttttt gtcctatgta ttgcattgtt tattctttat gtctggtacc ttgaccgcaa
    23881 tcgccccttt tatggcacga ttgatccagc aatagaagaa aaaccgcgaa atacacacag
    23941 aagaagacga agaaaataaa gaaacgatgg aatctgtgtc actcttctat ttaaatggca
    24001 aaaatttcaa tctttaaatg acaaaaacca cctgattttt ggtggttttt tatataccta
    24061 tgactaaact atctcttgct gcttcgacaa cttctacagt gatcttatcc atttgattga
    24121 ttcttaaaat ccgatcaatc tgcgcaaata aacgatgaat aagcctaaaa tttccttttg
    24181 tgatcttaat gatagttgtg atagcctcat aatcagaaaa atcttctagc ttgatatcaa
    24241 ttcctaaatc tgcccatttg tattccaaaa tatgatgtgt ttcgtcctta ctaaggttat
    24301 caaattcatg tgcaaatcct atgcgagagt aaagctgcgg ataacgagac aatctttttt
    24361 caataccagg cataccgata agaaccattg ccagatcatt tctatcgtaa atatctctta
    24421 gctgttctaa atgctgaagc tttaaacgat caatttcatc gacaataatt aagtcaattg
    24481 cttcataggt tttaggcaca ggaggatctt cttgattgta ttttagtacc atatgcattg
    24541 ctttagtcag acctattcta ctagttattg tgttaatatc cgaactgaca cgagacatct
    24601 tttcagctgg tacagtataa aaaactttat ttacacttaa tatttcatct gttgcattta
    24661 tcaagaacaa gaaaaagtct gaatattcaa aaaataacgg tgtacatttt taaaaggaga
    24721 tgtataatcg agatttagaa agcgttttcc taaaaattta atgaattagg aagtgattac
    24781 tatgaaaaaa ttatttagcg cactttttat tctgttattt ggctttattg gtattgggac
    24841 ttctggaaat atagttaaag ctagcacgat ggttgaacaa acaagtgaaa tggcaaaaac
    24901 ttttagtcaa ttaacaccag aagaacaaag ttacttcaaa tccaaaggat ttagtgataa
    24961 agatactttt tacactcaaa agatagagcc tcaagatccg aaaacacgtt tttctgcaaa
    25021 tgtattgcgg ttaaccgcat ctactaaaaa aattactaat acgcaggggc ggacagaata
    25081 tattataaca agtcccaatt cggcgatgta tcgtatttct acacgcttga gtttaggtag
    25141 tgcaaaatca tatactcaaa cagtaaatct agctaattct taaggttatg gtggaggaat
    25201 gtattttaca tttactggga aaagacaata ttacagtgta aaattagcta tgcaagttta
    25261 taatacattt ggtgtagggt cagtctcttg cactgcggga ggattgacaa taggaaaatg
    25321 ataatttctt actatgtctt aaatagaagt gatgatgaag gactcttcgt ttttcaatca
    25381 ttacatgagg atacgatgct aaaagagttt tataatcgga ttaagaataa acagatagaa
    25441 acaaatttat attttttaag tgactctatc ttgcctttta ttagcaaccc taaaattctt
    25501 aacttgttgc agacaaaaga tttgaaaagt ttaccgatta ttgtcattga tgaggaaatt
    25561 gtaaaatatg gcgcttatct aagcgtagaa gaattagaaa aattaactaa cattggaatg
    25621 agtattcagg aggataatgg ggtacatgga acatagaatg aatatgcaca gttgagaacg
    25681 gaatttgaca tttatttaga ccaacagggt ataccctgtt ggtctttttg tgcgttcatg
    25741 atatacttgg tgtcattttt atatttaaat ggcacaaata tttctaatta tttgtcattt
    25801 aacgctgaaa atttttgcta tttatctaaa aaagtgacag gatactcatt tggccttcat
    25861 agaattttct aaatatgaat catcaatgaa tttaattaaa aaaataagta gggcaaaata
    25921 taaatgtttt tgccttactt gtttc
//
LOCUS       DAFEPQ010000033        25805 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.033, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000033
VERSION     DAFEPQ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.033"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            4..846
                     /locus_tag="LP543_001529"
     CDS             4..846
                     /locus_tag="LP543_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL2321699.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            846..1793
                     /locus_tag="LP543_001530"
     CDS             846..1793
                     /locus_tag="LP543_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBL2321700.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            1808..2041
                     /locus_tag="LP543_001531"
     CDS             1808..2041
                     /locus_tag="LP543_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HBL2321701.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            2372..2956
                     /locus_tag="LP543_001532"
     CDS             2372..2956
                     /locus_tag="LP543_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL2321702.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            3087..3755
                     /locus_tag="LP543_001533"
     CDS             3087..3755
                     /locus_tag="LP543_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBL2321703.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            3766..5181
                     /locus_tag="LP543_001534"
     CDS             3766..5181
                     /locus_tag="LP543_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL2321704.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            5199..5768
                     /locus_tag="LP543_001535"
     CDS             5199..5768
                     /locus_tag="LP543_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HBL2321705.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            5878..7632
                     /locus_tag="LP543_001536"
     CDS             5878..7632
                     /locus_tag="LP543_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2321706.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            7592..9358
                     /locus_tag="LP543_001537"
     CDS             7592..9358
                     /locus_tag="LP543_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2321707.1"
                     /translation="MQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            9881..10150
                     /gene="rpsN"
                     /locus_tag="LP543_001538"
     CDS             9881..10150
                     /gene="rpsN"
                     /locus_tag="LP543_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBL2321708.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            10165..10344
                     /gene="rpmF"
                     /locus_tag="LP543_001539"
     CDS             10165..10344
                     /gene="rpmF"
                     /locus_tag="LP543_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBL2321709.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            10376..10753
                     /locus_tag="LP543_001540"
     CDS             10376..10753
                     /locus_tag="LP543_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321710.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            10774..10923
                     /gene="rpmG"
                     /locus_tag="LP543_001541"
     CDS             10774..10923
                     /gene="rpmG"
                     /locus_tag="LP543_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL2321711.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            10947..11930
                     /locus_tag="LP543_001542"
     CDS             10947..11930
                     /locus_tag="LP543_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBL2321712.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            11960..12082
                     /locus_tag="LP543_001543"
     CDS             11960..12082
                     /locus_tag="LP543_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBL2321713.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            12100..13623
                     /locus_tag="LP543_001544"
     CDS             12100..13623
                     /locus_tag="LP543_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBL2321714.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            13647..13886
                     /locus_tag="LP543_001545"
     CDS             13647..13886
                     /locus_tag="LP543_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2321715.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(14079..14540)
                     /locus_tag="LP543_001546"
     CDS             complement(14079..14540)
                     /locus_tag="LP543_001546"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL2321716.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(14735..14953)
                     /locus_tag="LP543_001547"
     CDS             complement(14735..14953)
                     /locus_tag="LP543_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2321717.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            15071..16687
                     /locus_tag="LP543_001548"
     CDS             15071..16687
                     /locus_tag="LP543_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBL2321718.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(16928..18268)
                     /locus_tag="LP543_001549"
                     /pseudo
     CDS             complement(16928..18268)
                     /locus_tag="LP543_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(18544..18699)
                     /locus_tag="LP543_001550"
     CDS             complement(18544..18699)
                     /locus_tag="LP543_001550"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBL2321719.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(18891..19085)
                     /locus_tag="LP543_001551"
     CDS             complement(18891..19085)
                     /locus_tag="LP543_001551"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL2321720.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(18995..19327)
                     /locus_tag="LP543_001552"
     CDS             complement(18995..19327)
                     /locus_tag="LP543_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL2321721.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(19841..20032)
                     /locus_tag="LP543_001553"
     CDS             complement(19841..20032)
                     /locus_tag="LP543_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2321722.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(20126..20512)
                     /locus_tag="LP543_001554"
     CDS             complement(20126..20512)
                     /locus_tag="LP543_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL2321723.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            21206..22411
                     /locus_tag="LP543_001555"
     CDS             21206..22411
                     /locus_tag="LP543_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HBL2321724.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            22428..22862
                     /locus_tag="LP543_001556"
     CDS             22428..22862
                     /locus_tag="LP543_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321725.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            23479..>25805
                     /gene="esp"
                     /locus_tag="LP543_001557"
     CDS             23479..>25805
                     /gene="esp"
                     /locus_tag="LP543_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HBL2321726.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         8894 a         3700 c         4827 g         8384 t
ORIGIN      
        1 aatatgcttc aaaattttat taatggtctg tatgactttc actttttaca gaatgctcta
       61 attacatctg tagttatagg gattgtttct ggaacagtag gttgttttat tattttaaga
      121 ggaatgtctc ttatgggtga tgctatttct catgctgttt tgccaggagt agcaatttct
      181 tatattttag gtattaattt ttttattggt gcgattgtat ttggtctatt agcttctatc
      241 attattactt ttataaaaag caatagtgta ataaaaggag atacagcaat tggtattact
      301 ttcagttctt ttttagcatt aggagttatc cttataggag ttgcaaatag ctcaacggat
      361 ttatttcata tcttatttgg aaatatttta gctgtacaag atattgataa gtggattaca
      421 attggcgttt ctgttttagt gttggttaca gttgttctat tttttaaaga attattaatc
      481 acttcttttg atccattaat ggcaaaagca atggggatga atgtcagttt ttatcattat
      541 ctattaatga ttctattaac tttagtagca gtaacagcta tgcaaagtgt gggaactata
      601 ttaattgttg caatgttaat tacaccagct gctacagcat atttatatgc aaacagctta
      661 aaaactatga tatttatttc ggcgacagtc ggagcgtgct catcattgct aggtttgttc
      721 attggttata gttttaacat agcagcagga tcaagtattg ttttaacagc ggcattctta
      781 tttgtaatta gttttctaat ctcaccaaaa caaaaatttt taagaaagaa ggagagaaag
      841 ttatgatgag aaaatggaaa gcagtactgg gaagtctggg gattttgatt gctcttttta
      901 tattcggggc atgttcaaca aatagtaaag acaaagatac agtggcttcg aacgaaaaat
      961 taaaggtagt agttactaat tcgattttag cagatattac tgaaaatata gcaaaagata
     1021 aaattgattt acacagtatc gtacctattg ggaaagatcc ccacgaatat gaacctttgc
     1081 ctgaagatgt tcaaaaaact tcaaaagcag atttgatttt ttataacggt gttaacttgg
     1141 aaactggagg aaatgcttgg tttacaaaat tagtaaaaaa tgcgaacaaa gaggaaaaca
     1201 aagactattt tgcagcaagt gatggcatag atgttattta cttagagggt cagagtgaga
     1261 aagggaagga agatccccat gcttggttaa atttagaaaa cggtattatt tacgctaaaa
     1321 atattgaaaa acagttagcg gaaaaagatc ctgataataa aaaattctat aaagaaaatc
     1381 tagataagta tattgaaaaa ttggattctc tagacaaaga agctaaatct aaatttgctt
     1441 caattccgaa tgataaaaaa atgattgtta caagtgaagg atgctttaaa tatttctcga
     1501 aagcgtataa tgtgccttct gcttacattt gggaaatcaa cactgaagaa gaaggaacac
     1561 cagatcaaat aaaacactta gttgaaaaat tacgcacaac aaaagttccc tccttattcg
     1621 tagaaagcag tgtggacgat agaccaatga aaacagtatc aaaagatacc aatattccta
     1681 tctattcaac gatttttact gattcaattg cagaaaaagg acaagatggt gatagttact
     1741 atgcgatgat gaaatggaac ctggataaaa ttgctgaagg cctttcgaaa taatttggag
     1801 ggtaggtatg gtacaaatta aaacgactac tatactaatt tatttactag caattcagga
     1861 attatcaaaa agaaaaaaga aattaaaaaa taatgattta gcaaaagttc taagtgttag
     1921 tccagcatct gtaagtgaga tgctggctaa acttagatat gaggactatt tagatatagg
     1981 atttcaatta actgataaag ggaaaaaatt tattgacgat tacaaaaaag gttttaagta
     2041 aaagacttta tttttaggga ggataagaaa atggctattg gagtgattat gataggactt
     2101 accctaatag ctggatataa tttttttaaa tctttctcgg attattttag aaaaggtgaa
     2161 tgatttagat gagtctatta attcttgtag gattgctttt tgtatcagtt attttgctct
     2221 ttctcattag tttagctcta aggaatgagt tcagtaatag acgttaacga ttgaaacttc
     2281 aagtcctgtg taagataata tagttaattc atcttattag gaggaagtac ccatagaaac
     2341 cagagattat attacaactc accaaaaatt aatagaaagt ggactaagcc attttgaaaa
     2401 agaaggattt cagcaaacta agataaggca actgtgcttg accgcaggcg taactacggg
     2461 tgctttttat aaacatttta cttctaagga agcgttattt gaagaaatta ttcagccgca
     2521 tattaatgcg cttattaata tgtataatga agagtttacc aaatacctct taaacttaga
     2581 aaatatagat atagagaaaa gttggagaga gaacttcaat gaattagatc catttattaa
     2641 ttatattttt gagaataaac gtatattcga tttggtttta ttccaatcag ctggaaccaa
     2701 atatcaagat attgttgaaa aaattacatc atttgtgaca aaaaagacag tctgttgtct
     2761 agataagtta aagactatcg gaatgatcaa tcaaaattta gtctttaatg aagaagaaat
     2821 ccacttctac atttactcat tctacgctac attctatgat atattgaagc atagctattc
     2881 aaaagaaaag actatgctat taaccagaag attgtatcaa ttttctgttc ctgggtggat
     2941 tgaattactg agttaattaa aaaaaagctt gcattgagaa ttgttctcag ttatgctttt
     3001 tgataagatc gcactgttat ctgaataacg gtgcattttt gtcagcttaa gataacagtg
     3061 cgatcttatt ttttggaggt gaaagagtgt caaaaatcaa tccgttaacc ttgattactt
     3121 taaatatttt ttttccggtc attatttttt taggaaaggg aaatatattt caagtggggt
     3181 gtttcattat tgctacagtt ttagtattga tgtttcgtaa aataaagaca tgtatttatt
     3241 tcatcttgat ttattcagtt ctgtttttag taatgaaaat tattgatgct atcaagatag
     3301 agttttttac tatatttttt gggacatcct tatatattgt atttcgaatg ttgccggtcg
     3361 taatgatctc atggttgttg gttagtgtct atactagcaa tgaattatta tcctctttag
     3421 aaaaaataca tttaccgaaa aaaataatgt taagtattac agtagtgatc agattctttc
     3481 cgacttatcg ttctgaaata agaatgatta aagaatcttt aaaaatgaga aacattcgct
     3541 tgtctttttt tcagcctctg caatatttgg aatttctgtt agttcccgtt cttatacgtg
     3601 caactatgat tgctgaagaa atgaccgcta atgcaataac aaaggggata gaatctcctg
     3661 tgaaaagaag ttcttactat aaaacaaaca tgagcttatt agactatatc atcctgtttt
     3721 ccgttactat ttttttctta agtattttga tttgaggtga aagaattgtt tgaatttaac
     3781 aatgtttctt tttcatataa ggatgttaag gaacaagcgc ttgttgacct taatttttca
     3841 ataaaaaaag gcagtcttac attgattact ggttcttctg ggagtgggaa atccacacta
     3901 ttaaagttaa tgaatggact tattccaaga ctttatgacg gaaaattggt tggggaaatt
     3961 ttatttgaaa ataactcgct acaatcctta acacaagaaa gtatatcaaa aaatattggc
     4021 tacgtaagtc aaaatcctag aggacaattc tttacaacaa atacaacgag tgagttagta
     4081 ttttcgatgg aaaactttgg atttacaaaa gagcatatgg ataaaagaat caatgaatta
     4141 gctgaattat tagatctttc aaatatatta gataaaaaca ttttttcgct atcaagtggt
     4201 gaacggcaga aaatttctat tggatgtagt ttatcgctaa actctaaatt aattatattg
     4261 gatgaaccgt cttcaaattt ggacttcaaa gcgactaaga aacttgctga cttactttgt
     4321 gatttaaaaa ataaaggatt tactattgtt atagctgaac atcgttttta ctatttgaaa
     4381 gaattaattg atcaagttct atttgttaaa aatagaactg taaaaactac aactattcag
     4441 ttgttaaaaa ctgaaaacaa taacgcatta aggaaatttg acatttttaa tagttcgatt
     4501 gaagttccct atatcgaaaa ggtaagtgca gatgtattaa aagttgaaga aattacttat
     4561 gaaaatattc taaaagacat aaattttgat gtcaaagttg gagatgttat tggtttggta
     4621 gggaaaaacg gtgtggggaa aacaacctta ctaagaatac tcatgaagat tattcgccct
     4681 accaaaggaa agataatcga aaataaaaaa aatttgtcat cgccgttttt agtaatgcaa
     4741 gaaatggatt accaattttt cacagaatca gtgaggagcg agttagccct ggggaatgaa
     4801 atagttagta aagatcagca agaaaaaatt ttaaaaaaaa tggaacttta taaaatgaaa
     4861 gatcaattac cgtttgattt atccggtgga gaaaaacaaa gattattggt ttctatagct
     4921 tcattgtcaa aaactaattt atttctattt gatgaaccta caagtgggtt ggattatatc
     4981 aatatggaaa ggattgctga ccttattaaa gacttaaaag aaaagggtgc agtagttata
     5041 gccactcatg atccggaatt tctttataaa acatgcaata gaattattta tttaaaaaat
     5101 ggtggaataa aaaaagatat aaaacttaga gtagaagatt caactttgat agaacagatt
     5161 tttaatgaca taacgaaata gaattgagga gaatatatat gaaaacaaaa gattatattt
     5221 ttattggttt agctacagtt atttccctag taatttactt tttttcaata atggttagta
     5281 gtattggtgg ggcgtttggt cacagcatat ccccagggat ttttggatta ttatctggaa
     5341 tcatatttgt ttatattagc tataactacc ctagaaaagg aatttttact atttacacaa
     5401 ttgttttatt gttatttttt acgttaatgg gcggggcgta cttaccttgg tttatcagtt
     5461 cgattagtat ggcgatctta gctgatatta ttctatctgt atttggatat gatcgagcaa
     5521 ttcctcaagt tgtgtcttgg gttctaatgc aactaggcag tgctgccgga cagtggattc
     5581 ccatatggtt ctttacagat cgttttcgtc aagactggat agatagagga caatctgctg
     5641 caacaatgga tgcaatgatc cactacgcgg tgggtatatg gggaattatt tcagtgttag
     5701 ttgtagcaag tttatctatg attggtgtat tgattggaag aaaagtctta aaaaaataca
     5761 aaaaatagaa cgtagtttac cttctatctg tttgtacatc tctaatttac gctattacgg
     5821 aaagcattaa cagttgtaaa cgaataagag aagaaattga atgcggggga ggaaaaaatg
     5881 aaagtataca aaaaattatt tagttatgtc cctaaaatga aatactacgg atggttagcc
     5941 actttatttt ctgatttttc tgtcgtatta acagtctatg gatattacag tatttacaaa
     6001 tttcttcagt ctctaattat tgtaggagat gaatatttag ctaagagtta tgcggttcaa
     6061 actgttgctt ggttaacttt aggagcttta ttttatattt tttctggctt attttctcat
     6121 atccttgggt ttcgcttaga gactaatttg agaaaaaagg gaattagcgg tttaacagaa
     6181 gcaagttttc gtttttttga tttaaattct tcgggatata ttcgaaagac aattgatgac
     6241 aacgcggcta aaacacatat ggcaattgct cacttgattc cagatagtgc tcaagccata
     6301 gtaacacctg ttttatcagt tgccttaggc tttttcatta actttaaaat tggattggtt
     6361 ctaatcgcca tgttagtaat tggtgtattc ttgttaaaga aaatgacaga gaatacaaat
     6421 tttattcaaa aatatcaaga atcgttagat gtgttaagct ctgaaacggt agaatatatc
     6481 cgcggaattc aagttataaa gatatttggt gctaaagtaa catcatttaa agccttgaat
     6541 aaagcaatta acgattatgc taagtttgca tataagtatt ctttgagtgg gaaaacacct
     6601 tttgttcttt ttcaatggct attttttggc atagtttgca ttttgatagt tcctataacc
     6661 ttttttataa cttctcttac cactcccaag atcttagctg ttgacttgtt gatgttgttt
     6721 tttttaagtg gaattatttt tgtttctttc atgagaataa tgtatgtaag tatgcacttg
     6781 tttgaagcaa gatatgcagt cgaaacacta gactcccttt atttagaaat gaagcaagac
     6841 caactaaaat atgggaacga gacacagatt aaaaattgtg acattgcatt tgaaaatgtc
     6901 agcttttcat acaaagaaga gtgtgtttta gaaaatttca acttagtttt agaacaaaaa
     6961 agaatttatg cattagtagg aaaatcagga agcggaaaga gtacgatagc taaactaatt
     7021 tcaggtttct acaatgtgga taagggacag attaaaattg gtggtaagcc tttaaatcac
     7081 tattcaaaag acacaattat taacacaatt gcttttgtct ttcaagatac gaaactattc
     7141 aatcagagta tttacgataa cgtttctata gcaaatcctc aagcgccagc aagtaaagta
     7201 tttgacgcgc taagattagc cggttgcgaa tcgattttag aaaaatttcc cgaaagagag
     7261 cataccttaa ttggtagtaa aggagtttat ctgtcaggag gagaaaaaca acgtatagct
     7321 attgccagag caattttaaa agatgcaaag attgttatta tggatgaagc cagtgcatct
     7381 attgatccag ataatgagta tgaactacaa aaggcttttc aatatttgat gaaagaaaaa
     7441 acagttatta tgatagctca tcgattatcc agcattaaat ctgtagatga aattcttgtc
     7501 ttgtcagaag gaaaaatagt agaacgtgga aatcataatg aattaatggc taaacatcga
     7561 gtgtataaac aattggtcac taattatgag aatgcaaatg attggaggct tgacaatgaa
     7621 aaatttttat aaaaaaaaat ttgctttaac tgatcagggc gcagaagctt taactaaagc
     7681 ttctatttct agtttttttg tatactgcat taatatggta ccagcattca ttataatgat
     7741 gctgatagac gaattagttt tagaaaatgc aaagccccgt tggctttatt ttgcagtatc
     7801 gtttgttact ttgcttttca tgtattggtt gttagatagg gagtatgaaa acttatataa
     7861 tagtacttat aaagaaagtg cgcatttaag agtgcaaatt gcagacgatt tgtcaaattt
     7921 accattatcc tatttttcaa aacataattt atcagattta tctcaaacta tcatgtctga
     7981 cgttgaaggt attgagcatg cgatgagtca tgcaatacct aaagccggcg gtatggctct
     8041 gtttttccct tttatttcag tgatgctttt ggttggtaat gtcaaaatgg gattagctgt
     8101 tattttgcca acgttattta gttttgtctt aatcttgtta tcaaagaaat cccaaacgaa
     8161 agccaatact aaatattacg atactttgag agaaaactcg gaagaatttc aagaaactat
     8221 tgaattgcag caagagatta atagctttaa tctatctaaa aaagttcaag acagactttt
     8281 caaaaaaatg gaagagagtg aaaggattca tttaaaggta gaattaagta ctttttcagt
     8341 catggcctta tcctctattt tctcatatgt tagtttagca gtagtcattc tagtaggtgt
     8401 tcacttactg ttaacgggtg aagtgactat actctacgtc gttggttact tactagccgc
     8461 aataaaaata aaggattcct tcgattctat gaaagaagca gtgcttgaaa tattttattt
     8521 agctcctaaa atacagcgaa ttagagctat gaaggaaacc agtatccaag agggatcaga
     8581 tagtcctctg aaatcttttg acattgagtt gcgagatgtc tctttctcat atgataacaa
     8641 taccccaatt ttagatcata tctcttttac ggctaaacaa ggagaagtga ctgcgttagt
     8701 aggtgctagt ggttccggga aaactagtat cttgaaatta gtgtctagac tgtatgatta
     8761 tgatgaaggc tgtattttaa ttgatgggta tgatattaaa cgtgtatctc cagcttcttt
     8821 gtttagcaaa atcgctattg tttttcaaga ggtaactttg tttaatacaa gtattttgga
     8881 aaatatacgt attggaaata gtcaagcgtc agatgaggaa gtgaaaaaag cagctcgttt
     8941 agctaactgc gaagacttta ttgaaaaact acctgacggt tatcatacat tggtcggaga
     9001 aaacggaagt tcactttctg gaggagagcg acaacgccta tccatagcac gggctttttt
     9061 gaaaaatgcg ccaattttaa ttttagatga aattactgcc agtttagatg cggaaaatga
     9121 aaaacgtatt caggaaagtt taaatcgttt aatacaagat aagacagtac taattatatc
     9181 gcatcgcttg aagtctattg aaaaggtaaa taaaattgta gtgatggatc aaggaaaact
     9241 agttgatcaa ggtactcata atgagttgta tcgaaggtca gaaatataca aaaatctcat
     9301 aaagaaaact aaactatctg agaaattcgt ctatgaaaaa gaagctcaat cacgatgatg
     9361 aaataggctt taaattaacc tacaattttt gggatatagt tataattctt tttcaaaaag
     9421 aataatttta ttaaacagca tctaattttg aatacacgaa agaatcagta tgattgaatt
     9481 agttcactta atgtctattc ttataatata aggaataatc aaagttttaa aaaattggcg
     9541 ataaaccagt taaacataaa agattgaata aaaagttata gtttctttca tattgccgtt
     9601 ttaagtagag gaaaagctac ttaaaacggt atttgtgttt gattggacta ataaaagtga
     9661 attcttattt ttcagagcct aaactgcata ttcttttaaa tgatttgatt atctctatgt
     9721 accgataaat tcgttttcaa atagttatct attcatatga gttttatttc atgttattta
     9781 ttgttgacaa gagcaactct tgttggtaaa gtatcagtgt caaaacgtaa cgattacgat
     9841 ttgaggtgtc taaatcaaaa taaaaggagt ataaaggtat atggcaaaaa agtcgaaaat
     9901 tgctaaagca aagaaacaaa tggcgatgat tgaaaagtat gcagataaac gtcaggaatt
     9961 aaaagccgca ggagatcgca ccgctctcgc taaattgcca agagattcaa atccgaatcg
    10021 tctgaggctt cgtgatcaga cggatggtcg tccaagaggg tatatgcgaa agttcggtat
    10081 gtcccgtatt aagtttagag aacttgccca tcaagggttg attcctggag taaaaaaagc
    10141 aagttggtaa aaggaggaaa tatcatggca gtaccagcaa gaaaaacatc taaggcgaag
    10201 aaacggttaa gaagaactca tcaaactgta gtaaaaccag aaatttcatt tgacgaagcg
    10261 aatggtgatt atagacgtag ccatcatgta tctttaaaag ggtactataa tggaaagaaa
    10321 gtaattaaag gagcttcaaa atagtttaat atataacttt taggagggaa tcaaaatgga
    10381 aattatttac ccacctttag ttgaagaagg attaaagtat tatcttgaaa cgacccagca
    10441 atcgctagat aaatgcactt tttatcgttc gatggtagag agaggaatta ttactgaaac
    10501 gggtttaccg acccaacagg caattgaaaa tggattagtc aaagattact atgaggacca
    10561 aggcttatca tttgatgatt ttttaagaat ctatccaatt tttgaagagt atgacgagga
    10621 gttatttcaa tgtatcgatg gttattggga gatacctgtt gatatgaaag aaaatttagt
    10681 ttcacaattg gaatctggcg agttaacttt tgaagatgca caacaaattc aagcatatct
    10741 tgaagatcga taagtattga aggaggaaga tttatgcgtc aaaatattat tttggaatgt
    10801 gtcgaaactg gagaacgtct gtatctcacc agcaaaaata aacggaataa ccctgaacgg
    10861 ttagaattaa aaaagtattc gccaaaattg cgcagacggg caatttttaa agaagtgaaa
    10921 tagctaagtg gtaaaaaggt gacaaaatgg gcataccagt tacaatcgtt tctgggtttt
    10981 taggggctgg aaaaacaacg ctaatcaatc aagccttgca agtctcaccc taccccaggg
    11041 aagaaattat tataattgaa aatgagtttg gtgaagttgg gattgatcac aaattattag
    11101 ttcatagcga ggaacgggtg ttacaactaa ataatggatg tatgtgttgc agtcttcgca
    11161 gtgatttatt agcaatattg atttctttac tggaaacaat agaagaacat caacaaccaa
    11221 tttcacaaat catcattgaa actacaggta tagcagatcc ccaaccaatc attcaaacac
    11281 ttttaacagc gcctcaacta agaggaagag tatatatcga tagtttattg actgttattg
    11341 atagtgacag ttttgaactt attgaaagag aaccacaagc ccttaagcaa cttgtcatgg
    11401 cagatcgcat ttttgtgtct agaaaacaaa acttgaattt atctgcacta aaaaaattac
    11461 agagagaaat caaaagtatt aaccctttat ccgatattcg aatgttcaca catagtgaaa
    11521 cgattcaaaa aaaggatttt tataatttga aaaaatttaa tcatccctta gtttttgaaa
    11581 gtgattcatc agaaaatcac tataatggca aatactatca tcaacaccat caagtacatg
    11641 agtttaaatc aattctctta acttcagatc aagacataaa aaaagatttt ttactccaat
    11701 ggattgactg gctgatttta accaaccaag ggagcctgta tcgttttaaa ggattgattg
    11761 ctttacaaga gcaggatttt atagttgcca tgcaagaggt aaacgaacag gttaattttc
    11821 aatacaccac tcgacaaaaa gaccaaacca ctattattct aatcggaaaa atgttggatg
    11881 aagaaaggat caaacagagt tttgataaat tactggcgga aagcaaataa tgataagtag
    11941 tgattggggg taatttctaa tggagaaaac ggatatatct agtgcatatc gacgacttaa
    12001 aagcccgaat attaaaacta gaaaaagggc attaaaaatt atcaaagaac ataaacgaaa
    12061 taaaatgaag aaattagcat aattaaggaa ggaaaaagaa tgaagaaaat cagtgtagga
    12121 cttattggga tagcagctct aggattattg ggagcttgct ccagcactaa tgatgcaaaa
    12181 gtatcgaatg ataaagatgg aaaattagaa attgtaacaa cattctatcc aatgtatgat
    12241 ttcaccaaaa acatcgtcgg cgatgaagcg aacgtggatt taatggttcc agcgggatca
    12301 gaacctcatg actatgagcc ctcagctaag gatatggcaa aagctcatga tgctgatgtt
    12361 tttgtatatc ataacgaaaa catggaatca tgggtaccaa aagctaaaga aagttggaag
    12421 aaagctgggc ctaatgtagt agaaggaaca aaagacatga ttttattgcc aggtagtgaa
    12481 gaagaagatc atgatcacgg agaagaagat caccatcatg aactagatcc tcatacgtgg
    12541 gtttcaccca aaatggcaat taaggaagtt tcaaatatca aggatcagtt agttaaattg
    12601 tatccgaaaa aagcaaaagt atttgagaca aatgctgaaa agtatttgac taaactgaaa
    12661 cggttggatg ctgactatac cactagtctt aaagaagcga aacaaaaaag ttttgtgacg
    12721 caacacgcag cctttggata tttagctttg gattatggat tgaaacaagt tcctattgct
    12781 ggactaagtc ctgaagaaga gccatcttct ggtcgattag ctgaattaaa agagtatgtt
    12841 aaaaaaaata agatcaatta tatttacttt gaaaagaatg ctaatgataa aattgcaaaa
    12901 actttggcca acgaagctgg gataaaattg gaagtactga atcctcttga aagtcttaca
    12961 aaagaacaaa tggataatgg tgaagattat gtttcagtaa tggaagacaa tctaaaagcg
    13021 ttagaaaaaa caactatggt tgctggtgaa gaggtagttc cagaaaaaga agcgaaggat
    13081 gaaaagacag ttgctaatgg gtactttaaa gatgccgatg tgaaggatcg cgaattgagt
    13141 gattatacag gcgaatggca atctgtttat ccgttgttaa aagatggaat tttggatgaa
    13201 gttttcgatt acaaagcaaa aatcaataaa gacatgacag ctgccgagta caaagattat
    13261 tatactactg gctataagac agatattgat actatcaaca ttaaagacaa caccattgat
    13321 tttgtagtca acggtgaaca tcatcaatac acatacaaat atgttggata caaaattttg
    13381 aattatgaaa aaggaaaccg tggtgttcgt tttaattttg aaactgatga tgcaggtgct
    13441 ggtcgtttta aatatattca atttagtgat catggaattg caccaagtaa ggcagagcat
    13501 ttccatattt tctttggtgg agaaagtcaa gaaaaactct ataatgaatt gcataattgg
    13561 cctactttct atccagcttc attgtctaaa catgaaattg ctcaagaaat gatggctcat
    13621 taaaattcaa tttttggagg aaatacatgg taagaaaagt attattgtct atggattcca
    13681 agaatattga tgcttatcgg cgttatcgat ttgcaaagca aatgagatta aatgggagta
    13741 cattagctga tattgctgca actttacatg ttagtattga cacagttcat agagtattaa
    13801 aacgggatcc tgattgttgt tttatcaagg tcaatactgg aagaagtaga cgtttagcaa
    13861 aacggcataa tagcttggcc aattaattga taataccaga gtagatcatt tttcccttat
    13921 ttggatttga tctgcttttt attatgtctt gaattgtcaa atcaagcgca acactttatc
    13981 aaagaagact tcataaggtg ttctccaatc taaacacttt ctaggtctgt tattcagttt
    14041 ttcaatcatc tgatttatcg ttgagttatc taccgtagct atctctttcg attttggcaa
    14101 gtattctcga atgagtctat tcgtatttta atttgttcat tgttgccaag ggcatgagga
    14161 tctggaaaat aaaagggcat attattcaat gcttctgtaa ctgcgaaatg attggagaat
    14221 tcttttcctc tatctggagt gatcgttttg accgcctcat ttggaatact gcttaaaagt
    14281 tcaatcattt tctcgctcac ataagcggct ttctttctgg ggaccttctt tcctgctaaa
    14341 aggaagcatg attttctgtc tgtcaaagtc actaaacaag taccaccagt tttgccagct
    14401 acaatatcgg ctttccaatg accaaaatca cttctatcat ctgcggattt tggacggcca
    14461 tggatcgaat gagtaaggca aatctttcca cgagactcga catgattttt tgtatgtctt
    14521 gtttttcctc gatggtgcaa tgaacggatc aggcctttat tgccgtgtga gaggggttct
    14581 gtatcgaaaa gatcattgca aattgcacgt tagatcgtga taaaactaat tgaaaagtcc
    14641 atatgttcat gcttcaaacg attcgagatt tcctctggcg accactgaaa atcgacaaat
    14701 aaatgacgaa caattgacca aactcacgga tcatttagaa tgtgataacg tccgcatttt
    14761 tttcttcgat aagcataatt tttttccgct ttggaaggtg aatagttttt ttcttgtttc
    14821 ttctcagttc atgtaaaata gtaaacgggc tatgctgaat gtcgttgcca attatactaa
    14881 tagaaaatcc ttcttcgtga agcagaaaga ttctttctcg ttcgagtata gtaagatgac
    14941 gatactggct cataattatt ttccttctag gagtgttctc gacaaactta tactagttgg
    15001 atatcgatta tgactcattt ttatgtgttg cacttaaagt gtaaattcaa ggtatagaaa
    15061 gaggttccct atgaaaaaaa tacctattac aatcttaact ggctatcttg gttcaggaaa
    15121 aacgatactt ctaaatcata ttctgtacgg tgatcacgga aaggaaattg ctgtcattgt
    15181 aaatgacata ggggaagtaa atgttgatgg ggtattagtc gacaagaaag gttttaaaag
    15241 aactggagaa aaacttgttt cgatgccgaa tggatgcatt tgttgtacat tacgcgaaga
    15301 tttgattgtt gaactagatc aattagtgca attttctgac ttgaatcaaa ttgttattga
    15361 agcttcagga atcagcaaac caattccaat tattcaagca attatattag ctaaaaaccc
    15421 tttagacatt gatttgaccc aacgattaga gattggtgcc attattatag tagtggatgt
    15481 aaaacgtttt tgggataatt tcgaaacggg tgaaaagatt caggaaagaa aagctgaagg
    15541 taatgtagtt gaagaagaac aggatattcg tggcttgtta attgatcaat tcgagtgttg
    15601 taacatatta cttttaaata atagtgactt agtgtcaacc aagacgattt acaaaattaa
    15661 cggtctaatt gctaagttac agtccaatgc caaaataatt aggacaatta aaggtgaagt
    15721 agacttatcg aaaattctaa atacaaatct ttttaatata gatgagactt cctattcagt
    15781 gggctggttc aaagagttag agcttggcta tcaaaatcat attcctgaaa cagaagaata
    15841 tggtatctca agttttatct atcgaaatcg gttactcctt gacgcacaaa aatttgccca
    15901 tcttacggat aaaaattttc ctggcaatat tacaagagca aaagggtttc tttggtttgc
    15961 ccaacatagt gatttttcga cgatctttga aacatcgggt agctataaaa gtattgaatc
    16021 attaaattat tgggtagcta gtttacctaa aattgagtgt caaatgatta tacaaaaaaa
    16081 tcccgaattg aaggaagcct ggcatccgtt gtttggtgaa cgcattaata aactagtttt
    16141 tattggtatg aagatggatc aacaatgtat taaggaacaa cttgagggtt gtttaactga
    16201 ttctcttaat gcagataatt tccgaaaaat ttcaccattt ccatggaaaa agtctaatat
    16261 gtttagccag atatcgttaa tgaaattttt aagaaaagct ggtgtcaaga taggcgatgt
    16321 cataattatt ggcattttga ttattacctc ttttattccc ttatttgtat tctctataga
    16381 aaaggagaat gaaacaactg aagatgctat caaatatgcc atcgttcgaa ttgatggcaa
    16441 ggaaatggat cgcttcgatt tggataagat caatcataaa ctcgtgaatt atcatcctgc
    16501 gaagggacaa tataatattg ttgagattaa agatggaaga attcgagtta aagaagataa
    16561 tagtcctgat caaatcgcgg ttagaactgg atggatctca aaacccggtc aaacgagtat
    16621 ttgtttaccc cataagttag ttattagtat tgagaaaaaa gaaagcacag attactacat
    16681 ttactaatct ggaaatagat gaggaggttt ttaataagaa aaagatatta ttattaataa
    16741 tagcaggggc tttattaagt gtgttaagcg cttgtggaca agaaaaagaa gcggtgacaa
    16801 caggcacaag tatctttaac gggacctgca atggtttagt taacagtcgg ttagattact
    16861 tattttggct cttcgtcaat gagtgttggt gaagaaattc aatagaataa tcgagatact
    16921 cacacggcta gacagctatc ttgtctagcc gtgttgttgt tctggcttta gtcagttggg
    16981 tagtattctt agttttctta aaatattttc ctcgatataa aaagattcgt tctcggtagt
    17041 tttgaaagtt tctatagcca taggcaattc gttttagtac tttgatatgg ttattcaaac
    17101 attctatagg gccgttggaa tagggaacgc gcaacgctcg tttgatttct gtttggtatt
    17161 tcttgaaggt cgtgaacact ggttggtagt gctctggcaa ctccttgtag tcttctttta
    17221 ggagcgcgac gaattcagga acatcttgtc ttctgataac ggaaagaaaa tcctggtaga
    17281 cttcgtagcc tcgctttaag gaatcatcat aagataatag ccgatcgact atctctgctt
    17341 ctgtcagatg atctcgaaat gacggacgcc agtagcgatg ctgacctttc aattcccacg
    17401 cattcttttg aagtagtttc cagtatttct ttaagtgctt cgaccgacga tccccttttt
    17461 taaaggtctt cattacttga attcggtggt tggtaaaagc ccgaccaatg tgttggctaa
    17521 tatgaaagcg atcggcgacg attcgggcat tcggaaaaca ctctttcacc aatgtgcgat
    17581 agggtgtata gaaatctgaa accactagac gcacttgttc acggactttt atagggtagc
    17641 gcaagaaata gtcgcgtaaa ggattgagtt gccgattttc cacaatatca atcagttgat
    17701 gggtcttgcc gtccatcatg acaaagctca tggctcccga gacatttttc actgatttga
    17761 attcatcgaa acacaccact tctggtaaca gctggctcaa agaaatggtt ttaggccgaa
    17821 ggctgcgaag tattcgaatg acagtggtcg gagaaatgtg cttcatacgt gcgatcaaag
    17881 acatagaaag aggttcgctt aatagctcaa gaatggcttg tttgactctg cgagcaatgg
    17941 agcagcgacg atcactaact gaatcttctg ctaaaaaagt ctttccacag tgtttacatt
    18001 tgaagcgctg tttgttaatc cgcagaatgg tcttgtagtt actgacatca ttcaataaga
    18061 tggatacttt tttccagccc tatttaatga tcgtttgttc attttgatag tggcgatgaa
    18121 agcattcctc cggttgatag gtcaatgtgc catcaaaaac aaaacatgtt tcgtcattga
    18181 ttcgctcttt gcgaaaggca tcttcatgaa agataagatt taggtctaga atatctagta
    18241 actctttggt aaactctgaa taggacatct tgaaacactc ctttgaatgg ttttcgtcga
    18301 ctttattcta aaggatttta aggtgtctgt ttgcgtttat tagaaaaaag aaaaagtatt
    18361 ggcaacagaa ggcgtcacca acactagata ttatagatcc cttattttct tcgcttcaaa
    18421 ttgtcaagga gtcatcataa attttgtatg gattcttttg tttgggtagt agtcttctaa
    18481 ataatcttgg attaacacct tcatctctat tttactttta tatttgttgg agtcgattaa
    18541 ttctcatttg attgatcgat gaaacgcttc aattactgca ttgtcaacag gagtcccagt
    18601 ttggctcact gaatgtgtga tgtggtggtc gttcaacagc tcttgataaa taaaagaccg
    18661 atgctggcta cctctatcgg aatgaattac cgtaggcaat tctccttaat aaatttgtaa
    18721 tggacggacg acaatctctt ttttcatgtg tgaatctacc atatagtaac gtagaatacg
    18781 agttgctaag tcaatatagg tggacaaata gaggcgaccg tcattacatg gaatgtaagt
    18841 aatatccatc acccaaacag catcaatttg gttctgttca aattatcgat tcaacatttt
    18901 tttaccattt ctttgattgt tttttctcta ccatgagaag atttgcgtcg atgaaatcct
    18961 cttgtgcata aattcatttc acgcataaga acagctacaa cacgtttatt gatttcgatt
    19021 ccttcatcgt aaagtttttg agtgatccct ggagatccgt atcgatcttt gtgttcgtag
    19081 aacactcgtc ttcctctttt agtcgtttat tttcctgttc aagaatgtag aaatctgctt
    19141 tatcaggtaa tctttttcct tgtccagcaa aagcattttt cccgtatcga cgatattctg
    19201 aaacccatcg ggaaattgtt ggacgagaga tattgtattc ttcagcaatt ttcttggtag
    19261 ttactttttt ctcaaggata tcctgtgaaa tctttagttt aaatttacga tcatatgttt
    19321 ttctcaatag agttcattcc ttccggtaac gtaactgata gtcaagaaaa gtgtatcata
    19381 gcccaactta gtatcagggt ttgtcaacta aactgtggaa gttgatagat aagagttttt
    19441 aacaaccaaa attctcttga ctatttttga aatcagacaa attttgattg gacacatagt
    19501 tgaataaatc taccgtcgtt acggaaggta aatcgcatgc ttttcaattc tagaggagaa
    19561 gtcctcgttg aagtaccata gcccagttct ggattcgacc tcctgcccac ttcatacgta
    19621 aataaagtaa ttttaggagt gagactgtga caaaagttat ttttaagcta aaaacgaatt
    19681 aaaaaacgtt gataattaaa atcattcgtt ttagattcta tgaatcatta tagaacgctg
    19741 taaaatcgat actttataat tataaagaag atagtttcaa atttattgtt cgaaatttga
    19801 aactatcttt ttacttatgt cacagactcc cttagttgag ttattcttta atttcgaatt
    19861 tcgagatagc tagatatatt gcaagcatac attcttgata acaatcttga tcatattttc
    19921 cagttctcca ggattgctta tgaatcaaag agctaaatct tttgagaatt gcctcaacag
    19981 cttcttcatt tccagccttt gctaaaatga aatcttcttt taaagaattc ataaatctac
    20041 cttcttccca tggacttata atctatttct ttgggaagga ggagaacgag caaagaagca
    20101 tacaaaatta gttattatgc agatattaaa ccagcatttt ttgaattttt ttgtaaagtc
    20161 gatgttttaa atttgtcgca ccctgtcttg taatccccag aacctttcca atttctttat
    20221 cagttttatc aaagataaat ttttcgataa gaaaaatttg ttcaggttct ttcaacaaag
    20281 gaaggatttt tgctaatttt atatcttcaa caaggagagt cacatttaaa acattaatcg
    20341 gttgtttaaa ttttatagta tctttagtaa acaaatatgg tgggagttga tcaagttgtt
    20401 catgtttttg aatttcttct tgttttttat aataagtatt agcggtgttc aaaatgacct
    20461 tttcaaagaa tcgaatcaac tgggcattta cgcgttcgtc gttatctgac atattgcaca
    20521 cctcctccat taagatttaa agggaaaaga ggcaaaaaac acgaaaagaa gctaatgcaa
    20581 ttatgctgcg ctgcgaataa cttctataag ctcacatgca ttgataggac tgctgatgtt
    20641 tattttcaaa aatgtttttt tagttgataa tggatagtta tctttatact tagaaaagaa
    20701 aattcatata tcttcttaaa tatcaaacat gtccttatgg ctaagaaaat gtcttgagct
    20761 ttttacgtat aataaatctt ttctaaaacc tccttttgga taatattctt attcgtgtat
    20821 agattgtatt ttttatttct tcccccttca taattaacaa ctagaaaaga gttcgaatag
    20881 acacgtattt gaacgaaaat ttttattgga ttcccaaatt ggaatattta gaaacgaaaa
    20941 attcctatta tctgtatagt gcttagataa taacggatca taataattat tgtctttgaa
    21001 ctagatatat gtaaatagta ttcatcatga tgaaaaatga tgagttctcg gtgataaaat
    21061 gtacttttta cggtggaata tacatgaaag cgataagtgg atattttata atgaagtaag
    21121 ttagcttatt ttgacagagg aatagtattt tagataacgt ataaaatact atttaatgat
    21181 gtagaaagga aagagagaaa caaaagtgtt ttcagataaa gaagtcttat taaagtctct
    21241 tcatgtttgt ttaaagttac ctatcgtggt ttttaattcc gaattcagta ttataaaaga
    21301 gtatcgctca gatagaacta cttatttttt ttatgattat aaaaagttac taactgataa
    21361 tattaaaaaa actaaagatt tccatttctt tactggtgat tttaatgaga tgtttcttct
    21421 atacatgttt aaaaccagat attacttatt tggtccattt agagcaaata atattgataa
    21481 agtttttttt aaactaaaaa tgaacaattt gaatgttgca atggctgata gagaacgact
    21541 ctataattca ttgcaaaatt tgactttgta ttctttagga gacattcgag atattctgat
    21601 tttagttcat tattttttta ctggaaaaat agaagatcta ttccatgaac cgttaattga
    21661 atatacagaa aatctaagta aaactatcga gcaaatcaaa atagataatc tgttatcaca
    21721 aaattacgat ccagaaatat atctgtttct ttatgaaaat aaaatactgg aatatgttaa
    21781 aaatggagat attcgaaatt tagaaaacat ggtttttaat ctgagtaatg gaataattcc
    21841 aagtgttagt ggagatacga ttcggtctga aaaaaattat tctatcattg tttttgaaaa
    21901 gttagcacaa acgtctataa cattaggaat ggatataata gaagcgtatc aaagtagaga
    21961 tgctttgata caagaaaacg aattagctgt atcgttacct gaggtcttaa aagtgaggga
    22021 ttctgggatt gtttattata ctaaggaaat cggaaaaact aagatagagc atctttctcc
    22081 cttaatttct tctgtcgttc aattcatcgg cttaaatatc tataaaagaa ttactgtaaa
    22141 ggaaatagct aattattttt ctgtaagtga aactaaatta agagaaagct ttaagaatga
    22201 aatgcatatt acgatttata attatatctc taagcgaaaa atttctacgg ctaaaataat
    22261 gttgaaatca aaccatacta tcagtgaagt ttctttaggt ttaggatttt cagattcttc
    22321 acacttttcg agagtattca aaaagtacgc aggtgtatct cctaagcaat atcaattagg
    22381 tttagtggac aatttcaaca acattagctg agaggtttta ttttattatg agactatatc
    22441 atttacgtag tttggaggag ttgaggaaat tggattttaa caaaaaattt ttttgggaaa
    22501 tttctgattt gagttgtttc gtagaatctg agcgtctaag taatggtaat aatagtgtgt
    22561 taaatgaata tgacaaagta gtaattaata aagtaaaaag tttatgtgaa gaagctagag
    22621 aatctatcag tcatattaat ggtagcaatt ttaaacaaac attatttaat ctgattaagc
    22681 tagatgctaa aatatcaagt tatttattct ttttgataca tgatgaattt atggattatc
    22741 aaaaattgga tcagttaatt gaaagagaaa gctgggaaga ttattacgtg ggtctaactt
    22801 tttcagaaaa gttaaacaac tacaagttaa tagattataa gtatttgagt gaaagcaatt
    22861 aattattcat gtagagtact agacacttta aatatggaca attcaattat aaagaaaaag
    22921 aatcaataat ttaatgaggc tgtgacaaat gtcatttttt gtttcgctaa aaacgagtga
    22981 aaaaatgttg ataactccaa tcattcgttt aagattctag gaatcattca gaaatgctgt
    23041 gaaaccgata ctttataatt ataaagaagg tgatttcaaa ttccgaagaa ggaatttgaa
    23101 gtcacctttt tacttatgtc acagtctcat tcagtaatgc gggataaggc tccttattcc
    23161 gttatttgaa tactctgaag tttaatctaa gaaagggtaa tattttgaaa acatgcttca
    23221 tcgcatagaa atgaacttaa tttcagaaag aatacataca tatcttttta tatgttttca
    23281 gctgctgaat ggttaaaaag cacaaatatc cgaaaaaaac atgaagaaat gtactttttt
    23341 tgttgtaatg ttcatgaaat tctctaagta aattagttaa cataaggtta gtaagattcc
    23401 tattctgtga ataatacgta tcagatttat cttttattct tactaggaaa gtaattatta
    23461 agggagttga tttgaataat ggttagcaag aataataaga gagtatttct agaaaaaaca
    23521 aagaaacgag tattgaagta cagtatcaaa aaattaagtg ttggggttgc atctgttttg
    23581 attggtgttg gtcttgtctt gggaacaact gaactagtta aagcacaaga tgaaatttct
    23641 ccaagcagcc cacttgaaac agcgataagc tccgtacaag taggtgacaa agtagcttcg
    23701 gggaatactt ttcaggagaa tcctggttat acgaaaaatt ataacttttc ggatttacaa
    23761 tttagtcctc aagaactaac tggagacaca ctaaaaggca atacaattgg atttgaagtt
    23821 tacggaaaac ataatattgc agcatcaact aaaaactggg aaatccgtct tcaattagat
    23881 gaacgattag ctaaatatgt agaaaaaatt caggtcgatc caaagaaagg aattggatct
    23941 tctagacgga cctttgtaag aattaatgat tcgctgggta gacctacaaa tatttggaag
    24001 gtcaattata ttcgtgcaag tgatggatta tttgctgggg cagaaacaac cgatacgcaa
    24061 accgctccta atggggtaat tacgtttgaa aagagcctag atgaaatttt taaagagata
    24121 ggcatagaca atctcaagac cgatcgttta atgtatcgaa tctacttggt aagtcatcaa
    24181 gacgatgata aaattgtacc gggtattgat agtacgggct actttttaac agattcagat
    24241 gatttctata atagcttaga tgtatctgaa aataatcctg accagtttaa acatggatca
    24301 gtaagtgcaa aatatgagaa acctaatact caaacaaaag atggttcagg atccacaggt
    24361 gctaatggag ctataatctt agatcataag ttaacgaaaa attataattt ctcatattca
    24421 tcttccgcaa agggcacgcc ttggtatgct aattacaaaa ttgatgaacg attagttcca
    24481 tatgtagcgg gtatacagat gcacatggtt caggctgaca aagtaacata tgatgtttcg
    24541 tttgaatccg gaacaaaagt agctgattta gcaatcgagc gacgggaagg tcatgaaaat
    24601 tatggcatag gttcaataac taataatgat ttaactaaac ttatcgattt tgctaatgca
    24661 agtccacgtc cagtcgttat tcgatatgtt ttacaattga ctaagccatt agatgaaatc
    24721 ttagaagata tgaaagcaac agcacaagtt gaagaaaata aaccatttgg tgaagatttc
    24781 atctttgatt cttggttgtc ggatacgaat aaaaaattaa tccagaacac ttatggaaca
    24841 ggttattatt atttgcaaga tattgatggt gatggaaacc ctgacgataa agaagagagc
    24901 ggagacacga atccatatat cgggaaacct gaattagaag aagtatatga tgttgacaca
    24961 acagttaagg ggaaagtatt catccacgag ttagcgggaa caggtcacaa agcccaactt
    25021 gttgataaag aaggtactgt attagcagaa aaaactatcg ctccaaatga aaaagatggg
    25081 gctccaattt cagatactgt agaatttgaa tttacgggtg tagattcaag taaattaatc
    25141 gcgaaagatg aattaaaaat ccaaatcgtt tctccaggtt ttgataaacc agaagaaggt
    25201 tcaaccgtta ttaaggaatc accaaaagcg gttgataaac aaaccgtggt agttggattt
    25261 aaaccagatg ctaaagaatc aattcggaat aataaaaact tacctgaaga tgcagagtat
    25321 tcatggaaaa cagagcctga tacttctaac gttactgata gtacgaaagg tattgtaact
    25381 gttaagatcg gaaatcgaac tttcgacgtg gatgtagagt ttgctgtaaa agcttctcaa
    25441 gctatggaaa atgatgcaac atacgtacct ataacaacaa ctccagaaac gacagttcaa
    25501 agtggaaaac ctacatttga taaaccagat gttcctttag ctaaagatgc cttttcaatt
    25561 tcagatgtct ataataagga cttcggtaat gcaagtgttg atgcaaacac tggtattgtt
    25621 acattcactc cagctaaagg tgtaggagaa tcggagccga ttactggaac aattcctatt
    25681 aaaattgttt accaagatgg ttctgtaggc acgaccgatt tagcagtaac tgtaagtaaa
    25741 gatatttatg aaaatccagg agaaaacatt cctgcaggct accacaaagt aaccttcacc
    25801 gcagg
//
LOCUS       DAFEPQ010000034        25496 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.034, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000034
VERSION     DAFEPQ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.034"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            127..3654
                     /locus_tag="LP543_001558"
     CDS             127..3654
                     /locus_tag="LP543_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBL2321727.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="LP543_001559"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="LP543_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBL2321728.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="LP543_001560"
     CDS             7442..7783
                     /locus_tag="LP543_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2321729.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="LP543_001561"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="LP543_001561"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBL2321730.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="LP543_001562"
     CDS             9140..11560
                     /locus_tag="LP543_001562"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBL2321731.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKEINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="LP543_001563"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="LP543_001563"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBL2321732.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="LP543_001564"
     CDS             12994..13731
                     /locus_tag="LP543_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL2321733.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="LP543_001565"
     CDS             13745..14560
                     /locus_tag="LP543_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBL2321734.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="LP543_001566"
     CDS             14573..15220
                     /locus_tag="LP543_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL2321735.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="LP543_001567"
     CDS             15236..15892
                     /locus_tag="LP543_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL2321736.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="LP543_001568"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="LP543_001568"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBL2321737.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="LP543_001569"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="LP543_001569"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBL2321738.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="LP543_001570"
     CDS             18234..18752
                     /locus_tag="LP543_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBL2321739.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /locus_tag="LP543_001571"
     CDS             18859..19359
                     /locus_tag="LP543_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL2321740.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19639..20559)
                     /locus_tag="LP543_001572"
     CDS             complement(19639..20559)
                     /locus_tag="LP543_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBL2321741.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20724..21737
                     /locus_tag="LP543_001573"
     CDS             20724..21737
                     /locus_tag="LP543_001573"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBL2321742.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="LP543_001574"
     CDS             complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="LP543_001574"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBL2321743.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22822..23262
                     /gene="fabZ"
                     /locus_tag="LP543_001575"
     CDS             22822..23262
                     /gene="fabZ"
                     /locus_tag="LP543_001575"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBL2321744.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23377..24138)
                     /locus_tag="LP543_001576"
     CDS             complement(23377..24138)
                     /locus_tag="LP543_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL2321745.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24327..25421
                     /locus_tag="LP543_001577"
     CDS             24327..25421
                     /locus_tag="LP543_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBL2321746.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8449 a         4424 c         5382 g         7241 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aagaaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
    19501 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt
    19561 ttagagcgtg ggacaaaagt gttctttact tttgtcccac gctcttttta gtgagattcg
    19621 ttgcatcgtt tgtccaaatt attttccgac agcaattgga ctgttcggaa tcgtgaaacc
    19681 agaagctgtc aattctttga cataggagct caagaactct tcttttaagg catattgttt
    19741 tccgttcaat gcgtagcaga ccgtacgaat cgcaaaattg ctgtttccaa gatccactaa
    19801 tccaaagata gaaggttcag tttggagctc ttcttggtat ttttcagcta atgtacgatt
    19861 tactcgatca atgatctcat agattttgtc atatccttct tcaggaacaa tccgaatatc
    19921 taaaagtact tgcatatttg cacgagagag attgctgatc gtggtgatgt ttcgattcgg
    19981 gatgaaatgg acagtaccat ccgttgcttt cagctgaagg gtacggatac caacagatac
    20041 gacggttcct tcaatcgtta agtttgctag cttgatgtag tccccaacat ccatctgttg
    20101 ttccatgata ataaaaaatc cggtaataac atcactcatg aacccttggg caccaagacc
    20161 gattgccaca cctgcaattc cggcaccagc caaaagtgat ccgatcggca caccgatagc
    20221 agataaaatt gcatagataa aaaagaaacc aagggtataa tgaaaaatag tatttaaaag
    20281 actatgtaaa gtctttaagc ggctctcgtt tagaatggct tttttagaat actgattaaa
    20341 agacttatca atcagatatt ttcccacacg gtgaagaatc caaaaaagga cgatcaaaaa
    20401 aatcagatat aaactttttt ggataaaggc agccaaaatg gcgtcccaat caatcccgtc
    20461 ccaaaaccgc tgaaacgcat ttactttgct gacagcttga tctgtcaatg tctcgccaga
    20521 agagtctgtc gtaccggcag ttgctaataa tgaaaacata aaatcctctt ttctaaaatt
    20581 tgattctttc tgtattgtac caagaagttt tcaaaaaagg aaatagcaaa aataactata
    20641 atgaaggaaa aaagctgaaa ataaaatgac aaataatatg gataatgata aaatgtggag
    20701 aaaataacga aggtggtaag tatatgcaaa tcgattggaa caatctaggt ttttcttata
    20761 tcaaaactcc ttggcgtttt attgccaaat ggaaagacgg acaatgggaa gaaggagaat
    20821 tgacagaaga taattacctg acgattcatg aaggctctcc agcgcttcat tatggtcagc
    20881 aatgttttga aggtctaaaa gcttatcgtc gaaaagacgg caaggtcaat ctctttcgac
    20941 ctgaacaaaa tagtcgacgg atgaatcaaa gtgctagacg attgctgatg ccaatggtcc
    21001 ctgaaaagat gttcgttgat gctgtcaagc aagtggtaaa agcaaatgaa gattttgtgc
    21061 cgccctatgg aacaggcgct acattatact tgcgcccttt actgattggg gtcggtgaaa
    21121 acattggtgt ccatccagct ccagaatatt tatttgtcgt cttctgtacg ccagtaggag
    21181 cgtatttcaa aggtggacta aaaccaacga attttattgt atctgattac gatcgtgctg
    21241 ctcctaaggg gactggtgca gcaaaagtcg gcggaaatta cgcagcgagt ttacttccag
    21301 gaacagaagc aaaagaactg gattactcag attgtgttta tcttgatcca gccacacata
    21361 cgaaaatcga agaagtaggt gcagcaaatt tctttggaat tacaaaagac ggaaaattca
    21421 tcactccgca atcgccctct atcttaccaa gtatcacgaa atattccttg ttgcagttag
    21481 ctgaagaacg actaggactg caagcaattg aaggagatat tttcattgat gatctagatc
    21541 aatttgcaga agctggtgca tgtggtacag cggctgttat ttctccaatc ggtgggattt
    21601 atcacaataa caatcttcat gtattttata gtgaaacgga agtaggacca gtaacaaaac
    21661 ggttgtatga tgaattgaca ggtattcaat ttggagacat agaagcacca gaaggctgga
    21721 ttgaagtcgt ggaataattt caaagtaatg agttgaaaaa aacgtaaaaa gaaaaaatca
    21781 ggaagagact ttaaaaagtt ttttcctgat ttttatatgt tttactttaa agaatgcaac
    21841 aaaagtttat gaaagatgga cacctttgtc aacgtagata atatcaccga tgattccgct
    21901 ggaaagtggg ctgattaaga aagcacaagc attaccgact tcttcgatcg tcacaccttg
    21961 ctgatcaggc gtacgagatt cagataactg gatcagtttt tgatagtctt taacacctgt
    22021 gacagctaat gttttgattg ccccagctga gattccattg acccgaattc ctttaggcga
    22081 gaactcagct gccaaataac ggatagcagc ttcaagagaa gctttcgcaa tacccatcat
    22141 gttataattc gggattgcgc gttctgaacc tagataagtc atgctaacga tcccgctgtt
    22201 tggtgcaaga aacggttgtg cgtattttga tacagcaatt aaagagtagc tgctgatatc
    22261 ttgagctaat tgatagccgt ctctggaaat atcagacaca tttccggaca attcttcttt
    22321 gttggcgtaa gcaactgcat ggacaaggcc gtggatctcc ccagcatatt caccgatggc
    22381 attcattgct ttttcgatgc tttcgtcgct agaaacatca cattctacag tgaatgcttc
    22441 ttcgccgacc aatttgacta atgatttttt cattcgttca ttttggtaag tatagataat
    22501 ttctgcccct tgtttcaaca ttgcttcagc acaaccccaa gcaatgcttt ttttgttagc
    22561 gacgcccata atgacgattt tttttccttc tagaaatgac ataatgactc tcctttgttt
    22621 ctgataaaaa tttgatagtc gtaaaaaaat agagaataat agctaaaata acttcttgat
    22681 ttcttaatta ttagtcaaaa tgttttgatt gtcaaactat tttttcttaa attaaaaaaa
    22741 gattgctaaa tatttaggaa tatgatactt tgatagtcaa agtaatgaaa atggcaattg
    22801 cctgaaagaa ggagtttaat tatgtcttac gtattatctg ctactgaagt aatggaattg
    22861 attcctaatc gttatcctat ttgttatatt gattatgtag atactttaga accaggaaaa
    22921 aagatcgttg cgacaaaaaa tgtaacaatc aatgaagatt ttttccaagg tcactttcct
    22981 gacaatccag tgatgccggg tgcgttgatt ctagaaacac tggcacaagc aggttcgata
    23041 cttatcctaa aatcagaaga gtttctagga aaaagagctt atatcggtgg gatcaataag
    23101 gccaaattcc gtcaaaaagt tgttccagga gatgtgctga aatgtcattt tgaaatcacc
    23161 aaaatgaagg gatcagtcgg tacagcgtta tctgcagcat atgtagatga taaaaaagtc
    23221 tgtgagtgcg agttcacgtt catcgtcaac caaaatgctt gactataaaa cctgatatag
    23281 caaaacaaaa ggaagcttac ttgcttttta tgtaaaaaga agacgcctga atcctagcat
    23341 acgttgtcat ctatgcagga ttcagacgtt atttttttat ttttgttttg cttcttgcag
    23401 tatattgatt ttatcgattt ttacttcttc ttttggtttg tctgaactat ctgtttcgat
    23461 atttgcaata gcatccacga catccattcc ttcaatcact tggccgaaga cagtataatg
    23521 gccatctagt gaaggataac cgccgttttt ataagcgtcg atgattgctt gaggatagtc
    23581 gtcttttaaa agaccatcat gcatatcatc tgtattttga acgataaaga actggctgcc
    23641 gttactgtta ggatcttgtg agcgagccat agataacgca cctcttatat tataaaggtg
    23701 gttgttgatc tctgtttcaa atccttctcc ccaaatgctt tcccctccag tgccatcgcc
    23761 tttaggatcg ccaccttgga tcatgaaatc tttgatgacg cgatggaaag tcagtccgtt
    23821 gtagtaaccg tcttttgcat gtgtcataaa gttttcaacc gcttttggtg cgtgttcggg
    23881 aaataatttg attttgattg tgcctttatt cgtcaccatt tcgaccagat cttcattttc
    23941 ggcgacatct ttcgtcaatt gtggtaattc caatgcgttc aaatcgacgc tactggcagt
    24001 cgtgctttcc gcagtagatg tactttttga actttctgta gacgaactag aagaagtcgt
    24061 gttgtttgca gaacaaccag cagcaaacag caatgctgct gctaaactaa tacttaaaat
    24121 aactttattt ttcttcataa ctgttccttt ccaatttact attttgttat ctttaatgat
    24181 aacaaaatag agggaaaata cctacttaaa ttattggaat ttatcattta ttttaaaaga
    24241 aaaagaaaag gtgtagacca tttactttgt gtttgctata atgtaagtgg atacaaaaac
    24301 gaaaagatgc aggaggaaat ggtaatatgg gaaaattagg aatctcgatt tatccagaac
    24361 ggtcaacatt tgaaaaggac aaagcgtact tggatttagc tcataagtat gggtataaac
    24421 gggtttttac cagtttgctg cagatcaatg atgataaaga aaaagttctt tcagaattta
    24481 aagaagtagt tgattacgcg aatagcctag gcatggaagt aatggttgat atcaatccag
    24541 cgttatttga acagttggag atttcttacg atgacttatc ttttttccat aagatgggtg
    24601 cctacggggt ccgcttagat atcggattca caggagcaga agaagcaaaa atgacgcgca
    24661 atccttttgg tatcaaaatc gagatcaata tgagttcggg cacgaattac gttgacaaca
    24721 tcatgagtta ttcgccaaac acggataatt tattaggttc tcataatttt tatcctcatc
    24781 gatactctgg tttaggatac gaacactttg ttttttgttc agaaaaattc agaaaatata
    24841 atttgaatac gatggcattc gtcaattcgc aatctgctga gtttggccct tggcctacgc
    24901 aagatggctt gtgcacgctg gaagaccacc gagatcttga aattgcgaca caagtgaagc
    24961 atttaatcct tacaggattg attgatgata tttctattgg aaatgcgtac gcaagtgaag
    25021 aagaattgaa agaaatggca gaagcattta acgcggatta tccaacattg aaagtagata
    25081 cggaagaagg tattacagaa aacgaacgaa tctgcttgtt tgataacctt catagttatc
    25141 gaggggatcg ctcagaatat atccttcgtt caacgatgac tcgtgtatac tacaaagata
    25201 aagatttccc gcctcataat acgcgagata tgcatcatgg ggatgtactg attgacaatg
    25261 aagggtacgg tcaatacaaa ggagaaacac aaatcgcctt gaaggatatg aaaaacgacg
    25321 gacgtgtcaa tgttgtagga agaatctccg atgacgaatt attcctcctt gactttttga
    25381 aaccatggtc aagctttaaa ttaattgaaa ataaaaaata gataattagt tattgcagaa
    25441 ataatccgct agaaatagcc agttatttct gcttttttat tttgtttttt tatagg
//
LOCUS       DAFEPQ010000035        25378 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.035, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000035
VERSION     DAFEPQ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.035"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(3..1991)
                     /gene="treP"
                     /locus_tag="LP543_001578"
     CDS             complement(3..1991)
                     /gene="treP"
                     /locus_tag="LP543_001578"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBL2321747.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLVYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            2214..2927
                     /gene="treR"
                     /locus_tag="LP543_001579"
     CDS             2214..2927
                     /gene="treR"
                     /locus_tag="LP543_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBL2321748.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            3004..3450
                     /gene="msrB"
                     /locus_tag="LP543_001580"
     CDS             3004..3450
                     /gene="msrB"
                     /locus_tag="LP543_001580"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBL2321749.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            3620..4222
                     /gene="ruvA"
                     /locus_tag="LP543_001581"
     CDS             3620..4222
                     /gene="ruvA"
                     /locus_tag="LP543_001581"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBL2321750.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            4235..5236
                     /gene="ruvB"
                     /locus_tag="LP543_001582"
     CDS             4235..5236
                     /gene="ruvB"
                     /locus_tag="LP543_001582"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBL2321751.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            5265..5843
                     /locus_tag="LP543_001583"
     CDS             5265..5843
                     /locus_tag="LP543_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL2321752.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKVLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            5895..6377
                     /locus_tag="LP543_001584"
     CDS             5895..6377
                     /locus_tag="LP543_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBL2321753.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            6494..6868
                     /locus_tag="LP543_001585"
     CDS             6494..6868
                     /locus_tag="LP543_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321754.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(7668..9020)
                     /gene="brnQ"
                     /locus_tag="LP543_001586"
     CDS             complement(7668..9020)
                     /gene="brnQ"
                     /locus_tag="LP543_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBL2321755.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            9167..9757
                     /locus_tag="LP543_001587"
     CDS             9167..9757
                     /locus_tag="LP543_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBL2321756.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(9885..11234)
                     /locus_tag="LP543_001588"
     CDS             complement(9885..11234)
                     /locus_tag="LP543_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2321757.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(11402..12142)
                     /locus_tag="LP543_001589"
     CDS             complement(11402..12142)
                     /locus_tag="LP543_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL2321758.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(12155..13747)
                     /locus_tag="LP543_001590"
     CDS             complement(12155..13747)
                     /locus_tag="LP543_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL2321759.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(14315..14611)
                     /locus_tag="LP543_001591"
     CDS             complement(14315..14611)
                     /locus_tag="LP543_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321760.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            14787..15512
                     /locus_tag="LP543_001592"
     CDS             14787..15512
                     /locus_tag="LP543_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBL2321761.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            15505..16347
                     /locus_tag="LP543_001593"
     CDS             15505..16347
                     /locus_tag="LP543_001593"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBL2321762.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKAVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            16350..16871
                     /locus_tag="LP543_001594"
     CDS             16350..16871
                     /locus_tag="LP543_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBL2321763.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(17124..17687)
                     /locus_tag="LP543_001595"
     CDS             complement(17124..17687)
                     /locus_tag="LP543_001595"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBL2321764.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            17938..18207
                     /gene="rpsO"
                     /locus_tag="LP543_001596"
     CDS             17938..18207
                     /gene="rpsO"
                     /locus_tag="LP543_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBL2321765.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            18395..20521
                     /gene="pnp"
                     /locus_tag="LP543_001597"
     CDS             18395..20521
                     /gene="pnp"
                     /locus_tag="LP543_001597"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBL2321766.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            21031..21297
                     /locus_tag="LP543_001598"
     CDS             21031..21297
                     /locus_tag="LP543_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL2321767.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            21474..23432
                     /locus_tag="LP543_001599"
     CDS             21474..23432
                     /locus_tag="LP543_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBL2321768.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQGWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            23786..25087
                     /locus_tag="LP543_001600"
     CDS             23786..25087
                     /locus_tag="LP543_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBL2321769.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
BASE COUNT         8172 a         4806 c         5204 g         7196 t
ORIGIN      
        1 gactaagatt ttacttcagc tttaccgact gcttcgcccg ggcgaacaag tcctgattgg
       61 tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag caacaataat gtcagttttc
      121 ttgccagctt gttctatttc tggcaaatcc atttcagcaa tcagggtctc tggtgtgaca
      181 aggtatcctt ctttcacgaa tacattgaaa ggaacaccct ttagttcaac agtatctaat
      241 cccatgtgaa caagtacttc cagcccctca ttagaaagaa taccaatggc atgttttgtt
      301 tcaaagacac tcgtcacttt accgtttact ggtgcataga ctttgccgtt tgacggttcg
      361 acagcataac cgtcacccat cattttttgt gagaaaactg ggtcatttac ttcagttatt
      421 tccttgatgg tccctgcagc gactgcgaat aatgtttctt ttgttgcaac tgccgtacca
      481 gttgctgaaa caggttcgaa agactgtggc gttgctgttg cgccatttgc tgtttgaagt
      541 tgtacatcag cagcattttc aggaacagca ggatcaatct tattcaaaat tccctgacgt
      601 ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa tgatcattgc gatgatgaaa
      661 ggtacccatg tttctgcttg gatagctagt attcctggca aaccgcctac tccgatcgcg
      721 ttagctcgaa cacccatcaa gttagcaaac atgccagcta atccagagcc gaccattgca
      781 gcgacgaaag gatagacgta tttcaaattg attccgaaca tagcaggttc agttacgcct
      841 aagtaacaag aaatcattgc tggaatcgag atttgttctt cttttttatt cccacgatgt
      901 aagaagatga tggctaagac tgcagagcct tgggcgatat ttgataaagc aatcatcggc
      961 cataaattcg ttgatccgaa atctgcaatc agctgcatat cgatggcatt ggtcatatgg
     1021 tggagaccag taatcactaa aggagcatac aggaatccga atacggcact aaacaaccag
     1081 ctgatagaag acgtcaatcc agtatttacg atatttgaga tccagcttcc gactgtccag
     1141 ccgataggac ctaaaataac gtgtgcagct aaaacagttg gtaacaatga aaacaatgga
     1201 acaaaaatca tcgagatcga ctgagggata tatttgcgga agaagatttc tagatacact
     1261 aacataaacc ctgccagcat ggctggaata acttgcgctt gataaccaat catctggact
     1321 tgagcaaaac caaaatccca aaatggaata tctgctgcgg ctgtggaagc tacactataa
     1381 gcatttaata attgcggaga aactaacgta attcctaaaa cgattcctaa tatttgtgta
     1441 gtacccatct tttttgcaat actccaagtg atacctactg gaagaaaatg gaaaattgct
     1501 tcacccggta accacaaaaa ggcattgaca ccattccaaa acttcgagtg ttcaacgatt
     1561 gttccaccaa gactttcaaa ctggatacct tcaagtacat ttctgaatcc aaggatcaat
     1621 ccaccgacaa tgattgcagg aatcagtggt gtaaagattt ctgctaaaac ggcaatagca
     1681 cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc cttgttcttt ggaaacccct
     1741 tcaactccag atactgcaac gaagtcgtta taaaaagtag aaacatcgtt tcctatgatg
     1801 acttggaact gtcctgcatt cgtgaacatc ccttttacgc taggtatatc ttcgatcgct
     1861 ttttcgtccg cttttcctgg atcattcagg acaaaacgca tacgagtcgc acaatgacta
     1921 actgcagcaa tattttcttt tccgccgata tcatgtaaca gcttctcggc atctgcttga
     1981 tattttgcca tatcaataaa cccctttctc ttaacttgtc tatgcaagtt gtttcttcat
     2041 ttactatact ttttttcgga gagagttgca agcgttttat taaaaagtac gagagaaaat
     2101 gatatcgttt taaaaagctt aaaataagct tttcttaaaa ataggaacat tgaaatacaa
     2161 aaagagctgt agtatagtga agaaatgatg gaagaacgag tgagggaaaa aacatgaata
     2221 agttcaatga aattttttta gatttggaac agaaaatttt agcgaaagaa tatcctccgc
     2281 attcgctttt acccagtgag aaccaattga tcaaaattta tggtgtttca cgagaaacaa
     2341 ttagaaaagc gctgaatctt ttgactacgg aaggttatat tcaaaaaaaa caaggaaaag
     2401 gttcgatcgt actggatcgg aatcgcttcg actttcctat ttctggattg actagctata
     2461 aagagctaca ggagacccaa cgtattccaa gtgaaacgat catccatacg ttagaagaaa
     2521 cagaagtgac aaaagcattg aacgagatca ctggctggga gatcggtgcg cctgtttggc
     2581 atttgatacg cgaaagaaaa atcaatggcg aagtagttat attagacacc gattatctat
     2641 taaaggaaat cgttcctatt ttaactccag cacaagcaca aggatcgatc tatgaatatt
     2701 ttgaaaatga gctttctttg acgattgctt acgcgcaaaa ggagatcaca gtagaagaag
     2761 taacggaaat catgaaaaca gcgatggatc ttcacgatga cacttatgca gtagtagtca
     2821 gaagcctcgt tcatctggaa gatacccgct gtttcgagta ttctgagtcg gttcaccgat
     2881 tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa agtgtaaata gttcaaaaga
     2941 aagcaaattg ttggcatctg tccactttgg cgctatgata ggatcacaaa gggggcattt
     3001 ttaatggata aaccaataag accagaaaaa gaagaactga aagaaaagtt atcacccatg
     3061 gcttatgcag tcacacaaga aaatgcaaca gaacgaccat ttagcgggaa atatgatgat
     3121 ttttacgaaa aggggatcta tgtagatatc gtcagcggtg aaccgttatt cagctctgca
     3181 gaaaaatatg atgcaggttg tggctggcca tcattcagca aaccaataac gaaaagacaa
     3241 gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa tagaagttcg aagcaaagaa
     3301 gcagattctc atctcggaca tgtgtttacc gatggaccag tagatcaagg tggtctacgt
     3361 tactgcatca attctgcctc actgaaattc attccatatg aaaagatgga tgaattaggt
     3421 tatggggaat tcaaatcact tgtagaataa tggaaataca caaagagcgg tacaatacgg
     3481 ggatttactc gatcgtccgc tcttttttta ttcaagcgat tgctatttga aagaaacgct
     3541 ccccagactc cttttttcac aaggtgaaat gtgctacaat aaagggaacg tatgtgtgca
     3601 aaagaaagag gactagaata tgtatgaata tctgacaggt gtggttacct ttatcaaccc
     3661 ctattacata gtagtagaaa ccaatggtat cggctaccag atcgcactag gaaatcctta
     3721 ccgctacagt agcaaactaa atcaaaaaat aaaaatttat gtccatcagg taatacgtga
     3781 agatgcgcat cttctgtatg gatttgattc gctagaagaa aagcaactgt ttttgcgatt
     3841 agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca atcatggcaa gcgaagacca
     3901 ctccggtttg attcaagcag tagaatccag tgatgtcact tatttgacga aatttccagg
     3961 agtagggaaa aagacagcac aacaaatgat cttggatcta aaaggaaaat tcggtgagct
     4021 gagtatcgat acacctttta gtctatttga tgaagcaaac acaaaagacg ctacagctct
     4081 atctgaagca atggaagctc tctcggctct tggttacagt gatcgagaaa tcaaacgtgt
     4141 agaaaaacaa ttaaaagaag tagataatca gacaacggat gagtatctac gccaagcact
     4201 caagctaatg atgaaaaaat aaggaggaga ccaaatgaca gatgaatact tgttatcacc
     4261 agaatctggc gaaaatgaac tgagtttgga aaaatctctt cgaccacagc tgctttctca
     4321 atatatcgga cagagaaaag tcaaacaaga acttcagatt tacatcgaag ctgccagaaa
     4381 tcgggaagaa cctttagatc atgttctgtt gtacgggcct cctggattag gaaaaacgac
     4441 gatggccatg gtcattgcta atgagatgac cgtaaatatt cgtacgacga gtggccctgc
     4501 tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa ctggaagcag gggatgtcct
     4561 gtttatcgat gagatccatc gattacctag agtggtagaa gagatgcttt attcagctat
     4621 ggaagatttc tatgtagata tcatggtagg acaagggcca accgcacatc cggttcattt
     4681 ccctcttcct ccctttactt tgattggcgc gacgacacgg gcaggtatgc tttctgcacc
     4741 tttacgagat cgatttggca tcatttctca tatggaatat tatgacgaac aggatctgca
     4801 ggagatcgtt ttacgttctt cggatatttt tcaaacagag atcattgaag aaggggcatt
     4861 ggaaattgct cgccgatcac gaggaacgcc tagaatcgct aatcgcttgc tcaaacgggt
     4921 tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat cgtcctatag cggatcaggc
     4981 acttactttg ctacaggtcg atcaggctgg attggattat gttgaccaga aattgttgcg
     5041 aacaatgatc gagttatatg gcggcggacc agtagggtta agtacgcttt cagtaaacat
     5101 aggagaagag cgtgagacgg tagaagatat gtatgaaccg tatctgatcc aaaaaggatt
     5161 tttaaagcgt acacctagag ggcgaattgc tactgcttat gcctatgaac attttggtta
     5221 cgactatcat atatgatttt tgcttttaga ttggaggaaa atgaatgagt gatacaacac
     5281 gtacgaaaca attgatcaaa aacgtattga ttgatttgtg ccaaacgcaa tcttttcgaa
     5341 aaatcagcgt ccaaaaaatc gcaaatgaag caaaaatcaa tcgacaaaca ttttattatc
     5401 attttacaga taaatacgat ttgcttcgtt gggtctattg tgaggatgca ttgcattatt
     5461 tagatccaca atgtctttca cttgataatt gggaagagca agcattgaaa gtgctgaaag
     5521 caattgctga gaagaaagaa ttttatgcaa atactgtgct ttctgaccca gaaattttac
     5581 aaaaagagtt ttctcaactg atccaaaaat catttatcaa gatttttgaa caaatggatg
     5641 aagaaagaca actttctgca agagacaaac tattttatgc tcgttttttt tcttacggtt
     5701 gcagtggtgt attagtcaat tggatcacga atgaatatga agaaacaccg atggaaatcg
     5761 ctatgcagct tttccgttta gccaaggata cggaattata ttcttatcgt ctttatgcaa
     5821 aagaagagtt ggaagaacag taatagaaaa attttttgtt ggaaaagcaa taatcgctaa
     5881 aaggagatgg ggaaatgaca aaaattttat ttgtttgttt gggaaatatt tgccgttcac
     5941 cgatggcaga aggcttgctg agaaaacgaa tagcaataga aggaagggaa gcagaatttt
     6001 ttgtggattc tgctgctaca agcacgtatg aagttggaaa gacaccgcac cctggaacga
     6061 aaaaaatatt aaaccaagaa aaagtagata tgacgaatat ggttgctcgg caaatcacac
     6121 cccacgattt tgaaactttt gattggatca ttggcatgga tcaagaaaat gtagaagaac
     6181 tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca tttgttttta agtagcattc
     6241 ctggaaaaga aaaagaaaat gttcccgacc catactatac caataacttt gatgagacat
     6301 atcacttgat cacagaaggc ctgaatcatt ggtggaatat ttggattgac gaaaataaaa
     6361 taacaaacaa tttgtaaagt tgacgctttc tttttaaggc gatgttagag catttccttg
     6421 tttttttgta aaatgttagg taaaataaaa ttaattaagt aacactttct tagctaaagg
     6481 aggagtgcat tcgatgaacg aagagacagt atcgccaatg aagaagctta cgaagttagt
     6541 tactcatact tcgtatcaac tttttgattc ttttgtaaat gacactgaag aacatgctaa
     6601 gaatgtacga ttgcaaggaa agtttattct tgagctgcaa aaagctgcta ctgaacatag
     6661 tctagttatc ttacaagtaa gagcgaatcg tttgagtagc aagtatgaaa cgatctcagg
     6721 ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg atccgcctac aaaacgaaga
     6781 gcagcagatc cggatggttc ctgtagaaca aattgaaaaa ataacaatcc tttcaccagc
     6841 aggggaacag gaacgattat caaaataatt tataccagat agaaaagcac gtatgagctt
     6901 gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt taagctgata tcagtaaaac
     6961 ggaacgtttt aggatgagta tcacagcatg acagcattgg gttgattagt tgaatcgatt
     7021 ggatggtaca agtttttttg ataaaaaaag gtcctgctag ccggggagct agcaggataa
     7081 ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg gtatgcttat ataatagtca
     7141 ctagttgtga atattttgtg acgaaagttt tcgaaagttg ttaagttttt ttgcgaaaac
     7201 tatttttata tcatatatat gtctaaaatc tatattataa aagaaatgtt gtttgatcag
     7261 cttctcttcg gtattagttc aaaacaaaac ggcaaagtat atggagctac tttgccgttt
     7321 tgtttctcta atactcaggt cagtttatag acgctcaata aacggtgttc aaaagccagt
     7381 ttctcggtat taagtcaaat ttatgcaaaa atgtgagaag ccattttcac aaaatatttc
     7441 ttaatacttg aaactaagcg gctttttcac gacctctcaa taaacggtgt tcaaaagtca
     7501 gtttctcggt attaagtcaa atttatgcaa aaatgtgaga agccattttc aaaaaatatt
     7561 tcttaatact cgaaactgag cggctttttc acgacctctc aataaacagt gttcaagaga
     7621 cagcctatcg gcaataaggc tgaacgtctc tttcacaacc tcttttatca atctgctact
     7681 tcttctttct tagcgaaact ccaaatcagt ccgataacaa aaccgataac tgctggtacg
     7741 atccacccca tccccaattt gaaaaacggc aagtagcttt ccgcaacata taaaagattt
     7801 tgaacaatag gtgtttgttt gatagtttct gggcaagcgt tcaacccatc aaaaatcgaa
     7861 gcaatcaatg taaaataagt agtcattcga taaacagccg tacgctgctt aaacaaagga
     7921 ccaactaaca ctagtaaaat cagtgtcatt gctaatggat agatgaacat caaaacaggc
     7981 aacgaaaatt gaatgatatt cgtcaagcct acgtttgccg ctaaacaagg aaggatacta
     8041 gcaagtgcaa caaagaatgc gtaatttctt tgcggaaaca aatctgtgaa tgtttctgca
     8101 aaagctgtaa tcaaaccaat accggtcttc aagcaagcta atataacgat aaatgctaat
     8161 aagatactgc cataagtacc taaataatga tcagcaatct gagccagcgt gatcccgcca
     8221 ttttcactta gagcaaagct acctaagctc atagcaccta aataagcgag caaagtataa
     8281 atgattccca tcaatataat gctgatcgca ccagatttga tcgtgtcttt agcgatttca
     8341 gcaggttttg ttacacccat gttttggatt gttgtgacaa tgatgatccc aaatgctaaa
     8401 gcagcaagcg catctaatgt gttatatcct tgagtaaacc ctgtgaaaaa agcatgctct
     8461 tgataatttg gctgaatagg cgcttgattt acagaaccta gtggattcaa aaaagcaagg
     8521 agaagcaaag cacctaataa aaccaaaaat gcaggattca ggaactttcc aacataatct
     8581 aatatctttg tcggttttct tgaaaaccac caagccagca caaaaaatac aatactgaaa
     8641 attgctagat atagcgtctg tctgctagga tcgataaatg gtgctaaacc aatctcgaaa
     8701 gatgtggttg caaggcgagg taaagcaaaa aacggtccga taactaagta aagcaagatg
     8761 gtaaaaacca tcgcatatgt ttgatttatc cgttttgcta attcatatac acctgagctt
     8821 ttcgatacac caatggcaat cacccctaag aagggaagac cgataccggt aaccaagaaa
     8881 cctagattcg cccaaaaggt agcagcgcct gcttcttgac ccaaatgaac gggaaagata
     8941 agatttccag caccaaaaaa tagaccgaaa agcatggagc ctataaaaag atagtcttta
     9001 aatgataatt tttttgtcat acataactcc tttacggttt ttttaaaagt aacaaaattt
     9061 cgaaaaatat gcaacaattt tcagaaaatt tcctatataa aacgacggtt atttaataac
     9121 gtaagtgata aaataaaagc gctgaatcat ctgaggagga atcatcgtga caaagtcgag
     9181 attagaagca tttactgatg gagtggtagc cattgttttg agagttcttg tattagagat
     9241 caagatccct aatacatcta gctttcattc tttatgggtc atccgaaata cattattagc
     9301 ttataccatc agctttatat ttgttgcggt gatctgggtg aaccatcatc gtatatttca
     9361 gatggcagaa cgaattaact accgagtggt ctggtcgaat atctttttgc tcttttggct
     9421 gactttatgt ccttctgtta ctagttgggt cgggcgtaac cctgaagctt tttggcctga
     9481 gatggcttat gtcttagtat acacgatgtg gagtttttct tttggtatct tgatgaggca
     9541 aatcatgaaa gcaaataaac cagattccca tgtcgtgaga gttttgtcaa aggaccgtcg
     9601 aagtcttatc tccatgatga ttaacttatg cttgattgcc ggtgtctttt ttgtaccgtt
     9661 gatcgggtta ttcggacgtt tttttgtatc tggtatttgg attttttcct ataaaaaagc
     9721 agatgcctat tatcagcggt tgtttccggg gaaatagaaa agaggctggg acagaagctt
     9781 ttaactccaa gaaataagcc gaaatttcac aaaaatttaa gaagcaattt tcgtaaattc
     9841 cttcttattt ctcgaagctg accacttctg tcacaactac tttcttactt cttacgttca
     9901 tatttcatat gatagattag taatagaagc aagaaccaca caggtgttag taataaagcg
     9961 attctcgtat cttttcctag gaacaagacg acacacacaa aggccatgta aagaaatacc
    10021 agataattac taaatgggta caacggcatt ttaaatgaat gagctttttt gccgaggata
    10081 cttttacgat atttcatgtg cgtgtagaca agcatactcc agataaacaa gaaacaagta
    10141 gtggcaatac tgcttactag tgtgaatact tcactaggca tcacgtaatt caagataaca
    10201 gaaatgaaaa tgacgatagt agaaaatgtc aaagcattcc ctggcacatg atgtgttgtt
    10261 aactttttga attttttagg ggcactgcct tcttgcgcta aagaacgaag catacgtcct
    10321 gtactatata ttgcactgtt gcaggcagaa gctgctgcac ttaatacgac taaattgatc
    10381 aaggaagcag caatcgtgat cccaatttcc gaaaatactt ctacaaatgg actgttatct
    10441 ggattcaagc tgttccatgg atagattgcc ataatgacaa aaagagatcc taagtaaaat
    10501 aagatgatcc tgataggaat attattgata gcttccggca acactttttc aggattttct
    10561 gtttctccag cgactaatcc aactaattct atcccagcaa aagcaaaggt taccatttgg
    10621 aaagctaaga taaatccttt tgcaccagtt ggaaagaatc cgccatgaga ccatagattc
    10681 gtgatactgg ctgtgccaat atctgtcgta tagtgggtga ggatcatata agctcctacg
    10741 aggataaagg caatgatcgc tacaactttg atcaatgcaa accaaaactc taactcacca
    10801 aacaatgaga cagccaccat attcaagcaa agaagaagac ctaatgcaat cagttcagga
    10861 agccactgag gaacaccagg cgcccagtag cgtacataca ttccgatggc tgttaaatcg
    10921 gccattgcaa tcgataccca gcagaaccaa tacgtccagc cagtgatgaa agccgctttt
    10981 ttgccaagat attctgcaac aaaatccaga aaagaatggt tattggtgtt tgataacaga
    11041 agttcgccta acgcacgcat aatgaaaaaa cagatgcatc cagtaataag ataggcgagc
    11101 aatatcgaag ggccagctag ctggatagac tttccggaac ctaaaaacaa ccccgttcca
    11161 atcgctccgc caatagaaat cagttggaca tgtctatttt tcagtccacg ctgcagttct
    11221 tgctcttcat tcatttttaa atcatcctaa ctaaaaaagt ttaattactt ccatattaaa
    11281 agagttttct gaaaaacaca actaagattc caaaattttc agaaatttac cttttatgag
    11341 taattcataa aaaagaactg cggtaaacac cgcagctctt tgtcccaatc agctattgat
    11401 cctaagcatt taaaatacgt gaaaggaatt cttttgtacg ttcttctttc ggatggacaa
    11461 agatatcttc tggtgtacct tcttctgcaa tcacaccttt atccataaaa atgacacggt
    11521 cagatacatc ttttgcaaat tccatctcat gcgtcacgat aagcattgtc aagccagtat
    11581 gtgctaagtc tttgatcgta ttaagcactt cgccaaccat ttcagggtct aacgccgaag
    11641 taggttcgtc gaatagcaat acatctggat tcattgataa tgcccgagca attgcgacac
    11701 gctgtttctg tccaccagat aattgtgctg gtttggcttc gatataacga tccataccaa
    11761 ctttttccag attttcgatt gctacttttt tggcagtttc tttatctcgt ttcaggacag
    11821 ttgtttgacc agacatacag ttttccaaga cattcatatt gttgaacagg ttgaaggatt
    11881 ggaaaaccat acctagattc gttcgatatt taggtaaatt atatttagga gctaatacat
    11941 tttctccatt ataaatgatc tcgccgcctg ttggtttttc cagtaaattg atacagcgca
    12001 agaaagtaga tttaccagaa ccggaagaac cgatgatcgt cacaacttca cctttattga
    12061 ctgttacgct gatatctttc aatacttcat tttcaccgaa gctttttctt agatgtttga
    12121 tttcaataat tttttcattc atgattcttc ctccttattc ctgcaaattt gaattgtcga
    12181 tttcttcaag cttggtataa gctgaaggac catcgatttt cttttctacc gcccgcagga
    12241 ttctggtgat cgtaaaggtc atgactaagt agatgatccc gacgatcgta aatgtttgga
    12301 agaattggaa attcgcaccg gaagcagagt ttccttggaa gaacaaatca gctacgccga
    12361 ttacacttaa aactgctgta tctttgatat tgataacaaa ctcatttcct gttgctggca
    12421 agatattccg taaaacttgt ggaataacaa cttttctcat cgtttggcca tgggtcatac
    12481 caatggcttg cgcagcttcg aactgccctt catctactgc aaaaatcccc ccacgaacga
    12541 tctctgtcat gtaagctcca gtattgagag agacaataaa caaagcggca atcgttcggt
    12601 tcaatgaaat gccaaaagcg agtgccagac cgtagaagat gaccattgct tgtaccatca
    12661 tcggtgttcc gcggaatact tcgacataga tagaaagaag tgcatttccg attttttgga
    12721 agaagcgagt cagtgcattt cctgattcag gtaccgaacg gaagacacca atcagtaatc
    12781 caagtgtagt accgacaact gtcccgatca atgcgatgaa caaggtaagt ccagcacctc
    12841 gtaagaacat attgccgtat tgtttccaaa tcgtagtgaa atcagaaatg atgccaggtt
    12901 tttcttcact atcagttgca gcaggctgat ctttgactgc ttggtccata attttggttc
    12961 gttcatcgtt agagatgcca gaaagaatct ggtttacttt ttggatatcc gggtcgcctt
    13021 tacgcatacc aacagaaatc tgaacatctt ccgggtttgt ctggaagcca tctgcaccgc
    13081 tgaattcaag catttccaag tctttgttca cactagttgc cgttacacct tccggacgtt
    13141 cgctgacata accatcgatc attccagaag ctaaagctgt tctcattgct gagaaattat
    13201 ccattgcttg ttgtttctgc acatctggta tctgatcgat cacagtatag tggaatgtat
    13261 ttaactgagc agtaatcttt gctccagaaa gatctgctaa actttttgct tttgcatatt
    13321 tgctatcttt ttgaactact actactaatt gtgattcgta ataaggatcg gtaaaatcaa
    13381 tttcttttcg tcgttcagca gtagggctca ttccggcgat gatcgcgtcg atcttgcctg
    13441 attgaagggc aggagccaga ccatcccatt tcgtttggac gatgactaat tttttcccta
    13501 aaccatctgc tactttttta gcgatttgaa catcataacc gccggcgtaa ctattttggc
    13561 cttgaattgg caaggcatta ttctcatctg tttgttgaga ccagttgaag ggtgcatacc
    13621 cagcctccat acctacgcga aactcatcgc tcggtgtttt ttcatctgca tttgccagag
    13681 atcccacact gaatagtgag aagacaaaag caaatagtac ggcaactgaa accatatttt
    13741 ttttcatttt tttctccctt tctgattata tgatacacga gcggtctaga tgaaatgcgg
    13801 taaagcttta tctgaaaaaa taaaaagcga ccgatttata gatataaaaa ggccgctaaa
    13861 attccataag gtgataaacc tctcacaaaa catagcgcaa cttagcatca ctgctaagac
    13921 agtccgcaag ctcttaactt gcgtcccaac atacactcca agcaaaaaag tgcacatttc
    13981 ggcgatcctc cctattcctg cttctccatg tttgcttcac tccacagggt taatgattct
    14041 cgcgacctct acctcattga ttgaggcatc tcgtattcag ttatttatac aaaaaaagtt
    14101 tacgatactc gagaggcttt gtcaatctat aactgctgtt ttttgaaaaa atactgataa
    14161 atatagaaat ctttactttt accgaaaaaa caatcaaaat gtgaatcatc tattcattgc
    14221 aaacgatagt ataattatac acaaaaatgt atgtaataca ataaaaaaag aactagaaca
    14281 gaaaatttcc tgtgtccagt tccatctcga tcatttaagc tgccgctttc tttggctcac
    14341 gtcctaggat cgcttgcaaa agaacaacga cgccataaat ggctaatatt agataaatca
    14401 aacttgagta ttgggtgatt ccccagcccc atgttcgata tacggctaat gtaatggcta
    14461 aaacaattgc ggcaatcacg ttgaataaag caaaagccca aagatttcca ttttctttgc
    14521 gtacaagata aaaaacagag aaataaacgc ttaacgctaa ccaggcgata caagccagaa
    14581 taacgcccca ataaataaag aatggtaaca tatcgattca cctcactttt atacagcagt
    14641 attgtaacac gttttcattc ttttgtcagt gaacattgtg ataaattgtt caaatgccca
    14701 taacactttt ttattcccat ttttctgact tttcaacaat aaatttgttc gagtatgggc
    14761 tataatggaa agtgaggtga caaaacatga aacaattaac agtagacgaa gcagcaaaag
    14821 agatattagc ttatcagcaa attacatttt tgaccggtgc aggggtatcc acaccttctg
    14881 gagtgccaga ctatcgatct ttgtcaggaa tctaccacgg gatggagcag ccggaatact
    14941 tgctgagtca tcaagcaatg gtgaatgaac cggataaatt ctatcaattt gtcaaaaaga
    15001 tttatcatcc tgaagcaaaa cccaatatca ttcatttgga aatggcacat ctagcgaaag
    15061 aaaaaaatgt ttgggttgtt tcccaaaaca tcgacggttt gcacgcaaag gctaaaagcc
    15121 cgcaactcgt caattttcat ggaagtttgt atcaatgtca ttgtcgaaaa tgtcaacaaa
    15181 cggtagactg gaaagaatat cttcacagcg acaaacatca agcatgcgga ggacaaatac
    15241 gcccagacat cgtgttatat ggtgaagggt ttcaagatgg agtcatggag caggcggctt
    15301 atgccgtcag ccaagcagag ctgatcgtca ttgttggaac gagttttcaa gttcatccgt
    15361 tttgtgatct ggtacagttt cgccagccgt cagccaaaat cttagtaatc aatcaaacgc
    15421 ctgtttattt agcggagcct tatacgttta ttcaagaaga cggagcggtt gttttcaaaa
    15481 aaatacaaga attaggagca gattatgact gaattcaaaa ccgaaaaaga aaaaatgatt
    15541 gcaggcgagc tttattttgc caatgatcct gagctggttg ccgaccgtat gtttgcaaga
    15601 agtcaaagcc aaatcatcaa ccaagcagaa tcagcggaac tgcgatccca attgttgaaa
    15661 gagacttttg gccgtacagg gaaaaaaatc tatatggagc cagttatcaa ttttgattac
    15721 ggatataata tatttgttgg ggagaatttt tatgcaaatt tcaattgtac atttttagat
    15781 gtcagcacga ttgaaatcgg tgataactgc atgttcgctc cgaatgtcca gctttacacg
    15841 gcgacgcatc cactacatcc agtgaaacgt aacagcggtc tggagtacgc aaaacccatt
    15901 aaaatcggta ataatgtttg gttaggcgga ggagttatcg tcacgccggg tgtcactcta
    15961 ggggacaacg tagtagtcgg cgcaggttcg gttgtgacca aatcttttcc cgacaatgta
    16021 gtgattgcag gaaatcctgc tcgtatcatc aaggcagtag aggaagaact aacagaagag
    16081 tctcttgaaa cattacgtca taagatcgat atgatcgatc gagagttggt tgctttatta
    16141 gaaaaaagaa tggatacggt aactaaaata ggacaagtga aaaaagaaga aaaccaagca
    16201 gtttatgatg caaaaagaga gcagcaagta ttggataaag ttgttagtct gctgaaaaat
    16261 aagggatata aggaaacaat aacagatacg tatgttgatt tgatgaaaca ctcgcgcgaa
    16321 taccaaaaca aaatgaagga ggaataagtg tgatcgaagg agagacacgg cgaaaacaaa
    16381 tcatgcaaac cttggcaaat acagaaaaac cagtgagtgc tagcaagttt gcgaaaactt
    16441 ttggagtcag ccgtcagatc attgtaggcg atattgcttt actacgagct gcaggtaaaa
    16501 gtatcgtggc aacagcaaga ggttatatcc tcgaaacaga agaaaccaat actgggcaca
    16561 tcagtaaaat cgctgtccag catggaaaag atcaaacaga agaagaactg agactaatcg
    16621 ttgaaaatgg tggtgaaatc attgatgtaa ttgtggaaca tcccctttac ggtgagttag
    16681 caggaacttt gcacatcaaa acgccacaag atatcgattc ctttatgaga cgttatcaaa
    16741 aaagtaatgc tactttgtta tccgaattaa caggcggcat ccatctgcat acgatccgct
    16801 acccagaaaa gcatatgctg aaaaacatca aacaaaaact agctgaagca ggaatcctct
    16861 acgaaggata acatcgtagc tgctactttt tatcctagaa actattctat catctctgag
    16921 ataaacaccc atatggaaga aataagaaaa aataagaaaa gatgcgtatc caatacacag
    16981 aattttttgg aattgcgatt tctagatgtc tatctaaatt tcgattagtg gagaaacata
    17041 aaaaagggaa gtgcagcttt tgagcactcc ccttttttat aaagctttct tgtagcatag
    17101 tatcgaagga acctgccaat tctctactcg atcaccaata cgccttcttt tagtgcaaat
    17161 ggattttgat cgttgatgtg gtcatagaac atcacaccgt tgatatggtc gatttcatgt
    17221 tgaacgacga tcgattcata attcttcaaa cgaatcttgt gtttttcacc attcatatca
    17281 taataagaca cagtgatttt ggcatggcga acaacatatc ctggtacttc tcgatcaaca
    17341 gataaacagc cttcaccttc ccctaaacaa gcatcttgaa cagaatggct caaaatttta
    17401 ggattataca taacagtact tagagaaggt ccatctgcct ctgggtcagg acttggtacg
    17461 tgaacagcaa tgattctttt agaaatatct aattgaggag cagctaagcc aacaccgcct
    17521 cgtaagtgta gttcttcagc tttgattgga tcttggctgt tttttaagaa ctcaagcatc
    17581 tcttttccta aagagatatc ttcttcgctt aatggaagag aaacttcttt tgcaacttcg
    17641 cgtaaggtcg gatttccttc acgaatgata tcatccattg taatcatgga atatgtaccc
    17701 ctttcttaaa agactgtgtt tcaagtctat tttcgcatta tagtttacca caaaaatgga
    17761 aaagtagtag taagattgtt tgaaaactac cttttcttaa tgattaacta cttgattttc
    17821 aacccggttt caggtaaagt atagctgtgt gctctaggca catgacctgc cacttggtta
    17881 agcgaatcac caacgtttta ctcagtcgca ggggaataat cgaaagaggt gaaagaaatg
    17941 gcaatttcaa aagaacgcaa gaatgaaatc atcaacgaat acgctcgcca tgaaggggat
    18001 actggttctc cagaagtaca aatcgctgta ttaactgaag aaatcaacca cttgaatgaa
    18061 catgcacgcg tgcacaaaaa agaccatcat tcataccgcg gcctaatgaa aaaagttggt
    18121 caccgccgta acttgttagc ttacttacgt aaaactgacg ttcaacgtta ccgcgaattg
    18181 atcaaacgtt taggcttgcg tcgttaattt tagtcaaaaa gagtgggatt cacatgaatc
    18241 tcactttttt tactcgtata tcaattaagt aaaattccgt gatcaagcta gatggactcc
    18301 tactaaccaa atgaaagctg ctcaaaagac ggctttcggc agttttcatt tgtttagtag
    18361 cgtcttgctt tcacgagaag gaaaaggaga agttatgtca gaaaaacaag tattcaaaac
    18421 gacatggggc ggccgtccct tacaaatcga agtcggtcag ctagcaaaac aagcaaacgg
    18481 agccgtttta gtccgttatg gagatacagt agtcttaagt gcagctgtgg cctctaaaga
    18541 agcaaaagat acagactttt tcccattaac gatcaattat gaagaaaaaa tgtatgcagc
    18601 agggaaaatc cctggaggtt tcatcaaacg agaaggccgt ccaagtacgg aagcaacatt
    18661 gactgcccgt ttgatcgacc gtccaattcg cccgatgttt gcagaaggct tccgtaatga
    18721 agtccaagta acgaacatcg tgatgagtgt agaaacagac tgctcgccag ctatggcagc
    18781 aatgctaggt tcttctttag cactatcaat ctcagatatc ccatttgacg gcccaatcgc
    18841 aggcgtagaa gttggtcgtg taaatggcga gtacgtcttg aatccaacag tagaacaagc
    18901 agaacaaaca gatatcgaat tgactgttgc cggaacgaaa caagccatca atatggttga
    18961 aagcggcgcg aaagaagttt cagaagaaga catgcttggt gcattgctgt ttggttttga
    19021 cgctatcaaa gagttagttg ctttccaaga agaaatcgtt caagcagtag ggaaagaaaa
    19081 aatggaagtg actttactgc aagttgatga agtattgaaa aaagaaatct ttgatgcttc
    19141 ttacgctaca atgaaagcag cagtaatgac tgaagaaaaa cttgctcgcg aagataatat
    19201 cgagcaggtg aagatcgata tccgcgaagc ttatgctgag aaatttgcag gtcatgaaga
    19261 tgaagatcac ttgttgaaag aagttaaaca aatcacagaa gatcttgaaa aagatgtcgt
    19321 acgtgaattg atcaccatcg ataagatccg tcctgacggg cgtaaattgg atgaaatccg
    19381 tccattgtca tcagaggtca gcttattgcc acgtgtacat ggatcaggtt tgttcacacg
    19441 aggacaaaca caagctttat cagcttgtac actcgcacct ctaggagagc accaaatcat
    19501 tgatggttta ggggtggaag tcagcaaacg attcatccac cactacaatt tccctcaatt
    19561 ttctgttggt tctacaggac gtgcaggctc tccagggcgt cgggaaatcg gacatggcgc
    19621 attaggtgaa cgtgcgttag cacaagtaat cccttcagaa gaagaatttc cttatacgat
    19681 ccgtttagta gcagaagtat tggaatcaaa tggttcttct tcccaagcaa gtatctgtgc
    19741 tggcacatta gcattgatgg atgcaggtgt accaatcaaa gcgccagtcg ccggtattgc
    19801 gatgggactt gtttctgatg gtgaaaacta tacgatccta actgatatcc aagggctgga
    19861 agatcatctt ggcgatatgg actttaaagt tgccggtaca aaagatggta tcactgcttt
    19921 acagatggat atcaagattc aaggaatcac agaacaaatc ctaactgaag cattaactca
    19981 agcaaaacaa gcacgtatgg aaatcttaga agaattgact tcaacgattg ctgctccacg
    20041 tgaagaatta agccaatatg caccaaaaat cgaaatgatc cagatcgaac ctgctaaaat
    20101 caaagatgtt atcggtaaag gcggcgatac gatcaacgga atcatcgatg aaactggtgt
    20161 gaaaatcgat atcgatcaag atggaaaagt aagtattgct tcagctgatg cagaaatgat
    20221 caaaaaagct atcaagatca tcgaagattt gaccaaagaa gtcaaagttg gtgaagttta
    20281 tctagggaaa gttgtccgta tcgaaaaatt cggtgcattc gtcaacttga tcaaaggaaa
    20341 agatggactt gtccatattt cccaattagc aaacgatcgt gtcaataaag tcgaagatgt
    20401 tgttaaacta ggtgatgaga tccttgtgaa agtaactgaa atcgataaac aaggacgcgt
    20461 aaatctgtca agaaaagcaa tgttgaacga agatggttca acgaaaaatg aagaaaaata
    20521 atttaattca taggaataat gtacttgtga tcgtgaaggt gacaggggga caataattat
    20581 tcgaaaaaag gggactgtga caaaagccgt gtcacagtcc ttttttttcg aataaacggt
    20641 gttcaaaagc ttgcatctgc ggtaataagt ccagccaagt taaaaacatt aggagctgtt
    20701 tttacttggc tgttcttatt acttgatgca gaacagcttt tttacaacct ctccaataaa
    20761 cgatgttcaa tcagctgtcc ctcgatatta gctcaaaaaa cggcaaaata tgaggaacta
    20821 tttttccgtt tgtttctcta atactcaggt caaaccgctg atttcacaac ctctgattca
    20881 cggtgttcca gaagcagctt ctacggaaat aagctgaaca cttttgttac aacttctttt
    20941 tttctggagt ttcttggagc atatttttgg agtccttctg aataaagcgt aaaataaaag
    21001 tgagaaatat ggataaagga gggaaaggag atgctgaatt ttttatggtc gttatttatc
    21061 ggcggtatat taggtttgat agctggcgca attttaggaa aggaagtccc gggaggcgta
    21121 tctggaaata tcattatcgg ttttttaggt agttggctcg gcgaattttt actaggacca
    21181 ctgggaccgg aactcggcgg tttttatttg ataccagcat tattgggtgc aattttttgc
    21241 cttgctcttt attcatttgt ggcaagacgt atcagagaac gtccaaagaa agaataattt
    21301 tattcaaata agttgacact ttagtgtgtt acttggataa ttagaagcaa tttaggaaag
    21361 cgggtgtcat ggataagaat atcatctatc attcaagctt acctaaagga tatccatata
    21421 acaagaaatg agggaaaaac gtggcaacaa cgcacaatac caaaaagata tcaatggctg
    21481 gcctgcttgt agcgatggga gtcgtctacg gagacatcgg gacaagtccg ctttacgtta
    21541 tgaaatcgat tgttgaagat aatggcggat tgtcaggaat caatcctgat tttgttatcg
    21601 gatcagtctc attgattttt tggacattga ctctactgac aacaatcaaa tatgtcgtga
    21661 ttgcattgaa tgcagataac catggtgaag gtggaatttt ctcactctac acacttgtcc
    21721 gaaaaggtgg gaaatatttg atcattcctg caatgatcgg aggagctgcc ttactagcag
    21781 atggggtatt gacccctgct gtgacggtaa cgactgcgat tgaaggcttg cgaggaattc
    21841 cgcagttttt tgaccggttc ggtaataacc agaacgtgat cgtcattatc actttagtta
    21901 ttatttttgt tttattcatg gtccaacgct ttggtacaga tattgtcgga aaagcattcg
    21961 gaccaatcat gttcgcttgg tttacttttt taggcgtaat gggattaatg aacttcagtc
    22021 aagactggac agttatccgt gcactgaatc cttattatgc tatccagctg ctttttagtg
    22081 cagataacaa attaggcttg ttcattcttg gaaatatttt cctggctacg acaggtgcag
    22141 aggcgcttta ttccgatctt ggacatgtcg gcaaacgaaa tattcgtctg agctggcctt
    22201 acgtcaaaat ttgtttggtt atcaattatt ttgggcaggc tgcgtggttg ttgaatgttt
    22261 atcaaaaccc tgaagcacag caaatcaaga acctgaatcc attcttccaa atgatgcctc
    22321 aaggttggat cgtgatcggc gttgcttttg caacagtcgc tgcagtcatt gcttcgcaag
    22381 cattgattac aggttcgttt acgctagttt cagaagcaat caagttgaaa ctgctgcctc
    22441 ggttaaagat catgtatcct gggaacagta tcgggcaaat gtatatccca gcagtcaatc
    22501 tgattttatg gatcgcctgt tctctagtgg ttgtcacatt ccgaacgtct catcatatgg
    22561 aagctgctta cggtctatcg atcactgtga cgatgttgat gacaacagca ctgctttact
    22621 tttatcttat ccaaaatggg cattctcgtt ggcttgcata tttagttacc tttttcttcg
    22681 gtgcgataga agtcgttttc tttatctcaa gtgttgtcaa attcttccga ggcggttttg
    22741 tagccgttct gattggtttg atcatcttag tcgtgatgtt tatttgggaa caagggaaca
    22801 ttattcgcga atcgtcaacc gaggatgtgt ctttgagaga ttatatcccg cagcttagag
    22861 aattacgtga tgatacttct ttaccaatct atcagacaaa tgtggtcttc ttggttcctg
    22921 atatggctga agataaagtt ggccgacagt tcatctattc gattttagat aaacggccta
    22981 aaagagcacg agtctattgg tttgttcatg tggaagtgac ggatgagcca tatacgaaag
    23041 aatatcaagt agatatgatg gacactgatt ttattgtcca agtgaatctg ttcttaggct
    23101 tcagggtcca acaggaaatc aacgtatatg ttcgccaagt catccatgat ttgatgaaac
    23161 aaggccgttt gccaaaacag ccacaaagat actctctgac accagggcga gaagttggcg
    23221 atttccaatt tgtcctgatt gatgaagtag tttccaatgt gacaactcta ggaaaatggc
    23281 aacgtcaaat catgcaggca aaactggcaa tcaaaaaaat cgctacctcg ccagaaacat
    23341 ggtttgggct agagtacagc gaagtaaaac atgaaactgt tccgcttatc attggaaata
    23401 ctcggaaaac atggttgaaa gagcggttgt gaaagtagcg ctttgacctg aactaatgtc
    23461 gaatgtcagc tacttgaaca ccgtttatca gaggtcatga aaaagctgta ctgcaacaaa
    23521 cagtaagaac gagcagataa aaacagcttc tcatgttttt catctgtttg ggcttaatgc
    23581 cgcagatgca agcttttgaa taccgttttt atcagaggtc atgaaaaagc tgtttagcaa
    23641 caagtaacgc aatgattttg aagtgataag gagatgtggc tgaaaaatgc cgcatctcct
    23701 tttttattcc taaatatgaa tacgcattat tttcaatctt tttgtagaaa aagggtacaa
    23761 tagaaaaaga gaaaggcggg agaatatggt tatcaatttc attattttat ttttgatgat
    23821 tgcaattact tcattttttg tagcaagtga atttgcttta gtgaaaatca gacgatcaag
    23881 attggaacaa ctagaaaagg aaaatgtaaa gaacgccaag ctagctcttc atgttacaca
    23941 ccatttagat aactatttat cagctagtca attaggaatt acgctaaccg gtttgatcat
    24001 cgggtgggtc ggtgaaggat ctgtcgcggc tttactagaa ccagtgatcg gtaaactacc
    24061 ttttagttcg gctattagca gtacaatctc tgtggcgctg ggatttattt tagttacgta
    24121 tatcgatgtg gttattggcg aactgcttcc taagagctat agtatcgtca atacggagaa
    24181 ggtcgtcttg ttcgtagtta aaccacttca ttatttttat aaagtaatgt ttccttttat
    24241 ttgggtattg aatcattcag ctgcagggct agggaagcta ttaggtgtgc gtctcgtatc
    24301 tgaaggagaa gagacattgt ctcaagaaga gctgacatta gtcgcattga attcttatga
    24361 aaaaggagag ttgaccaaag aagaatatca ttatttggaa aacgtgtttg aatttgatga
    24421 taccttggca aaagatatac aagtagaccg tacgtccatg caagtattcg aagcttcaga
    24481 aaccgtcaaa gaagcgataa aagcttcgat tgaacaagga catactcgct atccggttat
    24541 cttagaatca aaagacaatg ttttaggtta tgtcaccttg ccagatttga tcaagcaatc
    24601 ttataaagat gaccagctga cagtagaaca gttgattgaa gagccgatcg taacaactga
    24661 aacgatccct ataaaaaaat tattgacgat catgcgaaaa aagggaaagc atattgctat
    24721 cttaaaagat gaatacggag ggacaagcgg cttagtgacg attgaagata ttttagaaga
    24781 aatcgtcggg gaaatccgag atgagacaga tttggacgaa gcattgatag cggaacagtc
    24841 agatggctct tatattatct ccggcaaact aacattagat gatttccagc gttattttca
    24901 tgtagagatt ccagagtttg aagaaacgaa ttttactaca cttgcgggct ttgcttctag
    24961 ccgatataaa gaaataaaag caggaacaat cattgagatt gcatcattcc gtttcacggt
    25021 attagaatac cagcatgcac atatcgatta tttcaaagta gagtccacgg aaagaaaaac
    25081 agaataaaag aaaaaaggct gtgacatttt ttgtcacagc ctttttccta cgattagtca
    25141 agtaaaaggt gcaagaaaaa tagctcgtaa acgaaagcga caaaatcaaa gcacaccaaa
    25201 taaagccccg ttcgtaaaaa attttcgatc tgggcaaaga agtggtatac tgtaagtggt
    25261 ttagttaatg gtccactgag accgcgtacg catagtcgta ctctgtatgt gccctaacca
    25321 tagcgtgcat acatttcaga agcttgttca tacaagcaac tgtggcaacc ttatcttt
//
LOCUS       DAFEPQ010000036        25185 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.036, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000036
VERSION     DAFEPQ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.036"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(165..692)
                     /gene="msrA"
                     /locus_tag="LP543_001601"
     CDS             complement(165..692)
                     /gene="msrA"
                     /locus_tag="LP543_001601"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL2321770.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(776..1453)
                     /gene="pgmB"
                     /locus_tag="LP543_001602"
     CDS             complement(776..1453)
                     /gene="pgmB"
                     /locus_tag="LP543_001602"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBL2321771.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(1450..2580)
                     /locus_tag="LP543_001603"
     CDS             complement(1450..2580)
                     /locus_tag="LP543_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL2321772.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(2580..4763)
                     /locus_tag="LP543_001604"
     CDS             complement(2580..4763)
                     /locus_tag="LP543_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBL2321773.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(4760..5785)
                     /locus_tag="LP543_001605"
     CDS             complement(4760..5785)
                     /locus_tag="LP543_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL2321774.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(5830..6657)
                     /locus_tag="LP543_001606"
     CDS             complement(5830..6657)
                     /locus_tag="LP543_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBL2321775.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(6654..7721)
                     /locus_tag="LP543_001607"
     CDS             complement(6654..7721)
                     /locus_tag="LP543_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBL2321776.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(7747..8583)
                     /locus_tag="LP543_001608"
     CDS             complement(7747..8583)
                     /locus_tag="LP543_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2321777.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(8580..9449)
                     /locus_tag="LP543_001609"
     CDS             complement(8580..9449)
                     /locus_tag="LP543_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2321778.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(9519..10802)
                     /locus_tag="LP543_001610"
     CDS             complement(9519..10802)
                     /locus_tag="LP543_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBL2321779.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(10816..12501)
                     /locus_tag="LP543_001611"
     CDS             complement(10816..12501)
                     /locus_tag="LP543_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBL2321780.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            12776..13801
                     /locus_tag="LP543_001612"
     CDS             12776..13801
                     /locus_tag="LP543_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2321781.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(14396..18070)
                     /gene="rpoC"
                     /locus_tag="LP543_001613"
     CDS             complement(14396..18070)
                     /gene="rpoC"
                     /locus_tag="LP543_001613"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBL2321782.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKSVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(18128..21745)
                     /gene="rpoB"
                     /locus_tag="LP543_001614"
     CDS             complement(18128..21745)
                     /gene="rpoB"
                     /locus_tag="LP543_001614"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBL2321783.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            22473..23441
                     /locus_tag="LP543_001615"
     CDS             22473..23441
                     /locus_tag="LP543_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL2321784.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            23459..24364
                     /locus_tag="LP543_001616"
     CDS             23459..24364
                     /locus_tag="LP543_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2321785.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            24361..25167
                     /locus_tag="LP543_001617"
     CDS             24361..25167
                     /locus_tag="LP543_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2321786.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
BASE COUNT         7034 a         5493 c         4719 g         7939 t
ORIGIN      
        1 atatatagtg ttctcttacc aaatccttgt gacttggagc taggcgacag gttcacaacc
       61 tctccactaa atggtgttca atcagctgac cctcagtatt agctcaaaat gctggtttta
      121 caacctctga tatacggtgt tccataagta gctttttcgg cttcttattt cttgacgccc
      181 caatgtcgac gaaggaaacc tgcacgtcca gaaccttctc gataaagatt gtaatggagc
      241 ttgtttttct tgtaataatc ctgatggtaa tcttctgcag gataaaattc gcttcttggt
      301 tcgatttttg tcacgattgg cttatcgaat cgcccgctgt cttgcaatgc ttgttttgaa
      361 cgctctgcaa tatttcgttc ttcttcatca ttatagtaga tcactggacg ataagaatca
      421 ccgcgatcag cgaactgtcc taatgcgtca gtcggatcgg tttgctgcca atagatttcc
      481 accaactgat cataagaaat aacttctgga tcatattcga tctctactgc ttctgtatgc
      541 cctgttgtgc ctgttgttac ttgttcataa gttggatttg gtacatgccc gccagtataa
      601 ccggatgtca cagaaatgat ccctggctgc tcttcaaatg gtttgaccat acaccaaaag
      661 catccgcctg cgaaaactgc tttttttgtc atataaaatc cccctttttt ctcatcatac
      721 cttgatttcc aaaaggagga taacgttttg cttgtgacag aaaaaacgga aactattata
      781 tacgtgatac ggcttctgat aaatgccaaa acgaacgtac ttgatcaaaa acggttaaat
      841 caggaatcac atattcagca gaaggaagat ttatgttttc acctattccc aatacatgca
      901 tgttaccagc caaagctgct tgacaaccag cttctgaatc ttcaatgacc aagcatgcat
      961 ttggcggtac attcaattgc tgtgctcctt tgagaaaaac ttctggatca ggttttgctt
     1021 ttgatacttg cgtgccgtca ataaggacat caaaataact tgtcaaattc gttttttcta
     1081 aaatgaggcg tgcatttttg cttgctgatc ccaatccgat cttgatgttt gtttttttca
     1141 agtactcaag aacagctatt gcaccaggca aaacggatgt ttcatccatt ttttgaatcg
     1201 cttgaagata tagattgttt ttttgctcag caaaggctga tttctctttt tctgagaatt
     1261 tctgttccat ttttccaaat gacagcaatc gctctagtga ctcggttctg gatattcctt
     1321 tcagttgctc gttttctttc tcagtgaatg gaatggacag ctggttagct aaaaccagcc
     1381 acgcttcgta atgataacga gctgtgtcta caagaacgcc atctaaatcg aatagtgctg
     1441 ctttgatttt catcgattac ctccttgaaa atgaacgact tgaagtaaag atgcgatgtt
     1501 tttagctgtg cgatgaagat tatcagtggc ttgctgtaaa gcttcttgta aaggcacagc
     1561 tgcaggaagg atcggaaaaa cggccgaaat gccaacatct gctgcttttt cgcttccttt
     1621 accaacactt ccgcaaatag ctatcacagg aacgccctct ggtactcttc tagcaatccc
     1681 aactggcgct tttcctttta gactttgtcc atctagacgt ccttcgccta cgattacgag
     1741 gtctgcttgc tgtacccgtt tatcgaactc cagtaaatcc aatacgtagt cgatgccaga
     1801 gactattttc cctgaagcaa atgctaacag tcctgcaccc attccaccac cagcactact
     1861 gcccgatgcc tcgattactt gaggggcaaa tctcatataa aaatcatgca tggcttggtc
     1921 agccactgct aattcatttt ctttcaagcc tttttgtggt ccgaaaacaa aagaagcgcc
     1981 ttcttttccg cataaaagat tagtaacatc agtaatgaca gtgattttgg ctttaatcag
     2041 ctcaataggc acgttcgttt tatcaactgt agcaatttta cctagatttt ctcccagtgc
     2101 ttctactttt tgattatctt gatcgtaaaa agcataacct agaccttctg ccatgccgat
     2161 gccgccatcg ttggtactac ttccgccaac gccgatgatg atgtgtcgaa caccttgttg
     2221 caacaattgt cgtatcagtt ctcctactcc tcgcgtggat aacctaagtg gctgttgttg
     2281 gtttagtggg acatctttca atccgacgat ttctgccatt tcaaaaatcg cagtttgacc
     2341 gtctgaagcg tactggattt ctttgttttt gccaaatgca ttagtaacga ctgctgtttg
     2401 ccgcttcaac cttaaactgt ctgctaatga tgaaaccgtt ccttctcctc catcgcctat
     2461 aggtagcaga tcatattcag cctgaacaaa aacttcttga aaacctgatt gaatggcttg
     2521 tgctgcctct aaggctgtca gactttcttt gaaagaatct ggcgcaatca gaatacgcat
     2581 tattttcccc tccgttccaa gatcagttct ttttccagct ggtattggtc tccattgaca
     2641 aaaataggta atgtttcttt cgtctctttt tttattacta tttgtttttt tgtcaaaaca
     2701 aatagcaacc tgtgattttt ccaaataaaa ggaaaagaaa ttttttccca tgctgaagga
     2761 agtcttggag aaatagaaag agttccattc gttactgtaa tacctgaaaa accaaatata
     2821 actgctagcc atagtgctcc taatgaagca gcatgaattc cgtcatctga tgaatgcgga
     2881 tctggtccta aatcaatcag gcacgcttct tggaataaat gatacgccca ttccatttct
     2941 tggatacggc aagcctctat ggcgtgaatc gctttactca acgatgagtc gtggattgtt
     3001 ttgttttcat agtaatgcaa attcttagaa acgattggtt gagaaaaata ctcaggtaat
     3061 aagtataaca gcatgacaag atctgcttgc ttgctgactt ggagttcatt tacttcgcta
     3121 cgggcataat caagtaaaat agactggctg ccaggacgtt ctttgtaagt ggtcaaatca
     3181 ataactggct tactgagata cgtgtcatct tgcggaatca gctgttcttt gttaggcact
     3241 ggcaaatata acttatctaa aaaattttga cattctttca aaaaaagcgg atcgccagct
     3301 tgatatttaa cagctaaggc cacattataa tgcgccagat agttcgtata tgcattattg
     3361 tcgatatatt ctgtgtattc atctggacca atgactctgt gtatctctaa atgatcatcg
     3421 acttttacag ctctgctcag ccaaaaagct gctgtttcat gaatgagcgt ttttcccttt
     3481 gtttctaaaa acgattgatc ctctgttgct tggacatact cttgaacggc ataagcaagg
     3541 tctgcactga tatgatgttc tgcggcagct gaagcaactt cttgtcttgt tccggttttg
     3601 atattcaatg cagcaaattt gggtgtctct tcctgaccag tgtgtgcgct ttcccatggg
     3661 aaaagtgcac cttgatagtg ataatctctt gctttttgcc gtgcgccttc taatcgatca
     3721 taacggtaca gcagcaaatt ctttgctact tcaggaaact gatataaaaa gaaaggcagg
     3781 ataaaaattt ccgtatccca gaaaacatgc cctttataac cttcgccagt tagtcccttc
     3841 gcccctacac taacacttgg atcattgcgg ggagtcataa tctgtaaatg gtaagcagcg
     3901 aagtccatcg ctaactgatc gaaagaagaa tcgccttgga tcgttatccg atgctgatgc
     3961 caaaaatctg tccaataaga catcgactgc tctaaaattt ctgtataact aacgcttttc
     4021 tgggcttggg caatactttt cttaaacggg tctgtttgct gaagagaggt agagagcacg
     4081 atgatcttct ccagcgtaaa ggacgtattt tttctgatct tttctgaaaa ggaagcggtg
     4141 atccggcgat tttttgcttg gaatacacct gctttattga atttagctgc aatcccaata
     4201 tcaattttac tttctgttgt ctggtaggat gcactgatca aatcgtcacc aaatactcgc
     4261 agttcttttt caaccaactg ctgtgtacca aaattggttt gctgtccatc gatacccgtt
     4321 ttcacatcta cttggcagtc ttcggtcaac ggcttgatga ccactttcag ggctgcctga
     4381 tggagattat tttgatcagc aacacgacaa gtaatgactt ccactttttt tccttgagca
     4441 gtttcaatga gcgcatagcg tttaagctct cccatttcta aatctagtgc ccgctcgtat
     4501 gcaagaactt tcatcgtctc aagagaaaaa tgttcaccgt cgatttttat ttcccatctg
     4561 atgacatctg ggagattcac taactcggct gattctgctc ctaaaggtcg gttatataca
     4621 ccagctgcaa acattcccct tacttgattc cgatagtctt cctcctggct tcctctgata
     4681 ccaaaaaatc catttccaat ggtcatctga ctcgcatact tattgatata ctctaaatca
     4741 aactgttttt cttctacttt cataaggtaa taacttctcc tgtttctgcc gaacgatata
     4801 aattttccac cagattctgg atgacatatc cttgccagcc atctgcaatc atctgagtct
     4861 ggtcaccgag cgcccgattc acaaaatgag ccatacttag ttcatgtggg tcattaactg
     4921 gcgtggtctt ttgtaaggaa accagctttc catctacatc ttcgaatagt tcgcctggat
     4981 acagattagc acctgcctga tcgccacaaa aagccacatt cagtttgtct tctctgatat
     5041 tcaaagcaaa cgaagtattc agttggatga tcttgccgtt ttctaattcc agtgtgccaa
     5101 aaattgcatc ttctactgtg aatttgcttg gatcccattt cccgaatttc cctgaatttt
     5161 ttttcgtacc aattttttgg aagctgtacg cccgaacgct ttttacttta ggaaatccag
     5221 taatatacat cgctgtatca agcatgtgaa tgccataatc gatcaaaggc ccgcctcctt
     5281 gaagttcttt gtcgataaac gttccccagc ctggaacacc agaccgacga agcgcattcg
     5341 cttctacgta ataaatatcc ccgagaacat caagatgatt cttgatcatt gttgcttcgt
     5401 cagaaaaacg atgctggaat ccataagcca aaatctgccc gtttttatcg gctgtctctg
     5461 ccatctttct tgcttgatca gctgtcatag ctggtggttt ctcgcacatc actgaagcat
     5521 tagcttcgag ggcagccaat acatgttcga agtgaaagcg attgggtgta cagacactca
     5581 cgatatcggg agaaatctca ttcagcattt gtgtaactga tgtatacgca tgagaaaatc
     5641 ctgtacgtcg gcaaaagtct tctgctcgct ggctgactgg atcaaccaca gcggccaatt
     5701 ctaagacatc tgatcttttt tgataataag cagcatgtac tttttctgct acttgtccag
     5761 cgcctatgat tgctactttc ttcatttatt tctccctcct ttgcttattt acttattgtt
     5821 attctttctc tattctttct ctaaatcatt taaatgacgc gctgtttcca gacagttttt
     5881 caagtattgc aatgattcct gataaatttt cacttcattt tctcctcgca aacgacattc
     5941 atataccata taaccttcat actcattttg aagtagcgtt tgaaaatggc gagtaaaatc
     6001 aattgcgcca gatcctggtt ggtagcgatg attatctgct aaatggatat gacccacata
     6061 ctctttttgc gtagtcaatg cttttgaaat atcatcttct tcaatattca tatgataaaa
     6121 atcagcgatg atctgcacat gtttcaattg attagagtca atgtaatgac gcgcatccgc
     6181 taacaaattg atcatatggt cttggtatcg gttcaaaggc tccaaataaa taaccgtacc
     6241 tgtttcttcg gctactttat ctaaataacg aagtgattcg ctgactgctt tatagtctcc
     6301 ttctttgctt cggggtgaaa ccatcggcgg taatcgataa gtgaacattc cccaagctgc
     6361 tgggataacg attcctttgc ctccaacctc gcttagtgca cgcaaaatct cagcaatttg
     6421 acgtacacca ttaagacgcc gttcttcgat aaagtcaccg atccacccat cgtagccgtt
     6481 acaagctgtc actactggga tccctgtttc tttgattgcc tgtttgactg tgtcgatatt
     6541 ttctattaac aacttaccgt cgatttcgta ttcttcaaat ccacagtctt tgataaatct
     6601 aaatttttct aaaatgtctc ttgggaaaaa tgcttgatct tgcgtaccga atttcattgt
     6661 tctcctcctc ggacttgaac gcccattttg atactttctt ctgggtgctg gtctacataa
     6721 cgcatgtaag cttctgcgct ttctgcaaat ggcacaactg gatcaatgat ttcttcacaa
     6781 tccaagtaac cgttcatcaa taattcccaa catgtgtcct cgattcgttt acggttccat
     6841 cttgggtaat ctgggtttgg ttcactagat gctcgagcaa agacgatttt tgcattattg
     6901 aaatgtgctt cgcgaccaag attaaaccca gctggaaaag gtttagcaaa ggcaacataa
     6961 gaaatgatcc cgccatagcc gatccctctt aaagaagctt gtaatgcggc agcactgcca
     7021 ctcgtttcaa taactgaatc tactccacgt ttatctgtca attcctttag acgaaaaccg
     7081 acatctgctt tcataggatc tagcgtgtaa tcggcaccgt atttttctgc taattctcta
     7141 cgctgcgcta tgggatcgat cccaacgatg gtagtagcac ctgctttttt agctaattga
     7201 atcgcaattt gcccgatagc gcctaaaccg aagattgcta cagcatcacc tgctcgaaca
     7261 tgtgcttccc ggataccacc taaagcgaat tgagcaggat cgtaacatac tgcatttttc
     7321 cattgatcca accgatcaag tttacgtagt ttataattat caacaccgtt tgtaatcagc
     7381 gtttcacgaa tgggtccata agtgcagact aactctcctt tttgatactc tgtcaccaaa
     7441 ctgcccgttt cttcgatcgt accgacaatc atatttccta attgaaattc gccaaatacc
     7501 acaccgcgtt ggcttccttc ttctcttggc aagaataggc gccattcttc tgaaaattct
     7561 tcatcgataa atggacttaa tcctctaaag tcgacaattt ctgttcctgg ttttggagta
     7621 gcgaaaatta ctcggatacg tacttcgttc ttttcgatct cacggtcttc gtaatctttg
     7681 aatccggcta ttcttggttc taatgcaact aattttttca tcttttcttt cctctttctt
     7741 ttctttttat ccttttacgc cgccttcgac taaatcacct ttaataaatc gttcagataa
     7801 cgcatacata atgatgactg gtaaagcggt caccaatgaa gcggccatca ttcgtcccca
     7861 aatgtagtct ggtgtactga ataatgtgtt caaaccgatt ggtaaagtaa atttttcgga
     7921 ggtcgacagg aaaatcgaag caaataaata atcattccac gaaaccataa agcaataaac
     7981 gaagacagaa atgattccag aaatagccaa tggtataatg atccgaataa tgatctggat
     8041 ccggctaaga ccatcgatca ttgctgcttc ttcaatatca gtcggaatcg tgtcaaaata
     8101 actttttagc atgaatatcg cagtaggaag tgtttgaacg accatcgtga taatcagtgc
     8161 tgtattggta tcatacaaac ctagaccgga aatgatttta aataatggaa caacgatcaa
     8221 aataccggaa aacatataaa cggtgtaaaa gctagcattg attttcgagc gagctttgaa
     8281 gcgtagtttt gacaaagcgt atgccccgct gatcccaatc actactgcag ttacggaggc
     8341 tatcaaggag atcactaaac tatttttgaa atacatgatg aatggaaaaa tctcagcatt
     8401 gaaaatatcc tgataatgtt caaaagtcca ttctttcgga aaaatagttt gtgtcgttgc
     8461 aattgcttct tgtgaatcct tgaatgatgt catcagcatg atcaaaaatg gaaacaaatt
     8521 gacagctaat agcaacaaaa tcgtcgcgta aaataagatt ttcctgctct ttttattttt
     8581 catttcaagt ccaccttctt tcgtgagagc aagacgaccg ctgcaataat gatgaacaat
     8641 agaatcgata tagctgctgc ttttccaaga tcattgaacg caaaggctgt ctgatacaaa
     8701 taaacgccta agatattgac tttattcgtc agtagataga catctgtaaa catatagaac
     8761 atccagatag atcgtaaaac aatcacagtt gctagtactg gtttgattgc cggcagtgtc
     8821 acgatcttga acttctgcca agcatttgca ccgtccattt cagctgcttc ataaagcgtt
     8881 ttgtcgactg tttgcaaaat agccaaaaat gaaataaacg catacggata gtatctccag
     8941 atagcaaaca aaacgaccat aaagaagcta gcaactggct ggtcgaacca cagcggtgct
     9001 tcgttgaaaa gctgcaacct atctactaac agatcattga cgattccata actattattg
     9061 aacatatatt tccaagcaaa aatcaaagaa atagatggcg taacataact taataaaatc
     9121 agtgaacgca ccgttttcct aaatctgaac ttgcggttaa gcaaaatagc tacggctaat
     9181 cctaaagctg tactgcctgc tacaactaag aatgtgtaag caactgttaa acctaaagaa
     9241 cgataaaatt cactatcact taaaatttca agataattgc ttaatccgat aaaaacggcg
     9301 tctgagttag gattcagcgg catatcgaaa aaactgatct gaatattgga aagcattgga
     9361 atcagcacta aaacagctaa taaaagcatg cttggtgcta aaagtcccat ggctaatttt
     9421 aagtcagaat gtttccttgg acgattcatt gaaacctccc cctgataatc tgccaatagc
     9481 tatctacttt tttcgaacac cattggcaga acttcttgtt atttcgtatt tttttgtgcg
     9541 tcagctaaag cttgatcgac atcttcgttt ccgacagtta cttggttcac tgcttttccg
     9601 atcacacctg agcttgtgat atctcccatt ttcgtgaaat tcttttcccc gactagtcca
     9661 aagacttgta cttcatcaaa agcactagca atctcagatg gcagctcgcc aaaagacttg
     9721 atgacttcat tttctttgta accatccagt tcgactactt gtttattcac tggttgagca
     9781 ccaccaggag acatcaagac ccacttttcc atgtttttcg gttgagataa gaaagctgtg
     9841 aatgcttcag cagcctcttt ttgtttctcg tctagaccag aagaaatcgt caatccagat
     9901 actgtgccat agacagcttg atctttttgt gttgggatag caaaaccgat attttctgag
     9961 ttccccgctt cataaacaga aggcaaaatg taagttgaat agattgccat tggtgctgtt
    10021 ccattcatga aagcatcttt gatttctgtc acatcattag agcctggcat cgtgtattgg
    10081 gataagtttt gataaaacga caatgcttct ctcatctttt ccgtatcgat cgttacctcg
    10141 ccttcatcat ccaaaacatt tgctccattt gacaaagcaa attgagagaa cgcttgctca
    10201 ctcattgttc cttcagcagt aggcatagca attccatatt gtttattctc tttatctgtg
    10261 aaatgttgag caatttttaa aatatcatcc caattttctg gttctgaaaa accagcttct
    10321 gataattttt ctttgtcata ccaaatacct tgaacccagc cgctaattgg tgcagcaata
    10381 tagctttttc catcttctga acgaacaagg tttttggctc cgtcataata attatcttct
    10441 ccagcttcct cgataacgtt ttggactgcc gtctgatcaa tcagttcatc tttatccatc
    10501 actttagcaa aatcctgact gacttccatt acttctggta atttaccgga acgagctaac
    10561 gtaacgattt ttgtattgaa cgctgattct tctaccggca cttgcttgat tttgatgtct
    10621 ggattttctt tttcaaaatc agcaaccagt tcattgatca cacttaatcg ttcctgttcg
    10681 acagaagagt gcatgaattg aatcgttgtc tgttgtgatt cgttagagct ttgactttca
    10741 ccacaagcag taagaagaaa tccagtagct agcagacagg tagtaacaat aatttttttc
    10801 atttttttcc acctcttatt ttattatttt cagccacata tattcatacg gctggaatat
    10861 caactttcgt tcttgactgc aatggataat ttgtccagaa aacaggtcta ggtaatctcc
    10921 ttttggtaaa agataacctg ctgattgatt ggaaaaatta tggataacta agactttctc
    10981 ttccttcgct tcgcgaataa aactgataaa agaatctgct gagtccagta agtaaatcaa
    11041 actattcgga tggaaggctt tttctttttt tcgaatagat agcatctggg tcaattcacc
    11101 atatactgct gttcttaatg gatctgtctg caactctttc tcaatatctg ctaacaaaaa
    11161 tttttgccga ttgatttttc gattctctcc tgttttttct acgcctttgt aatcatttcg
    11221 acttcccagc acactttgaa tataaattgc cggcacgcct ggtaacgtta ccaaaaatga
    11281 atgagcagct aaaaagcgtt tgcttctcca ttccgtcgta tctgttggag aacttaacgc
    11341 atctagatac gtagcgttta tctcatatgg actggtcgtt ccatctgggt ttttcttgta
    11401 gtttactcgg gcaccctctt tcttcagctg ttccactaat tcaaggatcg ttttttccgg
    11461 cagaatacct cgtaccggat tcaatccgat accgtcatgg gaggctaaaa aattaaaaaa
    11521 agttgttttt ttcgatgcga aatcgatggt ttttaaccat tctttcaact tacttgtatt
    11581 acccgaacga atcgcttgaa gggtcaaagg tggtagcgaa aactgataga ccatctgtgc
    11641 ttcattctcg ccattcccaa aataactgat attatctttg tgtggcacat ttgtctcagt
    11701 gatcaatacc gttcctggag ctgcttcatc agcgattgca cggaataatt ggatgatttt
    11761 atgcgtttct ggcaggtgta tacaagaagt tcctagttct ttccataaaa atcccacagc
    11821 gtccaagcga ataaatgaag cgcctttttc taagtaaaac aaaagaatat cgatcatttt
    11881 caataaaact tctggatttt taaaattcaa gtctacttga tcttcgctaa aagtcgtcca
    11941 tacgtgtttt tctttcccgt ttttgtcaat gaacgttgta agtaacgggc tggtccttgg
    12001 tctagtcacg ctgcttgtat ctgtatccag tggcaaatca ataaaaaaat ctttgtacgt
    12061 atcattcaag tttaaatatt cctgaaacca agtactttcg gcagacatat gattgcagac
    12121 aaagtcgaac attaaccgtg tttcgcttct catttcttct atatcttccc atgtgcccaa
    12181 acggtcgttg attgcctgat aatttacaac ggagaaacca tcatcagatg tatatgggaa
    12241 aaatggcaag aaatgtacaa ctctaaatgt atctgccaaa tacttttgat aaaatttctt
    12301 aaatgtacgc aaagttggtt gctgctcttc aaaaaattga tccccataag taatcaagaa
    12361 aatatcttct tcgtcccaat gcagtttgcc tgcctggcct ttcttctgct gataaacact
    12421 gactttttgt tcaatcatag aggcggtttt ctcagacaaa tctgagtcgc gataaatcgc
    12481 ttgtaaaagt tttgtataca tcggattccc ctttcttttt tgggagcgtt cccaacgatt
    12541 tgatcatatt atggaaaacg cttactgtca atagcatttt tttaaataat tttgatatac
    12601 acaaaaatac aaataagaaa gcttatgtat caataattaa tagcttctta aaaaaatatg
    12661 gtctctcatg atcatgcaag ctattcctaa attttcaaaa tcatagacaa aaaagaatgt
    12721 ctattgtact atggaattat tctaaaacgc tcccaaaaag gaggtgcata taaaattgaa
    12781 acctactatt tatgacattg cgaaaaaagc taatgtctca aaatcaactg tctccagagt
    12841 attaaataac cagccaaata tctctgataa agcaaaagca agtgtacttc aagcaatcaa
    12901 agaattgaat tatgaaccga gcaaaattgc aagaggactg tcaggaaatg gttttgacgc
    12961 gattctagca ttatctcatc gtacttctca tacgacctat ggcaatcctt ttttttcaga
    13021 tgttattcaa tccatttctg ccgtttctga acaaaataac ttcgacttga ttctacaaac
    13081 ggcaaaaaca ccagaagaag agctagaaaa atgcgcgaaa aagatccaag aaaaaatgat
    13141 ccgaggaatt attattttaa gctctacgat ggatgaaaca cttctttttg aattggatca
    13201 atacaatatt cctattgtgg taatcggaaa agtcggaaac gactacacga acatctactc
    13261 agtggacacg gataatttcc aggatagtta cgatttggtg gaaacactga tcaagaatgg
    13321 acaccgtcat atcggctgtc tccatgctcc gacagaagtt tatgtatcca atgatcgtct
    13381 caatggttac cgtcaatgct tatttgatca ccacctagat atccgtaatg aatggattat
    13441 agacggcggc tttcgcttag aagatagcat ggcagcaatg aatgatttaa tgaagctacg
    13501 agaaagacca actgcgattt tttctaccga tgcattaaaa tcattgagta tttacaaagt
    13561 ggcagaacaa tctggaaagc agatacctga tgatctttcg atcgcaggat tcagtgatcc
    13621 cttattgtcc tccctatttt cgccacagtt gactcggata gacattccga caaatcagtt
    13681 aggaatcaaa gccacagaac ttttattcca actaatccac caccaatacc ctgaagaaaa
    13741 aagaatcatt ctttcaaccg agattactct cacagattcg atccaacaac aagaaatgta
    13801 gaacagaatt ataaaagaac agaaacactg aggataagtc agttactctg ttcttttttc
    13861 aatcactcat ccctcgttac cccctcccgg tattttagcg aatttttctt attttccatc
    13921 tgttttagat agctgtggat aactataatg tttgtgttaa actatttttt ccacagggaa
    13981 gaaaaaacta tttttaacca cgttgtgtat aagtttttat agttcgtttt tttaaacaca
    14041 tttaccctta tttcaatcca tgttatttgg ggatacttat ctctaaaaca taaatagaat
    14101 aacagacatg tctactggtt ttgttttatt cgaggaatta ttcaataata gctagcaaaa
    14161 tattaaaaat aaccgctcta tcttcccatc aatttatttt gcattcaaaa aatgatctat
    14221 ctttactttt gttgattgac ttctcttgga agatgatcgt tccgatacct taattggata
    14281 cgaaccgatg ttgttaagat cctttcccac ttttcttcta gttagacaaa ttattttaga
    14341 caaaccaaaa aagaggttgt gacaataatt tgtcacaacc tcttttttaa aatatctatt
    14401 ttgtttgatt gttcattgcg tcttctgcag ccatttgcgc ttcaatatct gaaatactgt
    14461 agacattttc gcttgctaca ccgacttctt ttggttccat gtcacgataa cgttgcatac
    14521 cagtaccagc tgggatgatc ttacctataa taacattttc tttcaaacca agaagaggat
    14581 ctttcttacc gcggatcgca gcatccgtca agacacgagt tgtttcttgg aatgatgcag
    14641 cagataagaa actatttgtt tctaatgatg cttttgtgat acctagtaag acaggtcgag
    14701 aagttgcagg aacgcctcct gagactagcg tattgtagtt ttgatcttta aagtctgcga
    14761 tgtctaataa tgtacctggt aagatatctg tatcacctgg atccattaca cgaactttac
    14821 gcagcatttg acgaaccatt acttcgatat gcttgtcgcc gatttctacc ccttgcatac
    14881 ggtaaacacg ttgtacttca cgaagcaagt agttttcaac agataataca tcgcgaactt
    14941 gcaacaattg ttttggatcg attgatcctt ctgtcaatgg tgcaccacgg tggatcatat
    15001 cgccttctgc tactttcata cgtgctgtat aaggaacagt atacgtacgt gtatctgttt
    15061 ttcctttgat tgttacttct ttggtacgtg ttgcaggatc ttctgagata tcgatgacat
    15121 cccctgttac ttctgtgatc actgcttgcc ctttcggatt acgtgcttca aagatttctt
    15181 ggacacgagg aagaccttga gtgatatcgt ctccggcaac cccacctgta tggaatgtac
    15241 gcatcgtcaa ctgagtacca ggttcaccga ttgattgagc ggcgatcgta ccaactgctt
    15301 ctccgacttc aacgtcagag ccagttgcaa ggttgcgtcc gtaacaatgt ttgcagacac
    15361 catgttttgt attacatgtg aagacagaac ggattgtcac tgtttcgaca cctgcatcaa
    15421 cgatttgttt tgcgatgtct tcggaaatca attggtttcc tgcaatgatc aattcatttg
    15481 tttcaggatg acgtacttct ttacgggtgt aacgtcccac taaacgatct tctaatgatt
    15541 cgatgatttc atttccctcg cggatggctt ggatttcaag acctcggtca gtaccgcaat
    15601 cttcttcacg gatgattaca tcttgggcca cgtcaaccag acgacgagtc aagtaacctg
    15661 aatcggcagt cttcaaggct gtatcggtca tccctttacg agccccgtga gttgagataa
    15721 acatttccaa gacggatagt ccttcacgga agttcgaaat gattggcaat tccatgattc
    15781 gaccattagg agcggccatc aaaccacgca ttccggcaag ctgtgtaaag ttggaaatgt
    15841 taccacgagc tccagaatca gacatcatga agattggatt tttcgcttcc aaaccttcca
    15901 tcagtttttg ttggatgctg tcttttgcag cattccaaac agcaatgaca cgttcataac
    15961 gttcgtcatc tgtgatcaga ccacgacgga attgttttgt aatagtttca acttgtttat
    16021 gggcattttc aatgatttct tgtttttcat tcagtacact gatgtcagcg atccctactg
    16081 tgataccggc atgtgtcgaa tgtttgtacc ctaagtcttt catacggtca agcatcttag
    16141 aagtttctgt tactttgaat cgtttgaaga cttctgcgat gatgtttcct aagtttttct
    16201 tcttgaacgg taatactaat tcttgctctt tgatatgtgc aggaatatca gtaccagctt
    16261 caacaaagta tttgtccggt gtttggacgg tcaagttgaa gtctgttggt tcattcagat
    16321 aagggaattc aggaggcatg atctcgttga agatgatctt accaactgtt gtgatcatcg
    16381 tacgttcttt ttgccattca gtaaatggtt tttcacctaa tgagttaggg ttcacaccga
    16441 tacgtgaatg taaatgaaca tatccgttgc gccatgcgat aacggcttcg ttttgatctc
    16501 tgaagaccat tccttcgcct tcgcgtcctt cttcttccat tgtcaagtag tagttaccta
    16561 gaaccatatc ttgagaagga gtaacaactg atttaccatc tttagggttc aagatgtttt
    16621 gagcagctaa catcaacata cgtgcttctg cttgtgcttc ttcattcaat ggaacgtgga
    16681 cagccatttg gtctccatcg aaatcggcat tataagcttc acatacaagt ggatgcaaac
    16741 ggattgcgcg tccttgtact aaaactggtt cgaatgcttg gataccaagt cggtgaagcg
    16801 taggtgcccg gttcagcaat actgggtgtt ccttgattac gtcttctaaa acatcccata
    16861 cttcgtcttc ttgacgttcg attttacgtt tcgcattttt gatgtttgaa gctagttcgc
    16921 gttgaacaag ctcacgcatc acgaatggtt tgaataattc aatggccatt tcttttggaa
    16981 gtccacattg gtacatcttc aagaaaggtc caacaacgat aacggaacga cctgagtagt
    17041 cgacacgttt acctagcaag ttttgacgga aacgaccttg tttccctttc aacatatgag
    17101 aaagagattt caatgggcgg ttacctggtc cagtaactgg acgtccgcga cgaccgttat
    17161 cgatcaatgc atctactgct tcttgcagca tacgtttttc attttgaacg atgattccag
    17221 gtgcattcaa gtctaacaaa cgtttcaaac ggttattacg gttgattaca cgacggtaaa
    17281 gatcattcaa gtcacttgtt gcaaaacgtc caccttctaa ttgaaccatt gggcgtaaat
    17341 ctggtggaat aacagggata acgtccataa ccatccagct tggttgattt cctgaagcgc
    17401 ggaatgcttc taaaatatcc aaacggcgga ttgcacgtgt acgtttttgt ccttgtgcag
    17461 atttcaattc ttctttcaat tcggctgctt ctgcatctag atcaacatta tcaagtaatt
    17521 gtttaatcgc ttctgcaccc atagcagctt ggaattcttg tccatattgt tcacgttttt
    17581 cgcgatattc gcgttctgtc aacaattgtt ttttctctaa attcgtattt cctggttcga
    17641 tgactacata tgaagcaaaa taaatgattt cttccaaagc gcgtgggctc atgtctaata
    17701 caagacccat acgtgaagga atccctttaa agtaccagat atgtgaaaca ggagctgcta
    17761 attcaatatg tcccatgcgt tcacggcgaa ctttcgaacg agtaacttcc actccgcatc
    17821 gatcacaaac gattccttta taacggatac gtttgtattt tccgcaggca cattcccagt
    17881 cttttgtagg accaaaaatc cgctcacaga acaacccttc gcgttcaggt tttaaggtac
    17941 gatagttaat cgtctcaggt ttttttactt caccgtaaga ccagcttctg attttttcgg
    18001 gagaagccaa acctatttgc atactttcga atttatttac atcgatcaaa aggggttccc
    18061 tccatttcat ttaatggcaa accgcaagac ggctgatctt cgaccgcctt gcagctcata
    18121 gaccggatta gtctaaatta tcttctgcgg tttcaaagtt ttggatgaca tcgtcttttt
    18181 catcttccac ttgttcagca gctttctttt ctaattcttt ggcagtttgc tgttcggcaa
    18241 attttgtcaa agcatcaaca gtgatcaaat cgtcgtcttc atcgtccatg tcgcgaagtt
    18301 cgatctctgt ttcttcgata tccaagacac gcatgtccag acctagtgat tgcaattctt
    18361 tcaccaatac gcggaatgat tccggtacac ctggttttgg aattggttca cccttgacga
    18421 ttgcttcgta tgttttcaca cgtccgacta cgtcatctga tttgtatgtc aagatttctt
    18481 gtaaagtata agccgcaccg taagcttcca gtgcccatac ttccatttca ccaaaacgtt
    18541 gtccaccaaa ttgagcttta cctcccaatg gttgttgtgt aaccaatgag taaggtccga
    18601 ttgaacgtgc atgcaatttg tcatcgacca tgtgagcaag tttgatcatg tacatcacac
    18661 cgacagacac acgtccatca aatggttcac ctgtacgtcc gtcgtacaga acagttttcg
    18721 catcgctggc cataccggct tcacgaactg tttcccatac gtcttcatca cttgcaccat
    18781 caaatactgg cgttgctacg tggataccta actgacgtgc agccataccc aagtgtaatt
    18841 ccaatacttg cccgatgttc atacgtgaag gcacccctaa aggattcagc atgatatcga
    18901 ttggtgttcc atcaggtagg aatggcatat cttcttccgg cataatacgg gatacaaccc
    18961 ctttatttcc gtgacgaccg gccattttat ccccttcatg gattttacgt ttttgaacga
    19021 tataaacacg aactaacatg tttacgcctg gtgacaattc atctccagct tcacgagtaa
    19081 agattttcac atcgtgaacg ataccgccgc cgccatgtgg tacacgtaat gatgtatcgc
    19141 ggacttcacg agctttttcg ccaaagatcg catgcaatag acgttcttct gcagataatt
    19201 ctgttacccc ttttggcgtg actttcccta caagcaaatc gccatcttgg acttctgcac
    19261 caatacggat gatacccatt tcgtctaaat cttttaatgc gtcttccccg acattaggga
    19321 tttcgcgtgt gatttcttca ggtcctaatt ttgtatcgcg ggcttctgat tcatattctt
    19381 caatatggat agaagtgtat acgtcatctt ttaccaaacg acggctcatg atgatcgcat
    19441 cttcatagtt gtacccttcc caagtcatga aaccaactaa aacgttttgg cctagagcca
    19501 tttcgccttg ttccatagat ggtccgtctg ccagagtatc ccctttttcg actttctcac
    19561 ccaaatgaac gattggacgt tggttgtagc ttgttccgga gtttgaacga cggaattttg
    19621 ttacatcgta tttatctaat gcaccgttgt cgcggcgtac gcggatttca gaagcatcaa
    19681 cgtattcaac gacaccgtca tgtttacata acaaagcagc tcctgagtca tgcgctgatt
    19741 tgtattccat accagtacca acccatggtg cttgaggatt gatcaacggc actgcttgac
    19801 gctgcatgtt ggcacccatc aaggcacggt tggagtcatc gttttccaag aaaggaatac
    19861 atgctgtcgc aacggcaact acttgtttag gtgatacgtc catgtagtct actttatcaa
    19921 tagatacttc taagttttca ctttgagcac gagccataac aacgtcttga gcaaatgttc
    19981 catcttcatt caatgggctg tttgcttggg ctacgatgta atggtcttcg atatcagctg
    20041 tcaagtagtc gatttggtca gtaacacgac ctgtttcacg gtctacacga cgataaggtg
    20101 tttcgataaa tccgaattta tttactttcg cataactgga caaactattg atcaacccga
    20161 tgtttgggcc ttcaggagtt tcgatcggac acatacggcc atagtgggag tagtgaacgt
    20221 cacgaacttc ataaccggcg cggtcacgag tcaatccgcc aggtcctaag gctgaaagac
    20281 gacgtttgtg tgtcaattcg ccaagagggt ttgtttgatc catgaactgt gacaactgag
    20341 aagaaccaaa gaattctttg atacttgcca caactggacg gatattgatc aattgttgag
    20401 gtgtcaatgt ttcagtatct tggatcgaca ttctttcacg aaccacacgt tccatacgtg
    20461 ctagcccgat acggaattgg ttttgtaaaa gttcaccgac tgaacggata cgacgatttc
    20521 ctaagtggtc gatatcatcg acattgccga taccttccat caagttcaag aaatagctca
    20581 ttgaagcaat gatgtcggct ggacgaaccg tttttacagc tgcttctgga tagccgtttc
    20641 cgatcacgtt tacttcacgt tcagggtctc ttggtgaaaa tactttgatg acttggacag
    20701 tcattggatc tgtgaccaca ccgtcttctg atggatagta agtcactgaa tttaggccat
    20761 tatcaatgaa aggagctagt gtttccatga cttggtgagt cagaactgtt cctttttcaa
    20821 cgatgatctc gcctgtttct ggatcaacta atgtttcagc taatgtcaga ttcaatagac
    20881 gtgttttcaa atctaatttt ttgtttactt tgtaacgacc aacgtttgct aagtcgtaac
    20941 gttttggatc gaagaaacga gcattcagca agttacgtga gctgtctgct gtttttggtt
    21001 cgcctggacg caaacgttcg taaacgtctt tcaaaccttc ttctgttctt gagtcgcttg
    21061 cgtttttatg caagtctttt tcgactgtat tgcgtaatgt ttcgctgtcg ccgaagattt
    21121 caaagatcgt atcatcggaa ccaaatccta acgcacgaac taatacggtt aaaggaatct
    21181 tacgtgtacg gtcgatacgt acataagaga tgtcttttgc atcagtttcc atttctagcc
    21241 aagctccacg gttagggata acagtagaac cgaatccttc ttttccgttt ttgtcgactt
    21301 ttccatggaa atagacacct ggtgaacgga ctaactgaga aacgattact cgctctgccc
    21361 cgttgatgat gaatgttcct tgttctgtca ttaatgggaa atcaccgaag aatacttctt
    21421 gtgctttgat ttcgccagtc tcgcggtttg tcaaacgcaa tgttacatgt aatggtgcag
    21481 agtagtttgc atcatgcgca cgagcttctg ctactgtata ctttggttct ttcaattcat
    21541 aatctacaaa ttccaaagat agattgccat tgaagtcatc gattggcaaa atgtcttcaa
    21601 acatttctct aagtccctca tctaagaacc attggtaaga gtctgtttga atttcgatca
    21661 aatttggtaa ttccaacact tcactgattc ttgcgaaact tctacgttcg cgatgttttc
    21721 cgtattttac tacgtgtcca gccaagctct tcacccctct aaaatgtcat tcaaaaagac
    21781 tacccgatca attgttacaa taatatttca aatattattt ttaggtaaca acagacattt
    21841 tttgcggctt ttaggcaaaa aaaaaccaaa aatagaacat tctcttgaaa ccttacttcg
    21901 tgagcttcta cttttgtttc cttatattaa agccgaaacg gacaatattt cgtttctgcc
    21961 ttttcttcat cagatagttt ttgcaacttc atatactaca cttattttct ataaaagtca
    22021 acacttcctg gcgtttcttc tgctttttcg caacattttc ttaatgtttt caattttatg
    22081 ttttcaatta tctgacaatt cttaattgac agaccttttt atctcggtta tactttagaa
    22141 aattcatttt gtggaggaac ttctatgcga accaacatca accaaatcgc accaacacaa
    22201 ctgaataact tttactttag ctactttaga taggtttgta ttcatcatga tggatacaaa
    22261 cgttaaacga cagtttgtat ctatgatggc taaagaactt tgtattgcag caaaaagcca
    22321 cgtagacaaa atgctaagtg gctgagcagt gaaaatacaa agtaaaagta tcaagcattc
    22381 tactttttca atttattttc cgacttttca atccatttgg acattttgtc tgaatggatt
    22441 ttttgtttcc caaaattaag gaggaaaaag aaatgaaaaa aaatataatc atcggattag
    22501 gcgctgccct gctactagct ttaggaggtt gcgcacaaaa agacaattcc gcagaggcat
    22561 cagatggaca aaaaacaatt ggcgtattgc aacttgtaca acataactcg ttagatgctg
    22621 cctataaagg attcaaagaa ggattagcag aagaagggta taaagaaggc gataatctaa
    22681 caatcgatta tcaaaatgcc caaaacaatc aagacaacct gaaaagtatg agtgagaaac
    22741 tcgtcaaaga agatcctgat ttgctacttg gaatcgccac tccagcagca caatctttat
    22801 taaatgaaac aaccactatc ccgatgactg tcacagcggt cacagacttg gaacaagccc
    22861 atttagtcac atccaatgaa aaacctggcg gcaatgtgac tggcacgaca gatatggtgc
    22921 caatcgaaaa acaaatcggc ttactgttaa atatcgtgcc agacgccaaa acaatcggca
    22981 tcatgtataa caacggtgaa gcaaactcta agatccaagc tgatttggca gaaaaagaat
    23041 tgaaaaaagc gggcgtcaaa gtcaaagtac tgacagcaaa ttccacaaat gatgtccaac
    23101 aagtgacgaa aagtctagct aaagatgtag atggaatcta tattcctact gataacacgt
    23161 tcgcaagtgc ggcaagtgtt gtcggtgaag tcgcaaaaga aacaaaaaca cctgtcgttg
    23221 ctggcgcagt tgatcaagtc aaagacggcg gtctagcaac agtaggaatc gactacgaaa
    23281 gcttgggcaa acaaacaggg atcatggcag caaaaatctt agacggcaag aatccaagtg
    23341 aacttcctgt cgaaacagcg aatgatttgg aactgtatgt caataaagac atggccaaag
    23401 cattaggcat caatccagac agcatcaaag aagctgaata aaacacagga ggattctcat
    23461 gacaaatttg ctctctcttt tcttatcatc tgcatcgcaa ggggtcttat gggccctact
    23521 tgcgattggt gtatacgtca ctttccgtat cttggacatt gccgacttga ctgctgaagg
    23581 aagctttcct ttaggtgcag ggatcgcggc agtaagtatt acaaatggct atcatccatt
    23641 gacagcttgt cttctaggct ttttaggggg tgcggcagca gggcttgttt ccggactgct
    23701 tcacacaaaa ttgaaaattc ccgcccttct tgcagggatc atcaccatga ctggtttata
    23761 ttctgtcaca tcccgtgtga tgggtgcgcc gaacatctct ttattgggtc agccaacgat
    23821 ctttcattgg gtcgagtcat tcggcataac aaaagacaac gctgtactga tcgtcggtct
    23881 gctttttgct ttgatcgttg tatgtttact tgttttgttc ttacgtacag aaacaggact
    23941 tgctattcgt gcaactggcg ataatctggc aatgagcgaa gcaaacggga tcaacacaga
    24001 taatatgaag atcatcggtt atatgatttc taatggatgc attgctcttt ctggctcctt
    24061 gcttgcgcaa aacaacggct ttgctgattt aaattctggt attggaacga tcgtaattgg
    24121 acttgcttct attatcatcg cagaagtttt gttacgtaac cagccactat ttctccgtct
    24181 cttgactgtc gttttaggtg cggtcattta tcgttttatt ttagcgttag tcttcgaact
    24241 gaatgttgaa ccaagtgatt cgaaacttgc ttctgctttc gtgctagtga tctgtctttc
    24301 tttcccgcag atccagcaaa aattccattt acctcaaaaa aagaaaggcg gtagtgtcaa
    24361 atgaatgacg tcttaacatt ggaaaatctc catcatacct ttgaaaaagg aacgatcaac
    24421 gaaaatcatg tcctgaaagg cattaatctt tccttagatc ccggcgactt cgtgacgatc
    24481 atcggtggaa atggggctgg taaatctact ttgctgaaca gcatcgccgg tacattttct
    24541 gtcgatcagg gtcaaatttt attgaatgga aaaaatatca cgaaaaaatc tgttgtcgaa
    24601 cgttcgaaat ccatcagtcg cgtattccaa gatcctaaac tagggacagc cgttcgttta
    24661 acggtagaag aaaatcttgc tcttgctatg aaacgaggaa aaaaacgcgg cttcttccgt
    24721 ggtgtcaaac cacaagaccg aacatttttc aaagaccagc tggttcggct aaatttgggg
    24781 ttggaaaatc gtttatcctc agaaatcggt ttgctgtctg gaggacaacg tcaagctatt
    24841 acgttattga tggccacatt acagcgtcca gaactgatct tattagatga acacactgca
    24901 gcgcttgacc ctgctacttc caaaacggtg atggaattga cagaaaagct gatcactgag
    24961 cagaatctca ctgcttttat ggtcactcat gacatggaag acgcgatccg ctatgggagt
    25021 cgactgatca tgctgcatca aggaaggatc gtcgtagata tccagcaaga ggaaaaacag
    25081 cagctcacag tgccccaatt attagcgttg ttcaaacaaa acagcggcac ggcattaaaa
    25141 gacgatcaag tacttttatc ttcataatca gaaagaggat gggac
//
LOCUS       DAFEPQ010000037        24355 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.037, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000037
VERSION     DAFEPQ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.037"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(132..2477)
                     /locus_tag="LP543_001618"
     CDS             complement(132..2477)
                     /locus_tag="LP543_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBL2321787.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2756..3658
                     /locus_tag="LP543_001619"
     CDS             2756..3658
                     /locus_tag="LP543_001619"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBL2321788.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3869..5644
                     /locus_tag="LP543_001620"
     CDS             3869..5644
                     /locus_tag="LP543_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBL2321789.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5710..7296)
                     /locus_tag="LP543_001621"
     CDS             complement(5710..7296)
                     /locus_tag="LP543_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBL2321790.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7547..7771
                     /locus_tag="LP543_001622"
     CDS             7547..7771
                     /locus_tag="LP543_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBL2321791.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7913..9664
                     /locus_tag="LP543_001623"
     CDS             7913..9664
                     /locus_tag="LP543_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2321792.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9664..11448
                     /locus_tag="LP543_001624"
     CDS             9664..11448
                     /locus_tag="LP543_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2321793.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11783..12635
                     /locus_tag="LP543_001625"
                     /pseudo
     CDS             11783..12635
                     /locus_tag="LP543_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            12732..14618
                     /locus_tag="LP543_001626"
     CDS             12732..14618
                     /locus_tag="LP543_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL2321794.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14659..15561)
                     /locus_tag="LP543_001627"
     CDS             complement(14659..15561)
                     /locus_tag="LP543_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL2321795.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            15791..17194
                     /locus_tag="LP543_001628"
     CDS             15791..17194
                     /locus_tag="LP543_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL2321796.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17191..18627
                     /locus_tag="LP543_001629"
     CDS             17191..18627
                     /locus_tag="LP543_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL2321797.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18772..19698
                     /gene="trxB"
                     /locus_tag="LP543_001630"
     CDS             18772..19698
                     /gene="trxB"
                     /locus_tag="LP543_001630"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBL2321798.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19911..21638
                     /locus_tag="LP543_001631"
     CDS             19911..21638
                     /locus_tag="LP543_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBL2321799.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            21654..22004
                     /locus_tag="LP543_001632"
     CDS             21654..22004
                     /locus_tag="LP543_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBL2321800.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22098..22958)
                     /locus_tag="LP543_001633"
     CDS             complement(22098..22958)
                     /locus_tag="LP543_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBL2321801.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23050..23478
                     /locus_tag="LP543_001634"
     CDS             23050..23478
                     /locus_tag="LP543_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL2321802.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23640..23816
                     /locus_tag="LP543_001635"
     CDS             23640..23816
                     /locus_tag="LP543_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBL2321803.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23843..24289
                     /locus_tag="LP543_001636"
     CDS             23843..24289
                     /locus_tag="LP543_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBL2321804.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7713 a         4357 c         5190 g         7095 t
ORIGIN      
        1 gtttttcaca tttttacata aatttgtctt aatatcgtag atgtaagctt ttgaacacca
       61 tttattcgga aaaagggagc tgtgacaaga tttttgtcgc agctcccttt aattctgatt
      121 aatctattag atcaatcatc tttatcgtct gattctttgg tctctggatt cgctctagca
      181 taagctgaag cggtgttcca gtaatctgta tagttttcat cagtaccgcc tacgccgaat
      241 ttaaaggcgc ttttcttcgg accatttttt gcccaaagtg actccactgt ttttcctggc
      301 gtttggatgg attttttatt gacagtaact tttccagctg tttgtcctgt gaacccagat
      361 acttttttct tcatcacatc ggatgataag tcaaatttct catcagtgcc gaaaatctca
      421 gggtttgctt ggtaagcttg attgattaga ttggcaagat aattagccga acgtattctt
      481 gcttggctgt ccattccagt attgtcatcg tgtcctgccc aactactgat agtgatagat
      541 ggagttgaga cgatcagcca tgaatctcgg tattcattgg tagaaccagt ttttccgacc
      601 caatctgcat ttcctagatt tccgtttaag cttgagatga catctttgaa tggtgtagtg
      661 attttttcat tgatcacact acgcatcatg tcattcatga tcgatgccgt tgcttcggaa
      721 taaatacgga cagaattact ttcatgttta taaatcacat tacctgcatt atctgtgatg
      781 gaatcaatga tatagccttc ttcgtagact ccaccatttg ctaaggcctg gaatccattt
      841 gtctgttgaa gcgtcgttac atctactgtc cctaaaggag ccgattcaac gccccaatta
      901 tcatttgctg ggtagttcat cttcgccaag tagtgctcgt aagcgtattg tttcgatcca
      961 ccggaattca acacatcttg atataagtga taagctggga tattgttgga ccattccagt
     1021 gactcacgga cggtttggaa ggtgttcgac ccttcattgg tcgcattgac gattttctca
     1081 ccagcatttt ttccttcttg ccaagtcgtt gcataatctg atacacggga ttctgatccg
     1141 acaagtccca tatcgattgc aggaccgtaa acaagaaccg gtttgatgga tgaaccagct
     1201 tgacgttctg tatcaaaggc gtggttattt tgattctggc tgtaattccg accacccacg
     1261 aagccataga tacgtccggt tctgttatcc atcaatacat ttccagtctc cacttggcta
     1321 ccagcaccat catctaacat gtagccataa ttttctacgc cggtttgtaa tgcagcatag
     1381 atatctttat cgatcgtcga atggatcgta tatcctttgt tttggatcgt ttgctgtgct
     1441 ttttgataat aagcttcact gaccgatgaa tctgacatat cagcattatc tttttctgcc
     1501 aattgttgtg caataatcct tgttgcttcg ttcatcacgg tataatacaa gaattctctg
     1561 tcagtctgtt ccgcgatttg ttgtgggaga aagtcttttg taagatcata ggctttcgct
     1621 tcttcgtatt gttctttcgt aatttgtcct tcacggtaca tactgaataa aacaaaatct
     1681 tttctggcaa gaccggcaga aagatcttct tttagcgcgc ctgtattcgt gtatggacta
     1741 tacgtgatag ggctttgagg caaaccagcg atataagcag cttgtggcag agtcacatcc
     1801 tttgcatcta caccaaagat tcctctagca gcttcctgga ctccagcaat attttgcccc
     1861 ttattattcc gaccaaatgg tgagacattc aaatacgtag taatgatctc attttttgaa
     1921 aagtgttttt ctacttgtgc agaaagaacg atctcgtttg ctttacgttt gaatgttgtt
     1981 tcatcggtta ggatctgttg tttgactaat tgctgtgtca aagtggatcc tccagacgag
     2041 ccgatgccag tcgcttctga aactagtgct cgtaaaacag ctttagggac ataaccttga
     2101 tgtttatcga agtattcatc ttctgtactg ataatcgcgg ttttcaataa aggcgaaatc
     2161 ttatcagatg aaatagtcgt ccgcatcaga tctgtctgga tttttgatat ctctgtatta
     2221 tctgcataaa caatctttga cgtttccgta atgtttccta gttctgtctg cagttctttt
     2281 ttagtcggca attgtgtgtc ttcgacaaga taggcaaagt agccagcacc tattccgata
     2341 cctagagctg cgaacaacaa aagaagaaca gtaataaaaa cagtcaatga ttgaaacact
     2401 ctaaaaataa tcttaggaac aaaccatttt tctttttttc ttctattctg tttttttcga
     2461 gtatttttgc gtggcaaaag ggtcacctca actaatggtt ttatttcgct aattatagca
     2521 aataatagga caaaaaaaca gacaaggctg tttttgacag gaatttcttt ttcgaatgag
     2581 ataaaacgtg aggaaatgat cttttttatg aaagagttct taagcttttc tttagggaaa
     2641 gtttcagtta ctatggagaa cagggcaaag aaaaacaaac cattttcttt gtacatcatc
     2701 ttatcaatat acaggaattg ttcgaaacaa ttgcctttca aggaggaatc catccatgtt
     2761 attaggctca catgtcagca tgagcggaaa gaaaatgctg ttaggcgcgg cagaagaagc
     2821 agccagttac ggatcttcga cctttatgat ctatacaggc gctcctcaaa atactagaag
     2881 aaaagctatc gaagagatga atattgatgc cggaaaagca tttatggcag aaaacggact
     2941 aacaaatatc gtcgttcatg cgccttatat cattaatttg ggaaatacga tcaaaccaga
     3001 aatgtttcca tttgctgtac aatttctgag agaagagatt gtccgtgcgg aagctttagg
     3061 tgctactcaa atcacccttc acccaggcgc tcatgtagga gcaggggcag aagctgggat
     3121 tgctagaatt attgaggggt tgaatgaagt tttgacaaaa gaacagacgg cacagatcgc
     3181 tttggagacg atggcaggga aaggtacgga aatcggtcga acatttgagg aattagcgca
     3241 aatcatcgat ggagtaaaat tgaatgaaaa attatccgtt acttttgata catgtcatac
     3301 gaatgatgcc ggatataata taaaagaaga tttttcaggt gtaatggaag agtttgataa
     3361 ggtcatcgga ttagatcggc ttaaagtact gcatatcaat gattctaaaa atccacaagg
     3421 ttctcataaa gatcggcatg ccaatatcgg ttttggaaca attggatttg acacattgaa
     3481 caaaatcgtc catgatccaa tgtttgacaa catttcgaaa atattagaaa ccccatatgt
     3541 cggtaaagat aaaaagaaag cttacgcacc atatggaaaa gaaatttcca tgttcaaagc
     3601 gcaagcattc gatcccgaag tgttccctga gttattagca gaagaagtca cactttaagt
     3661 tttctacctg acagcaacaa aaaaggattg ggcagtctgc cttgtccttt tttgtttaag
     3721 aaacaaagga gtgctttttg cattcgtttt cgccatgctc cttttgcccc ctctttttaa
     3781 attcagacaa acagggtata ctgatagaaa agtaaaaaac gaaaagtttc acaaacaagt
     3841 agcaaaacgt gattgaaagg acgaaaagat gctgaacatt ttaaacccct tgatcgacca
     3901 actaaaaaag aaacatacat actttattct tggcatagcc gtattattta caattattcg
     3961 gttgtattta tatagtaaag caatctatgg aacagatatg tcggcgacct atgatgatca
     4021 gctgctgatt cactatgctc aaaatatttt ggatggtaaa tggctagggg agtatacgac
     4081 tacaacactt tccaaaggaa tctcctacag cttattttta gtacttgcac ataaactgca
     4141 tctttcttat gcagttttgt tcggcatgtt cagtattttt gccagtatat tggttgcact
     4201 cgcgtttaaa ccgattgtga agaacaagta tcttctttta agtatctatt tttatttttt
     4261 gttttctccg attagtttta cacatgaata ttccactcga acatatcgta atgccctcgt
     4321 gattccggct gttgagatca ttatcgcttg tttattagct atttattacc gcaaatacgg
     4381 ttctagcaga aaattgctgc catggttttt agggctttct gtcgcttttc ctttcttttg
     4441 gttcattcgc gaagattcta tctggcttct tccttttact gtagtagctt tgggtatcag
     4501 tttcgtccag atcttgctag ggcaagactt ttctttcaaa gaagtaaaga agttaaaaaa
     4561 acgactcttt cttctctcga aacgggaatg gctgaaactt tcattgtttt tacttcctat
     4621 agttggcttt tccatcgtct atcagtggat cgaatataaa aatgagacaa catacggtat
     4681 tgcaaccatc aatgaccgtt ccagtggaga atttggacgt ttgatgaaac aactgatccg
     4741 aatagatgat gaaacgaact tgaataaaga aaacagtgag atatgggtct cacatgctgc
     4801 actaaataaa gcattagccg catctcctac attccatact gtttctaccc gtattgacga
     4861 attatatacg actcacgcgt ggactcaagg tggaaaagta aaagaattac ccggagatat
     4921 catcttttgg gcattgcgag atgtggtaag cgaatctggt tattaccaaa acaatgcaaa
     4981 agaaaccaat gatttttgga aaaaagtcaa ccaagaatta gaagaagcct atcacaaggg
     5041 aaccatcaaa aagaaaaaag agttctatct gatgggatca ggagatggaa agacgatgag
     5101 tgatctgcca atcgtattcg atttccttca atcaggatac gcttatgcat ggcgatataa
     5161 agatttccat caaggaggaa attatagttt cggaacgcaa gaacaagtca aacaggctca
     5221 agaattatta aaccttccgc taatggagaa ttggatcgat agtaatgaac cgagaacacc
     5281 taatttccaa ttaacaaaat cagcaaaagt cgcgaatgtc atcataaaaa tttaccaagt
     5341 tctggctatt cccgtgctga ttgcttcaat agcagggata ttacttatga tactaggaac
     5401 cgtatttgca aaagctgatc gtgcctatta tagttcctct ttgattgtgg tcaccggatt
     5461 aatactgaca caattggttt ttcttttcgg tgtctcatgg ttttgttctt atgcgccaga
     5521 aaaaaaggac tattttttaa gtgtctacac tggtgcaggc gtgccattaa cgcaattcat
     5581 cgtagtaaca agcatgctag ttttcagtaa attaaatctg aaaaaagctt tatcttctaa
     5641 ataaaaagac ccttggtagc tagattttta acctaactat caagggtctt tgtgtggaaa
     5701 aatcagtttt tattcgattt cttctaccgt ttcctgcaaa agctgttcat attttttgtt
     5761 tcctttatat aaatcaccaa tcgtccatgc caacaggaga aggacaagaa caatgattac
     5821 ataagcgatg tgatcagcca atgctagttg agcggaagtg gcttgctcgc cgaagaaact
     5881 ttcgatctgt cctggtaatc ctcgtaagtt caagtaagtt agactaatca cagagaacca
     5941 gccaagaagt tttacgatga atgaattttt aaatcggcta cccatctccg cttggctgtt
     6001 ggtcatcatc agtaaaggaa tcatcgaaaa tggcaacgca aaagccaaga acacctggga
     6061 ttcattcatt aagttattca aagcggcatg ttcttcgatc gttccttttg tacttgtaaa
     6121 caaaacacaa atcaatacag gaatcacgga aagcaatcgt gtgaccagtc ttcttagcca
     6181 aatcgggata cgcatatgga tgaatccttc catgatgact tgaccggtca acgtaccagt
     6241 aatagtagag ttctggccag aagcaagaag tgctacagca aataaagtgg acaagatacc
     6301 agattttgct acagcaatca aaataccatt gctcatagct gatggatcag ataaagcttc
     6361 atacagacca aaaaaggaag gatcttggac agaaccggtt ttgaagacag cgacacccat
     6421 gatcaatagc agtgcattga ctagaaaagc cattgacaac tggatattag aatcccaagt
     6481 agaaaaacgt acagcttgtg ctacatcttc ttcgttttgg tgatcgatct tccgagtttg
     6541 tgaaactgct gaatgaagat ataagttatg cggcatgact gttgcaccaa taatccctaa
     6601 agctccagtc agcggagttt cgccgccgat gaccggtgag tctgagaatg tatgactgtt
     6661 agggataaag ccagcaaata aactgtgcca atctgggtta gacaagccaa cttgatagac
     6721 aaaaacgaat aaaatgacga aaattaagca gacaaccagt gcttcgatct tacgaaaccc
     6781 gacttttgtc agtagtaaga gaacgaaaac atctaataca gtaataaaga cggcaatcac
     6841 caatggaata tcaaaaagta agtagagggc gattgctgca ccaatgactt ctgcgatgtc
     6901 tgttgccata atggctaatt cagtcaagat ccataaaatg acacccaatg tcttacttgt
     6961 ccgtgctcgg attgcttggg ccaaatccat ttgggtcacg attcctaatt ttgctgccat
     7021 atactgtaaa agcatagcaa ttagactaga catcaaaata actgacatca ataaatattg
     7081 gaaattttgt ccaccagtga tcgaagtgga ccagttacct ggatccatat aaccaactgc
     7141 tactaatgca cctggtccag agtaagcaag aagtgtttta aaaaaacttt ttccctttgg
     7201 tacatccact gttccattta cttcagctaa agataaacca tttgtatgtt caataagtcg
     7261 atgcttatga aattgtttgt tggattgatt attcaagaaa aaaccacctt tcctatttat
     7321 ctacgattac attctacacc tcagaaagaa aaaattaaag aaaaagattc ggtatgccta
     7381 attttttttc gtggtgtgtc ttgctggtta atgaaagaaa ttttgttctt ctctaaaaaa
     7441 accactattt tttgcatgaa actaaagaat acgcctttct tttttcaata aactttagta
     7501 aaataaatca ttgagagcaa ttcggaaata gaaaggagag atttatatgg tatcaacagg
     7561 tattgtcgta cttattgcta ttattgcttt gctaatcggc gcagtaggtg gctttttcct
     7621 agcgcgtaaa tatatgaaag actatttaga aaaaaatcct cccgttaatg aggacatgtt
     7681 gcgatcaatg atgatgtcaa tggggcaaaa accatctgag aagaaaattc gccagatgat
     7741 gcagcaaatg aagaaccaag ggaaaaaata ggttctgacc caaagacttt ttgcgggctg
     7801 attttttcag tccgtttttt atttcgtaat ttgttagact attcagacta ttcagcaaaa
     7861 aagacaagca cagacaatcc tatgctcttg ataaagacaa aggaggagta aaatgtcgat
     7921 ttttagaaaa ttaggttggt tttttcgtca ggaaaagaaa cattatttaa ttgggatttt
     7981 ctccctattc gcagtggcct tggtacagtt agttcctcca aaagtgattg ggatcatcat
     8041 tgatgagatc gccgataaga atttatcaat aaagatcatt tcctggtgga tcttattatt
     8101 gatccttgca gctttcttac aatatctttt tcgttatatc tggcggacga atatttgggg
     8161 aagtgcagct cggttggaga gagaacttcg gcagcagcta ttcaaacatt ttacagaaat
     8221 ggacaatctc ttttaccaaa aatatcggac tggagatttg atggctcatg caacgaatga
     8281 tttgaatgca atccagaatg tagcaggagc tggaatcttg acctttgcag attcagtgat
     8341 tacgggcgga acaacgatca ttgcgatgat tttgtttatt gattggcgct taacactgat
     8401 tgcgctgatt cctctgcctt tgctagccgt tacttcacga gtgctggggt caaaactcca
     8461 cgatgctttt cgtgattcgc aagaagcttt ttctagtatc aatgataaga cacaagaaag
     8521 tatcactggg atcaaagtca ttaaaacatt tggacaagaa aaagaagatc tcgcggattt
     8581 tgaagaaaag attgaagatg caattattaa aaacaaacga gtcaattttt tagactcctt
     8641 atttgatcca ttcattacct tgattatcgg tctgtcttat gttgcaacaa ttattttagg
     8701 tggaaagtat gtcttggaag gcacgatctc aatcgggcag atggtctcat ttattagcta
     8761 tatcggtatg cttgtttggc cgatgtttgc gattggtcga ttgttcaacg tattggaaag
     8821 aggaaatgct tcttatgatc gcgtcaatga acttttgcat gaaaagacgc atatcgtgga
     8881 acaactgggt gcagtgcaga ccccggcaaa aggcgagctg aatgtagcta tttcctcttt
     8941 taactatccg aaagaagaat caccagcatt agaacaaatc aaatttgcga tccaagaagg
     9001 agaaacatta ggtattgttg ggaaaactgg tgcgggaaaa acgacaatca tcaaattact
     9061 gatgcgtgaa tacgatgatt atcaagggaa aatccaattt ggcggacgtt cgatcaagga
     9121 atattcattg aatgctttat taggatcaat cggttatgtg cctcaggatc acttcctgtt
     9181 ttcgatgacc attcgagata atatccgttt ttctaatcca aaactttcag aagaagaagt
     9241 agagcatgcg gcttctttag catttatcaa caatgaaatc aaggaattgc caaaaggtta
     9301 tgatacctta gttggagaac gtggagtctc tttatctggc ggacagaaac agaggatttc
     9361 gattgctcga gcattgatca aaaatcctga attgctgatc ttagatgatg cactttctgc
     9421 agtagatgcc aaaacagaag aagctatctt gcataattta aaagaaataa gaaaagagaa
     9481 aacaaccatc attgcagctc accgtttaag cagcgtgatg catgcaaaag aaatcattgt
     9541 tgtcgatcaa gggaaaatca ttgaacgagg aacccaccac gagctgcttg cattaggtgg
     9601 ctggtatgaa cgaatgtggg aaaaacagca attagaagcg aagatagaag gaggcgaggc
     9661 ctgatggatg aaaattatca atcggaatgg acaaaatcga ttccacttaa agaacagaag
     9721 aagatcatca aacgactgat gaagttcgcc aaacctttta gaagaacgtt catcgttgct
     9781 atcttatttg cgtttgccct gtctgtgatc aatgtcctat tgccaaggat catccaaact
     9841 tttatggatg atcatctggc taaacagtca gctacgacac aagttatttt gttttttgct
     9901 ggtgtgtact tgtttggagt catagtcaaa agtatcattt ggtttttcca atggtatttg
     9961 tattccatgg cttcattgaa gacctatcag tatgttcggg taagattatt tgaaaagctg
    10021 catacactag gtatgcgtta ttttgaccag acaccggcag gatcgaccgt ttctcgagtg
    10081 acgaatgaca cagaaacgct atttgaattt tggtatgtct ttttgatggt attgactggg
    10141 atctttgcag tcgtttcttc tttcgttgcc atgtttcaaa tcaatggaga aattgccttg
    10201 tggactttgg tgttcttgcc gattttagga atcgtcatct ggtattacca gaaattcagt
    10261 tctcgtattt accgaaatat gcgggaaaaa ttaagtcagc tgaacacgaa attgaatgaa
    10321 tcgatttccg gcatgcgtat tatccagcag tttcgccaag aagctcgttt agctaaagaa
    10381 tttgaacaag tgaacaacga gtatttagac acgcgctatg cgatgatcaa aacgaattcg
    10441 attctgttaa gtccaatcat caattttctc tatgcattgg cgattgcgtt ggcgttgact
    10501 ttgtttggga tcgatgcgtt gcattctccc gtagaagttg gactgatcta tgcatttacg
    10561 acctatgtcc aggaattttt taatccaatg tctcaaatga tggacttttt gagcaccttt
    10621 acagatggga tggttgccgg tagtcgtatt ttgaaaatca tggatacaga agaatatgca
    10681 ccgcaacaaa atcctggagc agatcaagcg attacttcag gtaagatcga gtttcgcaat
    10741 gtaagctttt cttatgatgg agagcacaat gtattgaaca atatctcttt tgtcgctaat
    10801 ccgggtgaga ccgttgcatt agtggggcac accggaagcg gaaaaagctc gatcatcaac
    10861 gtattgatgc ggttttacga attttatgaa ggccaaatct tgattgatga tcatgacatt
    10921 cgagaatatc caatggaaga gttacgtaag aaaatgggat tggtcttgca agatgccttc
    10981 ttgttttatg gagatattgc aggaaatatc cggatgatga atccagctat cacagacgaa
    11041 cagattcgcc atgcagctga gtttgttcaa gcaaacaagt tcatcgatga attaccaaaa
    11101 ggatatcact caaaagtgat cgaacacggt gccagctatt ctagtggaca acgacagctg
    11161 atcacttttg cgcgaacaat cgtgacagat cctaaaatac tagtattgga tgaagcaaca
    11221 gcaaatatcg atactgaaac cgaaggattg atccaagaag gattggcaaa aatgcgacaa
    11281 ggacggacaa cgatagcgat tgcccatcgg ctgtccacga ttcgggatgc agaattgatt
    11341 ttagttttag acaaaggtca tatagtagag cgtgggaatc atgaaacact tctagcgcaa
    11401 aatggtttgt atgcagatat gtacaaactg caaagcagtg gtgtgtaaaa agaggttgtg
    11461 acagtagcat agtagcattt tgccctaagc gctagagtga aaataaaaca gagcttcgca
    11521 gattcttcat ctgtgagcgt tagtggcgta gtcacccgat ttaaaaaaga cgacgacagc
    11581 tagtcaaaag cggctaataa ccactaagaa agcgttgaca aaagcgtttg ctttcgataa
    11641 tataaagaaa acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt
    11701 ctcccatggt tccatggacg gctttttggg tttttctttt tgttttggat acagccctat
    11761 atatcatgga aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat
    11821 aaccagggat gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa
    11881 gaaagtgggt gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga
    11941 cgggctttca caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat
    12001 ttcttgtgaa atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt
    12061 gtatattgca ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt
    12121 tgtaccaaat gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg
    12181 gaaaaaaaac agtgaagaaa atctcggata aataccacat caagttatca gaagatgaag
    12241 ccggatttat cgcattgcat ttagtcaatg cacaaggcat tcaaagccaa caagacaata
    12301 cctacctgtt cactataaca atacaggata ttctaaatat cgtccgctat tactataaga
    12361 ttacatttga tgaagattca gtccattttt ttcgattcgt cacacactta cgtttttttc
    12421 tgacacgcgt cctagatgga gaaaaatcag aagtgggaga agtttctgag gaattgtttc
    12481 aggttattca aaaaaaatat aaggatacgt ttggttgcgt tgagaaaata gcagactttt
    12541 tagccgagac gttcaactac cagatgtcca cggacgaaaa aatgtattta acgatccata
    12601 ctgcgcgttt agtgcatgag aatcgttgta aataaataaa aaaaggagga taaattaaca
    12661 agaacgtatg aaataagggt agtgacattc agttggctaa accttccact aataaattgg
    12721 agggaataga aatgggaaaa taccaagaat tagcgagaaa aatcgtagaa aatgtcggcg
    12781 ggaaggaaaa tatcaatggt ttgacacatt gtatcacaag gcttcgtttc aaattaaaaa
    12841 acgaagaaaa agcaaatgat gagatcttga aaaatatgga cggtattgta acggtaatgc
    12901 gagcgggagg gcagtatcaa gttgtcatcg ggaatcatgt gccagtcgtg tttgaagagg
    12961 ttataaaagc aggaaacctt acttttgatg aggctgtttc aacagaaaaa atgagaccgt
    13021 ttgatatgct tattgatgga atatcgggct gtttccaacc attccttgct atcctagctg
    13081 ctgacgggat gattcgtgga ctgacagcat tccttgtctt cttaggcgcg tttgaccgcg
    13141 gttctggcac atttgttatg tttgataata tcggtgattc tgtattccaa tttatgcctg
    13201 ttattatcgg tttaacagct gcccgtaaat ttaaagtaaa tgaattcgta gggatgttgc
    13261 ttggcgcagc attaatgaat cctagcttgt cgctggaagc tttgagtggt gcagcagaag
    13321 caccactaac gaccatattt tctggcacta tttttgaagc acctatttat caaacagtat
    13381 tcggcattcc ctggattgcg cgtaactacg cttcttctgt cattcccatt atctttattg
    13441 tgttgttagc tagccaaatt cagaaaccaa ttaaaaagtt agtacctgag atgatcgcga
    13501 atttcttcgt tccattcttc accgttctta ttacaatgcc tttaggtttt ttactcgttg
    13561 gaccagtatt cacattcgca actgatattt taatggctgg atttgaaaca cttttagcac
    13621 tttcgccagt tatttatgga gcaatcgtag gcttcttctg gcaaattctg gtgatgtttg
    13681 gtctccattg ggcaatcgta ccaatgggat tgatgcaatt ttctgtgaat ggttggcaaa
    13741 atattatgac acctgttgca gtagtgtctt ttggacaaac tgcagcactt accgcactct
    13801 actttaagct tagaaatcca aaagacaaag caattgcaat accagccatt gtttctggta
    13861 ttgtggggat tacagagcca gctatttatg ggtttacatt gccacgtaaa aaaatattta
    13921 tttttacctg tgtcggtggt gcgttagggg gcgcgtatag tgggttaatg aatttaactt
    13981 cttggaatca aggtggacta ggcatcttta caattcccaa ctatattaga ccagatggtg
    14041 atttaacaga tgtgattaat gtattgattg ggatcgccat tgcgatggtg gtatcctttg
    14101 cgctgacttt tttcttttgg aaggaggaag cagatgaaac agatgataga cagaaaagat
    14161 caggtaaaga aattgtaaaa acaccgatac aaggacaaat cgctccactg aatgcagcaa
    14221 aggatgcagc ttttgcacaa ggaacactag gtcgaggaat cttgatctac cctgaaaaag
    14281 gagaagtacg tgcccctttt gacggaacag tcatgtccct gtttccgacg aaacatgcaa
    14341 tcgggatggt ttctgaagca ggtctggaat tgctgattca tgtcggattg gatactgttc
    14401 agttggaagg gaaatatttt gaaagcttgg tgcagcaagg agaaagagtg aaacaaggcg
    14461 atctgttgct tcgcttcgac aaaaagaaaa tcgaagagga aggatacagt ttagaaacac
    14521 ctgtgattgt catcaattac atggattatt tggatatttt ggaaaatcaa ggggaaacgg
    14581 tcgggccaga cgatgcactt atcaccgcac tgacctgaac ggttaggtga aagaatggac
    14641 taggcttaac tattcttttt agtccacttc tttattttca aaattgaaag gctctttcat
    14701 gatggctgag ctgatagggc gtttatcaaa tttttgtcct acagaaatta gatggactaa
    14761 attctgttga taattttctg caaaaacaca actatataac acgtctagaa gataacaaat
    14821 cgatgtattg atcgtaaaat taccaatttt cgaatacagt cgttcgcgtg tcgtaagtcg
    14881 caaagcggct gtactcattg aagatagcgt tgaatcaccg atactggtca aagctaaaat
    14941 tggcacatga ttttgtttca atatttctgc ggtacgaagg atcacattgt tttcacccgt
    15001 ataagaaatc agaatcccac acatatcact tgtgcaattg tacgcttcat agaacgattc
    15061 accaaaagtg gtgacaatcg tgacatccat tttgatgcgc ttcattttca gcatgaaatc
    15121 ttgagaaatc aaagtattcg agttactggc gaaaatcacg attttttttg cttgaagcat
    15181 tagttgtttg gctttttgca gttggtcatg atgaatgaga gaaagtgtat cttgaatcgt
    15241 catctgttct aaagaagcca ttttatttgc gatcgtcata ataccgtctg tggtggaaaa
    15301 aggaagattc gcatctacgt cttgaaaatt cccttcaaga taggctgttt cagctaaaaa
    15361 agcattacga aaactgaccc agccatcaaa acccattttt tttgctacac ggatgagtgt
    15421 ggacgggtgg acataacatc gctcagcaat ctcttggatg gtcatttcgc caagtgctgg
    15481 agaatcgctt aagagaaact gaatcacttc attttctgaa ggagaaaagg agatttcttt
    15541 gattttttca cgtaataaca tgtagatacc ttcttcttgt ttttttacgt aaattataac
    15601 ttttttgaaa atgatttgta aacggattta tgttatatag cttaataaag acattttttg
    15661 acaagatatt tacaaaaatc cagaagaaaa cactaacttt tgtaaatgtt actggaaaga
    15721 aacgaatttc tattgtttgt tttgtttaca aaaaagataa taaaagtgta aaaagtaaag
    15781 gagtgagaac atggcaaaag gaatcaaaat cgctacgatt ggtggaggca gcagttacac
    15841 ccccgagttg atggaaggat ttatcaaaag atatgaggaa ttaccgatac gagaaatctg
    15901 gctcgtagat gtagaagaag gaaaagaaaa gcttgagatc gtcagtcaga tgtctcagcg
    15961 tatgtgggat gcttctccgt atgaagtcaa aatctatgca actctggatc gggaagaagc
    16021 attgaaagat gcggattttg ttacgactca gtttcgtgtc ggtcaattag atgcacgaat
    16081 caaagacgag cggatcccta gttattatgg gatgattggg caagaaacaa atggtgctgg
    16141 cgggatattc aaagcttttc gtaccgttcc agtggttttg gcaatcgttg aagacatgaa
    16201 gcgtttatgt ccaaatgctt ggctgattaa tttcgcgaac ccaagcggga tggtgacaga
    16261 agcagttatc agatatggca aatgggataa agtgatcgga ttatgcaatg tgccagtaat
    16321 ggcgatgatg acagaaccaa caatgcttgg caagaaaaaa gaagagctga tctacaagtt
    16381 tgccggtcta aatcacttcc attggcacaa agtagcagat aataaaggaa atgaccgcac
    16441 ggcagaattg attgatcgtt tatacgatga agacaatgga ttgccgaaaa atatctttga
    16501 catcccgttt ttcaaagaac aattaaaaca aatgaacatg atcccttgtg gctaccaccg
    16561 ctattactat cgtgaagaag aaatgctgga acatgcttta gaagaatatc aaacgattgg
    16621 tacaagagcg cagcaagtga aagaaacaga agcagagctg tttgaattat acaaagatcc
    16681 agaattggat cataaacctg aacaattgca aaaacgaggt ggtgcttatt attcagatgc
    16741 tgcctgtgaa acgattgctt cgatctatgg aaataaagaa acacagatcg tggtatctac
    16801 aaagaatgat ggagctgttc ctgatctgcc ggcagattgt gtagtagaag tcactgccta
    16861 tgttggcgca caaggcgcac gaaatgtagc gtttggttcc cttcctgctg ctgaaagagg
    16921 ctggttgcaa gtaatgaaga atatggaatt gctgacgatc gaagcagccg tgactggtga
    16981 ttatggaaca gctttgcaag cgtttacgat caatccgctt atacgatcag gaaagacagc
    17041 aaaacggatc atggatgagt tgtttattgc acatcagcag tatttaccgc aattcaagga
    17101 aacaatcagc cggttaaaag cagaaggaat cgaagtccaa gatgaactgg caagagaatt
    17161 atcaagcaat acacatgtaa tggaggaagt atgatgggat tcagaaaaga ttttttatgg
    17221 ggcggagcta cagcagccaa tcaatgcgag ggcggttatg atgaaggcgg acgtgggtta
    17281 gcgaacgtag atgttgtgcc aatcggagaa gaccgttttc ctgtcattac tggaaaaaag
    17341 aaaatgtttg aatttgatga aaatcatttt tatccagcaa aaattgcgat cgatatgtat
    17401 catcattata aggaagatat tgctttattt gcagaaatgg ggttcaagac ttatcggatg
    17461 tccattgctt ggagtcgtat ttttccaatg ggcgatgaaa aagagccaaa tgaatctggg
    17521 ttgaaatttt atgaggatgt ctttaaagaa tgtcaaaagt acgggatcga gccgcttgtg
    17581 acgattacac attttgattg tccgatgcat ttgatcaaag aatatggtgc gtggcgttcc
    17641 agagaactcg ttggttttta tgaaaatctg tgccgagtca tcttcaatcg ctataaagga
    17701 cttgtcaaat attggttgac attcaatgag atcaatatga tccttcatgc accattcatg
    17761 ggcgcagggc tgtattttga agaaggagaa aatgaagaac aaatcaaata tcaagcggca
    17821 catcatgagt tggttgcaag tgccatcgca acgaagatcg ctcatgaagt agatccggag
    17881 aacaaagtag gctgtatgct ggcagcagga gcatattatc catacacttg caatcctgaa
    17941 gatgtttggg cttctagaaa gtcagatcga gaaaattatt tcttcattga tgtccagtca
    18001 cgaggagaat atcccgctta tgcattgaaa gaaatggaac gaaatggcat tcagatcaaa
    18061 atggaacctg gggatgccgc attgctgaaa gaacatactg ttgattttat ctccttttct
    18121 tattattctt ctcgagtttc gactactgac aacgaactct tggaacagac tgcaggaaat
    18181 atctttgctt ctgtcaaaaa tccatatctg gaagcaagtg aatggggttg gcaaattgat
    18241 ccattaggct tgagaataac gatgaatgat atttatgatc gttatcaaaa accactattt
    18301 attgtagaaa acggcttagg tgcagtcgat acgcccgatg agaatggtta tgtggaagat
    18361 gattatcgaa tcgactatct ggcagcacat atccaggcaa tgaaagatgc agtagaatta
    18421 gatggagtcg atctattagg ctatacgact tggggatgta ttgatttagt atcagctggt
    18481 acaggtgaga tgaagaaaag gtacggattc atttatgtcg atcgtgacaa cgaaggaaat
    18541 ggcacattag cccgttcgaa gaagaaatca ttcaactggt ataaaaaagt aattgattca
    18601 aatggagaaa atctttcgaa cgattgatca atgatttata aaagtctatt gtttttcagc
    18661 ggatttttcg agtataataa agctattcga gaaacgcaag cagaacaata gacttttttt
    18721 cgcattggct gtaaagtatg cttacttgat taagtaaagg agaatctaca tatgtatgat
    18781 gtaatcgtta ttggcgcagg gcctgctggg atgactgctg ctttatatgc ttcacgttca
    18841 aacttatccg tattgatgat cgaacaaggc gctcctggcg gccaaatgaa caatacggca
    18901 gaaatcgaaa actatcctgg tttcgattca atcatgggtc ctgatctagc ttataagatg
    18961 tatgaaggtg tttcaagatt tgggacagaa aatgcttatg gaatcgtgca agatatcaag
    19021 gatcacggca gccacaaaga agttatttgt gaagataaaa cttacgaagg aaaaacagtc
    19081 attatcgcaa caggctgtat ccaccgtaaa ttaggcgtac ctggcgagga agaatttgct
    19141 ggacgtggcg tttcttactg tgcagtttgt gatggtgcat ttttccgtaa caaacgtcta
    19201 gtagtgatcg gaggaggaga ctcagcagta gaagaagcca tctatttgac acaatttgct
    19261 tctgaagtag tcatcgtcca ccgtagagat gaattacgtg cacaaaagat catccaagac
    19321 cgtgcatttg cgaatgaaaa aatttccttt atgtgggata cagtcgttga cgaaatcgtt
    19381 ggaaatgaaa tggtagtgac aggtgtgaaa gcacgtaatg tcaaaactgg caatgagttt
    19441 gatttggcag cagacggagt attcatctat gtaggattgg atccattaac tgcaccattt
    19501 agagaagctg ggttgacgaa cgaagccggc tggattccaa cagacaacga aatgaagacg
    19561 aatataccag gcgtttttgc catcggagat gttcgtgaaa aagatttgcg acaaatcact
    19621 actgctgttg gtgatggtgg gattgctgga caacaagtgt ataaatactt ggaatcttta
    19681 gaagaaacaa ctgcataagt ttaaaaagag tgaaagaacg gtttcaattg ttcttttact
    19741 cttttttttg cgtgaaatct actgaatccg atttatgcga atacttgtaa ccgtgcttga
    19801 taaaagaatc ttttcttgat ttaagcgttt tttggtcctc atactttcat atctaataag
    19861 tgaatgctat acttattaag aaccatattt cagaaagagg tgttattttc atgtcttggg
    19921 aacaagtgta tgaacaatgg gtacatgaag aaaatttaga agaaaactta aaaaaacaat
    19981 taaatgatct gggtgaagat cctgaaaaac ttgaagatgc tttttatgca ccgttggaat
    20041 tcggtactgc aggcatgcgg ggaatcttag gacctgggat caatcgaatg aacatctata
    20101 cggtaagaca agcaacagaa ggacttgcac gtttcatgga tacacaaggt gaagaaacaa
    20161 aacgccgagg tgtcgctatc gcttatgact cacgccatca atcaccagaa tttgccatgg
    20221 aagcagccaa aacattagca catcacaata tcccttctta tgtattcgaa agtttacgtc
    20281 caacaccaga gctttctttt gcagtccgtc atttgaagac gtttgcggga atcatggtaa
    20341 ctgcatccca caatccagca gcttacaacg gatataaagt ctacggagaa gatgggggac
    20401 aaatgcctcc tgcagacgca gatgcattga cgaaatatgt ccgtgaagtc agcaatccgt
    20461 taaaagttga agtattatct gacgacgaag ctgaacatag cggattgatc acgatcatcg
    20521 gagaagaagt ggatgccgct tatctagaag aaatcaaagc agtaacgatt gatcatgaat
    20581 tggtggaaac aatgggtaaa gatttgaagc ttgtgtacac accacttcac ggaacaggaa
    20641 aaatgttggg cgaacgtgcg ttgaaacaag ctggattcga acaattcgta ctagttccag
    20701 aacaagctgt cgcagacccg gatttcacta cagtcaaatc accgaatcca gaagaacact
    20761 ctgctttcga gtatgcgatg cgacttggcg agaaagaagg cgctgactta ctgattgcaa
    20821 cggatcctga tgcagaccgt ttaggcgcag ccgttcgttt gccaaatggc tcctatgaag
    20881 tattgacagg aaaccaaatc ggagccctgt ttgctcaata tattttggaa gcacaccaat
    20941 cgcgaggcac tttacctgaa aatgcagcgg tattgaaatc aatcgtttct agcgaattgc
    21001 ctacagcaat tgcggaacac tatggggcta ccatgttcaa tgtattgaca ggattcaaat
    21061 tcattgctga aaaaattcaa caatttgaag aagatcattc ccatactttt atgttcggat
    21121 tcgaagaaag ttacggttat ttagttaaac ctttcgttcg cgacaaagac gcgattcaag
    21181 cattgctctt attagcagaa gtagcagctt actataaaaa acaaggcaaa acattgtatg
    21241 acggcttgca aacgattttt gaaacatatg gctactacgc agaaaaaacg atttctgtga
    21301 caatgagcgg acaagaaggc tctgcaaaaa tctctgcttt gatggaaaaa ttccgtgaag
    21361 aagcaccgac agaatttgcc ggagcaaaag tcatccaaac agaagacttc aaagagttga
    21421 cgagaacaga ccgtgaagga aacgtagaaa aactaacgac accgccatct gacgtattga
    21481 agtatacgct ggaagaaaat ggctggatcg ctatccgtcc ttcaggaact gagccgaaaa
    21541 tcaaatttta tatcggtgta aaaggaaact cttcagaaga cgcagctgaa aaagtagccg
    21601 cttatgaagc gattatcaaa aaaatgacag aagaataaaa aaacttgaga ctgatggaaa
    21661 aagtggtaaa ctttagaagt cagtcttttt tcaagttgga ggtgaatgga ttgccagaca
    21721 cgcaaaaaac aaaagtagag aaagtcagcc aactattcaa aatgttgagt gaccccactc
    21781 gtctcaaaat tctgctgtat ctgaaagacg gggaacaaaa tgtgacagca atcacccaag
    21841 cagtagaaat ggaacaatct gctgtatcgc atcaactgcg tttattaaga gaaaaccatg
    21901 tcgtcaagtc ccatcgtgag ggaaaagcga ttttgtatag tcttgatgat tctcacgtac
    21961 tggatatttt gaaccagacg ctcaaacatg tggaacatca ataacgatag gtgcaaatag
    22021 agacaaactg gactgtatca acgaatcttc ttcgctgaca cagtccagtt tttttaagaa
    22081 aaagacaaga aaaactgtta gtctgtttct tcggtggttt cagttccata ataactatga
    22141 tcttctaggt ctaaagcatt caaaataagt gaagctgttt cttcgataga taaagaagcg
    22201 acattgatta ctacgcagcc caatttttca taaagatcat tcgcaaaatc gagttcagct
    22261 cgtatttttt cgatatcgga ataagcagta tcaggattta atccgtaaga gcgcatccgt
    22321 tctttacgga tattgtttaa aatatctggg ttattcgtca gtcccacgat ttttttagga
    22381 tcgatctccc aaagctgttt gggaatatgt gcttgtggga tcaaaggtag attggcaacc
    22441 tttaaatttt tgttagctaa aaacaagctc aaaggcgttt tggaagttct ggatacgcct
    22501 agcaaaacga catctgcttc tagaaaccca cgaggatctt tcccgtcatc gtattttaca
    22561 gcaaattcca ttgctttgat ccgtttgaaa tagttctcat tcaataaatg aagggcgcca
    22621 ggttgccgaa tgggcttgat accagttaag cgctcgattt ctgatatcgg cggagtcatc
    22681 acatcaaaac taaataattg atgttcttca caaaaagaac gcgtcatctc gatcaattct
    22741 tgattgatca atgtgtgtag gacaagggct ttttctttta gtgcatcttg cagtgcctgc
    22801 atcaatgttt tctcatctgt cacaaatgtt ctgcggaata aattgaactc aacggtagga
    22861 tattgggcca tagtggcttg cgctaatttt gtcgcagttt ctccagcaga gtcagagatg
    22921 acaaaaaaag taatgatttc tgtagaatcg gaagacatgg ataaaacctc acttgcattt
    22981 tttttatgtt tattatacca tactctagaa tgtgtacttc gtaatcattt tgatttggag
    23041 gaataagtca tgaaaaaaca gacattgatt gaacaatcac tagaaacatt gaaaaataac
    23101 ggttttaaat atactaaaaa aagagaagca ttgttaactt atttagtgaa gcgcaatcgc
    23161 tacgtatccg ctaaagaagt ttttgactat atgaatcagg aattcaaagg agtaagttac
    23221 gatacgattt atcgaaatct ccatgatttt gcagaaatcg atttactgga agaaaccgat
    23281 ttaaatggag agatgaagtt tcgttttcat tgctgtcaag gggaaatcgg ccatcaccat
    23341 cactttatct gtacggtgtg cggaacaacg aaagaaatcc atatgtgtcc aatgaattat
    23401 ttcgaagaac agctcggtgg atgtacgatc gaagggcata gattcgagat tttcggaaga
    23461 tgtgaaaatt gtcaataagg atttaaataa aataacattt gataatttta aaaaaaagca
    23521 agatttcctt gacggtcaaa acagcattag atataatatt tacaggacag tcttgttttt
    23581 tgagatatat ttcaataaca attacggtta ctttttaagc tcggagggag ggaattaaca
    23641 tgtcaaaaac agtcgttcgc aaaaacgaat cacttgacga tgctcttcgt cgcttcaaac
    23701 gttccgtgtc aaaagcgggt actttacaag aatctcgtaa acgtgaattc tacgaaaaac
    23761 caagtgtaag acgcaagaaa aaatctgaag cagctagaaa acgcaaaaaa ttctagttct
    23821 gtgatgaatt ggagttggta gtgtgtcact actaagtaga ttaaacgatg acatgaaaac
    23881 agcgatgaaa gcgaaagata aggaatcttt acaagtcatc cgaatgatca agtcatctat
    23941 acaaaacgag cagattaaag aaggtcacga cttgacagaa gaagaagaat taacggtgtt
    24001 gtctcgcgaa atgaaacagc gacgtgattc tttgcatgag tttgaagaag caggacgtga
    24061 cgatttagct gaaaaagtaa aaagtgaaat tgtgatagtc gaaaaataca tgccggaaca
    24121 actttccgac gaagaaattc gacaacttgt tcaagaagca atcacacaaa ctggtgcttc
    24181 atcaatgaaa gaatttggca aagtcatggg tgcgatcatg cctaaagtca aaggaaaagc
    24241 agatggaaac caagtaaatg ccattgtgaa agaactatta caatcttaat tttatcattt
    24301 aaactgcgga caatcattgt ccgcagtttt tttgtgcgcg taaaaaagag aacta
//
LOCUS       DAFEPQ010000038        23659 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.038, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000038
VERSION     DAFEPQ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.038"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            257..532
                     /locus_tag="LP543_001637"
     CDS             257..532
                     /locus_tag="LP543_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321805.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2623
                     /locus_tag="LP543_001638"
     CDS             545..2623
                     /locus_tag="LP543_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBL2321806.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2783..4669
                     /locus_tag="LP543_001639"
     CDS             2783..4669
                     /locus_tag="LP543_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL2321807.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4681..5628
                     /locus_tag="LP543_001640"
     CDS             4681..5628
                     /locus_tag="LP543_001640"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBL2321808.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5648..6175
                     /locus_tag="LP543_001641"
     CDS             5648..6175
                     /locus_tag="LP543_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL2321809.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6238..6891)
                     /locus_tag="LP543_001642"
     CDS             complement(6238..6891)
                     /locus_tag="LP543_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBL2321810.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7023..7865
                     /locus_tag="LP543_001643"
     CDS             7023..7865
                     /locus_tag="LP543_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL2321811.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8023..8919
                     /locus_tag="LP543_001644"
     CDS             8023..8919
                     /locus_tag="LP543_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL2321812.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8922..9635
                     /locus_tag="LP543_001645"
     CDS             8922..9635
                     /locus_tag="LP543_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBL2321813.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9655..10173
                     /gene="msrA"
                     /locus_tag="LP543_001646"
     CDS             9655..10173
                     /gene="msrA"
                     /locus_tag="LP543_001646"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL2321814.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10170..10397
                     /locus_tag="LP543_001647"
     CDS             10170..10397
                     /locus_tag="LP543_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBL2321815.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10549..11100
                     /gene="lepB"
                     /locus_tag="LP543_001648"
     CDS             10549..11100
                     /gene="lepB"
                     /locus_tag="LP543_001648"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL2321816.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11183..11683)
                     /locus_tag="LP543_001649"
     CDS             complement(11183..11683)
                     /locus_tag="LP543_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBL2321817.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11962..12360)
                     /locus_tag="LP543_001650"
     CDS             complement(11962..12360)
                     /locus_tag="LP543_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL2321818.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12620..13480
                     /gene="ylqF"
                     /locus_tag="LP543_001651"
     CDS             12620..13480
                     /gene="ylqF"
                     /locus_tag="LP543_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBL2321819.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13473..14240
                     /locus_tag="LP543_001652"
     CDS             13473..14240
                     /locus_tag="LP543_001652"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBL2321820.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14296..15153
                     /gene="dprA"
                     /locus_tag="LP543_001653"
     CDS             14296..15153
                     /gene="dprA"
                     /locus_tag="LP543_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBL2321821.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15275..17353
                     /gene="topA"
                     /locus_tag="LP543_001654"
     CDS             15275..17353
                     /gene="topA"
                     /locus_tag="LP543_001654"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBL2321822.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17322..18698
                     /gene="trmFO"
                     /locus_tag="LP543_001655"
     CDS             17322..18698
                     /gene="trmFO"
                     /locus_tag="LP543_001655"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBL2321823.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18896..19801
                     /gene="xerC"
                     /locus_tag="LP543_001656"
     CDS             18896..19801
                     /gene="xerC"
                     /locus_tag="LP543_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBL2321824.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19838..20386
                     /gene="hslV"
                     /locus_tag="LP543_001657"
     CDS             19838..20386
                     /gene="hslV"
                     /locus_tag="LP543_001657"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBL2321825.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20400..21800
                     /gene="hslU"
                     /locus_tag="LP543_001658"
     CDS             20400..21800
                     /gene="hslU"
                     /locus_tag="LP543_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBL2321826.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21818..22609
                     /gene="codY"
                     /locus_tag="LP543_001659"
     CDS             21818..22609
                     /gene="codY"
                     /locus_tag="LP543_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBL2321827.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22691..23566
                     /locus_tag="LP543_001660"
     CDS             22691..23566
                     /locus_tag="LP543_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBL2321828.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8192 a         4079 c         4983 g         6405 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaaacgag tccaataaaa taaaaaggta aaaattttca
     2641 agttttataa aaaacaaaac atatcagaac aggatcgaat cggtcctgtt ctttttttga
     2701 gaacacaaaa agaacaaata ccaaaaaaat tttcctgaag ttcactattt ttatgataca
     2761 atatccaaga taaggaggga acatgttgaa agaattaaaa gtaactgacc tcaaaaaaac
     2821 ttatggtgaa aaagatttgt ttgaccagat ttcttttttg gtccacgaaa aagaccgtat
     2881 cgggttgatt gggacgaacg ggacgggtaa aacaagtctc ttaaatatta ttgccggtat
     2941 cgatagcagc gacggtgatc ggcaaacagt gttttatcca actgattacc gtattggtta
     3001 tctttctcaa gcagctgaat tttccgatga attaactgtt ctccaagcag tctttcaagg
     3061 aaacagcccc ttgatccaaa ctgttcgagc atatgaggaa gcattgatcg aactagggga
     3121 aaacggagaa gaccctgatg tgcaaaaacg ttatgctaaa gcagaagaac aaatgaataa
     3181 agaagatgct tggactacag ataccaacgc aaagattatt ttgcaaaaat taggaatcag
     3241 tgaattagat aaaaaaatca gtactttatc aggtggtcag aaaaaacgag ttagtctggc
     3301 gcaagtgttg attgatgaac ccgatttact attactagac gaaccaacaa accatttaga
     3361 ttatgaagcg atcgagtggt tggaaaacta tttgaaacaa taccgtggcg cactattgat
     3421 ggttacccac gatcgttatt ttctagatag agtagcaaac cgaatcttcg aactttcatt
     3481 tggaaaatta tacgagtata aaggaaatta tgaagcttac gtcttagaaa aagcagaacg
     3541 tgatcgtgta gaagtagaac aagaagaaaa gcgcaagcgg ctttataaac aggaattagc
     3601 ttggatgagg gctggcgtac aggcacgtgg caccaagcag caagcacgga tcaaccgttt
     3661 cgaagatcta aaggagaact tgtaccaagt aaaccaagaa gatgatttag agctgaacct
     3721 agcgacacaa cgtttaggga aaaaagtact ggaaatcaaa gatggcagtt accgtataaa
     3781 tgaccaaaca ctgctagaac atttagattt attgattcaa tctcgggaaa gactaggtat
     3841 tactggtaaa aatggtgcgg gaaaatcgac attgcttaat attctagctg gcagaattcc
     3901 cctggatagc ggtacaatgt ctatcggtga aacggttcat cttgcgtatt atacacaaga
     3961 aaatgaagaa atggcaccag ataaacggat gattgcttac ttacaagaag ctgctgaaga
     4021 agcaaaaaca gcagatggca gtcaaatcgg agtagcagaa ttacttgaac gatttttatt
     4081 tccaagattt atgcatggga cactgatagg caaactttca ggcggagaaa aacgccggtt
     4141 atttttgttg aaactgctga ttcagcaacc taatgtttta ttattggatg aaccgaccaa
     4201 tgatttggac atagcaacat tgacgatttt ggaagattat tttagaagtt ttccaggtgc
     4261 tgtgatcact gtttcccatg accgttattt cttggacaag gtagcagata agttattagt
     4321 attccaagga aacggaaaac aagaacttta ttacggaaac atgtccagtt atttactgaa
     4381 gcaaaaagag acccagcagc ctgctgaaaa agcgaaacca aaaatacaga gcaaggaacc
     4441 tgctgaaaag aagaaattat cttacatgga acaaaaagaa tgggaaacga ttgaagatga
     4501 gattgcagaa cttgaagaaa aaatctcatt gctccaagaa gagatgaatc atcaaggaga
     4561 taatttcact cgattacaag aattgcaaaa cgacgtatct gaaacagaag cacaacttga
     4621 agaaaaaatg gcacggtggg aatacttgag tgaatgggta gaagattagg aggaaaaagg
     4681 atggaacaag cgtatcttga cttaggaaga aaattgttgg aagaagggca tcgtaaagaa
     4741 gacagaacag gcacaggcac attaagctta ttcggatacc aaatgcggtt tgatctgcaa
     4801 aaagggtttc ctttgttaac aacgaaacga gtaccttttg gattgatcaa aagcgaattg
     4861 ctttggtttt taaaaggaga tacgaatatc cgctatctat tacaaaataa aaaccatatt
     4921 tgggatgaat gggcatttga acgctacatc aaaagtccag actataatgg accagatatg
     4981 actgattttg gccatagatg tttgacagat cctgaattca atgaagaata taaaaagcag
     5041 agcaagcttt tttgcgagaa gatcctaacc gatgattcct ttgctgaaac atacggcgat
     5101 cttggacaca tctacggcta tcaatggcgt cactgggaaa caaaagacgg tggctttatc
     5161 gatcaaatca aacaagtgat cgaagcaatc aaaaagactc ctgattctag aagattgatc
     5221 gtttctgcat ggaatccgga agatgttcct agcatggctt tgcctccttg tcatacgatg
     5281 tttcaatttt atgttcaaga gggacggcta agctgccaat tgtatcaacg cagtggggat
     5341 gtgtttctag gcgttccgtt caacattgca agttatgcgt tgctgactca cctaattgca
     5401 catgaaacag gtttagcggt tggcgaattt gttcacacat taggagatgc tcatctttat
     5461 atcaatcata tcgaacaaat gaaagaacaa ttgagccgag aaatacgcgc atttccgact
     5521 ttagttttga atcaggaaaa gcagtcagta tttgattttg agatggaaga tatccaatta
     5581 gaaggatatg acccacatcc ggcgatcaaa gctcctatcg ctgtttagaa tcagaaagga
     5641 tgagaaaatg ttagctgcaa tttgggcaca ggatgaaaac ggattgattg gaaaagaaga
     5701 ccgtcttcct tggcgtttgc caaatgatct gaaattcttc aaacaaatga cagaagcaaa
     5761 tacactagtg atgggacgaa aaacattcga agggatgggt agccgtccgt tgccgaatcg
     5821 tcagacgatt gttttgacga gagattcttc ctataaagca gaaggcgtgc atgtcatgca
     5881 tagtgtggaa gacgtgcttg ctctggaaaa agaaacagac ggtatctttt tcatcgctgg
     5941 agggtcagct gtttatcaag aatttctgcc attttgtacg atattatacc gtacagtcat
     6001 ccatcatgta ttcgatggag atgcttattt tccacctgtt gactggtctg attggtcact
     6061 gatcaatact agtcaaggag aaatggatga aaaaaatcgt tatccgcatc aatttgaaac
     6121 gtatcagcgt aatgagaatc ctgtaaaaag cgggacaggc gaattgacga tataaaaaag
     6181 ctatggatag actagctgtt atttagagct gtcccatcca tagctttatt ttttttatta
     6241 agtaaagaat aaaacagaga agtacataaa acctgcacct aacataacaa acaaatgcca
     6301 aacaacatgc atatagcgga tagaattcaa gctataaaag aatgcgccta tcgtgtaaga
     6361 aagcccgcct gctaccatca aaataacacc agtcattcca agagagtgat aaagaggaac
     6421 agcagccacg acacagagcc accccataaa aatatagatg atcgtagata cttttgatac
     6481 tctctcccgt ttatgcaacg tcaacgcttt atatataatg ccaagaatcg caaacaacca
     6541 gattaataca aataaagtcc aacctagcca tcctcggata ctcagcaaac aaaaaggagt
     6601 ataacttcct gcaatcaaaa gaaaaatcga actatgatcg aatacttgga acaccttttt
     6661 tgcttttgta aaaatcaatg aatgaaacaa agtagacgtt aaaaataaaa gaatcatcat
     6721 cgatccatag atagcataag agacaatatg gagggaggaa tgcaagtggg caccttttac
     6781 taaaagaacc actagtcccg cgatactcaa tccagtccca attccatggg tgactgcatt
     6841 aaacacttca tttaatattt catacgttcg attaaatttc gttttttcca tgaaaacggc
     6901 tcctttacta ggtgtaatta atgaaaaatc attatttgtc tgttatacta aaaatagaaa
     6961 ttgtcttaca tgtagtttaa catgaataag gcatcttgtt aaagaaagag agagagaaaa
     7021 taatgacaaa tgtaaaaatc gtaacagatt cttcatgtac gatggaaaaa aatgtacgcg
     7081 atgctttagg cattcgtgtc atgccgctat ccatcatgat cgacggaaca gtatatcccg
     7141 atgatgacca actagacggc gaaagattta tggaaatgat ggcgaaagca aaaaatctgc
     7201 ccaaaacaag ccaaccacca attggagaat tcatctcgat gtatgatgag ctaggagaag
     7261 acggcagtga agtgatttcg atccatatga caaaaggctt gagtggaaca gtagaagcag
     7321 caagacaagc cagtaatcta acaaaaacga aagtaacggt tatcgacagt gataccactg
     7381 accaaggatt atcttttcag gtaattcgag cagctgaatt agcaaaagaa ggtaaaacag
     7441 tagagaaaat ccttgaagaa gtcaaaaaag tacaagacaa tacaaaatta tacatcggaa
     7501 tatccacact agataacttg gtaaaaggtg gaagaatcag ccgaacaact ggtttgctgt
     7561 ctagtgtttt gaacatacgc gtggtgatga atttcgccca ttctgaatta atccctgtaa
     7621 ctaaggggcg aggaaagaaa acgttcgata aatggtttaa taatttgaaa aatgaactga
     7681 aagaattgcc aaatgtgcga caaatcggta tttcacatgc ggatgcaaaa gaattggcgc
     7741 aagaattcaa agagggtttg caagaattat tcccggacat gcatatccct gtactgcata
     7801 cgaatccggt tatcgctacg catactggta aagacgcgtt tgcaatcatg tattatgtag
     7861 attagtcgaa aagaggataa gccctcagcc tgaaaggctt gcagcttatt ctctttttct
     7921 ttataaaatt aggaaaataa ttgttttctt ttttcataaa gactaccttt ttgataaaat
     7981 ttttgataac attagaacca atcgaataca agaggtgaaa aaatgaaatt aacgaaacga
     8041 catttgatcc tcgttttgtt cccattatta attgcgatca tcatctatgc aggtttagct
     8101 gcttctatac cgaaagctgg cccactaatg aaaccagtaa ctactgtgag agaagatcca
     8161 aataaaaaag aaaaacttca ctacatagct atcggagatt cgctgactga aggagtagga
     8221 gatcaaacca agcaaggagg attcgttccc cttgtcgcaa atgatctaaa agaccgttat
     8281 aatttgaccg cggttgaaac agaaaactat ggtgtaaatg gtgaacgaag tgaccagatc
     8341 ttaaagcgag taaaaacaaa agaagaaatt cgaaacaata tcaaaacagc tgattttatc
     8401 acactgactg ttggcggtaa tgatttgatg aaagttatcc aaaataattt attcgggtta
     8461 acggtcaatt cctttaaaaa gccactgaaa aactatcagg aaaatgtcac agaactattg
     8521 gaagaatttc gatccctcaa taaaaccgca cctatttaca ttttaggcat ctacaatcca
     8581 ttttatttga actttcctga gatcactgac atgcagacaa ttgttaacaa ttggaacaaa
     8641 ggaacagaaa cggtgacaaa aaaaatgccg aactgctact ttatcccaat caatgatttg
     8701 ctttatcaag gattgaatca ggaagtaggt gtcacagaag aaggaacaga tcaaacaact
     8761 agcacgacag aaaatctaaa tggcatcaaa aataatgtgc tctatgaaga agatcatttc
     8821 catccaaata atttaggtta tcaattgatg gcaaatgcag tgagagatca gctgatcgag
     8881 acacaaaacc aatggttaga gaaggaggat gatcaataaa catgaaaaca agatctgacg
     8941 tatcttcaaa caaaacaaag aaaacaaatt actggaaatt cgccttcctt atccttcttg
     9001 gggtagtact tggttcgacc gtatttttag gcagccgtat ttttgctaat cgggaaccag
     9061 aactcccaga aataccagct cttacggaaa gacaaggaga tcctgtctta acgatcaatt
     9121 caaataaaga aaaagtcaat caaatcatca gctttttcct tagtgagtat caaaaagata
     9181 gtgatattga atataaattt tatttggaaa atgaagcact actcaatggt actttcgaag
     9241 tactaggttt tcctatcgat ttctatttat attttgatcc gtatgtgatg gaaaacggaa
     9301 atgtccaact aaaagctaga agtctttcta tcggaacatt gagtttgcca atcaaagatg
     9361 tgatgaatat gatcaagaga aactataaat taccagattg gatcgaagtc aatacagatg
     9421 atctgactat catgcttagg ttggatcaat tccgaatgca aaacgggatg tatattaaag
     9481 cagataaaat cgacttagta aatgatgaca ttcggtttag tctatatttg ccaaaagata
     9541 cctctgatgc ttcaaacaac tcaaatacca acaaaactag caccagcagt actgaaagca
     9601 agacaaaaga aaataaaaaa tcatcggatg agtagtatta gagggggaat cataatggaa
     9661 cgagcaatat ttgcaggagg ctgcttctgg tgtatgatcc agcctttcga cacgcttcct
     9721 ggtatccata cagtgatgtc aggatatact ggagggcatg taccagatcc gacttatgaa
     9781 caagtaaaaa gccaaacgac tggacataca gaagcagtgg agattatcta tgatccaaca
     9841 ttgatttcat atgaacaatt agtagaactt tactggcagc aaactgatcc aactgacgct
     9901 ttcggacaat tcgaggatcg cggtgacaat tatcgcccgg tgatctttta tttgacagaa
     9961 gaacagcgga tcattgctga aaaaagtaag aaacagttag ctgaaagtgg acgtttcgaa
    10021 cagccaatcg ttacaaaaat cgaaccagcc caaccatttt atctagcaga agaagaccat
    10081 caagagtatt ataaaaagca tcctgaaaat tttaaaagaa accatgcacg ccgggctgcc
    10141 tttatcgaag aaaattggag gaacgaaggg tgaatcgaag cttttttcat tatttaatga
    10201 cactgcgagg cggtggcaca catgatgctt tatctgtttt cgctacagaa gccggtaaag
    10261 acatccaatt tcctaagcac agctctagct acgaagaaat ttctaattat ctggaactga
    10321 atgtcgacta tttgccgtcc atggatatct ttgacgaagc atgggaaaaa tatttagaaa
    10381 ataatcatga catataagag atagttttgt actttatgat caaaaaagtt ttttgtccaa
    10441 tagcctaatt gaaagaatta tttttatttc agctatctag tcgcattttt ttgatatttt
    10501 tcttataata aagaaagaat gaaaaaaaga gggatggatt gatagagatt gacaaaaaaa
    10561 caacgctata ttaatcgctt ctggcttgta ttcaaatact tacttgtttc tgtattcctt
    10621 gcgttcatgt tgcggggatt tttattcatc ccggtaccag tggaaggcaa ttcaatggaa
    10681 aacgtgttga agcaaggtga tatggttgtg atggaaaaat tttcggagat cagacgattt
    10741 gatattgttg tttttcaatt agcagatggc acgatttata tcaaacgagt aatcggtctt
    10801 ccaggagaaa atgtcagcta ccaaaatgat caattgaaga tcaatgggaa agtagttaag
    10861 gaaccttatc taactaaaaa cctgaaatca gatcatgcaa atgcatccta tacgacggac
    10921 tttacactac aagagctaac gggacaatcc aagcttccgg aagataatta ttttgtactt
    10981 ggagataatc gaagagtttc aaaagatagt cgttcgtttg gcacgatcaa taaaactgat
    11041 attctaggta aagcaagatt tgtctattat ccacttgatg aaataaaatg gatccagtga
    11101 gaaacaataa agcgtgcagt ctgttgagac tgcacgcttt attgtttaat ccaaaatcag
    11161 tttactacct tagtacgtaa aatcaaacag ctcgtttaaa cacttttcta gcctctaaag
    11221 ttttgataaa tgaataaaca accattattt gaatagggat ttggatcata ttcctgatga
    11281 atctacctag aaaaattact ttgaaatctg caccaaacaa aacagacatc agccaagaat
    11341 tgataaaaag gctgactggg atcataatca ataattgggc agcgatgacg gaaggcaggt
    11401 ttatcttttt atgatataaa aataagccat taataaatcc accaaggaaa gccgtgataa
    11461 gaaaagcagc ttgaaacgtt cctgaaccaa atatcgtgaa atttaaagtg ttcgacaaca
    11521 tttcaagcaa accagcccag atcggtccat atagataacc cataagcgcc atacttaaaa
    11581 atccgaatgt gatttgaaaa taagcaggac caatcgctat cttgtataag atgacatcaa
    11641 gggctaataa aagagcagag gtcgacaaca atcttgcatt catttcgggc aatctaaaaa
    11701 cgtcttttgc aatcaaagtg ttcaaaatat tctctccttt gtcagttaat aataaaagga
    11761 taccataaaa tcaccaatgt gtcatgacac attggtgatt tacatacact ggcatctaac
    11821 aattaaaaaa agggtgagaa gactgctttt ataaactgta aaaaagagat tgtacgaata
    11881 aaaaaaatag aggcggagaa tacttatcgt atcatccgcc tttgtttttt tgttataaaa
    11941 aacgtcagat tagttatctg cttacatctt catccatacg ggttctaatg tatttccgtc
    12001 tagatcttgg acttctaatg aaaacatttc ttcttcaggc attcccatat ctacttcgtg
    12061 gaaagtacca ccattttctt ttgcaatttg agcaaatttt tttacttcat ccgcattttc
    12121 aacactaaac gaaatcaaag caccacttgt ttttttatta tcagctattt ctttatcttt
    12181 tataaatttt ttataaaaat catgagagag aagcattatc caaaaagtgt catcccatac
    12241 cattgaacca gcttcttcag tagaaaattc ttcatttttt ttaaaaccca gcttttcata
    12301 aaaagcaacg gaacgattga catcagcaac aggaaaatta acaaaaacca tactattcat
    12361 ttgtttcgtc tcctttctgt tttgttcgtc tatcatctct tatttaggaa acgctgtcaa
    12421 aaatttagat tcatatatag aaaccaaaat aaaaatttgc atcatcaaaa aagtgaagat
    12481 gtgatagaat gaggactggt tcactttcac ttaactaata gatggaattt ctttataata
    12541 aaaaagtgaa tttatattga aaaagaaaaa aacaagtaaa gccggggaat attccggtat
    12601 gaaaggaatg gagagaacaa tgaccatcca atggtttccg ggacatatgg caaaagcccg
    12661 acgagaagtt tctgaaaaaa tgaaatatgt ggacatcgta tttgaattgg tagacgcacg
    12721 attaccttta tcttctcgta atccaatgct agatcagatc attcagcaaa aacctcgcct
    12781 tgttttattg aataaagcgg atctggcaga tccacaacag acaaagctgt ggcaagaata
    12841 ttttcgtaaa caaggccatc ttgcattagc tatcacggca caagaaagta aaggaattaa
    12901 aggtctagta cctgcggcta aagaagcatt gaaagaaaaa cgggcaagag atgctgctcg
    12961 ggggatcaag cctcgcgcta ttcgggcaat ggtcttaggt attccaaatg ttgggaaatc
    13021 tacgttgatg aatcgactag taggcaagaa aattgcacag acaggaaata agcctggcgt
    13081 aacaaaaggt cagcagtggc ttcgtttagg taatgagttg gaattgttag acacaccagg
    13141 gattttatgg cccaaatttg aagatgaaga aatcggcaaa aaactagcgc tgacaggcgc
    13201 gatcaaagac cagcttgtcc atttagatga tttagcgatt tacggattag acttttttgc
    13261 ccgatattat tcaggaagaa tcagcgatcg ttatggttta tcaatcgagc aagaagaaaa
    13321 attggcaccg gaattactga tggatattac agcaaaacgt gggtataaag aagactatga
    13381 tcgtggcagc gaattagtcg tgcatgatat tcgtcaagga aaacttggac gatacacact
    13441 ggatcgttgc gaagaaatgg agcaaaaaag tgatgactga atccatccaa agtatcaaag
    13501 aacgattgaa aagcatacag agaatagatg atcctacact agaatcatta agaaatgatc
    13561 cacgcaaagg cgtgcagcaa gcactggctt cttttgaacg acgattgaaa aaggaagcat
    13621 taattaagga aaaatatgaa gaaatgtttt gctttgaaaa gcaagggtac gcacaaggtc
    13681 accaatggat tgccggcatt gatgaagtag gaagaggacc attagctgga cctgtcgtgg
    13741 cagctgcagt tatcttgccg aaaaatgaga tcatattagg gctgaatgac tccaaacaat
    13801 tatcagagaa aaagcgagaa cttctttatc aacaagttca agaaaaagcc ctggcaatcg
    13861 ggatcgggat cgttgatcag cagacaatcg atgatgtgaa tatttatgaa gcaagtaaat
    13921 tagcgatgat ccaagcagta gaaaaattgt ccattgtacc aagctatttg ttgatcgatg
    13981 cgatgacctt ggatttgcct attcagcagg aaaaaataat caaaggggat gcacgatctg
    14041 tgtcgattgc tgctgcaagt atcattgcta aagtatatcg agaccaattg atgaaagaat
    14101 atgacgtgct ttatcctgga tatggtttct cgcataatgc aggttatgga acaaaggaac
    14161 acttagaagg acttgaacga caaggaatta cgccaatcca tcgtaaaagt tttgcacctg
    14221 taaaaaaata tttacaataa aaaacagccg cttttacgta tcttattaga aagagagacg
    14281 aaaggcggtt ttatgatgta tcaaatagaa gaaaatttat tgaaacttgc ttatacaaaa
    14341 ggaatcagta gtcaaggaaa atgggcactt gctaactgga tgatgcagta tccttataaa
    14401 gaaatcggat ttgaagacat cattaagata ggtaaaataa ccaaacaccg ggagctgttt
    14461 cgcaaaagtt ggcaggaaat tcacgctaac tggggcaaaa tccaatcgaa acagtcgttt
    14521 atcacttgct ttgatcccaa atatccccct cagctgcttc acttgaccta tccgccaatt
    14581 gtactgtttt ataatggtga tctatcttta ctttcttgta acatgttaag cgttgttggt
    14641 ggaagacaca cttctgcttt agccaaaaaa acagtttatt atttactaaa accagtgata
    14701 gaagctggat atgtgatcgt cagtggctgc gcgaaaggga tagatacata cggtcaccaa
    14761 gcagcaatct ctcatggtgg acggacaatc gcagtcattg gaacagggat agagcaagtc
    14821 tatcctgctg aaaatcgtga cttgcaagag gtgattgcaa atgatcatct tcttttgagt
    14881 gaatatcctc ctcaatccgg cccgaaaaag cattgttttc caatgagaaa tcggattatc
    14941 gcagctttgt ctaaaggaac atgtgtcttt gaagccaaga aaaaaagcgg ctctctgata
    15001 acagcacagc aagcattgga tcttggaaga acagtttttt ctgttccagg aaatatttta
    15061 acagaacatt ctaccggatg tcatcatttg atccaagacg gtgcagtttg cacggtttcg
    15121 ggtgaagata tcttggcaga atttaaagaa taacaccata cttttcattt gcttttcttg
    15181 acaaacgttt tctgaatgga tatgattagc tacgatttgt atgaatcctc ttttaatagt
    15241 aggaacacga caatggaaag gagtccattc agtaatggcg tataaatatt tagtgattgt
    15301 agaatcacct gcaaaagcca agacaattga gaaatatctt ggacgaaact ataaagtaat
    15361 ggccagtgtt ggccatataa gagatttacc gaaaagtaaa atgggtatcg attttgaaaa
    15421 caattatgaa ccccattata tttctatacg cggaaaaggc gatgtcatca aaagcctgaa
    15481 agccgcagca aaaaaagctc aaaaagttta cttggcaagt gacccggata gagaaggaga
    15541 agcgattgct tggcatttag cgtacctact tgggttggat ctgaaagaaa aaaatcgggt
    15601 ggtcttcaat gaaatcacaa aagacgcagt caaagcagct tttaaggaac caagaacgat
    15661 cgatgtagat ttagtagatg cacagcaagc tcgtcgtacc ttagacagaa tcgttggtta
    15721 ttcgatcagt cctattctct ggcgtaaggt caagaaaggg ttaagtgcag gacgtgtcca
    15781 atctgtcgct ttaaaaatta ttattgaccg tgaaaaagag atccgagaat ttgttccaga
    15841 agaatattgg agcatcgacg gtaattttaa aaaagctcgc aagaaattca aagcaaattt
    15901 ctggggaatc gacggtaaga aaaagaaatt accagatgca caaagtgtaa aagaagtcac
    15961 tgctagaatt gatggaacag acttcgaagt taaaaaagta gaaaagaaag agcgcaaaag
    16021 aaaccctgcg aatcctttca ctacaagtag cctgcaacaa gaagctgccc gtaagttaaa
    16081 ctttcgaaca agaaaaacca tgatggtagc acagcaatta tatgaaggaa tcactttggg
    16141 taaacaagga acagtcggct tgattacata catgcgtact gattctaccc ggattgccga
    16201 tacagcaaaa gcagaggccg cagcatttat tgaagatacg tatggaaaag aattttctag
    16261 acatgatcac cgaaagatca aaaatgctca aggcgcacaa gatgcccatg aagctgtgcg
    16321 tccaacgagt gtgcttcgta caccagatga actcaaacaa tatttagata aagatcaatt
    16381 gaaactttat acattgatct ggtcacgttt tgtggctagt cagatgacac cagctatttt
    16441 agatacaatg aaagtgacat tagaacaaaa tggtgtcatc ttcattgcga acggttcaaa
    16501 aatcaaattc aaaggattca tgcaagtgta tgtggaaggt agagatgacg gcaaagaaga
    16561 gaaagaaaat attcttccag aacttgaaga aggcgatgta gtccaatctg ttgatattga
    16621 accaaaacaa cattttactc aaccaccagc acgttttagt gaggctacgt tgattcgtgc
    16681 actagaagaa aacggggtag ggcgaccttc cacttatgca ccaacacttg atacgatcca
    16741 aagacgctac tatgtaaaat tgacacaaaa aagatttgag ccgactgaac taggagaaat
    16801 tgtcaattca ttaatctgtg aattcttccc tcaaatcgtc gatatccatt tcacagcaga
    16861 aatggaagga gatttggata aaattgaaga aggcacggaa gcgtgggtca aagtggttga
    16921 tcgtttctat aaaccgtttg aaaaagaact gacgaatgcg gaagaaaaaa tcgaaaaaat
    16981 tcaaatcaaa gatgaaccag caggattcga ttgtgatgtc tgtggacatc cgatggtgat
    17041 aaaattaggg aaatatggga agttttatgc ttgcagtaat tttcctgatt gtcggaatac
    17101 gaaaccaatc gtaaaagaaa tcggtgtgac ttgcccagta tgtcatgaag gacaagtcat
    17161 tgaacgaaaa tctaagaaaa acagactatt ctatggttgt tcccgtttcc cagaatgtga
    17221 attcacttct tgggataaac cagtgggcag aaattgtcca aaatgtgacg gatatctggt
    17281 cgagaagaaa gtcaaaggcg gtaaacaagt agtctgtgta aatggagatt acgaagaaga
    17341 catccaaaaa taatagaaaa gaggttcttt cattgaaaat ggtaaatgtt gttggagcag
    17401 ggcttgccgg aagtgaagct gcttggcaga tcgcacaagc aggagtccct gtccgtttat
    17461 atgaaatgcg tccagtcaaa tcgactgagg cgcatcacac aagtaatttt gctgaactag
    17521 tatgttccaa ttcattaaga ggaaacagtt tggctaatgc agtagggtta ttgaaagaag
    17581 aaatgcgccg attgaattcg gtagtaatca cttcggcaga tgaaacagca gtgccagctg
    17641 gtggtgcctt agctgttgac cgcgattctt tttctgaaac aattacccga aaagtcaaag
    17701 agcatccatt agtaacagtg ataaacgaag aaattactga aataccggaa ggaatcaccg
    17761 tagtagcaac aggacctttg acttctcatc cattagcaga aagcatcaaa gcattcaatg
    17821 gttcagatgg tttttatttc tatgatgcag ctgcaccaat cgtagacaaa gctacgatcg
    17881 atatggataa agtatatttg aagtcccgct atgacaaagg agaagcagct tacttgaact
    17941 gtccgatgac agaagaggaa ttcaaagctt tccatgaggc actaatcaat gccgaagtgg
    18001 cagaacttaa atcttttgag aaagaaaaat attttgaagg atgtatgcca attgaagtaa
    18061 tggcacagcg aggattcaaa acgatgcttt tcggacctat gaaaccagtt ggattggaag
    18121 atcctaaaac tggcaaacgt ccttatgctg ttatccagct gcgtcaagac aatgctgcag
    18181 cttctttata caacatcgtg ggctttcaga ctcacttgaa atggggcgag caaaaacgtg
    18241 ttttccggat gattcctggg ttagaaaatg ctgaattcgt tcgctatggt gtgatgcacc
    18301 gaaacagctt tatgaattcg ccagaattgc tgaaaccgac ttaccaatca acgaaacgcg
    18361 aagatctgtt ctttgcaggt cagatgactg gtgtagaagg ctatgttgaa agtgcagcga
    18421 gcggactact tgctggaatc aacgccgcac gcttagcaaa agaaatggaa ccagtcgagt
    18481 ttccacaaga aacagctatt ggaagtatgg cttactacat tactcatgcg gaaggaaaac
    18541 atttccaacc gatgaatgcc aactatggtc tgtttcctga actgccagag cgcattcgag
    18601 acaaaaaagc aagatatgaa gcaattgcac aacgagcttt aaaagctaat gaggcaataa
    18661 aaacagaatt agaaaaaact gcaatcccaa atgagtaagg aaactccatg aagagggtga
    18721 gacggatagt ctcgctcttt ttcttgcgtt tttcttaaga atttctcaaa caaaatgtga
    18781 ggaattgcgc caattgtgaa caaacttaga aatattggtt aaattctgac aatttatttg
    18841 tctttttata aacgctgtgc taaattattc tctgggacaa aaggaaggtg agaaaatgac
    18901 agaagccacg tgggaacaaa aatttttgcg ttatttgatt gtcgaaagag gatattcaga
    18961 aaaaacgaaa gaagcatatg aagaagacat tctccatttc aaacattttt tggaggaatc
    19021 tggagacgct gatctgctga aggcggaata ttttacggtt cgtgtctatt tgagtgcatt
    19081 agctgatcat cgtcctagct atagcagaaa ttctatcagt cgcaagatag ccagcctacg
    19141 ttcgttttat cagtatcttc tcaaagaaga agtgataaaa gaaaatccat tttcttacgt
    19201 tcatctcaaa aagaaaaatt tgagactgcc acgttttttt tatgaaaatg aaatgcaagc
    19261 actttttgac agcgtagcag gagatagtcc tttggatttg aggaaccgcg ctttactgga
    19321 agtcctttac gggtcaggca tccgtttaag tgagtgtagc agtttgtcgg tatcagacat
    19381 tgattttgat tcagaggtca tgttgatcca tggtaaaggg aacaaagaga gatatgctcc
    19441 tctaggttct tttgctcaag atgctctgca agaatatttc agggaaggcc gccaggtatt
    19501 gatgacaaaa tatcacgaag agcatgatat cgtttttgtg aatcatcatg gaaaaccaat
    19561 cacgcctact ggaattgaat atgtcttgaa tcaaatgatc aaaaaaagca gtttggatag
    19621 tgatatccat cctcacatgc tgcgccatac atttgctact catttattaa ataatggggc
    19681 agatttgcgg acagtccaag aattgctagg acatgccaat ttatcgacta cccagatcta
    19741 tgcccatgta acaaaagaaa gtttacaaaa aaactatcgt tctttccatc caagagcgta
    19801 aaaataagga aaagaacaaa tttaggagga aacaaacatg gtcgaatcac catttcattc
    19861 aaccactatc tgtgccgtcg aaaaagacgg gaaatttgct atggctggag atggccaagt
    19921 aacaatgggt gaatctgttg tcatgaaagg cactgcaaag aaagtacgac gtatctacaa
    19981 tggagaagtt gttgtaggtt tcgccggaag tgttgcagat gcgtttactt tagaagaaaa
    20041 atttgaaggc aaactaaacg aatataacgg gaatctacaa cgcgcagctg tcgaattagc
    20101 tcaagaatgg cggacacagc aatctatgca aaaacttgaa gcgatgctga tcgtaatgaa
    20161 tgataaagaa atgcttcttg tttcaggtac aggagaagtt atcacaccag atgatggtat
    20221 cttagctatc ggatcaggag ggaattttgc gttggctgca gcccgagcaa tgaaaaacta
    20281 tgctcaaaac gacatgagtg cgaaagaaat tgcagaaaat gcgttaaata ttgccgcaga
    20341 tatctgtatc tttactaatc ataatattat cgtagaagaa ctgtaagagg tgagtagata
    20401 tgtatgaatt aaaacaaaca ccaagacaaa tcgtaagtga attagatgaa tatatcgtag
    20461 gacagcacgc agcaaaaaaa tctgttgccg ttgctttgcg taaccgctat cgccgtttgc
    20521 agttagatga aaaaatgcaa caagatatta caccaaaaaa tatgctgatg atcggaccta
    20581 caggggtcgg taaaacagaa atcgcacgac gtttggcaaa aatcgtcaat gcaccatttg
    20641 tcaaagtcga agcaacaaaa tttactgaag tcggttatgt cggaagagat gtagaatcaa
    20701 tggttcggga tttagtagaa aatgcgatcc aaatcgtaga aaaagagcag tacagccgtg
    20761 tatacacaag tgctaaaaaa aatgcagaaa aacgtttggt aaaaatcttg gtacctggta
    20821 tcaaaaaaga acagaaacaa tccactaatc aatttgaaca aatgatgaat ctttttaatg
    20881 ctagtcagca aaataccgct gaaccgcaag aagaagtgac agaagatatt cgaatcaatc
    20941 gccgtacagt cttggaacaa ctacaaaagg gtttactgga tagtcgtgaa gtaaccattg
    21001 aagttgaaga ccaaaagaaa cctgcaccaa tgatgaacaa tggattggag caaatgggga
    21061 tcgacctaaa tgaaactcta ggtgctttga caccgaagaa aaaagtggaa cggacggtaa
    21121 cagtcaaaga agctttagag ttattgatca aggaagaatc agcaaaatta gtcaaggatg
    21181 ctgatatcca tagtgaggca atcaaattag cggaagaaag tggaattatt tttatcgatg
    21241 aaatcgataa gatcacttct aaatcacagc aatctggaga agtatccaga gaaggcgtcc
    21301 aaagagacat attgccaatt gtcgaaggtt cacaagtaaa cacaaaatac gggatcatcc
    21361 aaacagatca cgtcctattc attgcttcag gagctttcca tttgtctaaa ccaagtgatc
    21421 taattccaga acttcaagga cgcttcccga tacgggtaga attagatgat ttgacagctg
    21481 aagattttgt gaagattctg accgaaccaa ataatgcttt agtaaaacaa tacgtcgcgt
    21541 tgatcggtac agaaaatgtg tcagttacgt ttacaaaaga agcaattgaa cggatcgctc
    21601 aaatcgcttt cgatgtcaac cgtgacacag ataatatcgg cgcacgcaga ctgcatacca
    21661 ttttggaaaa actactagaa gatctattat ttgaagcacc agatatgcaa atgggagaaa
    21721 taacgatcac agaagcatat gtggatgaca aacttggctc aattgcggca aatgaagatt
    21781 taagtcgcta tattttataa aaaatcggag gacgacaatg acagacttgc taacgaaaac
    21841 ccgcaagata aacacacttt tgcagcaaaa gaatactttt gacatgcaag cagatttgcc
    21901 ttacgataaa atggctgtca cactaggtgg tattcttgac agcaacacct atatcatagg
    21961 aagtgacgga gtattactag gttataatga acgtcacgat gtgaacaatg aacgaatcaa
    22021 aacaatgttt gttgagaaac aatttcctga atcttatact gatacagtgg cacagttgat
    22081 gaaaactgaa gctaatatcc caattactag tgatattact gcatttccgg tagagtcaag
    22141 agagaaatat ccatttggtc tgacgactgt cgttccgatc tttggagctg gcgaacgttt
    22201 gggtacgatt atcttatctc gtatggaaca atcgtttgca gatgacgatc tagtcttagc
    22261 agaatatgga gctacggttg tcggtatgca gatcctttat cagaagtctc gaagcatcga
    22321 agcagatgta cggagtgcaa cggctgtcca aatggcaatc aacacattat cttatagcga
    22381 attgaaggct gtacaagcta tctttgaagc attggatgga gatgaaggtc gtctgactgc
    22441 ttctaatatt gccgatagta tcggcatcac tcgttctgtg atcgtcaatg ctttacgaaa
    22501 attggaatca gctggaatca ttgaatctcg ttcgttaggc atgaagggaa cttatttaaa
    22561 agtattgaat tcccgtttta aagacgagtt agcaaaacac agttattaga agtaagggga
    22621 ctggggcaga tagaaatgtt tgttccagcc ttcccatttt aaaaagtaag tcgaagttgg
    22681 aggaatggca atgactgtta ttattcaaaa tgaccagctg atcgccgaaa tcgctgaaca
    22741 cggtgcggaa ttgatcagtc tgaaatcaaa agaacatggt ttagagtata tctggcaagg
    22801 tgatccagcg tattggggca gacatgcacc ggttttattc ccttttgttg gacgtttaaa
    22861 agatgatcag tatgtttatc aagggaaaac atatcaaatg gggcaacacg gatttgctcg
    22921 ggatatggat tttgaagtca ttgaacatgg acaagaaatg gctagtttca tcttaaggag
    22981 tacgccggag acattagaaa agtatccatt tgattttgaa ttagtgatca gctatgaatt
    23041 aggaggagac ggcatcacgg ttcattatca agtggcaaat accggtgaaa aagaaatgtt
    23101 cttttctatc ggtggacatc ctgctttcaa tgtacctctt gaagaaggat tgtcatttga
    23161 agatttctat ctggcatttt ctccattgaa atcaagatta cgcctaccat tgaaaggccc
    23221 attcatcgat atggatcaaa agacgttagg tcagacaaat acgaatctgg ctttgaatcg
    23281 tgaacttttt gagcaagatg ccttggtttt tgaaacaagg ggtctaaatg cgtacgccat
    23341 tcgaagtgaa aaatcttccc atagcgtaac tttaagctat aaagatatgc cttatgtagg
    23401 gatctggtct ccttatccaa aagaagcgcc tttcgtttgt atcgaaccgt ggtgcggaat
    23461 cgctgacacg gtgaacagta gtggaaactt gatggaaaaa gtagggatca atcaactagg
    23521 tgcccatgaa atcttcaaga caaaatattc catcacagtg aagtaagagg ttgtgaccga
    23581 gaagttagca gggaacaccg tttaccaagg tgtgtaaaaa agctatcctg taagtaataa
    23641 gggagtgtga catcgattt
//
LOCUS       DAFEPQ010000039        22154 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.039, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000039
VERSION     DAFEPQ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.039"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            349..2391
                     /locus_tag="LP543_001661"
     CDS             349..2391
                     /locus_tag="LP543_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2321829.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            2402..3022
                     /locus_tag="LP543_001662"
     CDS             2402..3022
                     /locus_tag="LP543_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBL2321830.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            3034..3492
                     /locus_tag="LP543_001663"
     CDS             3034..3492
                     /locus_tag="LP543_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2321831.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            3511..3789
                     /locus_tag="LP543_001664"
     CDS             3511..3789
                     /locus_tag="LP543_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2321832.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            3814..5181
                     /locus_tag="LP543_001665"
     CDS             3814..5181
                     /locus_tag="LP543_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBL2321833.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            5196..6341
                     /gene="dgoD"
                     /locus_tag="LP543_001666"
     CDS             5196..6341
                     /gene="dgoD"
                     /locus_tag="LP543_001666"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HBL2321834.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            6368..6859
                     /locus_tag="LP543_001667"
     CDS             6368..6859
                     /locus_tag="LP543_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321835.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            7302..8501
                     /locus_tag="LP543_001668"
     CDS             7302..8501
                     /locus_tag="LP543_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2321836.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(8996..9754)
                     /locus_tag="LP543_001669"
     CDS             complement(8996..9754)
                     /locus_tag="LP543_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL2321837.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            9922..10857
                     /gene="pfkB"
                     /locus_tag="LP543_001670"
     CDS             9922..10857
                     /gene="pfkB"
                     /locus_tag="LP543_001670"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBL2321838.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            10854..12299
                     /locus_tag="LP543_001671"
     CDS             10854..12299
                     /locus_tag="LP543_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2321839.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            12327..12782
                     /locus_tag="LP543_001672"
     CDS             12327..12782
                     /locus_tag="LP543_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2321840.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            12801..13775
                     /gene="lacD"
                     /locus_tag="LP543_001673"
     CDS             12801..13775
                     /gene="lacD"
                     /locus_tag="LP543_001673"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBL2321841.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(13886..14447)
                     /locus_tag="LP543_001674"
                     /pseudo
     CDS             complement(13886..14447)
                     /locus_tag="LP543_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            14591..15205
                     /locus_tag="LP543_001675"
     CDS             14591..15205
                     /locus_tag="LP543_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2321842.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            15446..15829
                     /locus_tag="LP543_001676"
     CDS             15446..15829
                     /locus_tag="LP543_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2321843.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            16192..16701
                     /locus_tag="LP543_001677"
     CDS             16192..16701
                     /locus_tag="LP543_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBL2321844.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            16801..17451
                     /locus_tag="LP543_001678"
     CDS             16801..17451
                     /locus_tag="LP543_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321845.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            17902..18840
                     /locus_tag="LP543_001679"
     CDS             17902..18840
                     /locus_tag="LP543_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBL2321846.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            18853..19905
                     /locus_tag="LP543_001680"
     CDS             18853..19905
                     /locus_tag="LP543_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2321847.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            20171..20806
                     /locus_tag="LP543_001681"
     CDS             20171..20806
                     /locus_tag="LP543_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBL2321848.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            20997..21788
                     /gene="msrA"
                     /locus_tag="LP543_001682"
     CDS             20997..21788
                     /gene="msrA"
                     /locus_tag="LP543_001682"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL2321849.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT         7055 a         3509 c         4661 g         6929 t
ORIGIN      
        1 acacaaaatt attgacgctc ccaaaagcat tgtttttagc agtttaatat tattcttttt
       61 caattagact gacttgattt agttacggaa tagatacttt aatgtgttga agaagcaacg
      121 tcccgtgttt atttactaaa gcatccaata ttctaccgaa tatttaatat tcacattagc
      181 cctaaaataa ttgtcatacc atgttacatt tgatataaca gcgttcattt taaaaatagc
      241 tgttagtgtg acaaaacatt aaaccaagtg tgacaaaaaa cgtgatgaaa gctttccgga
      301 aacgtatgtg aacaagtata taatgatttt gtcaggagga tagtcaaaat gaataaacga
      361 attcgtgaaa tactcaaact gattattaaa aaccctgaaa tgaaactttc cactttaaca
      421 agtgaacttg atttgacacg tcgtcaaatc aattacgcga tcaatcaatt taatgaagaa
      481 ttgaaaacaa agaacattcc gttaattcag agaagtcatt ctggaaatat tacggtacca
      541 atcgaggtta tccaaatgat atcacagcct gattacgaca taggtgatga tcaggaactt
      601 gtcgttctca ctgatagtga aagacaagca ttgatagtga tgactctgat cgctaatatc
      661 gattacatca gtctggatca tttggtagat gcggttgatg ttagtaagac gactgttatg
      721 gaagatatca aacgaacgga accgttcatc agaaattata gtctgacaat tcgatataat
      781 cggatgaatg gttaccaatt ggttggttcg gaacaccgta tcttacagct gttatcggat
      841 ctagtaaaac ggtttccgat ttttcgccga gaaatgattc gcagaaaatt ggcacaagat
      901 gtttctgaag aagaaatcgt ccatttgatc cataacatgg aacaaatgtt gcatcttagt
      961 tattctgatg agtcggtaga ctatttgcag acatcagcga gatttttagt aagccgtggg
     1021 atcaagatgt cagataagca cgcgtttttt cccgatgatg tgacggatac tcctgaattc
     1081 cgaatgctga tgatcttggt acatgaaaca ggttggagtc tatccaaaag ttatatggag
     1141 tggttcacgt tattattttt aacttccaat atttttgaga gcaaaactac tcaaaattat
     1201 gattcggatc aagaattgcg aaaattgatc agccaaatgg ttgaaaattt ccaaaatcaa
     1261 acgttgattt tgatcgatga ccgagaagac tttgaacgta ggatactgag tcatctgcgt
     1321 ccagcctgtt ttcgaatacg ctataacttg agtttaggag tttattcaat agatagtctg
     1381 atcaaggata gtaatcatgc gattttgatt gatttgatga aagaattgat tattcctatt
     1441 gagaattggc taaataaggc ctttccaaat gatgaattag atcttttatc ctattacttt
     1501 ggctatcaat taagtaatca taataaattg aataagcaac gacaaagggc tgtagttgtt
     1561 tgtactaatg gtgtcatggt ctcaaagtta gtacgagcca atatggaaaa actttttcca
     1621 gagatccatt ttttagcttc cctatctgtg cgtgatttct acaaatttga agctgattat
     1681 gatctggtct ttactacgac gccactaaaa agttctatgc tgcagtttat gatcgagcca
     1741 atcatgacat atcagcaaca aatcagttta cgatatcgag ttttgagtga tttaggaatt
     1801 actaaggttg atcgagcaat ggatgaattg ttaacaatca ttaggaaata tgcaaatatc
     1861 accaatccag tttcgttgcg agaagaactg cagtactttt tgatcaagga agatggaagc
     1921 acgccgctgg acaacttcaa tgtattgcct tcattgactt attacttgcg gccgagttat
     1981 gtacaaatcg ttgaagaaga gatgtcttgg gaacgagcag tggaggtggc ttgccgaccg
     2041 ttattggaac atcaaatcat tgactcgcgt tttatcgctg attgcttgga tcaaatcaga
     2101 caaccgggat atgcaggtta cttgggaatg aagacttgca ttccccatac aacggtcgat
     2161 aaaggtgtaa tcagtgacgg tgtcagcatt ttagtttgta aaaagccgat acgatttcct
     2221 aatggtgaag atgtcagctt gattttgccg ctttcatttt ttgatttaac gaagcatctg
     2281 cgagccatca atcaaatcgc tgatatcgcc aaagatgatt cattgattga gcagttattg
     2341 attgctgatg acgaaaagac agtttatcaa ctattaagac aaagatctta aggaggaaaa
     2401 catgagacta gcagattatc ctaaattgac agtgatcatg cgtggttata cttttgatca
     2461 ggcagatgct attttacaag caatgcagga atttgaccat caatttgccg tagagatgac
     2521 aatgaacaca aaaggagctc ttgaaaatat acatgaacta aatcaacgtt atggtgacac
     2581 gacttatatc ggcgctggga cggtaaggac attagaacaa gcccaagcct gctatgaggc
     2641 tggtgcaaaa tttttattag gaccacatat gttcacaaaa gaaatgttgt cttacgccaa
     2701 taagaaagga ttattggcgg tacctgccgc aatgactcct tcagaagtcg accgtatgtt
     2761 taaagaagga gctgatattg taaaagtatt tcctgcagcg gttgttactc cacgtttttt
     2821 caaggatatt caagcacctt taggtaagtt accactaatg ggggttggtg gaatcagtaa
     2881 ggagaatgcg aaagagtttt ttgaacaagg tgctagctat ttaggtcttg gttccggtat
     2941 gttcaacaag caagacatcg aagagctaaa tgttaaaaac ttagcacgtt cgatgaaaga
     3001 attaattgag gctattaaat aggagtgagg actttggaac tttatttgaa tgaggaactc
     3061 gtttttcgaa atgttcaagc aaaaacagat agtgaagtct tagcgttttt agcaagcgag
     3121 atgtacaaaa aaaactacgt gaaggaggaa tatattcaag caatccaaga acgggaaaaa
     3181 gaatacccta ctggattacc atcaacacca ccaggaatcg caattcccca tgcaaactat
     3241 gaaatggtca ataaaacaac tttagcaatt gcgactctta aagatccggt cctatttcat
     3301 aatatggaag ataacaatga gcagttacca atccaaattg tgatcatgat ggcgattggg
     3361 gaaccacatg ggcaagttga aatgttacaa aaaattgttg gaatcataca agatgagcca
     3421 ctgcggcaag aaatgatcag agcaggcaat gatactgaat tattagaact attaaagaaa
     3481 gcggtttttt aatataagga ggaaatcata atgaaacgaa ttttagtagc atgcggaaat
     3541 ggtatcgcca cttcaacagt agtagcaaca aaagttagag agtatttgca acaacatggc
     3601 attgatgttt caacgactca aacaaaattg atggaagttc caggaaaagt tcaagactat
     3661 gatctattgg tgacgactgg tcaatttgat ggtcaaacag gtggtgtacc agtaatcaaa
     3721 gggatgccga ttttaacagg tatcggtgcc gatcagacaa tggaagagat tttgaactta
     3781 ctgaaataat agctgatcaa aggagctgaa aaaatgaaaa tcattatgaa cgtctttaac
     3841 ttctttattg atgctggacc aacagttatg ttgccagtca tcatcacgat tattggtttg
     3901 atttttgggt taaagatttc tcgtgctttt aaatcaggtt tgacattagg tatcggtttt
     3961 gccgggatca agttgatatt ggactttatg acgaccaatg ttggaccagc tgccaaggcg
     4021 atggttgatc gaactggtgt taaactagat gctttggatg ttggctgggg ttcgattgcg
     4081 gctgttactt gggcatcgcc aatcattcca atcttgattt ttgccattct attggtcaat
     4141 atcgttttgc tgattttgaa acgtacacat acgctggatg ttgatatctg gaactatcac
     4201 catatggcaa tcgttggtgt tatagtgtat tttgtgacta agaatgtctt cttaggtgta
     4261 ggtgcatcag tagtgatggc aattgcgact tttaagattt cagactggtc gcaaccgatg
     4321 gtagaaagtt tcttcggaat ccctggtgtt tctttaccaa ctgtttctgc gctttcatca
     4381 ctggttattg cttggccgtt aaattggctg ctggatcgta tcccgttatt cagaaaatca
     4441 aaattcacca tcaaagatgc acaaaaatat ttgggatttt ttggtgattc tatgatcatg
     4501 gggttagtta ttgggatggt gattggtgtt ttagccggct atgatatcaa agcggtttta
     4561 caattaggtg tcagcatgtc agctgttttg gttttgattc ctaagatgac ttcattattt
     4621 atggaaggat tgatgccaat ttcagatgct gcacaaaaat ggtcacaaaa aaaattcaaa
     4681 ggtacacgtt tatttatcgg attggatgct gcagttgttg taggtaatcc agatgttatc
     4741 acaacagcat tgatcattat tccactaacg attgcattag ctttagtatt gccaggaaac
     4801 cgtgtgttac cattcgcaga tttagcggtt gttcctttcc gcgtggcaat ggtcgttgcc
     4861 ttgactcgtg gtaatttatt aaaaaatatc attattggct tagttgtgac agcttcattg
     4921 ctatggtgtg ggtcagctac tgcgccaatg ctgacagcaa ttgccaaatc ggttggtatt
     4981 aacttaggaa cttcttcaat gttaatctca tcatttgctg cgacttccat gatccaaagt
     5041 tatttggtat ttatcgcatt tgcttacaaa ccaatcatcg gtattccagt gttgttatta
     5101 gcctttgcgg gcgcatggta ttacttcgat ggtatcaaaa aagttaatca tgaaatggat
     5161 gaaccattga aggcacaata gtaggagggt ttaaattgaa aatttcagat gtcacagtgt
     5221 ataaagtaaa accaagatgg atttttgtta agatctcgac tgacgaagga atcgaaggct
     5281 ggggagaaat gatctcagga actaaaacag aaacggttgt tgctggagct tatgagatag
     5341 gaaaccggct gattggacgt aatccgtttg aaatcgagcg cctcttccaa gaaatgcatc
     5401 gatctttctt ccgaggcggt cctattaatg gaaccatcgt ttcgggttta gaaatggctc
     5461 tgtgggatat caaaggaaaa gcgtttaatg ttccggttta tgaattattg ggcggtgcag
     5521 cacgtgataa aatcaaggtt tattcttgga ttggtggaga tcgaccaagt gaagttgcag
     5581 aacaagcaca agatcggttt gatcggggct ttacggccgt caagatgaac gcgacagaag
     5641 agctgcatta tatcgattcc taccaaaaag ttgatgaagt ggtggaacga gttgcgtcta
     5701 ttcgcgaacg ttttggtgat gcaatggata ttggtgttga ttttcatgga cgggtccata
     5761 aaccgatggc aaaagtgcta gcaaaagctt tggagccatt ccatccaatg tttttggaag
     5821 aagtcgtttt acctgaaaat gaagaacact ttaaagaagt agctgattct gtggctgtac
     5881 cattagctac tggtgaacgc ttgtacactc gttggcaatt caagaacctc tttaaacaag
     5941 gtactgttga tattatccaa ccagatgtgg ctctttgtgg gggaatcttg gaaacccgta
     6001 aaatcgcagc gatggcggaa gcttacgaca tggctgttgc gcctcatgcg ccatatggtc
     6061 ctgttgcact agcagcaact ctgcaagtag actcatgtac gccaaacgtc tttatccaag
     6121 aacaaagctt agggatccac tataacaaag gttttgatct gttagatttt gtgaagaaca
     6181 aagaagtttt ccaatataaa gacggctacg tagatctacc ttcaaaacca ggtctgggct
     6241 tggaaatgga tgaagacaaa atcaaagaaa ttgcacaaga aggtttgatt tggaccaatc
     6301 cgcaatggaa aaattatgac ggtacgatcg ctgagtggtg ataaatgaaa ataaaggatg
     6361 atgaaagatg gcagaagcac ttatgatcac agttttttta ggactgctga ttattttgca
     6421 aataatcgat caaaagaaaa ttatcgtgtt tgctcagagt agtcctgtaa ggaagagttt
     6481 gagtatttta ctgacagtcg ccatcctgtt gattttctgg ccggaacatc tgactgatca
     6541 gattaaactg gttgcttttg cttctgtcat tttgatcaat ggttttttga aggagggtct
     6601 agcaaaagat tctgttgtta gatttggagt attgaacggt aactttaatc gatatcctca
     6661 agtccaagta gaaaaactag gtgacgataa atcatttatt tcattttaca aaagcaagaa
     6721 taattctttt tcaatgattt ttgataaaga tcaaactgaa atgatggatg tctttcaaga
     6781 acttgatatc cattccaaaa ttactttaga tcaaagttcg gaagaaaaaa gagcagttgt
     6841 aaaaaaagat aatgaatgat tctactaccg aaattatgat ttctttatca cgaataatga
     6901 aacttgtttt aatgaatcat attgattgaa gtatacaatg tatattaagt tttggaaaga
     6961 aatatattgg tacaagatat agcaggaatc gattactcga tccctgcttt ttttaacttc
     7021 ctttgcagca aaaaaagctc gtaatctaag agatgatgga tagtttttag aatatatata
     7081 ttttcttcaa ttccagaaaa aaatgaccaa gctagtatgc taaaaaagta cgatgcttct
     7141 atgatctttt ttccgtcttt attaacagat gttttaattg atcaacgcat attgtaatct
     7201 accctaattt ccatagttct tcaccacttt taaaaatagt aaaaccccta caagtatgct
     7261 atagtaaatt gctaaagaaa aaccaataag gtgggagatg tgtgcgcgat caaacaagcg
     7321 gaaaaatgat tcgtttttat tatacaagtt tgcattttct ttattggatg atgttttgtt
     7381 ctgtctatgg gtttgcgaca ttgtttttga tttatttcaa gattgatcca gctaaaatcg
     7441 gagggatctt agcattggtc aatattgttt caacggtgct tcagcctttt gtcagtcaag
     7501 tgatcatcaa gaaaagaaag atcccactga tcaccgtgct gaagacgatg atagcaggat
     7561 taggagtgat tctcttagct ggggtattga ttccgaattt gccgatcatt acttatatct
     7621 tgggtgggat tttagtagtt tctatgcaat catttctcaa tgcgctggca tttgagtatt
     7681 caaataccgg ctacaggatc aactttggct tttcaagagc tggtggttca ttttcttatg
     7741 ctgtaacttc ttttttactg ggacagctgt tagcgattta ttctgccgcg attttgccaa
     7801 tcttggttat agttgaagca gtgatatttt tcgttatttt gcatctgctg ccggatgtaa
     7861 aaaatcaaga agtttttcct atgacaaaag aacaggatcg ctcagtttct ttgagaaaaa
     7921 aatatccttt cctgattttg ttatttttag gtttcagttt tctttttact ttccacacca
     7981 tgatcaacag ctttttggca cagatctttg aaaatatcca cggcggcagt aaagaagttg
     8041 gcatcgcatt gatgatcgcc gctttatgcg aattaccggg aatgatcttt tttgaacagc
     8101 ttgtcaaaaa gaaatccagc aaattttggc tgtctgttgc cagtatttca tttgctgtca
     8161 gaggattcat gatgctgctg gccagttcga tcctgatgat ggaaatgacg catctcctac
     8221 aagccttcag tttcgctttg tttattccag cttcggctta tttgttcaat caatttctgg
     8281 cggatgatga tcgtgtattt ggacaaacga tgatcatcat cgccacaact cttggtgggg
     8341 tgataggaaa tgtcttgggc ggcagtctgc tgcagcattt cggtgtatgg gagatgctga
     8401 ttttcggtac tggttttgcg gtgctaggtg ctttattaac tattttgggc attcgaaaaa
     8461 taccgaagac aggaaacgat agtgattctc ttgttgagta gcataaaaat acttagatac
     8521 tagttttata taaataacat atagtgatta ttggcgtaat tacaaaaaac gagaataact
     8581 taagtaagca tttctaaata attgagaaac ctcatctaaa aagataagaa aatatcccta
     8641 ataaaacttc tgtaacattt tagataactg attgtaatct gaataataaa ctataaggag
     8701 acggttagac ggtctccttt ttttgcgtat tataattcta gaattgttgg aaattgataa
     8761 taattaacaa gaaatattag acaggaactt cgaactgctt atcacattct tattattata
     8821 tatttgcgta agtagtcctg gtagtctcaa tgcactattc tatcttgaaa ctaagaagtt
     8881 atagaaggag caatgataga aagcacgaga atacaaatag cgattaaata tttttctaga
     8941 caaccttagt taattcacac agatatattt taggtacagt cttacagata tagagttagt
     9001 gctgtgtgaa tcctgatgtt cgcagtgcat aggagtcaag taagattcta gccagtatag
     9061 aaagtggaag tctggagcgt acctcataat ctgggaagaa ggagccttct gatttaaagc
     9121 caacaaaagg aatctcgata tgttccatca aacttgaatg acgattagca gtaagcaaaa
     9181 ttgtcccgat ctcagcttta taacagtttt cagctgcggt agtcaattcc tttgtttcac
     9241 cgtttagtga aacaaagatc acgatgtcat ctttggataa ttttctacta atgtttttta
     9301 tgatattggg atcagtatgc atctcacaat atttaccggt aagttgaaat ttgaccatca
     9361 tttcctgagc aatcaattct gaaaaacctc gggcaaaaat catgattctt ctagcagcct
     9421 tgattcgttg aatagcatct tcaatattcc cgctttctac catattgatg gtgcggatta
     9481 cctcttgctc atttttaaga atagcagtct ttattccttc atcaacttta tcaagagcgg
     9541 caaaattgat atttgtattt tctttttctt ttaacgaatg tttaaaagac gtaaacccct
     9601 catatccttt tttcttcata gtacgaacga cggttgctgt tgaaacgttg gcatattcac
     9661 ttagttttac aatggaatag gaggggattt cgagtatgtg actttgaata aaatcccaga
     9721 gatatttctc cgattcactt aattttgtat ccatattttg cctccgatta gttaggataa
     9781 ttctattgta aagcgggagt ttcctttact tcaagtttat ttaatatatt ttgtaaaagt
     9841 tttcaatgaa tttagtcatt tatgaaaaca tttacattcg atcttgatac ataatgaata
     9901 tgtaagaaag gaggagcgta aatgtcagtt tatacatgta ctttaaactt ggcaatcgac
     9961 ctttttatcg aaacagatga aatgaaacca tcccaagtca atcgaacaaa cgatgatgat
    10021 atacaagcta atggaaaagg tgtgaatgtt tcattgatca tgaagcaatt aggtatcgat
    10081 agtacagctt taggatttaa agcaggtttt accgggaatt atattgagga ttatttgcat
    10141 caaaaagata ttagcactga ttttattagc gtaccgggaa tgactagaat caacgttttt
    10201 acacaagtta ataaaaccaa tgaagaattc aaattggtta ataaaggacc tgagattcct
    10261 caggaacaaa tcaaaacttt tctaaatcaa atacaaaagc tgcaagctga cgattatttg
    10321 tgtgtttctg gcagtttgcc gcgaggggtc tcacccgaaa ttcttattga tatcagtcgt
    10381 atttgttttg aaaataaggt ccatctgatt attgattcaa gctatccgga aataattgat
    10441 tgtctgccgt atcagccgtt cttattaaaa ccaaacgagg aggaattgca atcttggtat
    10501 aatgtcgagc ttagtgatcg tgaaggatat attcattatg ctgaacgctt gattgccgat
    10561 ggagcgaaaa atgttctcct ttctctagga ggagagggag gaatattttt aaataaggat
    10621 atcgtttatt ctgggaattc tcctaaaggt aaagtagtca atacagcgtg tgctggtgac
    10681 actttgttgg gaacatttct tgcaggatat ttaaaaggtg aacaggtaga tattaatttg
    10741 aagaaaagtt tagcagctgg aagttcaacc gcttttcgta aaggattaac agatttttcg
    10801 gatgttgcag agttggaaaa acaaatcgaa atcaaagagg gagcgagtaa aaaatgacaa
    10861 cttacaaaat tgttgccgca acaggatgtc caacaggaat tgcccacact tatatggctc
    10921 aagaagcatt ggagcaggca gctaagcgca aaggggtatc tatcaaagtt gaaacccatg
    10981 gtcaagtagg gatagagaat gaattaacac cagaagaaat aaaaaacgct gacgctgtaa
    11041 tcattgcagc tgacaaagat gttcaagcag aacgtttcgc aggtaagcgt gtaatcgatg
    11101 tgcctgtcag caaggggatt aaagatgctg accaattgat cgaagatgcg ttggctggta
    11161 aaggttcaat cactgcagaa aacaaaacag ttgacgctgc tggtgagaaa gcggagatgg
    11221 gggttgcagc aggcattggt cacagtattt ataaaaatct catgaacggt gtttctcata
    11281 tgctgccgtt tgtagtggca ggtggggtaa ttattgcaat ttcgtttgcc atctggggtg
    11341 tgtactcttt tgatcccaat gatcctactt acaatccgac tgccgctatg ttaaagggtg
    11401 ttggtgatgc ggcaatggga atgatggttc ctattttagc agcttatatc gctgaaggaa
    11461 tcgctaaacg tcctggtttg gttgttgggt ttgttggcgg actgttagcc aattctggcg
    11521 gagccggttt cttaggcgga atttttgcag gtttcttagc aggctacttt atcttactat
    11581 tgcaaaaggt catgaaaggc ttgcctaaac aattggatgg tctgaaggcg atcttcttat
    11641 atccggtgat cggtgtcttg tttgttggtg tagtcatgtc tttattagca aatccaatgg
    11701 aagcagtcaa ccaaggaatg atgaatttct tggcaggatt ccaaaattct aatccattaa
    11761 ttttagggat tatcgttggc tgtatgtcag catttgatat gggaggtcca gtcaataaag
    11821 cggcttatgt tacagggact gcgttgttgg cacaaggtaa ccatacattt atggcagggg
    11881 tttcggcagc ttgtatcgcg cccccattga ttactggatt tgccgcactg ttttttggaa
    11941 aatatttcga cacgaatgat cgcaacgctg gtttagtcaa ctttatcttg ggctcaacgc
    12001 acattaccga aggagcaatt ccatttgcgg ctaaagatcc gctgcgtgtt ctgccaacta
    12061 tgatgctagg ttcatcaatt gctgctgtac tgacatatat gtttggtgtc caagttcccg
    12121 cacctcatgg aggttttcta gtattacctg ttgttactgg taaggttgca tgggtagggt
    12181 cgattttact tggttcaatc gttggcggag taattttagg gttgatccaa aaacgtagtg
    12241 tagaaaaacg tgaagcagaa gcagctgctg ttgaaatgaa agaagcaacc aacaactaaa
    12301 ggttattcta gaaagaagga tttcaaatga ctttgatcaa aacaaataaa gtttttatga
    12361 ataaaaattt tacgacgcaa gatgaggtaa tgaacttcct tgcagatcag tcaattgtct
    12421 tgagcattag tgaagaccga gatgcagttt acgaaaaatt gatggaaaga gaagaagaaa
    12481 gcacgactgg tatgatggat ggttttgcca tccctcatgc caaagatgct agtatcaagg
    12541 atgccgatat tgtgattgtt cggttaaacg gtaaggtcga ttggaaaagt ttagatggag
    12601 aaggaacgga ttttgtaatc gctctgttta ttccagattc agaggctgga acaacccatc
    12661 taaaattttt atctcaggta gccagacttt tgatgcacaa agatgtgaca gaaggtctga
    12721 aacaagccca gtctgctgaa gaaatagctg aacttttaaa ccaaaaatta gaagaaaact
    12781 aaaaaggaag tagtgaaatc atgcctttaa cagaaaaaaa gaaaacttat attcgtcaac
    12841 taagtaatga aagtggcatc atcagtgcgt tagcaatcga tcaacgtggt gccttgaaaa
    12901 aaatgatcgg taaatttcaa aataaatcag caaccgatga acaaatcatc aatttcaaaa
    12961 aaatcatttc ttctgaattg actccatatg cttcagcaat cttattagat ccagaatatg
    13021 ggttgccggc agccgaagtt cgcgataaaa atgctggtct gttgttggcc tatgaaaaaa
    13081 ctggttacga tgcttcaact ccaggacgtt taccggatat tttatctatt tggtctgtaa
    13141 aacgtctaaa ggagcaaggg gccgatgctt gtaagttctt gttatactac gatgtagatg
    13201 aatctgatga catcaatgac caaaagaagg cgtttatgga gcggattggt tctgaatgtg
    13261 ttgctgaaga tttgccattt ttcttagaat tggtttctta tgatgccaac gatcctgata
    13321 cttcttcgaa ggaatatgcc ttgaaaaaac cgcataaagt aaatgaaatg atgaaggaat
    13381 tttctaagcc tcgctatgga gttgacgttt tgaaagtgga agttcctgtt aatatgaact
    13441 atgtggatgg ttacactaaa aatgaagttg cttataccaa agaagaagct gctgcttatt
    13501 tcaaagaaca atccgagact accaacctgc catttatttt cttgagcgca ggagtaactg
    13561 ctgaattgtt ccaagaaacc ttgaaatttg caaaagaggc tggctctaca ttcaatgggg
    13621 tactctgtgg gcgcgcaact tgggcgaatg gtgtagaacc attcgttact aaaggtgaat
    13681 atgctgccaa agaatggatg cagacacaag gacgcaagaa catcgaagag ctgaataatg
    13741 ttttgaaaga aacagcaaca gctattaaat tgtaagaaag ctttaactat aaccttaagt
    13801 ttgtaaccta atgattttta ataatcaatt tgagctttta aataaagaat tggaagcgtc
    13861 ccgacgcttc ctttttcgtt tgtttttaag gcatgatagg ttctatgttg tcgttcaata
    13921 agcgcaagaa ggtcagcatt tttttcttag agtatgcaaa attgcttttc tacccaatga
    13981 tttaaaacgg agaggcaccc ttggatatgg tagatggtga agtaacgcat ttcttctgtg
    14041 atcacggttt ttttgtcttc ttgtagccag atttttaaat aattttcttc gatcagatta
    14101 tttaatctct tttgaaattg gatatctccg tggttgatga cgaataactt gtatacgaac
    14161 gtattcgtat agacatagtc gatcaattga gcgtatagat cctcgtagct atgtgtcgta
    14221 tcgtaggaaa tgatttcatc caagtcttgg aaaaagctat tttccagttt ttcataaaga
    14281 tcatagatat ccgtataatg tgtataaaat gtcgaacggt gaagatcagc agtttcggtc
    14341 agttcacgga tggtgatgtt ttgaattttt ttatctacta ataattttga taatgcttct
    14401 gctaacgctt tttctgtttt tttgacgcgg cgatcctctt ttttcatttt gcagcctcct
    14461 aatcaacata atcaaggaat tgtgtcggtt atccgacaat ttcaccgcta tctgaacata
    14521 gataaccgac accttgtcta ttataatata agtatagcat atcaatattt aaagaatagg
    14581 agaataagtg atggaattag cctttaagca actggaaaag aaagactaca aaaaagccat
    14641 tcaattcgcg attaaaggta tgcactttga ttggtatacc aagaacaaac tccttttgaa
    14701 tctttatgga agatactttt ggtatctaga attgaatcgc gcgacacaaa tcattgcttt
    14761 atatgcggat gatgagttgg caggtgtttt attagcagac atcaaaggaa aatcaaaaaa
    14821 gcatcattca tttagtcaaa agttatatgt taaactattt gatttcttac aaaatgcatt
    14881 tgtgaaagac agcaaaggcg ggtatgacga caccaatgac gaattgcttt cagactattt
    14941 aaaaaaacat tccccagatg gagaaattgt ctttttagct gccaaccctg atttaaagat
    15001 caaggggatc ggtagtaaat tattaaaaga atttgaacgc cgagagcaag gaaaagaagt
    15061 atttctgttc acggatagcg gctgtacgta tcaattttat gaacgacgcg gtttcgaacg
    15121 gaaaaaagaa aaagataacg tcatgaagat ccttaataaa gaagtagatc tccgctgtat
    15181 gttatatagt aagatcattc agtaatgttt tttactatca gaaagcagaa gtaccacgaa
    15241 ggtcaatgga aaaagcgagt aattgatcca tttagatgaa gataggtgta gtctcaaaga
    15301 tattagctgg caaagatatc cccagttaaa aatagatttc tgtatccaac aataaaaata
    15361 tttttgtatt tattagtata tatactttac aaaaatttgt atatatacta atttttttgt
    15421 aggatcaatg aaatgagggg aaaatatgcc aaaatataaa gaaatagcct tggctctaaa
    15481 agagaggatc gttgagggaa aatatcagga aggttctaaa ttacctgagc aagaaagtct
    15541 ggctagagag tttaaaacaa gccgtgttac tatccgcaaa gctatacaac tccttattga
    15601 tgaaggtttg ttatatacga gacgtggatc tggtactttt atacgaagca atatcagaca
    15661 ggacaatgag aatgtcactc aagtaaataa tgtatttggc acttcctcac aagaaaatac
    15721 aaatgtgaca agtaaaataa tccgttttga tgtccggttt ccgacagatt atgaaaggcg
    15781 gctttctgca acctacttcc gatccccttc ctgcaaatca tgggtataaa aaataggcat
    15841 attaagcggg ctaggaggag aaatattcac atatcatttt ttcattccgc aagctgcgtc
    15901 aagttatttg gaatttgtag aacagatgag agaggtagaa gcagcactac aaactacttt
    15961 tcaataagta ggaattatag aaattttact caaaaaatct tcattaaaaa atctgactaa
    16021 ataagttgag aaaacagaat atacttcaat atccagtgat ttataaatat cattcactca
    16081 tgatacccag ctccgtatca taagtgatgc ggtttttatc attgatccta aaaatcttag
    16141 caagacaaaa agtacgtaag aaagtaaaga ctaaatactt cggaggaaca aatgaataac
    16201 aatgataggt tattgcgatt gcgttatgcg attgatttga aagatacgga tttgattaaa
    16261 gcttttgagc taggcggtgt accactgact aaagaagaag ctcaggcggt actgaccaaa
    16321 gtacaggata agaacaaaga taatgcagaa aataatgtgt atgaaaaaac catcaacaac
    16381 caagtttttg acgcattcct aaatggcctg attctcttgg cgcgtggtcc gcaaaaagat
    16441 aagccgatag tagacacgcc gcaaaaaagc aaggaaatca aatatatcaa caatgtgttg
    16501 ctaaaaaaat taaaaatcgc cttgtctatg accactgatg atattttaga agtttttgct
    16561 gaggcagaac tctatccatc aaaaggcgaa atcggggcat ttttgagaaa agaaggccaa
    16621 cgaaatttta agccttgcgg agataaatat atgcgtaatt ttctcaaggg gttaggaatt
    16681 tataatcgga gaaaagtata aagaaatttc atgagaaatc aaaacaatgg ttgttttgat
    16741 gagagctagt ttttgtatga gattaaaata ttatcttaat cgaggagctg gattggtttt
    16801 atgaatgatg aatataagaa tgatgaagat aagatgcttt ttgaagagat agagaatcga
    16861 tgtaggctca attttgagct tcggggaaag atgtctttaa tacaacaaaa aaagtattta
    16921 gcgaataaat cagagtttac tctgggacat gttgaaaaat taatctcgga ttggattagt
    16981 agtagaagcg aatttacaaa aataaaacag ccgattaaat ttgacatgaa aaaacttttg
    17041 ttaaataaga gtgagatagg taatagagat caatatatta gagctaaagg gcaagaaata
    17101 attgattcat taggtgaaat gaggtcatac aattatttgt atgtaacaca tagagctgat
    17161 ggaatggtca ttactgtggg gaaatcttct tctaatgata tttttttaga tggagatctt
    17221 ttttatcaac tcaatacaaa tcacttgtct ggcactgaaa atataatttt aaggacggaa
    17281 tatggcaatg aaatatttgc aaaatatgat gagattttaa aaaattattt ggactgggct
    17341 tggataattc cggttgagtc aggagatgct aagaaactgg agagattatt gggcgatgaa
    17401 ctaattaata aaaaagtacc aattttaaat tattattccc atagacaata aaaggctatt
    17461 tttcatgata atactagtta taactgtaaa aatggaagaa aatctgggaa agaagttttt
    17521 aaataagtgt attgttcgat tttaataaat aataagcaat accatccgtc tgtgttgcgc
    17581 aacatcgtac tacaatgaat attttttcac aaaaagaaaa tcatccatcg aatgaatgga
    17641 aggttttagt atacacgagg ttaatgcccc ctcttttata atcagaggag tgaggttttt
    17701 tctatgaatg tttctatttt cttattgcta gtctagtttc tcttacgatg aaattgggat
    17761 tctattccag tttttattga tttgctgata aaataacttg aatggtggat ataaacatga
    17821 taaataagag aagaataaca aaacaaggtt gtgaaatgat tctcaaatga atataataga
    17881 aagaaaaagg aggaagaatc aatgaaaata gcaattgcag gcgctggcgc aatgggaagt
    17941 cgctttgggt taatgcttca ccaaagtggg aacgaagtat tattaatcga tggctgggct
    18001 gaacatgtcc aacaaatcaa agagcatgga ttacaagcaa attttaatgg taaagaggta
    18061 gaagcaaaac taccaatcgt tcttcaatcc gaagtagaaa aagaagatca agttgatctg
    18121 attattctat ttaccaaagc gatgcagctg gaaaaaatgc tgcaggatat ccaatcatta
    18181 atcaaaaaag atacagaggt cttatgtcta ttaaatggta tcgggcatga agatattatt
    18241 gagaaatttg taccaatgga aaatatctat attggaaata ccatgtggac ggcaggtctt
    18301 gaaggtcctg gtcaggtcaa attatttgga agtggttcag tagaattaca aaatttaggt
    18361 gatggaaaag aagcagctgc aaaaaaatta gcagacaaat tgtctgaatc aggattaaat
    18421 gctcattttt ctgacaatat tcactattct atttatcgca aagcatgtgt gaacggaaca
    18481 atgaacggac tatgcacaat cttagacgtt aatatggctg agctaggaaa aacatcgact
    18541 gctcataaaa tggtggcgac gattgtcaat gagtttgcca aagtagcagc agtagagaag
    18601 attgaactag atgtcccaga agtcattgca cattgtgaat cttgttttga cccggaaaca
    18661 attgggttac attatccttc aatgtatcaa gacttgatta agaaccatcg attaacagaa
    18721 atcgattata tcaatggcgc aatttctaga aaagggaaga aatatggtgt tgcgacgcct
    18781 tattgtgatt tcttgactga gcttgtccat gcaaaagaag atagtttgaa cgtaaaataa
    18841 aggagagatt gaatggaaac ttcacaaaaa atatcaccga aaatttttct gaataaagtc
    18901 ctcgctggta cagcaacagg aatcattatt ggattgattc ctaatgcagt attaggtgcc
    18961 attttaaaat attttagtgc gtatcccttt gcggtaacga tcgcacagtt agcagttatt
    19021 tttcaactag caacaccttt gatcattggt ggtttgattg ccttacaatt tggatttgat
    19081 ccgatgagaa tgatggttgt agccggagct tcttttgttg gttctggtgt agtcaaattc
    19141 acacctgatt tgggtgaaac gggtgtttac gttggtgctg gtacaggcga tattattaat
    19201 acgatgctga cagcatcgat tgctgtcggg ctgattttac tgattggaaa tagttttggg
    19261 tcagtgtcga ttgtgttaac accgattgtt gtaggtgtag gtgcaggttt acttggtttt
    19321 tatttattcc cttttgtaac agcaattact tctgcaattg gatcattgat taataacttc
    19381 actaccttac aaccgctgtt aatgtctatc ttgattggtt gttcctttgc ctttttgatt
    19441 atctctccta tttcaacagt agcaatcgga atggcaatcc aactagatgg tgtttctgct
    19501 ggtgcggctg cgatgggcgt agctgcaacc acttttgttt tagtagtgaa ctcttggaag
    19561 gttaataagt caggtgtgac aattgcgatt gctttaggcg ctatgaagat gatgatgcct
    19621 aatctattta gaaaacctgt gattcttatt ccttgtttag ttagtgcagt gattacagct
    19681 attcctgtcg ctctattttc tatttcaggt acgcctgctt ctgctggttt cggcgtggtc
    19741 ggacttgttg gtccgttggc ttctttagat gcagggctaa atggctttct tgttgttctt
    19801 tgttggttag tcatccctgt ttttgcggca ctaattactc aatttttatg cgagaaagta
    19861 tttaaactgt atgatcgaga agaagtcttc aaatttttag gctagtagat tttactaaga
    19921 aattttcaat caatggatgc gtaagtgaga tgtcaacaac atctgactta cgtttttttg
    19981 cctttttgag taggagattg atgctgacaa ctgaactctt cagtgccatt cgatttttta
    20041 aaatgtagaa gaaactcacc tttatgttat tgattgttca aaaggcaact agaataaaaa
    20101 tatgtgttaa aatgagaaaa aatttgattg ttggatgtat cgaataatca ctaaaaagta
    20161 ggagaagaaa atgatttttt tgcaaaaata tggtttttat tttttattat taggtgtgat
    20221 tagtgattta tcgacaccgt atatccttgg ccttttttat cccaaactga atcaaatgac
    20281 taccgtaatt agtgtgtttg gtgacgtaga tagtccagtc agacgtgctt tcttggtctg
    20341 gtcagttgta tctggacttt tttttgttct ttcgttgcct gcgttgtatc acctatttat
    20401 tggaacatca aaaacacttg caatacttgc tgttgcaaca gttggtttat atgggatagg
    20461 tgattgtatt tttactgggt tattcagtat caatacgaat gaaagctctt ggaatctatc
    20521 gacttggatc cataatatag gttctgggct aggctatgca ggctttctac ttttcccatt
    20581 gttacttgtt ctgctttatc gtcaaagtgg tcaaggtacg cttagccatt tttatcttgt
    20641 tttaacagtt attagtttac taattgcagg tctttacgga ttagcacgga ttccgtcgat
    20701 tagtcagttt gccttcttta agcaattggg cttctttcaa cgcttaagtt ttttctttaa
    20761 ctatttgccg atgatttgtt taagtttttt tcaactacga aagtagaaaa agcagtgtga
    20821 agctgattaa gtaaaagaat ttctctatgg gtaaccattc gtgtggatgt gttcgactaa
    20881 gaatagagat gaggagagcg aaaagcaagt tagtcttaac ttgttatgcg ttgtgctaaa
    20941 tttcaggtag tcattttttg aatgaataag aagaaaactt gaaagagggt gacttcatgt
    21001 tagatgtgga gcagatggaa atagaccttt ataatattat tttaaatcga aatacaagag
    21061 aatgggaaag aacacaattt gaagaggcaa aagaagacct agcaaaaatg tcgataaagc
    21121 aggtgataga aaaactagag gtcaacttgc gtccgctagc actacgagat aatttgacac
    21181 cagatgcgat ggatttttat ttaagaatca caggggagtc agcgagtgca aaggcttatg
    21241 attatgccaa acataagata acttcaccct tataccaaga acgggcgatt tttgccggtg
    21301 gctgtttctg gtgtatggtt gagccttttg aaacaaaacc aggtgtaatc tcagtgttat
    21361 ctggttatac aggtggagag accaaaaaac caaattacga tcaagtcagt ggtggatata
    21421 ctgggcatgt tgaagctgtt gagatcatat ttgatacacg tcaaataacc tatgaagagc
    21481 tgttggagat ttattggcaa atttcagacc ctacagatgc ctttggtcaa tttcaagatc
    21541 gggggaaaca atatcgttca attctatttg ttgttaatga aaagcaaagg caattggctg
    21601 aaaaatcaag agaacaactg attcaatcta accgatttag ccgtcctatc gttacagaga
    21661 ttcgccaagc aaagactttt tggccagctg aaaatcatca tcaacaattt tatcagaagc
    21721 aacctaaaag atataaaaag atcaagcatt cgcatcagct tcatttattt tttcagcgac
    21781 atagatagag gaaggttgtg gcgcaaagcg ttcagctccg acaaaataat gagcaactga
    21841 caaaaatagc ttttcatatt tttggacagt tatgaattat ttcgaggagt tgctttgaga
    21901 acaccgagga aaaggttgtg gcgcaaagca ttcagcttca agtcgcaagg aaacaggagg
    21961 tatgacaagt cttttgtcac aactcctgtt tttttactag cctttatttc catgtgaaag
    22021 agaattcgaa ttgtagaatc ttgatttttc tatggaattt tgtagagaat catcttgata
    22081 attaatagac acctaatcga attccacatt aatgatgaac taaattccgt ataaaagcgc
    22141 aaacaataac tata
//
LOCUS       DAFEPQ010000040        22026 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.040, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000040
VERSION     DAFEPQ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.040"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(61..1371)
                     /locus_tag="LP543_001683"
     CDS             complement(61..1371)
                     /locus_tag="LP543_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBL2321850.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="LP543_001684"
     CDS             complement(1383..2801)
                     /locus_tag="LP543_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2321851.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="LP543_001685"
     CDS             complement(3041..4609)
                     /locus_tag="LP543_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBL2321852.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="LP543_001686"
     CDS             complement(4613..4894)
                     /locus_tag="LP543_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBL2321853.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="LP543_001687"
     CDS             complement(4891..5814)
                     /locus_tag="LP543_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HBL2321854.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="LP543_001688"
     CDS             complement(5811..6689)
                     /locus_tag="LP543_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBL2321855.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="LP543_001689"
     CDS             complement(6676..8013)
                     /locus_tag="LP543_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBL2321856.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="LP543_001690"
     CDS             complement(8016..9119)
                     /locus_tag="LP543_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL2321857.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="LP543_001691"
     CDS             complement(9193..10701)
                     /locus_tag="LP543_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL2321858.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="LP543_001692"
     CDS             complement(10667..11359)
                     /locus_tag="LP543_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBL2321859.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="LP543_001693"
     CDS             complement(11381..12355)
                     /locus_tag="LP543_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2321860.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="LP543_001694"
     CDS             12655..13527
                     /locus_tag="LP543_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2321861.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="LP543_001695"
     CDS             complement(14109..16307)
                     /locus_tag="LP543_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2321862.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="LP543_001696"
     CDS             16539..17531
                     /locus_tag="LP543_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2321863.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="LP543_001697"
     CDS             17591..19012
                     /locus_tag="LP543_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBL2321864.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="LP543_001698"
     CDS             19005..20504
                     /locus_tag="LP543_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBL2321865.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="LP543_001699"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="LP543_001699"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBL2321866.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6668 a         4494 c         3824 g         7040 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaagggg gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaa
//
LOCUS       DAFEPQ010000041        21942 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.041, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000041
VERSION     DAFEPQ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.041"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(580..1596)
                     /gene="tsaD"
                     /locus_tag="LP543_001700"
     CDS             complement(580..1596)
                     /gene="tsaD"
                     /locus_tag="LP543_001700"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBL2321867.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1593..2057)
                     /gene="rimI"
                     /locus_tag="LP543_001701"
     CDS             complement(1593..2057)
                     /gene="rimI"
                     /locus_tag="LP543_001701"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBL2321868.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2064..2606)
                     /gene="rimI"
                     /locus_tag="LP543_001702"
     CDS             complement(2064..2606)
                     /gene="rimI"
                     /locus_tag="LP543_001702"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBL2321869.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LGVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2590..3312)
                     /gene="tsaB"
                     /locus_tag="LP543_001703"
     CDS             complement(2590..3312)
                     /gene="tsaB"
                     /locus_tag="LP543_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBL2321870.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(3503..4483)
                     /locus_tag="LP543_001704"
     CDS             complement(3503..4483)
                     /locus_tag="LP543_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2321871.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(4507..5991)
                     /gene="galT"
                     /locus_tag="LP543_001705"
     CDS             complement(4507..5991)
                     /gene="galT"
                     /locus_tag="LP543_001705"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBL2321872.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6003..6992)
                     /gene="galE"
                     /locus_tag="LP543_001706"
     CDS             complement(6003..6992)
                     /gene="galE"
                     /locus_tag="LP543_001706"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBL2321873.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            7240..7407
                     /locus_tag="LP543_001707"
     CDS             7240..7407
                     /locus_tag="LP543_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBL2321874.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(7469..9280)
                     /locus_tag="LP543_001708"
     CDS             complement(7469..9280)
                     /locus_tag="LP543_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBL2321875.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(9277..9642)
                     /locus_tag="LP543_001709"
     CDS             complement(9277..9642)
                     /locus_tag="LP543_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBL2321876.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFARKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(9805..10200)
                     /locus_tag="LP543_001710"
     CDS             complement(9805..10200)
                     /locus_tag="LP543_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBL2321877.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(10218..11180)
                     /locus_tag="LP543_001711"
     CDS             complement(10218..11180)
                     /locus_tag="LP543_001711"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBL2321878.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(11180..11392)
                     /locus_tag="LP543_001712"
     CDS             complement(11180..11392)
                     /locus_tag="LP543_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBL2321879.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(11413..12111)
                     /locus_tag="LP543_001713"
     CDS             complement(11413..12111)
                     /locus_tag="LP543_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBL2321880.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12131..12673)
                     /locus_tag="LP543_001714"
     CDS             complement(12131..12673)
                     /locus_tag="LP543_001714"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBL2321881.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(12805..13809)
                     /locus_tag="LP543_001715"
     CDS             complement(12805..13809)
                     /locus_tag="LP543_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBL2321882.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALTSGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(13806..14795)
                     /locus_tag="LP543_001716"
     CDS             complement(13806..14795)
                     /locus_tag="LP543_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBL2321883.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(14792..15598)
                     /locus_tag="LP543_001717"
     CDS             complement(14792..15598)
                     /locus_tag="LP543_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2321884.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            15764..16720
                     /locus_tag="LP543_001718"
     CDS             15764..16720
                     /locus_tag="LP543_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL2321885.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVRKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(16797..17315)
                     /locus_tag="LP543_001719"
     CDS             complement(16797..17315)
                     /locus_tag="LP543_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL2321886.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(17403..17552)
                     /gene="rpmG"
                     /locus_tag="LP543_001720"
     CDS             complement(17403..17552)
                     /gene="rpmG"
                     /locus_tag="LP543_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL2321887.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            17780..18226
                     /locus_tag="LP543_001721"
     CDS             17780..18226
                     /locus_tag="LP543_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBL2321888.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(18421..20316)
                     /gene="asnB"
                     /locus_tag="LP543_001722"
     CDS             complement(18421..20316)
                     /gene="asnB"
                     /locus_tag="LP543_001722"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBL2321889.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(20641..21615)
                     /gene="bsh"
                     /locus_tag="LP543_001723"
     CDS             complement(20641..21615)
                     /gene="bsh"
                     /locus_tag="LP543_001723"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBL2321890.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT         6583 a         4655 c         3757 g         6947 t
ORIGIN      
        1 aaatatcagg gtcactgctt attttcctcc ctaaaacttc tgaaagatcc ggcagtttta
       61 gatcactgga caagactcaa agattttcta tgtgtgtttc agttatgtaa tattcactgt
      121 ttgcaaaaaa tactgttaga ttaactagat gaagcgtctt gtcctcatcc gatctagatt
      181 attcgatatt cctcgacaat aaacgattac tcagtgaggg caatcagagg aacggataat
      241 gataaataag ctgtaaaaaa tagcaaaata acaataatga ttaaaagtat gtaaaaaata
      301 gggctttgac aaaaacgtgt cacagcccta tttcgattaa acggtgtgta cctgtcaact
      361 cttcggtcac aagtcataat gttcataaac atgaggagct gttttttgaa attgttcctt
      421 gtgaatcaga gttaaacgac aggcttacga actcttgatt aaacggcgtt caaaagcttg
      481 catacgcggt aataagaaca gccaggtcaa aaacatgaga agccgttttt cctgggctgt
      541 tcttattact tgatgcaggg cagctttttc acaacctctt tattcagata aaaccaagct
      601 tggatcagca ttcaactgat agctagcaaa atttcttttt tgcatttcca catgtgctgc
      661 tgctccaatc attgcagcat tatctccaca aagtcgaaga gggggaatca ctagttctac
      721 ctcagggagt ttagttgcca aagcatcctg caatccttca cgcaaccctt gattggccgc
      781 tacacctcca gctataatca gttgcttgac tggataattt tgacaagctc gaagtgtttt
      841 agtaatcaat acatcgatca cacttgcttg aaagctagct gctaaatcat ttttgtctag
      901 atcttctcca cgctgttgtg cattatgcac caaattgata aatgcacttt ttaatccgct
      961 aaaactgaaa tcataattat cttcatggat catcgctcgc ggaaaatggt aattatcttt
     1021 tccctgatga gctagctgat cgatttcttt tccgcttggg taagaaagac ctaatactct
     1081 tccaactttg tcataagctt ctcctgctgc gtcatctctt gtctccccaa tgatttcata
     1141 tgatccatct tcttgcatat agactaattc tgtatgtcct ccgctaacaa gaagtgccat
     1201 caatggaaac tggaatggtt taaccagacg ggctgcatag atatgccctg ccatatggtt
     1261 tacaggaata agcggcaatt ggtgtgccca agcaaaagcc ttagctgcag aaataccaat
     1321 cagcaacgag ccgacaagtc ctggtccata agtgacagcg actgccgata aatcttctgc
     1381 cgacacacca gcttctacca gtgcatcttc caagcataag gtgatttgtt ctacgtgatg
     1441 gcggctagct acttcaggta ctacgccacc aaaacgttta tgactgttta tttgagaagc
     1501 aacgatgtta gataagatct ctgttccatt tctaacgacc gctacactcg tttcatcaca
     1561 acttgattca atcgccagaa tcaattcttc attcatttca tcgtctccgt ttttaacttt
     1621 ctgctcataa tgatcgcatc ttccgtcgga ttgcgataat atgatttccg tttacctaat
     1681 atacgaaact tttctgattc ataaagtttt cttgctgctt cattagatag acgtacttct
     1741 aaaaaaacat gcagcgaatc ttttatttgt tccgtaatca gtaattgtaa caaatgccgt
     1801 gcatgccctt ttccttgttc tgaaactgca acagcaatat ttgtgatctc gacttcatct
     1861 attactttcg agtatccaat aaagccacag atctggttat cttcagctaa aagcagataa
     1921 tcagagtgag gctgttggat gtctgccaaa aactgcattt tcgtccaagg tgaaccatgt
     1981 ggataagcat tttcgctaat catccatacg cattctgcca agtacgcttc atcaaaatct
     2041 ttttttctgc aaatcaacta atctcactca ttttctaaaa gttttatcat atccaacgca
     2101 tcttcttttg tttcagtgta atatcttttt ttgatcgttg ttgcttgaaa ccccaatttc
     2161 cgataaagtc tttgtgcatc ttgattactc atacgcactt ctaatgacat cgtctcacag
     2221 cgattcatta tagcaaagtt ctcgatttcg tcaataagca gcgaaccgat tcgtttacgc
     2281 tgatatgccg gtcgaacggc aatattcgtg atatggcaat ctaaaccgaa cgctcttgaa
     2341 ccgataaaac caatcattct tccattatca aagataccta gatatagatg aggaagcggt
     2401 gacttcaatt ctgatataaa ggcacttttt gtccatggca attcgccatt atagacttct
     2461 cgttcgacac ccagcagctc tttgatatct tctgtagtca attctcttag tacgtaacga
     2521 cgatcgattt gttcgactgt tttttctgta taaatcattt tatcttgaaa aaagcttctg
     2581 gcatacgctc taaatttttt caacataact ttcgtctcca ggtgtgtgcg tctccaacca
     2641 tttttcttct gcttctactc gttttaaata gcgaggaaga aaagcttgtt tctctgcaac
     2701 tggttcaact aaagcaccta attgagccac agttacacca cttggaatat cccatgctgg
     2761 aactgtatta atcatcgcac taggcaatat ttcctttatt tcagagaaga atttgtgaca
     2821 atcactcccg acgaaataaa tagcttcatt cagcttttgt agttgtgtga acaaatcaga
     2881 agcagaaata tgtacatctt caagtacagt ttctaatcta ttttctttcc agcggtatgc
     2941 cccagcgtat acattttttc ttcgagcatc aaacatgggc acaataatat tatccacacc
     3001 gatgcaatta gccgcgatgg ttttcagact agatactcct actagctctt tatctaatgt
     3061 ataagcaatc gtttttgctg tcgttacacc tatacgaagc cctgtataag aaccgggacc
     3121 atcagaaacc gcgattcggt caatgtcttt tggacttatt tgaagatctg caaacaattg
     3181 gtcgattgca ggcatcagtg ttacactgtg gttgcgtttg atatttgttt gtatttgtcc
     3241 aagaatttgt tcgtcttcta ccacaccgat cgccaaagtt tggtttgcag tatctattcc
     3301 taatgtaatc atatatttga tcctttctgc tatgacattc tcgccttcca ttgtagcata
     3361 cttttttatt ctgaggatga aaataccaaa ggcaaaacta gtacaataat acatgattat
     3421 ctgctggcgg gcaaattttg ccccttcctg aaacacaaaa aagctgcggt atcactccat
     3481 tttcccacag cttttccatc ttttatttcg ttgattctcg aataatcaac tctgtttcca
     3541 caataatctt tcttgaaacc ggtgctttgg aattacttaa ttgaagaatc gtctccactg
     3601 ctagctctcc catccattcg gtataaactt ttacagtaga aagcgagggc gttacatatt
     3661 tcgctacact gacatcattg aagcccatca cagaaatatc ttctggtacc actaaacctt
     3721 tttcttgaat cgctttcagt gcgccaatcg ctaatgcatc attagctgca aaaagcgcat
     3781 caggaacatg cggattcgtt tttaaatact gcttcactgc ttgatatcct gactcgacag
     3841 aaaaatctgc ttcgataatt ggaccttcgt cgtagccttt ttctgccaaa acttgttgaa
     3901 atgccataat tctgggatct tttagccgct gatggttttt cttcgtatat tctgttccag
     3961 caagcatagc gatatgttgc gctttatggg agacaaagtg ttcaatgact ttttgaacac
     4021 ttgacttaaa gtcgacaact agagagtcga ttccttgaga agtgccgtca gaatctacca
     4081 aaagcaaggc attttgggat cctttcattt ctaataactg agcttcgtcg aattttccta
     4141 gtgcgatcgt tccatctgcc ggaagatcac taatggaatc ccacgtttct ttcaataaag
     4201 taacgtttgc ttcttctgcc tttttttcga ttcctaggcg aatagataaa taataaagat
     4261 cttcaagctc ttccgtatcg tcgtaccact ggatcaggcg aagcgtttgc ttagtatttg
     4321 ctgcattttt acgatgtttc gtataattta agcgttctgc tgcttcaaaa attttttgct
     4381 ttgtggctaa gccaacggaa agatcaggat catagttgag aactcttgaa acagtagcag
     4441 gagaaactcc tgccaactgc gcaatgtctt taattgttgc catttgtctc tcctcctttt
     4501 ttacgtttat aaaatcgcag taaatcgttc aaacgctgcc tgtccttttt catctcgctt
     4561 gaatactcca gcgtcttcca gtacacgtgc aaaaacctga cctacagatt tttgtacgac
     4621 atcggtgacc gtatctttcg tcaaatcagg atgttctttt tttaattgtt ctgcccacgg
     4681 acgatgatat tcagccatct ggttttcttc tcctaataag aaatgctcta cttctactag
     4741 ttctggtttc aaacgaggtg gtaaaacagc aagccccatt acttcgatca acccaatgtt
     4801 ctcctttttg atatgttgga catctttatg tggatgaaaa atcccatcag gatgttcttc
     4861 tgaaacattg ttgtctcgaa ggacaaggtc caactcaaag taattgtccc ttcttcgtgc
     4921 aattggcgta atcgtatggt gtggtgtacc atctgctgaa aaagctctga tagatactgt
     4981 ttcatccgaa tatgctttcc atttttccaa tactgagtta gctgcttcaa taagcaagtt
     5041 tttctctgtt gattgcaaac ggatcacaga catcggccat ttcacgactc ctgcgatgac
     5101 ttgcggataa tcttctagtt tgataagttt ttctatcggt gcttttgcca taggaaattc
     5161 atgacgtcct gcctgataat gatcatgaga aagaatcgat cctccaacaa ttggtaagtc
     5221 agcattcgaa ccaacaaagt aatgaggcaa gacttcagta atccgaagca gtctagcaaa
     5281 tgtttccttt gtgattttca tcgggcggtg ttgttctgat aaaatgatcg aatgttcatt
     5341 gtaataagca taaggggaat attggaatcc ccagctttca ccgtctagat tcattcgaat
     5401 aatccggtga ttggtgcggg ctggatagtt caagcgacct ctatatcctt cgttttccat
     5461 gcaaagcatg caggtaggat agtctacttt taccgcctcg cgttctgctg cgatttgttt
     5521 tggatctttt tctggcttag ataaattgat tgtgatttcc agttcaccat actccgactc
     5581 tgccggaaat ttgatgtttt ttgcgatggc tcttgttttg atgtagtcat ttttcttact
     5641 taaattaaag aaatactcag ttgcttcctc tgggcttttc gaatagtgct gtgcaaagaa
     5701 tgcattgacg actgaaggtg gcggtgtgag aaaatccatt aattgcgctt caaaaatctc
     5761 ttgttctgct ggcaattctg aaagaacacc attttctctt gccttagcga caagctgatc
     5821 caataagtct acagattctt ccttaactgg tcttacttca atgctttcta gagcatcttc
     5881 tcctaccatc cctaaaagac gattctgtaa atataagcgg tccatttcca tccatccgcc
     5941 agcttcgatc gcaagtgtgg caaaatcaca aatcgtttga ctgattgaca tatctcattc
     6001 ccctattctt cataaccatt tggatggctg acatgccaat cccatgctgt tttgataatc
     6061 gctttgatat ctgtgtattc aggtttccag cccagaatct ctctcgcttt ttcacttgaa
     6121 gcgactaatc ggctaggatc tccagctctt cttggtgcta tttctgctgg aatttctttt
     6181 cctgtcactt ctctagctgc ttctagcatt tccttgactg agtatccttt gctgctgcct
     6241 aaattgaaga agttgctttc atttccttct ttcaagtatt ccaaagctaa aatatgtgca
     6301 gcaatcaaat cttctacttg aacataatct ctgatacatg ttccatctgg tgtatcataa
     6361 tcgtctccat aaacagccaa ggcttttctt tgaccaagag ctacttgtaa aatgattggg
     6421 acaagatgcg tttctggtgt atgatcttct ccgatagatg catctgcttt tgctccggct
     6481 acattgaagt agcgcaaagc gacatagcgc atgccatatg cttggtcaca ccacttcatc
     6541 attttttcca tcatcaattt actttcacca tacggatttt taggattagc tggtgtatct
     6601 tctgtgattg gagactcttt cggctctcca taagttgctg cagtagaaga gaatacgatt
     6661 ttattcacgt tgaactcgtg catgacttca agtaagattt gcataccgta tacgttgttg
     6721 ttgaaataca tcaatggttt ttcgacagat tctcctacta aagagcttgc agcaaaatgg
     6781 attactcctt caatcggttc cttttcaaat acggaacgta gaaattcttt atcccggatg
     6841 tctccttcat aaaaatgagc atctggatgt actgcttgtt tgtgacctgt gagcaaatta
     6901 tctaccacag ctacttgata ccccttttga actagctggt ctacagcgtg cgaaccgata
     6961 taaccggctc cgcctagtac taaaattgtc ataaaaaaca ctcctttgat tagtaaaatt
     7021 gtgtttgttg attctatata atttaaaaaa cgaagtttac ttttatggtg aaaacaaaca
     7081 aagtaaaatt tcgtaaattc atatggtaaa tttataataa aacatttagt aaacattttc
     7141 aaggattttt tgcaaaggtt tacaaacaaa cgtaaaacta gccttttttt ctcttttgtt
     7201 ccataataga tataacaaat aataaatgag gtggattcaa tgaaaaaatt tgtatctgga
     7261 atattagtag gaagtctggc aacagccgct gccgttgctg gtttggttgc ttctgtgaaa
     7321 aaaacagtga tcgatccaat tgacgaaaaa gaagcaatga tcgaagaaaa ccgtaaaaaa
     7381 gcaatgcgta aacgcgtttc tcgttaataa ttatttaaaa atacgcatga agactttcta
     7441 aaaaacagat aagtcatcat gcgtattttt atttgtcagt caatataagt tgtgctattt
     7501 tatctgcctg tctcagattc catggatctg tataaggtct agctactgct gcagttagcc
     7561 aatccacacc ttcaacaggt attccatagc aataaaagtc agactggatt tcccctgaag
     7621 catcaatcac gcgattcgta cctcgctcaa catctatagc ttctgaatga taacttcctg
     7681 tattatctga aaatgaaaaa gggcgaacaa gtttgttttc cttcaaattt tttatgacag
     7741 gattacttga acgagaaaag cttgtttgtg gcaatcgagc ttcaatcaaa tagcgactgc
     7801 tgatttcttt atcttgtaga gaaataacat agcggttgtg cttttgctga atatcaaatg
     7861 gcggttcgat caaatgaaaa atacccgcat ctatcagtgc tgctaattct ttcgtccgaa
     7921 tgattggcgg accgatcgtt aaaaagctat ttagatgagt aaaccatccc catagtattt
     7981 ctttgtattc cttagcagaa aaaacgtgcc attcaatagc ctgccggatt ggatcacgcc
     8041 agtcttttaa tgcatcaaat gttgaagcaa atgctcctgt gcaattgcct ttttccgctt
     8101 cttttatctg cccttcaata aatattcggc tagcttctga aaagggctca ttctgatagg
     8161 caagaggagt ttctaaatac gaccaatccc atctataagg gattagtgct ggatgctttc
     8221 cgagacactc ctctgaagag agggagagaa aatcatgctc aaaatttttc tttggaatcg
     8281 gcaagtgata ttcttcgatg attttcttgt aataaaacaa ctcaacgtct tttttcagat
     8341 agtcaaaaaa tgtttcgcca gtcaaaaatc ccgactgatg ccattgctcc aggttttctt
     8401 ttgttagtaa ccgtggccat gccattgctc cgccttgctt ttgatttctt cctcgagggt
     8461 aataagggag cccccttctt gatccactgt atacaatcgg ttccttacct gatggatgat
     8521 agaccagttt tcccttttcg ttctcgaagc gccctcctct tgatactgta aacaatccga
     8581 cataatcaaa aaaacttaag cctagacctc ttaaaaagac attttcttta ggcggtatct
     8641 tcgaaagatc tacatctgca ggattagaag gaagctgata cagtaaaaca tttttctttg
     8701 cgtagtctac ccacactctt tcttcttctg tctcatcatt ttcccagtga ccgcttgcta
     8761 aaacaagctg atcgatagaa tagctttgct gatctgtctg taaaagataa ccatcttttc
     8821 ttttgcttac tttttttact aaagaataaa caaactgaat agaagctgga aattcttctt
     8881 gcaactttga gaaaaaccag cgttgataga gtccgtataa acatcgagta gattgctgat
     8941 ctttttccag acgattgatt tcttctataa ttagttgctt atttttggtt accgtctgtt
     9001 cgatataatc tgtagcaggt cctttactcc attgaaacaa gttgggtccc tgtgttacca
     9061 atcctccact ggacaatgtt tcatctgtaa aaagcgtcac ttgttgagaa acagaattca
     9121 ttaataactc cggtggctga ttcaaccgcc aaactcgacc tccaggacca ttgggatcga
     9181 aaagaaaaag ttcaagagaa tgtctgtcct gattattatg gagaatcctt tctgctacac
     9241 ttaatcctct aggtccggca ccaatgatcc ctattttcat ttgcatgcct ccatttcgat
     9301 aaattttttc aataaaacag catggttctg atgttcattt tttgcaccat aaacaaaagt
     9361 aatgactgga tgttctttca taagttgact gagtttaaaa atagcttgct tcttttcttc
     9421 ttcgaaaatc aattcttgct ggtatttcct tgcaaatgaa gcaaatcgtt ctggtacatg
     9481 gtcaaaagtt ttgcgtgctt cattactagg tgcaatttct ttcaaccaca gatctaactg
     9541 cgctttttct tttgaaattc ctcgtggcca gaatctatcc acgagtattc tatagccatc
     9601 ctctttttca gcatcaaggt aaacacgttt taatttgatc atagttgttt ccttctttct
     9661 accttttcgc ttagcttata tatgtagata atagcacaaa ataaaaaaaa tgaacggcac
     9721 aactatccat tgcgccgttc atgtgatctt ggtcaaaatc taatgattga ttcggttttt
     9781 aaagctgtca tacaatcatc tgttttatac ttttttactt ttgatttttc cagaccatcc
     9841 gtcatatcca cccttaagta aataaatctc ttgataacct gctttacgta atttatttgc
     9901 tgtacggatg ctcaaacttt tcttttgatc gtaaatatag acaggctggt cttttcgaat
     9961 agatcctaat gaatctttca aaacagtgta agggatgttt cttgcaccta aaatatgccc
    10021 agcatcaaac acatcttttt cgcgaacatc gattacttgt gcttttctca ttccttctcg
    10081 aaattcttct tctgtcaaag tcgttgctga acgtttggcc attactcgta gatagacttc
    10141 ccaaaggatg attgcaagca caatcaatag caaaatgata ttaattaccc ataaaaccat
    10201 gtaatgcctc cgttttttta agcaaatttc aaggctaatg aggcagcacc aatgacacca
    10261 gcttcattac caagctctgc taatttgatt tttgttgatt ctcttacttg cgggaaagta
    10321 aattctttaa aatatttctc tacacggcta cgtaaaaatt ctcctgctgc cgatactcct
    10381 ccacctaaca cgatacttga cggatttaat gtattcccca agtttccgca agctaaacct
    10441 aagtagaagc atacgcgatc aacaaccatc aatgcaaacg gatcgtcttc ttgagccatt
    10501 tcaaagatat ctttacttgt tacttcttct ccgttgtcta gcattgcttt cagttttgaa
    10561 tcccctgcgt attcttctgc caagaaacga cctaggcgta ccacacctgt agcgctagat
    10621 acagtttcta aacatccgcg tttaccgcaa gtacattcga acccttcagg atccacagtg
    10681 atatgtccga tttctcctcc agctccgctc acgccgtgaa tcaaatttcc gccagcaacg
    10741 atacctccgc caacacctgt tcctagagta acgaatacaa cgtctggatc gttttctcca
    10801 gcacctttcc atcgttcacc taaagcagca acattcgcgt cattatcgat agcgaatttg
    10861 attcctgttc ctgcctcgat cagttttttc acaaattgaa cttctgtcca gtttaagtta
    10921 tacgcaccaa taaccgttcc agcttcgctg tccacgcttc ctggggtacc catgccgatt
    10981 ccgataaagt cttcggcttt catattatat aaattcaaac gatgattgat tgattcaatg
    11041 atttcaggaa cgatatgctt accatcatcc aaaatattgg tatcgatgct ccatttttgc
    11101 tgaatctctc cttctggtgt taaaattgca aattttgctg ttgttccacc taaatcaatc
    11161 ccaataatct tcttgtccat tacaaatttc ctttctttcg gctttcttca attcggtgct
    11221 ctctttttaa tatgcgccac gctgtcatgt atgtatcatg atcaatcaaa cggctatcat
    11281 acagattacg caattcgata gtcattaatt caatgtcata gatgcgtgca cccacataga
    11341 taaagatgcc aaattttttg aacagctgct gtacatcata taagctctcc atctttaagc
    11401 acttccttca tattatacaa gtaaaccata tttttttaaa cccagtaaaa tacatattac
    11461 aaaaagaaat acaaaaacta gtgtagaaag aatcctttgg tgtatcgaat agcttgtctg
    11521 ttgtttcggt agacccaaaa cattccctaa cactaatccg ccaattagcc caccgacatg
    11581 tccccaaaca tctacagagg tatccagaag accaaaaaca aatgtcaaca caataaataa
    11641 aagaaaatgt ctgactagct gctggatcgc tgggttatgt ttaaaatgaa accctaagat
    11701 aaacaacgca ccgaacaaac cgaatatcga agtacttgca ccaccagaca gaacattcgc
    11761 ttcgttaaaa gcaaagcttg ctgtatttcc cattatcccg ctgaacatat aaatcaagaa
    11821 aaaacgccaa tggccataga ttgcctcgat ttgctgcccc ataaaataca gtatcagtga
    11881 gttcattacg aaatgcatca gtccaaaatg gatgaagatt ggtgtcacta aacgccacca
    11941 ttcattgaag tgaacaagat aaggaccata cattccggca taataaggga tctcaagtcg
    12001 cggaaataat tccatcatga ggaagaccgc tgtctgtatc cctaaaaaag cataggtcca
    12061 taacggtcgt cttaaccatg cacgcaattt tatttgctgt tgataattca tgaaaacctc
    12121 ccatttcatt ttagtcaata aacaattgtt ggatcttctg atcaaagctc tccggttgcc
    12181 attgttcatt caattgttca cgaaaaacaa ggctacatgt ttttttatga aaattattta
    12241 aataccggtc gtaataacca ccgccaaaac cgattcgata accggctcta ttgtagataa
    12301 tacctggtac aatgaggaga tcgatcatct ccggcaaata gagcttctct gattgtggct
    12361 caagaacacc aaaactcgaa cgtacaagaa cggactgttc atccatttca taaaacgcca
    12421 tctgacgttt cggcaaagtt ttaggtacaa ctattttctt ttgttctttt tctgctcgtt
    12481 ggataaccgg ctgtgtatcc agttcaaatt cattcgataa agtcgtccct atcacttttg
    12541 ctgacttcca gtactcgctg gcgaatagct gtttatagat tcgctgttct ttttcctttt
    12601 tcttgccact tttggccaga tttttcagct tttcgattcc tttccggcgt aatcttactt
    12661 tcaattggtc caaaaactca cccttccttc tctcataaaa tggtatagga aaaaaagaaa
    12721 aatagcaatc gaactttcaa ataaatgcat aaatagaata aaaagaagac ctagaaatga
    12781 ttggccttcc ttttttaatg aatttcaact gttcagcgcg tgtttttgtc tgataaaaag
    12841 tgcatacaga aaatatggcg caccaaaaag ggcaatcaaa attcctgtag gtacttcccc
    12901 acttagtaga atcactctag caaccatatc tgcactaagc agaaaaatgg aaccgaacaa
    12961 accgctcaga acaaaggtcc agttattttc tttacgtacg actagcttcg cagcatgagg
    13021 agctagtagt cctacaaaag ataaactacc tgtcaccgct acactaccgc ttgtcaaagc
    13081 aacagctaac aacaaaagga ttttttgact tttagccacg ttaaggccta atccttgcgc
    13141 gcgttcttga ccaaatgata gcaatacaat cctctgtgca taaagtcctc ctagcggaat
    13201 taagataacc gcccatggaa gcaatgccca aacataaggc cagctcgcac cccagattga
    13261 tcccgctaac catgcattca caaatccata attttcctta gataattgga tcgtgcatag
    13321 caatgttaat gcagaaaggc cggcattaac ggcaataccg gacaataaga ttcgattcat
    13381 acccaaaaag ccatttcgat cataggccgc aagatagaca cagcaagcag ctaaaacacc
    13441 acctagaaag gctacaacta gcaatagaaa cggcagcgaa tagtgcgaaa agaaacctaa
    13501 ataaagcatc actaaaaatc ctgctccggc attaatgccc aatatactag aatctgccaa
    13561 ttcatttctt gttactcctt gcaagatata accagaaacg ccaagacaag ctcctgctaa
    13621 taaactgatc agtaatcgag gaactcgaaa ttctctaaca atcagcgcca aagaagatga
    13681 ttctttccct atgaacgtgg caaaaagctg atccaggtaa ataaaaggcg taccaatcat
    13741 taaactacct atagacagga tgacaagtac aaaaagcaag aaatagaacg tccaatatat
    13801 ttttttcacc gttgttccct ccttacttgt atcagtaaaa acggaacgcc aatcaatgca
    13861 atgactaatc caaaaggtgt ttcaaaaggg ggattgatca tcctcgctgt aaaatccgcc
    13921 aacataacca atccaccgcc aactaaaaaa gagacaggta gaacataacg atagtccgtt
    13981 ccaaccattc cgcgaacaac tggcggaacc aacagcccaa taaatgaaac aggtccgact
    14041 aacgaaacgg caatggctgc taacactgca actagacaga aaacaatcaa ccgcgttcgt
    14101 ttaggatgac ttccaagagc taatgcatgt gtttcactca aatgaatcaa agatattgct
    14161 tttcctaatg aaaaagaaac caaaacagca agtatatata aaggaaacaa tactgctaat
    14221 tgtttccatg tgatattggc agctccgcct acaaaccaaa aagcaagatc ctgctgcaga
    14281 ttaaagaatt gggtaagtgc ttggctgata gcagtaaaaa aagaactaat agctacgcca
    14341 gctaaaatca gctgtaaagg attcatgccc attttcgaaa actgcaaaaa accaaatacg
    14401 agcaaagtta cagcaaaaga acctgccaat gacattagaa acacagaaaa atcagaagct
    14461 ttaggccaaa aagcaaaact aagggccatt gccaacgaag cacccgcgtt gattcctagt
    14521 aagcctgaat ctgctaaagg atttcttgtc actccttgca tcaatgcacc gctcaatgca
    14581 aaacaactcc ctacaagaaa tgctcccgcc acacgaggca aacgaatatt tcgtatgatt
    14641 tggtgacttt gttttgatct atcaaaatca aacaacgcct gccaaaaatc tgcttttccc
    14701 acagtcgctg cgccatttga aagagcaaca aaaaaaataa tcaaaagaac aaaacagacc
    14761 aaaagcagga gtctattttt cttcatagtc ctcataattg atcctcctgc tcttttggca
    14821 acaaatcata ggttagaaaa actggtttat ttgttcttgg aagcgtgaca atcgttgcat
    14881 caatctcaaa tatttttttc atacactctt ttgtgatcac ttcttcaggt gtaccttctg
    14941 tgatcaactt accgtttttc attgctaata aataatctga aaaacgtgaa gcatgattaa
    15001 tgtcatgaat cgacatcaag atcgttgttt gtttcatttg attgatttca gtcaataact
    15061 gtaggatctc taattggtgt gccggatcta aaaatgttgt cggttcatcc aagatcaaga
    15121 tatctgtttc ctgagcaagc gccatagcga tccagacacg ctgggtttgt ccaccagaca
    15181 gcgttgcgat atcccgcata cgaaatccag ctagacctgt agcatccaac gcccactcaa
    15241 ttgcttcatg atcttgcgtg tccatgccac tgaacagttt ttgataaggg tatcgtccgt
    15301 aagatactac ttcttctact gtcatacctg tagggtgttc actagtttgt gctaataatg
    15361 caattttttt tgcaagttct tttgaactgt atgtagagat atcagagtta ttgacaatga
    15421 tctttcctga tttcacaggt agtatacggg taaaactttt caataaagta gattttccac
    15481 ttccatttgg cccaatcaaa ctagtgattt tcccttcagg aatcgtaaca gacatgtttt
    15541 caatgatttt tttgttttcg tagccggctg caatgtcagc tgctcttaat ctcgtcattt
    15601 cacccatcct ttcgctgaaa atcattctca attatgacgc tgtttcaaca tttcgtcaag
    15661 cgctactttc atatctatag ccaaatgaag cggttgacag cgttcgaaat atcaggaata
    15721 ataaccaatg agaaagattc tcaattatgt taggaggaaa gaaatgaaat taaaaacaaa
    15781 agtattcaca ttatctagca tattgctgct ggctggagta ctgagcgctt gctccccctc
    15841 ttcagataaa gaaacagcta caactgccaa ttcgacgact tcatccaata gtactcatac
    15901 agtcacagat acacttggac ataaagtaga gattccaaac gcgcccaaac ggatcatcgg
    15961 cagttactta gaagactatc tgattgcact aggggaaaaa cctatcgccc agtggactgt
    16021 tggcagtggt tccattcaag attatctgca agatgattta aaggatgtcc caacgatttc
    16081 ttatgacctt ccttatgaga aagtgttaag ctttgagcct gatttgctgt tgatttcttc
    16141 ttccgctact gtcgaaggcg ggaaatacga acaatatagc aaaatcgcgc caacttatgt
    16201 tgtaaaaaac ggaacagatg tcacatggga agaacaacta aaagatgtcg gcaaagcttt
    16261 ggcaaaagaa aaagaggcag aaaaaatcat ttccgattat caaaaatcca tcaagaaaac
    16321 tcgagaagaa ctagcagata aaatcgagaa caagacagct gcagtgttgt gggtgaccaa
    16381 caactcagcc tttatggtca gaaaaaatcg gtcaagtgga agaatcgtat acgacgatct
    16441 ggaatttggt gttcctgatc tagtggaaga ggtctccaaa aaagccactt ctgactggtc
    16501 tgctgtttcc tctgaaaagc tagctgaatt agatgctgac tacatcatcc ttgtgaacag
    16561 tgacaaagat gctgccatgt tcaatgaacc aagctggaag aatctaaaag cagtgacaga
    16621 caatcatgta ctgcaattcg gacctgaatc tagttggcta tataacggac ctattgccag
    16681 tcaggaaatg gtcaatgata tcaaagaaaa actaaattga ttttaactaa aaaaagcatc
    16741 ttcacgatta gtcgtgagat gctttttttc ttattaacaa actagacgca tagattttat
    16801 tcttcttttg aagcaaccct attttttact ctttcccatt tttgaaaacg atgggcatat
    16861 agattttctt gatccgttat cccctcatac tcctctataa gggcgaagtc tgaaaaatca
    16921 atattcccaa tatacgtatc gccttcaaac gtgctatcga tcaatgttcg ccagatccga
    16981 ccagtgtaag gaagaagttc ttgaaaaatc cgactaccac ccgaaacata gatatcttct
    17041 gagatcgttt gactatacga caaaagttca tcgatcgaat gcattacttc cgcattttct
    17101 ttcgtgcgaa aatttggttg cgttgtcaac acgatgatat ggcgataagg aagtgaaaga
    17161 tcccccattc cttcataggt ctttcttccc atcactaaaa ttcgatccat tgtgtgttcg
    17221 cgaaagaaac gcatatcatt tggcaatcgc catggtaaca aaccattttt gccgatcaga
    17281 ccatttttat cttgtgccca catggaaata aacatattat ccgctcctta tgattttaga
    17341 tgttttgaga taaaaaaagt ctcaattctt tgaccagcaa ggaattgaga actttaaaaa
    17401 cattattttg tttcacggtg caaagtaact ttgcgttcgc gtgggcaata tttttgtttt
    17461 tccaaacgat caggattgtt acgtttattt ttgcttgtta gatagttacg ttctttacaa
    17521 gaagtacact ctaaagtgat gtttacgcgc atgtttttcc ctcccatttt tacaagaatc
    17581 ctctaagatg actctcagac ttgattatct taccatgatt ttttctagat tgctagtgtt
    17641 tttcacgatt ttgttaagta aaactacttg acgataaata caacggtttt acttctcaaa
    17701 caaaatcttt ttctttttgt ataaataccc ttacacttgt ttatatagat tatgtttttt
    17761 aataaagggg gatactaata tgccatggaa catgaaggac tttcctgcat caatgaaaaa
    17821 cttagataaa ttgacaagaa aaaaagcaat cgacatcgca aatgcattat tagatgaagg
    17881 gtaccctgat aaccgtgcga tcccaattgc cattgatcaa gcaaaaaaat ggtatgaaga
    17941 tgctagtgaa tcagaaaaac gaactttcga aaaagaaaaa aatccaagca agacggatga
    18001 acacgatacg aatcctcgtg ctggaaaact gcttgattca gatgtgatcg tagaatatga
    18061 ggaagaacaa tggatcgtga aatctaaagg ggcaaaaaag gcaagcaacc attttgatac
    18121 aaaaaaagaa gcaatcgaaa aaggaaagca agtagcacaa aacaaagaat caactttgtt
    18181 catttataaa aaagatggga caaaagaaaa agaaatcgat tattgaacat atgtaaaggg
    18241 gttgtgacga aaatcgtgtc acaaccccct tatgttcgaa acaatggtgt tcctctgcca
    18301 actccgaacc ataagtcata atatctataa aacgggaaga gctgtttttt gaaattgttt
    18361 cttatcactc agtgttaaac ggtaggagaa aacctctttt ttattgatat ttgattttca
    18421 tcaaacagtc acatagtcga ttgcttctgc ttgcggaatc gtttcatcta caaagtagac
    18481 tttataccat gtcaagaaac tgaagattgt ccagattttt ctttgctctt ctttacgtcc
    18541 ttcgtgatgg tcatctagca acttcatgat tttttcttga tcaaaaaatt cttttgcaaa
    18601 atctgcttcg aataatgctt tgacttgttc gtagccgtgt tcttctttca accaagcttt
    18661 gattggcaca gggaatccta atttcacacg attcgaccat tcttccggaa gatgtttgtt
    18721 cgcagcttgt ctgaaaatat cttttgtgtt gttttgattc aataaaaact tacttggtat
    18781 ttgttgagcc aatttcatga cttcgatatc caataaaggt actcggactt ccaaagaact
    18841 tgccatagaa agtttatctg cctttaacaa gatatctttt ggcatccatt gatgaagatc
    18901 gacatattgc atcttattga cttcatcttt gattccttct gttttctcgt aatgaacagt
    18961 catgatttca tccacagatg gtgctgtttg atattttggt gttaacactt ctgatgcttc
    19021 tgattcttcg aacactcgag catgaccgat aaaatattct tttgcaggag caagtgattc
    19081 atataaatgg atccgtccat ggaaattttt catttttccg attttacgtc ctaaattata
    19141 gcgtgttcct tttggtaatt ttttcaatcc ttgagcaatc acacggatga attttgaatt
    19201 cgtatggaat ccatacgttt ggtatcccgc aaacagttca tccgcccctt ctccagattg
    19261 gactacccgc acactttgtg aagcaagatt tgctaaaaag tacaaaggta cgcaagaagg
    19321 atttgaatca ggttcatcta agtgatactg gatcaaaggg aatgctttga acgacatgtc
    19381 tccgtcgatc actgctgctg tattatctaa gtccaatagt tcggttagtt ttctggcttc
    19441 aatcgcttca ttataggttt tatcatcaaa cccgattgag aaagaatgat ctggtttcaa
    19501 tacagaggtt acataactgg aatcaacccc gctggataag aaagaaccaa cttctacatc
    19561 actgaccgta tgtgcttcaa tagaagcttg aacggtttcg tcgatttttt cgatccattc
    19621 ttgtcgggaa tgcgtttctt ttgtttcaaa atctgcatcc caatattgct gaatgtctat
    19681 ttttccatct ttatacgtat aataatggcc ttcaggcaaa cgatagacat ctttgaagaa
    19741 tgtttcgccg tttaatggag aatattggaa agtcatataa ggtttcaacg cttctttgtt
    19801 caattctttg ttaaaatcag gatgaggcag aaaacttttg atttctgaac caaacataaa
    19861 tgttccgttc atttctgcat aataatatgg tttgatccca aagtggtctc ttgcaccgaa
    19921 taactcattc ttttcattat cccaaatagc aaaagcaaac attccgcgga tcttttgcaa
    19981 taattctgtt ccccattctt cgtaaccatg caaaaggact tctgtatctg catgtgtttg
    20041 aaaaacgtgg ccagctgcaa tcaattcttc tctcaaaggc tggtaattat agatttcgcc
    20101 attaaaaata atgatctttg tccgatcttc attgtaaata ggctgagtac cgccctctaa
    20161 gtcaataata cttaatcttc tgaatcctaa agcaacatgt ttatcgatat attctcctga
    20221 actattaggt ccacggtgaa cgattcgatc catcattgta ttaataattg tttttttgtt
    20281 gtccttgtcg ttaacaaaac ctacaatacc gcacatttaa ctttcacatc ctaatataaa
    20341 attactgttt atttcattca tatgcatctt atcatagaat aaatagcgga cgaatatttt
    20401 ctctcttaca atttcgtcac gtgaaaacaa aagatgaaag tttctgtttt tacagaaaat
    20461 aaagtcccaa tatctaattt accatgtttt cttttttgag gctaccttat tctcttcgtg
    20521 ttaaaaaaat attagtggat gctatttcca aaaaaaccag caaaaaaaga cacgacctaa
    20581 agatcgtgtc taattatgac tgtatcagaa aatatgatac caaacggatc tattcattaa
    20641 ttaattgaca tagtttatct gttgctcttt tatgactgga taactgacca attcatgacg
    20701 atccaagtct tctttattca tatttacagc agtaatttga ctgtcttcat acgtacgata
    20761 ataataaatc cctttatcaa cgttacagca agaagaataa atcgtatatt cgtattttct
    20821 ttctccagta tcgcatactc ctttttgttg ctcgactgag cctagtatat gaaaaaattg
    20881 actgatactt tcagattctg aatcacctga tgcagcattc attttagtaa atgtggcttt
    20941 tacaaatcga gaaacagaag agagatctcc aggtaagccc aatcctccca tcccacgact
    21001 ataaacattc aagctgatct ggtttgaaaa atgattttct ggtgttccat ttgatagcga
    21061 gcgataattg ttcagattaa ataattgata atcaaatggc ggattatttg tcaacacacc
    21121 gacaggatta tcatataaat gaagtccatc tttcatactt tcaattacta tcgatttttc
    21181 tttatcagct aataaccagt gtaaaggtga caagggtagt ttttcactaa aatcgatatt
    21241 gactagattg atagtagcca ataattcttt agcttcgtct atggttgagc attgccctag
    21301 tatccatgga ataaactcaa atggtgcaac gttatctttt ccttcttgta attctttata
    21361 atcagcattg ccaggaaaat tcagtcctgc catactcaac cctttttcat ttgtagcctc
    21421 ataatagagt ggatagttgg ataccccagc tgctattcct atcattgcat aatgtttatc
    21481 taaatttttt acctttctaa aatcgaaacg ataatttctt ggagtaatag tgaccacttc
    21541 attataagac atctcataat caaaattcct tccaaaataa tggtctttcg ttgcataagt
    21601 gattgcggta cacatcgttt cccgtcctaa aacaaatcgt ttttacactt ttatcttaca
    21661 tctagtattt tttttatcaa aaaaaggcca aagaagattg atttactagc atttataaaa
    21721 aatagtactc agggggaatc aaaaaatatt gtaggttaaa agtacaacaa taaggggctg
    21781 tgacaaaagt cttgtcacag ccccttattc cgaacaaacg gtgttcaaaa gcttgcatct
    21841 gcggtaataa gcccaatcaa acgaaaaaat aggaaaagct atttttgttt ggccgttctt
    21901 attacttgat gcaggacagc ttcttcacaa cctctaatcg tt
//
LOCUS       DAFEPQ010000042        21797 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.042, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000042
VERSION     DAFEPQ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.042"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(392..1459)
                     /locus_tag="LP543_001724"
     CDS             complement(392..1459)
                     /locus_tag="LP543_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL2321891.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(1479..1958)
                     /locus_tag="LP543_001725"
     CDS             complement(1479..1958)
                     /locus_tag="LP543_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HBL2321892.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(1975..2436)
                     /locus_tag="LP543_001726"
     CDS             complement(1975..2436)
                     /locus_tag="LP543_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL2321893.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(2450..3700)
                     /locus_tag="LP543_001727"
     CDS             complement(2450..3700)
                     /locus_tag="LP543_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBL2321894.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(4078..5529)
                     /locus_tag="LP543_001728"
     CDS             complement(4078..5529)
                     /locus_tag="LP543_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBL2321895.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(5850..6614)
                     /locus_tag="LP543_001729"
     CDS             complement(5850..6614)
                     /locus_tag="LP543_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBL2321896.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(6642..7340)
                     /locus_tag="LP543_001730"
     CDS             complement(6642..7340)
                     /locus_tag="LP543_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBL2321897.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(7352..8131)
                     /locus_tag="LP543_001731"
     CDS             complement(7352..8131)
                     /locus_tag="LP543_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBL2321898.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(8147..9091)
                     /locus_tag="LP543_001732"
     CDS             complement(8147..9091)
                     /locus_tag="LP543_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL2321899.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(9137..9916)
                     /locus_tag="LP543_001733"
     CDS             complement(9137..9916)
                     /locus_tag="LP543_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321900.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(10296..12371)
                     /locus_tag="LP543_001734"
     CDS             complement(10296..12371)
                     /locus_tag="LP543_001734"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBL2321901.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(12373..13290)
                     /gene="glyQ"
                     /locus_tag="LP543_001735"
     CDS             complement(12373..13290)
                     /gene="glyQ"
                     /locus_tag="LP543_001735"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBL2321902.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(13676..14494)
                     /gene="recO"
                     /locus_tag="LP543_001736"
     CDS             complement(13676..14494)
                     /gene="recO"
                     /locus_tag="LP543_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBL2321903.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(14637..15536)
                     /gene="era"
                     /locus_tag="LP543_001737"
     CDS             complement(14637..15536)
                     /gene="era"
                     /locus_tag="LP543_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBL2321904.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(15551..15946)
                     /locus_tag="LP543_001738"
     CDS             complement(15551..15946)
                     /locus_tag="LP543_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBL2321905.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(15930..16406)
                     /gene="ybeY"
                     /locus_tag="LP543_001739"
     CDS             complement(15930..16406)
                     /gene="ybeY"
                     /locus_tag="LP543_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBL2321906.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(16435..18627)
                     /locus_tag="LP543_001740"
     CDS             complement(16435..18627)
                     /locus_tag="LP543_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBL2321907.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(18651..19622)
                     /locus_tag="LP543_001741"
     CDS             complement(18651..19622)
                     /locus_tag="LP543_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBL2321908.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            20327..21706
                     /gene="brnQ"
                     /locus_tag="LP543_001742"
     CDS             20327..21706
                     /gene="brnQ"
                     /locus_tag="LP543_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBL2321909.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
BASE COUNT         6106 a         4590 c         3659 g         7442 t
ORIGIN      
        1 tatgctttcc cttggaataa tgaaaatata cttttgtatt acacaatatt gcaattttat
       61 ttcttaatat gattatttca ctcattcatt actgccacat atacacaact tataaccacc
      121 ccctgcaaag tttagggtaa gttatcagat aatatcttcc tcttcatcat ccagaataca
      181 aaatctattt atttatagaa atatcattaa tgtttttata taatgtaccg tgcttgtact
      241 cttgctctat tacaagcact aaaacactac tattcatttg aattcttaca cggataaaaa
      301 tttacatcgt ctatttcaat attttttctc tctattttat tcacttaata agttcattac
      361 ataatcgaaa cattttttat tttccttgtc atcactcctt gctccctttt tgatacagat
      421 ttaaaattct atcaaagaaa tttccatcgt taaatttcgt tattgccaaa tccttcgaac
      481 aacgtgaaaa ttttacgtat ttatctttat cattgatttt tagtattgca gatgtcaacg
      541 ctgatacgtc accaggttga aacaaatatc cattttctcc atcacgtact gctgaagaaa
      601 tatctcctac atctgtagca attacaggca ttccataaga aatcgcctct agaatagata
      661 taggcaatcc ctcgttatag gacggtaaaa tcatcacatt tgatcttcta tagcactcct
      721 cctttttttc tcccacaacc caacctgtaa attcaaaata cgattgcaaa ttatattctt
      781 ctatcaattc tttgagcgat atttcttcat cacctgctcc agcaatctca aaaataaact
      841 ttttttctgg atattgttct gcaatgtttt taactgcttc taccaaatcg tgtacacctt
      901 ttcgctttat caaaactccc ataaacagaa atcggcacat ttttgaattc cactttatgg
      961 tatattctgg aactacaata gaattttcta caacaacagt tttacaattt ggctcaattt
     1021 ctttaattat cttcttccat ttttctccaa gtacaatcgt ggaatcagac tctcttaaca
     1081 gcaagcgaat ctgattttgt ttttttacag aaacactatc ataccatttt ttaaattccg
     1141 atccatgaag atggatgatt acaggaatat ttaataatct aaaccactta ttcagcacat
     1201 atttcctact aaaactccct ctgtacgaca tgtgcatatg aataatatta ggtttactca
     1261 ataaagagat acctataatt ttagcaaatg catacataaa atataaagat ttaaatattg
     1321 catttccacc cttataactc ggtataaaag tcatctcgat attctttttt tcccaattaa
     1381 aattgagcat ttgtgtaatt atacttgtaa ttccaccttt taccgagctg tcattaccaa
     1441 tcataagtac tctttgcata acgctttact ccttatattt atattttttc tattaatacc
     1501 tctatcgaat taataatcgt ttccgtgtta gatacatact tgttaaacat ccgaactctt
     1561 gtttctttaa cggcatcttc taaattttct aattcataac aaggatagat aatattcatc
     1621 tcatcaaatt gtctaattaa ctgcagttgg tgatcatcga catgctcacc atacttagcc
     1681 aatcgaggaa cagcaatgac ccttttacct ttttttacgg ccccaattat agcacctgta
     1741 cctccatgag taatcaccat ttctgctttt tgcatagttt cagtaaactc atccctattt
     1801 agaaatgctt tataggaata atttagtggt ttataatcac tataaccact ctgtgcaaat
     1861 gtactttcga ttattactcc tttttcaatt aaatcatcaa tagcttttaa caatctatca
     1921 aattgaaatt tttgtgaacc taatgttata aatatcattt ttttctccta ttatttaata
     1981 tatcccaccc aaataaatag cattaggata aaattgtagc atagtttccc actgaacata
     2041 aaattgatct gcaaaatgat acatcaattt tcctgtctga gtagggctgg ttacttttgc
     2101 aaacgattca atataaatca gtttttttcc catcattttt gttattaagc agatagggat
     2161 catagctaaa acacctgtac aaatcacgag atccggtttt tcctttatat atataataat
     2221 tgatttgaat aaattaataa gtatagtaca caaaaaaaac ttttcttttc tatttacttg
     2281 tctcatgtag tacgtttttt cgccttttga ctcagctgaa tattctgtct gttctgttat
     2341 aacaaaactc tcatacttct ccatcagtgg ttttaacatc attaactgtt cataatgccc
     2401 cccagaggaa gccgcaaaac atatcttttt gttcatatta acctctctat cagtcacgct
     2461 gaaaaatatc tctagtgtaa actttttctt taatatcatc caagcaggta tcatatcgat
     2521 tggcaataat agaatcacac atcgccttaa actgctctaa atcgttgata accttacttc
     2581 cgaaaaaagt ctctccatct ttcagtgcag gctcataaat caccacagtc gcacctttag
     2641 cttttacgcg cttcataact ccctggattg aactctgacg aaaattatca ctgttgctct
     2701 tcatggtcag ccggtatata ccgattaaaa ctggattttc cctgttgaca tcatactgat
     2761 tatattcagc gtagttgtaa tagcccgcca tctgaagaac acgatctgcg atgaaatcct
     2821 tacgggtacg attagattca acgatagcct caatcaaatt tccaggaaca tctgcatagt
     2881 ttgccaacaa ctgcttggta tcttttggca agcaataacc tccatatcca aaggatggat
     2941 tgttgtagtg agttccaata cgaggatcga gacaaacacc attgattatc tgctgagtat
     3001 tcagcccctt catttcagca taagtgtcta attcattgaa atagctgact cgcagtgcta
     3061 aataggtatt tgcaaacagc tttactgctt cggcttcagt gaatcccata aaaagagtat
     3121 ccacgttctc cttaattgca cttttctgca gaagtgttgc aaaaacatga gcagcattta
     3181 acaagcgctc atcagtcata tcagtaccaa caataatacg agatggatac agattatcat
     3241 ataaagcctt gctctcgcgc agaaactctg gactaaaaat aatgttctta ctccccgttg
     3301 tctcacggac tgattcagtg tagccaacag gaattgtaga cttgatgacc atgatggtat
     3361 caggattcac gtccatcaca acctcaataa ctgactcaac agcagaagta tcgaaatgct
     3421 gagtacgact gtcatagtta gtcggtgtag caataacaac gaaatctgcg gatttataag
     3481 cgctccttgc atccaaagta gcagttaagt caagttcttt ttctgtaaga tacttttcaa
     3541 tatattcatc ctgaattgga gacttccgtt tattaatcag ttcaatcttt tcagggataa
     3601 tgtctaccgc ggttacctgg tgatgctgcg ccaatagtgt ggcgatactc aaaccaacat
     3661 aaccggtgcc ggctactgcg atgttgtact ttttcttcac aatatctcct ctttcctctc
     3721 ttaagtatgg agaatgttac tcctacggct attcatcatg tcaaaataac acagcttaga
     3781 atgttgataa aataattttc cagaatgtga taaaatctct ttcatttata aaccacacat
     3841 ctaattttat ctattctcca caagacatgt agagtattct ttaataacaa gatacgacct
     3901 tactaaatac ttcaaaaaat tatcatattt aattcataaa tataaatctt cattttaaat
     3961 gtatgcccaa aaatttatct gtaatgcgtc atcaaaatct agattccaaa tctttatcaa
     4021 tttttatcca ctcattctac cactttagta atattttcta tttataacac tttcgtccta
     4081 cattgatccc cccctctgaa gtacagtttt tactgtctta aataaaattt taaaatctaa
     4141 ccccatactc caatttttta tatattccgt gtcaagtttt gctacttctt caaaatcagt
     4201 aatatcactt ctaccactta cctgccacat tccagtaatt cctggcttga tagcaattct
     4261 tgcacgatga tgataatcat attcttcata ctctgattta aggcatggtc gaaatcctat
     4321 aaggctcatc tttcctgtta gaacgttgaa aaattgaggg aattcatcta aagacgtgga
     4381 tctaataaat tgaccgattc ctgtcttttt tgttccatct ggaagtattt tatttccgat
     4441 aattcttgga tcaaactcca tcttaaacat aagattattg ctcatcttat tttctttcat
     4501 taattctact tttctttctt ctgcatccat atacatactt ctaaacttat acatcttgaa
     4561 cggtttacca ttctgaccta tacgttcttg tgcaaagaaa attgggcctg gtgaattaat
     4621 ataaataagt ggagcaataa agataaaaat aattcccgtt gcaatacatc caacaatacc
     4681 tgcaagaata tcaattgttc gttttataaa tgcctgttga gttgtcataa cattgatact
     4741 tgttgtaagt actgtatatt ctcctatacg accaatagat tgttttccca aagcactatc
     4801 tgatactttt gcaagattca aatgtacagt aaggcccatt tcagagcatc gttcaataaa
     4861 ttctttcata cacgaagtac ttttattcaa aataataaag acttcatcta cccatccctg
     4921 acataagtaa tccgctgcat ttttggaatc agaaacaact gtcactccct caacatcttc
     4981 accaatcatg cttttatcta tgataatgac accatttatt ttatacattt tatagttatg
     5041 ctctttcaca ttacgaataa ctacttcagc aatactacta gatgtaacta taataagtga
     5101 tttgttcccc tcatttttca ttttatgttt caagtaatgt ttccataaga ggcgagttat
     5161 atatgtaatt acaacgtata aacctcccat caagaaaaga acgactctag aataatcatt
     5221 tccaccctga acactaatga gatacaaact tcctaacagc accaacataa tcgcttgctt
     5281 tacagaagaa gcaaattcta tatagtaccc tctttttaat acacctctat atgcttcaaa
     5341 catgaaaata actaaaagat ctgccagttc aatgaagact gccatgtttt gatacattgg
     5401 aatcacataa ggattccaat ctacctggcg taagacatac gatagataaa aagcaagctg
     5461 gatacacaat aaatctaaaa taataaaatc aatatgttta acccatcccc ttgaactttt
     5521 cttgtacatt cctttttact cccctctttt taataaaata aattgtatta cttgttttct
     5581 ctcctaaata tctttctgaa ggattattga ctcgacaaat tggcttgtgg atattcttat
     5641 atatagaaca tccgtaagcc aacttaaaac tccttaacaa aattttttta ctcatattgt
     5701 agatcaaaac aagtatccaa gaaatcatat tttatttctt ttttctgctt actcaacaac
     5761 agctaaaaga aaaatcatca taaacaaatt caactaagcc aacgatcccc ttgtgaaatc
     5821 aagttttttc ttgatttacg aataaagagc taaaataaac caaacttctt cttttttacc
     5881 tcttcataga ctggataact gatttcatcc ccgttgatca gatcctttgc gacttgtttc
     5941 atctgtacta ctttttcctt accaaagtcc tttgcaatct gctcatagca ctcttttaag
     6001 taaaaggtgc gcttagagac cccatgggca tcagaagcag ccatctgcac gagattatgt
     6061 ttgaccatcg ttttggctgt tttctggatg tctttgccaa ataccccaac ataacttggc
     6121 gcagtgagtt gggccaaaca tcccatatct aggaacggaa gcaaatgatt tgggtcttcg
     6181 cgaaaatgca cgttgcgttc tggatggacg attactggtg tctttcctag ttctaacaac
     6241 tcaaaaaaaa gccgttctgc atataacggc acttccagtg tcggaaattc gatcagaaga
     6301 tatgtatcat ctaaatctgt aaataatatt tcatctcggt ggatgtcttc gatgagcgtt
     6361 ccggtaatac gtacttcttg tccttccaat aacgtcaaag gcaactgtct tttctctaat
     6421 tcggcttgta acgaagcgac tagagaaatg acttgtgatt tttcattgct gtattttcca
     6481 ttattgtggt gtggtgtgca taaaatatgc gtgatgcctt gctgaatcgc tttttcagcc
     6541 attgcaatac ttgcttctaa gttttctgcc ccgtcatcga ttcctggtag gatatggcaa
     6601 tgtagatcga tcatttctct tgtctcctct cttcttattc cttaatttcc gtagtaataa
     6661 ccagcatctt tgctatgttc tgctccgttg tacacaacac ctaagactcg tgcttgtacc
     6721 atctccagca gttcttttgc tttagtcaag gattctttac gtgacatatt ttcacgtacg
     6781 accaataaag tgccatccgt ttttaaagac atgatctgtg catctgttac ggctactact
     6841 ggtggcatat cgaatatcac cacgtcaaat aactgtcttg cttcggatag gatctgatcc
     6901 attcttggcg agcttaacaa ttctgatggg ttgggtggct taggtccact aggcaaaatc
     6961 gacaagttct cgactggcgt tcgttggatc acatctgcta cacttcctga tgaactaagt
     7021 gctgtactta atccactggc attgttcagc tggaatgtct tatagacgac gggtttacgt
     7081 aaatctgcat cgactagtag aactcgttga cctgatttag caaaaacgac tgcaatgttt
     7141 gctgcagtgg tggatttccc ttctccagga cctgatgaag tcacgacaat cgttttgatt
     7201 tgctgaccag ctgcggaggc aaactggata ttcgtccgga tcgtacgata ttgttcggcg
     7261 attggtgaag atgggtttgt taatgtgatc aaactgactg catgagtttg tgctgctttt
     7321 tgtttctgtg ttcgtgccat ttttcttcct cctagactct tgagcggctt ctgcgtgatg
     7381 gggctgtttt atcgtttgta gcttttggtc gtgcaggagc tgttcctttt ttacggatac
     7441 cagcgttcaa ttctttcgct gacatttctg gaactgttcc taagattggg aatcctaatg
     7501 tatctgtcac atatttttca tctttcactg tccgatcgag taattccaat aagaatgcca
     7561 gacctacacc taccatcatt cctaacatta agccagtcat taaattcaat ttattatttg
     7621 gcgaaacagg tgtcgtattg gcaactgcac ctgaaatgat cgagatttta tcaacattca
     7681 tcacatcttt tgcgttctct tggaaaactt gtgaagttgt gttagcgatc tgttgagcag
     7741 ccactgcatt cgtacttatt gcctgaatcg agaacatttg tgaattttct gcctgattaa
     7801 ctgagatggc atctttgatt tgttcagcag tcattttcac attgtcttct gtttctaaac
     7861 gatctttgac ttcattgagc actaaatcac tgacgatcat atctttatac gtattgatca
     7921 tttgcaggtt cgtattgaca tcattgacat ttgcagtgtc tgattgtggc agtgtcacga
     7981 tcaattgtgc ttgtgaactg tattttggtg tcaagatgaa aaatgttacg attcctgaga
     8041 tggccaagcc agcaaacatt gagatgatga ttattaccaa gtgtttgcgt aatgtttcaa
     8101 aaatttcttt caagctgatt gtctcttcca ttttttctcc actttcctat ttattctcta
     8161 gattcatttg atcttttaat tcttttttca cacgatcaag ttcttgatca ctgacttttt
     8221 gataggagat attatcttgc atgaagcctt ctccttgcag ctgatcttgc ttaatcgtac
     8281 caaatgcctc ccgataatct aaagcaattt ttttcatttc atcaaagttc atgtctgttt
     8341 tcatattcga ctctagagca ttcaaaacgg attgatagtt gctgacaccg tttagactca
     8401 atactttttt cgctacgcct tctacgatct ttctttggcg cccttgtcta ccgtaatcac
     8461 catttgggtc ttcataacgc atacgagaat aagataatgc ctgatccccg ttcaatgtaa
     8521 ttttaccgat tggaaagtca tagccatctt gtgagaaagt gaggtcatta ttgacttcga
     8581 taccaccaac tgcatcaacg agttccttta gtcctttcat attgatagag acatagtgat
     8641 cgattgggat gtctacatat ttttgtacgg tatccatcgc catggcagct ccgccgaatg
     8701 cgtaagcgtg gttgatcttg tcttttgtgt cacgacctac gatgtcgaca tacgtatctc
     8761 gaggaatgct gacgatcgtt gtctgtttgt cttcaggact gactgtggca accatcatcg
     8821 tatctgatct tccttgatcg acccgaccta aatctccagt atcaatcccc atcaatagaa
     8881 cagagaaaga ttcttgtttg tccagatcaa ctggtttcgt tcgttctcct tgggttcgtt
     8941 ccactgattc gtaagttttc tcaatagaat gtgagagatc cagatataat tttgctccta
     9001 ctcctacaat tgctaatact aatacgataa gaactgctaa aataatcaaa aatatttttt
     9061 tccctcttga ttttcttctt cgtcttgcca tagtttcctc cgtttgctaa ccttgcttga
     9121 atcaacgttt taactgtcaa tagcttatat tgctatagcc catttgctga gcaacagttt
     9181 gtgcttttgt tgcgtatgcg gctcttattt tttcattccg gatctgctct atttgactga
     9241 gcgccgtcaa atattgatcg taagtagcgg tagctgttac ttgaccgtct ttcagcatcg
     9301 tatcgaatag ctgatctagt tgcgtgactt gtgcaatttg ggtatcagcg aatcctagat
     9361 attgcataac gactgttttc caagcactat ccttaaaaaa gctcatattt tcacgtacat
     9421 gggcgacatc tgcacttgcc aacttttctt tgatgatgac atcatccaca gcttgctgcc
     9481 agtttgatac gtttttagta aataatttat tgacagccgt ctggatcttc agttttgatt
     9541 ctgcatcctc taactgcgtt aagactgctt tcttttcttc ttggatcgca gcagcttctt
     9601 taggtaattc tgattccttg atgtcataat catctgccga aagtttgata gaagatagtt
     9661 tatgtgacaa atttgaaact gtttcttctg ttatttcagg atttaggaaa attttttctt
     9721 ttgtactgta cagcttatct acttcttttg ataaagaggt cacggtgtct ttttgattgg
     9781 tgatggcaga ttctgcgtaa ttgatccgtt gatgatattg ccgctgatta ttgagatggt
     9841 atccaaacaa gcaaatcaca atagcgcctg tgcctaccgc cacagtagcc aataacttga
     9901 cgaacggctt ttccatagat tcactcctaa atattgatga tagataagta aagatgtgat
     9961 tcattcacat cagaatgagc gctttttatt ttaaaagcat aattaaacta atatattcat
    10021 agaaatattt ataaacaggt tttccctata taatcatacc acatacagtt tttttgacta
    10081 gggaaattcc taatttaaaa gtaaataaaa cagtaacaag attataatta atcgttttca
    10141 tttttatttc ctcacttttc atgagcgttt tgaaaattcg aacaaagaat aaaataacaa
    10201 aaattattaa aatagaatta ataacagcta tttataacaa aaaaaacaga gacacagcac
    10261 gtttcgtgca tgctctctgt ttttcaaaca aaagactatt ttgtgatgat ttgatccaag
    10321 cttgctaatg acaaagccat ggcagctatt tgacttagtt gtgttagtcg gttcttacgt
    10381 actgctggtt catcagccat gaccatcgtt tgatcaaaat aattttcgat cagtggacga
    10441 agagcgacaa gctggtcgta gttttcgtgg atcgtttggt cagcaaaact tgctttcatt
    10501 gcttctactg ctttgtgtaa ttctttctct gcgtcgtttt cgaacagctc agggtctact
    10561 tctgattgtt tttcaagctg cgcttttttc gctaagttca cgacacgtgt caaagcttcc
    10621 attgaaggtt tgaagtcttc atctgcaaga tgttgtttga acaattgtgc tgctgcaaat
    10681 acttttgtca gatctttttg ttccgcattc aaaacagcat cgatgacatc atgacgaata
    10741 ttcttcgttg tcaataattg gcgcagacgg cctttgataa agtcgatcac ttgttgttgc
    10801 ccttctgata aaccaatgcc gaaacgatcc gtatctttac tgataacatc gctaatcgtt
    10861 tcttgcagaa cagacaaagg aaaggcccat tttttcgatt cgatgatccg aacgacaccg
    10921 tatgcttggc gacgtaaggc ataaggatcg tttgacccgc ttggaatcat cccaactgca
    10981 aagaaagaaa agatgctatc taatttatct gcaatcgcta aaacagaacc tactgtagac
    11041 tgaggtagct ctccttcact ggatataggc atgtaatgct cacgaatcgc tgtcgctaca
    11101 gcaggtttct cgcctttcag aagagcatat ttttcgccca tgatcccttg tagttcagga
    11161 aattccccta ccatgttggt tactagatca aatttataga tttcagcagc tcgttgcaaa
    11221 tccatcaatt cttcctcaga tagtcctact tgttcgccaa tgatacgggc aatgacttgt
    11281 acccgttgca ttttctcata gatactaccg attttttcat ggaaagtaac agtttttagt
    11341 ttttcaacgt attggtcgat tgaagctttc ttgtcttcat tataaaagaa ttccgcatct
    11401 tctaaacgag ccgtcaatac tttttgattg cctcgaacca catcttccaa ttttacccga
    11461 tcaccattac gagcagaaat aaaatgagga agcagcatac cttgatcatt ccgtacctcg
    11521 aaataacgtt gatgttcttt catagaagtg actagtactt cttcaggaac agaaagatat
    11581 ttttcttcga agtcgccaga aaagacagtt ggatattcga ctaaattgtt gacttcttct
    11641 agtaaatctt cgtctagatc aataacccag tgattttttt ctgctaattc gtttgcttgt
    11701 gtaacgatca tttcttgtct tttttgcgga gacgcaatga cgaattgttc attcaatttt
    11761 tcttcatatt cttttgcatg cgcaaatgtc acatcttcgc ctaagaaacg atgacctcga
    11821 gaggctctgc ctgttttgac atccaagatt tcgaatggga ttacttcgtc atctaacaat
    11881 gccacgatcc agtgaatagg gcggatatat tcaaaatcgt attctgccca atgcatcgtt
    11941 acagggaaag tcaaactttt gatcacccct gctaagcctg tcaatacttc ttttgcttcc
    12001 ttaccatgag tgtatttcgt tacatagaca tattccacac cttttaattc tctaaaagtg
    12061 atagcgtctg tcgtcagacc ttgtccgcgg acaaatcctt ccgctgcttt cgtccagttt
    12121 ccatctgcgt ccaaagcaat ttttttggct ggtcctttga cttcttcttc actgtccgct
    12181 tgtcgttctg gaatatttgt tactttgatt gctaaacggc gcggtgtgga aaatgtttcg
    12241 atcgtatcat aagtcaattg gttttcatct aaaaattttg ctgttttttc ttctaattgt
    12301 ttcatgcttg gtgtgacaac atgggcaggc atttcttcca gcccgatttc aagtaataag
    12361 tcttttgcca tcttattcag cctccttcac ggtttctgga tttgctgtgt ttttattcaa
    12421 caaagggaat cctaattttt cgcgttctgc cacaaagatt ttggctacag cacgagccat
    12481 gttgcggatg cgagaaagat aaccggcacg ctctgttacg gatactgcac cgcgggcgtc
    12541 taataagttg aatgtatggc tacatttcaa gatataatca tacgccggat gaacaagatt
    12601 ttcatcgata cagcgtttag cttctttttc aaaacgatca aagttgctta gtagcaagtc
    12661 ttggtcgctt acttcaaaag aatatttgga atgttcatat tctggttgtt tgaagatctc
    12721 gccatatttc acgccatcag cccattctaa atcatagaca ctttcaactt cttggatata
    12781 agaagcaaga cgttctaatc cataagtgat ctcagaagtt actggtttac aaggcagtcc
    12841 accaacttgc tggaagtacg tgaattgtgt gatttccatt ccgtcaagcc atacttccca
    12901 acccacaccg gcacagccca tggatgggtt ctcccagttg tcttcaacaa aacggatgtc
    12961 gtgttctaat ggatcgattc ctaaaaggcg caagctttct aaataaagct cttgaatatt
    13021 ctcaggtgaa ggtttcatca ccacttggaa ttgatgatgc tgatacaaac ggtttgggtt
    13081 ttcaccataa cgtccatcag ctggtcggcg agatggctct acgtaagcag cattccatgg
    13141 ttctggtccg attgcccgta aaaaggtata tgggctcatc gtacctgctc ctttttctgt
    13201 gtcataagct tgcatcagca tgcagccgtt tgatgaccaa aatttttgta atgttaaaat
    13261 catatcttgg acggtcaatt ttttactcat ctgttttgcc cctttcaatt ttaaagcgtt
    13321 gctgtcaaat aagaatacag gaaagaaaac gacaaaaaat ccctatgtct gattacagac
    13381 atagggacga ataattcgcg gttccaccct acttccgaat aaagattcgg cagcttcatt
    13441 tatcgtactc gcaagtgcct cttcaaaaat cgtttgggtc cggctcccac tctcccgaac
    13501 tcgctaaacc acgtgttttt tactgatctt ggtcaatgta cgtattcatt ttgcagtttt
    13561 cttgtttcta taatagatcc tatcactgaa cgatctattt aatctatggt taataataga
    13621 cggaactccc tgtgtttgtc aaatggtttt gattttttgt tgtatgtttt atttactatt
    13681 cttctttttc aggtgtctct ttttcttttt cagacatgtc tttctctgac gtctttaata
    13741 cgtgtgccca agacttcatt tgattgatga actttttgct tttcaaatgg ataccgacat
    13801 attcatcata cagctggtcg atgagttcac ggattgcttg tttggtttct tctttcagat
    13861 ggattccact aattttatca taagaaatag ctgaaaacat ccgaacgaaa taaacagctc
    13921 gaggatctgc atgataacgg tgatcgtcca tcgaccaatg tctttcgcat atcacaccac
    13981 catatttgga agaatagtca aattttcctt ttgtttcccc gcatacgacg cagtgtgtcc
    14041 agattggtgc aattccgaag cgttgcagta tttggacttc aaaaatattc gtgatgattt
    14101 ctgcatcgtc tccttggtcc atccgttgca gtgcttgttg cgtaaactgg aacaaatgcg
    14161 ggtcgtactg atgatcttcg attgccgcat ctactagatt caataaatat gtgccatagc
    14221 cgctcaaaaa gatgtcttct tggatgtgtc gaaacgcatg gacatctttg acactgctta
    14281 aaaatgacaa accttcactt cgaaaattcc cgatataaac tgcttcagta aaagggagca
    14341 aagccggagc taagggctga ttttgccgat ggatctcttt gatataaaac atcattttgc
    14401 cagaagattc tgtaaagatt ttgactaatt tatctttttc tttgtaatta cggctagata
    14461 aaatcaagcc ttttgattct gcaagctggc tcatattctc cctcctaaaa aataaatctg
    14521 gaaaagcaga ttctgccgat ccagatcatt tatgttgcga tcttccgcag cttttctcaa
    14581 cacgcggatt tttattcagt ttatttctat acctaattta caagaagctc agaatatcag
    14641 tattcgtctt ttcggtagcc gaagtcttgc agatagactt ttttgtctcg ccagtctttt
    14701 tgaactttga cccaaagttc tagaaagact ttgtctccta aaagattttc gatatctttt
    14761 cttgctttcg ttcctacttg cttcagcatt tttccgcctt tgccgataat gatccctttt
    14821 tggctatctc tttcgacaat aatcgtagcc tggatatgga ttttatcatt ctcgtttcgc
    14881 ttcatcgaat caacaacgac tgcgacagaa tgaggcactt cgtctcgtgt taaaaacaag
    14941 actttctcac ggatcaattc agaaacgatg aagtattcag gatgatcagt cacttgatca
    15001 tctggaaaat attgtgggcc ctctggcatt tcactgacta atacatccat caaacgttcg
    15061 acgttgtttc cttctgtagc agagattgga atcacttgtg caaaaggcat ctgcgttgaa
    15121 taatcttcga tgattcccaa caactcgtct ggatggattt tgtcgatttt attgataatc
    15181 agataaacag gagaatgcac gttttttaga cgttcaataa taaaatcgtc tccccgaccg
    15241 cgtttttgat cagcactaat cataaataag accgcgtcca cttctttcaa cgcactgtaa
    15301 gctgcttcta ccataaaatc tcctaaacga tgctttggtt tatggattcc tggtgtatca
    15361 ataaaaatca actgagcttc tggtgtagtg tatactcctt ggattttatt tctagttgtc
    15421 tgtgccttgt cactcatgat ggcaattttt tgacccacga ttcgattcaa taatgtggat
    15481 ttccctacat ttgggcggcc gacgattgcg acaaaccctg atttatgttc tggcatatat
    15541 gttctccttt ttaattaaac aacaaatgat atattttagg gacaaataaa atcaacccaa
    15601 cgatgacggt aaaacaagcg gtcactaaaa ctgctccagc tgccatatct ttgatttttt
    15661 tcccaatcgg atgaaaatga aaatccgtaa acatgtccac gacattttca aaaacagtat
    15721 tgatgatctc tactatccat acgagaaaaa cagctaaaag cagccataac cattctatcc
    15781 gatcaagttg gaaaatcacg cccataaaca aagcgaggat tccgaacgca acatgtttcc
    15841 tcatgttgcg ttcttcctca aaaacagttt tgactccttg gacggcaaat tccacggaca
    15901 tcaggaaatg tttgtttttc tctacgtttt tatcttttaa gtccataggc atctaagatc
    15961 tctttctgta aaccaaacat ttctttctca tcctctggtg tcatatggtc atacccgttg
    16021 atatgcaaga atccatgaac cgctaaaaag ccaagttccc gatcatacga atggccgtaa
    16081 gcttctgctt gctctgcagc acgttcagta gagatcatga tgtctcccag tacacgaggc
    16141 aatggattct ctccatcttc aaaaatcact gggatttcgt cttcgccctc ttcttccaag
    16201 gcaaaactga tgacgtctgt cggtgcatct tttccacggt aatcacgatt gattacttgg
    16261 attgctgcat tgtccatgaa agtaacagac atttctgttt cttctggcaa ctctaaatat
    16321 gatgctgcaa attgcagcaa gtcgtcgatt tctttcattt tctcttctga gacttggttt
    16381 gtttcatcca tgaaggtaat atccattata catcgcttct ttctattctt cttgctattc
    16441 ttcttgttct tcttttatct tctcaacttc cgatttcatt ttaggatatt tgatccgaga
    16501 atggaaggta ctgcttaaac cgctgatcaa tgatttttct actttacgga tttcttttaa
    16561 cgtcaatcca ctgtcgtcca attgtccatc ggaaatacgc tcttcaataa gattatggac
    16621 aaaactttcg attttttcgc tcgttggatg gtccattgcc cgtactgccg cttcacaact
    16681 gtcagctata ttgacgattc ctgcttcacg gctttgaggt tttggtccag gataacggaa
    16741 ttgctcttcc gtaacttccg ggttgcgttc ctttgcctta acgtagaaat aacgcatcag
    16801 cgtggttcca tgatgctgtc tacaaatatc gatcaccatt tgcggcatat tataatcttc
    16861 caatattttg gcaccatctg tcacatgtcc aaagataata tgtttactgt cttctggcaa
    16921 taagaaattg tgcggatttt ccgctcctga aggcaagttc tcaacgaaaa agctggcgtg
    16981 tttgatcttg ccgatatcat gataatagca agctactcga gttagtaaag aacgtccacc
    17041 gatttctgct acagcattag cacttagatt ggctaccatc atgctatggt gatacgtacc
    17101 tggtgcttct tccagcaact gtttcaataa aggatgattg ggattgctga gttcatttaa
    17161 tacgatcaca ctatcatcat tgaccatcaa ttcaatatag gggtgcagac ctatcgtcag
    17221 caagaatgaa aacagtgcac ctgtgaatcc gcagactagc gttaaccaag tcgttgcatc
    17281 cccaaagctc attccttggt agataatcaa aatgacatcc atcatcacag gaaaaataat
    17341 gacccacata gcagcggata acccttgttc tgagatccgt ttgcgtctga cgatcgttgc
    17401 catgagacca gaaaatacat aggacatcaa aataactgtc agaaagttcg tgccaatcga
    17461 atcataaaaa atgaaaagtg cggatactgc ttgaaaaata ccagccatga tccctgctct
    17521 tcgattcaag aaaaagctta aaacaagtgg agcaaaagcc gcggggtaaa acagcggaat
    17581 ataagcagct tgttctgttt ggaagagttg gaaaaatttc attagtacga cgcttactga
    17641 cattgccgtc acgtagaaaa gtacgtattt tgttctttct cctgcatttt ggaattgaag
    17701 tgaattataa atcagtacac cgatttgaag caaaacagct aaaaccatgg caatgagtgg
    17761 gaaaatagaa gtcgtttgat tcgtcaatcc tagtaattcc aattttttca tcgctgaagc
    17821 atcaatttga tttccttcgc gaacaataat ttctccttgg aagatcatca ctggttgtac
    17881 agcttctcgt gcagattgtt ttaattcttc tgtctttttc tcattcaata catcattaac
    17941 tacgatgcct tgatccacta agtagcgaat catttgttgc tgctctgttg tgacattcaa
    18001 atattgaatc tgatcttctg cttcttgttt aaaagcttcc aattcgcttt cacgaacttg
    18061 tttactcatc tgttcgtcaa tgagcttcaa actttcatcc ctaactgtgt gaatttccgt
    18121 ttcagtcatt tcaaatatcg tcgaataaaa attgttcggc agtttttggt aaaacgcaac
    18181 gttttctgca ttcacgtttt caaaattttt cttcagtgta gcaatccgct cttctgtagt
    18241 aggtttcggg atattttcat cttccttcgc cttatctacc ttgccattat aagatttgtt
    18301 gatggtatca ttcgtcttat cgatcaattc gaataattgc ttgatccgat tgttctggtc
    18361 atctgccaga tctttttgat aagtatattc tggagtcact gcttctgctg ccagttttct
    18421 tctttgttcg gtttcttccg tgttctcaat cgttttattc gcacggatac tctcttctgc
    18481 tacttggcct tctctgaaat caatggcctt ttgtttgaca ctactaaatg taacgccgca
    18541 gacaatgata aaaaacaaga acagaaggaa aggcaatgct gcctttccga gtttttcttg
    18601 tagtttctgc agtgttttat tcaacatgtg cttatcctcc taagcaaagc ttattcttga
    18661 tgaagatccg ccttttcata agctttgatg atttcggcaa ctacagggtg ccgaacaacg
    18721 tcacttgctt caaaattaac aaaatcgatt tttttgattt gctttaatgt tcgttctgca
    18781 tgaacaagac cacttgtcgt cccacgaggc aagtctatct gacttgtatc cccattgaca
    18841 atcatttttg attgataacc gagacgggtc aagaacattt tcatctgagc gactgtcgtg
    18901 ttttgtgctt cgtccaagat aacgaatgca tcatccaatg tccgcccccg catgtaagct
    18961 aaaggtgcga tttcgatcac gcctcgttcc atcaatcggt tcgtatgatc caatccaaaa
    19021 atttggtata aagcatcata aacaggccgc agataaggat ccactttttc tttcagatcc
    19081 ccgggtagaa agcctaggtt ttcccctgcc tccacagcgg gccgcgtcaa aatgattttt
    19141 tgcacttcgc ctttttttag ggcagcaatt gctaatacta cggcaagaaa cgttttccct
    19201 gttccggctg gccctacacc aaatacgatg tcatgtttgg ctaccgattc aacgtatttc
    19261 ttttgaccgc tgttttttac ccgtataggt tttccattac ggtctttgac gatctcttcc
    19321 tcatacatct caacaaaata atctaatgta cctttttgcc ccattttcaa tgccgtcaca
    19381 acatctggcg tacttacgtg gattcctcgt ttgatcaatt cctgtaatgt ccgaatgact
    19441 gaggcagcca aggaaatctg ggattgctca ccaatgatct gaatgatttc tccacgtgta
    19501 tggatggttg tttccgttgt ttcctcgatc agttttatat gcttgtcgtg tgcacctaaa
    19561 agcatgctga catcatcagc agctgtcaat ctaatctcta aagaactaga ctcttccgtc
    19621 aaaaatagac atcccctttt tttcgattca ttctttccta ttttaccata ctcagaagaa
    19681 actatatagc aaataagaaa aggttgtaaa agagtcgctt aaccatacgt accaagaaac
    19741 gttaaaaaat agccgtttcc attcgctaaa ggtctcgttt tgataaaaag aagctgagaa
    19801 tgtaagttat atgaaagtgg agaacaaaat atttcgtttc attcaacaag tattctctag
    19861 gtatttttcc tgtttcaaaa aaagaaaaca aaatagttaa aatattcttg catttcaaaa
    19921 aaaacaggtg tattattacg tcaacaaata aacatacagc aaagcgtcga ggaaaacagt
    19981 aggctattca acgatttatt cccagtgagt catgtaagtg agaaatgaca ataaatcgaa
    20041 tagacgaaca tcatttccga gtcaatgtcg aaaacgtaga tcattgtggc tgcctccatt
    20101 atcaggctag cggataactt tatcaaaaag cgtccgtgaa tgaagaagct tttttaagcg
    20161 aaaaaaggtg gtaccgttct agaatctaga gcccttttcc caagtggaaa agggcttttt
    20221 tttattttaa ggtggtggtt ttcatggaat ctgatattag tggcattact aattgctgca
    20281 acgtttatat cgtcaatcaa aatgataatt attggaggaa aaatttatgg aagaaacaaa
    20341 aacttattca tggaaacaac tattgatggt cagttcactt atcttcggga tgttcttcgg
    20401 gtcaggtaat ctaatctttc ctgtccactt aggtcaaatg gctggcgcaa actgggtcag
    20461 tgctggcgta ggatttgcta tctcaggtgc tcttttccca ttactagcta tcttagctgt
    20521 agtagtgaca aaaagtgatg gtctgtatga cttagctaaa cctgtcggaa aatggtatgc
    20581 tgccttgttc ttaatccttg ttcatttgac gatcggacca tttttcggta caccacggac
    20641 agctgctact gcttttgaaa tggctgctaa accatttttg cctgaaaaat ataattcatt
    20701 aggtatgttt ttattctctg ctcttttctt tttacttgct tatttcctta ctgtccatcc
    20761 aacacggttg gtcaaatacg tcggaaagta tctaaactcc ttgtttcttg cattattagc
    20821 aatcattttt gtcgtttctt tattccatcc aatgggaaat ctaaatcaat ctgctgcaga
    20881 tacttatcaa acaaacgctt taacaaatgg tgtcctagaa ggttataaca cggttgatgc
    20941 agtagctttg cttgccttaa gtgtcacttt tgtccatgca gtccgtgacc ttggtttcaa
    21001 agataaaaaa gtcacagaat taacggcaaa agctggtagc ctgagcgttg cgctagaagt
    21061 catgatctat tttggacttg ttcttctcgg tgcgatgagt ttgaataaga tgtctttatc
    21121 agaaaatggc ggaactgcac tttctcagat cactaatttt tatttaggta acttcggcgc
    21181 tagcttctta ggagtgatgg tcacattagg tgtcttcact accgctatgg gactcgttgt
    21241 ttcttttgcg caagatttcc ataaactgtt ccctaaagtc agttatatga catggctgcg
    21301 gttaacgact ttcgtctctt ttgtcgttgc aaatgctggt ttagacaata tcatccaatg
    21361 gtctttacca gtattgatgc ttttgtatcc attgtcgctt gcgttgatct tgctttcctt
    21421 aacagcaaaa tttttccaaa agacaccatt tgtctatcaa gtaacgatgt tgtttgctgc
    21481 tgttcctgcc gtattggata tgctggcaaa ttcgcctgct ttagtgagtc aacaaagagt
    21541 tgtagcaagc atgctcgaat tttatcatca tcacgttcct tttgctgcat taggacttgg
    21601 ctggatggtc ccaacgttac ttggttacgc tggcagccta ctgttttatt acgcctatcg
    21661 cctatcaggg tataaacaag aagcaaatga actaccagaa gaataaccaa atcaaacaca
    21721 atttaacata agaaatatta aacaggaaaa aaccagctag atgaaagaat cctattcttt
    21781 cgtctagcta tttttta
//
LOCUS       DAFEPQ010000043        21153 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.043, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000043
VERSION     DAFEPQ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.043"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..656
                     /locus_tag="LP543_001743"
     CDS             <1..656
                     /locus_tag="LP543_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2321910.1"
                     /translation="KIVLKTQSPLEVKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEV
                     IRKYDVQAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEYRNEIVELLLLAQERI
                     DATKQVTNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCTKDGQYEFYGRISELE
                     KKYPMLTRISRACLVNIFNVKEIDFKKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(1359..1574)
                     /locus_tag="LP543_001744"
     CDS             complement(1359..1574)
                     /locus_tag="LP543_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HBL2321911.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1593..1829)
                     /locus_tag="LP543_001745"
     CDS             complement(1593..1829)
                     /locus_tag="LP543_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HBL2321912.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            2087..4240
                     /locus_tag="LP543_001746"
     CDS             2087..4240
                     /locus_tag="LP543_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HBL2321913.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            4209..5624
                     /locus_tag="LP543_001747"
     CDS             4209..5624
                     /locus_tag="LP543_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBL2321914.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            5657..6691
                     /gene="sppA"
                     /locus_tag="LP543_001748"
     CDS             5657..6691
                     /gene="sppA"
                     /locus_tag="LP543_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBL2321915.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            6693..7214
                     /locus_tag="LP543_001749"
     CDS             6693..7214
                     /locus_tag="LP543_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBL2321916.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            7211..7597
                     /locus_tag="LP543_001750"
     CDS             7211..7597
                     /locus_tag="LP543_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBL2321917.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(8093..8165)
                     /locus_tag="LP543_001751"
     tRNA            complement(8093..8165)
                     /locus_tag="LP543_001751"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8130..8132),aa:Lys,seq:ctt)
     gene            8546..9115
                     /locus_tag="LP543_001752"
     CDS             8546..9115
                     /locus_tag="LP543_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBL2321918.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            9102..9962
                     /locus_tag="LP543_001753"
     CDS             9102..9962
                     /locus_tag="LP543_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL2321919.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            10152..10856
                     /locus_tag="LP543_001754"
     CDS             10152..10856
                     /locus_tag="LP543_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2321920.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            10861..12696
                     /gene="walK"
                     /locus_tag="LP543_001755"
     CDS             10861..12696
                     /gene="walK"
                     /locus_tag="LP543_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBL2321921.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            12693..14009
                     /locus_tag="LP543_001756"
     CDS             12693..14009
                     /locus_tag="LP543_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321922.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            14010..14879
                     /locus_tag="LP543_001757"
     CDS             14010..14879
                     /locus_tag="LP543_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HBL2321923.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESINAFSNT
                     IVTNNTVPKVEDQ"
     gene            14934..15743
                     /locus_tag="LP543_001758"
     CDS             14934..15743
                     /locus_tag="LP543_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL2321924.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(15846..16475)
                     /locus_tag="LP543_001759"
     CDS             complement(15846..16475)
                     /locus_tag="LP543_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBL2321925.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            16645..17937
                     /locus_tag="LP543_001760"
     CDS             16645..17937
                     /locus_tag="LP543_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2321926.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(18013..18675)
                     /locus_tag="LP543_001761"
     CDS             complement(18013..18675)
                     /locus_tag="LP543_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL2321927.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            18900..19184
                     /gene="groES"
                     /locus_tag="LP543_001762"
     CDS             18900..19184
                     /gene="groES"
                     /locus_tag="LP543_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBL2321928.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            19235..20860
                     /gene="groL"
                     /locus_tag="LP543_001763"
     CDS             19235..20860
                     /gene="groL"
                     /locus_tag="LP543_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBL2321929.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         7205 a         3427 c         4265 g         6256 t
ORIGIN      
        1 ttaagatagt attgaaaaca caaagtccgt tagaagttaa aaaatacctc aaacaattcc
       61 gaccaaagca aggaatatat ttcttagata ttgatttaaa tcatgaagtt aacggtatag
      121 aattagcaga agtaatcaga aaatatgatg ttcaagcaaa aatcattttt acaactactc
      181 atgatgagat gttacccgta acaataaaaa gaagagttga aacgttagga tttgtaacaa
      241 aagatcaaac actagatgag tatcgaaacg agattgttga gttattgtta ttagcgcaag
      301 aaaggataga tgcaacaaaa caggttacga atcaagcatt tgttttttcc ataggttctc
      361 aaacatttac ttttgatata aatgacattt attttgtaga agcttcaaaa ttaccacata
      421 gattatcatt gtgtactaaa gatgggcagt acgaatttta tggaagaatt agtgagcttg
      481 aaaagaaata tcctatgtta acaagaatta gtcgagcgtg tttagtaaat atctttaacg
      541 ttaaagaaat agattttaaa aaacgttcac tatattttga ttcagagttg gccagaaact
      601 ttactttagg gaaagcacaa aaaattaagg agaagttgaa agaaagcact gtttagtggt
      661 tttttcttta gggagattgc atttttctaa taaaatagat tccttcacta gtgcaaattt
      721 ttctgaatcc tattaaagtt gtaccaattt ttatagctgt tattagttac gtgcataaca
      781 gcaatattag ttactggagg aatatttcta tggtctggaa ccaaaaaagg gccaaatgaa
      841 tagttctttt cttgttttta ttaagtacca taaaggggaa ttaaaattaa atgtatggtg
      901 gaagatgcga aagatttggc aaattctact aatttttaga aagtgttttt cattttttag
      961 ttaaaaaatt tgtcctgtca agtgcaatcc tatgattgta cttgatagga tttgagataa
     1021 ttactttcct gtttttataa atttaacttg caggaacagg ctttaatgca catccgacaa
     1081 gtacattacc aatacctcct gtaacggccg aaataggtcc tcctgcagca ccaactacag
     1141 cgctgcaagt ccttttgtaa aaatatctct accattacat ttccaaagaa tttttatctg
     1201 actttaataa aagataaaaa tttaatataa ttataaaaac tagcacaaga agctttagta
     1261 aaaagttctg tccattccaa acttttgaaa agataaagaa catacctatg ttcgtaatcg
     1321 acacaaacca atcttgaggt ttgatatttt ttaattgttt acgcaccggt ccacatgctg
     1381 aatccagcac ctaataaagc tccacctaca ccacaagcag ctattgcata aggtcctccg
     1441 gctgaacaaa gttttactcc tgctgttgcc ccagaaaaag tagtgcctaa aactcccacg
     1501 gcactcccac cttgaatatt tttcaactct tctgaagata atataaatgt tgtttttgtg
     1561 tttttaatat gcatactact tattctcctt ttttattttt ttcgagcatc ccagccagct
     1621 gcagcacctc cagcgacagc tccatataac cctccaacaa caccgcaagc tggtccgcaa
     1681 attgctgttc ctaaacctgc accacctact gcccctgtgg taagtccacc tacaacttgc
     1741 cccaattttc cccaagcatt attgcctcca ccaacagttt gtgcatttcc acaatactta
     1801 gttcctttac attttgcatg tttaaacacg cttctttaaa taatattttt tacggtgcta
     1861 ctgtaacata cgtatttgat taacagtaaa aatcttcctg aaacttatca agtttattct
     1921 tgaatagtaa aatttccaaa ataaatttga ttcaggaatg aaaaagttag tgttcaagaa
     1981 ctgttttcaa agtttttgtg gaattatgag acaatatttt cactgtatta aagctatatt
     2041 gagaattttt ctataaaaat tttttagaag gagttgtgtt agataagtga taaaatcatt
     2101 tccttttgtg cagcaacaag atgaaaaaga ttgtggtgtt gcttgccttt cgatgatttt
     2161 aaaatatttt aaaactgata tcccgattca taaattacgg gacctatctg ggactgattt
     2221 agagggtact tcagcatttg gattgaaaaa gacatttgaa aaattgtctt ttgattgtat
     2281 ggctattcaa gcagatagtg agatctggaa agaaagagat cttatcttac cattaattgc
     2341 acatatattg gttgataaca gctatatgca ctacgttgta atttataaaa cgaagggaaa
     2401 cgtactatgg attgcagatc ccgctaatgg aaaaaataga aaaacgattg aagcgtttca
     2461 aaaagaatgg acaggtattc ttttattacc tatgcctaga gaaacttata ttcctattaa
     2521 agaaaaagta gctggattaa gttctttttt gcctataatt tggaaacaaa aaggattagt
     2581 attccacatc gttttagcat cgattttcat tacgtttttt ggcataggaa gctcatatta
     2641 ttttcaaggt attcttgatt ttttcatccc taatcaagca cgttcaactt taaatataat
     2701 ttccattggc ttgatcgctg tctatttatt tcgggtattg tttgaatata gtcgtagtta
     2761 tcttcttatt attttaggac agcgaatgag tatggcaatt atgcttcagt atttcaaaca
     2821 tgtgttaact ttaccaatga atttttttgc tacgagaaaa tcaggggaaa ttatttctcg
     2881 ttttcttgat gccagtaaga ttattgatgc tttagcaagt gctacgttat caatattttt
     2941 agatattggt atggtgttgc ttgttggggt tacgttagct attcagaata gtacgttgtt
     3001 tttaatcact ctatgctctt tgccttttta tgtagttgca atattcgctt tcgtgaaaag
     3061 ctatgaaaaa tcgaacaggg aagaaatggc agccggtgca gctgtaaatt ctagtatcat
     3121 agaaagtctt aaaggaatag aaacaattaa agcatatagc ggtgaagaga aagtatataa
     3181 ccgtatagac catgaatttg ttaaattaat gaaaaaatca tttcgtacgg ttacgcttga
     3241 taatattcaa caaggaatta aacatttgat tcaattaatt agcagtgcat taattttatg
     3301 gctaggttct tattttgtta tggataatag aattagtcta ggtcaactta tcacatacaa
     3361 tgctttacta gtatttttga cagatccttt acaaaacatt attaatttac aagtgaaaat
     3421 gcaaacggct caagttgcaa ataatcgttt aaatgaaatt ttcgcgattg attcagaaaa
     3481 atcgaaccag tcttttgaat atatgctttc tgaacaatta tttcaacaag atatcactgt
     3541 aaaaaacgtt tcattctcgt atagcatgaa ggccccaacg ttgaacgaaa tttcatgtac
     3601 aattccttcg cacagtaaaa cggctatagt tggagttagt ggttctggaa aatcaactct
     3661 tgcaaagtta tttgttcgtt tttatgaagc atcagaagga tcgatccatt atggaaacat
     3721 taatcactta gacattcctt ttcagatatt acgcgatcga gtgacatatt tgcctcaaga
     3781 gtcatttttc tttagtggaa caatcattga gaatcttata ttcggtttag atagcccacc
     3841 aagtttcgaa caaataatag aagtttgtga gatagtccaa ctaaatgaat ttattaatca
     3901 acagccatta cgttacgaga cattgttgga agaaggtgga aataatcttt caggagggca
     3961 acgacaacga ttagccttag cacgggcttt attaaaaaat ccggacatat taattttaga
     4021 tgaagcaact agtggattag ataccttgtt agaacatgca attatgaatt atctcctcaa
     4081 tttaaaagaa aaaacgatta tttttattgc gcatcattta ccaatagcaa aagcatgtga
     4141 tcaaatttta gttctccatg aagggcattt ggtagaaaaa ggaacgcacg atgaacttcg
     4201 ttttgccgat ggtatctatc aaaaattatg ggaaatctag gagagggaaa caatttatgc
     4261 agaataataa ttggttcgaa cattctagta tctatactaa acaacatcat agcttctacc
     4321 gttgggtgtt ataccctgta attctttttt tatgtattat tggattgttt ttaacctttg
     4381 ctaaaaaaga ggttgttatt cggacaacgg ctcaattgaa ccccgaaaaa atagagaagt
     4441 tacaagtgcc attagaagct aaaataatgg aaaacttcct aaaagaaaat caatttgttc
     4501 ataaggacga tgtgttggtt agattggatt gttctttaat agagaatgaa aaggctcaga
     4561 tagaacaaga aaaccaacgt attactcaac aaataaagat ggctcagcta tttattgaaa
     4621 gtataagtaa aggaaaaaat ttgttttcaa cggatgacag ttttggctac agtaatcaat
     4681 taaagagcat gttgtcagaa aaagaatcac tccgctacgc tttgaagcaa agtgaattaa
     4741 atgatcaaaa gcaattagaa gtatacgaaa agacaaaaag acaactagaa aaacaaattg
     4801 agagttcaga tagtaaatta caagaatggc aacaagtaca ggtagcttgg agtaataatc
     4861 aatcattaaa agatttttca aaagaaatga tggcaaacta tgagaattgg caagaacaac
     4921 taaataatgt ttctgatgat caaaaaaatc aagtgaaact gacaatttca gcaagcataa
     4981 atgaacaaat tgagcaacta aaaaaagaag tagaacagta tcagtcagaa aaagctaaat
     5041 tagttaaacc aactacttct gagaatgaca gaattagtca aacggaaaaa ggaaagcaag
     5101 agctagaaca agcaataaca actgtaaaac aaacagttac agagctaaat gaaaaacaag
     5161 agaagaacca gtcagttatc aaaagtttgg acgatcaact ttcaaaagga atactaaaag
     5221 ctcctgtatc tggaacggtt catttaaatg aagaaacaaa gggacaagca gaactagcca
     5281 aaggaacagt gcttgcagaa atttatccga tccacgaaaa atcaaaaatg aaatttacag
     5341 cacttttacc tgcgaatgaa agtacctata ttaaagaagg gatgaaggtt cattttaaat
     5401 tggatcaaaa aggtggggct ccgattacga tcgacggaac tttagatgaa atttcagaaa
     5461 attctactgc cactgagaaa ggtgtttttt atgcagtgaa aggaacgtta aaaacaactc
     5521 aatcgtttcc gtttcgttat ggattaactg gagagttgtc tttaattgta ggagaaaaaa
     5581 catattggca acaaattgtc gatgtgtttc taaataaaaa ataattattt ttactaagaa
     5641 tataggagga gtttatatga ataaaagaag atggattgct gtgggagtag cgtgcggatt
     5701 attgttacta tccattatcg tcgcgctaat tccaggaaaa gataaagagg aagcatcaaa
     5761 tacaacccta acaggaataa ataagatttt ttacggatct aacgaaatca cagaagaaac
     5821 gttagaagaa ggcgcatcaa ataaaaagat tgtcaaacta tccgtcaatg gagttattgc
     5881 agatacaggt gaaagcaatc tttttagtag ggaacaatat aaccatcaga attttttaac
     5941 acagctaaag aaaattcaag aagacaaagc ggttaaggga gtgttattag aagtaaattc
     6001 acctggggga ggaatatatg aaagtgctga gattgctaaa gaaatggcaa aaattaaaaa
     6061 gctagatatt cctatctata ctgcatttaa aaatacagcg gcaagtggag ggtactacat
     6121 ttctgctggt tcagataaaa tatttgctac ggaagaaaca acaactggat caattggtgt
     6181 cattatttct ggtttaaatt attctggctt attagaaaaa cttggcgtaa ctgatgcgac
     6241 gtataaaagt ggtgcattaa aagatatgat gtcacctcaa cacaaacctt cagaggaaga
     6301 gaataaagtt atacaggaat ttgtgatgag tgcttatgat cgttttgtca atgttgtagc
     6361 aaaaggaaga aatatggata ctaatgcagt gaaagaactt gcggatggaa gaatttatga
     6421 tggtaaccaa gcagtagaaa atgggttggt ggatcaaatt ggttactctg aagatgcatt
     6481 agattcccta aaaaaagaga agaaattaac tgatgcgacg gttatagaat ataaaaatga
     6541 cacaactggc tttgcttctt cttggttagg taataagatt gctgaatggc aagggctaaa
     6601 ggcaactaca tcagatcgtg taatttcagt ttttgaaaaa cttggatttt ctgatactcc
     6661 aaaaccaatg tattactatg gagggctata aaatgaataa gaagagtggc attgaaactt
     6721 tgcctaatta tgtatttgcg ggattttggt tacgattttt tgcttttctt attgatttaa
     6781 tttgtattag catcatcacc catgctacta taggtctctt ttacaaaatc atgggactgg
     6841 aatctgacaa atccattatt actttttatg gtatagcgtc attagctatt tatttaacct
     6901 attttgtact tttgactaag ctgaattcag gacaaacaat tgggaaaatg atttttggga
     6961 taagagtagt gagctttgag gaaaaagagc taagttggca aactatcttt attcgtgagt
     7021 gtatatgtag gtttatatta aaaaattttg tatttcaaat tgggtatttg gtggctgctt
     7081 tcacgcccaa gaaacaacat attggggatt atttttctaa cacgtcagtg gttgttattc
     7141 gttcattaga agctattaac tatggtcaaa aaatttctaa ttctaaaaca cttaatctgg
     7201 agagaattaa atgaataaga gaaaaacaga gtttattttg attttaatta gtggaataac
     7261 agggatttta tgtagtcttt tagcattgat gggtggtatg tttctatctt ctattgattc
     7321 aactacttta aatactacag aactaaccag taaggatata gatatgttag tcaatctaag
     7381 cgggaatgtt gttttatttg gaggaattac cctagttttt tcgataatga ttttcataat
     7441 aggtttttta gtaagaaaaa atcaaaaaac agtggtctat ggtattagtt tactcatttt
     7501 atctattatt ccattttttc ttttaacatt attgtgggta attcctggag ttttaggtat
     7561 tattgcggga ttgatgcttc taataagaaa atcttaatta ggcaatgtgt agacaacgtg
     7621 aaacgattaa tcaattaaaa gctaaatacg ttttgagaaa tgataacttg tagtcgatgg
     7681 aagacatgta gataaaaaaa ctacgtgtct tttatttatt ctaatttttt cgaaaaaaag
     7741 acactctctg attgttgtat taatggaaaa ggggaaagca gcaagtaaat ttagtttttc
     7801 tattaaatat tcttcacttt ttaacctttt atagtactta gatttaatcc tatatgttta
     7861 gagagttcat tggcagcatg attttgcaaa gcttctcgaa ttattgttat attagaaact
     7921 ttactgttca taattatgct tcttttttaa ctcaaagtcc aaagggctac agtggacttt
     7981 gagttaaaaa taaaaagccc tagttatttt gaaaaataaa aaaagaacag cgtaaatgtt
     8041 ttcgaaatgc tttcgcacca acatttacct gttccgtggg tttggcaccc aatgggccgt
     8101 gaggggctcg aacccgcgac ccgctgatta agagtcagct gctctaccaa ctgagctaac
     8161 ggcccgtaca aaaagtactt aataatagta gcactaagaa ttttaaaatg caagactaca
     8221 tttaaagaaa atcagaaaaa tctttacttc cttataggaa atcattcata gattattcac
     8281 atttgctcgc tattatataa gcataccaac aaacaaagtt cttgcaagag aacaaaccct
     8341 aacacttttt tcatttttta ctcctttccc gccgtctccc cggcgggtct cctgttctta
     8401 acgataaaag acaagaaggc ttagccttct tgttttttgt tttcatggaa tcccgctaat
     8461 caaatcacct agttaaagca actagattga atgtttatca taagtctgat atcatgttta
     8521 tgaaatgaaa aaaggagcgg ttatgatgga aaaaatgcgt cagcaattaa tggagtgggg
     8581 gaaggaacta gaaacattcc gtctgcctca ctgggaagaa ctccctgact tagaacttta
     8641 tatggaccaa gtcagaacgt tagttgatcg ttatctttca cctgtgatac aaggagaaaa
     8701 gcatccattg cttacctctt ctatggtaaa caattacgtt aaactaggtt tgataccagc
     8761 accagttaaa aaaagatata acaaagaaca cgtagcattt ttacttgcaa tcactacgtt
     8821 gaaacaagta ttgactattc ctgaaatcaa agaagggata ttgttccaag gaaaaacagt
     8881 aggaattcgt gaagcctata atctattttg cgatgaacaa gaagcagctg tgtggatggt
     8941 cagccaatta gcacaaggta aatctcatcc gcaaaaattc gaccaggcaa cccctgtaga
     9001 atatattgct gtaagagcgg caactttatc gtttgcaatg aaacttcttg cggaaaaaac
     9061 gattgttcta gaaaccgaat atttaaaaga ggagaaaaca aatgaaaaac aataaaatcg
     9121 caattttagt agattcaggt acggatgttc cagaagaatt gataaaaaaa tacgagatgt
     9181 atatgatccc attgaaaatt atttataaag atcaggtata tacagataaa attgacatta
     9241 cccctgaaga gatctatcaa cgtttgccag aggaaatccc tggtacatct ttgccggatg
     9301 gtgagtcgat cacagaaatc tttgagcaaa tcaaaaaaga cggttatgaa aaggtacttg
     9361 cggtaacaat ttccagtgga ttgagcggga catataatgt ggttcgttta attgcacaac
     9421 agcaagaaga attggaagca tatgtactag atacgaaaaa tattggtatt ggtgcaggtt
     9481 tttctgcgat ccaagcagca aaatggctgg aagaaggagt agaatgggat caactgatca
     9541 gtaatctgaa tgagttagtc gaaagaacaa aagttttctt taatgttgcg acattggaat
     9601 atcttcaaaa aggtggacgg atcggtttag ttgcttctat tgtaggaacc gctttaaagc
     9661 ttaacccaat catctcatgt aatgaagaag ggatttatta cacagtagga aaagctaggg
     9721 gacgtaaaaa aagtttggat aaaacggttg cttacgtcaa agaaaaaatc ggtaatgcaa
     9781 aggtcttcaa cttagctgtt gcccatgggg atgcgaaaga agaagcaatt gaaatggaag
     9841 cgagacttaa aagagagttc ccacaagcaa accaaatcta ctttggccag atttctccgg
     9901 cattagtagt ccacactggt ccgggattat tgggtgtcgg tgtacaagtt ttagaaggat
     9961 aaatcaaaaa aaggaaacac tgaggtgaaa atcagtgttt tcctttttta ttgaagttag
    10021 aacaaaaaat aataaagtat tttgcacttt cattttaatt ttctcttttg ttttataggc
    10081 aaagatgaaa caaaagcaga aatacggtat aatgagaagg tatgacaata atatataaga
    10141 ggtggctctt tatgaaaaaa gttttagttg tcgatgatga gaagccgatt tcggatatcg
    10201 tcaaattcaa tttagccaaa gaaggctatg acgtttatac tgcatatgat ggggaagaag
    10261 ccttggaaaa agttgctgag gtagaacctg atttgatttt acttgatttg atgctaccta
    10321 aaatggacgg cttagaagtt gctcgtgagg tacgcaaaac ttatgatatg ccgatcatta
    10381 tggtaacagc caaagattct gaaattgata aagtcctagg cttagagtta ggcgccgatg
    10441 attacgtgac caagccattt tccaaccgag aattagttgc acgtgtcaaa gcaaatcttc
    10501 gtcgcggtgc cacggctgcg aaggaaccag aagaggcagc acctgcggaa ttgacgattg
    10561 gtgatttgac gatccatcca gaagcttaca tggtgacgaa acgaggagaa acaatcgagt
    10621 taacacatcg tgaattcgaa ctactctttt acttggcgaa gcaccttgga caagtgatga
    10681 caagagaaca tttattacaa acggtttggg gttatgatta ttttggagat gtacgaacag
    10741 tggatgtaac tgtacgtcgt ttgcgtgaaa agatcgaaga taatccaagc catccaaatt
    10801 atttagtgac acgtcgtggt gtcggttact atttgcgtaa cccagaacag gagtaattgg
    10861 atgaaaagaa aagtccgctt ttttcgatca gtcaatttta agatcgctat tacctttatt
    10921 ctgattttat tgatctcgat cgaaattatt ggtgcctact ttatacgtgg tttagaacgt
    10981 tccacgatca atacatttat taaagatatg aatcaaacgg tggaatcatt agcaacaaca
    11041 atcagcccgg aattgaatcg aaaagataat gcagatgatg aagaggtaaa tgcgaatatc
    11101 aaaagattta ttgaaaacag tgcgactagc gatatcatcg aaatacgggt cgttgacgaa
    11161 aaagggatca tccgaggaac aaccgatgtc aatgaacaaa gcgctgtggg aaagaaaaat
    11221 gattatgttg atatcaatga tttcacgact aaacgttatg ttgctttaga taacgacgat
    11281 aaacgggtga atatcaatgt tcaaccgatc ctgtccccga ccggcgatgc cgttatcggg
    11341 gctctttacg tcaaaagtaa tatcgaacaa aaatattcgg aaatcaataa cacagcagtc
    11401 attttcttta ctgcctcact aattgcggca tttatttcaa tgatcgtatc tgttttagtt
    11461 gctcgttcga ttacacagcc gatcggtgaa atgagagaac aagccattcg aatcgctaga
    11521 ggagactaca gtcggaaagt caaagtgcat ggacaagatg aacttggaca gctggctgat
    11581 accttcaatc agttagctga acgtattgaa gaagctcaag atacaatgga agctgaacgc
    11641 aatcgtttgg atagcgtgct atcccatatg acagacggcg tcattgcgac agatcgcagg
    11701 ggtaaagtca ttacgatcaa tgatatggct gtttccttgc tagatatcaa aaacgaggaa
    11761 gcaatcggac aatcgatttt gacactttta gatatcgaag aagaatatac gttgcggaaa
    11821 ttattagaag atccaaacga aatggtactt gaccgttcaa ccagtacatt ggaaagtgat
    11881 cagatgatct tgcgagtaga cttttctatg atccgtcgcg aatcaggttt tatcagcgga
    11941 ttagtagctg tcttgcatga tgtgacagaa caagaaaaaa cagaacggga acgtcgtgaa
    12001 ttcgtctcta atgtttccca tgaacttcgt acaccgttga caagtatgcg tagttatatc
    12061 gaagcattga gcgaaggtgc gtggaaagac gaggaagtag caccaaattt tttgaaggta
    12121 acgctagatg aaacagaccg aatgattcga atgatcaatg acctcttaaa tctatctcgc
    12181 atggatacag gtaataccca gcttcaatta gaatatgtca atttcaacga gttagtgaat
    12241 tttgttcttg accggtttga tatgatggtg ggaaatcagg aaaaaaatta taagatccgt
    12301 cgtgaattta cgcaaagaga tctttgggtc gaagtagata cagataaaat catccaagta
    12361 gtcgataata tcatgaataa cgcgatcaag tattcaccag atgggggaac gattacttgt
    12421 cgcttattag aaacgcataa taacgtgatt ttaagtatca ccgatcaagg attggggatt
    12481 cctaaaaaag atctgaaccg agtgttcgag cggttctatc gagtagataa agcacgtgca
    12541 agagcacaag gtgggacagg acttggatta gctatttcta gagaagtgat taaggcgcat
    12601 cgaggcgcta tttgggcaga gagcaaggaa ggaaaagggt cgacttttta tatctcgctg
    12661 ccatatgaac cttatgagga ggaatggtgg gaatgagaat ctctgaaaaa gtcgttcgta
    12721 taggactcat tttgatggtt gcgttaagca tatacttctc ttatgcaatc tggctgagcc
    12781 cagcaggaaa aagttcaatc aacgtagatg agacaaacac tcaaatgatc gagtctcaaa
    12841 gcaatcgaaa aacttccgaa gtctttcttc cgcttcatgt tacatggcac cattcgggaa
    12901 tgatccaaga aaccaacagt gaaaatctcg tgtaccgatt gcaattgatc atcgataaag
    12961 caagatttgg aaagctttct gaagtagttt caggagacaa atcacagttt gaacaggtga
    13021 aaaatctgaa ctctggtttt gagttggcct ataatgcgcc ttttttgcta agagaataca
    13081 aggaagcatt tgatttagat ttagatgtgt cgaacttggc aaaagaaagc gaaaatatgt
    13141 attttactag aatacaattc gatcaaggaa ataaaaaaat ccgatttttg aattattcga
    13201 accatctcgt atatgaagca aatatttcgt tgaattggac agatatcaat aaagaattaa
    13261 cagcaaaaga cacaaaatgg acagaaatga caacagaacc atcgacgatc gacacgcaat
    13321 atgctacgaa acattcgatc aaactgaaga agtatagtta tatcctgtca cagcaaccct
    13381 atacactttt tagaaatgct tttttccaga aaccagatag cgtgaaaaat aatgatgaaa
    13441 gcaatgaatt gattttttac ggtgggaatg aaacaatgtc tatccattcg gaaacgcaag
    13501 tagttgattt tcgtggagag ctgccagaga aaaaagaaaa tgctagtgta ttttctgaaa
    13561 gtttccccta tattagcaaa ttaggcagtt ctttagggaa cctcagatat tttgaccgca
    13621 gtgacaatac gatcgattat cggatattcg tggaaggctt cccggttttt gggacagaca
    13681 gcagaggaca gattggtgtg gaagttggcg gtgaaacgaa tggttctgtg caagtgaata
    13741 tccaaaccag tttgaatacg attcaagtgc cgataccttc agacgaagaa gtagaacttg
    13801 caagtacgaa tgaaatcatc cagcagttaa aagctaaggg agcagatagc aacaagattg
    13861 gttcagtgat cgttggctat acatggcaaa atgtcaaaga aacgaatcgt gtcgttgatc
    13921 tcttacctga atggtatgtg aaatataaag ataactggta ttcagctaac gaccttctgg
    13981 aaaaattacc tggattggag gcgaattaat tggatttcaa aaaaatcgaa tggatatttt
    14041 tcgtcgcttt tttagggttg aatgtgtttt tattcagcag ttatcatgac gcggtatacc
    14101 aagaaaacaa tgtcattcgc tctaatcaga ctgaatcaat ccaacaacgt ctggctagcg
    14161 acgatatcac ttacacgggg aagatttcgt ctgagaacaa gcaaggctat tatcttagcg
    14221 cggagcagac caatcttagc acttcattag cgaattatcg caaggctggt aaaagcggct
    14281 tgctggcaag tggtgtgttt ttagacggtg atgttttgac gaaatcttta tccagttcag
    14341 atgctgaaaa gaacgtgatc gatccagata aaattagtag ttccttggaa gattttttat
    14401 ctaacaaaga caatatttta ttcggtaatg attatgtgta cacgaaaaac ttttctcatt
    14461 tagaagaaga accactagaa atccaagcaa gccaagagta tgaaggcatc ccattcaatg
    14521 atgatacagc aaaaatcgtt ctttctttag aaaaaaacga taatgattat agcatcacga
    14581 aatacatgca gacgcattta tctgatatcg agcagctgcg cgaaaagacg gaactgtata
    14641 cggaagagga agcaatcaat acattgtaca tcaataacaa aataccaaga ggatcaaaaa
    14701 ttttatggcg tcagctagct tattcctgta ttctgaaagt ccgggaaaaa aatgtgtatg
    14761 ttcctgtttg gcatgtagcg atcgaaacaa gcgataaagt ggtccaaatt gagagcatca
    14821 acgctttcag caatacgatc gtgacaaaca atactgtacc aaaagtggaa gatcaataaa
    14881 aaagcgagta taatgaaaaa agtttacaaa gtaagaaggg aagtaggagt caaatggctg
    14941 aagaaaaacc agcttttaaa atcagtatcc tagcaagtgg aagttcgggt aattcacttt
    15001 atatcgaaag tgaaaaaaaa cggcttttgg tcgatgcagg acttagcggt aaaaaaatca
    15061 cttccttgat ggctcaaatt gatcggacac cggaccaatt agatggaata cttgtgacgc
    15121 atgaacatcg agatcatata catggcgtgg gtgtcttggc tcgtaagtat catttggatg
    15181 tttttgccaa tcagcagact tgggaagcga tggaaccact gattggaaat gtaccagtgg
    15241 aacaaaaaca tctatttgag atggggaaag tcagaacatt cggcgatatc gatattgaga
    15301 gtttcggcgt ctcccatgac gcagcggctc cgcaatttta tcgtttccat aaagaaaatc
    15361 ggtcattcgt catgttgacg gacaccggat attgcagtga ttatatgcga ggtgtcattg
    15421 aaaatgccga tggttatttg atggaaagta atcatgatct tgagatgtta cgcatgggac
    15481 cttatccgtg gaatttaaaa cagcgtattt taggagatag aggacatctt tccaacgaag
    15541 acggtgcact tgtcatgact gatgtgatcg gtaatcggac caaacgaatc tatcttggcc
    15601 atcttagcaa ggaaaacaat atgaaagaac ttgctcattt aacaatggaa aatgttttga
    15661 aagaaaaaga ttttggcgtt ggacatgatt ttgaaatcta tgatacagat ccagatgaag
    15721 caactgagct attttcgatt taaacagttc aatcaataaa aatataaaaa gcagaagata
    15781 aaatcaatca gggttggttt tatcttctat ttttattttt ttagcatatt aaaaaaataa
    15841 ccgtattatc gactgactac actgatgtgc tgcttttcat gtttctcatc cagtatctca
    15901 tctactaatg caatcgcgta atctgccatg ctgatcgtac tgtcgctagc agcgtttctt
    15961 tgtaaataat catcacttaa caaataagta cctgtacgtt tgccatgtgg atcaaataaa
    16021 gaagctggac tgatataagt ccaatgtaga tttttagatt gtttcaattt taacaatcct
    16081 tcgtacatat tgaaagctgt tggatagaag ggtgcatttt taggtacttg gtcaatcata
    16141 cgagtcgtct tttctgtatc taagaataac gaagaggcac ccccaacgac aattaagcgt
    16201 gtatcggttc cttcaagcat ctcaatcaaa tgggacaaag aagtttgatg catttcttct
    16261 tttcctttgg gagcgttgaa agcatcaacg acaacatcaa aagatgcaag atcagtagtt
    16321 gtcaactcaa ataaatcctt ttgtataaac ggaacatctg atggcaattt ttctggatga
    16381 cgcacgattg ccgtcacgtc taaatctctg gacaatgctt cgtctaaaat aaatgaacct
    16441 gcatggccag tagctccaat gagtgcaatt ttcataaaaa acctcctttt ttattcctgt
    16501 ccattgtaag cagttgctga atattcgtca aagaatccga tttttgaaaa gctcagtgcg
    16561 cattcctttc atggttcgtg atcattgggt atagttaaat taagaaaact gattgataaa
    16621 aatagaagtt gaaggagata agaaatgaaa gagtggttag atcgtgcctt tttaccaatg
    16681 ataacatctg ttgtgaatct tagaccctta actgctttgc gtaatggaat gacttacacg
    16741 atcccaataa ttattgttgg ctcgctgttt ttcttatttg ctcattttcc tgtggattac
    16801 atcgctcagt ggatagacaa aacaggattt tctatgtatt ttacacaagt ataccaggct
    16861 acatttgatg tgatagcctt ttggatcgtt tttgccattg catacgctta tgcaaaagaa
    16921 gaagacatcg atcgcttacc agtcggcata acagccttgt ctgcttatct actgcttatt
    16981 cagccactaa atactcagat agatactgtc tggacaggtg ctgaagggat actaggagca
    17041 gttacaactg ggctcatcat cggatggggc tatgcacgtt tagtaagact tgcgaaaggg
    17101 tggaagcaaa gagagaagat acctgaaaat gtttggcagt cttttacttc attgattcca
    17161 atcattgtta tagtcacttt tacgcttatc ggttcagcgg tttttcaaca tttgttcaga
    17221 atgactgttg ttcaaggagt atggacatat gtccagcttc ccattcaatc gttcatagac
    17281 acgttgtttg gtgtgctttt attaggtttt tttgttccgt ttttatggtt atttggtctt
    17341 catggatctg cgatggtcaa tgggttggtt agtcccgtct tgcaagcgaa ttcattagca
    17401 aatgcagaaa tcttagcctc aggacaagag ttgacggtgg caaatggcgg acatatcgtt
    17461 actcagcaat ttctcgatca gttcatgacg gttacgggtg ctggattgac gttaggggct
    17521 gtatttttta tgacggtttt tgcgaaatcg aagaaataca gagaattagg aaatcttgct
    17581 cttttaccag cctttttcaa catcaatgaa tcaattattt tttccacacc gatcgtgatg
    17641 aacccgatga tggctgttcc ttttattttc gcaccggtcg tttcaggatt gattacctat
    17701 tccgctttat attttggatt cgttccttta tttacagcag tccaagtccc ttggacaaca
    17761 ccgccgatcc tctcgggttt cttagtaggc ggagtgcctg cagcgatttt acaaggtatc
    17821 gtgttgtcaa ttagtttttt catttatttc ccatttttga aaaaaataga ttctgcaaac
    17881 tgcaaaaagg aaagacagac aaaaaatgat ggagcagcag aaaagatgaa cgactaaaaa
    17941 acaaaaatac tcctgtcgct ttagattggg aaaatcacca atctaggatg acaggagtat
    18001 ttatagtcat gtttatacta gccctttttg gacagctaat tgaacaagaa caactaatgt
    18061 gttcattcct acgtgtgtga tcatcgatgt ccagatccga ccagttgctt tgtactctag
    18121 gctgaagaag aaacctagga aaaagtagat tagcagatga ccatcattat gagcaaacgc
    18181 aaacagcaaa gagctgatca ctgctccgac ccaaaaattt gaatatcgac tgatgatccc
    18241 cagtatactg cggcggaata cgaactcttc catgatgggt ccagcaacag ttgtcgccaa
    18301 aataaaagcg ggtgcttcca tgatcatctg gatgatattt tgtgtattct cggaaggggt
    18361 tgctttgcca aacaatacag attctaacat gacagcgatg ctttgcagaa tcaatgcgac
    18421 aaagattcca accaatccat aaataacaat ctttggagca gaagcttttt ttaccgctga
    18481 ttcgatcgct aagggttcac gttgcttaaa ataaaaaata gctaggacaa cggcgcctag
    18541 tatatacatg actgcagttg ctgtgatcaa ttcagacgta gattttacaa gtcctatcga
    18601 agctaggatc aagggtgaga taaaaattag accatagcaa aaaatgctga ggaagctata
    18661 tttttttaaa gacatgtaat tcctcctttt aagggataac aagcatgcaa ggcaagctta
    18721 tatcgattga tttcaattat atcataattt caataagcag ctgtcagaat aaaatttctt
    18781 ttctttgatt taaaagcttg caaaaaagag ggagaatgag tattattaaa aatgtaggtt
    18841 agcactcgaa ggttaagagt gctaacagat taaaaaaaga gttatcggag ggatttatcg
    18901 tgttaaaacc attaggtgat cgcgtcatta tcgaagtcgc aaaagaagaa gaaaaaactg
    18961 tcggaggaat cgttttagct tctgctgcta aagagaaacc ccaaactggg acagttgttg
    19021 cagtcggtga aggccgtttg ttagaaaacg gtgaaaaagt tcctgccgct gtaaaagcag
    19081 gcgaccaagt tatgttcgaa aaatacgccg gtacagaagt aaaatacgaa ggaaaagaat
    19141 atctgatcgt cgcaggaaaa gatatcatgg cgattgttga gtaaatttgg aagataaaaa
    19201 aataatcaaa aataatcaat gaggtgaatc aatcatggca aaagaattga aatttgcaga
    19261 agacgcacgc gcagctatgt tacgcggggt agataaatta gcagatacag tgaaagtaac
    19321 attaggacct aaaggccgaa atgtcgtact agaaaaatct tacggctctc cattgatcac
    19381 aaatgatggt gtgacgatcg caaaagaaat cgaattagaa gatcattttg aaaacatggg
    19441 tgcaaaactt gtatcagaag tagcatcaaa aacaaatgat attgcaggag acggtacaac
    19501 gacagcaact gttctgacac aagcaatcgt tcgcgaaggt ttaaaaaacg taactgcagg
    19561 tgctaatcct ctaggcattc gtcgcgggat cgaattggca acaaaagcag cagtcgaaga
    19621 attgcataat atctcaactg ttgttgattc aaaagaagcc attgcacaag tagcagctgt
    19681 ttcttcgggt tctgataaag ttggacattt gattgctgat gcaatggaaa aagttggaaa
    19741 cgacggtgtc atcacaatcg aagaatcaaa agggatcgaa acagaactag atgttgtaga
    19801 aggtatgcag tttgatcgtg gttacttatc acaatatatg gtaacagata acgacaaaat
    19861 ggaagctgtt ctagaaaacc catatatctt gatcacagac aaaaaaatct ctaacatcca
    19921 agatattctg ccattgttag aacaaatctt gcaacaatca cgtccattgt tgattattgc
    19981 tgacgatgta gatggtgaag cattgccaac tcttgtattg aacaaaatcc gtggaacatt
    20041 caacgtagta gctgtaaaag ctcctggttt cggcgatcgt cgtaaagcaa tgcttgaaga
    20101 tatcgctatt ttgacaggtg gtacagtaat tactgacgat ctaggtttag aattgaaaga
    20161 tgcaacaatc gaaaacttag gaaatgcttc taaagttgtt gtagataaag acaatacaac
    20221 gatcgtagaa ggttctggag aaaaagaagc aatcgaagct cgtgtgcaat tgatcaaaaa
    20281 ccaaattgct gaaacaactt ctgattttga ccgcgaaaaa ttacaagaac gcttagctaa
    20341 attagcaggt ggtgtagctg ttgttaaagt tggtgcagct actgaaacag aattgaaaga
    20401 attaaaatta cgcattgaag atgcattgaa tgctacacgt gccgcagtag aagaaggaat
    20461 ggtctcaggt ggtggtacag ctctagtgaa cgttatcagc aaagtttctg ctgtagaagc
    20521 agaaggagac gtggcaacag gtatcaagat cgttgttcgt gctttagaag aaccaattcg
    20581 tcaaatcgct gaaaacgctg gttatgaagg atcagtgatt gttgataaat tgaagaacgt
    20641 ggaactaggc actggtttca atgctgctac tggagaatgg gtaaacatgg ttgaagcagg
    20701 tatcgtagac ccaactaaag taacacgttc tgccttgcaa aatgccgctt ccgtttctgc
    20761 cttgttatta acgacagaag cagtcgttgc agataaacca gaaccagcag cgccagcagc
    20821 tcctgcaatg gatccatcaa tgatgggcgg tatgatgtaa gaaaaagcaa cttgcttatc
    20881 tacaactgat cattagtgaa agacagataa aaagaggctg gaacagaagc gtttaactcc
    20941 aagaaataag aagaaattca cggaaattgc ttttcaaatt tttgtgaatt tcagcttatt
    21001 tccgaaggag ttgcttctgc ttccgctgtt tatacgtgtt tagagcgtga gacaaaagtg
    21061 ttctttactt ttgtctcacg ctcttttttg tgtgtaatta taatgaaaat agttattttt
    21121 aatagttata atattttatc tattaaaagt aaa
//
LOCUS       DAFEPQ010000044        21114 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.044, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000044
VERSION     DAFEPQ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.044"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            474..725
                     /locus_tag="LP543_001764"
     CDS             474..725
                     /locus_tag="LP543_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321930.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            759..1037
                     /locus_tag="LP543_001765"
     CDS             759..1037
                     /locus_tag="LP543_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321931.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            1634..1891
                     /locus_tag="LP543_001766"
     CDS             1634..1891
                     /locus_tag="LP543_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321932.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            2004..2615
                     /locus_tag="LP543_001767"
     CDS             2004..2615
                     /locus_tag="LP543_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321933.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            2794..3351
                     /locus_tag="LP543_001768"
     CDS             2794..3351
                     /locus_tag="LP543_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321934.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(4050..4571)
                     /locus_tag="LP543_001769"
     CDS             complement(4050..4571)
                     /locus_tag="LP543_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBL2321935.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            4770..8462
                     /gene="nifJ"
                     /locus_tag="LP543_001770"
     CDS             4770..8462
                     /gene="nifJ"
                     /locus_tag="LP543_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBL2321936.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            8651..9538
                     /locus_tag="LP543_001771"
     CDS             8651..9538
                     /locus_tag="LP543_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBL2321937.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            9535..10704
                     /locus_tag="LP543_001772"
     CDS             9535..10704
                     /locus_tag="LP543_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321938.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            10728..12083
                     /locus_tag="LP543_001773"
     CDS             10728..12083
                     /locus_tag="LP543_001773"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBL2321939.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(12155..13960)
                     /gene="glmS"
                     /locus_tag="LP543_001774"
     CDS             complement(12155..13960)
                     /gene="glmS"
                     /locus_tag="LP543_001774"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBL2321940.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            14369..14659
                     /locus_tag="LP543_001775"
     CDS             14369..14659
                     /locus_tag="LP543_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321941.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            14672..15697
                     /gene="sppA"
                     /locus_tag="LP543_001776"
     CDS             14672..15697
                     /gene="sppA"
                     /locus_tag="LP543_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBL2321942.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            15700..16359
                     /locus_tag="LP543_001777"
     CDS             15700..16359
                     /locus_tag="LP543_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBL2321943.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            16516..17448
                     /locus_tag="LP543_001778"
     CDS             16516..17448
                     /locus_tag="LP543_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321944.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            17602..18891
                     /locus_tag="LP543_001779"
     CDS             17602..18891
                     /locus_tag="LP543_001779"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBL2321945.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            19138..20388
                     /gene="clpX"
                     /locus_tag="LP543_001780"
     CDS             19138..20388
                     /gene="clpX"
                     /locus_tag="LP543_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBL2321946.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            20471..21058
                     /locus_tag="LP543_001781"
     CDS             20471..21058
                     /locus_tag="LP543_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBL2321947.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT         7025 a         3605 c         4568 g         5916 t
ORIGIN      
        1 acaaataatt ggtttattct ggcaggcgta atgctttttt ctagtttcta tttatttagt
       61 aatgctctat tagcacagcg agggaaatat aagtttaaaa tattagatta gatattattt
      121 ttagagggga taaatataaa atgaaaagag gttatttata atggaaaaag tattaccaat
      181 taagagctac tgtattccaa ggcttttcat catagaattt caaaagtaaa gacatgtgtt
      241 tcacaaatag tgtctatcct ggctaaataa ttcttccact atctgcttta atttcagtag
      301 acttttcggc aagttatttg ctaatgagat gaatagagat tacaactcgc ctataaccgc
      361 ttaaaacaag cttttttaaa gtatagttag caagtcgtat tcatatgttc aaagaggagg
      421 gcttatgaac tttgaattca ttccgtgtga acagaacagt agggggaagt aaaatgaaag
      481 aaaagcctat aaataaatgg tcattgtata aaaattcaat gtttggagtt gttatctttg
      541 gtgtttgtgc tggattattc cttttcatat tattacaaac ggtgactctt ttctcttcta
      601 atcctactta tggatggaga gacagtatag ccttagggat gtttgcggga ggatatggat
      661 tcttattgtt gttctcaatt ctcacactaa gtccaatcca tggactcatt caactttgga
      721 agtaagaacg agccttaaac ttttcgttct cggaagaaat ggaaaaacac aacataacag
      781 gcagtatgta tgtggatgat caatggttta tcagcactga tgaggtaaaa atagctgcca
      841 ttaggagaga ttacctgcag aaaattcata aaataaaaga tcctaatggg aaaaaggcaa
      901 ggttaattat aactttgata acatctaatg gtaagaagtt aaaacttgca ggatcatatg
      961 ataatgttaa aaagttcgaa cattggtatc tgcaaaaaga cagcattagt cttaataaac
     1021 aatttgaaaa aaattaatca aagtcatcgg caacacgaat aatttgacat cttgatattg
     1081 tgatataagc aaaaacggca tttctgagac cgaacagcat aaaaaattta aatgttggaa
     1141 tttatataag caaaacccaa gaagctagat gcgatagagc atttaatttc ttggggtttt
     1201 gcttgggaat cagcatatca attaaaatga tcatctcggt atttcaaaag tgggtacgat
     1261 tacaaatagt tggagcaaaa tagtgggtaa aattaccatt ccagtgtctg tcttgcctct
     1321 ttttcgtcaa tcacaaatag acgagattca taggaaaaat cgatacaggg tatgatttca
     1381 atgatattat gaccaattac aaaatagatt gataaagcat tttttgcatt tttataatag
     1441 tttattgata ataatgtatg agatacagta taatgattta aaaggtaacg tgttgtgacc
     1501 gaattaaatg aattaatgcc tattttttat attccttagt ggacaaaaat ggagacagtg
     1561 aagttaggag cattgtatcc tttgttgcgt agttcaaaaa agtaagcgat ttctataatg
     1621 gtcagggtaa acggtgaggg ctgtccacgt aaatcctact attgaaatca caatgatcgg
     1681 acattcaaaa ggtggtgctg aagcagcaaa ttcagtagct ataaatacga atgcgattat
     1741 atttaatcca gcgacagtaa atttatctgc ttatgaatta gactcaaaca catatacagc
     1801 aaacatggta gcttatgttg tcgagggaga tgcattaaat gttgtttttg gaggagtata
     1861 cacctattgg agaattaatc atgttagata gacaatatga tgcaccttag tggagaatga
     1921 tatctcgcat aggtaactca gtaaaaaatc aaggtatgga agcaattatc tctgcattgg
     1981 aagatcgata tagataggcg gatatgaata gtaatagggg gaataaaatg gaatacaatg
     2041 gatcaatgag aattgtacaa attatatttt ggattacatc gggaattgtg atcataggag
     2101 gagttttttt gatgttgcca agcttaatat ttcctttctt tgcattaatc tcacctaaaa
     2161 tacccgaacc ggaaatcacc tatggagaat ttccatttag gattgaatac gaactagatg
     2221 gacagattaa cataattgaa gatactgtca tagctgagtt taacgggtgt gagttcagcg
     2281 caggttctat gaaaagagaa cgaagatgga gaagtagatt agctagtggt agagaagatc
     2341 taccttttgg ggacgatcta ggaatctatt ttagtcgagg aagtgcgcaa tattatatgg
     2401 gggaaaatgt acaatcaatg tcattaaaac cacatatagc ccttagaaat ctcgaggaag
     2461 gttttaaaag agaggtagac tttatattag gagaaagtgg atacataaga actgtactta
     2521 atttcggaga agcgcaagaa gttttgacac aatacggaat taccttaatc aactgggaaa
     2581 tatctgaacc gattgttaac aattttggtg attagaaaac aattacgata aatagaaaca
     2641 agccaaatca tcagcaagta aaagctgatg atttggcctt tcttatacga gtagtaggaa
     2701 ttcatagcga atattattac gaccagtaga tattttgata ttaaaggagt attacgaact
     2761 aatatgtgag aatgtagagg tgatgtgcga ataatgagaa atttatcaaa tagaaacaaa
     2821 atattaatca ttattgttgt tatagctgtg tttcatctgg gaacgaatgc ggttttaagt
     2881 agaataatat tgggtcctaa gccaccaaga ccagaaatta cccgagggga gtttgatttt
     2941 agattagaat atgaagtaga tggggaaagg attgtgatag aagatacaat tgttgcatta
     3001 tttgatggat tttctgcaga tgcaggtagc atggcatggt atcgcacgtg gcgtctacat
     3061 ttagcaagtg atagacgatc acgaaatatc ttattagatg aattagagga tggtcgaaga
     3121 atatattatg tcccagaaag tgctaactat tctatgggag atgtacagaa aaaaagagag
     3181 cctaatccta attggtatcc atttaatggc gtaacaatag aatatccacg caataaaaca
     3241 cctgaaatag gtgcaaaatt tataagtggt ttagaagatc tgtacgatag gtttggaatc
     3301 cgtcttatta gttgggaaca cgatccacca atagaaaatc gctttgaata atcgaaatct
     3361 actaatccat ttcatcagcc agtgaatgtt gtaatggaac aatttggttt agtgttcaaa
     3421 accattgaaa tactaggctg tgatcagcaa aaatgaatct gaataaaaaa gagagcaact
     3481 attagctgag aggagttaat agttgttagc tttatacgaa tcaaagtggc aaatacagat
     3541 gcgtatgcat ttgtgtttgc catcttgcgt ttggtagacg agataggtat aagctaaaga
     3601 aacaacttgt ttaaaataaa cgacctagct caacaaatga ccatctaatc aactaacagc
     3661 atacataaaa gcaactattt ttgaagtaga attgaaatcg ggataactta aaaagtgatt
     3721 gggatactag tgagaatcac cgtacataaa tattactcgt taagggaaat tgagaaattt
     3781 tgtatgaaaa tttaagaatt agttagtatg cgcaagtcaa aaatatgaat aaattatttg
     3841 gtggagataa tttgcttagc ttgtcataag aaatgagata atctggcaga tgcaagcttt
     3901 tggacaccat ttattcggaa taaggaggct gggacagaag cgtttaactc caagaaataa
     3961 gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc
     4021 tttacttttg tcccacgctc cttatttttt tattttgttt ggatcaattc ttttaattcc
     4081 tctattgaaa tagattcatc aaagatttct ttacccaaga tgacagttgg aacaaatcga
     4141 atatgtgctt gctcagcttc gttgatgaca gcttggctca gctgttcgtc tttttgttct
     4201 gtcagcttta gtttatctac tgcaaaatcc gctacttctt ggagagaaag atgtttccac
     4261 tcgtcttgag tatcgaatat ttgtttgatc tctttgatcg ctttttggcc atcgctgatt
     4321 gtgagatacc ggtgcatcac gttccctcgc tgtaagcttt ctttttcttt atcgaacaat
     4381 ttgatcactc gctgcaattg cccgctttgg actgcttctt ctaatagctc ataagattct
     4441 tcaaaccatt gtcggcaata cggacaagct aagttgatga attcgaccat ttttttagga
     4501 gcattcgatg aaccataaag gattcctttt tgtgtatttg ttttcgttgc atcaatcact
     4561 gatatatcca tgttaaaaca cctctttcgc ttatagttta tacatgtctg acaaaaaaag
     4621 gcaacgattt agactgtttg tctaaaaatt agatgaccaa ttcaaataga tacatgtaaa
     4681 atatttttat aaataaaaga aaaaaatggc atgttttacg ttataatgga acagataata
     4741 agagagaata tgaacaagga tgatgaatca tgcgaaaaat gaaaacaatg gatggcaata
     4801 cggcagccgc atatatttct tatgcattta ccgagattgc tgcaatatat ccaataacac
     4861 ccagttcgac catggctgaa ttagtagatc aatggtcagc agcaggaaaa gaaaacgtat
     4921 tcggtcaacc agtaaaagta gtagaaatgc aatccgaagc aggagcagct ggtgtggtcc
     4981 atggatcatt gaaaactggt gcactgacaa ctacatatac cgcttcacaa gggctgcttt
     5041 tgatgatacc gaatatgtac aagattgctg gggaactact gccaagtgtt ttccacgttg
     5101 ctagtcgggc agtgacgaca aatgcgttga atattttcgg agatcacgga gacgtgatgg
     5161 ctgcaagaca aacaggattt gctatgttag ctgaaagcag tgtacaagaa gtgatggatt
     5221 tagcacctgt tgcccatctt gcttctattg aaggagaagt cccttttttg aatttctttg
     5281 atggattccg tacgagtcac gagattcaaa aaatcgaagt tttggaatat gaagaattag
     5341 ctcctctgat caatcaggag aaattagctt catttcgtaa acgagggatg aatcccaatc
     5401 atccgactgt tagtggtacg aaccagaatc cagacatcca ttttcaacaa cgggaaacga
     5461 tcaaccccta ttatgataaa ttacctgaaa tcgttcaaaa atatatgaaa gagatcaacc
     5521 gattacgagg aacttcttat gacttggtga cttattacgg agcagacgat gcggaagaag
     5581 tcattgtttc tatgggatca gttgcacaga cgattgaaca aacgattgat tacttgaatg
     5641 caaacggacg gaaaacaggt tttttgaata tccatcttta tcgtcctttt ccagtggaaa
     5701 cattcgtgca aaagttgcct catacagtaa aaacaattgc tgttcttgat cgaagtaaag
     5761 aaccaggggc aggtggagaa cctctcttat tagacgttca aagtgcgtta tatgattctg
     5821 aacttcgccc ggctgttatt ggtggtcgct acggattggg gtctaaagat gtgactcccg
     5881 accaaatctc agcagttttt gatgaattga aaaaagaccc aagcatacgc aaaaaacggt
     5941 ttaccatagg gatcgtggat gatgtgactt atcaatcttt ggaaccaaag gaaagtctgg
     6001 atttgacaga accgcaaaca ttccaggcca agttttgggg atttggatct gacggaacag
     6061 ttggagcaaa taaatcagct atcaagatta ttggagatca caccgataaa tatgcgcaag
     6121 gctattttta ttatgattcg aaaaaatctg gcggtctgac tgtctctcat cttcgttttg
     6181 gagatacgcc gatacgttca gcgtatcttg tcgagcatgc ggaccttgtt gcctgtcaca
     6241 caccagctta cctgcatagc tatgacttgg tcaaaggatt gaagccaggt ggcatttttt
     6301 tattgaatac tctttggagc gacgaacaat tagaaacgca tctgccctta aaattaaaac
     6361 gttacttggc tgaaaataac attagattct atacgatcaa tgccatgaga ttggcccaag
     6421 aagtcggttt gggacgccgg atcaatacgg caatggaaac ggcttttttt aaattagctg
     6481 acatcattcc ttttgatgag gtgcttccgc tgctaaaaga agaagccctg aaaagctatg
     6541 gacataaatc aatggctatc gtagaaaaaa acatacaagc aatcgatcgg acgaccgaac
     6601 tgcttcatca agtcgcgatc ccagaccatt ggagaacgtt ggagatgcct gaaaaaatca
     6661 ataaaaggaa cactggttat atacaagaaa tacttgaacc agtgaatgca caggaaggaa
     6721 atcaactctc tgttggtaca cttgtagcaa atggaatgac aactggcgaa atgcctttag
     6781 gaacggcttc tatggaaaag cggggcgtcg ctttagaagt acctgagtgg attagtgatc
     6841 gctgtacgat gtgtaatgaa tgtgcctttg tttgtcctca tgctgccatc cgtccatttt
     6901 tagcagatga agaggaaatg gaagaagcgc cagaaggatt tatcgtgaga gaaatgcgag
     6961 gcgcagacgg cgtaaaatac cgtatccaag tttcagtgga agactgtaca ggttgtggtc
     7021 tgtgtgtaga ggcttgtccc gcaaaagaaa aagctttagt gatgaagcct tatgaagaac
     7081 agaaagaaca agctgtgaat tgggcttttg caatgacatt aagacaaaaa gcaaatccag
     7141 tcaaaggaaa gaatactgtc ttgtctacac aattcaatca accgcttctt gaattctcgg
     7201 gcgcttgctc aggctgtggc gaaacacctt atgtaaaatt attgacacaa atgttcggtg
     7261 atcgaatgat gatcgcgaat gcgacaggct gttcttctat ttggggcggt gcttctcctg
     7321 cttctcctta tacgaccaat gaatgtggtc aagggcctgc atggtcaaat tctttattgg
     7381 aagataatgc tgaatttggt tatggtatgc ttctagcggc acagactcgt cgcgaggctt
     7441 tggcaagttc aatcaaagaa gtattgccaa aagtatcaga agaactacga gaattgatgg
     7501 aagactggtt agtacacatg tttgagagtg aaggaacgca gcaaagagca gcaaaactgc
     7561 aagctgcgat cgaaagcgaa cgatcaattt tccctgaact ggaggatata tacaagagaa
     7621 aggatctatt cgtaaaaaac agccaatgga tgattggtgg agacggatgg gcttacgaca
     7681 ttggttttgg cgggatcgat catgtacttg ccagcggagc agacgtgaat atccttgttt
     7741 tggataacga agtctattcc aatactggcg gtcaaacctc aaaagcaaca ccagcttcgg
     7801 caatcgctaa attttcggct agtggaaagt atgcttcaaa gaaagatcta ggaatgatgg
     7861 cgatgacata tggaaatgtt tatgtggcgc aaattgcttc aggtgccaat caaatgcaaa
     7921 cgatcaaggc atttgaagaa gcagaaaagt atcctggtcc ttctctgatt attgcctata
     7981 cgccttgtat cacacatggc ttgcttggcg gaatgaaaga atcgcttaaa gaagcaaaag
     8041 aagctgtttc ttctggttat tggtcattat atcgatataa ccctacaatg gtcgaaaaaa
     8101 acaagaaccc aatgactttg gactacaaaa aacctgattt ctctgctatg cctgatttca
     8161 tgaggaaaca agttcgcttt tcctcgctag ataacgcaaa tccaaatctt gcaaaagctt
     8221 tatttgaaaa gactgtccac gacgctaaaa cacgttttta caactatgct gcactgacgg
     8281 gagaattaga aaaaatcaag aaacgacttg aaccagagga atctatcgat gtacccgtta
     8341 agactagaaa agaaaaaacg acggaccttg aagcagaaga aagaagagct gctagaagag
     8401 ctgccagagc agaacgacgt aaaaaaatgg aacaagctac aaaagaaaaa aaggaaaaat
     8461 aacagaaaaa atataatgaa taattctctg aagtttaaaa atatttattg cagagaaaaa
     8521 taaaacgcat acttgcaatt ttcttgaaaa ttatgcgttt ttctcaagaa aaatacgagc
     8581 atatcagcct ctaaaattaa aacatatgat atactagaga ggaaatctca aaagagaaga
     8641 gaggcttttt atgtcatttc aaatcgggga actttttaat ttggactatt ggcagcaaat
     8701 gttttccggt gacttgttct ctctgaattt tttggtgaac atacttgata ttctggttgt
     8761 ctggtatctt gtatacaaac tgatccaact gctgcgaggc acaaaagcga ttcagcttct
     8821 gaaaggtgta gctattttta ttgttatccg aatcgtagcg gaaatcattg gtctgcacac
     8881 cttgtcttgg ctgatgaacc aagtcattac gtacggggtg atagctgcta tcgtgatttt
     8941 tcagccggaa atccggagag gactggaaca tcttggacgc agttcgcttt tcaaacaatc
     9001 aaaaagcgaa cagcaggaag atgagaagat gatcttgtct tttgacaaag ccatccaata
     9061 tatgtccaag cgaaaaatcg gtgcgttgat cactatcgaa cgtcatactg gattagatga
     9121 gtatattgag acagggattg ctttagatgc agatatcact ggggagctgc tgatcaatat
     9181 ctttattcct aatacaccgc tgcatgacgg tgcagttatt gtaaaagaag gaaagatcgc
     9241 agtagcaagc gcctatcttc ctctatcaga aagtatgttg atccctaaag aattcgggac
     9301 tcgacaccgg gcagctgtcg gtataagcga agtcagtgac gcgattacca tcgttgtctc
     9361 tgaagaaacc ggggacgtca gcatcacgtt agataatgaa ctgatggctg gcttatccca
     9421 gcaagaatat ttggcaatct tgcgtagaga actaattgct gaagagaaaa caaaaaacaa
     9481 gaaaaacgtg attcaatctt tcctagacgg tatgactaag ggaggggatc gcaaatgatc
     9541 tcaaaagaga aacggacaag cattctttac agttttattg cattgctgtt cagtattgcg
     9601 ctgtacttca atgcaaatgg ccagtccgtt ccaaatacgc tttctggaaa tgaagcgtat
     9661 aatcaaacag ttacgggggt gccgatcaag atttcttatg attcaaagcg ctactatatc
     9721 catggatatg aaaatgcagt gactgtcaaa ctttccagtg caaaccgagt tcagttgaat
     9781 gcagaggcaa acgaagatac acggatgttc cgcgtcacag cagatatcac taagctgggc
     9841 gaaggaacac atgaagttcc gctaaaaatt caaaatttga gcagtgctgt cacagcaaag
     9901 atcgaaccat cgactattac agtaacggtt gagaaaaagg taataaaaga tttcgatgtg
     9961 gaaacgttga ttccttcaac gttcacacca tctggatatg aacttgatga tacttccgtt
    10021 agtcctaaaa aagtctctat cactacaggt gacaaaacat tggcagaaat caaacgagta
    10081 gtagctgatg tggacgcttc gatggtcaca gatgatggga ttaatagtga ggtcccaata
    10141 caggcgctga atgcagcagg ggaagtatta agtatcgtct ctgatccagt gcaagtaaca
    10201 gtgagagctg attcgatcaa gccaagcaag tcggtacgac tttatgggat acagcaagga
    10261 acaccagctg ccggggtaga aagctatgat ttcagttttt caaaactgga agcagaagta
    10321 agcggctcta gcgatttgct tgcatcgatc ggtgacagta ttgctgtacc gatcaatgtc
    10381 tcaggtatca atcatcgaac aactcgtaaa attgaaatac cagtagaaga ggggttaagt
    10441 atcaagcccg aatcagttag tgtggagatt acacccgtgt tgcaaacgtc aagcagtagc
    10501 tctgcttcga cgagcagtcg gacacaagaa aatacaagta cgagtagaag tatttcatct
    10561 gctgattcag gaacggaacc atcgacaaca accatttctt ccagttcgga atctgggaca
    10621 agcagctcga ccacaagtac agaagaaaca tcagaaacaa gcgaatcaac acaaatgagc
    10681 agaacaaatt cctcagaaaa ttaagttaaa ggagtaattt tacaactatg ggtaaatatt
    10741 ttggaacaga cggcgtacgc ggtgaagcca acaaggaact tacgccagaa cttgcattta
    10801 agctgggacg gtgcggaggt tatgtcctaa gccagcatga agaagaagga aaacgcccac
    10861 gcgtgttagt agggcgggat acaagaatct caggtcaaat gttagaagaa gcgctgattt
    10921 caggactttt atcggtgggt atcgaagtgt tccagctagg tgtgatctct acaccaggtg
    10981 ttgcttatct gacacgtttg caaaaagcaa gcgcaggggt catgatttct gcttctcata
    11041 atccagcaca agataatgga atcaaatttt tcggtgcaga tggttttaaa ctagtagatg
    11101 accaagagct agaaattgaa gcattattag atgctgaagt ggatgaattg cctcgtccat
    11161 cagctgaagg attgggaact gtagaagaat ttccagaagg cttattgaaa tattctcaat
    11221 tccttgtaca aacgatcaac ggtgaccttt ccggtttgac catttgtgtc gatgcagcaa
    11281 acggagcaac ttcaacaagt gttaatcgtt tgtttgccga tcttgaaaca gatttttata
    11341 caatgggcac aaatccagac gggttaaata tcaatgacgg cgtaggctct acccatccag
    11401 aacgtttagc tgagatggtc gttgaaaaag gagcagatgc tggactagct ttcgatggcg
    11461 atggcgatcg tattattgcg gtggatgaac ttggaaacat tgtcgacggt gataaaatca
    11521 tgtttatctg tgctaaatac ttggcagaaa aaaatcgatt gaaaaaagat acgatcgtta
    11581 cgactgtcat gagtaactta ggattccaca aagcggtgga aggaatcggt ttgaaagatg
    11641 tcgttacaca agttggtgat cgttacgttg tagaagaaat gcgcaaaaac gattataact
    11701 ttggcggcga gcagtctggg cacatgattt tcttagattt taacacaact ggagatggga
    11761 tgctttcagg aattcagtta ctgaatatca tgaaacagac aggtaagaaa ttatcagagc
    11821 ttgcagcaga ggtaacaatc tatccgcaaa aattagtgaa tatccgcgta agtgataaaa
    11881 acggtgcgat ggatgttccg gcaatcaaaa aagtgattga agaggcagaa gctgaaatgg
    11941 gatcagaagg aagaatccta gttcgtcctt ccggtacaga accattactt cgagtgatgg
    12001 cagaagcgcc tactgatgag aaagttaatt actatgtcga taaaatcgcg gcagtagtca
    12061 aagaagaaat cggactggat taagagatat tttcataaaa aataaaagaa caaggaccag
    12121 tgtcagatgt ctgacactgg tccttgttct tttattattc tactgtcact gatttcgcta
    12181 aattacgtgg tttgtcaacg tcgaatcccc gtaatagcgt tgcgtaatat gcaagtaatt
    12241 gagtcggtac gactgtggtt agagtagtta gataagggtg gacagttggt agcaccagct
    12301 ggtcactttc cttttctaat ccctctgaga caataactag tgttttagcg ccacggcttt
    12361 cgacttcttt caggtttcct cgagtatgcg caccagtcac ttcttcactg atgattccga
    12421 tcactggtgt tccttcttcg atcaaagcaa tcgttccatg tttcaattcg ccagcggcaa
    12481 aaccttctgc ctggatataa ctgatttctt tcaatttcaa agcagcttca agagaaacgt
    12541 tgtagtcagc accacgtcca atgtaaaagg catttcttgt atcgctgaga tatacttctg
    12601 ccaactcttc taaataatct ttttgggcga tgaccgattc catcgcatta gcaactaaac
    12661 tcaattcatg agtgatatcg aatacaagag aagtttcaaa ttctttttta tcaccgatag
    12721 ctttggctaa taaagttagt acagctattt gtgcagtata agctttcgtt gatgctactg
    12781 cgatttcggg tccagcatgc aatagcagtg taaaggatgc ttcccgcgaa agagtagaac
    12841 cagcaacatt tgtaatcgta agtgaagggt agccgagacg gttgattttt accagcactt
    12901 ggcggctgtc tgctgtttcg ccactttgac tcaagaaaat aaagaatggt ttttttgaaa
    12961 gaagaggtgt gttatagcca aattcactgg ctaaatgaac ctcaacagga atctgtgtca
    13021 atttttccaa agtctgttta cctgctaacc ccgcatggta actagttccg caagcaacga
    13081 tatagattcg atcgctatca gacaatgtat cgaccagctt ttgatcaaga gagacacgat
    13141 tgtcttctgt tacgtatttt tgagcgatac gacgcatgat aataggctgt tcgtcgattt
    13201 ctttcagcat ataaaaagga taggtacctt tctcgatatc tgaagcatct acttgtgctt
    13261 cgaatgaaga acgttctacg atgtcacctg caaaattttg aataataact ttttctgatg
    13321 tgacggtaac catctcttca tcttcaatct caacgaattc cttcgtatga gcaagcatag
    13381 ccattgcatc gctggcaatc acattgaatc catcgccgag tccgattaat agtggacttt
    13441 tatttttagc gacgtaaata acatcaggtg ctgtccgatc aatcaaagcg aaagcgtaag
    13501 aacctttgat gacccgcaaa gcttttaaaa acgcctcttg cgttgtagta tccttcgcga
    13561 aagtttcaat tagatgagcg ataatctcag tatcggtctc accaataaag tggtcatttg
    13621 ctaaataagc aattttaagt tcttcaaagt tttcgattac gccgttgtga acgagcacga
    13681 agcgtccact ttgggaagta tgtggatggg cgttttcttc agaaggttgc ccgtgagttg
    13741 cccatcgtgt atgaccaata cctatatttc ctgtagtatc aatatttatt ttgtttttca
    13801 aattttgaat tctgccttgt gctttgacca gataatcagt tgtgccatct gagacaaaga
    13861 taccagctga atcataacca cggtattcta atttttctag accgtcaatc aatcctggtg
    13921 ttacattttt taatccaatc attccaacaa ttccgcacat aaaaaacatc ctactttcta
    13981 taatgaattc gtatgaatgc ttccagctgc aggaaaatcc ttttgtttta tctttgcaga
    14041 cattttttgt ggattttctg tagagtgcag tccctagggc catccgccga atctttcgat
    14101 aaaccctctc ctcgtcatct gtttacacag acctggcgct atcctaaaag gtataactat
    14161 cctccttttt gttttttatc tggaaacatt taaaatcata accttttgcg aatgttcttg
    14221 tcaacaaaaa aagaataaaa aataaattgg tatatatcaa attctttgtc aaatgtcctt
    14281 tttatgtcaa aaaataatgg gtttttatct atcatctttt tcaggagttc ggtacaatag
    14341 aaagtaatcg gatagaggag gagtaaaaat ggattcaaaa attgagatca tgacactggg
    14401 aatgttaaaa aaacaattgt ccaaattcga agcatcagct ggtgtatcag atgatacgaa
    14461 aatattttta gatactggct gggatagcat ccaagaaatc gcacctgatg ccttggaagt
    14521 tgctcaagct cgtgagttta cggtagaaga tgaattaaca aaagagtcgt tttccgggta
    14581 tgcgcgggaa gaaaaagcag agcggttcga cacttctgag cagtcagaaa ctgtgatcgt
    14641 cataaaaaac ttgtattaga aaggagaaag aatgaataaa agacgttggg cagcagttct
    14701 gattgctgca ggattatttg tggtttcgtt agtttcagct agtttgacta agcctcagga
    14761 aaacgatcaa attgagggat tgaacagttg gttatatggg gatgaagaac tagctccaaa
    14821 tgtgttggaa gagggagaca gtacgcaaaa aatcgtaaaa ttatcagtag atggaacgat
    14881 tgctgcggga gggtcttcag gtttatttgc ttctgaaggt tacaatcata aaaattttat
    14941 ggaacaactg aaggcgattg aagaagatcc aacagtcaaa ggtatctttt tagaagtaaa
    15001 ttctcctggt ggcggtgtct acgaaagtgc ggaaatcgct aagaaattgg atacgattcg
    15061 taaagaacat gatatcccaa tgtatgtcag catgaaaaac atggcagcta gcggtggcta
    15121 ctatatttct gctcaagcag ataaaatctt tgcaacagaa gaaacagtga ccggctcaat
    15181 tggtgtgatc atgtcaggat tgaattattc aggacttcta gaaaaactgg gtgtggaaga
    15241 tacaacagtt aaaagcggag cgttgaaaga tatgggttca ggaacgcgac cagagacaaa
    15301 agaagaagaa gcggtgcttc aagcatatat cgacagcgca tatagtcgtt ttgtagcaat
    15361 cgtcagcgag ggacggaata aatctgaaga agaagtcaaa aaaattgctg atggaaggat
    15421 ttatgatggt gtccaagcaa aagaagtagg tttagtcgat gaattgggat ttcctgaaga
    15481 tgctttaaaa gctatacgaa aggaacaaca gcttgaagat gcggaactag tagaatattc
    15541 ttccacgcca tctgggtttg ctagtacatg gttaggaagt aaactagcag aattccaggg
    15601 attgaagcct tctgagacta gtcagatctt atccgttctt gagaatctag ggacagcgga
    15661 atcaccaaaa gcaatgtatt attacggagg tgagtaagca tgtcagagct ttcaaatgaa
    15721 aataaggaac aagaaaaaga taagcaacag cagcctgctg taggacctga ctccaacttt
    15781 tcccaaaagg tactgagctc ttttgaaaga gagcctttga cgcccgaaga aatcaaaaag
    15841 aaacaacgta aatggaaaaa gtatacggac acaagaaatg aaccgccaaa agcaaatgat
    15901 tttcctaagt atttctatgc aggttttttt atccgaatgt ttgccttctt ggtcgatctt
    15961 ttgtgtatcg gagctattac gcgtttaaca cttggtattg ccaaaaatct aggttggctt
    16021 aatttttcag attcttatct gagtctctac ggattcacag ccttacttat ttatctagcg
    16081 tattttattt tattgacgaa attcaatcat ggccaaacga ttgggaaaat gatctttggt
    16141 attcgagtca tcagctttga tgaagaagaa cttagctggt caaccgtttt agttagagaa
    16201 ggtgcttgcc ggttcattct aaaatttcct ttgctgatgt tagggtatct tccagcagtc
    16261 tttaatcaga aaaagcaaca tctgggcgat tttttcacta atacaagtgt tgttacgatc
    16321 aatctgatca aagcgttcaa ccaggaagtc aacagctgat gagcaataaa aagcagtaac
    16381 agttgaaagc accttctaaa agtcagatct aaaatctagc ttttagaggg tgcttcagtt
    16441 atttttaatt gtcatgcttt ttttgttttg ctactattga acatgagctg aatatgagca
    16501 agaggaggac actgcgtgaa gccgattgat tttccagaaa aatatgagaa tttaatgcga
    16561 gtggcacaac aagcactagc aaaccagcaa tatcagcagg caaaagaatt atttcaacga
    16621 gcttacgagt tgaaagaaag ctttgaggct aatagcctgc ttgttttttg tctttacgag
    16681 ttggacgaga aaaaagaagc attgaagcaa gcactcttgc atgaaaaaca gtatcttcaa
    16741 aatgaggagt ttgctgaatt ttattttgac ttgttaatca gtacacaaga ttttttatat
    16801 gctagaaaat tgatagcaag tacagatttt tatgaagctt tcgagcagcg ggtcattgaa
    16861 aaaatccaat ttgcagaaga attatccgga caaatggaga ggcagaagat caaggcactc
    16921 cacaataaat cggaggaact tccttcgttg gaacctgcac gtcagcttgc gtctattgat
    16981 gagatcgaac agcttcctta tcatgagttt atccaaacag cgagtaaact tattgtatcg
    17041 ccagaggtcc atattttagc aagggcaaaa ttactggaga cacttaggca attgaacgaa
    17101 tgccatcctg ttttctattt gacgattgaa gaaaagttag tcaaagtcat accaaaagat
    17161 ctgccaaagc cgcagcagca ggcaagttac cagcaattat gtgtattttc agatcgttac
    17221 gggaatgaag atgcgctgct tagttccgtg ttaaaagaag aattcacgtt gcaatctgca
    17281 attgtttatc ctgtctatga tacatacatc gaagacccga agcgctggtt tcaactgact
    17341 gtagaggcgt gtacaggaaa aacgatgtat gaaggtacag aagatgaaaa acaagatttt
    17401 ttaaaaaaaa gagaaaaaat tttgcagcaa atgatcttct tccattagat tttatcagtg
    17461 aaaaagataa aaatgttatg aaaaacaagg attctatcca ttcaatatgt ttacaatttg
    17521 aaagggttag tgtactattt aaaggtatgc atgaaacgtt tttgatgtat aaaatgaatt
    17581 tctaaactcg gagggaatta aatgactgca acatttgaaa aaaaaggcac gaatgatggt
    17641 gttttaaaat ttacgattgc gcaagacgaa atccaaaaag gattaacaac tgcgtttaat
    17701 aaagtgaaaa aaagcttgaa cgtacctgga ttccgtaaag ggaaagtttc tcgtcaagta
    17761 ttcaaccgta tgtacggtga agaagcttta tatgaagatg cattgaatgc tgtattacca
    17821 gaagcttatg aagctgctgt caaagaagct ggcatcgatc ctgtttctca accaaaaatc
    17881 gatgttgaaa gcatggaaaa aggtcaagac tgggtaatca ctgctgaagt aacagtgaaa
    17941 ccagaagtaa aattaggaca atataaagat ctagttgttg aaaaacaaga ccgtgaagta
    18001 acagatgctg acgtggatgc acgtatcaaa cgtgaacaag aagcacaagc tgaattagtt
    18061 atcaaagaag atgctgcagc tgaaaatggc gatacagtag taatcgactt cgaaggcttt
    18121 gtagatggcg aagcgtttga tggcggaaaa ggcgaaaatt actcacttga attaggttca
    18181 ggctcattca ttccaggttt tgaagatcaa ttagttggtc ataaatcagg tgaaagtcta
    18241 gatgtaaatg taactttccc agaagattat caagcagaag atcttgctgg aaaagaagca
    18301 gtcttcaaag tgacaatcca tgaagtaaaa gcaaaagaag tgcctgaatt agatgacgaa
    18361 ttcgcaaaag atgttgatga cgaagtatca acattagacg aattgaaaga aaaatatcgc
    18421 aaagaattag tagaaacaaa agaaaaagca gcggacgatg caaaagatga agcagcgatc
    18481 cgtatggctg ttgaaaacgc agaaatcgtt gaattgccac acgtgatggt tcatgatgaa
    18541 gtacatcgtt caatggacga attcttgaac aacatgcaac gtcaaggtat ctctcctgaa
    18601 atgtactatc aattaactgg ttctacagaa gaagacttgc ataaacaatt cgaaggtgaa
    18661 gcagaaatgc gcacaagaac aaatcttgtg atcgaagcaa ttgcagctgc tgaaaatctt
    18721 caagcaacag aagaagaaat caatgcagaa atccaagaat tatcagaaac atacaacatg
    18781 ccaatcgaac aaatcaaacg cgtgttgaca gaagatatgc tggaacatga tgtgacaatg
    18841 aaaaaagcaa tcgaattgat cacaggtaca gcgacagaag cgaaagaata gaaatcaagt
    18901 aataaatcat ttctgaagaa gctacttcta gaaaattgtt tattcgaaat aagggactgt
    18961 gacacgattt tgtcacagtt ccttattttt tgttaagagt aaaaaatgca aaggtatagg
    19021 tcagttgatc ctttgttaaa cactttttct ttttactaaa ctatgttatg atagtaaaag
    19081 actgtaaagt ctttgatagt ttcttgcata ctttttgcaa gttaaggggt gaagaccatg
    19141 tatgacaatc caagtagcaa tgagaccgta cgctgttctt tttgcgggaa aacccaagaa
    19201 gaagtgaaaa agatcgtagc tggtccaggg gtctacatct gtaatgaatg tatcgaccta
    19261 tgtaaagaaa tcattgatga agaattttat gaagaagctg tacgcgaatt cactgaagtc
    19321 cctaaaccac tagaaattct tgaagtattg aataactacg tgattggaca agatcgcgca
    19381 aaacgctcgt tagctgttgc cgtatacaat cactataaac gtgtcaatca gcaagcacaa
    19441 gaaaacacag atgatgttga actgcaaaaa agcaatatct gcttgatcgg acctacaggg
    19501 tctggtaaaa catttttagc acaaacatta gcaaaaacat tgaatgtacc ttttgcaatc
    19561 gcagatgcga caagtttgac agaagctggt tatgtggggg aagatgtaga aaacattttg
    19621 cttaaattgc ttcaatctgc tgattacaat gtggaacttg cagaaaaagg gatcatctat
    19681 attgatgaga tcgataaaat tgcgcgtaaa agcgaaaatg tatctattac tcgtgacgtt
    19741 tccggcgaag gtgtccaaca agccttgctg aaaattctag aaggaactgt tgcaagtgtg
    19801 ccacctcagg gcggacgtaa acatccacat caagaattta ttcagattga tacgacgaac
    19861 atattgttca tcgtaggtgg tgcatttgat ggcatcgaaa cgattgtgaa gaatcgtatg
    19921 ggcgaaaaga cgattggatt tggtacacaa aaccaaaaac tttctgaaga cgaaagtgtc
    19981 atgcagcata tcattcctga agatttacta aaattcggat tgatccctga gtttatcgga
    20041 cgattacctg ttatggcagc tttagagaaa ttgacaacag atgatctggt acggatcttg
    20101 actgagccga aaaatgcgct agtcaaacaa tatcaaaaac ttctttctct agataatacc
    20161 gaacttgaat tcgaacctga agcgttgaga gccattgcga aaaaagcaat cgaacgcaat
    20221 accggtgctc gaggacttcg ttccatcatt gaagaaatca tgatggatgt gatgtttgat
    20281 atcccttcaa atgaaaacat cgaaaaagtg attatcacaa aagaagcagc tgaattgtct
    20341 ggtgagccaa cagtaatcta taaagattca gagaaaaaag cgggttgatg aaaagaggct
    20401 gccgtaaaag tcattttggc ttttgcgaca gccgcttgct ttattcatca ccttgttaag
    20461 gagagagacc atgaaagtcc atcaagctga aattatcata agtgcagtgg caccaaaaca
    20521 atatccagat acacaattgc cagaaatagc tctggcagga cgatcaaatg tagggaaatc
    20581 ttcttttatc aacacattga ttgatcgaaa gaatttagcc cgtacttccg gtaagccagg
    20641 aaagacccag acattgaatt tttatttgat cgagaacgca ttacattttg tcgatgttcc
    20701 gggatatggg tatgcaaaag tatccaaaac tgagcgagca aaatggggaa agatgatcga
    20761 gacatattta acgactagag accagctacg tgcggtcgtt tctttagtag atttgcgtca
    20821 taagccttct caagaagata tccagatgta tcaatttttg aagtattatg atatccctgt
    20881 aattgttgtt gccactaagg cagataagat ccctcgtggt aaatggaaca agcatgagag
    20941 cgaaatcaaa aaagcgttgg attttgatcg caatgacacg tttattctct tttcttccgt
    21001 tacaaaacag ggaaaagaag aagcgtgggc agcaatcgaa caagcaattg aatcataaaa
    21061 agaataaaaa agacttacca gtcaaactct ttcataaaag tttggctggt aagt
//
LOCUS       DAFEPQ010000045        20856 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.047, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000045
VERSION     DAFEPQ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.047"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(84..263)
                     /gene="rpmF"
                     /locus_tag="LP543_001782"
     CDS             complement(84..263)
                     /gene="rpmF"
                     /locus_tag="LP543_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBL2321948.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(321..890)
                     /locus_tag="LP543_001783"
     CDS             complement(321..890)
                     /locus_tag="LP543_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBL2321949.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1076..1978
                     /locus_tag="LP543_001784"
     CDS             1076..1978
                     /locus_tag="LP543_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL2321950.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2013..3797)
                     /gene="pyk"
                     /locus_tag="LP543_001785"
     CDS             complement(2013..3797)
                     /gene="pyk"
                     /locus_tag="LP543_001785"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBL2321951.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3871..4833)
                     /gene="pfkA"
                     /locus_tag="LP543_001786"
     CDS             complement(3871..4833)
                     /gene="pfkA"
                     /locus_tag="LP543_001786"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBL2321952.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5001..8315)
                     /gene="dnaE"
                     /locus_tag="LP543_001787"
     CDS             complement(5001..8315)
                     /gene="dnaE"
                     /locus_tag="LP543_001787"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBL2321953.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8546..8731
                     /locus_tag="LP543_001788"
     CDS             8546..8731
                     /locus_tag="LP543_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBL2321954.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8793..9989)
                     /locus_tag="LP543_001789"
     CDS             complement(8793..9989)
                     /locus_tag="LP543_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL2321955.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10159..11595)
                     /locus_tag="LP543_001790"
     CDS             complement(10159..11595)
                     /locus_tag="LP543_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBL2321956.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVVTTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11888..12697
                     /locus_tag="LP543_001791"
     CDS             11888..12697
                     /locus_tag="LP543_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL2321957.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12883..15036)
                     /locus_tag="LP543_001792"
     CDS             complement(12883..15036)
                     /locus_tag="LP543_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBL2321958.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(15431..16279)
                     /locus_tag="LP543_001793"
     CDS             complement(15431..16279)
                     /locus_tag="LP543_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL2321959.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(16503..18443)
                     /gene="thrS"
                     /locus_tag="LP543_001794"
     CDS             complement(16503..18443)
                     /gene="thrS"
                     /locus_tag="LP543_001794"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBL2321960.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDIQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(18780..18959)
                     /locus_tag="LP543_001795"
     CDS             complement(18780..18959)
                     /locus_tag="LP543_001795"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBL2321961.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(19006..20829)
                     /gene="cadA"
                     /locus_tag="LP543_001796"
     CDS             complement(19006..20829)
                     /gene="cadA"
                     /locus_tag="LP543_001796"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBL2321962.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
BASE COUNT         5988 a         4449 c         3667 g         6752 t
ORIGIN      
        1 ttttttattt accgactaca aaaaaataaa cctcaaggct ccatggttag ccttgaggtt
       61 ttacattttc taattaaaat gtattaagct tcttttgaca ttacgtcctt accatcatag
      121 taaccacaat ttgcacatac gtgatggctt tttttcattt caccacagtt tggacattcg
      181 tttaaccctt tgattgtcaa tttgtagtgt gtacgacgtt ttgctttttt agcttttgat
      241 gttcttctag ctggtactgc cattttgcta cacctccttg taaagtcttg tatctaaatt
      301 agacacaata tattccaatc ttacttgtta tcgtcgtctt ttgatttaga atcaaaaagt
      361 tcagatagtt ttgcaagacg aggatcaatt ttattctcgg cttcttccat ttttctgcgc
      421 tggtattctt cttcggaaac aacttcccaa tcattgcctt ttggaagttc tgttgctgct
      481 tcctcttctt ttgtcaatac ccgcattgga atcgccaata agatgttgtc ttcaatcgac
      541 tctgctaaat tcaataatgg accttcaatt agcaaaatct catctgcaga catttgttca
      601 tctctacttt ggaattgttc ttctgtcatg aatacttcgt ctacagaaaa atccatgggc
      661 agttcaacag gttctagtga acgtgttgaa ggaactgtca aagtcgtctg cacagtgtaa
      721 tgaacgatat agcctttctt atcaactgag accaaacctt cgacttccac tggcgcaaca
      781 tcaagtatta aattgtctcg cttcatgaga gcggctttca agtcgagcgt ctcatgaaaa
      841 gttaacggtg tctcctggta ttttctcaat tccaataaag accatttcat ttttcatcac
      901 tcctaagcaa caaaagcaat tatacagggc aaaaagcatg ttgtcaatga aattttcttg
      961 acaccttgca gttcataaaa aaacagaaaa atctttatta gaaagcctct gtttgctatc
     1021 atcctcagaa tcatttacaa taatcattat tccattaaag gagcggatga tcttcatgaa
     1081 cacgaaagat atcgaatact atgtccaaat cgtcaaacaa aaaaatttta cgaaagtggc
     1141 taattatttc cgtgtgagcc aacctactat tacatatgca ttacaacgtc ttgaaaaaga
     1201 gtttgacacc gtacttatta caagagatcg atcgcatcac gaattaatcg tcacgccttc
     1261 tggaaagcag ctgtttctcc atgcacaaac cattctccaa gagatttcta ctgcaagagc
     1321 tgaaatcact cagttgcaaa agaaaaagct tcgttttggc atgcctccta ttatcggcaa
     1381 tgaatacttt cctaaactgt caagttactt tatcaagcat gaattgatga atcaaattga
     1441 gattaccgat ggaggctctc gggacttata cgggatgatc cgacaaggga ggatcgattt
     1501 agctgtatta ggctcaacac agccaatcgt ggatcaatat ttagaaacag agttactttt
     1561 ggacaaacgt ttcatgatcg tcgtgccacc tacacatcct ctttccaagc aggatactgt
     1621 ctctttcaaa gatatagcag atgaacagtt tgtactattg aacgaacatt atgtccaccc
     1681 cgcagcattc actaagcttt ctaaacaaac acatactcat ccaaaagttg tttatcaaag
     1741 caatgacttg acgattttaa agagtatggt ccgagaaaat gtgggtatcg gttttttggc
     1801 agagattgct gtccatcctg aagatcattt agtttgcctc cctctttcag atgaagttca
     1861 gcctcgcttc ttgatttcat tggcaaaacg aacacagcag atcgtcacgc ctcttcaaga
     1921 acaagtagtc caagttatcc atgaattcgt tacagcacaa aaaaacagct cttcgtaaaa
     1981 gaagagctgt ttttattttt tattaagcta aattagatag ctgttgtttc gccacggtaa
     2041 acgatgccgc gacgtgaatc aacggtaatc aattcacctt ctgtaattgt tgttgttgcg
     2101 cctttagcac caacgattac aggaatatct tgtgcaattc ctacaactgc agcgtgagaa
     2161 gtcaatccgc cttcttcaac gataacagca gctgcttttt cgattgcagg catatattct
     2221 ttgtcagttg atggtacaac tagaaccatt ccgtcttttg ctttttttac agcttcttca
     2281 gcagatgaag caataactgc atttgcaacg acagattttt gtccaacgcc ttgagcttct
     2341 aataatttag aaccaatcat ttgaattttc atcacgttag ttgttccgcg ttcaccaact
     2401 ggtacaccag cagtgatcaa gattaaatcg ccttctgatg cgaaacctaa ttcaacagct
     2461 tttttagctg ctagttcaaa catttcatca gttgttgatg gtttttcagc aactgttggg
     2521 taaacgcccc agtttaacat caaaccacgt tttgtgcgtt cgtcaaaagt aacagccaag
     2581 atatctgcat ctggacgata ttttgaaatc atttttgcag tgtatcctga ttcagttgca
     2641 gcaacgattg ttttcacgcc tagatttttt gcagcacgag ctacagataa accgattgtt
     2701 tcagttacat ctgttttatc gaattcgttc aattggaatg tacctaattc tgataatgca
     2761 ttttcagctt cttggtcgat acgtgccatt gttgaaacag cttcaactgg gtagtccccg
     2821 tttgcagatt caccagatag cattgttgca tctgtgccat caaatacagc gttagcaacg
     2881 tcagaagctt ccgcacgtgt tggacgtggg ttttcttgca ttgattctaa catttgtgta
     2941 gcagtgataa ctgggatacc agcagcatta cattttttga taatgcgttt ttgaaccatt
     3001 ggtactagtt cagctgggat ttcaacaccc atatctccgc gggcaatcat aacaccgtca
     3061 gaaactttga tgatttcgtc aatgttgtcg ataccttctt gagattcgat tttagggaag
     3121 atttgaacat gagtcatgtc tttttcttct aagatttcgc ggatatctag aacgtcttgt
     3181 gctttacgta caaaacttgc agcgataaag tcgatatcgt tgtctaaacc aaaacgaata
     3241 tcgtcagcat cttttggagt aatacctggc aaactaatag aaacgcctgg tgcatttacc
     3301 cctttacgtg atcctagcat acctgcattt ttaacttcta cgactaattc acggtttgct
     3361 tcgtcttttt caatgatttg catatcgatc aaaccatcat caaataaaat gtgtccgcca
     3421 acgtgaacat cgtcaaataa tcctgggtaa gtaaccgcga ttttttcttt tgaacctaca
     3481 tgttcaggat ccatagagat gcgtgtttga tctcctactt caaatttgat gtatcctgca
     3541 cgaccaaaat cagcttctgt tgtgccttga acagttgtac ggatttcagc tccttttgta
     3601 tctaaaagaa taccgacatc ttttcctgtc tttttaactg cttcacggac catagtcata
     3661 cgtgataatt gttcttcatg gtcaccatga gaaaaattaa agcggaagac atttgcccca
     3721 gattcaatca attgagaaat aatttctact gagttacttg ctggtcctaa tgtactaacg
     3781 attttggttt ttttcattta aaaaactctc ccattcaatt ttgtttattt aaagctgtac
     3841 acagcttaaa tagcttggtt tctttcactc ttaaaatgaa agtgaattat ttagatcata
     3901 tagtgataag tctggtttat gtttgttgtt ttcaagtgta tcaatgatgt cagacgcaac
     3961 gatttcgttg tcatggatac cgacacataa tccacctttt ccttcttgaa gcaattcgac
     4021 agcgtaagca ccgaatttac ttgccaacac gcggtcgcgt gcgcttggtg ctccaccacg
     4081 gacaacgtgt cccaagatag atacacgtgt atgatagtca ccaaattctg aaagtttctc
     4141 agcgaattca tttccgccca ttacaccttc agccaagatg atcaagcaat gtttcttccc
     4201 gcgatcgcgg ccttcttgga tttttttagc aactgaagcc atatcaaaat catgttctgg
     4261 gatgatgatt tcatccgctc cgccagccac acctgaccat aaagcgatgt ctcctgcatt
     4321 gcgtcccatt acttcgataa cgaatgtacg aacatgagaa gtcgctgtgt cacgaatacg
     4381 gtcaattgat tctagtactg tattgattgc tgtatcaaaa ccgattgtga aatcagtccc
     4441 aggaatatca ttatcgattg ttccaggaat acctactgct gggaaaccgc gttttgtcaa
     4501 agccattgcg ccatgataag agccgtctcc tccgataacc actagacctt cgattccgaa
     4561 ttttttcaat tgttcgattc cttttaattg accttcttca gttgcaaatt ctggataacg
     4621 tgcagaatac aagaatgtac caccacgttg gatcttgtca cctacatctg ccacatcaag
     4681 acgacgaata tctccagcaa ctaaaccggc aaaaccataa ttgatcccgt atacttccag
     4741 tccttcgtaa atacctttac gaacaacggc acgaacagct gcattcattc caggtgcgtc
     4801 tccgccactg gtcaaaattc cgatgcgttt cataataaac attcacctca ttaaaatact
     4861 ttatcctaag tatgacttag gtttacaacc atttttgcgt taaccacgac taacattcta
     4921 acattatttt tctcacttgt cttgatttac ggataaaaaa atgaaaaaaa atgaaagcaa
     4981 gtagtatttt ttaccgtaag ttatttgaaa accacattat cttcgccaag cagataaata
     5041 agttgcccct ttatactctt gctgtcatcc acccaataag catctgacag aactactttt
     5101 cgttgcgttt tttcgtgata cataactact ggtatatatc ctgggtgttt ctgaaaaatc
     5161 acttgtaatt gtttcattat ctcgggatct tcccgttctt ctgtaatacg caaatagcaa
     5221 gtttgtgaag gatactgggc ttctaactgg ttaggatcag caaagctttc tccaattagt
     5281 tgcagttcct gattatattt gctgatttcg ctccgccctt gaacaaagat cactcgatca
     5341 acggcaaaca aattacgata tttacgataa agagttggaa aaactgtgac agaaatttct
     5401 ccactactgt cggttccttc aacaaaggcc atcagttctc ctttttttgt ccgaatttct
     5461 ctaacttcct tgatcatcac taataaattg actggttgat tagggacgat atctgtaatc
     5521 agctgaactt gtttagctaa acgaagacga tcaaaccctt ctgcaggatg acctgacaaa
     5581 taaataccta agtactcctc ttccagcttc aagcgttctt ctagtgtata gtcagcgact
     5641 tcttcttctt ttaatgccat gattcccagc aagtcaagac ttccaccact gtaaacgata
     5701 ttttggattt ttccttcgag atcaagcatc aattgtttcc tgttaggaga taattcatca
     5761 aaaactccta ctgctgtcag tgattgcagt aactctaatt tcagccaacg cttatcgatc
     5821 cgtatgagga attgatccac tgactggaac gggccatttt gttttctttc gtttaatata
     5881 tcttctacaa aatcacgccg aatacctttt atcgctgtca gtccgaatct gatttcttcc
     5941 ttattgacca actcaaaact ggaattactt tgattaatgg acggcgagga aagtttgagt
     6001 ttatttttac gtgcttctgc aatatattct ttcaattttg atgtgttatg ccgaacagaa
     6061 ttcatcaggc tggtaaagaa agcacctgga tgatgggctt ttagatacgc catttggaat
     6121 cccacgaatg aataagcaaa tgcatgtgac cgattgaatc catagtttgc aaatttttcg
     6181 atatattcat atacggtcgc tgctgttttt tccgaatatc cctgttgtaa tgctcctgat
     6241 acaaaatgcg acttttcttc atctaatacg tcttttttct ttttactcac tgcccgtcgc
     6301 agaatatctg actgccctaa tgtaaatcca gccatagtcg aggcgatctg catgatttgt
     6361 tcctgataga cgatcactcc atatgtgttt ttcaaaatcg ggatcagtga atcatctggg
     6421 tagcgaatgg cttcttttcc tttcttcctg gcaatgaagg tatctatatt ttgcataggg
     6481 cctggtcgat acaatgcatt gactgcggca atatcttcga tgttttctgg atctaatctt
     6541 cgtaaaacgt tccggatacc agcagattca aattgaaata ctcctgaggt ttcgcctcgt
     6601 tgaaacaaga gcagcgtttt tgcatcattt agcgggatct cttttaatcg gatctcatac
     6661 ccttctactc gcttaacagc tttcaaagta aaatcaataa tcgataagtt gcgtagtcct
     6721 aaaaagtcca ttttcaaaag accgattgct tctacctcat tcatcgtata ctgggtcaga
     6781 aaagcttcat cagaaccgcg ttggagcgga actagttcaa gtagattctt atcgctgata
     6841 actacacctg ctgcgtgagt cgaaacatgt cttggcaatc cttctaaaat cactgcagtt
     6901 ttgaataaaa gttggttttt ttcgcttgcc tgaactaatt ctactaattg atgcgaagtt
     6961 ttataagctt gggtcaatgt gattttcaat tgattgggta ttgcttttga ccagcggttt
     7021 gcttcacttt gcgataaacc aaacactcta gctgcatctc taagaaccat ttttgctgcc
     7081 attgtgccaa atgttgcgat ttgtgccata tgatattgtc catacttttc tcgaacataa
     7141 ttcaatactt cttcacgtcg attgtcagga atatccaaat cgatatctgg catcgtaaag
     7201 cgttcggggt ttaaaaagcg ttcgaacagc aaatcatatt ctaatggatc cacatctgtg
     7261 atttctaaca cataggctac taatgaaccc gcagcagaac cacgacctgc accggtaacg
     7321 attttgtttc tatgagcaaa agccattaca tcccatacga tcaagaaata atcatcgaat
     7381 cccatttgat ggatgacaga taactcgtag tccaaacgtt cttggtattc ctttgtatag
     7441 tcagtgattc ggcgttccac ttgcgaatag cataactctc ttaaatattc tccagcttgt
     7501 tttccattag gaactggata atgcggaagt aatttctgat ggaactggaa ttcaaatgaa
     7561 atcgattcag ctaaacgctg ggcgcctatt acagcttggg aaagacccgc tttttcgaat
     7621 cgtttgatca tttcttgttc tgaaagaaga tagacatttg ctgttagtgg taaagatgca
     7681 gataaatttt ctaaaggtat ttggcttccc tctttgatat gtttcatcac ttcaagtgca
     7741 aaaagttctt cttgttgtaa actagcgatg ggatgtaatg cacaaagcga ataattttct
     7801 tttatcacat actgatacca ttcaggcaat tgatcaggcg tgttttctgt caagccgata
     7861 taaaaatttc tagtaaaaac attttcaagt aaaaactgcc aatgaccgaa aaagttatcc
     7921 tctttattga aagcctcaga aacagaatga tttgccggaa caacaaacgc tagatggtcg
     7981 gtttctgtca tttctgcaag ttccaatttt tcttggatca tttttcggct ggaaagtttc
     8041 atcagctgct gataacctat ttcatctttt gcgtacgcaa gcaagccata atttttttct
     8101 tcataagtat aactaagatg taatccaatg attggctgta ctccttcttc tacacaaata
     8161 cgatagaatt caggtgcacc gtataagaca tctttatccg ttatggctaa tgaagtgtag
     8221 ccttgttttt ttgcttcttg tacatattct cgaatacgta tcgtactttg taaaagagaa
     8281 taagaagtaa ttgtataaag ttgtgggaaa aacatcttgc attccgcctt ttttttcgtt
     8341 aaaattcact agcgttaaaa ggtttgatcc ttttactttc attaagatag caggcgctga
     8401 agttgattat tccacttcat ttgcttgctt gtaatctgta ggaatgagtt ctttcgcaag
     8461 aactcaaatt ttattgagat ttggtctcgt ttctttacat tcaaagaaaa tctggtaaaa
     8521 ttaagataga aaagaggtgt tctttatgcg atatatcgtt acattattct gggctgtcgt
     8581 tctaggtcaa gtagtcggtt atattggtgc tgcattaaca agcggaacat atgattttac
     8641 tttgacaaca atcatttcat tcattgcggg tgtcattatc cttttgattg gtgcagttgc
     8701 gccaagaaaa gaaacctctg cccattcgta aaaaaacagc caagagcttg agatttctca
     8761 agctcttttt tattttctac tcctcaccac cattataatc tgctgcgaag aactttacga
     8821 aagttgaata aagaaagaag aatatttgac agaacaaaca aacttgtcac aataaaaaca
     8881 gcactgtaat tataagcatg cgcgactgtc gatcctagca tgggacctaa aacttgtcca
     8941 aagttgctaa acatctgatt gtagctatag attcggctga ctccttcttg aggcgtaatc
     9001 ttactgatca gtgtattgac agaaggcatc aaagcgccag ttgaaaagcc taaaataaag
     9061 cgaagtatac ctaattggag cggtgtagtg acgaatgcca taggtaaata acagcaaaat
     9121 gataaaatga gtcctccaat tagaactttt tgattcccaa tcttatcccc tagttttcct
     9181 agtttcggtg aagaaaagac agcggaaaca ccagctatcg aaacaatcaa gccgctgaca
     9241 aaaaggatat tttctgtact tccgctcaat tcacgaatat ataaggtcaa aatagggctg
     9301 atcgtagtga ttcctatctg taaaatcaag gtcgtaatga agagaccgat caaaatagag
     9361 agatgatcca tttttgaaaa aatttctttg gtgcttatca gatctttttt ctcgattgga
     9421 tggaaatctt ctttgactaa gaaaatggtc aataccgttg tgatcatcaa caaagcccct
     9481 gtgatcaaga atacattttc cataccaaac cactgagcca gtgcaccgcc cattgaaggt
     9541 ccgatcagat ttccagcaat cgctccggta gacaatgttc ctaacgccca tccgctcttt
     9601 tctctaggtg cctgtgaagc aatcatcgct gtggcatttg gaatatatcc agacaaaatc
     9661 ccattccaaa accgcatgat caaaagccaa tacgcgtttg gtacaaaagc caaagatccc
     9721 attgttaatg tcatccctgc tgctgcacgg atcatcatga gttttcggcc ttttcgatca
     9781 gctagatttc cccagattgg tgcaaccacg gcagatgcaa aagcagtcac agagattgct
     9841 agtcccgaaa acaattcgat ttgatctttt ggtgttccta gttgctcaac ataaactggt
     9901 atgaatggca taactaaact aatacttgca ccagtgaaaa aacagccaat ccacgcaatc
     9961 actaaatttt ttctccaatt aatctccatc tatcatccct ctttcgtctc ttttatcata
    10021 cctattttcg aataaacaag caacgatctc gccagtaaaa agagaaaaat cccgcaaagg
    10081 tcattttata tgtaacgttc tttttatgta aaacaaaaag agatcagaac aaatactgtt
    10141 ccaatccctt ctctttagtt aaattgttgc tagaatgatg aagttgataa tgaataaaat
    10201 atcaacgatc cacaaagcaa cagaaacttg attgtatttt cctttgatta ctttcacaat
    10261 tgtatagaaa ataaatcctg ctgcaatccc ataggaaatg ctatagcaca atcccataaa
    10321 gatcgatgca aagaaggccg gtaaagcttc ttccatatct agccatttga tgtctgcaaa
    10381 agaagccatc atcattactc caactaaaat caatgctggc gcagttgctt gatttggtac
    10441 aatggcgatc agtggagaga ataaactact taatgcgaat agcactgcca ctactactga
    10501 agtcaaacca gtacgtccac cggccccgat cccagctgca gattcgacat aagttgttgt
    10561 attcgatgta ccaaagatag cccccacaga agtagcgatc gcatcggcaa atagtgcttt
    10621 atccattttt gttttgaacc cacgaccatc ttccaacgcg atctcgtctt cttttgagaa
    10681 aatacctgtg cggcgtcctg taccgataaa tgttccgatc gtatcgaaag tatcagataa
    10741 actgaatgcg atgatcgtca tcaatacttg aggaatcttg gaagaatcac tgaataaaga
    10801 ttgcatgcct tcagaaccaa atgctgcacc gaaagttgtt cctaattctt taaatgaatt
    10861 acctaacgaa ttcgtttgcc aatcaatgga acttaaatca acaacaccca taaaaatacc
    10921 aaggactgtg gtcactacga taccgataag aatagcccca cggacatttt ttacaactaa
    10981 aatggctgtc agtaccaaac caatcacagc caataagata ggagcgttgt caaagttggc
    11041 taacgcaggg acgattccac catttatcgt aacatttgtc gcttttccgc cttcaacgac
    11101 agagctcgaa atagctgact ggtctgctga aaaagataag aatcctgcat ttttgattcc
    11161 gacatacgca acgaaaatac cgatcccgcc accaatcgca tgttgaagac tttcgggaat
    11221 cgcccgaata atcatttttc gaatatttgt gacagtaata aaaatattga ttaatccgca
    11281 aataaacacc attgccaagg cttgttgcca agaataacca agcccaaaaa cgaccgtaaa
    11341 agtgaaaaat gcgttcaatc ccataccagg tgcctgagca tatggtacat ttgcgaacaa
    11401 tcccattacc agcgtaccaa taatcgatgc aatgattgtc gctagaaata ctgcttggaa
    11461 gggcatacct gatgctgaca aaatcgtcgg atttacgaat aagatataac tcatggcaaa
    11521 gaatgtcgtt actcccgcca ttatttcagt tgatacattt gtattgtttt tctttaaacc
    11581 gaaaaacttt tccataaaaa gttccactcc ctgaattttt ttctgatgga aaactataat
    11641 aataaaaaat aagtaaaata gggcaaatcc atcgaacaat ttcaaattat atagacgttt
    11701 cattcgcttt tcaacgtttt tttaacaaaa aaagcgaaaa taatacttaa cgttcgtgtt
    11761 tagcttatgt ttagctattt atatagccat aactgtagtt tttgttagga atagctgatt
    11821 gctcttgtta attacctaaa atatgctaga ctaaaacaaa tgctagttga ggaaggaagg
    11881 acccttattg aataaaaaat taattgccat cgatctggac ggaactaccc tcaatcaaga
    11941 ttcgatgatt acatctaaaa caaaagagac tctaaaaaaa gcaatcaatg ccggtcatca
    12001 tgtcagtatt gctacaggac gcccattccg aatgagccat cagttttacc aacaattaga
    12061 attaacaaca ccgatggtta attttaacgg cgcattagta cacatcccta atcagcactg
    12121 ggatggtgaa agagaaacgt tgatcaatcg tgaaatcgct tttgaaatct tatcgcaaaa
    12181 aaagcagcta aatttagatt ttatcgctgc tgaaaatcgt gatacttttt ttattgacag
    12241 ttttgatttc tttgatgaaa aaatttttgc ttcttctcgt ccaggagaga agaatttgct
    12301 ttctcctaaa aatttgacga caaatcctac atctttgctt gtccgaacag acaaacgttt
    12361 tgcaggagct gtatctgctg aattaacacg tcaatttggc agttatgtcg atgttcggac
    12421 atggggtgga cctactgcca tcttagagat tgtttcaaaa ggaatccaaa aagcaaaagg
    12481 cgtgcaagag attgctaact acctgtctat cgatcaaaaa gacgtcattg cctttggtga
    12541 cgagcataat gatttggaat tgttggatta tgcaggttgg ggagtagcta tggctaacgg
    12601 tactgaccaa ctcaaaggca ttgcaaatga tgtcactcct ttgagcaatc aggaagacgg
    12661 tttagctgtt tatctggaaa aactacttaa actataatag agaattagat tccacaaaaa
    12721 agaggttgtg acaatttttg tcacaacctt ttttgccgag taaacggtgt tcaaaagcca
    12781 gtttctcggt atgaagtcaa atttaagcaa aaatgtgaga agctattttc acaaaatttt
    12841 tcttcatact cgaaactaag cggctttttc acgacctctt ttttattttg ccatgaacat
    12901 atcataatac gcgtccatta tttctttagc aatcgtcaag tttatgtggt cttgttcgtc
    12961 taataagttc ggcaagacaa cactgatggc aatttccgga ttgtctgttg gtccgtatgc
    13021 cacgatatta ctattgactg tcgcaatatc tggatgacct tccgcaaccg tttctgcagt
    13081 ccctgttttt gcggaaaggt ccattttcgc actggctaat ggtttggctg ttgtgtaagg
    13141 atcggtacca tggacaactt ggtggaatcc ttcacggagg atcgccatat tttctgcaga
    13201 aatattgacc gtattttcta ctgttgtgcc aatttctttc ttcacttcac ccaagtttcc
    13261 attttcgtca ttgccataga tcgctttgac taggtgcggc tgtactcgtt tcccgccatt
    13321 agcgactgtt gaagcatatt gagctaattg gattggtgta tatgtgtcaa actgtccaaa
    13381 tgccaaatcg gtaaagttac ctggagcata ccattctttg ccattatccg cattgaactt
    13441 cttcatataa tcaacagaac gtgagatccc aggagattca ttcggcaaat cgatcccagt
    13501 cgttgtacca agaccaaact ctttgaaaga gtctctcaat tgctgataag ctttagcttc
    13561 ttcatctaaa ctaggtagtc ccattccagg tgtatagtct agtcctaaca tctttaacgc
    13621 cactttgatc atatacgtat tagaagacaa ttccgatgct ttgactgcat caagagcaac
    13681 ttgtccgctt cgattaaaga cagagctttt ttctgaagca ccttgcagac gaatcggctc
    13741 atcaatcaaa acttgatttc cgctgatcgc gttacttgcc cagcctgctg tcaaagtacc
    13801 agccttgact acagaccctg gatcaaaggc gctagtaatc gtgcctaaag cattctcggt
    13861 gatttctttg gaaccttgtt catgactgaa accagtcatt gctagcactt ctcctgtctg
    13921 aggattcata gccactgcat aagcaccagg cgaatactgt gctttgccgt tgttgatcaa
    13981 tgtttgataa ttccttttca aaatttcttc tacttttgat tggaattctg cattcataga
    14041 taacattaaa ttagaacctt tttccccaga gaagatttct ttttgattcg aaacatttcc
    14101 ttcattatca agggaaactt catattgggt tttggttcct tgcaggacat cttcatattg
    14161 tttttccaaa taactttgtc ctacacggtc attccgagaa taaccttttg ctaggtattt
    14221 ctctgcttca tctgctggaa gtccttgctt ttcagtcgtc acactgccaa gaatactttt
    14281 cagtgaactt gctgcattat attctcgtgt ccagtctgtg ccagtcgaaa ctcctggaag
    14341 ttcagaagcg cgttctgcca caacagctaa ctcttgatca gtaacaccag agtttttgat
    14401 atagacagtg tttaattcat atgcactatt catccgttta aagatggtag caatttttaa
    14461 ttgctcctcg tttaaattaa tctcatcagc tgtgacttta ttgaccatcg cactatattg
    14521 ttcgctgttc cctaattgct tttcttttgc cgaaagtctt tttgtggctt ctttcaaatg
    14581 tttgggatca gccagccaaa aatctttttt atctctatct gtcagatttt catctaccgg
    14641 aacatcgatc agcgagttca gcttcgttgc gatttcaagc aaatctttag ccgtaacttg
    14701 tgttcctctc gtatatgtga tggcctgatt cgctttattc gtaactaaag cttttcctgt
    14761 ggagtcgtag atcattccgc gaggagtact ttcttggacc gtaatcgtag aagtagactt
    14821 cactcgcgcc tccatctctt cgccgttcac gatctgcaaa taacccaatc tagcaatcaa
    14881 cgcaacaaat aacgtaaata taatgaaaaa aagaaaattt aagcgaaaag gaacatggga
    14941 ccggcgaaac gttttgttct taggttcttg tgcacctttt cttatttttt ctaaccaaga
    15001 agtatttaat atttttttca taaaatcttt catcatttcc tatccttccg cctatttaca
    15061 ttcatgcttc attgtaccca aaaacttctt aataaacaag aagtatttaa tatttttttc
    15121 ataaaatctt tcatcatttc ctatccttcc gcctatttac attcatgctt cattgtaccc
    15181 aaaaacttct taataaaaaa gaaaaaatgg gcggagcaag aaattcttta cctaatttca
    15241 agataaaatc aactttttgt ttcacagcaa tcacttaaag ttacatatcc aactattttt
    15301 ttcattaaaa aataaactta tttttgaaaa ttatgttttc ttaagtcgtg aaacactttg
    15361 atggatcatt tatttgtgga aaactacgtt ttgttattct aaaaaactta tataaaaaat
    15421 agtaaggttt ttatttcgaa tccataaaat attcttggat tgcttctcgt gtttttttca
    15481 ctttttcgct aagcttttcg tttttagcca tatagaaata aaaaatgata tcgatcgtag
    15541 caaattgggc caaaagtgaa ttagttgccg cacttcgatt cgtgatttct tttggtctag
    15601 atgtttgtac gagtacatcg gctatcttgc taagcgggtt atttccaaat tgcgtcaacg
    15661 caatgatttc gatattcatg cttttcgcca gctcggctaa tgcgactaca tctgcacttc
    15721 tacctgagtt tgaaacaagc caaagcactt tctttttctc attgctgaca agttgcggga
    15781 gcaagacgtg gatatctttt tcaaaaatga ttggcttacc gacacgagac catttttgca
    15841 gaatatcttc tactactaaa gaagaggcgc caaccccata taaaaaaaca gtctctgccg
    15901 attccaaaca ctctacgact ttttggatcg tttcttcttc tagaatatct actgtttctt
    15961 ttatcgctac ttgtgcattg ttaccaagtt tgtttttgat agagtaaaag gactcatttg
    16021 cttctatatc actaaaacct tcatcgattc cagtctgtag ttctgttgaa atcgtgattt
    16081 tgaagtccgt cagactttga aaaccgattt ttttggaaaa acgtacaact gtcggcgcac
    16141 tcaacccgga agcttctgct aattcattag ctgtcattgt agggaccttc tcaatattac
    16201 cgataatgta gttggccaac tttttttctg ctgaagaata atcttccatc atactttcaa
    16261 tacgactaat tacgctcata agttcctcca cccttttaat gatagtctat gtaagtatac
    16321 catatcagac ccttattcac ggtcgtatac tggataaaaa acaaaaaaac aatccgccca
    16381 acagaatatc ttatcccatc aatcccacag attctattga gcatctgtgt ctttgaggaa
    16441 tacctgttat ctattgttcg gattgctttc tatctactac ttttttacag gaaatttacc
    16501 tattagtttt ctctgctgta atttttgact tctgcttcca tagcatcaat aaagatattc
    16561 aaatcttcta cagctgtttc tttacttcca taacgacgta cgttcactgt tgcatcttcc
    16621 aattccttgt ctccaacaac taattggtaa gggatctttt gtgtttgaga ggcacgtatc
    16681 ttatagccca ttttttcatt acgatcatca acttctacac gcaacccctt catttgcaaa
    16741 cgttctttga tttcataagc ataatcgcca tgtgcatcaa cagaaacagg gatgattgtt
    16801 gcttgtatag gtgccaacca tgttggaaaa gcgcctttgt atacttctgt taaataagcg
    16861 acaaaacgtt ccattgtaga aacaattccg cggtggataa ctactggacg atgcgtattt
    16921 tccccgtctt ctccaacata tgtcaaatca aagcgctctg gcaataagaa gtctaattgt
    16981 atagttgata atgtttcttc tgtaccaaga gctgttttga cttgaatatc tagttttggt
    17041 ccgtaaaagg ctgcctcgcc ttctgcttca aagtaatcta gttctaattc atccatagcg
    17101 gctttcagca ttgcttgggc tttttcccac attgcgtcat catcaaaata tttatctgta
    17161 ttgtttggat cgcgatagct caagcggaaa cgataatctg taatattgaa gtcagcatat
    17221 actgccacca tcaattcaag cgtacgttta aattcatctt tgatttgatc aggacgaaca
    17281 aatgtatgac catcattcaa cgtcatttct cgtacacgtt gtaaacctga caaagcacct
    17341 gatttttcat agcgatgcat cattcctaat tcagcgatac gaattggtaa ttcacgataa
    17401 gaatggatcg tgtttttgta gaccatcatg tgatgagggc agttcattgg gcgaagaaca
    17461 agcatttcgc catctcccat atccattggc gggaacatgt cttcttgata atgatcccag
    17521 tgtccagatg ttttgtacaa ctcaacatct cccattattg gtgtatatac atgttggtaa
    17581 cctagactaa cttctttgtc tacgatataa cgttcgatcg tgcgtcggat tgttgcccct
    17641 tttggaagcc agaatggcaa accagaacct acttctgggg aaaccatgaa caagtcaagt
    17701 tcttttccta gtttacgatg gtctctttct tttgcttctt cacggagacg gatatattct
    17761 ttcaaagctt tcttgtcaaa aaaggctgtc ccgtatacac gttgcatcat ttgattgttt
    17821 gagtttccac gccaataagc acctgccact gataataatt taaatacttg tatacggcca
    17881 gtggatggaa catgtggtcc acgacacaaa tcgatgaatt cgccttgttg ataagcagta
    17941 atcacttctt cttctggcaa ttcactaatc agttctactt tgtacggatc acttgcaaaa
    18001 atttctaatg cttcctgttt tgaaagaaca cggcgttcga tcggcagatt ttctttgacg
    18061 attttcatca tttctgcttc gattgccggc aaatcttctg cagttacttg gttttgtcca
    18121 ttatctgtat cgtagtaaaa ccctgaatcg atagctggtc caacgccaaa gtggatattt
    18181 ggatagaggc gacgcatggc ttgggccatc aaatgagctg ctgaatgacg gactaagcca
    18241 agtgcatctt catggtctgg tgtgatgatc tcaatacttg catcttcctc gattggacga
    18301 gtcaaatcaa cgagttttcc gttcacttta ccagctaaag ctttttttgc taagcttttt
    18361 gaaatgcttt cagcaattgc caatgttgtt gttccagact caaattcttt gactgcacca
    18421 tctggaaaag taatttttat cataatagtt tcctccttga aatgttttga ttgatcagat
    18481 catcaagctg aaaaacaaaa aaatcctagc atctcttgaa aagagatact aggacgaaaa
    18541 gttcgtggtt ccacctaatg ttcaaacgca aatgcgttcc tcaagcgttg taacggtccg
    18601 accgcccttg cttcaacaag ggtttcgaaa gtggtcatcc tttggttttc tcctggaaaa
    18661 gtttcagcct agcttttcct ctcttatcga agactctcaa attcagttgg ctttctcatc
    18721 aatcaattat gtgtttattt aaccactaat aaatagaaaa atcaagtggg aaactaaact
    18781 tattttttcc attccccatt tacaccgtat gttccttttt tcatctcatt gatgatgaca
    18841 tggatgtgtt cttttggtgc ctgcgtgttt ttatgaacag cttccgtgat atctttcatc
    18901 atattttcta attgttccgg actgcgacct tctactaatt cgacatgtac gaatggcata
    18961 atgctctctc ctctttctga tcgttctttt ttcaactcga ttctatcata ctatgtttgt
    19021 cttgggtact gaatcaggaa aaatggctgg atcttttttt gctctcaagg cactcaaaat
    19081 agagaccgta tccacgactt cttgcaaaac agctccgaat aatgcgggga ttagtcccgt
    19141 acttgcaatt aacatcaaaa aaatacaaat aaagatgcca atcagtacag actgtttcgc
    19201 tattctcatc gtatctcttg aaatcactac tgcttttgct acacgcaaga gatcatcttt
    19261 caagatgacc gcatctgcac tttcactagc agctgtagaa ccatgagccc ccattgcaat
    19321 accgatatct gcagcagcaa gtgctggtgc gtcattcacg ccgtctccta ccatcgcaac
    19381 aggtctttga tctttaggga tactttctaa aatggtcagt ttatcttgag gcaagctacg
    19441 ggcatgtaca gaatctaatt ggactttttc tgcaatcgtt tgagcaatgg cagatccatc
    19501 cccggtgatc atcatcaaat ttttcacatc aagttttctt agctccttga ttgtctttgc
    19561 tgcttctggg cgaatctgat ccgaaaatag gatcgttcct gcaaaagatc gatcaattgt
    19621 tacatagatt gctgtattgg acgtttctat ttcttcagct gaaacaaaag aggcacgtcc
    19681 aatctttact tcatgattat tgactactgc ttggattcct tgaccggtca cttctttcag
    19741 ttgactaaca ggatacagtt ggctgttttt cacgttgttc actaacgaac gagctaagat
    19801 atggctagat tcctgttctg cactagctgc atacagaagt agctcttctt tttttattcc
    19861 attgactggc tgaatcgcct ctacttccaa tatccctttg gtaagcgttc ctgttttatc
    19921 aaaagcaatc gtttttgtac gtgcgagctt ttcaatcgtt gttccagttt ttacaacgat
    19981 cccgttttta cttgaacggc tcattcctgc aactaaagca atgggcgcag ctagaatcag
    20041 tggacaaggt gatgccacta ccagtacctc agcaaatcgt aaagggtctt ttgagaacca
    20101 ccaagaaata ccaccgatca aataagcaat caacgtgaaa gggactgcat aacgatcagc
    20161 taaacggaca aatcgggcag gcttttcttt tgattcttcg actagtttaa taatcatttg
    20221 atactggctg tctgcggcac gtttttcagc gatcatttgg atcgatccgt ctccatttaa
    20281 agaccctgac ataatctgat ctccaggttt cttggtttcc gggcgggact cacctgtcaa
    20341 agaagattca tcgaccgaag attctccttt ggtcactcga ccatctgcgg gtacaagttc
    20401 gccaggcttg actagtaact ggtcatcaat atttatttca tcgattacta tatcctgcaa
    20461 ttttccttct ttgaatcgat gggcaattct tggggtgtta tctaacaaag actgcagttc
    20521 tttacccgct cgtttattcg cgtaatcctc tagactatct ccgcccgtaa gcatgatcaa
    20581 tatcatcaaa ctagcccagt attctttgac ggctaatgtt gcaaggattg cggtaatcgc
    20641 taatacatct acgccatatc ttcctgaacg taacgtcttg atcatttcca aaaacatcaa
    20701 gaaggcgaga atccctccag taaatgcgac aatcgcaaat gcaagggccg gctggtgaaa
    20761 aacaaattca cttaataaag ctatcccacc aataagcaaa gtaagtaaaa attttttgaa
    20821 atgtgtcatt gttttttctc ctcttttatg tgactt
//
LOCUS       DAFEPQ010000046        20285 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.048, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000046
VERSION     DAFEPQ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.048"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            140..460
                     /locus_tag="LP543_001797"
     CDS             140..460
                     /locus_tag="LP543_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321963.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="LP543_001798"
     CDS             912..1112
                     /locus_tag="LP543_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL2321964.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="LP543_001799"
     CDS             1259..4048
                     /locus_tag="LP543_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBL2321965.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="LP543_001800"
     CDS             4095..4622
                     /locus_tag="LP543_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HBL2321966.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="LP543_001801"
     CDS             4643..5833
                     /locus_tag="LP543_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL2321967.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="LP543_001802"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="LP543_001802"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBL2321968.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8224..8898
                     /locus_tag="LP543_001803"
     CDS             8224..8898
                     /locus_tag="LP543_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL2321969.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8895..9236
                     /locus_tag="LP543_001804"
     CDS             8895..9236
                     /locus_tag="LP543_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321970.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9266..9625
                     /locus_tag="LP543_001805"
     CDS             9266..9625
                     /locus_tag="LP543_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBL2321971.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            10149..12230
                     /gene="cadA"
                     /locus_tag="LP543_001806"
     CDS             10149..12230
                     /gene="cadA"
                     /locus_tag="LP543_001806"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBL2321972.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            12541..14007
                     /locus_tag="LP543_001807"
     CDS             12541..14007
                     /locus_tag="LP543_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2321973.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            14622..15161
                     /locus_tag="LP543_001808"
     CDS             14622..15161
                     /locus_tag="LP543_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HBL2321974.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            15235..15675
                     /locus_tag="LP543_001809"
     CDS             15235..15675
                     /locus_tag="LP543_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBL2321975.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            15688..15897
                     /gene="copZ"
                     /locus_tag="LP543_001810"
     CDS             15688..15897
                     /gene="copZ"
                     /locus_tag="LP543_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBL2321976.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            15897..18083
                     /locus_tag="LP543_001811"
     CDS             15897..18083
                     /locus_tag="LP543_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBL2321977.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            18103..20274
                     /gene="copB"
                     /locus_tag="LP543_001812"
     CDS             18103..20274
                     /gene="copB"
                     /locus_tag="LP543_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HBL2321978.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
BASE COUNT         6776 a         3527 c         4407 g         5575 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaacggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ctatcatcat
     5341 caacggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa
     5401 tcctgtgatc atcaatggaa tgatcgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag caagatacag tggaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatattcg cttgtggcag aactgccggg
     5581 tgtttctctt gaaaagccac aaggagcttt ttatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctaaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatat gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttatgatga
     6721 tgcgatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtgat
     6901 ctgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacagactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggtgatg aaaaagctgt tctgcatcaa
     7201 gtaattagaa cagccaagca aaaacagctg ttcatgtttt tgaattggcg ggacttatta
     7261 ccgcagatgt aagcttttga ataccgttta gtggagaggt tgtgaaaaag ctgtttagtt
     7321 tcgagtatta agaaaaattt tgtggaaatg gctttccaca tttttgcata aatttggctt
     7381 aataccgaga aactggcttt tgaacaccgt ttaaggaaga ggttgtgcgc ctgccgttca
     7441 gctccgagtc acaaggagta atttcaaaaa acagtttttc atgtttgatg actattgtga
     7501 cttgtgaccg aggagttggc cggagagcac cgtttattct aaaaaagaac tgcgaactct
     7561 cgcagttctt tttttaaaat acgggataaa ggaagtttcc agcagacaag agaagaaagc
     7621 aaaagaaatt gctatttgat tcacaaggtt ctataacgaa atcgcaatca atcaaacatt
     7681 tacaaagaag gtaattcaaa tggaaatctg atttgatgat gcttctgctg ttgaaataaa
     7741 gcagcatcta gcacctgaaa acaaactaat agtaacattc gaagatggag taggtccgta
     7801 ttttcagcat gcaataatcc atatgtaagt tcaattcacc cttgaaatca tcggaccaga
     7861 taaagttatg accaatctat cccaagcaat ctagaagaga tattagtaaa gggacattta
     7921 ctagaagtct tagatgagcc tataaatata tgttctgatg gcgtcatcag tggtctgatt
     7981 gacggaagct taggtgtgat cgatctctct ggaccaaaca acgaaggaat caaggggaat
     8041 ccggcacgtt aagaaaaaaa tagcataaaa aactagacac ttgatgagcg ttcattcaat
     8101 tgtttgaagg gactaggctg gtgtcatttt ttatacatat attttctctt tcgtcttgat
     8161 agggcttatg ggataatata aggaagaaaa tcatcgtttt attcaaaaag gagtttttga
     8221 caaatgaaaa aacgtatatt atttatcggt gatagcatta cagacgcagg aagagaccga
     8281 gcagaagcaa ctgatctagg taaaggttat ccacttttga ttgccggaaa actaatctcc
     8341 caatatccaa cgatggaatt tgaatgcatg aaccgtggga taggaggaga caaaattgca
     8401 gatctgcaag tacgctggca agaagattgt ttagctttac aacctgacta tgtatcgatt
     8461 ttgatcggaa tcaatgatac ctggcataat actagcgatc aggctatatt cggaactgag
     8521 aaatctgctc agcaatttga atccgtttat cgaaaattac taaatgaact tcaaacagtg
     8581 ggaatctgca acatcatctt gatggagccg tttgtacttc ctataaccga cgaccgtcat
     8641 ggctggcgta aagacttaga ccctaaaatc caaattgttc gtaaacttgc taaggaatat
     8701 ggcgcattgc tggttccttt agatggtata ttcaatgctt tagggattgt ttatggtttt
     8761 tcgaaatata caagagaaga tggggtccac ccaacgattg gcggacatga gatcattgca
     8821 caaagctgga tagatgcaat tgccgcagtc gattttttta gtggagataa aaatgagcga
     8881 aagaaaagtg agaaatgatg gaaatacggt gggcagtaaa agaagatcta cctcaaatca
     8941 atcagctgat tactgatcat ccagaatcat tcgtaaaagc aaaaacggca gctgacctaa
     9001 atgataagaa taaactattg ctcgttgctg taacaaaaga aaaacggatt atcggatttg
     9061 cgcaagcgga cgtgcaacag tcagaagaac gattggatca gcgaccatca ggaaaactaa
     9121 tccaacacac attagtaaca aaagaaaata cggaagaaat ccaaaaaaaa tttgcagaat
     9181 atttgatact ggaagctaaa aaaagaagaa taaaatcatt ttttgcccag ttttaatcaa
     9241 aaacagtaat agaaggagaa atacaatgag ttgtttagga aatgtcatct ggtttatatt
     9301 cggcggacta atcggtggtc ttagctggtt agcagcgggg atactttggt gtatcacgat
     9361 cataggtatc ccaatcggta tgcaatgttt taagatcgcc tccctttctt ttgcgccttt
     9421 tggcaaagaa gtaatttatc accagtctgg catgtcattg attgttaata tcatctggct
     9481 gatcgtttca ggcctaccaa ttgctctcgc acatgtcgtc agcggcatcc ttctatgtat
     9541 cacgatcata gggatacctt ttgccaagca atctttcaaa cttgctcgat tggcgttgat
     9601 gccattcggt gcagaaattg tataaaagag aataataaag gttagataaa gcatagagtc
     9661 aggtgggata catgtatttc acctgactct atgcgttctc ctgtataagt tcttccatgt
     9721 gacttttacg tcttttattt atatggggtt actgtttatg gaaagttgaa ataattttca
     9781 ttaaagaact ctttctatat ttttgaaagc gtagtggagc ttcgaatatc tgggaaggac
     9841 attttgcagg gattgaaaaa agatattgtc ctatcgaaaa attaatgaat ttagacaaat
     9901 tgccagcaac aggcttcaaa cttagttgct atcctgtcaa gatcaagaag gcaagtgcgg
     9961 gttggataag agcagtggca atgatcgaaa agaaaaagaa agaataagcc aaaacatctg
    10021 tatggaagaa attttccgat gcagatgttt ttcatttttt ctttcggata ccttgacata
    10081 tgaataaccg ttcatttata attagttatg aagagatatt catatgttga ggcgaggagg
    10141 tcattaaaat gaaaagttac agattagaag gtttagattg tgcgaactgt gcaatgaaaa
    10201 tcgaaaaagg agttcagaag atcaatggag tcaaggaagc aacggtaaac ttcacttctg
    10261 gaaaactaac gattgacgca gaggaagatc atttggctac gattgaacaa gaaataaaaa
    10321 aagtagtgaa ggaattagaa ccagatgtca aagtaacaga aatagataag gaaaaagtat
    10381 ctgaacatgg aaatgaaaaa gagagaaata cgctgttccg gattttattt tcccttgcag
    10441 gtatcgcttt attgctatta tttgatttta atgaaccaat tcgcttgata gggtatttat
    10501 tgatttatct gctgatagga tatgatgtag tgaagaaggc agtgatgaat atcgtcaaag
    10561 gaaaaatctt tgatgagaat tttcttatgt ctgttgctac gattggcgct atgttgatcg
    10621 gtgaatatcc agaagccgta gcagtgatgc tattttatca aatcggcgag tatttccaag
    10681 gattggcagt cagtcattca agaaaatcga ttcgcgaatt gatggcgatc cgtccagaaa
    10741 cagctcacgt gcagacgacg gaaggattga tgaccgtgaa ccctgaagac gtgcagatcg
    10801 gtcagtttgt acttgtgaag cctggtgaac gtgtaccgtt agatggaacg atcatcgaag
    10861 gagaatcatt agtcgatacg tcagcactta caggagaatc agtccctaaa tctgtatatg
    10921 ttggtgaaac agtattaagt ggatttatca ataaaaataa accattttta gttcaagtag
    10981 aaaaatctta tgaaaattct actatcagca aattattaga attagtagaa aatgccagca
    11041 gtaaaaaagc accagctgaa aatttcatta ctaaattcgc gcgctattac acaccagtcg
    11101 ttgtaggatt ggccgtttta ttagctgttt tacctccact tgttgtttct ggagcggcgt
    11161 tttctgaatg gatctatcga gcattgacat ttttagtgat ctcatgtcca tgtgcattag
    11221 tgatctccgt accccttagt ttctttggcg gtatcggagg agcaagtaaa atcggtgtat
    11281 tagtaaaagg ttcaaattac ttggagttgc ttgcacaaac tgagacagtg gtattcgata
    11341 aaaccggcac attaacgaaa ggtgactttt cgatccagac gatcgataca aaaatcgatc
    11401 caaagatatt catgcaatat gttgcgagtg cagaacagtt ttctacacat ccgattgctc
    11461 aatctgtttt agaaggctac gcaggtccgc ttttaccaac agctaatatc caagaatttg
    11521 caggtgaagg gatcttagct gaagttgatg gcaaacaagt attagcaggt aatcacaaat
    11581 tgatggaaag atttgaaatc tcttttcctt caagtcaaga aatcggtaca ttgctttatc
    11641 tagcgatcga tcaaagttac agtggttatc ttgttattgc agataccttg aaagaagatg
    11701 ccgtagacgc attggttcaa ttaaaaaagg ctggtgtgaa gaacacagtc atgttgacgg
    11761 gcgattcgaa aaaaattgct gatcatatcg ggaagcaagt aggcgtggac aagatatata
    11821 gcgaattgct tccagaagat aaagtgcagc ggttagagga aatcttacaa agaaacaata
    11881 aaaagaccgc atttgtcgga gatggaatca acgatgcacc ggtgctagca agagcagatg
    11941 taggaatcgc aatgggtgga ttgggaagcg atgctgcaat tgaagcagca gatgtagtca
    12001 tcatgaatga tcaaccatcg aagattgcag aagcaatcca tttagcaaag aaaacattga
    12061 aaatcgtcaa acagaacatt gtctttgcta ttgggataaa aattttggta cttgccttag
    12121 gtgcttttgg ttttgcatca atgggagatg cagtatttgc agatgttggg gtgacagtat
    12181 tagctgtttt gaatgccatg cgaagcttgc atgtaaaaaa taaacgttga tcaatgaaga
    12241 ggacgaatga acgtcctctt ttttttatat ccttttctta atgaaaaatg taaagaaaat
    12301 agataggatt ataaaataac taatgttttt aattttgcag aaaattcaaa atagcttgaa
    12361 attcaataag gtaaatgaaa taaaagaaaa taacttattt ttttgatgga aaaaatatga
    12421 aaaaacgatg tgtattaaat aaaagaatac acacataaaa taacactgtc ttgtaagttt
    12481 gttaaaaatt gaatatgatt acatctggat aacaaatttt tatcataaag gagagaacga
    12541 atgaacaaca aacaattacg ctggtttact gttgcgttaa ttgcatttaa catggtttgg
    12601 ggcatgggga atgtagttaa taactatgct cagcaaggag tatcagttgt cacctcttgg
    12661 ctattgattt tggccattta ttttattcca tacgcattga tcgttggaca attagggtca
    12721 gccttcaagg atagtaaagg aggcgtcagt tcatgggtcg aaaatacgac atctaataaa
    12781 cgactggcat actatgccgc ttggacttac tgggtcgtcc atattcctta tttggcacaa
    12841 aagcctcaag caatcctgat tgctggcggt tgggcagtcc aaggaaatgg aaatatcgta
    12901 aatacgatgt cagtacaaat ggtagcagtt atttcactta ttatatttct tgccttttta
    12961 tatttatcaa caaaaggact ttctactttg aaagtgattg gcggactggc cggaacagcg
    13021 atgttcgtca tgtcgatctt atttattctt ttagctgtaa cggcaccgtt catcaatcca
    13081 acgatggaat ttgccacacc acatatgaat caagtgaaaa catatattcc aacatttgat
    13141 ttcagttatt tcacaaccgt ttcgatgcta gtatttgcag ttggaggagc ggaaaaaata
    13201 tccccttatg tcaactctac aagaaatcct gcaaaggaat ttcctaaagg gatgatcttc
    13261 ttagcagcaa tggtagggat ttgtgctgta ttaggctcag tcgcaatggg tatgctgttt
    13321 gctagcgata atattcctaa agacttgatg gcaaatgggg catacagtgc ttttcaaatt
    13381 ttaggtaatt actatggtat cggtaactca ttgatgatca tttacgcatt gacaaacatg
    13441 attggacaaa tctccgcatt agccttttca attgatgcac cactaaaaat cttgttagca
    13501 gatgcggatc cagaatttgt accaaactgg ctacgtaaac gtaatcgtaa aggtacattg
    13561 gtgaatggtt atcttctgac aggtatacta gtaggaatca ttattttgtt gccgatcttt
    13621 ggtatcaaag atatgaatga attagtaaaa tggttgacga acttgaactc agttgtcatg
    13681 ccaatgcgtt acctatgggt gttcttggca tatatgatgt tgaataaagc ttataagaaa
    13741 ttcaattctg aatataaatt tgttaaaaat ccaaaaatcg gttttatctt tggtgcttgg
    13801 tgctttgcct tcactgcgtt tgcatgtatc ctaggaatgg tgccaaaagt agattatact
    13861 gctgatccta aagcatggtg gttccaatta ttcacgaata tcatcacacc aattgtcttg
    13921 atcttattag gtatgatctt gccagcaatt gctcgtcgag acaaaaacaa agaaatcact
    13981 gaaccagaaa cagtggttca gccttaaaat agaaatactt gttacaagag gttgtgacag
    14041 aagtggtcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    14101 aagtttcggc ttgtttttcg cagaagctac ttctggaaca ccgtgaatca gaggttgtga
    14161 aaaagctgtc ctgcatcaag taataagaac agccaaacaa aaatagcccc tcatattttt
    14221 cgttttgctg ggcttattac cgcagatgca agcttttgaa caccgtttaa gaaagaggta
    14281 gtgagcctgc cgtttaactc cgagtcacaa ggagcaattt caaaaaacaa cttctcatgt
    14341 ttgttgacta ttgtgccttg tgaccgaggt gttggcagga gaacaccgtt taaggaagag
    14401 gttgtaaaat cagcgatttg acctgagtat tagaggaaca aacgaaaaaa tagctcctca
    14461 tattttgccg ttttttgagt taatgctgaa ggtcagctga ttgaacacca tttattcgaa
    14521 acaagggact ttgacacgac ttttgtcaca gtcccttgtt tttttgcggc gttataagtg
    14581 gtatgatcga accaaattcc aaagagaagt gagggagaag aatgaagatc atcgtgattg
    14641 gacaaagtgg gtcgggaaaa tcgaccctcg caaggaagat caaagaaatc accgactttc
    14701 cgctactgcc gctagacctt ttatggcata caacagacta cagtattcag gcaaaaaagt
    14761 ggtttttgca agaacagcaa tcgtttatgg tatctaatcc gtcatggatt gttgaaggaa
    14821 attatacttc tacattagag gaaaggatta aagaagcaga taagatcatt tggcttaaag
    14881 tgccgcgtta tctcgccatc taccgagtag tttggcgttc tctaaaaaga aagataaaca
    14941 aaaaatctcg gccagatatg cctgaatcat tttcagagaa actaaacagg gaatacttag
    15001 aatttttatc ttttatctgg cattttgaag aaaacaatga accgaaaata cagcagctga
    15061 tccaagtagc taatgcaaga aataaactga tcatccttaa aacaagacga gagaaacaag
    15121 aatttttaac taaattgaaa atagagagca aaaataattg acagtttttg tttacaagcg
    15181 taatctgtat tcataagcta atgattacat ttgtaaacga aaggtgtgaa ataaatggaa
    15241 aaagtcaaaa cgccagtaaa gatcagtgat tcagagtggg agatcatgcg tgttatttgg
    15301 acacttggac agacgacagc ccaagaaatc acgcagatct tagctgattc gatggattgg
    15361 aaaccagcaa cgatcaaaac tttgctggga cgtttggtaa aaaaagaagt gatctggaca
    15421 gaacaagaag gaaaaaaatt catttatcat ccagctgtct cggagatgga aacagttcgc
    15481 agtgcgacgg aaaatctttt ttcccatatc tgtgcaaaaa ggattggaga gacgattgcg
    15541 gatctagtag aagaagccac tttgactcaa gaagatatca atgagatttt gaaacaactg
    15601 gaacaaaagc aaccagtaga tacgatcgaa tgtaactgta tcccaggtca atgtgaatgt
    15661 aaacaacatc aataggagga agaaaaaatg aaacaacaat tttcaatcga aggtatgagc
    15721 tgcaatcatt gtgcagcaag agtagaagaa acagtaagcg cacttgacgg cgtgcaaaaa
    15781 gtgaaagtaa atttgaaaaa agcaaacggt acggtcaagt ttgatgaaac aaaagttcaa
    15841 tccgaaaaga tttgtcaagc aatcaatgga ttgggttata aagcagaggt gatttgatgg
    15901 caacagcaga aaccaaaatg gatacgttcg tcattacagg tatgacttgt gcaaattgtt
    15961 ctgctaggat cgaaaaagag ctgaacggac aacctggtgt ggtcaatgcc acagtcaact
    16021 tggccactga aaaagcaagt gtcaaatatg aagggacgac cactgaaaaa ttaatccaaa
    16081 gcgtagaaaa catcggttat ggtgcaattt tgtacgatga agcccacaaa caaaaaattg
    16141 cagaagaaaa gcaagcgtat ttgaaaaaaa tgctattcga tcttatattg agtacggttt
    16201 tgactttgcc gttgatgttg tcaatgattg ctatgatgtt aggaagccat gcggcgattg
    16261 ttcacttttt tcattttcca atcgtccagt tagtgctgtc tgctcctgtc caattttatg
    16321 tcggtgctcg tttttataaa ggtgcgtacc atgcaatcaa gacgaaagca ccaaatatgg
    16381 atgtattggt agcaattgga acatcggctg cctttgcatt gagcatttac aacggatttt
    16441 tccgtggaca cccccaagat ctttattttg aaagcagcag tatgatcatc acgctgattt
    16501 tgctcggcaa atatttggaa catacagcaa aaacgaaaac aggtaatgca atcaaacaat
    16561 tgatgtctct tcaaacaaaa accgcccaag tgataagaaa cgggaaagaa gaaacgttag
    16621 cgattgaaga agtagtcgtt ggtgatcaac tagtcatccg tcctggtgag cagattccag
    16681 cagatggtcg aattatttca ggaagcagtg cgatagatga aagtatgctt actggtgaaa
    16741 atcttcctgt agaaaagaat ccggatgaca cgctttttgg cgggacaatc aatacgaatg
    16801 gtctgctaca catggaagtc acacaagtcg gcaagcagac agtattagcg caaatcattc
    16861 aaatggtgga agatgctcaa gggagcaaag ccccaatcca aaaaatcgca gatcggatct
    16921 cagggatttt tgttccgatc gtattagtca tcgcgtttat cacgctcata gctacaggtt
    16981 tgatcactgg tgattggcaa ctcgcattga tccatagtgt ctctgtatta gtcattgcgt
    17041 gtccatgtgc attaggttta gcgaccccaa cagctattat ggtaggaacg ggtgttggtg
    17101 cacgaaatgg aattttaatt aaaggcggag aagcattaga agcagctgct cacttagaca
    17161 gcattgtttt ggataaaact ggaacgatta cagaaggaaa gcccaaagtt acagatctag
    17221 tcggttcaaa agaagtccta tctatttttt atactttaga acaagcttct gaacatccac
    17281 taggtaaagc aatcgtggaa tacggaaaac tgcaagaagc agcaacatac gatatgattg
    17341 attttacagc ccacccaggt gctgggatca gcggtacgat taatggagta cgctattttg
    17401 cgggtacacg taaacgatta attgaattga acctatcatt tgatgaatac caagaacacg
    17461 cgttagaatt agaacagcag ggaaaaacag tcatgttttt agctgatgaa aagcaagtga
    17521 tcgggtggat tgccgtggct gatcaaatca aactagaagc caaacaagca atcaagcagc
    17581 tccagaataa aggattagat gtttttatgc tgacaggaga caacaaactg gcagccgaaa
    17641 cgattggtaa acaagttggt attgatccaa agcatatctt tgctgaagta cttccagagg
    17701 ataaagcagc ctacgtagaa aaacttcaga aagatggcaa aaaagtcggt atggcaggcg
    17761 atggaatcaa tgatgcaccg gccttagcat tagctgatgt cggtatggca atgggaagtg
    17821 gaacggacat tgcgatggaa acagccgatg tgacgctgat gaatagcagt ttggcatcca
    17881 ttgcacaaac aatcgagttg tctaggttga cattgagaaa aatcaaacaa aatcttttct
    17941 gggcttttgt ctataatacg attggtattc cttttgccgc actaggcttt ttgaatccaa
    18001 tcattgcagg aggagcgatg gcgtttagtt ccgtcagtgt attgttgaac tccctgagct
    18061 taaatcgaca catgaaaaag taagagaagg gttggagtaa aaatgaatgg gaacgatatg
    18121 gagaaaaaac atgagcataa aaaagaagta gtaaacaaag aaagtacaga atctcatgaa
    18181 aaacagatgg agatgaaaca tgaccatagt cagatggatc attccatgca catgggacat
    18241 aaccatggag atatagcaaa cagcaaacaa atggaccatt cgatgcacat ggatcacgaa
    18301 cacggcggca tggatcattc catgcacatg gggaatttca aacaaaaatt ttggctatcg
    18361 cttatcctag ctatcccaat cattcttttt tcaccaatga tgggaatgga atttccattt
    18421 caagtaacgt ttcctggttc tgattggctc gtattgatct tagcaactat actatttatc
    18481 tatggcggtc agccattttt aagtggagct aagatggaat tgaaacaaaa aagtcctgct
    18541 atgatgacac tgatcgcgat ggggattaca gttgcatata tttatagtgt ctattcgttt
    18601 atcgcaaatc tgctcaatcc acatacacac gtcatggatt tcttctggga attggctact
    18661 ttgatcgtta ttatgctact aggtcactgg attgaaatga atgctgtgtc gaatgccagc
    18721 aatgctttac aaaaactagc agaattactg ccagaatcag tgaaacgctt gacgaaagat
    18781 ggaaaagaag agacagtttc attgaaagaa gtaaacgaag gtgatcgttt gatcgtccgg
    18841 tctggtgata aaatgccaac agatggtgta atcttgaaag gtgaaacaat tgttgacgaa
    18901 tcagctgtga ctggcgaatc aagaggaatc aaaaaacaag ccaatgacaa agtgattggc
    18961 ggttctatca atggggatgg aacgatcgaa atcgaagtaa ctggaactgg tgaaaatggt
    19021 tatctagcga aggttatgga aatggtgcga aaagcacaag gagaaaaatc caagctagag
    19081 tcattatctg acaaagtggc aaaatggctg ttttatgtcg cattaattgt gggtatcctg
    19141 gcattcatcg cctggttgtt cttgacagat cttcctaatg cattagaacg tatggtaaca
    19201 gtctttatca tcgcctgtcc tcatgctcta ggtttagcta tcccattagt tgttgctcgc
    19261 tccacatcaa tagcagctaa aaacggatta cttttgaaaa accgcaatgc actggagcaa
    19321 gccaatgatc tagatgtgat catgctggac aaaacaggaa cactaacaga aggaaaattt
    19381 actgtaacgg gtgtcgaagt acttgatgat gcgttcaata aaaatgaaat ccttcaatat
    19441 ctaggagcct tggaagcgaa tgccaatcat ccattggctg ttggcattat gaattatttg
    19501 aaagaacatg aaatcaagcc ttatcaagct gaaaatctaa aaaatctttc aggagtcggg
    19561 ctagaggcaa cagtcaaaaa acaacaagtg aaaatagtta atgaaaaaga agttgagcgt
    19621 ctcggattaa acgtagaaca agcattattg aaaccttacc aagaacaagg aaataccatc
    19681 agcttcctga ttttagaaaa tcatctagct gcaatcgttg ctttaggtga cgtagtaaaa
    19741 acagaagcaa aagaatttat ccgaacattg aaagaaagaa aaatcactcc tgtcatgctt
    19801 actggtgaca ataaaaacgc tgcccaagca gtagcagatt atttagggat cgaagaatat
    19861 tatggcggtt tattaccaga tgataaagaa gctgttgtcc agaaatacct tgaccaaggg
    19921 aaaaaagtaa tcatggtggg tgatgggatc aatgatgcgc caagtttagc ccgtgcttca
    19981 atcgggatgg caatcggtgc agggacagat atcgcaatcg attctgcgga tgtcgtattg
    20041 acaaacagcg atccaaaaga tattttgcat tttcttgatc tagccaagca aacgcgaaag
    20101 aaaatgatcc agaacctctg gtggggggca ggttacaata ttcttgcaat tccgctagca
    20161 gccggaattc ttgcgccaat tggtatcata ttaagtccag cagttggtgc tgtcttgatg
    20221 tcgttaagta cggtagttgt agctctaaat gctttgacat tgaaaatcaa ataataatga
    20281 aaaaa
//
LOCUS       DAFEPQ010000047        19200 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.049, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000047
VERSION     DAFEPQ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.049"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            112..306
                     /locus_tag="LP543_001813"
     CDS             112..306
                     /locus_tag="LP543_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321979.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            575..874
                     /locus_tag="LP543_001814"
     CDS             575..874
                     /locus_tag="LP543_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2321980.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(971..1405)
                     /locus_tag="LP543_001815"
     CDS             complement(971..1405)
                     /locus_tag="LP543_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2321981.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1637..2347)
                     /locus_tag="LP543_001816"
     CDS             complement(1637..2347)
                     /locus_tag="LP543_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBL2321982.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2337..3197)
                     /locus_tag="LP543_001817"
     CDS             complement(2337..3197)
                     /locus_tag="LP543_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBL2321983.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3202..3840)
                     /locus_tag="LP543_001818"
     CDS             complement(3202..3840)
                     /locus_tag="LP543_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBL2321984.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3855..4154)
                     /locus_tag="LP543_001819"
     CDS             complement(3855..4154)
                     /locus_tag="LP543_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2321985.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4197..5681)
                     /locus_tag="LP543_001820"
     CDS             complement(4197..5681)
                     /locus_tag="LP543_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBL2321986.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5701..6162)
                     /locus_tag="LP543_001821"
     CDS             complement(5701..6162)
                     /locus_tag="LP543_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2321987.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="LP543_001822"
     CDS             complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="LP543_001822"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBL2321988.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7465..8235
                     /locus_tag="LP543_001823"
     CDS             7465..8235
                     /locus_tag="LP543_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL2321989.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8508..9335)
                     /locus_tag="LP543_001824"
     CDS             complement(8508..9335)
                     /locus_tag="LP543_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2321990.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9903..11138
                     /locus_tag="LP543_001825"
     CDS             9903..11138
                     /locus_tag="LP543_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBL2321991.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12137..12397)
                     /locus_tag="LP543_001826"
     CDS             complement(12137..12397)
                     /locus_tag="LP543_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBL2321992.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12424..13725)
                     /locus_tag="LP543_001827"
     CDS             complement(12424..13725)
                     /locus_tag="LP543_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2321993.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14017..14475)
                     /locus_tag="LP543_001828"
     CDS             complement(14017..14475)
                     /locus_tag="LP543_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL2321994.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14488..15819)
                     /locus_tag="LP543_001829"
     CDS             complement(14488..15819)
                     /locus_tag="LP543_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HBL2321995.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15830..17272)
                     /locus_tag="LP543_001830"
     CDS             complement(15830..17272)
                     /locus_tag="LP543_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2321996.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17259..18023)
                     /locus_tag="LP543_001831"
     CDS             complement(17259..18023)
                     /locus_tag="LP543_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBL2321997.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18127..19002)
                     /locus_tag="LP543_001832"
     CDS             complement(18127..19002)
                     /locus_tag="LP543_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL2321998.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         6102 a         3948 c         2949 g         6201 t
ORIGIN      
        1 aaaactttca aattgattct ttttatttat cataaagata tagatacata atcattttca
       61 cttacatata tttgaatatt aactaaaaac agcaagaaag aagaggatca aatgtattat
      121 gtagaagtaa aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat cagtaacgaa
      181 tatcctttac ttggttcatg gaaagaagct gctccttttt ccaaaccatg tgccataaaa
      241 ataaagaacg agctagaaaa agaactgact tgtggtaaag ctgttgtaga tatcatcgaa
      301 aagtaaatcc aatgtataga aaggaagatc acacatgaaa agcgacgtac aaattgctaa
      361 gattatagaa ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt
      421 ataccatatg attatttcaa tatgtatcct ggaattggag atctcgtaga gctatatcaa
      481 gatgaatcaa atatacttgt cattcatcat gaaatataga gacttataaa attgtttatt
      541 tttataatgt atcagtatag aaaggaaata aaacatggat tatactgttg aatttgacag
      601 tgaactactt aagcaatttc cacaagaaga acatttcatg tataaagaag caatttccca
      661 caaagtgaca gagtatttgg agcataattt ttttcgtata aagccagtgc gtactcgttc
      721 ggcaaaagga atatacgaga tgaaagtcaa aattaataaa aaaaattatc gcatagcatt
      781 tgctttaaaa aaaaatcatg tgttcatttt ttatattagt tcgaatttgc aaaaaattgt
      841 atttgataaa gaggtgagta aaaaaataaa atagctgagc attggaggat agattgagtg
      901 atactaagtc tatcctcttg tttttctaaa gaactaagct agaatttttt cttttgtacg
      961 ttattatatg ttatttgtca ttaccaaaaa gcaccttttt tcttctagta tctatgacat
     1021 aagaaatacc acccatttcc tttacttgaa caaatcctac tgattcataa aatttttttg
     1081 ttttctcagt atcatctgtc aataattcga tttggactgg tccataacta gtgatcttct
     1141 ccaaagcttc attcaaaagc actttgccta ttccttggcg ctgatacctc ggatgaatga
     1201 ggatatcttg aataaatgcg atcgtcgcaa aatcagtgat ccaacgaacg attccgataa
     1261 tcttcccttc cccattacgc tttatcagag attcagattg ttcaaatgct ctagctaacc
     1321 ttgcagtgtc ttttgtgtaa ttcgtccaac caacgctctt atataattca gaaatctcat
     1381 cgatcataag ggaatcaata ttcatttttt tcttcctttc ttatacaatt ctccctctaa
     1441 taatacagtt ctttgactat ttaatctatc acatataact ctcctgtaaa gattctctcg
     1501 attccttaaa attcctcttt ccttttagaa tgcatacttt ttgataaata aaaaggttgt
     1561 gataaatttt cttcacaacc tctgtttctc tctatcaagt gttatctttt aattttctac
     1621 ccttacccta gcatctctat tgcccgtagt atgcatactt tccatgtttg cgaaaataat
     1681 gtttatctag taaatattgc gggatttcac atatattggg gttgatcagc tcgttttctt
     1741 tggccatcag acaaatttca tctaagatca aactgttttc tactgctttc ataggtgaat
     1801 ccccccatgt gaacggtcca tgtccataga caagtactcc agggacttcg tttggatcta
     1861 attttctttc cttaaacgtc tcgacaatca cattccctgt atgaacttcg taagcttctc
     1921 ttacttcttc tttcgttaat tggcgtgtac aaggaacctt accataaaaa gcatcagcat
     1981 gagtcgtgcc ataagctggc aaatcgcgtc ctgcttgcgc ccacatgacg gagtgcgtgg
     2041 aatgagtatg agtaatagca gttatatctt caaaagtttg atacaaaaca acatgtgtgg
     2101 ggagatctga agatggtttt aagctatctt cttctattat atttcccgac aaatctgtta
     2161 ccaccatttg atctgcttgc atgcattcat atctgacacc gcttggctta atgacgatga
     2221 ctcctagctc acggtccact tcactcacat tgccccaagt caatttgact aatccgtatc
     2281 gcggcaatgc cagatttgct tcaaggacac gttgcttcat tgctgcaatt ttttcttcac
     2341 aagacatcat agccagcctc cttcaattta gaacttaagt agtcttttgc ctgctggatt
     2401 tctcttcgaa agtcttggct tttttcactc cacatttcaa tcagaaatgt tccatcataa
     2461 tctagacgtt tcaaatgtct taataatcca agaaaatcaa cacaaccgct accaaaagga
     2521 acatcacgaa actggcctga atattctttt gttacagggt aagtatcttt taagtggata
     2581 gcagtgatat aatcgatccc tttctcaagt tctcgagccg gattattttc cggccatgcg
     2641 gacaaattgc ctaggtctgg atatacttgt agaaaaggtg aatgaatctg ttctttgatt
     2701 tctaaaaatt ttgaaatgga gttcataaat ggatcatcca tgatttccac ggaaagaatg
     2761 atgccatatt tagaagcttc ctttacgctt tcttttattc cttcgataaa gtcttctctg
     2821 gaattcattg atttctcttc ataataaaca tcgtatccag ctatttggat tactttgacg
     2881 cttagattat gcgccaaatg gattgctttt ttcattagtt gtttcgctgc ttttttcttt
     2941 tctggatcag cagaaccaaa cggaaatcgc cgatgcgcac ttaaacaaat cgaatggata
     3001 cgtacaccgg agctgaacat ctcttctctc agctcagcta tcttttcttc agaccaatct
     3061 aatcgtgcta accgctcatc cgtctcatca atcgacatct cgataaaatc gaagtccatt
     3121 tcttttacaa gggaaaaacg ttctttccat gaaatatctt tgggtaacgc tttttcataa
     3181 atcccaattt gagccatttc cttaccccca gatacgaatg atctcatctt tgaagttttg
     3241 tgcggccttc atcggatcat ttgctgctgt aataccacga ccagtgataa aggtatacac
     3301 atcgatccct tcaaataagt gtaatgtgtg tggatttaat ccaccagtta cagaaacatt
     3361 aaagcctagt tcaatcagtt ttttgatctt cgataaatct ttttcacccc aagtttcacc
     3421 agataacagc gcatcacggc tttgatggta gattacctgc cggataccga tgttatgcca
     3481 atccatggct tgttcaaaag tccaattgcc gtagagttct acttgcaatt cacctatttc
     3541 tttttgtgct gcctccatag ttggaatcgt tgcacaacaa atgactgtca tccagtctgc
     3601 tcctgctttt gcaacattac tcgccactgt tcctccagcg tctgcacatt tcgtatcagc
     3661 gactagtttt ttatctggga acatactgcg aatacagcga atcgcttttt gtccttcctg
     3721 taagcaaaga attgtgccca cttcaataat atctactaca tcaccaactt tcataacatc
     3781 tcctaatgca tgttctaagt cattgtgatc taacgcaatt tgcaaattcg gcctgctcat
     3841 cttagttcct tcctttactt gtttttttgg taccactttg tttcagcaaa tttttcactg
     3901 atttctttga ctgacatcac atttttgata ccgataactg taactccttt tttctctgcc
     3961 tgagtaaaca tattcaaaaa attgactggc gtaaaaacta catcgtattg gccagctgaa
     4021 ctttttccct ctgaaatggc acaatgatgg attttggtaa ttggtacgtt tagttctttc
     4081 attgcttttt ccacacttct catcatcatc aagcttgttc ctgatccatt tgcacacgat
     4141 actaaaattc tcatcttaat ccctccatta tgagtgttgt ctaatcagat tttcttttat
     4201 actctctgct tttctgcata tgtgtcatag tcttctacaa tcaaaaaata accatcagga
     4261 tttttccgat attgtaactg cggaatagcc aataaaccaa tgacaactac ggcaattcca
     4321 gcataaccta ggactttcat gattactgta aacaatggcc aagctgttgc ccagtcgaac
     4381 atcccgatat aaccaccgta tgttgacatc ccaatccatg tagcgaataa tgctgaaccg
     4441 cctacttgta tgattcctga aagaaacggg aacaggcaag cggctttgaa tccacctcgg
     4501 ttattggcat aaactgctat aacggcatta tcaaagaaca acggaatgaa accggctatg
     4561 acgatcgttg gtgatttcaa taaaatcagt aaaccaatca tcaaaaactg tcccaaagca
     4621 ccaaacaaga acccaatcgt taccgcattt ggcgaaccaa agccaaaagt agcagctacg
     4681 tcaattcctg gaacagctcc tggtaaaatc gtgtttgaga ttccttggaa agattgagtt
     4741 agttcatcga cgaatgtccg aacacctaat tgcaagatcg ccagatatac cgcaaaattt
     4801 aaagaagtcg tcataatgta gaataagaaa ctctgccctt cttgcatgaa ttgcgcttga
     4861 atcaaataat ctttgcctag tacaatcaga atgatcccaa agaaaaatag catgagtaac
     4921 gaagtagcaa ccatattctc attgaaaatc gacaagaatc ctggtaattt gatatcttca
     4981 atttttttgt cttgcttgat tgatctattt ttcttttcat cccgcttttt catccactca
     5041 gctaattttg caaatataaa gattccgaac atttgctgat gtgccactgc aaatccggca
     5101 ccttctgtca attcctgcgt gattcctact gttaagttag aagctactgt ccaataacaa
     5161 cctaaaatca gtcccataaa aatcaatgct tgtatcctac ctagatcagg aaagcaaaac
     5221 agtaaaatcc aaaaagcggt agctgcttgt tgaacctgaa cgtttccggt agtgaaaacg
     5281 gaacgcagtt tcgtatattt ctgaaaacgg actaataaga tatttaaaac aaatgcaatc
     5341 agtaataaaa tcatcacatc tccaaaggta cggccaaatg tctccattaa tccagcatcc
     5401 acagcatttt gtccaaaata aggatctgta accatggcat ccaaattgaa ccgttccttt
     5461 agtccaacca agattggccg aaaattattg accaaaccac cagagccaac agtcaaaata
     5521 aagtaaccga ccgttgcctt taagaatcca gcgatgcatt catgaagcgg gcgtttcagc
     5581 aaaatatagc ctaataacac gataaaacca atcaggtatg ctggctgcgt caagatattt
     5641 gttgcaaaat acgtccaaat gcttaacaaa aattccccca tcttttttct cctctatcaa
     5701 ttaattatat tctgccatca caacttggta atcatcgatg gagttcgtag agatgagacg
     5761 ctcaaccaat ccatctgtca ttaataattc ggataatttt tggatatttt ctagatgttc
     5821 ttctggatct tttgctgcga gtgtaaaaaa caaactagcc gttttctcct cattctcccc
     5881 ttcactttca aaacggattg gctgtttcaa cttggtaaat gaaatagctg ttccaaaaac
     5941 accatcactt ttttctgaag aatgcggcat tgctacttga ggtgcgatca caatataagg
     6001 accgtatttt tctacacaat ggatgatctc ctccacatac gtttcatcaa taatttcctt
     6061 atcgatcaat acaagacagc tttcggcgat agcttcacgc caatccgtga tttctttatc
     6121 tgggtaatga atcaattgat ggtcaaaaaa gtatttcagc aagaaactcc ccttctttct
     6181 tataaaaacg acggaaacgg tgtatcattt tcaatcgaga agacatcttc aaatcctcga
     6241 taaaaattaa actcaagatc atctttgttt gtcggataga tgaatttccc gcctacttgc
     6301 cagatataag gtttaaatcc atacttcagg cgatcctttt tgaatttcca aatttcagtg
     6361 atctcttttg gatcagccat aaaattagac caaatatcgt agtggacagg aatcactact
     6421 tttgcattca atgactccgc catgcgcagc atatcaacag aagtaacttt gtctgttatc
     6481 cctcgtgggt tttcaccata ggcccctaaa cagacatcga ttttgtgttc attaccatgt
     6541 ttggcaaata aattggagta atgtgaatct cctgcatgat aaagattccc tcctgatgtt
     6601 tcgaataaat agtttactgc aatttgatcc atctcctgag gcatttttcc ttttaatacc
     6661 tcttctggat cttcacaagt gaccaacgct gtccgatcaa acgcttctaa agctactaca
     6721 ttgatgtctt tgattttgac tgcatcacct ggctttactg taatcgtttt ttcttcagga
     6781 attccccatt tttgccacgt gcgaaccact tcttttggcc caatgaatgt tgcttcagga
     6841 caattttgat gaactgctgc ggctgtatgg atatctagat gatcggaatg gatatgtgta
     6901 acgattaaag cgtctacatt ttttatcgca aaaggatcga ttacgaaagg ctgagtacgt
     6961 aaattaggct gcatattttg aacgccactc atacgcatca tctgatggcc ttttttcatc
     7021 atgccattcc catgactacg ttttcccgtc ccacaccata gatcacataa aatattcgtt
     7081 ccttcatgac ttttcagcca aattcccgta cagcccagcc accacatagc aaaagttcca
     7141 ggatccacta cttctgtttc gatttcttca ttcaagtatg tgccccactc aggaaatgtt
     7201 tctactaacc acttttcttt tgttacttca ttgacagttt tactcatttg cttgctcccc
     7261 ccatatcgtt gttccaactt acaagtaaat attaatgtgt atcagcatcc atgaaaaccc
     7321 ttttatatga ccgtttgttt tttatatgat tttctttcag taatcaatga ttgtttcagt
     7381 ataataaaga cagatatgaa tgtttaaatg aacaactagt caaataaatc catggatcaa
     7441 gatgtccctc aggaggcaaa agtcatgaaa aactcacttg tcaatattga aaaacgtcaa
     7501 aaagaaattt tggaactgct tcagcagact gaacaactat caactttcga attagctgaa
     7561 tcccttgatg tatctattag tacgatacga agagatttaa atttattaga agaaaaaaac
     7621 gagatcatac gcaattacgg ttattgttct tataattaca aaaaccagac tgattttgat
     7681 caatctggtc ctgaaaggat taaacaagcg attgccagag aagctagtaa atacattaac
     7741 gattatgata cgctgtttat caattctagc tcgactgcgt tgaaaacact ggattttctt
     7801 caatctaacc atttgacagt cattacaaac aacgtgaaaa tcaattactc tccgcacaaa
     7861 gataattgta gttatatttt aactggtgga gagatgcgct ttccaaaaga agcactagtg
     7921 ggcgacatcg cattaaatac gatttcttca gtcaatgcag acatttgtat cattggctgt
     7981 agcggagtcg atgtagtaca tggggtcaca acaaagatat taaatgaatc aacgatcaac
     8041 gaaatgatgg tcaacaagac aatcaaatgt aagattttag tcgctgatca tcgaaaaatc
     8101 ggactgacat caaagttcaa gattgctgat atttcagtct ttgattattt gatcactgat
     8161 aaattttgtt ctgaaaaagt attagaggag atcagaaata cggggataaa aaccattcaa
     8221 gtagattcat tttaagaacc aggacgtaaa tatgtaaata ggattatcaa aaaaatgaat
     8281 aacaaattta tcgataaatg aaaaacacgc tacgctcaaa ttgataaagg aatcccaaga
     8341 tgtatccatc caatcgatac catcataatc tattgcttct tttataaccg ttatatgttg
     8401 cctaaatagg aatcaaacaa tatcgaaaaa aggaggtatg acaaaagtca cacctccttt
     8461 tttcgaataa acggtattca aatcagaaca ctgatttcac aaccacttta tttcaggtcg
     8521 ttttcaatca cctttaccgc gtaatcattt ccgttgaaac gcgctatttt ttttgcgtcc
     8581 atatatcttt ctaaataatc atcttctcct aattgcttat ggcaagaagc aatttcataa
     8641 agcaagtatt ccaatgcata agtcgattta tgtttcttat tatactcaat acccttctca
     8701 cataattgga ttgctttatc ataatcctcg attttacggt aataggtggc gatatcataa
     8761 tatacaatgc agaccatcag attatcaaaa tcagtctcac tcaatatttt aagtgtacgc
     8821 tccacataaa ctttcgcaaa atctaattct ccttttgaca agtagatcat tgccacgttt
     8881 gcagctgctt gtaagtgatg gatatctgtt tcggtcgttt gttccaacac ttgattaaaa
     8941 taaaataatg ctgcatctgg ttttttcgca taaagatgat caagcagtcc ttgataaaat
     9001 aaaatttgat ttaaaagatc agaattactt actttttcct gatcgatcgt ttgtaaaatt
     9061 ttataagatt cttggtattt tgatacagaa ttcaatgatt gaatctcatt tatttttttc
     9121 ctgattttgt cttcttctgt ttctactaat atatcggaga tctcgacatt cagctttttg
     9181 caaagcgcac ttaagatatt gatgcctggc gacatattct tgttttcgat attgctgaca
     9241 gttgcttgtt cacaaatgcc ttctgctaat tgtacttgtg ttaacccctt tttctttcgg
     9301 tattctttta gtaatgctcc atctacttta atcaaaacaa tttccttccc tttaaaaaat
     9361 tatttctcct ttatatcata cacaaaaaaa agttctaaac aaccaaaaat gtttagaaca
     9421 gaataaaaat tttaatttta aaataatatt caaattaaac aactggcgta tctgctcctt
     9481 gatttctttt tgtgttatct gaataattat cgtttacagt tataacacat agtgaaaatt
     9541 gtcatggcga gaaagtctaa gaatattttt aaacataact atttctttct tacgtgtgta
     9601 taatgacgac agcacaccta tcaattaata taattatttt atttttttat gttctcatgt
     9661 ttatttttaa tgacacaact tctcgtattt atctggcaca acatataaat cgatcaaaaa
     9721 attgttgcgt gtttttttga aaattggagt ttcaacttga ttacaaaatt taaatttaga
     9781 agttgtgaaa acagcgagag acagtttaat attcaaaata tacataggct actcgaacgc
     9841 tgtgtttttt agtatactag taagaaacga agggatagac tcattccaat aaggaggatt
     9901 tcatgaagac aattcttttt gctcctgcag tattcaattt agctgagaca acacgaatga
     9961 ttgaggttgc caaagctatg aaagaccgtt attactgtgt attctttggc ttttctaaaa
    10021 cttttgctcc tttgatcaca gaaagtggct ttgagtatca tttactagcg ccgatcctta
    10081 cgaaaaaaca agaagaacaa atcataaaat ttgaccagtt gcgtacactt aaaaaccctt
    10141 ttaccaaaga aatggttgcc tctcgtatca ctagtgaaca gagtctgatt gctaagtttg
    10201 agcctcgact gattgtgaca ggttcgaatg tgacaatttt tttatcggcc cgcaataaaa
    10261 agattccttt agtatatgtt aaaccctatg ctttgagtga accttttttt caatcagagc
    10321 atgcgctgat gccccacgag ttacgtttct tcggcccttt aagcaatccc ctttggcact
    10381 ggacaaaaaa gcgtatagta aatacccgat ggatccccaa tgccttcaaa cacgctgcac
    10441 aaaaagaaat gcttccttta ccaaaaacaa gtttacaaat gatggatgct gatctaaatc
    10501 tcatcacaac gcctaaacta tttttggaag aagaacagct gccggaaaat tatcgaatcg
    10561 ttggtccaat ttttgctaag ctcagtactt ccttgcctga agaagtgcta acttttatac
    10621 agcgaaagca acaagaaaga aaacgtatta tctatttcgc aatgggtagt tcaggcaatc
    10681 cgcgtatgtt acgcaaaatt ttgttcttct tacaaaaaca accggatcta gcggttattg
    10741 cacctattga ctatctgctt gatgacattc aatctgactc agaagatctt ttaataacag
    10801 aatatctgtc tagtcatctt ttgcatgaat tcattgattt ttcttttatc cacggcgggg
    10861 aagggaccgt ccaaactgcc tgtgaatccg gcaaaccgtt tatcggtgtg gggatgcaat
    10921 ttgagcaaag ctgtaatatt gactattgtg taaaatacgg aaacgcaatc gccctcacaa
    10981 aaccagtgac tgaaagaaaa ctgcagattg ctctgcgcga agtttcttca cccaagattc
    11041 gtcagcaggc acgcacgctg caagccgttt ttccaataaa tggtccacaa caagcaattc
    11101 aagaaatcga aaccttcctg agcaaccacc caatatagtt tcaagtagaa aaagcttttt
    11161 taatagagac tcatcagtaa tttcgctatt cgagacccag acagtgaaaa tacaaaaaaa
    11221 gtaaatagtg gattgaaaag ttagagttac ttaaattacg gtgtctaaaa aacacaaaat
    11281 catccttaaa ataaaagatc tgacgctatc caaatgagca taggtagcgt ctgccattta
    11341 ttcttagtca aattctttga cacctttctc tggttggatc atctcaacgt ttcttcaaaa
    11401 gaaagaccat aataattagg caaatacatt ttcttaaaat gtgtcatata gatctagaag
    11461 catcgctttg tagagatttg tgccgctagt cccctcaaca attactaaat caaatgtagg
    11521 atttaccact ttatctttga taatcaacct ccctcttctg ttgagacaat gatctcgcaa
    11581 tccgatgcat actcctatat aaactgccag caatccccac atattgagta gatcatgtca
    11641 gaatgttcta tgtcgtcagc aacgatacac taaaagtcac gttcaccatg actgattgct
    11701 attcccagca caattatttc tatacacgtt tcatgatttg aataaacact cacatcatac
    11761 atattaccat tcatacacca tactgcagcc cctactatat ggttgtaagc tcccaaatct
    11821 atatttgact tgatcacttt ggtagtagat ctactaattc taaatccatt gctgataaaa
    11881 tttccatttt ttcctccttc tttctgccaa aacagttaaa acaataataa tctttatgta
    11941 aaaaagggct gtgacaaaag ccttgtcaca gtcccatatt ccgaataaac ggtggtcaaa
    12001 agcttgcatc tgcggtaata agttcaacca aacgaaaaat aagccaacat ttcacaaaaa
    12061 tttaagaagc aattttcgta aatttcttct tatttcttga agctaaccac ttctgttaca
    12121 accactttta gaacatttaa agcttcgatg tgacaaacca tctatgctgc tcgatcataa
    12181 aattgatagc aaaaattcca caaagtacat atgtccccca tgaaaacaaa acattcatat
    12241 gcggtacaat gatctttgga agaatcacga tcaaaaatga tgccagtgcc caaatccatc
    12301 gattgatttg aatatgatat gtagctagca tattcaatat aaggtgacta gccgttaata
    12361 aaaagataaa aacaaaaaat aaaatcagcc aatacattgt ttcccacctt ctaaaaacca
    12421 aacttagaca gtctggactt cgttttctcc acgactttgt tcatctgaca gcatcttttt
    12481 atcataagct tttacaaatg gataatagat aataaatgct atcaatgtgt tgatcatgat
    12541 caagataaca acacgccagt catttcctgc tgaaagataa ccgccgattg gagctggtaa
    12601 tgtccatgtt ggcaaaacag taaatctatt gaccatttct aatttgactg caaaataact
    12661 gataatagtc aaaactaaag gaactaagtt gaacgggatg aacatgtaag gattcaacat
    12721 cataggagct ccaaaaatta tgggctcatt gatattgaag ataccaggaa taatagaaaa
    12781 acgaccaacc tctttcaaga attttgattt acagaagaat ataaacaaaa cagataccac
    12841 gatcgttgct cctgcaccac caattgttac cgtccattga taaaactgtt caggagcgat
    12901 atagggtaat tgggtggcag ctgtaccatc tgccaatgcc tttgcattct catccaacat
    12961 gatcagccac attggacgaa caacagcccc tacaatactc atgccatgga taccaaatga
    13021 ccacaacaaa tggatcaaaa acattggtag taaaacacca agcaaattat tccctaaaat
    13081 attggttaaa ggctcgaata acgataataa ggcagcgttt acatcaaagc ccgcgacgct
    13141 tcgaatcagc caagtaattg ttaaaatgac tgcacctgga atcaatgcct caaaactacg
    13201 ggcaacagca ggtggcactt gttctggcat tttgatagta atatttcgat ttttaaagaa
    13261 acgatatact tccactgcaa aaatcataga taagatcccc gcaaacatcc cagatgctcc
    13321 caaagaactc ataggtaaga cccagccaag aggtgcctct tcaggagtta aaacgtttac
    13381 aggaacttgt aacataaaga acgttcctaa agataatata ccacctgtca cactatctaa
    13441 tccatatgat ttagctagat ccgaacccat aacaaaagtt gcataaatag ccatcaatcc
    13501 catcgtcatc ctaaagggta aaacaatcgt tgccgaataa ggggcaataa attcattcca
    13561 tccaggaaac ggtaaattga taaagaccat aaaaaatgtc ccaatcaaaa tcaatgacaa
    13621 tgttgaaaca accccattac gaattgctaa taaatgacgc tgtgtaccaa tttttgccat
    13681 tggtgggaca agtttctctt cgacccattt catcagcttt tccactaaaa ccactccttt
    13741 tcaataaaat atgaaccatt cattgcatga caacgcaaag aatgttcata actaattata
    13801 taattaagaa attttatttc aatatattct cttaaataat caaatttatt tttaagaatc
    13861 ctgaaaataa gttttctaaa tcataggagc aacaaaaaaa ttcgataatt aacaaaaatc
    13921 aaaatgatta aatctagtga ttgaaacata tcatttcaaa aaaattttgt aaattcaccc
    13981 ataaatatca ccctatttaa cttttttcat cgtacactaa aattcaaaac aactatcttt
    14041 aatctttact tcttggtttt gttttaataa atttccttca aaaatgcctg gagaaactac
    14101 aatgatcacc gtatcttttt gtgcttgctc caacttcttc aaatctataa tagctaacag
    14161 tgtcccttct tttacctcct ggttactttc aacccgtatc tgaaacggac ttccttcgag
    14221 ttcaactgta tctaaaccca tatggatcag tatttttctg ccagattcac tttcgatcgt
    14281 tatggcatgt cgggtaggaa atatattatc gattattcct ttaactggcg catggatata
    14341 tccatcatga ttcgttatag cgtatccaac tcctatcatt tttgaagaaa agacctcgtc
    14401 ttttactgct gttaatggaa taatttgacc actagctaca gcttttagaa tgtttcttgt
    14461 tttaaagaaa tgcacttttt atcacctcta ctttaatgct ggccaatatt ttttgtttac
    14521 tttgatcatt tcatctagta ttttttttgc gactctggca tttggtacgg tttgattcaa
    14581 tgtaaatgct tgtaaagctt tttgataaga accttcaaag taagcatcta ctagtaactt
    14641 ctctactgca acttgtgcct ccatcaatcc tttatgaaaa tcactgattg gttttcttag
    14701 tgtaattggc tccacaccag ttgcaccaac atatgccggt atttcaacaa ctgcatctga
    14761 ccgtagattc ggaatcgccc cttgattagg aacgattagc ataaagcgac gacgatcatc
    14821 attcaacaag gaaatagcta tatcgacgat atattgtcca tgctccccaa aattaaagtt
    14881 caatacttct tccacaatac cgcggcgtat tttatcagcc atttctttgg tatttttctc
    14941 tcgtccatcc atgaccatat tagcacgtgt atactctttg tcagtatgct caacgaccat
    15001 atcaggatat aagtaatact ctaagtaatt attcgggata ttacttggaa aatttttcgt
    15061 gatgactgac atcatttgaa aggttttttg ccaagtttta tctcctatat taaaatcggc
    15121 gacttgcatt tcttttgtta ttagtttttc aataatctct ggcataattt ctcgttgcaa
    15181 ttctttatca taaatcgatg tataccagcc aaagtggttt aaaccataat acgtaacgat
    15241 ccaattctta cgatcataac cgtaattcac tgcaatcgtt tcttcaattg aaatagtcat
    15301 atcacaggca ttaatcattt ttacattcgg atatttacga cgaacagctt cagaaacgat
    15361 cgactctgga tttgtataat tcaaaatcca tgctttgggg gcatagtctt gtatatgatc
    15421 gacaatttcc aataaacctt tgattgagcg catcccataa gcaaatcctc cgagtccaca
    15481 cgtttcttgt cctactaatc catgtttaag tggaatcttt tcgtcctttt ctcgcatctc
    15541 caatcctcca acacgaattt gtgagaaaac aaaatcgcat cctgtaaaag caagctttgg
    15601 atcatccgtt gcacgaatgc gaatatttga aaaacctttt cttttcagca taaatttaac
    15661 aatcaagtac atatccatat ttctttcttc atcaatatca tataaacgta tttcagataa
    15721 tggcaaccgt ttttcattgt tcaaaatggc ttgaacgatt cctggtgtat acgtactccc
    15781 gcctccagct atagtgataa tctgtttttt catcatcatc ttttcctttc tattcttcta
    15841 ataatttatt gaattctgcc ataacataag tcacttctaa gccgatgata atttgataat
    15901 ttttttcagt tggtttaatg atcccgctta tccctaattg cttgattcgc tgttcctcga
    15961 ccatggtggg atccactacc tctacccgta ggcgtgtatt acaactatag acatccagga
    16021 tattggcttt tcctccaaac gcttgtagca tttttgttgc catatattca taatttttac
    16081 ttgctaaccc taactctgtt tcttctaagg ttacttcatt gccaaactct gccttagctt
    16141 cacgtcctaa tgtctgaatg tttctcttat tgatcaaata ataaaaagta acgtaataca
    16201 aggcaaaaaa gaaaattccg attggaagaa tcaaaatagc attggtagcg attcgaaaat
    16261 tgatgagata atcaacaata catgctccga atgtaaaacc aattcgtata ttgaataagt
    16321 aacagacaat ccctgctaaa ccggcaaaaa gtgcatggat cacgtacaac ataggagaaa
    16381 gaaacatgaa actaaattca acaggctcag tgatatttgc aataaatgaa gttcctgcac
    16441 cgcttcccat gattcctttg accatctctt tattttcttt ataagctgct ttatagatag
    16501 ctagacatat agctggtata ccaaacatca ttactacaaa aaaaactgat aaaaacagtc
    16561 cagcctgagg atctccgttg ataaagcgtg tcatctctcc tctgaaaact tccttgctag
    16621 gagatgtata gcttcctaaa tcgtaataaa tatacgtatt caacacatgg tgcagtccaa
    16681 aggggatcag caaacgattc aaaaacataa atatccccac acctaatgct cccatatcaa
    16741 cgagcagttt tgcaaaatta tcaatcccaa tttggatgag cggccagata aagcccaaaa
    16801 aaacagctgc gattgtttgt agaatcatca ccatagtcaa tgggaatttt tcgcccgcaa
    16861 aataagaaaa aactactggt aatttttgtt ctttaaagcg attatacgtc catgccgcta
    16921 ctaaaccgga aataattcca ccaaaaatcc ccatattcac ttctgggtcc ataatttcta
    16981 agcctttttt caaggtagca atcgctaaaa aaccagtaag tgcaggtatt cctttgtcct
    17041 tatttttgac aaaaccgatt gcaactccta tagcaaataa gatatccaag tttccaaaaa
    17101 cggcatttcc agctgcacca attactggga tgttcagcat atcgtcagct gataaacgca
    17161 tgatcaatcc cgctaatggc atcgctgcaa ctggaagtag catcgctctt ccaagcttct
    17221 gcacatttga tttaacattc atttttattc ctcctctatt aaatatcctc atatgttact
    17281 aactcaatca aaggatcatt tttcactttt tgagcaaatg ctgaatgaat taaaaattcg
    17341 acttggtggg tacgaggata cacaaaactt gaaccattta acaattcctg atccaaatac
    17401 gctgcgtgac acatgacttc gatcgtttta tcttctctca atatcatgag taaacttgcc
    17461 aaatattctt ccacactcgt attgatttgt cgtttttttt catcagtttc tcctacgaca
    17521 tcgaaacaag gctcaaaata atcgacatga cgaacttcat cttttcgttc aaagttgctc
    17581 cgaaccggca aatcatattt ttttgctaat gtaacaacta cctcctgatt ttcatccaaa
    17641 gtatgagtat gatgatgact gtctaaatga gttggtatga tccccgcatg ataaattttt
    17701 tgaatctgag cattccattc ctgatacagt tgatctttgt caatgttaaa tggatgctga
    17761 taataagata aggatctgaa ctctccgctt tcgataagag tatcgacagt tttgaggagt
    17821 ggtttgccac acgtcaatgt taaatggact cctacaccta gcctaggcat ttcctttctt
    17881 agtttcacag catgctcgaa acccggcata tttgccatca atgtagttga tgtcaaaatt
    17941 cctcgctgat aagcatccat aatcccataa ttcacaccat ggctatagcc aaaatcatcc
    18001 gaattaaaaa tcacttttcc catttcattc tccttctttt ttctactaaa ataggcgcaa
    18061 ccaaataatc atagtccatt tactatgtgt catttggttg cgcctataga tagttacgcc
    18121 cttattttaa tagattcttg ctagatgaat tgccaaatag atgatttcat tttcttgaat
    18181 ggcaagttga aattgctgtt cgatgatatc tttaatcttt agtgctgtac aatacgcttt
    18241 gctatgttta aatgtttcag taaaaaaacc aatattttct actggcgttt cttcctgata
    18301 aatcaagcgt ttaaccagga atttgatatg aaccattaag cgatgataag tcagtgattc
    18361 tttatcataa acgtttgaat aggatttttc tagatgggac aaaatcgtgg tcgtcatttc
    18421 tactattttt aagctcttta aactattagg ctctcctagc tcagcattaa tgatatgcat
    18481 agtgatgaat cctacttcat ttgaccctaa ctcggtttga tatcgatgat tgattcgctc
    18541 gaccacttct actgctatat catattcatt tggataaagg aatctcattt ctggcaacat
    18601 aattgctgtg acatctctat cttctctttt tcgttgaata gcaaaataaa tatgatctgt
    18661 caatgtaata tagatatttg aatctaatgg agaggagatc ctagctgtta tcagttcaat
    18721 cacttcttcc gcaaaatcca taaattcgat tggaatctgc gagactatct cactgaatcg
    18781 tttgatcttt tctttagaat ctaatacaaa taatttgtca gcatcctcaa catcaaatgg
    18841 cttactcact ttagcagaaa agccaatccc tttccccatc gcaatttttt ctattccatc
    18901 gtcatcgatc aatacagcat tatgattcag ctttttcatc acatacaccg ttaattcacc
    18961 tcctctatat gataaaacaa aacaacttgc agactccttc atacatattt ttgtctccaa
    19021 acaattttta aagacaaaaa acgacctaag aaagaaccct ttcttaggtc gtgcctaatt
    19081 gaatagtagc accctacata atttacaaaa atagtttaac acataataaa agcgttatca
    19141 atatcttttt tactcgtttt atgataatac ttgtatcgaa ttgatttgga ggtaattaca
//
LOCUS       DAFEPQ010000048        18774 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.050, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000048
VERSION     DAFEPQ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.050"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(565..1131)
                     /locus_tag="LP543_001833"
     CDS             complement(565..1131)
                     /locus_tag="LP543_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL2321999.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1131..2087)
                     /locus_tag="LP543_001834"
     CDS             complement(1131..2087)
                     /locus_tag="LP543_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBL2322000.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2087..2743)
                     /locus_tag="LP543_001835"
     CDS             complement(2087..2743)
                     /locus_tag="LP543_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBL2322001.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3113..5131
                     /locus_tag="LP543_001836"
     CDS             3113..5131
                     /locus_tag="LP543_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL2322002.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5201..5407
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5558..7972
                     /locus_tag="LP543_001837"
     CDS             5558..7972
                     /locus_tag="LP543_001837"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBL2322003.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8320..9873
                     /locus_tag="LP543_001838"
     CDS             8320..9873
                     /locus_tag="LP543_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBL2322004.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSSH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10254..12038)
                     /locus_tag="LP543_001839"
     CDS             complement(10254..12038)
                     /locus_tag="LP543_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2322005.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12053..13774)
                     /locus_tag="LP543_001840"
     CDS             complement(12053..13774)
                     /locus_tag="LP543_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2322006.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13874..14635
                     /locus_tag="LP543_001841"
     CDS             13874..14635
                     /locus_tag="LP543_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL2322007.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15602..15870
                     /locus_tag="LP543_001842"
                     /pseudo
     CDS             15602..15870
                     /locus_tag="LP543_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16542..>18774
                     /locus_tag="LP543_001843"
     CDS             16542..>18774
                     /locus_tag="LP543_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322008.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6174 a         3329 c         3772 g         5499 t
ORIGIN      
        1 tttattttcg gaaaaagcta atatagctaa gattgaaaca tccatctgga atttatcttt
       61 tttactgctt tgatcataaa agtctgcata ttcatcactg atattttttc taataaattc
      121 ttccatcata aaaatcacct caaaacgatt atacgaactt acgttcttgt tgtaaagcga
      181 tatttgaggg gcaaaaaagg ggcataattt gactgatatt aagttagcta ttgatatatg
      241 aatgacatac aaaaatataa aatcttttaa aaatgcttat atatcaatgt ttttgccatt
      301 cttttatata tctgttatat gtcaaaaaaa tgtgttcaat ttcaatttgt atttgaataa
      361 agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaatca aaaacagctt
      421 ctcatgtttt tgatttggtt ggacttatta ccgcagatat aagcttttga gcaccgtgaa
      481 taaagaggtt atgaaatcaa cgctctaacc tgagtattag agaaaaaaac gcaaaacata
      541 tcatttatgt tttgcgtttt ttatttatct ttttttctca atacaatata aaataggcgg
      601 ctggttcttt tggttgataa accgataatt cagcacatta tatgtttcct gtggcagttt
      661 ggaacaaaaa gaatcaacca tatccagttc tttttctcca ccttcatgac catagtagac
      721 gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat atttcttcca tagctgtgcg
      781 tgtagtctct ggcatagtga tgattgcttt atcacttttt gggagatacc ctagattgaa
      841 aatccctgct ttgagatttt gttgttcagc tatcacttct cctaacgttt cgtggccttg
      901 tagaaacaat gagacacgtt cttctaactg acgctgttct agcctttcac gagtagcatc
      961 gattgcttgt tgctggatat caaaactata gacatgacca gtttttccga tgtgttctgc
     1021 tagaaaaacc gtatcgtggc cattgcccat cgttgcatct acgacgtgat cccctggttg
     1081 caagatttct tgcagtaatg tatggctgaa atgcaaagca gtttgcaaca tcagacgctc
     1141 acctttttcc gtacatcata ttttccttgg aagctatctc tgcgaagaag ttcttcatca
     1201 attgcattca agacttccca ttttttcaag ctccacattg gtccaatcaa ggaatcccac
     1261 ggcgcatccc ctgtcaaacg atggatgatg atctcttttg gtatcatttc cagctggtcg
     1321 caaatgacat tgacatagtc aggacggctc attaattgaa ggcgaccttc atgatagtcc
     1381 cgcaacattc tcgttttgcg catcagatgc atcaaatgga gcttgattcc ttggatatct
     1441 gaatctaaaa tggtgcgtct gacattttct ttcatcattt ctaaactttc gcctggtaat
     1501 ccgttgatca aatgcgtaca aacacggata ttatgtttgc gtaatttagc cacgccatct
     1561 aaataagttt gataatcatg tgctcggttg atcagttttg aagtttcttc aaaagttgtt
     1621 tgcaatccaa gttctaccca taaatacatc cgctcgttca gttcagcaag gtaatcaact
     1681 acttcatctg gcagacaatc cggtcgtgta ccgactgata atcctacgac acctggtaaa
     1741 ttgactactt gctcaaatcg ttctttgatt atttcaactg gcgcatgggt gtttgtaaaa
     1801 ttttggaaat aaacgatgta ttgttggaca tgaggccatt tttgatgcat catgtcgatt
     1861 tctttctgga attggattgg aagagggtct tcaggtgcaa cgatcatgtc tcccgaacca
     1921 gatacactgc aaaaagtaca accgccatgg gcaacagtcc catctcgatt cggacagtca
     1981 aatcctccat cgatcggcac cttgaaaatt ttcccgccaa actcatttcg taaagcgtaa
     2041 ttccaagaat gatatctttt atttccttct gaataaggaa aagtcattaa aagtccttct
     2101 ttctgtaaga gttatctatt tgttttcttt tttctctata cactggataa gtcaggcaca
     2161 gccaagaaag actgagtaag tagccgccga tgatatcagt aggaaaatga acgcccagat
     2221 aaatgcggct tatcccgaca cttactataa taaacatcat cagcaattga agcaatgtgc
     2281 gtaaggttct tttttgaaca aaagctggaa gcagaaatag taaagaaccg taaagaatca
     2341 tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt ttcaactact aagtgcgcca
     2401 atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg gttgatgatt ccagcaatta
     2461 caataaatcc agaaacgaac caaaatgttt ctgccctttt tttcttatac cacatccata
     2521 aagccgcaca agcaaataaa atgatgacgg taagaggatt accgaattta gtcataaaga
     2581 gttggtagct gttcagatca ggataagaag ctctgacaat gcctgtaaac cactgatcga
     2641 aaccagaaag ccacgacaga tggaacttta cgatccagcc caaaatgaaa aacaggacaa
     2701 gaaagaagct accagccacc tgaaatgatg ttttatattt catacaattc ctcctttgaa
     2761 gcaatgactt attatagcat ttttggtcca gcgaaaaaaa cggtaaagag ataaaaccaa
     2821 tgtatgattc ctctttgaat aaccaatatt cttataaaaa aaataactgt aatcggaaag
     2881 acattttttg gtaatgcaaa tgaaatgtta gggaaataaa atcagtgttt ttcgaaatcc
     2941 ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg aacagaagtt gttcattacg
     3001 gtaaattaca atatgattac aaaaaacaac aaagctctta aataactttt aataaatatt
     3061 aagagaagtt ataattatta ataatagata aattgaagtt ggaggataga agatggaaaa
     3121 cattgcaaga aaagaacgga gacgtttaga acagaccaaa cgttttcgaa aagcaaaacg
     3181 aggggcagca atcgtgggaa ctgcgatggt aggctgttct gtcgctgccc cattagtaca
     3241 gccggttcct gttcaagcgg atgaaacacc tgttcagttc ggcgcaagaa tcaatacagc
     3301 ggcatttatc acagaaattg ctacttatgc tcaaccaatc gctcaagcca acgatctata
     3361 tgcatcagtg atgatcgcac aagcagttgt agaaagcggt tggggcagta gtgcgttatc
     3421 tcaagcacct tattataatc tttttgggat aaaaggcagc tatcaaggac aaaccgtcta
     3481 catggatacg cttgaatttt taaataatca atgggtaaat aaaaaggagc ctttccgaca
     3541 atatccttct tttgctgaat ctttcaatga caatgcctat gtacttagaa atacttcatt
     3601 tggcaacggt tattactatg ccggtacttg gaagagcaac acgaaatcct atacagatgc
     3661 gacagcttgt ttgacagggc gttatgcgac tgatcctggt tacgcaggca aattaaataa
     3721 tatcatcgca acttacggat tgacccaata tgatacacca gctagcggaa acgcaggcgg
     3781 tggagcgact acaggtggca ataatactgg aagtgcaggt agtactggcg gttcaagcaa
     3841 ttctggtaat acaaactcag gttcagcaac ttcgaccact tatactgtcc aatctggtga
     3901 ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg gatcaattga tccaatggaa
     3961 caacatccaa aacaatttca tttatccagg acaaaaactg acaatcaaag gtagccagga
     4021 aaatggctct tcgacgaata attccggtaa caataccaac tcttcaggaa atgctggtac
     4081 atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt caatctggtg attctgtttg
     4141 gaagatctca aatgatcacg gaattacgat ggctcaattg attgaatgga acaatatcaa
     4201 gaacaatttc gtttatccag gacaacagct aatcgtatca aatggcggtt cttctaatgc
     4261 tggagcaaat actggctcaa atactgggaa tacaagtggc tctagttcgt ccagctcaag
     4321 ttcttctaat acagcttcag gaacgaaata tacagttaaa gccggagact ctgtttggag
     4381 cgtctcaaat aaatacggaa ttacgatggc tcagctaatc caatggaaca atatccaaaa
     4441 taactttatc tacccaggac aacaactgac tgtttcaaac agtggatcaa caaacagcac
     4501 gtcaggtaat acaggttcgt caaccaatac aaataacagc agttcagtta atactggcag
     4561 cagttcgtct caaggcactt ataccgtgaa agcaggtgaa tctgtttgga gcgtggcaaa
     4621 taaaaacggc atcacgatgg atcagttaat cgaatggaat aatatcaaaa acaacttcat
     4681 ttatccagga cagcaattga tcgttaaagg tggaacttcg gcaaatacaa atacaggttc
     4741 aactacttct gcagcgaaac caaatacacc gaatacgtct gctacgacaa gtacatcatc
     4801 cactggtaat acgatgtata ccgtgaaagc gggcgagtca gtttggagcg tagcgaacaa
     4861 acatcatatc acgatggatc agttaatcga atggaataat atcaaaaata atttcatcta
     4921 tccaggacag caagtgattg tgaagaaagg ttcatctcaa tcaacaaacc aacaatcagt
     4981 tactggttcg aaaacctata ctgtgaaatc tggagaatct gtttggagtg ttgctgacag
     5041 tcatggaatt acaatggctc aactaatcga atggaacaat attaagaaca actttattta
     5101 tccaggtcaa acgttgattg tagctaaata aaatagctgt tcgaagaaat aacgattgaa
     5161 ttccacaaaa atatttgtta ctataaggct acttaaatat gacaacactt gaaaaggaag
     5221 tagtaaaaag actgaagttt cagagagcgc atggctggtg agaatgtgca cggatgcttt
     5281 tgaactcgcc tttgagttgc ccttgctgaa tacccgagta agcaagcggc ggtgacggcc
     5341 gttacaacgt tcgaggcctt ttcactaagg taaaattagg tggtaccgcg tagattacgt
     5401 cctataaaaa gagcaatctt tttataggat ttttttattt cttttgtgaa gtttgagaaa
     5461 acaaagcaaa aggaaataaa aataaacaga ttctgccggt tttttcgtta tatttataat
     5521 agaaacaagt gttttcaata gaacaggagg acagcaaatg agttacaatc ataaagaaat
     5581 tgagaaaaaa tggcaaaaat actgggcaaa aacgaatact ttcaacaccc atgatgatcc
     5641 tgaaaagcca aaattttacg cactggatat gtttccctat ccatccggtc aagggctaca
     5701 tgtagggcat cctgaaggtt atacggcaac ggatatcctt tcacgtgtga aaagaagtca
     5761 aggatacaac gtgctgcatc caatggggtg ggatgcattc ggtctgcctg ctgaacaata
     5821 tgcgctagat acaggaaacg atccagctga gtttacccaa aaaaatatcg aaacattccg
     5881 acgccaaatc aattctttag gatttagtta tgactggaat cgcgaagtga atacaactga
     5941 tccggagtat tataaatgga cacaatggat ctttacgaag ctttatgaaa aaggtctagc
     6001 ttatgaagca gaagtagcgg taaactgggt acctgaatta ggaacagtta tttcaaatga
     6061 agaagtgatc gatgggaaaa gtgaacgtgg tggctatgat gtagtcagaa aaccaatgcg
     6121 ccaatggatg ctgaaaatca ctgcttatgc tgatcgctta ttggatgatc tagaattagt
     6181 tgattggcca gaaagcatta aagaaatgca aagaaattgg atcggtcgtt cagtaggagc
     6241 aaatattgaa tttaaagtgg ctggtacgga taaatcctat accgtattta cgacacgacc
     6301 agatactcta ttcggtgcaa cttactcggt tcttgcacct gaactagatc ttgttagaga
     6361 aattacgacc cctgaacaaa aagctgctgt agaagcttat atcgaagaga cagctaaaaa
     6421 atcagatcta aagagaacgg atcttgcaaa agaaaaaaca ggagtattta ctggtgctta
     6481 tgcaatcaat ccagttaatg gcaaggaaat cccaatctgg atcgccgatt atgtattatc
     6541 ttcatacggc acaggagcca tcatggctgt tccagcacat gatgaacgtg actttgaatt
     6601 cgctcaaact ttcggactag aaattcttcc agttatcgaa ggtggagatg ttgaaaaagc
     6661 tgcttacaca gaagatggta cgcacatcaa ctctgaattt ctagacggca tgaacaaaca
     6721 agaagcaatc gataaaatga atgaatggct agaagaaaat ggcgtaggga aaaaagaagt
     6781 cagctatcgc ttacgtgact ggctattctc ccgtcaacgt tactggggtg aaccaatccc
     6841 aatcatccat tgggaggacg gaacagtcac agctgtccca gaagaagagc taccattacg
     6901 gttgcctaag acaaaaaaca tcaagccaag cggtactgga gaatctccat tagcaaatat
     6961 cgaggagtgg gtcaatgtcg ttgatcctgt aacaggtaaa aaaggccgtc gcgaaacgaa
     7021 tacaatgcca caatgggcag gaagctcatg gtattacctg cgttatatcg accctcataa
     7081 caaaaaagaa ctagcagatt atgaaaagtt gaagcgttgg ctaccagttg acatctacat
     7141 tggtggagca gaacatgcgg tacttcattt actatatgca cgattctggc ataaattctt
     7201 atacgatatc ggtgttgttc ctacaaaaga accattccaa aaactataca atcaaggaat
     7261 gatcttaggc gaaaacaacg aaaaaatgtc aaaatctcgt ggtaatgtag tcaatcctga
     7321 tgatgtggtc gaaacttacg gtgcagatac actgcgcatg tacgaaatgt tcatggggcc
     7381 attagatgct tcgattgctt ggagcgaaaa tggcttagaa ggaagccgta aattcttgga
     7441 tcgtgtatgg cgtttgatcg ttgatgagaa caataaaatg cgtgaccgta tcactacatt
     7501 gaatgatggc aagcttgata aagtgtatca tcaaacagtg aaaaaagtga cagaagacta
     7561 tgaaaatctt catttcaata cagcaatctc ccaattgatg gtctttatca atgaagccta
     7621 taaagtggac gctctgcctt atgaatatat cgaaggattt gttcaactac tcgcaccgat
     7681 tgctcctcac atgggtgaag aactatgggc aatccttgga aatgatggtg gtatttctta
     7741 cgcaccatgg ccaacatacg atgaagcagc acttgttgaa gacgaagtag aagttgtttt
     7801 ccaagtaaac ggtaaagttc gtgcgaaaag caatgttccc cgagacttag gaaaagatga
     7861 gctagaaaaa gcagcattag ccaatgaaat agtgcaagaa tatattgaag gcaaaacagt
     7921 cagaaaagtt attgcggtac caaacaagtt agtcaatatc gttgctaatt aattggctct
     7981 ttgtcaataa ggactgatga gttgtgcaaa atcaaatctg ggccagaatg aacttcattc
     8041 tgtctcagat tttttgttat ttcaaattga ttaattgatt gatcaaaaag gttttgattt
     8101 tcatgttctc aaacacgttc aatgtctttt tcttctgaat ggtcttgtaa gagatctctg
     8161 taatcatcaa atttttgtct attaatctta gtattttttg atgtataaac tacctgaaat
     8221 tgagagtaca taccaaattc ataaattttt tattagtggg actagtgatt gtatggattt
     8281 tgacgcataa tgaatgaagt atgagaaaag gagagacaaa tggatcaaga actatttatt
     8341 atcgaagtat tatcagctaa acaaaaaatt atcgagaaag cgaaaaaaag agcgaaaaga
     8401 ataatgttca agcttttaag cttagtattt ttctctggtt tgcttttttt cggagctgta
     8461 tcgttcctat atcgccatat gagtgcagta gtccgttcag ctgaagggat gcaaaaatta
     8521 aataatttgt atcacttttc gtggggagtt ggcggaataa caattgttgt gcttattgtt
     8581 tttcttctac ttatttggct ccgttaccat caattgtcga gtcatttcga tcaatggctt
     8641 agtcaaagag cacaacagtt tttacataat aaaccaactt ctcaagatga tgactatctt
     8701 tattttgaga agacttcgac gagtcaagct tttcggatac aaaaaagtga ttgccagtta
     8761 ttgctgagat cacttgatga tttacctatc tacttaggcg aaagcaaatc aaaattcgga
     8821 atgaacacat tccatatttt tttactagaa aacgaaccca gatgggaaaa aagccagtct
     8881 tcgcctggtg gatacctgac aactaagatg agtacattag tcactggttt tttcctcatt
     8941 cttttagctg gaacaatcta ttatgggaga tacactcagc caaaaatgtc ttctcatata
     9001 aatgctgctt ctcacatgac aatgaactca actaacgata cgcatataca aaagacaaat
     9061 gaagggttgt tagctgatcc taagcaaaag gtaaatttga caacacaaaa gataaatgat
     9121 ctcaaacttg atacagagac gcactctctc tatatgacaa cagatagtgg tgctaattgg
     9181 gtatttgtgc cgattgattc gcaatggttg cgttttggcg aatacaccct gacaacgggg
     9241 ctgattcctg aaggttattg gatggacaac acatatgata tttccgccga tttctcatgg
     9301 tttatctatt cttctgggga tgatgtacgt ctgctgactt caagagataa tggtaaaaca
     9361 tggcaaacgt ctattatcac ccaatcagtt cagccgatac gttatcgaaa agcggtattt
     9421 tatggcaacg atggtctatt tatctgtaca tcagccagtg ggatgtctgc agaaactttg
     9481 tacatttaca ctactatgga tggcggaatc acttggataa aaagtggaag tacaatgatc
     9541 aatcagccag tacaaaatgt cagcttcgtt acaacttctt taggttttgt ttcgacacgt
     9601 gaaagtttgt tttatacaaa caacggagga cgctcattca aagaatcagt tctgtcgatt
     9661 ccggaaaact atcagacagg ggggatagat ctttttcaat caccaaatga agttactcaa
     9721 ataagcgcga atacgttaga gacaaaattt tatttgttga aaaaaggacc aatcgatgca
     9781 gggaaaatgt ttgcctgcct cttccgttca actgataacg gggaaacatg gcaattcgtt
     9841 cagcaactat cgcaagtaga acaaggagat taaagaagag agattgtgag cctgacgttc
     9901 agcaccgagt cacaagaaac aaattcaaaa aacagcttct catatttgtt gaatagtgta
     9961 actcgtgatt cggagttgac aagagcaaca agaagcgatt ttgaagtata gcttctcgta
    10021 ttttacaaga tcgtgtctta ttgccgatag gttgtctctt gaacaccgtt tattcgtaaa
    10081 aagggattgt gacaagtctt ttgtcactac ttaccgaacg aaactgaagg agctatccaa
    10141 aaagtatcat tagtattatt ctttgtgttt ctagtggcac cactagtaca tttaaaaaag
    10201 ctatcgatca tggactaatc atacatgatc gatagctttt tagataatgt ttgttacgct
    10261 atctgaaatt gactattata aagactggca taaagcgttg gtgattggag tagttcagaa
    10321 tgtgttcctt tttcgacgat atcgccattc ttcattacaa ggatcatgtc tgcattttca
    10381 atcgttgaca aacggtgtgc aatcacaaaa cttgtcctat cttttgtcac agcttccatt
    10441 gcttgctgaa tcaatgcttc tgttcgtgta tctacgctag aagttgcttc atctaaaata
    10501 acgacaggag gattcgctag aatgactcga gcaattgtca ataattgttg ctgtccttga
    10561 gataacgtgc cgttttcgct agaaatgatc gtatcatatc cgtctggcag tgttcgaata
    10621 aagtgatcac attgtgcgat ttcagccgct tcgataattt gttctcttgt ggcatccatt
    10681 ttcccgtagg cgatattatc agctaccgtt ccttcaaaca gccaggtatt ttgtaaaacc
    10741 atcccaaaag aacgacgtag ttctccacgt gaaagcttgg taatatcaag accgtcgaat
    10801 tcgatctttc ctttattcag ttcataaaaa cgcatcagca gattgactaa agtcgttttt
    10861 cctgcaccag ttggaccaac gatcgctacc gtttgttttg gctttactga aaaatcgact
    10921 tgattcatca atattttatc tgatgtgtaa ccaaactgta catttttgaa agctattttt
    10981 cctttcggtt gttctaaaag tcttgcatct ggaaggtctg gaatttcatc tgtttcatct
    11041 aatatctcaa atatcctatc gattgctgcc attgcggatt ggatcgagtt gatcacataa
    11101 gaagcggtag aaattggttc agaaatctga ttgatgtact gaagataagc ttgtaagaag
    11161 ccgatcgtca caccacctga tagaacaagc atagcaccga ataccgcact tatgatgaag
    11221 gcgagttgat taatgaaacg aatggccgga tagatagcaa agttcataaa ttgagcacgt
    11281 ttaaaagcgt tgtattgctt tttatttact tcgtgtatag cagcttctgc atgagcttgc
    11341 tgattgaatg ttttgaccac tagattacca gctaagattt cttccattcg attatttaaa
    11401 acacccagtt catactgatt ctgttcagaa atctgtttgt ttttgttcgc aatcttagtt
    11461 gtaacaattg aactgccaac gatcagcaat aagacgagca aagtaagctt gctttcgata
    11521 taaaacagca tcagaccagc agcagctatt gttacgacag aagtaaaaaa ttgattgata
    11581 cctgtcaata acacttgaga aagttgattg agaccggtag ttgtccgact aatgatgtca
    11641 cctacttgat gattgtcaaa aaacgacatc ggtaatgttt tgaatttttt tgtgatttct
    11701 tttcgcaccc tcaatgtgat cttttcgctt aacgctgcca ttgtccgttc ctgaataaat
    11761 gaagtgatgc tgcttaagac tgaacagccg atcagcaata agacagggaa caacaatgct
    11821 tgtttgatat cactgaaaga aagctgtcca atacctgttt ttcttatttc tgataagaga
    11881 tcatcgatcc cgattcccat gatcaagggc atggctacga ctaataggtt cccgaataaa
    11941 ctgcatagaa acaatccgta aaaatattta tgttcaggtt tgatcatttt ccaaaaacgt
    12001 atcaaagcac gcgtgttcaa cgtttttttc ttttccatcg tatcccttct ttctatattc
    12061 cttgtgattt tgcaaactcc tgataagttt ggcaattaga cagtagttct tcatgagtac
    12121 cttgtccaac gatttttcct tcatctaaaa caatgatgtt atctgcttgt tggattgtac
    12181 tgaggcgttg agcaacaatc acgagagttt tctccgacat gtgtttatga agggctgctc
    12241 ttaatgctgc atctgtttta tagtctaaag cggagaaact atcatcgaat acatagatct
    12301 ctgctggttt gatcaacgct cgagcaatac atatccgctg tttctggccc cctgaataat
    12361 tagaaccgcc ttgagcaaca aagctgtcca atccgtcagg aagggtgtcc actagttctt
    12421 ctaattgagc gatcttgatc gctttagaca actcttctgt tgaggcatat tcatttccca
    12481 tctgcaagtt gcttaaaatt gtcccgctga ataagaaagc cttctgaggg acataactga
    12541 tgtgggagcg gatctgttct tgagtcattt gagtaagagg aataccacca agggttactt
    12601 ttcctatcgt agggtcacta agtctcaaga gaagtttcgc caaactactt tttccagatc
    12661 cggtcccgcc aacgatcgct gttgtttttc ctttaggtat tgtgaaatca atatcttcta
    12721 gaactggatc agagatttca gtatatttaa atgtgacatg ttcaaaagca gctgcatttg
    12781 tctgatcaaa ggttggttca gctactgttt tgatcgtctc ttcctcaatt tcgatctctg
    12841 cttgcagtat ttcttctatc cgtcgtaatg aggcaaacga tttagggata atgaccaata
    12901 ccatagctgc gatcatcaag taagcaagtg tcagcataga gtattcgact acagcagtca
    12961 cgacaccgat ttgtaatgct ccttctgcta caagatttcc gccaaaccac aaaatagaag
    13021 cataaacgat tcccatgagc aaaaaagcaa ttggagtgac ccaagcaaat attcggttca
    13081 ctttgatcat ttgattagca tagtcagcaa agctcttatt cgttcgcttt tcttcatatg
    13141 attgattatc aaaggcacgg atcatattaa tcccagtaaa gaattctctc aatgtgatgg
    13201 tgatccgatc catttttggc tggatactca aagataaagg tgcccctcgt ttcatcagtc
    13261 gataaacaat ccatacataa aaaacaacag agatcaatgt aacccatgtt agtaatagag
    13321 aataacgaaa catcatgaga atggtgaaaa cgcaaataac cggtgctggt aagatcattt
    13381 ggcaaaataa aacaatcatt tgctggacat tatcgacatc attcgtcatt cttgtcaaaa
    13441 gagatccaga actaaaacga ctgatatcct tcattgacag ctgttggaat ttacggaaat
    13501 atttctcccg cgtttggaat ccaaacctag cggaagccaa tgctgaaaag taactagagg
    13561 caactgcagc gattgttcct agaaacgcta caagcagcat ttcaactcca atttttttta
    13621 taactgaggc ttcgccactt gctatacctg tgtcgatcag ctttgctact aattttggta
    13681 cacctaatgt accggcgatt tgtaaacaga tcatcacaaa ggtaaggaga gcgagtcctt
    13741 tgtttttctg taagaatgtt tttaagatag acatattatt cctccttttt tcctgatact
    13801 tttcgattct ataccttcaa gtaacttgaa ggtcaataga aaaaagaata aactgaaaaa
    13861 gaggtgagaa aaaatgaaaa aaatgtatac gattggccaa gtatcggaac tatttgattt
    13921 gccaaaatca acgatccgct attgggatga acaaggatta atccgttcaa gtagacaaga
    13981 agagaatgat tatcgattgt ttgatatcga tgatattttt atgctttatg atatcgtttt
    14041 ttatagaaaa ttagatatac cgatcaaaca gatgaagaat ttatatggaa aaacattgac
    14101 ggagttatat gagacacttg atgaaacaga aagaagaatc cataaagaac tggtggtgat
    14161 gaaacaaaaa caaaaagaga taagggagca aaaaaaacag ttgaaattga tgatcgatac
    14221 aaacgaggaa gaatttcctg tagaagagat cccttttgac tgtatgatct caacggagtt
    14281 tgaagatatt gtggaaatca aaaaatttct acctaactat tcaagctttg ggatgatgag
    14341 tatgccagct tccagttcac agacgatgta tggatttttt atcgacccta gtgaagtaca
    14401 tttatttact caagacgtga tttgggagaa aaaagatacg gctgtttatc gtcgattctt
    14461 attgaaatcc gagatgaacc atgcggaacg taataatatt ttagaaatca gagaaagaat
    14521 gtatgaaaaa ggatggaaaa caggagaagt gatcggccag tatctattga cgaatacgga
    14581 tgaaaacaat atcagaactg aatattatca tgcatggata gaaatgaaaa aataggaaat
    14641 atgtgtttaa gataactcga aaatgatggt tgttttagtt ataaagaaaa attttctggt
    14701 tcgattacgg gtttaatgtg ggggattatt agtcgtggat agatagaatt ttagtttttt
    14761 agaggaaata tgtctttaaa aatggaatgg ttgcaattct tagcgatata tacgatgtaa
    14821 ttgtcctctt aaaaaagtat ggaaaaataa ttgtattggg tgttatgtat aattcatggt
    14881 gaactgagac taatgagaag aaaaattcat attgttatga gtgagattgt atcagatgag
    14941 aatgataaat taaataggtg cataaaagca ttacacttaa aatacattgt tgcttacatt
    15001 attacaatat gttaataagg caaaaataaa tagatggatt gaataaacat attttttaat
    15061 caaatagtcc ctactgtgac tttcctcagc tatcggtgat actaccgata gcattttttt
    15121 gcttataaaa taattataag tatttagaga tttttcttct aacattcttc aaacaagaaa
    15181 aatcacaaag gttattgtta tttttatata ttctttttaa gaatgattgt ttttttatag
    15241 aaattttaaa aaataaaaca cgttattgat ggatagttag tttgttattt ggtaatatta
    15301 tagaagttga ctggtagtga tttatttttc gtcgttttat aaaaatgtta ttttttggaa
    15361 aaattcacaa atatgtaacg aatgtttttc tggaatttgc tgtgttaaaa agcagtatga
    15421 ctaatataaa tactattgtc atttatgcaa ttttttatgg atagtaattt tagaggggaa
    15481 tcaatgaagg aagaatagaa gggaaaaata gttgcaaata cgccagttag caaaaatagc
    15541 aatacatctt caagctttaa aattcgagag aatcatcttg gtagtggaat caactacaac
    15601 aaaaggatta aaccaattaa tagatagaat taccaatgga aaaatagaaa aggtagttat
    15661 tctatacaaa agttgtttaa ttcgcttcgg ttatgaactt agtgaaaccc tttgcaatat
    15721 atggtacaga atatagaaat tattgacatt acagaaaatg atgaagattt aatacagatt
    15781 gtcactattt gtagatacct tttacaagga aaccgagcca ataaagctaa gaagatgatt
    15841 aaggagttga tggaagacga tacaagttaa gaaagtgaaa ctacgcccaa ataaagagca
    15901 aaaagagtaa ttggacgtgg aaaatgcaag aaatgaacca tcgattcggt ggacagttta
    15961 ttagtaatga cggtttgaga acatccatga cgagaatgaa ggaacatcct aaatgcgttt
    16021 agttgaacca agtgccaaat aatatggcca aataagcgat cagttatcga tgaatcccaa
    16081 gtattctaaa ctatatattc attttgatgc taagcattga tatatttttt tgggattgat
    16141 gaaggttaac caatacaggg actcactgat gaaattctag agtctgacct tggtatcaac
    16201 gatttagtga ttgtttctaa tggaaaagta taaaagtata taagtacatt aacaaaataa
    16261 ctattagggt tacgctccag cttgtgcaag gcactggcag gaggaatcgt gaaatcataa
    16321 ttatggttaa gccacacgga caactaaaag tcatacaaag ctatcgaatc aatcaacaga
    16381 agacaatcgg caatcattag ctttgtttac agcttacatt gcaacgcttg agttgaaccg
    16441 aaaatacgag ggaggtaacg cagccatttt gagttgggga atctttttag aaaattatcg
    16501 ggtaccttgt tgtttagtca aaattataag ggggagtaat agtgaggaac aaatttcata
    16561 aactgttaag tatgtttcta gttatgctta ttgtgatcaa tcaatttggg aacataacgg
    16621 ttctagctga gtctttagca gaacagccag agcgttcgct gacgatcgaa gttgatcaag
    16681 aggaggctgc tttgggtgag cagttccagt tgcgagtcag aggaacacca gacctgctaa
    16741 atgcattggt cattgataag agtgaggatt tgattgaagg ggaagtgaag caactcaatg
    16801 attacgaacg tctgattact tattcttccc agcattcagg taaatacgaa ttgaccgcaa
    16861 gaagcggtga tattcaatca aatacggtct caatagaaat cagtgtacca gaagaaagca
    16921 caagtgagtc ttttaatgta ccagaagaaa gcacaagtga atcttttaat ataccagaag
    16981 aaagcttaag tgaatctttt aatgaagcca gatcatctga gccatctata acgcaggaat
    17041 ctcagattga aagacaagag gagctcttga attatgacat cggcatctct gctgagcgcc
    17101 ccactttgcc gagcaggcat acacaaacgt taaagggata cattcaagga ggcgctggcg
    17161 atattcttga agaatatggg gagatggtcg tacgagttcc tagagcatcg attggcttaa
    17221 ttgagttcat tagagtggag gtcgctgatg attcaccttt aaagccactg gcattttacc
    17281 aagaaggcac agacgttgtt tttccattta cctttgatta tgaaggatta aatgaagccg
    17341 ctcagtcgta tacattcagt atcacgtttg aagttgcttt ctttcaatca ggcggtactg
    17401 agccaaataa tgactttcaa gtgagtctta cttatgctga gcgagcagca aacacggaat
    17461 tttcaattac gcctattcct tcatcaagac ccattttttc gaaattatac tatggctcta
    17521 gagaaatcga cagccaagga aatcttggga atctgctgat tgacgggcaa gacgtgacca
    17581 acagtaataa atttattttg caggtaaatt atgaaggatc acgaaaagac ggtgtagtgg
    17641 ttaccgatgt actaccgcaa ggaacaactt tggcaccgaa tccgactaca tggagttcca
    17701 ctggaaatag agaacctatt tcaggtgggg ttcggatcgc agaagtaacg aatccaggtg
    17761 aagcaaacgt tagaactcat tatgtttccg aggaatttgt ggacaaagta tcttttaata
    17821 aggaaacgaa tacacttgtt gtggactttg gtaatctagc agagggtcaa tcgtatttta
    17881 ttgaatatgc tcttgaagtg accgatgtcg acttgttcaa tgaaggaaga caagaggatg
    17941 ggttttctcg gaatcgagcg agtatgacct acaataatca ggtaatgagc cgagactacc
    18001 gcttgagaat ccctgaaagt aatcttccgg caatctcttt aagaaaaagt gtggacaaaa
    18061 atgttatcaa tctcaatgaa aatgaactaa cgtatactct gcatgtctcg atgttttctg
    18121 gaacaagaaa tacgcttgtt attgaagatg ttttggaaga aggcatggaa tttgtgtcat
    18181 ttctatcaga tattccctct ttcattgatg ccgaagtcaa taatggaata gttcgtttca
    18241 gtgcgaataa tacgattggt ggtccgacaa gttttaccct ctcttttaag gtcaatgtag
    18301 aggcgtatgc ggtcggcgat caaattttaa actttgcaac gcttgtgaat agtgacaatc
    18361 gattgaatac gagtactgta agaacaagga aaatcgatgg gcgtattaaa gtaattaaaa
    18421 ctgatgagaa tggccaaagg ctggcaggag caaaatttga aattttatca agcgattcac
    18481 aagtgataca agaaggtata acggcagaaa acggagaatt tttaagtgaa ccgcttcctg
    18541 taggtagcta tcaagtccga gaaacagaag caccggaagg gtatctttta gatgcgacgg
    18601 tacatgatgt ttttataaat gaatactcca tagaagaagt gacgttaacc attgaaaaca
    18661 tccttgatac gggtggtgtt gaactaatta aacatgctga aggcagtggt gaagtccttc
    18721 aaggagctgt ttttgagtta cagaaccgag aaggagaaac gctacaaacc ggtt
//
LOCUS       DAFEPQ010000049        18566 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.051, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000049
VERSION     DAFEPQ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.051"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(653..1693)
                     /locus_tag="LP543_001844"
     CDS             complement(653..1693)
                     /locus_tag="LP543_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL2322009.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2042..2368)
                     /locus_tag="LP543_001845"
     CDS             complement(2042..2368)
                     /locus_tag="LP543_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322010.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2355..3158)
                     /locus_tag="LP543_001846"
     CDS             complement(2355..3158)
                     /locus_tag="LP543_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL2322011.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3511..3801)
                     /locus_tag="LP543_001847"
     CDS             complement(3511..3801)
                     /locus_tag="LP543_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBL2322012.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3937..4116)
                     /locus_tag="LP543_001848"
     CDS             complement(3937..4116)
                     /locus_tag="LP543_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322013.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4113..4463)
                     /locus_tag="LP543_001849"
     CDS             complement(4113..4463)
                     /locus_tag="LP543_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322014.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4456..5781)
                     /locus_tag="LP543_001850"
     CDS             complement(4456..5781)
                     /locus_tag="LP543_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBL2322015.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            complement(5950..6534)
                     /locus_tag="LP543_001851"
     CDS             complement(5950..6534)
                     /locus_tag="LP543_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2322016.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELMKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(6745..6948)
                     /locus_tag="LP543_001852"
     CDS             complement(6745..6948)
                     /locus_tag="LP543_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322017.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(6958..7380)
                     /locus_tag="LP543_001853"
     CDS             complement(6958..7380)
                     /locus_tag="LP543_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322018.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            7694..8308
                     /locus_tag="LP543_001854"
     CDS             7694..8308
                     /locus_tag="LP543_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2322019.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(8332..8682)
                     /locus_tag="LP543_001855"
     CDS             complement(8332..8682)
                     /locus_tag="LP543_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL2322020.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(8676..8939)
                     /locus_tag="LP543_001856"
     CDS             complement(8676..8939)
                     /locus_tag="LP543_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HBL2322021.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            complement(9177..10322)
                     /locus_tag="LP543_001857"
     CDS             complement(9177..10322)
                     /locus_tag="LP543_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL2322022.1"
                     /translation="MLQHRAYKFRIYPNQEQEILMTKTIGCSRFVYNYFLNLWNHEYT
                     TTGKGLSYNSCSAMLPQMKRNAETIWLKEVDSIAIQSSLKNLSDAFSRFFKKQNKRPQ
                     FKSKKHPIQSYTTKNVNNSIEIRNNFVKLPKLGFVKFAKSQEPKGRILNATIRKNSSG
                     KFFVSILCEEEIVEFPKSNSAIGIDLGITDFAILSDGQKIDNNKFTSKMAKKLKREQR
                     KLSKRALLAKKKGVNLFEAKNYQKQKRKVARLHEKVMNQRNDFLNKLSTEIIKNHDII
                     CIEDLNTKGMLRNHKLAKSISDVSWSSFVTKLQYKANWYGRKIIKVDKWFPSSQICSN
                     CGHKDGKKSLEIREWTCPVCHAHHDRDINASINILTEGLRLHVMGLA"
     gene            complement(10334..10735)
                     /gene="tnpA"
                     /locus_tag="LP543_001858"
     CDS             complement(10334..10735)
                     /gene="tnpA"
                     /locus_tag="LP543_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBL2322023.1"
                     /translation="MSEIHYGRGYVYSIQYHLVWCVKYRHDVLYGQVDIDVKQFLKQI
                     ADDNNIKIIEIESDKDHIHLLIECTPQHYIPSIVKVFKGASARLLFKKHPELKQLLWG
                     GHLWNPSYFVATVSENTEEQIRKYIQNQKKK"
     gene            complement(10902..11174)
                     /locus_tag="LP543_001859"
     CDS             complement(10902..11174)
                     /locus_tag="LP543_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322024.1"
                     /translation="MLHHEVIRRIQKDFSCMEEMKYFVQNVEEQLYLESETSQTKVTV
                     SHNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKSELVENFMD"
     gene            complement(11156..11572)
                     /locus_tag="LP543_001860"
     CDS             complement(11156..11572)
                     /locus_tag="LP543_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322025.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGACYIMK"
     gene            complement(11588..11839)
                     /locus_tag="LP543_001861"
     CDS             complement(11588..11839)
                     /locus_tag="LP543_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322026.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTTETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTELEN"
     gene            complement(11839..12045)
                     /locus_tag="LP543_001862"
     CDS             complement(11839..12045)
                     /locus_tag="LP543_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBL2322027.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            complement(12490..12750)
                     /locus_tag="LP543_001863"
     CDS             complement(12490..12750)
                     /locus_tag="LP543_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322028.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDQFTRLMGI"
     gene            complement(13116..13589)
                     /gene="ssb"
                     /locus_tag="LP543_001864"
     CDS             complement(13116..13589)
                     /gene="ssb"
                     /locus_tag="LP543_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL2322029.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSTTSPSPTTFEQRDTNNGLDASQNPFGGQSIDISDDDLPF"
     gene            complement(13592..14491)
                     /locus_tag="LP543_001865"
     CDS             complement(13592..14491)
                     /locus_tag="LP543_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBL2322030.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(14504..15097)
                     /locus_tag="LP543_001866"
     CDS             complement(14504..15097)
                     /locus_tag="LP543_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBL2322031.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(15173..15322)
                     /locus_tag="LP543_001867"
     CDS             complement(15173..15322)
                     /locus_tag="LP543_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322032.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(15338..17542)
                     /locus_tag="LP543_001868"
     CDS             complement(15338..17542)
                     /locus_tag="LP543_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBL2322033.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(17553..>18566)
                     /locus_tag="LP543_001869"
     CDS             complement(17553..>18566)
                     /locus_tag="LP543_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBL2322034.1"
                     /translation="ELKPKHERQNFLVRDDRLDHAVAFLWKDPQTNETVGASYQGTFI
                     DYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLNDTW
                     LVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDP
                     FTNQKVRCERGIANDWQVPKEYKVIYEEVAKEEKITPEAIMAIHKTENNLQLTNQLVS
                     AHKVNASFGQQLSVNDSIEAINLKDICREVAKELKACERVDGTYDFDRFYQEKGDINA
                     QILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQ
                     ERE"
BASE COUNT         5279 a         3543 c         2911 g         6833 t
ORIGIN      
        1 tactgaaatc aaggtattat tgggaatccc agcttaaatc atagataccg taagggattt
       61 tattctttat ttaaaacttt gcaacagaac cgtccgttgc gggtaatacc gtaacactga
      121 agggcacgca ttaaggcgtc atcttaatgc tttcctgtag gacactgacg gagattacca
      181 gtctcccgag ccttacaggg gcctttcttt tttttgtatt ttcaattatc acaatttatc
      241 gtccaaaagt gcagaacgaa aatcaattca tcacaagcat atcagacaat taaatttaaa
      301 acaactaaaa atatacaaaa aagtgtaatt gaatattagc agcactgcct gaaaagaagt
      361 aatatcaggc ttttcagctt ggggaaataa gtgaattacc tctcttcatg aattattata
      421 actcaaaaaa gcacatatgc aaacactagt tccctatttt tttgaatgaa aaataactaa
      481 aatatctaac gattatgact ttaaaaccta cgtttgtgat tgttttcata aacttttaat
      541 ctaaaaactc cggaataata gctatttatt tgtaacattt gtaataaaat taaaatcata
      601 aaaaagttct aatttaaaat tgatcatttt tatagtgatc gctttttatc aactactcta
      661 gccaattttc cgtactaact gttggtaatt cgttaaatgt ttctctttgt tcgtttgcaa
      721 gctttgccgc aatagctttt tcgacaaacc aattttttac ataaacgaaa attagattct
      781 gcaaattgtt tactttttca gttttttgtt tttgataagc tttcagcaaa gtcatatata
      841 atcctttgtc agcatcaatt ccataagatt ccagttcttc ataaacaaca actgtttgct
      901 ctaattcttc agcttttttc tttgcattat gaatagtttg gacggttttt tgagcgtctt
      961 cgtaagtact agaaaatgtt tttatcaagt ttaatacatt ttctggtaca aatgtattta
     1021 tactgtcctc tttcatgatg tcgacgaagt tatctagtaa aacttgattt tgtaactgat
     1081 cagtttcagt gtctattatg tgtctattag tgtctataga attagaatag aatagattgt
     1141 ctgcgatttt cgcaattacg gttgagtcct tgatatgact ggattttgcc gtatttgcga
     1201 ttttcgcaat tacggtggta tcattgattt ctcgcggttt tgccgtattt gcgattttcg
     1261 caattacggt ggtatcagta ttgtcttcct cttccatgat tttaaagacg tcttgattcg
     1321 ttacttctgg cttgcctaag tacaataaat taggcatgga ttcaatctta ccgtcacgtt
     1381 tatttactct tcctctcttc tgtttcagaa gacctgcttt ttctaattcc tttttgattt
     1441 tagatacttt accttcgcgg cattgtaaca atttcattaa ttcttcaact gtgaatatga
     1501 aaaatatatt atcatcagaa tctacccagt tatttttgac tgaataatca aatcgatctt
     1561 taagtagcat ataagcaatt tttgcatcat ttgatagatc catgtatttt tcgttagtaa
     1621 aaaatacctt aggcatttga taatattttt cacgataaat ttcttttgat ttgtaataat
     1681 taaaatcatt catagccaat cctctttctg taggatagcg ttttgttcaa aactatgtta
     1741 caatgaatta aacaaatata tctatccact atatattttt ctactgctga taagtcgcca
     1801 aactttgtta aagcagtagt ctttttttat ctttttttag tttaccttaa aaaataaatt
     1861 aaagcaaacc gctctatttc aaggtttaag ctcatagtaa ctatatcacg atattacaat
     1921 aatattatat cacgatatcg taatattaca atatgaagat aagcgactta tcagctttga
     1981 tactactgat ttttataaaa aaaatatcac gatattatga tatcgtgata ttgaatgtaa
     2041 attaaatttt attttgtttc tttgctagtt tatatccttc aatcaagtat tcttcaggta
     2101 tggactttaa tagcgcatct tctataacgt ctggtttact tttgtctgat tcagcaatag
     2161 tcgtccaata tttgagtgct gacattagaa ttggatttat acgaatgcta tctgctgttc
     2221 ttacactact tccttgatct tttatgactt tacttatatt tttcttggtt aattcgattt
     2281 cttctacttc ttttgattta aaattctttt tatttttatt tcctagtaat ctacttactg
     2341 cagcttgact gcgattatct tctgccatca aaatcaccaa cttcttttaa atattcttct
     2401 gtaaatgctt tatagtcatc ttcagctgtt gagggtttat taaaattgat tagatctgat
     2461 ccagcagctt gcgcttcgtc aacggttatt gtcctacgaa tataagtttt gaaaattttt
     2521 gcaccaatga ctctagaaat ttcggttgct gcagacgtca tttctttaga aatgttataa
     2581 cgcttattat acttagtaaa tagaactcct cgtacttcaa gcttgtcgtt cgtatattct
     2641 tttaccgttt caatagtttc acttaacttt attagtcctt ttaatgagaa atacgcagct
     2701 tcaactggaa tgataatttc atcagaaaca gtaaatgcgt tgactgtaag cattcctaaa
     2761 cttggtggac aatcaattat aatataatca tagttgtcct tgattggttg aagtactctt
     2821 ttaagcctgt gttcacttcc tacttgagta aattccctct ctgcagaaga aagggctatg
     2881 tcggctggca aaacatcaat tcctttatag tccagaatag catctgtaat gtcagcctcc
     2941 ccttttaata cttcataaat tgtaggttgg tttgttccat ctatatcgaa acctacatta
     3001 tctgtagcat ttccctgagg atcaatgtcc actaaaagta catttttacc gtaaacaaat
     3061 ttcaaacatg ctgctaaatt aacgctagtc gtagttttag aaactccacc tttttgattg
     3121 gctactgaca atattcttgc cattaaaata tcccccattc atcttatatt ataatataat
     3181 tatatcataa tattgtgata ttgcaatatc gtatatgcat tatattgtaa tattgtgtga
     3241 ttattttgtt ttaaagatat atatcggttc ggatataaag attattcata tgttattcga
     3301 aattaaaata atcacttttt attactttaa aattttttat taatgataag aaatatgttt
     3361 tgcgagcagc aactagaata atttaaacat tactaaactg tctatattgt tactaataat
     3421 tcccttctta tagtgcattt tcttagaaaa aaacaatatt acgatatcat gatattaaga
     3481 tatcgtaata ttatacaatt gtgattgata ttatatatgt ttttttattt ttttatcatg
     3541 tttaatcttg aaggaatcat attctttttc taaagacagg agcttttcat aaacatcaga
     3601 catgtttaaa ctagaggcca ggcttaaatc ataaacgaat gaagcaggta atgattttat
     3661 tctacgttgt cgggttatcc agcttgcaac cgttccttgt gggtaggcat ataactcaca
     3721 aaattgttct acagtgattc ctaaattctt gataatgtaa acattgatcg ggtgcgagta
     3781 aacaaatgta ttgatagaca tggtgcgtaa gcctactttc ttttattatt gcgtttgcaa
     3841 taatagttta acacacgcat tattctagta tctactccta tttgtaaaaa caaaaaagga
     3901 ttcatagaga taactctata gaatccgtaa aataccttat atatataacg ggaagatata
     3961 aaacctaaaa ttatgataac ctgctgcagc ttctacatag aaagagaagc gctcaacacc
     4021 aacctcattt atcaaacgtt ggccataacc ccaaatctct tctgatagct gatcaaaggt
     4081 tatatacttt agctcatatt gctggataat gttcattcta atcgactcca tttattatcc
     4141 tggtaaaatt cgatatgacg tatttcgtcg tagtgaattt tgtcttttcc aacgtagatc
     4201 cccagctcat cactacctaa gatcttgcca atgatatctt ctggatagta tccttcagcg
     4261 tttcgttctt ctttttgaat agatactgat ctatttttta tgactgcagc gttcaatact
     4321 tccgcaatct cttcagtcgt catttgttct tttgctaaat tttgactggt tcgttgttca
     4381 ttgtcggcat caatttcttc agtatgatca gataaataaa accctgccca ctttaaacgt
     4441 ccacgatcat tgtaatcaat catttttaat tcccccattt ccgcccgcat gaccaccaac
     4501 aagtgtgctt ctagtaatgc ttctagcgcc ttctaacatt gaagaagcat gtacaatcga
     4561 cgtaaaacca tatttttgcc gaatcttgtc aataatttta tctaaatttt cttcgtcaat
     4621 ttgcttttgt ggatcactaa ataaatccag ctgaagcgaa tcggtgtaca caagcttgct
     4681 ataggttatt ccaacatgcc ttacttcttg tccttcataa tacttatcaa acaagaaaag
     4741 acaataattc tgcaattcct ttgtattatc tgtgataggg atcttcatct ggtgtgaaaa
     4801 ccctggtctc gtttctaaga tcgacgtgcc aatgtgtaaa tggacacaac ctgttttaca
     4861 gttatgctgc ctaatgcgta tcgctacttg ttccgccatt tcttttacca ccagctcgat
     4921 ttcatttctt ctagtataat ctcgtggtaa cacttgtgaa tttccatatg atttttcctt
     4981 tgtcggttct ggtgggattg ctatatctgt tcgatcgatc ccattagcat ggaagtacag
     5041 ctgtaatcca atcacaccta atctattctt aatcgtataa ggattccagt tagcaaggtc
     5101 tctaatgctc aaaattccca tttggttgag cctttttttc attcgcgaac caattcccca
     5161 aaagtccgtc atttcgggaa tattccatac tttctcagga acgttttcgt acgtccattc
     5221 cgcaataaat ccttcattat gctttgcttc attgtctaag gctaatttag ccaaaagggg
     5281 attatcgccg acgcctaccg tagcaatcaa acctaactct tctttaatac gttcttggat
     5341 catttgagcg agcttcttcc ttcgctgact tcgtgtttct tcagtcgtaa aaagattcag
     5401 tgattttgtc acttttaaaa tcgattcatc gatcgagtaa atcagtagat cttcatcagc
     5461 cacatatctt ctgaaaatat tatttacctg catatttcgc ttgatatata ggttcatacg
     5521 tggtggaaca acgtgtagtg ttttaggaaa tggttgtggt aagtcacgtg gtctactcac
     5581 atttgtaata ccataccgct tttttgcttc aggagaagaa gctaatatca atcctgaacc
     5641 agtattatca gatcgactca taacaacaag ttctgttgtt aatggatcta aatttctttc
     5701 tatacattcg acacttgcgt aaaagctttt catatcgatt aaaaagtaat catttactgg
     5761 ctcttttgaa taatccatca taaataacac tccattcttc cacattatac aaacatatgt
     5821 tcgatgttgt aaagaagaaa tagctaaata tttgagaata agttatcagt agacacttac
     5881 aaaaaattct catactattg tcacttttct agataaatgg caaaaatttt cagcattaaa
     5941 tgacaaaaac taatgagaat ttgtctttcc atttttctcc tccatttttg ccaaataacg
     6001 ataaatggtg gttcttgaga cgttccaccg ttccgcaata gtcttaatag gtgtaccgct
     6061 atccactaaa gtcttcatta attccaaatc ttgttcagac aatttttcag gacgaccacc
     6121 aaaccgtcct cttgcacgag cagctgctcg tcctgctgcg gatcgttcca ggatcaaatt
     6181 acgctcaaat tctgcaaatg ccgcaaacaa atgaaacatg agctgtcctg tagaacttga
     6241 tttgtccatc gtaatatttt cttgaatact atgaaaactc acaccacgct cattcagtcg
     6301 attgacgatt gaaatcaaat cttccatatt tctacctaaa cgatctaagc gccaaacgac
     6361 aagtgtatcg ccagagcgaa caaaatcaat ggccatttct aatcctggtc gattactttt
     6421 tgccccactc atatgatcag taaaaatttt ttcacaacca aatttattta ggctgtcttc
     6481 ttgtaaatcc aaattttgta aaccagtaga tacccgggcg tatcctattt tcaaaaaaat
     6541 cactccttca ttaattttat tgtaacataa ctttatccat atagtttaac tgaacataga
     6601 tattgttatg ggtttatgaa catgaaatga attgtttttt tgaaaaataa agcaaaaaaa
     6661 gaaagtgtcc agaaatgggt gattttatga acactttcaa ttagaaaaaa agaatgtcga
     6721 ctagacactc tttttttaaa atcattattt ttctttttga tatgtttttt ggtctctttt
     6781 ttttcttttt tcttcagaat cttctagatt agaaattgat ttttgaattt tttgtgtctc
     6841 ctgtaaagtt cgctgaactt gtaaagctga ttcttctttc ggtaaattaa ttttacttag
     6901 aatatcgcta tcttgataaa aatttggtgt gttcacaaat tctggcataa aaaatcccta
     6961 ctttctagtt aaggttcctg aataagtcgc aatccaattg tttccagctg atttataact
     7021 ttgtaaatac aattttccat agtaccaata accatttctt ttttctttcc aattccgtga
     7081 ttgaggaggt ctagaaggcc aaccaacttt atcaaaaatc atcgttcctg tcactcgtat
     7141 cgttttagaa gctgctcgag caagtaatgg agaaactatt aaataatttt cagtaacagt
     7201 taagtcgtta gttgggttac caatggctat tccgttccta tctaaaggag ttaattcttg
     7261 gttattatct actacagtag ttggttcatt tgtactatca gcaaatgtag gaattgtagt
     7321 tccagttaca ctaattccaa tagttaaaat acctaaagat aaaataaact tatttttcat
     7381 tgtctatcac ctcgaaatca aaaataagaa aacgttttct taataaatct taacagataa
     7441 atcaagttat atactagaaa attcataaaa tatattttta atgtcataat cgtttaaatt
     7501 ttcttgattg acatttattt acaaaaaata ttgccattta aaaagaaaag tgacacatac
     7561 tatttcctcc atcattagtt tttgtctata taaggtatac cctaactaga cacgaccgcg
     7621 acattgaaaa atctggtttt ctcgtgtatg attagggtgt tttttaaata gttgcagaaa
     7681 gtaggtaaaa attttgagag ttgcatacat acgtgtctca tccatcgatc aaaatgaaca
     7741 acggcagatt gaagaaatga aaaaattcgg tgcagagaga attttcattg agaaacaatc
     7801 tggcgcaacg attactcatc gacctgtttt tcaagaagca ctggatttcg tccgagatca
     7861 agatattttt attgtggaag ctattgatcg acttggaaga aattacgacg agatcatcgc
     7921 ttcagtcaat tacttgaaga agaaaaatgt gcagctgatc atcacaagtc taccgatcat
     7981 ggccgaagca attggcaatc ctctcctgga taaattcatt aaagatttga tcattcaaat
     8041 tttggccatg atcgctgaac aagaacgtac cgaatctaaa cgacgtcagg cacaaggcat
     8101 taagattgca aaagcgaacg gtgtttataa aggacgtcct aagctataca gtgccaatgc
     8161 aaaagatccc caacgtcgtc ttgtttataa aaacatcgtt gaggatctaa aaaaaggtgt
     8221 tgcgatcgct aagattgcaa aagactacaa tgttacgaga caaaccgtat atagaataaa
     8281 aaaagacagc atggtcaatc acgaatagtt gatcatgctg ctgtttctat tttatttgct
     8341 tactttttga aaaactgttc taactttcat caataactcc tcaactaaac tatcatgcgc
     8401 tttttccaaa aaaatacact ctctggcttc ataatcaatt gtcgttagtt gatctaataa
     8461 tacttttcct gttgtcttca tttcatattc tgagtttata tttacatata gcggatagtt
     8521 tctagttgta ctagaaattg gagcaactgt aacaacgtta ctataatccg caataatatc
     8581 atgacttaaa acaatataag gtcttttacc tgtctgttcg tgtccagatc gaggtgctgt
     8641 attcaataat agaatatcgc cttgtctagg ttttctcacc atttttcatt acctactgct
     8701 tcacctaaat tttgaatcgt cacatgattt ctttcatcag tgtaattttc aaataactgt
     8761 tcaattgtta attcattttc tatattagcc gcttcgataa ttagccgctt ctgaccgttt
     8821 acttcttctt gtattatctt cactttgtct tctgtatgaa ttccaagata ttccataata
     8881 tactttggca atctaaggcc attactattt ccccattttt ttataggtaa catttccata
     8941 gtagacacct ctttctgtat atacatagta taacatacat aagaaaaaac gtatatactt
     9001 aattatcctt ttctattaaa ttagattaac attccatgaa tccttgaacc tctcatgact
     9061 aaagtcacga gattcttggg aatagagcat acttgcaagc gtatttcttt actcacagcg
     9121 gtttctctta tttaccaagc tatccccgta gttcctacgg tttttgatga ttggatctaa
     9181 gctaatccca tgacatgtaa tcttagacct tcggtcaaaa tattgatact agcattgata
     9241 tctcggtcat gatgagcatg acaaacagga caagtccatt ctcgaatttc tagagatttc
     9301 ttgccgtctt tgtgtccaca atttgaacag atttgactag atggaaacca tttatctact
     9361 ttgatgattt tccgaccgta ccagttagct ttgtattgta acttagtcac aaaactagac
     9421 caagacacat cagaaatgct ttttgctagt ttatgattac gcaacatacc ttttgtattt
     9481 aagtcttcta tacagataat atcgtggttt ttgataatct ctgtgctcaa cttgtttaaa
     9541 aagtcattgc gttgattcat tactttttca tgtagtcgtg ctacttttcg tttttgtttt
     9601 tgataatttt tagcttcaaa gagattgacg cctttctttt tagctagtaa tgcacgtttt
     9661 gacaacttac gttgttcacg tttgagtttt tttgccattt ttgacgtgaa tttattgtta
     9721 tcaatctttt gaccatcaga aaggattgca aaatcagtta taccaaggtc gattccaata
     9781 gcggaattag atttaggaaa ctcaactatt tcttcttcac acaggatgga aacaaaaaat
     9841 ttaccactcg aattttttcg aatcgtagca tttaagattc gccctttggg ctcttgactt
     9901 ttcgcaaatt taacaaatcc taacttaggt agttttacga aattgtttct aatttcgata
     9961 ctattattca catttttagt tgtataactt tgtataggat gttttttact tttaaattgt
    10021 ggtcgtttat tttgcttttt aaagaagcga gaaaaagcat cagaaagatt tttcaatgag
    10081 gattgaatcg caatactgtc aacttctttt aaccaaattg tctctgcatt tcttttcatt
    10141 tgtggaagca tggcagaaca agaattataa gataatcctt ttccagtagt cgtatattca
    10201 tgattccaca aattcaaaaa ataattatag acaaatcttg aacagccaat agttttagtc
    10261 attaatattt cttgttcttg attaggatag attctaaatt tataagccct atgttgtagc
    10321 atacggattc acctcatttc tttttctgat tttgtatata ttttctaatt tgttcttctg
    10381 tattttcgct aacagtagct acaaagtagc ttggattcca aagatgtcca ccccaaagga
    10441 gttgtttaag ttcaggatgt tttttaaaca gcagtctagc ggatgcgcct ttaaatactt
    10501 taacaatact aggaatatag tgttgtgggg tgcattcaat gagtaaatga atatggtctt
    10561 tatctgattc tatttctata atttttatat tattatcatc agcaatttgt ttcaaaaact
    10621 gtttgacatc aatatctacc tgaccataca aaacatcatg tcgatatttt acacaccaaa
    10681 caagatgata ttgaatagaa tagacataac cccgtccata atgaatttct gacatacaaa
    10741 aacacctcgc aattatttta atatatgtat ttctacaaaa tcaattattg caagacattt
    10801 cactattatt tagtttatat ttgtcagttg tttaacctag ctaaagctag gtcacaactg
    10861 aatccgattc atcccatgat taaaatcacg ggtgttctcg gctagtccat aaaattttcg
    10921 accaactcac ttttttctag gtaaacattc atgacgtatt tttctgtttc tagtcgtaga
    10981 agctccgctc ctttttttaa tgattcagga cgacgtttat acacatctaa acgattatgg
    11041 ctgactgtga cctttgtttg actcgtttcg ctttccaaat ataactgttc ttcaacattt
    11101 tgaacaaagt acttcatttc ttccatacaa ctaaagtctt tttgaatccg tctgattact
    11161 tcatgatgta acatgcgcca tacttattga tattttcaaa ataatctaac cattcacttg
    11221 aaacagtctt agcttttttc gtttcgataa atttcgcata cgttagttct aaagcttggt
    11281 attcagaagg tttgattgtg cttggtttga cgcctacttc caagcatagt aaggcttgga
    11341 aaaaccattt tgcattttgc tttttcagtt cttccagtgt tcgataatac gtcgcaatga
    11401 gattcatttc gacacctgat tcaatcattt cttccacagc taccgacaat ctttccttat
    11461 aatgtttttt tggaacacgt gtctcattgt ataggaatgt catatcctcc attttttgtg
    11521 ccattttatc caaaggtaat ttgacaaatt cgtcatagta attttgagcc atattttttt
    11581 ctccttttta gttctctaat tcggttaacc aatattcgtt ttcataagtt tccaagtttt
    11641 cttgataatg cagttcatat cctgcaatct tcactaggtc ttcaaatagc atagtcggtg
    11701 catcctctac taaaggggtt tctgtcgtcc agtaccgcgg aataagtaaa gcttctgcta
    11761 ctttttcaga aatgacttct ttgtccacca aaatttgaac gacttctttt acagtcaata
    11821 atttgtgttg tgttttcatc aacgtttctt tcctttctca tcaatcaaaa tataatcgcc
    11881 ataagacaag gtatattttt tcccgcatgt tggacattga tacaaacgat agcttttgat
    11941 acatttcgct ctttctccac aatcgcattc gacagtatgt ggtgcatgat agtttgggaa
    12001 taattcgctg aagggaacac cccaataatc aaatttcttt cgcatgattg attctgcctc
    12061 ctcttaggaa aggttttact tcctttcgtt agttctttct ttattgattt gttggccaga
    12121 atgaatcaac gaaaagcgac aaggacaagg gcgcgcagcg tgccatttac ccttgtcctt
    12181 gtcgcttttt ggtgataaaa ggcaacttgt tgccggtctg gcaaatcaat aaagagataa
    12241 cgaacaggaa cgaacctttt ggtgagagga agttttttct attttgtctt gctttgcaag
    12301 accctttatc ttagtgaacg tgcaacgttc acttcctttc ttctcgaacg gcggtggcgg
    12361 ttggagtgag agaggttttt caattgagaa ggcgctcgcg ccttcctaaa aaggcctttt
    12421 catttttctt gtttctatgt aaaacaaaaa gagctgcagt cctacattag gattgcaact
    12481 cttctttttt tatatgccca tcaatcgcgt aaactgatcg ggatttctaa catacatgct
    12541 agcaataact agctggtttc gtttattgaa gtgacctgtg acggcaatat tgtattttcc
    12601 atcatccata attagcaatt tatcgataat ttcaatatct gcaactacac aattaatcga
    12661 ttcatgaatc gtttgtaaag taaagcgaac caataacggt tgcgttttta ttactcggat
    12721 tttatcaagc aaaccaacat atttgttcat cacaatcacc tcgctaaaat tatagcaaaa
    12781 atccaatcat ttgaatattt caattatgct ataatagtct tgctgtctgc taccccggcc
    12841 taatctggca aggggaggag gttataggtt tgaaaattct ttttgatttt gtttttgcaa
    12901 tcatggcaga agtagccgcg cactatatct gcaaatggct tgattgtaaa tttgccaaga
    12961 aagacagtaa gcctacccgt taattcttat aatagaaacg taaaagaaaa ccactagccc
    13021 gccagctagt ggtttttggt tatagattga aaattctttt tgttgactaa attataacat
    13081 aaaccgtagc aaatgcaacc aatagaaaat gctacttaga atggtagatc atcatcgcta
    13141 atatcgattg attgtccacc aaatggattt tgtgaagcgt ccaatccatt gtttgtatcc
    13201 cgttgttcaa aggtagtggg gcttggactg gtggttgatt gatcagcgtg cgcacggctc
    13261 tctgtcgctg ctttactttc taatagttga aagttatctg ctacaacttc agtcacatac
    13321 acacgttgtc cttgctggtt gtcataagtt cgtgtttgaa tccgtccgac aacaccgatc
    13381 aagccgcctt ttttagctaa tgtcgccatt gtttctgctg gtttgcgcca aatcacgcag
    13441 ttaataaaat cggcttctcg attgccgttt tgattcgcga aatttcggtt gacggctagg
    13501 gtaaaagtcg ctactgctgt gccacttgca gtataacgta aatcgggatc tttggttaag
    13561 cgtcctacta atgtcacatt attaatcatc tttatctctc catttcttga ttcatttgtt
    13621 gtgatctttt aaactgccgt tttctttctt gattttcaat tttgatcaca cttttcatta
    13681 ggtgatcaat ttcgttgact gtatctgtaa atgaaatgga attcgcaaac gtgttaactg
    13741 agcgatattt ttcccactgc ttttccaaat agtcatttcc tctgatctta tccatttctc
    13801 tttcataata aaaggacaac ggttttgtat ccccacgttt tgtaaatgta tttcgaacac
    13861 ttttgtataa ctctttcgta tcgatatttt ctagtttcgg aatggtaaat aagtcgtagt
    13921 aatcttttgc tctcgaatta tctgtgccaa agctgaacgt agcacttagc ttttctgcaa
    13981 taatttgttc tgttggataa gctggaatct ccactttttt atcttcaaac attaattggt
    14041 gatagtgtgt atctacttca ggataaatag aatctcctgt tgtcacgtcc attttaaacg
    14101 gtacacgtac tttctctaaa ttagccacca atttaatttg aaatccagga taaaaatcgg
    14161 cttctcttgt ttccttcatt ccttgaatag agaaaacaat gccttctttt gtgggttttc
    14221 tagttaaatc attaaaaatg ctcttcaatt tttcttcagt taatttgcta tttctaaatg
    14281 tcgtatcgat atcaattgtt gtccttctat cgattccata ttttgatcct aataaaaagc
    14341 cgcctttcaa aatgaagtca tttttgtact ttgattcaga aatttgttca attagacgat
    14401 ccatcataaa atgccgttgc actaactgtg catttaatcc tgtactcttt gctaaatttc
    14461 ttgtttttgc tgcaaattgt gtcggtgtca tctttttcct ccatcaatat aagctttgca
    14521 tgtggtattt catttctttt tctacatgta gttttgtcat gtaatctctt aatttacttg
    14581 gatctctcaa tgggtcttcc atgtactctt ttaatgcttc taatttcatt tcatattcaa
    14641 attcatatct cggattccaa atatcacaaa gtgtacgctc tttatcatac atagtaatag
    14701 gatttccttg aaaggaagtt ccctctactt ttcctagttc ataaacttct ttcttcacga
    14761 aatgaaattg taactgctgc tcttttgtaa ttgaacgatc aacgttatag ccatggggaa
    14821 aagttaagtg aatgaatttc ggcatattcg tagaaagctg gtaaatatct aaagtcgttt
    14881 catgagagaa gattcccctg gaaaatcgtt gctgaagcca aaataaatca tctattccat
    14941 gaccatcctc tacataataa ccacgatcaa ctttgatcaa ttttccttga ttcaccaatc
    15001 tttgtacagt ggttgctgcg attccaaatt tcctggcttc cttcatagat aaatttccat
    15061 cgcgaatagc caacaattct ttcagtttat cttgcattta cttcccctcc cttcgttaca
    15121 aatacactac atcaaatata tcatagtagt ttatttgtaa caagattaac gtctatcgat
    15181 ctaattcgat ttctttttga atgcgctgct ggttctttaa ttgttgcagt cttcttttat
    15241 ttgcacgtaa tttctcttgt tctaaacgaa tttttgcttt ttgattttcg atttcttgtt
    15301 gtttgtgtgt tgtatttcgc ataggataac cccttcatta ttttgtagtg cggcgtgttc
    15361 ttttcttata gttcgttttt ttcttttcag aaaattcaaa gcctgttgta aagtcttctt
    15421 ttaaaacgat cgttgcttca aacgaacctt tctgaccttt tagtcctctg actgattggc
    15481 ttgttcggcc gttttgaatg atctctttca gttgtgtggc tgtgagtttc tttttggcaa
    15541 tcgttgtcca taatttaaaa tcacaggctt cagacgtaca tgccgccaca ttttgataaa
    15601 gcaagacttc ttgttcccga cattttggac aaacacctaa atgacgcgtt gcttcctgtt
    15661 tttcactggc tgctttcacc tcttgtaacg ttgatccaaa atctgttgac tggatttgtc
    15721 ccggtacgac ttccaacaag tgagagacaa atttttggat attagctaag aaagtagctt
    15781 gtgttccttt ctgctcacca atctttaata agtaactttc ccatttggcg gtcatttcgg
    15841 cacttgttag cagcggctct ttttccacgg cttggcaaag taaaatccct ttatttgtca
    15901 ccacaagctt atttttttct accttgatat attccttttg tttcaaagtt tcaataatat
    15961 ttgctcgtgt tgcttctgta ccgattcctt ctacttcttt caatatggat tgtgcttctt
    16021 cgctatccac ggtttttcca gctgttttca tcgctgtgat caacgtacct tcggtataag
    16081 gttgtggtgg cttcgtttct ttttcaagga cttgtagatc taccgtcacc gcttctcctt
    16141 ttgtcacttt aggaagtagc ggttcttctt ccttttcttt tctgtcgctc tctttgccaa
    16201 acagcttttt ccaacctaga tctactggta cttttccgac tgtctttagc tgaagctgct
    16261 gaacttgcgt caagatcgtt gtttcttcat aggtatactt ttccgcaaac atggccaccg
    16321 ttgtttttaa cacttgttca taaatagcct gctgcagtgg acttaattgt tcaaaagccg
    16381 ctgccgtcat gaccgtcttt gtaggaatga tcgcgtaatg ttcctggact ttcttattat
    16441 tcacatatcg actattcggt gtatgaatag gagtaggaat ggcttcggcc tttaaaaagt
    16501 gtttgtattc gtctaagtga tccaacagat acgcatactc cccttctgta atataagggg
    16561 tatctgttcg tgggtagctt agatatttcc cttcatacaa cccttgcatg gcttccaaag
    16621 tatctttggc actagctttc atgagctgat tcattttaga ttgaaggctg gatagagaaa
    16681 aaagacgtgg acttgccgca ctttttttct cggtttcaac ttggtggatc gtacctggct
    16741 gttttcccaa ttgaaccccc ttttctttta aataatcttc aaatgcttct tgcgtcgctt
    16801 gggtttcatt cggatcaagc ttcgcatcaa atgcgccatt ttgagcgatt acttgggctt
    16861 ttccttcaaa atatggttct ttttggaagt ttctaatctt ttcctgtaat tgataaatca
    16921 tgtaaagcgt tggggtttgg actctgccta aggaaaaact tcctggtatg cctttttgct
    16981 gcaagagtaa actatacagc gggctaccgt tcatgccgat caaccaatcg gcgatctggc
    17041 gtgcttgtgc ttcttcaaat tttgggtagg tctcttctgc atctcgtaac tgctggaatc
    17101 cctgataaat ggcttctttt tctaaggagt tgatccaaag tcttttaaat atctttccat
    17161 gatcggcgcc tgcttgttga atgatcgtcc aagctatcag ttccccttca cgatcgctat
    17221 ctgtcgcaat aataatggtg tcggccgatt gaagttgctg tttcaccacc ttgaattgct
    17281 ttttcttatc tttaggaaca tgatagtgat aatgtgtagg gaaaataggc aaatgctcta
    17341 atgaccattg tttccaattt tcatcgtaca tatcgggcga atctaactcg actaaatgac
    17401 caaaaccata cgtgatcaca gtttctcctg aaaacaatcg atcttggatc tcaaaatacc
    17461 catcttttcg ggtagcttta gaaaagctat cggcataagc cttcgcttgt gacggttttt
    17521 ctgctaaaat aacggttttc atcatgacga tttcactccc tttcttgctg tctaccttgt
    17581 tgaaacaaca tggcacgttt ttgctttctg atctcttgtt ggtagatttc atgcttcaat
    17641 tgatcattcc aatccttttt tacttcaggt acaaattcat gttcatgatt tttatagcct
    17701 ttgtagtatt gttctgcttt ataagagaaa agaatctgag cattaatatc ccctttttct
    17761 tgataaaaac gatcaaaatc gtacgtacca tccacgcgtt cacaagcttt taattctttt
    17821 gcgacttctc tacaaatatc tttcaaatta atagcttcta ttgaatcgtt aacagaaagc
    17881 tgctgaccaa aagacgcatt tactttgtga gcggaaacca attggttcgt taattgcaga
    17941 ttgttttctg tcttgtgtat ggccataata gcttctggtg tgatcttttc ttcttttgcg
    18001 acttcttcat aaatgacttt gtattctttt ggcacttgcc aatcattagc gattcctcgt
    18061 tcacaacgta ctttctgatt cgtaaatgga tcaactgcgc ccattaattg ttctttttcg
    18121 tagaaaatat gtcctgcacg gtcattatcc acacaaatct cgatggattg aggaaaggct
    18181 tgtttctttc tcaattcaga tactgcctca ccaaaataat gactgatcac atggtgtttt
    18241 aacccttcca tcgaaactaa ccacgtatcg tttaactgat cacgatttag tgcagcatag
    18301 ctaagtaaat caatactact ctcaaaaaat tttaactgct tagggtcacc gatctttagg
    18361 ttaaagccat gattcgctgt cgaattttta tctatatgct tatatgtgcc tctctcacca
    18421 aaacgttcat aatcaatgaa cgttccttga taactcgccc ctactgtctc atttgtctgt
    18481 ggatctttcc ataaaaaggc gaccgcatga tctaaacgat cgtctcgaac taagaaattt
    18541 tgccgttcat gttttggctt tagttc
//
LOCUS       DAFEPQ010000050        17965 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.052, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000050
VERSION     DAFEPQ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.052"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="LP543_001870"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="LP543_001870"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBL2322035.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="LP543_001871"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="LP543_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBL2322036.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="LP543_001872"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="LP543_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBL2322037.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="LP543_001873"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="LP543_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBL2322038.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="LP543_001874"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="LP543_001874"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBL2322039.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="LP543_001875"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="LP543_001875"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBL2322040.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="LP543_001876"
     CDS             complement(8053..8523)
                     /locus_tag="LP543_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBL2322041.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="LP543_001877"
     CDS             complement(8569..8994)
                     /locus_tag="LP543_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBL2322042.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="LP543_001878"
     CDS             complement(9061..9330)
                     /locus_tag="LP543_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBL2322043.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="LP543_001879"
     CDS             complement(9341..10936)
                     /locus_tag="LP543_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL2322044.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="LP543_001880"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="LP543_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBL2322045.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="LP543_001881"
     CDS             complement(14489..15049)
                     /locus_tag="LP543_001881"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBL2322046.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="LP543_001882"
     CDS             15402..16376
                     /locus_tag="LP543_001882"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBL2322047.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="LP543_001883"
     CDS             16546..17946
                     /locus_tag="LP543_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBL2322048.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
BASE COUNT         4884 a         3972 c         3238 g         5871 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaataa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaa
//
LOCUS       DAFEPQ010000051        17942 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.053, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000051
VERSION     DAFEPQ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.053"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..1220
                     /gene="terL"
                     /locus_tag="LP543_001884"
     CDS             <1..1220
                     /gene="terL"
                     /locus_tag="LP543_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HBL2322049.1"
                     /translation="VINMPPRHGKTFTARLFVLWMFGQNPKTKIITGSYNQILSSLFA
                     QQTRDGIMVENENVKAEYFKDIFPEVQIKYGDAAKGFWSLDGSEEKNYLATSPGGTST
                     GIGANYVIVDDIIKTAEEAYNERVLDSHWEWYNNTLAQRMERPRKQILIMTRWSSNDL
                     AGKMLTRRKNNVHQICYKAVQEDGSMLCNEIMTHDEYLDVVQEMNVDIAEANYQQEPI
                     DQKGRLYQKFLTYDTLPDNIIKIWNYTDTADKGADYFASPVFAETSDHDAYIIDVMYT
                     KEPMETTEIAHANMIIRNRVNKVRIEGNNGGRGFRRNSERITKERGYRGAVFEDFHQS
                     DNKLSRILANSAWVENHVYYPDDWATRWPDFYEAMVSYQREGKNAHDDAPDAVTGIAE
                     TIIEADDFEFIAY"
     gene            1232..2767
                     /locus_tag="LP543_001885"
     CDS             1232..2767
                     /locus_tag="LP543_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL2322050.1"
                     /translation="MFYSGITLKKYKRLRTKYSTQIEEGLFDPNAFMEDMKPFFDDRE
                     RKYLAYTSEKNEIDSREKPKTKIIKVNNKIHAGMYSVVVDQAVNHFTGIPIKWDYDVS
                     EQKRTFIQRMRDKLLGNDISAPSTPEEFETLTRQLSTMRFGMLDPDTATFQGATGLAF
                     RLLEPVETNDGWELRASNIEPWKAEKYENGAVYIKEKYDPYQKKFYEEMKVVTPKSIF
                     TYARYTDYPGTVQGAFKTLGNVPNPLGVINLTEFKNNTNRYCDFEVAEEISDAIDRVL
                     SDQQNEIEQFKLAYMLITGADLSEDKAKKMMEQLGIINLKDPNAKAGYVTKDLQKDFN
                     EYHFDLLKKQFYTVCKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAEVMAAYLDFAEKVTIDPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMIDVESRQSYPDGEYGGGIVESNRNEVLDQTSRTGRFSKTQNGKSNAK
                     ETK"
     gene            2847..3725
                     /locus_tag="LP543_001886"
     CDS             2847..3725
                     /locus_tag="LP543_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBL2322051.1"
                     /translation="MMEFYSTSDQKKYREYVEKNYKSLKMYDAKYKEFIEEYFPSFDY
                     AKVNRLLQMRSDIFRILAESAIDADVNKYFSDRLEDILQRSYVSNANVFAQILNVDLP
                     NYLSKEELERYLNYPWSGKTFSRRLWGNISSLEQKLSNAITTSVASGEGVLVALRTMR
                     EDPEICDMFKREESKYNKAIENLVRTEYAKFAQDGIEKSYIETGVDEYNVLTAKDEKV
                     CSICGGKAKNNPYKLSEAIIGENRAPFHGRCRCTDVPNMPKLGKDIDEEYERLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            3727..4044
                     /locus_tag="LP543_001887"
     CDS             3727..4044
                     /locus_tag="LP543_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322052.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYENMGCIVVRTDYKEGK
                     SSN"
     gene            4171..4794
                     /locus_tag="LP543_001888"
     CDS             4171..4794
                     /locus_tag="LP543_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HBL2322053.1"
                     /translation="MIMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            4809..5699
                     /locus_tag="LP543_001889"
     CDS             4809..5699
                     /locus_tag="LP543_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HBL2322054.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            5722..5937
                     /locus_tag="LP543_001890"
     CDS             5722..5937
                     /locus_tag="LP543_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322055.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            5949..6290
                     /locus_tag="LP543_001891"
     CDS             5949..6290
                     /locus_tag="LP543_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322056.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            6290..6661
                     /locus_tag="LP543_001892"
     CDS             6290..6661
                     /locus_tag="LP543_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322057.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            6654..7049
                     /locus_tag="LP543_001893"
     CDS             6654..7049
                     /locus_tag="LP543_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBL2322058.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            7051..7428
                     /locus_tag="LP543_001894"
     CDS             7051..7428
                     /locus_tag="LP543_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322059.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            7429..8037
                     /locus_tag="LP543_001895"
     CDS             7429..8037
                     /locus_tag="LP543_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL2322060.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            8037..8378
                     /locus_tag="LP543_001896"
     CDS             8037..8378
                     /locus_tag="LP543_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322061.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            8620..11409
                     /locus_tag="LP543_001897"
     CDS             8620..11409
                     /locus_tag="LP543_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBL2322062.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            11399..12139
                     /locus_tag="LP543_001898"
     CDS             11399..12139
                     /locus_tag="LP543_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL2322063.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            12136..14898
                     /locus_tag="LP543_001899"
     CDS             12136..14898
                     /locus_tag="LP543_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL2322064.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVNVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            14911..15819
                     /locus_tag="LP543_001900"
     CDS             14911..15819
                     /locus_tag="LP543_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBL2322065.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            15819..16442
                     /locus_tag="LP543_001901"
     CDS             15819..16442
                     /locus_tag="LP543_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322066.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAESYTNERIPNFTFEKLGRYRK"
     gene            16439..16888
                     /locus_tag="LP543_001902"
     CDS             16439..16888
                     /locus_tag="LP543_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322067.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLTNITFEKVGTV"
     gene            16888..17412
                     /locus_tag="LP543_001903"
     CDS             16888..17412
                     /locus_tag="LP543_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322068.1"
                     /translation="MADIVQLKEDGVAKYLKTHAKGIDGVEGVLVKATGNETVLGTKN
                     FKDGLQFNGLPVQAGMIERAITLADRSDTTNVTDVNGKIIRIGNIVFLTFNFKCGTWP
                     EGSETRWILKIPDGFKRDQGYPAQTALSLVRNASQPADARAFIDQSSIIQAKSGSGSS
                     YISGMWITQDPWPA"
     gene            17440..17847
                     /locus_tag="LP543_001904"
     CDS             17440..17847
                     /locus_tag="LP543_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322069.1"
                     /translation="MKTIYKVLYPMGYEEHQVEDNFPTSLPFVEVPPILFEKKEDETD
                     EGFGRRQQSQFFNFTENKWEEAVTQDYSKKLELLENLSIGLQVDNAALKKSNEELTEK
                     ADSMAQLNAKLMLNDLTINKKIEAIEKQIGGAS"
     gene            17847..>17942
                     /locus_tag="LP543_001905"
     CDS             17847..>17942
                     /locus_tag="LP543_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBL2322070.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRQFVPLCITDE"
BASE COUNT         6412 a         2523 c         3778 g         5229 t
ORIGIN      
        1 tggttattaa catgccgcct cgacatggga agacttttac agcgcggcta ttcgttttat
       61 ggatgtttgg acaaaatcca aagacgaaaa ttattaccgg atcttataac caaatcctgt
      121 catctttatt tgcgcaacaa acacgtgatg gaatcatggt tgaaaacgaa aatgttaagg
      181 ctgaatactt taaagatatt tttccagagg tgcaaatcaa atatggagat gctgcaaagg
      241 gtttctggtc tttagatggt tcagaagaaa agaattattt agcgacttct cctggtggaa
      301 catccactgg tattggcgcg aattatgtaa ttgttgatga catcattaaa accgcagaag
      361 aagcctataa cgagcgtgtt ttagattctc actgggaatg gtataacaac acgttggccc
      421 aacgaatgga acggccacgt aaacagatat tgattatgac acgttggtca agtaatgatt
      481 tagccggaaa aatgttgacg agacgaaaaa ataatgttca tcagatctgt tataaagcag
      541 tacaagaaga cggctcgatg ttgtgcaacg agattatgac ccacgatgag tacttagatg
      601 tggtacaaga aatgaatgta gatattgcag aagccaacta tcagcaagaa ccaattgacc
      661 aaaaaggcag gctatatcag aagtttctta cttatgatac gttgcctgat aacattatca
      721 aaatatggaa ctacaccgac accgccgaca agggtgccga ttactttgcg tctcctgttt
      781 ttgctgagac gtctgatcat gatgcttata ttatcgatgt catgtataca aaagagccga
      841 tggaaacaac tgaaatagcc cacgctaata tgattattcg caatcgagtt aataaggtac
      901 ggattgaagg aaacaatggc gggcgtggat tcagaagaaa ttcagaaaga attacaaaag
      961 agcgaggcta tcgaggtgca gtatttgaag actttcatca atcggataat aaactgtcta
     1021 gaatactggc gaatagtgct tgggtggaaa atcatgttta ctatccagat gattgggcga
     1081 cacgttggcc ggacttttat gaggctatgg ttagttatca acgtgaaggt aagaacgctc
     1141 atgatgatgc tccggatgca gttacaggaa ttgcagaaac gattatagaa gcagatgact
     1201 tcgagtttat tgcttattag gaggtgggtc tttgttttat tctggaatca cattgaaaaa
     1261 atacaaacgt ttaagaacga aatattcaac gcaaatcgaa gaaggattat tcgatcctaa
     1321 cgcttttatg gaagatatga aacccttttt cgatgatcga gaaagaaagt atcttgctta
     1381 tactagcgag aaaaacgaga tcgacagtcg tgagaaacca aaaacaaaaa tcattaaggt
     1441 caataacaag attcatgcgg gaatgtattc agtggtcgtt gatcaagcag ttaaccattt
     1501 cacaggaata ccaattaaat gggattatga tgtttcggaa cagaaaagga cattcattca
     1561 acgaatgaga gacaaattac tgggtaatga tatcagcgcc ccttctaccc cagaagaatt
     1621 tgaaacactc acgagacaat taagtactat gcggtttgga atgttagacc ccgatactgc
     1681 aacatttcaa ggagcgacag ggcttgcttt cagattactg gagcctgttg agacaaatga
     1741 tggttgggaa ctaagagcaa gcaacattga gccgtggaaa gctgaaaagt acgaaaacgg
     1801 cgcagtgtac attaaagaga aatacgatcc atatcaaaag aagttttatg aagagatgaa
     1861 ggttgtcacg ccgaaatcaa tatttactta tgctcgatat actgattacc ctggcacggt
     1921 acaaggagca tttaaaacgc tgggtaatgt tcctaatcct ttaggcgtta tcaatcttac
     1981 ggagtttaaa aacaatacaa accgttactg tgactttgaa gttgcagagg agattagtga
     2041 tgctattgat cgtgttttat ctgatcagca gaacgagatt gaacaattca agttagctta
     2101 tatgctgatc actggggcag atttgtccga ggataaagcc aagaagatga tggagcaact
     2161 agggatcatc aatctgaaag accctaatgc gaaagccggc tacgtgacaa aggatctaca
     2221 aaaggatttc aacgagtatc attttgatct tcttaaaaag caattctaca cggtgtgtaa
     2281 agcaattgat ttcaatgacg aagtctttaa gtcgaatagt tctggggaag cccgaaagtg
     2341 gcaaatcatt agtttagaag ctaagacaaa taccaaagaa cagtactttc gagaaggatt
     2401 gaaagagtgt gccgaggtta tggctgctta tttggatttt gccgagaaag ttacaattga
     2461 tccggaaaag atcattttca ccttctctcg ttcgcttcca acagatttaa gctacctagc
     2521 tgaggcattg cctaagttgg caccatacgt ttctaagagg acgattcaaa gtcagatccc
     2581 gttcgtcaca gatgttgatt atgaaaacga aatgattgac gtcgaaagca gacaatctta
     2641 cccagacggc gagtatggag gtggcatcgt tgagtcaaat agaaacgaag tactggacca
     2701 aacgtcgaga actggaagat ttagcaagac tcaaaatgga aaatcaaacg ctaaagaaac
     2761 taagtagtgt ctttcctgat gcgttgaaag aaattcaagc ggagttatta tcccaagctg
     2821 atttacacga tattacctat gctgagatga tggaatttta tagtacaagt gatcagaaaa
     2881 aatatcgtga atacgttgaa aagaactaca aatcactgaa gatgtatgat gcaaaataca
     2941 aagagttcat cgaagaatac tttccttctt tcgactacgc caaagtgaat cggctgcttc
     3001 aaatgaggtc agatattttt cgaattcttg cagagagtgc aatagatgca gatgtgaata
     3061 aatatttttc ggatcggtta gaagatatct tgcaacgttc gtatgtatca aacgcaaacg
     3121 tatttgccca gattttgaac gtagatttgc cgaattactt atcaaaggaa gaacttgaac
     3181 gttacctgaa ctatccttgg tcaggtaaga cattctcgcg tcgtttatgg ggcaatattt
     3241 catctctaga acaaaagcta tctaacgcca ttacaactag tgtagcaagt ggcgaaggag
     3301 ttctcgtggc attacgaaca atgcgagaag acccagaaat atgcgatatg ttcaaaaggg
     3361 aggaatcgaa gtacaacaag gcgatcgaaa atcttgtgag aacagaatac gctaaattcg
     3421 ctcaagatgg tattgagaaa tcttatattg aaactggcgt agacgaatac aatgtcttaa
     3481 ctgcaaaaga tgaaaaggta tgttcaatat gcggtggcaa agcaaagaac aacccataca
     3541 agttgagtga agcgatcatt ggtgagaacc gtgcgccttt tcatggccga tgcagatgta
     3601 cagatgttcc taatatgccg aaattaggaa aagatattga cgaagagtat gagcgtttat
     3661 ttggcgattt attagatgag tttgcacatg attcttttgg aattaatttg aaacggagga
     3721 agtagaatga aagatttttt tgaagcagta ctaacaatta atgtaaatgc tgatattgca
     3781 gaagcctaca aaacagctat tgagtctgag aaccatccta atggcttgag agaccattgg
     3841 aatggcaatt atgcctacgt ggttattggc gatcaaactg ttaattatca agataatact
     3901 ccagttgata agaataccgt taatttaacg attcaattat tatctcattc attaccaaat
     3961 ttaaaagaaa cagttgattg gtatgaaaat atgggatgta ttgttgttag aactgactac
     4021 aaagaaggaa agtctagtaa ttaggctttt ttattttgtc cgaaatgacg ttaaactagc
     4081 gcaatactgg gcttggttga atggtggggc gcaactatta aaactcaaag caatgcgggg
     4141 cgtgaaaacg aatcgtgggg cgaaaggaga atgattatga aacacaaatc attaatgcca
     4201 atgaatttgc aatactttgc cgaaggtgac gatcaaaagt tttcttttga tgacttcaaa
     4261 tcttttgtgg aatccaatga agaagctcaa aaatttattc agtcacaatc tcaaagtgtt
     4321 gccgacaaac aattggaagc ttggaaacaa aataacttag ataaaattaa acaggatacc
     4381 atcaaggaat atgaggaatc taagaaaaac aagtcacctg agcaaattca actggaaaaa
     4441 ctacaagctg aatttgaagc agaaaaagcg ttgcgtgtga caagtgataa taaagcattt
     4501 gttgcagaac aaattgctgg attagaacta gatggagagt taaaagagtc tatttctcaa
     4561 tttatgctaa ataatcttgt tagttcggat acagatttca ctaagaatgc tgttgaaggt
     4621 tttacaagtg ttttgaatgc aattaatgag aaacatgcag atgcattaaa agaactacaa
     4681 atgaagtctg catttggtgg aactcaacaa tcgaataacc aggttcagca gaacaatgaa
     4741 acattaacaa atccagaaga acaattagga caaattcttc aacaatttaa ctaggagagt
     4801 gaaaaattat gaaaaaaaca tctttaaata atttagagta tttggatatt tcaccagcga
     4861 ttaatgctat gcaagtacca aatacacctt ttttaagcta tttacttggt gctggaaaaa
     4921 cagagccagc aaactcgaca gaaattaaat ggcgtgaata tgatatcaac aacgatgatt
     4981 cttctgaaaa acttgagggc ggagaatatc cagatgctga atcaggtcga acttggttta
     5041 acaactatac tgaaattttt agaaaatcaa cctctgtatc tggtacatta gatgctatta
     5101 atgtgaatgg agtcggaaat gaattaacta atcaagtagc actacgtggt atggaaatga
     5161 aaattgattt gaaccgaaaa ttgattactg gtgtaaaagc tgatgaaaat ggttctaaag
     5221 gtcgtcgaat gaatgggatt ttgaacttga tcaattcagc aaataaggca gaaacagcca
     5281 ctgcgggtgc agtaacaaga aaagatatcg atactttatt taaattgatg tatgaaaaag
     5341 gttatatggg agaaaaacta tgcttgattt ctccagatat gcaggagtta atgactgatg
     5401 agttagatgg aaaatcaaca aaaattgttc agttcgggga aagagtaact tttggattgc
     5461 aaattggaaa tatcgtgtct aattacggta caggtattgc tctactagaa ccatcattgc
     5521 caaaaggaac aattgccgca atcgatacta attatgtgaa actacgtcca ttacgtgaat
     5581 ggagagcaga agaacttgca aaaacaacgg attctagacg tatcggtctt gtaggtgaat
     5641 actctcttga atacaatgct tcaaactctg gggcaatttt aaatttaaag tctgaataaa
     5701 agggagttag tactcccttt tttgatagga ggaattacag tggtaaaaaa agatgaaact
     5761 aaaaaagatg aagtcgtgaa atatagagta ggtaaaacta aaaattttgt tgggtttgtt
     5821 catcctaaaa ctcgtagatt tatcacagca gattcaaata acgaatttat catttctata
     5881 gatgataaag aagcaattgc aattttggaa gatgcaattg atgttaatga aatttaggaa
     5941 gtgatctgat ggatgaatcg ctaaaaacgg aaatcattga gtctacaaaa gaagattttc
     6001 cagatttgag tgaagaacgc ataactaatt tattagaaat aattttgcta gaaattgaat
     6061 catacaacac ttgtaaaaat gatatttcat gggaaaagtt aaaaagcgtg attagtgaag
     6121 tgttgtataa aataataaaa aatgaatcag aaaaaacagt atcttcagtt agacgtggcg
     6181 atacaacgat tagttatgct tcaacaacaa atgatgttag tgagttactt ctaggctatg
     6241 gtgatttgat acgaagagtt attggttgtg gaggattgga gtttttttaa tgaatgaagc
     6301 agatatttta gaaactactt atgaagatag ttgtattatt gaaagactga cggacattga
     6361 agattctaat acaaatatta ctattcaaga ttacaaaaaa gtatatgata atcctatttc
     6421 ttgcgctctt tcacaaggtc aaatcgatgg actagcagtc atagaagatg gagagatggt
     6481 aaatgtttca actgacacat ataaattatt tgttcatcct aagattaaac taaaaaaagg
     6541 agatcgaata acaataactc aaaaggccag tggcttaatt ttttctctat ttgctactaa
     6601 gcctttttac tatcctagtc attgtgaagt gaatttgata ggaagtgaga aaaatgggta
     6661 atctcaaata tgaatcaaat gcagaaaaga tcattgagaa ttttaaaaat atgactgtaa
     6721 ttgctcaaaa agaaggaata tccttcgtta acgattcaat gaataaagtc gttagtttag
     6781 ctaaaccatt aactcctgta aaatcaggta atttaaggcg aggatatcgt gtagtaaaag
     6841 ctagaaaact atcaagcggt cgaattgttg gagcagttat aaacaatgaa cattatttta
     6901 aatatgtaga agaaggtcat aggactaaaa atggtggctt tgtcaaaggg aaattcatgt
     6961 taactcgtgc aacgaatctt gcaaatatgt cttatattcc tcgaagattt aaacaaatgg
     7021 caataaaaat cgttaagaaa ggaaagtaac atgaaagatg aaatcattgc tgcaatcagt
     7081 agcaaattaa aagaaatcta tcctgatggg acaatatatc ttgattcagt tatgcagtca
     7141 actaaagatt tttatttcgt tttatcagta atggaatctg gaactgaaaa tgtaggaatt
     7201 gatattcaaa atgtttcttt cttaattgat attgcattga ttgataataa acctaataga
     7261 aatttaatta atgaattagt ctcacgttgt gggacttttt ttaatacgat tacaatcgat
     7321 gagcaaatat tatttccaaa agcctattta cctgatgaag cagatggtgt tcaacatatt
     7381 cgttttacat tagaatttcc acaatatatt gaatggagtg aaagataaat gggagaaaaa
     7441 agaagtaaaa ccggaattat ttctgtcgaa aaacctactt ggtttccttt ggaagatgag
     7501 acagggaatt tcccggtata tggtactgcg ttgccaatgg gaacagcagt aagtatcaaa
     7561 ccaacagcta attatgaaac aacacaagat tatggggatt cagtggttca agatcaattt
     7621 acagcttttg gtggtgcaga agttgaactg gaagcaaatg gatatactca taaagtttta
     7681 tctacaatta ctggagggaa aattgttaaa ggtggtgcct tgcgttctgg agaagatatt
     7741 gcgcaagatg gtgcgtttgc atatagacgt aaaaaatcga atgggaaata ccgttatacg
     7801 gtcttttata aaggacagtt tgctctagat tcagatgaaa cgtcaacgat tgaaggaagt
     7861 aaagtaagtt ttactcatcc aacttggaag ggttcatttg tagatgtacc aggacttgga
     7921 tatatgtatt cagttgatga agatgatgaa ggcgtggata aagcaatgat cggaaattgg
     7981 tttacaaaag tagcaattcc aattgaagaa gcagaacttt caggaggtac agaataatgt
     8041 caaaatatca aacaacgatt aaattaacga aaaaagatga agaaggtaaa tatgagcaag
     8101 tacaatttaa atctgctgaa tttttaccag gaacagttgt agaagatgct gcaggagtta
     8161 tggaagaaat gcaaacagcg actgataaac aatcagttaa aaaagcttta agtcgtgctt
     8221 attcatttat tgcagatact ttatttgagg gacaatttac tggtgaagat tattgcaaag
     8281 gaattgatgc tcgtgagatt gcctcattga caggaaaatt attgaagtct gttactgcag
     8341 gttttgatga aacttataca gagacgaaaa aaaagtaagt gaggcgctca agtcaccttc
     8401 atttaagtat tcgattactt accgagaatt agatataaag acgcaattac tcgaagcagg
     8461 ttggacgtta ccagaaattg aacgtactga cttggatgag ttaatgcgtc tttttgcttt
     8521 tagagatgcg gtaaaagagc atgaagatgt tgagtactat gataatttca ctcaatttta
     8581 ggaggtgata ctttgaataa cgatgaccta attctgaaga tgatactaga tgaatctggt
     8641 tttactgccg gtatgaataa tgctgtcaaa aagttgaact cttttgatga aacaatcgaa
     8701 agaaaaagta gaaatagtgg cagttcatta ggtagtattt ggaaaatatt tgctggaagt
     8761 tttctagcta gcggagtaac tagaatcgta ggagctggct ttgatctaat caagggttcc
     8821 ataagtggag cggttgatcg agtagatacg atgaataacg ctctacgaaa tttccaaaat
     8881 atgggattta gtaattcaga aattatgaag aatatcggaa agaatgggct tttatctcaa
     8941 ggtattcaag gacttcctac tgctttgaat gatgcgataa gtcatgttca acttcttgct
     9001 tcttctacag gtgatttaac tcgttcaact caaatattta aagctttaaa tgatggaatt
     9061 cttggtttcg gtggttcaac tgatcaagtt aatgaagctg ttattcaatt atctcaaagt
     9121 ttctcaaacg gaaaagtaga cgcacaaact tggaactcaa tgatcaacgc tcaacttgga
     9181 cctactcttt ctgctattgc taaaaagatg ggaattacaa tgggagatct gaaagaaggt
     9241 ttgtctcaag gtaagatttc tgttgaagaa ttccaaaatc aattaataga aatggatacc
     9301 aaaggtggcg gaggacttaa atcattaagt caaatcgcta aagattctac taagggaatt
     9361 aaaacctcta tacaaaatgc aaaaacagct gttacacgtg gtgttggtga ggtaatagaa
     9421 ggattaaata aagcattagt agattcagat ttaggtggat ttaagggaat tattgataaa
     9481 gtgggcagtt caatggaatc gttcttaaaa gtaattgctg caaatattcc tacagcagta
     9541 tcatttttaa gtaatttatt taacgaaatt caaaaatttg gttctgcttt gaaattcatg
     9601 atgccctttc ttgttcctgc agctaccgct tttggagcgt tcatgtttca acttaaaggt
     9661 ataccagcaa taataaaaag cttcaataat tttaagaatg ccataattgg cgttggaaat
     9721 tcattaaaga ttatgggggc aatagctgct gctaattcat ttgttttaat tattggtgca
     9781 gtcgttggtg caatagctgt tttcggttac tttatggcaa ccaatgaaga gtttagaaac
     9841 aaggttatat ctatttggaa tgatgtgaaa gattccgtaa ttggtgtatt aaaaaatata
     9901 aaggactggg gaattgatac ttggaattct gctaaagaaa tggcatcaaa tgcagttgag
     9961 ggtgtcaaag atgcttggtc aggaacaaag gaatggtttt ctaacacctg gaaaggtatt
    10021 aaggatgggg ccacaggttt atttgataaa acagtagaga cttcaagaaa tgcagtcgat
    10081 agtgtaaaaa atgcatggtc taatatgaag aaatggtttt ctgatacttg gcaaagtata
    10141 aaagattcag ccagagaaaa atggaatgaa attaaaggtt ctattatgtc ggttgctggt
    10201 ccattgatta caggtataaa gaatgcattt ttacatgtta ctttttatct agagacctta
    10261 tggaataatt tagttgagat tggtaaaaac gtctttgaga ttttaaaaaa tgttatactt
    10321 gcgccggttt tgtttattac ctccatgatt tctggtggat gggaagaaac aaaaaataat
    10381 atgattgctg tttggaataa tattaaagaa agtgccataa atatttggga atctataaag
    10441 aatatatttg taagttattt tacaaatatt tattttgctg cactcaatat ttggacaggg
    10501 ttcaagctta cattgataaa tatttggaat gaagtggtaa atcaagctaa atcgatttgg
    10561 ttaaacataa agtatttttt tattaatctt tggattgata taaaatattt tgcaattcag
    10621 aaatggattg aattgaagtt ttctattatt caaacttgga tcgatttaaa atataatgcc
    10681 attactactt ggaataatat taaacagttt ttcaaagata cttggaaaaa tattaaagat
    10741 acggcataca atacgtggat ttctataaaa aataccatga ttaatacatg gaataacatt
    10801 aaggactctt tctggaatat tgttactgga attgttaatt ctgctgaaaa tgcatggaca
    10861 aatctaaaaa atggtgtttc aaatgctatt aatcgggtaa aagaaatctt taattcgtta
    10921 agggaagtca atttatttga aattggcaag aacatcattg atggacttat aaatggtgta
    10981 aaagaaaagt ggaatgcttt gaaagatact atcaaaggta ttgcaggtaa tatcaaaaat
    11041 tggattaaag gagctttaga tattcattct ccatcaagat ggatgcgtga tatggttggt
    11101 aaaaatattg ttcaaggtat tatcgttggt attgataaag agcaaagtaa gttagatcaa
    11161 accatgacaa atttagtaaa aacaccgtct gttcaacctg tagtaaatgg aataagtcac
    11221 ctaccaatta cacaaacaaa gcaaaatata tctgctagtg aaacaaaaga aattcattta
    11281 catttaaatg tttatggaga tttacctgat tcaatggttc ggaagattac cacaaaaatt
    11341 aaaacagaat tgacaagaca aatgaaacga gatgctggtg cagtaggagg aacattatat
    11401 gcaacttaag agaggacaat tttttattaa tcaacattat tcttctgaat ttaatgtgta
    11461 tattcaaaac aggcctgctt ctgtttcagc tagtcgagta attgaattga gagagcgaga
    11521 aggaaatgat tctatcatta ttgataaagg ctattacaga aatgtgacta gaaagattga
    11581 gtgttactat aaagcacctt caattgatgt ggtgcaggag tgggaagatc gaatcactga
    11641 atggttagat atgtcgtctt atagtgattt tattctttac tatgatgaac aatatattta
    11701 tcaagctgta gtaatagaag gacctgaatt taaaggaaca agaaaaacag gaaatattgt
    11761 cccttttgaa tttacagtga gcattcgacc atttaaggaa aattacaatg gtagatttac
    11821 tattcgacaa acagaaactt ttgaaattta taacccagag aagtattctt caaaaccgct
    11881 tattaaattg agtggttctg gagatgcttc tttttatatt aataaggaca aatatgattt
    11941 gaaatcgttg gacagagaat tatttataga ttctaaatta gaagaggctt accgaaaatt
    12001 agatggtaat ttagaacatc aggatcaagt cactttattt ttagattttc catttttata
    12061 tcctggaaaa aatgaaatta aatggacgaa aaatattcat tcatttgaaa taatgcctag
    12121 gtggtggaga aaagtatgaa accaagaata tatagtccta ctgaaacaga tttctcaacg
    12181 aatggcttag gaattttaaa ggattgtaca agatgtgaaa tatatgaagt agctaatgga
    12241 aaatatgaat tagagttgga ttatccttta ggaactagat ttgatgaata ttttgaaaat
    12301 gactatcaaa taaaagcaaa gccaaacgat caagaagagt atcatatctt ttttattgat
    12361 gataaagata tagatacttt tttaaataca gtaactattt atgctcagag tcgtacaaat
    12421 cgacttggaa gacgggtagt cactcttgcg gaagtagact ctaaaactgg tcaagaagcg
    12481 atatcaatta ttgaaaccaa aatggataaa aaatctgaca tacgacttta ttctgatatt
    12541 acggccgttt ctagcacaat ctttgaagcg agaaatgttt taaattgtat tgctggtgaa
    12601 caaggatcat tgcttcaata ttggggtgga gaaattaaac gtgaaccatt taagctttct
    12661 ttgttaaagc gaagaggtcg caataatgtt ggaacgattc ggtatggaaa agatatgtct
    12721 ggtttaaagg tcaaattaga ttggacaggt ataaaaacaa gacttattcc atatgctgat
    12781 cctcaaagtg aggtaggtac gactaatcga atttatggtt cgccagtaga tagtgcctat
    12841 attaataact atcctgatgt gtatacagag catgttcagt ttacagaaga acaaggagta
    12901 aaagacatta agagcttaaa taaaatagct aaaaattact ttaaaacgat caatcctggc
    12961 tgtgataagc ctaaagtttc tattacggtt gaatttgata agttgactga tagtgaagaa
    13021 gcgaaagaat ttgcgaagat tagaaattat ggtctgtttg atacatttaa aatatatcat
    13081 aaaaaatata atatttatct tgaatcaaag gttagtggac tacaatacga ttctttatct
    13141 gaaaaaactt tgaaattaga agcaggagac attcaagttg ctttttatca gcaacaagct
    13201 gtaactattc aggataaatt aaaagattat gcaacgaata attatatgag tgattttaat
    13261 gattatgttt cttcaatgat tacaggtcaa ggcagtgcag gaggatatgt aagtttatgg
    13321 ccaaaagaaa aaccttccaa catctttatt atggataatc cagatttaga aaaggcaaga
    13381 gaagtactaa gaatgaataa aaatgggatt gctttttcta agaaaggatg gcgggggcct
    13441 tttacttcag cttggacatt agatagtata tttaatgcca attttataca aactggatta
    13501 atcaaagcag atatctttca aaattctttt aacaagactg gagatgtatt gaaattagtt
    13561 aatggactac tccaaatttg gaacaacaaa aagaaaataa tggagttaac taaaaaagga
    13621 atggaatttt ggaattctaa gggttcaatt ggaacgattg gaacaactga ttctgctggt
    13681 aatccttttc ctggggcttc tactccaacc cctattgaag ataattcttt agttattcgt
    13741 acaaatggag acggcaaata tattttgatt tctcctaaag taggaaaagg attagtttta
    13801 ttaggaaatg gtaaagcaat ttattttgga gacttagatg tacaaggaaa acttacagtt
    13861 aaaggaaaag aaataattcc tggacaaggt ggtggtccat cgggaggtgg agaatctact
    13921 ggtgggtatc ctagtgaatt aaaaacagat gcagaaaaga gagcttggag aatttacgat
    13981 attttgtgta ataacggatt tacgaaacaa tctgcatgtg gaatattggg gaacattcaa
    14041 caagaaacag gaggaacttt tgatcctgat actgttcaaa taggtggacc agcatacgga
    14101 ttagttcagt gggatggttc ttcatatcct cttgttggtc cagcaacatg ggatggaaaa
    14161 gttaatgttc aaaacttatt taatgctgca ggtattaaag aaccaataac gagcttagat
    14221 gcacaagttc gattgcttat ttggacattt acaaatggac aatggatggg cgtagtacaa
    14281 cctacgacgg ttgatggatt taaggcctgt actgatccaa gacaagcagc atatgctttt
    14341 gaacgaaact atgaacgtcc ggcagcgaca catcctgaac gtcaagatta tgcagttaac
    14401 tggtataaca aatttaaaga tttaaaacca ggaggagcta ctggagaagc aggactaaaa
    14461 catttagagt ctttgattgg acaacgaatt ggcaatggtc agtgttatgg cttgtctgca
    14521 gaatattcag gatatctggg tggttgtggc atgggtgctg gaacaaaata tggtttaact
    14581 catgtgatag gaaatacttc tgcagcatct gatattggta ttgcctatga ttggtctgct
    14641 gttggttgga aagtgattca aaaccctaga tatgatcaat tagtagttgg tgcaattatt
    14701 aattgggcaa gaggtggaca agtgggttca tggtttgcag acggaactta tggacatact
    14761 ggcgttatcc gaggcttagc taatggtcgt atgcaaactt atgaacaaaa cacagagtta
    14821 ggtatgattt gtggaaagtt agatcgccag tattatagtg caagtgcaat ttcttccatt
    14881 gtcataccac caaaataggg aggtgattta atggcaaagt ggaatgtcat attaagtaca
    14941 acagaaccct ataattacgt ggggatgatt caagttcgac aaggcaataa aaacactgaa
    15001 gttatggaag ctacaattgt cgaaaatggt cttccatatg atttatcaga atgtaaggta
    15061 tattttgaat cagttgttgg cggaaaatat ccagtccaat tagaaacaag aattgtggat
    15121 gctaaaaaag ggaaaatcaa atatattttt gataaatatt ccatgcagtg tttgcatcga
    15181 caaacagcca atttcattat atttaaagga gaagacttga ttggaacaac tcaagacttc
    15241 tcctattttg tcatcaatgc tgtttcaaaa acagaaggag aaatgggttc ttattggcaa
    15301 tcaattgaag atttaattgc tgatatgaca gattttatta atgaaaataa aggcgatttt
    15361 acggactgga tgaatgaaag gaaagaagaa tttgatcgct ggagagaaga acaggagaat
    15421 agtttccaag attggagaga aggacaagaa tcagattatt tgtcatggtt tgaatcaatc
    15481 aaggatattt tgaagtctat cgatccaggt ggagtaatgt tagccgaatt aatggatgca
    15541 cgtgttgata ttcaaggagt tcgtcatgcg tctatttcag agcgcttgct ggcagatatg
    15601 gattatctat atcagaaatt acgagcaaca cttttcacga ttgaatacgg tgagattgaa
    15661 gtgactgata ttttgcagga tgatctcttt tcagataatc acgaagtcga aaaagttgga
    15721 actgtagaat ttccaattga agaaggagct ttgattattg caactgttga tgatccaaaa
    15781 cagaatgttt tcactcttga gaaagttggg gtgatttgat ggttaaagta aagagaatga
    15841 tggagactga tgaatctggt gttaaacgtc aatttatgcc aattacacat gtttctgcag
    15901 ttttaggctt agaaaaaatg atatcaggac aatcaaaagt tctttcggtt aatggtaaat
    15961 atggtgcagt cattttaacg aaagctgatt taggattaga gaatgcaatt actgaactac
    16021 cttatgcaag cgagacaagc gatggtattc ttactgctga aatgtttcaa aagatcgtaa
    16081 atggagaagg tggaacatat attttgccga ttgctactcc tgaacaatta ggaggtataa
    16141 aagttgggga actcttagaa gttactgaag aaggtgtttt atctgcaact aagcaaacag
    16201 acttcaattt ctctgaagaa ttaaagggaa aattagaatc tctaaaaata ttaaaagcag
    16261 gtgcaaatat atcaattgca gaagatggaa caattagttc tacaggtggt agtgggactg
    16321 gtggtgttaa tcagtcatat gtagatcaaa aattccaaga agctgtaaat caagcagaaa
    16381 gttacacaaa cgaaagaatt ccaaatttta cttttgaaaa attggggagg tatagaaaat
    16441 gacagatatc gtaaaaataa aacaaagcgg tgtacaggtt tatccacaaa ctcattggaa
    16501 tgctatagaa ggcaaaccaa caacggtgaa gggagataaa ggcgatcctg gccaagcagc
    16561 aacaattacc ataggaaccg tttctagtgg ttcaaccgct tctgttacga atgtaggaac
    16621 atcatctgca gctagattta attttgtttt accaaaggga gataaaggtg atcctggaat
    16681 aaatgccaca actacagctg tagctacaac tacagcaaat gggttgatgt cgtccacaga
    16741 taaaactaag ttggatggaa ttgcagctgg agcacagaaa aatccaggta atgctacaac
    16801 tacgacagca ggtttaatgt cagcaaccga taaagtgaaa ctcgatggat taaccaatat
    16861 tacatttgag aaagtgggga cggtttaatg gctgatattg tgcaattaaa agaagacgga
    16921 gttgctaagt atttgaaaac tcatgccaaa ggaattgatg gtgtggaggg tgttttagta
    16981 aaagccacag gtaatgaaac ggtcttagga acgaaaaatt ttaaagacgg attgcaattc
    17041 aatggattgc ctgtgcaagc aggtatgatc gagcgagcta tcacactagc tgatcgaagt
    17101 gatacaacaa acgtaactga tgtgaatgga aaaataatca gaatcggaaa catcgtattt
    17161 cttactttta attttaaatg tggcacatgg cccgaaggat cagaaactcg ttggatttta
    17221 aaaatcccgg acggtttcaa acgtgatcaa ggctatccag cgcaaaccgc gctttcacta
    17281 gttagaaatg caagtcagcc agcggatgca cgtgcattca ttgatcaaag tagcatcata
    17341 caagccaaat caggttcagg tagttcatat atttcaggta tgtggatcac tcaagatcct
    17401 tggccggctt aacagattac taattggagg aagagtaaaa tgaaaacaat ttataaagta
    17461 ttgtatccaa tgggatatga agaacatcaa gtagaagata actttccaac atctttacca
    17521 tttgtcgaag ttccacctat tttgtttgaa aagaaggaag atgaaacgga tgaaggtttc
    17581 ggaagaagac aacagtcgca attctttaac ttcactgaaa ataagtggga agaagcagtt
    17641 acacaagatt attcaaaaaa attagaacta ctagaaaatt tatctatagg tttacaggta
    17701 gataatgctg cattgaaaaa atcaaatgaa gagttaacgg aaaaagcaga ttcgatggcg
    17761 cagttgaacg ccaaactgat gcttaatgat ttaacaatta ataaaaaaat tgaagcaata
    17821 gaaaaacaaa ttggaggtgc ttcataatgt ttagttttag cgatgtgaaa atgatgtatg
    17881 attggggctg ttttactgac gatcaagttc gacaattcgt tccactatgc attacagacg
    17941 aa
//
LOCUS       DAFEPQ010000052        17763 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.054, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000052
VERSION     DAFEPQ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.054"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            236..1042
                     /locus_tag="LP543_001906"
     CDS             236..1042
                     /locus_tag="LP543_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2322071.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            1054..2274
                     /locus_tag="LP543_001907"
     CDS             1054..2274
                     /locus_tag="LP543_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2322072.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2264..3850
                     /locus_tag="LP543_001908"
     CDS             2264..3850
                     /locus_tag="LP543_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL2322073.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            3889..6027
                     /locus_tag="LP543_001909"
     CDS             3889..6027
                     /locus_tag="LP543_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL2322074.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            6315..7706
                     /locus_tag="LP543_001910"
     CDS             6315..7706
                     /locus_tag="LP543_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBL2322075.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            7765..8574
                     /locus_tag="LP543_001911"
     CDS             7765..8574
                     /locus_tag="LP543_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL2322076.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            8546..9535
                     /locus_tag="LP543_001912"
     CDS             8546..9535
                     /locus_tag="LP543_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL2322077.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINPKTYFILYKKVLKK"
     gene            9649..10644
                     /locus_tag="LP543_001913"
     CDS             9649..10644
                     /locus_tag="LP543_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL2322078.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILIFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
     gene            10648..11790
                     /locus_tag="LP543_001914"
     CDS             10648..11790
                     /locus_tag="LP543_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBL2322079.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQLYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSKK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNFMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELIERDIK"
     gene            11787..13214
                     /locus_tag="LP543_001915"
     CDS             11787..13214
                     /locus_tag="LP543_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBL2322080.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIVGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIIIFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEIRKITRK"
     gene            complement(13281..14570)
                     /locus_tag="LP543_001916"
                     /pseudo
     CDS             complement(13281..14570)
                     /locus_tag="LP543_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(14750..15160)
                     /gene="tagD"
                     /locus_tag="LP543_001917"
     CDS             complement(14750..15160)
                     /gene="tagD"
                     /locus_tag="LP543_001917"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HBL2322081.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            15286..16677
                     /locus_tag="LP543_001918"
     CDS             15286..16677
                     /locus_tag="LP543_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBL2322082.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            16810..17757
                     /locus_tag="LP543_001919"
     CDS             16810..17757
                     /locus_tag="LP543_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBL2322083.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
BASE COUNT         6090 a         2583 c         3316 g         5774 t
ORIGIN      
        1 aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt tccaccaaca caaaatatta
       61 tagaacccct tttttggttt attgcaattg tctagcatct tctgtaaaat agaaacgtta
      121 gagaaaatct tgtatcatag aaaattaaga aagaaataag aacgcagctc ggtaccaaaa
      181 atatggtaaa atggcacttg actgtaattc actgaaatcg cttggaggaa cacaaatgtt
      241 taaagatttg tatttattta tgaaagacat ttatcagaat agaaaattac tgctacagtt
      301 ttcgatcaat gacttcaaat caagatatgc aggatcgttt ttaggaatct tatgggcgtt
      361 tatcaatccg ctatttactg tattgattta ttggctggta tttggtgttg gactgaaagc
      421 acaaatgatc gatgcaaaat acccatttat cgtatattta gttaccggga tgattccctg
      481 gttcttcttc tcagatgcat ttgcatcaac tacggttgtt tttcgggaat atacttattt
      541 agtaaagaaa gtcgtattca atattcgtat cttgcccacg acgaaaatcc tatccaatct
      601 gtacacccat ttattcttca ttctgattgg catagctgtc gcgcttataa atggtatata
      661 tccttcagca atgtctttac agctgattta ttatttattt tgcatgtgtg ctttcttgac
      721 aggattgact tggctgacgg catcgatcca accattttta cctgatatca tgcaattaat
      781 tacgatcgtg ctgcagttga tcatgtggac attaccgata ttatggagcc ctaatcagtt
      841 tgattcaggt cttgtaagca tattgaaatt aaatccgctg tactatgttg ttcaaggtta
      901 tcgtgagtca tttttaagtg aagcttggtt ctgggagcat ggaacatata ctttgtattt
      961 ctgggttttc acgttagtaa tgttcgtaat cggctctgca gtgttccgta gattgaaacc
     1021 acatttttca gatgtgctgt aaggagaata gctatgacag aatatgcaat tagactaaaa
     1081 gatatcacaa aatcttataa tatgtacgca aaaccttcgg atcgatttcg tgaagccctc
     1141 aatcctttta agaaatccta tcatgatgta ttttatgcgt tgaaaaacgt gaatgtagaa
     1201 ataaaaaaag gtgagatgat cggtttcgtg ggagaaaacg gatcgggaaa atctacaatg
     1261 ttgaaaatca ttacgggagt actgacacca acatccggca cgatggaaat cgaaggaaaa
     1321 atctcagctt tgctagaact aggatcagga ttcaatcctg agtattctgg ctatgaaaat
     1381 atctatttaa atggaatggt tctaggattt tcaagagaag aagtcgatga aatggtcgat
     1441 gatatcattg aatttgctga tattggcgac catatttatc aacctgtaaa aacctattca
     1501 agtgggatgt tcgttcgtct agcttttgct gtagcgatca atgtgaatcc tgatattttg
     1561 attgtggatg aagcattggc agtaggagat cttgaattcc aactgaaatg tatggaaaaa
     1621 tttacagaaa ttaagaactc gggaaaaacg attttattcg tttctcatga tattagttcg
     1681 attcgccgtt tttgtgatcg ttcctattgg ttgaaaaatg gagaagttgt cgaatatggt
     1741 gatacgatgg aagtaacgac gaattacgaa aactttttga agaaaaaatc agttaaaaca
     1801 gtagatcgaa ataaaaacca agtagaacgc ttcgcagctc cagatatcgt agacgtgacg
     1861 aaagccgaat tgttggataa aaataaaaat ccagtggata tgctggaaca aggagaaaaa
     1921 ctatacgtta aagtaaccta tgaagtaaaa gacgatacaa tcaaaaatcc agttttagga
     1981 attgctctgc gtacagtcga caatttttat gtgtgcggac tcaatactct actagatggt
     2041 atcaagatcc cttggaaaaa aggtgagaat atcttttatc tggagtatga aaagttaggt
     2101 ttgttagctg gtgaatatta ttttgacgtt gcggtctttg aagaaaatgc aacggttcct
     2161 ttggtataca agacaaaata catgaacttg tttgtcagcg gttcttatat cggagaaggc
     2221 atcgtggtct tagaccataa atgggaagaa gggactcata gcaatgaaat atgattttga
     2281 aatggaccta gatgaacaaa gcagtgtggg aaaaattgcc gcacagatca aaccaggaag
     2341 taaagtttta gagtttggtc ccggaaacgg tcgattaacg aaacatttga tcggagcaaa
     2401 acaatgtgaa gtgagcattg ttgagctaga taaggaatta ttcgattttg tcagcgaatt
     2461 tgcccaagat ggattttatg gagatatcga aagttttgaa tgggcaaact attatgctgg
     2521 acagacattt gattacgtct tgtttgcgga tgttttagaa catttagtag atcctggaaa
     2581 aacattgaag aaagtcagag aatttttgaa tgaagaagga gagatcctga ttacattccc
     2641 taacttagca cataattctg tcatgatcga tttgttcaat aatcaattgc cttgggcttc
     2701 ttatggtctc ctagatgaaa cccataattc tttttatact catgatggtt tccaaaaagt
     2761 ttttgaaaaa gccggattat tcattaatat tgaagattac ttgtacttgg ctgtaggaga
     2821 tacagaatta aaatcaactt atgaagagtt gccggaagct gtacgctatg atttcaaaat
     2881 gcgaccattt ggtgaagtct accaatactt tttctcattg atgaaacatc cagtagtaca
     2941 aacttccatt gcacagccac aaaactctaa ttatgtaaaa gtactagaag taacccagca
     3001 aacgaaacaa gatgaaacaa tccaacaaat accattcaat aatttcacag gggaaaatga
     3061 gacactaagt ttccccgtgt cagaagcaac agaaagaatg gtgtttcgct tcgcacagca
     3121 acctagcttt atcgaattca gtgcagaagc tgctggagaa aaactcatat ttatcgattc
     3181 aaatgctgta gtcaaaacag taaatgattg ttatttgttt gatgggaaag aaataccaga
     3241 gttcgctcta acggatattt ctgggaaaga agtgactatc cattgtcact atcgctttat
     3301 cggggaattg acaccaacaa tgaaagaatt gctggaaaca attcgtccta tggctgaagt
     3361 cacaaagcag ctttctgaga aaaatgatga attagaaaga gaaaatcagc atcttcttga
     3421 ggaaaataca agactagacc atacgttaaa aacaaccaca aatcgctatt gcacattgtt
     3481 ggaaagtgat gaatggacga tcaaacatag actaggacga cgtaagaaaa aaacatcgaa
     3541 aaaaatacaa gaaaaagagc tttctatatg tattgatgag aaaatatggg atgcagaaac
     3601 aaaaatatta aaaatcatcg gatggggaat cgctaattcg gatcgccagc cattgtccta
     3661 taagttatca gctgaccaat cgccattttt cgaagcaatt cctgtttcac gagatgaagt
     3721 caatcaagca gaacagctcg caaaaggaac agaagcggga tttgaattac gaatcctttg
     3781 cgagcaagaa cgttcattct tagtggaagc agtagctcaa aatggtcaat catggatcat
     3841 tgaaatgtaa ataatgatta acttataaag ataaggagta aacgaatcgt gcaaaaaaaa
     3901 gaaattgccg tatttatcga tcaaatcact cgtgaacggg ccactggcga tttgcttatt
     3961 gtgggctggg caattgatga agtgaccaaa gaaatcccta cgataaaggt agaaaaagaa
     4021 aatgtgattg ctgaagcaac acatgtcgta agattggata tcaatcattt atataatctt
     4081 gatgttaaga cccaaagcgg ttttaagata cgtttatccg ggaaaatgag aggaaaagcg
     4141 atcttagatt ttcaaacggc aaaacatcaa aatggcatag ctgtgaaact aaatggtaaa
     4201 tacccttatg atgacggcat tgaatcttca tgggaaagaa aaaaaagatt actgaaaaaa
     4261 gggataaatt atgcaaggac tcatggcgta aaaaaaacaa tccgccgggt caaactggaa
     4321 ttgaaaccag gatcgatcga ctatgcagag tggatcagtc gtcacgagaa acctgatctg
     4381 aaagagcaaa ggaaagaagc acaagggttt gcataccgtc cattgatttc catacttatg
     4441 cctgtctata acgttgaaat caaatggctc gaaaaatgca ttgattcggt attggatcaa
     4501 acgtatgatc actgggaact atgtatcagt gatgatgctt ctactgatcc agctatccga
     4561 aagtgtctgg aatcctatca agcaaaggat gatagaatca aagttgtttt ccgacaagaa
     4621 aatggacata tcagtctggc aactaattct gcgttggaaa tggcagaagg agaatttata
     4681 gcgctcttag ataatgatga tgaattaccg ccatttgcat tatatgaagt ggcaaaagtg
     4741 ctaaacatac atccagaact ggatttgatc tacagtgacg aagataaaat cgatgcagat
     4801 ggcaatcgct ttgatccgca cttcaaagca gactggtctc ccgatacatt gatgggaaat
     4861 aattatatct ctcacttagg cgtatatcga acaagtattg tgaaaaagct tggcggtttc
     4921 cgtaaaggct acgaaggctc tcaagattat gatttggtcc ttcgtgtgac agaacaaatc
     4981 cctgcagatc atatttacca tattgaccga gtcttgtatc attggcggac gatcccagga
     5041 tcaaccgcaa gtaatggtga agccaaaagt tatatctatg attctggagt gaaagcattg
     5101 acagatgccc tgtctagaag gaatatcaaa ggaagcgtac gtcctggcag aatttctgga
     5161 ttttatgaga ttgcttatga cgtcttgcaa gaggatttgg tcagcgtgat cattccaacg
     5221 aagaatggct atgaagatct aaaaacttgt gtggattcga ttatcgaaaa aacgtcttat
     5281 ccaaattatg aaatcattat tgcagataat ggaagtacag atcctaaaat gcaggaatta
     5341 tttgcagaat acaagcatca gctgaaagac cgatttatcg ttgaattaat tgacattcca
     5401 ttcaattatt cacgtatcaa caatctggca gcagaaaaag caaacggcaa atacttttta
     5461 ttcttgaata acgatacaga agtgattgaa cctgactgga tgacagcgat ggtttcttat
     5521 gcacagtttg atcgaatcgg ctgtgtagga gcaaaactgt tttatccaga tgatacaacg
     5581 cagcatgcag gagtattatt aggaatcggt ggtgttgcag gacatgcatt gaataactat
     5641 gatcgaacac attgtggtta tttcggccga ttggtcattg acgtcaatta cttagcagtc
     5701 actgcagctt gtatgatggt caaggcagct gacttcaatg ctgtcaatgg atttgatgaa
     5761 acattagaag tagcattcaa tgatgttgac ttgtgtttga aagtttatga actgggtcgt
     5821 tacaatgtct atgcacatca agcagaattg tatcatttcg aatctaaatc aagaggttat
     5881 gaagatacac cagaaaaaca aagacgtttt gccggggaaa taaaaaaaat gcaagataag
     5941 tggccttctt atattgctca tgacccattt tataacgata atctaactaa agaaggaatc
     6001 ggcgattttt cattgagacc tgattgatta aaagaactgt attttcattt ttattgatga
     6061 aaatacagtt tttttagtat atgaaaattg taacttgtga ccgaagagat gataggaaga
     6121 acacccattc agtaggaaga atgtgatacg atttttgtcc aactctctgt taggcagaaa
     6181 tatacatata aacatacaat agtcaccttt tctagtttct tttaatttat tttacaattt
     6241 ggttattcac taggttttta aactttgcta tggtaagatg atagaggcat tattttatgg
     6301 ttaggagtgt atatatgaat aaaaatgggg agtggaatgc ggcacgtagg atattcatta
     6361 tcatcatgga tgtacttgta ttgaatgtgt cgatctatct tagctttttg ctgaagttcc
     6421 aaggagaagt cccagcaaga aacttagaga cctttcagca ttcagcagtt tttatttcca
     6481 ttatctttat tgcgttgaat attttgttag gagcttatgt attctatagc cgagtgatca
     6541 gtgatatcgt cttcattact gttattggac aggtgctgat agcattgggg atcatggtag
     6601 ttacttttgc tggacgttgg tttgcatttc cgcgtggcgt catcgtgctc tcgtttatac
     6661 ttggcacgat attacttagc gtttatcgga tagttgttta taaactatac ttaagagtca
     6721 gtcgtgataa gaaagtcctt atcgtaggtt tggagaaaga tgtcgcacca gctatccaaa
     6781 actttcaatc taagaaaaac aacaaacata aagttgaagc agtagtgatc gacaagtatt
     6841 acgaaaacac aaaaaaattg attgatgata tcgatattgt ctacttggct tctaaaatcc
     6901 cagaagacga aaaaatcaat atttatcaat tagtaactaa aaaagagaag aagctatttt
     6961 taaatacaac ttttgaaaat ttaacgatga tcaatccgaa catcatgaat ttcgaagatg
     7021 aaagtatcat tgaagcttcc ggtttcaaaa ttcagccgga atatgaatta ttcaaacgtc
     7081 tttttgatat catcgtttcg cttattttac tgattcttgc ttcacctttt atgctaatca
     7141 cagcgatttt ggtaaaaaca acttcacctg gaccaattat ttataaacaa gtacgtatta
     7201 cgaaaaatca aaaggaattt tcaatctata aatttcgttc catgagtgta acagctgaag
     7261 caaaatcagg accggtcttg gcaaagagca atgatgcacg tgtgacacca gtaggcaaat
     7321 ttatccgtgc cgtacgtttt gacgaattac ctcaaatctt taatgtattg aaaggcgata
     7381 tgtcgatcgt cggaccacgt ccagaaagac catttttcgt tgatcagttc aatgaagaaa
     7441 atccttatta ttatttaaga cacaatgtac gtgcagggat taccggatat gcacaagtat
     7501 acggaaaata tgtatcagat tataatagta aactacgttt tgatttgttg tatatcaaaa
     7561 agtattcctt actattagat ttgaagattt tacttcaaac aatcaaaatc ttatttgata
     7621 agatgtcctc acaaggacta gatgaagatg aaaatatccg ttcgttagca gaaatcgact
     7681 tttcacaaga gaaaatttat cgctagatgg gaaaaatttt gagcaaagaa gtaggagaga
     7741 acattggaaa ataaaaatat aagtatgctg ctttctttat atgataaaga aaaaccagag
     7801 tatttaaagg aagcattaac tagtattttt gagcaaacag taatgccaaa agaaattgta
     7861 ttagtttatg acggccctat aaatgaaaaa cttcaagtag ttgtcactta tttccaacaa
     7921 aaagatcctc acttgtttac agttgtaaaa ttagaaaaaa atcaaggatt ggggattgct
     7981 ttagcaattg gattagaaca tacgaaaaat cgcttagtag ccagaatgga tacagatgat
     8041 atcatggaac cccaacgatt agaaaaacaa ttagagatct tccaaaaaga tccagaaata
     8101 gcgattgttg ggagtaatat tgaggaattt actggcacgc ttagcaatgt tatcggaaga
     8161 aggattgtac ctgaatataa tgatgaaatc cgtgtgtttt cgaaaagaag aaatcccttt
     8221 aatcatatga cagtcatgtt tgataaaaat gctattttag aagtgggaaa ctatcgccca
     8281 ttaattggat ttgaagatta ttatttgtgg gtaagactgt tgaaggcggg atataaaggg
     8341 tataatatcc aagagattct agtccatgct agagcaggtg ctgacatgta tgctagacgt
     8401 ggaggaagta aatatctttt accaggaatt aaaggccgat atcgcgtatg gaaagatggt
     8461 ctaggatcac taaaagattt tctgatagtt atttgcggac atcttgtaat tagtttatta
     8521 ccgaataaaa ttaggggaaa agtatatgaa tcaaaactta gaaaaacaaa ctaaactttc
     8581 tataatcata cctttttaca atgcaaaaaa tactttaaaa agagctgtag acagcgtctt
     8641 aacgcaaggt tatcccaata ttgaaatctt gttaattgat gatggatcta cagatggtag
     8701 tagtgacatt ggtgacgaaa ttgctaatct agagagtaaa gtagcatgtt atcacttgga
     8761 aaacgctggt ccatcagttg ctaggaattg tgggataaaa gaagcaactg gaaatttggt
     8821 aggattttta gatagtgacg attatttttt acctgattgt tttaataatg ttttgaaaga
     8881 ctaccatagg aattaccccg atatcattgc ttttggatta agtaaaggaa aaaatataga
     8941 aaatgcaaat ttatttgttc ccgaaaagaa gattggttgt tcaagtgaag aagcaattga
     9001 gcaaatgttt agaagtaagg cagtggattt ttacgtatgg aataaatttt ttaaaaggga
     9061 attattcaat catatagttt ttccagaaaa taaattatat gaggatatgg ttcctatgta
     9121 tgaagctttt agagcggcta gtaaaattga tattttgcca ttttcaggta ttttttatta
     9181 tcaaaataat gatagcatcg tttatcagtc ttttaattca aaacagtatg ataatatttt
     9241 gcaaagaaaa atattagttg atgaagtaaa gaaagattat cctcgactta ttaatttggc
     9301 caatgctcga ttagtagacg gatacctttc aacagggttt aaaattactg gcaagaatca
     9361 aaatcagaaa gaaaagcaaa aatattataa tcagtctaaa agagaaataa aaagtatata
     9421 ttcaaaaata agaagtaaca atgagatatc aattgctaaa aaattagctt tatttttata
     9481 cttaattaat cctaaaactt attttatttt atataaaaaa gttctaaaaa aataaagttt
     9541 ttttcgatat gttatttttt atgtttatta acttcggaag actaaaattg ttactggtaa
     9601 tgatacattt gtaaaaataa aaaagatgat ttttaggaag tgaagagagt gcataaaatt
     9661 agtgttatag tacccgcata taacgtagaa gactatttag aaaactgctt agaatctatt
     9721 tccaaccaga cacattcaaa tattgaaata atagtagtca atgatggttc cccggatcga
     9781 agtggggaaa ttgcagaaga atataaaaag aaagatatgc gattaaaagt gatacatcaa
     9841 gtgaatggtg gtttaagtgc ggctaggaat tctggtcttg attctgcaac aggagatttt
     9901 gtgtgcttta ttgacagcga tgattggata gaaccagact atcttgagtt actatatttt
     9961 ggcacgcaaa cttatggagc agatatctcg gtgctaaaaa taaaaaagat ttcaagttta
    10021 gaacaactaa aagaacatac aattgctgag agaaaatggg aaatatttga tagagaacaa
    10081 gcaatgaaaa acttatttat aaataataaa attggctatt ctgcaaataa taaattattc
    10141 aatagaaatt tatttgataa tataagattc ccactgggta aattaatgga agataaggct
    10201 actacttatt tgctgatcga tcattccgaa aaagttgctg tcaattcatc tgctaaatat
    10261 cactattacc aaagccctaa aagtatactg agaggaagct tcaatcctag aatttttgat
    10321 tcttttgaag tccatgaaga tatactaata ttcatagata agttttatcc ggaattatca
    10381 attaatgtta gaactcgcta tgtctatgaa gcaataagga tgatgatgac aatgattaaa
    10441 aataactata ccaataaaga agattattta cgttctataa atattttaca acgctttgaa
    10501 aaagaagtga aaattgatcc cgacttttca ccaaaattaa aaaaattagt aaagttttta
    10561 ttaataaata aaaacttgcc ttactatatg tcaagtagta agatagcaag taaattcttg
    10621 attaagatag aatctgtgag gtaagagatg aagaatataa aaaaattttt ttcaaggaat
    10681 attttcccgg tttttattaa aatattacct attcaaaaaa atttaattgt attttcaagt
    10741 tttggcgggc gtaactattc tggaaatcca agagttattt cagagaaaat tcatgaaaaa
    10801 aaattgaatt acagaattat ttggttaatc aaaaaagaag cgattataac tgatttacca
    10861 gattatatta aaagaattga ctccgattct tttttagctg ccttttattt atatacagct
    10921 aagtattgga tagacgatag tcggaaaacg attaagtatg caaaaaggaa aaaacaactt
    10981 tattttcaaa catggcacgg aacgccttta aaaaaaattg aatttgatgc aaaagataag
    11041 ttacctaaac gttacctaag ctatgcgaaa aaagatagtg agtcaattac atatttatta
    11101 tctggtaatc gctatagttc taagaagtat gtttcagcat tcaatatcgt tccttctaaa
    11161 atattagaag taggaactcc tagaaacgat gaattagcct ctagtaaaga gcaagtgttt
    11221 gacttgaaaa agaaacaaat caatgtctta tttgctccaa cttttcgaaa taatattgag
    11281 gacaacggaa ttacgcaact agaatggtta ggtgtggata acttacgtga attttttaaa
    11341 aagcaacaac aagaattaaa ttttatgact aaatttcatc ctaatgttca tagtaaatta
    11401 gcaactgatt ctaaaagtat agaatatatg aaaaaacatc aaattacttt aattaatgat
    11461 gggattcctc ttgaaatgct ttttgaagag atagatgttt taatcacaga ttattcatct
    11521 atttttttcg attttgcgct aacaaaaaaa ccaattattc tttttaacta tgatgaaaaa
    11581 gaatatagct tagaaagagg tttttatata cagttaagtg agttaccaat tataagcgta
    11641 caagatagtt atggccttgt tgatgttttt aaaaaccaac aagaaaagct tttgaaaagt
    11701 tctcaagagt tacttgatta tattggaaat tatgaaacag gagcaagtac agaaaaggtt
    11761 cttgagttga tagagaggga tataaagtga aaaaaacagt aaaagatata ttttataatg
    11821 cgatatatca agtctttttg attgttttac ccttacttac tatacctata cttagtcgta
    11881 gaattggttc aactggttta gggatttatg ggtatgtttt ctcaatatca cagtttttaa
    11941 tgactgtcat tgctgtggga atgaatcctt ttcgtattcg aaatatcgct aaatcaagga
    12001 aagataaaaa agcactatct ttacaattct ggaatattta ctttattcaa tttttaatag
    12061 gcttgagtgt ttcctttctc tatatagcct atatttattt ttttgatgta aagtataaaa
    12121 atttatactt aattcaattg atatttattg tagggctaac attagatatt tcttggtttt
    12181 ttcaaggaat tgaagaattt tcgaaagtgg ttattagaaa tacagttatc aaattatcat
    12241 cggtattgtt aataatcatt tttgtttcaa ctaaagatga tttgtggatt tacgtgttca
    12301 ttacttcaat tactaacttt ttaggaagtt tagttttctg gttaagtatc cacggaaaag
    12361 taggtaaacc aatctttaac tctaatgttt ttaaacagtt gtggaaacca gcttttatta
    12421 ttttgactcc tcagttgttt atgcaagtgt acacaacttt agacaaaacg atagttgggt
    12481 attttgtgga tccaacggaa ttatcttatt atgatcagtc tcaaaaaatt gctcgtatta
    12541 tattggcagt cttaacatca attactattg ttatgttgcc taaaatatct aaagcacagg
    12601 cagaggggaa atcagaaaat attttagttt acacaaaaaa atcttttgat tacacgatta
    12661 tcattgcatt aattcttttt agtgtggtgt ttatcaatac aaaagaattt gttccatggt
    12721 tttttgggga gaatttcaaa ccgatgacaa taaacatgct tatttcttgt ctaattatag
    12781 ttgttagtcc aattggtgga gtttttgcta accaatttgc aattgctata gaaaaagata
    12841 aggagtatgc ttatccaatg atcattggtg cgattgtaag tattattgga aatgtagctt
    12901 ttgtgcctac ttatggcgct gtagcggcta cgatcgtact cgtggtagta gaattattag
    12961 tctttttctt tagagtattt tttgtacgaa aagaaataaa aatatcctat ttctttggga
    13021 agaacgttat cttatttttt atagtcaatc ttactttgac tacttgggcc tattactttc
    13081 ttccacaatt attctcttct tcatttttag atatggcaat taaatcaata atgattttca
    13141 ttatttatct tccgtttgtt tattttcttt ttccagatat acgaaatgaa ataagaaaga
    13201 ttactcgaaa ataaaataat gttgtaaaaa agtagttctt tgtcaaatcg ggctgataag
    13261 gtcatttatg aacaacaaag ttagtaagag ttactagctt ttaatgcata gctgattaac
    13321 ctgttgatta gaaaaatcca caagcgcata ttggtaaaac tgcgaaaccc ataagcaaat
    13381 cgtttcaatg ttttgatatg tgtgcgaaga ttctctaatt ttgttattag aatacttttt
    13441 cgaaaatgct agttaaaagc ctttttcaga tatgaaaaag gctttttgag atagtgaagt
    13501 ttcgagggaa cttctttggt cgtttctcga agtagctagc tgtttcttgt gataattgct
    13561 aagcagcttt tgataaactg cttagcaatt ttaagttcat aagagattga gggtagacga
    13621 tcaactactt cactttctgt taaatagcgc cattgaaaat tctgccattt cttgaatgtt
    13681 gtgtaatcca actcgtcatc tgattttaga agtaatttat acaaaggttt caattgtcga
    13741 tagtacttag cttgtgaagt gtctttttga ttcaactgct tcatcacacg ttttcgaaca
    13801 cgatcgaaaa acttgttcaa gtgttgtcca atgtgaaagc aatcaatgat gatttgggcg
    13861 tttggaaagg actctttgac taatgaaaaa taaggggcat tcatatttgt gacaatgtat
    13921 cgaaccccca tatgagcttt tcttgtaaaa cgcatgaaat aagcaatcaa atcttttttt
    13981 ctccggctgg acaaaatgtc aaagagcttc cctgtctctc tattcataca agaaaaggag
    14041 aactttccat gggaaattga aagagagcga aactcgtcga atagtaaaca atgaggtaaa
    14101 tagtctagca tctgtcgcag cattgccgat agagcagata atgtccgttg aaatgtcgtt
    14161 actgaaagat cacaacgttt cgtaagtgtc tttaacgctt gacgctcggt caactcttct
    14221 aatatcgtgt aatgaacttg tttcgaaata aaacatgaag gaacgacaag atacgtatga
    14281 gcagtaaaat attttccgca tgattggtag tggtaatgtt gttttctaag acgcaaatag
    14341 gtttatttat tcccactgcg atttaagata atcattgaaa tcttcttgcc gtttttcacg
    14401 agctgcttcg cagtttcttt acaaaataga caatgtctaa aagtgcgaac taatgtacct
    14461 tcgattacga tggctttctt gtccttataa aaagtttttt gaaaagaatt aatttcaata
    14521 tttttatctt taatagtgaa gtgctctttg atagaacgag aaatggatat acaaaatctt
    14581 cctttcagat tgatgtttta gacgactcta ttttatcgaa atttttgtat gtctgttttt
    14641 tttacaagaa aatctaggat cgacagaaaa ttctatcagt cctagaaatt atagagccat
    14701 ttttttacgt cttatccaga tatattgttc tttcattgtt taaaaaatat tatttatcac
    14761 tatttaaaga attaagttcg ttctttattt gagttgtaga gatctcaggt gttcttggta
    14821 aataaattac ttgtgcatct gttagttgtt caagttcatc aaattgtcca gcccaatcat
    14881 cgcccatcac gaaataatct gcatggtatt cttttatatc ttttactttt tgttcccaat
    14941 tttcttcagg aataactaaa tcaacgtagc gaatagcttc aagtaactgt tttctttttt
    15001 cataactaaa ataacatttt ttttgtttcg cattccaatt gaattcatct gtagaaaggg
    15061 caacaataag ataatctcca tattgttttg ctcgtttcag taaattgata tgcccgtagt
    15121 gaagcaaatc aaaggtaccg taagtaatta ctcttttcat ctttcacctc tcgaaattca
    15181 ttaatataca ttgttagttt agcatttttt tgttagaatt gaaagaacca gtttatccta
    15241 aaaaggataa gaaatgttta tgagaaatag gtggatggag gaaaaatgtt gaatgaaaaa
    15301 tttttaaaat atgtgaaagg ttggtatttt atttattttg gttttattct aatgttacga
    15361 gcaataggcc cgtatttact attacctact agatatgata ctttactttt ttggcctgct
    15421 ggaatagcgg gagttgtctt gatcattata gatgcaatta ttgcatttag agaaaaaaat
    15481 cttaaaaaat atgattggct attaattgct tttattggcg cgatgctagt aagcagttta
    15541 atgaatttaa aatatggata tagcgaaaat atcaagttaa tcatgtggca attaatcttt
    15601 ttctttatga tttatcagtt tggcaaagac tacagtagta actttttttt caaagtattt
    15661 acttggatac tttcattggt atggttttta gctaatttag tatcattata ctggtttgtc
    15721 attcaatatg gcataaaaat tccaataaaa tataagtatt acccagttcg tttagggtgg
    15781 ttaggcaacc gtttatttgg tgtatataca gatccaaatt ttggtgcggt tatatcatta
    15841 attgtcatca ttattgctat atattatttg gtcacttaca agcatttaaa tcttctattt
    15901 aaaatatttt ttgcaattaa tatttttatt caattctcgt ttattgcttt atcgggctca
    15961 cgcaccacat tagtttgttt attaatagca acttttgttt ttagtttctt tacaagttat
    16021 agtagtcgtt ttcttaaaac taatacttta aaaagatggc taactgcatt agtagtagga
    16081 ttgctaagta taattgcgat gtattatgta acagatataa ctgctcaagc atgggcacat
    16141 gttccgcata ttacagaaat aaaaaaagat atatctgatt catcaggaac aactagcagc
    16201 aataaagttg atttaaaacg ccctgacact gatggcgggg atgtttctaa tttaaggttt
    16261 ggactttgga aaagtgcaat tgaaatattc aagagtaagc cgatatccgg cggttctcca
    16321 ggatactata ttgaatacgc acatgataaa ctaccatata cattaatggg aaaagatact
    16381 ttaactgctc ataattttat tttcttagtt atggctgcta caggaggatt aggtctattg
    16441 acattgttaa ccttcttatt tatccaatgc tttaaaactt tgcgttattg tttcacacaa
    16501 ccaaatctag taaaagataa gcactttttt gctattttaa tgattcttac aatagcagtt
    16561 tcggcattat ttatcacaga actcattttg gttagtacga taggttcatt tattttttgg
    16621 ggatatctgg gttctatcca aaaaactata tctaaagaag aaggagtaac taattgaaac
    16681 tatttttagg tatataaagt agaaaagaaa actagttatc ccaacttcaa caaattttat
    16741 agtttttaca atataaaaat ggtaaactaa ctttattaag tatgagaatg cataattgga
    16801 gtgaatttga tggatactat attgattact ggtggagctg gttttatcgg ttcaacgcta
    16861 gcgaattatt tgggaaaaga aaacaaaatc gtagtagtcg acgatttatc aatgggaaaa
    16921 aaagaaaact tggatatgga gaaacaagtg actttcattg agggggatgt atcagatcct
    16981 caattgatgg aaagaatcat gaaacagtat cagtttgcct atatttttca tttagctgct
    17041 gtagctagtg tggcagattc tgtagaacgt cctctagaaa ctcatcgtgt aaattttgac
    17101 agtgcgcttt tattgcttga attggtacga aaatatcaat ctggtttaaa acgattggta
    17161 tttagttcat cagctgcagt ttatggcgat gaaccaacgt tgccaaaaaa agaagaatcg
    17221 gtcatccgtc ctttaacacc atatgccatc gataagtttg catcagaaca atatgttttg
    17281 gattactgtc atttatatga tgttccgact agtgctgtcc gattttttaa cgtttatggt
    17341 ccaaatcaaa atcctaactc tccatatagc ggagttattt cgattctagt ggaccgatac
    17401 aaaaaacaat tggcaggaga aaaaacagaa tttactttat ttggtgatgg tagccaatct
    17461 agagacttcg tttatataga agatgttata caagcacttt tacttgttgc taaggaagaa
    17521 aaagctttag gacagcaatt caatgtcggt acaggaaaaa gcactacgct acttgaattg
    17581 attcattcaa ttgatcaaat cttaggaaca gagctagcgc ttaaatatga agcggagcgt
    17641 tcaggggata ttcgtgattc tttagcggat atttcaaaga tccgttcttt aggttatcaa
    17701 cccaaatttg atatattgaa cggaatggaa cgttatttaa agacagaaat cgattgaaac
    17761 aaa
//
LOCUS       DAFEPQ010000053        17627 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.055, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000053
VERSION     DAFEPQ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.055"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            287..694
                     /locus_tag="LP543_001920"
     CDS             287..694
                     /locus_tag="LP543_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322084.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="LP543_001921"
     CDS             708..1109
                     /locus_tag="LP543_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322085.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="LP543_001922"
     CDS             1111..1482
                     /locus_tag="LP543_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322086.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1820)
                     /locus_tag="LP543_001923"
     CDS             complement(1518..1820)
                     /locus_tag="LP543_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322087.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2069..2269)
                     /locus_tag="LP543_001924"
     CDS             complement(2069..2269)
                     /locus_tag="LP543_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL2322088.1"
                     /translation="MKINYTVDLVSFSLVINFCLFSLRLFIEGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(2574..3806)
                     /locus_tag="LP543_001925"
     CDS             complement(2574..3806)
                     /locus_tag="LP543_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBL2322089.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSAAGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVIDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            4063..4632
                     /locus_tag="LP543_001926"
     CDS             4063..4632
                     /locus_tag="LP543_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL2322090.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(4810..5250)
                     /locus_tag="LP543_001927"
     CDS             complement(4810..5250)
                     /locus_tag="LP543_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBL2322091.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLSGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            5408..6172
                     /gene="map"
                     /locus_tag="LP543_001928"
     CDS             5408..6172
                     /gene="map"
                     /locus_tag="LP543_001928"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBL2322092.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            6204..7127
                     /locus_tag="LP543_001929"
     CDS             6204..7127
                     /locus_tag="LP543_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBL2322093.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            7203..8342
                     /locus_tag="LP543_001930"
     CDS             7203..8342
                     /locus_tag="LP543_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBL2322094.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            8335..9135
                     /locus_tag="LP543_001931"
     CDS             8335..9135
                     /locus_tag="LP543_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL2322095.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            9135..9962
                     /locus_tag="LP543_001932"
     CDS             9135..9962
                     /locus_tag="LP543_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL2322096.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            9940..10674
                     /locus_tag="LP543_001933"
     CDS             9940..10674
                     /locus_tag="LP543_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL2322097.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            10774..11640
                     /gene="rfbA"
                     /locus_tag="LP543_001934"
     CDS             10774..11640
                     /gene="rfbA"
                     /locus_tag="LP543_001934"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBL2322098.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            11654..12226
                     /gene="rfbC"
                     /locus_tag="LP543_001935"
     CDS             11654..12226
                     /gene="rfbC"
                     /locus_tag="LP543_001935"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBL2322099.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            12248..13276
                     /gene="rfbB"
                     /locus_tag="LP543_001936"
     CDS             12248..13276
                     /gene="rfbB"
                     /locus_tag="LP543_001936"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBL2322100.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            13374..14225
                     /gene="rfbD"
                     /locus_tag="LP543_001937"
     CDS             13374..14225
                     /gene="rfbD"
                     /locus_tag="LP543_001937"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBL2322101.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            14259..16292
                     /locus_tag="LP543_001938"
     CDS             14259..16292
                     /locus_tag="LP543_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322102.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            16336..17616
                     /locus_tag="LP543_001939"
     CDS             16336..17616
                     /locus_tag="LP543_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBL2322103.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYGEQPDSAHSSRIQRYH"
BASE COUNT         5531 a         2966 c         3590 g         5540 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaattc
     1741 catactttat aaaaatcttt gataaatcta ttcgaagggt caaatttaaa ccataattgt
     1801 gtttttcctt ccatcagcat aaaagttcac ctcaaaaaga gtatacgaac gaatgttctt
     1861 tttgtgaagg cggaatttaa tatactgaat aatagaaaac aagtacttgt gccaaattat
     1921 gtgccaaaaa aattcgaatt caacaaaatg taaacaaata gaaaaccttc tatattaaga
     1981 tttcctgcaa taacaaacac aacagaagac gtgtaatagt tagtcaggaa cgtacaaata
     2041 acccctgtat cctttgcggt acagggggtt attttgtata agtagacatt gtagtttcta
     2101 aactgctatg tcctaaacgt ttcgatacgc taagtatatt tactccttga taaagtaaaa
     2161 tagatgcatg cgtatgtctt agtccctcaa taaacagtcg aagagaaaat aggcaaaaat
     2221 tgattactaa agaaaaactt actaggtcga cggtgtagtt gattttcata cttcaacaga
     2281 ttcgaattaa acgatttcga ttggcttata ccagtcctat ccgacgaaag atatatcgtt
     2341 cttaactcta gcggtataac ttgaagcttt acgtaaagcc aatgggaatg aactggagat
     2401 gactttttat tatttttagt aatagctgat gggttaatac ttacaacttt ccttttcaaa
     2461 gtaccatata aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt
     2521 agatccttta ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc
     2581 caatcgccat ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc
     2641 atcttgtcag atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct
     2701 gctaattctt catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct
     2761 agtgctaagt gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat
     2821 tgagaaatag gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca
     2881 tctgtatcaa gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaatgact
     2941 tttccatctt taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc
     3001 tttgtacttg aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc
     3061 gtcggcatat ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa
     3121 agatggtttt taggcaatcc gataatgata tctgtaccgg gagcagtata gtgcagggca
     3181 gaaaattgtt ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc
     3241 catgctaaga cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat
     3301 gcctcaactt gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca
     3361 gccgcagcca cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt
     3421 agtgctttgc cagccgctga ttgataagac gctactcgat ggctatctac accagcaaat
     3481 gcatcgggat cggcagaaac aacactgatc ctgcttgctc ctttttcaag ccattcatcc
     3541 gcttgatcga ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga
     3601 aattctcttt ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta
     3661 taagcttctt gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca
     3721 actgtgtgtc ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt
     3781 agaagttcat taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa
     3841 ctattagcat gataccattt aattatgatt caaacaatga ggcctttaaa taaaaaaaag
     3901 aggttatttt tttaaggtta agtttcatga tatatgttac tacttgctat ttcagcttgt
     3961 gaagaaaatc aaaaactaat tttgcatatc aaaaaaaaca tgttacaatg aaattaatca
     4021 atggtggaag cgttataaaa aaattaagga gattaaaaaa atatggcaag aaaaaagacg
     4081 attactagag aacagatttt ggcagctgct tatgaagtag ctgccaccga tggatttaca
     4141 cgatttactg cgagaaacat cgcgaacaaa atgaattgtt ccacgcaacc gatttatctt
     4201 gagtttaaaa atatggatga cttgagacaa gctctttttg agcagatcta tgatcattta
     4261 caatatgaag tgttcactgt tgagcataca gggaatacga ttatcgatct tgcttttaat
     4321 tatatccatt ttgcaagaag agagaagaag ttgtatcgtt cactgtactt agaagaatat
     4381 ggtggaggaa aagagatgca gaatttctct tatacttact ttgtgaatac agtgaaacgc
     4441 gatccagctt atgtgaatct ttcagaagag cagatcgaat ctttgtataa tgggacatgg
     4501 atcatagcta ctgggatcgc agctttgatg tcttcaagca tcatccatcc aactgatgaa
     4561 cagattgaaa agatgataca agacagtata gatgctatct tgaaattcaa agaaccaata
     4621 gaaatcgatt gaatatactc aaaagttcca caaatcagta gataacaaga gatgtgtcaa
     4681 aataaggaaa agttcgtttt tcttacagcg tatgcatttt taactcccga agaactcttc
     4741 catgaatgca atgaataaaa gagccgtaca gattgaataa atctggatgg ctcttttatt
     4801 gcaagtatat taaatttttg cgactagttc tttagcgaaa gcttctaaat tttcgatatc
     4861 ttcttcttca gcattcagat ccactttaac attttcggct ccttttgatg cgcctgtttt
     4921 tgcaaaagca gcatcgaaat catccaccga tttgcagaat tcatcgtaaa aagtatcacc
     4981 agatccgcag acaccgtaaa tttttcccga tagatccaat tcttgcaagt cttcataaaa
     5041 gtctacgatc tcatcaggca gatcgccatc atcgtatgta taagtagcta ctacgcaaat
     5101 atctgcctcc tcgaattcat ctgcatctac ttgggtacat tcgttgattt cgacttcgat
     5161 atccagattt tcaaatgctt ctgcaacaat atcggcgatt tcttcagtgt ttcctgtcat
     5221 gctagcatat acgatttttg ctaaagccat gatgtgcctc ctcgtttata attatttaac
     5281 aattatgaat tataccaaaa catcattgaa aagaaaataa aacctagaga cacctgggga
     5341 atgtcatttg gctaaatgta tgccgctatg ttaaaatgag agggatttgt aaaaaggaga
     5401 cgaaaatatg attactttaa aatcagcacg tgaaattgaa atgatggcag aatctggagc
     5461 tttattagca gatgtacata aaaatctgcg tgattttata aaaccaggaa tcacaagttg
     5521 ggatattgag gtatttgtca gaaactatat cgagagtcat ggcggaattg ctgcgcaaat
     5581 tggctttgaa gggtatgaat atgcgacttg tatcagtata aatgatgaaa tttgtcatgg
     5641 attccctcgt aaaaagcctt tgaaaaacgg tgatttggta aaagtcgata tgtgtatcga
     5701 tttgaaaggc ggcatttctg attcttgttg ggcctatgta gttggagaat ctacacctga
     5761 gatcgaccga ttgatggaag taacgaagaa agctttgtac atcggtattg agcaagcaca
     5821 agtagggaat cggattggtg acatcggtca tgctatccaa acttatgtag aaggcgaaaa
     5881 tctagcgatc gttcgtgatt ttattggtca tggcgttggt cctacgattc atgaagaacc
     5941 agcagttcct cattacggag aagctggaaa agggttgcgg ttgaaagaag gcatggttat
     6001 cacgatcgaa cctatggtta ataccggaac ttggaaaatg aaaatggatc ctaacggctg
     6061 gactgcgtat acaagagacg gcggactttc ttgccaatat gaacatacat tggctattac
     6121 taaagatggt ccaagaattt tgacatcaca aggagaagaa ggcacttatt gatctatcca
     6181 gtcaataggt acgggagagg tacatgaaag tttggaacaa aataaaacaa aatgaaaatc
     6241 tgatgcgctt tattgagacg actcaaaagc gcatcactga ttcagaaatg agtacgacct
     6301 ctgtcgtcgt tgcttattat ttactcttgt ctttatttcc attgattatt gcttttggta
     6361 atattttacc gtttctccac atcgatcaag agactgtact gacctattta cgagaagtga
     6421 ttcctgagac cgtttaccag tttatcggtc ctgcaatcaa agaccttttg acacaaagtt
     6481 caggaggctt gttatccgtt tctgcattag cggctttatg gtctgcaagt caaagtatca
     6541 atgctttgca aattgcgatg aataaagcgt atggcgtaga aaatagaaaa aattttttga
     6601 tcgtccgttt cttctcacta attgtcattg tcttgttcat gattgctatt agtggggtaa
     6661 ctatcgttct tgggttggga cagctgattt tggatgctat tcagccaatc gtacaaatac
     6721 ccgtgaactt tatcgatcag ttccaaactt tgaagtggcc aatcacattg gtagctttat
     6781 ttgtcatcat gttcttgatt tatttaattg tccctaatgc tcagttgaag ttgaagacga
     6841 tcattccggg cacgatcttc gctactgtcg gctggatgct gttatcacaa gtgtttagta
     6901 tctatgctag gtattttgca acgaggatca gcggttatca gatcatagga agttttattg
     6961 tactcatgct ttggcttaat tttgctgcta cgatcatcat tttgggcgga atcatcaatg
     7021 cagttgttca agaatacgtg acgggtcatc aaatcaaaaa acgtcgagat ccaagccgaa
     7081 aatggttcaa taaattgaaa actaaatttt ctcataaaaa aaagtagaaa caagagttct
     7141 tattgtaaat ttatatcagt aatttataat aattatgact cttaattaaa gtaggtgccc
     7201 atatgcttgt ttcttttagt atggttataa gattttttct gacaatttta ttatcactga
     7261 tattaacacc tgtattcaaa atcatttcag tacaaacagg tatggtcgac aaaccaaatg
     7321 aaagaagaat aaataaagtt ccaatgcctt cagcgggcgg actgcctatc tttatttcat
     7381 ttgttatatc gactttattc ttattccgta aaattatccc tcaatcgtat attgtaccga
     7441 tcttgattgc ttctttagta atcatagtca caggagtgct cgatgataaa tatgagctga
     7501 cacctaaaca aaaaagtata gggatcttga ttgcttctct tattatttat tttgtagcag
     7561 atatacgtat tgattccttc acgcttcctt ttattggata tatccaacta ggctggctta
     7621 gttttcccgt aacgattttt tggatcttcg gtatcacgaa tgcggtcaat ctgattgacg
     7681 gtttggatgg gctggcggca gggatttcat tgatcggttt aattacgata ggaatcattg
     7741 gatatttttt cctccatgct tctacagtgt atattccagt tgttattttc tgtcttgtag
     7801 caagtattat cggttttttt ccttacaatt tttatccggc aaagatttat ttaggcgata
     7861 caggagctct attcctaggg tttatgatgg ccgtcctttc actacaaggg ctgaagaacg
     7921 tgacttttgt ttcatcgatt tctttgctga tcgtcatggg agtgccggta acagacacgt
     7981 tttttgctat cattaggaga aaagcaaacc gtgtttcttt ttctacagca gataaaaaac
     8041 atcttcatca ccgactaatc agtttaggct ttactcataa aggtgctgtc ctgacgattt
     8101 atgcgatggc tttgatgttt tctttcacag ctatggtcat gaattatact ggaagaatcg
     8161 gaacgatcat attgattatt gccatgttgt ttgctgcaat tttattattt gaattgattg
     8221 gtctgatcaa tgagaaacat cagtttattt taaaaacatt gcgattttta gggaataaag
     8281 agtaccgtac gcaagtaaaa cagaaatatt tgaagaaatg gaaccgtcat atagatgaat
     8341 aacaaattaa accagccgat ccttattagt attgtgacgg caaacagtga taaaattttt
     8401 cggacactag ataattttat ttcaactgta aaagatgaaa cagcagtaaa aatccgaatt
     8461 tttgacaaca attcacttcc cgagtacaaa aaaagactaa cagagtatag gaaatatccc
     8521 tacatcgata tctattttca tgatgaaaat tccggatttg gttatggaca taatcacaat
     8581 ctgatagcta gcgattctcg ctatggcatt atttgtaacc cagatatttt agtggatgaa
     8641 gagacagtta cagcattggt cttgttattg aaagaacatc cggaatgtgc gatgttagca
     8701 cctaagattc ttaatgaaga tggaacaact cagcacctca tccgtgagca gctggctgtt
     8761 tttgattacg ttttgcgctt tgtgcctttc aaatgggtca aaaaaaagtt tgacaaacga
     8821 ctggctgtgt ttgaatgccg aggattgcct actgatgcta atagttatgt acggatgatc
     8881 tcaggtagtt ttatggtagc tgatattgat aaatttaaag agataaaggg gtttgatgag
     8941 cgtttcttca tgtactttga agacaacgac ttgtgtttga gttttgaaaa agcaggttac
     9001 aagttattgt acacaccctt ttatgcagtt atccattttt acgggaaggg tgcgcatcga
     9061 aatgcaaaac tgtttcgcgt gtttttgact tctatgatga aatttttcaa taagtggggg
     9121 tggcgttttt tctgatgaaa ccagaaatat tagtagtcgt tgtgctttat cagcaactat
     9181 tcagtcaatc tccagcttat ggaccattaa gcaaagcact tagcgaaaag caaattcagc
     9241 ttattattta cgataatagt cctcaaaggc agcaaaatga gctgtttgaa aaagaaaata
     9301 tcgtttatca tcatgatccg caaaatcctg gtttagcggt ggcttataat tttgcgttgt
     9361 ctcaagcaga tgaaggaaca cgttgtctcg tcactctgga tcaggatacg aggctagtcg
     9421 acgattattt tgaactattg cgtaaagtaa cctttacaga tgaatgtgtt gctgcagttc
     9481 cgatgatttt ttctgggagc cgacaagttt ctcccctata ttcagatcgt tatatcaatc
     9541 gtacagctca agcagtagaa gttgaaggcc caacagctga acgaatcatg gcaatcaatt
     9601 caggtgttgc ctggtctgtc aaatttctaa aaaagattgg cgggtttaat cctgcttttt
     9661 ctttagattt tttggatcat tggctgtttt ggaaagtgga tcagttggaa aaaactgtgg
     9721 agatccttcc tgtacgaatg gaacatgatc tttcggtttt agactatgaa aaagtttcaa
     9781 gtaatcgata ccgttctatt ttacaagcag aaaatcgatt ttatcatgaa tatgatcaaa
     9841 ggcatttatc cagtcaccga cgacaattat tgttaagaac agctaaacag ttcttgaccg
     9901 tcaaaaaccg gcatatctgg cggatgactt tgcgctcata tgttaagaat tggaaggtgt
     9961 gaattcgtgc gttcggtttg tatagctacc tttaatggag gcagatattt aagagagcag
    10021 ttagatagta ttttatctca gctttcagca gaagatgaag tagtcatttc tgatgatggg
    10081 tcagtagatg atacatggga tatattaact gaatacgcag aaaaagataa gcgaatccag
    10141 cttttttcgg ggcctcgtca aggattgatc gctaattttg gttatgcgat ccagcagacg
    10201 aaaggagatc taatcttttt ggcagaccaa gatgatgttt ggttggagac aaaagtgaaa
    10261 aagatcactg actattttga gcagaatccg gatcagctag tagtagtttc tgatttaatc
    10321 attgtggaca gcgagctgca agagatccat gattcgtatt tttcgtttcg aaattccaaa
    10381 gaaggatggc ttaataatgt gataagaagt cactatattg gtgctggtat ggctttccgg
    10441 tcttctttaa aacccgatat tttgccgatc ccgtcagaag tgccgatgca tgatatgtgg
    10501 attggtttgc tgggaggaaa gaagactggg ttcctaagag aaccgttaac tctgtaccga
    10561 agacatgatt taaacgcgag tgagattgaa acacattcta aattacggca aaaaatttct
    10621 tggcgtgttc atctgattac ggcgttgatc aaacgaaaat ggtttaaacg ttgataaaag
    10681 atgtttaaat ccttaatatt tccatgacaa atataacaat tgatgagata ataacaaagt
    10741 gaaatttgaa atcaaaaagg agagcagata tccatgaagg gaatcattct tgctgggggc
    10801 agcggaacac gtctgtaccc tttaacaaag gcaacatcga aacaattgat gccgatttat
    10861 gacaaaccaa tgatttatta tccaatgtct actctgatgt tggcgggaat caaagaaatt
    10921 ctgattattt caacgccaca agatacacca cgtttcaagg aattatttgg aaacggaaat
    10981 gatttaggac tgcagatcga atatgcagta caagaaagtc cagatggatt ggcacaagcg
    11041 tttatcattg gagaagaatt tattggagat gatagtgtct gcttggtatt aggggacaat
    11101 atttactatg gtggcggtct gtctaaaatg ttgcaacgag cagcttcaaa agatacaggc
    11161 gcgacagtat ttggctatca tgtcaatgat ccagaacgct ttggtgtagt agaatttgat
    11221 gagaatatgc aagctttgtc gattgaagaa aagcctgctc agccaaaatc aaattacgct
    11281 gtaactggcc tttattttta tgacaatgag gtaatttcta tcgctaaagg tatcaaacct
    11341 tcagaacgtg gagaactaga gattacggat gtcaataaag catatttgga taaaggaaaa
    11401 ctatccgtag aacttatggg acgcggattt gcttggcttg atacaggaac acatgaatca
    11461 ttgcttgaag catctacctt tatcgaaaca attgaaaaac gtcagaactt aaaagtggct
    11521 tgtttggaag aaattgctta ccgtatgggc tacatcgata aggaacaatt gctggccctt
    11581 gcacagccat tgaaaaaaaa tcaatacggt caatatttat tgaatttggc cgctgaataa
    11641 ggagtcctta cagatgaaag taacagatac aaaattaaca gatgtcaaaa tcgtagaaat
    11701 ggacgtcttt ggcgatcatc gcggtttctt cacagaaagt tattcaaaag caaaatttgc
    11761 agaacatgga ttgaaatatg actttgttca agacaaccat tcactatcag ctgaagctgg
    11821 tgtccttcgg gggcttcatt tccaaaaagg agaagctgca cagacaaaat tgattcgggt
    11881 agtcactgga gcagttttag atgtagtagt ggatatccgc aaaggtagcc cgacttatgg
    11941 tcaatgggac ggatttatct tatctgaaca taatcatcga cagttgcttg tacctaaagg
    12001 atatgcccat ggattcgtta ctttgacacc aaatgtgaac ttcctttata agtgtgataa
    12061 ttactataat gcagaagcag atgggggcat cgcatttgat gatccagatt tagcgattga
    12121 ttggccaatt gatccatcaa gagcgattat gtcggaaaaa gatcaccgtc atccgacatt
    12181 aaaagaattt gaagcagaaa atccatttgt ttacggagaa atctaagaat taaggagaaa
    12241 ttttcttatg aaaaacatca ttgttaccgg aggtgccgga ttcatcggct ctaatttcgt
    12301 tcattacgtg gtaaacaatc atccagaagt gcatgtcact gttttggata aactgactta
    12361 tgcaggaaac aaagagaact tggctggact tcctagcgat cgcgtagaat tggtcgtagg
    12421 agatgttgca gatgcagaat tagtggaccg tctagtaaaa gaagcggacg cagttgttca
    12481 ttacgcggca gaatcccata acgataattc attaaaagat cctttcccat ttgtacaaac
    12541 gaatatcatt ggtacgtata cacttttaga agcttgccgc aagtatgatg tacgctatca
    12601 tcacgtatct actgacgagg tttacggcga tcttccatta cgtgaagacc tgccaggaca
    12661 tggggaaggc gaaggagaaa aattcactgc ggaaacacca tataatcctt caagtccata
    12721 ttcttctaca aaagctggtt cagatctgtt agtaaaagca tgggtgcgtt cttttggctt
    12781 acgtgcaacc atttcaaact gttcaaacaa ctatggtcct tatcagcata ttgaaaaatt
    12841 tattcctcgc caagtaacga atatcttaag cggtattcgt ccaaaattat atggcgaagg
    12901 aaagaatgtt cgtgactgga tccatacgaa tgatcattct tcagcagtgt ggctgatttt
    12961 gacgaaaggc agaattggcg aaacctatct gatcggtgca gacggtgaag aagataataa
    13021 gaccgtgatg gaattgatct tagaaatgat gggtcaacca aaagatgcgt acgaccatgt
    13081 aaacgaccgt gcaggacacg acttgcgtta tgcaatcgat tcgacaaaat tacgggaaga
    13141 gttaggatgg aagcctgagt ttacgaactt ccgtgatggg ttggctgaca cgatcaaatg
    13201 gtacgaagaa catgaagact ggtggaaaaa agaaaaagag gccgttgaag cagcatatgc
    13261 caaaaatgga caataataca gagaaaatgt cactgtatta atggaaaaac acatgtgagg
    13321 caggcgctcg cctgtctctt ttttaaaata aaaatcaagg agcggataat catatgtttt
    13381 tattaactgg aggaaatggg caactaggta cagaattgcg ccatttatta gatgaaaaaa
    13441 atctgcaata tgtttcaaca gatgcaaaag acttggatat cacagatgcc gagaaaacaa
    13501 tggcttacat cacagaatta aaacctgatg ttattttcca ttgtgcagct tacacagcag
    13561 tagataaagc agaagaagaa gcaaaagaat tagatgagaa gatcaatgtt gatggtacaa
    13621 gaaatgtagc acttgcagca aaagctgtga atgctcaatt cgtctatatc agtacagact
    13681 atgttttcga tggaaaaaac aaaaatgtag aatacgaagt agacgaccaa acgaatccac
    13741 taaatgaata tggtcgtacg aaattattag gcgaaaaagc agtagaagag attttagaag
    13801 actattatat catacgaact tcttgggttt tcgggatcta tggacacaat ttcgtcttca
    13861 cgatgcaacg cctagcggaa acccgcgaca aactgactgt agtaaatgac cagtttgggc
    13921 gaccaacttg gacacgtact ttggcagaat ttatggtata tgtcatcgaa gaaaaagcac
    13981 catttggtat ttatcatttg tcgaacgaaa atagctgttc ttggtatgaa ttcgcaaaag
    14041 aaatattgaa agatacagat gtagaagttg cacctgttac gtcagaagaa tttccgcaaa
    14101 aagcaacacg tccgcagtat tcagtcatga gcctgaaaaa gacagaagag ttaggatttg
    14161 tcataccaac ttggcaagaa gcacttgcgc aaatgctgga agctgccaaa aaaatgaata
    14221 agtaatttcc ttttatcaaa tagaatttgg agcatgatat ggaaaaacaa acaagaagtg
    14281 cacgacgaaa agaaaagcca aaagcgaacc aggcagattc gaatttggtg cttgacctgt
    14341 ttctaaaact ccgctatctt attggcgttt tagtcatcgt ggtcatcgtt acttttaatt
    14401 taaacggaag ttcacttggt gtttgggata aatatgtgtc gcaaagagac gatgggaaaa
    14461 aaactgatgt gatttttgga cagaatcggg aggtgcggtc agacgaatgg ctggtgcaga
    14521 caccttttta cctctcacaa gctgaagagg actttccgtt ggtgaacgag gattattcat
    14581 taaacggaca aaacatgatc attgcgtata attctcctgc aaaggatatt acggttatcg
    14641 gaaaaccatt caactgggga ttctttttcc tcggaagaga tcgaggactt tccttttatt
    14701 gggcgatgaa attagtcgtc atggtcttgc ttggattcga agtactgatg atattaacga
    14761 agagaaaaaa agctctatcg ctaatcggtg catttgcgct gacattttct ccagcagtcc
    14821 agtggtggtt catgcaacat gtgggcgatt tgattttctt tactttggga ctgatgactg
    14881 ctttttatca ttatttctac aatcatgata aaaaatggct acgaacgttg atgatgcttt
    14941 tggtggttat tttcggtatt gggtttattt tagtgattta cccagcccat caagtgatgc
    15001 tggcgtactt gctgattttt tattttgttg gcctgcttat ttactatagc aagaaagttt
    15061 catgggatct gttggatgta gtgttgattg gaggggcagt tttatttatt ggcggagtca
    15121 tggttcattt ctggctaacg tcaaaagacg cattgttagc ttctttgaat accttatatc
    15181 caggaaaacg ggtaagtact ggaggaaagt ggacgattgg cgaatttttt tctttcctta
    15241 caaattggaa aattcctttt gaagatatca actattcgaa taactctgaa gtggccctgt
    15301 tctatcactt ttttccaact gtattcctag caagtccatt tgtgttattt ggcaaaaaag
    15361 atagcgaaca aaagttactg ggacgtattt tgatgatttt ttgtctgttt tctatctttt
    15421 ggattaccgt tgggttgcca aaatggctag cagaaatcac tctgctgtct tatgttcctc
    15481 cattaagagc catccttact tttagttttg cggcttgttt attatcgatc tggtttatcg
    15541 cttatatttg gagagaagat gttattccta tttggtataa attcattttg ttggcagtaa
    15601 atgcatggtc ttatttctat gcgattacgc attcgaaaat agataattat ttttcggatt
    15661 ttgaaaccat cgcagttgct gtgttgggaa cagtgatttt agcatttgca ttatttggct
    15721 ggagacgctt gttttacctc gcttttgccg ccttagttat cgtatctggc tttttcgtca
    15781 atccagtcgt agaaggtaca ggagcgattt ttgaaaaaac actggcaaaa gaaatccaaa
    15841 aaatcgatca acaagatcct ggtaaagttt ggttgtctga agatgaatta tataacttca
    15901 ccccaacatt gggagtgaaa tccttcaata cagtacgctt ttatccagat atagctgcat
    15961 ggaaggaaat cgatccgaaa gaagaatacg agaagtatta taatcggtat gctcatacaa
    16021 gagcttttat cgctaaggaa gatacaaagt tcattttaga tcgtccagat atgttttcgg
    16081 tcacgttgaa ttttgaagac gcagaaaaat tagggatcga ctatgtgatc agtaaacgac
    16141 cattaactga ttacaaccaa ttatatgaag cgcagttcac gcagctatat ggaccggata
    16201 aagacggtta tatgattttt aaagtagaat atccggatta tccagatgta ccacaaacac
    16261 aaactcagga aaatcagcta gtcgtacagt aataggctag ctgagaataa tcctgaaata
    16321 ttagtagaaa ggagagtgga gataattcaa gcaatcgtac aaaaatttgt caacaaaatg
    16381 agtgtcggtt tcttgctttt gttgctgacg ctagtctttt ttgtttggac agcagggtta
    16441 ggttttccag caactttgtt tatatataac aatagttcat tacttatcgc ttttagtgtg
    16501 gctattttgc tcattttaaa tgtttataaa gcaaaaggcg tcgattttct ttatctaggt
    16561 gttgcgcttg cccttttcat tttcttttct tactttcggg aattaagaaa tgagtcttta
    16621 tttggagatt tactgtttcc tttgattatt ttattttgtt tagtaattaa atggatcgag
    16681 tggacaaaat gggatcgcgg tatttatctg attgtcttta cggtcttcct cggaatcacg
    16741 ttatatcgtg tatttacaga gatccaagta ccagaaggac aaagcatctg ggctccaggc
    16801 aattcgcttt ctgatatttg gatcaatacg aatacaatcg gaagttcttt gatgactctt
    16861 gctttattga tcagtggatt tgccagttct tttgaacgat ggtatgtacg aatcatcggt
    16921 gcagtagcag ttgttgcagc ttttctcggg atttgggtct gccaatctag aggtgcatta
    16981 gtcgcgttga ttgtttttgc gatattggat cttttaccta aatcgttgat gcgtgtcatt
    17041 cgtgcgccat ttattgctta cacggttacg attcttttag ctttgccgat ttcttatctg
    17101 gcggctgtct ctgaaaaagt caatttgttt accggtcgag aagatatttg gcataaattc
    17161 tatcagacgc taggagaaaa atctgaacaa gttttactag gaatgaaaac atttatcttc
    17221 caacgaggaa accaattttt aggaaaccac aatagttata attctatttt gaatatctat
    17281 ggattgatag gatttgggat tgctactttg ctgcttatcc tttttattgg tcgcttaacg
    17341 ttgaaagcag atttatccaa cgggcagatg acttttatct gggccttttt tgcggtgatg
    17401 atgcagtcat ttatggagga tacattgact tcatacccat gggttccgat tatttatatc
    17461 ctgttggcta tggccgttca tcgatatgat gaaccagaag ttgcggagga attctatgtt
    17521 atggaagagg catatgacga tatgacttat gatgatacat cttatgggga acaaccagat
    17581 agcgctcatt cttccaggat ccaacgctat cattgatagg cacaaaa
//
LOCUS       DAFEPQ010000054        17511 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.056, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000054
VERSION     DAFEPQ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.056"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            100..2427
                     /locus_tag="LP543_001940"
     CDS             100..2427
                     /locus_tag="LP543_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL2322104.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2553..2882
                     /locus_tag="LP543_001941"
     CDS             2553..2882
                     /locus_tag="LP543_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBL2322105.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2922..3926
                     /locus_tag="LP543_001942"
     CDS             2922..3926
                     /locus_tag="LP543_001942"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL2322106.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3923..5352
                     /locus_tag="LP543_001943"
                     /pseudo
     CDS             3923..5352
                     /locus_tag="LP543_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            5558..6730
                     /locus_tag="LP543_001944"
     CDS             5558..6730
                     /locus_tag="LP543_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL2322107.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6892..7347)
                     /locus_tag="LP543_001945"
     CDS             complement(6892..7347)
                     /locus_tag="LP543_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322108.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            7936..8358
                     /locus_tag="LP543_001946"
     CDS             7936..8358
                     /locus_tag="LP543_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2322109.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(8524..9717)
                     /locus_tag="LP543_001947"
     CDS             complement(8524..9717)
                     /locus_tag="LP543_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2322110.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            9941..10318
                     /locus_tag="LP543_001948"
     CDS             9941..10318
                     /locus_tag="LP543_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL2322111.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            10306..10644
                     /locus_tag="LP543_001949"
     CDS             10306..10644
                     /locus_tag="LP543_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL2322112.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            10778..11221
                     /locus_tag="LP543_001950"
     CDS             10778..11221
                     /locus_tag="LP543_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HBL2322113.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            11483..12298
                     /locus_tag="LP543_001951"
     CDS             11483..12298
                     /locus_tag="LP543_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2322114.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            12308..12970
                     /locus_tag="LP543_001952"
     CDS             12308..12970
                     /locus_tag="LP543_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBL2322115.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            13175..14833
                     /gene="alsS"
                     /locus_tag="LP543_001953"
     CDS             13175..14833
                     /gene="alsS"
                     /locus_tag="LP543_001953"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBL2322116.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIFMQPERELIGDISATLDLLTGSLEPQQV
                     SEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSHYI
                     WMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQDL
                     ETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGIRA
                     TSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            14846..15556
                     /gene="budA"
                     /locus_tag="LP543_001954"
     CDS             14846..15556
                     /gene="budA"
                     /locus_tag="LP543_001954"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBL2322117.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            15686..16183
                     /locus_tag="LP543_001955"
     CDS             15686..16183
                     /locus_tag="LP543_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBL2322118.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            16368..16628
                     /locus_tag="LP543_001956"
     CDS             16368..16628
                     /locus_tag="LP543_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322119.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(16990..17229)
                     /locus_tag="LP543_001957"
     CDS             complement(16990..17229)
                     /locus_tag="LP543_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322120.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT         5943 a         3042 c         3473 g         5053 t
ORIGIN      
        1 aaaaaagccc caaactttct atacatcaat aaaataataa aaattaaatt tagaataacg
       61 tgtaaaggat tatttgatga ttttttattg agaggaggga tgaaaatgat taagaaaact
      121 caaaataaaa tccaacgtat atgtcaaaaa cattttgttt ctagactcaa agctgtatct
      181 aaaagaaaat acttacatcc cattatgatt tcgatcatct tgatttttac tttcttaggg
      241 agcttatcaa gtatttatgt aaacgtttat acagaaacgt caaattttgc taaccaaacg
      301 acagaggaga caaatagtcc accctattct gaaatacagt ccatagaaag caccaatcag
      361 tctagcaaca acgagacaac ctcaaccaca tcctcgactc aactgattga tccgacagaa
      421 aaagaaatca cagcagcaaa agcagcaggg gagaaagctt attcagaaac aggtagaagc
      481 cagacattta gtactgttgc ggccactgcg aatttcaacg gacataccat accagatgga
      541 tatacagtga tatatgccaa agatggagtc attgttgcgg ttaacggatc gatgagtgcg
      601 gataaaactg cgttacaagg cgtaaccaaa gcaatcgctt ttattgataa ccgtccagat
      661 atccataatc tgtttattgg ctccacagta aatggtacta ctatcacatt cccaaaaagt
      721 atctatatgg gggtggctac cgctggaaat acgatcgatt cagttacacc caatgcggat
      781 aaaagttatt cttttacacc ctcaacatgg ggagggcttc ctgccggagg aggtatgagt
      841 actttttcaa tcggtataca gttagcaaat gatattccat tcacggggta ctcgatcaaa
      901 aatgggcaga tcgttgatgg taagggaaat gtttcaaatt ttccaaccta tcaagcagca
      961 acggttcagg tcaatgcgaa tcggacagga actttttctt atacagtcaa tggtggagca
     1021 gcaaaaatcg gaacgtacgg gactacatta tctggaatcc atgtgggtga caaagtggtt
     1081 atcactccaa cagcagtaac gggctattct tttgcacaaa caaacggttc tttcacagcc
     1141 ctagaaagta acgtcgatac ggtgacctat acaggcaaac aaacaccaat cactattaag
     1201 tatcaagact attttggaca gatgattgct ccttctaaaa taatgaatct tacttacgga
     1261 agtgctgcac aagacctgac gactaatacg cctactatca acggatatac ctttacagct
     1321 gtttcagcga cagaaacaaa gaaccaatca gcaatgagtg ttttagctag tttagataca
     1381 aatggaaatg taatcgtaaa ggacgcaaat ggaaaacaga tttctgaagt catcctatat
     1441 tataagacaa atgcaacagt atcaatcaat gcaaatggca gtaagtacta tgatggtctt
     1501 tctgttgtac cgatcgttaa atatgccttt aatgataaaa cagaatcaac atccctttta
     1561 aacaatgatc tcagtattga tcaaatcaca tggaatccaa ctgatttcaa agcgatgaat
     1621 gaaaagggag ttgaagtaac tgcgcctact gaaattggaa cctatacgtc ttggcaattg
     1681 acaaaaagcg gactagctaa actagctgcc agaacaaact atttatttac tatcgtgcag
     1741 acatctgatg tctatacgat taagcaaatc agcggtgcgg tagagctcag tggcagtaag
     1801 acatatgatg gaaaagcagg tgtcccgtca atccatgtca agctagcaga gggtgtcact
     1861 agttcgctta ctccagtaca agtggcactt agcagtatgg attacacagc agatgcacaa
     1921 tcagcaaaaa atatggttaa tgctggcagc tatacaatta aactaacgaa tagcggaata
     1981 gacaaagtca aagcagcaaa tcccaagttg tcatttactg atatagccaa cacatcagga
     2041 acttatacca tctataaagc taacgcagtg attactgtcg atgacgctgc ctttaattat
     2101 gatgcccaaa gtcactcgat ttcggttgga aatctccacg ttaccggtgt tgtcttaggc
     2161 gaatcattgg attataccct aacagttaac agccggacag atattggatc acaaatagta
     2221 acgattagtt taggcgaagg aagtgtaaac aataattata cgattatcgc aacagatacc
     2281 gctaagttga cgatcaatcc gactctcttg cttccttcga caggtgggat aggtctactg
     2341 ccctttgtct ttttaggact gatctttatt ttttctggct tcttttattt tatccatcgt
     2401 aagaaaaaag caggtgaaca gcgatgaaaa aaacaaaaaa tgtacgtgtg gatatatttg
     2461 ctgttctctt ttttatccta ctggtctttt ttataggttc tccgactgtg tcagctagag
     2521 aatcacctat tacatttact ttgcataatc gaatgttcga aagcgataag atgccaagta
     2581 tcagtcaaaa cgacggaata aatatcccgg ataactttca aggagtaaaa gggttgaacg
     2641 gcgttacttt cgctatatat gatgtctcgg atgaatttta caagctgcgg tcagaaggaa
     2701 gtagtgtgga agacgcacaa agaaaactag cacaaaaaag tgatagtgaa aaaatactgg
     2761 cagaaaatgt gacaaaaaca gtagacgagg aagagggcat tgcttctttt agtgcatcgg
     2821 acaaagatga gcaaggtcgt gacgctgttt accgatttgc ggaaataaag acttcagatt
     2881 aagtaaaaga aaaatcagca ccatttgtcg ttgtgcttcc tgtgacagat tcatctaatc
     2941 ataaattgac gaccatccac ttgtatccaa agagcgaaca aaaaatacct gctgcactca
     3001 cattaacaaa aacagtagag aataagcaga cagattttgc agatggggat aaagttcctt
     3061 atctcatcac cgtaacaatt cctgagaaca tcaacgagat aggaacctat acgatcaaag
     3121 atacagctga tcctcagtta tggctagaac cagaaacgat cgattttttg attggtggag
     3181 acaagattca tacatttcac acacaaaaaa caaaacatgg ttttgtctta tcagattctg
     3241 gaaaagatct tagttcgttt gctgggaaaa aattaacgat ccgttaccaa atgacattga
     3301 aagaaaacag tgaaaaaacg acatttgata acgatgtacg tttgacgact gacaatcaaa
     3361 cagtagaaac acatcttgcc atccaaacag gaggcaaaca atttgtcaaa gtggatcggc
     3421 aaacgaaaca aaaactagca gatgctcaat ttctagtcaa aaaaaatgac caatacttag
     3481 cgcaagaaaa cgggataaat cattgggtag ccaaagatga ttcaagagca acgattttta
     3541 cttccagtaa agatggaaca ttttctgtcc atggattgtc ttttggaagt tacgaactag
     3601 tggagattaa accgcctaaa ggatatatat tgagtcaatc aaccattctc tttgaagttg
     3661 aaaaaggcag ttatgatccg cttcatccta taccattgga aatcatcaac gaaccaaaag
     3721 caaatccgac gaataatgga aaaacgccag ttcagatgca gacaaatggt ccatcaaatg
     3781 aaaaaaatgg aggaagcttt ccaaaaacaa gcgaagagat gcttggaggt ttctcaattt
     3841 tagggttgtt gctagtcctt tcaacaggga cagcttggtt ttataaaaaa caacaaaaag
     3901 gaaacaatag gagggaaata aaatgaaaaa catcaaaaca aaaatatgga cgtttttagg
     3961 aactgctatc atgctgcttc ctttcgttct cggcctagga actgcagagg tttctaggcc
     4021 gtatcaccta caccagaaaa tgttacggtt aatttgcata aattgaaatt cacttcagca
     4081 ccagaaaatc aaataaacaa cggaacggaa ttgacatttc cgaatagcga accgttgaac
     4141 ggcgttgaat tcaacgtata tgacatcact gctacgtact atccaagtaa agacacagca
     4201 gttccagcag acgctacacc cttcgcaagt gtaacgactt ctggtgaagg attagctaac
     4261 ctgactcttc caggtaaatc tgatgggaaa gatgctgtat acgtctttgt agaaacacca
     4321 aagcccggtg tcgaaacgtc gccaaatatc gtgttaagct taccctttta taacccgaat
     4381 tcatcagacc ctgacaaacc tttagacacg attcacctat atgctaaaaa tgtcatcaaa
     4441 gaaagcaata tcaaaatcca gaaagctggg aacttttcag gggtagataa gttagcaggg
     4501 gcgcaattcc agatcaaaaa ttcagagaat caattcgtga ccggttttga tgaaaacact
     4561 ggtgtcgctc tctatgatcc tgctgagtca gcgttgacat tcacaactga tactgaagga
     4621 tccatcagtg tcaatggatt aatagacggc aattatactt tagttgaaac aaaagcacca
     4681 gatggttatc aattaaccaa tccacaaaaa gaaactactt ttatagtaaa aaacggaaaa
     4741 ttgcaacaaa caaccggtat catccatggc gcagatgaag ccaatcttgg gaatccaaac
     4801 ggtgtagtca atcgggaaaa tacaactacg gataacacga ttactgtaga aaatgtccaa
     4861 aactacggtg atttccactt catcaaacaa gatgtgaaca cgcaagaaaa attagccgat
     4921 gcagaatttt ctattctaag agcaaaagat ccaactgtta aattaatgaa aaaaatagca
     4981 aatcttgcct caggagaata cccatatgct tggtcagatg aagtcacagg ttctgactgg
     5041 gaagaagtga aattgatttc tgattcagaa gggaaatttt caatcgaagg gctgaaacaa
     5101 ggggcatact gccttcaaga aacaaaagca ccagaaggct acgcattacc taagaatgaa
     5161 gctgcgtaca ctgaatttac agtagatgat gatccaacaa cttctgatga aacaacgatc
     5221 gataaccaac ctaaaggaat cttgccttca actggtggta tggggatcat cgtattcatc
     5281 ttggtaggta ctgctttagt tggaggagca gtgatctact tcaaaaaacg ccatcaagaa
     5341 acagaagctt aaactattgg aaacaaaaat catacgataa ataaacgata gtgattcaaa
     5401 tggggagaaa ggttgtgaca agtggtcggt ctcttggtat tcgctcaaca aacggcaaaa
     5461 taggaggaac tattttgccg tttgttgagc gaatactcag gtcaaggcgc tgatttcaca
     5521 acctcttcat atacatatta gaaaataggt ggacatgatg aataaaagca gaataagaat
     5581 actggacatc ttcatggcaa tcattttagt cgttgggatc gggatctttt cttatccttt
     5641 tgtagaagat tcactgaatg attttcttgc ccagcaaatg atcatccatt atcaaaagca
     5701 agcatcgaat aagaattcag aggaaataaa aaaacagcag gaaaaaatga caaagaaaaa
     5761 ccaacagttg gcagagcaaa acgtatcacc agggataggc agtttcaacc aagcagttga
     5821 tgcaaaagca ttaaaagatt tacctagtaa tgcctttttt atggaacata tgcttggagt
     5881 catcgaaatc ccaaaaatca atgtcagctt acctatattc gatcagacaa cggaaatatt
     5941 tttgcaaaaa ggaacgtcat tattggaaga aagttcttat ccgactggcg ggaaaaacac
     6001 ccatgccgtt ctttctgggc atcgtggact cccagaagca aaattattta cagatttgcc
     6061 taagttgaaa aaaggagacc agtttttcat ccagatcaat ggaaagacat tggcgtatca
     6121 agtagaaaaa atccaagtgg tcttacctga tgaagtagat tctttgggta tccaaaaagg
     6181 cagagatctc gtgactttgc ttacctgtac tccttatatg gtcaatacac atcgactgct
     6241 tgtgacgggg catcgtattc cttatcaagc aaaagaggca aaaaaagcga tccaaggaat
     6301 tgatcaatgg aaaaaatgga aattttttat ttggtttatc ggtatactac ttggatcaat
     6361 cggcttagtt tggctactca tcgcttattt agattctttg gccatcgcta agcgaaacta
     6421 tcctttatct ttttatgtaa agaacacaaa cggacgacca atcgaaggaa tggtcttttc
     6481 tgtaaaaacg ctcaacggca aacactatat tactagagaa aaagttccat ttgtgaaagc
     6541 ttctgatgaa tacggactag tgagattttc tgatttaaaa ggcaggaact atcgcttgca
     6601 acatgaggag ctcctgctta aaatccatgt aaaacataaa cattcgaaac agttttcaat
     6661 gaaattgaaa aaaggaagat ataaactgcg aaaagaaaaa gaggtgtact atttgattga
     6721 gaaagaatga atgaagatgt ccaaaaaagc agagggaaca gactaaatga aaaaagagag
     6781 aagctgacga aatggacgtg taaactgcta tttttggttt actatctgga acgccagctt
     6841 ctattttttt atgtttcaga agaattttat tattatagta taacttttga attagtttcc
     6901 ataacatttg gagcaagggg ttaaaccacg agcttgagct tcgcttaaag tggcaggggt
     6961 atacgtacca tttccacatt tatgtgtatg gtattttgaa ccggtcggtg tgacatagac
     7021 agtttgttca ttattttgct caggagctgc ggcttgtgcc tgctgagagg cctgttcagc
     7081 tgctgcttgc tgtcgcgcct gttctgcttg ttgctgtgcg attgcttctt gttgcgcttg
     7141 ttctgcttgt tgtcgtgcaa tttcttcttg ctttttccgt tcttcttctg cttcttttgc
     7201 tttgattgtg gaatcaattg cagcaagacg gcttgtcaac tcttgtttgt tttcaggtag
     7261 cgcttggata gctgctgaag cagttgcata gttttctttt gtaggatttg cttcagcttg
     7321 tgacaaagca gattccgctt ctgtcacttt tttttctagt gcagcttttt ccgctttttg
     7381 tttctcttct tcttttttct tttcaatttg ttcttgttct ttcgatgaat ctgccgtact
     7441 ttcggagatc gtggtactgc tgctggaagc ctgagctgaa cttgtgcttt gagtagtaga
     7501 cgtagtagca ccacacccac tcaacaaact tcctgcgacc aaaaacaatg caactaattt
     7561 tttcactaaa tttcctcctt aagtaaaaaa caaccgtttt caataaatgt ccttttcaat
     7621 tgtttactta cttgttataa taattgtgtt atgaatagtg attgaggaaa cttaatctaa
     7681 tcccgttttg atacacggga ttttttgcta tagttctgtt attaagtatg caataataaa
     7741 atatgtacgt tattttatta ttagattatt gacatttata taataaacga ttaaatgatt
     7801 catatcaata gaatacttag gaaaaaaaga gaaataataa caggtttatg cactattgac
     7861 ggtgtgacaa tatgatattt gtatccgttt ttttcttgta tgtagtatag taaagtagat
     7921 acaggaggag aaaatatgca gactttttta acaaaagttt ctcaaaagaa aattaaaatg
     7981 attcatttac tgcttgaaac agaacgttgg tgttcaatag aagaactaca aaacgaattg
     8041 aaggtatcaa gtaagtctat cttgaattat ctaacagatt tagaagaatt gttccaaaaa
     8101 tatcctgata aggtcatttt gaagaatgag aataatcggc gcttttctat ggaaaagcag
     8161 gagaacttcc caatctacat tatttatctt catttctaca gaaaatcata taattatcat
     8221 ttgattgaat tcatgtatca acatcctgag aaaaatttgg aagattatgc agacgctcaa
     8281 tatacgagtg tatccacagt ttttcgttat gctaagttat tagtaaagta ctttgaaatg
     8341 acggacgtca aagaatagta gccgccagat agaaggaaat tagcagttga aaaagctgcc
     8401 ctgcatcaag taatacgacc agccaaacgg aaacagtgtc tcatgttttt gatttggctg
     8461 gtcttattac cgcagatgca agcttttgcc acacctcttt cttgtcattt acagactcaa
     8521 ctatcaatgc tgcaagcttt cccttaatgc tgtaacacca gtaagagttg tcaagaccag
     8581 cagactgact aggtaaatac cgctcaaaaa caagattacg gcagttaaac tataatgatc
     8641 catcaagaaa ccgatgacga tagaagaaaa accaccaaca gctcgtccaa ggttcataat
     8701 gaaattattc gccgtcgcgc ggatttctgt tggatacaaa ctactaatca ctgcgccata
     8761 tccaccatac atgccattga taaagtagcc gactatcaca gcggctgcta ccaaactcca
     8821 ctggctatgg gctaacacaa gtagatagac agataaggat gaacaaatta aaaacaaaga
     8881 aaaggctagt ttaggcccca atttatccat gattatgcca aaagtcatca tccccaaaga
     8941 catgcctaaa atagtactga ccatccacaa tgaagaccca gaaaccgaaa gccctaatcg
     9001 attttgcata attgaaggca gccaattcat taacccaaaa tagccggcaa tttggacagt
     9061 taccatgata ctaaggccac atgtttgcca agcaagatgt tttgattcaa acagtctgct
     9121 aagtttggtt ttcttttcgg cagattgtac agatttgaat tcctttggtt cagtcaagcc
     9181 gcgacgaatg aacaagacca tcaacaccgg cagtacacct acgacataca acatcttcca
     9241 accaaacgtt ggaatgatca cagcagctaa aatcgcagcc aaagccgcac cgacctgcgc
     9301 accaatacca gcaaccgaag aagctcgtcc aatttgtttt tttgaaaaac tttcggaaat
     9361 caaggacata cacgccccgt attctcctcc cgctcctaca cctgcgataa agcgaaagag
     9421 ataaaccaag tagatgtttg aagcaaaata catcaataaa gaagcaatcg aaaacagaag
     9481 gaccgtttga gaaaatactt tcacacgtcc atatttatca gctatgatgc caaaaaaaat
     9541 acctccaatg agcatcccca aattagtgat cgttgaaatg aaacccgctt gcgtaccact
     9601 aatatgaaaa gaagtaatca ttgaagataa tgaaaaagct aaaaacataa tatccatatt
     9661 ctctaaagca attcccgccg atgtcgataa taataacttt cgctgatacg ttgtcatttt
     9721 tatacccctt ttcctaaaat ttccctaaat tgttaaaagt atatccaaaa aagaataaaa
     9781 aaacaagact tttttatttg aaataataca caaacaaccg atatagataa tccagcttag
     9841 ttttaataga aatgagtatt gacttggagt acacttaaag ttgtagaata aataaaatac
     9901 ataagtaaag tggcaagaaa ttaagagaaa gagtgatcag atgaatatca aacaagtcag
     9961 tgaagaaaag ggcatttcgg ccgatacatt gcgctattat gaacgaatcg ggttgattcc
    10021 accagttaat cggacaaaag gcggaatccg tgattataca gaagaagacc taaaatgggt
    10081 cgactttacg atctgtatgc gaaatgccgg attatcgatc gagtctttga ttgaatatat
    10141 tcgcctatct agcgaaggag aagcaactgt atttgagcgg cgtaagcttt tgattgaaga
    10201 aagtgaactt ttgacgaaaa aaattgctga aatgcaagaa tgtttggaac gtttacaagg
    10261 aaaaatcaag aaatatgatc gtgtgttatc tagaaataaa atggaatgta tcaagtaaaa
    10321 aaatcaagga gcatcgctat ggagttacgc ttactaaaat atttttggac aattgcagaa
    10381 gaaggaacaa tctctcaagc cgcagaagtt ctacatctta cacaaccaac cttaagccga
    10441 caactgaagg agttagaaga ggaactagga acagaactgt ttgtacgcga aaaccgcaaa
    10501 atgatcttga cagaagcagg ttattttcta aaaaagccga gcagaagaaa ttttagattt
    10561 aacgaatcaa accactcaag aattcgagaa cagaaaaaaa caattattca gcggccgatt
    10621 tacgatcggc tgtgtggaag ctgataattc gaatacattg gcactattac tagaaaagtt
    10681 gatccgtgat tatcccagcg ttcaatttca tatccacagc ggaacaagtg atgatatcac
    10741 ggagtgacta gaaaaaagcc tgttagattt agcgattttg ctagatccag tagatacaga
    10801 gaaatacgaa acgattttgt taccaagaaa ggaaaaatgg ggattgctca ttttagaaaa
    10861 ttctgtttta gcaaaaaaag acgtgataac aaaagaagac tttgcgaatg tacctctttt
    10921 gatctcaaag cgtccagaaa tacagcgaat gtttgctgac tggttaggca taaaaaagga
    10981 gcagttgctg atcacaggaa cgtacaattt gattttcaat gtatttgcgc taattgagaa
    11041 tcaggtaggt gctgctttag tcatagaagg tgctgtaagt catctttcaa agcgagtgac
    11101 attcgttccg cttgacccaa tgattgaaac gaactgcatg ctagtctgga aaaagaatcg
    11161 gattttatca cctgtagtca atgaattgat tcaccgtttc aaagaagctt ttgaacagta
    11221 ataagtatct ctaatcgaag aaatttcaat acaaaagcga gacgtatgac agtcttgctt
    11281 ttgtattttt tcatttttaa atcaaaaaaa ataaagagat ttacataact tgttaaataa
    11341 gcgcttacaa acataagaag aacggaaatg aatcagctat aataaccgtt gttctggatt
    11401 gtaatcatta caaagaaatg aagattataa ctttggaggt gttcgaaatg aaagaaaaaa
    11461 agcaaaaatt cgttcaaaaa acatgccctt tattcaatgg ttgggtatga ttctgctgat
    11521 tgtattagct ttattgacga tcattccttt tttatggatg gtttctgctt cattcaaagc
    11581 aaataatgaa gtgttcacga ttccgattca atggatccct aaaacttggc atttagagaa
    11641 ttatctggtt atttggtctc gtattccttt agtgacattt tttaagaata cagctttttt
    11701 aagcgttgtg atcacgttga ttcagttgtt tacttctagt tttgctgctt acgggttttc
    11761 taaactaaat tttaaagggc gcgatatttt atttttagca tatatcggca cgattgctgt
    11821 cccttggcag tcttatatga tcccgcagtt cattatgatg agaaaagtgg gtttaacata
    11881 tacgttgtgg tcgttggtgc tattacaagc gttcaatgct tttggtgtgt tcttgttgaa
    11941 gcaatactac agtagtatcc cagattcttt atgtgagagt gcgcggatcg atggcttatc
    12001 agagtgggga atctatcgca agatcatttt acctcttaca aaaccggcga ttgccagttt
    12061 gacgatcatc acattcgtta atacatggaa cgattatatg gggccattca tttatttgtc
    12121 ttctacggaa aataaaacaa tccagttagg gttgaaaatg tttattgggc tatttgatgc
    12181 agagtacgca ttaatcatgg ctgcctcagt tgtttctgtt cttcctgttg cttttatatt
    12241 tttagcgatg caaaaatatt ttgtagaagg gatcgcttca tcaggaatga aggaatagga
    12301 ggtgattatg aaagagcagc gttttgacaa aaatgtttat atggaattct tttattggtt
    12361 gtatcaatca ttggcaagta gcctttgttt ttggtgtgtc aatatcttgt atatagcagc
    12421 tgcgttcctt ttgtcgatag atggaaggaa tgtcttttcc tttatttttt ctttactttt
    12481 tattggtcca ggttgcatcg tgatgatagg tatgacagaa gaattagcaa aagaaaaaag
    12541 tttttcacca gtacgtttgt tcataaagac gtttcgccaa ttttggctaa agggattttt
    12601 ttattggcta tttgcttgga tagttagtgt gataatgatt tttgattgtt tcttttttat
    12661 acgtttttct tatggaaaat ggttgattcc gttgtttgtt ttacttgctt gtttatcagt
    12721 ttcctttagc atcaactact ggtattttca ggtgagaaat ccagcgtcga aaccaaatca
    12781 agttctacgg atagcgttct actatacgtt gaaaaaatgg tatgtgagtt tactagattt
    12841 tttactattg acaagtttgt ttctcttctt tttcgtaaag ccccaatggt gtattctact
    12901 aggtccttct atcgtatttg gtttgattta cttcaacaat cgtaaattga tgagaactat
    12961 ggatctgtag actttataaa ttgaaacttt caattaagaa tgacagtttt tatgtaaaaa
    13021 ctgtcattct ttttcatttt ttgaaacata tggctaaaca aatgaaataa aacgtgctat
    13081 acttttgaat gaaacaggtt tagaaactgt atcaattatt tgtgattatt tctaactaat
    13141 aattaaaaat aaagcataaa aaggacgatg ttcaatggac gaaaaaatga aacaaggttc
    13201 gaaatctgta gtagaaagtt tgattaatca tcatgttgat tatgtattcg gtataccagg
    13261 tgcaaaaatc gatggtgtat ttaatgaatt agaggatcaa ggtcctgaat taatcgtgac
    13321 tcgtcacgaa caaaatgctg catttatggc acaagcaatc ggacgtatta caggagagcc
    13381 aggagtagtt atcgcaacaa gtggtcctgg tgctagtaac ttagctacag gtttggtaac
    13441 agcgacagca gaaggtgacc cagttttagc aattgccgga caagtaaaac gaagcgatct
    13501 attgaagttg acccatcaaa gcatggataa tgcagcactg ttccagccaa tcacaaaata
    13561 cagtgcagaa atccaagatc cagaaacaat ttcagaagtg attgccaatg cttatcgtat
    13621 ggctaagagt tcaaaaaaag gcgcgagctt tatcagtatc ccgcaagatg tagtggatgc
    13681 acctgtccaa gggaatgtca tcaagccatt gagcgatcct aaattaggtt ctgcttctgc
    13741 agatgatatc cgttacctag ctgaacgcat tagagaagca aaactaccgg tactattagt
    13801 aggtatgcga ggatcaagtg aaaaagaaac gttagccatc aggcaattag ttggaaagac
    13861 agcattgcct gttgtagaga cattccaagc agctggagtg atctcacgtg aattagaagc
    13921 gcatttcttt ggtcgtgtgg gtttatttag aaaccaacca ggagatatgc ttttgaaacg
    13981 aagtgatcta gttattgcta ttggctatga tccaatcgaa tatgaagcaa gaaactggaa
    14041 tgcggaaaaa gatgcgcgga tcattgtgat cgatgaagcg ccagctgaaa tttttatgca
    14101 gcctgaaaga gaattgatcg gtgatatctc agccactctt gatttattga caggttcatt
    14161 agagcctcaa caagtgtcag aagatgcaaa agaatattta gcatctcttc aagcagaatt
    14221 aacggagcgg gacattgttc aatcaaaagg agaagcaggt atcttgcatc ctttagaagt
    14281 tattaacacg cttcagtcaa aagtaacaga tgatatgact gttaccgtag atgtaggtag
    14341 tcattatatt tggatggcac gccactttag aagttatgaa cctcgtcatt tgttattcag
    14401 taatggtatg caaacgcttg gtgttgcatt gccatgggcg atttctgctg cattggtgcg
    14461 tccaaataca caaatcgtat cagtttccgg cgatggggga tttttattct ctgcccagga
    14521 tttagaaacg gcggtacgta aaaaattgaa tatcgttcat ttgatttgga atgatggaca
    14581 ctacaatatg gtagagttcc aagaaaaaat gaaatatcag cgggcgtcag gtgtagactt
    14641 tggtccagtt gattttgtga aatatgcaga agcttttggc gctaaaggaa tccgagctac
    14701 aagtgtagag gaattagaaa aagctttaga agaaggattt gcgacagaag gacccgtaat
    14761 tattgatatt ccgattgatt accgtgataa tgagaaattg ggagaaacaa tcttaccaga
    14821 tcaattctat taaggaagtg tcgacatgac tgaaaaaatc ttataccagc acgggacatt
    14881 aggagctttg atggctggct tgatggatgg aacagaaacg attgctcata tattagaaaa
    14941 aggaactctg ggattgggaa cattgcatgg gcttgatggt gaagtcatct ttttagatgg
    15001 cgtagcttat caaggaagat cagatggctc agttgcccaa ttagatggaa gtgaacttac
    15061 cccctatgca gcaattacag attttacgcc agatactagt ttttctgtct caaaaactgc
    15121 agatggagaa caattgaaaa aagacatatt agtaagagaa gccggggaaa acctattttt
    15181 ggcagtgaag ataactggat tattcaaaaa tatgcacatc cgtatcatgc cgaagcaaga
    15241 aaaaccttac agaagactag caaaaatttc agagtctcaa ccagaattcc aacaaagcaa
    15301 tgtccaagga acattagtcg ggttctttac gcctgaatta ttccaaggag ttgctgcagc
    15361 gggatttcac ttgcatttta tcgatgatac acatacattt ggcggacatg taatggattt
    15421 tgaagtggct gaaggaaaag ttgagattag ccgcatctcg tcccttgtac agcattttcc
    15481 agttgatgat ccgacttttg ttcacgaaac gattgattat gctgatttag cagcagaaat
    15541 ccaacaagca gaatagaaaa agacagcgag atgcgttcaa ggtgattaac ctgacgtatc
    15601 tcgttttttt gaggaggaaa cctagcgttt acaaaaaaac tgagctataa tcttctccat
    15661 agatcaaaaa ggagcggttt ttatcatgtg gacagaaaaa cagaaaaaag atgcatacga
    15721 aattttatta ttacagcaac aaggattatt agaagcagaa gataactggt tagcaaattt
    15781 agcaaattca tctgcattgt tgaatgagac tttgcctgag actgtctttg caggatatta
    15841 tttattcgat ggtaatgaat tgattttagg accgttccaa gggcgagttt cttgcacacg
    15901 tatcaagatg ggaaaaggag tttgtggaga atcagctgaa aaacaagaaa cattgatcgt
    15961 tgatgatgtc aaaacccatg caaattacat ttcctgcgat tcagccgcac aatcagaaat
    16021 cgtagtgcca atggtaaaag aaggtaattt aatcggtgtt ttagatatcg attgcggagt
    16081 aacgaaaggg tatgatgaaa tcgatcaagc atttctagaa aaatatgtag atcttttatt
    16141 agcaaatttt aatataaatg ctttttcatt tattaataac taaaatcgtc tattgaaaca
    16201 aaaaagaaga gaaatcaaga ataaaaagag ccggaaagac agtgggcatt tctgtttttc
    16261 ggttttttta tttttttata aaatcacaag tgttattgat tttcataatc atattttaat
    16321 ggtattatca ttttaaataa tggaaaaaca ttaaggatgg atgaaatatg aataacgaaa
    16381 ctatcatttt aggaaaaaaa tatatttgcc aacctattgg cttgaaacaa aaagtggttg
    16441 gagaagtaat cagtaaatta gaaaattgtg ttgtgatctg cgtagacgaa tatcatgacg
    16501 tcgatcgcaa tgaaatatta gaaaaatgcg gaaaagtagt ggcaaaatat actcacgttt
    16561 atgagttgga aacaacagaa tatctatata actatagcaa caaatatgaa catactttag
    16621 tttcataaag atttaaataa agtgccttca aaagcgagaa gaccaagctt ttgaaggcac
    16681 tttatttagt tggcagtgat tgttcatgat ttttgattta ttcaaaaggt tcgcctagag
    16741 aatctgtctc tgtaacgagg cctggtggag aaactcgata aaaacctata ctgccagaac
    16801 tgtcctattt tcggatagac aaactagtag ttttaaaaag atcatccgat ccaatcattt
    16861 caaaaaatgc aaaactgata tcaggatcat ttagctcagg cttgttatcg taaagcattt
    16921 gtaaaggttg catttagtta ggagtaaacg cgaaactatc atcattattt gctgaagaag
    16981 atgaagaagc taattcagag tttgtttgaa ataaaggaga cgtgctgaca gtttctttac
    17041 cctgtttttt gcagaggtag aaacttttat atcggaagtt tctaccggag aatttttctc
    17101 atttgtagaa attgttgaat cgatactatt cgaaatttgc gtatctgaag gtatgttctt
    17161 tgatgcagaa gtcccacaag cagtaagtaa caataaattt gataaactga taaacagaat
    17221 tcttttcatt atgtacacac tccttatagt atcgtttagc atacacctat gctcagtatt
    17281 aggcaaaatc aatgcaaaac tatcaagtct tttatagtat ctattagtct tagtaataca
    17341 tatatggaga gggtgatatc ccagttcaaa ccagatacat tggttaccat ctgtattaac
    17401 gattatgcct ctaaacgctt tttttatgaa aaaatttata agtgaaaacg taggtatgat
    17461 attatctaga ggtaattttt ttgggtattt aaatagttgc taggaggata g
//
LOCUS       DAFEPQ010000055        16591 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.057, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000055
VERSION     DAFEPQ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.057"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(45..779)
                     /locus_tag="LP543_001958"
     CDS             complement(45..779)
                     /locus_tag="LP543_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBL2322121.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRFVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1105..1515
                     /locus_tag="LP543_001959"
     CDS             1105..1515
                     /locus_tag="LP543_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBL2322122.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     EGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            1508..2209
                     /locus_tag="LP543_001960"
     CDS             1508..2209
                     /locus_tag="LP543_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBL2322123.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2209..3822
                     /locus_tag="LP543_001961"
     CDS             2209..3822
                     /locus_tag="LP543_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2322124.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            3812..4033
                     /locus_tag="LP543_001962"
     CDS             3812..4033
                     /locus_tag="LP543_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBL2322125.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4030..4791
                     /locus_tag="LP543_001963"
     CDS             4030..4791
                     /locus_tag="LP543_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBL2322126.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPETFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTSGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRRKSGTIINMASIAGLVAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5172..5681
                     /locus_tag="LP543_001964"
     CDS             5172..5681
                     /locus_tag="LP543_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBL2322127.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANMASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5751..6290)
                     /locus_tag="LP543_001965"
     CDS             complement(5751..6290)
                     /locus_tag="LP543_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBL2322128.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVVVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6430..7041)
                     /gene="rpsD"
                     /locus_tag="LP543_001966"
     CDS             complement(6430..7041)
                     /gene="rpsD"
                     /locus_tag="LP543_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBL2322129.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7303..8154
                     /locus_tag="LP543_001967"
     CDS             7303..8154
                     /locus_tag="LP543_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBL2322130.1"
                     /translation="MRGHVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPAATILVEGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLAYFASQGQTDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8182..8817
                     /locus_tag="LP543_001968"
     CDS             8182..8817
                     /locus_tag="LP543_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBL2322131.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVYYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8837..9610
                     /locus_tag="LP543_001969"
     CDS             8837..9610
                     /locus_tag="LP543_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL2322132.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10018..10815
                     /locus_tag="LP543_001970"
     CDS             10018..10815
                     /locus_tag="LP543_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBL2322133.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKKIQLIQINDDRPFME"
     gene            10793..11206
                     /locus_tag="LP543_001971"
     CDS             10793..11206
                     /locus_tag="LP543_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL2322134.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11190..13835
                     /gene="mutS"
                     /locus_tag="LP543_001972"
     CDS             11190..13835
                     /gene="mutS"
                     /locus_tag="LP543_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBL2322135.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLKKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKNSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13853..15961
                     /gene="mutL"
                     /locus_tag="LP543_001973"
     CDS             13853..15961
                     /gene="mutL"
                     /locus_tag="LP543_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBL2322136.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPVV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15983..16543
                     /gene="maf"
                     /locus_tag="LP543_001974"
     CDS             15983..16543
                     /gene="maf"
                     /locus_tag="LP543_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBL2322137.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARIVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
BASE COUNT         5387 a         2964 c         3598 g         4642 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaaaacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc gagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctaa ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 ttcacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaagaaac atcaacacga aaagcatcgg
      841 tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata aaaaaatgaa
      901 ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt aaaagttctc
      961 cacaaaccac caagcttaac tataaatata ccaaaaaaaa aacgtttttt ttctcaaaaa
     1021 ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag taaaatgaaa
     1081 gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat tattattttc
     1141 ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc tggaagtatc
     1201 ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc tgaaaaaatc
     1261 ggagcaactg gtgatttttt attgaataat ctagctattt tatttgttcc tgctggtgta
     1321 ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact gggagcagtc
     1381 gtcgtgtgtt ccatcgtcac gatggttgca gagggaaaaa cggcagaagg tgtagaagct
     1441 ttgctgggct atgtccgtaa gaaaaaagca ggaagggtat ctgtaaacga aatggaggaa
     1501 aatcaaattg actgagtttt ttgtgaatcc actatttggc ttaagtttga ctttaggagt
     1561 gtatgtatta tctgataaat ttttgcggaa atataaactg cctgttttga atcctctcgt
     1621 attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct ataaagacta
     1681 taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg cacttgccat
     1741 ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa tggctgcgat
     1801 tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact tcttctctct
     1861 tcataaagag atggtgatct cattactacc taaatctgta acgacggcta tttctgtcga
     1921 tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg tctctacagg
     1981 tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg aaagtccagt
     2041 tgctcgcggt gtggcgttag gaagtacttc tcatgctatc ggtacagcaa aagcaatcga
     2101 gattggcgaa atagaaggaa tcatttccgg acttgcgata tgcgtaaatg ggatattgac
     2161 cgttttgttg ctgccgttat tctttcagcc ttttgctggc ttgttttgat gcgaaaacca
     2221 ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa agacgtgtat
     2281 ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat tctcctccta
     2341 gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt tgttcagctg
     2401 gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca gcagtttagc
     2461 ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt ttatgatacg
     2521 ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac gctgaaattc
     2581 cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt tgcgcaaaag
     2641 cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca gcaactgcaa
     2701 acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga agaaatcatt
     2761 cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg gccatttgaa
     2821 acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt ttttaatgtt
     2881 cgtctgaacg agatcaaaaa aagacgattg gaatacatgc taggtgcctt ttttttaaga
     2941 aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat ttctgacaat
     3001 ctgttgttcc agcgattttg ccaagtcatg gagccggtat ttcctaatta ttttcaagta
     3061 gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata ttatagtgat
     3121 cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc gccctttaca
     3181 gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg gctgccagca
     3241 gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt cgcctatctt
     3301 tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa tcatttagtt
     3361 attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag acacaaacat
     3421 cccaatcatc tggcatttaa aaatcatgcg tttttgatgg gtcggtatct gactgtgttt
     3481 aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt aatgacagat
     3541 ttcccattat ttgaagagca actgttagag gagggattac ggacattttt ccgtaatgag
     3601 tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct tctgatttcg
     3661 acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt gaccgaagaa
     3721 ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca aaaaaaaact
     3781 attaaaacaa taacaaagaa ggagaaaaaa gatgacattt gaagaaattt taccagaatt
     3841 gaaaaaaggt gcaaagatta ttcgtaaagg atggagtgga tttgaacttt atgttgttct
     3901 cgtatccgat gatgaatttg atggctgtcc agtgacacct tacttattga tcaaaacaag
     3961 tgacgaaggt ttttccagct tcgctcctac agtttgtgat attttagcag atgattgggc
     4021 gatcgtagaa tgaaaaacca gtatccagat ctagctaaac aggttgtatt tatcacagga
     4081 gctgcttctg gaattggtgc tgcgcaagcc cgagcgtttc ttgaacaaga tgcttttgta
     4141 tttggattag atcaaaagtt cggtaaaatg gaagaactct ctttgcgatt tcctgaaact
     4201 tttgcctacg cactaggtga tgttcgacaa atgaaagaac tccaacaagc ggtatcgaaa
     4261 tgccaggatc gttttggtga agttacgatt ttactgaata catcggggat attggatgcc
     4321 tacaaaccat tgatggaaac tgacgaatcg ctgtgggatc ttatttatga gacgaatgtg
     4381 aaaagcatgt atcagctgac aaaaatcatc cttcctgata tgatccgccg aaaaagcgga
     4441 acaatcatca acatggcttc gattgcgggt ttggttgctg gcggaggtgg gatcgcttat
     4501 acaagtgcga aacatgcaat cgtcggattt accaaacaac tcgctctgga ttacgcagga
     4561 cagggtatcc gagtaaaagg aattgctcca ggagccatcc aaacaccaat gaatgcggct
     4621 gattttgaag ggaaaggtga gatggctgag tgggtcgcaa atgaaacacc agtcaagcga
     4681 tgggcacagc cggaagaagt agctgagctg acgttatttc ttgcctctcc tcaagcaagc
     4741 tacatacaag gaacgatcat cccaatcgat ggcggatggt tgttaaaata accttgtgtt
     4801 tttttcaagc ttctgtatga taaaactaaa gagagaaaca acggttgatg ataaaagaaa
     4861 gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt ctttttaata
     4921 atcgttaata ttcgaaacta agcggctttt cacgaccttt tgatataaac ggtgttcaaa
     4981 agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct atttttattt
     5041 gactattctt attacttgtt gcagggcagc tttttcacta ccttctgcgg cacttcagtt
     5101 ggaattttct tcccatcctt tggtcgcagt cattaccaag ataaaattat cttgcaaagg
     5161 agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca acgacagatg
     5221 tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt ttgtctgtct
     5281 ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg tccttgtata
     5341 acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatg gcttctcctc
     5401 ttggactgat agatgttgta gtaggaagcg tcagcacgct gcttgtattg cttatcaact
     5461 ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggctgtgaca gcatgcgtat
     5521 ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa ctaccttttt
     5581 tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc ggtgggataa
     5641 ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt taatttaaaa
     5701 tatggcagcg tggaagagac tttgagtctt cttccatgct gcttttttga ttaatttgta
     5761 tgaagaaatc gatttagccg ttttttcatc aagataccga tataagctgc tccagttgcc
     5821 ttgattgctt ccggcagtaa aaacggcagt agtccactgg caaatgcagt tgtaaaagta
     5881 agatcaccac ttattttcag ccagatcgtt ccgcagatca atgcagcaag agagcccatt
     5941 agattggcaa agattgcata taagtaatga gcatccgttc tttcgatcat ccaaccagtt
     6001 atccacgttt gtaagagaaa tccaatcaaa tatccgccag ttggcccaaa taaaacagct
     6061 atacctccag tcatccctga aaatacagga agaccaatca agccaagtaa aagataaact
     6121 acaacagata tcaatcctag ttttcttccg agaaaagttg ctgttaatcc aatagcaaat
     6181 gtttgccccg tcaaaggaac caagccaaaa ggaatggtca tctgagaaag gacagaaata
     6241 atcgcggcaa attccgcagc aagtattaat tctttcaatg ttcggttcat aatgccctct
     6301 tttcgttaac ttaatatttt tatttggtta acgaaattgt aaaacaaagt gattactttg
     6361 taaatagaaa atataaaaaa agaccagcta tccaatagat agcggtcttt ttcaagaaag
     6421 cttttgaaat tataagcttt ggttgtagta ttcaacgaca agagcttcgt cgatttctgg
     6481 atatagttcg tcacgttctg gtaaacgagt caatgaacct tccaattttt cagcgtcaaa
     6541 gcttacgaat gctgggcgtc caacagttgc ttctacagct tcttttactg ttacgatgtt
     6601 ttgtgatttt tcacgaactg agatcacttg acccacttct acgtggtatg aagggatatc
     6661 aacgcgtttg ccatcaacta aaatgtgacc atggttaact aattgacgtg cttgacgacg
     6721 agtcgttgca agtcctaaac ggtaaacaac attgtctaaa cgttgttcta gtaagatcat
     6781 gaagttaaca ccatgtttac cttctttgat tttgctagct tttacgaaca agttacggaa
     6841 ttgacgttcg ttcattccgt acatgtgacg aagtttttgt ttttcagtta attgcatgcc
     6901 gtattctgat tgtttgccac ggctgttagg tccatgttga cctggtgcgt aagggcgacg
     6961 tgctaattct tttcctgtgc ctgacaatga gatgcctaag cgacgagaaa ttttccatga
     7021 tggtcctgta taacgagaca ttaaaatttc ctccaatgaa tataagtttt tggagtaaaa
     7081 taatccgttg aaaaattcat aatcatatag tttgttcttc aatcttcacc tttgcagccg
     7141 cggttacaca atcgaacccg agacggggca acaaactagg gatgagttat tatttttctg
     7201 ctgcattatt ttacacaaac tacagtatat gatagatcat ccacgaatgt caagataatt
     7261 agtacataaa aacgctttac aaacctaggc tgtctttata aaatgagagg gcatgtgcaa
     7321 atggaaaaaa gcgaaggaag tttaaggaga aaatcaatga atccggtatc accattattt
     7381 gagaaaatcg atgcttcaat tgaaaagaag gcgaatcttt ttgaaaacag cttttcacgt
     7441 tacgctgttc gagcggttct cgcctgtttg tttttgactt taggaacggc tgtggcattt
     7501 gcaatcgcga tgaaggggga agatatcgcg catggtctag gaaagatttt atacgcattt
     7561 atgttcagct ggggtctggt catgattcta tacatgaacg ctgaattagg aacatcgaat
     7621 atgctttata tgaccgtggg tgtttttcgg cagagacttg ctttaagtaa ggcagccaag
     7681 attctatttg cctgtatttt tttcaatatg attggcggta tattatgtgg atttcttatt
     7741 tctatgacta cgccatttca agatttaacg ttggacaact ttatgttaga atcaatagca
     7801 gtaaaactag caaaacctgc agcaacgatt ttggtggaag ggatgtttgc gaatatcgtt
     7861 gtcaataccg ctgtactgat cagcatgcgg atgaaagagg atgccgggaa agtgatcaca
     7921 gtcgtattca tcattttcat ttttgctttt ttaggatatg aacacgtgat agcgaatttt
     7981 cctgccttta cgcttgctta ctttgcttct caaggacaga cggatggaat gacagtcagc
     8041 aatgtgttac ataatttgtt ttttgctttg gcaggaaatt atattggtgg cggtttagta
     8101 atgggactgg gatatgcttg gctgaatcag tcaaaatcat cctatgtgga ctaaaaagtc
     8161 gttcattaaa ggggaagtgc catggaagtt ttagtttttc caaaaaatga ggaaaaagcg
     8221 ttacaaatgg aaaagtacat gaaaaatctt ttttcctttg ctggagtacc taagccggaa
     8281 cgcgctcttt tacaacaacc gttactggat gagagcaaaa atttatctat ttctgaagtt
     8341 ttagcttttg tttattatta ttatgaacaa atcgaaagag agtatcagta tgtagcgatc
     8401 gatttagtcc agaaaaacat caaacggctt acatttgaag acttagtcgt attgttgcca
     8461 ctagttcaga aaaaagaatg gtgggacagc atcgactctt ggagaaaagt atacagcaca
     8521 tggtgtaaag cctacccaga gcagcgtgaa gaagtctttc aattgtttga aaaaaaggaa
     8581 gatttttggc tacgtcgaat agcaatcacg ttgcaattag gttataaaca gcaaacagac
     8641 acaggactat tgatccgagc aatagacgaa gatcgtcaaa caaaggaatt ctttatccaa
     8701 aaagcaattg gttgggcatt acgcgattac agtaagaccg atgccgattg ggtaaaaaga
     8761 cagctgacaa cagatttgag taagttagct gttcgagagg caagtaagta tttataagaa
     8821 agcgagacga caagagatga gacgcaaatt atttgctttt gatattgatg gaacgttgtt
     8881 agattcaaac aaacaagctt tagatagcac gagagaagca ttggaaaaat tacgcaaagc
     8941 cggtcatctg gtcacgattg ctacagggcg gagccgattt catgcgcaag agatcattcg
     9001 cgatcttgat tttacgaact acattttatg taatggagcg gccggttttt tagaccatga
     9061 acaagtatat aagaatttac tagatcgcga ccagttggat gcgttcgttc aagaagcgca
     9121 tcatgagcag atcgatacag cttttgtcag cttagacaat atcaagcgtt cttcttccaa
     9181 tgatgtaacg gtgatggaag aagcgatgca ttctttcggt gctgtcttgc ctgaattaga
     9241 tcagtttttt gcagaggaac aagaaatata ccaagcgtta gcatttttcg atcaaacata
     9301 tgatgggaag ttctcttcat atgataaaat tcgatttgtc cgctggcatg agaacagtgt
     9361 agatgtcgta cctaaaaatg gttctaaagc agcgacaatt ctgcacttag cagaacaagt
     9421 aggcatccct catgaagaca tcatcagctt tggcgatgga caaaacgacc gtgaaatgtt
     9481 gcgcatgtca ggaattggga tagctatggg gaatgctgtg ccagaagtac aagcagaagc
     9541 taagatggtc actgatacaa acgatcagga tggaatttgg aaggcattga aagagttaag
     9601 cgtgatttaa aagaggttgt gaaaaagctg ttctgcatca agtaataagc ccagccaaac
     9661 aaaaatagct cctcctattt ttcgtttttc tgggcttatt accgcagatg caagcttttg
     9721 aacaccgttt aaggaagagg tcgtgacaga agtggtcagt ttcaagaaat aagaagggat
     9781 ttacgaaaat tgcttctcaa atttttgtaa aattacggct tatttctgaa gaagtagatg
     9841 aaacaacgtt tattcgggaa atgggactgt gacaagatgt ttgtcatagt cccatttttt
     9901 tattttattt ttcaaaacta agaaatacca ggaaagagtt ccttttgttt gaccttttct
     9961 ttcttattga aaacaactat aatatagagt gatgttaaaa gagaagagga atttagtgtg
    10021 cgggtattgt ttattggtga tgtagtaggt tcaatgggac gagagatgat tactgaatat
    10081 cttcctagat tgaagaagaa gtaccgtccg caagtaacga ttgtaaatgg tgagaatgcc
    10141 gcttctggac gtggaatcac agaaaaaatc tataaaaaat ttttgcaaga cggcgtagat
    10201 gtcgtgacga tggggaatca tacgtgggac aaccgcggaa tctttgagtt tgtaaatgaa
    10261 gctaaaaaga tggttcgtcc ggcaaatttt ccggaaggga cgcctggaca agggatggtc
    10321 tttgtcaaag tgaaccaagt cgaactggca gtgatcaaca tgcaggctcg ttcttttatg
    10381 gtggacttgg atgatccttt tcgtaaaatg aatgagctgg tagaagaagc cagaaaacgt
    10441 acgccgatca tttttgtaga ttttcatgga gaaacaacaa gtgaaaaaca agcaatgggc
    10501 tggtttttag atgggaaagt ttcagcagtg gtcggtacac atacacacgt tcagacaaat
    10561 gatgcgcgaa ttttgccaag aggaacagcg tatctgacag acgttgggat gacaggtccc
    10621 tatgatggta ttttgggtat gcgcagagaa cctgtaatcg aaaagtttct gaccgcatta
    10681 ccaaaacgtt ttgaagttgt ggaacaaggc cgatcgattt taagcggctg tatcttggaa
    10741 ttggatgata cgacaggtca tgcaaaaaaa atccagctga ttcagatcaa cgatgatcga
    10801 ccgttcatgg agtagaaaga gaggcagttt tttgcagcag gagccggaaa ttcaaaaaga
    10861 agtaaggaaa ctgacaaaac ttttaagaga gaatgaaacg atcattcgct acaaagaact
    10921 tgaagaaaag atccagcaaa accagtattt agctgagttg agagaaaaaa tcaaacaagc
    10981 gcaaaaagat gctgttcatt ttgcccacta tgacaaacca gcagcagaac aagaagcaat
    11041 caaacaagcg gatcaattca tgcaagaatt tgatcagcat ccgcttgttg tagcttatag
    11101 aaaacagctg ttggaagcag atgatctgct tcatcattta acaactatga tacaagaaga
    11161 aatcaatgga caaatagagg aggaaaaaca tgcctcaaaa aactaaaaat actcctatga
    11221 tggaacaata tctaagcatc aaggcccaat accaagatgc ttttttgttc tatcgtttag
    11281 gagactttta tgaactattt tacgaagatg cgatcaaagc gtcacagctt ttggaattaa
    11341 cgttgacgag cagaaatcga aatgcagaag aacctattcc tatgtgcggt gtacctcacc
    11401 atgcagcaca aggatatatc gatgccttga ttgaaaaagg ctataaagta gcgatttgcg
    11461 agcaggtaga agatccaaag acgactaaag ggatggtcaa gcgcgaagtg atccagctag
    11521 tcactcctgg tacagtcatg gacagcaaag gattgtcggc aaaagacaac aattttttaa
    11581 cagctgtagt tagtcaagga aatgaatttg gctttgctta tgccgattta agtacgggag
    11641 aattgaagac ggctcggttg catgatgaag aagctgtttt aaatgaagcc tctgcattgc
    11701 agacaaaaga actcgtatta ggcagtgaga tcactgatac ccttcgtgaa cagctaagcg
    11761 ggcgtttagg gctagtcttt tctcaacaaa atgagatgga agaaaacgcg gaatttagct
    11821 ttttgacaag cgaactagtc gatccgctga aaaaagaagc aaccggcaaa ctattgactt
    11881 atttatcggt gacacaaaag cggagtttag ctcatatcca aaaagcagta gaataccaac
    11941 cagatcattt tttgaaaatg gattattatt caaaattcaa cctagaatta agccaatcta
    12001 ttcggactgg gaaaaagcac ggcactcttc tatggttgtt agatgaaaca aaaacagcaa
    12061 tgggtggccg tttactaaaa caatggctag accgtccgct tatccaggca aaacaaatta
    12121 aagcaagaca agaaatggtc gcttctttgt tagacagcta ttttgaacgt atcgatttgc
    12181 agtcagcatt aacgaaagtc tatgatctgg aacgcctagc aggtcgagta gctttcggta
    12241 atgtcaatgg gcgagacttg atccagctga aaaattcttt agaacaagtg ccaatgatca
    12301 gagggttgat tgaaggaatc gatcaaggtc aatggcaaag tctgctttca gaaatgcagc
    12361 cgatggacca tctcgtccaa ttgatcgacc aagctatcca agatgatccg ccgttacaaa
    12421 taacggaagg aaacatcatc aaagatggat tcaatgagca gctcgatatg taccgttctg
    12481 ctatgagaaa cggaaagaaa tggttggcag aacttgaagc aaaagagcgt caagaaacag
    12541 gaatcaaaaa cttaaaaatc ggatttaacc gcgttttcgg ttattatata gaaatcacaa
    12601 aagcaaatct tggaaatgca gaactggaga agtatgaacg aaaacaaact ttagcaaatg
    12661 cagagcgttt catcacacca gaactaaaag aattggagac acaaatcctc gaagcagaag
    12721 aaaaatctgt tgatttagaa tatcagttgt ttttagctgt gagagaagaa gtcaaaaagg
    12781 ctatccagcc gctacaagta cttgcaaaag caatcagtgc agtagatgtg ctacaaagtt
    12841 ttgcaacgat cagcgaacgc tatcagtatg ttcggccaga acttgtcagt gacaagcatc
    12901 agcttctgat caaagacgga cgacatcctg tagtagaaaa agtattagga catcaggaat
    12961 atatccctaa cagcgtagaa atggcagaag acgaaatgat cttgctgatt acaggaccga
    13021 atatgtctgg taaaagtacg tatatgcgac agttggcatt aactgttctg atggcgcaaa
    13081 tgggctgttt cgttccggca aaacaagcag ttttacctat ttttgatcgg atttttaccc
    13141 gaattggtgc aagtgacgac ttaattgctg gacaaagtac ttttatggta gagatgatgg
    13201 aagccaatca agccctgcgt tatgctacgc caaacagttt gattttgttt gacgaactag
    13261 ggcgaggaac agcaacctat gacggaatgg cgttggccca agcaatcatc gaatatatcc
    13321 atcgtcatgt acaagcaaaa acactctttt ctactcatta tcatgaactg acggtacttg
    13381 agaaagaact tccgcagctt aaaaatgtcc atgttggagc cgttgaaaaa gacggagaag
    13441 ttgtcttctt gcataaaatg atggatggac ctgcggataa aagctacggc atacatgtgg
    13501 caaagatcgc aggattgcct tccaatctac tagaacgagc agcagatatc ctgcatacgc
    13561 tagaaaatgg cgataacgga caccatactg ttccagctga agtagaagag aaaactaaaa
    13621 cagccaaaca ggagcaaata aaaccacctg cagaaaaagt aaatcaagac catatacaag
    13681 accgtgtaaa agaagaaaac caacagcttt ctttattcag tgaattatcc gaaaatgaaa
    13741 cagaagtcat tgaatcactg aagcaaataa atttactaga aatgacgcca atggatgcac
    13801 taaacacatt atatgaatta caaaaacaac tgtaggaaga aggcggatat cgatggccaa
    13861 aatccaagaa ttatctgaac gtttagctaa tcagatcgct gcgggagaag tagtggagcg
    13921 accagcttcc gttgtgaaag aattagtgga aaatgcaatt gacgccggca gtactcagat
    13981 tgatatctta gttgaagaag cgggactgcg gacgatccaa gtgattgata acggagaagg
    14041 cattttggca gacgacgtag agaatgcctt caagcggcat gcgacaagta agatccataa
    14101 tcgcgacgat ctttttcgca tacgaacgct gggctttcga ggggaagctt tgccaagtat
    14161 tgcctctgtt tctgaaatga ctctggaaac ggcgaatcag gaagaaaaac aaggcaccta
    14221 tctgtcgctg aaaggcggag aagttattga gcatcgacca gcaccactgc gtcaaggaac
    14281 gaagattacc gtcagcaatc tgtttttcaa tacgccagcc cgcttgaaat acgtgaaaac
    14341 actgcacaca gaattagcga acatcgggga catcgtcaat cgactggcat tgagtcatcc
    14401 atcgattgct tttcgtttag tacatgatgg aaacaaaatg ctggcaactg ctggaaatgg
    14461 cgatctgaaa caaacaattg cgggaatcta cggattatct accgcaaaaa aaatgctgaa
    14521 aatcgaagga aaagatctag attttgagtt atttggctat gtttcattgc ctgaggtgac
    14581 tcgtgccagt aggaattact tatcgacgat catcaatgga cgttttatca agaacttttc
    14641 cttgaataaa gcaatcgttg caggttatgg gtcaaaatta atggtcggtc gtttccctat
    14701 tgctgtattg gaaatcaaaa tggacccgct tttagtagat gtaaatgttc atccgacgaa
    14761 gcaggaagtc cggctgtcga aggaaaaaga actaacagag ctaattagta acgccattca
    14821 aaaagcattg tctcaagaaa atctgattcc aagtgcagcg gataatctac ggtttaagaa
    14881 aaaagtacct gatcagccta aaacagaaca aatcgaaatc gatttgtcct acgcttttga
    14941 agaagaaaaa cctaaaaagc caagtagttt gaattttgat cgagagacag gtaccttttt
    15001 cgtagaagaa ccaaagaaca catttgttga cagaattgtt gataagtctg tggaaaatgt
    15061 tgaaaactat tctggttttt ctgaagaaaa caccacttat ccagaaaaaa cagcgaaaac
    15121 ggtggataag tttggtgaaa atgttgataa ctttgtagaa aaaacagtgg aaaaatctga
    15181 aaagaaagaa tttcccgttg tggataacat ggagaaaaca aatgaagaac aagcagataa
    15241 atccgtagaa gaggatgaaa agatttacga ggaagaattg acactccatc ctgaatttga
    15301 tttcactcaa ggaaacgaca aaacattaga acaagccatc aagaaattag aaacggaaaa
    15361 accagtacaa cgttttcctc agttggaata ttttggacag atgcacggaa cgtatctttt
    15421 tgcccaaagt agcgatgggt tgtacatcat tgatcagcat gctgcccaag agcgcattaa
    15481 gtatgaatat ttccgggaaa aaattgggga agtatctgat gatctgcaag aattgcttgt
    15541 tccatttgtg ttagactatc caaacagtga tgctttgaaa ttaaaagagc aaaaacactt
    15601 gctagaagat gcagggattt atttagaaga atttggtcaa aacagcttta ttgtacgggc
    15661 ccatcctact tggtatccca atggagaaga agaatcgatt attcgggaga tgatcgatat
    15721 gctgctgaca actggaagtg tgagtgtaaa aaaattccgt gaagcaacag caatcatgat
    15781 gagttgcaaa cgatcgatta aagccaacca ttatttgaat gaagcacaag cacgtgtgct
    15841 gctgaaggat ctggcacgat gcgaaaatcc tttcaattgt ccccatggcc gtccagtatt
    15901 gattcacttt accaattcgg atatggaaag gatgttcaaa cgaatccaag acccgcatta
    15961 agtcaaagag aagagggaaa acatgaagat tgtattagca tcccaatcac caagaagaaa
    16021 agagttattg gctcatatcg tttcagaatt cgagattgaa cctgctgata tcgatgaaac
    16081 gccatttcca gacgaacagc ccgtcgatta tgttcgtaga atggctgaag aaaaagcacg
    16141 aattgtctgg gaacaagatg aaaaagaaga ccatttagtg attgccagtg acacgactgt
    16201 tgttctcaat caagagatca tgggtaaacc agaagatctg gctgaggctg aattcatgct
    16261 gaaaaaacta agcggggaaa cacatactgt atatacagct gttgttttaa aaacaaagga
    16321 acgagaagaa cggatactag cagaagcaca tgtcactttt tatcctttga cagacgaaga
    16381 gatcaaacgc tacttggaga cgggggatta tgcggataag gcaggggcat acggcatcca
    16441 aaatgctgcg agtgtattcg tcaaagaaat ttctggagat tattacagta tcgtaggatt
    16501 tccgattggt gctgtcaatc aggcactgaa aacattcatt taaacaaaaa agcggactat
    16561 ctgattttcg attgaaaatc catgatagcc c
//
LOCUS       DAFEPQ010000056        16075 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.058, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000056
VERSION     DAFEPQ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.058"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            59..174
                     /gene="rrf"
                     /locus_tag="LP543_001975"
     rRNA            59..174
                     /gene="rrf"
                     /locus_tag="LP543_001975"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(227..697)
                     /locus_tag="LP543_001976"
     CDS             complement(227..697)
                     /locus_tag="LP543_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBL2322138.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(856..1314)
                     /locus_tag="LP543_001977"
     CDS             complement(856..1314)
                     /locus_tag="LP543_001977"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBL2322139.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1485..2630)
                     /locus_tag="LP543_001978"
     CDS             complement(1485..2630)
                     /locus_tag="LP543_001978"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBL2322140.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2643..3560)
                     /locus_tag="LP543_001979"
     CDS             complement(2643..3560)
                     /locus_tag="LP543_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBL2322141.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPNGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3988..4551
                     /gene="efp"
                     /locus_tag="LP543_001980"
     CDS             3988..4551
                     /gene="efp"
                     /locus_tag="LP543_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBL2322142.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4652..4822
                     /locus_tag="LP543_001981"
     CDS             4652..4822
                     /locus_tag="LP543_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322143.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            5037..6296
                     /gene="lysA"
                     /locus_tag="LP543_001982"
     CDS             5037..6296
                     /gene="lysA"
                     /locus_tag="LP543_001982"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBL2322144.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6283..7083
                     /gene="dapF"
                     /locus_tag="LP543_001983"
     CDS             6283..7083
                     /gene="dapF"
                     /locus_tag="LP543_001983"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBL2322145.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            7086..7967
                     /locus_tag="LP543_001984"
     CDS             7086..7967
                     /locus_tag="LP543_001984"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBL2322146.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7964..8680
                     /locus_tag="LP543_001985"
     CDS             7964..8680
                     /locus_tag="LP543_001985"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBL2322147.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8686..9381
                     /gene="dapD"
                     /locus_tag="LP543_001986"
     CDS             8686..9381
                     /gene="dapD"
                     /locus_tag="LP543_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBL2322148.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9397..10398
                     /locus_tag="LP543_001987"
     CDS             9397..10398
                     /locus_tag="LP543_001987"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBL2322149.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10395..11756
                     /locus_tag="LP543_001988"
     CDS             10395..11756
                     /locus_tag="LP543_001988"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBL2322150.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11716..12867
                     /locus_tag="LP543_001989"
     CDS             11716..12867
                     /locus_tag="LP543_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBL2322151.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12842..14011
                     /locus_tag="LP543_001990"
     CDS             12842..14011
                     /locus_tag="LP543_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL2322152.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14186..15565
                     /locus_tag="LP543_001991"
     CDS             14186..15565
                     /locus_tag="LP543_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL2322153.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15846..16075
                     /locus_tag="LP543_001992"
                     /pseudo
     CDS             15846..16075
                     /locus_tag="LP543_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5257 a         2839 c         3428 g         4551 t
ORIGIN      
        1 tgacttcaaa tccagttttg agtgaacaaa atttactcaa taaaagataa caccacagtg
       61 tggtggcgat agcgagaagg atacacctgt aaccatgccg aacacagaag ttaagcttct
      121 tagcgccgat tgtagtgagg ggttgcccct tgtgagagta ggacgtcgcc acgcaaaggc
      181 aaaaagatga actagaaata gttcatcttt ttttattttt attaagttaa agaacggatt
      241 caattgcagc ggtcattttc aatggagaag tttgtggtgc aaatcgttcg attactttgc
      301 catttctatc aatcaaaaat ttagtaaaat tccattttat tgctttagtg acagtaccag
      361 gagcttcatt tttaagataa gtaaaaagag gatctgtatt ttctccattt acgtcaatca
      421 attgatgcat agggaaggta acgccataag tggttttaca agcttctgct gcttcagtcg
      481 ctgaatctag ctcttgttta aattgattgg aaggaaagcc taagataacc agaccttgtg
      541 tctgataagc ttggtaaagt tcttctaatt gtttaaattg aggtgctaga ccgcattttg
      601 ttgcagtgtt aacaatgatt atcggtttgc ctgcatattt tttcaatgaa tagacagtgc
      661 cgtcttctaa agtagtttca aaattataaa tagacataaa gttttctcct ttctatttta
      721 tataataaaa agagcgctaa actgaaaact agctatttgt tttcccgttt agaactcttt
      781 tcagacaata aatatttaat aaaatacgaa gataagatag ctatattgct tctcatcttt
      841 acgttctttt agaaactatg cttctacttc agaccattca tcgcgttttg ctaaaaattc
      901 acgagccaaa gcttgtgcac cttctagtgt atggctggca gcccagccac attgtgtttc
      961 attgcttgca ggtacttcag tagcttcaag aacatctttc attgttgctt caagaacatc
     1021 taaaatgtct tcgtagcttt catgattcaa tacagtcaag taaaagcctg tttggcatcc
     1081 cattggagac cagtctacga tgtaatctgc gtgattgcgg attagttcgg cagtcaagtg
     1141 ctcaagtgaa tgtagacttg gcatgtccat gtgttcttta tttggttgtt taaagcggac
     1201 gtcgtattta atgatgacat cgccatgcgc acccgttttt cgatcagcta cacgtacata
     1261 aggtgctttg acttttgtat ggtcaagatt aaaactttcg acattcattc tcatttgtaa
     1321 gactcctatc tgtttgttca ttcgttttga tgcttttcac taaagaatta tttagtgaaa
     1381 tttacaaaaa cgtctttcta cttgattcta acacttatcg tttccaaggt gtagcaatct
     1441 gaaatatttt ttagattttt ctgctaagtc gtttattggg acttctagtt cttgattctg
     1501 tcaaatgcct gagctaaatc gtttatgaga tcctgaccat actcaattcc tacagaaaga
     1561 cggatcaagc cgtcctttat gccagcctct tctcgttttt cttttggaat agacgcatga
     1621 gtcatgacag aaggaatttc cactaaactt tctacgccac ctaaactttc tgctaaagta
     1681 aatagttgga gtgattcaac aaatggaata gcagattctt cattttttaa agtaaaagaa
     1741 atcattccgc taaagccgct catctgctgc ttagcaactc catggagttc atgatcgggt
     1801 aatcctggat agtagacctt ttctactgct ggatgagaaa atagaaaatc agctaccaca
     1861 aaagcatttt tttgatgttc ctccatacga acacttagtg tcttgattcc gcgctgaagt
     1921 aaccagctat cttggggacc aagtacggct ccgatagcat tttggtaaaa tccgatctga
     1981 tcggctagat ctttatggtt agaagtcact aaaccagcta ctacatcgct gtgtccgcct
     2041 agatatttgg taccgctatg gacaacgata tcagaaccga gatctaaagg tctttgaaaa
     2101 taaggagtag caaatgtatt gtcagcaata actatcaatc catgttgatg agccagagtg
     2161 gcactttttt ctaaatcagt aatctttaga agcggattgc taggtgtttc aagataaagt
     2221 gcttttgtat tgggtttgat ggattgttct attttatcaa gattactcgt atctatgatc
     2281 gtgtactcga gaccattttt agtaagaact ttgtcaaata gacggaatgt tcctccatag
     2341 acatcatctc ctaataagat atgatcgcca gcttgaaaaa gagaaaaaac ggcatgaatc
     2401 ccagaaagac cagaagaaaa agcgaatcct cgaacaccgc cttccaaatc agcaatcagt
     2461 tcttccaatg caaaacgagt aggattacca gatcttgaat attcatattg tttaggctgg
     2521 ccgacgccat cttgccgata agtggatgtt tgataaatag gtacactgac agctcctgtt
     2581 gtgggatctt cactgatgcc tccgtggatc aattttgttt gaatgtgcat gtttctttcc
     2641 tcctataagt aaatgttttt tgataaatag cggtctgctg cgtctggaaa aatcgtaacg
     2701 acacgatgac caggtggtaa acgacgaatt tctcttaatg ctgctgcaaa agccgcacca
     2761 ctcgaacttc caactaatag tccttgttct cgtgctaact gcctagtata gttaaaccct
     2821 tctacatcag agattgtctc gatctgattg attgaaaggg gagaaagaaa cggcggaatg
     2881 aattctacac cgataccctc aatttcgtga ggggctggat caccaccatt taacacagag
     2941 ccctctggtt ctacaccgat tattcgaatg tcgggatatt tttctttcaa aaaggtagaa
     3001 gttccagcaa aggttccacc actcccaatt cctgctacaa aactatggat attttctttg
     3061 atttcatgaa agatttctgg ccctaacgta tgatagtaag cagcaggatt atctttattt
     3121 tcaaattgaa gaggcagata gctgtttgat atctcctctg caagtttttt acttttgctg
     3181 atggcaccga tgattccttg ctcactagga gaatggatga tctttgctcc caatgctttc
     3241 atcaattgtt gtttttctaa gctaaacttc tctggaacaa caaaaaccgt tcttaatttg
     3301 tatgtgagtg cggccagagc gatcccaatt cctgtatttc cggcagttgg ttcgatgatc
     3361 gtcgtttttt cattgatttt tccttgggca aatccttctt tgatcaaata actgcccaat
     3421 ctatctttga tacttcctcc aggatttaaa tattctagtt ttgcataaat agcagaacca
     3481 ttaggcatag aatagtgctg attgtcaaag cgataaagcg gtgtggaacc aatagcttct
     3541 gtaattgtcg agataatcat gtgggcctcc taaaaaaaga taataaaaaa aggagctgac
     3601 tattaaatca gctccttgaa agacatagag aaaaattgag tagataaaaa gataagttac
     3661 ctggtactca ttctatgaag ggcaattaat agacatgaca aatagtatgc tgcttttatg
     3721 caccatacaa cagcaacgat tcaattgttt ttggttgctg actgtcatgt gtatccctcc
     3781 aagtaaaatt tttattaacg atagcatgaa aaaaaataaa agtcaaaggc ttgaaagaaa
     3841 aaaatatttc gatagcatcg tttacccgtg tttatttttc tgacaaatag gcatgagacc
     3901 ttaaaagata gagaatactc tagttttctg gccggttgtc tgatacaata aagttgatta
     3961 tgataaagaa aagaaggagc aaaaataatg atctcagcaa gtgatttaag agccggtatg
     4021 actttcgtac aagacggcaa actgattaaa gttctggatg caagccacca taaacctggt
     4081 aaaggaaata cagttatgcg tatgaaactg aaagatgtcc gttcaggtgc aacatacgac
     4141 acaacgttca gaccagaaga aaaatttgaa aaagcacata tcgacacaaa acctgtacaa
     4201 tatttgtaca cgatggatga tacagctttc ttcatggatt tggaaacata tgagcaatat
     4261 gaaattcctg tagaaacagt agaagaagaa atgaaatata tcctagaaaa catggaagta
     4321 aaaatccaat ttttcggtag cgaagtcatc ggcgttcaat taccaacaac tgtcgtatta
     4381 cgtgttgtgg aaacacaacc ttccatcaaa ggtgcaacag tcactggttc aggtaagcca
     4441 gcgacaatgg aaactggctt agtagtcaat gttcctgact tcatcgaaac agatgaattg
     4501 ttggaaatca atacagcaga aggttcttat gtaaaacgtg cgtcaaaata attcgctaac
     4561 ggattgataa gaatttgaaa acagagcttg acctcttgaa aggcagatca tcggatctgc
     4621 ctttcaagag gttttttgtg gaatcagcaa attgaaaaat aaaaaagtat ttgattttat
     4681 ttatattttg aaagatgaat ggcaaaagct agagaaagaa aaaactgcac ttatttatga
     4741 agaaattgaa atgattcata aaattgaaaa tatccgaact attccgaact atattgaagc
     4801 gaaaatattt tttcatcact aaatagtgtt gaatttatgt ttttatgtat aaatagcttc
     4861 taatagttcg ggtattttgt ttttataacc atttttattc ttatttttaa aaaaatattt
     4921 cgaaatatac tagacaattc agaaaatttt gtttaatatt attccaataa aattcaaatc
     4981 tttatcaaat gaaattccaa taaaaacgga aaaaaagtga aaagaggaat gaaaaaatga
     5041 cagaattgat aattggcgga gtcacagcaa gcacattggc agaaaagtat aagacacctc
     5101 tgtatgtgta tgatgaagaa aagatgagac aaacgatgct gtcattcaag cgaggctttg
     5161 tatcgaaacg ttttgataca aaagtgcttt acgcctctaa agcattccaa acgattgaga
     5221 tgctgcattt agttcgtgaa tatggattcg ggctagatgt tgtcagtggt ggggaaatct
     5281 ataccgcttt gcaagctgat tttcctagag atactatcta cttccatggt aacaacaaga
     5341 cgccagagga actgatctat gcacttgaaa atgatttgtt gcatattgtt gccgataatt
     5401 tgatggaagt tgaattatta gcagaattaa gccaacgcta tttaaaaaaa atttgtatca
     5461 tgcttcggtt gaatgtcgga gtagaagcac atactcatga atatatcgtc actgcgcata
     5521 ttgattcaaa attcggcatg agctatgaaa gtaaagagtg ccaacaagcc cttcagttga
     5581 taaaagaaag cagctatcta gagttagaag gatttcatgc tcatatcggg tcacaaattt
     5641 ttgatgttac tgcttggtta gctgaaatcg acaaattgac gaattactta accgattttg
     5701 accaacctct ttccttgaat cttggcggag ggtttgggat tcgttataca caaaatgatc
     5761 agccgatccc agtcgaagag gcactaaaaa aactgattga atatacggaa gaagctttgg
     5821 aaaaacgaaa actgaccatt cgtcaattac tgatcgaacc gggtagaagt attgttggag
     5881 aagctggtac aacactatac acgattggtt tcatcaaaaa gacaccacat aaaaaatatt
     5941 actttgtaga tggcgggatg acagataata tccgtccagc tctgtatcag gcagaatacg
     6001 attgtgattt agcagcaaaa ctgtcggaag aaaaaacaga gaaagtgacg attgcaggaa
     6061 aaatgtgtga atcaggcgat gtggtaatta aagaagctta tttgccgaaa gcagaatcgg
     6121 gtgatctttt agctgtttat tccacgggag cttatggata ctcgatgagc agcaactaca
     6181 atcgagcgac acgaccagca gttgtttttg tcaataatgg ccaatcaaga ctgattgttc
     6241 gcagacaagc attttctgat ctactaaggg gggaagtttc ctatgatcct tcataaatat
     6301 aatggctgcg gaaatgattt tatcttatta gattacgcag aagacacaga ttatagtcaa
     6361 ttagccgctt ctttatgcac aaaagaaaaa tatgatatag acggattgat cgccgtaaaa
     6421 atcgatccgt tggaaatgat ctattacaac aaagatggca gccgcgcgcc gatgtgcgga
     6481 aatggcattc gctgttttgc tcgatacgta aatgatcaga acatgatgaa tgaacaggaa
     6541 ttcaacgtag agacattagc aggcacaaga aaaattacgg tgttggatcc ctcacctttt
     6601 tattgcagcg tagatatggg agtgccagat gattctccgg aaaaaatcaa agctaatcaa
     6661 acttatccaa taaaaaacca ggaaatagag atcaatggtg aaaaagtaac gatcactagt
     6721 ttatttttag gaacgattca taccgttgtt tttgttgaaa atgcgcgtga ggaagtctgg
     6781 agaaaaagag gtgaactgat ctgtcatcat cctttgttca aagaaaaaac caatgtcaac
     6841 tttgtagaag tagtcaatga acaagaattg atcgtccgta cctatgaaag aggagtaggt
     6901 tggacacttg cttgcggtac aggttgctgt gcctcttatg tggttgctaa ggaaactggg
     6961 aaaatttcag cagatcaggt agttgttcac ttggaacaag gagacttgat gatctctggt
     7021 gaggatgcga tacaaatggc tggcccagca gttcatgaat ggacaagaat attggaggaa
     7081 tgaagatgat aaaaggttcg atcgtggcgc tgatcacgcc gatgaatgaa gagggatcag
     7141 tagattatgc tggtttggaa aaattgatcc aattccattt agatgaacaa acagatggat
     7201 tattagtttt aggtacaaca ggagaatctt ctacgctcac acaatctgag gaggagcaga
     7261 tccttcaact gacagtaaag aaagtggctg gccgtgtgcc ggtgattgca ggagctggaa
     7321 cgaataatac gaaagaaacg attgaaaaag cgaagcattt tgcttctttg ggggcagacg
     7381 cacttttggt catcacccct tactacaaca aaacaagtga tgcaggacta gctgctcatt
     7441 ttacagcaat tgcagaagcc tcaccgattc cgcttatttt gtataatgtt ccttctagaa
     7501 caggcatgtc gattccaatc catgtattag taaatttagc agaacatcct aatatcattg
     7561 gtttgaaaga agcctcagga gatatggctt atgtgatgga tgccgctcgg ttgattggag
     7621 aagaattctt cctttacagt ggaaatgatg atttgatcct accagtaatg agtgtaggag
     7681 gaagcggtgt aataagtgta tgggcgaata tccaacctaa aatagtccat gaattagtaa
     7741 aagatacgca ggacggaaga tggcagcaag caaaagaaaa gcaattaaat gcacttgaac
     7801 tgatccatgc cttgttcagt gagaccaatc ctattcctgt caaagcagcc atgtcattat
     7861 tagacttacc ttccggacct ctacgcctgc ctttagtttc tctttcaaaa gagaaaaaga
     7921 aacaattagc tcagcttttg ttaaaagaaa ggacggttga aaaatgaaaa tcggactgat
     7981 tggcagtgga cagatgggaa atagagtcga agaagttgca caagaaagag gagatacggt
     8041 ccttctttta cggacggcac ctaaagaagc aactacgctt gcttttacgg aactgccaga
     8101 actgcttatt gatttttctc atccagataa tttagagaaa atcctttcgt atagcttatc
     8161 atatcaactt ccgttagtca ttggcacgac aggttataca caatcacagt tccaagaaat
     8221 aaaagaacat gccaaacatg tacctgtgat gtatagtgct aatttttcgt tcggcatcat
     8281 ggtgatgaat caactaatca agcaagcagc tgcaatgttg caaggctggc agatcgaact
     8341 gattgaaaaa catcatagtc gtaaaaaaga tgcaccatct ggtactgcaa atatgctgct
     8401 tcaaacaatc caagaagtgc aaaagctgca gcccgtatac aactggcaag ggaaaaaaag
     8461 agaagaaaac caaattggtg tacacagtat tcgtgcaggt tctttacctg gtgaacatga
     8521 tgtattgttt gctgctactg atgaaatatt tacgatcaaa catgaatctt tttctaatcg
     8581 aatctttgct gcaggcgcag tagaagcagc ggactggttg aaagatcagt caccaggtta
     8641 ttacaaatta gaagatttat taatggacaa aggggtgtaa aaaacgtgga agctcaagaa
     8701 atcatccgct acattcaaac ggcatctaaa aagacgcctg tgaaagtcta tctgaatgta
     8761 accgagccaa tcgaatttaa agaaagtaaa gtttttggtg aaggaagcag cttcgttgtt
     8821 tttggtgact atgagacgat tgctccggtt ttagaagctg aatcagaaaa aatcattgaa
     8881 atcgaaatcg aaactgctgc aagatacagc gcagttcctt tgctagatat taaaaaaatt
     8941 aatgctcgta tcgaaccagg tgctattatc agagatcaag tgtcaatcgg caacaatgcc
     9001 gtgatcatga tgggagcgat tatcaatatt ggtgcagtaa tcggtgaaaa cacgatgatt
     9061 gatatgggtg ctgtcttagg tggacgagcg acagtgggca aaaactgcca tattggtgca
     9121 ggagctgtct tagcaggtgt gatcgaacca gcatcggcca aaccggttat cgtagaagac
     9181 ggcgtactgg taggtgcgaa tgcagtgatt gttgaaggtg tccatatcgg aaaagatgca
     9241 gtcgtggcag caggagcagt ggttttagaa gatgtggcag cagaaactgt tgtcggtgga
     9301 atacctgcac gtgtattaaa aataagtgat gatcagacaa aagaaaatac agcattgatt
     9361 gctgcgttac gcgaattata gaggagatgg agaaaaatgc gcgaaataga tacctgtatt
     9421 gtgggggcaa ccggattagt cggccaaacg atgctcaaag tgttagaaga atatgatttt
     9481 ccagtaggac gattgaaatt attagcttca aaacgttcag ccggtaaagt ttgtgtattc
     9541 aagaaaaaga aacacactgt acaggaattg acagaaactt catttgaagg atatgattta
     9601 gcgctatttt ctgcaggtgc ttctgtatcc aaagagtttg cgccaatcgc acgaagtcag
     9661 ggactatttg tcatagataa ttcctcagca tggcgggaag attcggatat tccattaatc
     9721 gttccagagg tcaatctgga agactataca ttgaatcggc tgattgcaaa tccaaattgt
     9781 tcaaccattc aagcggtact tccgcttaaa gctttacaag aagcctttgg tatccagcga
     9841 gtcaattatt cgacctatca agcagtttct ggttccgggc aaaaaggcat tgaagatctt
     9901 catcgtacgc aaaatgacga ggaaccagtt ttgtatcctt ataatattag ccaaacggtg
     9961 attccggaaa tcgatcagcc tttggaaaat gggtatacaa aagaagaaca gaaaatgata
    10021 aatgagaccc gtaagattct gcatcagcct gctttgccag tatcagcaac ctgcgtacgt
    10081 gtgccaatcg aaaatggaca tggagtctct gtggcagttc aactgcaaaa agagtttacg
    10141 acggaagaaa ttagagagtg tctcagccag tttccaggga ttcaattaga agatgatcta
    10201 gcagcacata aatatccgac gagcattctt gctagaggaa cagatatggt ttatgttggg
    10261 aggatcagaa aagacacttc tttagagaac ggattgttgt tctatacgac tgccgataac
    10321 atacgcaaag gagccgctgc gaatgccgta cagattgctg cagctttaat gcagaaaata
    10381 gcggaggtgg caagatgagt aatacgctga aagtagcgaa attcggcgga acatcgatgt
    10441 ctcatgaaga gcaattcaaa aaagtccgta caattgttct ttcagatccc aatcgccaag
    10501 tgatcgttgt ttctgcttta ggcaaacgta cgtcagatga tgacaaagta acagacttac
    10561 tttatttgat ttatgcttac cttcagcatc atgttgattg gacacccctt tggaaaagga
    10621 tttgtgatcg tttcattcaa gtaagagatg ttcttgccct gcagttacct attgaagatg
    10681 aactagcacg catacagaaa caactggaag acgaaaagat aactgaagat tatttagtaa
    10741 gtagaggtga atatctgact gctcaactga tggctgagta tcttggttac cagtttattg
    10801 acgcaaaaga cctgattttc tttgactatg atgggaaaat cgatcaggaa aaaacagcac
    10861 aggaaatcac aaaagcattt aaaagatatc cgaaaatggt tgttccaggg ttttacgggg
    10921 cgaaccctgc aggaaaagaa aaattattgg gccgtggggg aagcgacatt accggcgcga
    10981 tactggcgaa gatgttgcaa gcggaaagtt atgagaattg gacggatgtt tcaggaatca
    11041 tgatggctga tccgcgaatc atcgatcatc ctaaaatcat cgaggaaatc tcttttagag
    11101 aactgagaga gatggcttat atgggggcta acgttcttca tgaagcagct gttttcccag
    11161 ttcaagaggc tgatatcccc atccaaataa aaaataccaa tgatcctttc gctcaaggga
    11221 caaaaatatc gaatcacgaa atagaaaagg aaaatggact gacaggaata gctggacgca
    11281 aggattttct ttccatcact ttattcaaac gtcatatgtc cgatgaaatc gggtttatct
    11341 ggaaagcaat gagcattttt gctaaacatg agatatcaat cgaacatatt ccttcaggga
    11401 tcgacaatat cggagtgatc gtctcagcag aagcgattgc cgataagtta tttctcatca
    11461 cgaaagaatt aaaagaaaca cttggcgtgg aagaaatcgt cgtgatcgaa gacttagctt
    11521 tgatctctgt cgttgggggt ccgcataaag aattgattgg attatctggg aaagtgttat
    11581 cgatattgaa caaattagaa attcgcacat cgatcttatc acaaggagca caggagttga
    11641 atctgatcat cggtgtaccg aataatcaat atgaaacagt agtgaagggt atctatgaag
    11701 ggatggtgaa tacggatgct gaaagaatat cgaatggcac tccatcaaat tcctgaaatc
    11761 gggtttcatg aattcaagac aaaaaaatat ttatatgaac aagtaaaaaa cagcgaggcc
    11821 gttatccatg aaatcggcga aacaggacta ctcctttatt ttgataatca gcaagaagaa
    11881 acgattgctt ttcgaacaga tatcgatgca ctgccagtca aagaagaaac gaatctctct
    11941 tttgcttcta agcatgaagg ttatatgcat gcatgtggtc atgacggaca tatggcgatg
    12001 cttttagggt tgactgactt tataactaaa cacattgagg atcttattta taatgtagta
    12061 ctgatctatc agccatcaga agaaatttcc ggtggcgcac aaagcgttat tcagtcagga
    12121 ttgttggata aatatgaagt acaagcaata tttggctttc atctatggcc tggactagct
    12181 aagggagaag ttttctctcg accaggacct ttgatggcac aaagcagtga aacagacatt
    12241 atcattaaag gccagtctgc acatattgct tctagtgaaa aaggaatcga tagtctggag
    12301 gcagctgtac gatttttgaa gaatgtttat gattttgacc ggcaattacc ggcagagtgg
    12361 aatcatctaa tgaaatttgg ccaaatccaa ggtggaacga tccgaaatgt tttagctgga
    12421 gaagttattg tatccggaag tatccgttct tacagttcag atatgcaaaa ttatttgaag
    12481 aggggattag accatttagc agaacagttt cactctacag ctactatttc ttttcgctat
    12541 aatgacggct atccagctgt aatcaaccat agaggcttat atgcagcact gttagattca
    12601 aacaaactac atgaattaga tgctccagtt cttcaagccg aagattttgg catatatacg
    12661 caacaatatc catgtgtatt tttcttttta ggtgtcggtg atacccctgc tttacatgat
    12721 ccgacgttcg attttgatat ggatgtttta gaaaatggtg tagaatggta caaaacgatt
    12781 ctttttacag aaaatcttct tgtaaaaatc gatagcagtt caaaagagga gcgagtaaat
    12841 aatgatgaat ttatccaatc gatttagtcc taggctttct agaatagaag tatcaaaaat
    12901 acgacaattc gatcagcaaa tatcgtcgat accagatgtg atcaaactca ccctgggaga
    12961 accggatttt ccaactcctg aacatgtcaa gcaagcagga atagcagcga ttgaagaaga
    13021 cttttctcac tataccggaa tgagaggatt agaggaactg cgggaagcgg cttgtatatt
    13081 tcagcaacag cgttatggat taacttatga tccgcaaacg gaagttttga cgacagttgg
    13141 tgcaacggaa gcgattgcct ctgcgttgtt atctgttttg gaagaaggag acaaggttct
    13201 tattccagcg cctgcttatt caggatatca gccattagtg gaactagctg gtgctgaatt
    13261 gatcccgata gatacttctg acacagggtt tgtctgtcag ccggaacaat ttgaacgagc
    13321 atttgaacaa tatggatcag ctgtcaaagc agtcatttta aactatccta acaatcctac
    13381 tgggacgaca ttgtctgcaa aacaattgaa agcaatcgct gaggttttaa aaaaatatcc
    13441 ggtatttgtt ataagtgatg aagtatatgc tgaattgact tatagtggaa cgcatatgtc
    13501 catagctact tatcttccag aacaaacaat cgtgataagc gggttatcca agtcacatgc
    13561 gatgactggt tggcgagtgg gctttatttt tgctcaaaaa gcccttattg acgaactaat
    13621 taaagtccat caatatctgg tgactgccgc tacaacgatg tcgcaaaaag ctgcggctga
    13681 agcgctaata aatggggtga aagatagcga gaatatgaaa gagcaatatc aaacacgaag
    13741 agactatctg gtccagcaat tggcgccttt aggatttgaa gtgacccagc caaacggtgc
    13801 attttacttg ttttgcaaac tcccagctgc cattcaaact gactcttggc agttttgtgt
    13861 agaattagcg gaacaggcaa aagttgcgtg tatcccagga attgcatttg gcccagaagg
    13921 agaaggttat atccggattt cttatgctag cagcatggaa aaattacatg aggcctgcaa
    13981 acggatcaaa gactttttaa cagaacagtg attcatagat tttatcaaaa aaacaatcgt
    14041 taattttgcg gtatattctg caaggagacg attgtttttt aaatttagtt caaatgaacg
    14101 cgtttctttt gattcataac taatgtaatg ttaagtttaa attgtttaga atgattttaa
    14161 aagaatattc aaggaggttt catcaatgga aaaaatcaaa gttgtcattg taggtgcatc
    14221 tcatggaggg catcaatcga tcttggaact attgacacga tacggtgaaa atgtagatat
    14281 cacgctattt gaagcaggag actatatttc cttcatgtca tgtggtatgg agctgtattt
    14341 agaagacaag gtaacagatg taaatgatgt acgtaatttc agaccagaaa actttccgca
    14401 atccaatgtt catattttaa ataatcacga agtaaaaacg atcgatgccg ataaaaaaac
    14461 agtaacggtt actagcgcat cagataacga aacaaaagaa tatccttatg acaaattgat
    14521 tttgagttct ggtgtcaaac caaacaatct gccagtacca ggagcagatc tagaaaatgt
    14581 ctacttgatg cgtggataca attgggcaac caaaatcaaa gaaaaattaa atgatccggc
    14641 tattaaaaaa atcgctgtcg taggtgcggg ttacattggg atcgaagcag cagaagcaag
    14701 ccgaaaagct gggaaagaag tcgtattgct tgatgtgatc gatagacctt taggcactta
    14761 tttagatgct gagatgacag atattttaga acaacaccta aatgaaaacg gtattgaagt
    14821 tttgacaaat gcaaaaatcg aagcttttac tggtaacgga aaagttgagg caatcaaaac
    14881 aagtgaaaaa gaaatcccag cagatctagt cattcaagct gccggtgtga aggcaaatac
    14941 agaatggtta aaagggatcg tcgacttaga tgaacgtggc tggatccaaa caaatgaata
    15001 cttgcaaacg aatcttccag atgtgtatgc agtaggagat gctacacttg cttattctat
    15061 cccagcacgc aaaaaaatgc cgatcgcttt agcgactgtt gcacgtagag aagcacgtta
    15121 tgtagtaaaa catctttttg aagaagtacc aaacaaaccg ttcggcggag tcgtaggttc
    15181 ctctgcatta agtgtctttg actatcattt tgcagcaagc ggattgaata gctttacagc
    15241 agcaaaagca ggagtagatg tacgagtgtc ttattatgaa gatacgttac gaccaaaata
    15301 tgtaccagaa gaacaaggaa atccaaaagt gtccatccaa ttattctcgg atccaatgac
    15361 ccatcaattg ctaggaggag cagtattatc tacctatgat gttacaggtc aaggaaatgt
    15421 attggcacta gcgatccaaa acaaactgac tttagaagaa ctagcagaag ctgacttctt
    15481 cttccagcca ggctttgacc gacaatggag catgttgaat ctagccgctc aacaagcgtt
    15541 aggagaagaa ccatttgtag aataagaaaa cgaaatattt gaaaagaggt tgtgaaaaag
    15601 ctgccttgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg tttttaactt
    15661 ggctggactt attaccgcag atgcaagctt ttgaacaccg tttattttca aaaaagggac
    15721 tgtgacacga tttgtgtacc gacccccaaa agttagacca aaaaatctaa cttttggagg
    15781 tcggtatttt ttatggttaa atatactttt gaacttaaat taaagatcgt tcatgattat
    15841 ttggatggca aaggcgattg gaccgtaaaa atccagatca agagattgaa gcggaaatgc
    15901 ttaaaattcg ggagaagcac aaggattatg gctgtttacg gatgaccaat gaactaagaa
    15961 accgcggttt ctctatcaat aaaaagaaag ttcagcgctt gatcaagaag ctaggaataa
    16021 aagtaacggc atacacgcgt aaatcacgtc gctacaattc ttatcgagga aaagt
//
LOCUS       DAFEPQ010000057        15852 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.059, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000057
VERSION     DAFEPQ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.059"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            292..1431
                     /locus_tag="LP543_001993"
     CDS             292..1431
                     /locus_tag="LP543_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBL2322154.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="LP543_001994"
     CDS             complement(1498..2073)
                     /locus_tag="LP543_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL2322155.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="LP543_001995"
     CDS             complement(2078..3052)
                     /locus_tag="LP543_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBL2322156.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="LP543_001996"
     CDS             3335..4303
                     /locus_tag="LP543_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL2322157.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            4416..4694
                     /locus_tag="LP543_001997"
     CDS             4416..4694
                     /locus_tag="LP543_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBL2322158.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="LP543_001998"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="LP543_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBL2322159.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            5151..5906
                     /locus_tag="LP543_001999"
     CDS             5151..5906
                     /locus_tag="LP543_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL2322160.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="LP543_002000"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="LP543_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBL2322161.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="LP543_002001"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="LP543_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBL2322162.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="LP543_002002"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="LP543_002002"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBL2322163.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="LP543_002003"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="LP543_002003"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBL2322164.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="LP543_002004"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="LP543_002004"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBL2322165.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="LP543_002005"
     CDS             10643..11185
                     /locus_tag="LP543_002005"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBL2322166.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            11208..12764
                     /locus_tag="LP543_002006"
     CDS             11208..12764
                     /locus_tag="LP543_002006"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBL2322167.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="LP543_002007"
     CDS             12778..13680
                     /locus_tag="LP543_002007"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBL2322168.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="LP543_002008"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="LP543_002008"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBL2322169.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="LP543_002009"
     CDS             15123..15545
                     /locus_tag="LP543_002009"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBL2322170.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         5264 a         2840 c         3419 g         4329 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatgag caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatc atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaagc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg gcatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacta attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attgcattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga ttgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttgtggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaaac gatcaaggta gtctgtacct gcctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccacgactta ttccttgagt ttccacagca gtgaagcttt
     2281 tgaaaccttc acagatggcc ccctcgataa aataagcaac agctaacgga tcattgatga
     2341 cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt gacagcagta ttcgaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg agattctcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggcgaacagt
     2581 taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt ttcccaatat
     2641 ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca atgactgatg
     2701 tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga ggttctgcct
     2761 gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca tgagtgtctt
     2821 gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca ggaatgtcta
     2881 aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct gcagggacat
     2941 tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca gctaatgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc gaatgtaaat
     3181 gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg gataatagtt
     3301 agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt tcattcttat
     3361 tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc gtttgatgga
     3481 ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag agtgtgaaag
     3541 cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga aactagctgg
     3601 tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac atgattaccg
     3661 aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagat cggctagatt taattgcctg gatccaactc atcttagatg aacagccgaa
     3841 tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga tggcaagtgg
     3901 tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg tttctgttta
     3961 tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa tggtcatgag
     4021 atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag catcagctac
     4081 gagacagctt ggcagcaacc atctgccttt attggtcatt catggtgaaa gagatcattt
     4141 tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata aagcactgct
     4201 gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatgaat taattttctc ttcttttttg
     4321 taagtaattc gaggataaat aattgtttat agatcaggag actgctaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa gaaaaaaatc
     4441 gtcttgcttt attcaatgat gaacaacaag agatcggaga aatgacatgg tcagatgcgg
     4501 gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt ggtcaaaagt
     4561 tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg aaaaagatca
     4621 ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat caagacgtct
     4681 tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta ttccatttat
     4741 atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca tatttatctt
     5041 cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgagacgag
     5161 tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt ttgctgcttc
     5221 acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg gaaaaagcag
     5281 attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca tatgatattt
     5341 tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat tttttacagt
     5401 ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc tttgctgtac
     5461 gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct ttgtctcctg
     5521 taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg aagacagcag
     5581 gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta gctgaacaac
     5641 aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca gatatccaac
     5701 agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat ggcgtatttg
     5761 aaactgcacg caagttgagc cgtcagcgag tgacacttcg atggtatcct gaaagtggtc
     5821 atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg ctgaaggttt
     6001 agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa aaccaatctt
     6121 gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg gagacaccgt
     6241 agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag aggggaaagt
     6301 agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg cttattcaga
     6361 agaagaaata caagaaacgg atctttacgg cgttgttatc ccgaaagata gaaagctcaa
     6421 ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa gtattgtgct
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg tcgtggctaa
     6601 tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag ttccagatca
     6661 aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca ttgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat tagataacgg
     6781 taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac aaggcagtca
     6841 attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc gagtgattcc
     6901 gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg ttccaaggct
     6961 gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc atgatatttt
     7021 cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga tttcttttga
     7201 ggatcgcgaa gcaaaaatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca atgaaacggt
     7321 agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctac ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gggaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agagggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaga aagtgcgagt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtagat tttgaacttg tttccgcaga agaggttaca gcgttggaac ggccaaaaac
     8101 acgaaatgga aaatcaggaa aacgcgaagg aaaacaagca ggaaaacgcc cagaaagaaa
     8161 tggcaagaag aacaaacgat atagcgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 gaagccattt tacaaaggtg ttaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaatt aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaaccg agatcaagtc
     8401 gatacgaaac agccggatca atctaaaaga tggatttatt cgcgtccgca acggagaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa aaaacaaatc atccggcttg aaaatgaatt
     8581 gaaaaatact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcga aagggaaaaa atcttatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cataggtttt ttcttagatc aggagcaccg tgtataaaga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgttccac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatcg gtttagtaac aaaaatcgtt ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttatttgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaacca tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg gtaataagct taggctggtt ctcattgccg ttagcaattc
     9661 cattagagat ggtctggatc gcattctcct tgtttatcgg aagcatccaa gcatttgtct
     9721 tcgttacttt atctatggtt tatatgagcc ataagattga agtggaagaa taatattcgt
     9781 tttaaaaaca aactactatt tatctaattt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agtcgaagct gttcctatct taggggtagt tgtcgccttg
    10021 attctagttt ttggtgttta attgaatcag tattaaggca gaaacaattt ggtttgttac
    10081 tgctttttta catgaaaact agaagtactt caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggaa atgtcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cattttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttggatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agtaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gatgttgcag aactatctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcacg aatcattaat caatcaatat
    10621 atcgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaataaagca gaagaaattt atcaagatct
    10741 cgtaagctta cgtcaaatct atgaacaagt acctggatta ggtaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgttagtg gctatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgcgtataac cgaatggatg atcttttgtt
    10921 catgatcgaa gaatatgaac gtcgatatga tgaacatcaa ggtttgctgt tgggaagtgt
    10981 cacaacagca gttccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatgaac tatcaatcag tcgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagctaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 tatgcgtaat caattaaata gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaatgg ctcatacgga atggcgcaaa
    11401 acttagaatc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggaaagatca tggaagttcc agttggagag gccttgattg
    11521 gacgggtagt taatccgcta ggtcagccaa ttgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagca atggcaccag gcgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac aggtctaaaa gcaatcgacg ctcttgtacc aatcggacgc ggccaacgtg
    11701 aattagttat cggtgaccgt aaaacaggga aaacttccat tgcgatcgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcgccgttgc tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga gttcatgtac aacgggaaac atgtcttgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca ggtcgtgaag
    12061 cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca gcaaaattaa
    12121 gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa atcttcttag
    12241 aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta tctgtatctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgtctag
    12361 acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac ttggatgcag
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaattgc
    12481 atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca catggattct
    12541 tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaaata
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgttgctc
    13021 agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg tttagtcggt
    13081 ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    15841 ctgcgacaag ac
//
LOCUS       DAFEPQ010000058        15511 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.060, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000058
VERSION     DAFEPQ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.060"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(330..632)
                     /locus_tag="LP543_002010"
     CDS             complement(330..632)
                     /locus_tag="LP543_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322171.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(852..1218)
                     /gene="ssrA"
                     /locus_tag="LP543_002011"
     tmRNA           complement(852..1218)
                     /gene="ssrA"
                     /locus_tag="LP543_002011"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1389..2162)
                     /locus_tag="LP543_002012"
     CDS             complement(1389..2162)
                     /locus_tag="LP543_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL2322172.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2306..2656
                     /locus_tag="LP543_002013"
     CDS             2306..2656
                     /locus_tag="LP543_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBL2322173.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2637..3353
                     /locus_tag="LP543_002014"
     CDS             2637..3353
                     /locus_tag="LP543_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2322174.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3353..4801
                     /locus_tag="LP543_002015"
     CDS             3353..4801
                     /locus_tag="LP543_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL2322175.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4871..5380)
                     /locus_tag="LP543_002016"
     CDS             complement(4871..5380)
                     /locus_tag="LP543_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322176.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5370..6095)
                     /locus_tag="LP543_002017"
     CDS             complement(5370..6095)
                     /locus_tag="LP543_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322177.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6318..8438)
                     /locus_tag="LP543_002018"
     CDS             complement(6318..8438)
                     /locus_tag="LP543_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL2322178.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8668..9846
                     /locus_tag="LP543_002019"
     CDS             8668..9846
                     /locus_tag="LP543_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBL2322179.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9862..10620
                     /gene="aroD"
                     /locus_tag="LP543_002020"
     CDS             9862..10620
                     /gene="aroD"
                     /locus_tag="LP543_002020"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBL2322180.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10643..11128
                     /gene="ybaK"
                     /locus_tag="LP543_002021"
     CDS             10643..11128
                     /gene="ybaK"
                     /locus_tag="LP543_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBL2322181.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(11199..12452)
                     /locus_tag="LP543_002022"
     CDS             complement(11199..12452)
                     /locus_tag="LP543_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL2322182.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12569..13918
                     /gene="gdhA"
                     /locus_tag="LP543_002023"
     CDS             12569..13918
                     /gene="gdhA"
                     /locus_tag="LP543_002023"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBL2322183.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            14030..15376
                     /locus_tag="LP543_002024"
     CDS             14030..15376
                     /locus_tag="LP543_002024"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBL2322184.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
BASE COUNT         5126 a         2749 c         3124 g         4512 t
ORIGIN      
        1 aaagacattt gggttatatt gtgagataat aataaagaag agtttaaagc gttccccaaa
       61 aaccacttcc ccataagtgt gttacgcttt aaactctttt atatttgaag ccattaaaaa
      121 gcataccata taactgtaaa aaataatggg aaaaagactt ataattggag tggtagttaa
      181 ttagtgactt atttttgatt ttatagcact gatactataa aatatagata tcatcatatt
      241 acacaatctt aatactaact taaaaaatat cccttttata agtatggtga taaaatccgt
      301 tccgggctac cttttttagg tagcctactt taatctttat acctttctgg atcaacgaaa
      361 gtatacttca aatagtcata acgccgatga tcgctccgtg cgtctggcac atcagtcacg
      421 atatcaaaca aaaaatacac atctttcttc attctggttt ttgttgccgg aattttaaag
      481 tagttcttat tagagtaata gagattgatt aataagctat cttcgattgc taaaaagaaa
      541 acttctgaat cccacacctt ataaaaatct ttgacaaatc tattcgaagg atcaaattta
      601 aaccataatt gtgtttttcc ttccatcaac ataatgttca cctcaaaaag agtatacgaa
      661 ctaatgttct ttttgtaaag taggaatatc attgataata tggatggaac atatatatta
      721 aaaagtgttc tttttgaatg ctattttgaa tgccattttc attcatttaa cgaaaatctg
      781 cgaaaataaa aaatgctcaa acccttataa aataaggatt taagcatcta gcgaaatctg
      841 ctgaaatctc acaatggaga cggcgggagt cgaacccgcg tccaaacaca tcgccactta
      901 aaaatctacg ttcatagtca actcatttaa ggattcactt tacagcttgc cgagtgacag
      961 gcattctgta tcgctagtct gatgttctct ttttcaactt acagacggaa agttgaaacg
     1021 tatcccacta aagttaggac ccttactcga gcacatgggc gatgccgaga ggatctacgc
     1081 taactgtttt taggcagcta aagcgtaaga gttgttttcg tttttagcag ttatatttaa
     1141 ctgtaacgtt tttacgtaga cgtaacctac gaaacgcaat tcaagctcga ctgtgcctgt
     1201 cgaatccgta acgtccccgt tagctaacaa gggttacgtg tatttttcat acaaaattag
     1261 tatagcatat attgggataa attcaataaa aaagcgaatc atatcccatt atttatataa
     1321 taactgcccc tttcttaaga aaaaataagc gattttctta agaaaagagg tagcttcaaa
     1381 aaaagatctt aaatcagatc aaaatacttc aatgcgtgga caattccgcc ttctgtattt
     1441 ttctttgtga taaaggtaga aagttctttt aatccgtcat aggcgttgcc catagcgatt
     1501 ttgttgtcgc aagcttccaa caaggcaaaa tcattcggac catcgccaaa tccaaaagta
     1561 ggaacttttt ccagctgaag ttctttttgc agacgtttga cacctgcccc ttttgagatt
     1621 cctttcttca ctgtatcgat cgagtaagga ccattccgat aaaaagttag ctcaggaaaa
     1681 tgttcgtgat agtattcgtc acctgattga gataagatca gcaacatatt tactgtgttt
     1741 tctttaaagc ctaaaggatc aatttccgga acatctgaat gaatgaagtc gtaagcattg
     1801 atcattgttt ggctatgacc ggtacaccag attttctgat cgttatagta agaaagttca
     1861 tgaccgttca actgtacatg atcatacatt ttttgacatt cttctgaaga aaatgtttga
     1921 gaatagattt cttcgccgtc tactcgaatg aaagaaccat tcatggtgac cgcagaagta
     1981 atgcccgcat cttccatgat agagtggatt tctgcttctg tacgtcctgt agcgatgacg
     2041 gggagaacat tgttttcttt taatgcgttc atagcttttg ccacttcggg tgtgattttt
     2101 gatttcgcat ctagtaatgt tccatcaaga tcaaagaaag tgattgcacg ataatttgac
     2161 acaaataaaa ctcctttttt attggaataa gtttatctta acaaagaatg aacaaaagac
     2221 aaacctttcg tgaagaattc ctgaacatat tggttcagtc aacaatttag agtatactat
     2281 aagaagagaa tttaggaggg aaagaatgat ttttttcagt attggtatct tattcttaat
     2341 aattggcagt ctttttcgga ttctgccctc aaaaggaaat ctgccttttt atgggtatca
     2401 ttcgcctctt gccgcaaaaa cagacgctca ttggcggctg gctcaaaaga caagcggaaa
     2461 ctggtttttt cttatgggct tactgatggc attgatcggc tattatttaa agacatctgg
     2521 gcatacgaat tattttctga ttgaaatgct tctgctagta tttccaatta tgcctatttt
     2581 tattatgaca gaaaagaaat tacagaaata tgacctggaa actggaggga acgataatga
     2641 atattttaat gattgaagat aatcaatctg tttctgaaat gatgcagatg tttttcttaa
     2701 atgaaggttg ggaagcaact ttcaagtatg atggaaaaga agggctcgat gcctttttag
     2761 aaaacccaca acattgggat atgattacac tagacctgaa tttgcccaca ctggacggta
     2821 tgctagtagg aagagagatc cgcaaagttt caagtactgt gccgattatc atgctgacag
     2881 ctagagattc tgaaagcgat caggtgatcg gcttagagat gggggcagat gattatgtca
     2941 caaaaccttt cagtcctctg acgcttattg ctcgaatgaa agctttgcat cgccgtagtg
     3001 agttggcaga agcaagagaa gaagcagtag tttcagatga tcattttgat atcgagacta
     3061 gtcatttcaa gatgaatacg aaaacacgag aagcgtatct ggacaaccgg ccaattgaag
     3121 gactgacgcc taaagagttc gatctattgt ttactttagc aaaaaaacca cgtcaagtat
     3181 tctctcggga gcagttgctt gaattagtct gggattatca gtactttggt gatgaacgga
     3241 cagtggacgc ccatatcaag aaattacgac aaaaaatcga aaaggtcgga ccacaagtca
     3301 tccaaactgt ttggggagtc ggctacaaat tcgatgattc tggtgttgcc taatgcgtta
     3361 tttgtatcag caactcttag ctttctgcgg aatggtcgcg ttgatcttat tgatcgttgg
     3421 agtttctttt acacaactga caaaacaaac gattgaagaa aacaattacc agcaactgtt
     3481 tggatatgct gaatcagttg aaaagacaac gcaaacctat gccgatacgc tcccgcagtt
     3541 tagtcaagat caggcgtttc aaaatgcatt gctgctgaca gagcaagtac tgacagaaca
     3601 aaatgtgaac tttatattta tcgataaaaa cgaacgtgtc atttatccga ctaatacagc
     3661 tttactgaat tttaccttaa cagctgatca atggaaaaat ctgaggaatg gacgtcaaga
     3721 aaaattcaca tcgaataaaa atattttagg acaagcacag gcgacctctt acgcacttgt
     3781 gccatttaat ttgaatcatg aattctatgg agcacttgta gttagtcaac ctgcgcgaaa
     3841 tatcgataac agcgttcgtc caattacact gaacctgttc aaaggattta ttttttccag
     3901 tatcgttgca gttattgcca gctatgcttt tgcagcattc caagttaagc gtatcaatcg
     3961 gttaaggaat gcgacgaaag aagtgacgaa tggtaatttt gatgtacagc tccccgtaca
     4021 tgacaaagat gaatttgatg agttagcaga tgatttcaat aaaatgacga attcattgaa
     4081 agaatctcaa gcagaaattg aagaacaaga aaatcgtcga agacaattta tggcagatgc
     4141 ttctcacgag atgagaacac ctcttacgac aatcaacgga cttctggaag ggttagaata
     4201 caatgcaatc cctgaaaatc agcgagaaaa cgcgattaaa ttgatgaaaa atgaaacaga
     4261 gcgtctgatc cgtttggtga atgaaaactt ggattatgaa aaaatccgta cgaatcaaat
     4321 cagtatggtc attaaaaaat tcaatggaac agaaacattg aaaaatattg ttgcacagtt
     4381 agaagctaaa gcagaagcag caggagatac attgacattg aaagccgttg atgatattga
     4441 tgtttacgct gattatgacc gatttgtcca aatcatggtc aatattattc aaaacgcgat
     4501 acagtttact gaaaacggcc aaatcacgat tactttagaa aaaggctatc ttgaaacgat
     4561 catcacgatt gaagatacgg gaattggaat gtctgaacaa caaatgaaaa gtatctggga
     4621 tcgttattat aaagtagatc cttcaagaaa aaatacaaaa tatggtgaat caggattagg
     4681 tttaccaatc gtgcagcaac ttgtacgctt gcataaagga aaattagaag tagaaagcga
     4741 actaggaaaa ggaacaaaat tcgctgttcg tttgcctgac gtagaaatca aaaaagagta
     4801 aaaaacaagc ggtataaaag atcatgaatc gatcttctat accgcttgtt tttactataa
     4861 aatgattgat ttagcccgcg ggctctaaac gatcaatata aggactttga gcaatagtag
     4921 catcatgagt aaagagtaag ataccttgat tttcttcatg agctaaataa gaaagcaagt
     4981 ggagtagctc ttggatctcc aatacactaa gttcatcaaa tgtatcttca ataacaatat
     5041 tgtttcgctt ggaaagcaag gcatgaagta attgcagctt tactttttca aaagacgtca
     5101 actcagctgc cgcacaatta agcaaggaag gagagatatc aagtttttct tgataggcca
     5161 aacgatcttg tttgatttct ttttccggta catccaacag gacatggtct ttaagattca
     5221 gataaggaaa catcggccaa tttttttgga tgatcgcaaa attatccagt tttgcaacaa
     5281 gaacctcttt tttcaagtgc tccaatattt tatgctgttt ttcgagagga cacaaaataa
     5341 cggtcaagtg ttcagcatga atcaaatatt caggtttcat tacgtccagg cctcctactt
     5401 tgcaaatgat ggataaaaaa agtgaaaggg aatagactaa gaatgagaca gcatccactg
     5461 agcccaataa gagtttgggc aaatccctta agtatatctg tgaaaaaaag atcattttgg
     5521 aaatcgatgg taaaaaaagt ctgttgatcc atcggaattg taagtccgct taccgaagtc
     5581 aagatatcac ggatatctgg cagttcagta gtctttaaga caagaaaatt gatcctttga
     5641 agaagcgctt cataaactga ctggaagctt acgatccaca ataaaaccaa gatacaacaa
     5701 agaaagagag gaaagaacag ctgccagaaa ataaaaagga ataatttggt tttagaaaga
     5761 ccagtcaggt gccaattgat caattcctgc ttgttttgcc tgactgaata gtaaaagaaa
     5821 aagataaaaa ggaaggcaaa tagaaaaaat aaactgaata tgccttttat ataaaatcgg
     5881 ctagctaatt ctaaagccgg aagaagctct gtagatgcag aagcgccaat aaatgaaagc
     5941 cgatcttgga cttgattggc taaagccgat tgcaaatggt aaagagtcag gcatgctatc
     6001 aataaaaaag cagaaaagct tgaaaataaa caatagagta ttgtctgctt tttgtaataa
     6061 attaatcgag cagtagcata gcggattatt ttcatacttt tcacctctat aaaaagtata
     6121 gaaaatgaat atgaataaag tatgaaattc ctttagtgaa atcacgaatt aaaaccagaa
     6181 aatattttgt aaaaagcaag aggttgtgaa gaagctgccc tattctatta ccgtagatgc
     6241 aagcttttga acactgttta ttcgaaaaga ggagcagcga caataatttt gtcgctgctc
     6301 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
     6361 gtttgcgttt tataaagatc aacagttgat tgtccgttgt gttcactaaa gacactagtt
     6421 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
     6481 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
     6541 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
     6601 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
     6661 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
     6721 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
     6781 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
     6841 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
     6901 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
     6961 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
     7021 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
     7081 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
     7141 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
     7201 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     7261 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     7321 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     7381 ctaatgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     7441 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     7501 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     7561 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt catgctggag accattctca
     7621 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     7681 ttttttcctc tagcgagccc gaacaaatcg tcatttggtg ctgcataatg ttcttttaca
     7741 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     7801 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     7861 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     7921 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     7981 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     8041 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     8101 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     8161 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     8221 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     8281 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     8341 aaataggcaa aaatcgtttt caaccaaagt aaaacgacta gtaaagaaaa aaatccgagc
     8401 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     8461 cttaatcaca gaagtgtcct gaaaccatgt tacacagctc acttattctc atttatttta
     8521 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     8581 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     8641 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     8701 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     8761 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     8821 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     8881 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     8941 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     9001 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     9061 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcatagg aggctatgag
     9121 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     9181 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     9241 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     9301 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     9361 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     9421 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     9481 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     9541 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     9601 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     9661 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     9721 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     9781 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     9841 cattagaagg agcgatgcag aatggttcgg gtaaagggat tgaatatagg aagcggtaga
     9901 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     9961 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
    10021 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
    10081 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
    10141 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
    10201 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
    10261 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
    10321 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
    10381 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
    10441 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggttggc
    10501 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
    10561 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
    10621 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
    10681 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
    10741 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcgta tatttaaaac
    10801 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
    10861 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
    10921 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
    10981 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
    11041 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
    11101 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
    11161 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
    11221 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
    11281 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
    11341 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
    11401 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
    11461 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
    11521 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
    11581 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
    11641 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
    11701 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
    11761 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
    11821 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
    11881 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
    11941 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
    12001 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
    12061 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
    12121 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
    12181 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
    12241 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
    12301 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
    12361 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
    12421 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
    12481 tctgaagaaa gaaaacagaa aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
    12541 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
    12601 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
    12661 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
    12721 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
    12781 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
    12841 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
    12901 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
    12961 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
    13021 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
    13081 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
    13141 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
    13201 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
    13261 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
    13321 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
    13381 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
    13441 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
    13501 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
    13561 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
    13621 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
    13681 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgtccagcg
    13741 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
    13801 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
    13861 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
    13921 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
    13981 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
    14041 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
    14101 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
    14161 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
    14221 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
    14281 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
    14341 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
    14401 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
    14461 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
    14521 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
    14581 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
    14641 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
    14701 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
    14761 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
    14821 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
    14881 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
    14941 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
    15001 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
    15061 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
    15121 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
    15181 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
    15241 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
    15301 caagaaaaac atgttcgcac ttctaggcaa accaggcttt gaagatcttg ctaaagaatt
    15361 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
    15421 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
    15481 ttcagaataa ggggctgtga caagacgttt g
//
LOCUS       DAFEPQ010000059        15505 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.061, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000059
VERSION     DAFEPQ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.061"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(87..500)
                     /locus_tag="LP543_002025"
     CDS             complement(87..500)
                     /locus_tag="LP543_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBL2322185.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(576..872)
                     /locus_tag="LP543_002026"
     CDS             complement(576..872)
                     /locus_tag="LP543_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322186.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMECDKWLAKSKDNLAVVRLVDIELINDK"
     gene            complement(872..1078)
                     /locus_tag="LP543_002027"
     CDS             complement(872..1078)
                     /locus_tag="LP543_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322187.1"
                     /translation="MKIYVVKFGNQFYRSDERSIGANTLSIVDILQSARWFDNLEEAN
                     QVSRRLGGLAQVYELVTIDHEEVE"
     gene            complement(1075..1272)
                     /locus_tag="LP543_002028"
     CDS             complement(1075..1272)
                     /locus_tag="LP543_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBL2322188.1"
                     /translation="MNNRHRRVTKLRKQELNALKAKFEKEYGISIEEAYKVASQCVAD
                     ASEAIRKFGISILDIKWEDTE"
     gene            complement(1279..1509)
                     /locus_tag="LP543_002029"
     CDS             complement(1279..1509)
                     /locus_tag="LP543_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322189.1"
                     /translation="MKYEIALSEADVQSIINGRKVKFKLSDGTEIVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            complement(1532..1837)
                     /locus_tag="LP543_002030"
     CDS             complement(1532..1837)
                     /locus_tag="LP543_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322190.1"
                     /translation="MNVQVYLKRDLARFPRSWDEYSCPFEAEEESYLMSANMIEITRD
                     DADEVDKKRSWYSVSNPMYTAVIEDYHISDRFILVDLERPKKRIKQYRRCGFSTKKW"
     gene            complement(1834..2031)
                     /locus_tag="LP543_002031"
     CDS             complement(1834..2031)
                     /locus_tag="LP543_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322191.1"
                     /translation="MKAYTIKFYGNKTFVYILSGLRLDFRGYSKEDLLESLYGYGYLN
                     EDELISLEKFTNAWTIEEEAE"
     gene            complement(2034..2661)
                     /locus_tag="LP543_002032"
                     /pseudo
     CDS             complement(2034..2661)
                     /locus_tag="LP543_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337468.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
     gene            complement(2654..2974)
                     /locus_tag="LP543_002033"
     CDS             complement(2654..2974)
                     /locus_tag="LP543_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017547333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HBL2322192.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFEQMRELDKQTNWRKKLHQDRFKFVEKYKEILDEYLE
                     DSNE"
     gene            complement(2974..3279)
                     /locus_tag="LP543_002034"
     CDS             complement(2974..3279)
                     /locus_tag="LP543_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322193.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            complement(3276..3437)
                     /locus_tag="LP543_002035"
     CDS             complement(3276..3437)
                     /locus_tag="LP543_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBL2322194.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(3434..3751)
                     /locus_tag="LP543_002036"
     CDS             complement(3434..3751)
                     /locus_tag="LP543_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HBL2322195.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            complement(3998..6310)
                     /locus_tag="LP543_002037"
     CDS             complement(3998..6310)
                     /locus_tag="LP543_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBL2322196.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTGEQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            complement(6325..6852)
                     /locus_tag="LP543_002038"
     CDS             complement(6325..6852)
                     /locus_tag="LP543_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HBL2322197.1"
                     /translation="MKIKRRMINMTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATK
                     SGAEFIDIDLIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSY
                     NGFDELLADFVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGP
                     ERSRATTVRNDNLPF"
     gene            complement(6827..7300)
                     /locus_tag="LP543_002039"
     CDS             complement(6827..7300)
                     /locus_tag="LP543_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL2322198.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            complement(7291..8640)
                     /locus_tag="LP543_002040"
     CDS             complement(7291..8640)
                     /locus_tag="LP543_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL2322199.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            complement(8615..9301)
                     /locus_tag="LP543_002041"
     CDS             complement(8615..9301)
                     /locus_tag="LP543_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL2322200.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILYGIVRLPE"
     gene            complement(9401..9832)
                     /locus_tag="LP543_002042"
     CDS             complement(9401..9832)
                     /locus_tag="LP543_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322201.1"
                     /translation="MKNKLAKTTVIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREIEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            complement(10041..10217)
                     /locus_tag="LP543_002043"
     CDS             complement(10041..10217)
                     /locus_tag="LP543_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322202.1"
                     /translation="MEHENVKDALKAAIEIAESKGIKVDGKPATTEDVQEMVRELLYN
                     VADLLGMSEVYLGK"
     gene            complement(10234..10713)
                     /locus_tag="LP543_002044"
     CDS             complement(10234..10713)
                     /locus_tag="LP543_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HBL2322203.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            complement(10646..10963)
                     /locus_tag="LP543_002045"
     CDS             complement(10646..10963)
                     /locus_tag="LP543_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322204.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            11131..11589
                     /locus_tag="LP543_002046"
     CDS             11131..11589
                     /locus_tag="LP543_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322205.1"
                     /translation="MIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFLVKH
                     DRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLLSPI
                     PLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            complement(11680..11862)
                     /locus_tag="LP543_002047"
     CDS             complement(11680..11862)
                     /locus_tag="LP543_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322206.1"
                     /translation="MDKLNTTIVFSAPIIIYLLSVWGNIPALIGTIVYMVWIFIGLDE
                     AEYRAKKASREGLTNE"
     gene            complement(11911..12171)
                     /locus_tag="LP543_002048"
     CDS             complement(11911..12171)
                     /locus_tag="LP543_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL2322207.1"
                     /translation="MEVILTPENEAALRSYIHEIITDEIAKARRDASVDKRVLKQIEI
                     AKYFGVSTATIRKWEDKGLPFGRIGDQKFYDKEKCRAWVLAQ"
     gene            complement(12185..12376)
                     /locus_tag="LP543_002049"
     CDS             complement(12185..12376)
                     /locus_tag="LP543_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322208.1"
                     /translation="MSNIDNGREAIKEFMKANNISEYDLATAYGRSRTWIQRVLSGKD
                     KGPAVNAFILEVIRDHKIR"
     gene            12668..12991
                     /locus_tag="LP543_002050"
     CDS             12668..12991
                     /locus_tag="LP543_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2322209.1"
                     /translation="MTVFERIKFLAKKHSKTMKQVTIDLGYSENYFYSLKSGKQPSAE
                     KLTEIANYFNVSVDYLLGREKKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKA
                     LEENE"
     gene            13009..13431
                     /locus_tag="LP543_002051"
     CDS             13009..13431
                     /locus_tag="LP543_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBL2322210.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSALYDNSTTFHSKFETEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            13513..14148
                     /locus_tag="LP543_002052"
     CDS             13513..14148
                     /locus_tag="LP543_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBL2322211.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            14257..15405
                     /locus_tag="LP543_002053"
     CDS             14257..15405
                     /locus_tag="LP543_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL2322212.1"
                     /translation="MATFEQYKKKNGEKLWKFQTYLGVDPLTGKQVRTTRRGFKTKKE
                     AQLALTKLQLEYESNGLNKSKELTFQEVYDLWIVNYEQTVKESSFVKTKEQFANHILP
                     AFGALKINKISIDIAQKFANEKVKKFVLYREFINNASRICDYAIKLGYLQDNPFKKIT
                     VPKRKVSVHEEDTLNFFNKEELEIFLKSVEKKKDIRMYSFFRTLAFTGMRVGELLALT
                     WKDIDFNDNYIKINKTLARGKNRRLYVEQPKTKNSKRDIPVDDETMNILKKWRLEQRK
                     WLLTLGINTLSKNQLVFSNQKNEYLQLSKPRKWLEVIIKQNNLKRITIHGLRHTHASL
                     LLEAGANIKDVQERLGHSSIQITMDLYIHITDKRKEKTAAQFAKYIGI"
BASE COUNT         4356 a         3245 c         2531 g         5373 t
ORIGIN      
        1 agacctatca ctaatctttc gacactacca taataacact ggtaaatgtc taaaaaccgc
       61 catcattccg ccaaaaaacc gccaaattat ttataagcaa ttatttttcc gtgtttatat
      121 gcttctgcaa actctattag agcttccgac ttcatccgtt gtatacttct ttctgaataa
      181 cccacttcac ggctaattct gtagtttgag aagctatctg gcacacagaa gctgtagtag
      241 agtatctggc gactaattag actaagagcc atcaaagccg ctagaatcgc atctctctcc
      301 gcttctatat ccatcatctg aatgatcgcg tcttctgcct tattgccgtg ctttggtgcc
      361 ttcggcatat ccgtaataat cggcgactta atatctatca aagagcgacc tgccatccgc
      421 tccaagcgcc gaaagttctt cagcacatct ctcgcattac atcttgtctg tttgaaatct
      481 acctctcgta acaattgcat caagtcaaac cgctccttta tgtgatataa taaacttgtg
      541 gaatttatta gatcagtcgg agcgatccgg cttttttatt tgtcattgat tagttcaata
      601 tccaccaatc tcaccactgc taaattatct ttgcttttcg ctaaccactt gtcgcattcc
      661 atcgtgtttt caatgcgaat gatcgctgag tgattataga gatgctctac atatccacga
      721 aacggataga tgaacccttc tgcttcgcag cgaaccatgt caccgacttt gaattttggt
      781 ttcttacgtg ttttagggtt ctttgtcggc atatctagca ttaaacctcc gataccgtga
      841 ctgctagagt agaatccgtc ttttagtttc attactctac ctcctcgtga tcgatagtga
      901 ccagttcata tacttgcgct aatccaccaa gtcgtcgtga aacttggtta gcttcttcga
      961 gattatcaaa ccatcttgca ctttggagta tgtctacaat ggataatgtg ttagccccta
     1021 tagaacgttc atcacttctg taaaattgat ttccaaactt tactacataa atcttcattc
     1081 tgtatcctcc catttaatat ctagtatcga aattccaaac ttacgaatag cctcactcgc
     1141 atcagcaaca cactgacttg ccactttgta tgcttcttct atagaaattc cgtattcttt
     1201 ttcaaacttc gccttcagtg cattaagttc ttgttttctt agttttgtta ctctgcggtg
     1261 tctgttgttc attgtcaatc aactccctaa tctgaaagtg tcgtttatac ttgatcgaaa
     1321 ttctttcaag tggttctcta ccacagaatc agtcacgtta aaacgatcaa ttaatacagg
     1381 agctgccata tctttcaaat aactttgtct gataactatt tcagtaccat cagaaagttt
     1441 gaatttaact ttgcgaccat taataattga ctgaacgtct gcttcgctta gtgctatttc
     1501 gtatttcatt ctgcttcctc ctgttttatt ttcaccattt ctttgttgaa aacccgcaac
     1561 gtctatactg tttaattcgt ttcttaggtc tttctagatc aaccagaata aatctgtcgg
     1621 atatatgata atcttctatt acagccgtat acattggatt cgaaaccgag taccatgatc
     1681 tttttttatc aacctcatcc gcatcatctc ttgtaatttc tatcatgtta gcagacatta
     1741 gatagctctc ttcttctgcc tcgaatgggc aggaatattc atcccagctt cgaggaaacc
     1801 tggccaaatc tctttttaaa taaacttgaa cattcattcc gcttcctcct caatcgtcca
     1861 agcatttgta aatttctcta agcttattag ttcatcttca tttaaatatc cgtaaccata
     1921 taaggattct aaaaggtctt cttttgaata gcctctaaaa tcaagtctga gaccgcttag
     1981 gatgtataca aaggttttat ttccataaaa cttgattgtg tatgctttca tttctattcc
     2041 tcttgttcta atccttctat cactggcaca gcaaatggcc aatatctttc atcaattgct
     2101 tttatttctg attccgtaaa tttcatatca tcaaaatcat tgttgtctgc aattgttata
     2161 tttcctcgat catctttcat tagatacgtc tctaaatcca aatgattaaa atacacaact
     2221 ggtaacttca cataatacaa cggttctttc tcaacctcgt aaccatacag caacgcattt
     2281 attgcttttt ctttattaag ataaaggcta tcttcaacaa gccaatctag taactctctt
     2341 gttgtggaag aattttcgct tttaccaagt tcatagtcca cgccataacc ccaacctgca
     2401 cgtgcaataa gagaaatggc ataaaacttg tcacgttctg ctataactct ttttgcccaa
     2461 tcatcaaaaa aacttaggca taactggttt ctgcgattcg tctagtttta acaccgtatt
     2521 aaccatatga acgacttgag aataagccat caccatgccc tcatagtaat ttttgctttc
     2581 tggatgcgtc agggctttgt gctgtttttc tagtgaaatt ctctttagtt cattaatcaa
     2641 ttcctgttta tttttattca ttgctgtcct ccaagtattc gtctaatatc tctttatatt
     2701 tctctacaaa tttgaaacga tcttgatgaa gtttcttcct ccaattcgtt tgtttatcaa
     2761 gctcgcgcat ctgctcaaag cctttttgaa tttcgttgta ataaaattca atgtttgctg
     2821 ctgctttcca atgcctgcta cttcgcactc ctgctcctgt ttcagccatt tctaacttaa
     2881 ctaattcagc tcgttctttt gattttttgt ctttctgaat cttcatcatg attttcttga
     2941 ggatgatgtc actgtattgt gtaatgagat ccattatctc tcctccacat acctaaactg
     3001 tcgtcctttt gaatcaatcc ataagctcct agctctatcc cagataatgt ttttgcttaa
     3061 tccagtaatt tcagataact gttcagctgt acctgttact agaattcgat caccatgcca
     3121 gattgcaatt tttctcggcg ttttccgttt aggcttttca gtccacattg atttaccgag
     3181 cttttggact tctgcaacta tttctttgtc ttcctgccaa gattctgact tggttaattc
     3241 agcaattcgt ttcattgtcg ctttcttatc cacgctcatt cctccaatct acgaatttcc
     3301 cttcttaagt tctctatgtg caaatcgatt gcctttctcg ccgtttcatt gaccatcact
     3361 gcctttgttc gttctagatc gtcaatttca cgctgaatgt ttcgaatacg catttgaatc
     3421 acttcttctg ttgtcatgat gattcctcca caattttcaa tgcttcttcc actgatcgtg
     3481 caacaccata tagtacaggt tgaccgttta aaaattcagc aaaccttttt tgatcttcac
     3541 gcaacttacc gcgttcattt tttacttcta tataaattgc tttcccatca gaattacgat
     3601 gtccacatag atctgggaac ccttttggca tcccgtaaaa ttgccgccct tcgatcgttg
     3661 tcacttgtcc cgcattggat cgaaaaatcg tgcaaccatg ttttgataag gcaattctga
     3721 tctcgtcttg tatttgatgt tctgatttca atgtaaccct ccagactgtt taatcgttgg
     3781 tatcaaagca tttaggaaga ttttggtgga gagtttggag ggtcagaccc aaaaacctat
     3841 tcttttttat ataatttact ttttattttt tactttaata ctttattatt tttttaaaac
     3901 cctccagaaa gaaagaaaaa tataatataa acactgatat atcaacgttt ctaagggttg
     3961 gagggtttcg ttctcaaccc tccagaaccc tccagattta tttaaaaagt tctctaacgt
     4021 tatcgtattt tcttggtttt aatgtgattc ctttataaac cataaatcca ccagcttttt
     4081 ctttgctata tttctttgaa atctctctgc caaacttcgt gttagtgaaa gtatgctcgc
     4141 cattatctga tgcccattca cgataaactt tgtataattc tccgcctgat atttttagtc
     4201 ctggacctgt ttcgcaacac gtttcgataa atgtacctat ttgatccatt tcgtttcgat
     4261 aatcctgact cgctgcaaca accgactttg gtttctccaa gccctctctc tgccacaaca
     4321 ggcaaccatc gattgcccat ttaaggatcc cttgcaactc cgtctgcaat ttaaatttca
     4381 gatttttatc ctttttgtga tccgggattt ggacagtgaa tggaattaaa tttaaccgtc
     4441 gccagatgcc gtcatcggtc ccacggatga tgggcttgtg gttcgttgcc agccacaact
     4501 tgaattcagg ttcgaactcg aattcttttc cataaaggtg ccgagccgtg actttatcac
     4561 caccagtcaa ttgtttgacc agaccttcgt ccagtcggac gccttcattc ggttctgacg
     4621 aagtgaccaa ccgcgcacct ttcaatcgtg caatatcact gtttgctgat gattggcttt
     4681 gcttgaccat gatcgtttgt gcctgcatag tcatcgcata gtttcccatc aattcagaaa
     4741 taatttccaa aaataccgac tttccatttg acccatgacc gtagaggatg aacatacatt
     4801 gctctccggt agatccggtc agggaatatc cgacacattt ctggatatat ctgattaatt
     4861 cttgatcatt gtcaaaaatc tgattaataa attcttccca cataggacaa tcgaccgatt
     4921 ctgtgtactc gacatttgaa acacgtgtaa acattttgtc tatctcatgc tcgtgtagaa
     4981 gaccgtcatg caaagacaga tagccgcttt gtgtgttgaa cagcgtttta tctttgtcga
     5041 actcttccgg caatacggat agtcgatgtt tcaattcgtc catcatagcg ttttttccgg
     5101 cattgctgcg agaacgcttg acgtgttttt ctttcgcttt catcaaagct tctttggttt
     5161 catcgtctgc gccttcaggg atcgttaacg gttcttttcc catgatgtct actgtcgtat
     5221 cgatcatttt ccgaacttcg ccggtattgt cctgtagcca aactttccca tcgtagtaat
     5281 accaagattt atcaatgtac gaatatttga ctaacgttcc gtagatatca gtaaaccgat
     5341 ccgcgttgcc ggtgtcgtcg tacgaatagt atttttcctc ttttggctgt tcctggtctt
     5401 tgataaaaat cttaaaatca gatttccgtt tggggttgta gacggaattt gtatcagcaa
     5461 ttgccttatt tagtagagat tctccgtaag ttgccgatcc ccttttttgg tcatatttgc
     5521 tacggatcat tgcagacgct ctgaaaatct catccatttt ttcaaagtct ctaccggtcc
     5581 aaaaagccaa gtcgttggcg aacgccatat ccgcttcgga ttgcgaggaa taaaaagctt
     5641 cccaaccgcc gttcataaag actttgaatc ttgccccttg ggaagacgat tcagcacgtt
     5701 tgataatttc ttcaaccggt aaatcaacgg tattcatcag cggattttct tctttgaatt
     5761 ggatgatttt ttcatctcca acgtagcgat catataggcg tttgatatcc tttggattcg
     5821 gctcagttat ttctaaatat tttgatgcgg catttccagt cataacgaag aatcggccgt
     5881 tctggtacat ttctacgtca cctttcctgc gtcttccgcc gggcaattct ccgcgagcaa
     5941 taatgtggat gccagtacct gattgagaat actcagcata ggatctcatg ctttcgataa
     6001 attcatagat aatattttct tctatatcac ctgatttaaa tcgctcaact tcaccttcag
     6061 cattatcgat atcaatgccg aaataaggtg gtttgaaaaa gaaccccagt ccactcattc
     6121 cgtagtgatc aatagcagcg agagcggtct gaaaatcaga ccaggtactc tcgtcattgg
     6181 atttggcttt atgcccgttg ttggcgttat aagggatctt cgtgtgcttc tgccgctttt
     6241 catcccaaac tagcttataa acacaccatt gtttgagatt tttaagctct tttggaattt
     6301 tttcatacat tgattacacc tcaattaaaa tggaagattg tcatttctaa ccgttgtcgc
     6361 tcttgaacgt tccggtccga aggatggttc atcaccattt ttgaattggt gattcattac
     6421 gccccgtgtg ttcgttttat cccaagactt cacattgacg tttttatatc catttgactc
     6481 ttcagttttc acgcgtactg aggcggtttt caaaacaaag tccgccagca attcatcaaa
     6541 cccgttatat gattttccgt cttccaactg tagcgcttgt gcaattgcca tgatcatacc
     6601 ttcgttatat ttgccggtag ctttggcttt ccagattttt gcgaaaatgt gtttattttg
     6661 gaacggttga tccacatcgt tacgaacgat caaatcgata tcgataaatt ctgcgcctga
     6721 tttagttgca ttttctacag ctttgtttac gaccacttcg tacgtaccgt ctttgatttc
     6781 gttcccttgt tgcgcttttg aaaagtctaa gttaaatcct gtcatgttaa tcattctcct
     6841 ttttattttc atatttttct aagataggtt tgaaatattt ttcttctgct gcaagtcgtg
     6901 cttggattgc ttcttctttg gtgttgaaat ccttttgtaa gacatattct cctttgaaat
     6961 ataatcgtgc tttccattta tttcttcgtt tatcccaatt aacgcctttt gttccaccga
     7021 cagacaaagc cttaacgtaa gttccttcaa catggtattt ttcattagct ttttttaaat
     7081 taacgtagcc actctcttcg cctgtcccgt taacaacacc tctttttata ttagatagag
     7141 aagattcaaa aacttcgtta tttttatttc tagcgattac aatttgattg cctgaactat
     7201 ctctttttcc agtatcacct attattgtta cgccatttat tattgaacct tgaacatctt
     7261 ctgctttttt accaaaaata ccgcccattc ctacaaccac cccttcctct tagcagccat
     7321 ataagcccaa ccaggtttaa atcctttagc tcttgctatg gcatataagt cctctatact
     7381 ttctgcttca tcttccgtca tgttgtaata tttattgttc tcaaaattca aagtaatttt
     7441 cgtttctccg actttcatta attcagccga ttcatcgatt tctaactctg actttcgttc
     7501 ttcaatcggc tgcaactctc cgcaatacgg acaaatatta tcgccttttg gccgttcgta
     7561 agcaccaaaa caaaacgcgc attgaacgat agataaatca ctatcagttt ttgttttctt
     7621 ttttgtatct aaactccatt cacgatccat atcaggcaat ccaaaacggt tcacattacc
     7681 cacatggtca atgatgattg acgttttatc tggtcggtaa cgcatccccc tcatcgattg
     7741 ctgaatgtac aatgataacg attgagtagg tctcaacata attacagtcg aacaatcagg
     7801 aacatcaaac ccttctccga tcaaatcaac attgcatagg attttaattt catgatttct
     7861 gaacgcttcg ataatatctg cacgttcatc ttttggagtt tttgcatcga tatgtgttgc
     7921 tttataacca gcatcgttga atatttcagc agtgtgttta cttgcatcga tactgtgaca
     7981 gtatgctatt gcctgttcac cttctgctaa cgttttataa tgtttcaaca cgtcgccata
     8041 aatagtattc ttcactgcct tatccattga tttctttgta aaatctccag tcgaagcttt
     8101 ctttaattcg gctgtatcga tcaatttagg tgcataatat tcataaggag ctaatctatg
     8161 attctcaatc aaccatttag ccgatactcc ttcaattagc aagtcgttca catcccccaa
     8221 cccactaccg tttaaccgga tgggtgtcgc ggtaaaacct aatcgcggaa cgtccgaata
     8281 atactcatag atttttctgt aggacgctgc taacccgtga tggttttcgt cagtgatgat
     8341 cagttgtggt ttcttgatgg ttttaagatg tcgtacgact gtttgaacca tgccgaaatt
     8401 tacaagctga ttatctacgc ccatcttttg aaacgtatct ttgatctgat caatcaattc
     8461 acgacgatgg accaagaaca acactctgtt gccttttcgt gtggttcgtt tagcgatctc
     8521 tgcaaccatg actgattttc cagaaccgca cggtgatacg atacatggtg ccttgtaacc
     8581 atcaacatag gcttgtcttg cacgatcgac taaatcattc tggtagtcgt acaattccat
     8641 ataaaatcag ctcactttgc ttacagccag atctattatc caattgattt ttggcatagg
     8701 ttgaatctgt ggcttgtaaa ataaatccgc gttcatcgtc ttcctttacc atcaatcgac
     8761 caacaacgtc acataatccc aaaatattgt ttcgaatctt tcgattgatc tgcggcaaag
     8821 cgatgttgta agaagatcca tccgaatccg tccacaaatc gatttcttcc catgcggtcc
     8881 agataaggtt gctgtctaaa tttttcatat agcgtaggct gttcaccatt ctaaattgca
     8941 tatattgata atcgccttgc gaaggaacgc cgttgttttt tccttctgcg cctaaactgg
     9001 acagaataca ccgttccaat tcgctgacat tatcgacaac cacgttgtca taaacaccta
     9061 cataattggt agttaaatca gccaacgtgt tgccccagtc gttccaggta tcttcgttgt
     9121 cgatatatac gatatcgaca tttttctcac ctctaaggac ttttgaagtc cgatcaatat
     9181 caaggatcag tgttttccct ggaaaatatt tagccgttga agttttacct tttcctggag
     9241 caccgtagat caaatatgtt cccttggttt gttcgatatc cgtcgccttc ttaatctgca
     9301 tcaaaattct cctttctatt ggttaaaaaa gtttttcgat tgtcggtatg ctgcattgtc
     9361 gatccactat cttcttcaac tgatatagtg attcttttaa tcatttaccc aactccccta
     9421 ctttttgatc tgtatactgc cgtaattcgc tcacacgctg ttctaactgc tccttgtcgt
     9481 tttgcactgt ggacaattgt tggcgcaagc tatcggcttc ctgttgcttt gtagcgatct
     9541 cctgttgctt ttgctcgatt tctcgttgct tggcttcaat ttccttttgc ttgtccgatt
     9601 tgatttgctc gatttctgct ttcagctgct cctgtgtgcg agtgttgttg gatagctgtg
     9661 attcgagttc tgacacacgt tgcgatttcg tttgaccgta ttgtaggact gtgttgaaat
     9721 ttgctttgat cgtgtccaaa tcttgaaacg cgttgcttgc tgcgtagccg ataacgccgc
     9781 tacctagtgc tagtccaatg attactgttg tttttgctag tttgtttttc aatgttgttt
     9841 ctcctttata tgtttatctg ccgatttaat cggttggcgt atagcagttc ctggtacctt
     9901 tttaattcgt gaatcaattg agcgcgttgg tgatcgctca tttggttttt cttgtcgttt
     9961 agttcgtcgt gtagtgatct aatcttttct tttgctaggt cgtggatgga tagttcctgt
    10021 tgtagtgtgt aggtcatagg ctatttaccc agatacactt ctgacatgcc gagtaaatca
    10081 gcaacgttat atagtagttc tcgaaccatt tcttggacat cttctgttgt tgctggctta
    10141 ccatcaactt ttattccctt tgattctgca atttcgatag cagctttcaa agcgtctttt
    10201 acgttttcgt gttccattat ttctcctcct tcatcatctt atcctcaaac cttttgtttg
    10261 gacgagttca gcaccaggaa tatctccgtg cttcagttcc tccttcaatt gctttttatc
    10321 caattttgga ggcacagggg taaagaatcc ttttggaatt aagttctcat tgattacatt
    10381 gaccgatact ggattatttt gaattccaat gttgaataat tcacctttaa tcttcgtttt
    10441 accagtcttt tccatttcct cttgtaaata gtgcttgata ctcttagcat tgttcaaaag
    10501 cgatgtctta cgttcctgta gacgtttaat ttcactatcg attaactcag ctttcccttc
    10561 tacttcttta actatttttg ctaagttttc tgccttatac tcgatcgctt cattaatcga
    10621 atcgagagta tcgcgaagaa tttcttcatc caattgttct gccagttcca ggactttgat
    10681 atatgactcg ctgagttggt aaagagttgc cattcttttg tgcctccttt aataattttg
    10741 caatttgttc aaaagctagg attgcctcat ctaaatccaa ttccacagaa tcatcaattt
    10801 gttcgaaagc aaggtttgtt tcttcaatat cacttgcttg atagatacca atttttccat
    10861 tgtcataaag atcaaatact aaaattcctg acgcatctat attgcgcagt ttgtattcgt
    10921 cttttaaaaa gatgcggtct agtgtatccg ttgcaattag catttacggt tcattccttt
    10981 ctgtggtaaa atataaaaag ataatttatt tcctcgacac gatcatgctt gccggcgttc
    11041 gtgtcttttt ttatttttca aaattactaa ttcttgcatt atttcttctc tcctttttga
    11101 tataatttga gtaaaaaggt ggtgaattac ttgataaaaa ttttgagcga ttaccaagtc
    11161 gctattactt taatcatttc tatttcagga ttttttttat ccctctataa cttatttaaa
    11221 gataaaagaa aaattacgct ctcctatttt ttagttaagc atgatagaag taatcgaatg
    11281 attcttactg gcgtaattgc aaacccatca aaaatgccaa actcaattat cgaatgtacg
    11341 tatctttaca acggtaagaa aattaattgt tcacattatc acgccaatgg ttttgatatg
    11401 ggaagcattc ataaaagtag ccttttatct cctataccgt tggcacaagc aatctctcct
    11461 ggctactcag tcgctttttc agaagtttta gatatgaaag atatattacc tggtgaaaaa
    11521 cttgtaatgg tcataagaac agctaattat caaaaaaaat ttaagttaaa gctaaaagac
    11581 tcattttaat tgaaaaattt ttctttaata atcaaataca aattaattat tattgatcca
    11641 atcccacaaa acataattaa cagaaataca atatcgtcct tactcattag tcagtccctc
    11701 ccgactggct tttttcgctc tgtactcagc ttcatcaagc ccaatgaaaa tccaaaccat
    11761 gtaaacgatc gtgccgatta atgctgggat atttccccag acactcagca gataaataat
    11821 gattggtgca ctgaatacga ttgttgtatt tagtttgtcc ataaatcccc cttctttact
    11881 gtcattattt ccgaacactt acccgatatt ttattgtgct agaacccatg ctctacattt
    11941 ttctttgtcg taaaattttt gatcgcctat acgtccgaat ggaagtcctt tatcttccca
    12001 cttacgaata gttgcagttg atactccgaa gtatttcgct atctctattt gctttaatac
    12061 acgtttatca actgaggcat ctcttcttgc ttttgcaatt tcatcagtta tgatttcatg
    12121 aatgtagcta cgaagtgctg cctcattttc aggcgttaag attacttcca taatcctgaa
    12181 cctcctatcg aattttatga tcgcgaataa cttccagaat aaaggcgtta acagctggac
    12241 ctttatcttt tccacttaaa acacgctgaa tccaagttct cgatctacca tatgcagtgg
    12301 ccaaatcgta ttctgaaata ttatttgctt tcataaactc tttgatggct tcccgcccat
    12361 tatcgatatt actcacttca cacacttcct ttctttttat ttagaaagaa aattggatag
    12421 aaaagtatat ttttagttga ctgcaaacca tactatagtg tagtatatag ccatagttaa
    12481 ataagcatat aacaagcctt tttatcgcac tcggtcgcca aacttaatgc tataaggtgt
    12541 gtttttactt tgcttttttt ctatccaatt aacttacaaa accaatatac actatagtgt
    12601 tgtttaggtc aacacaaaac tacacttttt tattggtttt ttgtaaagaa aaaaggagaa
    12661 tgctggtatg acagtatttg agcggataaa atttcttgca aaaaaacatt caaaaacaat
    12721 gaaacaagta acaattgatt taggatacag tgaaaactat ttctatagtt taaaaagtgg
    12781 aaaacaacca tccgctgaaa agcttactga aattgctaat tatttcaatg tctctgtaga
    12841 ctatctacta ggaagagaga aaaaagagac ccctaaacat gtggatttat cagaagacga
    12901 tactgtattc tcttttgacg gaaaagaaat ctctaaggag acaatgcgta aagcgattgc
    12961 aattgctaaa gctttagaag aaaatgaata gttggagtga tgggttgtat gtatttaaag
    13021 ttgaaagaaa tgctgagtga gtataattta aagttaatct atatggaaat ggaagaacca
    13081 ggtttttatt atccaaaacc aagaatagta tttttgaatg aaaaactaca cgaagacagt
    13141 tctgaagctt ttcatttagc ccacgagctc ggtcatttca ttgcttcaca ttttgaatat
    13201 tcagcattgt acgataactc tacaactttt cattcaaaat tcgaaactga agctgataga
    13261 atcgctatta tgattctact taatatcttt attgagaatg aattaacaga tgaatctcag
    13321 ttcaaattgg aaaattttat ggagttctat tctatcaata ataagttaag aacagaatgt
    13381 tttaatgttt gccagtctta tttcaagaaa aaatactctt atgcacagta aaaaagcccg
    13441 tgctgcaaca cggactcata cctcatttct gagatcacaa atatattata acaagaaatg
    13501 aggaatattt aaatggcaaa aaaagttatg ggtcaagatg ggaaaatgta taaggttaaa
    13561 aaaccgtttt ataaacgggt atggttttgg gtattaatag tacttttgtt ttttggtatt
    13621 ggtggtgctc taagtggcgg tgaaaacaaa gattcagcta ctgcaacatc aacaagtact
    13681 aataaagaaa ctacaaaaga aactacaaca aaagaagaaa agacttataa aatcggtgat
    13741 gacgttgctg tagggaaaat ggaatacaaa gtaaactccg ttgaagtagt aaaacaagtt
    13801 ggcccatctg tacttcctac taacgcaaaa gacactttct tagtagttga cttatctgta
    13861 aaaaatgcag gtgataaggc tgtgactgtt gatagctcat tcttcaaatt aaaagcagat
    13921 ggaaaaactt tcgaagctga ttcggctgca tctatgtcgg caaatcaaga tgaaaatggt
    13981 aatatcacta actctttctt tatggaaagc ttaaatcctg atatgcaaca aactggtaaa
    14041 atagtttttg atatctctga agcccaagca aatgcacaaa ataatgtatt gcaagcacaa
    14101 acaggttact ttggaacaga gacagtttca attgctcttc ataactaatt agtataaaaa
    14161 aaacacaccc accgtccaaa gtaagatcgt ataactaagg aggtgatgcc agctatttta
    14221 gtccgaacac ttacccgagc gaaaggacga aaaaaaatgg caacattcga acaatacaaa
    14281 aagaaaaacg gtgaaaaatt gtggaagttt caaacttatt tgggagtaga tcccttgact
    14341 ggcaaacaag tgagaactac acgaagaggt tttaaaacaa aaaaagaagc tcaattagcg
    14401 ctgaccaaat tacaattgga atacgaaagt aatggtctaa ataagtctaa agagttaact
    14461 tttcaagaag tatacgatct atggattgta aattatgagc agacagtaaa agaaagttct
    14521 ttcgttaaaa caaaagaaca gtttgcgaat catatattac cagcatttgg tgctcttaaa
    14581 atcaacaaaa tatcgattga tatagctcaa aagttcgcta atgaaaaggt aaaaaaattt
    14641 gtgttgtata gagaattcat caataatgct tcgcgtatat gtgattatgc tattaaatta
    14701 ggatatctac aagataatcc ttttaaaaaa atcacagttc caaaaagaaa ggtctctgtt
    14761 catgaagaag atactttaaa cttttttaat aaagaagaac tagaaatctt tttgaaatca
    14821 gtagaaaaga aaaaagatat tcgtatgtat tctttttttc ggacactagc cttcacaggg
    14881 atgcgcgtag gcgagctctt agctctcaca tggaaagaca ttgattttaa cgataattat
    14941 atcaagataa ataaaactct cgccagagga aaaaatagac gcctttatgt agagcaacct
    15001 aaaaccaaaa attctaagcg agatatacca gtcgatgatg aaactatgaa catcttgaag
    15061 aaatggcgat tagaacaaag aaaatggttg ttaacattgg gaattaatac gttaagcaaa
    15121 aatcaactgg tattttctaa ccagaaaaac gaatatctcc aattatctaa gcctcgtaaa
    15181 tggttagaag tgattatcaa acaaaataat cttaaacgta ttactattca tggtcttaga
    15241 catacacatg ctagtttact tttagaagct ggtgcaaata ttaaggacgt acaagaacgt
    15301 ttaggccact cgtctattca aatcactatg gatttatata tccacattac agacaaacga
    15361 aaagaaaaaa cagcagcgca attcgcaaaa tatatcggta tttaagcaga acgtattcaa
    15421 agacgtattc aaacaaaaaa acagaggcga aaacattgca agcaaatgcc tgtatatcaa
    15481 tgttttcgcc tctattatga aatgt
//
LOCUS       DAFEPQ010000060        15347 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.062, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000060
VERSION     DAFEPQ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.062"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(<1..563)
                     /locus_tag="LP543_002054"
     CDS             complement(<1..563)
                     /locus_tag="LP543_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HBL2322213.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="LP543_002055"
     CDS             complement(750..1400)
                     /locus_tag="LP543_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322214.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="LP543_002056"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="LP543_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBL2322215.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="LP543_002057"
     CDS             complement(2912..3910)
                     /locus_tag="LP543_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBL2322216.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="LP543_002058"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="LP543_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBL2322217.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="LP543_002059"
     CDS             complement(4926..5657)
                     /locus_tag="LP543_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBL2322218.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="LP543_002060"
     CDS             complement(5661..6926)
                     /locus_tag="LP543_002060"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBL2322219.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="LP543_002061"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="LP543_002061"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBL2322220.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="LP543_002062"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="LP543_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBL2322221.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="LP543_002063"
     CDS             12268..13770
                     /locus_tag="LP543_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBL2322222.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="LP543_002064"
     CDS             13772..14509
                     /locus_tag="LP543_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL2322223.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14554..14727)
                     /locus_tag="LP543_002065"
     CDS             complement(14554..14727)
                     /locus_tag="LP543_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322224.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14703..>15347
                     /locus_tag="LP543_002066"
     CDS             14703..>15347
                     /locus_tag="LP543_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2322225.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLA"
BASE COUNT         4519 a         3288 c         2590 g         4950 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg aattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc gctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaat aaaaaccaaa
    14521 aatgcacggt cgccatgaag ggaccgtgta tttttatctt cttattttct cacctaatcg
    14581 tatcagccgc atcgcttgtt ttcctgcaca aacaagcaat cgctctacct gttccatcgc
    14641 ataagaaagt accatcggtt cattgatgat atcaagtaca tagtcgatac caccctcact
    14701 taattgagct tgatttttct tctccatatt atgtagttgt gggacaactt tctgacaatc
    14761 agcaattgtg tcaagaacgg atcaatcctt tattggagtt gctcgaaagg gtcttctctt
    14821 attacggaca ggctttgctc actgtacata gacaacaaat cattatccta gtcaatcgga
    14881 tttccagagc ctcattgcta tcattgcttg ataaaatcca gacaaagttt aataaaatgt
    14941 atcagttaca gctaaacttt ggaattgggt cgctttgtta tacagagcaa gagacaccgc
    15001 aatctttcct tcatgccaaa caagtttgcg aatggatagc tttccatcaa tctgtgaacg
    15061 agatccgctt ttttgaagat ttagacctcg ggatcgtttt accagcaatc ccgtcagatc
    15121 aacgaacatt atatgtaaaa cgtattttga aaagcctgac agaagaagaa gtacatttgt
    15181 tcaagaagac tttggcatgc ttcagtaaaa ataacggaag catcaaaaat tgctcagaag
    15241 aattatttat tcataaaaac accttgcaat atcgattgaa caagttccat tctctcactg
    15301 gttatacccc tagaaactat gacgattatc atattctaaa gctggct
//
LOCUS       DAFEPQ010000061        14857 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.063, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000061
VERSION     DAFEPQ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.063"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(42..728)
                     /locus_tag="LP543_002067"
     CDS             complement(42..728)
                     /locus_tag="LP543_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBL2322226.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            808..2346
                     /locus_tag="LP543_002068"
     CDS             808..2346
                     /locus_tag="LP543_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBL2322227.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            2466..2933
                     /locus_tag="LP543_002069"
     CDS             2466..2933
                     /locus_tag="LP543_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBL2322228.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            3452..3895
                     /gene="rplM"
                     /locus_tag="LP543_002070"
     CDS             3452..3895
                     /gene="rplM"
                     /locus_tag="LP543_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBL2322229.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            3911..4303
                     /gene="rpsI"
                     /locus_tag="LP543_002071"
     CDS             3911..4303
                     /gene="rpsI"
                     /locus_tag="LP543_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBL2322230.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(4392..5558)
                     /locus_tag="LP543_002072"
     CDS             complement(4392..5558)
                     /locus_tag="LP543_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL2322231.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(5613..6263)
                     /locus_tag="LP543_002073"
     CDS             complement(5613..6263)
                     /locus_tag="LP543_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2322232.1"
                     /translation="MSIKTLLNLWFLCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            6338..6541
                     /locus_tag="LP543_002074"
     CDS             6338..6541
                     /locus_tag="LP543_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2322233.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKSK"
     gene            6549..6875
                     /locus_tag="LP543_002075"
     CDS             6549..6875
                     /locus_tag="LP543_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322234.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            7030..8073
                     /locus_tag="LP543_002076"
     CDS             7030..8073
                     /locus_tag="LP543_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL2322235.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKNKMQMFPNFYYLLKPVLCKDDVYKINKITHQENSQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLFESLS
                     TKNNKLDEELIETFIEENLLKQMWGESIVNCLKLASNSDYRQFDNWYKKFNYAIKSAE
                     KEQKVQLKIIYEICNNSYFVDHVREQLAMTLRDLIRRAKTDHRIKQKNNYIFTSLKNK
                     ALELIKLQKKEGI"
     gene            8075..8293
                     /locus_tag="LP543_002077"
     CDS             8075..8293
                     /locus_tag="LP543_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322236.1"
                     /translation="MIENHLVDKIHCLDQNADTLELTESICSNTIVLDLKAVNDDYVT
                     HIIIDRKIAQKLSESLRKWAEGGNNENN"
     gene            8280..10925
                     /locus_tag="LP543_002078"
     CDS             8280..10925
                     /locus_tag="LP543_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322237.1"
                     /translation="MKIIKKKIANRKTNWLPVIFLVLIAVILFTSNIAEGSIFNWFDD
                     TESQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAVVDGIQNMIPEVLDLF
                     SFVESTGLNKVYQAVLSTIVVGLMILSLVIIGYKMITGKGTVDLKSVGMNVVMSISLI
                     LLMPTLIHSGIEFSKTFYNDTTTITNSDEGVAWSLIEQGVTDLAYINKTDQYSMIDKT
                     NDRNNLTKENFWKTDLTQVLTDNVLDKMEKENPIADNLRYELVENSDNEFVATKFSDN
                     FLSTFVEGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRVLGVLV
                     FATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFVNYLGTGSGSSVNPFIKAVA
                     YVCAVIVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRSKGGKN
                     SGGNDQAEGEERKPERNFGQSLSQKANKTGKTLGYARERGLSGLASDGATMASERATK
                     PFKSMRDMANDTKNKFKEGLDDGTVYAINKNSKPIVAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNINPASAIQQKVKQDIEERKNAMHGQAKS
                     AEVLINQKRQEAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGL
                     EKSVKENLQNSTSSQDRRSVDVRENLQNTTSSQNGNRTVYVRENVQSSGTSQPRTVDV
                     RENLQNTTSSQNGNRTVDVRENVQSSGTSQPRTVDVRENLQNTTSSQNGNRTVDVREN
                     VQSSASNQPRTVEVRETRAEASQGDKTQKINIIEERKERPNEKVIVEQEVKEKKRPRF
                     TYENNPLFQDISDKPNPLFDSLFKK"
     gene            10939..11127
                     /locus_tag="LP543_002079"
     CDS             10939..11127
                     /locus_tag="LP543_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322238.1"
                     /translation="MQLILQISTQDFLELAELCDSVKKATNEKITVTNYIELMIQEEL
                     MMKNEIESNFKCCSLEKM"
     gene            11139..11750
                     /locus_tag="LP543_002080"
     CDS             11139..11750
                     /locus_tag="LP543_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBL2322239.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVALIKQLNQIGNNLNQAVKALNIIAKYFREQSENKKEK
                     SLHFSFDVSRYNIFKDELANSIAKKMMISYPDDIFLESNRSTSSFLKLSEVEKDYFQY
                     WLEKQTRPDTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            11735..13126
                     /locus_tag="LP543_002081"
     CDS             11735..13126
                     /locus_tag="LP543_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBL2322240.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDNSEE
                     ALQEMNLVRTRMNKSEDIAQAFHVIHSFDKNISKELSIEKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNIDMETCKRLRIDPKDLERMRKINDEVLVENGLEPVDNQY
                     YENLEKRTNLYSNDPSKLKNGKINYQEFVQNAIEATLENCEVNSFVRFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQDVNFYERRDELITLGREKQSRKEKSQYVERTFSARKLGK
                     KYSFESVFERLEKNLEETHMNNFVDLDTEQIIALASGVDYKTGELNVPFVELDSIAQK
                     QFSDHEGAKKIIKYDDFLREVDRHYANTISLEDDQEYTIKLHTDVNPRTLRSTKSKTI
                     LVKKTDQKELFLPAEGFKNCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQRRQRDFEMDF"
     gene            13342..13644
                     /locus_tag="LP543_002082"
     CDS             13342..13644
                     /locus_tag="LP543_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322241.1"
                     /translation="MSDPYIKVYCSKETKTIFDSFYEQNKGIFGTKANMFRVMVSNLP
                     VLANQSNVKLSNPESKGIEQKISEIESIVVGKLDEIDQKLSYYLKNKCKTEEGKDV"
     gene            13637..13984
                     /locus_tag="LP543_002083"
     CDS             13637..13984
                     /locus_tag="LP543_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322242.1"
                     /translation="MFKVYFPFQWLFKGILLLLLGLILFGSYLTIKARYFPEEKPVKI
                     VTKDLKMGETYQADGVEITFKKVELTEKRNEFAQHDRVLQVTYTMKNDTENPIRPQQW
                     SIENKRLFVKSCW"
     gene            complement(14044..>14857)
                     /locus_tag="LP543_002084"
     CDS             complement(14044..>14857)
                     /locus_tag="LP543_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HBL2322243.1"
                     /translation="FLPTVLCMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYK
                     LTQYFLRFPRKARLKVKYLVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQL
                     RVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVD
                     ELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHRYQS
                     GISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         5286 a         2371 c         2922 g         4278 t
ORIGIN      
        1 tatatcagag gttgtgacaa gtcgtttgtc acaacctttt ttcaatacct aagatgaagg
       61 acaatcagca acgcactgct caaaaaagga atgaaaggca gcttttccaa ctttttttta
      121 tgacagataa agtaaacaga aaaaacggat attccaaaca gactagcaag aaacaagatc
      181 tgaagcaact cttctagaga caagccacca ctccaagaaa gcatcaacag agtatcgcca
      241 gctccgagat aggattgacc atagcgaagg cacacaccgc ctacgataaa cgttaacagc
      301 acactttgcc attggaagac gccggcctgc aaccaaaaaa tccataaaat tgcccaagaa
      361 cagaatagcg tgtgagaatg gaccatgaag taaaataaat ccatcaacga aaatagcaaa
      421 gccgaaagca tccatatgaa cgtcgaaaaa tttcttgcgg aagaaaaagt aaagaaccag
      481 gcaaaaagac caccacataa ggcttctgcc aacaaatagt agaagggcaa ccgacacctg
      541 caatagcggc agcgaaatcg ttggacgatg atcgagagaa tcggaatcaa ttcataccaa
      601 gaaagcgaac gacagcattt gacacaatga gatctaggat aaataataga gtgacctaac
      661 ggaatccgct gggcggttaa acataaaaat gatccgatac agcaaccaat gataaaataa
      721 agtaacacgc catctccccc ttttcaaaac caagatacgc aagcttaaac taaatttttg
      781 tacatacata aaatttgagg tgaaaaaatg aacgtttcaa tcggattaga tatcggaacc
      841 acacaaacca aagcagttgc tttcacagat gaagcccaag aagcagcatc cgcttatttt
      901 cgctaccctt tgatccaaga aacacaaggg atggcagaac aagatcagga attgatcttt
      961 caaggtgtct gcaccgttat aaaagaagta attaaacagt tgccttcaga cacaatcatt
     1021 caatttgtct ccttctcctc tgcgatgcat ggcctgatat tgttggatga agagaaaaca
     1081 ccgttatctc gaatgatcac ttgggcagat aaccgagcag cggacgaagc agaaaaactg
     1141 aaatataccg tgcttggcaa agaattttat cggcagactg ggacgccgat tcatccaatg
     1201 acaccattga acaaaatcaa gtggctgcaa aacagccaac ctgaattatt caaaaaaact
     1261 gcttattttg tcggtatcaa agactatatc ttttatagat tgtttggcga actgatcagc
     1321 gactacggaa cagcttcagg aactggatat tttgatatcc atgaattcca atgggcagaa
     1381 gaagtgcttg actacttgaa cattcgaata gagcaattgc cgcaagtctt tccttccacc
     1441 tatcaagtaa caggtctacc atcaaaaaca gcagcagaac taggtttgcc aaatgatata
     1501 tcatttgttt tgggaggggc agacgggccg ctttctaatc tagggttagg tgcttttggt
     1561 gatacgatcg ccgctttgac cgtaggaacg agcggagcac ttcgttttat tactactcag
     1621 cctcagttcc atccagaaat ggagacgttt tgttatgttc tggatcagaa acattgggta
     1681 gtgggaggag cgactagcaa tggtgctggc atctttgatt gggcagggca tacattttta
     1741 caggaagtcg ttcaatctgc taaagaaaaa ggagaaaatc catatgatgc actgctttcg
     1801 gctatcgctt ttgtgccagc gggtgccaat ggtctgattt ttcatcccta tttacttggt
     1861 gagcgagcac cgctttggga cgcagatgcg tttggcagtt ttatcggttt acagcggcaa
     1921 catacagaaa aagaaatgat gaaggcgatt ctagaaggga tctgtctcaa cctttatcgg
     1981 attttaaaag gagtttgcca ggaaaatcag ctcacagaga tccgtgctac aggcgggttt
     2041 gctcaatctc cgatttttcg tcagatgatg gctgatgtac tgggttaccc gcttgttttc
     2101 cctactgtca cagaagcatc cgcattagga gctgtgattt taggctggag aagcatcgga
     2161 aagatccaaa gtatcagtga agcacaggaa ttgatagaat tgaaagagca agttggagta
     2221 caagaaactg atcataaaaa atatcagaaa atctatccat tatttgtttc gacacaaaag
     2281 catttagcac agacttatca gcaatttgct caactgcgag aagaattagc tgattctgaa
     2341 aaatgattgg ctttacggat aaaaaagcga actgaaaatc gttctcaggt aaatgatttt
     2401 gttgtacttt tcattgaaag tctactatta ttttaagtgt aagaaaatgt taggaggcaa
     2461 gaaagatgaa attccaacaa accaaagaag tattaaatca attagttgca gatctaagcc
     2521 aaatgtctgt tgttgttcac caaacacatt ggtacatgag aggaccaggt ttccttacat
     2581 tgcacccaat gatggatgag ttcatggatg atttgaacgc tcagttagat gaagtatccg
     2641 aacgcttgat cacattagac ggttcacctt attctacatt gcgtgaattt gcagataaca
     2701 caaaaatccc tgatgaaatt ggccgctggg atcgtactat tccagaacgt ttggaaacac
     2761 ttgttgcagg ctatcgttat ctagcagatc tttaccaaaa aggaatcgaa gtttctggag
     2821 aagaaggcga cgatccaaca caagatatct ttatcgaatt caaaacagcc acagaaaaac
     2881 gtatctggat gatccaagct catttgggca aagcgccaga gatcgacgcg taaaaactgt
     2941 tgttgaactt gaccgaaaaa gaggttgtga aatcagcgtt ttgacctgag tattagagaa
     3001 acaaacgtca aaatagttcc tcatattttg ccgttttttg agctaatacc gagggtcagc
     3061 tgattgaaca ccgtttacca agaggttgta agcctgccgt tcagctccga gtcacaagaa
     3121 acaattttaa aaaacagctt cttatgtttt atggatattg tgacttgtga ccgaggagtt
     3181 ggcaggagaa caccgtttat tcaaaataag gggttgtgac aagacttttg tcacagcctc
     3241 tttttgtttt tcattttgac aggaaacgac ccttttatat aaaagaaaga aaagaagtaa
     3301 agtttcatat aaatttttca atcaagcaga aaaacgttga cattgactag attagcgggt
     3361 aatatgttaa atggtattgt ttgccccaca aagagccccg gaaactcgaa gtgtatgtca
     3421 aacatcagaa cttaaattgg aggaaaataa cgtgcgtaca acatatatgg ccaaaccagg
     3481 cgaagtagaa cgtaaatggt atgtagtaga cgcaactgat gttccattgg gacgtctttc
     3541 aacagttgtt gcatctgtac tacgtggtaa aaataaacca actttcacac ctcatttgga
     3601 tactggcgat ttcgtcatcg tcatcaatgc agacaaagtg aaattaacag gtaaaaaagc
     3661 gactgacaaa atttattacc gtcacagcaa ctacccagga ggattgaaat caatcactgc
     3721 tggcgaattg cgtgctaaaa attctcgtcg tttgatcgaa acttctgtaa aaggtatgct
     3781 tcctaaaaac actttaggcc gcaaacaatt tacaaaattg aacgtatacg gtggagcaga
     3841 acatccacat gcagcacaac aaccagaagt tttagacatc acaaacttaa tctaatagga
     3901 gggaatttca ttggcacaag ttcaatatat cggcacaggc cgtcgtaaaa acgcagttgc
     3961 tcgcgtacgt ctagtaccag gaactggcaa aattactgta aataaaaaag atgttgaaga
     4021 atacattcca catgctgact tgcgtgaagt aatcaatcaa cctttcgctg ttactgaaac
     4081 aaaaggcgca tatgacgtat ttgttaacgt aaacggcgga ggatacgctg gacaatcagg
     4141 agctatccgt catggtatcg ctcgtgcatt gctagaagtt gatcctgact tccgtttagc
     4201 tcttaaacgt gctggcttac ttactcgtga cgcacgtatg gtcgaacgta aaaaaccagg
     4261 tcttaagaaa gcacgtaaag cttcacagtt ttcaaaacgt taatcgttgt tataccaacg
     4321 atttcagaca ctttctcatt gcgagaaggt gtcttttttt cgttctggta atgagaaagg
     4381 taatgacaaa attataaacc agtgtaagca atgaattttt ctaacgcatt tcgttgagct
     4441 ttttcagtaa catgagaata agtgttaagg gtagtttcag catttttatg acccaatcga
     4501 tcttgtgttt ctttgatact tgctccacct tctagtagaa gagtagcgtg agtgtgccgg
     4561 aatccatgaa tagtaacgtg cttaaattta tatttcttct ctaaacgttt caatatatta
     4621 tttgaatagt ccgcatgcaa aggttgatta atgtcaccgt ctgcatttac ataggtaaag
     4681 ataagttgat tttttgtatt tgtatttatg ccaaactgca aaagctctcg agcttgttct
     4741 tttttccaga tttttaacat ttcaatagtt tgtgcatcaa ttggtaatat tcgttcggaa
     4801 gatatatttt ttgtattttt ttgaatcctg acagtttttc ttcttgcatt cttctcttca
     4861 tttcgaatag ttgcatagct ctttctaaca tacattagct gttcttcaaa ataaatgtca
     4921 gaccaatgaa gagcagctag ctctccttta cgtataccag tagtaatcaa aagctggaag
     4981 attaaaatat gttttaaagg ctcttcttta ttgagaatat cccaaaaagc ttttagttct
     5041 tctaatgaaa agtaattttc attttctttc ttttcccgct ttggtagatt tgtatttgcc
     5101 ataggatttc ggtgtaatag ctctgcaaaa atggcatact caaatatttg agagctgtaa
     5161 gacttaagct gtttgatatt cttgaatttt aaagcccatt cattgactgc attctgacag
     5221 tgaacaggag taatcttaga aatggactta ttcccgaatt ttggtagtat atgcaggcgg
     5281 aaaaggtctt ttgtacgttg atatgtcgtt tcttgtacac gattcctata agaaggcaac
     5341 cattcttcat acactgttat aaacaagtca ttagaaggct tatgatagcc ggtatcttca
     5401 atctcgacct tttttcttgc ttcgtgtaac ttggcttctt tttcagtttt aaaccctttt
     5461 tttacatagt attcttgctt tccattttca tctttacgaa catacaagcg gacactatat
     5521 cgtgcaccgc tttttgttgt gtacttttgt attgacataa ctaatctctc caatctgata
     5581 acttggcgag ttggctgatt gaataattag ttttattgaa gttttgatat taatcgagct
     5641 gttatctctc tttttagatt gttaacctgt acttgggtta agttgccaaa taactcagat
     5701 tctaactctt ttctttcatc gatatttaag tttaaagctt tattaataaa cttcttttgt
     5761 ttggcaaata gaatcgtgta ttcaaaggag tcctctagaa tactttttaa aaaaagaaag
     5821 tcggagtcat cttcttgaag tttgacttca agtaaagaag tcatggaaaa gtatagatta
     5881 gataattcag taaaataatc tgagtcaaga ctggcaatat tcttgactat tttgtccttt
     5941 ttttctaaac gagcagtttt tattctatca agattaggtt gttctatgga atttgtttcg
     6001 tctaatccca tcaaataact tacaggaaca tggaaataat cagcaaggat attccatgtt
     6061 tcagtatttc taggatttcg ctcaccacgg taatagttcg taagagttgt atagttaata
     6121 ccagtttctg tactaatttg tttaatggtc tttttactgg aatcaatata aaattttatt
     6181 ctattcaaag taacaccccc tatagtaaat tccaaactat tatagcataa aaaccacaaa
     6241 ttgagtaatg ttttaattga cattactcgt ttcgtggttt aaaatgtgct tattaagata
     6301 aaaaccacaa agtgaataat ttaaaaggag gcgctgaatg ataactctta atgatcaatt
     6361 cattagaagc ttaagaagac atagagccga tcttatactt actaaaaacg atgcagcaaa
     6421 attgataggt ataaatcgga aaacatatgt caaaatcgaa aatgggagca aagaaagtat
     6481 tagagcatct acttatcaaa agttggtaaa ttggttactt aatgatttga aatctaaata
     6541 aatacaactt ggcgagttgg ctgattgaag aaatagaaaa tgagaggaga aaaataatgg
     6601 atgcaggaat tacagtaatg ttagataaac aacaaacgaa tcaattgaaa aattatgttt
     6661 ttgaaatgac aaaagaggct attgatcaag caagaattga taccgggtta gaaagaccat
     6721 ttctaaaagg aaaagagatg gctaaatatt tgaatgtatc atatacaact tttttgaaat
     6781 ttaaaagaat gggattgcca gttattttat tagaaaaaat ggagttgttt tcaaaagaag
     6841 aatgcaaaaa atggatattg agtcatcaaa tttaaaaaac gaggtgtttt tttgaataat
     6901 gatgaagtag aaaaaattaa aaagagtgtt cccgtcagtt tgcagatcgg gggaacactc
     6961 aataataaag ttctgagaaa actttatttg aatctattat agcaaataaa atcagaagca
     7021 ggtgtgacaa tgaatgaaaa agaagttgaa agcttagttg aaaagcaagg ctattctaat
     7081 tctgatatat atgcagaatt atactatcaa tatcctattt ttttaaacgt aaatgaaaaa
     7141 tataagaaaa tgaaactaag ttctaagtta gtttatatgt tactaaaaga tagaaataga
     7201 ctttcattga aaaataactt gtatgatgag aatcaaaaaa tttttttgat atttgaaatc
     7261 aaaaatctcg tagaaatttc gggaatgtca aaaaatacaa tcttatcagc actagatgaa
     7321 cttcaaaaag ttaaacttct tgtgaaaaga aaaaatggct tcaataaaaa taaaatgcaa
     7381 atgttcccaa atttttatta tttgttaaag cctgtactgt gcaaagatga tgtttataaa
     7441 attaataaga taacccatca agaaaatagt caaaaaaact tgatagttca aaatttgaac
     7501 tatggaacaa atcaatattc tgttgatagt tcaaaatttg aactatgcga cacatcaatg
     7561 aaaacaagag acaaaaacaa ttttgctgat agttcaaaaa atgaactaga attatataag
     7621 tcttataaag atagtaaaga tattaaagaa tcacataatg atgatctttt tgaatcactt
     7681 tcaactaaaa ataataaatt agatgaagaa ttgatcgaaa catttattga agaaaatctt
     7741 cttaaacaaa tgtggggaga aagtattgtg aattgcttaa aactggctag caatagtgat
     7801 tatcgtcaat tcgacaactg gtacaaaaaa ttcaattatg caattaaatc tgctgaaaaa
     7861 gaacaaaaag ttcaactgaa aattatctat gaaatttgta ataatagtta ttttgtagat
     7921 catgttagag aacagctagc aatgacttta cgtgacttga tacgaagagc aaaaacagat
     7981 catcgtatca aacaaaaaaa taattatatc tttacttctc taaaaaacaa agcgttagaa
     8041 ctaatcaagc tacaaaaaaa agagggaatt taaaatgatt gaaaatcatc tagtagataa
     8101 gattcactgt cttgatcaaa acgcagatac tcttgagtta accgagagta tctgctcaaa
     8161 tactattgtt ctcgatttaa aagctgttaa tgatgattac gtaactcata tcataattga
     8221 cagaaaaata gctcagaaac tctctgagag cctaagaaaa tgggctgagg gaggtaacaa
     8281 tgaaaataat taaaaaaaag attgctaata gaaaaacaaa ttggttaccg gttatttttc
     8341 ttgttctaat agctgtgatt ctttttacga gtaacattgc agaaggcagt attttcaatt
     8401 ggtttgatga tactgagagt caaactgagt ttttaactaa tccgctttac cagaattatt
     8461 tgaaaaaata caacggaaat ttttacagcc agttcggtat gtggataggc tggtctgtta
     8521 taaaatcaat gttcgctgtt gtcgatggca ttcaaaatat gattcccgaa gttttagatt
     8581 tgtttagctt tgtagaaagt accggattga acaaagtcta tcaagctgtt ttaagtacca
     8641 ttgttgttgg cttaatgata ctttcactag tgattattgg ttataaaatg attacaggta
     8701 agggaacggt tgatctaaaa tctgttggaa tgaatgttgt aatgtcaatt tcattgattc
     8761 ttttaatgcc aacgttgatt cacagcggaa tagagttttc taaaacattt tataatgata
     8821 cgactacgat tacaaattcg gacgaaggcg tggcgtggtc gctgattgaa caaggggtta
     8881 ctgatttagc ttatatcaat aaaacagatc aatattccat gatcgataaa acgaatgacc
     8941 ggaacaatct aacaaaagaa aatttttgga aaacggatct tacgcaagta ttgacagata
     9001 atgtactcga caaaatggaa aaagagaacc caatagctga taatctgcgt tatgaattag
     9061 ttgaaaattc tgataatgaa tttgttgcta ccaaattttc ggataatttt ctgtcgacct
     9121 ttgttgaggg cattaaatca ggatattatc gctatcaagc aaatctatgg gggatttctt
     9181 ttggattgat tgctttagcg gtagcgtatg tttttagtgc tttcattata attacagcaa
     9241 tactcgagct agcgtttaaa cgcgtacttg gagttttagt atttgctact gacttggaaa
     9301 caggccaacg atcaaagcaa gttttgtcag atattttaca gtgttatttg acagtcggat
     9361 ttcaagggtt cggattgtct atgtttgcaa tgtttgttaa ttatctcgga acaggtagcg
     9421 gttcttctgt aaatcctttt ataaaagcag tagcgtacgt ctgcgctgtt attgtgctga
     9481 taaagggtag tggaacagtc atgcgttact ttggcgtgga tattggttta aaagaaggct
     9541 atggccagct tgcttcagca tttggtatgg gagctatgct gtttcgtaaa ggaacaggtg
     9601 gtatgaaccg atctaaaggt gggaaaaata gtggtggaaa cgatcaagcg gaaggggaag
     9661 aacgaaaacc tgaacgcaac tttggtcaat cactttctca aaaagctaat aaaacgggga
     9721 aaacacttgg gtatgctcgt gaacgaggat tatcaggctt agcttcggat ggagctacaa
     9781 tggccagtga aagagcaaca aaaccattta agagtatgcg tgatatggct aatgatacca
     9841 agaataaatt caaagaaggt ttagatgatg gtaccgtgta cgctattaat aaaaatagca
     9901 aacctatagt agctaaaaac aaagaagatg aaactggaaa atatgctgat tctatgccaa
     9961 cacgtctttc ggatagaaaa gatggcgcta aagttgaaaa cgctgataga attatgtcta
    10021 gttctgaacg tatgcgtgaa gcaatgaaaa ataatgctga atcaaatatt aatcctgcaa
    10081 gtgcaattca gcaaaaagtt aaacaagata ttgaagaacg taaaaatgct atgcacggtc
    10141 aagctaagtc tgctgaagta ttaattaatc agaagcgaca ggaagcaaaa tacacgcctg
    10201 aagcaatgaa tcgtgaggaa atgttaagga aacgtgtaga agggcgtaca ggcgctaata
    10261 tggacgaaaa agaagcgttg actaaagaaa gaatccaaga agctaaaaat tcaaataccg
    10321 gattagaaaa atcagtcaaa gaaaatctac aaaattctac aagtagtcaa gataggcgct
    10381 cagtagatgt tcgagaaaac ttacaaaata ctacaagtag tcaaaatgga aatcgaacag
    10441 tatatgtacg agaaaatgta caaagtagtg ggacaagcca accgagaacg gtagatgtac
    10501 gagaaaactt gcagaatact acaagtagtc aaaatggaaa tcgaacagta gatgtacgag
    10561 aaaatgtaca aagtagtggg acaagtcaac cgagaacggt agatgtacga gaaaacttgc
    10621 agaatactac aagtagtcaa aatggaaatc gaacagtaga tgtacgagaa aatgtacaaa
    10681 gtagtgcgtc aaatcaacca agaacggtag aagtacgtga aacaagagct gaagcaagtc
    10741 agggagataa gacgcaaaaa ataaatatta tcgaggaacg aaaagaaagg cctaacgaaa
    10801 aagtgattgt agaacaagaa gtgaaggaga agaaacgtcc acgatttact tatgaaaata
    10861 atccactgtt tcaggatatt tctgataaac caaatccgct gtttgatagt ctatttaaaa
    10921 aataaagagg tgattgtcat gcaattaatt ctacagatta gtacgcagga tttcttagaa
    10981 cttgccgaat tatgcgactc agtaaagaaa gcgacaaatg aaaaaatcac ggtcacaaat
    11041 tatattgaac tgatgattca agaagaatta atgatgaaaa atgaaataga gagcaatttc
    11101 aaatgttgct ctctagaaaa aatgtgaggg gataatcaat ggatgataaa caaaaaatgt
    11161 acgaaaaaaa tcgcaaacat agtgaggcct taaattttcg tgtcgataaa aagacttatc
    11221 ttgaactgac attgattgca aaacaaagag gaatttctag agcagatgtt tgtcgtgagc
    11281 ggttaaaggg actcaaatat gaaccgccgt tattggctga agatgatagg gtagctctaa
    11341 tcaaacagtt aaatcaaatt ggtaacaatt tgaatcaagc ggtaaaagca ttaaatatca
    11401 ttgctaaata ttttagagaa caatctgaaa ataagaaaga aaagagcctg cattttagtt
    11461 ttgacgttag ccgttacaac atttttaaag atgaacttgc gaatagcata gcaaaaaaaa
    11521 tgatgattag ttatccagat gacatttttt tggagtccaa tcggagtaca tcttcttttc
    11581 tcaaactgtc agaagtagaa aaagattatt ttcaatattg gttagaaaag caaactagac
    11641 ccgatacgaa agaagagtat tcccaaaaag ttgaccggaa tttaattcac tcgattcaaa
    11701 ttattgcaag tttaaaagat gaggtgaaac aactttggga gctactataa catcgaaagg
    11761 acgcaagaaa aatttatcct cgctattaca atatgcagct aatccttcaa aaaaagaaaa
    11821 atgcgttgca gtctcaggaa taaattgtag tgataatagt gaggaagcat tgcaagaaat
    11881 gaatttagta agaaccagga tgaataaatc tgaagatata gcacaagcat ttcatgttat
    11941 ccattcgttt gataagaata tttctaaaga gttaagtatt gagaaaatgc acgaaatagc
    12001 tgtcgaattt gcagaaaaag cttttccaaa ttcgcaaatt attgttgcaa gtcacaatga
    12061 taaagatcat tttcatagtc atcttgttat taataatatt gatatggaga cctgtaagag
    12121 gttgcgtatt gaccctaaag atttagaacg aatgcgaaaa attaacgatg aggtgcttgt
    12181 tgaaaatgga ctcgaacctg tagacaatca gtactatgaa aatttagaaa agagaacgaa
    12241 tctctattca aacgatccaa gtaaactgaa aaatggaaaa attaattatc aagagtttgt
    12301 acaaaatgcg attgaagcta cgttggagaa ttgtgaagta aattcatttg tgcgtttttc
    12361 ggatcattta gcaaaagaat acaacatcga agtttatcaa ttttcaaaaa caaataacaa
    12421 acttggctat gttctttaca aacaagatgt aaatttctat gagcgtagag atgagctaat
    12481 tactttagga agagaaaaac aatctagaaa agaaaaaagt cagtatgtcg aacgtacttt
    12541 tagtgcaaga aagttaggga aaaaatattc atttgaatct gtttttgaac gacttgaaaa
    12601 gaatttagaa gaaacacata tgaataattt tgttgatcta gatacggaac aaattatagc
    12661 gttagctagt ggagttgatt ataaaactgg ggagttaaat gtaccgtttg tggaattaga
    12721 ttccattgca caaaagcagt ttagtgatca tgagggtgct aaaaaaatca ttaaatatga
    12781 tgatttcttg cgagaagtag acaggcatta tgcaaacaca atatctttag aagatgatca
    12841 agaatacacg attaaacttc atactgatgt gaatcctaga actttacgta gtactaaaag
    12901 taaaacaatt ttagttaaaa agacagatca gaaagaatta tttctaccag ctgagggctt
    12961 taaaaattgt tctcatacac cttctgctaa acagattcac tatcaaatca gtcgcataat
    13021 gaatgacgat gtgaaaatgt atcgtgctaa aaaagattac gagttcacgc aacaaatgat
    13081 tgaaaatcag caaaggagac aacgagattt tgagatggat ttctaaagga attagccagt
    13141 agactttcca gtttcccatc gatacggagg gaattggaaa gaggcggttt tgagggcata
    13201 cgccagctac ccatctactt gccagtcaat ccgcccttgc ttacgcaggg ctactttctg
    13261 gcaagttaga tggggaaaac ctctgaggga gcaagctccc tacagactcc ctaaataaaa
    13321 aaagataagg agtaaaaaca catgtctgat ccgtatatta aagtgtattg ttcaaaagaa
    13381 actaaaacaa tttttgacag tttttacgaa cagaacaaag ggattttcgg aacaaaagcc
    13441 aatatgtttc gtgtgatggt atcaaattta ccagtgttag ctaaccaatc aaatgtaaaa
    13501 ttaagtaatc ctgaaagcaa aggaattgaa caaaaaattt ctgaaataga atcaattgtg
    13561 gtgggaaagt tggatgaaat tgatcaaaag ttatcgtatt atctaaaaaa caaatgcaaa
    13621 acggaggagg ggaaagatgt ttaaagtgta ttttccgttt cagtggctct ttaaaggaat
    13681 tcttttattg ctactaggat tgattctttt tggaagctat ctaacaatta aagccagata
    13741 ttttccagaa gaaaaaccag taaaaattgt aactaaagat ttaaaaatgg gtgaaactta
    13801 tcaggcagat ggggtcgaaa taacgtttaa aaaggttgaa ttgaccgaaa aaagaaatga
    13861 atttgcgcaa catgatcgtg tattacaagt gacctataca atgaaaaatg atacagaaaa
    13921 tccgatacgt ccgcaacaat ggtcgattga aaataaaagg ctcttcgtca aatcgtgttg
    13981 gtgaagacaa atttgaataa aacttagtcc gatatgcggc gcgttctgtg cgccgcatat
    14041 tttttagttt tcaaagatca gaccttgttg taaatagatc cgtgcccgaa agttataaaa
    14101 gttgcgataa ccaaacgcaa ttcgtttaat cagcttgatc ttgttattca gcccctcagt
    14161 gacgccgttt gagtgggaca ccttaaaggc atttgaaata cccgattggt agcgatggaa
    14221 gatcgtgaat ttctttttaa acgactgagg taacgtagat gaaattgttt taagggtttc
    14281 ttgaaagcct tccgtacctc gtttaacgaa ataatagcgt aataattgac aaaaatgata
    14341 cgcagttcgc aattcttcgt tgtagcttaa cagctcatca acgatttctg tttgactaat
    14401 tggtcgttta aacagtcgat gataatgccg tttgagtggc tcccatgtcg tggaatcttt
    14461 gagtaagagt ttccaatatc gcttgagccg acggtacttc ttctgatttt cagagtgtga
    14521 ggttcgaaaa cgattcatga tctggacacg taattgattg aagctgcgat tgatgtgttg
    14581 gacaatatga aagcggtcag ttacgatctc cgcatttgga aagactgttt taagcaactg
    14641 acaatagctg gcattcatgt ccatcactag gtacttgacc tttaaacggg cctttctagg
    14701 aaagcgcagg aagtattgag ttagtttgta aagtctgcga tcttcaagga tcgtaaatag
    14761 ctgatgggtt gtcccatcaa cacaaataaa actcatggaa ccagaaacag acttcattga
    14821 tttgaactca tccatacaca acaccgtcgg aaggaag
//
LOCUS       DAFEPQ010000062        14566 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.064, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000062
VERSION     DAFEPQ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.064"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(190..1419)
                     /gene="rpsA"
                     /locus_tag="LP543_002085"
     CDS             complement(190..1419)
                     /gene="rpsA"
                     /locus_tag="LP543_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBL2322244.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(1554..2234)
                     /locus_tag="LP543_002086"
     CDS             complement(1554..2234)
                     /locus_tag="LP543_002086"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBL2322245.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(2283..2918)
                     /locus_tag="LP543_002087"
     CDS             complement(2283..2918)
                     /locus_tag="LP543_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL2322246.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(2984..4387)
                     /locus_tag="LP543_002088"
     CDS             complement(2984..4387)
                     /locus_tag="LP543_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBL2322247.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(4374..5405)
                     /locus_tag="LP543_002089"
     CDS             complement(4374..5405)
                     /locus_tag="LP543_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2322248.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            5436..5657
                     /locus_tag="LP543_002090"
     CDS             5436..5657
                     /locus_tag="LP543_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBL2322249.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(5822..6559)
                     /locus_tag="LP543_002091"
     CDS             complement(5822..6559)
                     /locus_tag="LP543_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBL2322250.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(6712..8052)
                     /locus_tag="LP543_002092"
     CDS             complement(6712..8052)
                     /locus_tag="LP543_002092"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBL2322251.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFLFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(8289..9812)
                     /locus_tag="LP543_002093"
     CDS             complement(8289..9812)
                     /locus_tag="LP543_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBL2322252.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(10190..10801)
                     /locus_tag="LP543_002094"
     CDS             complement(10190..10801)
                     /locus_tag="LP543_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL2322253.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(10924..11049)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(11131..11847)
                     /locus_tag="LP543_002095"
     CDS             complement(11131..11847)
                     /locus_tag="LP543_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBL2322254.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(11853..12422)
                     /gene="scpB"
                     /locus_tag="LP543_002096"
     CDS             complement(11853..12422)
                     /gene="scpB"
                     /locus_tag="LP543_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBL2322255.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(12406..13203)
                     /locus_tag="LP543_002097"
     CDS             complement(12406..13203)
                     /locus_tag="LP543_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBL2322256.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(13173..13544)
                     /locus_tag="LP543_002098"
     CDS             complement(13173..13544)
                     /locus_tag="LP543_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBL2322257.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(13563..14450)
                     /gene="xerD"
                     /locus_tag="LP543_002099"
     CDS             complement(13563..14450)
                     /gene="xerD"
                     /locus_tag="LP543_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBL2322258.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4089 a         3148 c         2465 g         4864 t
ORIGIN      
        1 cattgatttt caactaaaaa agatcaagag gaactgatcc acttgatctc caattattct
       61 aagatttctc tttaccaaca tcatttgaca aagagccttt ttattcctta ttgaaatact
      121 aatcgctttt caaaagaaaa actgagacaa aagcagctgc tttcatctca gtcacagata
      181 caaaatcaat tatttttcag aatcatcggt tgctttttct tctacttctt ctttcaacgc
      241 atcacctaaa atgtctccca tagtaaatcc agtattttct tctggtaatt cataagactc
      301 aacatttttt ggttcttctt ctgaagctgg tttttcttct aaagctttga tactcaatgc
      361 aatacgatgt tcttctggat ggatctctaa tactttcact ttcacttcgt caccttcatg
      421 aagcacttca tgtggtgtcg caatatgttt atgtgaaatt tgagagatat ggactagccc
      481 ttctactcca gggaacactt caacaaaggc accgaagctt gtcaaacgtt taactgttcc
      541 gtctaatact gtgccaactg gagcttttgt atcgatttct gaccaaggtc caggctgtgt
      601 gtctttgatt gacaaggaaa cacgttcttg ttcaggattg atagacaaca ctttgacatt
      661 tacatcatca ttgacagcca atacatcact tggttttgct acatgactat gtgaaatctc
      721 tgagacatgg actagaccgt caattccgcc taggtcgaca aatgcgccaa aatcagtaag
      781 acgtgcaaca cgtccactga gaacatctcc ctcatggata gttgaaagaa tttctttttt
      841 cttttcttct ctttcgtttt ccactaccgc tttatgcgat aagatcaaac gattttctga
      901 tggttcgatt tcgatgattt taaagctcaa tgttttgcct ttatagtctg aaaagtctgc
      961 aacaaaatga tcttccacca ttgatgcagg gacaaaacca cgtacaccga cgtcaacgac
     1021 taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg accttgccct cttggaaatc
     1081 tctttcgata tcttcccaaa cttttttagc atctaaacgg cgttttgaaa gtaggtagct
     1141 gccgttttct ttatctttgc cgatcgtact gataacgact aaatctaata catcaccgac
     1201 ttttactact tcgctgatat cttctaccgg caaagttgat aattcttttg ccggaacgac
     1261 tccttcaact ccagcccctt cgattccgac aatcacttgt ttgtcttcaa tgactaaaac
     1321 ttcgccctta acaacatcgc caacttttac ttcttgtacg cttttcattg cgtcttccat
     1381 tgactggttg ttctcttgat tttgatcaaa ttctgtcata acacttgtcc tcctaaccaa
     1441 cattctgccg taagtacggt actgttttta gtttaattga ttattcaaaa aaaataaagc
     1501 gatttcgttc gttttccttg aaaaaacaat tgtttttcat tttttttctt aatttaaaaa
     1561 agaagataac cttttttcaa aacaacagct tttattgctg caacgacttc tggaatcgat
     1621 ttgccagtcg tatcgatacg aacagcatcc tctgcttgct tcagcggaga gacgtccctt
     1681 gttgaatcat aatggtctct ttcctgtatc tctttcgtca gtgtttcaag atcagtcgga
     1741 attccttttt cttggttctc tttgaaacgt ctttctgcac gctcacttac gcttgctact
     1801 aaaaagattt tcacttctgc attcggtaat acagctgtcc cgatgtctct tccgtccatg
     1861 acaacgccac cgcgttcccc tatttttcgt tggacagcaa ctaattcttc tctgactttt
     1921 ccatgtgcag agacttcaga aacagctttt gtgacatctg gttgacggat ttctaaagtc
     1981 acatcttttg atgctatgaa tacatgctgt ccatcttctt gttgcgaaaa agagatagga
     2041 tactttttga tcagcgaaac tagcccttct tcatctgaaa aagatacatt gttcgtaata
     2101 gctaaataag ttactgcgcg atacatggca ccggtatctg tataaatata attcaattct
     2161 tttgctaata tcttggcaac ggtacttttc ccagatgaag caggcccgtc gattgcaata
     2221 ctgatttgtg tcatgatata actccttttt ctcagaaaaa aagccgcaaa gcgcggctat
     2281 tattatttaa ttcttaattc ttgaccaggg taaagcataa agttgtttgg atctaagcca
     2341 ttcaattcaa acaattgatc agtggtgatc cctgcacgct cagccacttg ttttggacct
     2401 tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg atgaagaagg tgttgtttct
     2461 gctgcattgc tgcttgaatc ccctgcttga taagttgcct cacttgagga agtcggtgtt
     2521 gtctctgctg caatactgct ttctgacgtt acacctgtat cttgagacac agtagattct
     2581 tccgcgattg tactagattc tactgttgaa gtctcagttg actcctcttt tgttgtactt
     2641 gtagaacttt ctactactga tgtcgaagtt tcagaagaag ttgctgctgt tgcattgttg
     2701 cttgatccat ttctgatcca gaaataagca cctgctggaa tagcgataca taagaccagt
     2761 aaaatcaaaa atagcgaaag aaataatgta tttcctttct tttgttgtct ctgagaactt
     2821 cttgatgaaa cttcatcatc tgtatcgtag attggttgtt cccatggttc ttgtgtttcg
     2881 ttattttctg tagattgatg tctatctttt ctgctcactg ttttacctcc taaactctat
     2941 cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa aaatcaatgt tccatgtttt
     3001 ttttaaataa atttagtaaa atcgtttccc aatgaagttg ttcagtctta gaaaaaagct
     3061 tcctctttgg cagaagttct gcttttgctc catcaatatc acaacaattc tgcggctttt
     3121 cggagagttt ttcgccaaaa taagctaaga tgaattttct gcggcagttt tcttcattaa
     3181 tatagcgaag catctgctgc aggcggaatt ctttttcttt ttcctgacgt ttgagccgtt
     3241 ccacccaatt ttctggtttt tcagactgct ggatcagctc catccatttt ttttgcagct
     3301 catcaaaggg agcttgcttt gtggcatatt ccaaataaag ctcaaaactt tgtcgctgtt
     3361 ctctagaaag ttgattaaag aaataatgga tacgttcatc cccactttga taaaaaagaa
     3421 ttgctgcact ttcttcctga tctctgcctg ctcgaccgat ttcttgtaca tagttttcta
     3481 gactgtctgg caaatcataa tggaccacat aacggatatc agatttatcg atccccatac
     3541 caaatgcatt cgtagcaatg agaaactgca gctgattttt tacaaactgc tgttgaagtt
     3601 gtcttctttg agctgcatca agaccaccgt gatagtatcc aactgtaaaa cgttccctga
     3661 ataaatgata caatcgttcg acttcttttt ttgttgcaca ataaatgatt gccgctcctt
     3721 gtgctctttc catgaagtgt tcaagttctt gttctttttg ctgcgttttc cgaacaaaaa
     3781 gcgagatatt ttgccgattg actgattgtc tgatctcttt tggtggtcga tcaaatagta
     3841 catgacgtat atctcttgct actaaatctg ttgctgtcgc cgttagtgcc agtgtgaccg
     3901 ggtttcctaa aagctttcgg atttttccta actgttgata ttctggacgg aaatcaactc
     3961 cccattgaga cacgcaatga gcctcatcta ctacatataa cgcaatattt tgttttttca
     4021 gatgctctaa aagagaagga ttagtcaaca tttcgggact caaaaacaaa aatttatatt
     4081 gcgatagatg acggaggacg tatcttcttt cagattgcga aagtaaacta ttgaaagcag
     4141 ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg atcttccatt agagaaagca
     4201 gcggtgacac gatgatgacc agaccttcca ttaaataccc cgtcaattga tagcaaaggc
     4261 tttttcccgt tcctgtaggc aaaatagcga gagtatcttc tccatcaagc aacgcctgaa
     4321 taatttcttc ttggccggga cgaaatgaat caaatccaaa atattttttt aattcattta
     4381 ttaacattct gtatcgcttc ctttctcaat agatagattt gatacaaacg aaattctcca
     4441 taatctaatt gtccatctac attccattcg ctaaagcgcc actctcgcac ggaatcctta
     4501 tttggttcca aacgttcgat tgtttctgca gagagaatcc actcgtatgg aaaatcatca
     4561 aaaaacaagg cccactcaat aagatgatct gttactgttc ctctttttaa atgacggatt
     4621 gccatgatct ggtcaattgt ctgaccattc ataaacatct gcttggtgat catcatgctt
     4681 tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt gccaaagcga atctggccgt
     4741 ttttcgattt gtgctaacaa aaggtggata catctgcttt gttgcagata aacctctgac
     4801 tcatcattgt tttttgccag ctgataagga agcagccctg ttcgctcatt tccagataat
     4861 tggttagcta aaaaatctgc tgcttcttct ggtatctccg aaaacagctg caccaaactc
     4921 tcatacgcag tatcaatcaa aatttcacgt ggcaattggc tttcactaag ccattttttt
     4981 agttggaaag tataaaaagg acttgtctct gctggaagat agtcttgaat acccgttgcc
     5041 agattagaaa ttacttgaac agcaaattta atgagccgcc aactagtttc ccctgttctg
     5101 ccatagcggt caaaacgcaa accagtatgt tctatttgat gttcggacaa catatcagcc
     5161 cctgcaggcg ttagctttgc ttcattttca tttatttcga tccaaccttg ctgcgcaatt
     5221 ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg gaaaactgtt atggataaac
     5281 aaaagttcgt gggtaaatcc ataaataagt actgaagtcg ttcgttttcc cacaaggagt
     5341 tggtaaagag aactaatccg cagcttgtgt cctgatgaaa atagcgataa aataaaaaca
     5401 atcatattat tctccttaag ttaggatggt tagaaatgtc actactatgt aaacttgtac
     5461 ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc gccagatatt ttcgattatg
     5521 atgaagaagg aatcgtttta tttgcccaag aaccagaggc tcatcaacag ttcgtgatgc
     5581 cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg tccagttcga gcgattttat
     5641 tagaaaataa aatataaata taaacgaaac tttaaaaacg tacactaagt tctctaaact
     5701 tgaaaataag ccgggatctg tttctcttgt tccgtgaaat cccgacttat tttgaaagat
     5761 tcatttgcta tctatagtta attgactagt tttttcacgg accctatagg acccatttat
     5821 attatttcaa ctttttcagc cagttttcca atgtattgac gatcacagtt tgctggtctg
     5881 cgttagaaat ttctgcctct ccatctcctt tttgctcacc atagctgcca aaacctccat
     5941 gatttccgcc attgattgat acatactctg ttctttccgg taaatactgc ttagcttctt
     6001 gataagcttc ctgattcaat actttatctt ttgtcgccgt gatcgataaa acaggaacag
     6061 ctgattctgt caatgttcct tttttgtcgg gataacttgc catgaagaat acccctttta
     6121 aatgtttgtc tgaatgattc ttattgacat aacgacttgc cattacgcca cctaatgaat
     6181 ggccaccgat cacataatcg gaatttggaa aatcttcttt gaccttatcc gctttgtttc
     6241 cgcccatcac tgctagatca agtggcattt tgacaatagc gactgaaaag ccgttttttg
     6301 ctaaatcttg cgcccaaata ctataacttt ctggctctac gagagcacca ggataaaaaa
     6361 taagtaatgg agactttcca tgattctttt tctctccttc aaaaaataag taatcttctt
     6421 tgttttttcc ttcttttacc aacacttgcg cttctgacga tggttcgtac gtattttgct
     6481 gtatatagta ataaccggct ccaccgagta atcctaaaat gatgactaag ccaattagta
     6541 ttcgtctaaa tattttcact ttctcccacc ttttctatct gagtgacccc tttttcttaa
     6601 tcaagtatga ttatacctta aaaaaataag agagaaatgc aaactttcca gcatcaaata
     6661 aaaaaagacg gaatatccat ttgaatatcc gccctttttg tatgtttatt tttatttagc
     6721 acataaacga tacacagctt ctgcataaat ttccattgct ttatacatac tacttaattc
     6781 ccatcgttca ttcgcctggt gcataaagtc aggagtatca gggaacatcg caccgaatgc
     6841 cacacactgg ttcatcgtac gagcaaaagt agcgccgcct gatacaaacg gttcagtcat
     6901 gtctccagta atatcgcgat atgtccccaa tagatttttc actaactcac tgtctagtgg
     6961 cacgtataat gaatcaagga agtcaaattc ttcataagtc aacccatatt tttctgctgt
     7021 tttagttaat ttagctacta aatcatcttt cttgaatgta acagggattc tcatatctac
     7081 accgattttt gtttgttctg gtgtgatttc caaacttgca aagttcatcg ttagctcgcc
     7141 ggattgttca tcttctgttt tgcccacgac agcttcacca gttgcatttt cttgaactaa
     7201 ttttcctaag aaattgattg ggccaaaatc aaatacttct gaaagagcaa ttgccagacg
     7261 agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg atacttttac caagaacgac
     7321 gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt ttcaatgctt cttttacttc
     7381 agaaagcttt tctccagagt aaggagctgc atcaggaaca acattcaacg ctcctccagc
     7441 ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa gcttgcaata agcctttttc
     7501 tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct tgcgtaattc cttcttcttt
     7561 ttcgttatat ttttctaaac aacgccataa attttcttca tctgtaccaa agagaaaacg
     7621 aatacgtgtc ttgaattcca cgccagcatc aatcaaagct ttgacggcat acattgccgc
     7681 aatagaagga cctttgtcat cttgggatcc gcgaccaact aaccagccgt cttttatagt
     7741 tggatcgaat ggttttgttt cccaattatt ttcgtcccct gctggaacaa cgtccatatg
     7801 acacaagata ccgaataatt catctccaga gccaatctct gaataaccgt aatagccttc
     7861 tggatcttta aatgttctaa atcctagctt ttcactgatt tccaatactt tatccaaagc
     7921 ttcaatcact tttttaccga agggatgtcc ttgtcctgaa tctgcttcat ctagtacgga
     7981 aggaattcgg atcaattcgc tcaatgattc taatgcttgt tcatgatgtt gttctgtcac
     8041 aaattttttc atgatattat cctcctaata tgttaaaaag ggtcatgtca aattcctccg
     8101 acacaacctc ttcttctcaa ataatgacgt tttaagcctg ctccatcagc aagcagaatg
     8161 ttcttaacaa atgatgaaag aaaaaacact cactgaatgg agctattgac ttgtccacgt
     8221 gcttatcgat agccgtttat cggtttcttc attcttatga tagaccttgt acctgtattt
     8281 gtctaggttt agaacattgc tgctacacct aggatcacac agatggctac aaataatatc
     8341 gcaattaatt taccggtaaa tttccaccac actgaaacat cgaaacgagc aattgctaca
     8401 gcacccatta cgatagcaga tgttggtgta attaagttta ctaaaccaga tgcagattga
     8461 taagcagtga ttactaaatc cttaccgacg ccggcaaatt cacccatagg tcccatgatt
     8521 cccattgttg cagcagctaa gccggaagtt gaaggaatca agaatgacat agggatatag
     8581 aaaataaatg taataatgat gaatacactt gaagataatg aacttaatcc ttgttcgccc
     8641 caatgtaaga tggtgtctgt gattaatcca ttgttcataa ccacttggat accacgagca
     8701 acagctacta cgattgctac accgatcaaa tcagaagcac cagctaaaaa ttcagagaca
     8761 aattcagact ctttcatgcc ataagtgatt gctactacga tggacattac taagaataac
     8821 atagtaattt ctgggaagta ccagtcacct aatggaagca tgcttttacc tagaaccgta
     8881 cctagtaccg gaaggtttgt taaccagttt gtcaatgatt caaaaatcgt gaaattcgca
     8941 ttcaaggttg tccaaggaat caaactgatg atcatgatac caaaagtaat aaagaacaat
     9001 acattaacat gtttttgttt tttcgtgatt acttcttgac gaccgattgc ttcaatatcg
     9061 aaatgcttca tatcttcttt acgttggttg tatactaatg attttgttgg atctttttcg
     9121 atctttgatg cataacgata cacatataca atagaaatag ctaacaaaat aacgaacatc
     9181 aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa cagcttgtga agctacgcct
     9241 gttgcaaaag gatttaccgt tgaagctaga cagcctactt gagaccctac taatacgatt
     9301 gctacagcta ctactgtatc aaagcctaca gccatcatga ctgggatcag taaagcatag
     9361 aatggaatcg tttcttctgc cattccgtaa gtagagccgc ctaatgcaaa taacagcatc
     9421 aatactggaa taagcatttt ttcttttcct ttgaatttct tcacaactga agcaatcccg
     9481 gcatctagtg cccctgtttt gttgataata cctaaaaatc caccaacaac caaaatgaag
     9541 aaagagattg ggattgcagc aggtgtttct ttagttagct cccccgctgg tacacctagc
     9601 atcccattga ttggcgccat gaaaatatcc cacagccctt gaggattttg ttgtactcgt
     9661 tcatatgatc cagcgacgat gttgcctgca tcgtctacac tatagtgccc agctggtata
     9721 aaccaagtaa gtacagcgat tactgcaata ataataaata agaccgtata cgctgaaggc
     9781 atttgaaatt ttttcttttt tgtttgttcc atcttcttct tcctttcttt tccgttagag
     9841 acaacaattt tggctctaat gttattttat caaaaagagt atcagaacaa aaataataac
     9901 acgcattttg taaaactttt gttcaaaaag ttcgtttttc cctattccct cctctttttt
     9961 caggttttta aagctttctt ctttttcatt gtagcgtttc ctttttttta taaacaaaca
    10021 ttttcgtctt ttaaaaggag aagaatttca catttgcaaa gaattgattg atttttatgc
    10081 ataaatcaaa aaaatactca aaacaaacaa ataaaacgtt tacattattg catgtcttgt
    10141 atttttttcc aaaaaaaaag accaagaaat ttcttgatct ttttgatatt tatttgactg
    10201 ctgtatgacg ttgttcgaac tgatgttgtt ttcgacttaa ccatggaaga agttgcgcat
    10261 gcagcactaa aaagacagca ctcacgatga atcctttgat caagttgaaa ggaacgatcc
    10321 caatcagtac atagtttgct aaagacatgc ccagcatctg tgttgcgttc agccctaaaa
    10381 acattaagta aagaggcgta ataacgaaat aatttgcgat actcataaaa atagttagag
    10441 cgatggttcc tgtaatgaca cctaaaaatt tgtttcttcc tttatgctgt ttctttacac
    10501 taaagaaata ataaatcggg aatgtaaaga tcgttgttga taagaaacta gcagtgtctc
    10561 ctaccaaatt atccggagaa aaacctgtag tcagcagatg taatactgaa cgcaagaacg
    10621 ctgttgcaac tccggccgct ggaccgaata agaacatgct gatcaatacc gagatatcac
    10681 taaaatcgat cttcaaaaaa ctaaaagccg gcatgatcgg aaaagcaaaa tactgtaaaa
    10741 ctaatgcgat cgcagcaaac atcgctactg caaccatttt ctgtacacgg gtgttcttca
    10801 tttgaaatcc tcctgttagg actcctcttc agcgaacttt ttgacgtttc tctccaaatg
    10861 aaaaaactct attttccagg gaaaatagag ttagagttat ttgatcaagc gtcgtcaaat
    10921 aagctctatc ttctttatcc agactatact gtcggtatcg gaatttcacc gattcagcta
    10981 tttttttgca aaataggtcg tggactataa ccaccggtcg ggaatttcac cctgcccctg
    11041 aagacgaacc ttatataatt tttgtttaca tattcattat acatatctat tcaaaaaacg
    11101 caacatctaa cacttataaa aagtaagtgt tcattttgag tggttcagca aactgctgac
    11161 ttcttttttg ttcagttcac ggtaatcccc aggacgtagg ccttgtaaag tcagatctcc
    11221 gtatttttca cgttttagtt tttgcacagg aagaccaact gcttgaagca tatttttcac
    11281 ttgatgatta cgtccttcgt ggatcgtcaa ttccacgata ctgtggtttg tcttaggatc
    11341 cactgataaa atttggaatt tagctggaga tgtttttcgt ccttcgatct ttattccttt
    11401 tgtcaaaggt gcaagcatcg attttgtagc gatccctttt acctttgcaa cgtaaacttt
    11461 atccacttca tgttttggat gtgtcaggcg ctgagcaaaa tctccatcat ttgtcagtag
    11521 caagatcccc gaagtatcgt aatccagtcg acctacagga tagatacgtt cttttaccat
    11581 cggcaaaaga tccgtaacaa cttttcttcc tttgtcatct gcaacagcag aaataactcc
    11641 tttaggtttg tacaataaat agtaaccgta ttcttcttga taaattggga cttcatccac
    11701 ttccacagtg tcatgtttcc caacttttat ccctaattcc cttacaactt gtccgtttac
    11761 ttttacacgt cctgcaagga tgaattcttc tgcttttcga cgagaagtga tgccagcgtg
    11821 ggcaattact ttctgcaatc tctccattac aattattctc ctttattcat tccatcaaag
    11881 aatagatctg gtgacacgtc ttcttcaagt gaaatttcca tttcttgtgt atcaggcagt
    11941 tcttctaatg atttcaaacc aaaataatct aagaagtagc cagttgttcc atataatatc
    12001 ggccgacctg gtccttctac tctacctttt tcttcaatca gtttatgggc agctagacgc
    12061 tgtaccgctc ctgaaacctg aactcctcga atctgctcga tttcagctct tgtgattggc
    12121 tgtttataag cgatgattgc taaggtttcg acggcggctt gagataatgt gtttgatgtc
    12181 ccttgcgcat atcgcttaag taagggagcc agctcttttt tagtcgttaa tacataatgg
    12241 ctttccgttt caaaaatatg caaagctgag tgcgagtttt gagtgtaaga ttcttttaat
    12301 tctttcacta aacttgtgac ttctgcttcg ctgagattta acagtccgct taattcttca
    12361 ttactgatcc cttcctcgcc aacaataaaa agaagggctt caagttcact taatttcgtc
    12421 atgttcggta tctcctactg ctggataaag cataattgat tcatagtttt ctttttgttg
    12481 cgcaatgatc tgcccttttt tcatcaattc tagcaaagcc ataaaagtag tgacaacttc
    12541 acttttagaa ggtgactcaa aaaaagaatc gaaagtacag ccatgcgcat ttctttttat
    12601 acgctcttcg ataaatacca ttcgttcttc gatcgttgtg tcatcgggcg tgatcgtttt
    12661 ttccactttt tgagaaaatt tcttacgttc taagattttc tgcatggcac ggatcaaatc
    12721 gatttttttg acttgtcctc gcttcaacgg cttttcagca tccgctaacc catcgatgtt
    12781 cataggttct tttgtgtaat aaagacttcg ttcctctgct ttttctgtca gtctttcagc
    12841 tgcatattta tatttacggt attctaaaag ctgagtgacc aattcttctc gaggatcttc
    12901 ttcccataat tcttcatcgt ctgtgccgaa ttcttggaca ggaagcaatg tcttgctttt
    12961 gattgccatc aatgtagcag ccatcactaa gtattctcct gcaacagcta gctcgagtgt
    13021 tttcattgta tggatataat tcatatattg ctccgtgatc gtagcgatcg gtatatcata
    13081 tatatcgatt tccatttgct gaatcagatg aagcagcaga tcgagaggac cctcgaatac
    13141 atctaattta atattgatct ccgtcaactc attcactctt tcttcttttt ctttgctagc
    13201 cattttccaa tgtaaggctc tgtgatttca gttgcacgga tggagacatc cgggtacttt
    13261 tcctgtaatt catctaacat ttgaaagccc atattctcac cgcgataaga cggattcaaa
    13321 gaaatgtcat ggatgatcag ctgatcgttc gttgcccagt aaataccaat aatcccttga
    13381 atgttggttg aaccttctgg cgtaaagaaa aaaagttcat aatcgtcttc agattcgtac
    13441 gtatcaatct ccttcaacaa agaagtttta cctgtcagtt tcttatggaa actcaatagt
    13501 cccatagcaa ttttacggtt ttctttccgg taatgtgtta acatcttctc accttcctat
    13561 gactaagccc ttggaaaatg ctgcttataa acatccgtca ttcttttctt cgtgatatgt
    13621 gtataaatct gagtcgtaga gatatccgcg tgacctaaca attcctgtac tgtccgcaaa
    13681 tcagctccat tttctagcag atgtgtggca aagctatgcc gcagcgtatg tggtgttact
    13741 tctttgtaaa tcccagcctc tcgtactaac tgcttcagat ttttccagat tccttgccga
    13801 gaaagacctt tcccatgatt attgacaaat acatgcattt cagatgcgtc ctttacaagt
    13861 aatggacgtg cttcgtctaa gtagcgttcc agccattgga tcgcataatc acctaaagga
    13921 atgatccgct ctttatctcc ttttccaact gtctgtacca gtccaagaga aagatgaaga
    13981 tctcccaatt tcaatccaat caattcactg acgcgcatcc cagtagcata catgacttcc
    14041 aagattgcac gatctcttat ccctaacgtt tttgtcgtat caggtgcttc aatcagcttt
    14101 tctacttctg tcagagtcaa tgtacttggt aatttctgtg ctttcttcgg cgtatcgata
    14161 tgttgcatcg gatcatgatc agtgatacgc tcttgccgta aaaactgatg gaatcttctg
    14221 agacttgaga tcatccgagt aacagttgct gtcgcttttt tttcttcatg caacttctcc
    14281 agaaaacgga tgactaaaaa gcgatcgaca tcctgccatg aagcaatttt ctgctcctgt
    14341 agaaaatgca agtactgttc caaatcacgt tcatagcttt gtcgtgtatt ggcggacaat
    14401 ccccgttcaa tattcaagta gtggagataa tcgttgattt gctctttcat aataatcccc
    14461 ctcatgcctc tctatcatat cagataccgc ttcattctga tagtcttttt ccataatctg
    14521 agcaaagaaa aagccgtcta gacactcaga cggactggtt tctttt
//
LOCUS       DAFEPQ010000063        14449 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.065, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000063
VERSION     DAFEPQ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.065"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            289..2760
                     /locus_tag="LP543_002100"
     CDS             289..2760
                     /locus_tag="LP543_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL2322259.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2863..4599)
                     /locus_tag="LP543_002101"
     CDS             complement(2863..4599)
                     /locus_tag="LP543_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBL2322260.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4596..5300)
                     /locus_tag="LP543_002102"
     CDS             complement(4596..5300)
                     /locus_tag="LP543_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2322261.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5431..5934
                     /locus_tag="LP543_002103"
     CDS             5431..5934
                     /locus_tag="LP543_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBL2322262.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5894..6232
                     /locus_tag="LP543_002104"
     CDS             5894..6232
                     /locus_tag="LP543_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBL2322263.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQILENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6253..7671
                     /gene="ffh"
                     /locus_tag="LP543_002105"
     CDS             6253..7671
                     /gene="ffh"
                     /locus_tag="LP543_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBL2322264.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7783..8361
                     /locus_tag="LP543_002106"
     CDS             7783..8361
                     /locus_tag="LP543_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBL2322265.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8365..8886
                     /locus_tag="LP543_002107"
     CDS             8365..8886
                     /locus_tag="LP543_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL2322266.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8965..9519)
                     /locus_tag="LP543_002108"
     CDS             complement(8965..9519)
                     /locus_tag="LP543_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBL2322267.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9772..10047
                     /gene="rpsP"
                     /locus_tag="LP543_002109"
     CDS             9772..10047
                     /gene="rpsP"
                     /locus_tag="LP543_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBL2322268.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10059..10310
                     /locus_tag="LP543_002110"
     CDS             10059..10310
                     /locus_tag="LP543_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBL2322269.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10435..11226
                     /locus_tag="LP543_002111"
     CDS             10435..11226
                     /locus_tag="LP543_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322270.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11396..11917
                     /gene="rimM"
                     /locus_tag="LP543_002112"
     CDS             11396..11917
                     /gene="rimM"
                     /locus_tag="LP543_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBL2322271.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11907..12668
                     /gene="trmD"
                     /locus_tag="LP543_002113"
     CDS             11907..12668
                     /gene="trmD"
                     /locus_tag="LP543_002113"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBL2322272.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12804..13151
                     /gene="rplS"
                     /locus_tag="LP543_002114"
     CDS             12804..13151
                     /gene="rplS"
                     /locus_tag="LP543_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBL2322273.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13409..13702
                     /locus_tag="LP543_002115"
     CDS             13409..13702
                     /locus_tag="LP543_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBL2322274.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         4847 a         2593 c         2933 g         4076 t
ORIGIN      
        1 tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc gactaaattt
       61 taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta gaaaatcgcc
      121 ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat atgacaggaa
      181 tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct atactggata
      241 ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat gaagaataaa
      301 tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa agtcatccgt
      361 ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg aatagaaaaa
      421 ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat cgtattggaa
      481 aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct ttgtttagta
      541 gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg aatcgccatt
      601 tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa tacagcaaaa
      661 gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct tgtaatagat
      721 gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt cgtggaaggg
      781 aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac caaagaaata
      841 ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc ccgattagac
      901 aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac aatgggagaa
      961 ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat tgggatcatc
     1021 attattccta tcggtatcct gcttttttct caaagttact tttttaatgg aaacacgatc
     1081 aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga aggactgtac
     1141 ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca aagagttctt
     1201 ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg tgtagataaa
     1261 acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc taaaaaacaa
     1321 gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc aaaagcgatg
     1381 gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac cactgatcaa
     1441 tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt agcgttggca
     1501 gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt gccggaatat
     1561 gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt tggtgtgtac
     1621 gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg ctatattctg
     1681 attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa aaaacagcaa
     1741 gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt agctgtccaa
     1801 gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga agaagaatat
     1861 gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca aaagaaaatc
     1921 ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg cgtgaatgat
     1981 attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa tgaagccgcc
     2041 atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc agaagttgtt
     2101 gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt tttagtgaag
     2161 aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac ctatccttta
     2221 gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc tttcttgctt
     2281 gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt gatggaaaaa
     2341 gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat ctgcggggtg
     2401 actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt gattgcagta
     2461 gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag tcagatcatt
     2521 cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct gttttcaatc
     2581 accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc ggcagattcg
     2641 atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa tgaaaaagag
     2701 caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg agaaaattga
     2761 cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt tcgaagcagg
     2821 atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt tcggaatggt
     2881 aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac cattaaggag
     2941 ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat gtcggcttcg
     3001 cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa tgtcgatacc
     3061 tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc ggataataat
     3121 ctcgctgtcg ctttttgaat attggacagc attttcaatc agatttttga taattggata
     3181 aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa tcgtcaaccg
     3241 tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag gttctgtata
     3301 aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga tttcccgaat
     3361 cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag tcagagtttg
     3421 cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg aagtcacagg
     3481 agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt ctaaacgtcg
     3541 cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg aattagtaaa
     3601 gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct cctgatggat
     3661 cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct cttgaatgac
     3721 ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct gcatctttgg
     3781 attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa tcatcagttc
     3841 gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca tcttactcat
     3901 ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg gtgtttgcat
     3961 gattacttcc gcctgattag gattttgcac catttttttt agtacgggca gcacggtttt
     4021 caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa caaggaagaa
     4081 agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta aaaaaacgct
     4141 tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga ctggtaatgc
     4201 gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac ctaggttccc
     4261 accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca atgtttcatt
     4321 gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt cgttcgactg
     4381 atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt cgacatctaa
     4441 ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt ccacgatctg
     4501 ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa ctaaaagaat
     4561 aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc ctgaaactta
     4621 tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg ctcgattttt
     4681 tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa gtcaaaattc
     4741 caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt catgaaataa
     4801 acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac ggtcacttga
     4861 taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc agcttcatct
     4921 tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc taatacttcg
     4981 cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc aatgatccga
     5041 tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt ttcttgacgc
     5101 agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa aatgatgaaa
     5161 tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc actggttacc
     5221 ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg ttcatcatca
     5281 acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg tttatatttt
     5341 agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata ataggaaggc
     5401 actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg tttaggcggt
     5461 ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga actagttgaa
     5521 actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat tcctagagat
     5581 cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc aaaagtatgg
     5641 acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa agcctatcaa
     5701 aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc ttcatgcgga
     5761 acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc cggagtggct
     5821 actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg gctgaaaact
     5881 agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct ttgaatttta
     5941 ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg cagatgattt
     6001 ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat atgacaatat
     6061 caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt tttctaatta
     6121 tattgttcga gaacagatat tggaaaatat gactgcatat attacagata agtacccgga
     6181 agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat aaataaaagg
     6241 aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc gatgggaaaa
     6301 ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg ggaaatccgc
     6361 cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac taagcgcgta
     6421 cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca acaaatcgta
     6481 aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga gttaaataaa
     6541 tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg taaaacgacc
     6601 tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc gctcctaatc
     6661 gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg tcaacagcta
     6721 gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt ccgtcaaggg
     6781 atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc aggacgtctg
     6841 catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac gcaaccaaat
     6901 gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt agctgatagc
     6961 ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg ggatactcga
     7021 ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt catcggttct
     7081 ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag ccgtatcctt
     7141 ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga cgagaaaaaa
     7201 gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga ctttattgaa
     7261 cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat gatcccagga
     7321 atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt tgaacgcaaa
     7381 aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct gttgaaccca
     7441 agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt gaatcgaatg
     7501 atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg ggatatgaac
     7561 atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg caaaatggcg
     7621 atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata agatgaaaaa
     7681 aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca gcaattttta
     7741 ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg tccgtggagt
     7801 gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc tgaatatgat
     7861 gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt gagctggcag
     7921 acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga tatcaaaaaa
     7981 gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg aattattcgt
     8041 aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa aattcaagaa
     8101 gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat cgttgattct
     8161 tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga attagcaaaa
     8221 gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc ttttttagaa
     8281 gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga ggtcgtacgt
     8341 atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc tataagttta
     8401 gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac tccaacaggt
     8461 aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat gggcagaaaa
     8521 acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga gaaaaagaat
     8581 ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt aaacgaagat
     8641 cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg ggttgtgggt
     8701 ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg gtatatccag
     8761 attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac atatgaagcg
     8821 cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca ttatgtacga
     8881 aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg tacatagttg
     8941 ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat ctagatacgc
     9001 atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat tttcatcaat
     9061 tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca cattcaaaaa
     9121 gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac ctggtgatgc
     9181 agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc ttgagcctac
     9241 atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag taacgaaaac
     9301 gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag gagcctgttc
     9361 tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa atacactttc
     9421 gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt aagaatcttt
     9481 tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc tccttcaaac
     9541 tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac ttactgacaa
     9601 aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa atcttctatt
     9661 ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca gggtaaaaaa
     9721 aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat aatggcagtt
     9781 aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat cgtcgtagct
     9841 gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta caatcctttg
     9901 aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt atcaaaaggt
     9961 gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat gcaaaaacac
    10021 cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta agcgacttag
    10081 tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta gacgtcgtag
    10141 agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt ggccgtatca
    10201 ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt gttcggatca
    10261 atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa ataaacgtgc
    10321 tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag aaatgattat
    10381 ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca gccaatgaga
    10441 aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc ggttactcaa
    10501 gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag tccagaaaaa
    10561 agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa taatgagaaa
    10621 aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc tctagatgta
    10681 ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg ttattctatt
    10741 ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa tcaagaaacg
    10801 cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac aatccaagtt
    10861 ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc tcgaatcaca
    10921 gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg aaactggcgt
    10981 attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc taaagaaatc
    11041 gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa aattactgtt
    11101 gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc gaaaaatgcc
    11161 tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc tatgaagagg
    11221 ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca ggaagtaatc
    11281 aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag aaaactagaa
    11341 ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat ataagttgac
    11401 tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg aagtccgtgt
    11461 tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc taacactatt
    11521 ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac ataaaaattt
    11581 tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat atcgggatgg
    11641 catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat tctattacca
    11701 tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag taaaagaaat
    11761 cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag ctgatatatt
    11821 gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag tggtccgtgt
    11881 agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta tttccaagaa
    11941 tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa ggattattag
    12001 atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca gtagatgatt
    12061 atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat gataacatcc
    12121 aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt gacccagctg
    12181 gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac ttagtattca
    12241 tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca gatgaagttt
    12301 ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg atcgatgcaa
    12361 cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg gattcttatt
    12421 caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa gggatgaaag
    12481 taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa ttaaaagaat
    12541 cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa tggacacctc
    12601 aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca gcagatgaag
    12661 ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta gtattttatg
    12721 gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct cataagaata
    12781 tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca aaagaacaat
    12841 tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac gcaaaagttg
    12901 ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa cgccgcggtg
    12961 ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc gttgaacgta
    13021 cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac ggtaaagtac
    13081 gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt atcaaagaaa
    13141 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
    13201 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
    13261 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
    13321 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
    13381 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
    13441 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
    13501 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
    13561 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
    13621 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
    13681 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
    13741 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
    13801 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
    13861 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
    13921 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
    13981 cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa
    14041 caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag
    14101 aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc
    14161 agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg acacatttct
    14221 cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat aatcccgtcc
    14281 taactctttt attcttccct catagcttcc aaatttaagc aatacttcaa ttaatcaagt
    14341 tttatctttt tttttcaata atcgtaaaaa aagagtattt tttatttaaa aataaaatct
    14401 ttgtattaaa ggacaagact aaaataacct tagaataaaa agagaaaaa
//
LOCUS       DAFEPQ010000064        14381 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.066, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000064
VERSION     DAFEPQ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.066"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            142..523
                     /locus_tag="LP543_002116"
                     /pseudo
     CDS             142..523
                     /locus_tag="LP543_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            654..1604
                     /locus_tag="LP543_002117"
     CDS             654..1604
                     /locus_tag="LP543_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL2322275.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2122..2577
                     /locus_tag="LP543_002118"
     CDS             2122..2577
                     /locus_tag="LP543_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL2322276.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2918..3520
                     /locus_tag="LP543_002119"
     CDS             2918..3520
                     /locus_tag="LP543_002119"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322277.1"
                     /translation="MLDPLFTDETFITFGFGHLVELAEPGHYDEKWQNWKLESLPIFP
                     DRYDFEVAKDKGKQFKIVAELLKKANTIIVATDSDREGENIAWSIIHNANAFSKDKTF
                     KRLWINSLEKDVIRSGFQNLQPGMNYYPFYQEAQTRQIADWLIGMNASPLYTLNLQQK
                     GVQGTFSLGRVQTPTLYLIFQRQEAIENFKKEPFFEVEAK"
     gene            3614..3946
                     /locus_tag="LP543_002120"
     CDS             3614..3946
                     /locus_tag="LP543_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HBL2322278.1"
                     /translation="MGNQEGIIADVQTKEKKTNSPSSFSLSSLQSKVNQLYKATASQT
                     LKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIRSN
                     NSTQILTN"
     gene            complement(4097..5076)
                     /locus_tag="LP543_002121"
                     /pseudo
     CDS             complement(4097..5076)
                     /locus_tag="LP543_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5081..5848)
                     /locus_tag="LP543_002122"
     CDS             complement(5081..5848)
                     /locus_tag="LP543_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL2322279.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6338..6763
                     /gene="lacA"
                     /locus_tag="LP543_002123"
     CDS             6338..6763
                     /gene="lacA"
                     /locus_tag="LP543_002123"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HBL2322280.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6780..7295
                     /gene="lacB"
                     /locus_tag="LP543_002124"
     CDS             6780..7295
                     /gene="lacB"
                     /locus_tag="LP543_002124"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HBL2322281.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7306..8238
                     /gene="lacC"
                     /locus_tag="LP543_002125"
     CDS             7306..8238
                     /gene="lacC"
                     /locus_tag="LP543_002125"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HBL2322282.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8241..9221
                     /gene="lacD"
                     /locus_tag="LP543_002126"
     CDS             8241..9221
                     /gene="lacD"
                     /locus_tag="LP543_002126"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBL2322283.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9249..9566
                     /locus_tag="LP543_002127"
     CDS             9249..9566
                     /locus_tag="LP543_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL2322284.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9566..11272
                     /locus_tag="LP543_002128"
     CDS             9566..11272
                     /locus_tag="LP543_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2322285.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11414..12820
                     /gene="lacG"
                     /locus_tag="LP543_002129"
     CDS             11414..12820
                     /gene="lacG"
                     /locus_tag="LP543_002129"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBL2322286.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12843..13115
                     /locus_tag="LP543_002130"
     CDS             12843..13115
                     /locus_tag="LP543_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HBL2322287.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13136..14035
                     /locus_tag="LP543_002131"
     CDS             13136..14035
                     /locus_tag="LP543_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBL2322288.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4695 a         2371 c         2773 g         4542 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgatgacact atcgttcttc atcaacgtaa
       61 gttagatttg ttgaaagagc agaaaaaagg atttctacaa aaaatgtttg tttgatattt
      121 taaatagaaa agagagataa agtgaagttt ttaaattttt tgaactataa atatttaaac
      181 tgggtattat atggtattac attatttttt attatttatt acttttttac cgatgatgat
      241 atttttttaa ttttagcaat actttttctt gtttcatcta attttagtga taacttaatt
      301 gaagttctta cagaaccaga aattcggtct atgacggatg aagaacaaca gattattaaa
      361 gctatgattt cggaaaataa gaaagacgta gtagttatca aaaagattag agagtataca
      421 aatttaggtg ttttacaagc caaaaaagta tatgaaaaaa aaaaaaaggg tctataatta
      481 caaattagat aatacattag aagctggaga caagattgtc tagataatgt gtgagataaa
      541 atttttggca ggttatcata cataagacaa aatgttttta aaactgataa cgtatttatt
      601 ccacaacaat tacgcattta tttctgagaa aaatataaag caggaggatt acaatgctga
      661 aaacttattc cttaacaaaa gatacattat tagtttcgtc agaatcaaat atcaaaaatt
      721 cttctgttgt ttatgtttat ggtgacgaac gagaatatat cgaatcttct gaacataaat
      781 atgactttaa aataggcaat atactaacgt ttgatgatcg agttgcatat gacacgatga
      841 tagataagaa cgatgaaaaa agtttgctcg taagtttttt gtttcccgag gtacatgaag
      901 gtgggcatct agataccctg acgacatctg ttgttttctt ggtatttaaa aataagttga
      961 taatttttac aaaacaaaaa ttaaaattca ttcccgaact cttatccaat atggttttac
     1021 gtgatgtcaa tcatttcgtg gaggaagtac ttttaaagat catgcaaaaa gatttctata
     1081 tgatgcttaa cgaccttcgt ggagtcaagg aaaaaattga tgatttagac tcagaaatat
     1141 caacgtccgg ttcactcaaa ccaacgtttg gtgatttatt gactcttcaa aaatacatga
     1201 tagctttgtc aacaacttat aaagctaacc ataaagcgct tgatttcatt aaaaaaaatt
     1261 tcacaactat tgacgatatt gattttagta ctaaaaaaat aattgatgat ttatacgata
     1321 caagtgatac aatggataga atcattttaa gttacagcca atatttggac aaattggaaa
     1381 atatgataaa caatatgatt tcgtatcagc taaacatgat tatgaaaacc ctaaccgaga
     1441 tatcaattgt tttgactatt ccagcaatta tttttggctt ttggggaatc aatgtccatg
     1501 tcccgtttga aaagtcttct tatggtgttt tagccgtatt gattatctct gctatactaa
     1561 gtctaatttg ctggcttttg atgcgacgca aaacatattt gtaaagtatt tgtgataccc
     1621 taatatgatt taaagcagaa tttctttgtt gactttttaa aaggctgatt cctttcaata
     1681 atttaatagc ttcagcggct gctcagaagt caataaattg gactattttt attcagttac
     1741 ttaggcaaca tttgagatac gattgcgatt ataaaaattg atataccaac caatggctgc
     1801 ttggaagact gacatattag cctcgatatc atcgaataac taaatacaga aaaatacaat
     1861 aaatattatc aaactaggga ctatactaga aaacatcagt acattcctta actcagggtc
     1921 tataattaga aaatcgcccc tttgaaaact gattaaaaca gcccccatta tctagtgact
     1981 agataatggg ggctgttttt ttgttaagtg atataataaa ccataaaaga agtttagaaa
     2041 tacggtcctg gtcatcaata aataaacaat ttaattaatt atttatttaa ttatttaatt
     2101 tcagaaaggt atacatgaat catggtgcaa caaattgtcc tacccatcaa agactccagc
     2161 gtcttaaaga tggtacaaga tacacttctc gatagtttcc gtgctggtcg ccgaaattac
     2221 accgtttttc aagttggtaa ggccacgcta ctacgtgtga gtgatgtcat gacattaaaa
     2281 aaatcagatg tctataatcc agacggctct gtcaaaaatg caacctttat tcatgataaa
     2341 aagaccggta aagcaaacac gttatattta aagcctgttc agcaagactt gttgcaatat
     2401 catgattggc tggcccaaga aaatatcaat tctgagtggt tatttccctc gacagcccat
     2461 tatgatcttc atatcactga gaagcagttt cataaggtga tggcgcatgt tggtgatctc
     2521 ttaggaatca acttggaaga caaagagaaa aaattgcgag tcagaaaacc gttttgagat
     2581 atgaacagca tttgccttca cgccttagcg attttaaata atagccttga aagtgattat
     2641 ggaaaagccc tctacgctca atgcaaaatt gacgtagaaa ctatttttga tcgattgaaa
     2701 ggtgtatttg gtatgtgaag aacccatgtc agaggcaaga acgttgcacc tactgacatt
     2761 ggaatgatgc ttatgagcat gaatctaacc aaattatccc ttgaggctag aagaaaagcg
     2821 gaagcttttc aacacaaagc agccaaaaat aaaaaacgcg acgaagcaat cacatttatg
     2881 attattcatc gcgtttttta tatttagagc tagttatttg ctagacccac tatttacaga
     2941 tgaaacgttt atcacctttg gttttgggca tttagtggaa ttagcagaac caggtcatta
     3001 tgacgaaaag tggcaaaatt ggaaacttga atctttgccg atttttcctg atcgatacga
     3061 ttttgaagtt gcaaaagata agggaaagca gtttaaaatt gttgcagaac ttctcaaaaa
     3121 ggcaaataca attattgttg caacagatag cgacagagaa ggtgaaaata tcgcctggtc
     3181 gattatccat aatgcaaatg ccttttcaaa agataaaaca tttaaaagac tatggatcaa
     3241 tagcttagaa aaagatgtga tccgaagcgg ttttcaaaat ttgcaaccag gaatgaatta
     3301 ctatcccttt tatcaagaag cgcaaacacg ccaaattgcc gattggttga tcggcatgaa
     3361 cgcaagccct ttgtatacgt taaatttaca acagaagggc gtacaaggta cattttcact
     3421 aggacgtgtt caaacgccca ccttatacct tatttttcag cgccaggaag ccatagagaa
     3481 ttttaaaaaa gaaccttttt tcgaggtgga agctaagtaa aacaagggtc atttaagggc
     3541 gttctaagcc ccacacagcg ttttaaaacc caagaggagc tttttagctt ttgtttcttc
     3601 taaacaagct aaaataggca atcaagaggg gataattgct gatgttcaaa ccaaagagaa
     3661 gaaaacgaat agtccgagtt cgttttcttt aagtagtttg caatcaaaag tcaatcagct
     3721 ttataaagcg acagcgagcc aaactttaaa agctatgcaa ggactgtatg aagcaaaatt
     3781 attgagttat ccaagaacag atacaccatt tattacagag aacgaatttg cttatttaaa
     3841 agcgaatttt ggcaaatata gcggtttttt aggacttgat cttgaaatgt tgttatcatt
     3901 gataatgata cgttcaaaca acagcaccca aattttgacg aattagtgaa actctatgaa
     3961 aaagacgtag taaaacatgc aacatcctat tctaatcagc tggtaaagct taattaggtt
     4021 atataagctt gaaaataaag ggtttgtcca atatggacaa gcctttttct tatgttttgc
     4081 cacagatttg ccacacttat tgtgtaagag tatcaaggat aggtataatt tgatactttg
     4141 tttttttagt gatgtgcaaa taaatttctg ttacacgact acctctagaa tgccctacac
     4201 ggtcttgaat tgcttctagg ggtattcctt actctgctaa gaaactaata tgcgtatgac
     4261 ggaatatatg ggtcgtaata tgtttattaa aactaagttt attatcaata tcaattcctg
     4321 ctcgttttgg attacctttt cgatttaatt ttccattttc aggtgagttg aggaaattgt
     4381 taaggacact ataatgaaga ggtgttccat tatagtcggg tttatgacga cttttggaag
     4441 taaaaatcca tccattatct ttataatcag gattgaatct catatgttgg tgatttaact
     4501 caatttcctc tttaagaatt tctatagccc ttttagataa ttggacattt cggtaacttt
     4561 gtaagttttt tacattacct tcatagctat tttctgcagc tcctccgtga gtggactgac
     4621 taatgatgat attttgcttt tcaaaatatt gataggcacc aatattttgg agtttcgact
     4681 ctgtaagtca acagggccat tagttagaat acctattggg atgcggtttt tatgtagagc
     4741 cagcagtagt tgatgtatct caggaatcat cgtaatctga tcgagttcat attcatagtc
     4801 agcttggaag tctagagctt ctgtcctaga aatactatcg atgccaaatt cttccatagt
     4861 cttctgacag cgaaaaattc taagctgctc aatactgatg agttttttcg tgtatttagg
     4921 aaaagcaaca tcagaccaat atctgaaacg tttgtaaatg ttatctattt gttggatatc
     4981 aatcgttgga aaacatttct caacgctacg tttaaagggg agttgatggt cataaagagt
     5041 gtcatcaagg tcaaaaacaa tatttttata agtcataatt ctagtccgct tttaaaatag
     5101 tagtgtactg gctaaattcc tctaagtgtt gagggctaat ctggttatct gtaatgatgg
     5161 tatcgagttg atctagatta tagaagttaa agaaatcgta tcgatcgaat ttagtactgt
     5221 ctactaataa gaatttttct acagcattgt ttagggcaag ttgttgaatc acaccttcct
     5281 tgtcactata tgtagcaata gaattatggg taacagcatt tgcactaaca aaagctttgg
     5341 caaatctaat tgcttttaaa tttgtcgaag ccattgaacc tacaaaagct ccagttattt
     5401 ctctatattc accgccaaga agcaataaat caatggtttc gctatcattt agaatcaaga
     5461 acacagggag actatttgta atgacacgaa ttttataacc tttacgacct ttcaattcta
     5521 atgccagttg ttcaagtgta gttccaggtc caataaaaat agtatcgcca tctttgatta
     5581 aagagcaggc ttttttagca atgtcaattt tttcttttgt atttagactt ttcttctcga
     5641 tatgtgtctt ttcaagtggc tttttagagg aaagcaattg tgcaccacca tgagtacgtg
     5701 ttaaaagtcc atcagcttct agagcatcaa ggtctctacg ggctgtcata tcggaaatat
     5761 ctagttcatc tattatttct tttatggtta tggtcccatc aatctttaac atatctaaaa
     5821 ttttttctaa tcgtcgtttt ttgttcatat gaagaccctt ctttcataaa gtaatttttt
     5881 tccaaagata attctctttt aattgtatca taaaagacaa tattttcaag gtaaaacaaa
     5941 caatttcaaa caaaaacaaa tgttagatga tgaaataaga acagaggatt gacgtatatt
     6001 agcttaggtc agattttgta taagacgaaa ataaagtagg acctcttaat cagtaagtta
     6061 tagaaagtaa aagacttttg taatacttga atagatattt cacgtccatt ttgtgatgga
     6121 ttaaatgaac aaaaatgaac aataatttaa cggtgttatc tattttttaa aaaaacaaat
     6181 aaaaaaaaca aaaaataaac aaaaaaagtg ttgctttttg tttgaatgct tgatattata
     6241 taaacaaaga aatgatgaaa acgttttttc gaacattttg caaaatgttt tctacttcta
     6301 cgtagcattt ctttctaaaa tttaggaggt ggtcaaaatg gctattgttg ttggtgcaga
     6361 tctcaaaggt acgagattaa aagatgttgt gaaaaatttt cttgtagaag aaggttttga
     6421 ggtaattgat gtgactaagg acggacaaga ttttgttgat gttaccttgg cagttgcctc
     6481 tgaagtaaat aaagatgagc aaaacttagg tattgtaatt gatgcttatg gagctggtcc
     6541 attcatggta gcaactaaaa ttaaaggtat ggttgcagca gaagtttcag atgaacgctc
     6601 agcttatatg acacgtggac ataacaattc acgaatgatt acggttggcg ctgaaatcgt
     6661 cggtgatgag cttgctaaaa acatcgctaa aggtttcgtc aatggtaaat atgatggcgg
     6721 acgtcatcaa atccgagtag atatgcttaa caaaatgtgt taagaaaagg agttaaggaa
     6781 tgagaatcgc aattggatgt gaccacattg tcacagatga aaaaatggca gtatcagaat
     6841 tcttgaaatc aaaaggacat gaggtccttg atttcgggac atatgaccac gcacgtactc
     6901 actaccctat ctatggtaaa aaagtcggtg aagcagtagt aagtggtcaa gcagacttgg
     6961 gagtatgtat ctgtggtaca ggtgtcggca ttaacaatgc agtcaataaa gttccaggtg
     7021 ttcgctcagc gttggttcgt gatatgacat cagctatata tgcaaaagaa gagttaaatg
     7081 cgaatgttat cggtttcggt ggaaagatta ctggtggtct tcttatgaat gacatcattg
     7141 aagctttcat tgaagctgag tacaaaccaa cagaagaaaa taaaaaattg attgcaaaaa
     7201 tcgaacacgt tgaaacacat aatgcacatc aagcagatga agatttcttt acagaattcc
     7261 ttgaaagatg ggaccgtggt gactaccacg attaaggtat aacaaatgat tctgacagtc
     7321 acactaaatc cttcagtaga catctcttat cctctagaaa cattgaaaat tgatacagtg
     7381 aatagggtga aagatgttag taagacagct ggtggaaaag gcttaaatgt tactagagtc
     7441 ttgtatgaat ctggtgataa agttactgct acaggtttct taggtggtaa aattggtgaa
     7501 tttattgaaa gcgaattaga acaatctcct gttagtccag ctttttataa aatctcagga
     7561 aacactagaa actgtattgc tatcttacac gagggtaacc aaacagaaat tttggaacaa
     7621 ggaccaacaa tttctcatga agaagcagaa ggctttcttg accactatag caatcttatt
     7681 aaacaatcag aagttgtgac aatttcagga agtttacctt caggacttcc caacgattat
     7741 tatgaaaaac ttatccaact tgcttcggat gaaggagtag cagtcgtttt ggattgttca
     7801 ggtgcacctc ttgaaacagt tctaaaatcg tcggctaaac ctactgccat caaacctaat
     7861 aatgaggaac tttcacagtt gcttggaaaa gaagtaacaa aagatattga agaactcaaa
     7921 gacgttctta aagagtcact ttttagtggt atcgaatgga tagtggtctc attaggtcga
     7981 aacggtgcct ttgcaaaaca tggtgatgtt ttctataagg tagatattcc tgatatccca
     8041 gttgttaatc cagtcggatc aggggactca acggtagctg gtattgcatc agctttaaat
     8101 agtaaaaaaa gtgacgctga cttattaaaa catgcgatga cattgggtat gttaaatgct
     8161 caagaaacaa tgacagggca tgtgaatatg actaattacg aaacactaaa ctcacaaatt
     8221 ggagtaaaag aggtataaaa atggtactta cagaacagaa acgcaaatct ttggaaaaaa
     8281 tttcagataa aaacggtgtt atctcagctt tggcatttga ccaacgtggt gctttgaaac
     8341 gtttgatggc acagtatcaa gatacagaac caactgtagc tcaaatggaa gaactaaagg
     8401 ttttggttgc tgatgaactt acaaaatacg cttcttcaat gttgcttgac cctgaatatg
     8461 gacttccagc aacaaaagca ttggataaag aagcaggtct tcttcttgcc tatgaaaaaa
     8521 caggttatga tacatcaagt acaaaacgtt tgcctgactg cttggatgtt tggtctgcaa
     8581 aacgtatcaa agagcaaggc gctgatgccg ttaagttctt gctttactat gatgtagaca
     8641 gctcagatga acttaaccaa caaaaacaag cttatattga acgtgttggt tctgaatgtg
     8701 tcgctgaaga tattcctttc ttcttggaaa tccttgctta tgatgaagaa atctcagatg
     8761 caggttctgt tgaatatgca aaagtgaaac ctcgcaaagt cattgaagcg atgaaagttt
     8821 tctctgatcc acgctttaac attgatgttc ttaaagtaga agtaccagtt aacgttaaat
     8881 atgtcgaagg ttttgctgat ggtgaagtag tttacagtaa agctgaagct gctgacttct
     8941 ttaaagcaca agaagaagcg actaaccttc catacatcta cctaagtgca ggtgtatctg
     9001 ctaaattgtt ccaagaaaca ttgcaatttg ctcacgactc aggtgccaag tttaatggtg
     9061 tgctttgtgg acgtgctact tgggcaggat ctgtagaacc ttacatcaaa gaaggtgaaa
     9121 aagcagcacg tgaatggttg cgtactactg gattcgaaaa tattgatgaa cttaacaaag
     9181 ttcttgttaa aacagctagt ccatggactg ataaagtata gtaaaaacat aaaacggagg
     9241 atattgttat gaacagagaa gagatgactc tcttagggtt tgaaattgtt gcttatgctg
     9301 gagatgctcg ctctaaactt ttagaagcgc ttaaagcggc tgaaaatggt gatttcgcta
     9361 aggcagatag tcttgtagta gaagcaggaa gctgtattgc agaggctcac aattctcaga
     9421 cagctatgtt ggctcgagaa gcttctgggg aggaacttcc atacagcgtt accatgatgc
     9481 atggtcagga ccacttaatg actactgtct tattaaaaga tgtgattcat cacctcatcg
     9541 aactttataa aagaggagca aaataatgca taaactcatt gaacttattg agaaagggaa
     9601 accgttcttt gagaaaatct ctcgaaacat ctatcttcgt gctattcgtg atggatttat
     9661 cgctggtatg ccagtcatcc ttttctcatc tatctttatc cttattgcct atgtcccaaa
     9721 tgcttgggga ttccactggt caaaagacat tgagaccttc ttgatgactc cttatagcta
     9781 ttcgatgggt atcctagcat tctttgttgg tggtactacc gctaaagctt tgacggactc
     9841 aatgaaccgt gacctacctg cgaccaatca gattaacttc ttatctacga tgctagcgtc
     9901 tatggtaggg ttccttttga tggcagctga gcctgcaaaa gaaggaggct tcttgacagc
     9961 cttcacggga acaaaaggtc ttttgacagc atttattgca gcctttgtga cagttaatgt
    10021 ttacaaggtc tgtgtgaaaa ataatgttac tattcgtatg cctgaagagg ttccaccaaa
    10081 tatctctcaa gtatttaaag acttgattcc attcactgta gcggttgttc tcctttatgg
    10141 atttgaactc attgttaagg gaactcttgg tgtaaccgtt gcagaatcaa tcggtaccct
    10201 tcttgctcct ctcttctcag ctgcagatgg ttaccttgga attacactta tctttggtgc
    10261 ttatgccttc ttctggtttg ttggtatcca cggtccttca attgtcgaac cagcaatcgc
    10321 tgctatcact tatgccaata tcgatgccaa cttgcatctt atccaagctg gacaacatgc
    10381 agacaaagtt atcacttctg gtactcaaat gtttattgtt accatgggtg gaacaggagc
    10441 tacattgatt gttccattct tgttcatgtg gatttgtaaa tcagaacgta accgtgccat
    10501 cggacgtgcc tcagttgttc caacattctt tggggttaat gagccaatct tatttggtgc
    10561 accaatcgta ttgaatccga ttttctttgt accgttcatt ttcgctccga ttgtcaacgt
    10621 ttggatcttt aaattctttg ttgacacatt gaacatgaac tcattctctg ccaaccttcc
    10681 atgggttact cctggtccgt tgggaattgt actcggtact aacttccaag ttctatcatt
    10741 tatcttagcc ggactcttgg tagttgttga tactatcatt tattacccat ttgttaaggt
    10801 atacgatgaa caaattcttg aagaagaacg ttctggtaaa acaaacgatg ctcttaaaga
    10861 aaaagtagct gcaaacttca atactgctaa agcagatgcc gttcttggaa aagcaggcgt
    10921 tgctaaagaa gatgtagcag caaacaataa tatcacaaaa gaaactaatg tccttgttct
    10981 ttgtgcaggt ggaggtacaa gtggcttgct tgccaatgcc ttgaacaaag cagcagcaga
    11041 atacaatgtt ccagtcaaag cggcagcggg tagttacggt gctcaccgtg aaatgttgcc
    11101 agagtttgac ttggttatct tggctccaca ggtcgcttca aacttcgatg atatgaaggc
    11161 tgaaacggat aaattgggca ttaaacttgc aaaaactgaa ggagctcaat atatcaaatt
    11221 gacacgtgat ggacaaggtg ctcttgcatt tgttcaacaa cagtttgatt aataaatttg
    11281 taaaagttat ttgagacgag attattcttt caattcctta taatattgtt aaccatatca
    11341 ttgcatttca tttccttggt ttccggaact cagaatatcg tctcaaatgc tttttttgaa
    11401 aggatttata gttatgacta aaacacttcc taaagatttt atttttggtg gtgctacagc
    11461 tgcttatcaa gcagaaggtg cgactcacac ggatggtaaa ggcccagttg catgggataa
    11521 gtatcttgaa gacaattatt ggtacacagc agagcctgca agtgattttt accataaata
    11581 tcctgttgat ttagaattag ctgaaaaata tggcgttaat ggtattcgta tttcaattgc
    11641 ctggtctcgt attttcccaa ctggctatgg cgaagtaaat gaaaaaggtg ttgaattcta
    11701 ccataatctt tttgccgagt gccacaaacg tcatgttgag ccattcgtga ctttgcacca
    11761 ctttgacaca ccagaagttc ttcactcaga tggagatttc ttaaaccgcg aaaatataga
    11821 acactttgta gattatgccg ctttctgttt tgaagagttc ccagaagtaa actactggac
    11881 aactttcaat gaaattggcc caattggaga tggtcaatac ttggttggta aattccctcc
    11941 aggcattcaa tatgatcttg ccaaagtctt ccaatcacac cacaatatga tggtttctca
    12001 tgcacgtgct gtgaaattgt ataaagataa aggttataaa ggcgaaattg gtgtagtaca
    12061 tgcgttgcca accaaatacc cttatgatcc agaaaatcca gcagatgttc gtgccgctga
    12121 gttagaagat atcatccata acaaatttat cttggatgca acctaccttg gacactattc
    12181 agataaaacg atggaagggg tcaaccacat cctagctgaa aatggtggag aacttgatct
    12241 tcgtgatgaa gacttccaag cacttgaagc agctaaagat ttgaacgatt tccttggtat
    12301 caactactac atgagtgatt ggatgcaagc ctttgatggt gagactgaaa tcattcacaa
    12361 tggtaagggt gaaaaaggaa gttctaagta ccagattaaa ggtgttggtc gccgaatcgc
    12421 accagattat gtgcctagaa cggattggga ctggattatt tatcctgaag gcttgtatga
    12481 ccaaatcatg cgagtgaaaa atgattatcc aaactacaag aagatttaca tcactgaaaa
    12541 cggtcttgga tacaaagatg agtttgtaga taataccgtt tatgatgatg gtcgtattga
    12601 ttacgtgaag aaacacttgg aagttttatc agacgcaatt gcggatggtg caaatgttaa
    12661 aggttacttc atctggtcac ttatggatgt cttctcatgg tcaaacggat acgaaaaacg
    12721 ttatggacta ttctatgtag actttgacac gcaagaacgc tatcctaaga aatcagctca
    12781 ttggtataag aaagtagcag aaactcaagt gatagagtaa tattacttga aaatgtaaaa
    12841 aattgttatc cgatacttta gaaaaagtac aacacttatt tagaccaggc tttgaaaagg
    12901 tatatattgt tagtttcgaa gattgtccta tgattcctga gcttgaagct accccgttgc
    12961 taaaatgtgg taaatggtat gtttcaactg gaaaagagtg gatttgccat tctgatttag
    13021 agttatcagc atttgaatgg gaatttttac aatcactcga tgtagaaata cgtgagacta
    13081 ttcacttcga ggtaaattat ttgcctttcc aataataaaa agaaaggaat tagttgtgac
    13141 tatcgaatta aaaaacgagt atcttactgt tcagtttaaa acacttggtg gtcaactgac
    13201 ttcgattaaa gacaaggatg ggatagagta tctctggcaa gctgatccaa attattggaa
    13261 tggacaggcg cctattctat ttcctatctg tggtagttta cgaaatgatt gggctattta
    13321 tagacctcaa gaaagaccct tttttacagg tcttattcgt agacatggtt ttgttcgaaa
    13381 agaagaattt attctggaag aagtcaatga taatagcgtg acctttagta ttaaaccaaa
    13441 tgctgagatg cttgataatt acctatatca gtttgaactg agagttgttt ataccttgaa
    13501 tggtaaatca attagaaccg aatttcaggt aacgaacttg gaaatggaaa aaacaatgcc
    13561 ttactttatt ggagcccatc cagcattcaa tagtccttta gtagaaggtg aaaactatga
    13621 ggattattat attgaattta gtgaggttga gtcttgctcg ataccaaaga gttttcctga
    13681 gacaggatta ttagatttgc aagatcggac accttttttg gaaaatcaaa aaattctcga
    13741 tttggactac tcactttttt cccatgatgc cattacactt gaccagctca agtctcgtag
    13801 cgtaacgctt cggtcaagga agtctggtaa aggtcttcga gtagattttg atgattttcc
    13861 gaacttgatt ctgtggtcta caactaataa aagtccattc attgctttgg aaccttggag
    13921 tggtctctcc acttcacttg aagaaggtaa tttcttagaa gataaaagac aggtaacgaa
    13981 aatttcacct caagaaactt ctagaaaaat ctatgatatt actatcctta attaaaattt
    14041 gttattattt gagtcttcaa taagatttga atactcttac cttgtgatga tagattggtc
    14101 ttctccgtgc cagtcttaca tcgcaaggtt tttaagtttt ttgtgggtct atgtcaactg
    14161 tagtgggtga cgaaaagcta atatctaaag acagtctggt agtaaaaaat aagattattt
    14221 actacttctt gatttaaaat aatagtggag ccattaattt gtggcaaatt taatttaata
    14281 ttttgcctca aattagccac actttttata gaactaagag gaaaaatgaa attttcttcc
    14341 tattaatata gttgtttgaa atcttaaata tagcgttttg g
//
LOCUS       DAFEPQ010000065        14161 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.067, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000065
VERSION     DAFEPQ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.067"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            66..767
                     /locus_tag="LP543_002132"
     CDS             66..767
                     /locus_tag="LP543_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBL2322289.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="LP543_002133"
     CDS             764..1885
                     /locus_tag="LP543_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBL2322290.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="LP543_002134"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="LP543_002134"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBL2322291.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="LP543_002135"
     CDS             3264..4172
                     /locus_tag="LP543_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBL2322292.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="LP543_002136"
     CDS             4207..4479
                     /locus_tag="LP543_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBL2322293.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="LP543_002137"
     CDS             4490..5536
                     /locus_tag="LP543_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBL2322294.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="LP543_002138"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="LP543_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBL2322295.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9233)
                     /locus_tag="LP543_002139"
     CDS             complement(8547..9233)
                     /locus_tag="LP543_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322296.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(9211..9705)
                     /locus_tag="LP543_002140"
     CDS             complement(9211..9705)
                     /locus_tag="LP543_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBL2322297.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="LP543_002141"
     CDS             complement(9725..10945)
                     /locus_tag="LP543_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2322298.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="LP543_002142"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="LP543_002142"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBL2322299.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(13021..14148)
                     /locus_tag="LP543_002143"
     CDS             complement(13021..14148)
                     /locus_tag="LP543_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL2322300.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         4574 a         2603 c         2928 g         4056 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatctt ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaaccatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaaccta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc cagtagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggacgagg acgaaccgaa gaaatagcaa gggtttgttt gaaaacagtg taaaaaataa
    12961 accatgttta catggatttg ccaccggttt gccaccggtg gtttttttcg gtggcaaaaa
    13021 tcaagaattc tttctaagat tttccatgat atcgaccgtt tcatttctca tcttatctgt
    13081 tacatgcgag taagtatcca tcgtaattga aattctgctg tggcccaatc gttcagagat
    13141 ttctttcatt tttgcgccat tttcgagaag aagtgtagca tgagtatgtc tgagagaatg
    13201 gaagttaaaa gagagggaga gtgcattcga tatcctccta gtattccatt tcactacact
    13261 tggcgtaact agctcaccgt cttccttcgt acatactgca tttgaatcaa tgtagagctt
    13321 tccatacttc attcgatttt ctaattgttg tttcttatgt tttttcagaa ttgctagcaa
    13381 ggtttgtcca ataaaaatcg ttcgattgga actgcttgtc tttggcgtac catataccca
    13441 tgcgccgtca ttctttacca tttgtttctc tacagtaatt gttccatttg aaaaatcgac
    13501 atcatcccac gtcagaccac aaacttcgcc aacgcgcatt cccgtataaa atccaatatt
    13561 caaaggaata tagaaaggat gtccttcagg agtaatttct agcatatgat caaagtcctc
    13621 aagagaaatg atttttagat cttttttagt cgttggtcgt gcttcatatt ttggtatttt
    13681 tacatacagc ataggatttt gcttgattaa tccccaagga taaaccgcca tattcagcgc
    13741 attcttaagg acagagtgag taatagtcat tgttttcttc gagtaaccct ttttaaattc
    13801 agcattgatg aaattttgta aaagagcagg ggagagatcc gtaagttttt tctttcctaa
    13861 ataaccgttt atatgatttt tgatggtaaa tcggtagttt tcataggtat tgtattttag
    13921 atttagttta acgtattcct ccatccaaaa atcaaggtat tgttttactc gagtatccgt
    13981 acctaaaaag tattgtcctg tttcgtcaat atctgataaa acttttcgta aagcagcttc
    14041 ggcctctggt cgggtgtctc cgccaacttt ctccactttt tttcttgagc catcatcatt
    14101 gatatcttcg ccgtttgtat aattaagcga gacaaataaa aaagccattt atgttacact
    14161 c
//
LOCUS       DAFEPQ010000066        14032 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.068, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000066
VERSION     DAFEPQ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.068"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..1350
                     /locus_tag="LP543_002144"
     CDS             <1..1350
                     /locus_tag="LP543_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL2322301.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..2827
                     /locus_tag="LP543_002145"
     CDS             1484..2827
                     /locus_tag="LP543_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL2322302.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2939..4390
                     /locus_tag="LP543_002146"
     CDS             2939..4390
                     /locus_tag="LP543_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBL2322303.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            4739..5371
                     /locus_tag="LP543_002147"
     CDS             4739..5371
                     /locus_tag="LP543_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HBL2322304.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            5959..7398
                     /locus_tag="LP543_002148"
     CDS             5959..7398
                     /locus_tag="LP543_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2322305.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            7693..7911
                     /locus_tag="LP543_002149"
                     /pseudo
     CDS             7693..7911
                     /locus_tag="LP543_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8160..8375
                     /locus_tag="LP543_002150"
     CDS             8160..8375
                     /locus_tag="LP543_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322306.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8592..8848)
                     /locus_tag="LP543_002151"
                     /pseudo
     CDS             complement(8592..8848)
                     /locus_tag="LP543_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9035..9946)
                     /locus_tag="LP543_002152"
     CDS             complement(9035..9946)
                     /locus_tag="LP543_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL2322307.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(9965..10969)
                     /locus_tag="LP543_002153"
     CDS             complement(9965..10969)
                     /locus_tag="LP543_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBL2322308.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(10966..13089)
                     /locus_tag="LP543_002154"
     CDS             complement(10966..13089)
                     /locus_tag="LP543_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL2322309.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(13094..14014)
                     /locus_tag="LP543_002155"
     CDS             complement(13094..14014)
                     /locus_tag="LP543_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL2322310.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         4579 a         2498 c         2634 g         4321 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg ggaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2641 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     2701 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     2761 tcctagtttt agccaaccct ataattttat tttaaaggca gtaacaggat taaacattga
     2821 aacataatcg ttactaaatg aaatcaagaa atatactgta ttactcaatt cattgaaaat
     2881 atttatcata atttttaata gaaaacacta ttaaagagaa aaaagagagg taaatcaaat
     2941 gaaaaaatct ttaaaacttg catcagcagt tatattaatg tcccaactcc ttatagcaac
     3001 acctttccat gtgttggcaa gcgaaaattc aacatacaaa attgcaaaag aaagaaccga
     3061 gacgactgaa atgagtaatt ccgatgtaac agcagagagt agtgaacttt tgacaacttc
     3121 atcaagtgaa agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag
     3181 tagtacgact tcaaatgccg agtcttcaac aacttcatca agtgaaagta gtacgacttc
     3241 aaatgccgag tcttcaacaa cttcgtcaag tgaaagtagt acgatttcaa gtaccgaatc
     3301 ttcaacaact tcaagtagta caagtgaaat aacttcttcg aaagtgacaa aaccaaacca
     3361 tatagaatct tcaaaaaagg aagcgccaaa aacggaagga caaacaaaaa ctttttcgac
     3421 accacagcaa atagaatcaa cagaatcttt ccaatcacaa ccaatactat ctggtaatga
     3481 agaaattcat tttgacaaaa atgaatctgt agaaaatttt attatgaaaa ttggtgagtc
     3541 tgcgagaaaa attggtcaga aatatgattt atatgcttct gttatgattg cacaagcgat
     3601 cttagaatct gccagtggtc aaagccagtt agctcaggca ccgaattaca atttatttgg
     3661 gataaaagga acttataatg gaaactttgt cattatggta acaaacgaag atttgggtaa
     3721 tggaacgctt tatacgaccc aatcaaaatt tagagtgtat gaaaactatg aggaaagctt
     3781 tgaagattat gccaaattat taactaaggg aattagtgga aataaagatt tttatgcagg
     3841 agcattaaaa gctaatagta aaacatatcg agaagcaact aaattcctta caggacggta
     3901 tgcaacagat acacaatact atttgaagct aaatgaatta attaaaacct acgatttaac
     3961 caactatgat aaagaagtta aaggtccaca aatgagtaag actggatacc aagtgcctat
     4021 tcataattat tcaataacca gtttgtttgg aaatcgtgga aatgaatttc accgaggact
     4081 tgatatggca gctacacagg gtgagccgat tcatgcagtt aaagctggta aggttgttaa
     4141 agcagagttt cactactctt ggggaaatta cgtggtgata caacacgaag atggatcaac
     4201 cgcgttatat gctcaccagc aacaatacct agtaaaaact ggagataatg ttttgcaagg
     4261 acaaataatt ggctatgtag gaagtactgg gaatagtact ggcccacacc ttcatttgga
     4321 aatttgcaaa gatagtagtc tttctcagag catgttagtt gatccgaaaa ccttattatt
     4381 cggagactaa aaaaggtaat caatctgaca aagtaacaag aaaatggttg gattcttaga
     4441 cctttgatca ggatattgag actggaattg atatctcgat tacgatgggt ttgacaaata
     4501 aaacaagtcc attatcgaat atcgagtgta ttattgccgt ctttatgctc atattttgag
     4561 caggtttcac tagatgaaaa caatttatct atcttgagga tttcgtgtac gtatcgttca
     4621 gtcttgtatt gtaaattagt cataaaatca gactaagaaa aattggaaat atttttttct
     4681 gatttattaa aagttaattg aaattatatg gtacttcttt cttgggaggg taggtgcagt
     4741 gaaggaaaga tatggtcacc ttactgttgt tgaggagatt actcataatg gatataaaaa
     4801 atatcgttgc ctatgtgact gtgggaatga aacgattgta tatcgatcaa acttactatc
     4861 aggtagaacc aaaagttgtg gatgtggaaa ttataaaaat aggcataaat ataaaaattt
     4921 gacaaataaa acttttggta agttagttgc aatcaagcca actaaactgc gaagtgaagg
     4981 aactgttgtt tgggaatgtc aatgtctgtg tgggagaaaa aagttagtat cggcaaagcg
     5041 acttctaaaa ggtgatgtaa aaagctgtgg atgtttgaaa tcaaaaaagc atcacattga
     5101 aaataaaaaa ttcggattac ttactgcaaa gtatcctttg gacaataact gttcatggaa
     5161 aacaaaatgg gtttgtcatt gtgagtgtgg cagtatgtgt gagataagtt atagtaactt
     5221 attttttgga catactcaaa gttgtggatg tttaaaaagg caagaaacaa ggacatgtgt
     5281 tgaagggaca gtaatagagt gtctaacgtc aaaattaccg aaaaataata caagtggtgt
     5341 taagggagtt tatcgttcta aagagaaata gattgcgtat ataactttaa aaaaaaagag
     5401 gtactattta ggatcgtatg attctattaa gagtgcagct caagctagaa aagatgctga
     5461 aaataaattg tttcaacctt tgattgacaa atatcgaggt aatcattaat tattgtggat
     5521 aataattaac agtgatatat aaacatctat aatatattat tcatactatt gatatttagt
     5581 ataatcctat ggaattgaat atcatagcaa tgtatttgaa aagcaaattg gaaaacaaat
     5641 aggtgcaaca ccataaatat gaaaaaattg ggaaaaagta gaatggaaat ttgcttacgt
     5701 ctcacgagat agctcacact attaatctga agaacaatga agtcgacttt tagaaattgc
     5761 tagaagaaag taaagcgaat tatcactagt tactatagag taagttcaaa taaagcatct
     5821 ccaacttaca tggttacatg aggatatcaa atcgaaatta ctcaagcttc ctatggctag
     5881 agtcgcgaga agttgttggg cagtagacat aggttatatt gttcttagct acacgatcat
     5941 tagaaaggaa atcctattat gtatagttat caagaagatg caaaagtcca aaaaaatcgc
     6001 tggtggattt tgattgctat atctatgttt accttcatgt ccacattgga tggcagtatt
     6061 gtcaatattg cactacctac catctcagat gatatgggca taccaatgaa tcaagcggaa
     6121 tgggtagtgt ccgtttatct gatgattgtc tgttcttgtt tgttgctgtt tggtaaaatt
     6181 ggcgatagcc gtgggaaaat caaagtattt cggattggta cgctgatctt tgtcttggga
     6241 tcgctgttgt gtgggttcaa tcattcgcta ggtattttat tgttcgcacg ggtggtacaa
     6301 gccattggtg ctagtatgac tatggctaca aatgccggaa tcatcacgga agtctttccc
     6361 atacatgaac gtggtcgggc attgggagca ataggtgcgt ttgtttcatt aggctcgatt
     6421 gctggaccag gtcttggcgg cttgattttg tcccaatttt cttgggcgta cattttttgg
     6481 atcaatgtac cggtgggaat cgtaacgatg ttgattgctg aaaaattctt accaaaagat
     6541 atcaccatga gcggccatac aatcgatgga gggggctttg ctgccattgc ggctacgatt
     6601 atcagctttt ttggagcaat ctttttgggg caagaaagtg gctttttcca tccagtcccc
     6661 cttgtactgt tcgtaatttc agtgattgcc tttgtcagct ttttaaaagt tgaaaagaaa
     6721 gcagcctctc cattgatttc tttcggtatt tttaagaaca agatttttac catgagcttg
     6781 attacagcct tgttgatttt ttcttccaac ttctttgtca atgtagtgat tcctttttat
     6841 ctgcaaaatg ctcgcgggtt gagtcctagc tatgctgggc tgttgatgat ggtctttcct
     6901 ttgatgatgg tcgtcggggc gcctttaagc ggctacctga ccgaccgggt tggcccggag
     6961 attttggtat tagtaggctt gggcttattg tcggtaaccc aggtcatgta tatgtttttg
     7021 tcagtggact ctcctttgtg gctatacatt gtcgctactg ccatcatggg tgccggcaat
     7081 gccttgttcc agtcccccaa taatacgatg gttatgagct cggtcactat cgaaaacttg
     7141 ggactggcag gctcgatgaa ttcctttgcc cgaaatttgg ggatggtcag cgggatttcg
     7201 ctttctacga cggtcttata tcaagcgatg tctgctaaga tgggccagca agtaactaac
     7261 tatatcgcaa atcggccgga tgtcttttta tatggaatga agattacctt tttaggcagc
     7321 tttgttttgt gttttgttgc ctttttcctg actatctggc gtatcaaaaa acaaggaggt
     7381 attcagaaaa atgtctgaaa aaaccactgt tgaagaattt ttggaggcct gcgtatgggt
     7441 gagtgggatt tccatggatg atggaagatg aagtgaactt ctaaaagccg aaaaactcaa
     7501 gtattatctt caaaatacta gtgctcaatg agatgcttga tttgatcaca agaagactgc
     7561 gaaagccgct ggcaaccgca agctaggtat cccaccgatg gactacgttt tagaggctac
     7621 tggttatgaa tacggagcca acgctctcat ctgcataaga atacatcacc cagattatgt
     7681 aaagattttc gatacttctg tcaaaaaatc attaagatag ataaatgatt tccgtctagt
     7741 caaattggtt cagaatgtag acacacagat ggtaagaagt cactcacaat tcgagaatgg
     7801 acttgtccta tttgccgtat acatcataat cgaaacgtca acgatagtat caatattttg
     7861 accaagggct atgaatacaa gcattgcctt agaaaacatc aagaacctta ggaacaacgg
     7921 ggatagcttg gttaataaga gataccgctg tttaggcttg caagtatact cttttatcaa
     7981 gaattccgtg gctttagtca tgagaggctt aatactatga aaattttctt tagcattttt
     8041 gatttaggtt ccttttctaa ttataataag atgaccacta cagtaaccta ctgaaatata
     8101 taaacagcca ctgtagtacc attttcaaag tagtctatga gaggagaatc ttcattctta
     8161 tgaacgctgg aattgcggat atgaatttaa taaaaaaaac ccttaatgat tttacttcca
     8221 attcaatatc aaaaggtact ggtatcaatt taagcacaat aaaaaagctg aaaagtggtg
     8281 aaagatcagt ggaaaagctg aatcttttag atgctataaa aataacggaa tttgctatga
     8341 aaaatggaaa agctgagatt gaaatatgga gataattatc gtctagtgaa ttacttgcca
     8401 cttagctgaa tttaacgatc cttggtgctt gaagggggag attcttggga atagagcata
     8461 cttgcaagcc tatgtcttta ctaacaacga tatctcttat taaccaagct atccccgtag
     8521 ttctaaggct tttactttta tctaagcaat aatcgtattc aaaggaaatt tttgaccctt
     8581 agaaagtctc attttatcag acgtgagctt aggcattgct gaattttgtt aattcggttt
     8641 taggttgtgc tttgagcata atatgaaagt attctttatc acgatttcat tcatctaagg
     8701 taatgtgatg tgattctgaa attctttcaa aggcagtttt tgcataagct gacattacat
     8761 catcaatgac atgtttacaa tgtttcacta ctaaaataag ggggtaacca agaagaaaaa
     8821 ctgaatgatt atttgtatct aattccatgg tatatcaacc atttcctata tagactgatt
     8881 atgacatgga ttaaaataaa aaggcactat gtcttaattg tcggttttcg agttaccgaa
     8941 tggcattcat cacccaccta tagaggatgg gcgacttctg cctgaaataa gttacactac
     9001 ttataacttc taataaaatt agattgagtc attattattt aacatcaacg attttccagt
     9061 ttccattttt ttgtaatact aatttgtact gaaagtagct agtggttttt gatacattat
     9121 caaggtattt tatctttgta ctcactagaa aattttttcc gttttgtaga taaactggat
     9181 tctctaactc cgaaaacttt aagtcaatat tgattggtcg catcgcacca ttttcaacat
     9241 aatactccaa ttctttttca gaagctgatg gataaagttt aaaaaatgtt tctaaaaaat
     9301 cggtgatttc ttccgttgtt ttggcatcaa tttctgaatc gttttctact tgttttgctt
     9361 catattttgc cttgtctggc tttccaatca ttgttggcaa actagttact aaatgattcc
     9421 tgttttcatc ttcaaagatt gtcactcgat atgcagaaga aattgattgc gtcttttttc
     9481 cctctgtaat atgttgctcc actaaaaatg aaacgacaaa ttcttccgag tgtttttcaa
     9541 catctagaat ttttacagat tgaacttcgg aactcgtggg aatatctgct cgaaccatat
     9601 cttggattaa agcgagaccg ttttctgtaa ggtactgttc gagattactc attctctttt
     9661 caattgcttc tttgttattt ttccatgaaa aatattcttt cgcaaaatcc tctgtaaaac
     9721 tttcaacgcc agaagtattc actaccttct cttttataac cactttttca tgagttgtat
     9781 gttgatcaat ggcggtgaaa tttttataaa cgccaaaagc aagactacaa gacaataaaa
     9841 gccaaagggc aagcaccatt tttcgatgct tacccacgga tacaactcgt tcttttttct
     9901 tttttgggct cttttccttt tgattccgtt caatttttat cttcattctt tttcctcttt
     9961 tctcttattt gtatcggcca gctccgataa gatgctgccg ccaatagttt tccgttagat
    10021 tcgtccaccc cactggatct cctgcatgaa acatgcgatt ctcccctgcg taaatgccca
    10081 catgggtcac ataagtaccg gcatcatagg ttgaatgaaa gaaaactaaa tcacctggtt
    10141 tggcatcttt tagatcaata tgagtcatgg catcatattg atcctgagcc acccgaggaa
    10201 gtttcaggtc aactttggca taacaccatt gggtcaagcc actacaatca aaccccgttt
    10261 ctggacttga gccaccaaac acataggccg ttccttcata cttaaatgcc tcatcaaaaa
    10321 tttcttggac tattttgtca tcaaactgag ttgtagttag gtattgcttc actaataaac
    10381 agtaaaacat attgccataa ttataccgcc acccaccatt ttccttaatc gcgattgggt
    10441 tggggtaatc gactttttga ccacctgatt tttcctttga aaaagattca gctaaagcaa
    10501 aactatgctt cttcccgttt ttcccgacat gatctagata cccgccacca tagttatagg
    10561 cctgaataat actatcttga tcacacccta gttctttgga acgcttaacc aaagacgcaa
    10621 aatagagaca gccttgtttg atggatgcct cggtacttaa tgaattaggt ggtaatccgg
    10681 ctgactcaga actttgcata acgtcttcca ttgtgccacc agattccact tgaataatcg
    10741 ccaaaagaat tggaatatac tctgttatat ccgattcttt ggcatacttt tccaccattg
    10801 cacggtgttt caatacttct tctgagacac tagttccgcc aggagaagtg ccagaactac
    10861 catttccctc actgtcttca ccaaacaaca aacccacaca catcaaaata cccataaaag
    10921 agatggtcag aaaagttaat gaaataacac ccactttctt caagttcatt ttttctcacc
    10981 tgaccttgat tttggacgat ggttcattct tttttgtact ttttgattgg tctgctttgg
    11041 cagagtggac ctctgtttca cttgttggaa agaatttttc ttacttgctg attggctttt
    11101 cccttttgaa atgatttctt gactagttag ttttcgtttg atttcgggat tcagatttgc
    11161 tctcacaatc cgctttggtg cttctttttt cagcatcata tctttctgag gttgagctgc
    11221 tttatctatt ggaatattcg cccttctttt tccctgacga tttgttgctt cactcagtat
    11281 cttgcgtttt tcgtccattt tcgcatttct tcgttgtcgt tgtttttctc gcaaatccac
    11341 tcgatccgct ttttcttgga acagtccgcg cttgaattct tctggtgctt ttttcgactt
    11401 ttcgattcct ttatggagat tgtatttcaa attcgttggc gtattcctta cctgatcctt
    11461 tacttgttta gtcttatctt tcaccaaagc ttgggtatcc aaaactttgc cgatttttct
    11521 cccagccaaa ttcattcggg attgtttctt attatttgaa actggattct ccttagttaa
    11581 gtcagatagt atttcgtgat cgttcggtaa tcgtgaattc tttcgtagag aagaaccgga
    11641 tggcttaaat ttggattttg attgttcctt tgccaactta tttccgactg ctgcaccacc
    11701 accaaaagcc aaagctcgtc ccacatttcg attgagttgc cgcaattttc gattcattaa
    11761 catttgcggt tttcgcatga cgcgtcttcc cattgactgg ctatcattac tctggaggtt
    11821 gaacatactc ataatgtcgc ccaatttgaa atagataccg gcaaaggtca caatttgcag
    11881 aaaagcaacc atgaaaaatg gataggttgc cgaaatattg aaaaacatcg tggagatgct
    11941 aaatgcagtc gtaatcacca aagtaacacc cgctcgcatc ataatcgtat tgaataaacg
    12001 aataatcgcc ttctttccta aactttcata agtcggcaac atggacaata agaaactaat
    12061 tggcagaaac atcgcaaaga taatgaagag aatttgcgag aacaacataa tccctgttag
    12121 aagaaaaaca aagaaggaaa tgccaatatt gaacaatacc aaaaagacca cggtccctaa
    12181 acggttcatg gtcttggtaa tactgagatt cgcattgtca ttgtcttcaa tctctgcctt
    12241 gaccgcttct tcccgatctt tgcctttgtt ttcatctgga ctcacagata gaatcttgtt
    12301 gacccggtct tcaccaattt cttctatgtt ggaatcatca aattgcagta gtaaccaagg
    12361 ttgttggact tgaatcgaaa acaaactatc ccgaatcaaa tccacactat cttttccttg
    12421 gctttctgaa ttgggtacaa cgatctcggt tccaagggta agggccgctt cactaacatc
    12481 tgagctaaag tcattgattt ttgtgatata ggtaggcgca taagcaatga acgaacccga
    12541 tagtagaaaa atcatcacaa aattaatcac ggctcgaact gctttcgtgg tttctcgttt
    12601 taataatccg gtatacgcca catacacccc cagagctaaa atcatcaata atagaaaccc
    12661 aaaataaaat ccggaggtct ggagtccatt ttcagtaata ccagccaatg tttggatatt
    12721 ctttccaata ctttcagccg tatccgaaat gaaatctaat ttgtaagcct cttgaaccac
    12781 ataacccgtg gcgttcgaca aatacaaact caccgtccaa atgaaattag taatggcata
    12841 aagaccatac atcacgcttt tccctaagcc atcaccccaa ttccaaggca accaatccca
    12901 agagctatcc acaaagaaat ctagttgata gttgttcagt gaatatttgg aatatagatt
    12961 tcctgcttca actgtatcat caactagccc tgtcgcctcg gctaccgtac caactaaact
    13021 caatagaact atcagagtga gtatgctaat aaaaacgatt cccacaatcc gaaaaagttt
    13081 ctttttcatc ccctcactcc attctctctt gaacaggtgg acgggtatca aaggcatgga
    13141 agagttcttc aaagactgga tgtacctgaa ccacgcctac ccgaccatac aaatcctgca
    13201 tcaagcattg gccattttcc aaatctcgta ggcgtttttg attgctttca tcttctcgat
    13261 ccaagccaaa gaattccaag gttttcttga tttcatgaat atcggtactg cgaaacgcaa
    13321 acttcaaccc gatattattc ttcaagcgtt catccagtaa atcatcacta ttttgcgtga
    13381 caaagtaaac cccggcattc atagcccgcc cagcacgaac gagcttattc gataatgttt
    13441 tcccttgcgc tacttgaaga aaactccacg cctcatctaa gtctacaatt ttgaagattt
    13501 ctcgattact atggataaag tccaaggcaa aagtggaaat gacgattagc atggaaacac
    13561 taagcaattc catggtggta tattcttcaa aagtggtttc tgcatctggt aaaaccaaat
    13621 ccgctacttg aatgatattt aactgcctat ctaaactaat cgactgggta atcgaaccat
    13681 ctgagaaaag caaatgggca aaatcataat cggtaaagga ctcgatgtga tccgcgatat
    13741 tttccgcaac tggtgagcct tcttttcgaa gctcattaat tacttgtaat aatccccgtt
    13801 gattgctttg ggtaaccgtc cgaatggcct tacgcaacac tggaaacttc tccccatctc
    13861 gactagaaat tcctgttaaa aaggtcagaa tatcaattgc tagactttca gaatccttct
    13921 ttttctccat aatcacataa ggatcaagta atccttgatt ttttggctta ctagttaagt
    13981 tgagaatatt gatttcatcc gcaatttctg gcaaggtttc tttccagcca cc
//
LOCUS       DAFEPQ010000067        13977 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.069, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000067
VERSION     DAFEPQ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.069"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="LP543_002156"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="LP543_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBL2322311.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="LP543_002157"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="LP543_002157"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBL2322312.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="LP543_002158"
     CDS             complement(2169..2906)
                     /locus_tag="LP543_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBL2322313.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="LP543_002159"
     CDS             complement(2997..3524)
                     /locus_tag="LP543_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322314.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="LP543_002160"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="LP543_002160"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBL2322315.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="LP543_002161"
     CDS             complement(4401..4886)
                     /locus_tag="LP543_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBL2322316.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="LP543_002162"
     CDS             complement(5039..5236)
                     /locus_tag="LP543_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322317.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="LP543_002163"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="LP543_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBL2322318.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="LP543_002164"
                     /pseudo
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="LP543_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
     gene            9211..9423
                     /locus_tag="LP543_002165"
     CDS             9211..9423
                     /locus_tag="LP543_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBL2322319.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="LP543_002166"
     CDS             9425..11104
                     /locus_tag="LP543_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBL2322320.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="LP543_002167"
     CDS             complement(11358..11558)
                     /locus_tag="LP543_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL2322321.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="LP543_002168"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="LP543_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(12249..12322)
                     /locus_tag="LP543_002169"
     tRNA            complement(12249..12322)
                     /locus_tag="LP543_002169"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12286..12288),aa:Ile,seq:cat)
     gene            complement(12634..13032)
                     /locus_tag="LP543_002170"
     CDS             complement(12634..13032)
                     /locus_tag="LP543_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322322.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
     gene            complement(13025..13477)
                     /locus_tag="LP543_002171"
     CDS             complement(13025..13477)
                     /locus_tag="LP543_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322323.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            complement(13464..13970)
                     /locus_tag="LP543_002172"
     CDS             complement(13464..13970)
                     /locus_tag="LP543_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322324.1"
                     /translation="MDIKWLVQQNDSNLELAIKYLEETIFEDEHLTDNFLQVLKYLEI
                     YSVKKNKLIGENDSPIKTPIELSLRNRMGILQRSEIVKELFYHKFSYEIRLDDTYEHY
                     RIVFFVYNSIEDATATTALTFGFTKNGTINSDKTRQAATESDDICKKVCNGEENYWIG
                     EEKLNEIY"
BASE COUNT         4117 a         2943 c         2347 g         4570 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttatcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgacatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaattatg taccctgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc
    12301 tgaccaactg agctaagagt acaggttgtt gccacataaa gccataaaca atcaaccagt
    12361 agaaggtgtg gcaacaatcc tgttatcgca tagcttggag tgtgactatt atgggtgata
    12421 gtgaagatat gcgataacat cactatttta tcgaatgatt tttatagttg tcaatacagt
    12481 catctaaata cttcatatca atcatttatt cagaaacaaa ctatcttgca ggatctatac
    12541 gagttaatta cagagcaaaa aagcctcaac caaggatgac tggttgaggt ctttgtcttg
    12601 caaatttaaa tagataactc tattacttgt attttatttt tgttgactat atagctgata
    12661 taaatcaata actgtaggca taactcctac ttcgctatat aaattagaaa tcaaattaga
    12721 agctttattc aggataggtt tagctagctc tcccatagat tcttctacta attttttata
    12781 tggctgttca tttgtagaaa cacgaataac tgaaaagagt aaaatatcta ccggtgctcc
    12841 ttccatcccc cctacaatgt gagctcgtaa agaaacaagt tttttttcta gatactctgc
    12901 ttcgtcttct ttatatccaa gttcaatatt aaactctaaa tctccataac taaaattatc
    12961 agttaattca aaatgaattt tattcaaact aatattttca agtgcaaaag taacactatt
    13021 tcctttatcc atgtacatat acctcttttt ctacaatata gcggctcgta tcttggtaat
    13081 taacactagc atagaccata tttgacgata cttcttttct ctttctactt ttaaatactg
    13141 agtaactttt attccgaata taaggtttat tttcaatacg gtcatattta atagggccgc
    13201 cagcaacctt aatagtgaaa ttcattgaat ctttatagga caaattttca acttgtttca
    13261 aaagtttatt gtactctgca acaggaatat gcatatctcc gaactcaaat tcaagatatt
    13321 ttttttcact tattcctaac atttttgaga attctgtcac gtttttttgt ttttttaatc
    13381 ttattttaag taattgtttg gacaggtttc tttgttcaga atttaatttt ttatataaat
    13441 caggatgttt ttttaataaa tcattaatag atttcattca acttttcctc ccctatccag
    13501 taattctctt ccccattgca tactttctta catatatcat cactttctgt agcagcttga
    13561 cgggttttat ccgaattgat tgttccattt ttcgtaaaac cgaatgttaa tgcagtagtc
    13621 gcagtagcat cttctataga attgtatacg aaaaaaacaa ttctataatg ctcataagta
    13681 tcatctagtc ttatttcata actaaattta tgataaaaaa gttcttttac tatttccgat
    13741 ctttggagta ttcccatccg atttcttaag cttagctcaa taggtgtttt tattggagaa
    13801 tcattttcac caattaattt atttttttta acagagtaaa tttcaagata ttttaatact
    13861 tgtaaaaaat tatctgtaag atgttcatct tcaaagatag tttcttcgag atatttaatc
    13921 gccaattcta aattgctatc attttgttga accaaccact taatgtccat ctcacta
//
LOCUS       DAFEPQ010000068        13837 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.070, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000068
VERSION     DAFEPQ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.070"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(24..1043)
                     /locus_tag="LP543_002173"
     CDS             complement(24..1043)
                     /locus_tag="LP543_002173"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBL2322325.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(1676..2680)
                     /locus_tag="LP543_002174"
     CDS             complement(1676..2680)
                     /locus_tag="LP543_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL2322326.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(2693..3742)
                     /locus_tag="LP543_002175"
     CDS             complement(2693..3742)
                     /locus_tag="LP543_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL2322327.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(3759..4580)
                     /locus_tag="LP543_002176"
     CDS             complement(3759..4580)
                     /locus_tag="LP543_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL2322328.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(4567..5346)
                     /locus_tag="LP543_002177"
     CDS             complement(4567..5346)
                     /locus_tag="LP543_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2322329.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(5363..5833)
                     /locus_tag="LP543_002178"
     CDS             complement(5363..5833)
                     /locus_tag="LP543_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2322330.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(5845..6264)
                     /locus_tag="LP543_002179"
     CDS             complement(5845..6264)
                     /locus_tag="LP543_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBL2322331.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(6345..9122)
                     /locus_tag="LP543_002180"
     CDS             complement(6345..9122)
                     /locus_tag="LP543_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL2322332.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            9313..9537
                     /locus_tag="LP543_002181"
     CDS             9313..9537
                     /locus_tag="LP543_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322333.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            10316..12031
                     /locus_tag="LP543_002182"
     CDS             10316..12031
                     /locus_tag="LP543_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2322334.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            12034..13797
                     /locus_tag="LP543_002183"
     CDS             12034..13797
                     /locus_tag="LP543_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2322335.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         4065 a         2831 c         2402 g         4539 t
ORIGIN      
        1 acgattaact attgatcatc tatttaacga agagaaaatt cattctccat ttttccgtca
       61 cgtaacatga tttcttttgc tgcttttttg atatcttctc cattatacaa aacattatag
      121 attgtttgag taattggcat ctcaacacct agctgttgag ctagctctac tgctgctttt
      181 gtcgttgcca caccctcaac gaccattccc atgttttcta gaacttcttc cagcttatgt
      241 ccctgtccta acaagttacc ggcacgccaa ttgcgagaat gaacacttgt acaagtaacg
      301 atcaagtccc ccacaccact caaaccgata aatgtcagag gattggcacc catcgcgaca
      361 ccaagacggc tgatttctgc caatcctcga gtcatgattg ctgcttttgc atcatctccg
      421 aatcctaatc catggatagc gccggcacca attgcaatga tatttttcag tgctgcacca
      481 gtttctacgc caatcacatc attatttgta taaatcctga aataatcatt catgaacagt
      541 ttttgtacat aggctgctgc tttttgatca atacttgcag ctgtaattgt tgtgatatca
      601 tgtactgcca cttcctcagc atggctagga ccagataata caacaattgc ctgtcttttt
      661 tcttctggaa tttcttcagc caaaacttct gagattcgtt tatgcgtatc ttgttctagt
      721 cctttacttg catgtataat gattggctta gtattcattt taccaataag ttcctgtgcg
      781 actgaacgaa tcgcttttgt tggaacaaca aataaaatag catctgcgtt atccactgct
      841 tctgccaatt ctttatatcc gataatcgat tctgggatcg tcaaatcagg taaaaaatgc
      901 tgatttgtat gatacttatt gatttcatca atctgttcag gtacatttcc ccaaatacgg
      961 acatcatgcc cgttttctgc caatgtttgt gctaaagcag tgccccaaga tccaggacca
     1021 agaactgcga ttttttgttt catggatatt cctccaaata aataattgat aatttccatt
     1081 atagtataca tttcttaaag ttgggactaa tttccattgt ttttaagtgt ttgtttactt
     1141 cttttatcgt ttttacttga aataatcaat gatatgttac aatagtctac attttatttt
     1201 tacactcgtt atgctttgct tgttcctaag aggaagcagt tttgacaatg ttactaccag
     1261 cattactatt aggaggttag gaaacttctc ctagtgagta ttgatttaaa atacataaga
     1321 gacagattga acatttctaa atctgtcagg tcctgcaacg gacagattgt ctctgttgac
     1381 ggttgacata ggaacttttg ttttagtcaa cattctggat acaaaacgct atgtaaactg
     1441 gcgcaactcg ttgtacttgg acttttacta cggatccatg caacaactaa atagtactat
     1501 tgcataacaa aaaatggttg gcacccacat gtcccattaa acagattata agttgaactg
     1561 tagataatgc aaaaaatgtg ttttataacg taattgatgc caatatttta aaaaaataaa
     1621 gggagcagtt atcgctgctc tctttatttt ttatattctt tttatccagt ctatcctaat
     1681 aatcaaactg acgatagtag cggcgtatct ctaatggatg gcagttcaac tgctccacat
     1741 gagcatcgat gcgttgtgtc actacatgca tcaataagaa agacaaaaca cgtccgcgat
     1801 atttttcact gataccttct actggatagt cttttgtgtc tatcaatgta taattgctgc
     1861 aaatttttgg caaaagttta gccacacgtt cagataacac tctctgttca tcttcgccta
     1921 aaaataatgt gactggggtt gtttcttcta cgatctctaa cgtcccgtga agaaactcgg
     1981 cagcatggat agatttcgat ggaagccagc tttgttcttc ccagtaacac atggcatatg
     2041 aataaacagc tccccattga ttccctgctc caataaaata atgcatacta tcacgtcgat
     2101 gtttttctgc aaatgataag ccaaatgcat ctgctttttt ctctgcttcc actaaccctg
     2161 ttggtaaata acgttcgagc tgttcatagt attctgaata agcttcgaat tctccattca
     2221 aatacatcaa tcgatctgct gccataaaaa atttgatttg ttctgttcca ggagctggat
     2281 aagtgaccac aaaatcgcat agctgagaca acttactgtt cgctttatcg ataaatccaa
     2341 tcagtgtcgc tcctactttc ttgatttgtt ctactgcttt cactacttct tgagttgtac
     2401 cggtaacaga ggacaaaata acgatagaat cttttgttaa tcttttgttt ccagtagtgt
     2461 aatattctgc agcatgctgt acaaagacag gcaagttgct tttcccattc atataagtga
     2521 cagcttgcat cgaggaagca tacgttccac cgattcctag ataatagaca gcgtcaaatt
     2581 tttccgcata aagtttatcg atgatttctt ctacttgcgg acgtaaagct agtgcgcctt
     2641 ttattgcttt gatttgttct tcttcattga atttcagcat gatttccacc tcttataatt
     2701 tacttttcaa gactgtatca aagtcttcaa aaatcttttt gttcaagtca atacctttag
     2761 ctactgcggt tttataagcg aatagttgaa acggaacgac aagcgctaat acagacagat
     2821 attcatcaca ttctattgct aatccaagtg ttttctcgtt tcttgctttt tttgtggtaa
     2881 tggtgagagt ctctccaacg taccgactca tatatctttg caacttttgc gaacgttcct
     2941 tatttacact ttccccttca ataaagaata gcaaatgcga agcatctgcc tccaggtatg
     3001 ggccgtgcat ataagcttct aactcaaatc cttgtgacgg aacgcggatt gtttcagtca
     3061 atttcgtctc tgcttcttta gctgttcccc agtttggacc atatccaatg gtgacgaaac
     3121 gctgtgctaa acgaaataaa ctttgatatc catcaaaaaa ttcttccgtt ttttgaataa
     3181 tcgctggtaa atggttgatt attttgcgca actgttgcat gtagtcttgt tctatttttt
     3241 tgctgatttt atttttactt attccaattc ctatccctaa gagtagtaac tgtaaaacag
     3301 tcgcagaata gccttttgta acgaagccta ctgattctac cccggcacct aaatcaataa
     3361 tttgtgcagc ttctaaagca agtggacttt gcacgtcatt tgtgatagca acaacaggta
     3421 acccttgttt ttgtatcatt tttactgctt caacagtaga agcacttttc ccgctttgtg
     3481 acacagcaat cacagtatcg attgaaggag aaagttttcc ataattgaaa aaataaaaag
     3541 gttcttgaat atcgacagta agatcaccat aagattccaa tgctggcttc gctgctaaac
     3601 aagcgttata agatgaaccc gttgctaaga tcagtaaatt cactgttttt ttcatatgtt
     3661 ctcttgtcga aaaatcattc tttttcaaga tcgtacttag tacttccttt tcttcataga
     3721 tataatcttg catcgtaaac attttctctg ctcctttttt aaaaaatccc tatccacgaa
     3781 ccaaaaattc caaagaaagc tagtccgatc aagatcgtta atggtttcac ttctttcttc
     3841 aatagataaa acactatgcc aaatgctcca agacataaga tatttggaac gatatcatca
     3901 aaaatacttt gaacagtcac agcattttct cctgaaccga tttttaaagg aatattgatg
     3961 gtgatcatcg tggcagtcat tgctccaacg accatcagtc caataataga cgcgcctaat
     4021 gtcaaacttt ccatcatacc gtttgcctgg atctttttca aaaaacccgt tcctaagcga
     4081 tatccccaac ctgttgctag atagcgaaac agaatgtgcg gaatattgaa aatcaaaatg
     4141 aacaagatag ggcctaaaat atttccttgc atggctaaag aggtacccac acctgtggca
     4201 atcaatcgca aagttcccca aaagaaagaa tctcctaacc ctgcaagagg tcccatcaaa
     4261 gatgttttga tactatcgat cgcagtcaca tcaaattcag gatcatcctt attttcttcc
     4321 tccattgcag ccgtgattcc taaaatcagt gtaacgatat gtggggtcgt gttaaaaaac
     4381 tctagatgcc gtttcaaagc tgctgccatt tcttctttcg actgatataa ctttttcaaa
     4441 atcggaatca ttgcataaac ataagccata ttcatctgac gttcataatt ccaagaaaac
     4501 tccatttgaa aagaacgcca aaatacgtga tttagctctt ttttcgttat tttgctgttt
     4561 ttgatctcag aattcttcat cttcttctac ctcccctgca gttgtttgaa gtggtagttg
     4621 acggacattc ataatattga ctaccacaac tgctaaaatc gcaccaaaaa tagctactcc
     4681 ggtcacagga atctctaagt aagaagctat agcaaaacct aagaaaaaat atggtgcaac
     4741 ttgtttgttg atcagcaaac gagcaagcat cgcaaaacca agagctggaa tcaaccctgt
     4801 agctactgac aacccctttt gaacgaagtc aggaatcata tttaatagat ttccgatcgt
     4861 attgcttccc accatataag aaaccattac cacaacacca agcatcaagg aaagaccgat
     4921 accggcaagc aagtgcatat gctcgactcc tttataattt ccttcttctg cataattgtc
     4981 tgctttatgg ttcataattg gaatcaaaag ccctaagtaa atatttttca atatcaatgt
     5041 caacgtagca atcggcaaac ctagtaaaag cgcggtttct gtaccggctc cagctgtaat
     5101 agcaaaagcc gtccctaaaa tcccaccggt cacaacatca ggtggaatcg aagcacctac
     5161 tgagaacgat ccgataaatg ctaactctaa agttgcgccc ataatgatcc cagtttttac
     5221 atcacctaac acgattcctg taaacagccc cgtcacaatc ggacgagaaa gcaacgatgt
     5281 ccctagtgca tattctgatt gtgcgacaaa tgcaacaatt cctaataaga ctgcttgtac
     5341 aatcattgag gttcttcctt tcttacaaaa cttctcttac atccactttt ttatctgctg
     5401 gtacttgacg gatttctact tctatttgtt tgtcaattaa tcgtttcaag cgttcctcct
     5461 cttctggtaa aatatttacc gcttttccga tgctgcgagt accaggtttt gccttgatcc
     5521 ctcctaagtt gatggttttg atttctggat acgcttctgc cagcttatat gcatcctcga
     5581 tcgattccac tacgatgaat aacttatatt tatctgtcac cccgcttttc aacgcggcaa
     5641 tcgagtcatc aattgatttg atcactagtt tgactccttg tggcttggct aatttgatcg
     5701 ttgtctttcg tatctcattc gtaggtacat catcattagc aatcaaaata cagtctgcgc
     5761 ctattgtttg tgtccatgaa aaagctactt gtccgtgtaa taaccgatgg tcgactcttg
     5821 tcaatacaat catttttctt cctcctaaaa ttcgtcttct tctattttct gattgagccg
     5881 ctcgttacaa aactggatca ttttttttga atcagttagc acctgctgga tcaaaacagt
     5941 tgtctctact tcagcgtcca accgagatac tagctcaatc aatagtggca aattcaatcc
     6001 ggcaacgaga tagacatgat cccgattgat atattgtaag aattcgttgt tgacacttcc
     6061 gccaaaaaga tccgtcagaa caatgatttc tttatctttg tacctcataa aaagctgcct
     6121 tactgtttcc gctaaatcaa aattctcatc agtatagcaa tcgaatacag taatagaatg
     6181 atttttccca tagatcagct ctaatgaatc taatatccct gaagcaaatt ttccatggga
     6241 agctaagata atttctctat ccactttttc atctcctctt ttcacttaca catactaaaa
     6301 aagcaagttg catgccaaaa ttagtggcaa caacttgctt atgttcaaaa ttcttcttct
     6361 atcgagagaa actctgtatc ttcactcaaa atatcataga tataggcaat ctcagaattg
     6421 gggatcatga cactataatg ctcttcaatg acactaaacg cagatttaat agcagataac
     6481 ccttcttttt gacactgctt ccatttctct aatccttgat aggtttcgat tggcatttgt
     6541 cggatcaatc gttcaatgag acagctcaca tggacataca acgctaattt tttgggattg
     6601 ctgatgcttc gattcaactg cacctctaat tctctcataa aatgatcgat ttctcccatc
     6661 accttctctg tatctagaat agtcacggaa tccagtactt tttccaatga aaaattacga
     6721 atgatgcgct gattgaatat ttccttctct gaagcattca tagtagtgtc caaccattca
     6781 gctagtacag acatcttttc ccccgagatg aggtcttcta atgaaagata gtcgatattg
     6841 gtgatatcag gatctgtggt gccaatcatt cctacgactt cgtatatgga aaataatggg
     6901 tcactgtttt tcttatcttt taattgctga tattcatatg ggatgatttt caactgacaa
     6961 gccgtaggta agcttttttc tagcagttca cttaagtgga ccgccgtacc gattccagtg
     7021 aagcaagtag taatgagtgc tttgggtttg ttttcctctg gatagagcag tcgccattct
     7081 ggctggatct gtcgaacagt ctcttctgct atctcttcta atgatacatt tttttgcagg
     7141 cattccccta ctgtgattgc taaaggtgtc gtcacattat tcaaaatcaa aagcggtacg
     7201 gataactgtt ttttaaaaaa ctgatggatt tctttcagag atcccatgtc tactaatatg
     7261 atcaatccat ttgaaacatc gctgttttcg caataatcaa ggatttctga agcaatctgc
     7321 tgtggtgtga catctagtgg catatcaaat gattcaaata gatcctcttg taataaacga
     7381 tttactacat ttgccatact gctagcagtt gcataaccgt gagcagcaat catcgctttt
     7441 ggcagtcgtt tttcttttga ccatttcgct ttttttagat agatcgtcag taaaatccga
     7501 tccatacttg tgtcttcaat atctaatttg atcttactta attccagaat tcgttggaca
     7561 tagtgataac tagatggata agactgagaa acttgttgtt ctaattcttt gattagttgg
     7621 ataatatcat gttcttccgg taaccatttg atagtgcttc gctgatagag atagtaactc
     7681 aaggcataga cactgtttcc atcgaatttg atttgatagg cagtttccat ttgtcgaaag
     7741 gtctctcgga tagattgtgt ccaataaacc agcagctcgt gtttttcttg tctactggtc
     7801 tcaaacagca aaaaatcaaa taattgaatc acttcttgct ttaattttcc ttcgcaagaa
     7861 tatagcagac agttattttt cttgaaatca attaggattt gctcaaaact tttaacgatt
     7921 cgctgctgtt cgggctgttg cttctccatt aattgctcta aggtctggtc ttgcgtaata
     7981 tagattcctt gctgtgctgt atttttagta ataggctgcg caagtagttc tttttgcaaa
     8041 tgatggatcg tgacatggat ttcaggctgt tcacgctgtt cagcatatgc ttttgctgct
     8101 gaaactttga cgatattttt cagctctcct atgtttccgg ataatttctg acttgttaaa
     8161 agtgacaaag ctggtccact gactgacaaa gggctcccca tttttctttg ttcttcgaga
     8221 aaaaaagagt aaatcagttc tagccgttca ttacgactac gctgattaag tgcaggtagt
     8281 tcgattcgga taggtattct tcgaatgaaa gttgttaaga aatgactagt aatctcttca
     8341 gtcgtagcaa aaaagagacg tgttttgatc cggcgtgaca aattaggttc tcccatacga
     8401 taaatcacac cttgatcaag ataggtgaac agtttttcct gaccttctgg tgttaagcga
     8461 tgtacctcgt ctaaaaacaa gatcccacca tctgctgctt caaaagcccc ttttttattt
     8521 tcttctgcac cagtgtacgc tcccttaaca tgtccaaaca aattgctagt caaaagttca
     8581 ggattatctg catattgtgc acaattaaaa gtgatgaatg gtgcagaatc ttctagcaga
     8641 tcatgaagca gacaatactg atagaccaaa ttaactaaat aacttttacc tgtgccactt
     8701 tcgcctgtaa tcaaaagtgg taatccgcca tctggatagt aaagagctgt tttgatttgt
     8761 tcgatacttt ctttcaaact ggcgtcgtat ccgatcataa aagagaataa gtcttttggt
     8821 tgcgcgaaaa aaggctgttc ctctttgaga tcatcgatag aaaaataaaa attcgtgcgt
     8881 aacttaaaaa attgtttttc aaaagcttct ttgtggaaat aaatcactgg tcgagaattg
     8941 attttgacta gtagttgctc atcatttaac tggttcaaat aatgactgac tgtgtttctt
     9001 tttaccccaa aatgatcagc taacctttgt gctgtaaaga cctcgttcat atgttctaaa
     9061 tcgaaaaagg ctgtctggtt ctctaagtaa cataaaattt ccttcttcaa caacttattc
     9121 acccctcctc catcatagaa aacggattca ttttgtcaat aaaatcttta ataaagaatt
     9181 gaaaaatata tcttattata gacagcagcc tacttatccc ttataatttt tattctaaaa
     9241 aacgtacagt tccttccagt ttttatactg gaatttcaac atatgaaaga ataaggaggc
     9301 tatgactatt caatgaaatc ggataaatat aaaaacagga aacatacttc ctggaaaact
     9361 tttcgagaaa tcatcggatc ttatctcgct gtagttgtat ttttgccttt gctttatatg
     9421 gctatgacag aacaaactcg ttttattggc gtagatacgc aatatatagc cgaaacagga
     9481 tttttattca tctttatctt gatttttgcg ttctataaat ccttccatac aaaataagat
     9541 tcacagattt caactttcct gtctgttttt agctattaca aatccttata tgtggtataa
     9601 caacaattta ttggcataat gaggaaaaac gagttatgat gctttcagag agtaaagagg
     9661 tactgttgtc accatccttt ttattgatta cgtacaccac tcttagtttt ttcccattga
     9721 ttacgaaaac aaggtagaaa cattgatgtt cctaccttgt tttctttttt tattttcaga
     9781 tcaaaatctc aagtagcttt tgacttgatg tcgggaggtt gtcttcgttc catcaaacct
     9841 gatagaatct gcgccaaacc atagttttct ccactattat ttattcctct aagatagtac
     9901 ccacaataaa cgaataaata tttttctcat tttcatactc cttctttgaa aatcaattag
     9961 atttctctca acccactttt ttacaaaaat tacgtagcaa aaaagattgc aagaacaaat
    10021 taaaaaagaa gtaaatagag tttcatctat aaacatttac ttgatatatt ttttatgcag
    10081 aattcattta tagaaaaatt aagtaaaata gacgttccta tgaatgaaga gactggcatg
    10141 ttttctcatt ttcttttttt taaaaaaaat agtctattca atttctaaag cttcttcata
    10201 ataaaacttt catttatgaa aaggcttgtt ctcttttcat tctaaatttc ctaagctaca
    10261 atgtggtagt acttgttttt tcaattgtgg acaaataaat aataggagga atcatatgat
    10321 tcagttatta cgttatgcaa aagactatcg taagcaaatc attttgggcc cgttcttcaa
    10381 atttcttgaa gctgtcttcg aacttgtcct gcctttaatg atggcttcct tgatcgataa
    10441 tggactaaaa atgaatgacc gaggaaaaat cattgaaatg ggcttatgga tggtcgctat
    10501 gtctgtgatt ggtctgattt gtgctatcat ttgccaatac tatgcttcag tagcttcaca
    10561 aggatttggt acagagttac gtaatcagct aatcaaaaaa atcaatactt tctctcacaa
    10621 cgagttgaat cattttggaa cagatacctt gatcacccga atgacaaacg acatcaatca
    10681 gcttcagcta gctttagcaa tggtcattcg tttattgatc agagcaccgt ttttaagtat
    10741 cgggtcagtg gtcatggctt ttgtgatcga ctgggaagta ggtctgtttt tcttagcatt
    10801 attgcctatt ttctcaatca ttttattttt tatcatcaaa aaaaccgttc ctctctacca
    10861 aaaagtgcaa gaaaaactag atcagctcaa tgaacacgtt agtcagaatc ttagcggtgt
    10921 tcgtgtcatc cgcgcatttg cgcagacaaa aaaagaaaca gagaagttcg ataaaagcac
    10981 aaatgatcta gcgaacaatt atttacgtgt ttccaatatc tctgccctat tatcacctgc
    11041 tacaaccttg atcatgaatg tcgggatcat ctgtttgctg acagttggcg ggatcaaagt
    11101 aaatattggt agcttgcaac aaggacaagt tcttgctttg atcaattata tgaatcaaat
    11161 gcttttggca ctgatcgttg tttcaaacct cgtcgtgatc ttcacacgtg ccgaagcttc
    11221 aggaaatcgg gtgaaagagg tgctagacac agaaaattcg attcttgaag ctgaggtcag
    11281 tactactccc gatttttctt ctgaagcaat tatcgctttt gaccatgttg acttccgtta
    11341 tactcctgat tctggtcttt ctctccaaaa tattacattt cagctgaagc gaaatacagt
    11401 ccttggaatc attggtccaa ccggaagcgg gaaaacgact ttgacacaat tgattccgcg
    11461 tttctatgat gtcagttccg gtacagttat aatggacgga atggatgttc gttcttggca
    11521 gctggatcat ctccgtcagc aaattgctct tgttccccaa acttctgtac tatttaccgg
    11581 gactattcgt gaaaaccttc aatggggtaa gaaagacgcc acagaaaaag aatgttggga
    11641 agctctgaaa atcgctcagg cagaagaatt tgtccgtcag cttccaaatg gtttagatac
    11701 aaaagtcatg gaaaatggga aaaatttctc tggcggacaa aaacagcgac tgacgattgc
    11761 aagagctttg attgccaagc cagcattact gattttagat gactctttaa gtgcgttgga
    11821 ttatcaaaca gacttggatt tgcgtaaggc gctacagact tatcttggca gtacagtgat
    11881 tattatctcc caacgtgtcc gctctattca agaagcacat catatcttag tgatggatca
    11941 aggtaagatt gctgctcagg gtacccatga agaattactt gctcaatcag cagaatatca
    12001 ggaaatcgtc gcatcacagg aggaagaata gttatgaaag taaaaaaatt atccttgcag
    12061 acgctccgcc gattcattcc ttaccttact gcctatgcaa aagacatttg gctagctgtg
    12121 atactaggaa tcatcaatgg gattgcaaca gttgtgatga cgttacaaat tggtcaaagt
    12181 atcgatcaaa tgattggaga aggacaggtc cgcttcaatc gactgttcca tttacttatt
    12241 ctattcggtg gtatcgtttt aatcaacgta gtcagtcaat ggctcatcca agtgttaagc
    12301 aatcggttag cttacttatc cgttgcaaaa ttaagaaaag atgcatttgc tcatctgaat
    12361 cgattgccac ttcgttatta tgatcaaaac tcccacggaa atatcgtcag tcgatttaca
    12421 aatgatatgg acaatatttc cattgcaatt tctgcggcct tcaatcaatt attttctgga
    12481 gtggcaatca tcattctttc gtttattttt atgatgcgtc taagtccact tctgacattg
    12541 gtcgttcttt gttctacacc aattattttc cttgtgagtt ggatcgttgc tcgtgcttca
    12601 caaacaaatt tcgatagaca gcaaaggatt attggggata tctcgggctt tgtgaccgaa
    12661 cgaatcggta accagaaaat cgtgaaagct tttcagcaag aagaagctag tcaacagcaa
    12721 tttgaactgc taaatgctga tctatatgaa aaaggacaac gggcacaatt ttcttcttcc
    12781 ttgacaaacc cgtcttctcg ttttgtagat caccttgctt atttatcgat tggtttagtt
    12841 ggcggcctat tggcattgaa gagtggttcg tcaatcactg ttggtgtgat ttccagcttt
    12901 acgatctact caagtcagtt ttctaaacca tttatcgaac tttctggaat cacgacacaa
    12961 ctgcaaaccg ctttttctgg tctagaacga gcatttgatt tgatcgatca gccagaagaa
    13021 aagcctgatt tgccagatgc ctatgccttg gaacccaaaa atatcaaagg aaaagtctct
    13081 tttgatcatg tttctttttc ttatgacccc aagcagcgtt tgatcgaaga tttcaatttt
    13141 actgcttatc caggtcaaac cattgctata gtaggaaaaa caggggccgg aaaatccaca
    13201 cttgttaatt tgctgatgcg tttttatgac acaacaaaag gaacaattac agtcgatgac
    13261 catgacatcc gctctatcca acgtgataca ttacgtaaaa gttttggaat ggtgttacaa
    13321 gatacttggc tatttgacag tacattgcga gaaaatcttc gttttgggaa tccggaagca
    13381 agtgacgcag aaatcaatga tgccctaaaa gcctcttata tgtatgaatt tgtagaacgt
    13441 ttgccggaaa aattagatac taagattggc ggtcaagggt taaagatatc agatggacaa
    13501 cgccaactgt tgacgattgc tcgaacgatg atcagtaatc cccctatgtt gatcttagat
    13561 gaagctacca gctcagtcga tacattgaca gagaaaaaaa tccaacaagc cttcttagca
    13621 atgatggaag gaaaaaccag ttttgtgatt gcccatcgcc tgtccacaat caaaaatgcg
    13681 gaccagatat tagtgatgga tcagggagct attgtcgaaa tgggcaccca tgatgagctg
    13741 cttcaaaaag atggttatta ccgacggctc tatcaagcac aattcacagc acaataaaaa
    13801 caagcagtct tctgggatgg gaagactgct tgttttt
//
LOCUS       DAFEPQ010000069        13537 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.071, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000069
VERSION     DAFEPQ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.071"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(60..1007)
                     /locus_tag="LP543_002184"
     CDS             complement(60..1007)
                     /locus_tag="LP543_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBL2322336.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1197..1466
                     /gene="rpsN"
                     /locus_tag="LP543_002185"
     CDS             1197..1466
                     /gene="rpsN"
                     /locus_tag="LP543_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBL2322337.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1642..2004)
                     /locus_tag="LP543_002186"
     CDS             complement(1642..2004)
                     /locus_tag="LP543_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL2322338.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2015..3136)
                     /locus_tag="LP543_002187"
     CDS             complement(2015..3136)
                     /locus_tag="LP543_002187"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBL2322339.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYIHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3152..3502)
                     /locus_tag="LP543_002188"
     CDS             complement(3152..3502)
                     /locus_tag="LP543_002188"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBL2322340.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGKVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3632..5143)
                     /locus_tag="LP543_002189"
     CDS             complement(3632..5143)
                     /locus_tag="LP543_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL2322341.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQSETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5465..6817)
                     /locus_tag="LP543_002190"
     CDS             complement(5465..6817)
                     /locus_tag="LP543_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBL2322342.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKDFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVPDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAVKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(6970..8046)
                     /locus_tag="LP543_002191"
     CDS             complement(6970..8046)
                     /locus_tag="LP543_002191"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBL2322343.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            8242..9987
                     /locus_tag="LP543_002192"
     CDS             8242..9987
                     /locus_tag="LP543_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBL2322344.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDSVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLKKEEQTQASRKTEIVHEKEFGSFAFRASTSTEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10236..10703)
                     /locus_tag="LP543_002193"
     CDS             complement(10236..10703)
                     /locus_tag="LP543_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBL2322345.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFCQALKKAIDQGLPSTEDSR"
     gene            complement(10687..12060)
                     /locus_tag="LP543_002194"
     CDS             complement(10687..12060)
                     /locus_tag="LP543_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322346.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGIMYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDAAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12381..12869)
                     /locus_tag="LP543_002195"
     CDS             complement(12381..12869)
                     /locus_tag="LP543_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2322347.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(12891..13493)
                     /locus_tag="LP543_002196"
     CDS             complement(12891..13493)
                     /locus_tag="LP543_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL2322348.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3863 a         2938 c         2458 g         4278 t
ORIGIN      
        1 ttttgtcttt gcccctacaa aaatgtttca gcttttttcg ttttttattc ctgtatttct
       61 tatttttcga atgacaggat gattttccca gcaggaggat gtccttcaac tagctgatgt
      121 gcttggatga catgcttaag ggaagcagac agattcattg aaataaaaac atgaaaggct
      181 cctttttggt aggctttgat tatgccagca aaggcttctg catctagata ttcctttctt
      241 ggaacaaacg attcataaaa gccttccttt ttttgccgca aatctagatc tgggaggtca
      301 tttaatgcaa catacctgcc gcctggtttc atgatctgga tgccggtttc tcctttgata
      361 cttcctttcg tcgcatcgat tacaaggtct gcctgatcag caaattgcag acctggattt
      421 gttttatcat aagcagcaaa tgctgaagct cctaattttt ttaccttttc ctcattttta
      481 ctggatgcac tggttaagat tcggattccc ttttcatgga gaagctggat cagacttgac
      541 ccgaccgcac ccgatgctcc tagtatcatt acagtatctg tcggctggat ttccgtcaaa
      601 tggttgatta gattataagc cgtaattcct ggcgtgacca ttccggctgc ttcttcccaa
      661 ctcattttat ctggaatttt tgctactttt gctgatggca acacgacttc ttctccgtaa
      721 gtcccgccga ctgcatggac agctactcga tcacctacat gaacagttgt gacatctgaa
      781 ccaatctctg tcacaatacc agcgccatca ttacctggta cataagggaa tttcaatgtt
      841 cgtatctctt tcatttctcc taaacgcaag gcaacatcat aaggattcac actgaatgct
      901 tttatttcta cacgaacatg tccagaaccg acttctctgg gatgagcatc gatttcttca
      961 aaaacatctt gtgcttctcc gtaaccgtta attgcaaatc ttcgcatggt caccatctcc
     1021 ctttttcttt aagtttacgt ttatgccttt attttgacaa aaaaaatgtc ttttcgtttt
     1081 ggcgcatgat tcaaaacaaa atagttgtct tttttcgcgt aacgtgttaa attatttatc
     1141 taaaagcgta aagattacga tttacgctaa taaaaaataa ttggaggaat ttgttgatgg
     1201 ctaaaaaatc aaagattgcc aaagcaaaaa aacagcaaga aacgattgaa cgctatgctg
     1261 ctttacgcca ggaattaaaa gcgcaaaaag attacgaagg cttgcgcaaa cttcctttag
     1321 atgctcgtcc agaacgattg aaaaatcgtg atttgatcga tggacgccct cgcggataca
     1381 tgcgtaaatt cgggatgtcc cgtataaaat tccgtgaatt agcacataaa ggtttgattc
     1441 caggagtaaa aaaagccagc tggtaatcca agtctggctg taatgtaaag gaggagggcg
     1501 catggaccac tccttggatc atcaaaaaag atacgttcgc ttcttctagc agaaatgctg
     1561 gtactagaag cagacgtatc tttttttacg cacagctggt ccttttttat atgtaaaggc
     1621 tgcatggtta cttcgctata tttatttagt aacaggagtc agtgcttcga tacccacact
     1681 aattttcaat gctcggtcaa cttctgccat aatgggtcga tccaaatggc agaccttttc
     1741 tttcaatctt gatttatcga tcgttctgat ctgctccaac aggataacgg agtctctttc
     1801 tattcctgtt ccagtagctt tgatcccaat atgagtcgga agcttaggtt tagccatttt
     1861 tgccgttatg gccgcaacaa tgatcgtcgg gctaaaatgg ttacctagat tattttgaat
     1921 aaccaaaact gggcgtgttc cgccttgctc cgatcccacg actggagaaa gatcagcaaa
     1981 ataaatatct cctcttttta ccataggatc ctccttattc ttgatattct cttggtatcc
     2041 gctgtcctaa tccgcaagcc acttcatagt ggatcgtacc taattgatct gctatatcct
     2101 gcatagtgat ttctttgtct gcatttttgc cgataagcgt cacttttgtt ccgacaggaa
     2161 attcttgagg caatcggatc atcagttgat ccatacatac acgcccaatc gtttcacagt
     2221 agttcccttc taccaataga gaaaaaccct gcatctttcg tggccagcca tctgcataac
     2281 cgatcggtat cgttccgatc cactctgctt caggagtgat atatgtttct ccatagccga
     2341 ttccttcacc tgctgggagt tttttgactt gaattaattc agaaaccagc tcaagtgctg
     2401 gctgcagtgg ataaacttcc ggtagtgcgt gcccagaggg attcagtccg tacattgcta
     2461 ctccataacg aatcatattg ccgattgttt catcatgcca taaagccgtt gcactattgc
     2521 tgacatgtat atatcgagga agttcgggaa gtttttttag cacttctata aagcgttctt
     2581 tttgcagatt ccaatatgta tcatctgcct gatctgctgt agaaaaatgg gtaaataaac
     2641 cttcccattc taatacacga ctttctttga tgaatgcagc tgcctctttt acttcttcag
     2701 gtgtacaaaa tccgatacga cccatcccag tgtctgcttt tagatgaatc gaaagaggcg
     2761 tctctagctt acttttctct aaaacagcag ctgctgcctg caaccattcc cgtgtacctg
     2821 cagttactga taaatcatga gcaacagcta cagtcagtgc gtcgtaagga accatattca
     2881 agataaggat gggctgagta aatccagctt ctctcaactc gactccttca tctaaatttg
     2941 aaacacaaaa gcccgaagct ccacctgcaa ctgctgcctc agctgtttca atagctccat
     3001 gaccgtatcc gtttgctttt actactgcaa acaattcttt tccttgcgga agtcttgcaa
     3061 cttcatttgc tacattttca gttatagctt ttttatgtat cactgctttc gttgggcggt
     3121 gccaagcgac aaccatcgtt tttccttctt tctagtcttc caaaataatt tgtgcaaaag
     3181 ctgtttcatt tgtatgagta atactgaccc atactttgcc ttcatgaggt gaacgcgtca
     3241 caacaggctg accgttttca ttttttaaaa tctcgacatc ttgaaaggta acttttccga
     3301 tcccagttcc ccaagcttta gagaaagcct ctttgcaagc gtaacgcccg cctaaaaatt
     3361 ctacttggcg atgaaaaggc aaggactgga atagtttcat ttcatcagag gtcaagattc
     3421 gtgcaatgaa ttttggtttt tcttcgatca gccgagtgat cctaggaagt tcgactgcat
     3481 cgatcccaat tccttttatc atatgctttt ctcttttcaa taaatttaga acactctcat
     3541 tctaccaaat tataaccatt ttgccacaaa taagtccaca aataacaaaa atgaaagttt
     3601 ctcttaaaaa agagaaactt tcattttatg tttaatcgtt atttgaacgg attacaaatc
     3661 cgcgtttttt atcgttgtga cgtttaccgc catctttttg gctgttatta tttttgctat
     3721 aaccgccttt tgatttgttt ccacgataat taccgccgtc acgacgatta cggttgtttc
     3781 cgccaccacg tttgccattt ttattgtatc cttttttctg cattggcaat gggcgttctg
     3841 gtgtgatctt cactggcact gcatcagctg gatcttttgc tgtcgttttt agcaaaagtg
     3901 caactaaatc ttgtgcagaa tattcttcta ataatttatc tgctgtttgc aggtacttat
     3961 ctagaccatt ttctgcaagt tttgtttcga tttgttcaac tgcagcacct aattgacctt
     4021 tgaatgcttc tttttcggtt ggtggacgta aagtcgtcat ccgttttttc gtcaagtttt
     4081 caatcacatg caagtagtcc atttcatttg gtgtaacgaa agtcacagac ataccgcctt
     4141 ttccggcacg gcctgtacgt ccgatacggt gaacgtagct ttctggatct tgagggatat
     4201 catagttgta cacatgagtg acacctgaaa tatctaatcc acgtgcagca acgtctgtcg
     4261 ctacaaggat atctaaatga ccgctcttaa atgaacgcaa tacgctcatt cgtttttgtt
     4321 gagacaaatc gccatggatt ccttctgcac ggtaaccgcg tgcttctaat ccgcgtgcca
     4381 attcgtctac acggcgtttt gtacgtccga agacgattgt tagttcaggt gtttgaacat
     4441 caaacaagcg agtcatgata tcgaattttt cgtattcttt tgcgcggaca tagtattgat
     4501 cgatcaagtc agctgtcatt tctttcgctt tgatcttcac atgatgtgga tttttcatga
     4561 atttaacgcc aatattcttg atagctggcg gcatcgttgc tgagaaaagt aatgtttgtc
     4621 tttgatcagg aacttgtgaa atgatttttt cgatatcttc caagaagccc atattcaaca
     4681 tttcatctgc ttcatccaaa actaatgttt gaacggttcc aagtttcaat gtgtggcggt
     4741 tgatgtggtc aagcatacgt cctggtgtac ccacaacgat atgtggacga tctttcaagc
     4801 cacggatctg acggccaata tctgctccgc cgtaaacggc ttgtacacgt acttttttgt
     4861 cgcgccctaa acgatataat tcttcttgtg tttggatagc taactcacgt gttggtgcga
     4921 taactaaacc ttgtaattcg tgacgatctg gatcgatttt ttctaacatt ggtaatccaa
     4981 atgcagctgt tttccctgta cctgtctgtg cttgtccaat aacgtctttt ccagctaatg
     5041 ctagtggaat cgtttctgat tggataggtg ttgcttcttc aaaaccagcg cgttcgactg
     5101 atttcaacaa ttctggtgat aattctagtt ctttaaattt caaatgaatc ctcctatatg
     5161 gttgttaact gaggatgtag cttttttgct accccgtcga tttgttcggt caaagtgtat
     5221 tgtttctttt tgacagtggc cacgcacgtt tttatccatt cttagcccct gtggcaccac
     5281 cgaaaaatca ctttgcgaag tttaggcatt agaaaagcca aacgggtcgc gttcagcttt
     5341 atcattatta tgacatctgg cttcaacaag ttgtttctat ggcaaaacca gtccaatttc
     5401 tcttcatctt tcggcaatag gttattctaa catagttttt cgttttctgc aagtttacag
     5461 atttttattt catttgctga agctgttcta ccacttcaaa taagcccatg ctgttgctgc
     5521 cttttagcat aatactatct gttgcttcaa gattttgttt tacagcatca atcaatttat
     5581 tcttttcttt ttcaaaatag aacacaggta gttgggggta tttttggttc aattcttctt
     5641 ttagtgcatg catttgttcc ccataaagga aaatcatggc gaatttctca ggatcgatat
     5701 gttcactcat ttgcgcatgc atatttaaag actctttccc taattccagc atatctgcta
     5761 acacagcgat tctgcgtcct tttgttggta gatttttaaa agtatctaat accagtccca
     5821 tggctgttgg atttgcatta tagacatcgc tcaaaatagc tgcaccattt cctgccgtta
     5881 gccattcggt tctgttttga gtgatagaaa catgggcgag cccttggcgt atctcaggta
     5941 tggacagtcc aaagaagcgc ccgatcgtat acgcaatcaa agcattcgtt acattataac
     6001 ttccaggcag cgggatcgta aatgtttctc cttctactac aaaatcggtt tgtgcttttc
     6061 cttttgctgt cacttccgca tgaacatcgc catttccaat accgaatgtt gtgactgttt
     6121 gtactaatgt tgccgttaaa ggtgtcagca acggttcatc tgcgggcaca acgagtaatc
     6181 cgtctggtac aagtccgtca gtgatttcca ttttggcttt agcgattcct tctcgcgacc
     6241 caagattttc tatgtgtgct tcaccgatca ttgtgattgc tgcagctttt ggttgagcca
     6301 taagtgaaag ttccgtgatt tcattggcgt gatccatgcc catttccaag acgatctttt
     6361 ctgtatcttg gggcatgtgc aaaatcgtgt aaggaagacc aatattattg ttgtaatttc
     6421 cttgcgtctt atatgttttg tatttttcag acaagacaga cgcggttaga tctttcgtgg
     6481 tggttttacc attgcttcct gtaattgcaa cgacttctgc acctactttt tccaagtaat
     6541 aggttgccaa ttgctgcatg gctttcaaag gatctgctac aggaataact ggaaaatctt
     6601 ttggagcttg ttcaacaggc aagctccaaa atgaagcaga tgctccgttt gtgatcgctt
     6661 gctggataaa ttcatgtcca tctcgcgtac cagcaagagg aacgaataaa cttccttcgg
     6721 ttatcttccg gctgtcaaat tcgacattgg taatctttgt cagtgcagag cattcgctgc
     6781 ccacggcttg tgcaatttct tgtattgtta gattcattga gatctctcct aaaaaaggtt
     6841 gtgaaaaaag cgtttggcct aaacggcggg ttcgcatgac tctcacttcc aagtaaattt
     6901 ctctattcgt atttagaagc tagaatgaac gcggttacgc aatcactcca gcttcttctt
     6961 tatttctctt tattcatttt tttcaaaaaa agattgacgc tgatggtatc gattcattcc
     7021 taattgaatc agttcttcaa tcaaatcacc gtatttcaag cccatatttt cccataagag
     7081 tgggtacata ctgaactccg taaatcctgg catagagttt aattcattca ggaataattc
     7141 gtttttattt gtcaaaaaga aatcgcaccg gctcaatccg cttccaccta acatcgtgta
     7201 agctaacttc gcgtactctt gcgctttttg ataaacttct tccggcactt cggctggaat
     7261 ctgcatttcg attttattat tgatatattt ggcttcataa tcatagaatg ctacgtcttt
     7321 tacgacttcg ccaggcaaag tcgtccgaac atcttcattt cctaatacag caacttcgat
     7381 ttcgcgcgct tcaattcctt gttcaacgat tgctcgagaa tcatactgat aggctgttgc
     7441 taaagcattt tgcagctctt ctcggttttc tgcctttgta atgccgacac tagaacccat
     7501 attcgcaggt ttgacaaaca tcggataaag caaagaacct tcacattgat caaatacttt
     7561 tttaggattt tctttccatt gattcttaag tactggtaca taaggaactt gcggcacacc
     7621 agcagcttgt aaaatatact tggtcatgat tttatccatg gcacatgcac tggtcaatac
     7681 gcctgcgccg acataaggca tattcaatgt ctctaagaag ccttggatcg ttccatcttc
     7741 cccgtttggc ccgtgtaaaa ctggaaaaac gatggctcct tcttctttga tttctcccgg
     7801 attgatcact tttcctgtaa agccttcctc tgtctgtcca cttgggtccc atgaaagatg
     7861 caagacatct ttgctagcag gtttttctgt tagtaatgga cctttgaccc attgtccttc
     7921 ttttgtaata aatacgagtt gaacttggta ataattataa taaatggcat ttaaaactga
     7981 aaatgcggaa agaatggaaa cttcatgctc tgcgctgcgt ccgccatata gtaaagtaat
     8041 cttcaaaaga ttgtcctcct gtattctaaa tcgttcagtg atttactctc atattttagc
     8101 acaaaacaaa aataaacgaa taacttattt ataaaaaaac gagttggttt ctaaaagaac
     8161 aaacgtcatt tattttttgt cagctagatt atttgtcccg ttttttataa aaagttatga
     8221 taaagaaaag gagggatgat gatggcgcaa acaaatccac gtagccgctt ggcagaaatc
     8281 attcgagtgt tcatcaatta caatgtagtg ccaaactttg tacagcaaaa aaatcccgaa
     8341 caggtaaaaa aagcctttga agaactaggc cccaccttca ttaagatagg acagatgctt
     8401 tctgttcgag aagatttgct ttcttctgcc ttcactcaaa cattcaaaac attacaagat
     8461 agtgtaccta gcgacacatt ttcaacggtc aaaaagacga tcgaaacaga actttctctt
     8521 tcattatcag atatattcga tgatttttcc aaatcgcctt ttgcctctgc ttctatgggg
     8581 caggctcatc gcgccaagct gaagaacggt gactcggtcg tcgttaagat ccaacaccct
     8641 aacatcgccg aagagatccg acttgatctt caactatttg aacgagcgat ccctttgatc
     8701 aaatacattc ctgaaactag cgtagttgat ctaaaagggg tactccaaga agtaaaacgt
     8761 tccttgatca atgaaatgga cttcttaaaa gaaagtcaaa atggcgaaca attctatcaa
     8821 aagaacaatg gctggaaaga gatccgcagc cccaagattt atgacgcttt ttgttcgaaa
     8881 aaagtgatcg tgatggaaga aatgtctggt aaaaacttga accatttaat gaacgccgaa
     8941 aacaaaactg aaacttttat cactggaatt caaaacaagc agctgaaaca agaagttgca
     9001 aagcttttag ttgagaactt tatgaaacag gtatttgatg atggtttttt ccatgctgat
     9061 cctcatccag ggaatctgct attccatgta ctaaaaaaag aagaacagac acaagctagc
     9121 cgaaaaactg aaatagtcca tgaaaaagag ttcggttcct ttgcttttcg agcctccact
     9181 tctacagaag accctgttgc gccatatacc atcaactaca tcgattttgg tatgatgggg
     9241 catctttctg caggacttag gcaaaaactc acacaagccg ttttagcgct gtatacgaaa
     9301 gatgcttatc gaatcgaaaa agcggtcctt cgactttgtc agcaagaagg ctcgtttgat
     9361 gaaagtcggt tccaccaaga actgacaagt tttcttgaac agtattatga ttctcctatt
     9421 gatgaaatca acttgcaaga agtattcaca cacgtcgtga cgatttgtca tcaaaataat
     9481 ctgcagttcg atcgtgatat cacgcttttg ctgaaagcat ttgggacatt agaaggggtg
     9541 atccgcgtcc ttgaccctga agtttccctc atggaagtcg cttcgccttt tgcccaacat
     9601 tattttctca ctcatctgga tgtagaagat acattgaaac aaagcggatt agacttattg
     9661 gaagggatga aggctgcgcc taagatccct caacagctcc accatctatt agaaatgtgg
     9721 acttcgggac aaggaaaagt caatttggaa ttgaaaaagc aagacaaatt attgagcagg
     9781 atcgaaagta tgatcaatcg gcttgttttc ggtatgattt tagctgcatt gatcgtaggg
     9841 tcctccctgc ttgtccaagc agcacctgta gaaaacgcag aggtcgtttc cttattaggg
     9901 atatttacgt atgctattgc cgcattcgtc attatttttt tggcaatcga tgcattgatc
     9961 caaatgtata agaagaggaa aaagtagaaa aaggttgtga cagaaagaat cagcttcaag
    10021 aaataagaag tgattttgca taaattgttt ctcaaatttt tgtgaaaatt cggcttattt
    10081 ttcgaagagg ctacttctga agcaccgaga ataaaaaatg tgaaaaagct gttctgcacc
    10141 cagtaataag aacaatcaaa aaggaacatg gagcacattt atcctgtttc cctgttccct
    10201 ttttgatgta ttcgagcata aataaaatac cttcgttacc tactatcttc tgtacttggt
    10261 aaaccttggt caattgcctt ttttaacgct tgacaaaaag acatatgatc tttctccgta
    10321 aagggttttg gaccctttgt ccttatgcca gcttttctta actttccact atgataacgt
    10381 tgttctagaa gcgtgccgat attcgcttcg tcaaattctt ttccagagtg atggaacact
    10441 cggacgccct tcggcaatac aagtgaagct aaaccataat cttgcgtgat caaaaagtca
    10501 ttcttcttaa ccataccgac gattttataa tccgcactat cagctccgcg gtctacgtag
    10561 acgatttgga catgattagc gtattctttg gtcgtataat ggtccacact ggtcacgata
    10621 accacgtcaa gaccatagga ttctcctaat tttatgacat cttcttttac tggagaacca
    10681 tctccatcaa tgaatagcct cattcttttt cgccttcttc cataaatgat aaacagttac
    10741 taatccgctc agtaaaagac caacattgat ccattttgcc aagataagca gttgtacact
    10801 ttctatcggc actgattcat ggttcagcgt gatcaataat agatggattg gctctcccat
    10861 ttcttttgct aaaacaaaca aaagcgtcat caataaaaac tggtggatca cactcccttt
    10921 attttgagta gtatacttga caacgatccg gcgatccatt ggaatagctg catcagtttg
    10981 ataatattcc ttatttagcc agctgcctgt tactacgtgc cagaaactaa gcagaaagac
    11041 acctatcgca aaaccatttg gaatcaagag gaccaataaa gaagaactca gtccaagcaa
    11101 ttgcatagcc aatggagacc atgttaaacg tttcaaaatc catggtccga ctatttttgc
    11161 tgcaatcaag cctgctgcat aaggcaaata cataaaaatc gaaagttttt ctaatccgta
    11221 cattatgcca acataaaaac tgctaaataa aaaaaggaaa ttccctagca ttccatttag
    11281 aaaagtaaac agatttagcc agcttggcaa cttccattcc ttcttctggt gtcctaaaac
    11341 aaacacgaac aagatgaaca gcagcaagaa accataaata gcaagatcaa aaagcctttc
    11401 atcgaataac aatcgggcat ttctcaaaag aaacaataag ccaaagaata tcaaaaataa
    11461 aacaagctct cgcgtagcta ccagatttct tttgatgtct ttgaaatcca gttcgtaacg
    11521 cggataatga ctaaccgtat ggtaggccat gacgtaaaaa agtgtataaa tagtaaagct
    11581 tgctgcaatc tgagtcggtg caggcaactc aatcgcttga tacaggagaa atagccagat
    11641 agctgcaaat aagtactttt tcccatccat attgataaaa ccttgcgtct tttcgtgaaa
    11701 attgaccgtg gtattagcag gtacaagcca tgctgcactc agccctagca aaacacttga
    11761 aaaataaaat aaaggaaaat aaaatacgcc taatattcca agcaatgaac cagcaccacc
    11821 aatcagcaga gcgatcatca gcaacgtgta actatctaga ccaaatcgta gactgttgac
    11881 taaaaatagt ccagtcatcc gaaaggcata aaacagcgta aatggcaaaa tcgtcagcca
    11941 attatgcgag tctgttagtc ccaaaaataa aagataagga ataaaaataa ccgtgttact
    12001 taagataaag ggcgttgttt gtgtaaaatt acgcccaaat cgtttgagtt tttcgaacat
    12061 gacaatggct ccttaaacag tcttataact aatctgctct tcgattttag cattaaaggg
    12121 gaaataaacc aattttacag aatatattct tgtaaaaaaa atacagaaag aagcataagc
    12181 ttctttctgc atttttacgg tacttgatta ctcaagcaga caagcccttc tccacaaaag
    12241 tcttgcccga cacccctgca tccggcaggt gataaagata gaaatcaatt atcctgtgcc
    12301 tccaaaatca attaggagct ctgtaaacca gagtccttct cgacccgtcg cataaattta
    12361 ctttaacaca gttcctatgt ttatgcgaga gcttttttca ttttataatc gaccatttga
    12421 aatccttgac cgatatctga aataagttct tctgtaatct caaatccttc atgcttataa
    12481 acctcgattg cttgatgatt cccctgattc actcgcaaaa agatctctct ttcctttgtt
    12541 tgcgcagcaa gttcttcata ccagcgaaaa actgaagaag tcaatccttg accacgtact
    12601 ttatttagca aatataattt acttaaataa atttttccat ttatctcttc ataagcagtg
    12661 taaccgacag tcttttcttc gctttttaat aaaaagtagg acacaccttc tgtcagttct
    12721 ttttgaatct gtttttttga ctgataagtt ttcagcatgt aatccacttg tttttgacca
    12781 atgatcggca caaaagcttc cggccatact tcttgtatga ctgccatcag ctcatccagt
    12841 tcattttctg ttacttttat tttttccata cacccacgtc cttttttaca ttataaatga
    12901 ccttgcgctt gatggaataa aaagagcatg atcatgctat acacacccac cgaccacggt
    12961 ttcaagaaaa tcacaatctg cataaattcc ctaaaagata atttcgtcaa accagctact
    13021 agacagatca agtcgtctgg cgcaaaggga agcagcaagg taacaactag tactttttta
    13081 atatctttcg tttttcttgt cagcaattca tcaaatttct gatacttttt tggcgataaa
    13141 ataagctgga caaaggctac gccatagtaa cggacaagca aaaatccgat tgcttcccca
    13201 atcactaggc caatacaact atataaaagc ccaaatccat tgccaaacat cagcatgccc
    13261 gctactgagg agatccctcc cggcaaaata ggaatgatca cttggatcac ttgcaacaga
    13321 acaaatatca tcactgcata cttgccaaat tgctggataa acgcttgcaa agacgtgctc
    13381 gatcgaaaaa tcccatgatg ataagcataa agaatcaaca caaagaaaaa aaacagacca
    13441 ataatgggca tgatctgaag acatttttgt aaaactgatc cttttttaga catctatatt
    13501 ccccacatct tttttcattt attaaaactg cctcctt
//
LOCUS       DAFEPQ010000070        13346 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.072, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000070
VERSION     DAFEPQ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.072"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(45..1364)
                     /locus_tag="LP543_002197"
     CDS             complement(45..1364)
                     /locus_tag="LP543_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL2322349.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="LP543_002198"
     CDS             complement(1361..2014)
                     /locus_tag="LP543_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2322350.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2822
                     /locus_tag="LP543_002199"
                     /pseudo
     CDS             2302..2822
                     /locus_tag="LP543_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2724..3440)
                     /locus_tag="LP543_002200"
     CDS             complement(2724..3440)
                     /locus_tag="LP543_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBL2322351.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3564..3749)
                     /locus_tag="LP543_002201"
     CDS             complement(3564..3749)
                     /locus_tag="LP543_002201"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322352.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3976..5249
                     /locus_tag="LP543_002202"
                     /pseudo
     CDS             3976..5249
                     /locus_tag="LP543_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5356..6387)
                     /locus_tag="LP543_002203"
     CDS             complement(5356..6387)
                     /locus_tag="LP543_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2322353.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6394..7224)
                     /locus_tag="LP543_002204"
     CDS             complement(6394..7224)
                     /locus_tag="LP543_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2322354.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7221..8036)
                     /locus_tag="LP543_002205"
     CDS             complement(7221..8036)
                     /locus_tag="LP543_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL2322355.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8033..8857)
                     /locus_tag="LP543_002206"
     CDS             complement(8033..8857)
                     /locus_tag="LP543_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBL2322356.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8847..9947)
                     /locus_tag="LP543_002207"
     CDS             complement(8847..9947)
                     /locus_tag="LP543_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL2322357.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10125..10910
                     /locus_tag="LP543_002208"
     CDS             10125..10910
                     /locus_tag="LP543_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HBL2322358.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10943..11326
                     /locus_tag="LP543_002209"
     CDS             10943..11326
                     /locus_tag="LP543_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBL2322359.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11402..11533
                     /locus_tag="LP543_002210"
     CDS             11402..11533
                     /locus_tag="LP543_002210"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL2322360.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11502..11738
                     /locus_tag="LP543_002211"
     CDS             11502..11738
                     /locus_tag="LP543_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL2322361.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11816..12787)
                     /locus_tag="LP543_002212"
     CDS             complement(11816..12787)
                     /locus_tag="LP543_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBL2322362.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         4056 a         2911 c         2306 g         4073 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     2761 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     2821 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     2881 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     2941 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     3001 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     3061 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     3121 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     3181 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     3241 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     3301 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     3361 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     3421 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     3481 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     3541 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     3601 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     3661 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     3721 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     3781 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     3841 gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt
     3901 gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc
     3961 aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat
     4021 ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt
     4081 gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa
     4141 catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg
     4201 aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc
     4261 tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat
     4321 tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc
     4381 ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg
     4441 aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa
     4501 gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt
     4561 ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt
     4621 ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag
     4681 tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc
     4741 agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa
     4801 gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg
     4861 cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt
     4921 gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat
     4981 caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa
     5041 acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat
     5101 gaatccggca ttcgtttagc cttagagttg ccttactcaa atggcaaaat agaaaatctg
     5161 catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt
     5221 tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa
     5281 ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg
     5341 tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat
     5401 tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt
     5461 caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt
     5521 gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc
     5581 aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc
     5641 gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc
     5701 aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg
     5761 cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt
     5821 cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg
     5881 ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc
     5941 agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac
     6001 atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg
     6061 aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt
     6121 catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg
     6181 cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc
     6241 cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag
     6301 ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag
     6361 attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt
     6421 cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag
     6481 cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct
     6541 agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg
     6601 accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa aaatcatcag
     6661 agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga
     6721 catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat
     6781 ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa
     6841 cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat
     6901 cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg
     6961 gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac
     7021 cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag
     7081 ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga
     7141 gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa
     7201 aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac
     7261 attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg
     7321 gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg
     7381 ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag
     7441 gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat
     7501 gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga
     7561 tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt caatccccac
     7621 caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc
     7681 cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg
     7741 gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa
     7801 ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca
     7861 aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg
     7921 ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat
     7981 gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc
     8041 tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa
     8101 acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg
     8161 aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg
     8221 catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa
     8281 ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg
     8341 cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct
     8401 gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct
     8461 cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca
     8521 aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg
     8581 tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat
     8641 ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata
     8701 acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct
     8761 caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta
     8821 gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa
     8881 agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc
     8941 gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg
     9001 cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat
     9061 tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac
     9121 ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg
     9181 tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca
     9241 agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag
     9301 taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat
     9361 acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc
     9421 tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag
     9481 gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga
     9541 atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga
     9601 ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac
     9661 ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt
     9721 tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca
     9781 atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc
     9841 gattgcttcg ttgcttcacc tgagacgcag cctgtcagca ggatacttac tgttgctgca
     9901 caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc
     9961 ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt
    10021 ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct
    10081 tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc
    10141 agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct
    10201 atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa
    10261 aagatgacat tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct
    10321 attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga
    10381 tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg
    10441 gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg
    10501 aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc
    10561 actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg
    10621 aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa
    10681 atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt
    10741 atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt
    10801 atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc
    10861 tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata
    10921 atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat
    10981 ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt
    11041 gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg
    11101 ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc
    11161 gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca
    11221 gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg
    11281 ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg
    11341 cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg
    11401 tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct
    11461 ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg
    11521 ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag
    11581 ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta
    11641 atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg
    11701 tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg
    11761 aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga
    11821 aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt
    11881 gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca
    11941 aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa
    12001 agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa
    12061 ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt
    12121 ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg
    12181 gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca
    12241 gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt
    12301 taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg
    12361 tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat
    12421 agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat
    12481 tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg
    12541 cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct
    12601 caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct
    12661 tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa
    12721 caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc
    12781 cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt
    12841 accatttaat cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa
    12901 aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct
    12961 aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta
    13021 gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt
    13081 gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa
    13141 aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt
    13201 accttaaatg attccattta ctttttatct cattttttcc tactagttag tatcaaatac
    13261 tctcctattt tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt
    13321 gtcattacag caaaaaatca cgttat
//
LOCUS       DAFEPQ010000071        13334 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.073, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000071
VERSION     DAFEPQ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.073"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(180..737)
                     /gene="frr"
                     /locus_tag="LP543_002213"
     CDS             complement(180..737)
                     /gene="frr"
                     /locus_tag="LP543_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBL2322363.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(740..1462)
                     /locus_tag="LP543_002214"
     CDS             complement(740..1462)
                     /locus_tag="LP543_002214"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBL2322364.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1598..2479)
                     /locus_tag="LP543_002215"
     CDS             complement(1598..2479)
                     /locus_tag="LP543_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBL2322365.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="LP543_002216"
     CDS             complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="LP543_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBL2322366.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3719..4198
                     /locus_tag="LP543_002217"
     CDS             3719..4198
                     /locus_tag="LP543_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBL2322367.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4415..5506
                     /gene="phnW"
                     /locus_tag="LP543_002218"
     CDS             4415..5506
                     /gene="phnW"
                     /locus_tag="LP543_002218"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBL2322368.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5499..5627
                     /locus_tag="LP543_002219"
     CDS             5499..5627
                     /locus_tag="LP543_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBL2322369.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5631..6176
                     /locus_tag="LP543_002220"
     CDS             5631..6176
                     /locus_tag="LP543_002220"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBL2322370.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6629..8320)
                     /locus_tag="LP543_002221"
     CDS             complement(6629..8320)
                     /locus_tag="LP543_002221"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBL2322371.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8740..9687)
                     /gene="arcC"
                     /locus_tag="LP543_002222"
     CDS             complement(8740..9687)
                     /gene="arcC"
                     /locus_tag="LP543_002222"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBL2322372.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9802..10821)
                     /gene="argF"
                     /locus_tag="LP543_002223"
     CDS             complement(9802..10821)
                     /gene="argF"
                     /locus_tag="LP543_002223"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBL2322373.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10912..12141)
                     /gene="arcA"
                     /locus_tag="LP543_002224"
     CDS             complement(10912..12141)
                     /gene="arcA"
                     /locus_tag="LP543_002224"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBL2322374.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12602..13303
                     /locus_tag="LP543_002225"
     CDS             12602..13303
                     /locus_tag="LP543_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL2322375.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         3751 a         2712 c         2367 g         4504 t
ORIGIN      
        1 acttaaaaga ttcccactat cttaagtatt atagcaatat ctaactgttt ttcctatgaa
       61 atcattgttt ttttatttaa aaactgaata attttttctt tttttaattt ttaaataaaa
      121 gaaatataaa taaaaacaga ggaagcggaa tgatccgccc ctctgttttt atgattttat
      181 taaacttcca ataattcttt ttctttatca gcagcgatct tatctacttc tttgatgctg
      241 ttatctgtca atgtttgtac gtctttttct aatccgcgca aatcatcttc ggtaatatca
      301 ccattttttt gttgtttttt atattcatcg atcgcatcac gacggatgtt acgaatcgcg
      361 atttttgcat tttctgcttc ttttttcaca tctttagcta attcttttct gcgttcttct
      421 gtcagttgcg ggataactaa gcgaatgaca tttccatcat tggttggact gattccgata
      481 tcacttgcca taatagcttt ttcaacatct tgaagaatgc tcttgtcaaa aggtgtgatc
      541 attaaaacac gtgcttctgg aatttggatt gatgccattt ggttcaatgg tgttggcgca
      601 ccgtagtagt tcacagtaat acggtccaaa aggctagcgt tcgcacggcc tgcgcggatt
      661 tgcccaagtt cacgttgaag attttgtgct gcttttttca ttttttcttt tgcttctgtc
      721 ataattgcat ctgccatttt tatttccccc ttacagttgt tccgatattt tcgcctaaga
      781 ttgcacggcg aatatttccg tgttcattta aattgaagac aagcagtgga atatcattat
      841 ccatgcttag tgaacttgca gttgaatcca tgacttgtaa tcctttggca ataacttcca
      901 gatgagtcag ttcttcaaat tttactgcat ttgcatctac tttcggatca gcagaataaa
      961 caccgtctac attgttttta gccatcagga taacatctgc gccgatttct gccgcgcgta
     1021 atgctgctgt tgtgtctgtc gagaagtacg ggttgccggt accgcctgca aagataacga
     1081 tccgtccttt ttctaaatga cgttctgctt tacggcgaat atatggctct gcgatttgac
     1141 gcatttcaat cgaagtttgc acgcgtgtcg gtacacccac gttttccaac gtatcctgta
     1201 aagctaaggc attcataact gtagctaaca tacccatgta atcagcttga gcacgttcca
     1261 tgcccatttg tgctccgatt tgtccgcgcc agatatttcc gccgcccaca acgattgcca
     1321 tttcaacgcc cagctcgtga acttctttga tttcttggat gatttctttg attactggtg
     1381 gtttgattcc aaatcctgcg tccccagcta aagcttctcc actcaatttt aagactacac
     1441 gctgatattt cggtgtgacc atgttcattc ctccaatatt ttctattgcc attttaccat
     1501 acttagatga tttcgacact ctaagactgg tttttcttgt tttgcttaaa aaaagggagc
     1561 gcacaaaccg tgcgttcccg tctgttattc aacagaatta ttttttcact tggctcataa
     1621 cttcttctac aaagttatct tcgcgttttt cgataccttc gccaacttcg aaacgtacga
     1681 atgttttaac agttgcgcct tttgatgcaa cgtatttttc aacagtcata tcaggatctt
     1741 taacgaatgg ttggtccacc aaagcgattt ctgctttgaa tttcttcaag cgtccttcta
     1801 ccattttttc aacgatattc gctggtttgc cttcgttcaa tgcttgttct gtaagaacag
     1861 ttttttcatg ttctaattca gcttctggaa tttgtgattc gtttacataa cgaggattga
     1921 tagctgctac gtgcatagct acgtctcttg caactgtttc gtcagttgtt ccatctagaa
     1981 cagtcaatac ggcaatacgt ccgcccatat gcaagtatcc accaaatgca gcattgtcgt
     2041 ctttttctac aacttcaaag cgacggaagc tgattttttc tccgataact tgagttgctt
     2101 cgatcaagtc agattcaatt gtacctttat ctgttttgat tttcattgca gcttccatgt
     2161 ctgctggttt gttttcagca acaagttctg caatttcttt tactaaatct tggaacattt
     2221 cgtttttaga aacaaagtct gtttctgagt ttacttcaac gattgctgca gtgttacctt
     2281 tcacagcaac tgaagccaat ccttcagctg caatgcggtc atttttcttc gcagcctttg
     2341 ccattccttt ttcacgtaaa agatcaactg ctttttccat gtcgccttct acttcaacta
     2401 atgctttttt cgcatccatc attcctacgc cagtcatttc gcgtagttct tttaccattt
     2461 tagctgtaac gtctgccatg tttaactcct cctgtgtgtt tttcttttta aaaaaagctg
     2521 cttcaaagga gagtgaggct tgtcgcccag cctttgaaac agctccatca ttcaaaatta
     2581 ttctgctgaa gaattgtcgc cttcaacaac atctacgatt tcttcgattg atgttgctgt
     2641 gttttcttct gtgaaatctt cttcaactac ttgatcttca ccttggtttc cttcgatgaa
     2701 agcatcagcc atttttgaag taatcaattt aacggcacga attgcatcgt cgtttgaagg
     2761 gattactacg tcgatttcat ctggatcgca gtttgtatca accatagcta cgattgggat
     2821 atttaatttg tgtgcttctt gaacagcaat acgttcttta cgtgggtcaa cgatgtacat
     2881 tacatctggg attcttggca tatcagcgat accgcctaag aatttttcaa gacgttcacg
     2941 ttctttgttc aaaccagcaa cttctttttt aggtagaact tcaaaagttc catcttcttc
     3001 cattgcattg atttgtttca aacggctgat acgtttttgg atagtatccc agtttgtcaa
     3061 tgttccgcct aaccaacgat ggttaacgaa gtattgtcct gcacgagttg cttcttcttt
     3121 gattgcttct tgtgcttgtt tttttgtacc tacgaataat gctacgccgc cttcttcagc
     3181 aacgctcttc atgtaatcat aagctgcatc tactaattta actgtttttt gcaagtcaat
     3241 gatgtagatt ccgtttcttt ctgtgaagat atatttcttc atttttggat tccagcgacg
     3301 agtttggtga ccaaagtgta cgccggcttc tagtaattgt ttcatagaaa ttactgccat
     3361 gtttgtttcc tccgatttgg ttttattttt cctcttctta tcttctacct tgccggcaaa
     3421 ctttttcaag cacctaccga caatccaata agaatgtgga tttgctgatg cataaagcac
     3481 cctcagctat tatacagaca aaagatccgt atcgcaagag caaatatact tttctgctca
     3541 acattcaata gtttacctga aagataaaaa gaattcaaga aaaacagtag atttctcttt
     3601 gtttctttat tatccttttt ccataaaata attttaggta taaaagacga atattattgg
     3661 gcaatccttc ttttttttgt tatgatacat gtgaaatctt tcgtaaagga gaaagaaaat
     3721 gcgcaaagct caaagacatt tgctgattaa acaagtcatt gaagaattcc cgatccgtac
     3781 gcaagaagaa ctattaaaca aacttgggca ttatggcgtt accgccacgc aggcaacgat
     3841 ttctcgagat atccgtgatc taaaaatcgt gaaggcacct gatgaaaatg gcgtttcccg
     3901 cttcgtattg tttcgcgaaa aaggaactcc tgagactagg actgaggaag aggatcggct
     3961 ggttcaaatg attgaagaca ttgtattgaa agtcgaacga gtccactttt tgacgatcgt
     4021 caataccttg ccagacaatg ctcatttatt tgcatctgtt ttagatgaga tcaaacctcc
     4081 ctctattgtc agtacaattg ctggattcga tacgatcatg gtcatctcgg aaaatgagga
     4141 ggacgcaaaa cgagtagaag aatttcttca ccatccaact gaagtatcac ttttttaatc
     4201 agagcacgta ttcctaatat ggaatgcgtg tttttttatt ttcaatattt tccttattga
     4261 ataaaccttt acactcttta caaaaataca tcatttttta ttcaaatttt tcaacacctt
     4321 gattgaatca caataatcat catttatatt ggatgttaac aagagcagct tcttgctctt
     4381 tgtgtatttc aaaaaagaaa tgaaaggaaa tcatatgtat aaattattaa cttctggacc
     4441 attgacgact tctgaaacag tgaaaaatgc tatgctgatc gatcattgta cttgggatca
     4501 agaatacaaa gaaattacac aatggataag aaaaaaatta ttggatcttg ctcaagtatc
     4561 ggaaggaata tacacaacgg tcttaatgca aggtagcagc tccttcgggg tagaatcggt
     4621 attaagttct gtgatcaggc cagaagatac tgtactgatt tgttcaaacg gcgcatatag
     4681 acaacgaatg atcaacatgt gcgaatgtct gaaactgaac tataccgttt atgaggtatc
     4741 ggaacatcaa atcctccaag ctcatgtgat tacacagctg attgaagaga atccgtctat
     4801 ctccgcacta gcaatggtcc atagcgagac tacttccggt attttgaata atttggaaga
     4861 gattgctgct gtcacacaag aaagagatct tttgtttata gtggacgcga tgtcaagctt
     4921 tggtgggata ccgatttgtg tagccgaact agggattgat tttttgattt ccagctcaaa
     4981 caaatgtatt caaggtgttc caggcttttc ttttgtactt gctaataaag cggaactgga
     5041 aaaacgaaaa aatgttagcc gaagtttgtc acttgatcta tatgaccagt accgcgtgat
     5101 ggaaaacgag aatggcaaat ggcgtttcac ttcccctacc catacggttt tggcttttca
     5161 acaagcgctg aaggaactag aggcagaagg aggaatcgaa actcgatatt cacgttatct
     5221 cgccaataat catgtgctac gacaaaaaat gacagagatg gggttccagc ctttcattca
     5281 agaatatcag gggccattca tcacctcttt tctttaccct aaggactggc gatttgattt
     5341 taatcatttt tatcagtatt taaagcaaaa tggatttgtg atctatcccg ggaaaatcag
     5401 cgatcatgaa tgttttcgta tcggaaatat tggcgagatc tatccggaag atattttgga
     5461 actgacagca attatcaaag actataagga gaacgttaat gattaaaact gtaatttttg
     5521 attgggcagg aacaactgtg gactttggct gtatggctcc tgtccacgct tttagaaatg
     5581 cttttctaga aaaaggaacc caactgactg acaaagaaat ccgctaaccg atgggaaaat
     5641 taaaatggga tcatatccaa cagcttcttt ccctcccttc tgtcaaaaaa caatggatag
     5701 ctatttatgg acaactacct caggaaaaag aggtggagga attgtatcaa caatttgaac
     5761 attatctgtt taaagatttg gtcaagcatt ctaccttgaa acccgacaca ttggaaacga
     5821 tcgaacggtt gagaaacgct gggatcaata ttggttccac gaatggattt aatgctccta
     5881 tgatgacaat cgtagcagaa accgcaaaaa aagaaaggta ttcaccagac ttcatggcaa
     5941 cttttgaaga cgtcgaaggg tatggtcgac cgtttcctta tatgatccat aaaaacatgc
     6001 agtacttcaa aaacaagtcg gttgatgaag taatcaaagt cggagatacg gtctcagata
     6061 tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt aattgaaggt agttcgctaa
     6121 tggggatttc ttatgatgag taccaaaaat tagagatggg gaaaaagatc gtttaaaaca
     6181 gtctgttgct cttagattcc aacaagccgg cgctgatatt tgtgtaaaca atttaaaaga
     6241 gttaacagaa tacattttgt acgctgacaa gaatccagaa tgacttttat acaaaataag
     6301 aggttgtgaa aaagctgtcc tgtatcaagt aataaaaaca gccaagcaaa aatagctcct
     6361 catatttttc gtttggttag gcttattacc gcagatgcaa gcttttgaac accgtttatt
     6421 cgaaaaaaaa agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac
     6481 agtgtttaag agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg
     6541 ctcaaaaaac gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc
     6601 agaacgctga tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca
     6661 aacgaaggtc ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt
     6721 tttgttcatc atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta
     6781 atttgattgc atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg
     6841 tttcagggta tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat
     6901 agtctgctgt ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat
     6961 gtgtatattg cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa
     7021 agtcaaatgt attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa
     7081 cttgtttagc tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt
     7141 tagcagattg gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag
     7201 ttgataattt tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag
     7261 accagtcaaa gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt
     7321 cattccctac tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag
     7381 cagctaagtc acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat
     7441 tcaggtcata agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt
     7501 tttcttctaa aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca
     7561 acgaatcgaa acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat
     7621 cacggaacca ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc
     7681 aagcacgagc ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt
     7741 ataaacgaag caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt
     7801 tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga
     7861 ttggctgata accgattttt tccataatga aaccgatcga attaccgata actgtcgaac
     7921 gcaaatgtcc cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg
     7981 ttccttgatt cccgatgttg ctatccccat aatgttcttt ttctttcact actgtttgca
     8041 aaactttttt gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt
     8101 cgattttttc aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg
     8161 gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag
     8221 ctgattttgg attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag
     8281 cgtcatagac agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt
     8341 tcccttgatt gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta
     8401 gagacgagat ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga
     8461 tatcggtaga cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc
     8521 ttcattcggc ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg
     8581 ttcaacaaat cttagtctta ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg
     8641 ctagtcgttt cataaaaaaa aaatccagat ggcctaaagg agaagaaata ccacctggat
     8701 agctataaat catttcgtaa atacgaaaga cttaacaaat taatcagcta cgataatcgt
     8761 tccttcttca gaagctaaaa gattctcgat attttctaaa gaagtgatga ttgccttagc
     8821 gtttggtttt gcttcaacaa atgcaatagc tgcttctact ttcggaagca tgcttcctgg
     8881 agcgaattgc ttttcatcaa tgtattgttt catttctgaa acagtaacag tttctaattt
     8941 cttttgatct ggtttttggt aattgacata cacattgtct actcctgtca atacgatcag
     9001 caaatccgca tcgatgattt cggctaattt ttctgaagca aaatctttat cgattacggc
     9061 ttctcgtcct tctaatccag tagctgtttc tacaacagga atcccaccgc caccaacaga
     9121 gactgtgata actccttgat cgaccagcgt ttcgatgaca cgtgcttctt taatcgagat
     9181 gggtttaggg gaagcgacta cttttctcca tccacgtcca gcatcttctt tgaatgtcac
     9241 agtggagtca gcattcattt gttctttggc ttcttcttcc gtgtagaatg gaccgactgg
     9301 tttacttgga tttttgaatg aaggatcatt ttcatccaca ataacttgag tgactaaaga
     9361 aaccacatct ttatcgatcc ctttttcctt cagtacttcg cccatggcgt tttgtaacca
     9421 gtaaccgata gatccttctg tcattgcaac acatgtatct aaaggcattg ccggtgtttt
     9481 ctcagaatca gcagcttgct gctgaatcaa taggttaccg acttgcggac catttccgtg
     9541 ggagatgatc aattcatctc cttgttcgat aaatttcact aaatactttg ctgtttccat
     9601 taacgcttct tgttgggctt ttgcacttgc atccgtagac aagatggcat tgccccctaa
     9661 agcgacaact acttttcgtt tggccatttc attcgtcctt tcttcagttc taccgaattt
     9721 tctagataac aaaacgagcc agcgttttgt gattttccct caatgctgac tcgttttttc
     9781 gtgagatgaa tgtggttatg cttaaacgcg agggataaac aagttaccta atgttgctgc
     9841 catgatcgct ttgattgagt gcatacgatt ttctgcttgg tcaaattgac gagcatattt
     9901 gctgcggaag acttcatctg ttacttccat ttcagtgatg ccaaagcgtt ctttcatttg
     9961 ttcaccgtaa acagtgtttg tatcatggaa agctggcaag cagtgcaaga agatcaaacg
    10021 atctgtgttg tgtgtttttt ctaccatttc catattgatt tggtaaggtt tcaacaattt
    10081 gatacgttct tcgaatttat cttcttcacc cattgatacc catacatcag aataaaggac
    10141 gtctgctccg tctacacctt tatcaacatc gtcggtaatc atcaattgtg caccggattt
    10201 ttcagcaaag ccttctgcca ttttgacgat ttcttcttca ggttgtaatt cttttggtgc
    10261 aacgatacgc atattcgctc ctaaaattgc acctgtgact aataaagagt tagccatatt
    10321 gtttcgacca tctccgcagt aagcaacagt gataccttct actgttccaa agttttcttg
    10381 gatagttaaa aagtcagcga tcatttgtgt tggatgccat tcatcagtca agccattcca
    10441 aactggaaca ccagagaatt ctgctagttc ctctaccatt ttttgactga atccacggaa
    10501 ttcgattccg tcaaacatac gtcctagtac tttagctgta tcttctgtgg attctttttt
    10561 acctaattgg atatcatttg cacctagata ttcaggatgt gcacctagat cgatcgctgc
    10621 agtagtaaat gcagaacgag tacgtgtaga tgttttttca aataaaagcg caatattttt
    10681 tccttctaga tagtgatgtg ggataccacg ttttttcaag tctttcaagt gttctgaaaa
    10741 atcgatcaaa tattgtaatt cttcttttgt aaagtctttt tctgctaata agcttcttcc
    10801 ttggaatact gattctttca tattaaacat tctcctttag attgttttta tttaagatta
    10861 aagaggaaat cccgacggca gctgacagta agactgccgt caggaaacaa attacttcaa
    10921 gtcttcacgg acaagtggtt ggctcatgca gcgtggacca ccacgacctc gtgacaattc
    10981 acttgaattg atttctaaca ctttgatgcc atagctacga agcaattcgt tcgatacata
    11041 atttcggtta taagtgacta caacacctgg agcgattgcc aatgtgtttg atccatcatt
    11101 ccattgttct cgaggtgcaa cgatctcatc tccattacca gttgggatca gtacaagatc
    11161 atctaatcca agagcagctt tcatcgtttc gtgtaagtta tctgaatgag taatcttgat
    11221 atcatcacca tctggttcga ttgtaaagac atccatctta ccgtctttac tttgaattgc
    11281 tggatggata gtgaatttat cgtgatctac cattgtaaat acggtatcca aatgcatcat
    11341 tgcacggttg ttcggaattt tgattgccaa cactttttca aatcctgaat tttttgcgaa
    11401 taggtggcgt gctaattttt ctaaagcttt cgcattagtt cgttgagaaa tcccaacagc
    11461 tactacttta tcgcttaaga tcaattcgtc tccgccttcg atatgatctg gattttcacg
    11521 atccaaccat acttcgattc ctttatttgc aaatcttgga tgatgtttca aaatatattc
    11581 tgtaaacatc gattcacgac gacgtgcttc aaaagtcatc ttattgactg tcatcccatt
    11641 tcccattgat gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt ccatgtaaaa
    11701 tggatagtca tcgtctgctg acagatcata tagactgcta gaacgtactt cgatatcttt
    11761 tgtacgaacc ccagccatga ttttatctac catttcttgc gtttccattg atagaaggaa
    11821 ctcatgaaga ccctctctta cagcattcga tgcaatatga gattcatcca acattttatt
    11881 caagaattgt tctttgacat tgcctgcgtc aatcgcttct gccgctaatt tttccaagta
    11941 aagagtctcg acgccttcat tttgcaatgt cttcgcaaag ttgtcatgtt cttcttgagc
    12001 aatcggaaga tatggaatat catcgaacaa cagacgatcc ataatatctg gtgtcaaatt
    12061 ttccacttct tgtccaggcc gtttcagtaa aactgtcttt aatttcccta tttcagagaa
    12121 aacgtgaata ggtttatcca tgttgtgttg cctccttctt tttgattaca tgtataggat
    12181 atcctccttt tgtaatcact ttcataattt tgtctcattc aaaatgagac attcctcaca
    12241 ttaatattcg ataattaaat aattaaacgt ttttctattt ttaagcgtaa tttattcatt
    12301 ttttattctg tatacacttt caaaaaaata tttattcatt gaaaaaattt ttttgaataa
    12361 attaaaaaaa taaattatat tatcgttgtt agcgtttact atatgtatta ttgttgtttt
    12421 ttatacacaa atcattcgct tcaaaacaaa ttattcaata ccattgtata ttttttacat
    12481 aataacaaat tatatttgta aagtattaaa acttgtttct aatgttggta tgttaaaact
    12541 ttacaaattc tttaaaaaaa gtgacaataa atatagtgaa caataaggag aggacttttt
    12601 aatgcgtgtt aataaaatcg attttcactt ttcaaaactc agatatcaac ctgattttga
    12661 acatttctcg gatgaagagt tccaacagat caaggaacat actgttcttc gttcttataa
    12721 aaaaggacag atccttttcg acgatggtga tcccagacaa cggttgtata tcttgaccaa
    12781 aggactcgtt cgtttagaac gttatgatga aagtgcaact tattactact atgactatgt
    12841 gaatacaaat aacctttttc ctatgtcagg aatgtttgga gaagagacct acacttatac
    12901 agctcaagcg atgactgata ttgaaacatt ctaccttcct gtttcctact atgaaaaatt
    12961 agcacgaaaa aatcccgaac agttgatcta tatgatacaa aaaatttcta aagtagttga
    13021 aatacatgaa ttgcgtatcc aaatcggatt gacctctagc gcctttgacc gggtaaaaca
    13081 aaatttattc attttgaaag aagaattggg cgtaccaaca aaagatggtc atatcaccat
    13141 tccttatccg atcaccttaa aagaattagc agtgaatagc ggcacgacac gtgaaactgc
    13201 tggacaagta atcaaacaac tacgtgattc cgggctgtta gattatgaca aaaagcgttt
    13261 tactttctgt aagacagcta ctactccagc tatgagagaa taacgcgaac aaaaataagc
    13321 agtgaccctg ataa
//
LOCUS       DAFEPQ010000072        13296 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.074, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000072
VERSION     DAFEPQ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.074"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            214..2919
                     /locus_tag="LP543_002226"
     CDS             214..2919
                     /locus_tag="LP543_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBL2322376.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPILQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            3516..5339
                     /locus_tag="LP543_002227"
     CDS             3516..5339
                     /locus_tag="LP543_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBL2322377.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(5481..5666)
                     /locus_tag="LP543_002228"
     CDS             complement(5481..5666)
                     /locus_tag="LP543_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL2322378.1"
                     /translation="MALNYGNKKWSLDEAEISTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            6141..6404
                     /locus_tag="LP543_002229"
     CDS             6141..6404
                     /locus_tag="LP543_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL2322379.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSGFTNIDDIDWEAL"
     gene            6404..6670
                     /locus_tag="LP543_002230"
     CDS             6404..6670
                     /locus_tag="LP543_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBL2322380.1"
                     /translation="MSNYTVAIKNSATVDLRKIKQSNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            6871..7705
                     /locus_tag="LP543_002231"
                     /pseudo
     CDS             6871..7705
                     /locus_tag="LP543_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016619675.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7901..8572)
                     /locus_tag="LP543_002232"
     CDS             complement(7901..8572)
                     /locus_tag="LP543_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2322381.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATDGTSIILAGALPTALMVIVTDWLLGILERRLDPA
                     SRTSR"
     gene            complement(8569..9486)
                     /locus_tag="LP543_002233"
     CDS             complement(8569..9486)
                     /locus_tag="LP543_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL2322382.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYDQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVNNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            complement(9483..10124)
                     /locus_tag="LP543_002234"
     CDS             complement(9483..10124)
                     /locus_tag="LP543_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2322383.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            complement(10128..11303)
                     /locus_tag="LP543_002235"
     CDS             complement(10128..11303)
                     /locus_tag="LP543_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL2322384.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESDTPEESKEV"
     gene            11496..12527
                     /gene="queA"
                     /locus_tag="LP543_002236"
     CDS             11496..12527
                     /gene="queA"
                     /locus_tag="LP543_002236"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBL2322385.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKIGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            12721..13296
                     /locus_tag="LP543_002237"
                     /pseudo
     CDS             12721..13296
                     /locus_tag="LP543_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4188 a         2517 c         2758 g         3833 t
ORIGIN      
        1 gataaaaact ttggcataaa attatctttc ccttttattt catgataaag taaagtctgt
       61 aatccttaat tatttctcaa tatgtaagag attacaatga gacaattgcc gtgatatgtc
      121 caaaaattcg acttttttga aaaaagccga ttgaaaaaag tatgaaatac atggtaaagt
      181 aattcagctg aaaaaagaag aagggggaga actatgcagt caatcaaaaa tacgcttcga
      241 ttatatttgc ttgactggaa acggattttc aaaaacccga ttgcaacact tctgatcatc
      301 gcgatcatga tcatcccttc gctatatgcg tggtttaaca tcaaagcatt atgggatcca
      361 tatggaaata ctggagagct gccaattgct gtttacagtg cagacaagcc agcagagttt
      421 caaggtaaag aagtggccat tggggagcaa gtcatcgaat cgcttcataa aaataaacaa
      481 ttaggatggc aattcgttga ttcaaaagaa caattagaag acggagtccg ttccggaaag
      541 tattacgcag gtatttactt gccaaaggat ttctcagaag atctgctgag ttttactagt
      601 ggtgatatca agaaaccaaa aatcgaatat acggtaaatg aaaaaatcaa tgcgattgca
      661 ccgaaaatca ccgataaagg agcaagttcg atccaatcgc aaatcacaaa tgaatttatc
      721 aaaacagcca gttcgacttt attgaaagta ttcaatgaga taggttacga catcgatacg
      781 aacctagtaa gtatcaataa agtcaaagat atgattttat caacagatga aaatttggac
      841 acgatcgacg gctacacaaa acaagtcctc gaattacaaa gtcaactacc tgaaatcaag
      901 gaaaaactga ataaagcaaa tgaattcgtg gattatatcc ctaaagtaga tgagatgggt
      961 gaaaaagtcg ttgcattgaa tgacaaaatg cctgagttga aagatcaagc aaaaatcatc
     1021 cttgatttgc aagaaaaaat cccagagatc caaaatgccg gtaaacaact agcagagatc
     1081 gacggcgatt ttgcttcgat ccaagagaca atgaaccaag gaatcgatga agcaaaacaa
     1141 ggcttgacga tcatccaaca agtacaggat atcttgccag atgtgaaaaa tttagaaaat
     1201 caggcaagtg atctagcaaa ccaaacaaaa tcaggtgcct ctcagctgaa agatgcgata
     1261 ccaagcatca caaacagtgt agatgttaca ttgaaatcaa ttggacaagt ggcaacaacg
     1321 acaggttcgt taatagatac tgtaagaaca gctattgaag atggtaagct gactgatgat
     1381 gaaaagaaaa acatcagcaa tgtattgaac ggctttattg acggaatatc tcaacaacaa
     1441 aatgcaatcg ataaactggt cgagttcctg actaaattgc aaacaagtgc tggaaatacg
     1501 aaattgcagc caattattga tcagctaaat caattaaaac ccttgctaag caatctgcaa
     1561 aaccgcctat ctgctttgaa cacagctgtt caaaatgaag acacgaaggc tattgatgaa
     1621 ttgctagatc aaattgatgc cgttgttaat aatatcaatg gcatcattgc tggtatcaat
     1681 acaggtcaaa tcagttccac tgtcacttct ctattagacc aagtcatcga tacgattggg
     1741 actgcgcaag gggcgctaag caaagcggac cagatcgact ttgattcgct tctcagttca
     1801 acgaaagcaa ctgtaacgaa tgcagtggca attttagaaa aatatcaaaa agaaatgcct
     1861 gcaattggac aagaaatcca tgatgccaat acaatgctga atggaaatat ggataccatc
     1921 gtcaatggca tcaatgaagg ggcagaccta tacaacaatg aattaccgat ccttcagcaa
     1981 aaattaggat tggccgctga ttttatccaa aatgattggc cagcactaaa aaatgaaatc
     2041 accggtacat tgacaacagt aaatgaaaaa atgccagaag tcgaaacagc tgtgaacgct
     2101 gctgcggact tgatcaacaa tgactggcca aatatcaaag ctggtatcca aaaagcagca
     2161 gacgcgatcc gaaaaggcga acaagaagtc gatctaggcg aagtcatcaa attattgaaa
     2221 ctagatgcaa caaaagaaag tgacttcttt acacaacctg ttgagctaca aaccaatgcg
     2281 ctttatccaa tcgcaaataa cggttcagct agcaccccgt tttatactgc actatgtctg
     2341 tgggtcggcg ctgttcttct ttcaagtgtt gctacgacag aatactattt aggaaagaaa
     2401 gaccgaaaca attttacgaa acgagaacaa ttcgttgctc gtatgctgac atttttgaca
     2461 atggcgattg cgcaaagctt gatcgttaca ttagggaata ttttcttact aggagtagat
     2521 gtccaaaatc cggtctacag cgtgtcattt gctgtcttga tcgccttagc gttcatgatg
     2581 atcgtctatg tcttggctgc attattcgat aatgtcggta aagggatcgc tattatcatc
     2641 ttagtactgt cgatctctgg cggaggcggg aactatccga tccaagtctc agggaaattc
     2701 ttccaaatga tcaatccgtt cttgccattt actcatgcgg taaacttgtt acgggaatct
     2761 gcaggaggca tttactggcc aaatgctacg aaagcaatct ggatcatgat cggtctgttt
     2821 gttgtctttg gaatcgtagg aacggcaatc tatccgttca tcgaagaaaa aatgacgaaa
     2881 ttcaaagaag cggcgcatga gagccatatt tttcattaaa aaaagaggtt gtgaaatcag
     2941 cgttttgacc tgagtattag agaaaaaaat ggaaaaatag ttcctcatat tttgccgttt
     3001 tttgagctaa tgccgagggt cagctgattg aacaccgtgt accaagaggt tgtgaaaagg
     3061 ctgttctgca tcaagtaata agaacgacca aataaaaaca gcttcttatg tttttaattt
     3121 ggttgggctt attaccgcag atgcaagctt ttaaacaccg tttatcaaga ggttgtgaca
     3181 actgcgctat gcttcgagta gtaagaatga tgggtagaaa gcaaccttac atctctcttt
     3241 caaaagttaa gatatcaaaa aggatagaac ttgattttct aagtcaagtt ctatcctttt
     3301 attatagaat tcaaagaaaa agcccataaa aaaatgttag atttttgctg tattaaagcg
     3361 tttgaaaaag cgcttgatat ttttctgttt ttttccaatt attttcttaa agtttattga
     3421 cttttaaggt cagtgagcgt actataacat cttgtttact ataaattttc gactataaaa
     3481 cttgaagtaa aattgtgtgt aaaggagttt agaaattgga ttacttaaaa aggttgttag
     3541 taggtaagcc tttaaaatct gctgaaaatg atgagcacaa attaacccga tttgctgctt
     3601 tggcattatt gtcgtctgat gcgttatcat ctattgcata tggtacggaa caaatcgttg
     3661 tcgttttagt cgctttgtct gctgcagcca tctggtattc gctacccatt gccgcttttg
     3721 ttattatttt attgatttca ttaacgctat catatcgtca aatcatccat gcttatcctc
     3781 atggcggggg agcatatgtc gtcagcagtg agaatcttgg aaagaatgct ggcttgattt
     3841 ccggtggctc tttattgatc gactacatgt tgacagtagc agtatctgtc tcagcaggtg
     3901 ctgaagcgat cacatcagcg gtgccagcac tctacggtca tcaagtcgct atttctgtga
     3961 caatcgtttt gctactgatg atgctgaatc ttcgtgggct gagggaatct gcatcgttcc
     4021 tattatttcc ggtgtacaca tttatccttg tgatctcgtt actgatcgtt gttggtttat
     4081 ataatatcgt gacaggtgct gtgccattgc aagctactgc acttccagga gcggctgtgc
     4141 cgggtgtttc tatcgctttg attttacgag cattttcttc tggttcttcc tctttaactg
     4201 gggtggaggc gattagtaat gctgtaccat tctttaagaa gccaagagca aaaaatgccg
     4261 ctgctacatt gacgatgatg gcattgatct taggtttctt cttcgtgggg atcacattta
     4321 tcaactattg gtatgggatc gttcctgaaa aagaagtcac tgttttgtct caaattggta
     4381 aagctgtctt tggacatggt attttgtatt acgtccttca atttgctaca gcgctgattt
     4441 tagcagttgc tgctaacacc ggtttttctg cttttccagt tcttgcatac aatctggcaa
     4501 aagataaatt catgcctcac atgtatcaag atcgtgggga tcgactaggc tattccaatg
     4561 ggattatcac actagcgcta ggttcgatcg tattgctgtt tattttccat ggctcaacag
     4621 aacgcttgat tccgttatac tctatcggcg tgttcattcc atttgcttta tctcaaacag
     4681 ggatggtcgt caaatggaaa aaagaaggaa aaagatggtt aagcaagtcg attgccaata
     4741 ttacaggtgc gttcatctca tatgcaatca ttgcgatttt gttcgtctat cggttagggg
     4801 acatttggcc tttcttcatc attatgccaa tcgttatgtt cattttttat aagatccatg
     4861 atcattataa aaaagtagca gaacagcttc gtttagaaaa tgatgcaaaa ctacatgatt
     4921 atgacgggaa taccgttctt gtattagtcg ggaatgtgac acgggtaaat atcggtgcgt
     4981 tgaattacgc acgttcgatc ggtgattatg tcgttgcgat gcatgtttcg ctagacgaag
     5041 atgtcgaaaa agaaaaagaa atcgaagcag aatttaaaaa gcatttccca gatgtccgtt
     5101 tttcaatcgt ccactcttct tatcgctcga ttgaaaatcc aattatccgt tatgtagata
     5161 tcgtaagtaa aaatgcagcg aaacaaaact atacaactac tgtgttgatt cctcaatttg
     5221 taccaaacag acgctggcag aatattctcc ataaccaaac aagtttgcgg ttaagattgc
     5281 gattgtcttg gcgtgaaaat atcgttgtca gcacctatag ctaccatttg aagaaataaa
     5341 ccaagtttta aaatcaaaaa cagaccctct agctcttaat tgaagctaga gggtctgttt
     5401 tgtcttattc ccagctaaag gtgccctcta taagccatta aagatgggaa cagcgtatga
     5461 aatgttatta ttcttcctat ttagctgtaa aacattttga ttttgatgtc ttgatcataa
     5521 tttccgaatc ctttgccaaa taatgtacag cctcctatat tttcagcttc ttcctttacc
     5581 tcgactctta agtgccatgt gtcttcataa gtagaaatct cagcctcgtc aagtgaccat
     5641 tttttgttac cataattcag tgccattttt aattggtgat tcacaaaccc gacggtctaa
     5701 gcaagtatca aatcgatagt gaacatgaat ggcaggagac gaaagaggca atcatttttg
     5761 aggcagtaga agacttgacg gccaaaatca gttttgaata accgttacaa atctttcgtg
     5821 ggtatcctca ggttattcaa gatatcaatc aagtcacact gcaacgagga accttcgtgt
     5881 attggtgttg ttgcatagac tcacaggctt tggattcgac ctttgctaaa gatgatacgt
     5941 tgttaccaat tactttacgt ttggctaccg ctagtcgtaa aggagtaaga agtgagcgtg
     6001 gacaaagatc ctccctaaaa aggggtcttt ttctctatgt aaaaaagagg aaaagtttgc
     6061 tgaatttaag tgctttattg tattttttga cgtacttaat caagtacgtt ataattatat
     6121 cagaaaaagg aggagagaca atggctaata caacgatgaa tcctagcaca gctcgtaaaa
     6181 atttttatca gttattgaaa gaggtaaatg aaaatcatac tgaaatcgag attattagtg
     6241 atcgtagtgg aaacaatgct gtattgattg ggctagaaga ctggagagcg attcaagaaa
     6301 ccctttttct tgagcaaaca ggtactttag ataaagtacg agaccgcgaa aaagacgaca
     6361 gtggctttac gaatatcgat gatattgatt gggaagctct ttgatgagta actatacagt
     6421 cgcaatcaaa aactctgcga cagtggattt aagaaaaatc aagcaatcga atttaaagaa
     6481 acaatttgaa gaagtgattc agacattgaa ggaagatcca tatatgccca cgcagtcttt
     6541 tgagaagctg agacctacac atgaaggcag atactcacgc aggctgaata gacagcatcg
     6601 agtggtttac aaagttgatg aggaaaataa agttgtggaa atttattctg cctggacaca
     6661 ttatgagtga agtgagaaga gccaaccttt ttagatagtt aacaaagagt ctccccaaaa
     6721 aggagactct ttttttgttc taaaaccaac cttcttggat ttattgcatg atttctttaa
     6781 tttgttccta tatctccacc tttgaagggt cattgtttga gtttaggaat ggaatgttga
     6841 cgggtttatg taaaacaagg aggaagaagt gtgacaagga aaatcagtaa aattggagca
     6901 tcgttgttag tgttggcagt ggcaacgacc atcgattatc gccaaagtga ggcgaagaca
     6961 attggaaatg tcgtttatcg agaaacagcc aaaagtatgt tgaaaaaagc aatggacaat
     7021 caaccggagt cgtcttattg gttcccagag gattcgttgg catggtcttt tggaaaagat
     7081 acagatacga aatacaatac cagtgtcgtt tctttagcag agcgggtatc caaagcgaca
     7141 ttgccgaaca tgaatgtcac tcaaacggaa gaagtcaaag tcgtggcgct ttcggccatg
     7201 aatagcagta tcaacggcaa tgcatcacga gggatcgata tcttttacgc caatgtcttt
     7261 tcttattggc aatatatcga tcaattggtt tacgggggtg ctcttctggt gaaggatcat
     7321 cgtaccgcca agtctagttg cgacggatgc agtccataag aatcatgtac cggttttagg
     7381 aaccgcaaac ctcccatggc gagaagctgg aatggctaga tatattttta gccaaggatg
     7441 aggatggcaa gttcccgatt gtataaaaga tgatcgaagt taccgatacg gcagtcacca
     7501 gttttgatga tgtagctgaa ggcatcgaac aatcgaggac tgatggaaaa ggcttaaatg
     7561 aaagccacgc caaaggatgt aagaattgat gcccaattca aagaacaagc agacgagtat
     7621 ttagagatca tgtggtatga attccatgac gagtgatggg aaaatggatt ggcaaaatgc
     7681 cttagcggat aagaaaaaaa cttagtcggt ggatgcagag atgaacccgt tggcagtatg
     7741 aagttggagt atacggaatg gataaatagc taagaaaagc aactctttgc ccataaggag
     7801 ggaagaatca gagtggatta ttgccttaaa aaatgacaga actaaaaaag cctttgcgac
     7861 caacaaatgg tcgcagaagc ttttgttttt tacaataaca tcagcgtgat gtacggctgg
     7921 ctggatctag ccgtctttcc aaaataccta aaagccagtc cgtgacaatc accattaatg
     7981 cagttggcaa tgcacctgct aaaataatcg atgtgccatc tgttgcattg gttcctcgga
     8041 taatgatatc tccaagacca ccagcaccga cgaatgcacc gatcgcggtt atcccgatgg
     8101 caacaactaa cgcgtttctt atgcctgcca tgataacaga aacagagagt ggtaactcta
     8161 ccatgaatag tcgttgtcta gcagtcatcc ccatgccttt gccgacatca aggatgtttt
     8221 tgtctacttg gatcatcccg gtatacgtat tcttgatgat cggtaagagg gaatacaaaa
     8281 atacagtaac aatcacgaca ttgactccca ggcctaatcc aatgattaaa atggagatca
     8341 tggctagaga agggatcgtc tggatgatat tcgccaaacg aatcacccag tttgctaatt
     8401 tacgtctcct agaaatcatg atcccaaccg gaatcccgac aactgctgca aagaggacac
     8461 catagataga aatcaggaag tgacggataa attgtgcaaa gatataggag ccattttcct
     8521 ggaaataata gaaaaattgt tgaaagacat tcattttatc caattgcttc acttcccttc
     8581 aaaatagtgg ttttcttgta agaaacgttg tgctactact gatggttcca ctagattatt
     8641 atctgcttcg tagttcaatt cctgcatttt ttccgtactg atggtatgac ctaacttatt
     8701 taaggcttct tttaaatgag gcgtatcagc taatattttg ttgtttacaa ctggagcagc
     8761 atcatatggt gggaagaatt gacgatcatc ttccaacatg actagatcat aactggcgat
     8821 ccgtccatcc gtagagtagc caagaacaat atccattttt cctgcttcga ctgcgtcata
     8881 aacaagtcca atctgcatag gtaatatcga attgaaggag aagccataag tttcttggaa
     8941 gccgtcataa ccgtctcctt tacgattgat ccaagaagta tctacaccag ctactaattc
     9001 atccgctaca tttttcagat cgctgacttt agataaatgg tatttttgtg cggtgtcttt
     9061 tcgaacaaga aagacatacg tattttcaaa accataggac gggaaccacg tttgatcgta
     9121 tcttttctca aattcatctt tcacggtagc aaacgctttt ttcggatctt tttcaggagg
     9181 aaggttcaat gtagtcgtta aatctgttcc tgtgtagcga gccgctgaaa ttgaagcatc
     9241 tccgttcatc atcgcttgat ggttgatagt tgtggtagcc agattgttga tgattgcggt
     9301 atttttatct gtataatgtt caaccattcc ggcaactagg cttgctaaga tctgtgcttc
     9361 tgaagtgatt ccacctgtga tcgaaatggt gtcttcatct gtattgctgg caagaccagg
     9421 gaaagaacaa ctcgcaagta taagaagaga gaaaagaacc atggtcattt ttagtatacg
     9481 tttcattctt cattctcctc tctaagtgcg atcggtgtca gccggtgttc caagagacct
     9541 agaagccaat cagccaatag tgctaaaatc gttacaggga tcgtacctgc caaaatgaga
     9601 tctggttggt aattattcag tccactaaaa atcaaatcgc ctaaaccacc agcgccgata
     9661 taagaggcaa gtgtagccca tgcaatcacg taaacggcag ctaaacgaat accagccatg
     9721 atcgttggca tagcaatggg caattcaacg gaaaaaatgg attggaactc agtcatcccc
     9781 atgccttttg ccacatcccg ataattccaa tcgacatttt tcatcccaat gtacgtatta
     9841 cgtaaaatcg gcaataatga ataaataaag agtgcgataa tcgcaggtaa tttacctacg
     9901 ccaaatagcg ggatcatcaa tgccaataaa gcaagagaag ggattgtctg caaggcactg
     9961 gttaagccaa tcacaatcgt ggcagctttt ggaaaacgtg tcagtaagac accaagcggt
    10021 acagcaatga tgataccaat gatcaaagca agtcccgaaa tataaagatg ttcaaaacct
    10081 tttgagagaa tttctgaacc gtgctgatga aaaaactgct gcataggtca gacctcctta
    10141 gattcttcag gagtatctga ctctgcaaca tcagtagttg cgggatcttc tccccaaatc
    10201 acatcgtaga cgatatccac taatgatgca cgagtcagga tacctacgac tttgttctgt
    10261 tcatcaacga ccggcacgta tttcagtccg cgttttaaaa tgcgttgcaa ggtatcacga
    10321 agataggcgg attcttttac gtaaaaaaca tgtttattta atatgtcgcc gacactggat
    10381 acttttcctc gctttttatt caacgtttct acatcgataa atccttttaa tacattcgaa
    10441 ttatcggtaa ctaacaacgt atctacacgt ttttccctca tcagtttgat tgcttcttgt
    10501 aatgattttt ccggtgtaat cgtgattgct gtatttagca taacttctcc gactcttgtc
    10561 gcatttggct tggcttgtag taggcgatct tctccaatca actcttctac gaaatcgttt
    10621 gcaggatgac gtaagatatt ttctggtgta tcaaattgga tcacacggcc ttcactcatg
    10681 atggcgatac ggcttgctag cttaatcgct tcatccatgt catgggttac aaatacgatc
    10741 gtttttccca agcgctcttg taaatctttg acaaggtctt gcaaagaatc acgagtgatg
    10801 ggatcaagcg caccgaatgg ctcgtccatc aagatgatat cttgattcgc tgcaagggca
    10861 cgaacgactc caatacgctg ctgctgccca ccagataatt catgtgggta gcgatctaac
    10921 atctcacggg gcaattccac aagatcaatc attttttcag ctgttttatt ccgttcttct
    10981 tgactgactt tcaacaattt ttggacaagg gtgatatttt cacggatcgt catgtgaggc
    11041 atcaatccaa tactttggat aacatagccg atctgacgtc gcaattccac tggatcaatt
    11101 tcttggatgg attttccatc aatcaaaatt ttcccttgtg ttggatcagt cattcggttg
    11161 atcatccgca tcgtcgtcgt ttttccgctt ccgctcgtac cgataaaaca aatgaactca
    11221 cccttttcaa atgaaagatt gacgtcctct acagcgactt ttccaccttt ataaattttt
    11281 gacacatttt gaaattcaat catggtgcca cctctttttc tattttcaat gaagaactat
    11341 ctattactag tatgcctaaa acaatgaaaa aatacaaata gggtagtttt tttatttagg
    11401 aaaacaaaaa atagaagtga aaaagtgctg attttgccaa cagaatgaaa aacatgatag
    11461 aatgatgaag cacatgatag aaagtgagtg acgatatgtt aacaacagaa gattttgatt
    11521 ttgacttgcc ggaagagttg atcgcacaaa caccattaaa agaccgcgat cattctcgtt
    11581 tacttgtttt ggatcggaaa attggagaga tggaagataa acatttccat gaaatcattg
    11641 aagagttaca gcctggtgat gcgctagtta tgaataatac acgagttctg cctgctcgtt
    11701 tatatggaga aaaaccagat actggtgcgc atttagaagt ccttcttcta acgaatacac
    11761 aaggagatac gtgggagaca ttgataaaac ctgcgaaacg tgccaaagtt gggacaagaa
    11821 tctcttttgg tgatggcaga ttgcaagcgg tagtgaaaga agaattggaa cacggcggac
    11881 ggatcattga ctttgagtat gaaggaattt ttctagagat tcttgaatca ttgggagaaa
    11941 tgccattgcc tccatatatc aaagaacgtc tggaagaccc agatcgctat caaactgtct
    12001 atgcgaaaga aaatggttct gctgcagcgc caacagctgg attgcatttt acgaaagaat
    12061 tacttgatga gatcgaagca aagggtgttg aattagtcta tctgacacta catgtagggt
    12121 taggtacatt ccgacctgtc agcgtagata aaattgaaga acaccatatg catagtgaat
    12181 tctaccgttt gacggaagaa gcagctgagc gattgaacca agtacgtaaa aatggcggga
    12241 aaatcgtggc tgtcggaaca acttcaattc gaacattaga aacgattgga acgaaattta
    12301 atggagaaat caaagcagac agcggatgga ctgatatttt cattacgcct ggctatgaat
    12361 tcaaagtggt agaagctttc tcaactaatt tccatttgcc aaaatcgact ttagtcatgt
    12421 tagttagtgc ctttgcagga agagaattga ctttatctgc ttaccagcat gcgattgaag
    12481 agcgctatcg tttctttagt tttggagatg cgatgttcgt gaaataaggt tatgaaaagc
    12541 cgctagtttt gaataacaaa aaagctttac aaagatttta tgacaaagaa aaagagctct
    12601 atactttttg atgttggtgg atgacaccag catctttttt attttccaaa acatatagaa
    12661 aagacacctt gaagtccttt agaattaatt tggcgaacca acccaaaagg aggaatcaag
    12721 atgtcttaca ctcaccttat caaagaaaca ttagatattc tagacttatc agttactttt
    12781 aacgaaaatt gtctcacaaa agaaaaatac aaagggcaaa tctgtcatat ttatcgtgga
    12841 aacttgattt atacagccca agaatgcatc cactgtaaac accagattgc ttcagatatt
    12901 gtgcgttggg gtacaacaac cgtccggttg ttaatgaacg atgtttctga atgccgtacc
    12961 tacctcgaat tgaaaaaagc aacgcttcaa atgcaaagct tgtcagcgga cctttgtcgc
    13021 agatacttct gttgcgaaaa aacactgttt catcagtgag aaagtccgct ggtctgtcgt
    13081 gactcgtctg aaaaaaaata cctcaatgac agagatcgcc gctcagaaaa atctatcggt
    13141 ctcttctgtt taccgtatta tgaagcgatt ttatcgtccg ttgaatcctt ttcggacacc
    13201 gttacctaag gtactttgtt ttgatgaatt caaatcagtt cggggtgttt caggtgctat
    13261 gagtttcatc atgatggatg gacaaacaca gcgctt
//
LOCUS       DAFEPQ010000073        13105 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.075, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000073
VERSION     DAFEPQ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.075"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(136..1716)
                     /locus_tag="LP543_002238"
     CDS             complement(136..1716)
                     /locus_tag="LP543_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBL2322386.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2117..3493)
                     /locus_tag="LP543_002239"
     CDS             complement(2117..3493)
                     /locus_tag="LP543_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBL2322387.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3621..4739)
                     /locus_tag="LP543_002240"
     CDS             complement(3621..4739)
                     /locus_tag="LP543_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL2322388.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4881..5315
                     /locus_tag="LP543_002241"
     CDS             4881..5315
                     /locus_tag="LP543_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2322389.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5393..5926)
                     /locus_tag="LP543_002242"
     CDS             complement(5393..5926)
                     /locus_tag="LP543_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBL2322390.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6020..7198)
                     /gene="mutY"
                     /locus_tag="LP543_002243"
     CDS             complement(6020..7198)
                     /gene="mutY"
                     /locus_tag="LP543_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBL2322391.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7191..7988)
                     /gene="recX"
                     /locus_tag="LP543_002244"
     CDS             complement(7191..7988)
                     /gene="recX"
                     /locus_tag="LP543_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBL2322392.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8095..9477
                     /gene="rlmD"
                     /locus_tag="LP543_002245"
     CDS             8095..9477
                     /gene="rlmD"
                     /locus_tag="LP543_002245"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBL2322393.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9651..10229)
                     /locus_tag="LP543_002246"
     CDS             complement(9651..10229)
                     /locus_tag="LP543_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HBL2322394.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10551..11021
                     /locus_tag="LP543_002247"
     CDS             10551..11021
                     /locus_tag="LP543_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBL2322395.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11129..12355)
                     /locus_tag="LP543_002248"
     CDS             complement(11129..12355)
                     /locus_tag="LP543_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL2322396.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         3894 a         2642 c         2199 g         4370 t
ORIGIN      
        1 gacatttcta tgattttaat atagtaaaaa gagccgtcac ggaaaatccg tcacggctct
       61 aaacaaaata tgagaggctg tttttgtttg gctattctta ttacttgatg cagggcagct
      121 tgtgtcaaga cttctttaca tcaaactttt taattttaca ttgggatatt tatccgcaaa
      181 ccagcgttct gcaaattggt tctcgaataa gaacaacggt tggtcgaaac ggtcacgagc
      241 caagatattg cgacttgaac tcatcttctc atccaaatct tctggatcga tccagcgagc
      301 gattttgtgt cccattggag acatgattac ttcggcgtta tactcattta acatacgata
      361 ttggaatact tcgaattgta gctgaccaac agctccaatg atatattctt ccgttagata
      421 tgttttataa agctgaatcg ctccttcttg aactaattga tagattcctt tgtggaagga
      481 tttttgtttc atgacatttt ttgcagtcac tttcataaac agctctggag taaatgaagg
      541 gagttcttca taagcaactt ttagttttcc ttcgaacaat gtatccccaa tctgataatt
      601 gcctgtatca tatacaccga tgatatcgcc tgctactgct ttttcgacgt tttctctcga
      661 atctgccata aattgtgtga cattgcttaa tttcagtttt ttatttgtac gttccaacca
      721 gacatccatt cctcgttcga atgttccaga acagattcgg acaaaagcga ttcggtcacg
      781 atgggctgga ttcatatttg cttggatctt gaatacgaaa cctgaaaatt cttcctcata
      841 aggacttacc atctgatcat cttgtgtttt gtgtccataa ggagctggtg ccaattcaac
      901 gaaagtctct aaaaaagtct gtacaccgaa gttagtcaat gcagaaccaa aaaatactgg
      961 tgtctgatcg ccacgagcaa ttttttcttg attaaatgca tctccagcct ctttgattag
     1021 ttctacttct tctaagactt gttcatataa ggaattttca gttagaggat gattttcggg
     1081 aagcagtcca tcttcatcca attctactaa tcgttcatcc tgataatttt ccggacgata
     1141 aaactctaca cgattatggt agatatcata aagaccttct agccctttac ccataccgat
     1201 tggccaattc atcggataag attcgatatc taatagctct tctaattctt ctaacaattc
     1261 caatggttca cgaccatcac gatctagttt gttgataaaa gtaaatatcg ggatgccacg
     1321 tttttttaca acttggaata atttctttgt ctgtgcttcg atcccttttg cactatcgat
     1381 taccatgacc gcactatcca cggccatcaa agtacgataa gtatcttcag aaaaatcttc
     1441 atgtcctggt gtatccaaga tatttactcg tttcccttga taatcaaact gcataacaga
     1501 actagtaaca gaaatcccac gttgtttctc gatttccatc cagtctgatt tggcaaaatt
     1561 acctgttttt ttgcctttta cggtcccggc ttgacggata gccccaccaa acaatagtaa
     1621 ttgttctgta atcgttgttt tcccagcatc cggatgggaa atgatcgcaa aagttctgcg
     1681 attgtccact tgttgcttta attttggatt attcataacc taactccatt tcttccattc
     1741 aattatttat attttctcta ggcacagatt gtccgatctc ttaaagaaag acaatttcat
     1801 atagggctgt gacaaaagcc gtgtagcagt ctctttttcc gagtaaacgg cgttctagag
     1861 acagcctatc agcaataagc ccacccaagc caaaaatacg aaaagctgtt tttgcttggc
     1921 tgctcttatt acttaatgca ggatagcttt ttcacaacct ttgtcaacta ctacaactga
     1981 atcagtatag catggattca gcgaatgaaa aagccaccaa accataagag tcaaatattt
     2041 ttttgtcagc tgactaaaag aaaagacgtg ttgcttgttt attcaagcaa cacgtctttc
     2101 ataaccaagt ttccaattat cttcttggct cgtcatcttc gaattctttt ctgaagtatc
     2161 ttcgttcttc ttcaggttcc gcttctgttt cttccggatc atgaaaaatg acacggattt
     2221 taaggattcg agaaccttcc atttcttcag aaaccagcgt aatattatcg acatcaaacg
     2281 ataacttttc gccttcatcc ggaatcatgc ctaatgctgt aatcaaataa ccagccatcg
     2341 tatctacatc actcatatgc agatcactgt caaaagcttc attgaactca tcaatcagca
     2401 tacgtccttg gatgatatat tcgtgttcat cgattttttc ataaagattc tcaacttcat
     2461 ctgattcatc atcgatttct ccgacgattt cttctaaaag gtcttctagt gttgctaacc
     2521 caacgacgcc gccatattca tctaataaaa tagccatctg attctgcgtc tttttcattt
     2581 catataacag atcatcaata aagaccgttt caggaacgaa taaaggctcc tgcatgattt
     2641 tcttgatatc taaattatca aagccgaatt tatgggcagc ttttagtaaa ttctttgtat
     2701 gaaggattcc gactacttta tctttgtctt cgttataaac agggattctt gaatagtttt
     2761 cggataaaac ttcattcaca ttttcttcca cagtgtcgtt gatatcaatc ataaatgcat
     2821 ccgttcgtgg gaccatgact tctcgggcaa ctttcgtatc aagagaaaaa accccttgga
     2881 gcatttctaa ttcttcatta ttcaatacgc cttcgttttc aagcatataa cgcatttcat
     2941 ctctggtcat ttttgaatct tcatcatcaa aagtcatcgg cgtgatacgc gccaataaac
     3001 ttgtggaagc agaaagcagc cagacgaagg gcttagctac tacaccaatc aagcgtatca
     3061 gccctgaagt gaactttgcg acttcttctg atttatttaa agcaatacgt ttagggtaca
     3121 attctccaaa aacaatcgat acataggtca aaatagccag cacaatgata tttgcaatat
     3181 tttttgccgc tgtgccgcca cctaataccg gagctaaccg ttccgataaa gtattagcta
     3241 gtgaagcacc agacaaaatg ttgaccaaag tgattcctac ttggattgtc gataggaaat
     3301 tatttggatt ctgcaatatt tttaaaagct tttgagattt cacatctcct tcatcagctt
     3361 tttgctcgat tcgattctta ttgatcgaaa caacagcaat ttcggaagct gctaaaaaag
     3421 cattgatcaa tgtcaaaacg atcaataata aaatctgcgc taacagcgac tgactctcag
     3481 ggtcagcatt cataatcgca ttctctcctt ataaaattaa tttttagttt acgatagaaa
     3541 aaaacgtggc actatagcca cgttaaaatg gtatcacaaa gcaattttct ttgcaataaa
     3601 aacaagtgta ctaaaactac ctacgaggtc gtagcaattt tcttcttgtt ttcattttgc
     3661 ttgtcttctt tgacgatttt gattatatag gaaatgatcg tcttgcagat tgcgtaaaaa
     3721 ggaacaccta agaagatacc aagcaatccg gcaatatttc cagctactag aagaacgata
     3781 atgatcgtta atggatggat tttcagtgat ttcccaatta cattaggata aatgatattc
     3841 ccatccaact gttggacgat tagcacaacc acacaacaaa gtaatgcttt taccggatca
     3901 ttgaagactg ttactagaaa tgcaggtgct agtcctagat aaggtcctaa atatggaatt
     3961 aggtttgtca atcccgcaat gaccccaaat aagaaagcat aatccacacc aattaccata
     4021 tatcctaaaa atgtgaaggt accaacgaat aaacattcga ttgcttgtcc gctaatgtaa
     4081 ttagccaatg ttttattcag ttgccccaat aactccacaa tttggtttct tcttttttct
     4141 gggaaaaact gttggatgtt tggcactagt ttttctccgt ctttcaacat atagaacaag
     4201 atgaatggtg ctgtaatgat tactacggtt gccgaagcga tcgttccaac gatcgaacct
     4261 aaactattgg atactccact aaggaattgc tgaatgattg tcccgtaaga aatgtctaat
     4321 tgctcaaaat atttattgat atctacttcc ttgaacaaag gcaattcggc tacctctttt
     4381 aaccagcttt ctacctgttt gatgaaatca ggcatattag atgccaatga ggaaatttgc
     4441 tgtactagac ttgggatgac actaagtaaa atccaaataa ttgctgcaac taaaaggagc
     4501 agcacaattg cgacagccca tattcttttg atttttgctt tcatcaacag cttgacaata
     4561 ggattcaata aatagtaaag aaagcctgca atcagtacag gtgcaaataa cgtggaaaaa
     4621 aatgtcccga tcggttcgaa aataaagttt atttctgatg aaatcatgat caatgcagct
     4681 atgatcaata attcaagcga ccaaaacatt agtttagact gtctaagttt ttcaaacaat
     4741 agatttcctc ctttctttct tactagtata tattatattc gttaaaaatt cttttattct
     4801 ttttttattt ttgaaaactt gccatacttt ttatttgttt tattttccag tatactatac
     4861 gaaaggaagg tggctagacc atgaaagttg tacaaacaaa agatacaatg agcaatattt
     4921 acttagatgc tgtacggatc aggcatcaag tttttgtagt cgaacagggt gtgcctttgt
     4981 ctcgagaaat agacaaagat gaagcccatt gtatccactt cgtcctgtac tcagataaaa
     5041 aagaaccgca aggaactgta cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc
     5101 ggatggcgat tctttctgaa tacagacacc aagggttagg aaaaatattg attgaagaag
     5161 cagaaaactt tgctaagaat caaggctata atacgatttt attaggggca cagtccacag
     5221 ctgaaacttt ttacgaaaaa ctaggatata cagcttacgg cgatcctttt gaagatgctg
     5281 gcatgcctca tatttatatg aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa
     5341 acagcaagct tttgagaaaa atctctcact ggcttactgt tcttttttgt gtttacttac
     5401 gcgacaattg cttgtaacgg ttataccaaa tatcaatata gccttccgag aacgggccat
     5461 ctccattatt gatccagtca accagaatct tcacattttc ttttaggatg aagtcaatat
     5521 cgttcgggta attcatcatt ttgctgtgca tctcatattc atcgacatcc aatagtcgtt
     5581 tctcgccgtc aggaaatacc ttgatatcca agtcatagtc gatatatttc aaggcctcat
     5641 cgtccagcaa aaaaggtgaa gctagattac aataatagga aacccccttt tctctgatca
     5701 ttgctattac attgaaccag tattttttat gaaaataaac aatcgctggt tcgcgcgtta
     5761 cccatcggcg tccatcagat tcagtgacta atgtgtgatc attcacgccg atcatagagt
     5821 gttcgcttgt ttttaatacc atggtatctc gccacgttcg atgcaatttg ccgtcgtgct
     5881 tgtaactttg gatcgtcaca aactctcctt cttttggaac tcccatagca acccttcctt
     5941 aagcagcatt ttgtgctttg tgtttgggtg ttcattacac cgacatcata attattatat
     6001 caataaaaat gagctttgcc tagaaaggat tcctattgtt tcgatttttc tctaactgat
     6061 ctaccagttt catttggacc ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc
     6121 acttcgaagt tttgttctcg gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa
     6181 aaaggagaac atgccatttc agatgactaa aaatatgagt aacttctcct aaatgtcgtg
     6241 tctgccaaac aaagagttcc tgcttttcaa atgggagatt ttgctcttct tctgctacta
     6301 ggtcatcaaa cagatcgatt tctggctttg tttcccattc ttttttcaat cgctcatact
     6361 cctcttgtgt cacttccatc attggaaatg tccacatatt cgccagcaat ttttggctat
     6421 ctctttcttc aaaataatac gctccagaat gattttgaag agctgcactg atataataaa
     6481 cgtcttttgg ctttgctttt tttgttttta caggaaaact cgtttggatt cctcgtttgt
     6541 tagctaaaca aaaggcttgg atcggacaag tctcacactt tggagaagtt ggtgtacaga
     6601 tagctgaacc tagatccatc attgcttggt tgaattcacc tggatgcttt tcatcaatga
     6661 ttttccgcat cgcttcatca aagattttcc tgctagaagc ttttgcgatg tctgcttcaa
     6721 tgcaaaacaa tctgctgact actcgcatca cgttgccgtc aactgcaggt tctggaagtc
     6781 caaatgcgat acttgcaatc gcgcctgtcg tatatggtcc tattcccttc aatgagctaa
     6841 tttcttcagg cgtttgcggc atttccccat caaattctga catgatctgt ttagcagcag
     6901 cttggatgtt ccgggctctt gagtaataac caaggccctc ccatgctttt aaaagctttt
     6961 cttctggagc atttgctagc tcttcaattg taggaaacca ttccatgaaa cgataaaaat
     7021 aatcgatcac tgtgtctact ctggtttgct gaagcatgat ctcagagatc cagatacgat
     7081 aaggatcacg attatagcgc caaggcaggt ttcttttttc ttgttcatac cattgaatga
     7141 actgatcttg aaattctttt gtttctttat ctgtccattt ttcccattct ttattcactt
     7201 tcctcttcct tttccgtaat gaatcgctgg atcatatcca aatcaaatcc tttttgatat
     7261 aagctttgct ttacttttag atttcttttt ttaggttcaa accttaaatt tcttcgccat
     7321 agtttatctc cttgtttgac taaataatca tattctattt cttgatctgc ttcttgcgga
     7381 agatcagcta tcgcttgttg gatcacttct tgagaaaagc ctttcgtcat caatgtttgt
     7441 tggatttttc tcaataattc tttttggctt tttccgtgat tttttccaaa cgctttttcg
     7501 gctgtctgat gtgcattttg aacttggtct tcaaatgtat attggctgag cgcttgttca
     7561 atgatttcag gtttcaaacc tttttgctgc atctgctgcg ctaatttctt tggtcctttg
     7621 tcacttaagc gcatattcgt gcgcacataa ctttccccat agaccaaatc atcgacaagg
     7681 tccaattctt ttaagcgggc tacgatttta tttcgatctt cttgaaggat ttccttttct
     7741 tttaagtatg ttcggatttc tttttccgat cgcaactgat aacttaagta attcattgct
     7801 aggtgaaaac cgaaatcagc tccgctactc ttcttgatct cttggatagt ttcttcgtct
     7861 aattcccgtc cttttaataa tcgatagcgt actaatacat cttcagatac ttgtaaagct
     7921 tctccttcag ataaatctac gcgatagaaa gggcctttgc ctttactgat tctcgtaacg
     7981 gttaacataa acatcctcct cgaacatata ttctctatca taccacagaa aatccccgta
     8041 atcaggtatt atatgttaga ataaaccaca gaaaaaagaa aaagggagat acttatggca
     8101 gaaacaattg tcaaaactgg acaaacggtt cctttgaaaa tcaagcgttt aggcataaat
     8161 ggtgaaggaa tcggttattt caaacggttg atcatatttg ttccatatgc actaccaaaa
     8221 gaagaagtac tagtgaaaat cacgaaagca actcctcgct atgcagaagg agaattaata
     8281 aaagtcaaaa aaacaagcaa agatcgcgta gtggcaccat gtcctgttta ctatgaatgc
     8341 ggtggctgtc aattacaaca cttagcttat caagcgcaat tggattttaa aaaggatctg
     8401 cttttgcaag cattggaaaa attcaaacca gcaggctttc gatcttatca acttttgccg
     8461 acgatcggga tggatgagcc ttggcattat agaaacaaag cacaatttca gttacgaaaa
     8521 aataagcaga ctcaaaaaat tgaagctggt ttatacgaag caaactctca tcagttagtc
     8581 cctataacag actgcttggt tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt
     8641 caactattga ataaatttga tgcacccatc tatgatgaac ggacaaacag tggaatcttc
     8701 cgcacaatca tggttagaat cggtgttaaa actggggagt tacaagttgt ttttattact
     8761 cgcagtaaaa aattcccgca aaaaaacgca atgatccgtg agatcaatga acgactgccc
     8821 gaagttgttt caatcatgca aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat
     8881 gaaacgatcc acttatgggg aaaagaagcc attgaagaac aaatcaatga agtgactttc
     8941 gatctttctc ctcgtgcctt ctttcaactc aatcctcagc agaccgaatt gctttatggc
     9001 gaaggaatca aagcattaga tgtccagcct aatgaaacga tcgtggatgc ttattgcgga
     9061 gttgggacga tcggcttaag tgtagcaaaa caagccaaag aagtacgggg aatggatacc
     9121 gttcctcaag caatcgctga tgcaaaaaaa aatgcggaaa gattaggata tcacaacact
     9181 cgatatgaag tcgggacagc agagcaagtt ctgcctaaat ggttgaatga aggttttcag
     9241 cctgatggta tcatcgtaga tccaccacgt acgggtttgg ataacgcatt gatccaatca
     9301 ttattgaaat accctccaaa aaaacttgtt tatatctcct gtaatgtttc tacacttgcc
     9361 agagatctgg ttaatttaag taaaaagttc aagattgaat atttacagtc cgtcgacatg
     9421 tttcctcaga ctgcacgttg tgaagttgtc gtgaagatga cgagactcgc tgactaaatg
     9481 aaagaggttg tgaaaaagtc tgccctgtat caagtaataa gaacagccaa acaaaaatag
     9541 ctcttcatgt tttatgaata ctgtacttgt gaccgaagag ttaacaggag gacactattt
     9601 atttaaaaaa agggttgtga catagttttt gtcacagccc tttttgatta ttattggata
     9661 ttcacgtcta tcacttgata aaatgcattt gtcgtatcgc tgatattcca tactgctaaa
     9721 atcacattat atccttttcg atcctgcgga agtgtgatcg tttggttgac tgtttttggt
     9781 ggaacagttg ctttgtcgtc tatttttgtg atcaattcaa aatcactaaa tttcaaaggt
     9841 tgattaggat tccaagttgg tttggtaatg tagtaatccc aagtacttgt ccgatgttgt
     9901 gctgttaaat tccaagtaat atttaatgtt cctggattaa ccatgttttt atgccatctg
     9961 ttagatgtct gttcatctaa aggctcaaag ccgctgactc ctgcactagc aatttttcca
    10021 tcaatgaatg tatttttggt agcttcgata ctttgcggtt cccattgtgc tcttccgaca
    10081 ttttgattta agtttcctgc tgctgatgtt cctaggtatg cacggcttcc tggagaagta
    10141 atataaccat gcgcaaaacc tggaatgcta aaaccgaaca accctaaact aactaaccca
    10201 ataaacaata acgtactaat ctttttcatg tagaaacctc cttcatttga taaccacatt
    10261 gtaaacgctt tattgtatta acacaaacat tatgctttta taattttatg ttgaagtaaa
    10321 aaaggtgaaa aaagtaagat gcagattata acagaagtag acgagaaaag tacaaataga
    10381 aaacatgaga atcaaatcat atgatctttt tcaaaaacat cattcatata aacaaatgtt
    10441 caacttttta ataacaacaa ttatcaattt ttttcttact tcacaaacta aatacaggtc
    10501 ttgctgtgag ccttcatggt aaactactat tatagaaagg agttagtaaa atgaagatga
    10561 taaaagatat tcgtttttgg gtttgtgtta taggaattgt catattaggc tttttatcag
    10621 ggttgcttag cggaaatccc ggagaatatt actattcttt gcaacttcca ccatttgcac
    10681 cacctagctg gatatttggg ccgatgtgga ccttactgta tatattgatg ggtatttctc
    10741 tttatttgtt acttaatcac aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg
    10801 tgattcagtt catttttaat tttatatggt ctgctctatt ttttaattta agaaatattt
    10861 tcattgcagc aatcgatatt acacttttag ttattttctt gtcagtgctt atgtatcagt
    10921 tgtggctaca tcatcgcctg gcaatgtggc tcatgattcc ttattattta tgggtactat
    10981 tcgcaactct attgaattat tcaatttatt ttttaaacta agcgctaaaa agagacgtcc
    11041 tgtaatttaa cacatagaaa aagtgccacc taagttagat tttttgtcta atttaggtgg
    11101 cactctttat taaccaaact atttttttct agtacccact tgttgttcca tactcattat
    11161 tctggtcata accataacca ttttgttcct gtccagcgta aggatcataa ctttgagtat
    11221 cttgacttgg tgtatagtac tgacttgtat cttctgtttg ctgataatag gtattcggat
    11281 cttggtactg atcatatgtg ttttctgaat aggaagaata atcggtgtac ccttcactat
    11341 tcgatccatc accgtttgtc gaatcgccat cttccgaatc atcaaagaac atcattgaag
    11401 cattctcatt tttcaaatta ggtaatgtat cgcttgtttt tatatttaat tgtttcttca
    11461 gttcattttg gatcgaaaga agatcatttt ttcctaaaat ttgatagtag atatcattga
    11521 tcatttgtcc ttcgccttgc aactggtctt gtttgattgt ttcaaacgca ggggtgtagt
    11581 tcgtcgctac atcaagcatg tcatcccaat ctaagtccgt cgtcacattg ttctcaactg
    11641 cttttaaaat ttttctataa ttgcttacgc catctaaact taaaacttta ttgacgattt
    11701 tcgtcactac ttcccgctga cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc
    11761 gcattcgaga ataggctaaa cctttttctc cattcaatgt ttgttcacct actggtacat
    11821 ggacaccatc caaagtaaag tcaattttat tattcacttt gatgccaccg accgcattga
    11881 tcaaatcttc catgccatcc aaattgatcg ttacataatg atcaatagga atatcgagca
    11941 gttgctcaat tgaatccatg gccatttcga caccaccaaa tgcataggca tgatttaatt
    12001 tatctaccgt tccataacct acgatattcg tataaatatc tcggtccaaa ctgatgatcg
    12061 ttgatttttt ttgtttcgga ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt
    12121 ccgttcttcc taatccaccg gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt
    12181 catcaatgct tactctctca gaacgtttgg aggatattcg attagagttt ttagaaattt
    12241 tgtttactgt ttggctcgct tctcccataa atctgattcc gtatgctgat aatccggcaa
    12301 tcagcaaaac aaatacccct aacaaggtta atagaacttt ttgccatcgc ttcataatac
    12361 acctctgttt cacattttct aaaatattgt ctcatataac tgttcaatca gcaaattcac
    12421 tttttaatca taagaaaact ttaaaaaaca tttttgtgta acgattacta ttttttaaaa
    12481 tttctcaaaa ttcttaacta tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac
    12541 gcagttcgca atcgttttat ctttttttac gtattttttc cagaaaaagc gaacaacaag
    12601 gtattttcca aataatcgtg cagtaaaatg atcgcacata cctttcccat ctttttaaca
    12661 gtctttgcct accgttcaga ttatcattcg atttcacgaa aatttcttcc tatgactggt
    12721 ccttttgtta aacccatctt ctattcgttt tatttccagc tcactgcatg gctcttaaaa
    12781 tgactcgaac tagtgatttc attaccatca tcttttaatg gatagatatg ctcctcttca
    12841 aagtaaaacg attttctttt aaagtaatag aattgacatg gtaatgagta tgtatactag
    12901 tggtttcaca aatggtcttt tattcaacat ccaaaaactg aaatttcttt aaaatccatt
    12961 actaacgaag aaaatgttcc ttgcttgata attagttagg acatgtcaag tgaacaaatg
    13021 catttactta gccaaaaaat ctaaaaactt cataaataga caaaaagaca cgactctcta
    13081 agaagtcgtg tctttattca acaaa
//
LOCUS       DAFEPQ010000074        11398 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.076, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000074
VERSION     DAFEPQ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.076"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(362..1480)
                     /locus_tag="LP543_002249"
     CDS             complement(362..1480)
                     /locus_tag="LP543_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322397.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1951..3258
                     /locus_tag="LP543_002250"
     CDS             1951..3258
                     /locus_tag="LP543_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBL2322398.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3377..4576
                     /locus_tag="LP543_002251"
     CDS             3377..4576
                     /locus_tag="LP543_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBL2322399.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4603..5871)
                     /locus_tag="LP543_002252"
     CDS             complement(4603..5871)
                     /locus_tag="LP543_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBL2322400.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6034..6680)
                     /locus_tag="LP543_002253"
                     /pseudo
     CDS             complement(6034..6680)
                     /locus_tag="LP543_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7088..7591)
                     /locus_tag="LP543_002254"
     CDS             complement(7088..7591)
                     /locus_tag="LP543_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL2322401.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSPSMRKRWQRY
                     FGKESSI"
     gene            complement(7714..8478)
                     /locus_tag="LP543_002255"
     CDS             complement(7714..8478)
                     /locus_tag="LP543_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBL2322402.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8586..8861
                     /locus_tag="LP543_002256"
     CDS             8586..8861
                     /locus_tag="LP543_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBL2322403.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9299..10249
                     /gene="yidC"
                     /locus_tag="LP543_002257"
     CDS             9299..10249
                     /gene="yidC"
                     /locus_tag="LP543_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBL2322404.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10428..10628)
                     /locus_tag="LP543_002258"
     CDS             complement(10428..10628)
                     /locus_tag="LP543_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322405.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT         3428 a         2392 c         2013 g         3565 t
ORIGIN      
        1 aacggtgttc cagaagtagc ttcttcggaa ataagccgaa atttcacaaa aatttgagaa
       61 ataatttttg tcaatctctt cttatttctt gaagctgacc acttctgtca caacctcttc
      121 cttaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaccaaaca aaaaatatga
      181 agagctattt ttgcttgctg ttcttattac ttgatgcaga acagcttttt cacaacctct
      241 catacacggt gtttaatcag ctgaccttcg gtattagctc aaaaaacgga aaaatatgtg
      301 aaactatttt tccgtttgtt tctctaatac tcaggtcaaa acactgattt cacaacctct
      361 tttaataacg aagaatcgct gtcagactct tttcatctgg cgcatctttt tgatccaaaa
      421 taaacacttt cccgccatta atgatcacat catctgctaa tgcattcaac acttgacgcc
      481 gatcatattc tgtttcagga tcttcaccga aaccatcagc taagtttgac gtagcgataa
      541 ataaatgtga cacttttcct tctttcgcag ctggaacgat atcaactaac tgatcgatga
      601 atttcttatc taagagttta ttgtattctt ttgattcttg agctgcgagt tcacttgaca
      661 gcttcttcgc cccttcttcg atatctctaa aggatagctg cgcaggagaa gcagaaatcg
      721 caacgtctgc acaataatag ggatttttag caattttttt gaacatcgtc tgattttctg
      781 gtaatgcaaa taaataaatc ggccattgtt caggattttc gaaagaatcc ttcaagtacg
      841 tatcaacagc ttggtaatag ttgacccaat cgatctctac ttcttcatct tttggattta
      901 cgccatggaa agttactcca tctcgggctc cgcctcctcc attagaagag aagttcaaac
      961 cgccacctgt cagttcagat cccaatgcag taacgacatc aaccggtgcc ccttctggta
     1021 gagatacttc ttttacttgt ttgttgctga cctcatacaa tttcatggag tcgcggttca
     1081 atcccaataa ataataagaa tagttgaact gcccattttt gataatagcc agtaaataag
     1141 gaagatcact tacatagtat tgatcgtcta ccgggatact caaacgatgg atataggtct
     1201 ggtcagcagt tataatgacc gctacactcg tagttgcact acgccaaaac gaaggatctc
     1261 ctaacaaatt ttcaattttt tcttggaacg gggaccagtc atgttcggaa tattttttct
     1321 caaaacgttt ctttgcttct ttcgcaaaat ttttgaattt gatctgatct ttttccacat
     1381 cttgatgggc gacatgcgta ttcagcatga tcgtgacgaa tggtccttta gcgccttctg
     1441 aagtcaattg tgtcaataat tctttatcct tttttgccat aaacgtaccc ctcctaaaaa
     1501 aatcattcct ttgtttctat ctaaagtcta ccacaacact atttttccat acaataaaac
     1561 gtatctaaaa aaataaaacg gtttctacgg ttttaaattt agctattttt ttaagttatc
     1621 ttcataattt cctcaattat ttagccccag tcttcaacta ttcttcatga aatgctgata
     1681 aactaagaaa aagaataaaa gaggtggatc ttatgcattt gattatgatt ttcagtttat
     1741 taagtattat cagtttattt gctttcagcg cttcccgctt attagatgcg gaaacacagc
     1801 ctattcctgt aaaagtacgt aaaaacgacc gctaaacttc cataacgcgc gttcttatcc
     1861 taaagttttt tcagaaacat tgaaactctc tcccctctct gataaaattt taatttgtag
     1921 cgaaacaaat taattacaag gagatcggat atgtccataa aaaaaacacg tctagtccta
     1981 ttattggcta gtctcattct ttgccttgct tcttttctac cagctgttgc tgtgcaagct
     2041 gaagatacgt tcaaagtaaa tgcaaaagca gcttttgctg tagatgcgca aagcggtaag
     2101 attctttatg atcaagatgg agaaaaaacc atggggatcg cttcaatcac aaaaatcatc
     2161 ggtctttaca tcgtccttga tcaagtaaaa gaaggaaaac tgtcatgggg tgataaagtt
     2221 tcgatttcag attatgcaga aaatttgagt atcaccccag atttatcgaa tgttcctttg
     2281 cataaagaaa acacctacac tgtcaaagaa ttattcgact ctgcaatcat ccagtcagcc
     2341 aatgcttcaa tggtggcctt ggcagaaaaa atctctggca gcgaagctaa attcactgaa
     2401 cgaatgaaag aacaattgaa agattgggga atcaaagatg caacaatcgt taatgcatca
     2461 ggattgaaca actcttatct tggcgaaaac cgaccagaag gcactggaga aaacgacgaa
     2521 aatcaaatgt ctgctcaaga tgtggccatc gttgcccgcc atttgatctt ggatttccca
     2581 gaaatactgg atgtatcaag tactacgacc caaatgtttg gcgaaaatac gcaatcacca
     2641 gtggaaatgg tcaactggaa ctggatgctc ccaggattcg ttaattacaa ggaaggcgtg
     2701 gacggattaa aaactggtac gactgagctt gcaggtgctt gtttcgttgg tacgatcact
     2761 aaaaacgatc aacggatcat cactgtcgta ttgaatgcgg acggccatgc ggaaaacccg
     2821 agtgcccgct tcaatgaaac agaccgtttg atggactact gctatgacaa ttggtcagaa
     2881 aaagagctcg gaaaagctaa cgcaagtatt cctagtctaa aaaccattga tgtaaaagac
     2941 ggaaaagaat caacagttcc tgttgtgctt aaaagccctg taaaagtatg ggtacgaaac
     3001 gatatggata ctggcaaatt aacgatcacg ccaacacttg acaagaagca agtcactgac
     3061 aatgagatcc aagcacctat ggataaaggt acaaaaatcg gtaccgctac gatcaccttg
     3121 gcagacgata aattaggcta tttagaagac aacaatgcac catcaacaga gatcattacg
     3181 gataaaacag tggaaaaagc gaatatattc gtgattggct ggcgacgcgt cgcagaattt
     3241 ttcggcaact tgttttaatt gaacaataag aaaaacatct ttccatcgga aagatgtttt
     3301 tttgcttttt ctagtttaaa taacagcttt tttcatctat tttcgttata ctgtataaga
     3361 aaaaggagtt gaatttatga atctaaccag agatctgatc tttttagttc tctatcttat
     3421 tggggtattc tctatcgttt attggcgaaa acagaagaag gatccttcct tgttctttta
     3481 cggcagtacg tggacattta cggtaatctt aacttatttt gtcgtgttgg aaatttttta
     3541 ttcgaccttt tattttttga ttccattgtt gttctttagt atctttgctt tttcttattt
     3601 cacagaaaaa cgaaggctac tgaatggtct tttattcaat gttttactca tcagtttcgg
     3661 catctacttg tttgtcttgc tgtatgaaac gcaaaatatt tttcttggcg gactgatcgc
     3721 cttgatcacg attccattgc tacttgtcct tttatttggt atttatggat tgattgtttt
     3781 tcttttttgg aacggggtca cagttttacg tagagaatcg cattcattag ctaatctttt
     3841 aacgttgata ctagccattt ttcttacact gtttcttgtt tttgactttt tcttgttaaa
     3901 atatttgccg caatggataa atgctttgtt tttctgcgtt cccctgatct tgatctatct
     3961 atttatcgta ttctacaact ttttgactgt ctcattcttg tatcagttca accggcctcg
     4021 atacaatcaa gacttcatcg ttgtgttagg cgctggattg atcaatggag aaacagtttc
     4081 tcctcttcta gccaagcgaa tcaataaggc aattgctttt tatcgggctc aaagccgtgc
     4141 tacattaaat ccgcccatct tattgatgtc tggcgggcaa ggagcagacg aaaaagtacc
     4201 agaggccatt gcgatgaaac aatatgccat ggaacaagga atccctgaaa gagacattct
     4261 tgtcgaaacc aattcgacaa ccactttgga aaacatgctc tattcaaagg aaatcatgga
     4321 tcagcaaatg aaagggccat accgcgcgat ctttagttcc aacaattacc acatttttag
     4381 agccggtctt tatgcgcggc aagccaaatt gaaagcaaat ggtattggtg caaaaacagc
     4441 tttctactac ttgcctaatg cgtttttaag agaatacctc gccatcttga tgatgaacaa
     4501 aaaaagacat atgatcattt gcggattgct tttcgcaggc tccgcattct tatcgctgat
     4561 tactttactt ttctaaaaaa acaaggtgtg acaaaacata tgtcacacct tgtttttttc
     4621 tttcattctc ttaactattg gcaaaaagag tatccctttt ttgaccgcta cttgctgagc
     4681 agaataaata ccgctgttgc ctgaacacat aaagctgatc aagcagatca taaagaagta
     4741 tactgccgct tgtgaaccga acaattcgat ccccatcaca aagcaggcaa tcggtgtatt
     4801 tgtcgcacca gaaaatactc cgataaagcc caatcctgca agaaaaggaa cagaaacatg
     4861 gagcaaaggc gctagtgctg caccaagcgt tgcaccaatt tcaaacaatg gtgtcacttc
     4921 tcccccttga tatcccgcgc ctaaagacaa aactgtgaag aataacttcc caataaaatc
     4981 atagacatgt gcctgtccat taaatgattc ttgaagtaac ggaagactca atccgagata
     5041 acgttgtgtt tgcaaaatca atgctgctgc cacaactaca gctccaccta tcgtatttcg
     5101 acaaacagga tgaggaatcc attgtgcata tcgcgctttt aaaaagacga tcgaacggct
     5161 gaatacccaa ccaatcaaac caaaagcaat cccagcaaca aaaactttga agaacagcaa
     5221 catactccat tcaggaatca cacccatttg gtaatgtaga tgagaaacac catagctttc
     5281 tgtcatgaaa tttgcaaaca gtgcagcaaa gaagctaggg aagatcgctt ctgttcggac
     5341 tttcccgatt gccaacactt ctaatccaaa taatgttcca gctaaaggtg tgccaaatac
     5401 agaactaaaa cccgcactga ttccactgat aatcaagatt tcccgttctg atttatccaa
     5461 tcggaacaaa cgtgccgtat tatctgctaa cgctcccccc atttggactg ctgttccttc
     5521 acggccaaca gatcccccaa ataaatgggt agttatggtt ccaaacagtg taagtggaat
     5581 aagccgcaac gggatttttt cttctccacc atttccttga tcgatcacta agttgtttcc
     5641 acgactagaa agtccgccgt gatacgcata gagataagca aaaagcgcac cactaactgg
     5701 tagtagacaa aggagccaag gatgtgatag tcggatatct gtcacaactg tcaggctatt
     5761 taagaaaaaa gcagacaagc tccccatgaa tataccgaga atcgctgtga tcaccagcca
     5821 cttcagcata tacactaaaa tgcgtactgg actcggtaat tgtttttcca taaaaaattc
     5881 ctcctcaata aaaaaacgcc tactaaagaa aggggctgtc cccagacttt agtaggcgtc
     5941 attagctatc tttcgttagc atttaaaggc gaacaccatc acctattcac ttccattaaa
     6001 agatagcata tttttctgta aaaaacaata aaattatcgt tctttcttta cacgttcagc
     6061 aaattcatag aaaggagcca atttcgttaa ctgttgatta cattggtcac tatcatgaca
     6121 aatataattc ccctttttcg tatacgtgcc atcagaacct gctttcgttg tagaaagaaa
     6181 aagcgaaaca ttcgatgttt tctgacagat tgcacagatt cccttattta tcgttggtga
     6241 taaagtaccg ctaatccctt ttaatttttc atcttcgtaa tagaccatga attttttctg
     6301 cgtacctgga tcattccagc cgatgtacgt atgttcttgc aagtcgattt cattccaagc
     6361 tggttgtttg agttttttca cttttcggaa taatttctcg atttgtggtt tgctaggctg
     6421 cataaacggc tgaacgattt gcttcagctc ctccaatgct ctttctgctt gcgcttttgt
     6481 cagttttacg tctaaaactg tcgttaaaaa tgtatcgatt ctggcagttc gtgatctaat
     6541 atgtctcgaa tcttttcagc tgctagtgcc tgaactgttt gtaccgtttt tggatcattg
     6601 acagatgagt acgcatttaa taactgttct acttctctta ttataaaaaa atattggtat
     6661 ggttttattg tttgttccat gattttaaag tcctttcttc taaccaaagt gcatactttg
     6721 tagaaaaaag tgatcttctg caaagtatgt gttacctgaa gatcccggac tcttttaaat
     6781 aagatacgtt ttgcatctta tcatcacccc ctttaatgct tattattcta tgtacatgat
     6841 tcattcgata aataaaagta aaccgtatct tgaacagata gtcaactgca aacagaaaaa
     6901 aggactgtga caaaagtcgt gtcaaagtcc cttgtttcga atgaatggtg ttcaaaagct
     6961 ggcatctgcg gtaataagcc gaaacttcac aaaaatttga gaagcaattt tcgtaaattc
     7021 cttcttattt cttgaagctg accacttctg tcacaacctc aatatggtct attattggtt
     7081 ttatctatta aatcgaggat tctttcccaa agtaacgttg ccatctctta cgcatacttg
     7141 gtgacatagg ctgagcggct tctttgactg cgcagtattt gtctacgaat gttacgatat
     7201 atccttcttt gtacttaggc ggtgcaatcg ttgcgcccca catgtgcttc aaaatgatat
     7261 ctcgctcaag atccgacaat tcggtgatct tttctgcatt ctttacagca attcttggat
     7321 ggatatacgc atgtgtgcct tcatcaaatt tcgtcgtacg ccaatcatag tagaacagat
     7381 catgcaaaag tccagcacgc gctgttgctt tagcattgcc accccatttt tttgcgagca
     7441 gataactata gtaagaaaca ctgatggaat gctccaaacg agtggaatga acatgctgtg
     7501 tgtaattcgc taattttttc actgcttctg tttctaaaag atcttctaca taagacatat
     7561 attcttcgtc ttcacgccat ttttctgtca taattatcga tctgccacct ttttgaattt
     7621 cattttcttc tgaaattgat ctaagtatac caccagtgtt cggattattc tagtcgcctt
     7681 ggataaaata gccaaagctt aatagttcct taatcataaa gaaaaatgat aaagaacaat
     7741 ccctgccgca accccaacat tcaacgattc cgcatttcct ttcatcggga tatatacgtt
     7801 ctgatcagtc aaagacaaga tttcctgact taccccctga ccttcgttcc ccatgattat
     7861 tgcgaagtta tctgattttt ctactttgtc taatgcgtgt gcttcttcat ttaattctgt
     7921 gccatacact gtaaaccctt tgtctttcat taaaggaatc acttctgtca aatctttttg
     7981 caacacaggc aaatgataat tgcttccctg catgctgcgc agcacctttg tactataaat
     8041 atctgccgtt cctttgccta ataaaacacc agccatatca aatgcatctg ccgtacggat
     8101 catcgtccct acatttccag gatcttgtac attatctaac aacagccatc ctcctgaaaa
     8161 atctggcgtg ccttcagccg ttaacgccat cactgctaag attccttgtg gtgtaggtag
     8221 atccgataca gcatccatga cctcatccga aactagaaaa gtagaaatct ctttgtttgc
     8281 ggccagccag tctccccatt ctttcatgcc tcgctgtgtc acaaaaagtt cttttatttc
     8341 tgcatcagac gacgcagctt cttcaatcag atggaaccct tccaaaagat attcttcctt
     8401 ttcttcccga tatttctttt tatgtagttt ttttaattct ttgattcttg gattttttgc
     8461 tgattggatc tctttcacat tcctcacctc cccatgatta tatcatggaa taggtgcttg
     8521 agcttgtttc atcctaaaaa aatcggtatc attaatttat aaaataggaa agaaaggtga
     8581 cgaacatgcg caaagttaaa atgaacgtcc aaggacgcgt ccaaggcgta ggatttcgct
     8641 acatgaccaa gatggtcgct gatcaactag gcgtgactgg aacggtaaaa aacgaagatg
     8701 acggctcagt cacgatcgaa gcaatcggca atgacgacat catccagaaa tttattgaag
     8761 aagtcaagaa atctccgagc ccaagtggac gagtgcagta tgtggatgtc caagaagacc
     8821 cgatgattga ggaacgaaag aaatttgatg tggtgggata aggttgtgaa aaagctggtc
     8881 tgcatcaagc attaaggaca ggttgtgaaa aatagctctc catatttttc aacaggctgg
     8941 acttaatgcc gtagatgcaa gcttttgaac accgtcgata aggttattct tgaggcgctt
     9001 tgcctcaaaa attaaggaca gcagtgaaaa agctgatcta tcacttgcag tgagttcatt
     9061 tgactccgct ttttcgggca gaaaaaaagt ttttagttta ttaaagtaag aaagaaacac
     9121 ttggaatcat tctacttttt cctagtattt ctttcttgat tttttgtctt tttttcaaaa
     9181 tttttcgcta cgtaaaagaa cgaatattta tatgaaattt ctatgaagtt gattgaattc
     9241 catgcgcttt tttagtatag ttaagtttgt gtaaaaatta tagaagagga agtattctat
     9301 gaataaatgg aaaaactggt tattgggatc cggacttgtc agtatcctgc tctttttatc
     9361 tggctgtgtg cggatggatt caaacggaaa ccctgacact tcgggaatcg tttatcgagt
     9421 actcgtccat cctatgggcc aagcgattac atatctagtt gaaaatttca attggtctta
     9481 tggctgggca gttattgcaa tgactgtgat cgtccgtatc atcatcttgc cactaggtat
     9541 tagtcaatcg aaaaaaacaa tgatccaatc tgaaaaaatg caagcgttaa aaccacaagt
     9601 tgaagcagcc caacaaaaat tgaaagctgc aacaacacgt gaagaacaaa tggcagcaca
     9661 agcagaaatg cagcaagtat accgcgaaaa cggattgagt atgaccggcg gaatcggctg
     9721 tctgcctcta ttgatccaaa tgccgatctt ctctgccttg tactttacgg cacgttatac
     9781 agaaggtatc cgggaatcat ctttctacgg catcgattta ggctcaccaa gtatggtact
     9841 tgttgccatt gcaggggtcg cttatctgct tcaaggatac atctcaacga tcggtattcc
     9901 tgaagaacag aaaaagacga tgcgtacgat gctgatcgtc tctcctgcta tgatcgtctt
     9961 catgtcgatc agtgcgccag ccggtgttac actttactgg gtagtcggtg gtgtgttcag
    10021 ctgtctgcaa acattcatca caaacgttat gatgaaacca cgtttgaaag cacaagtggc
    10081 agaagagttg aaacaaaacc caccaaaaca agtcgtcact ccaagaattg atgtgacaga
    10141 atcagctaaa ccagctgaca gcaaacaagc aaccaaacaa atcaagcaac ccaaaaacag
    10201 tacaaacaat aaaggccgca acgcaggaaa gcagcagaag cggaagtaga tagtaaataa
    10261 gagtgtaata aaaattcatt aacagtttta gcaattaaaa atatcgacca agatttttga
    10321 aaaagaaatc tggtcgatat ttttatttaa gtgaatgttt aatttgaatc atcgattgat
    10381 cgctatctaa ttgcagcagc atctttttat gaagaaacaa ctttttctta tctatcaacg
    10441 tagacaggtg ttgctgtttc ttctaatagt gtacttaatt ccttaatatt ttgttttcct
    10501 tcttctaaca tccaactact cgctttttct tcgccttcgg taacaaaagg cgccactccc
    10561 ttcgcccatg ttgctcgccc acataagaca ccattctcca taaaggacaa cgcggagata
    10621 ccacccatct aaataacatc cataaacctt tctagtagaa gaatagtgat agtatccatc
    10681 cctttttcta ctgggaatac aattacacga ttcaaagctt gatacacaac atttcccttc
    10741 ttataaaccg ctattgtaac cgaattgttt tgaatcacct ttttgggata gaaactggct
    10801 cctttccgca acttcttaaa tgttggtttc ttttatactt tctttgtagc aactacaaat
    10861 ttcttcaaat ctctaaatgt ttgcataaca accgcctttc tgacttttac tcaggatggt
    10921 atttaaaaat cagcaaaata aggcttctct ccatcttttt tatttcctag ctgctttaaa
    10981 gacactcttg ctgtttttta atctctatca agggggctct gaatctattt tgatgaactt
    11041 atcttattaa tttctcaaat gagctgtcag acacaactgt attttttcaa tctcataccg
    11101 gacaactatg ccagcttaaa aaaatttcct ctggcaaaac ttctttattg aaaaatgaca
    11161 atgaaaaaag accattcttt ttgttgaatg ctcattgtgt aaacatattt cctgcgactg
    11221 ctcttcgaac cattctcagt gcaatcccta atttatctat taaatcttct tttagttcct
    11281 catctttcat attcaaaatt tcctttgtat gttacacaag tttgttctct aaataacgcg
    11341 aattgtaaaa ttaagttaga aaaataaaaa ggcatttatg gtacactcaa attgtatt
//
LOCUS       DAFEPQ010000075        11338 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.077, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000075
VERSION     DAFEPQ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.077"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            503..682
                     /locus_tag="LP543_002259"
     CDS             503..682
                     /locus_tag="LP543_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322406.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(728..1333)
                     /locus_tag="LP543_002260"
     CDS             complement(728..1333)
                     /locus_tag="LP543_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2322407.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            1515..1850
                     /locus_tag="LP543_002261"
                     /pseudo
     CDS             1515..1850
                     /locus_tag="LP543_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2015..2242
                     /locus_tag="LP543_002262"
                     /pseudo
     CDS             2015..2242
                     /locus_tag="LP543_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2368..3339
                     /locus_tag="LP543_002263"
     CDS             2368..3339
                     /locus_tag="LP543_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2322408.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICKENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            3479..4792
                     /locus_tag="LP543_002264"
     CDS             3479..4792
                     /locus_tag="LP543_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBL2322409.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            4835..5701
                     /locus_tag="LP543_002265"
     CDS             4835..5701
                     /locus_tag="LP543_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2322410.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLMPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            5698..6546
                     /locus_tag="LP543_002266"
     CDS             5698..6546
                     /locus_tag="LP543_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2322411.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            6560..8032
                     /locus_tag="LP543_002267"
     CDS             6560..8032
                     /locus_tag="LP543_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HBL2322412.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8049..8921
                     /locus_tag="LP543_002268"
     CDS             8049..8921
                     /locus_tag="LP543_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL2322413.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PYPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(9157..10539)
                     /locus_tag="LP543_002269"
                     /pseudo
     CDS             complement(9157..10539)
                     /locus_tag="LP543_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11001..11282
                     /locus_tag="LP543_002270"
                     /pseudo
     CDS             11001..11282
                     /locus_tag="LP543_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
BASE COUNT         3497 a         1947 c         2291 g         3603 t
ORIGIN      
        1 aattaaacag aattgatcag aaacaagcga agccttctct cggttcgctt gttttgctat
       61 ttattagttg gctcagggca actctaagct cgtttgaaag aaaaccgatg gataatcccc
      121 ttgtttgtac cactttagaa tacatggatg tacgtattgc cattgactcc tattagttac
      181 tgaaaaaaaa tttaaacaag ttgagtattg cagataaata gctccagtga ggtgaataac
      241 tgctccactc aaggcaatag atagctccat ccagggcaac aaactgctcc actttcatat
      301 ttaatagatg aataaccttc tgtataatag aaaggcatac ctttcggtat gacaacttat
      361 acagaatgtt attttattgg ttcactatcg aaaaattttg gagctttatt tttccggtat
      421 ttctcaacga acgattagtt taactgtggg aagctccaga catacgattc gagaagttac
      481 ttctcgagca gacaggaagg gggtgactga actaaccgct gatatgagcg atacttggct
      541 gcaggaatat ctttttccgg aaaaacaaac aacagcacga ggttaccttc ctgtagactg
      601 ggaatatgtt cataaagaac taatgaaaaa gaatatgaca ttgaaactcc ttcatagaga
      661 atatggggta catggaacat agaaacgaaa atgcacggta gaaaagcgaa tgaatatagt
      721 ataaattcta caaatcattc gctttcttta ttttatatat ggttggtcga gagagaccat
      781 tttctttggc aatttctgca actggaactc cttgatttaa catgtccacc acagttttaa
      841 agactaatcg tttttgcgga tctgttgagt tctcagaata ttcaattggt cgccccttat
      901 atcgaccagc ttttttggcc aataatactc cttgccgctg acgttcacgg attttctcac
      961 gttcgttttg tgcaatataa cttagcaatg ataaaaacat atcgaacata gcagtatcta
     1021 ggagttcatt tccagtatta aaatctaaaa actgtgcatc tagtattttt aatgctactt
     1081 ttttctgttt cataaaggta actgtatttt ttatgtcttc ataatctctg cctaggcgat
     1141 ccagtgatgc aacaatgaca caatcacctt ctcggatata atttagtaat ttttgaaact
     1201 caggtcgctg gatatcttta ccactggctt tttcttggaa taatttatca atgttaaggt
     1261 cttccatcat tgccaattgt cgatctaaat tttgatcttg tgaagacact ctaatatatc
     1321 caatttttgc cataaaaact ccttttaaaa aatgatataa tttacatgta aaatttactt
     1381 gtagttttac tgttatcata gcatttttaa aaataaattt taagtctact tataatttac
     1441 tttggaggtt ttgaatgaat gaaattaagc gcaataaaaa ttcttttggt gtttatttag
     1501 gagagcctag ctcggaacag ttaaataaag gtgaagctcg tcatgctcta tgtcgaaata
     1561 tattttatgg tagaaaaggc aaactttatc aaacttatta cgacggtatg gaggaacagc
     1621 tcaacgcatt aggattagta actaatgcta tcatatattg gaattcacta tatttagaaa
     1681 aagtctttga acaaattaaa acggaagggt tcgactgttc cgaagaagtg ttacgcaagc
     1741 aatttccatt aattagtgat cacattaatt ttgttggcaa atatagtttc cgctatgaaa
     1801 aagaattatc gaatggaata ctacgtccgt taaatataga gaattattga gaatgttcgt
     1861 cttttggttc ctaccgtgca ttttcgcacg gatgttccag atactatttt tgaatgtata
     1921 aaattgaaaa cacaagaata ctaataaatc aacgattctt gtgtttttaa tttttcacta
     1981 ccgtgcattt tcgtttctat gttccatgta ccccaatctt gaaaaattaa ttaaagactt
     2041 agataaagcc attgacgact tagtcgtggt gctaccggag tatcaatgtt taaccagtat
     2101 cccaggtatt ggtcctgttt aggctgctgg aatactcgct gaaattggtc caattgaacg
     2161 tttttctgat gaaataaaat tgacaaagta tgccggccta tattggcgac agagtcaatc
     2221 cggtaaatct gaatacgaaa atttttttac gaaaagcttc acgtctaata tctattttaa
     2281 aataaaaata ttcctgctaa tttggaatcg tttccttctt ttggtataat aatacctgaa
     2341 attcttttga gaggagttgt tcgtgaagtg aaaactacta tgaaggatgt tgctgcgtta
     2401 gcaggagttg gtgtgggtac ggtatctaga gtaataaatg gagtaaaggt gaaagaggtt
     2461 accaaagaaa aagtaatggc tgcaattagt gagcttaatt atgagcctga tgaatatgcc
     2521 agaggtatga aaacgaatag tagcaataca atagctctta ttttacctac gatttggcat
     2581 cccttctttt ccgagtttgc ctactatgta gaggaagctc tcagccaaaa aaattataag
     2641 ctttttttgt gtaatgcgaa cggcagtgcg gaaaaagaaa aagaatatat ccaaatggtc
     2701 aagcagagta aagtagacgg aattataggt attacctatt cagacattga taaatatgta
     2761 tcatcaaatt taccatttgt cagtatagac cgccattttt cggaacaggt ttcttatgta
     2821 acggcagata attatttagg tgggcaaatt gccgctcaag aactacttac tcgtaactgt
     2881 aaaaacttgt gctatgttgg gggtgtttca ccttacccta acgaaacaaa caatcgtaaa
     2941 gctggttttt acaatatttg taaggaaaat aaaatagctg tgaaggtatt ggatatgcct
     3001 gaaccaatta gcaatttaga ggaacaattg ctcgcttttt tttcggataa tcctgaaatt
     3061 gatagcattt ttaccatcaa tgatttcatg gctttggatg tgataaaagc ccaaaaaaaa
     3121 ctagggaagg aagcacctaa ggattttcaa attattggtt ttgatggagt caagctatcg
     3181 ttgcaaaaag actatttact atcaacgatt gtacagccga tagaggaaat ggccaaggtt
     3241 tcagtagaaa cactcatggc gatgatttta aatgaagaac cgcctaagcg tgtggtagta
     3301 ccggttttct ttcacgctgg agagacgact aaagagtaaa aaaccggttt ataacgattt
     3361 ttttgagata ggttgacaga aagtgatttc aatgatatct taaaaccaat taattggaaa
     3421 cgttaccaat taattgaaaa ataaaacgga aacgtttcaa aaaataggag gaacatcgat
     3481 gaaaaagaga atgttagcaa ttgcagcaat tttagcatta acagctggag gacttgcagg
     3541 atgtggcagt aaggagaaaa gttcaggtag cagtgatcca ggagaaatta agatttgggt
     3601 tcagttttct gatgaaacgc ctgaaggaaa agcttgggca gagattgtga acaattttaa
     3661 tgatgaatac gagggtgaat ataaagctgt tactgagtat attcctcgta gcggaagcgg
     3721 cggtggatat gaggataaag taaatgctgc aattacaacc aatacccttc ctgatgttat
     3781 tacgttagat ggaccgaata ctgcagctta tgctcattca ggagtcattg ctccattaga
     3841 tgattatttg aaagatgcgg acatggatga tgtattagac agcattattc agcaaggaac
     3901 ctacgatggc aaaatgtatg cgtttggttt ctcagagtcc agtgtgggtg tctattacaa
     3961 taagtcgctg ttcaaagatg ctggaattac agaagatcaa ttgccgacgt tagaagatcc
     4021 ttggacttgg acggaatttc aagcaatttg ccaaaagctg actgataaat acaaaacgcc
     4081 tgcaattgat atgcaaatgg cgtcgggaga cgaaatgctg acctacgcat atacaccatt
     4141 tatatggtcg aatggcggcg atgtcgttaa tgctgatgga accaaagccg aagactattt
     4201 taacggtaaa aaatccgttg aagcgttgga atttattcaa gaattggttg aaaaaggtta
     4261 caccacaatc agtcctattg aaaaaggatt tgaaactggc aaatatgcca tgatgctgag
     4321 cggttcttgg acaatggcag atttagagga aaactatcaa gagattgatt atggtatttt
     4381 gccttacccc gtttcagatg caactgggga gttagtttca ccaaccggaa gctggcagtt
     4441 agcaatgact gagaaaacag agaaaaagga tgcttctgcg gcctttatta agtttgcaac
     4501 cagtacagaa tctagtcgta ttatgagttt agggaacagt gttctaccta tcagaaattc
     4561 aacagttgat ttaattaaag atgaagtgtc ggaaggaatg caggtattaa ttaagcaaaa
     4621 ccaaatttct ggacatgccc gcccagtggt tgtagcttat ccgcaagtat ctagagcatt
     4681 ccaacaagca gttcaagata ttagctactt cgaggaaaac ccagatgtac aaaaagtagc
     4741 agatagtcgc gctgctgaaa tgcaacaggc aattgatact tcactaaaat agaagtttct
     4801 aaagggggcc aatcaagtgt taaaaaaacc gacaatgaaa gacaatttga taggttacgg
     4861 ctttctagcg ccagctttgc tgctgctgac gatttttctg atcatacccg taatcatggt
     4921 aatttattat gcctttactg attattattt gttaatgcca gatgctagac aatttatcgg
     4981 cttagaaaat tttaagcgac tgttcgctga tccaattttt gtaaaaagta tttggaacac
     5041 ggctaaattt gttgtttgga ttattccggt ccaaataggg gcagctttag gaatggcact
     5101 tattatcaac aaaaatcgta aaggaaatat cttttttaaa gtagcctttt ttgcgccagt
     5161 cgtgatgtcc ttagtcgtta tttcgatttt atggttgtac ttgttaaatc ctaatgaagg
     5221 tctggttaat acgctatttg caaagattgg aattgcttcg cagccttttt tgactagtcc
     5281 caaacaagca atgtatacgc ttgtgttcgt atcggcatgg caaggtgcag gatatcagat
     5341 gctgctgttt ttagggggaa tgcaaaatat tccgcaagac gtttatgagg cagccgaaat
     5401 tgatggtttt tctaagtttc agcaatttcg ctatatcacc atgccattat taaagcccac
     5461 ggcgattttt attttactta ccacattgat ttcggccttt aaactaatca ttcagccaat
     5521 ggtaatgact caaggtggac caatgaattc tactatgaca atggtttatt acatttacca
     5581 aactggattt acagaccgta tggtaggcta ctccagctcc attgcgttaa tctttaccac
     5641 tatgattggt ttaattacaa ttgctcaaag aaaattggta aaggaggatg atccagcatg
     5701 accaagaaat ttggaccgct aatgatcgta gaatattgtt tgctgttcct tttagcggca
     5761 ttattcattt tcccgatgct gtggatggta gtttcctcta tgaagcctga agcagatgtc
     5821 tatcaaaatt tagcttcctt caaagctttt ttacctagtt tgaatccagc taattggttt
     5881 aaaacttatc aagaggttat ttcccggttc agtgtgggaa cttatttatt aaacagtatt
     5941 ttttatggtt taacctttgc ctgtgggtca atcattgtaa actctttggc gggttttgcc
     6001 tttgcgaagg ttaatttttc aggcaagaaa attatctttg gagctttgtt agctctgctg
     6061 attgtcccga tggagacggt tttaattccg caatttacca tcgttaattc actaggctta
     6121 gtgaatactc gcttggcagt gattttacca gcgatggcca gcgcgtttaa tatctatctg
     6181 tttagaaatt tctttatcgc gattccagaa gaaatcattg aatcagcgaa attagatggt
     6241 gcaaatattg ttaaaatttt ctttcacatc atgctgccaa tgtctaaacc agccatagcc
     6301 acagtaggtg tcttatcctt tatctccagc tggaatgatt atatttggcc gttaatggtc
     6361 ttaactgaca gcagcaaatt ttcaatgcaa gtggcaatta ctacgatcaa tactacacag
     6421 ccagtttata tcaatcaagt gatggctgtt ttaaccattg caacgattcc actgattatt
     6481 gtttatatcg ttgcacaaaa atatattctg cagggacttg gcggttctgg aacgggtata
     6541 aaataggagt gaatagtgaa tgttgcaaaa acttttaaag gccaatgagt ttattgagaa
     6601 aaatgctcaa acagtcaatc aagaataccg gaataaattt catttgatgg ctccgattgg
     6661 ctggatgaat gatccaaatg gttttgttta ttatcaagag gagtatcact tgttttatca
     6721 atattatcct tacgacagca tctggggacc aatgcattgg ggccatgcta aaagcaagga
     6781 tctaattcac tgggagcatt tgccagtagc actggcacca ggtgaaagct atgacaagga
     6841 aggctgtttt tccggcagtg ccatagaaaa ggatggcaag ctgtatttga tttatacagg
     6901 acacaatgtt gtggaagggc aagttcgaca agttcagtgt ttggcagttt cagaagatgg
     6961 cattcacttt gaaaaatatg ccggaaatcc aatcatcgca gaagatcact tagcttctgt
     7021 tgcaacgacc gaagatttcc gcgatcccaa agtgtttcag cgaaaagaag actattttac
     7081 tgtagtcgct actaaaactg cagatgctcg tggacaaatt gtgatgttcc attccaaaga
     7141 tttactaact tgggagtttt attccgtatt gctggaggga actccagaac aaggcattgt
     7201 atgggaatgc ccagacttgt ttgaactaga tggaaaagaa gtcttgattc tttctcctat
     7261 tcaaatgact ccacaaggca atgcttacca aaatacgaac tcaactgttg ctttcatcgg
     7321 tactgtcgat tggcaaacag gcaaatttct agtagagaat tttcatgaaa tcgatggcgg
     7381 tttagatttt tatgcacccc agacctgtga aaacgaagca ggccaaagaa taatgattgc
     7441 ttggatgcaa atgtggcagc gtaaaatacc tacgaatgat ctgggccatg gttggtcagg
     7501 ttccatgacc ttgcctagag agctgagggt ggctgataat caattgttgc aacggccagt
     7561 ttcatcaatt tatcaagtaa ttgatccgca ggaaaggatt gaatgggcga agactgaaga
     7621 gaatcgcttc ttactgaagg acttagtata tgaccagcac tatttacgct tggaaatcaa
     7681 tttaaaacag gcaacgaaac tagaacttca ctatgcgaag ggacccgacc atacattact
     7741 gcttaactat gatgttgaac gggagtgctt tactgcttcc agggaacaca tgccgtatga
     7801 tttatcagga gctgaaaagg aagtgttgaa aaagcgcagt gtgacgattc ctttggaaaa
     7861 taattgtcta accatcgaaa ttttccgtga tacttcagct attgagcttt ttttaaatga
     7921 tcgtctgact atgagtacaa ctttttatga gacagaaaaa ggacaggatt ttcttttgct
     7981 ggcagacagt gaagtaacct tttcattgga aagcggctta attaatctat agaagggaga
     8041 gctcagtgat gatttatggt tcaatcgaag caggcggcac aaaatttgtg tgtgccattg
     8101 gcgatgaaga aatgacgatc aaggaaagag tgagctttcc gacaacgaca cctgaggaaa
     8161 caataccttt agtcattgac ttcttcaaac aataccaagc agatttagca gggattggga
     8221 ttggctcatt tgggcctatc gatatccatc gcgattccgc cacttatggg tacattacct
     8281 cgaccccgaa attggcatgg caaaattttg atttcatcgg taccatgaaa aaagaatttc
     8341 cgatcccgat ttcttggaca acagatgtca atgcggctgc ctatggtgag tatgtcttcg
     8401 gcagtggcaa aggattgagc agtgtcgttt actatacgat cggtacgggt gttggcggcg
     8461 gtgccttaca agaagggcgc tttatcgaag ggtttagtca tccagaaatg gggcatatgc
     8521 tcgtcgtccc ttatccaaaa gatgactttg ctggcagctg tccatttcat ggcaactgtt
     8581 tagaagggat ggccgctgga cccgccatcg aaaaacgctt agggaaaaaa ggacaagagg
     8641 ttgcagaaga tgatccttat tgggaaatcg aagcctccta tattgctcaa tgtgcctaca
     8701 atacaacctt gatgttttct ccagatgtga tcatttttgg cggtggtgtg atgaaacaac
     8761 gccatatgct gcaaaacgtc cacgacgcat ttgcaaaatt ggtcaatggg tatgtcgaaa
     8821 cacctgagtt aagcaaatat atcgtgaccc cagcattaga agacaacgct ggaacgctcg
     8881 gttgtttggc attggctcga gaagcggtct tacattcata aacgaaaaaa ggctcttcga
     8941 catttagtgt tggtagagaa agatacaact gaaaatttag tcataaccag ttaggcagag
     9001 tgcttaactg gtttttgttt tacttggaag atcaagcctt gaatgagata agttcttgct
     9061 cgaaagttat ggaaatttcg atagccgtag gctacttgta ttgtgcaata gttttttaca
     9121 aaaaaaaggg ggtgcggata ttttttttgg acctttttaa caaatatatc tttgcttctt
     9181 ccgatattga agagggctta gccctcctaa agataactta attcgttgat tattatacca
     9241 atgaatataa tcatccaatt tgtttataaa ctgattgatt gttgtattct tccagtcacg
     9301 ttgataaaac atctcgtttt ttaatcgtcc aaagaaacct tcgcacgctg aattatctgg
     9361 tgaataaccc tttttagaca tagagtgaag caagttggct ttgttccaat agtccaacta
     9421 acaatagcac catcgtaaca atcgacaagt ggtgataagt agattttccc tgcgagaatt
     9481 ctaaattcag taatatctgt taaccatttt tcatttggct tctttgcttt aaaattccga
     9541 tttaataaat tagggtgtgc aggagaaatt tccccagcat acgaagaata cttttttcgt
     9601 ctagaaaatt taacaaatag attttcctca gtcatcaatc tacgtacaac tttttcagaa
     9661 agctttttac ccatattttt taaagcttgg tgaattctac ggtaaccata cgcacagaag
     9721 ttatgattga aaatcgtgat aattaatttg acgttctttg taatacttat ctggtttctc
     9781 aagtgcttgt ttttgataaa aatagctgct tttaggaaac tggagctgtt gaagaagctc
     9841 tttcaaggtg aactgatttc gtaaggcatc aatcaatatc gttttttctt gatttgatag
     9901 acaaagaaga ttgacgcctt tttctttttt tagcagttca gccgctcctt cgagtaatgc
     9961 tttttcgagc tgtaattggt gtacttgttt ttgtaagtct tctacttcct ttaaaagtag
    10021 ctctttttgt ttttcctgat tttttaaagt catacacaat ggaaaatcct ttccaagaac
    10081 cgtgtttttc cattgatata gagtaactct actaattcct ccagattcgg ctaaattttt
    10141 cctattagtt tgcggtttat agaatttttt caacacagct tctttttgat cttgtgttaa
    10201 ctttaccgca ctttttcgga gttttctagc tcctggtgca agttcttcac accgctgccg
    10261 caaggactct ctattaggat aaccaagcat gcgaatggtt cgtgcataac attgaccata
    10321 ttcaaaataa tgattcaccg ctattcgttt ttgttcttca gaatactttg atttttttcg
    10381 gtgctcctga tggagctctc cggattccaa atactcttta taccatttcc gtagtgcacc
    10441 aatcgacgga taccctaatt ctcgaatggt tgcagcataa gagcaatcgt attttaaaaa
    10501 taactgaatt gcccttacac gctctttgtg ggaaaacaca cggacattcc tcctaagttt
    10561 attttttcgt ccaggattat gtccgcaccc ccttgctttc tgatgactta ttctggttct
    10621 ttgagtgtca atgcatagat gtgatgtagc tagttagaaa aaaattttga atactctttt
    10681 tttctttcag actccttttt aagtaagatt ttgaagtgtg agttacgggg tcagaaactt
    10741 aaaagttttt gatcctttat tttcaagtct gatttggcag cttttcggca gctcatttat
    10801 ttatggtcag aaatagatgc tgaaatttta atctattagc aaatagtcca gaacgtatga
    10861 agaggagtcg gatatcattt gtcattaaca ccataaatat ttataaaatt cgtgttcacc
    10921 tacaattaaa tttaatatat aatattcgtt gtaagggctt ataatttttt gttaatgaac
    10981 aagataggaa gaaacattta atggacagtt ttcagagatt gtcactagta aatcaatttg
    11041 agttattggc taaatttgag gaccaaaaca gatcttatta tgaaagaaaa atcgaaatat
    11101 tgagagaagg ttacgagtat cattatgatg atgaaatttg gagtgatctt tctgagccat
    11161 ttccgaaaga agattctcga tttgtattag atgtattgaa catgtatcga gatatcaatt
    11221 ttagtaaatc aaagctaaac ttagatgaaa accaaattac taattcatac tatactcatt
    11281 tcggttctgt tgcaaagttt tccaaaaaat ctattttagt gtaaaattga gaaaaaag
//
LOCUS       DAFEPQ010000076        10961 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.078, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000076
VERSION     DAFEPQ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.078"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(66..1622)
                     /gene="rny"
                     /locus_tag="LP543_002271"
     CDS             complement(66..1622)
                     /gene="rny"
                     /locus_tag="LP543_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBL2322414.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(2033..3082)
                     /gene="recA"
                     /locus_tag="LP543_002272"
     CDS             complement(2033..3082)
                     /gene="recA"
                     /locus_tag="LP543_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBL2322415.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(3191..4432)
                     /locus_tag="LP543_002273"
     CDS             complement(3191..4432)
                     /locus_tag="LP543_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBL2322416.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(4672..5250)
                     /gene="pgsA"
                     /locus_tag="LP543_002274"
     CDS             complement(4672..5250)
                     /gene="pgsA"
                     /locus_tag="LP543_002274"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBL2322417.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(5459..6382)
                     /locus_tag="LP543_002275"
     CDS             complement(5459..6382)
                     /locus_tag="LP543_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2322418.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRVFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(7121..8407)
                     /locus_tag="LP543_002276"
     CDS             complement(7121..8407)
                     /locus_tag="LP543_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBL2322419.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(8400..9668)
                     /locus_tag="LP543_002277"
     CDS             complement(8400..9668)
                     /locus_tag="LP543_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBL2322420.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            9920..10381
                     /gene="mscL"
                     /locus_tag="LP543_002278"
     CDS             9920..10381
                     /gene="mscL"
                     /locus_tag="LP543_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBL2322421.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(10440..10886)
                     /locus_tag="LP543_002279"
     CDS             complement(10440..10886)
                     /locus_tag="LP543_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2322422.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         2980 a         2390 c         1932 g         3659 t
ORIGIN      
        1 ttttcgtacc ttacaaaaaa agaagagctg tggcttttgc cgcggctctt cttttgtatt
       61 ttttattatt tagcataatc agttgcacga gtttcccgaa taacgattac tttgatgtgt
      121 ccaggataat ccagttcttc ttcgattcgt ttacgaatat cacgtactaa acgaacagcg
      181 tctaaatccg agatttcttc tggcttgacc attacccgaa cttcgcgtcc agcttgaact
      241 gcgtaacttg attctacacc ggcgaagctg ttcgaaatat tttccagact tcttaaacga
      301 cggatgtagt tttctaatga ttcacttcgc gcaccaggtc ttgctgctga gagcgcatct
      361 gctgcagcta caaggacaga gataacagaa gtagcttcca catctccatg atgagaagcg
      421 atcgcgttga ttactactgg gttttctttg tacttggcag ctaattccgc accgatctca
      481 acgtgagatc cttcaatctc atggtcaagt gctttcccaa tatcatggag caacccggca
      541 cgttttgcta attggatatc ttcgccaagc tctgctgcta agataccagc taattttgca
      601 acctcgaccg aatgcttcaa gacgttttgt ccataactag ttctgaatcg cagacgtccg
      661 atgatcttga ttagatctgg atgaagtgta tgagcaccga cttcaaacgc tgcctcttcc
      721 ccgtattcgc gaatacgttc atccatttct ttacgagatt tctcgaccat ttcttcgata
      781 cgcgctggat ggattcgtcc atcttgtatc agtttttcca aggtcatccg tgcgatttct
      841 cttcggattg gatcgaatcc ggaaagaaca actgcttccg gtgtatcatc gatgattaaa
      901 tcgataccag tcagcgtctc aagcgttcga atattacgac cttctcgtcc aatgatacga
      961 cctttcattt catcattcgg aagggtaacg actgaaacag tcgtttctga aacttgatct
     1021 gctgcgcagc gttgaatcgc taatgataat aagtttttcg ctttgcgatc tgcttcttct
     1081 ttggcacgct gttcagattc ttttaccatt agagttaact catgattcag ctcttcttca
     1141 gtcgatttca tgatgacatc tcgtgcgtcc tcttttgata aaccagcaat tttttctagt
     1201 tctttttgtt gatcttcaat cattttttca acgtcttttt ctcgctcttc aattaattgc
     1261 tgttttgaac taagtgcttc ttctttactt tcaagtgagt tctcgcgttt ttctaacgag
     1321 tcatctttac gattcagcgt ttgttcgcgt tgaatcaaac gattctcttg agattttaat
     1381 tctaacttac tttctctcag ctcactttca acttcagaac ggtatttctg attttcttcc
     1441 ttagcttcta acaatgcttc tttttttaga gtttctgctt ctttttttgc actatcgatg
     1501 attccagcag ctgatgattg tgcaccagct atttctttct cgtgacgtga ctttgcgacc
     1561 ataaacccta aaccaagacc gacaattaaa ccgatgatag cgaggagaat gttgaatacc
     1621 ataaaatcca cctccatact atctttttca tttttatcat ctatacagct tctatgataa
     1681 attattctaa actcgtcaat atgcatcttc gcatatatgt gtacaaaaaa catgcacaac
     1741 tttattctaa tgtttgtacc ccgtagtgtc aactttaaat aaacaaagaa acggctttct
     1801 acttattcat taaacgatgg tttttaaaat aaatgacact agtttcatat aggtaaacct
     1861 ttaaattgag aaacttttct attaaattat tctttgtaga tgaacagata tttttatttt
     1921 atgtacccac tcgtataatt aaataactca cgatttcttt attcaataaa aaaacggcag
     1981 gtcaaggaga gtacctactc ttcagaccag ccgtttttgt ttatcataat ttttattctt
     2041 ctactaaagg caattcttct tgtccttttt catcttctgg gacagcgact tcttcaccaa
     2101 taccataagc tgctctaact agagcagaaa cttctgccat catttcagga tgattagcca
     2161 tatagatttt agcgttttcc cgtccttgac caatccgatc ttctttataa gaataccaag
     2221 caccgctttt atcaacaatg tctttttcta ctgccatgtc tagaagttct ccttcttgtg
     2281 agatccctaa accatacatg acatctactt cagcgatttt gaaaggaggt gctactttgt
     2341 tttttacgac tttgatcttt gtacggttac caacaatatc tgtaccttgt ttcaattgtt
     2401 ctgcacgacg tacttccaaa cggatcgttg cgtagaattt caatgcgcgt cctccaggag
     2461 tgatttctgg attcccaaac atcacgccta ctttttcacg gatttggttg atgaaaatag
     2521 cgattgtttt tgttttgttg atcgaaccag agagtttacg caatgcttga gacatcaaac
     2581 gtgcttgtaa cccgacatgt gagtcaccca tttcgccgtc gatttctgct cgtggaacta
     2641 aagcagcaac tgaatcgact actacgatat ctacagcccc acttgatact aaagcatcag
     2701 cgatctctag accttgttct cctgtgtcag gctgtgaaag aagtagttca tcgatattca
     2761 cacctaattt ttgtgcatat tgcggatcta acgcatgctc agcatcaatg aaagcggccg
     2821 ttccgccatt tttttgtact tctgcaatag cgtgtagtgc aactgttgtt ttacctgaac
     2881 tttcaggtcc atatacttcg atgatacgtc cacgggggta accgccgaca cctaaagcga
     2941 catctaacgc aagagagcca cttggaattg tagagatttg ttggtcgatt ttttcgccca
     3001 atttcataat agagccttta ccatagctct tttcaatttt tttcagtgca gcatctaagg
     3061 ctgctttacg atcatctgcc aatacgataa tcctccttat atttacaaat cagagcaaca
     3121 tttcattatc cttatcatat cttttttagt gaaaaaaagc aagaaaaaac cgaacaaata
     3181 ttcgcattta tcattttctt aaaagctgtt ttctcaaaag atcaaatcct tgcataaccg
     3241 cattatatct gatagcagca cgatctctgg caaagtgata tttttccgaa accacgccgt
     3301 ctttagatga aatagcgatc catactgttc ctgcagattg accttctaac gtgtccggtc
     3361 cagccactcc ggtgaaagct acactgtaat ctgttcttgc tatctcacgt gcttgaatgg
     3421 ccatctgctc cacacattct ttgctgactg ttccatattc tgctaatagc tccggatcaa
     3481 tctccagaaa accagctttt gtttggagcg agtaggtaac gaatccgcct ggaaatactt
     3541 cagatacacc agcaatgtcg cctaatgcgg cttggaaggc gccggctgtc aaactttccg
     3601 ctgctgttac agtctgcttg ttttctttca gtagttccac tacgactttt gctagcgaat
     3661 tatcatcgcc gtatccataa aaatattccc ctactcgttc ttggattttt tcttctaatg
     3721 ctaaaagagc ttgattccct gcctgaacat catttgtttt cactgtcagt cgtaatgtca
     3781 cttcatttgg tttggcatat ggtgcgatcg ttggattgat ctgtgtctca atcagatctt
     3841 ttagctcagt gaccagtcgc gattcaccaa tcccataaaa gcgcaaaact ttagaaatta
     3901 gtttttcttc agaaggaaaa cgttcctcta gtaaaggacg tacttgttcc acaaacattg
     3961 gtttcaactc attaggcgga ccgggaagaa ggatatacgc atgtgtatct gtttgataaa
     4021 aagtccctaa agcaagccct gtacgatttg gtagaggaac cccgccttcg ataatctggg
     4081 actgttgcag attgtttttg gtcatttttc gatgggttgt ttcaaaatat gccaataatt
     4141 ttttgtatcc ttccgtattt tgtatgagag acttaccgag gtgagccgca gttgcttctt
     4201 ttgtcaggtc atcttctgtc ggtcctaatc cgccacataa gagtatgagt tcacttcttg
     4261 tttcagctag agcaatcaac tcttctaaac gctgctgatt atctcctaca actgtttgat
     4321 aataaacttc gatacctaga tcagctagct gttctgacaa gaaagtggca ttcgtattca
     4381 cgacttgtcc tagtaaaagt tctgttccga cagctattat ttctgctttc attacttccc
     4441 gctcccttat atagtatacg ctgtattttt atattttttt atttaaaaag agggcaagga
     4501 gctgtgacaa aaatcttgtc acagctcctt gttccaaata aacagtgttc tcctgccaaa
     4561 acctcggtca taagtcacaa tatacataaa acatgaaaag ctgttttttg aaattatccc
     4621 ttgtgactca gagttagacg gcaggctcac aacctctcca ctaaacgata cttacattga
     4681 tcctttgaac acaccgctgt ttttgatgaa gtaatccaca cctgaataga tagtaaagat
     4741 cagacaagca taaagcatga tttgatctac tggaataccg atcaggctga atgggatatt
     4801 attgataaac agcaagataa tagcaaccat ttgtgtagcc gtcttgactt ttcccggcca
     4861 agctgcagcc atgacttctc catgttcgac aagtagtaaa cgcaagcctg tgacggctaa
     4921 ttcacgacag acaatgatcg ctactaccca accaggggcc tttccttggt caaccaacat
     4981 gataaaagca gtcataacta gcattttatc tgccaacggg tccgcaaact tgccaaaatt
     5041 agtgacaagg cctcgcgcac gggcaatttt tccatctaac cagtctgtga tactagctaa
     5101 agcaaaaata actgcaccta ctagctgtgt gacagccagt gtggtgtctc ctacgttaaa
     5161 ttgtccccaa tctaaaggta aacctacaac caaaatgaaa atcgggatca tacaaattct
     5221 aagtacagtt aatttatttg gtaaattcac actatcgact cctttcttcc ctattctacg
     5281 tgactatcag cgaagtttcc accgatatta tttacttttc caaatgaatt tattcaaagg
     5341 agtattttta ttgaaattga tctttttgaa ttaacgaaga aggaccgcga cacagatttg
     5401 tcacgatcct tcttttttat acctacaagt aaacagatgc gtaaactaat ttatacgatc
     5461 attctgcgta ttgaagagta aggttcacat tttttggact ctgattgttt tcccctggat
     5521 taactggtac ggattctcca ttcgctttga tcgttgcatt gcttgctgca ccaatcgtga
     5581 tggtcgcatt tgttgctgtt tccggtaaca cggtactctg tgtttcattt gctgccagtg
     5641 tcccttgata aacataggca ttgtctacta gcacacctaa ccatacacga ttgacagcag
     5701 taaattcaag ggttacaggc ttggtagcgt ctgtgatatt tactgcgatg gcactgccag
     5761 aatcttctcc tgtagctatt gccattttct tttcttcact tgattttgtt gttgattcta
     5821 ctgtagaaga ctctttggtc tgcgtgcttt gctctgagga agaggactcc tcgactgatc
     5881 cttctacttt aagcgaagaa gctgtgtctc cgattattgg atcagcttga cgatcttgcc
     5941 aagtcatata gcccacgaca acgacaatcg ccaaagcaac caatcctaat aaaatgacag
     6001 gaagacttgt ccaaaagcga cttggatgcg attcttcctg atgcattgct tttctagaag
     6061 tacttactgt ttcaggctgc ggacgttcag ggaccgatcc tgtcaatttg cttttcccat
     6121 caaaaacatc gactaaatgg tctccatctt ctcctacagc ttcagcgtat tgccgaataa
     6181 atacccgcac gtaaaattta ccaggcaatt ggtcaaaatc atcattgtcg attgcttcca
     6241 aataacgttt ttgaattttt gtaatctgct gtaattcatc taaagatatt tttttattca
     6301 ggcgtgcttg ccgtaatttt tctccgatgg tttcaaaagc cactcgttca tcttctccag
     6361 tctgtttgat tctccgaagc atctgctgtt gcaacgaaag acgttatgtt tgggtgctgt
     6421 gacaaaagtc atgtcacagc tccttttttc cgaataaaca ttgttctcct gccaactcct
     6481 cggtcacaag tcacaatatc caataaatat aagaagctgt tttttgaact tgttccttgt
     6541 gactcggagt taaacggcag gctcacgacc tctttcttaa acggtgttca atcagctgtt
     6601 cctcggtatt agctcaaaaa ccggcaaaat ataaggagat atttttccgt atgtttctct
     6661 aatactcagg tcaaaacgct gatttcacaa cctcttcctt aaacggtgtt caaaagcttg
     6721 catctgcggt aataagtcca accaagttaa aaacattaag aagctgtttt tacttggctg
     6781 ttcttattac ttgatgcagg gcagcttttt cacaacctct tggtacacgg tgttccagaa
     6841 gtagcttctt cggcaataag ccgaaatatc acaaaaattt gagaagcaat tttcgtaaat
     6901 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tctttgatta cttcgtttga
     6961 ttttatttta atataagttg attaaaagag agcatgattt gttttatttt ctcactaatt
     7021 tttccaacac aactaagaat accataaaag ctgattttgg aggaggttcc tactacaagt
     7081 ctaaaacgaa atttaagact tttcctcata aaatactggt ttattttggt ctcatgtaaa
     7141 actcgctgaa ggcctcttca cttataaatg cttccccagc tgccagtacg tctttcattt
     7201 ggatcgaatc gatgatttca ggaagatcaa ataaagttcg atcgccaaat aaactttgcg
     7261 taaattgatt ggcaatatat tcgatcgaat ttaatgattg gaaatattgt ccaagcattt
     7321 tcttcttcag caaatcgaga tttgtttctg acacttccgg atcatcagcg aatccaagaa
     7381 tgatttcttt gactttttct gctgctttct ctggttcgtc cgtatctccg ctgaagtccg
     7441 caaagtgaaa ttcacgatcc aagctgaatt caaaaccaaa gctgtcatcg ataattcctt
     7501 gattgtacat cgctagatag tttcgagaag tattgcctaa aatcatttgg aacaacagat
     7561 tgattgctgt tttatagcgt aacagttctg ttccttcttg cggcaatgtg tctaatcctt
     7621 taataccaag tacaaattta tctcgtgtaa tggctgcttc caatgcactt tgtttgatga
     7681 tttcttttgt gttttcaggg aaataacgta caatttcttg ctttggaggg aaattttttg
     7741 cttcttggtt ttcccgaata agtttcatca atttctcagg ctccatctta ccaactacaa
     7801 ataaaaccat attgcttggc tgataaaacg tccgatagca cgtatacaag tcttgagctg
     7861 tgattttgtc gatactttca accgtgcccg caatatcgat atgcaatggg tgtgtaggat
     7921 acaagttatt tagtatgcca aaaaacatcc gccagttagg gtcgtcttca tacatttgga
     7981 tttcctgacc gatgattcct ttttccttgt ttacagtttc ttctgtgaag taaggtgctt
     8041 gtacaaaatc gatcagagtc gtcagattct tttctacctg atccgtcgta gaaaacagat
     8101 agctggtttt cgtaaaactt gtgaaagcat tggcggaagc tccttgtttc ccaaataatt
     8161 gaaagacatc accatcttct ttttcaaaca acttatgttc taaaaaatgg gcaattccat
     8221 ctggaacttt caccttttct ttttcaccat agggaataaa ttcgttatcg atcgaaccat
     8281 aattcgtact gaacaaacca tatgtcttgt gatagtcatt tttaggcaac aaatacacgg
     8341 tcaaaccgtt tggcagtact tcatgataaa gtgtttcatt gatttgctca tattctgttt
     8401 tattcatttg catttcctcc tgctaaaaag aagattgcct gcaattcgat ttgctcagct
     8461 acttgttgta tctctttgat cgttacgctc tccatacgtt tcaaccattc ttcatcagat
     8521 aattttgttt ccggcagcca gctgtccaaa aaagaagctt cgattgccgc ttgcggagag
     8581 tctagagaaa gcagatattg gtttctcagc attgcttttg tctgagacag ttccaactct
     8641 gttatttcac catttcgcaa actttctaat tgctcgtgaa tcagatgaag tacttgattt
     8701 cggttctttt catcgatacc ggtttggaca ctcatgaatc ctcggaacgt atccacgcta
     8761 ctagaggcat aataagctag actttctttc tctctgacat tcatgaaaag tttagagtga
     8821 ggaaaaccgc caaataaacc gttaaagacc attagcgcaa aacgttctgg ttcatcgtaa
     8881 tacacatttg tctgataagc taggttcagc tttgcttgaa cgattggctc acgtacttgt
     8941 cgttccttca cgatattcac ttgaggttgg gtataaaaca gatctggata aactgtttcc
     9001 cgcgactcaa atggtaattg cccaactgct tcagctgctt tgtccggatc aacatcgccg
     9061 acaacaaaaa tgtcgatttg atcatttgcc atcatttctt gatagtagga cgctagcgaa
     9121 ctagcagtga tcttgtccaa atcttcgact acgcccaacc ctgggatctt ttgttccggg
     9181 ctgttttcaa aataaagttc ttgcaaggca taggaagcaa aagtttgttt atcttccttc
     9241 aaactttcca aatatagacg cagattgttc ttttccaagt caaacgtcaa ctgatcaaac
     9301 tgatgatttt taatatttgg ataaaataaa acctctttta aaaaatctac cgcttgagaa
     9361 aaaaggtttg gatcattgac atattcacca ttcacaaaac tgatccctgc gtttagccaa
     9421 tgtaaatttc ctttttttct aactgataat ccaaagcttg cgccataaag ctctgcaagt
     9481 tgcgcactta actttgtctg atccggataa ttcaaactat ttgtttccaa catgctgctg
     9541 agaagtgaac gtttcgtgat gacttcttgt tgcagacgcg cagtaaaacg gataaaaatc
     9601 cggatcgttt tgtattgttt tgttggtaag acatgaagat tcacgccttc tgccaattga
     9661 tacaccatat ttatatgctc cttttcacag ttgattgccc gcttactctt ttaatgggtc
     9721 aacctgcctt ttttacttat tcctctgctt gaaatttata agttcaggca attcgccgtt
     9781 ttttctaaat gatgttacac tttatagtat agcatagatg ttttttatca aaagaaacag
     9841 acggctaaaa gcgtatattg tttttttatt ttttattttt cgatatacta aagacaaata
     9901 aaagaaggga gggtcattca tgatcaaaga attcaaagag ttcatcatgc gtggagatgt
     9961 cttggatctt gctgtcggcg ttgttattgg tagtgcgttt acaagcatcg taaaccaagt
    10021 ggtgcaaggc ttgatcacac cactaatagg ttggtttgtc gcaatgatca caggtacttc
    10081 tgatttagaa ggagcacttt caattcttga ctggtcgccg acaaaaggcg tcacatttgc
    10141 gtttggtaac gttattagtg ctatcattac atttctgatc actggttttg tcttattctt
    10201 agttgtcaaa gcggcaaacc gtgcgaagaa tttacgctcc aaagaagagc aagtggaagt
    10261 ggaagaaact cctactgcag aagattatct tagtgacata cgcgatcttc ttgccaaaca
    10321 agtggcagaa gacaatacac atccagtaga gcaaacaaat ctttccgata cttctaaata
    10381 ataaagaatc taaaaaaaag aaagccgatt ttcagtgtcg gctttctttt tttagattct
    10441 taacttgcta cttgttcctt tttcatgtcg atatgtggaa tatcatcttc caaatacaca
    10501 tctgagattg ccttgaatcc aaacgactca taaaaagctt gtaggtattc ttgtgcgccg
    10561 atcacgattg gtttttctgg aaaatttttc tggatcatgt taatcgtttc ttgaacaatc
    10621 tttcttccta aaccatcttt acggttgtcc tgatgaacaa ggacacgtcc aaaatggata
    10681 ctctccgctt cttcatatac tcgcgtgtag gcaaggatct tcccatcagt atcttgaaaa
    10741 aacgtatgcc aagcttgtcc atcaatgtca tcaacttctt gataaggaca ctgttgttcc
    10801 acaacaaaaa ctgcgattcg ttctttgacg atcgcgtaaa actctgctgt tgacaattct
    10861 tcaaatttct ttacctgata ttccactaag caaacgcccc ctgctttttt attcttagca
    10921 taccgattcg cacacaaaaa agcaaacgaa ccttacgaat c
//
LOCUS       DAFEPQ010000077        10715 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.079, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000077
VERSION     DAFEPQ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.079"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(<1..537)
                     /locus_tag="LP543_002280"
     CDS             complement(<1..537)
                     /locus_tag="LP543_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL2322423.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHFVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFKRNISERKKDYVVFEEEF
                     GHLEGDTIVGIHHKSVVIT"
     gene            complement(619..969)
                     /locus_tag="LP543_002281"
     CDS             complement(619..969)
                     /locus_tag="LP543_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HBL2322424.1"
                     /translation="MINNYLHYKAINWNVIEDELDNVVWERATSLFWLDTRVPIENDR
                     SKWANLQLQEQEQLNRLLILLTNIATYQSNELGEIIRDSARSQQEIAIINNFQFTEMV
                     HTKAYNRILSIRQF"
     gene            complement(1018..1461)
                     /gene="nrdI"
                     /locus_tag="LP543_002282"
     CDS             complement(1018..1461)
                     /gene="nrdI"
                     /locus_tag="LP543_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBL2322425.1"
                     /translation="MQIIKVYYISLSGNTTSFLADLDTYCQKQYQKKVDYVNVKDLVK
                     LGKPLTFDINESYFVFLPTYLEGGNGIETGDVEILTTPLRQVITYNKNRDYCLGIIGS
                     GNRNFNKQFCLTAYQYADDFGFTVLDEFELKGTTEDIERIAETMQ"
     gene            1608..2273
                     /locus_tag="LP543_002283"
     CDS             1608..2273
                     /locus_tag="LP543_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL2322426.1"
                     /translation="MKNSGCLCTVPLFASLSLDEKNEIKCLLHHQTFKKGEIIFSPSS
                     EEQLLIISLGAMKIYRISRTGKEQLIRIVKEGDYDGENYLFGLPNDCLYGEAVEETKT
                     CTLYKSDFNKVMARYPQLSYRLLQLNACKTVAVEKQLQLLAFDRIEDRLALYLNQLSI
                     AAKMGEPITLPISMRELANYLATSPETISRKLRDFQERQLITRKGKQITLLEEFWFIF
                     NYI"
     gene            2263..2718
                     /locus_tag="LP543_002284"
     CDS             2263..2718
                     /locus_tag="LP543_002284"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010905674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBL2322427.1"
                     /translation="MIYKEEDIKIGIVIARFNDFLTKSLLSGCEDGLTRSGVLTENID
                     VAWVPGAYEIPLTAATMAETGKYDAIITLGAVIRGATSHFDVVVNQASSGVSQVGLTH
                     KLPVIFGILTVENIEQAIERSGTKAGNLGFTYATNTIEMINLFKEIKQQ"
     gene            2868..4247
                     /locus_tag="LP543_002285"
     CDS             2868..4247
                     /locus_tag="LP543_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HBL2322428.1"
                     /translation="MTIQFKQTLAPTKDNSGKPIILSDATFAQRNEKVLQLMREYDFS
                     SLMIYADKEHGSNFEYLTGFIPRFEEAIQVINQDGTSTLILGNENYNKTSMARTQSEG
                     ILCPFFSLPNQPMDFTKTFADYLKDVKVDASKRIGLVDWKLLSNDMDNFHNHHSIPAF
                     IVDGIHEVFGSDKVVNATQLYMHPKEGARVTNNAEEIARYEYGASLASDAVLEAMNQL
                     TVGQSELAAGNHLNRDGQYPSVVTIAAFGDRFEGANIYPTDNTLNDGDKVALTVAYKG
                     GLSSRSGYAVKEESQLAEHDPGYLEEVVYPYFQAYKWWLDNLTIGRKGGDFYQAFSDF
                     YPQATYGWNLCPGHLVADEEWLSSPFYQDSDATIQSGYIFQLDFIPSQAGHNGVSAES
                     TIAVADAALREQIKAEQPELWARICNRKDYLKEALGLELSEHLLPLASTFAYYRPFLL
                     DKKTAIYFG"
     gene            4384..5226
                     /locus_tag="LP543_002286"
     CDS             4384..5226
                     /locus_tag="LP543_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBL2322429.1"
                     /translation="MPLDEQIELLFTIDEGYLNPLKVALTSIRQNNPGQAFRIWLIHE
                     SIAMHTIRELQKLTDYLQFGFEAIKIDGSRWNSAKTEDRYPKEMYFRLLAGEILPKEM
                     KRVIYLDPDILVVNPLLDLWQTDLEGYMLAAATHVGLTDVSTRVNQMRLDVDHAYYNS
                     GVMVIDLDKAREIIKWSDIAQMIEKYNLLLILPDQDILNRLYGKYTKEIPEEIWNYDT
                     RKYMRYFTKSLAQHDIHWVMAHTAILHFCGGPKPWDDKHDNRFTSLYLTYQNQLKRIE
                     DLMK"
     gene            5300..6748
                     /locus_tag="LP543_002287"
                     /pseudo
     CDS             5300..6748
                     /locus_tag="LP543_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            6863..8635
                     /locus_tag="LP543_002288"
     CDS             6863..8635
                     /locus_tag="LP543_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL2322430.1"
                     /translation="MNNNETKKTYELRITADEIKSTDPTFTMKNLSLGDNYQPKDAYF
                     RTAEGEITRRNYGKNERKISNQTLPRLAIQMFEQSIVSLPESERATFPVCRYHSGDEI
                     IRGIFPDVDSFKKKRKSIEFLIYKYNEDKQIVIYCWSIFSTLLFVQECLKRFGTSGDE
                     FILVYKGKEKKEIRVGETQTMTNHLLKSYVLHLLKSKNLILRGAPGTGKTFLANEMAA
                     DVVSNGRTTQINELSEDEKSRIGFVQFHPSYDYTDFVEGLRPTTSDDGVVSFTLKDGS
                     FKSFVEKAKKTKKVNGQDNFEEAWAKFFDAVTEASIEGEGYNKLKTLTGKPVRNLLAY
                     ERNEIQGVYPAKTNTYLNHDQIYNVYRGLPGTPKGGFDAYRKAIVKHLKEEFGLKNYT
                     ASTVDSTNNQNYVFIIDEINRGEISKIFGELFFSIDPGYRGHKEGVYTQYANLHENPE
                     EKFYIPNNVYIIGTMNDIDRSVDTFDFAMRRRFTFLEIKAEDSAQNMLTQEKTKELMT
                     RLNEAIVAKEKGGLSTDYQIGASYFLSIDNGEETMEQLWDTKLYPLLKDYFHGEHKAK
                     EKLEILEKVYSNDGDLDEAETSTR"
     gene            8610..9902
                     /locus_tag="LP543_002289"
     CDS             8610..9902
                     /locus_tag="LP543_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine 5'-monophosphate oxidoreductase"
                     /protein_id="HBL2322431.1"
                     /translation="MKLKPLHDNQEINDSVVQHPIITNLVNRDLSSLEKENFIIFPQQ
                     LNQSKDLSEDNLIFQSRNGKIWTCNVVGILSDSTDELRIHSRFANQMKHEDFFLRYML
                     QRVLNYNVINDHFSASKKMTYYDLLVFLFPYYLNEAMRKGTYKEYVKREYNNANIKGA
                     VDVAKHIRSNVPFVGKVAYRTREFSYNNHLTQLIRHTIEKIQNEYDFLLSGDEDTKEN
                     VVLIKQTTPGYARLNQFNILQENILHPVKHSYYEEYSALQQICIQILSEEKSGFGSDK
                     NQIHGIIIDVSWLWEEYIGKVTGWKHYGRDKGLATMHLFQEPNRSPRYPDFTFNNIPI
                     DTKYKKHLDTRNDYNQLVTYIHIMNLDQADTMKGGFLQPTSDPLPANHGYKKIGILSG
                     LGGEIFTYHFFIPQAASSYLEFVEQMREVEAALQTTFQ"
     gene            complement(10365..>10715)
                     /locus_tag="LP543_002290"
     CDS             complement(10365..>10715)
                     /locus_tag="LP543_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL2322432.1"
                     /translation="LETLSEEANLPNILGDKGYISHEIHEKLALKGITISVPPRKNMD
                     KSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRA
                     QRRFEGTLRYSLGY"
BASE COUNT         3670 a         1762 c         2019 g         3264 t
ORIGIN      
        1 tgtgatgacg acacttttat ggtggatgcc gacaatcgta tcaccctcta aatgtccaaa
       61 ttcttcttcg aaaacgacat aatctttttt acgttcagaa atatttcttt tgaatgcctg
      121 ttttcctctt ttttctttat gtccgtttgg ttttcttttc cctttcattg gtaaatcgtg
      181 agggtcaaag gttttatctt taaacatccg atatagtgta cgtaccgaac aagcaatagg
      241 aaactctgct cgaccaataa ggacatctgg agtccaccct tgagccacct ttttttgaat
      301 gtattcttgt tgcccatctg gtaacacaat cggacgtctt ccgcagttac gtttgttttt
      361 cttatattgt tcatagtagc ttattgcgct tccaccagat ttaagaaaac gatagacatt
      421 ataaatggtt tgtcgagaac gttgtactag atgggctact tttgaaacaa agtgtgattg
      481 attgaaataa gcttctatca tcacgagttc agttggtgta agatgggtat aggtcatttg
      541 tactcactcc ttataattct ttcgacggaa ctattttgag tgtagcacaa atgacttttt
      601 tagttgtcta gcttaatttt agaattggcg tatgcttaaa atgcggttat aggcttttgt
      661 atgaaccatt tcagtaaatt gaaaattatt tatgattgca atctcttgtt gggaacgtgc
      721 actatctcga ataatttctc ctaattcatt ggattgatag gtagcgatat tagtcaaaag
      781 aatcaacaag cgattgagct gctcttgttc ttgtaattgt aaatttgccc attttgatcg
      841 atcattttcg atggggacac gtgtatccaa ccaaaaaagt gaggttgctc tttcccatac
      901 aacgttgtct aattcatcct caatgacatt ccagttaatt gctttatagt gtaaataatt
      961 atttatcata gtacctccta aaatattgtt tgaaagggga tgatatggtg tcatttttta
     1021 ttgcattgtt tctgcaattc tctctatatc ttctgtcgta ccttttagtt caaattcatc
     1081 caacacggta aaaccaaaat catcggcgta ttgataggct gttaggcaaa attgtttatt
     1141 gaagtttctg ttgccactac caataattcc taaacaatag tctcggtttt tattgtaagt
     1201 aatcacttgg cgcaatggtg ttgtcaaaat ttcgacatct cctgtttcaa taccatttcc
     1261 accttcaaga taagtaggca gaaatacaaa ataggattca ttgatgtcaa aagtgagtgg
     1321 ttttcctaat ttgactaaat cttttacatt cacataatct accttctttt gatattgttt
     1381 ttgacaatac gtatctaaat ctgcgagaaa actcgtggta tttccactta aactaatata
     1441 atagacttta attatttgca tgtatcacac ctctttttaa taaataacca catatatagt
     1501 gcttgtttta attagaaacg ctatatatag tgtttattgt acatgacata tcgttataga
     1561 aaattgacga tggtcaatta ttttgatttt agaggagaga aaattacatg aaaaattcag
     1621 gttgtttatg caccgttcca ctcttcgcct ctctttcatt agatgaaaaa aatgagataa
     1681 agtgtttgct tcaccatcag acgtttaaaa aaggagaaat tattttttca ccaagtagtg
     1741 aagaacaact gttgattata tctttagggg ctatgaaaat atatcgaatt agtagaactg
     1801 ggaaggagca attaattcgt atcgtaaaag aaggggatta cgacggtgag aactatctct
     1861 ttggtctacc caatgactgc ctatatggtg aagctgttga agaaaccaaa acttgcactc
     1921 tctataaatc cgactttaat aaagtgatgg cacgctatcc acagctcagt tatcgattgt
     1981 tacaattaaa tgcttgcaaa acagtagctg tggaaaaaca gttgcagctt ctagcgtttg
     2041 atcgtataga ggaccggtta gccttatatc ttaaccaact atctatagca gcaaaaatgg
     2101 gggagcctat tactttacct atctctatga gagagttggc aaactatcta gctaccagtc
     2161 ctgaaactat ttctcgtaaa ttaagagatt ttcaagaaag acaattgatt actagaaaag
     2221 gaaagcagat tacactatta gaagaatttt ggtttatatt taattatata taaagaagaa
     2281 gatattaaaa ttgggattgt cattgcacga ttcaatgatt ttttaactaa aagcttacta
     2341 agtggttgtg aagatggcct tacgcgttca ggcgttttga cagaaaatat cgatgttgct
     2401 tgggtgccgg gggcctatga aatcccactt acagccgcaa cgatggctga aacaggtaaa
     2461 tatgatgcca ttattaccct aggtgcggta atcagaggag ctacaagtca ctttgatgtg
     2521 gtggttaacc aagcgtcaag tggggtatct caagtaggct taacccataa attaccggtc
     2581 atctttggta tcttaaccgt tgaaaacatc gaacaagcca ttgaacgctc tggtactaaa
     2641 gctggtaacc ttgggtttac ttatgcaacg aatactattg aaatgattaa tttattcaaa
     2701 gaaattaaac aacaataagt catcattcta tatgaaaata tctgtaacag catagggcta
     2761 gctttaattt tcaatcatgg taagatggaa gataggaaaa taaagcgcat tcaattaatt
     2821 atcaagcctt tctgatgatt tgcgcaaaaa gcttaaggag acttcatatg acgattcaat
     2881 ttaaacaaac attagcgcca accaaggata attctggaaa accaatcatt ttaagtgacg
     2941 ctaccttcgc tcaacgtaat gagaaagtcc ttcagttaat gcgtgaatat gacttctcat
     3001 cattgatgat ttatgcagat aaagaacacg gtagtaactt tgaatattta actggtttta
     3061 ttccacggtt tgaagaagct atccaagtaa tcaaccaaga cggaacgtcg acactgattt
     3121 taggtaatga gaattacaat aaaaccagta tggcacgtac acaaagtgaa ggtattcttt
     3181 gcccattctt ctctttacca aaccaaccta tggatttcac taaaactttt gctgactatc
     3241 ttaaagacgt taaagtagat gcttcaaaac gaattggttt agtagactgg aagcttttat
     3301 ctaatgatat ggacaacttc cacaaccatc attcaatacc agcatttatt gtagatggta
     3361 tacatgaagt tttcggttct gacaaggtag tgaatgcaac ccaactatac atgcatccaa
     3421 aagaaggtgc tcgtgtgaca aacaacgcag aggaaatcgc tcgttatgaa tatggtgctt
     3481 cccttgcttc tgatgctgtt cttgaagcaa tgaaccagct aaccgttggc caaagtgagt
     3541 tagcagcagg taatcactta aatcgcgatg gtcaatatcc atctgttgtg accattgcag
     3601 cctttggtga tcgctttgag ggtgctaata tttatccaac tgataatacg ctaaatgatg
     3661 gcgataaagt tgcattaact gtagcctata aaggaggctt gtctagccgc tctggctatg
     3721 cagtaaaaga ggaaagtcag ctcgctgaac atgatccagg atacttagaa gaagtggttt
     3781 acccatattt ccaagcctat aaatggtggc tagataattt aaccattggg agaaagggtg
     3841 gcgatttcta ccaagcattc agtgacttct atccgcaagc tacttatggc tggaatttgt
     3901 gcccagggca tttagtagct gacgaagaat ggttaagctc accattttat caagactctg
     3961 atgcaacaat tcaaagtggc tacatcttcc aactagactt tattccttca caagcaggcc
     4021 acaatggtgt ttcagctgaa tcaacgattg cggttgcgga cgcggcacta cgtgaacaga
     4081 ttaaggctga acaacctgaa ttatgggcac gtatctgtaa ccgaaaggat tatctaaaag
     4141 aagcattagg tttagaatta tcagaacatt tactaccctt agcgagtacc tttgcctact
     4201 atagaccttt cttattagac aaaaaaactg ctatctactt tggataaaaa aatgcccctc
     4261 aagattgttt gccaaacttg agggtttttt gtctattttt aagtgaataa tgacggacta
     4321 tccaaacgga atcgccataa agatgttaat atatgtataa tattataaag aaattggagg
     4381 acaatgccct tggacgagca gattgaatta ttatttacca tagatgaagg ctatttgaat
     4441 cccttaaaag tggctttaac atcgattcgt cagaataatc caggccaagc tttccgcatt
     4501 tggttgattc acgaatccat tgcaatgcat acgattcgcg aattgcagaa gctaacggat
     4561 tatctacagt ttggttttga agcgattaaa attgatggta gtcgatggaa tagcgctaaa
     4621 actgaagata gatacccaaa agagatgtat ttccgcctgc ttgcgggtga aatattacct
     4681 aaagaaatga aacgcgtgat ctatttagac ccagatattt tagtggttaa ccctttatta
     4741 gatttgtggc aaacagattt agaaggttat atgctagcag ctgctacaca tgttggattg
     4801 acggacgttt ctacacgagt aaaccaaatg cgacttgacg ttgaccatgc ttattacaat
     4861 tctggtgtga tggtgattga tttagataaa gctagagaaa ttattaaatg gtcagatatt
     4921 gcacaaatga ttgaaaaata taacttgcta ttaatcctac ccgaccagga tatcctcaac
     4981 cgattatatg gtaaatacac taaggaaatt ccagaagaaa tttggaatta tgatacgcgt
     5041 aaatatatgc ggtatttcac caaaagtctc gctcaacatg atattcactg ggtgatggcg
     5101 catactgcta ttctccattt ctgtggtggc ccaaaaccat gggacgataa acatgataat
     5161 cgttttacca gtctttatct tacctatcaa aatcaattaa agcgtattga agatttgatg
     5221 aagtaaagtg aaacaagtgt ttttcattac ctacggtggt ttaatgctta tttattatcg
     5281 ttaaatcatg cttatacact ttacaaaatt tgattttcga aatggccgtc caacgaagtc
     5341 tgcgatctta accacgagtt ccattgacat ggccaaaaag tattatcgag cgatcaaaga
     5401 aatgacgaaa gaacctgact ggttaacaaa agagtttcct aatcagccca ttcgtgaagg
     5461 aagaacgatg gaggatcctg attttccacg aatcgctatt acctattcat tagaagaaaa
     5521 taaccaagat tcaagtgccc aacaagaaga aatgaaaaaa attattgagg aatataatca
     5581 gtattttgat actgcctggt cattagcaga cattgagcgt tataacggag acatcaataa
     5641 ttgtttggca cgaaaaagag cggaattcaa acaatttggg aaacaaatcg atttagtaat
     5701 cgtagtggat cgtctattga caggttttga cgcacctacg attcaaaccc tatttgtgga
     5761 tcgtaatcta gagtatgcag gattgattca agctttttca cgtacgaacc gtacgtatcc
     5821 tgaaaagaca aaaggattga ttgtgacgtt tcgtaagcca gcgacaatgg agaagaatgt
     5881 cgaggatgcg acaaaactgt attcagaagc aaaagaagaa tcgggtttga tttatccaag
     5941 ttatcaggaa tccaaaaaaa gattcaagca agcgtatggt aagttaaagg aggtttcatc
     6001 catgaaatcc attgatgaac atacgccact tgaaatccgt atcgagtacg ttaaagcatt
     6061 tcaagaattg aatcgtgcgt atgaatcgtt agtgacttat gacgattaca atgatgacat
     6121 ggtgcagtct tcaactttga acaatcaaat tcaacttttg gaagaacaaa ttggcgtgta
     6181 cgagaccgtg aaggggtcat taatagagga agaaccagaa aaagagggag aagatttttc
     6241 aacgatcgaa ttttatagtg aaaataccac taagttgtat gatattgatt ctacttatat
     6301 tgatcaatta ctaggaactt atgctgcgaa tagttcagat gcacgagatg aaattgaaaa
     6361 agctttggct aaattgaata aaacagaagg agtcaaagaa gtttaccgac aaattttaaa
     6421 tgcatttgat gtggggactt tagatagcaa tgaagatatt tttgtcatta aacgtcgcta
     6481 ttttacacaa gcaagtgatg aattgattca tgccttttcc aatgagtggt ttgtttctga
     6541 gaaagaatta catgcttcta atctgcaata tatgccaggt gaagatccaa ttcctaatat
     6601 gaaagcaatt atcaacagta aggattacga aggatataaa gccaaacatc caaaagcgaa
     6661 gccatttaaa tacccacaag aaatgaagcg agcctggcgt aaaatgttag atgatgagtt
     6721 gattccactt gagaatgaat tgaggtaaac gagaaaattg aagaaattgt atgataaaca
     6781 tgttaataaa tgatgctcga aataaaattt ggttttattt ttacattgat acggtaaata
     6841 aaatttgaaa gtagaagaaa atatgaacaa caatgaaaca aaaaaaacat atgaactaag
     6901 aataactgct gatgagatta aatcaacaga cccaacattt actatgaaga atctaagctt
     6961 aggggataat tatcaaccaa aagatgctta cttccgtacg gcagaaggcg aaataactag
     7021 aagaaactac ggtaaaaatg aacggaaaat ttcaaatcaa acattaccgc gattagctat
     7081 tcaaatgttt gaacaatcga ttgtttctct accagaaagc gaaagagcaa catttcctgt
     7141 gtgtcgatat cactcaggag atgagataat acgagggatt tttccagatg ttgacagttt
     7201 caaaaaaaaa aggaagtcca ttgaattttt gatttataaa tataatgaag acaagcagat
     7261 agtcatctat tgttggagta ttttttcaac tttattattt gtacaagaat gtttgaaacg
     7321 ttttggaaca tctggggatg aatttattct tgtttataaa gggaaagaga aaaaggaaat
     7381 acgagtagga gagactcaga ctatgaccaa tcatttgctg aaatcatatg ttttacattt
     7441 actcaaatct aaaaacctta ttctacgtgg cgctcctgga acagggaaaa cattccttgc
     7501 aaatgaaatg gcagctgatg tcgtttcaaa tggcagaact acacaaatca atgaattgag
     7561 cgaagatgaa aagagccgaa ttggatttgt ccagtttcat cctagctatg attatactga
     7621 ttttgtcgaa ggtttacgtc caactacatc cgatgatggc gttgttagtt tcaccttgaa
     7681 agatggaagc tttaagagct ttgttgaaaa agcaaagaaa acaaagaaag taaatggaca
     7741 agataatttt gaagaagctt gggctaaatt tttcgatgcg gtaacggaag ctagtataga
     7801 gggagaaggc tacaataaac ttaaaacatt gacaggtaaa ccagtacgca atcttctagc
     7861 ctatgaaaga aatgaaattc aaggtgttta tcctgctaaa accaatactt atctaaacca
     7921 tgaccaaatt tataatgtct atcgtggatt gccaggtacg ccaaaaggtg gatttgatgc
     7981 ttatcgaaaa gcgattgtaa aacatttaaa agaagaattt ggcttaaaaa actacacggc
     8041 gtcaacggta gattctacga ataaccaaaa ctatgtgttt atcattgatg aaattaatcg
     8101 gggcgaaatt tccaaaattt ttggcgaatt atttttctca attgatccag gatatcgtgg
     8161 gcataaagaa ggtgtttaca ctcagtatgc aaacctccat gaaaatccag aagaaaaatt
     8221 ctatattcct aataatgttt atattattgg gacgatgaac gacattgacc gttctgttga
     8281 cacttttgat tttgctatgc gccggcgatt tactttctta gaaataaaag cagaagattc
     8341 agcacaaaat atgctgactc aagagaaaac aaaagaactt atgacgcgtt taaatgaggc
     8401 gatcgttgcc aaagaaaaag gtggattgtc aactgattac caaattggtg ctagttactt
     8461 tttaagtatc gataatggtg aggagacaat ggaacaatta tgggatacta agttatatcc
     8521 attattgaaa gactattttc atggtgaaca taaggcgaaa gaaaaattag aaatactaga
     8581 aaaggtatac tctaatgacg gtgatttaga tgaagctgaa acctctacac gataatcaag
     8641 aaatcaatga ctcagttgtg caacatccaa tcattacgaa cttagttaat cgtgacttaa
     8701 gtagtttaga gaaagaaaat tttattatct ttccgcaaca gttaaatcaa tccaaggatc
     8761 taagtgaaga taacttgata tttcaatcaa gaaatggaaa aatttggact tgtaatgtcg
     8821 taggtatcct tagcgattca acagatgaat taagaattca ttctagattc gctaatcaaa
     8881 tgaagcatga agatttcttt ttacgttata tgcttcagcg tgttttgaat tacaacgtga
     8941 ttaatgatca ttttagtgca tctaaaaaga tgacatacta cgatttgttg gtatttctct
     9001 ttccttatta tctgaatgaa gcaatgcgta aagggacata caaagaatat gtcaaacgag
     9061 aatacaataa tgccaatatc aagggagctg ttgatgttgc aaaacacatc cgttcgaatg
     9121 ttcctttcgt tggaaaagtt gcttatcgaa cacgagagtt tagctataat aatcacctaa
     9181 cccagcttat tcgtcatact attgaaaaaa tccaaaatga gtacgatttc ttacttagtg
     9241 gagatgagga tacgaaagaa aacgtggttc taatcaaaca aacaacacct ggttatgcta
     9301 gattaaatca atttaatatc cttcaagaga atatccttca tccagtcaaa catagttatt
     9361 acgaggagta ttcggctctt caacaaatct gtatccaaat tttatcggaa gaaaaatcag
     9421 gatttggaag tgataaaaat caaatacatg gaatcattat cgatgtgtct tggctatggg
     9481 aagaatacat tggaaaagtc actggttgga aacattatgg cagagataaa gggttagcaa
     9541 caatgcattt atttcaagag cccaaccggt ccccacgata tccagatttt acatttaata
     9601 atataccaat tgatacaaaa tacaaaaaac atcttgatac tagaaatgac tataatcagt
     9661 tagtcacata tattcatata atgaatttag accaagcaga tacaatgaaa ggcggctttc
     9721 tgcaacctac ttccgatccc cttcctgcaa atcatgggta taaaaaaata ggcatattaa
     9781 gcgggctagg aggagaaata ttcacatatc attttttcat tccgcaagct gcgtcaagtt
     9841 atttggaatt tgtagaacag atgagagagg tagaagcagc actacaaact acttttcaat
     9901 aagtaggaat tatagaaatt ttactcaaaa aatcttcatt aaaaaatctg actaaataag
     9961 ttgagaaaac agaatatact tcaatatcca gtcatttata aatatcattc actcatgata
    10021 cccagctccg tatcacaaat aatgatattt tttaagagaa aaaattatcc ctattttaaa
    10081 taatagcaat aggtagtggt agagagaaat ttataaataa ctttgttatt tgctttggta
    10141 gccaagtaag ttgcaaaaga aaaatagata ctgttaaaaa gataatgtag acatagaaac
    10201 aaatacgtaa aattttagtt agaatgatgg caaggagaaa aaacatgata gatgaaaaaa
    10261 agtgtttaaa aaatctattg gaagatatgg atgatattga agaaaaacta tctaaatgga
    10321 ctgggaaaac aaatatattt cagaatccgt ggtgctagtt aattttaata tcctaaagaa
    10381 tatctcaaag ttccttcaaa acgtcgttgt gctcgactta atagaacact gtaagccagt
    10441 aatatgcttt caaatctact ctctagccct ttaacagaac gtgagttgaa attttgacaa
    10501 cctaattttt ctaaggaaga aaaaacagtc tcaactgttt ttctttgttt tccaagtaag
    10561 ctgtggtcta gtttttctga tttatccatg tttttacgtg gcggaacgga tatagtaatg
    10621 cccttaaggg caagcttttc atggatttca tggctaatat atcctttgtc ccctaggata
    10681 ttgggaagat tcgcttcttc cgataaagtc tctaa
//
LOCUS       DAFEPQ010000078        10653 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.080, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000078
VERSION     DAFEPQ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.080"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(81..401)
                     /locus_tag="LP543_002291"
     CDS             complement(81..401)
                     /locus_tag="LP543_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322433.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(424..858)
                     /locus_tag="LP543_002292"
     CDS             complement(424..858)
                     /locus_tag="LP543_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL2322434.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            1193..1831
                     /locus_tag="LP543_002293"
     CDS             1193..1831
                     /locus_tag="LP543_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL2322435.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(2155..2238)
                     /locus_tag="LP543_002294"
     tRNA            complement(2155..2238)
                     /locus_tag="LP543_002294"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2202..2204),aa:Leu,seq:cag)
     gene            2766..3266
                     /locus_tag="LP543_002295"
     CDS             2766..3266
                     /locus_tag="LP543_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2322436.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(3456..3800)
                     /locus_tag="LP543_002296"
     CDS             complement(3456..3800)
                     /locus_tag="LP543_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBL2322437.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(3954..4874)
                     /locus_tag="LP543_002297"
     CDS             complement(3954..4874)
                     /locus_tag="LP543_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL2322438.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            5061..5552
                     /locus_tag="LP543_002298"
     CDS             5061..5552
                     /locus_tag="LP543_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBL2322439.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(5576..7951)
                     /locus_tag="LP543_002299"
     CDS             complement(5576..7951)
                     /locus_tag="LP543_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBL2322440.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            8277..8903
                     /locus_tag="LP543_002300"
     CDS             8277..8903
                     /locus_tag="LP543_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL2322441.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            8926..9630
                     /locus_tag="LP543_002301"
     CDS             8926..9630
                     /locus_tag="LP543_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL2322442.1"
                     /translation="MTETTLYIVRHGKPMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            9822..>10653
                     /locus_tag="LP543_002302"
     CDS             9822..>10653
                     /locus_tag="LP543_002302"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBL2322443.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         3189 a         2087 c         1864 g         3513 t
ORIGIN      
        1 tagtttcatc ccaaataacc ttttttcatt tttactcctc gaactaatca gttttctaaa
       61 ggctgattgg ttcgtctttt ttatttttcc tccatttcat tctgctcttc tgttaattta
      121 tctaatgctt tgattgatgc tgcggctctt gcataatcat agctgtataa ttccataaat
      181 ttatcaggca gaacaaacca cgctggatga taaccgttag ctgtgacagg gaatccttta
      241 tcttgttggt tatctccatc ccatctcaga gcaacacctt tttttccttt ccactttact
      301 aaagcgatcg agtatccctt ctttgtttgt gtgccgtctt caataattgc taaaatttct
      361 attttttctt ttggcgcacg gacttcttga ggacgaacca ttttctattc ctcccttatc
      421 tcattacttc acgaccaaca cactgcaagg ggcatgtgat acgacataag aagcggtcga
      481 tccaactaat gcttgttgta tagcaccttt tcctgtcgct cccataacga tcaaatcaat
      541 attttcttgt ccagggatga cattggcaat caaatttctt ggatctccag tttcaactat
      601 tgcgtggatc tcagcaatgc cgtactcttt ggcatcattg atttttttca gcatggctac
      661 ttctgttctt tgtttttcat ctgcatagat ttttgaaaag gcataggcac tatgggtcag
      721 ctcaactttg ttgatgattg acacgataaa taagtgggct tcattgtcct ttgctaagtc
      781 aagggcttca gcgaacgctt tgatcgattg tcttgatccg tcaactgcta ccatgatgtt
      841 ctggtatctt ttactcattg gaaccgcctc ctttttcttt tattgtaaca tctcaagcaa
      901 aaatacaaaa aatatacgca atatgtaaat aaaatgatga acaaaagcaa tttctttcaa
      961 gttatttata catttctttt ctcatcagaa cacctgttct aatcccatta tttctgcatt
     1021 atgtgaaatt cctcatcatt tttcacatcg agcgaacatc attctgttac aaaaaacgtg
     1081 gatttgtcat aaattgtcat gcttctttta cctatgaaat attttcacgt gttaaagtat
     1141 tcctcgttga acacaacaaa taaaactttt taacgaataa aggagatttt tcatgacatc
     1201 acttaaaact ttactttttg gaacaacttt ggctgccggc gctgcattct tcatgggaac
     1261 gactgctcat gcggacgaag cttataccgt tcaatcaggt gatactctat caacgatttc
     1321 tcaaaaatac gttggtgata actccttgat caatgccatt gcagaatcaa attcaatttc
     1381 tgatattaac ctgatctact caggacaaca attaacaatc ccaacagaag gctctgcaca
     1441 agcagctgct gaacctcaag cagccgttca agaagcacca gtccaagctg aacctgttca
     1501 agctgaacaa cctgttgtac aagaaacggt tcaaacggaa acacaagcag cgcctgttgc
     1561 tgaaacacaa cctgctcctg ctgtaacaga aactgctgct actccagcaa gtacaagctc
     1621 tgcaaaagaa tggatcgcac aaaaagaatc aagcggttct tacacagcaa cgaacggacg
     1681 ttacatcggc cgttaccaat tagattcttc ttacttaaat ggtgactact ctgctgcaaa
     1741 ccaagaaaga gtagcagaac aatatgttac ttcacgttat ggttcttggg aagcggcgaa
     1801 agcattctgg gaagcaaacg gttggtacta agattcatta tttgaacgtt taaacacatt
     1861 caaaatatag taagaggtca tgcgatttat tctttcgcat gacctctttt tgagtttaag
     1921 tttagtcttt attccttaac tatcgtttgc cagtatttct tgtatcagaa tctataaatc
     1981 tactatttga atcaatctat taggttttaa actgtcccac cttcacacaa attttttttg
     2041 atttatttga atctggttcc gttgtttaaa atttttccta tattctttta gataatatgt
     2101 tattttcttt ctcatcgctc caggtgtacc ttctctttat aaaaaaacaa attatgcggc
     2161 taagaagact cgaacttcca cgaggtcacc ctcactagcg cctgaagcta gcgcgtctgc
     2221 caattccgcc atagccgcat cacatctttt agtttaggat tacatgtgtt ttttgtcaag
     2281 aacatatttt tgttcttttt catttctgta cgccctccct tatgccatct acacaaataa
     2341 ttcatccgag caactccatc atctattgag ggttggtgaa aaaatctgtt ttatccatat
     2401 gatcgatttc aatgttaaac gaacgactct gggattagta actattttat ttttttcttc
     2461 atttcttttt tcctcttttt ctcgattcag ctatcttctt tcatttggat attgattatt
     2521 tcttctgttg attactatcc ttctggaatt ctagacgctg aagtaattca cgttttcttt
     2581 cttctagaga tcataagtaa cgaatgaacg aacttcaaat aattttttta ttgtaattca
     2641 tatttgtatt gttcgatgat tcttaatacg atatgacaca ataaataaaa ataggcatgg
     2701 ctatttacaa caatagtgct atagtagaaa acaaatgaat aaggttactt aaagaggtga
     2761 atacgatgaa actgatcaaa ctatgggaag cagatttaaa gaaagcttat cagctacaaa
     2821 ctagctttgg accaaatgag agtggattta tcaacaatgc ttatggctat acatttgctg
     2881 aatttttaga ttatgtaaaa aaatgcgaaa aaacgtcttt aggaatcgat cttcctgaga
     2941 gttacgttcc cgcaaccgtc tttattttag aaagtgatga acatgagtat gtaggaattt
     3001 ttaatttgcg ccataaactg aacgaaacat taagaaacgg cgctggccat attggttacg
     3061 gcatctcccc taattatcgt aaaaaaggat atgccacaca aggattagca ttagtactac
     3121 aagaggcaaa aaaaatcggc attgatgagg cacttttgtc tgtaaataaa aaaaatcttg
     3181 gcagtcttaa agcacaacaa aaaaatgggg ctgcgattta tcgtgaagat gagaaagaat
     3241 actatacgaa aatcaaactg atgtaaaaaa gaaggattcc aaaaggaaag ggatttgaat
     3301 gtcctttatc aatagaacac atttatttaa ttaacttata atatagcaga caaaatttta
     3361 taaacaaaga aaccttttat ctcttttctt ctagaaagga taaaaggctt ttttgttttt
     3421 cctatttgct tctcatagct agtcagccaa gatttctatc gtatccccaa cgcaatccgt
     3481 gcataacggc tcatcttatc tgtcgtccat gctggatacc agactagttt gacttctgct
     3541 ttcgttactt ctggcacttc ttttaatgcg tcatggatag attctgttaa aatatctgct
     3601 aatggacagc ccatagttgt tagagtcatc ttaatgactg tttctccatt ttcaggattg
     3661 aattctatat cataaatcaa gcctaaattg acgatgtcga ttcctaattc aggatcaatc
     3721 accatttcca aggcagcaag aatacgttct ttgattagtt ctatttcttg tccagaccgt
     3781 tcttggtttg cttcgctcat cgtacgacac tccttaacac gatttttatt tatcatactc
     3841 tttttttagc tgcttgtaaa tgagaattta gaagtaagat tctcgtttaa atttataaaa
     3901 atgttcttat tagatgtaat agattaaatt tattctaaaa taaacctgat tcctcatttt
     3961 ttttgtgaac gattttttat ttctggcaat acgccagttg ttcgatacat tttttgttcg
     4021 ttttcatttg caaaccggcc aaatactgaa tgagttggta aaatattgat aaatgcatcg
     4081 tcgtcgattt catgaacgat ctgatcaaga tcatacattt cgtagcgggt gatgaccatc
     4141 atgatcatgt tcccttgttt ttttgaatag ccaccgacag acggcagtaa tgtcattcca
     4201 cgtaccatcc gttttgctag ttcttctgtt acctcttctg gcttgacggt aacgatcatt
     4261 gctgttactt tttgatgact cgtgtggatg gcatcaatta cttgagtcat tgcataaatg
     4321 gaaataatcg tatacaaggc actttcccag ttaaagaaga agcctgcgat gactacaatg
     4381 ataccattta gaatcagcat gtagtttccg actgtttttc cagttgtttg tgaaagtaca
     4441 agagagatga tatccatccc accagttgta aagcccattt ttaaagataa tcctacgcca
     4501 agacctaata aaacaccgcc tactagggca ttcatcaaaa tattatcagt gatctgtccg
     4561 actggaagca gcatcgtcat caccgataca catacaacat tcacaaaact aaggatcgtt
     4621 gctcgttttc ctactttgag aaaccctaat acaaacacag ggatattcaa aagaaaaata
     4681 aatgttcctg tgtctatctt gatatgaaac cattcatata aaagcgagga aatgatttga
     4741 gcgatcccat tcatccctgc agaaaagaca ttggctggaa tcaaaaaata atttaaagcg
     4801 accgcagcgg tcaatccggt tatcaaaata accactattt tctttaatct ttcattttta
     4861 tgatacgcag tcataatttt actcacaagc acctctcccc tactttggta ttccaaatgt
     4921 aagaataaca gtttatctat taaaataaaa tacttcgaac tcaaatgaga agaattttag
     4981 caaacaggta agaatctgcc ctaatcctat gataagatag atgtatggta aatatttata
     5041 tgtatatcag gagggttcct atggcaaaag atgctagctt cgacattgta tcagaagtca
     5101 atacagaaga agtaaaaaat gcgattcagc aaacaacaaa agaattgtcc aatcgctttg
     5161 acttcaagga ttctacagta gaaatcaaat tagagaacag caatctagtt gtattgagcg
     5221 atgatgattt caaaatcgaa cagatcaaag atgtattatt tggtaagctg atcaaaagaa
     5281 atgtgccaat caaaaatatc catttttccg aatcagaaca cgctctaggt ggcaaagctc
     5341 gacaaactgc cgatttggtc aacggaatcg atcgtgaaaa cgcgaagaaa atcactacag
     5401 ctatcaaaaa tgaaaaactg aaagtaaaag cacaaatcca agaagatcag attagagtga
     5461 caggtaagaa ccgtgatgat ctacaagctg tgattgcttt attacgtaaa cttgacttac
     5521 caatcgagtt gcaattcact aactatcgtt aatcaaaaaa cgaggctgac gtctgtcatt
     5581 cctcgttttt ttgatttact gcttcttttg ataaccaaaa acgctgtatt ccttcttcca
     5641 attccccaac acttcgtact tctcttgctg tttgccaatg ttgtgggaat aaacgtcgat
     5701 aagaaaaact agaaaatcgt tgatctgcca atacaacgat tccttgatcc gccatgccac
     5761 gaatcactcg accaccagct tgcaagactt tgttcattcc aggaagctga taagcgtaag
     5821 caaatccaag gtgttccttc tcatcataat atgatttgat cagttcctgc tcatgattca
     5881 tctgcggcaa acctacacct acgatcatac tgccgatcaa tcggtcccct ctcaaatcga
     5941 ttccttctga aaaaatcccg ccaagtacgc aaaatccaat caatgtttca gcgggatttg
     6001 tttgaaattt tgttaaaaat cgttcgcgtt cttcctcatt catgtccgtt tcttggatta
     6061 gcacattgct gtcgggatat tgctgtctga atgcttccac cgcaagatct aaatattgat
     6121 aagaaggaaa gaaaacgaaa taattcccga cctttccatc agcaaaagca tgaatcgtct
     6181 ctactaattt tgccagactg tgttcccttt ttcgataagt cgtctctagg taatttgcga
     6241 tgaaaacttg ctggtttcct tctgggaaag gactcggcag tttgtaagcc agactttttt
     6301 gtccccccag tgtttcctga taataagaga gtggtgaaaa actagcagaa aaaagaatac
     6361 tgctttttcc tttatccaac gactgctcaa gaaacaagct tggatcaata caaaactctt
     6421 ttactatcag atcgcgatac gttttttcga ctgttgtttc gtagtgatca tcataaaatt
     6481 cactgacctt caaaaaatga agcaaatcaa aataataagg aagcaattgt tcttgtgatg
     6541 ggtgctcagg aaattctgcc agccattctt taattttttc gcttaattga taacctgcct
     6601 ttactaaaga ttcagccggt gctttttgat gatgataatc ccaatgatct tcttttgcta
     6661 tttctcggat cgaatcaaat tcttttaata gtttgttgaa acggcgatga agttttttga
     6721 attcctttgg tatagcttcc ttacttcgtt tcgttttttc ataagatagt tctgccgaat
     6781 acatctctcg ggaacgattc accaaattat gtgcttcgtc aatcaaaaac agatagtcct
     6841 cgtttttttc atcttcaaaa aaacgtctta aataaacagt tggatcaaat aaataattgt
     6901 aatccccaat gattacgtca caccagatac tgacatctaa tgatagttcg aaaggacaaa
     6961 gtgtatgctt tcttgcataa gtttcgatga cttcacgagt gatctgattc tcatgattca
     7021 aaagatccca taaaccttca ttaatccgat tgtaataacc attggcatat gggcactgat
     7081 ctggattaca ggtcgtctca tctaaaaagc agatcttgtc tttcgccgtc aaagtgacac
     7141 tttttacttc agctcctgta tcttttaatg ctgtcatagc atcttcagct acttgtctag
     7201 ttattgtttt agccgttaga taaaagatct tttctccctc ttcttcccct acagctttca
     7261 aagcggggaa taacgtagag attgtttttc ctgttccggt aggtgcttct acaaacaatt
     7321 tctgtttcgt acggatcgtt ttataagcag ctaccgctag ttcacgctga cctttcctat
     7381 actcatcatg ggggaatttc aaagccatca aagaggcatt gcgtacagtg cgccagtttt
     7441 cttgaaaaac aagccattct ttataatctt cagtcagctt tttaaaaaaa gtttctaact
     7501 gctctcttgt ctgatgttca acctttctcg tgatcacttc ttctgccgtc tggaagtaag
     7561 tcaattgcag atttatttct tgtaaatcat gttcttgact atagatatag gcatacaccc
     7621 ttgcttgatg aaagaaaagt tccacttgtt ctggttctaa atcttcaaat cgtggttcgg
     7681 aggttttgat ttcatctata taataaatac cgtctctttt gaaaataccg tctgcacggc
     7741 cttcaacgat caggtaatat tcatctaatt gaactgttgt ttttaaaaag acttcttttt
     7801 catagttttc tcccgcatct ttttggagct tgcggtggat tctcgctcct tctaatgcag
     7861 tatggttact ttttttacga ttgtcgatac ttccacgtcg caaaataaat tctactaagt
     7921 gccgcacaga tatttttgac tgtttgatca tttccaccac ctctttctct ctaagattac
     7981 ctgaaaaaac ctagtttagc aaaaagagct ctctttccaa acataaaaaa acaccctgct
     8041 gattaggcag ggatatagga tatagaattt ggatttttgg atgaatctaa tgtaacaagg
     8101 acatattaaa atgctatgaa aacttacttt ccttctcttt agaatatcca aatacttttc
     8161 atatagtctg ataattcaga taagtaaaga gaaataagta cctgtttttg tttcctagca
     8221 atgaatgcgt tatactaatg gatgaaagaa gttttggcta tcaaggagga atgcacatgc
     8281 atatcgaaag attaaaaaca ggaatgattg aagaaaactg ttatttggtc tacaacgaag
     8341 aagcactttt gatcattgac cctggagaag atgcggaacg aatcaaacaa gaaatcaaaa
     8401 aaacaaacca acagccggta gcgattttgc ttacccatac tcactacgat catatcggtg
     8461 cagtggaacc tttacgcgcg tattatcaaa tcccagttta tgtcagccca ctagagcaaa
     8521 aatggctagg tgatccgatt ttgaatcttt ctggactcgg tagacatgat gatattgctg
     8581 atgttattgt ctcgcctgca gaatatgaat ttgaaatgaa aacttatact ttaggaaaca
     8641 tgtcttttcg cgtcgtccct accccagggc attctatagg aagcgtcagc tttatttttg
     8701 atgattttgt cgtttctggc gacgccctgt ttagaggaag tatcggtaga accgatctgt
     8761 atacagggaa tttagaacaa ttacttcata gtattcggac ccagctgttt gtattacctg
     8821 atgaatttgc agtctatcca ggacatggcg atgcaacgac gattgaacag gaaaaaagga
     8881 caaatccttt tttcaatcgt taaaacgact taggaggaga ataaaatgac agaaactaca
     8941 ctttatatcg tacgacacgg aaaaccgatg ttcaatacaa ttgaacgagt acaaggctgg
     9001 tgtgatacac ccctcacaaa gcaaggacaa gaaggcattc attatttagg tcgcgggtta
     9061 aaaaatgtcg acttttcatt tgcttattct agtgacagtg gtcgtgcaat cgaaactgcc
     9121 cgtattattc taggagaaca ttctaaggga aaagaaattc cttattttat tgaaaaaagg
     9181 attagagaat ggtgtttcgg gtctcttgaa ggtggatatg acatggaaat gtggggtgtg
     9241 atccctcgtg tattgaattt taaaacatat gatgaaatgt ttaccacaga agtttctttt
     9301 gaacaaattg ctaatgctgt atacaactta gataccgctg ggtgggcgga aacttatgaa
     9361 acattgcgag accgtgtatg gacaggattt gaagatatcg cccatcactg cgaaaaaaat
     9421 ggtggtggaa atgttttagt cgtatctcat ggtttgacga tcgccttttt gcttaatctg
     9481 atcgatccta aacagcctgt tcgtgctggg cttttaaatg gcagcgtaac aaaggtcact
     9541 tatgcaaacg gtcgattttc tatccacggc atcaatgata cgtcttatat cgataacggg
     9601 aaaacacata gtcgcgactc gtttctataa tggggatatt ttaattttta ccagagaatc
     9661 ataaaaggca aagtctgaac gagggctttg tcttcttttt tctcttcgtg tacttatctg
     9721 ctttatttct caagtttttc ttaatattaa aaaacagatt gggtttctca aaaaataacg
     9781 gtatactata agatgtatct taattgggaa ggaaggaatt tatgtttttt gcaaatatta
     9841 tcaaagcagt gattctcggt attattgaag gaataacgga atggctgccc atcagtagta
     9901 ctggtcattt gatcctagca gatgaattca tcaaactgga tgccagttca caatttatgt
     9961 cgatgttcaa tgtcgttatc caattagggg cgatcttggc tgtcgtcgtt ctttattttc
    10021 acaagttaaa cccattttct ccaagtaaat caacggtaga aaaaaaagat acgtggacac
    10081 tttggtttaa agtgatcgtt gcttgcatac cagccatcat cattggtttg cctttggatg
    10141 attggttaga agcaaatttc cacaagttct taccagttgc cattgttttg atcgtttacg
    10201 gtattgcctt tattattgtc gaaaaacgca acaaaaacaa aacacctcgt tggacttctc
    10261 tgaatgactt tacgtaccaa gcagctcttc ttgttgggtt attccaagtc ttggcacttg
    10321 ttcccggaac atcccgttct ggcgcaacga tcctaggatc tatcttgatt ggtgcttcgc
    10381 gttttgtcgc tacagagttt tcatttttct tagggattcc tgtcatgttt ggcgcaagcg
    10441 gcttgaagat cgtcaaatat ttagctgaag ggaattcatt caaaatggaa gaaactgttg
    10501 ttcttcttgt aggaacattc gtatcttttg tggtttccgt cttagctatc aaattcttac
    10561 taagctattt gaagaaaaac gactttaccg cttttggctg gtatcgtatt atccttggta
    10621 tcattttgat tggttattgg tttattgcaa tgt
//
LOCUS       DAFEPQ010000079        10616 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.081, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000079
VERSION     DAFEPQ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.081"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(390..1337)
                     /locus_tag="LP543_002303"
     CDS             complement(390..1337)
                     /locus_tag="LP543_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBL2322444.1"
                     /translation="MHLEFSDYESLLVKICEDYYLEDINISEISKKYGITRYKALKYI
                     EDAREKQLVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADY
                     IQELIKKANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQK
                     AAERYESSYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDS
                     VEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGI
                     CKSKFKAESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
     gene            1525..2712
                     /locus_tag="LP543_002304"
     CDS             1525..2712
                     /locus_tag="LP543_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBL2322445.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            2693..3997
                     /locus_tag="LP543_002305"
     CDS             2693..3997
                     /locus_tag="LP543_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBL2322446.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IVTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            4023..5036
                     /gene="citC"
                     /locus_tag="LP543_002306"
     CDS             4023..5036
                     /gene="citC"
                     /locus_tag="LP543_002306"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBL2322447.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            5040..5333
                     /gene="citD"
                     /locus_tag="LP543_002307"
     CDS             5040..5333
                     /gene="citD"
                     /locus_tag="LP543_002307"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBL2322448.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            5338..6243
                     /gene="citE"
                     /locus_tag="LP543_002308"
     CDS             5338..6243
                     /gene="citE"
                     /locus_tag="LP543_002308"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBL2322449.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            6236..7795
                     /gene="citF"
                     /locus_tag="LP543_002309"
     CDS             6236..7795
                     /gene="citF"
                     /locus_tag="LP543_002309"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBL2322450.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            7792..8349
                     /gene="citX"
                     /locus_tag="LP543_002310"
     CDS             7792..8349
                     /gene="citX"
                     /locus_tag="LP543_002310"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBL2322451.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            8342..9205
                     /gene="citG"
                     /locus_tag="LP543_002311"
     CDS             8342..9205
                     /gene="citG"
                     /locus_tag="LP543_002311"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBL2322452.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            9439..9848
                     /gene="tnpA"
                     /locus_tag="LP543_002312"
                     /pseudo
     CDS             9439..9848
                     /gene="tnpA"
                     /locus_tag="LP543_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(10485..>10616)
                     /locus_tag="LP543_002313"
     CDS             complement(10485..>10616)
                     /locus_tag="LP543_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL2322453.1"
                     /translation="RRREKIIRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYIKFAK"
BASE COUNT         3729 a         1644 c         2085 g         3158 t
ORIGIN      
        1 cgtggagaaa taataagaac taccagtcag ataaaacaag ataccgaaga ctcactgcgc
       61 aaagggtggg gaatgaataa taaagttaga tagaaccccc tttttatagc ataaaaaaga
      121 tttagaagga tatatgatcc tattgcatga gaagtacaat aaaattaata agaagtaaca
      181 ttaacgaaaa aacgtggaaa ttattcagtg aatacaaaga aattaaatct tgaaaagtaa
      241 tggagtatgg cttggaaaag ttcaaaaaat atcaagacaa gcttctcaaa aaaagggatt
      301 ttgatatcca ataaaaagag tgaatcattg tactgccctc caaaaaagta gtaccaaaaa
      361 tctaaatttt ttggaggatt ttacatgact cactcttttt ttatcaaaga aagtattttc
      421 caagcagttt tttcatccaa aaataaatct gtaaaaaaat taccattcaa ggcgcctaaa
      481 caactctccg ctttgaactt acttttacaa ataccaattc gttttggaat atcgaaaatt
      541 tcttttaaag aaagaccaaa agtcttatcc tcagatgctt tgatgaattc accattgata
      601 ttgaagggtc ttccgtaaag aaatccaaca gtatccgtca tgcttggaaa taggatttta
      661 cgatgcttgt tccagggttc gacagaatca atagaggata aggtgccaat tccagaaatt
      721 aacatatctg catgcgaagc ggttgctaag gtattagcta aagaaggttc tagagccatt
      781 agttcacgtg cagtttcatt gatgacatat aaaggaatac tgagagtcaa ataagaagac
      841 tcataacgtt cagcagcttt ttgaactaat cgtgtggagc cagctaagga attatatttt
      901 ccgatttcgc caatgaattg agtaaaaatt aattcttcac gagcacccat tttaaactga
      961 tcaatcactt tgtaaacggt atctccccaa gatagtgcaa ccacattggc tttcttaatt
     1021 aattcttgaa tgtaatcggc agcaaatttt gaaaataata aatcgtgttg tgataagtct
     1081 tcactacttt ttaatatata gatattcgtt ttaaagtaat ttttgaagat ttcttctagc
     1141 tcataattcc tagcatacgg agaattaatt gaaatactaa ctaattgttt ttcacgtgca
     1201 tcttcaatat attttaaagc tttatatctg gtgatcccat attttttaga aatttcacta
     1261 atattgatat cctcgagata atagtcttca caaattttta caaggagtga ttcatagtca
     1321 gagaattcca gatgcatagt atagctccta actttttcaa atagatattt tttctcatta
     1381 taccaaaaaa attgaattta gtcttttttt aaatttttaa aaataagcat cattttttta
     1441 aaatttaaaa tgaaagggtt tttttatttt aaaaaaagtg ttaaagtggt tttgtaatta
     1501 aaaaatgtaa aaaggagtga aggtatgctt gatgaagttt tagaaatcca taaaaaaaat
     1561 gtaggtgttt tacggatgga aacaacagaa gccgtgcaag atgatcaaga tttagcaaaa
     1621 atgtacaccc caggtgtagc agaactaagt ttaatgattg agaaaaatca tgctttggca
     1681 agagaattaa caattagtgg taaattagtg gccgttatta cagacgggtc ggcggtatta
     1741 ggtcttggaa atgtaggaaa ccaagctgga ttaccaatcg tcgaagggaa agcgctgctt
     1801 tataaaaatt tagcaggtgt caatgcgatt ccattagcca tcgaacaaaa atcagtagac
     1861 gagattgtac aaacaattgt caatttgcaa aatagttttg cagggatcca tttagaagat
     1921 attgcagcac cgaagtgttt tgagattgaa gaaaaattac aagaaaaact ttctattcct
     1981 gtgtatcatg acgatcaaga aggaacagcg atagttgttt tagctggatt gatcaatgct
     2041 gctaagatcc aaaggaaaac attaactgaa cttcgagttt tgatcaacgg catgggagca
     2101 tctggggttg caacagctag actattaatc gctgctggta tcaaaaatct aacattggtg
     2161 gataaacaag ggagattaaa gaagcaagat agtacattaa ataaatatca aagagagttg
     2221 ttggatcata tacatgatac aaataataat gcaagcgttt tggaagatgc aatcgttgga
     2281 caagatgttt ttatcggtct ttcaatagga aatgttttaa cgcctgaaat gattggaaaa
     2341 atgaatgata atccgattat ttttgcttta gccaatccag taccagagat cacacccgat
     2401 ttagcaaaag aatctggtgc aaagctaatc gctacaggtt cttcgaaata tcctaaccaa
     2461 gtaaacaata ttttagtatt tcccggattg ttcaagggac tattactatc gaaagcgaat
     2521 aaagtcgatc tttcattaca acttgaagta gccaaacaat tagctgctaa ggtcgcagag
     2581 cctacggtag aaaaattcat ccctaatgtt tttgatgaag gagtcgcaaa cgtcgtttca
     2641 caggcagttt tggattattt acaaaaacta aaagaaaacg aggataatat acatgctgaa
     2701 aacattaaat agaattaaaa ttggttttat tcctttatct ctttatatca ttttggtact
     2761 cgcgtatctt gcaatgacga taagcaatat cgttccagaa aatgatatgt tagtttcttt
     2821 gggtattgtg actgtttttg cttatttatt agaagaaatt ggtaaaacta ttcctatttt
     2881 aaaagattta ggtggtaaag tattagttgt tacatttttg cctgcatttc tagtctataa
     2941 acattggcta cctcaaagta ctgtaactgt ggtttctgat tttatgaata ataacaattt
     3001 cttgatgttc tttatcacac tactagttgt cggcagtatt gcttcaatga atcggcaaac
     3061 attggttaaa gcaacttcac gtattatcat tattttaatt ttatgcgatg tgattggttc
     3121 tttgatagga acaggtgtgg gaatactcct tgggctatct gcttggaatg cgtatttctt
     3181 tatcgttgcg ccgattatgg ctggtggtgt tggggaagga gcgatgccat tatcgattgg
     3241 ttatgcagca atattatctg gtatgggaca aaatgatatc tttgggaaga ttctaccgtg
     3301 tgtgatgttg ggaagtttag tcgcagttct actgtctggt atgctcaaaa aaattggaga
     3361 gaaataccca caattaaatg gtcagggtaa tttgatcgat ggggatgatg aacatttaga
     3421 gaacttgaag ggttcagcta aaccagtcaa tattgaaaaa acattgttag caggcgtggt
     3481 agcaataacg atttattttg ttgctgtcta tgttgagcac ttattaaaat cactccttgg
     3541 cttttctatt ccttcacctg tgatcttatt agtctctctt atgggggcta aaatgcttgg
     3601 ttttatccct aaaaatatcg aagaaggtgg aaattcgttg tatggattca ctgttcgtgg
     3661 gatcacacct cccttacttt ttggcgtagg ggttgcaaaa acagattggt tagagctgat
     3721 cgaagtattt aaaaatcctg caacgatagt aacaatcatt gtgacggtag tagctattgt
     3781 cgctacagct tttatcgccg caaaattttt aaaaatgtac ccagttgata ctgccatcgc
     3841 agtttcatgt tgtagcggac aaggcggtac aggtgcttta gctatcttag ctgcgggtga
     3901 tcgcatggaa ttaatgccat ttgcacaagt tgctgttcga ctagggggtg ctatgaccgt
     3961 aacgttcgct atctttttaa tgggtctgct atcataagaa cataaagaaa ggacgtgacc
     4021 atgtgtttga tcaaacagat atccaacgat tatttttaaa aaataactat atcaaagacc
     4081 aatgggaaaa tcttttgctt gattcaggaa tacaaaagaa gcaaatcgaa gattttcaac
     4141 atttagatca aacattgggt atataccata aagaaaagtt agtaggaaca gtatcttatc
     4201 aaaacaacgt gattaaatat atcgccgtgt ctgaaaaata taaagatggt ggacatacat
     4261 ttaatgtact agtctctgca ataattcagg caatgagtca acaagagatt tttcattata
     4321 ttgttttcac gaaaccgcgc tatcaaaaaa gttttaactt tttaggtttt gaaacaatcg
     4381 tatctacccc aaaaatagct gtattagaaa tgggagatag ttctataaaa cagtacctag
     4441 agaggattcc taaagtgaat ggtgaaaaaa aagcagcaat tgtcatgaat gcgaacccat
     4501 ttacagaagg gcattattat ttagtaaaac aagctgccaa taacaacgat tgggtatatg
     4561 tatttgtact cgaaaaagaa caaaatcttt tttcgacgaa ggaccgactc aaattagttc
     4621 aacaaggatt acaaaaactg aaaaatgtca ttgttctatc aggtggcgag tatattatta
     4681 gccctgcaac gtttcccacc tattttttac gagaagatga tgaaatagca aaggagcaaa
     4741 tggtcgtaga tgctacttta ttcaaagaaa gaattgccac ttatttagat atctctgcta
     4801 gatatgtggg agaagaacca ctttccccaa tgactcgttc atataacgac atactggaaa
     4861 cgattttacc ccctgaaata aaagttcatg tccttgcaag gaagaaaacg gaacaacacc
     4921 aagttatttc tgcttctcaa gttcgtaaag cgtatcttgc tgggcaattg gaaaaaataa
     4981 aatatatggt tccagaaaca acctatcaat acttaaaaaa taaaagggag cgttaagtaa
     5041 tggaaataaa taaatcagcg attgctggaa cattagagtc aagtgatgtc caaatcatga
     5101 tttcaccgaa tacagaagga atagaaatcg atttagacag tcaagtgatt gagcaatttg
     5161 gaaaacaaat caaaaaagtt atctatgaca cactagcagt ttatcagatt aaaaacgtaa
     5221 aactcaaggt agtagataaa ggggcattag attgcgttat tgttgcacga ttaaaaacag
     5281 cgatccatcg ttccttaggt atccaacacc aagaaacacc gtgggaggtt taattagatg
     5341 atgaaagaac gtttgagaag aacaatgatg tttgttccag gaaataatcc agcgatgatc
     5401 aaagatgcag gtatttatgg tgctgactcg attatgttcg acctggaaga tgcagtgtct
     5461 atggcagaaa aagacagcgc aagaatgctt gtggctgagg cattgaaaac agtggattat
     5521 ggtgacacag aattaattgt tcgaataaat gacctgcact cagaatttgg acgtgcagac
     5581 atttttgcaa tggttaaagc cggcgcgcat gtattacgtt tacctaaaac agaaacctct
     5641 gaagatataa cagaagtaga aaaagtgatt atagaagcag aaaactatca taatgttccg
     5701 atagggcgaa cattgatgat ggcagccatt gaaggtgctt ctggtgtatt gaacgcacct
     5761 gcgatcgcca aagcatctga tcgattgatt ggaatcgcca ttggggcaga agactatgtg
     5821 acaaatatga aaaccaaacg atatccagat ggaaacgaat tgttttttgc tcgcagtatg
     5881 atcgttcatg ctgcacgtgc agcaggcatt tccgcatttg acactgttta ctctgatgtg
     5941 gatgatatgg aaggattttt aaacgaagta cgtttaatcc atcaattagg ttttgatgga
     6001 aaatcttgta tcaatccacg gcaaattcca gtagtcaata ctgtttatca gccgtcaatc
     6061 aaagaaatca aacgatcact agacatcatc tcggcaatcg aagaagcaaa agaaaaaggt
     6121 tctggcgtaa tttctttaaa tggaaaaatg atcgataaac caattgtaga acgtgcacaa
     6181 cggacattga tgttagcaaa agcatcacga ttgattgatg aggagggaaa tatccatgca
     6241 taataaacaa agacgtagca ttccatcaag tgttttaaca aagtataaaa aagaattcca
     6301 aagtgtttca ttatcgccac aaacgattca acgttgtgca ccaaaagtaa cgactgtaac
     6361 taatcagaca aataaattga tgcactcgat caaagaagct atagaaaaat cgggcttaaa
     6421 agatggaatg acgatttcat ttcatcatca tttgagagaa ggtgattttg tttttaacca
     6481 agtgatggcc gtgatccgtg aaatgggcat caaagaattg accttagcac caagttcttt
     6541 aacgaacact atgaatgaga tggttattga atgcatcaag gacggtacag taactcatat
     6601 cacaagcagt gggatgcgcg gcagcttagg tgaatttatt tctcatggag gaatggacaa
     6661 tcctgtgatt ttacgttctc atggtaatcg tccacgtgca attgaaagtg gtgaattgaa
     6721 aatcgatgtt gcatttctag gagtaccaac atctgatgat tatggaaatg ctaatgcgac
     6781 tgttggaaaa tccgtttttg gttcattagg gtatgcaaaa gtagatgcac aatatgcaga
     6841 taaagtcatt cttctaactg atcatttagt tccttaccct aatacgccca ttagtattcc
     6901 gcaaacacaa gtcgactatg tcgtggaagt agaagcaatt ggtgatccga ataaaatcgg
     6961 tgctggtgct acacggttta ctaaaaaccc aaaagaatta aaaattgctg aaatggtcaa
     7021 agatgtcatt gttcactcac cttatttcaa agatggcttt tcttttcaaa caggaacggg
     7081 tggtgcagct ttagcagtaa cgcgttactt agctagtgaa atggaagaaa aacagatcaa
     7141 agcttctttt gcattaggtg gcatcactaa accaacggtt gatttattgg aaaaaggtct
     7201 tgttgaaaaa gtgttagatg tccaagactt tgacaaagga gcagctgaag gaatggcgcg
     7261 gcttgagaac caacaggaga tcgatgcttc cttttatgct gatccaagta ataaaggagc
     7321 ggttgttaac caattagatg tcgctatttt atctgcatta gaaattgaca caaacttcaa
     7381 tgtaaatgtt atgactggtt cagatggtgt gctaagaggt gcgattggtg gacatcaaga
     7441 tgcagcaacg gctaaactta ctatcatctc agcgccgtta gtacgtggcc gaatacctac
     7501 agtagtaaat gatgttacta cattgatcac accagggaaa agtatcgatg tattagtcac
     7561 agaagtaggg gttgctatca atccccaacg taaagatcta atagctattt ttgaaagaat
     7621 tccacaaatt cctgttttta caatagaaga attacagcaa aaagcagaaa aaatagtagg
     7681 catcccggaa cctttgcaat tcacagatcg gatagttgcc tatgtagaat accgagatgg
     7741 ttcaatttta gatgtcgttc gtcaggtgaa agaatttgaa gaagagagat catgatgttt
     7801 gatactggag aaattcaaga aatcgaggat attttgatcc ataaagaaca acgtgctaac
     7861 ttgcaaagca acctgctttc caagtattca aatagttcat tgatggtctt tacattgaat
     7921 attcctgggc cgatcaaaaa taatccaatg attgagcagg tatttcgtca gggacagcag
     7981 tcaattcagc aaatgttttt aaaaaaaaag gtggaaatga cgtacacaaa agaaatcaat
     8041 ttgccaagtg gattggactg gtacgtaata gtaacagaag cacctttgaa tctcaaaaaa
     8101 tggttggtca cgttagaaga gcaagacgca ttaggtagac tttttgattt agatgtcttg
     8161 ttcaaaaaag gagagcagat aacaattgtc agtcgccaag ctcttaatca tggggaacgt
     8221 ccttgtttta tttgtgggaa atcagcaaag gtatgtgcac gtaatcgtac acatactgtg
     8281 aaagaaatgc aaaagaaaat cgaacaaata gtagccgatt ccttaaaagg agcattgaaa
     8341 catgcatgat aatattcaaa aaatagcaca agaagcacaa ttagcgatgc tttatgaagt
     8401 tacatgtatc aataaaccag gattggttga tccagtagac cccgggtctc atcaagatat
     8461 ggacattttt acttttctac aaagtagtgt agttttgact ccctattttg aagaatttat
     8521 ccaaacagga ttggaccgac aacatcaacc aattgaagaa acctttatgg cgattcgaca
     8581 gattggttta gaggctgaaa ctgcgatgtt ctcagtgaca aatggtataa atacacataa
     8641 aggtgctatc ttttcattgg ggatttttct tgctatttgt ggccggttaa ctatttggaa
     8701 agttccttgt aaaaaaagtt tctttcaaaa agaaattcaa aaaatgacga aaaatctact
     8761 taacgatttt gggaagattg atcaaacaaa accaaaagca tggacttggg gagaatatct
     8821 atttgttaac catggattga cagggatacg aggggaagcc aaaagaggtt atccttgtgt
     8881 ttttgaccga ggattgcctt attatcaagc atatcaaggg tcgcaacaag ataagatgat
     8941 agacacttta ctttttctat caatggaagt agaagatacc aatttgatca aacgaagtaa
     9001 taatagacat gtttttgaag agtatcaatt acttattcgt ccttattttg aattaggggg
     9061 tgtaaaaacc atacaaggta aaaaatattt ggattaccta aatcagcaat ttaaaaagaa
     9121 aaattggagt attggtggaa gtgcagactt attgatcctt acaatctttt tagataaaat
     9181 catggacaaa ggatggttat gttgatacta taatacatta ttttgtatta gtaagctaat
     9241 aactaaaaaa tggttcaata caaatatttt ataaaaaatg gtagtgccta tcctttcaga
     9301 ttgtagacaa agaaaaattt ctttgtctac aatttttttg ttaatgactt cagtcagttt
     9361 cgtgcgcaaa gcgtgcgaaa caagaaaacc atgcgtttct acacgtggaa gaactagcat
     9421 atcgaaagga gttttttcat gtcaaatgac gataaaagtt tagcccatac tcgttggaat
     9481 tttaagtatc atattgtatt tacaccaaaa tatcgacgaa aagtaatcta taggcaatta
     9541 cgaaaagata ttggaaaaat acttaggaag ttgtgcaaaa taaaaaacgt ggaaattata
     9601 gaagcacatg caacgccaga tcatattcac atgcttgttc aagtaccatc aaaaataagt
     9661 atatccagtt ttatagttat ctgaaagatc gtagtgctgt gctaatccac gagcagcacg
     9721 taaatcacaa atatagatac ggaaataaat cattttggtc ggaggaatat tatgtgagta
     9781 cagtcgaatt gaatcaaaag acaattgaaa aatacatccg tgaacaagaa gctgatgatc
     9841 gcatttgaga tagtataagt aaaagagagt agatgttgca ataacaaaag ggtaaaaatg
     9901 gactacctcc agtaaaatac cgaaaaatag tttaactttt ggagataagt ccatttaagg
     9961 gtctatagaa tgacaatttt tttaggggat tttactatta ttacttcaag acccgcttgg
    10021 attcgcaaag tgagtgaact cttaaaacta cctgatagta ggttgtttta agagttatct
    10081 gaaagtaaaa attgcattta tatttttgat aaatacgtaa atttatatat acgtatttat
    10141 caaagtttta ggccaaagga atattgtgta aatacactag gattgaatga atagaccaga
    10201 agaaattgcg tccaagaaca aaagaagcat gatagtgctc tatataaatt gggcattcaa
    10261 gaatctgaaa tttaaaggta gaattttagt tagactttta gattttaaac acaaaaataa
    10321 ccatacaagc aacaagaagc aaagtgctat attataaatt gtccgtccgt ttacttacat
    10381 aagatcattg gggctatttt taaaagagaa taatagataa taaatgaaaa aaaagtcaag
    10441 tccacaatcc tgtgtaaaat actatacaat gctctcacta ttttttactt ggcaaattta
    10501 atatattttc ttgatgaaga aagccactca tcgtgttgat caataaggac tgctccaatt
    10561 aaacgattgg ctgaaacgca gtttggaaag atacgaataa ttttttctct tctgcg
//
LOCUS       DAFEPQ010000080        10614 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.082, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000080
VERSION     DAFEPQ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.082"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            3..323
                     /locus_tag="LP543_002314"
     CDS             3..323
                     /locus_tag="LP543_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322454.1"
                     /translation="MHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLVHLPL
                     FFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNYHGTY
                     KEDE"
     gene            324..917
                     /locus_tag="LP543_002315"
     CDS             324..917
                     /locus_tag="LP543_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322455.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            939..2963
                     /locus_tag="LP543_002316"
     CDS             939..2963
                     /locus_tag="LP543_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBL2322456.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            2963..4162
                     /locus_tag="LP543_002317"
     CDS             2963..4162
                     /locus_tag="LP543_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBL2322457.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            4178..4822
                     /locus_tag="LP543_002318"
     CDS             4178..4822
                     /locus_tag="LP543_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322458.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            4833..5639
                     /locus_tag="LP543_002319"
     CDS             4833..5639
                     /locus_tag="LP543_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322459.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            5662..5891
                     /locus_tag="LP543_002320"
                     /pseudo
     CDS             5662..5891
                     /locus_tag="LP543_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5912..6985
                     /locus_tag="LP543_002321"
     CDS             5912..6985
                     /locus_tag="LP543_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HBL2322460.1"
                     /translation="MKKTYQKFLWISYIVLFIVGFLVSVSVIVGTLFLLFSDEDLVLK
                     GLMSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIEYTWLPLKRNSKR
                     KEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQIAASNIFTAVMTL
                     MCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIK
                     NFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVD
                     FGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYNFYKVEASLRYGI
                     GKLRLDGSNIQQNKPYVLGKVVI"
     gene            7029..7298
                     /locus_tag="LP543_002322"
     CDS             7029..7298
                     /locus_tag="LP543_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322461.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            7574..7849
                     /locus_tag="LP543_002323"
     CDS             7574..7849
                     /locus_tag="LP543_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322462.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHELMDHRKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIE"
     gene            7891..8313
                     /locus_tag="LP543_002324"
     CDS             7891..8313
                     /locus_tag="LP543_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322463.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            8333..9925
                     /locus_tag="LP543_002325"
     CDS             8333..9925
                     /locus_tag="LP543_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322464.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            9946..10281
                     /locus_tag="LP543_002326"
     CDS             9946..10281
                     /locus_tag="LP543_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322465.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            10427..>10614
                     /locus_tag="LP543_002327"
     CDS             10427..>10614
                     /locus_tag="LP543_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322466.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         3903 a         1627 c         2225 g         2859 t
ORIGIN      
        1 acattcatat gaaaaatcct ccaagattta gtggggaatg gaagtttttc gcctttttct
       61 ttttagatct attaacgctt ggtggctgct ttttttgggc gaaaatagtc aataatcttg
      121 tccacttacc actctttttt gcactcttga attatggatt atggcttgcg atagcgttag
      181 tgattgtttg gcgaccctat aataaccctg gtcaacgcca aatttttgcg attatcgaat
      241 ctattttctt tacggataag aaccactatc atgcgttaga tcctaaccga aattatcatg
      301 gtacgtataa ggaggatgaa taaatgcgag tcaaaggaca aaattcagtt gataagaaat
      361 ccattttgca gtttggaaaa ccagaagagc gctctatctt ggacttattt ccatataaat
      421 cctgtacaga agagggagtc ttaattacta gagaagatca ttttcagcgt tattatcgtg
      481 tcgtttccac tgacgtggag ggattaaatg aacaagagaa actagaacga atgaatcagt
      541 tgaccaccgt catgcgtacg tatgtgccaa caattaagtt agttagtttg accacagaga
      601 cggatctaac ggaacaaatc gtgcaaaagc ggcagttatt agataaaaat cgtatggaac
      661 agtcactagg aagaaatcta cagcgattaa gaaaatatga aaagaaatta gtagaggaga
      721 tccaggaatt aaaacgggca gaacaagaac gtcctgactt aagcttcttt ttcgttattg
      781 aaggaaagac aatcaaggat ttacaaacta gaaatcgtca gttaatgcga tctagtggca
      841 ttttagggtt aaaaccactc tacaaaaaag aattgattaa gattctttat cgaatgaaca
      901 acatgaatga cgaataaaaa agatggagga aatgattcat gctagatgtg ataaaaacag
      961 ctttttcaac gcaaagtaat caaaaggtga ttgatccatt aaaacaactt ggtaatcgtg
     1021 aaatagagat tttaaaatca aaaggttatg atttagattt cttaaaacaa gtgatgccac
     1081 aaggtgggtt gaattttgat gaagaatatg tgacctatgg aaatggttat tcacgatgtg
     1141 tgacagttta tcattacaca aaaaatccta ctcttttttg gttggccaat ctcatgaaca
     1201 atcctcatac agtggccaca ctagatgtta gcacagatga aaagcaaaaa gtgattgatc
     1261 aaatcaaccg cgcattagat gaattaaatg atcgatcaga taatgaacga aaagcgactg
     1321 accgaaataa aagtgcggaa gaatacctgg aactagcgga atatgcccga aaattgactt
     1381 ctaatgggga aatttctaaa caagtcaaag tgagagtaca tgtttacgga aatacgatcc
     1441 aagaagtgga tgaacgggcg caggaactga tcgaaaatct aaaagggatg gattatcgtg
     1501 cggtgactta tctgcataaa accaaacgag attggcaatc gatgtttacc atgttcgatg
     1561 aacaagctga atggtttgga aatatcaaag ggcaatcact tccatcgtta aacattggcg
     1621 gaggattccc gttccaccac caaacgctaa aagatccagg aggcacacaa tatggggaaa
     1681 ccatgacagg cggcccattt gtctggaatg ccacgtataa aaatagtgta cgtttatcta
     1741 ccaatacctt gcttctagga atgatgggag ctggtaaatc gaccgttctt aaaatgatcg
     1801 cggaagccca tttagctgca ggagacttta tttggggctt tgaaaaggga aaagacttta
     1861 ttcctttctt aaaggaatat aacggtatta tggtgcgttt agacggatct gacgggatga
     1921 tcaatccttt agagatcttt gccacacgga cgtatgatga agcatccagt ttatatgacg
     1981 atggttcagt caaagatttg aagatcaatg aagcagcgtc ctatcaaacg cacttagaca
     2041 aagtagtcta tcaggtacag ttagtgagtc ctcagctaaa aggaacgatg aaagcagaat
     2101 tcaagaccta cttgaatcgt ttttatgaag agtatggaac agtaccacgc ggttttactt
     2161 cctcaaattc gcgttcaaat actgatacgc aagtcacagg gaaagatcca gaagcttatc
     2221 cgacgttcaa agaattttta gcctttttag gacaactgga actaccaggg gcttcccaag
     2281 agaaaaagaa tcggaaagaa gaaatggagt ctatcgtgga atccttatgt gagacttatg
     2341 gcatgatttt tgatggtcat agtacgattc gacaccttga ccaacaacag ttggtctttt
     2401 ttgacattga ctctattaca ggcctagatc gaggggtaca acaatgtcag atgtacatgg
     2461 ccatgacgtt gatttggaat caggcattga ttcaaggacg aagacaacga cgcttaatta
     2521 aagaaaaaca aatggaagaa caagacttca aacgcttctt ggcgatgatt gatgagtgcc
     2581 ataatgcgat taatccagat ttcattgaaa cggtaaatta tgtcacaaac ttccaacgag
     2641 aaatgcgtaa agtagaagca atgactgttt tagctacaca atccccacaa gaaatggtgc
     2701 cagaaaatat gtcttctgat aacttctcga aactgaagaa agtctttgaa ttttcttcta
     2761 cgaagatctt tatgcgtatg gatgaatcta ttttacgtca tatagaagga ttagttggta
     2821 aatcaatgac gaactcagaa ttagaatcca ttcctcaaca aaatcaagga gacgctgtga
     2881 tcaattttgg ctcaaaagaa accattcgtg ttcattttag tccgaacaaa cgtcaattaa
     2941 aattgtttga tggaggaaaa taatggaaaa ccaaaatgaa agtttaatca aacagtatgt
     3001 taagcgtcgt gcaaagcggc gcttattttt gtgggtgttt ggtacttctg caggtttgat
     3061 tactatattg atcactgtgt ttgtgaccct ttttctaata cttgcggcag gttctattga
     3121 taattctgat tcaagtagtg gtggcgaagc ttttacagga gaatattcgg aaggcttacc
     3181 catttataaa gaaatcaaag gaagaggccc tttttctgat gagattgcgc aatatgctgt
     3241 aggtgcagca gtgaagtata aattattacc aagtgtgatc ctttcgcaat acggttatga
     3301 atcagcgttt ggtacgtcgg cgtccgcaag aaatgatctc aattattttg ggattacgtg
     3361 gtttgatggg tgtctgtttc ctaaaggaac tgcgcgtgga attggtggca ttgaaggtgg
     3421 ctggtacatg aagtttccca actcaaaagc tgccttctcc tattacggct ttatggtagc
     3481 cacacaatca aattttaatg cttgtgtcgg aaataaaagc cctggcgcaa gcttattgat
     3541 cttaggacga ggagggtatg ccgccgcagg aattactgaa gacagtgcct attataaaaa
     3601 ttgtatgtct attattaata caaataaatt gatggagtac gatgagtttg cgattaaaca
     3661 ctggagagaa ggcggcggaa ctggtggtac gattacagga agctggggca atccattccc
     3721 aggaagtagc ttagataaaa attcgttcag tggaggacaa ctttttggga aaaatccagg
     3781 aggagaattc cgacctaacg ggttccatga tggactagac tttggttcag tcgatcatcc
     3841 aggaagtgag attcatgcgg ttcatggcgg aaaagtcgtg tacgttggca atcctggtat
     3901 ttcaggtttg ggtgcgtgtg tgatcgtgat taattacgat ggcttgaata tggtgtatca
     3961 agaatttgca aatagtacag gaaattctcg agtgaaagta ggcgatcaag tgaaagttgg
     4021 tcaagtaatt gggatacgag atacagcaca cttgcattta ggatttactc gaatggattg
     4081 gcgtcaagca caaggacatg cattcattga tgatggtaca tggatcgatc cgttaccatt
     4141 tttgaatagc agcaaaaaat agatcaggag aaaaaatatg gaaaataaaa ccttaaaaat
     4201 cgtcttaaca gctgcaatta gcgttagttt aatcggcaat attgcgttgt tttggcagct
     4261 taaagaagaa caagagacaa atacctataa aattgaagaa gtggctaaaa acgcgtctga
     4321 taagctcaaa aaaaattctt ctgaagcaca ggcgaagatt gacaagttaa atcaaaccat
     4381 caatgagaaa aaccaagaaa tcgatcagct gaagcaacaa gatggcacaa aagcaaaaga
     4441 ggaagtaact gaagcacaaa gaaagacggc agaagagttc ggtcaattgg ccattgataa
     4501 atcattaagc agaaatgaat taacggaaaa actaaaagat gtcgcaaccc aagaagtgat
     4561 cgacaaatta agtcctgtag acgatgatca ccaacatgat gattacggaa gttactcttt
     4621 tacattaggt gacgtgcaaa cttacgctca aacaagcagt gtaaaagagg aacaaaattt
     4681 tgtcgtattt gttgattata caatcggcaa tcctcaattc aaagatgtca aaccacaaaa
     4741 aataaaaggt ggattaactg tcactgaaaa acaagtagat ggtcaatgga aagtcacaga
     4801 ctttagttat ttcactcgtt aggaggaaga aaatgagtaa gaaaaaagga gcaagacaac
     4861 gttttaaaaa acgaaaaagt tatcttagat ggctggggat ctttatgatt ctcggctttt
     4921 tgattcttgc tagtccgatt tacacacata aaaaggtaga cataccagat gaagtaagca
     4981 cgaacgcatt tttaaacacg aaggaactga cgatggtttc aaaagaaaaa ggagcgaacc
     5041 agcaattagt ttttgaattt gcgatcgacg atgaagatca gagcttatta aaagaactag
     5101 ctaatcttaa ttatcgtgta gaagtaaaga cagctaaagg ggattatcaa gcaataaaag
     5161 ccaaagtgat caaggtgtct gatgattatt ttgtagtgaa gttagcacat gtaccagaag
     5221 aatatattgc tatgcgatta actattattc ctgaaaaaat tgatccaaag gtggatatgc
     5281 aagaaccgca agatctgatt ttttatattc acgaagataa ggtcaaagat cacgtgaaag
     5341 acgaagatta tgaacaacat gcgatcaatt acaaagtgaa aggatacaag aaagaacaaa
     5401 aagcagcaca aaaacaaatt gaatccttcc aagcaaccat tgatttgaat gaagaattag
     5461 tgaagaaact aaaagatcaa ttgccttatc aagtcgcaga agatcaggaa aatactgaaa
     5521 ataagattaa tagttaccag caagaaattg aaacatcgaa aacgcaaatg aaagatcaag
     5581 aagagagcat tcaaaaactt caagaaaaaa ttgatcgtat gacaaaagaa aatatttaga
     5641 agaataggtg gagtaaaaga aatgaagcga gcgaacttgg tcggcaaaga tactttcgct
     5701 gagcagaaag tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcaa
     5761 gaagtgaatt atctaacaca acaatatctg agttgaagaa tggtagaaag agtttagata
     5821 ccttaaaatt cagatcattt gaagctctat atatagctgc taaaaaatat tttacagctg
     5881 ataataaata aaaaattaag gtgggttcta tgtgaaaaaa acgtatcaaa agtttttatg
     5941 gattagctat atcgtcctat ttatcgttgg gtttctcgtc agtgtgtccg tgatcgtcgg
     6001 aacgcttttt cttttgttct ctgacgaaga tctggtactt aaggggttaa tgagtacgtt
     6061 atcgtgtgtc atgttacttt atttatgtat acagatcaac gaaattatgc cccctcattg
     6121 gcaagaaaag aagaaagaat ttaaggaaga aatcgaaata aaatccaatg actggattga
     6181 atacacatgg cttcctctga aaagaaatag caaacgaaag gaattttggg cattgcaatt
     6241 atggccaata ggaaaacttt ttatttatat ggcttcactt atggttactt taatcggtta
     6301 tttggtggtg gtctatgggg tggcctatta cttgttaagt gagccaaaac agatcgcggc
     6361 gtcaaatatt ttcacggcag taatgacatt aatgtgtgtc atgtgctttt ctatttttag
     6421 ttacttactc gtccaattta tcaggcaaaa tttaacaatg agtaaacttt tcaaaagttt
     6481 attggactta cttatcttag gcagtatgtt aacgactcta acagatcggt tcgaacaatc
     6541 tgaaaaaacg gtagagggga taaagaactt cttaacgacg attgatcaat ttataaatgg
     6601 atatatgtat ttgtttgaag taggctttat tctcactttg tttggttgtg tatttataaa
     6661 actcattaaa agatcaatcg aaaaagaaat ggttattgat ctaattccga cgctgagcgg
     6721 ctatgagtat tacgttgatt ttggaaagta tgaggagttg atccatacgg atatcgttca
     6781 cacgagtatc catgaagtaa ccgttaaggg catcagatct actttaaaag atggacagga
     6841 gacgaaaaca atcgaccgaa gaaaagaggt ctacaatttt tataaagttg aagcaagtct
     6901 acgatatggc attgggaaat tgaggttaga tgggagtaat attcaacaaa acaaaccata
     6961 tgttttagga aaagtggtga tttagaactt cctatgaaag atgaataaat aaggagaatc
     7021 ttttgaaaat gattcttctt tacagaattg tgcttatagt tggcacggag ttaaaaatct
     7081 taaagaagga gcagttattc aaagtgaaag aacatcataa tcagttacta tgtattaaaa
     7141 cgggtacgat gaacattcaa taccgaacag aatatttaga attaacgaat caaactgatt
     7201 ttttgtcgat tcgagagcaa cataagcaag aaaaattaat ggctacttat agccaattga
     7261 ttgatgagtg ggaacaattc aaggaatcta aaacataaaa ttccatttaa acaccttatt
     7321 ttttaaagag cataaggaaa cgtgtaccca ctttcaaaat aaagtgggta gataggctgt
     7381 gcctatcacg tttctgaaaa aaataaatga ccccaaaatt ttcaatttat gtgtaggttt
     7441 atgggtagca aaaatatgaa tgtgggcagc aaaaagttca tttgtgggta gtaaaattca
     7501 aatgtgtgta gcaatgtgtg tagctacaca cattgctaca cacgctataa catatagtga
     7561 aaaggagata tttatggaag taaaaataag agatattgat gaaaaaacga ttcaaagaat
     7621 tgaccgaaga tgtattgaat taagtgaacg caccgggact aaatggagcc gaaatcgata
     7681 cctaaaagtt ttgattgaaa atgattttga ccatgaactt atggatcata gaaaagatca
     7741 atttgatcgg ttgttagaaa aatttgtgga tattcaaact tataatacga aaattttaga
     7801 agaatatatt gcaacaaata atcaattaat cgccttgtta attgaataat aaaaatagta
     7861 ttgtgaattt ataaaaggag gaaatcaatt ttggcaaaag caacaagaaa acatgtatat
     7921 ctttacgata tgcatatcga agtacttgat gaaataattt ctactcatga agatatatct
     7981 aattatagtg aagcagtcag acatttgtgt atgatgtatg atccaaaaat cgaagaaaaa
     8041 ataaatccaa ttcaaattaa attgaatgca atgggaaaag aaatttctat attaaccgaa
     8101 atggtttcag agtttgcgga tcttcatttg aaagatacag gaatactaat tggtacaaat
     8161 tcaactgtct atcaagaagc aaaagagcgt gtggaaggga aaataaaagc gaatcaaacg
     8221 aaaaagtatt ctcctaagat caagaaacca ataaatgaat ctgttcatgc cagagtagac
     8281 ccgtttgatc caacaaagtt tttaaaaaga taaaaagtag aaggggaaca tgatgaataa
     8341 atcgcctgca gtcattctca cttgtcaatt taccttgccc aataaaaaat ctttttcaac
     8401 atacattgat tatatgacaa gagaaaaagc gctggaagaa aaggaacgac gcacgccgga
     8461 cgaagaacag gaactgcaac gaatcaagaa tgctttagag gatttttata ttcctgaagg
     8521 agaaactttt tcggagagta agacagataa agaattcagt gaaacgtcga atgaagctca
     8581 atcgttgatg gaacatggaa tcaattttga caagttagag gaacaagatt ttaccaaata
     8641 tctttcttat atgacccgtc actatgcgtt agaacaaaag aaagaactga cgcaaaggga
     8701 acaaaaagaa taccaacggt tgaaccaagc aatgaaaaaa tatcaaacag agccacataa
     8761 aaaagcttca ggaaatcttc ctggagtttt ttctatggct tcagacgaag tgaaaggaag
     8821 tgatctgaag gagatcaaac aaatcttcca aagagggcaa caaaaaggct caatcattta
     8881 tcaagatgtt gtctctcacg acaattcgta tcttgaaaaa atgggcttgt acgatcccaa
     8941 aacagatacc ttagatgaag aaggattaaa agcagctgga agaaaaatga tggagacatt
     9001 attcgagaaa gaacaattga atgaaacagg ttactggatg gcaacgattc accgtaacac
     9061 taagcatatc catattcatt ttgcagttgt tgaaaaagaa aatacgcgta aaccttacac
     9121 ggaggttgaa aatggtgtga gttatatcgt accgaaaggt aagagaagac aagcaacatt
     9181 agacgcaatg aagaccaatt atgtacatga gttagaacgt ttttcttatg aaaaggatcg
     9241 aatgattggt atggagaaac gaaatttatt gacgcggaaa agtgatttgc gtaacacctt
     9301 aacaaaagtg gtaaaagagc cagcgcgtta cgatcgacac gcaatgaact tattaaatga
     9361 agtatataaa accctacctg acaaacgttc ggaatggcat tatggggatg aaaaacgaac
     9421 caaactttca ccatacacaa gagagaaact cgatgagtta accaaatatg tgttaacaaa
     9481 agaacctgaa tatcaagaat atatacagat ttcaaaatca ttgaaagaag aagcaaaaag
     9541 tctttatggg gaaagcaaga gagaatcaaa ggatgcagaa aaaaatgctc tctttgattt
     9601 gaagaagcgc acaggaaatg ccattctaac agaactgaag aaacaggata gtcaaataaa
     9661 acctttaata gaaagtatgg atcagatgca atctatttac aatgaacgat cctttcgaac
     9721 gcttaaacag ttaccaacga atgaatacaa atatagttac gaagaattca tgaagcgcag
     9781 gaaacaattc caacaaagag ttgtcacgca acggagtttg cgtcaattaa atcgaacgat
     9841 taatgcccat gaagaaaaat accacgcaag taaagcctac gaagagcaac aacgaagaat
     9901 tcaaatggaa caagaaagac agtaaagaca gtaggaggaa tagcaatgaa acaatattta
     9961 gatcaatgga aagttataga aggatcatta agagaagaac gaatcgaaca acttcccgat
    10021 tgtttagaaa aagagcattt gtttcagata agagaaatgt tgagaaacga acagtttgat
    10081 ccaaatcaat ttttagtcgt tgagtactca gctactggtg tgtactgttg taatcatgtg
    10141 aagggtgaaa aatattttat cattcaagaa tatgagggca agttagcgcc ttattatact
    10201 acttgggaaa tgaatgaaga ggggatcaat aacttcccgt gtaaaagcat tgaagagtct
    10261 atttcactca cagaatgtta gttgttaagt gaataaaaga agtaaaagag tcctgaacgt
    10321 aattgtccag gactcttttt ttcaatgaat tgttttccgc caattcaatg agtggtgcga
    10381 tttgcgacta cattatatca aaagaaagag aagggaggaa gaagaaatgg cttattcaac
    10441 gattggagtt gttgttgaaa aaagcagaga caatctcgtt tttgtgacag aaattcaaac
    10501 gggcagagct tttgtcgtca cagacaaagc ggcaaaagcg tatcaaagtg gcgatatttt
    10561 aacgttaaat ctgactacga agacgttcgt cgatgcggca gaagattatc catt
//
LOCUS       DAFEPQ010000081        10433 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.083, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000081
VERSION     DAFEPQ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.083"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            87..305
                     /locus_tag="LP543_002328"
     CDS             87..305
                     /locus_tag="LP543_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322467.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(594..1106)
                     /locus_tag="LP543_002329"
     CDS             complement(594..1106)
                     /locus_tag="LP543_002329"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBL2322468.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(1120..3417)
                     /gene="recJ"
                     /locus_tag="LP543_002330"
     CDS             complement(1120..3417)
                     /gene="recJ"
                     /locus_tag="LP543_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBL2322469.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3559..4002)
                     /locus_tag="LP543_002331"
     CDS             complement(3559..4002)
                     /locus_tag="LP543_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBL2322470.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(4003..4791)
                     /locus_tag="LP543_002332"
     CDS             complement(4003..4791)
                     /locus_tag="LP543_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL2322471.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4788..5729)
                     /locus_tag="LP543_002333"
     CDS             complement(4788..5729)
                     /locus_tag="LP543_002333"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBL2322472.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5906..6493)
                     /locus_tag="LP543_002334"
     CDS             complement(5906..6493)
                     /locus_tag="LP543_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBL2322473.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6714..8021)
                     /gene="obgE"
                     /locus_tag="LP543_002335"
     CDS             complement(6714..8021)
                     /gene="obgE"
                     /locus_tag="LP543_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBL2322474.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            8315..9325
                     /gene="gap"
                     /locus_tag="LP543_002336"
     CDS             8315..9325
                     /gene="gap"
                     /locus_tag="LP543_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBL2322475.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9410..10345)
                     /locus_tag="LP543_002337"
     CDS             complement(9410..10345)
                     /locus_tag="LP543_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL2322476.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2888 a         2356 c         1809 g         3380 t
ORIGIN      
        1 atcacttccc tctttacaaa ccgaacaaat attcgtatac taaatatacg aacaattgtt
       61 cgcataaatt aaagaggtga gaattcatga aatcaatgag acgtcttggc ttagtattaa
      121 ctgttttgtt tttaggtatt attattggta atttcggcat acgtccagct ttacttgtgt
      181 ttgttccgtt atttactttg tggttcatgt tgtgggatga aaagaagtac cgtcaatctg
      241 agcggcgcag aattcatgaa gatcatttct atcatgagcc ctactacaga tccaggcaat
      301 cataataagg attgactgct aattattcaa aagacaagaa gttaaacttt tacgggggtg
      361 tgacaaaaat cggtcacatc ctttttttcg aataaacggt gttctaaagc cagtttttcg
      421 gtattaagtc aaattgcgga aaaagtgatt ttttaaactt ctactggtct ttgcttgttt
      481 ccatttataa aaaaatagcg accctcaaaa gtcagataga gaatctgact tttgagggtc
      541 gctttaaaat cgcttcttta agaataacat caagaatagc ttcttttttc tgcttaatat
      601 tccattaatg taatgatgtc atatcctttg attttgtcgc gtccatggag atccatcaat
      661 tcgatcaaaa atgcgcagcc tacgactaca ccgcctaatt gttcgatcag ttcgatcgtc
      721 gctttgattg ttcctccagt agctagcaaa tcgtcacaaa tcagcacacg ttgacctggt
      781 ttaatggcat ccttatgcaa tgtcaatgta tctgttccat attctaatcc ataagtcact
      841 tcgatggttt ctcgaggtaa cttccctttt ttgcggactg gggcaaaacc gactcctaac
      901 tcataagcaa caggacatcc tacgatgaat ccacgggctt ctggcccaac taccatatca
      961 attcgttttt cttttgcata atttacgatt tgtttagtag cttcccgata agcgtcacca
     1021 tctgccatca gtggggaaat atctcgaaac acaattcctt ttgacggata gtcgggaata
     1081 ctggcaatat aatcttttaa atccactctt tgtttcctcc taggttgtta accatttctt
     1141 tatggtagga atatcgctca aaagcaaaaa ttcctcaact ttgattttat tgagccgtcg
     1201 ctggtataat ctgctctcag tcaaaggatg actttctggg ctagcgactt tttgtaaaac
     1261 accgttctct attgtaacaa atttcaattc aaaaaacacc tgtatcataa aaattaataa
     1321 tttttcaggg atacgcaaat attgagctat tgcttttatt ttatgacgta tatcaattcg
     1381 ttcttgagaa tggatcaatt taaacaattt tgcgtactgt tctcttgtgc ctaccccatt
     1441 caaataggcc tcatcaggtg aaatccccat cagataaaca cgattgaatg aataagaccg
     1501 aaaaacttct ttcagatccg ctaaatcatc tggacaatcc ataacaacta gagagtttgg
     1561 ttttcttttt tcaattattg cctttacaga atctagtcct tcgaaaacaa tcgcagcttc
     1621 ctggataatc tcaggcacga atttcaacgt cgaagaatca aatgctaaat aaaggctacc
     1681 ttccgacgtt cggctctgtt cacggaaacg ttttgcccgc caatcaaata cttgcagacc
     1741 acccacagaa aaatcagata ccatcagctg cggtttctta cgcccgttcc attcattgat
     1801 cgaaagcttt cctaccacat ctacctgatt attcagcagt tcttcttctt gagtaccaaa
     1861 accaaaagcg accgcatcaa gctgactcga tgcatcggaa aggacaaatt tcagatgctg
     1921 ttggtttgct ccgattcttt tgatattttc tgcttggact tgtcgaaata aaaactgagg
     1981 caaaggatta tccgtaccaa atggcgccaa tagtttcagc tcatcaattc gctctactgt
     2041 tacttcattt ggcagcaata cttcatcaat tctcaactca ggccctctca tcaagtcaat
     2101 ttgatgatcg actatatatt ggttcatctt ctcctgtagt atagtgacat tttcacttgg
     2161 catcgttaaa ccgactgctg catgatgtcc tccgaagtaa gtaaataaat ctcgcatctg
     2221 gtcaagcatt tcaaaaagat tcaaggcttc aatactacgt ccagatcctt tagctgatcc
     2281 atcttctttt aaagtaagga caagcgttgg tttacccgtt tcattcatta ttttcccagc
     2341 aacaatgccc aaaacgcctt catgccatcc tcggtttacc aatagatgga tatgattctc
     2401 cttattgacc atcgctaatg cttcttgtgt tatctgctct acgattgctt ttctttcttc
     2461 attgatctca ttcaattttt ttgcttgaac ggatgcttcc tcttcatcaa acgtcgcaag
     2521 taacgagacg gcaggacgtg gatcgcccat ccgaccgatt gcatttagtc tgggagcaat
     2581 cgaaaaaccg atcgttgttt cgtctgcgtc cctcattttt acgccgcttt cttcaaatag
     2641 cgcctgaagt cctatccgtt ctgaatgatg aatggcatcg attcctaatg aaacaaggat
     2701 tcgattctca tcagtcaatg aaactaaatc ggcgatcgtt cctatcgcta ctaaatccaa
     2761 aaattctgca ggcggttctt ccaataatgc agaagctact ttgaatgcta ctccgactcc
     2821 tgccaaatct ggaaatggat agtgccccgc tggatgacga gggtgaacaa tcgcataagc
     2881 gtctggtaaa ttctcaggta gttcgtgatg atctgtaatg atcacgtcta caccagcttg
     2941 ctgagcgtat gctaccgcat catttcctgc taccccgtta tcaacagtga tgatcaactg
     3001 gattccttct tcgatctttt cttggtatac cgattggttt ggtccatacc catgctcaaa
     3061 tcgatttggc aggaatactt ctacttctgc accaagtaat tccagtgttt ctttcattac
     3121 cgttgcactg gtgatcccat ctgcgtcata atcaccgtaa acgaggattt tttctccact
     3181 gattacagct tcttggattc ggctgattgc tttatccata tcgtgtatca gatacggatc
     3241 atgtaattct tgttcctttg gatgtaaaaa tcggttgata tccttttcct catggtaacc
     3301 tctttgccac agtaacttac cgacaaatgg agataatttc agttcacgga tcatttggag
     3361 aaaggattca gaaggctctg tctcgcttaa cttccaatca aaatttgctt gtctcacttt
     3421 ctcactccta acttagttat tatagcaaag gaaatcagca aaaaaaagca gaaagaaaga
     3481 ataaaaacag atcagatccg ctagacagat gttcagcagg acctgatctt ttgtacgacc
     3541 atttatgaaa tatccctatt agtcaaaata attgaagctt tgtttgggcg attgagtttt
     3601 ttgttcttcc ggcgctttca attgactgat ttgtgcggtt ttcgcttgta actccgcctg
     3661 ataagcagct tccaactccg cacgttcgtt tctaaactcg cgttgctgtt cttcttttgc
     3721 ttcttcaatt ttcttttgaa gttctgtttg atattctgac atttcctgag tcagctgttt
     3781 gatttgcttt ttttgctgcc atagtgttgc cgaagcagtc aaaaatacaa tcagtgcacc
     3841 aatcagcgca gaaccgatga tcactaagat caatggcgca gaaaaggaag tgaagccaaa
     3901 acttacagga acatttttat tgttcaatac agcaaaaaga acaataatca aaactaaaac
     3961 aaatccaaga atcacattgc tttgcttttt caattccttc acctactttt tgttaaaaat
     4021 tccgctggct aaataatcac caatcgttgg aaacagcgta taaaattttg atgcaacttc
     4081 taaaataaac ggttgattga tttcccgttt tggatgtctc atattgcgga cgatcatttt
     4141 tgccaacttg gcaggttcta aaacgatttg tccgactttt tccaaatagc ttccgcttgg
     4201 atcagcctta tcgaagaaat ttgtctcgat tggacctgga ttcactgtcg taacagctac
     4261 gccaagtggt ttcaattcta atcgcaaagc gttggagaag ccaaggactg caaatttcgt
     4321 ggcagaataa accgttgatt ttgcagttgc catttttcca gccatagagg cgatattaat
     4381 gatatgacct tgtcttcttt ctgccatatc gatcgcaaat ttttgtgtca aaaccatcat
     4441 tcctaagatg ttgacttcaa acatatcgta tgcttttcca aggtcgaatg taaggaagtc
     4501 ttcgaaaatc ccaaaacccg cattgtttac cagaatatcg acgcgcccga cttcttccat
     4561 caccttttca tataattcgt ccacgctttc aggattgctg acgtctacct tgaaagcata
     4621 agcctctttg ccgctcaagc gtctgcactc gtctctgaca ccttctacaa atgccattct
     4681 tcgtgcgcaa cttataacga ttgctccttg ctttgctgct tcataacaga tttgtgcgcc
     4741 cagaccgcct gaactacctg ttactaaaac aactttatct tctaaattca tgcctcttcc
     4801 tcctcgcgaa atggaatctc aatgctatca aaatctttca taatcgtcgt gttttcaaac
     4861 acttcttgtg cttctttttc aagctctaaa gctgcttttc ctaaatatct cgcactgata
     4921 tgagtaagta gcagccgttt tgcctgcgct tcttttgcaa cttcagcagc ttgatgagtc
     4981 gtggaatgga aatagttgtg cgccattctt gcttcatctt tattaaatgt gctttcatgt
     5041 accagaatat cgctattttc cgcaagcaca acggaattat gtgtttttct cgtatcgcct
     5101 aaaatagtca cgattcgtcc ttttttatct ggacctacga aatcttttcc attgatcgtc
     5161 cgcccatctt ctagttggat cgtttcacct tgcttcagtt tgccgtacag tggtccagct
     5221 ggaataccta attcttttag acgatccacc tgcaattctc ctttatgatc atgttcaacc
     5281 acacgataac caaaactgtc gatcccatga tttagttttt ttgtataaac agaaaattgc
     5341 tgatcagtaa aaatcggggc tgtttcattt agctcaatga atttcagcgg ataacttaaa
     5401 cgcgtttgcg aaatacctaa agagactttt atgtattcct ctatcccttt tggcccgtaa
     5461 atctctaatg gcgtatcccc accttgaaat gaccggctgc tgatgagccc gggaagtcca
     5521 aaaatatgat cgccgtgtaa atgtgtaatg aaaattttac cgatttttct cggacgaata
     5581 gtcgttcgca aaatctgcat ttgcgtgcct tcgccacagt cgaacaacca aacttcattg
     5641 cgctcgtcta aaagtttcaa tgctatactt gtcacatttc tatgttttgc aggaacacct
     5701 gcacctgttc ctaaaaattg taattccata atctacctga ctttctttaa gttcctcttt
     5761 aattttaggg taaaaagaag ggataatcaa ccgtaagtcg agaatgggtt caaattaaaa
     5821 ataacgttct aaagcgatag cgttttcttt gaaaattact ataaaaacag cagcgaaccc
     5881 gtttgagatc ctctgctgtt ttttattaat ttgaagatcc agaactttca ttgtcgttgg
     5941 aagtttgcgg atgtttgtcc ttccaagtct tttgtgcagc ttctttcgca tgattttcag
     6001 cttctttcgc atcatgcaca tctttttcga catcttcata atccaagcga gtaatttctc
     6061 cgcctaattc catcattacc aattgattca gtgggcgatt gtcctcttca tcagcaatca
     6121 agatcagtcc tgtagctcct tcaccgattt tatttcctac aaagtcgaac acggtctggg
     6181 catcttttat ttctttggca tcttgtgtcg caccgatcat tcctccggca aaccatccta
     6241 ataaaatccc tagcggacca cctaatacac caaccaacat tccgattaat ccacccgtag
     6301 aagatttgtt attaccggta aagtcgataa aatcttctat tttgaattga tgtgtcccat
     6361 taggatcatg gctgacaact gccatctgtt ctcctttaat cgaacgaaca gcatgtaact
     6421 ttttgatttc tgaaaatgcc tgatatgcct ggctttctac ttgaaaattt aaaatgatta
     6481 ctcgtttgct cattaaaaac gcccctttca taccttcttg ttatttattt tacctttaat
     6541 tgaaaaataa gaaaagaaaa gcactctttt tttccaataa aatagggctg tgacaaaagt
     6601 cgtatcacag ctcctttttc cgaataaacg gcgttcttga agctgatcac ttctgtcaca
     6661 acctccctta aatctgaatc tatatttttt ggtatttgtt tttatttgct tgcctattcg
     6721 acaaattcaa attcaaactc accgatacgt acaaggtcgc catctttcgc accgcgtgcc
     6781 cgaagagctt catccacacc cataccacgt aattgacgag caaaacgcat cactgtttca
     6841 tcatgatcga aattcgtcat ttggaataat ttttcgatct tctcaccaga taagatccac
     6901 gttgcatctg aatcacgatc gatttggaac tcaggacctt ctggattgaa tccatagtgt
     6961 acagtttctt cttccagttc ttcttcgtac aatgggaatt ctggcgttac ttctaacaaa
     7021 tctgctgtag cattcaataa cgcgtccaat ccttgtcgag tcactccaga aattggaaat
     7081 actgggatgt catcagcaaa ttcatcttct ttttccttgt tcagctgttc tttgaatttc
     7141 actagatttt ccggtgcatc cggcatatcc attttgtttg ccacgatgat ttgcggacgc
     7201 tctagcaagc gtaagttata agtagataat tctttgttga tggcaagata gtcttcataa
     7261 ggatctcgtc cttccatccc actcatatcg atcacatgta aaattactcg tgttctttcg
     7321 atatgacgaa ggaattgtgt acccagccca acaccttgcg aagctccttc aatcaagcct
     7381 ggcaagtctg ctgcggcaaa acttctgcca tcacttgtag taaccattcc tagattaggt
     7441 acgagcgtcg tgaaatgata agcgccgatt tttggacgag cagatgaaat aacggatagt
     7501 aaagtcgatt tcccgacaga tggaaaacct acaagtccaa cgtctgccaa tactttcaat
     7561 tctagttcga tttttcgctc ttgtcctggt tcaccgtttt cagcaatttc cggtgcagga
     7621 ttccgaggag aggcaaaacg gatattccct cgtccaccgc gtccaccttt tgccacaact
     7681 agtgtttgac cattttcaat taaatctcct aaaagagcac cagtctccgc atctcggaca
     7741 gtggttcctt gtggtacttt aacataggta tcttctgaac cacgaccgtg catcccttta
     7801 ctcatcccat tttcacctgg ctgcgctttg aaatggcgat tgaaccgaaa atccatcaat
     7861 gtacgtaacc cttcatcgac gatcaagatg acatcgccgc cacgaccacc gtctccgcca
     7921 gccggtccgc cgtcaggaac gtatttttcg cgacgaaatg caaccattcc gtctccgccc
     7981 ttgccagctt tcacatcgat cgttacttga tctaaaaaca tggacataat taagtcctcc
     8041 tattattcat ttatttattt gactgcctga atttccacaa taaacaaaag tcttacttat
     8101 tttatcggtg aaatctgatt ttgtctatca tttcagcaaa aagattgttt accttcttct
     8161 ttttttatca tttggtaagg tttccaacct tttttctgct accaagtata attttttgac
     8221 aaaatattct ttgcgtagca gaataacagt gtattaattg tattaattga ggatgccgtt
     8281 tcacagcacc cctaataaag gaggaacttt tgatatgaca gtaaaagtag gtattaacgg
     8341 atttgggcgt attggccgtc tggctttccg ccgcatcaaa gaagtctcag atgatattga
     8401 agtagtagca atcaacgact taacaagtcc aactatgtta gcacaattat tgcaatttga
     8461 ctctactcat ggtacttatc ctggcacagt tactgcaaca gaagactcaa tcgtagtgga
     8521 tggcgaagca actcgtgttt atgcagaacc cgacgcaagc aaaatccctt gggcaaaaga
     8581 aaacggtgta gatatcgtac tagaatgtac tggtttttat acatctgaag aaaaagcaaa
     8641 agcgcattta gatgctggtg tcaaacgagt agttatttct gccccagctg gtgctatgaa
     8701 aaccatcgtc tataacgtca atgatgatac attagatgaa aacgacaaaa tcatctcagc
     8761 aggttcatgt acaacaaact gtttagcacc tatggcttat ttcttaaata atgaatttgg
     8821 gatcgaagtt ggtacaatga caacagttca tgcctacact tccacacaaa tgctcttaga
     8881 tggacctgta aaaggcggta atttgcgtgc tgctcgttca gctgctgaca atactatccc
     8941 acattctact ggtgctgcga aagcaatcgg cttagttatc cctgagctac aaggaaaatt
     9001 acaaggacac gcacaacgtg ttccagtcgt tgatggttca ttaactgaat tggtttcagt
     9061 attgaaaaca aaagtaactg ctgatcaagt aaacgaagca atcaaaaaac acacagttga
     9121 caatccttca ttcgggtatg atgaccgtca aatcgtttct agcgatgtca ttggaacaac
     9181 tcaaggttcg atctttgacc caacgcaaac agaagtaaca actgctggtg atttccaact
     9241 tgttaaaact gttgcatggt atgacaatga gtatggattt acttgccaaa tgattcgctt
     9301 actagaaaaa ttcgctaact tgtaatttga tagatcaatc gataaaagaa gcctttgact
     9361 ggatccagtc aaaggcttct ttcttttcat cccctacttt taaactcgtt caatgaacat
     9421 attggcttaa aaattgttgg actacttgct gatatttttc aggatctgtc ttgtatgctt
     9481 ccgcatgctc ggctcctttt acaacatact tttcttttgg tcccttcgtt gcacgataga
     9541 ccttatctaa catttcaaat ggtacaaatg tatcttcgtc tccatgtata aagagcattg
     9601 ggcgctggtt tttcttcaat tgttccactg catttgcttc accgaaaaag tagccagcac
     9661 ggacttttgt cactagactc gttacttgca taagcggaaa tgctggaaga ctaaacatat
     9721 cgtctagctg ataagcaagc tcatcatgaa cggaagaata accgcaatct tcaatgattg
     9781 ctttcacatt atctggtagt ttttctccgc tagtcatcat aactgttgct gctcccatgc
     9841 tgacaccgta tagaacgatt tcttgcgatt ttccattgtt ttctagcact ttgtttatcc
     9901 actgtacata gtctttacgc tctggccatc caaatccaat gtaatcccct tcgctttttc
     9961 catgtcctct tgcatcagga acaaggacat tgtaacccat atcatgaaac atctttgcgt
    10021 aattggtcat cgtttcagcg tttcccatat agccatgagc cacaagagct gttttttcac
    10081 ttttcgtttc agcaggaaga taaatcgctg ataatttcag accgtctttt gaagtgatgg
    10141 tccaatcgct tcggttttct tttgaactaa accatttatg tgcatccgtt tctttggatg
    10201 tctgtgttgt accaggtgta tcttcgtcta aaaagtcttt ctcagaaggc acaacagcat
    10261 aattataaaa ataattccct gcaaacacta acgcaacaat cagagctaaa gcgacgccta
    10321 aaaggatttt catccaaagt ttcataaatc atccatctca tttctctttt ttcatataac
    10381 cttgaattag tataatcaaa gaatctctac tatgcaatgt tcatttttat aac
//
LOCUS       DAFEPQ010000082        10199 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.084, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000082
VERSION     DAFEPQ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.084"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            161..391
                     /locus_tag="LP543_002338"
     CDS             161..391
                     /locus_tag="LP543_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBL2322477.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="LP543_002339"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="LP543_002339"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBL2322478.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="LP543_002340"
     CDS             1802..1978
                     /locus_tag="LP543_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBL2322479.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="LP543_002341"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="LP543_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBL2322480.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2593..3060
                     /locus_tag="LP543_002342"
     CDS             2593..3060
                     /locus_tag="LP543_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2322481.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3145..4029
                     /locus_tag="LP543_002343"
     CDS             3145..4029
                     /locus_tag="LP543_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL2322482.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4143..4379
                     /locus_tag="LP543_002344"
     CDS             4143..4379
                     /locus_tag="LP543_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBL2322483.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4467..4547
                     /locus_tag="LP543_002345"
     tRNA            4467..4547
                     /locus_tag="LP543_002345"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4501..4503,aa:Tyr,seq:gta)
     gene            4554..4625
                     /locus_tag="LP543_002346"
     tRNA            4554..4625
                     /locus_tag="LP543_002346"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4586..4588,aa:Gln,seq:ttg)
     gene            4750..4920
                     /locus_tag="LP543_002347"
     CDS             4750..4920
                     /locus_tag="LP543_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322484.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5140..5847
                     /locus_tag="LP543_002348"
     CDS             5140..5847
                     /locus_tag="LP543_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2322485.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5907..6551)
                     /locus_tag="LP543_002349"
     CDS             complement(5907..6551)
                     /locus_tag="LP543_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBL2322486.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6678..6965
                     /locus_tag="LP543_002350"
     CDS             6678..6965
                     /locus_tag="LP543_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322487.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7073..7393
                     /locus_tag="LP543_002351"
     CDS             7073..7393
                     /locus_tag="LP543_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2322488.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7474..8787
                     /locus_tag="LP543_002352"
     CDS             7474..8787
                     /locus_tag="LP543_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBL2322489.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8784..9503
                     /locus_tag="LP543_002353"
     CDS             8784..9503
                     /locus_tag="LP543_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBL2322490.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9631..10182
                     /gene="raiA"
                     /locus_tag="LP543_002354"
     CDS             9631..10182
                     /gene="raiA"
                     /locus_tag="LP543_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBL2322491.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3400 a         1681 c         2178 g         2940 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact
     2521 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2581 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2641 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2701 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2761 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2821 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2881 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2941 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     3001 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     3061 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3121 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3181 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3241 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3301 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3361 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3421 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3481 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3541 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3601 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3661 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3721 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3781 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3841 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3901 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3961 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     4021 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     4081 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4141 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4201 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4261 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4321 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4381 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4441 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4501 gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta
     4561 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
     4621 cccagtatac cacaatcgta agctctcgtt agttcgagag ctcttttttt tgctttgcta
     4681 ccttgttcct tgaagattct gctgatttaa gatagagtta agttaactac aagaattgga
     4741 gggatccaca tgaaatggaa agaaaaagta gacgaagcag ctgaaaagtt atatgatttg
     4801 gtcaaatcag aaaaatacaa tattgaagtg aatattccga aaaaaggcgg aaaagcggtc
     4861 caggtaaaat caaaacgccc aacgaatcat acaaaaaaat ggatggctaa aaaccgctaa
     4921 taataacaaa agatccataa gtcggcattt tcatcgattt tagggtcttt tgttattttc
     4981 taggttgaac atatctaaaa aattgtttct gacaatagga gtaggaagaa cgatgtgtgt
     5041 ctaaatcctc tgttgatgga tagaaacctt tatctttttc agttgtgaat caggtatata
     5101 ccagttataa tagaagaaac gaaaggacgg gtgtacgtta tgtcgaatca attacctgta
     5161 tatatacaga ttcatgacca attaaaaaat gaaatcgaac aaggtatatg gaaagtcgga
     5221 gaccgtcttc cttcagaaag agaattagca gtgaaattca atgtcagccg tatgacgttg
     5281 agacaagcaa ttcaaaactt ggcagatgaa ggaatcttag aaagaaagat cggttcgggt
     5341 acatatgttg cgcgagaaaa agtacaagaa aaaatgtctg gtgcaacaag ttttacagaa
     5401 attatggaat cgcaaaatcg agtaccgtct agtcggacaa tctcttattt cttgacttcg
     5461 ccaagctcaa gtgaaatgga aaaactaaac ttgagtaaag atgacacgat cttgcgaatg
     5521 gaacgtattc gtttcgcaga tgatgtccct atttgttttg aagttgccag tgtgccacaa
     5581 aagctgatcg atggctacag caaagcagag attactgaat cgttgtatcg cacgctagaa
     5641 gaaaaaggcg ggaaaaagat cggtccagcg aatcaaacga ttactgctgt gttggcttct
     5701 gagaaaattg ctgactattt agaggtaaaa cgaggagatg cagttcttcg tttgcgccag
     5761 atttcttatt ttgaagacgg tacgccgttt gagtatgtgc gtacgcaata cgtaggaaat
     5821 cgttttgagt tttatttgga aaaataaaaa acaggtgatc cctgattcct tacttagaaa
     5881 taaaagagaa tcagaatcac ctgtttttat atatgatcag ttaccagttg ctcgtgataa
     5941 gaaacaggac cctcttcaaa ttggacttgt ccgttcagca gctcaatgat ggattcttta
     6001 aagtggtcag cacttttttc atctacagca cagatgacgc taacttgatc agtatattgg
     6061 atttctttta taaaaaaatg ttggagttca agataattct gtaattttcc tagttgcgga
     6121 tacgtgatca tcacagagat ctcctgttgc aaggtacctt ctacgattcc aatgtcagac
     6181 aatgcttgag agacagcatg agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt
     6241 cctccaaaat atcgagtgac tactgcagct acattgatca gttgattttt tttgaggact
     6301 tccaacatcg gtacgccagc cgttccactt ggttctccat catcgctgct tcgctggatt
     6361 tcattttttt ctccaatgat gaaggcgctg cagttatggt tcgctttcca atgttctttt
     6421 tttatttctt gaatgaaact ttttgcttgg tcctctgatt ctattcgtct aagagaacag
     6481 ataaagcgtg attttttgat ctcaatctca ctttgtccat tttcttttat tgtacgatag
     6541 cgctcgatca tcttgtcacc tctttttttc attttcttta ttcagtataa ataaagttct
     6601 aggttgtttc aacactgtaa cggttgcgca atcgctgtag tatgcgataa taaagacaaa
     6661 ggtaggtgga tctggggatg caggaacgaa tgaaaaagta cgatgcaatt acgcattatt
     6721 tgaagaacaa tggcggctct caagtaacgt tgacctttac ccaatttgac gaactgctgt
     6781 ttcccagtaa cggacttccc aaaacagcaa gggaaagtac agattggtgg gcaaatgatt
     6841 ataaacaccc tgaaaaaggt gcctatggct ggataaatgc aggctatgaa gtagttgtca
     6901 tcaatttgga caaagaatat gtcgtgttca ataaattagt gaaatctagt tggctatttg
     6961 attaaataaa ccgcgtttag aaaacgttga caaatttctt tctttttata taatagagta
     7021 acagacgcga agaaaacgtt tgcgtagttt gttttaaaaa ggagagaaaa gtatggctaa
     7081 aaaaacaatc atgcttgtat gttcggctgg tatgagcaca agtttactag taacaaaaat
     7141 gcaaaaagct gctgaagcaa aaggaatcga ctcggatatt ttcgctgtgt ccgcttctga
     7201 cgcagataac aatcttgcaa ataaagatgt agatgtactg ttattaggac ctcaagttcg
     7261 ctttatgaaa gctgattttg aaaaacgtct ggaaccaaaa ggcattcctt tagacgtcat
     7321 caatatggct gattatggta tgatgaatgg cgaaaaagtg ttagatcaag caatcacgct
     7381 cataggtgaa taaatagaac agtataacta tcgaacaagc gatccaaaaa atagatcgct
     7441 tgttttttcg tatatttttt tagggcgaat aatatgaatc atttttgggg tagaaggctt
     7501 ctgacgagag atattaaagc aatagactgt gaggaggtaa atttaccaaa ttatgagaag
     7561 atcgcagcag tggaacgttt gggcaactgg attctttgtc atcgctgtgg aatgaggacg
     7621 tccgtcattg atggacagct tgcagattac ggttattttt gtacacattg tctatattta
     7681 gggcgatgcg acactcaaca agatttatat ttgtttgatc agcctgaagc aaaacctcga
     7741 gaagtcgtct ttttttggac aggacagctt actgaaaaac aaacggaaat cgcaaagaga
     7801 atactttgcc attcagaaaa aagacatcat cttatttggg ctgtaacagg tgcagggaaa
     7861 acagagatgc tttatcctat tttagtggaa actttaaaag ctggaggaag agtagcgatt
     7921 tgtacaccgc gagtcgatgt ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca
     7981 gaagaaaaaa tcacgttgct ccatgggaat acagcaacgg cttatacctt tacttctttt
     8041 gttatctgta cgattcatca gctataccgt ttttatcggt cctttgattt aatagtgatc
     8101 gatgagatcg atgcgtttcc ttataacggg gatgcaggat tagctcatgc tgtgcaaaca
     8161 gcgattaaac cagacggacg attcatctac ttaagtgcca cacctgataa aaagttacta
     8221 aaggaaatca agcagacgtt tgacatccac aaacttgctt tgcgctttca tcggcgttta
     8281 cttcctgaac ccgtcttggt tttcatgaat aattggcgac aaaaatgcaa gaacaagaag
     8341 aagataaaat ctttggtgcg gatcattcat caattaatta aagaaaacca tatcctttta
     8401 ttttgtccaa gtatcacgat gatgcatcgt ttgaaagcca cacttcaaga ggctcttccg
     8461 agatatgaga tcacagatgt ttcttcaaga gacaatcagc gggcggaaaa agtccagaaa
     8521 atgcgagaag caaaatatga catactgtta accacgatga tccttgaacg tggagtcaca
     8581 tttgaaagaa tatccgttat cgttttagga gcggatcatc cggtttttac taaatcttct
     8641 ttggtacaaa tcgcaggaag agccgatcgt aaagggcctt atacgaacag tcaagtatac
     8701 tttttctatg aagaaaaaac tgcagctatt cgaaaagctt gtcaggaaat caaagaaatg
     8761 aatgaagaag ggaagaagat gctgtgaggt gcagttgctg cagtaaagat atcgtccaaa
     8821 atttaacatt cagcgagatt attggtgttt cgaaatacag attacctcag acttgttcag
     8881 tttgtcgcgc acaatttcat ttattggaaa caagaggctg ttctggctgt caaaaaccaa
     8941 tccatttcaa tctagagagt acgaaaaaga aagtaaagtc tattcccgtt tactgcagtg
     9001 actgtcaaga atggaagaag aaatatccga cctatgattt tcagcaccgt gccttttttg
     9061 tgtacgatga aaatatgaag gcctggctca atcaatacaa gtttatggga gatatccgac
     9121 tagcaggcac ctttgcatat aaatggacgc agataaaaaa agaatacccg acatacatct
     9181 attgtccaat acctttatct gaaaaaaggc gaacagaaag aggattcaat caagtaagcg
     9241 agatgttgaa agcagcaaga attccttttc agatgctact caaaagagag aagcatctgc
     9301 tggctcaagc aaaaaacacg cgaaaggaaa gattggagat gcctcaacct tttgaacttg
     9361 cagtcacctt ttctaagata caaaataaaa aaatattgat tattgatgat gtctatacaa
     9421 caggacagac tcttttccac gcagcagatt gtttgcaggt cgcacagccg aaaatcatcc
     9481 gaaccatttc tttagcaaga tgaattagat gtggaaattt gatagcgatt taatttgcaa
     9541 ataacctaga gttcgatata atagaattaa gaagagggaa agagtggtcc ttctccttct
     9601 tgattgtggt agacgaaagg ggtaattttt atgtttagat ataatgtacg cggcgaaaat
     9661 atcgaagtaa ctgaagcgat tcgcgactac gttgagaaaa aagtaggcaa actagaacgc
     9721 tatttcagtg acgcacccga tgcaacggcg catgtgaact taaaagttta tacagaaaaa
     9781 acagcaaaag tggaagtaac gatcccatta ccttatcttg tattacgtgc cgaagaaact
     9841 tctccagatt tatacgcaag tatcgatttg gttgttgaca aattggaacg ccaaatccgc
     9901 aaattcaaaa caaaaattaa tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct
     9961 actgctttat tcaatgaaga ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt
    10021 acgaaacgcc tttcattgaa accaatggat agtgaagaag cagtcttaca aatgaacatg
    10081 ctgggacata acttctttat ttttgaagat gcagaaacaa atggtacaag tatcgtttac
    10141 cgccgtaaag atggaaaata cggactgatt gaaacagact gatcagcctt acttaaata
//
LOCUS       DAFEPQ010000083        10150 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.085, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000083
VERSION     DAFEPQ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.085"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            181..1242
                     /locus_tag="LP543_002355"
     CDS             181..1242
                     /locus_tag="LP543_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL2322492.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            1560..2150
                     /locus_tag="LP543_002356"
     CDS             1560..2150
                     /locus_tag="LP543_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HBL2322493.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            2177..3487
                     /locus_tag="LP543_002357"
     CDS             2177..3487
                     /locus_tag="LP543_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBL2322494.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            3484..4203
                     /locus_tag="LP543_002358"
     CDS             3484..4203
                     /locus_tag="LP543_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBL2322495.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            4218..5303
                     /locus_tag="LP543_002359"
     CDS             4218..5303
                     /locus_tag="LP543_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL2322496.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            5439..6971
                     /locus_tag="LP543_002360"
     CDS             5439..6971
                     /locus_tag="LP543_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL2322497.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            7149..7370
                     /locus_tag="LP543_002361"
     CDS             7149..7370
                     /locus_tag="LP543_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBL2322498.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
     gene            7952..8569
                     /locus_tag="LP543_002362"
     CDS             7952..8569
                     /locus_tag="LP543_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL2322499.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            8566..9096
                     /locus_tag="LP543_002363"
     CDS             8566..9096
                     /locus_tag="LP543_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBL2322500.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(9962..>10150)
                     /locus_tag="LP543_002364"
     CDS             complement(9962..>10150)
                     /locus_tag="LP543_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL2322501.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
BASE COUNT         3627 a         1102 c         1936 g         3485 t
ORIGIN      
        1 catacttacg aggatcatat tattgattta ataaataatt taaaggaaat aatttttcta
       61 gaagaagata gtatgtcatt cacatcatca agatcagagt ctattttttt cagagaattt
      121 aagaaaatcg ttgatgccgt cattgactaa ttgagaaaac gtgagtgaaa ggatagaagg
      181 atgaattcga gtgatctaat tagtgtaatc atacctgtat ataatgttga aaagtatgta
      241 ggaagttgtt tggaatcagt gtgtaatcaa acatataaga acctagatat tataattgtg
      301 gatgatggat ctaaggattc atctaataaa atatgtgaag actatgcatt aaaggattca
      361 aggattcgta ttattcataa aaataatgct ggcttgggat tggcaagaaa tacaggactt
      421 gaacatgtaa aaggaaaata tgttgctttt attgattctg atgattttgt tgcagctaat
      481 tatattgaag caatgttaaa tggtataaaa aggagtaatg cagacacatg ttatgctgga
      541 tattatgaat attatgatga taataatatt atttcaaagc cagcaagata tgatagtacg
      601 atatttacaa ataacgctat aattgaaaaa gtgttgcttg gaatgattgg cactgagccg
      661 agttataaag tcgattttat attgccaatg tcagtttggc atgcgctgta ttcatctgag
      721 ataattcaac agcacaatat caagtttccg tcagaaaggg attatatatc tgaagatatc
      781 atattccata tttcatattt gcaacattca aaaataacaa gttttattaa agattgtatc
      841 tattattata gatgtaataa taataattca ttgacacata attttagaga taatgagttc
      901 gattgtaata ttgaattagt taaaaaaatt gaaggcgatt taagctatat attagaaaat
      961 gagatatttg taaacaaaac aaggagactt ttgttgggac gctgtcgtac ttgtattcaa
     1021 aaggcagtta gatatgctag ttttaataaa gaatttgatt taaactccta tttgtctaag
     1081 atacttatga acgatgagtt gattgcctgt attcactcat acccatataa acaaaatcca
     1141 attaaacaac gaatatttaa ctatttttta ctaaaccaat ccattattgg ggttaaaaca
     1201 ttagcatatt taagcggaat gggtagaaat aggagatttt aatcataaaa aactgtacta
     1261 tagtgaattt ttggagattt atattccagt tatatattaa tgattgtgat acttgtacta
     1321 ttgtaaaaat tcgttaggtt taaataactg gaaatatcgg aaaatgggca ttatacatca
     1381 cggacatccc ttcttttgga aaaaatgtta cgatttttat aagtaatatt gtatccggtg
     1441 gaatcaacga tgaagataac atatccatcg ttgtagattc agtagcattg aatgatgctc
     1501 ttgttgcaag aaatccgaat aaaattgtta agtataaatt gaattaatag gagatatgaa
     1561 tggaaattag cagtaaagaa tatttaaaag cggttcttcg tatggaaaag aatctatata
     1621 tagaatctaa aggacaacac acaaaattac taaaaataat gaaatcacat ccggactaca
     1681 acgcttggcg gtatactcgt cgaatgagaa ttacatctta ttattattca aagagaaaga
     1741 aaaatatcat ttatgcgatt atgtatattg tgtcttgcag aagaatgaat agtttgggaa
     1801 gaaaatgtgg tatagagtgt ggtgaaaatg tatttgatga aggtgtaaga ttttaccata
     1861 cacaaggaat tgttattaat ggtaacgcca taataggaaa aaattgttac ttatatggga
     1921 ataactgtat tggaaatgat ggaatcgatc ctaagtgtcc tgtaattgga aataatgtac
     1981 gtgtatgcgt gggtgcaaag attattggta atgtaaaaat agctaacaat gtagttgttg
     2041 cggcaggagc tattgttatc aaagattgtc tagagaatgg tgcaattctt gcaggtgtac
     2101 cggcaaaaat aatagggtat gttaatttaa caaaagtatt tttagaataa tttttagcga
     2161 aagaaggtga ataacgatgc gtatttcaat aaaaagatgg tcaggagcag attggacatt
     2221 gttcacgttg gcattttttg caatctccat agttcttgta tatactttta acgccccaag
     2281 agcaatttta tatttgggag atataattaa tatctttgtg ttattaaatg cacttagctt
     2341 tagacgcaga aaaggaatat atgctagcaa aaatgagatg ctgatagttt tgtttttact
     2401 agtttctatt tgtagctcag ttattaattt agaaaatata ggattgatta tctgggggat
     2461 acgaaataac actcgtatgt tcatagtata tataaattgt gcaacatatt taacaattaa
     2521 agattactat aaagtattcg ctataattga aaaattattc tggatttcac tgccactttg
     2581 tttaattgaa agattcatgg tcacctatcc aactggaaca attgtaggag atatgatagg
     2641 tggagttttt tggaattatt caggttgtaa tatgccattg aatttaattt tgtgtgtgac
     2701 agttactgtt gttacaatcg gatatttcaa gggagaaaaa aatttcatcc attttatttt
     2761 atgtgtttgt gcagcacttt ttatgtctgc attagctgaa ttgaaagttt ttattcctga
     2821 attatttata atatttgttg caatagtatt cactttaaaa gttgatttta aagtagtgat
     2881 caagatatta tcatgcgtaa tacttgtgtc gatagtatta tcctttttta taacaatatt
     2941 tattacattg aacgataatg gacaaagcta tgctgataat ttcacaattc aaggtctttt
     3001 agaatatgcg tcaagagatt ctggatatga tggtgttggt gatttaaatc gaataactgg
     3061 tatctctaga gtagtagatg aaatatttaa taataatttt agattagtcc taatgggagt
     3121 aggcattggc aatgcagagt ttaccaattt ttttgtcagt gagttttata aatcgtttgg
     3181 taatcttcat tatcagtatt ttcatcatat ttggttattt atagaagttg gaatattagg
     3241 agtttcgata ttcctatttt ttataataaa gacatttttc aatgcaaaaa gatcttttat
     3301 gaaggataaa gatgcaagtc ttgttaaagt tattgcaatt atgtcggtat tcttttttgt
     3361 gtataacaca acacttaggg gtgaaacaac ggggccactg atttatatga ttttagccat
     3421 gcctgaagtt gtgaaaagga aagagcatta ttatagtgaa aaaaatacaa aaggagagat
     3481 attatgattc ctaagaagat acattactgt tggttcgggg gaaatccact tccgaaaaaa
     3541 gatagaaagt gcatagaaag ctggaaaaag tattgccctg attatgagat tattgaatgg
     3601 aatgaatcaa attatgattt aaataagaac cgttatatgg ccgatgcata taaagaacgt
     3661 aaatggggtt ttgttccaga ttatgcaaga tttgatatta tttataatca tggtggattt
     3721 tatttagata cagatgttga gctaattaag tcgttagata tacttcgtga aaatcatgcc
     3781 tatatgggat ttgaagggga aatctgggtt aatggcggta ttggttttgg tgcagaagaa
     3841 ggacattctg ttattaaagg tttaagggat atttacgatt cattaagttt ttatcaaaat
     3901 gatggtacat tgaacttgaa accgagtccg tattatatta cagattatct tgaatctatt
     3961 ggattggttc gcgataacag cgaacaaata ataaatggcc tgcatatcta tcctacggat
     4021 tattttgctg ccaaggatta tgatactgga aaaatttata aaactagtaa tacaataagc
     4081 attcatcaat ataatgcatc ttggaaaaca cctaaacaaa aatttgttta tagattaaaa
     4141 tcaacaattg gacaaaaaaa atttaacagg ttacttgcac tgaaaaaatt tttacactta
     4201 taaaggagaa aagtactttg aagaaaattt gtttcattag tggtgtaatt tctcgttctg
     4261 ggggaactga aagagtaggg agtattattg ccaatagctt gtctgattta ggttatgatg
     4321 tcactatttt aagcttttgg aatagtggag agccttattt tgggttagat aagacgatta
     4381 aggttgatta tatgttaaat cctaaaacgg agggaaaatt atatagaact tatttatatc
     4441 cggtttggaa attgcacaag tatatatgca aaaatgaatt tgatatagta attgatattg
     4501 atactttgtt gtctttgtat actacaaggg caattaaagg aacaaagtgc aaattgattt
     4561 cttgggaaca ttttaattac tggacaatgg aaatgctgaa agaaaagaag agattcaagg
     4621 ctaaaaaatt gataaaaaaa tatgcaaaga ggcttgtggt tctcacagaa gaagatcgtc
     4681 aaaaacatat tgatgaatat agtttatcag aagattttgt tataactatg ccaaatccgt
     4741 gtatatctaa tatcgagtac gaatatcatt atgataaaaa gagatttctt gcagtagggc
     4801 gattagtgga agagaaaaat tttgctgaca tgataagtgc ttggaaaatt gtagaaaaaa
     4861 aagtagatga ttgggatctt atcattgtag gaaaaggtga attagaaaaa gatcttcgcc
     4921 agcagataaa agatttaaat ctgcaaagag taactatagc agggcattca gaccatgtgg
     4981 aggaatatta taaatctgca tcttgttatg tgcttagttc tgtttatgaa ggatttccta
     5041 tggttatatt agaagcacag tcttatggat taccagttat ttcttatgat tgtaaaactg
     5101 gtccgagaga tttggtacat cataatttta atgggatgtt ggttgaagat aagaatatag
     5161 accaattagc taagagcatg ataatgttta ctaagaatac tgatttagca atgagaatgt
     5221 cactgaacgc atataataat gtacaaaaat ttaatttgaa agaaattaca aaacaatggg
     5281 ttgctttgat tgaaaatgta tagttttttg tggttgtcca aacatgaaaa ttagcgtaat
     5341 taatcaaaga gctagaagaa acctattaca tttcaagatt ttagaaatta tatagtatta
     5401 ggtaacaata aaaaatagaa agtttggagc catatgatat gataattaat cgtaaacaaa
     5461 atgctaaaag gaatataatt actggaacaa tttttaagtt atatcagatt atcattccct
     5521 tttttatgcg tacacttatg atttatcaga tgggaatgga gtatgctgga ttgaacaacc
     5581 tgttcacatc tatatttcaa atgttaaatt tagcggaatt gggaataggt gctgcactaa
     5641 ctgtaagtat gtataaacct atagcagaag acaatagaaa agagatttgt gctttactga
     5701 acgcatataa aaagtacttt tcaattattg gatggatagt gtttacatta ggattgttat
     5761 gcattccttt tttgagattt ttagttaaag gttcgattcc tggaaattta aatatgacag
     5821 ttttgtattt aatgtatttg ataaatactg ttttgtcgta ttggttattt tcatatagaa
     5881 agagtctaat atatgcacat caaagaaatg atgtaattaa cgtgtctatg ctaattactt
     5941 ttacattcca atttgtaatg caggcatatg ttctgatttt cttaaaaaat tactatttgt
     6001 atttattggc ttctattttg ggacaaatag ttcttaactt aatgtgtgct gttgtttctc
     6061 aaaagatgta tcctgattat actcctgttg gtgagttaga gaaagaaact attgacgata
     6121 ttataaaaaa agtaaaagga ttagttacaa acaaggtagg cggtacaata ttacgatctt
     6181 cagattcaat tattatttca tctttcttag gtctttcagt acttgcgatt tatcagaatt
     6241 attactttat tatttctgcc ataatgagta tatttgcaat aatttatgaa tcatgtatag
     6301 caggtatagg taacagtctt attgtagaaa gtcaagaaaa aaattatata gatttttgtg
     6361 atattacaac aattataggt tggtttctgg caattagttg tgcatgcttt attacattat
     6421 ttcagccgtt catgattatc tgggtgggaa gtgataatct tatgagtatt tcatttgtta
     6481 tatttatgat agttcatttt ttcttatatg aaattgatca gttaattggg ttatacaaag
     6541 atgctgctgg aatatggtac gcagacaggt ttagaccatt aattacagca atgataaaca
     6601 ttgtattaaa tatcatcctc gttaattggt tcggattata tggagttttg gcggcaacca
     6661 ttgtttcagt attgttggtt gatttaccct ggttattata taatgttttc caaaatatat
     6721 ttaaatttga atcggcaggt aaatatgttt ggaagctcat aagtttgtgt gtaatagctt
     6781 ttgttgcttc ttatttaagt tattttgctt gttcgtatgt taatatcgga aatttcaaag
     6841 tgataagttt agtgataaat ttagttatat cggttgtcat atcgatttta gttattcttc
     6901 tgtttaatat ccgtaataag aagtttaagt atattttgaa agttcttacg tcggtattgg
     6961 taaaaagatg agaagagaca tcagaatgat atataaagta actacaattt ttaggctgcc
     7021 gaataggcga acgatgtata gtttttgttc caacgaagac tcttgttgaa attggagatg
     7081 atatgaagga gttacaattt tgactcatgt ttatgattga tcggttttaa aagtagtatg
     7141 gagaagcttt gggatcatcg gagaggatca aaataggtaa taatgttttt ataggaatga
     7201 agtctagaat actaaagggt gtacatatag gaaataatgt agttataggt gcaaatagtt
     7261 tagtaaacaa ggacgtatca gacgattgtg ttgttgcagg aaatctagca aaagtaatta
     7321 tgtccctaga tgaatattat aaaaagataa acaatatgaa gaatccgtaa aacttgtgaa
     7381 actataccga gaataatatg ggaaaaatat tgatgaaaaa gcattatatg agtttttagg
     7441 gctgtttaca gatggagatg acaatttgtc gaagagttgg gagcaatgca aaatttagta
     7501 ggggattata aacagactga caaggtattt aaagaacatg cacaagtatt tcataatatt
     7561 gaagaatttt tgaatactgt gagataggag ttgctacatt gagaaaagta tgtatttttc
     7621 caatgtaaaa gattacgagt ttgaaaacga caccataata ttatttttta agaagatatt
     7681 atagagctta ggaacggaag tttatttaag gatgttgaat aaaatgaaaa ataagattag
     7741 gtggatgaaa aaggcagatg attttttact ataattaaaa atattgttac tatgttatca
     7801 atcgtataaa aatacttact cgttatcaag acgattattt cgaatatttt aggaaaaata
     7861 aattgaatat tagagaatgt gataatacac tctaatatga tcatgtgtaa ttggaagcga
     7921 taagtgtata cttgatattg gagaaataat tatgataata gtgattgcag aaattattac
     7981 agatatatta tataaattat atgaaccctt tgggtttgct gttttattga caattttagg
     8041 actggtctat tttatgtatg caacagatgg cattgctgca ggtgagggct ataaacgtat
     8101 actaatggaa ctttatcttc atttaaagaa ggatagaaaa tatagaaaag tttgtgcttt
     8161 gatattctat actgcgatga ttttatttac aacattaatc aatcgaacaa tttggttaaa
     8221 tcctttatct gatgttatgg gagactggtg gatttataag tataatacaa caactggaac
     8281 aaaagagtta tctacagcgt gtttagaaaa tattattctt tttattccat atgtttatat
     8341 ggtaatgtct attaaatatg aaaatataaa gaaacccaaa atatggtcct caatagtata
     8401 tggaggaaaa attgcatttt atttttcagc aataattgaa tttttacaac tttttttgag
     8461 gctaggaacg tttcaaattt cggattttgt atataatacg ttgggcggta tattgggtgt
     8521 aatcctttat aaactaattt tgattacaaa aagagatggt gaaagatgag actcggattg
     8581 ataataaaga atatattaaa tttacctaaa acgatatact tcaattataa atttcttcat
     8641 atttcagatg cgtggagatt acctattttg gtgcatcgta aagtacggat tacacgcaaa
     8701 tcgagaggga aaattgagat agtattacaa tctggaaaaa tagtttattt gggctttcca
     8761 gtattaaata ctgcgggaaa tgaagaaacg attataagta ttgaaggaat actaagaata
     8821 caagggccag cgtttatcgc acaaggggct catattgaca taaggaaaaa tgcagtttta
     8881 gatattgggt caaagtttaa ttcgacagga aaaatgagta taatttgctt taaggaaatt
     8941 caattcaaag atggctgtac agtatcgtgg gatacattat ttatggatac cgattcacat
     9001 acgatttttg aattgaatac tcataggatt ttgaataaaa ataataaaat agttataggt
     9061 aaacattgtt ggattggagc aaaaagcgtg atatagaaag gtgttcattt agcagataat
     9121 attgttgttg cttgtaattc cacggtagta aaaagtcatg aaaataataa tgagattata
     9181 gggggggctc cagttcgaac gttgaaaaga aatacaagtt ggtgtttgaa cgagccaaag
     9241 tgaaaccaaa taattataaa actatggaag tataataaag tttttgagaa atttgaacga
     9301 caagacccta aaaaaatcca tggaccatta ttatggatgg acttcccaaa aggaatagcc
     9361 acttttgagc acgtaaatcc cctaataagt ggatcgacat cgcttgtgca gcagatgata
     9421 ctatcaagaa aggtgtttat aatgtagaac ttgaactaaa tagatttgta atcacccatt
     9481 aggattgtaa agaatatgaa taggatatat cttaagatga aaaaatttgc aaattctaat
     9541 gtatatttat taagggcact ttgctgtgtt tttttcagag ataagatgaa agaaagatgg
     9601 aagtcttgga gtatacgaaa taaagaaaaa tattatatct gtttaataag ttgatattga
     9661 acaaaggact agtcggagtc agcaacacat aagttgtgag tgatgctgac tcgtttttgg
     9721 cagaaaagaa tcgaatagta agtaggtgtt ttgatgttgc ttgacaatgg agagagaacc
     9781 caagaagatt actagcaaag cttatagatg ttactgtaga tttttaactc cagatacaga
     9841 gtttgatgaa tattctgatt atatcaataa cactataaaa aaggaaattt ccttttcaca
     9901 ggagaaaggt aaaagatatt ttcataggag aatcaatgtt aattttaaat tatatgattt
     9961 gtcaaattaa gctagaataa aatgcttctt gtacatagct caaaaatatt tcgattggtg
    10021 ttctgtaatt caatgatttt cttggaatat gattgcgttg attgctgaca ctggaaataa
    10081 atgtctgatt cacttctcta aaatctattg atttcggcag tccattacga cgcagaatcc
    10141 cgttagaatt
//
LOCUS       DAFEPQ010000084        10070 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.086, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000084
VERSION     DAFEPQ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.086"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(40..1419)
                     /locus_tag="LP543_002365"
     CDS             complement(40..1419)
                     /locus_tag="LP543_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HBL2322502.1"
                     /translation="MTKVTLKKVNPPKKDNEAKIYQLTDETMKFRKEKVLAAMEKSEI
                     DTLVIYCDLEHGGNFSYLTGFVTRFEESLLVLHQTGEAFLVLGNENTKMVDYSRIEAE
                     LIHTPLFSLPDQPMDNEKPLTEVLTNAKISKGKKVGLVGWKMFTSEEDNYQIQDLPAF
                     IVSSIVELVGEDAVSNRTDLFIHSDYGVRSLNTANEVAYYEFGSSLASDCVMNAIDEV
                     EVGKTEMEIGNHLVKYGQPNNVIPIAATGERFENAYIYPTDKQVKLGDKMSITTGFKG
                     GLASRSGYAVATTDDLPEGQKDYIEKVAAPYYDAVVAWLENIKIGMQGKELYSVIGKV
                     LPKSEYKWHLNPGHLTADEEWMSSPMKKDSEITLKSGMLLQIDIIPSVAGYAGASCES
                     GIALADESLRDQLAKEYPNVWSRIISRQEYIRNELGIELPDEVLPLSSGVGYYTPFFL
                     EKNLALVKG"
     gene            complement(1440..2987)
                     /locus_tag="LP543_002366"
     CDS             complement(1440..2987)
                     /locus_tag="LP543_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2322503.1"
                     /translation="MGPLSIIVASGLLLGIVSILQNPTLVGESFANATVIQGFVGGVQ
                     AIVGTMFGLLPILFAISVAIGMSREDKEIAAFSVVIAFILFHVVINYLLQLRGITAES
                     TSVETLMSDGMSQIKAVETSNMYETLLGTFTYRMGVFGGIIAGLWTAMIHNRYHTKQL
                     PAAFSFFSGNRFVPIMIILTIPIISIASFFIWPFVNTAINALGGLIGKSGVIGTFLYG
                     FSERLLIPTGLHHVLNQLIRFTPIGGTATINGETVSGALTIFNTELALPVKDMDILRS
                     ATRFLTQGTHPFMVFGLPAACYAMYKTSYLAQRPKIKGMLIAAAATCFVTGITEPIEF
                     AFIFISPILWVFHAFMAGLSFMLMNLFGVAVGNAGGGFIDLAIFGILQGTYTRWYLVV
                     ALGLVYAVAYYFVFKYVIERFDVKTPGRSNDDVLEDQDEEMTENELGKLILQGLGGAG
                     NIQEIDNCISRLRLVLKDTATIDEHILKKTGSMGIVKINENNLQVVYGGQVENATRSL
                     KNEMKRK"
     gene            complement(3236..4015)
                     /locus_tag="LP543_002367"
     CDS             complement(3236..4015)
                     /locus_tag="LP543_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL2322504.1"
                     /translation="MTLEQLVNEHHSKLNENDDYVLSFITQNMEISISQTVSELAKQA
                     NVSDSTIIRLTKKLGFKGYGEFRYYLKEEFARMQKTRVDKSSLFLPEVLLGDVQATTK
                     LFEDDKSVEDIYETIRKADKIFAYATGYGQRMMLNEFARCMWNLGIYIIVVPEKNELD
                     LISKALTSNDLLFVISLSGRVDRIDDTLRRIHMKGTQLVSVTRFGQNNLASLSDYSLY
                     YQVTNINDVNELNNSSFCTLNLALSLLYEGYINYYKQSSVD"
     gene            complement(4090..4548)
                     /locus_tag="LP543_002368"
     CDS             complement(4090..4548)
                     /locus_tag="LP543_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL2322505.1"
                     /translation="MGFFSRKEKLVAPVSGNLIPLSQVSDPVFSQGMMGEGFAVEPTN
                     AKVVAPIDGVIKSIFPTKHALTLTSNQGRDLLIHIGIDTVQLKGVGFQVLVEEGQRVS
                     SGESLVIFDCDQIKAEKLSDVVMVLFPEDKGIKLEVSEKKVSEGELLFKI"
     gene            complement(4560..5834)
                     /locus_tag="LP543_002369"
     CDS             complement(4560..5834)
                     /locus_tag="LP543_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBL2322506.1"
                     /translation="MEDLIMKTNFERIKKDIENLAEYNATPGEGVTRSAYSKEDREAR
                     NYLINQMEQLGLEIWEDGFSTLFGRRAGKKADAPSVMIGSHYDSVVNGGRFDGVAGVV
                     TALETMRILEENNFENDYPIDLILMNAEEGETFGPSTGVSNSRAMMGTLTMSELETVK
                     NRHGQTKLEALRAYGVEPDLEAAKRDPKTIKNFIELHIEQGPVLEDEGKDIGLIEFIP
                     GIGRFKARFYGETEDSTAPMNKRKDALVAASKFVLFVEQWMKNLGEGIAGTVGKLTIK
                     PNSNQFVPDFVEALIEIRTFDTKTTNPQEIKEKLLNELSVIQGETGVRTELEEMARIN
                     YSNPTNPSVMDQGNVEKMKHICDQLGYSYQVINNGTGHDAMMMTDFVPTNMIYIPSKG
                     GITHHPDEWTDFEDVKKGADVLLNLVMDISKD"
     gene            complement(5935..6249)
                     /locus_tag="LP543_002370"
     CDS             complement(5935..6249)
                     /locus_tag="LP543_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL2322507.1"
                     /translation="MDYEIQKFISEKEDQYFDRKSARIKPRDIIKHIVAFANADGGKL
                     VIGIEDDGTISGFKFQGAIDPWICVNKVDAFIESFLNQSTLELCYETRKSSYGGIYDN
                     DF"
     gene            complement(6395..7891)
                     /locus_tag="LP543_002371"
     CDS             complement(6395..7891)
                     /locus_tag="LP543_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HBL2322508.1"
                     /translation="MNHQEAKQFFLKTSSFFSTSLPNYIDLSVGIEKAVDLLKNKPFT
                     ELVRHKRSLSEAVDVNHRILANKDGNYAWRPLQILHPLVYVDLVNGITQKNNWNHIKN
                     KFEEFQADKRIECISLPVESISSKSDTAETILNWWENLEQAQISYALEYDYCIHSDIT
                     DCYSSMYTHTIPWAVHSKEWAKTHRKDTQGVGNLIDSKIRYLQNGQTNGIPQGNVLMD
                     FVAEIVLGYADKMLLNKIEKTEIKEFKILRYRDDYRIFSVRKDQAEVIIRLLSEVLSD
                     LNLKLNSNKTFLSDNIILDAIKPDKIYWDLKHASFTEKSNNKWKFKLSLQKHLLQVKL
                     LGDKYPNCGSLSKALSEVYTHRVSTLNKRPDDIYQLISILVNIMQKNPRTLEACIVIL
                     GKLFEFIAVEEINLYLDKILRKFVNTPNTDIVEIWLQRLSLIHSREKSYSAKLCKKVS
                     NPKGFTLWNSDWLKKGFDESGIVNETFISNLSLTTPAKDLELFISQYE"
     gene            complement(8069..8548)
                     /locus_tag="LP543_002372"
     CDS             complement(8069..8548)
                     /locus_tag="LP543_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBL2322509.1"
                     /translation="MKVYTVQPVDFLQSVDSEGFIYPKKRYTELSVFFDNPNAVGALH
                     WLEEEYTKRIQLPLDRDLIWLWRDTCHLKYARKDNFTDFSKYNFYCFEIPIEFYRKNI
                     LWSRFIEWHEPLNHEWETDENQEFEHIFDIPKHPGYGDIQGVTTRLHKDWIKKITHN"
     gene            8715..8864
                     /locus_tag="LP543_002373"
     CDS             8715..8864
                     /locus_tag="LP543_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322510.1"
                     /translation="MRKVDEYIKKKSQKSKVWQRTFELEYKKLSDSLKKDTYNPNEQS
                     KADNQ"
     gene            complement(8924..9169)
                     /locus_tag="LP543_002374"
     CDS             complement(8924..9169)
                     /locus_tag="LP543_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2322511.1"
                     /translation="MLFLKEEKLIDKENVIGSNIRRIRLEKGMGQTELVRELQLSKIA
                     MTRETLVKIEGGRQHIKLDQLRAIRDILQVSYEDLLQ"
     gene            9274..10047
                     /locus_tag="LP543_002375"
     CDS             9274..10047
                     /locus_tag="LP543_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322512.1"
                     /translation="MKKFAIILTCALLTGAISGCATSDTNSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSESNSIEKVLPIEIEKNQENIKLKAELPILNSTEIDW
                     HENIKVTIEKGALAQLYTEKVEQFFESGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMTLKKIKAYDHPLNLTLNNGLLSFVLAGYVDYEYSPDYLETPLQHENNDINFR
                     FVYESKSKTWLVDQLSTINTSFHELPKPENEADMVIKIF"
BASE COUNT         3126 a         2085 c         1561 g         3298 t
ORIGIN      
        1 tcatttcttc cgttttaaag aagaaatgat tttcatagat caacctttaa ctaaagctag
       61 attcttttct aaaaagaatg gtgtgtagta gcccacaccg ctggacaacg gtaaaacttc
      121 atccggcaac tcgatgccca gttcgttacg gatgtactct tggcgagaaa tgattcgcga
      181 ccaaacattt ggatattctt tggctagttg atcacgaagc gactcatcag ctagcgcaat
      241 cccactctca cagcttgcgc cggcatagcc agcaactgat ggaataatat caatttgtag
      301 taacattccg gacttaagag taatttctga gtcttttttc attggcgaag acatccactc
      361 ttcatcagcc gtaagatgcc ccggatttaa gtgccatttg tattccgatt taggcaagac
      421 tttgcctatt actgaatata gctctttacc ttgcattcct attttgatat tttccaacca
      481 tgcgaccacg gcgtcgtagt atggagctgc aactttttca atgtaatcct tttgcccttc
      541 tggtaggtca tctgtcgttg caactgcata gccacttcga cttgctaatc cgcctttaaa
      601 gcccgtagta attgacattt tatcgcctag tttaacttgt ttatcagttg gatatatata
      661 ggcgttctca aaccgttccc cagttgcagc aattggaatg acattatttg gttggccgta
      721 cttaactaag tggttaccaa tttccatttc tgtttttcct acctctactt catcaatagc
      781 gttcatcaca caatctgaag ccaaggatga gccaaattca taatatgcca cttcattagc
      841 agtatttaat gagcgtactc cgtaatcact gtgaatgaac aggtcggtgc gattgctaac
      901 ggcatcttcc cctactaatt ctacaatcga acttacaatg aatgccggca aatcttggat
      961 ttggtagtta tcctcttctg aagtaaacat cttccaacca acaagcccaa ctttcttacc
     1021 tttactgatt ttagcattgg tcaatacttc agttagtggt ttctcgttat ccatcggttg
     1081 atccggtaat gagaagagtg gcgtgtggat taattctgcc tcaattcttg aatagtccac
     1141 cattttagta ttttcatttc ctagaactaa gaaagcttca cccgtctgat gtaataccaa
     1201 caagctttct tcaaagcgag taacaaaacc tgttaaataa gagaagttcc cgccatgttc
     1261 caaatcacaa tagattacga gggtatctat ctctgacttt tccatcgctg caagtacctt
     1321 ttctttacgg aacttcattg tttcatcagt caattgatat attttagctt cattatcttt
     1381 tttaggtggg ttaacttttt ttagagttac cttagtcata ttgtcactac tttctgtttt
     1441 tatttacgct tcatttcatt tttcagagat cgagttgcgt tttccacttg tcctccatat
     1501 accacttgta aattattttc gttaatttta acgattccca ttgagccggt ctttttcaag
     1561 atatgttcat caattgttgc tgtatcttta agaactagtc tcagccgaga gatacagtta
     1621 tcgatttctt gaatattacc agcaccacca agtccttgca agattagttt gccaagttcg
     1681 ttttctgtca tttcttcgtc ctgatcttcc aacacatcat cgttggatcg accaggagtt
     1741 ttcacatcaa atcgctcaat gacatactta aatacaaagt aatatgcaac tgcataaaca
     1801 aggcccaatg ctacaactaa gtaccaacga gtataagtac cttgtaaaat tccgaagata
     1861 gccaaatcaa taaatccacc accggcattc ccaactgcga ctccgaatag attcattaac
     1921 ataaaggata aaccagccat gaatgcatgg aagacccata gaatcggtga gataaagata
     1981 aaggcgaatt caattggctc agtaatcccg gttacaaaac aagtagccgc tgctgcaatc
     2041 aacatccctt taatctttgg acgttgcgcc aaatatgaag ttttgtacat tgcatagcat
     2101 gctgccggca atccgaagac cataaatgga tgagtacctt gtgtcaagaa gcgagtagca
     2161 cttctcaaaa tatccatgtc ttttactggc aatgctaatt cagtattgaa gatggttaat
     2221 gctccagaaa ctgtttcacc attgatagtt gccgtaccgc cgattggtgt aaatcgaatc
     2281 aactggttta gtacatggtg aagacctgtt ggaatcagca ggcgctctga gaagccatat
     2341 aaaaaagttc caattacgcc tgacttacca atcaaaccgc ctaaagcatt gatagctgta
     2401 ttgacaaaag gccagatgaa aaatgaagcg attgagatga ttggaatagt taggatgatc
     2461 ataattggaa caaaacgatt gccactaaag aaactgaaag ctgctggtaa ttgttttgtg
     2521 tgatagcgat tgtggatcat ggctgtccat aatcctgcaa tgataccgcc gaaaacaccc
     2581 atacgatatg tgaatgtgcc aagtaaagtt tcatacatat tggaagtttc tactgctttg
     2641 atttgactca ttccgtcaga catcaatgtt tcaacagaag tgctttccgc tgtgatgcct
     2701 ctaagctgaa gcagataatt gataactaca tggaacagaa taaatgcaat aaccactgaa
     2761 aatgctgcaa tttccttatc ttctctagac atcccgattg ctactgaaat agcaaataga
     2821 ataggaagta gtccaaacat cgtaccaaca atagcctgta ctccaccaac aaatccttgg
     2881 atcacggttg cattagcgaa gctttctccc accaaagtag gattttgtaa aattgaaacg
     2941 attcctagta gtagtcccga tgcgacaata atagacaacg gacccattaa tgatttccct
     3001 aacttctgga tataagttct caatataaac accttcttct ttttagttga tataaacgtt
     3061 tacatccctt gtacaacgaa agtatagcac cctgaaaaag cgctaacaat acgataggtc
     3121 tgataaaaat caacattcag cacttggttg ttcattcaaa acgtgaatct tcaatcacag
     3181 aaaaatacgc gctatcaagc acgtatttta ggtgtaaata tatgcatcaa ctttattaat
     3241 caacgctact ttgtttgtaa tagttaatat acccttcata cagtaagcta agagcaagat
     3301 tcaaggtgca gaatgatgag ttgttcaact cattcacatc attaatgttg gtcacttgat
     3361 agtataggct atagtctgac agactggcta agttattttg accaaatcgt gtaactgaga
     3421 ccaactgtgt ccctttcata tggattcttc ttaaagtatc atcaatccga tcaacacgtc
     3481 cggacaatga tatgacaaat agtaaatcat tcgaggtcaa agcttttgaa atcaggtcta
     3541 attcattctt ttccggtaca acgataatgt agattcccaa gttccacatg cagcgtgcaa
     3601 actcattgag catcattctt tgtccatatc ctgttgcgta ggcaaatatc ttatctgcct
     3661 ttctaatcgt ttcatatata tcctcaacac ttttatcatc ttcaaacaat ttggttgtcg
     3721 cttgaacatc tccgagtaat acttccggta aaaacaaact acttttatcg actctggttt
     3781 tttgcatgcg tgcaaattcc tctttaaggt aatatcgaaa ttcgccatat cctttgaatc
     3841 cgagtttctt agttagtcta ataatcgttg agtcagacac atttgcttgt tttgctaatt
     3901 ccgatacagt ttgactgatg ctgatttcca tattttgagt gataaaactt aatacataat
     3961 catcattctc attcaattta ctgtgatgtt catttactaa ttgctccaat gtcataatca
     4021 gacctccaaa aataataaaa aagattgctc tctcatttta atacgaaagc acaatctttt
     4081 taagtactat taaattttga aaagaagttc tccttcagaa accttttttt cagagacttc
     4141 tagtttaata cccttatctt cgggaaatag aaccataaca acatctgaca gtttttccgc
     4201 cttgatttgg tcacaatcaa aaatcaccaa tgactcgccg gatgatactc gttgaccttc
     4261 ttcaacgagc acttgaaacc cgactccctt taactgaaca gtatcaattc caatatgaat
     4321 caataaatct ctaccttgat tactagtcaa tgtgagtgca tgctttgttg gaaatatcga
     4381 tttaatgaca ccgtcaatcg gtgctaccac cttagcgttt gttggttcaa cagcgaatcc
     4441 ttcgcccatc atcccttgcg aaaatacagg atcgcttact tggctaaggg gaatcagatt
     4501 tcctgatact ggagcaacta atttttcttt tcgactaaaa aatcccattg acttactcct
     4561 taatcttttg aaatgtccat cacaaggttt aaaagaacat ccgccccttt tttgacatcc
     4621 tcaaaatcag tccactcatc aggatgatgt gtaatgccac ctttggatgg aatataaatc
     4681 atatttgttg ggacaaagtc ggtcatcatc atcgcatcat gtccagtacc attgttaatg
     4741 acttgatagc tatagcccaa ctgatcgcaa atatgtttca tcttctcaac attaccttga
     4801 tccataacac ttggatttgt aggattactg tagttaattc gagccatttc ctccagctca
     4861 gttctaactc cagtttcccc ttgaatcact gataactcat tcaacaactt ttctttaatc
     4921 tcttgaggat tagttgtctt tgtatcaaag gttctgattt cgattaaagc ttcgacaaag
     4981 tcagggacaa attgatttga gttcggtttg attgtaagtt taccgacggt tccggcaatc
     5041 ccttcaccaa gattcttcat ccactgttca acaaaaagaa caaatttaga agcagcaact
     5101 aaagcatctt tccgtttatt cattggagca gtcgagtcct cagtttctcc ataaaaacgt
     5161 gctttaaaac ggccgatgcc aggaataaat tcaatcaatc cgatatcttt gccttcatct
     5221 tccaggactg gtccttgttc gatatgaagt tcaataaaat ttttgattgt ttttggatct
     5281 cgcttagcgg cctctaaatc tggttccact ccataagcac gaagagcttc caattttgtt
     5341 tgtccatggc gattttttac tgtttccaac tcactcatgg ttaatgttcc catcatagct
     5401 cgtgaatttg agacacccgt acttggccca aatgtctcgc cctcctctgc attcatcaga
     5461 attaggtcaa ttgggtaatc attttcaaaa ttattttctt ccagaatccg cattgtttcc
     5521 agtgcagtaa caacgccagc cactccgtca aatctgcctc cattaacaac tgaatcataa
     5581 tgagagccaa tcatcactga tggtgcatca gcctttttac cggctctacg accaaataat
     5641 gtagagaagc cgtcttccca aatctctaaa cccagttgtt ccatttgatt gatcaaataa
     5701 tttcttgctt cacgatcttc tttagagtaa gcagagcggg tcactccttc acctggagtt
     5761 gcattgtact cggctaaatt ttcgatatcc ttttttatac gctcaaaatt tgtcttcata
     5821 attaaatcct ccatttccta caagtgaatg ttagcaccca tagaaagcgg ctacaatata
     5881 atcaatgaag attgaatcga tattcgaaaa ctgaatattg cttcggttaa tgacttagaa
     5941 atcattatcg tagattccac cataagaact ttttctcgtc tcataacaca actcgagagt
     6001 ggattggttt aaaaaactct cgataaatgc gtccacttta ttgacacaga tccatggatc
     6061 gatcgcacct tgaaatttaa acccagaaat agtcccatca tcttctatac cgattaccag
     6121 cttacctcca tcggcatttg caaacgctac tatgtgtttt ataatatctc taggcttaat
     6181 ccgtgcactt tttctatcaa aatactgatc ttctttttca cttataaact tttgaatctc
     6241 ataatccatt taaaatttcc cttcttaaat ttttttatta tagagccgta tattcaattt
     6301 ccacaaaaaa ataatctaaa acaaaacaga caccttcatt ttgatatgtt ctcccacata
     6361 tgaatcggtc gctatacagc attacatata gtaattattc atactgcgaa ataaatagct
     6421 ccaaatcttt agctggcgta gttaagctta aattcgaaat gaaagtttca ttgactattc
     6481 cactttcatc aaatcccttc ttcaaccaat cagagttcca caacgtaaat cctttaggat
     6541 tagaaacttt cttacataat tttgcactat aggatttctc tcttgaatga attaaagaaa
     6601 gtctttgtaa ccaaatttca actatatccg tattaggagt attaacaaat tttcttaaga
     6661 ttttatccaa gtataagttg atttcttcta cagcaataaa ctcaaaaagc ttacctaaaa
     6721 tcacaataca agcctccaaa gttctaggat tcttttgcat tatattaaca agaatactaa
     6781 tcaattggta gatatcatct ggtcttttgt ttaaagtaga aactctatgt gtatagactt
     6841 cacttagcgc tttacttaaa ctcccgcaat ttggatattt gtctcctaaa agctttacct
     6901 gtagcaaatg cttttgaagg ctaagcttaa acttccactt attattactt ttctctgtaa
     6961 aacttgcatg tttaagatcc cagtaaattt tatcaggctt aattgcatct aaaattatat
     7021 tatccgataa gaatgtttta tttgaattta gcttcaaatt tagatccgat aatacttcag
     7081 ataacaatct gataatcact tcagcttgat cttttcgaac cgaaaaaatt ctgtaatcat
     7141 ctctgtaacg aagtattttg aactctttaa tctctgtttt ttctatcttg ttcagtaaca
     7201 ttttatcagc atacccaagt actatttcag ctacaaaatc catcaataca tttccttgag
     7261 gtataccgtt cgtttgtcca ttttgtaaat atcgtatttt agaatctatt aagtttccaa
     7321 cgccttgagt atcttttcta tgtgtcttag cccattcttt tgaatgtact gcccacggga
     7381 tagtatgtgt gtacattgag ctataacaat cggtaatatc tgaatgaatg cagtagtcat
     7441 actccaaagc ataactaatc tgtgcttgtt caaggttctc ccaccaattt aaaatagttt
     7501 ccgcagtatc gctctttgaa gaaatagatt ccacaggaag tgatatacat tcaattcgct
     7561 tgtcggcttg gaattcttca aacttatttt tgatgtgatt ccaattattt ttctgtgtaa
     7621 tcccgtttac taagtctaca tatactaatg gatgtaatat ttgaaggggt ctccaagcat
     7681 aattaccatc tttattagct aatattcgat gattaacatc aactgcctca gagagcgatc
     7741 tcttatgtct aactagttca gtaaatggtt tgttttttaa taaatccact gctttctcaa
     7801 tgcctactga taaatcaata tagttgggca acgatgtact aaaaaaactt gatgttttta
     7861 aaaagaattg ctttgcttct tgatgattca tctctaaaat actttttcta gtcctagtca
     7921 aattcaaaat ctcctagatc aattaagttg taatgtaaga ggatacccta aggttacaca
     7981 ccctatttac tattatacca gtattcattc gtgatacata agggtgtaac attaagaact
     8041 ctatctaaaa gactacaagt agtcaccttt aattgtgagt aattttcttt atccaatcct
     8101 tgtgcaacct tgtcgtcacc ccttgtatat ccccgtaccc cggatgtttc ggaatatcaa
     8161 aaatgtgctc aaattcctga ttttcatcag tctcccactc atgattcaac ggttcatgcc
     8221 actctataaa ccttgaccat aaaatgttct ttctataaaa ctcaatagga atttcaaaac
     8281 agtaaaaatt gtatttgcta aaatctgtaa aattatcttt acgagcatac tttaaatggc
     8341 aggtatctcg ccacaaccaa attaaatctc gatccaatgg taactgaatt cgtttcgtat
     8401 actcttcttc aagccaatga agtgcaccaa ctgcattcgg attatcgaaa aagacagata
     8461 attcagtata gcgtttcttt ggataaataa atccttctga atcaacagat tgcaaaaaat
     8521 ctactggttg aaccgtgtac actttcattt attcgccacc tttttttgac tacttatagt
     8581 catttaccaa tttgaaatct gcatactttt tcattcaatt gagcccagca ttaattgtac
     8641 acgagttttc ttcactaacg gatagtatca ggatatacta atactatcct gaatagcgaa
     8701 atgaggtagc caaaatgaga aaagtcgatg aatatatcaa aaagaaatcc caaaaaagta
     8761 aagtatggca aagaacattt gagttagagt ataagaaact ctcagatagc ctcaaaaagg
     8821 acacttataa tccaaatgaa caaagtaaag cagataacca ataaagatta ccctcccata
     8881 tttaacattg cttcacgaat cagtaatcaa tgactacaag tagtcattga agcaaatctt
     8941 cataagatac ctgtagaata tccctaatag ctctcaattg atccagctta atatgttgcc
     9001 ggccaccttc aatcttaacc aatgtttctc ttgtcatagc gattttactg agctggagtt
     9061 ctcgcaccag ttctgtctgt cccattccct tttctaatcg gattcgtctg atattgctac
     9121 cgattacatt ttctttatct attaactttt cttccttcaa aaatagcacg ccttttggac
     9181 taaaattagt cctttttctt attaatctta aagatttggt atgctacaat gggactagta
     9241 ttagtccata ttcaatttta aggaggaatt tttttgaaga aatttgcaat cattctaacc
     9301 tgtgctttac tcacgggagc tataagtggg tgcgccactt ctgacacaaa ttctacaagt
     9361 actgaaacaa gtacaacaac ctcagaaaag aattatccta tacatttttt tccagttcca
     9421 gatggatttg aagcttatta tcatgataaa aaactagatg ttccattgtc agaaagtaac
     9481 tctattgaaa aagttttgcc aattgaaatt gaaaaaaatc aagagaatat aaaactcaag
     9541 gccgagcttc ccatactgaa cagcacagaa attgattggc acgagaatat taaagtaact
     9601 attgaaaaag gagcgttagc acaactttat actgaaaaag ttgaacaatt ttttgagtct
     9661 ggtgccaata aagacttttc aaaaataagc gattattcgg ataacttttt taatgagacc
     9721 caagatgaaa tcacgaactc aacttttatg gctgaagata tgtacaatat gacgttaaaa
     9781 aaaataaaag cttacgacca tccattaaat ctgacactaa ataatggatt actctctttt
     9841 gtattagcag gttacgttga ctatgaatat tcaccagatt atttggagac acctcttcaa
     9901 cacgagaaca atgatattaa ttttagattt gtctacgagt caaaaagtaa aacatggtta
     9961 gttgatcaat taagcactat aaacacaagc ttccatgaat tacctaaacc tgaaaatgaa
    10021 gctgatatgg tcattaaaat cttctaaaaa agaagcgttg aaggagaaat
//
LOCUS       DAFEPQ010000085         9871 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.087, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000085
VERSION     DAFEPQ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.087"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            11..538
                     /locus_tag="LP543_002376"
     CDS             11..538
                     /locus_tag="LP543_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBL2322513.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            601..1971
                     /gene="radA"
                     /locus_tag="LP543_002377"
     CDS             601..1971
                     /gene="radA"
                     /locus_tag="LP543_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBL2322514.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            2005..3162
                     /locus_tag="LP543_002378"
     CDS             2005..3162
                     /locus_tag="LP543_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBL2322515.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            3332..4792
                     /locus_tag="LP543_002379"
     CDS             3332..4792
                     /locus_tag="LP543_002379"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBL2322516.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     misc_binding    4789..5028
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5114..5650
                     /gene="cysE"
                     /locus_tag="LP543_002380"
     CDS             5114..5650
                     /gene="cysE"
                     /locus_tag="LP543_002380"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBL2322517.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            5647..7059
                     /locus_tag="LP543_002381"
     CDS             5647..7059
                     /locus_tag="LP543_002381"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBL2322518.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            7056..7451
                     /locus_tag="LP543_002382"
     CDS             7056..7451
                     /locus_tag="LP543_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBL2322519.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            7444..8304
                     /gene="rlmB"
                     /locus_tag="LP543_002383"
     CDS             7444..8304
                     /gene="rlmB"
                     /locus_tag="LP543_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBL2322520.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            8308..8838
                     /locus_tag="LP543_002384"
     CDS             8308..8838
                     /locus_tag="LP543_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBL2322521.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            8915..9493
                     /locus_tag="LP543_002385"
     CDS             8915..9493
                     /locus_tag="LP543_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL2322522.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            9606..9851
                     /locus_tag="LP543_002386"
     CDS             9606..9851
                     /locus_tag="LP543_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBL2322523.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT         3367 a         1784 c         2214 g         2506 t
ORIGIN      
        1 aggaaaaccg gtgagaaaaa gaggatttga agttatttct aaatataaag atcaaaatat
       61 caatctaccg cagcgcgcta cacatcatgc tgctggctat gattttgaag cagcagaaga
      121 ggtcgtgatt ccaagtatct ggaaagcagg ctggaaaaag gggttggaaa cagtcgcaga
      181 tggattgaaa aaagatgtat cagaaaatga aaaacaattg aaaccaaccc tcgtgcctac
      241 tggaatcaaa gcttacatgg gagaagatga atttttgcag cttgctaacc gttcaagcaa
      301 tccgttgaag cggtttttag tgttgacaaa cggagtaggt gtgattgaca gtgattatta
      361 caataatgag gcaaatgaag gccatatcat gttccaattc accaactttg gtttcaagga
      421 tgtagtgatc aaaaaaggcg agcgtatcgg ccaagggatc tttttgccgt ttttaaaagc
      481 agatcaagat caaacgactg ctcaacgtca aggcggattt ggttcttcca gtaaataaaa
      541 cgttaaagat acttaagctt gatttaatgt ctcatgatta ggataaaagg aggcggtaca
      601 atggcaaaaa aatcaaaagt acaatttgtt tgccaaaatt gcgggtatgt gtcgcctaag
      661 ttcttgggac gctgcccaaa ttgcggaaaa tggaatacga tggtcgaaga aatcgaacaa
      721 gatacaacgg accgcagaac tcggacaagt ttgacaggag aaaaagcaaa accaacaaaa
      781 atcgcagacg tcgtccctaa aaaagaacct cggatcaaaa caaaattaga agaactgaac
      841 cgtgtgttag gcggaggtgt ggtgccaggg tctatggtct tgattggtgg cgatccaggt
      901 atcgggaaat ccactttgct gttacaagtt tctcagcaat tagcagccat aggcggaaaa
      961 gtcttatatg tatccggcga agaaagcgca gagcagataa aattacgcgc ggaacgcctc
     1021 ggaagtatca acacagaatt ttatctatac gctgaaacag atatgaacga aatcagtcgt
     1081 gccatcgaac atatctcccc tgattatgtt atcattgatt ccatccaaac gatgacgcag
     1141 ccagacatca caagtgtggc aggaagtgtc agccaagtaa gagaaaccac tgcagaacta
     1201 ctcaaaatcg ctaaaacaaa tgggatagcc atttttatcg tcggtcatgt taccaaagaa
     1261 ggctcgatcg ccggcccaag aatgttagaa catatggtgg atacggtgtt atattttgaa
     1321 ggtgaacaac atcatagttt tcggattttg cgagcagtaa aaaaccgatt tggctcaacg
     1381 aatgagattg ggatttttga aatgcatgag catggattgg aagaagtcgc taatccttca
     1441 cagattttct tagaagaacg cctagacggt gcaactggct ctgctatcgt tgtggcaatg
     1501 gaagggacac gtccaatttt ggtagagatc caagcattag tcacacctac tatgttcgga
     1561 aatgccaaac gaacaacaac aggtcttgat ttcaatcggg tatctctgat catggcagtg
     1621 ctggaaaaaa gagctgggtt gcttcttcag aatcaagatg cttacttaaa agctgcaggt
     1681 ggggtaaaat taaatgaacc agcgattgat ttagctatcg ctgtcagtat tgcttcaagc
     1741 tataaagaaa aaggaacaca gccaacggaa tgtttcatcg gtgaaattgg actgactgga
     1801 gaaatcagac gtgtcagtca tattgaacaa cgagtcaaag aagtccaaaa actcggcttc
     1861 acaaaagtat acctacctaa aaacaactta ggtaactggg aagcgccaaa aggaatcgag
     1921 atagttggag tagcaacttt ggctgaaacg ttgaagcgtg tgttccgcta actaataaat
     1981 gattgtcgaa acggaggaaa aacgatgcaa aaacgtgtca ttactttgct gatgatcatt
     2041 gtcggcgcaa gcttgggtat ttcattttta ccctcagctt ggcaggcaat cggtcaacag
     2101 tcaaacggat ggctgaataa tacatttacc aacagcctgt taggcgcact tattttcttt
     2161 ttattatctt tgctgttggc caaacatatc tcagctatta tcaaacaagt agaacaaaaa
     2221 ctaagtgagg tcagcttgac ttatcttctc ttcggtgcga tcggtgcgat catcggattg
     2281 actttaggtg ttgtgatctc gacccctctt tacaatatgg aaattccatt cgtcaatagt
     2341 gtattgccga ttttactgat gatcatctta ggctatctgg gattgagaat gggaacgaca
     2401 cgaatcgaag aatggaagaa agtttttggt tcacgaagca aaaaaatcga gcaagaaaca
     2461 gctagtcaag ttgagagtca actgttagaa cgaaaatcag gagaaaattt ccataaatat
     2521 aaaatcttgg atacaagcgt gatcattgac ggtcggattt atgatattac aaagactgga
     2581 ttcttagaag gaaccttgat gatccctaat tttgttcttt acgaactaca atacatcgcg
     2641 gactcagggg atagtttgaa acgtgtgcgt ggtcgtcgcg gattagatat cctaaatgcc
     2701 ttgcaaaaag aagagggtat atctgtcgaa atgtacgatg gagactttga agatatcagt
     2761 gaagtagaca gcaaattgat caaattggct aagcttttag atggtgtcat tgtaaccaat
     2821 gactataact tgaataaagt ctctgaattc caaaatgtac ctgttcttaa tatcaatgcg
     2881 ttggcaaatg ccgtaaaacc agtcgtcatc cctggtgaaa cgatgaatgt tctagtagta
     2941 aaagccggga cagaacgcca acaaggagta gcctaccttg atgacggcac gatggttgtc
     3001 gtagaagatg gacagcatta catgaatgaa cgtatcgaag tcgttgtaac aagtgccctt
     3061 caaacagctg ctggacgcat gatctttgct aaacctgcac atgcaggacg aggaatcgac
     3121 aataaaaaag agacagagaa aaagactaaa caatcaaaat aaaaaagaaa ttcagccaac
     3181 tggtaaggaa attcaaccag ttggctttac tatttacctt agaaaaggta aaatttgtgc
     3241 tgagtggctt taaataaatg atatttgaga agccgtaact gacggaattt ttgctgttag
     3301 tcaaagtcta gtttgaaaga agaggaagaa aatgacgaaa gtacgcgtac gctatgcacc
     3361 aagtccaact gggcacctgc atattggaaa tgcaagaact gcattattca attatttgtt
     3421 tgcccgccac aatgatggag actttattat tcgtattgag gatactgacc aaaaacgtaa
     3481 tatcgaagat ggcgaaaaaa gccagttgga aaaccttgct tggttgggaa tgaattggga
     3541 tgaatctcct gaaaatcctg gagaatacgg cccttaccgt caatctgaac gtaaagaaat
     3601 ctatcaaccg ctgattgatc agctgcttgc tagcaatcga gcatacaaat gctattgcac
     3661 agaagaagaa ttagaagcag aacgggaagc acaacgcgca agaggagaaa tgcctcatta
     3721 cgcaggaaca tgtgcgaact tgacacctga agaacaagca gaaaaagaag cacaaggtct
     3781 agaatctgtt gtacgtttcc gtgtgcctcg caatacagaa tacgcattta cagatatggt
     3841 gaaaggcgct atttcgttcg aatccgataa tatcggtggc gactttgtca tacaaaaacg
     3901 cgatggtatg ccaacttata attttgctgt tgcggtcgat gatcatttga tgaaaatcac
     3961 tcacgtttta cgtggagatg accatattgc caatacacca aaacaactga tgatctatga
     4021 agcatttggc tggacaccac caaccttcgg acatatgaca ttgatcatca acagtgaaac
     4081 aggtaaaaaa ctaagcaaac gtgacgaatc aatcttgcaa tttatcgaac aatatcgcga
     4141 attaggctat ctaccagatg caatgttcaa ctttattgca ttattgggct ggtcacctgt
     4201 gggcgaagac gaaatcttcg aacaccaaga attgatcaaa atgttcgatc caaaacgcct
     4261 aagtaaatca cccgcagctt tcgatgcgaa aaaactgcaa tgggtaaaca accattacat
     4321 caaagcaatg gatctagatg catttactga catgtgtctg ccttatctaa tcgctgatgg
     4381 acgtgtagca gaaaacccag atgaaaagac aatcgaatgg gtgaagaaga tcgttagcct
     4441 gtatcaacct caaatgagct atgctgcaga aatcgttgat ctgtctacgc tatttttcaa
     4501 tgaacatcct gtattgaatg aagcagcaaa agaagtactt gctggtgaaa cagttcctac
     4561 tgttctccac gcattcaaag cacagctaga acaaatggaa gtagtagatg ctccaactgt
     4621 caaagctgcc atcaaagctg ttcaaaaaga aactggcgta aaaggaaaaa atcttttcat
     4681 gccaatccgt gtagctgtat caggacaaat gcatggtcca gaattgcctg aaacgatcga
     4741 attgcttggt cgtgaaaaat cattagcaca tttaaatcaa gtactgaact aaacacgatg
     4801 aacagaaaaa agtaagcagc tgatcattac aagagagata ccgggtggtg caaggtatca
     4861 atgaatggtt gccgaaatgc ctctgggagt ggtcttggcg aaacacagaa aacaggaaaa
     4921 actgttttat aggaaacaaa gtagccaaga acgtataaca ggcgttagct gtcagagata
     4981 atcattgatt atgaagaaaa gtggaaccac gcggaaagcg tcttttgata aggaaactta
     5041 tttaaaaggc gcttcttttc tttataagaa gatgatttcc tacataaaat tcttacgaaa
     5101 ggagtggaaa gtcatgagtt ggtttacccg agcgattgat gcagcaaaga gaaacgatcc
     5161 ggctgcacgt tcgacagctg agatcgtttt gacataccct ggttttcatg ccttgttttg
     5221 gcatcgtttc tctcattttt tatatcagca ccggctcttt ttattggcga agatcaatgc
     5281 tcaattctgg cgtttcctaa ctggtgttga gatccatcca ggagcaacga ttggaactgg
     5341 cgtgttcatc gaccatggaa tgggaatcgt cattggcgaa accgcagaaa tcgaggacga
     5401 tgtgatttta ttccatggcg taacattagg cggcacagga aaagaaacgg gtaaaagaca
     5461 cccaactgtt aaacaaggag ccatgctctc tgcgaatgcg cagatattag gaccggtaac
     5521 gatcggaaaa aacgccaaaa tcggtgcagg tgccgttgtg ttgaaagatg ttccagacga
     5581 cgctacagcg gtaggagtac cagcaaaagt cgttcgaatc aaaggagaaa aagtgaggag
     5641 atcagaatga taaaaatcta caatacattg accagaaaaa aagaagaatt catccctata
     5701 caaaagggac atgtcggtat gtatgtctgc ggaccgactg tgtataatta tatccatatc
     5761 ggaaatgctc gtagcaccat tgctttcgat acaattcgac gctattttga atatcgaggc
     5821 tataaagtcg atttcgtatc gaatttcacg gatgtagacg ataaaatcat ccgtacagca
     5881 aacgaattag gtattacagc accagaagtt gctgatcgtt tcatccgagc atttgaagaa
     5941 gatacgaaag cgttgaatgt cgagcctgct acactgcatc caagagtcat ggatcatatg
     6001 ccagatattt tagcatttat tcaagtgttg gttgaaaaag gctatgctta tgaatcttca
     6061 ggtgatgtgt attaccgtac ccgtaaattt gaaaattatg ggaaattaag tgaccaatcc
     6121 atcgatgaac tggaagtcgg agcaagtcag cgtacagggg aagaacagca gcaaaaagaa
     6181 gacccattag atttcgcttt atggaaacaa gcaaaacctg gcgagatttc ttgggattca
     6241 ccatggggca aaggacgtcc cggttggcat attgaatgtt ccgttatggc aacaaaacat
     6301 ttaggagaaa cgatcgatat ccatggtggc gggcaagatt tagaatttcc tcatcatgaa
     6361 aatgaaatcg cacaaagtga agcgaaaaca gggaaaaaat tcgctaatta ctggatgcac
     6421 aatggctatg taacgattgg cgaagacgat gaaaaaatga gtaaatctct agggaatttc
     6481 atcactgtac atgaaatgat caaaaacgtt gatccacaag tcttgcgttt cttcatggcg
     6541 accacacaat accgtcgtcc gatccgctac agtgaaacga cgatgaaaga agcagccacc
     6601 aatttacaac gactgaagaa tacgtttgac aatttgaaat tccgccaaga aactgcctca
     6661 gagaatttgg cagatgatgc ggataaaatc gctttgttag aaaatttaga acagcgattc
     6721 atcgaagaaa tggacgatga ttttaatgca gcaaatggga tcacagtggt ctatgagctt
     6781 gctaagtggc tgaacactta tctagaccag ccgaaagttt ctgccgctgt cttggcacaa
     6841 ggagaagctt tattctcgca atggctttcg atcttcggta ttttatttac agcagaagag
     6901 ttattagatg aagagattga acaattgatc gaagaacgaa atcaagcaag aagcaatcgt
     6961 gattttgtcc gaagcgacga aattcgtgat ttgttgaaag aaaaagggat cttattagaa
     7021 gatacaccgc aaggaacccg atggagaaga gaagcatgag agattacaca caattgaacg
     7081 gtttagcctt ggcttatgtt ggagatgcga tttacgaaat ctatgttcgt gaccacctga
     7141 ttgatcaagg agaaacaaaa cccaataagc tgcatcgtgc ggcaacgaat tatgtttctg
     7201 cgaatgcaca agcctttttg attcagcaaa tgctggaaga acatcttttg acagaagaag
     7261 aagaacttta ttatcgcaga ggaagaaatg ctaaaagcca tacgagcgca aaaaatgctg
     7321 atgttacgac gtatcgggtc gcaacagggt tcgaagcgct catgggctat ctgcatttga
     7381 caaaacaaac cgagcggatg gaagaattga tccgctggtg tattcagaaa gttggtgaaa
     7441 aaaatgggta aagaaagaac aaacaaacat caatcgaaac gtggatttga agacaaaaaa
     7501 caaaatagat attcaaataa aaagcaaact ggacgaaatc agtcagtttc tgatcaaaat
     7561 gaagaagcat tagaagataa tttcgtcttt gggcaccatg ccgcagtaga agcgctacaa
     7621 caagggcgag ggaataaact ctttcttcaa gaagattcac gaggagaaaa agtagaagaa
     7681 ctgaaagaaa tcgcacgtga acaagctgtg ccagtcaaat gggttcctaa acaaaaatta
     7741 gaaactttga gcgatcacgg cgtgcatcaa ggagtcgttc ttgcgattac accttatgaa
     7801 tatttatccc ttgaccaact gattgaacga acaaaagaga aaaaagaaga ccccttcttt
     7861 ttgattttgg atagcatcga agatccacac aattttgggt cgattttacg gacagcagac
     7921 gcaagtggcg tcgatggtgt gatcatacca aaacaccgag cagttggaat cacacctatc
     7981 gtaacaaaga cttccactgg ggcagtcgaa catgtccctg ttgcacgcgt gacgaatttg
     8041 gtacaagctg tgcaacagct caaagaaaca ggattttggg tatttggcac agatatgacc
     8101 ggcacaaact tccaaaaatg gaatgccaaa ggatcaattg ctttagtcat tggtaatgaa
     8161 ggcaaaggaa tgagtccagg attgaaaaaa gaaatggatg aattgctgac gatcccgatg
     8221 accgggcacg tccagagctt aaatgccagt gttgctgctg gtttgcttat gtatcaggct
     8281 ttttcccaac gtcaggggga gtagaccatg aaaaaacagc tgttgattgt agacggctac
     8341 aacatgattg gttcgtggcc ggaacttgtc cagcttaaaa agcaggacaa aatggcggat
     8401 gcaagagaag ctcttttaca tcgtttatca aattatgcga aatatgaagg aatggaagtc
     8461 atcgttgtct tcgatgccca actagttccg ggcatccaac aaaattataa gaaataccag
     8521 ttggatgttg tttttacgaa agaagacgaa acggctgaca gttatatcga gagaatcgct
     8581 ggtcagctga atgaccgttt gacacaagtg acagtggcga caagcgattt agcagaacag
     8641 tggatgatct tttcccaagg agcattacga acttctgctt atgaattata taagacgata
     8701 aaaaaaacgg aaaaaacaat agcccaagaa aataaagata tgcattttat tgatttccga
     8761 cgtaattctc catggaatga agagcagtta aaaaaattgt cccaaaaatt ggacgattta
     8821 atgagaaaaa ataaataaca tatcgttcta atcagttcga tgaatcattg atgataagat
     8881 tcctccataa acgaatttat ggaggaatta ttttatgtct gaggagttat tgcgcgctgc
     8941 acgtaatggt gacgaagaag catttgctgc cctgtatcaa aaatattttc cacttttact
     9001 acgtttgaaa aacacctatt atttaagaaa ttatgtttct gaggattggg aacaagaagc
     9061 aaggatcgct ttatatcagg cattgagaac ttacgaaaaa agcaaaggtg ttagctttgg
     9121 aagttattat aaaagcatta taagcaatca aatctattct ctgttgagga agcagaacgc
     9181 gctgaagcgt cgtgatcaaa aagatgaagt ctcaatcgac caaaaaatag agacagaagg
     9241 accagacttt ttagcagaga tcacattgca agaaccttta gcgattcagc atcttttatt
     9301 aaaagaagca atcgaaaatt gcgaaatcca gttttcaaaa atagaagcta aaatttttca
     9361 ttattatatg caaggcgttg attttgtaga catggctgaa tgtttagcaa tggatcacaa
     9421 gcaagtaatg agcgcttacg ggagagtaaa actcaaaata aaaaaacact tgaaatttta
     9481 taatgaggaa taatttagaa aaatcttaat gagcttctta tttattttta ttgcatttct
     9541 tttttttatg gtaaacttga aaattttaaa aatatgtggt atagttgata gtagaggtga
     9601 actgtatgcc aacaacttta gcttcaatca agaaagattt agaaggtcgt atcggcagcc
     9661 caatcatgtt ggttgctcag acaggaagaa agcgtcaaac agaacgccgc ggcgttttaa
     9721 cagaaaccta tccttcagta ttcgttgttg atttggatcc tgaagaaaat tcttttgaac
     9781 gagtatctta cagctattca gatgtactta ctcggacagt tgagatcgaa tttttaactg
     9841 aagcagttta aaaagcaaac gagcctgaga t
//
LOCUS       DAFEPQ010000086         8987 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.088, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000086
VERSION     DAFEPQ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.088"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..1023
                     /locus_tag="LP543_002387"
     CDS             <1..1023
                     /locus_tag="LP543_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBL2322524.1"
                     /translation="ELKPKHERQNFLVGDDRLDHAVAFLWKDPQTGETVGASYQGTIV
                     DFNRFGKRGTYKHIDKNPTPNHGFNLKIGDPKHLKFFESSIDLLSYAALNREKLQDAW
                     LVSMDGLKHHVISHYVEESISELRRKQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDP
                     FTNKKIRCERGIPNDWQVPKEYKATYEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVS
                     AHDVQSTFGKMLAKGEPVETIDLKEACTTVAKELKVCERADGTYNFDRFYSRKANIKD
                     VNAGILLSYKAEQYYKGYKKHEHEFVPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQG
                     RQQERE"
     gene            1034..3043
                     /gene="topB"
                     /locus_tag="LP543_002388"
     CDS             1034..3043
                     /gene="topB"
                     /locus_tag="LP543_002388"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL2322525.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            3159..3308
                     /locus_tag="LP543_002389"
     CDS             3159..3308
                     /locus_tag="LP543_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322526.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            3389..3985
                     /locus_tag="LP543_002390"
     CDS             3389..3985
                     /locus_tag="LP543_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBL2322527.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPEKTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            3998..4897
                     /locus_tag="LP543_002391"
     CDS             3998..4897
                     /locus_tag="LP543_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBL2322528.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            4900..5031
                     /gene="ssb"
                     /locus_tag="LP543_002392"
     CDS             4900..5031
                     /gene="ssb"
                     /locus_tag="LP543_002392"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL2322529.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            5053..5385
                     /gene="ssb"
                     /locus_tag="LP543_002393"
     CDS             5053..5385
                     /gene="ssb"
                     /locus_tag="LP543_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL2322530.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            5430..5663
                     /locus_tag="LP543_002394"
     CDS             5430..5663
                     /locus_tag="LP543_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322531.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(5822..5944)
                     /locus_tag="LP543_002395"
     CDS             complement(5822..5944)
                     /locus_tag="LP543_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HBL2322532.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            6801..7007
                     /locus_tag="LP543_002396"
     CDS             6801..7007
                     /locus_tag="LP543_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBL2322533.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            7007..7258
                     /locus_tag="LP543_002397"
     CDS             7007..7258
                     /locus_tag="LP543_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322534.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            7274..7429
                     /locus_tag="LP543_002398"
     CDS             7274..7429
                     /locus_tag="LP543_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322535.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIE"
     gene            7439..7711
                     /locus_tag="LP543_002399"
     CDS             7439..7711
                     /locus_tag="LP543_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322536.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            8156..8335
                     /locus_tag="LP543_002400"
     CDS             8156..8335
                     /locus_tag="LP543_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322537.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            8394..8750
                     /locus_tag="LP543_002401"
     CDS             8394..8750
                     /locus_tag="LP543_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322538.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         3230 a         1526 c         1853 g         2378 t
ORIGIN      
        1 gaactaaagc cgaaacatga acggcaaaat tttttagttg gagacgaccg tttagatcat
       61 gcggttgctt ttttatggaa agatccacag acaggagaga cagtaggggc aagttatcaa
      121 ggaacgatcg tcgattttaa tcgtttcggt aaacggggaa cgtataaaca cattgacaag
      181 aatcctacgc caaatcacgg atttaattta aaaataggcg atccgaagca tttaaaattc
      241 tttgaaagta gtattgatct gttaagttac gctgcattga atcgggagaa actacaagat
      301 gcctggttag tttcgatgga cgggttaaaa catcatgtaa tcagtcatta cgttgaagag
      361 tcgatttctg aattaagacg aaaacaaaca tttcctcaat cgattgaaat atgtgtggac
      421 aatgatcgtg caggacatat tttttatgaa aaagaacaga tgaagggaat cgttgatcct
      481 tttaccaata agaaaatccg ttgtgagcga ggaattccaa atgattggca agtacctaaa
      541 gaatacaaag ctacttatga agcagtcgca aaagaaatga gtgttgaacc tgaagcaatt
      601 atggccatac acaaaactga aactaattta cagctgacaa atcaattggt gtctgctcat
      661 gatgttcaat ctacgtttgg aaagatgttg gcgaagggcg aaccagtaga aacaattgat
      721 ttgaaagaag catgtaccac ggtcgcgaag gaactgaaag tatgtgaacg cgcagatgga
      781 acatacaact ttgatcgttt ttatagtaga aaagccaaca taaaagacgt taatgcagga
      841 atccttcttt cctataaagc agaacaatac tacaaaggct ataaaaagca tgaacatgaa
      901 tttgtacctg aagtgaaaaa ggattggaat gatcaattga agcatgagat ccagcaacaa
      961 gaaatcagaa agcaaaaacg tgcgatgttg tttcaacaag gcagacaaca agaaagggag
     1021 tgaagtcgtc actatgaaaa ccgtcatttt agcagaaaaa ccgtcacaag cgaaggctta
     1081 tgccgatagc ttttctaaag ctacccgaaa agatgggtat tttgagatcc aagatcgatt
     1141 gtttacagga gaaactgtga tcacgtatgg ttttggtcat ttagtcgagt tagattcgcc
     1201 cgatatgtac gatgaaaaat ggaaacaatg ggcattagaa catttgccta ttttccccaa
     1261 tcaataccac tatcatgttc ctaaagataa gaaaaagcag tttagtgtcg tcaaacgaca
     1321 gctgcagtca gctgatacga ttgtgatcgc gactgatagc gatcgtgaag gggagctgat
     1381 tgcctggtca atcattcaac aagcaggcgc caatcaagga aaaaccttta aaagactttg
     1441 gatcaactcc ttagaaaaag aagccattta tcagggattc caacagttac gagacgcaga
     1501 agagacctat ccaaaatttg aagaagtaca agcacgtcag atcgccgatt ggttaattgg
     1561 gatgaacggt agcccactgt atagtttact cttgcagcaa aaaggcatac caggaagttt
     1621 ttctttaggt agagtccaaa ccccaacact ctatatgatt tatcaattgc aggaaaagat
     1681 tagaaacttc caaaaagaac catattttga aggaaaagcc caagtaatcg ctcaaaatgg
     1741 cgcatttgat gcgaagcttg atccaaatga aacccaagcg acacaagaag catttgaagc
     1801 ctatttaaaa gaaaaaggtg ttcaattagg aaaacagcca ggtacgatcc ttcaagtcga
     1861 aacagagaaa aaaagtgcgg ctagcccacg tcttttttct ttatcgagcc ttcagtctaa
     1921 aatgaatcaa ctcatgaaag ctagtgccaa agatactttg gaagccatgc aagggttgta
     1981 tgaaggaaaa tatctaagct atccgcgaac ggatacccct tatattacgg aaggggaata
     2041 tgcgtatctg ttggatcact tagacgaata caagcatttt ttaaaagccg aagcgattcc
     2101 gactcctatt catacaccga atagtcggta tgtgaataat aagaaagtcc aggaacatta
     2161 cgcgatcatt cctacaaaaa cggttatgac agcagcggct tttgaacaac taagtccact
     2221 gcagcaggct atttatgaac aagtgttaaa aacaacggtg gccatgtttg cggaaaagta
     2281 tacctatgaa gaaacaacga tcttgacgca agttcagcag cttcagttaa aggcaatcga
     2341 aaaggtgccg ctagatctag gttggaagaa gttatttgga aaagagagcg acggaaaaga
     2401 gaaggaagaa gagccgctac ttcctaaagt gacaaaagga gaaatggtga cggtggatct
     2461 acaagttctt gaaaaagaaa ccaagccacc acaaccttat accgaaggta cgttgatcac
     2521 tgcaatgaaa acagctggga aaaccgtgga tagtgaagaa gcgcaatcca tattgaaaga
     2581 agtggaagga atcggtacag aagcgacacg ggcgaatatt attgaaacct tgaaacaaaa
     2641 ggaatatatc aaggtagaga aaaataagct cgtggtgacg aataaaggga ttttgctttg
     2701 ccaagccgtg gaaaaagagc cgctgcttac gagcgccgaa atgaccgcca aatgggaaag
     2761 ctacttgtta aagatcggtg aacgaaaagg aacacaaacc acttttctag ccaatatcca
     2821 aaaatttgtc tctcacttat tggaagtcgt accagggcaa atccaatcca ctgattttgg
     2881 atcgacgtta caagaagtga aagcagccag tgaaaagcaa gaagccgcgc gtcatttagg
     2941 aatttgtcca aaatgccagg aacaagaagt cttactctat caaaaggtgg cagcttgtac
     3001 gtctgaagcc tgtgatttta aaaggtcaga agggttcatt tgaagcaacg attgttttaa
     3061 aagaagactt cactacaggc tttgaatttt ctgaaaagaa aaaaacgaac ttcaagaaaa
     3121 gaacacgccg cactgcaaaa taatggaggg ttaatcttat gcgtaatacc acacacaaac
     3181 aacaagaaat cgaaaatcaa aaagccaaaa ttcgttcaga acaggaaaaa atgcgtgcca
     3241 gcaaacgaag actgcagcaa ttgaagaacc agcagaggat tcaaaaagaa atcgaattag
     3301 atcgataaaa tcttcctaac tattgtatac gtataaactt atgttatact gtttttataa
     3361 gtttatacgt atacaaagga gggagaaaat gacaaaagca atggtataca agctgctaga
     3421 agaatttaat ggaactttgg ctttgaagga tgccaaaaaa gcgggcattt ctcctgtaac
     3481 gatcaaacgt atggtagatc gaggggagct ggatcgagag tatccaggtt ttttcacctt
     3541 acctggtcaa tttcctgatg aattgtttat ggcgcaaaaa aaatatgaac gcggcattat
     3601 ttcacatatt accgcgttag atttgtatga tttaacagat atgatcccta gacagatcga
     3661 tctgactgta ccttacggtt atcacgtttc tgaaaaagga ttgaaggaat tcgcagttga
     3721 gctacactat acaaaagcgg aatggtatga attaggtaag attgaaataa aaagtcgcta
     3781 tgggaatcct attatagctt atgatccaga aaaaaccttg tgtgacattt ggaatccctg
     3841 gtataacgtt gaggatgaaa tcaaagtaaa agcaatcaaa aactacatgg agtcggacag
     3901 aaaaaatctt agaaaattga atgagtatcg acgaattttg cctacagata aaacgatgcg
     3961 ctcttatatt atggctttaa attaaaggag aatacacatg acacctacgc aattaaaagc
     4021 aaaagtgaag aatatttcta gagaaaaaga agttgatccg cagcttgttt taagacattt
     4081 tatgatggaa aaattcttgg aaaaaatctc agagtctcct tatcgtgaaa attttgtgtt
     4141 aaaaggcggc ttcttaattg gctcaaagta tggcattgaa aatcgaacaa ctaaagacat
     4201 tgatacgact ttacgggaaa tgaaagtaac caaagaaaca ttgacaacgg ttctaaacga
     4261 tattttttca acgccgacga aagaaggcat tcaatttgag attcaaggaa tgaaagaaac
     4321 gagggaagct gattattatc cagggtttag cttacgtgta ctggcacatt tggaaaacat
     4381 gcggccagat tttaaggtag atgttactac aggtgactcg atctatcctg cgacgatcac
     4441 tcacagtcat aaattgatgt ttgaggatcg aacgattcct ttggaatcct atcctacaga
     4501 acaaattatt gctgaaaaat taagtgcaac ctttgatttt ttaacagata attcgagagg
     4561 aaaagatttt tatgatctct atactattcc taaaatggag aagatcgatg agaagacctt
     4621 gaaagatagt gtgagaaata catttacacg tcgaggaaaa actgatccgt taaagatata
     4681 ctatgaaaag gatatggatg tactgaaaaa tagtcacgag ctaaaagata cctggaagaa
     4741 atatcaaaaa agcaatcctt atgctgcgtc gatcacgtac gatgagacaa tggattcgat
     4801 tagtaatttg atggaaaaag tgattcacgt cgaagaaaaa gagcgaaaac tagcttatca
     4861 aagacaccaa cagatgaata gagaaatgga gagataaaga tgattaataa tgtgacatta
     4921 gtaggacgct taaccaaaga tcctgattta cgttataccg caagtggtac tgcagtagcg
     4981 acttttaccc tagccgtcaa ccgaaatttc acgaatcaaa acggcaattg agaagccgat
     5041 tttattaact gcgtgatttg gcgcaaacca gcagaaacaa tggcgacatt agctaaaaaa
     5101 ggtagcttga ttggtgtagt tgggcggatt caaacgcgga cttacgacaa ccagcaagga
     5161 caacgtgtat atgtgactga agttgtagca gataactttc aactattaga aagtaaagca
     5221 gcgacagaga gccgtgcgca cgctgatcaa tcgtccacca gtccaagcac cgccaccttt
     5281 gaacaacgga atacagcgac gcctaacaac aatggattga acgcttcaca gaatccattt
     5341 ggtggacaat caatcgatat tagcgatgat gatctaccgt tttagtttga gaaagaagga
     5401 gtaagcatgg caaaaaaatt agtctgacga tggatctatt gatacccgta attgcattta
     5461 tagacgggat ttctttgggc gtcactgaaa atcctttggt aaatagaatt gcgaaaggat
     5521 cactatttct tgtattcgtg tatcttgtct gttcttatct tttaaaaaga gcaggaaagg
     5581 ataattcctt ttggcgattg actaaagtca cagtagcagc gacagctgtt atacttacgg
     5641 ctattctacc gctttatatg tagagttaac gggaaatcgt tgaacttctt gcgaaatatt
     5701 gctataatga acatgaaaaa gaggagttgc gctaacaacc cctcattaac agcaccgatt
     5761 aaagaacggt ggcagaatat tagttatttg gaaataaccg ctgacctgct aaagtagacg
     5821 gttatttctt tttgtcattt ttaagcatat ttacaatcaa actaatcaaa gcaatggtaa
     5881 acataccaaa accaagaatg gtttgaattg tctcatatgc ggacaaaagg cttactcctt
     5941 tcatagattt tagcacttac attcataagc accaccacct ttccagaagt cgccaccgtc
     6001 ttcaaacttt actgtcttga ctattatagc atatctaaaa tcgaataaaa attctcaatt
     6061 ttagctgttt ttgctataat tttagcgagg tgatcgtgat ggaacaatat attggtttac
     6121 tcgataaatt agggtgatca agacacagcc attgcttgtg cgatttacat tacgcacgag
     6181 tcatgaacca atcaattgtg tagtggccaa tatagaaata gcggataaat tgctgatttt
     6241 ggaagaaggc aagtatacta tttcagtgac aggtcacttc aataaacgaa agcaactagt
     6301 cattgaatca atgcaaatta gaaaccctga tcattttacg cgatcgatga gcatataaaa
     6361 gaagagctgc agtcctaaat gaggattgta gctctttttg ttttacatag aaacagtaaa
     6421 aatgaaaagg cctgtttagg aaggcacaag cgccttctca attgaaagac ctttctctca
     6481 ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac gctacgcgtt cactaagata
     6541 aagggtcttg catagcaaga caaaatagaa aaaatttcct ctcaccaaaa ggttcgctcc
     6601 tgttcgttat ctctttattg atttgtcaga ccggcaacaa gttgcctttt atcaccaaaa
     6661 agcgacaagg acaagggtaa atggcacgct gcgcgccctt gtcttgtcgc ttttcgttga
     6721 ttcattctgg ccaacaaatc aataaagaaa gaactaacga aaggaagtaa aacctttcct
     6781 aagaggaggc agaatcaatc atgcgaaaga aatttgatta ttggggtgtt cccttcagcg
     6841 aattattccc aaactatcat gcaccacata ctgtcgaatg cgattgtgga gaaagagcga
     6901 aatgtatcaa aagctattgt ttgtatcaat gtccaacatg cgggaaaaaa tataccttgt
     6961 cttatggcga ttatatttta attgatgaga aaggaaagaa acgctgatga aaacacaaca
     7021 caaattattg actgtaaaag aagtcgttca aattttggtg gacaaagaag tcatttctga
     7081 aaaagtagca gaagttttac ttattccgcg gtactggacg atagaaaccc ctttagtaga
     7141 ggatgcaccg actatgctat ttgaagacct agtgaagatt gcaggatatg aactgcatta
     7201 tcaagaaaac ttggaaactt atgaaaacga atattggtta accaaattag agaactaaaa
     7261 aggagaaaaa aatatggctc aaaattacta tgacgaattt gtcaaattac ctttggataa
     7321 aatggcacaa aaaatggagg atatgacgtt cctatacaac gaaacacgtg ttccaaaaaa
     7381 acattataaa gaaaaattgt cggtagctgt ggaagaaatg attgaataag gtgtcgaaat
     7441 gaatcttatt gcgacgtact atcgaacact ggaagaactg aaaaagcaaa atgcaaaatg
     7501 gtttttccaa gccttactat gcttggaagt aggtgtcaaa ccaagcacta ttaaaccttc
     7561 tgaataccaa gctttagaac taacgtatgc gaaatttatc gaaacgaaaa aagccaagac
     7621 tgtttcaagt gaatggttag attattttga aaatatcaat aagtatggcg catattacac
     7681 catgaaaaag gaagacaacg aaaatgaata aggtgattta tcaactaaca acggattcca
     7741 tatcaacgat ttgaaagaaa aaaaggtcaa taatcattta taccgaaaag tgagaatgtg
     7801 tgaaacaagg acaaaaaacg cctagtaact aaggaaatgg ttcttatttc gcaccaatcg
     7861 ctccaaccgc ccccgccgct taggtggtgc gaaataaacg ggcaaagccc gataacttaa
     7921 gagaaagaag gtgatccatt tgaacaagat tattccctta ttgttagtac tgtttgttgg
     7981 cgaaatactt atgcgagata gtgcagctgt acagttaaac ctcgctgtat ttttggtaat
     8041 actggtgttt tgtttgatct tttggcggtt tgtattagtc ctcgtgagtc tactattcaa
     8101 gatgattgca gccatatttt tcatagggtt atttcgatga aaggagtgag taccaatgcc
     8161 aattgcaata ggaaataaac ggttacccgt gacattagat gaaaagagac aaaaagaatt
     8221 gcagcaacta aagcagaagt atggcaaaag tgaatctaag attatgtgta ttgcgttaga
     8281 tctattgatc gcgcaagaaa aagcaggatt tgatgtacca gcgctaaaaa agtgacgtca
     8341 ctttttaatt tgatctatat aagtgatatc acttttacca aggagggaat taaatgttaa
     8401 aaactaaaaa gaattcgtac aaattaaagc ttagtatact atctattatt tgtcttttag
     8461 gcatgataat tagtggtcat ttgtatagag tggcatatac gcagtttatt gagtacaagg
     8521 ttcactcttt aggattagct ggggatcgat acgttgaata tgtccattat tcattaaaat
     8581 gttcttcata tattcttctt tggcttattt tttcattgtt tgaattgcta aatttaatag
     8641 caatatcttc atatcactta gataagctaa ataccaaatc tagccgttat aacgaagtgt
     8701 ttaaggaaaa tgtgaagtta gaaagtgact taaacaagct aaaaaaataa aaagaagaaa
     8761 acataagtta actaaaatta ttttattaag aatgatggaa tataaggata aggttaccaa
     8821 cataacgatc attacttctc tgatatgcta tggacttcta tcaatcagta ttttgtctat
     8881 agattatgga gtaactatac tgcaagcctt agttttggct atgcctatgt atctatcttt
     8941 gtttatcatg catgaaatat ataagctatt tgttttatca aaaaatg
//
LOCUS       DAFEPQ010000087         8656 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.089, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000087
VERSION     DAFEPQ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.089"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(<1..432)
                     /locus_tag="LP543_002402"
     CDS             complement(<1..432)
                     /locus_tag="LP543_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL2322539.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADE"
     gene            complement(444..848)
                     /locus_tag="LP543_002403"
     CDS             complement(444..848)
                     /locus_tag="LP543_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322540.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            complement(841..1242)
                     /locus_tag="LP543_002404"
     CDS             complement(841..1242)
                     /locus_tag="LP543_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322541.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            complement(1232..1585)
                     /locus_tag="LP543_002405"
     CDS             complement(1232..1585)
                     /locus_tag="LP543_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBL2322542.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            complement(1575..1886)
                     /locus_tag="LP543_002406"
     CDS             complement(1575..1886)
                     /locus_tag="LP543_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322543.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            complement(1883..2758)
                     /locus_tag="LP543_002407"
     CDS             complement(1883..2758)
                     /locus_tag="LP543_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322544.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            complement(2768..3928)
                     /locus_tag="LP543_002408"
     CDS             complement(2768..3928)
                     /locus_tag="LP543_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBL2322545.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            complement(3928..4614)
                     /locus_tag="LP543_002409"
     CDS             complement(3928..4614)
                     /locus_tag="LP543_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBL2322546.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            complement(4577..5755)
                     /locus_tag="LP543_002410"
     CDS             complement(4577..5755)
                     /locus_tag="LP543_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL2322547.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            complement(5775..7469)
                     /locus_tag="LP543_002411"
     CDS             complement(5775..7469)
                     /locus_tag="LP543_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBL2322548.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            complement(7447..7761)
                     /locus_tag="LP543_002412"
     CDS             complement(7447..7761)
                     /locus_tag="LP543_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBL2322549.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            complement(7864..8145)
                     /locus_tag="LP543_002413"
     CDS             complement(7864..8145)
                     /locus_tag="LP543_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322550.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            complement(8150..8494)
                     /locus_tag="LP543_002414"
     CDS             complement(8150..8494)
                     /locus_tag="LP543_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBL2322551.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
BASE COUNT         2484 a         1802 c         1244 g         3126 t
ORIGIN      
        1 ttcatccgca ccattttcaa tagagaatgt taatcctgta ttcgaagtgc atgctaacat
       61 accaactaac tttctatttt cttcgaagtc atccacgatc tctgcagcaa gtgagaaatc
      121 ttcgcctacg gaatcaggac cgtaagagta aatgcctggt ttaatacgtc catcttgttt
      181 caacccattg aaacgtcgat aaacttccat cggtacatgt gcagtaattg ttaccgtcat
      241 attgattggt ttagattttg attttacttc tgtcgctcca cattttttaa ccaccgtttg
      301 catttctgtt tcgccatcta attgtccgtt acaattcgtt gcgattgcat ttcctgcgtt
      361 cttaaaatta aaagcaattc gtttgatact tacgttatcg aacgttgtta ctacagtttt
      421 tgttttagcc attgttgttc cccctattta tttaatttat cgaattgacg aatcagaagt
      481 tctgtaattg gatcaagtgc aagacctaat cctcttctca taaattcgtc cggctgattt
      541 cttttagaag tacctatccc caaatcagga tattttaaat actcaaattt tcttgtaggt
      601 ctaatgatga aacccaaatt aatgtattga gtcttaagtg gacgactatt ttttgcgtgt
      661 tggtgccctc ttcttaaatc tgcttcagaa acaggaattt tttctgtaat cctatccact
      721 gcaatagccg aaccttttga tttcaatgct tcgttaatca gtcgttcgct ctcgcttgaa
      781 tagcgttcca tccgcacaag aagttcatca tgtccattta tttttagctc ccaactattt
      841 ttagccatga caatcactct tcaataatcg tctaaacgta aataccaatt gatcgatata
      901 gcgatcttgg ttctctagtt ttaaatgatt gggatccatt ctctgaaaac gaatcgaacg
      961 attttgaatc aatgaaataa tatctagtga gtctcctgtt aaatcttctc tattttctga
     1021 atagaaagtt agatatagat tttgacccac gctatatttt ggctcagtga tcatttctat
     1081 ttctcctgtt tcgagaatga agtaattaaa atcatcaggt agctcatcct cgcctacgga
     1141 gtcttgaaag agtttgaggc caaaatgctc ttctaaggaa gttttgatag cagaaatttg
     1201 cttatttaaa cgttcttttt ctttagaatt atcaatcacc atattcaccc acactttcaa
     1261 gataaaaata gatataaaaa ttatcgtaat cggcatagat aacgttgtaa cgcatactat
     1321 cgattacgat aaaatattga tctttattaa atttcttggc gattggatga aatggagtct
     1381 ttactttctt agttaatttc gatcccatcg catccatagc tgttatatca ctatctctca
     1441 tggaaagatt tctaaatttt aaagaagtga tttctgtatc ttctacacca atcttttttc
     1501 ctagttcatt tcttttggta gtttgcgtca aaatctttaa ccaaccatcg ttgaatgttt
     1561 cttcgagtct acgattattc gccattcaca tcacctgcaa tatattcttg tagcgcataa
     1621 tgttgaatga aacctaataa ctcactagcg aaattttgtt caaactcatc taaagcacga
     1681 ttccagtcgt atctacatct ttcgattagc aatccgtatt ctaagctttc aggagaaaaa
     1741 gaaagtgttg tactcacttt actttgaaga taaacagcat ttttagctat catcttttta
     1801 attgactcat cttcttcgtt ccaggtaacg taaatattat ccttcacagc tattagcaat
     1861 tcttcagtca cttgttcagg cgtcatctaa ccaccgcctt aattgcttta acatatgcgt
     1921 aagagcattt tttcttgttt acaaatgata aatcttcatc aaaaggcgta gaagtcacgt
     1981 atctcccttt gaaaaataaa tcttcatcgt ttgttgttac tccagcattg tgtaagattt
     2041 ttacttcttt aactttttct attggatcag tagcaaaaca aaagtctaat tcctcgtgaa
     2101 ctttaggacc aatattgaaa tacatcatgt tccaaagctg tgcccacatc tcggctgtcc
     2161 agatttgtat atttgttttt tgccctctaa ggtagcgata tagccgatta gaatccagat
     2221 aaaccttttt ccaataattc gctttaggac ggttaataac ccactgtgcg cctcctgaat
     2281 tagtgtttat agtttccaaa gattctactg taacatttac aatgtttgcc atatctttta
     2341 gaatattttc tccgttttca cagcttctaa tataatcaag acttagataa ctacagcagt
     2401 cgctacaata ccaaacatca tctttagaag gcaatttgcg caaattaatt cttttattga
     2461 aaatgacatc cgaatcgata tagaaatatc ggtcgtcctc acgcgaatga tcttcttcta
     2521 aatatttcca ccataaatat ggtttaatcg aaggaatata ctctttgtcg tcccgcagat
     2581 catcgtacac atgaacttca acaccatatt ccttctcaaa aaaaatagga atctgatcat
     2641 cgtgtctgct gaaaagcaat acgatatctt tgattcctag tttcttcaga ttagttaaac
     2701 aaacttcaag ctcccattta aaccgattga ttgccggctg acaaagaata tacttcattc
     2761 tgatcaccta cgcttgtgtt gtagttgttg tggttgttgg ttttgtagtt gtagtagtag
     2821 ttcccaaagc gctaatatct aatacaatga aactatcgtt acgtttaggt tgaccgtttg
     2881 catattgttt agctagataa atgcgttcgt cttcaacaaa atggtattca tctgaagctt
     2941 caatttttag tgtagatcct acacccatga agtaatctga ggctacccca ataactgctt
     3001 ttccttctgg cacagccgtt gactgcaaat ctgaaactgg tactggcaat acttgtacgt
     3061 attctccatt agcagttagt acagtcttag ctgggaatac tttagaccag taatcagttg
     3121 gattcacaat taggaccaca tcagaaggat tcacattacg ataaatcgga tcattcacac
     3181 cttcgatatt gaattttgat agtcgcgcca tcaaaccgcc catagttaca gcatctaaag
     3241 ctgtaatagg ttctgctttt ttttcagcat attctccgct agtttgtttg ctcatgtcac
     3301 gcatcattcc gactggcata tctttaccag taccatcaac aattgcttgt tctaatgcaa
     3361 ttctcaatga ttctactaaa acagtacgga cataacgatc taaccatact ggacctaaat
     3421 caagcattgc cttacataca ggaatataac ctgatagctt gaactgcttc atgttaatta
     3481 catcaaagcc attatctaaa acttttttaa cagcttcgca aagtttaccc caccatgctg
     3541 gattgactcc acgtgacaca atccattctg ttacaccagt tgtgttaaca aaagtaattt
     3601 tttgcaatag tggatgagat tgttctaaat cttcaaatac acgttcaaat acagtagctg
     3661 gcactaattc ttcgacccct gcaaaacctt cgtttttcac tacttcgtta tagaattttg
     3721 tttcttgtgt agttaataca cgctgaccac ggttcattaa tactaattga tcttgatttt
     3781 ttgctgttgc ttcttctaaa attttatcct gaatttcctt agataagctt accatagctg
     3841 cgctaaaaga ttcttcgtta ccatctttaa aagctttcat caattggtcg cttgcagctg
     3901 ttacaccttt taaattttta actgtcatta ttttacatct ccttgtccaa atgttttatt
     3961 taatgctgct gtaaatgcag caattttttc tgctcttttt tctttaacgt cattcaaaat
     4021 ttcttcaacg ctttgttctt ttttagcttc agtacctgag ctattttctg catcgataat
     4081 ttcatcgacc aatccataac tcaaagctgt ttctgcatcc ataaacgatt ctttttcaag
     4141 aagttcttgc aatgcttcat ctgtgccatt gaatcgtgtt ttatatgaag cctttaccga
     4201 tttatcaatt gattccagtt ggtcagcaat cgtacggaag tcatcgacat ttccttctcc
     4261 gtatgtggaa gcgcggtgaa tcatcaattg tgcattgttg tagattttta tagtatcgcc
     4321 agccattgcg ataattgaag cagcactagc ggctaagccg ttaatcacaa cgttaacttt
     4381 tgctttattt gacttaagta agttcccaat agcaatccct tgaaatacgt ctccaccgtt
     4441 tgaattaatt actacttcaa tttcttcttg atcacctaga ctatccaaaa tatttttgat
     4501 tcccttgtca gtattccctt caaagaacca actagaacca ataaatccct gaataaaaat
     4561 ttgcggtact gcgccttcat tctttactgc tagaaatgtt ttcattgtcg tcattcgcct
     4621 cacctccttt cgatacttgt tgattgtttt tagttataaa tatttcatct gccatcgcct
     4681 tatcagagcg atcatttcca acgcgttctc ttccttcgtt gattgtaaat actccatttc
     4741 taatgcctac atcaatagcg tcaaccaaat ctttgaagct agtaatcttg atcatagttg
     4801 tatccacacg tacaaaattc cctgacaagt attcttctac ttcatagaga ctagcgttaa
     4861 acgcatcctg aataagttca gcaatcggta tgatttcgaa cattaaaaaa gcgtccactt
     4921 gatccgataa cccactcatg tctcccttta gtaggttttt cggaacgtga aacgctgctg
     4981 ctgtcatctc aaagatgtcg tctattaagt tttttatatc tcttgaattg ctttggaagt
     5041 ttccgctgaa atcttctaat gtgtactcat tttgtaattg aaataccgca cctgcattat
     5101 cagcttccat aaaagcctta aattgtgatg tcatcatttt attgatttga tcttgtgttg
     5161 tattgtcttg cggtcggaat aaattccctt tcagtacgta tctacgagcg ttagagcgct
     5221 tgtaaacatt catggcacta gaaatgagtt tcccatacgc ttgataatac gcatcgacta
     5281 gttgcctaat ttgttgatct gcgtatttta tatagataac atcactttct agaaattctc
     5341 tatcaaggac tatgttgtta atttgcactt gagaaaacac atcatctttc aatgcatatt
     5401 ctgtgacatc ccaactatcc gcaataaata tttcgctaga attattagac ggagaaacga
     5461 tcaatacttc attgtagaat attaatctcc tgatcagttt ttttctaaat tctgttgcat
     5521 tatttttctt attaggagct acattcagcc tatagtaaag atcattcttt ttattttttc
     5581 catcttcata tgacttgaat tccgctttac tcatcgcatt tgcaatcaaa tcaatacaag
     5641 tttcaatcgc aaattttcga tacacaaaat caacttgcaa tttacaaaag tattcttcta
     5701 aaggaaccgt tgcttttttt gtgaagtatc ctaccgcctt ttgaaaaatc cccactttct
     5761 cacctccttt caagttagaa tactagagga gtaaatccag ttcctgtatt ttctactgag
     5821 ctatttgtga ctgttacagg agcagaatca taaatatcat ctaaaaaatt caaaccatga
     5881 aggaatgaaa aaaagccatc cgtttttcta gtttcaggtt ctattttttc atagcgtata
     5941 tttccattag aaatatgctc ttcatataca ttcatgcaat accaacgcat aatcgcatcg
     6001 tcaccaaaaa ataaacgttg attaataaaa aggtcatcaa ccagatcttt taacatacca
     6061 tgtgtaacag atccgcttcg aacaatttcc acagtaaaac ctgcttcttc taaagcgggc
     6121 ttcaatattt ttgcacggta catatccata gcgatttttt taatataata tttattactc
     6181 atttcaagaa accaacctac aatataatca gcttctatat tttttccatg aacgatctgt
     6241 gattttcctt gatctataga aatatctata acctctcgtt tgatgttttg taatcgaagg
     6301 gctgattcgt ggataaaagt atgttgtgta aaataaacat ctttatcgta ttttcctagc
     6361 aacccaacgc tggcaaaatc tcgtctatca gcaaaatcga ctgttcctat cacttcatcc
     6421 attttttcag gaaattcttt ttctttcgta tgcagaacat catcatatga agcaacagca
     6481 aatcgtgtat cttccatagg tctgttcatt cgtttggtca tgaacgtaag tcttaaacca
     6541 gcattacgtt gcatttgaga gtattcttga aacattttcc gttttaaatc tgcattgtaa
     6601 ttaatagttg gacaagcttt ttcccacatg tcgggatcat caacttcatt atcgttatcc
     6661 aagcgacaaa taaatggaaa caaactagaa aattctgctc catccttgtc aattccaagt
     6721 tctccagaaa gaatcatttt tgattcttct ataatgtcat caagcggacc accacgaaca
     6781 tgaccattag ttgtatcata aaattctcta taatctcgaa ttttaccacc accagaagta
     6841 gccacattta tcattgaata atcttcattt tcgtgaattt catcaaagcg gtttgcacct
     6901 ggtcgcttcc catcttttgt tctagcattt gccgtgttat aacgaagttt gctgtttgta
     6961 gcgatatttt gaataacttc cttcgtagct ttaaatactt ttttatctaa atcaggatga
     7021 tctttaatta ctttaaatac atcatcaaaa ctagtctttg cttggctttc attattggca
     7081 tagatatcaa tatcataatt tttaataccg tgtttagcgg ttagtagaaa gaagttgttc
     7141 caagaagcaa aaccagtttt accattacca cgtcccatta atgaaagata tctattgaac
     7201 actagtgttt tatctttttt ccatcgaaca ccataaataa aacattgtag aaatttttcc
     7261 cacggaatta attcgaatgg aaagtattgt gctggtatat tgattgaatc ctctaccatc
     7321 tgcttatcga agtaaatatc ttctctagta aagactcttt cttctagata attttttagc
     7381 aataattgct ctttgcatac cttgatagtg ccttcttcta tagctttgaa ccaattttca
     7441 atatgcttat aactcaggaa ttgattcatt tgcttcacct accaattcag gagtaatggc
     7501 aagtttatcc aacatcaatc ccatttgttt gttgacagaa acaagcaacg ctactgattc
     7561 attcttttta ccattctcca gtctaatgcc gttctcggat atatcttctt ccagtgatat
     7621 cgccgtttcc cataaactga tataacgatc aacattatct aagaatggct caatatttgt
     7681 tttctgactt tccaattggc ttattaaaga gcggcgtaat ttttctctgt agcgattttg
     7741 agacaattcg tttttaaaca ttttagccct cctttcatga taaagttcga aaaaatctct
     7801 tttcctgaca gccccctccg tttcatcacc cccaaaaaat ttgcgattta ttttaagggg
     7861 gggttatctc accatcggaa tgaaagcttc agcgaagtca atgtaataat taatctcttc
     7921 aatactatat ccaaaaacat tttttattct ttcgacgtta ttatctttat tcaacgcttc
     7981 tcttacttga ttcactttgt atttactaca acagttatct gataacagat ccctaatacc
     8041 tacatagcga acgtatatca aacgtttaat taatccctga gtataagatg aatactcttc
     8101 aatcttttct gtatcatact ctctgccatt atcattgatg atcatgcact taccaccttt
     8161 cacttgcatc gaagttagca aagctttcta tcttcttctc ttgtttatct aatgctgtaa
     8221 gatatctgcc atgaacttca ttatgatgtt caacacataa acaaataaga ttatctaaat
     8281 ctaaagctaa gtcaggtcta tccttgactt cctttatatg atgaacgttc tctactctat
     8341 gatacttacc tagtcttcta cactcttggc attcatagtg atctcgtttc atcgctttct
     8401 ctctaagcct gcgccattta ggagactgat agaacttaac caaacgatct tctcttgtca
     8461 actgtaatag ccatctatag aattcctcgg tcatgttccg tctcctttcg caatcttatt
     8521 taatgcttag ctattctttt gccatacaat ggaataactt cattgttttc ctttcgttta
     8581 tatgtatcgc tctttattgg tcttctatac tttcgtacta tctcaccgtt accgttttgc
     8641 acagtgatta cttcat
//
LOCUS       DAFEPQ010000088         8400 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.090, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000088
VERSION     DAFEPQ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.090"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(401..717)
                     /locus_tag="LP543_002415"
                     /pseudo
     CDS             complement(401..717)
                     /locus_tag="LP543_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(718..972)
                     /locus_tag="LP543_002416"
     CDS             complement(718..972)
                     /locus_tag="LP543_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL2322552.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            1331..1735
                     /gene="tnpA"
                     /locus_tag="LP543_002417"
     CDS             1331..1735
                     /gene="tnpA"
                     /locus_tag="LP543_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HBL2322553.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            1752..2900
                     /locus_tag="LP543_002418"
     CDS             1752..2900
                     /locus_tag="LP543_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL2322554.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTIALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(3123..3341)
                     /locus_tag="LP543_002419"
                     /pseudo
     CDS             complement(3123..3341)
                     /locus_tag="LP543_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3607..3801)
                     /locus_tag="LP543_002420"
     CDS             complement(3607..3801)
                     /locus_tag="LP543_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322555.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(3901..3987)
                     /locus_tag="LP543_002421"
                     /pseudo
     CDS             complement(3901..3987)
                     /locus_tag="LP543_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            5153..6193
                     /locus_tag="LP543_002422"
     CDS             5153..6193
                     /locus_tag="LP543_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL2322556.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            7017..7349
                     /locus_tag="LP543_002423"
     CDS             7017..7349
                     /locus_tag="LP543_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBL2322557.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            7968..8309
                     /locus_tag="LP543_002424"
     CDS             7968..8309
                     /locus_tag="LP543_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBL2322558.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         2977 a         1267 c         1417 g         2739 t
ORIGIN      
        1 ccatagatat attggacttc ctcttttaaa acacaaaaat aaatactacc ttatttaggt
       61 agtatttatt aaaagttacg tcaattttga ttaaaaatat actacttgaa cctctcatga
      121 gtaaagtcac gaaatttttg ggaatagagc gtacttgata tcttattttt tcactaatag
      181 cagtgtctct tattcaccaa gcgatccccg tagttcctac ggttcttaat ttttctctag
      241 actaatgatt gtatctttag accttcggcc ataacatgga gcaaaataaa aaggtactaa
      301 gtcttatata tcaatcttcg agtaaccgac ggttaacaac catatcgagc ggcattaatc
      361 acccacctgt agaggatgag cgactttttc ctaattattg ttaaaagcct agtttcattt
      421 gtttagattg gaaaatacgt cctacaagta tctctttttt tcatggtcga tccgataaaa
      481 taaggcatag cttttgccga tcacgatccg atatgttggg atatccattc catagatttc
      541 ggaaacttcc tcatgcatag ttggaaaatt tttggttagt tctaaagcct tctggatcgc
      601 acctacaaat ctttttatgc tggctggaga aagagcaagc tcattttccc attcttctat
      661 tatcgtttca aggctggtct gaaaactttt agaataaact agcgtgtact ctttcatcta
      721 ttcactccag ccgtattttc cgaaagcttt taccatttca atttctgaaa ctgttaaacc
      781 tttctcaaag tcttggaaac tttcttcaat caatttgcga tcgtgttctt ttgctatttg
      841 agtagtatct aatatgaata agttgtcttc ttgtttggct tggataatgt ctccgctttt
      901 taatccaaga gagcttggaa ggatcactcc cacgctattg ccgatctttc taacctttag
      961 ttcttttccc atgttatcac actcctttta gtttttgatg aataaattat aacatgttat
     1021 aacaacaatt gccaacggtg tttcttaagg gattccactc catattatta ttggacaaaa
     1081 taagccactt agaacgctga aaacatatac aaacgatatc aaaatacgag tgaactactc
     1141 accatacact tccgttaaca tccatttaac agaattaggc agaagtcgcc catcctctat
     1201 aggtgggtga tgaatgccgt tcggtatgag ggttaccgtt ggtaactcga agaccgacaa
     1261 ctaaggcgta ttgccttttt attttgtccc atgttacaat cagtctatac aagaaatgag
     1321 ttgatataac atggaattag atacaaataa tcattcagta tttcttcttt attaccacct
     1381 tgttttagta acgaaatatc gtagacaagt gattgatgat gaaatatctg attatgccaa
     1441 aactactttt gaaagaattt cagaatcgta tcacattact ttagtggagt ggaatcatga
     1501 taaagaccac gttcatatta tgttcaaagc tcaacctaaa acagaattga caaaattcat
     1561 caatgcctat aaaagtgcca gctcaagatt gataaaacgt gactttccaa gagtcaaaca
     1621 atttctttgg aaagaaatgt tctggtctaa aagcttttgt cttctgacaa ctggtggcgc
     1681 accaattgat gtgattaaga agtatatcca aaatcaagga aacaatcata aatagaaagt
     1741 aggtgaactt tatggaacaa ctaaaagcat acaaattcag aatctatcca acagaggaac
     1801 aagaaatctt ctttgccaaa tcttttggtt gtgtccgtaa ggtctacaat ctaatgcttg
     1861 atgaccgaaa aaaggcgtat gaagaagtta aaaatgattc ttctaaaaaa atgacttttc
     1921 cgacaccagc taaatacaag aaagaatttc cgtttttgaa agaaattgat agccttgctt
     1981 tagccaatgc ccaacttaat ttagataaag catacaagaa tttttttcgg gataaatccg
     2041 ttggatttcc acgtttcaaa agtaagaaaa atcctgttca gagttataca acgaataatc
     2101 aaaatggtac aattgctttg attgatagta aattcatcaa agttcctaaa ttgaagtcat
     2161 tagtaagaat taagcttcat agacaaccaa aagggattat caaatctgct acaatatcac
     2221 gtcattctag cggtaaatac tatatttctt tgttgtgtaa agaagaaatt atcgaattac
     2281 ctaaaactaa ttccacaatt ggtattgact taggtattat ggactttgcg attctttctg
     2341 atggacaaaa aattgataat aataaattca cgtccaaaat ggaaaagaaa ctaaagcgtg
     2401 aacaacgcaa attgtctagg cgtgctttat tggctaaaaa taaaggtacc aatctatttg
     2461 aagctaaaaa ttatcaaaaa caaaaacgca aagtagctag attgcatgaa aaagtaatga
     2521 atcaacgtac tgattttctg aataagttga gtacagaaat gatcaaaaat cacgatatta
     2581 tctgtattga agacttaaac acaaaaggta tgttgcgaaa tcataaatta gctaaaagta
     2641 tttctgatgt ttcttggtca aagtttgtga ctaaattaca atacaaggct gactggtatg
     2701 gacgaaaaat catcaaagta gataaatggt ttccgtctag tcagatttgt tcagagtgcg
     2761 gacataaaga tggcaagaaa tctcttgaaa ttcgagaatg gacttgtcct gtttgtcata
     2821 ctcatcatga tcgtgatatc aatgctagta tcaatattct gaccgaaggt ctaagactac
     2881 attctatggg attggcttag atccaatcat caagaaccgt aggaactacg gggatagctt
     2941 ggtaaataag agacaccgct gttagtaaag aaatacgcta tcaagtatgc tctattccca
     3001 agaagctccc acttcaagcg ttaagaaccg tcaggtttag ctaagtggtg agtagttcac
     3061 gtaggtattt ttattatata tagatttaac atatgtcggt cggttgattg atatagctaa
     3121 agttataact caaccgaagc cgattcatct acacgaataa agttgcgagt gttctcggct
     3181 aattcataaa aattgttatg tgttccatat agcaatactg gaataacttt attttcttga
     3241 atctcataag caactctata cacggtgacc gcatacttaa atccattaga ccaattacct
     3301 gataaacctc cagttttttg ctcttactta aatggatcaa tggcaatttc atcacaaacc
     3361 tatagaggat gagtgacttc tgcctaatta cgttaaaaaa tattgaaagt aactctatgt
     3421 agcaaaaaat atctagaatt tcatttcctc aaaactgatc gatattgtat ctaaaaatat
     3481 atactaccta aaataggtag tatatattaa aatatgtgct tttgttagaa aaatcaatac
     3541 ataaattatc atatctgtat atgtcattta ggtatttcag ttatgttata atagttttgc
     3601 tgtctgctac cccggcctaa tctggcaagg ggaggaggag atataatgaa ataccttttt
     3661 gattttgttt ttgcaatcaa gccacttgca gatgtagtgt gcagttacct ctgcaaatgg
     3721 cttgattgca aaattgccaa aaaagacagt aagcctaccc gttaattcta taatatagaa
     3781 acgtaaaaaa aaccccatca gcctgcacgc tgatggggtt tggtgatata atgaaatacc
     3841 tctttgttac tcataattgt accataatta gtcacgaatg caaccaatag aaaaaactac
     3901 ttagaacggt agatcatcgc taataacaat gcattgtcca ccaaatggat tttgtgaagc
     3961 attaaataca ttgttattaa ttgtcgctgc ttttaactag gcctctcccc ttctattata
     4021 aaagaagata tatatcattt cttaattacc tactacctta aaccaaaatt taagtactac
     4081 ctaatttaag tattacttaa acaatctaaa aaatagttat cccaattgtt atgcttttat
     4141 aatacgatta tttgtaacaa aatgaatatt atataattaa acactaccat atttaggtag
     4201 tgtttaatta ttagcaaaac aaaaaatatt tattctattc tcttaacttc aactaagtta
     4261 tgctgcacac ctttatataa atcatatgca actcattagc tagacaaact acaagcatgt
     4321 agttttatct gttaattatg tactacctaa ataaagtagt ttataattag caagtatata
     4381 tatttaaaaa tttaagatta atttttttat gacttgtaca aatttatctg atattctctt
     4441 attaagaact acctaatttg ggtagttctt attttttaca ttttaagtag tatttaattg
     4501 caatttggta gtattttttt attatcttat tcttagaacg gtagtactta atataaatat
     4561 aggtagtatc taatttaagt attaatggta gtatctaaat taacaggtag tatgtaacta
     4621 ctttaaagta atttttttca gattaacaca gtagtatctt attaaataag ttaattttaa
     4681 tggtagtaca taattattat atatatctga cattggtagt acttattgta ttttttagta
     4741 gtatataatc tattgttttc tagcctaaag ccttgtctta taagaataat ataatatata
     4801 ctactgaaat aaggtagtat atattactag taatggtagt atgttttata tgcataaggt
     4861 agttcttaat ttattttttg ttttgacact ggtagtactt aattatcttt aattttaaaa
     4921 ctaaatttta ttcatacgtt tttctggtag tatttaatta taaataatat ttatttaaag
     4981 tagttcttaa tgactttaaa ggtagtacat aattcatttt atttctgatt ttaggtagta
     5041 tttaattaaa aaacagtttt aatgcgtttg cattttttat ttatctttgc tattttcgaa
     5101 aagagtaggt aattttacat aaataaaaca acgtgaaagt agaggatcta caatgactga
     5161 ttttaaattt tttaaggcgg atagagttta caacgaatta ttttatcaat ttccaaaagt
     5221 ctttattgtt tctgacgaat acaaaaaaat gaaagattca actaagattg cctatatgct
     5281 tttaaaagca agattagaga tcgcaatcag caaacggcaa atcgatgaag aaggtaatgt
     5341 ttattttact tatacgacaa atgaactatg tagagtatta aactgccaaa aacaaaaagc
     5401 gatagcaatc aaaaaagagt tggaatcctt tggtttatta ttacaaaagc agatgggatt
     5461 taacaaacag ttagggaaaa ataatcctaa tagactatat ctagcagaat taaaagtctc
     5521 agaaaatgat atctacttac tcgaaaaatt tgatagagag aatagggaaa acgttgataa
     5581 atcagagggt atgaaaatca tacccaccct cgacgaaaaa tcagacgctg aatcccttgg
     5641 ggctcaagag ggtatgaaaa tcataccgtg ccaaaacgtt gataaatcag agggtatgaa
     5701 aatcatacca gaacttaata ataatatatt agacactaat agacacaata tagacactga
     5761 aaaagaccgc ctacaagatc aattgttgtt agacaatttt gagacaatta tgacaaacga
     5821 cagcattgct acgtttgtcc ctgaacgagt attaaatttg ataaaaacat tttcttcaag
     5881 ttacagtgaa gctcaaaaaa ccgtccagac tattcataat gcaaagaaaa aagctgaaat
     5941 agaaagtggt atttcgatag tttttgaaga actcgatagt tattatgtca atgcagaaca
     6001 agaattatac acgacactgt taaaagccta tcaaaaatta aaaaccgaaa aagtcgaaaa
     6061 tatccagaac ctgatttttg tctatgtaaa aaattggttt atcgaaaaac caatagctgc
     6121 taaagtatca agtgaaaaac gtttgaatta tgaaagctcc ccaagcacta ttacgaaaga
     6181 ctggttagag tgaaacttta ctgacaaagt gattgttgct tcatggagta acattaaaat
     6241 taaaaatttt ttattattct aattatatta cgaatattac aaaataatag ctattttaat
     6301 agaatttata agttaaaata ttatgataaa aatcacaaac gtagcctttt acgtcataag
     6361 tgttaaaaga aataattaat ttcaaatata gaaaagggaa cgtatgtttg catactcctt
     6421 ttttttgagt tataataata cgcgaagaga gataatttca ttatttaaac aagctaaaaa
     6481 tcttgatatt acttcttttc aggcaatacg aatattattc gcttacattt tttgtataaa
     6541 tttaattgtc tgctatgctt gtgatgaatt gattttcgtt ctgcactttt ggatgataaa
     6601 tttggacaat tgaaaataca aaaaaaagaa aggcggtatg aactcggaag gctggtaacc
     6661 ttcgtcagcg ttcatacacg gcattaagat ttggccctta atgcgttaac ctttcagtgt
     6721 tacggtatta cccgcaatgg atttagtata acagatattc aaaagtgata ccactttttt
     6781 tggctgtgtt aaggttaaaa atacgtgata ttacgatgaa tctatgccta agcacaatgt
     6841 tataaaaatg tccgtgtggc tctttgccgt atgacgaacg ctgaaattag agggaacgca
     6901 tgtttggatg cgttccctct tttttgtttg tcatgaacga aaatcaattc tctatttttg
     6961 aaaaataaca agaaaaactt gttaaaaata tttaaagaaa ttgggtggtt tattttatgt
     7021 catatacagg aattttgagt ctcaaagata tttgtcacta cggtaaacgg tgtaccgcta
     7081 cagagaaaat cacgaagaaa ctatcaactg gccaaagtaa aaccgttgtg cagtgtaaga
     7141 aatatataat ccagaaagac aaagtctcag aggaaatgat ttattacatt gggaaacaaa
     7201 agcaaataat tttaaaagat cctatcccct taaaagagct atatccaaca atcaaacatg
     7261 tttatgacca aaacggtgta ctgattggtc gaagaaaaaa tggagtcttg cgttgtaccg
     7321 caaaagggat ggggcgtttg atcggctgaa ttgatcattc tcataacttg tattttatcg
     7381 tttttcgctt gcttgaaaat attgtcgaga cttctaaagg aacttagagt cactagaaaa
     7441 ataaggagta gacttgatct atcaacaatt cccttcgggg agtgacgggg taggttttaa
     7501 cgattggtaa cgtaaaaaat tggtcaatcg agtaaatgcg cttgtttgga tagtggtgaa
     7561 atgaatttgt gtaaataata agcgcagagt aaacaagagg gaatttagaa gttagacaag
     7621 ttagaatagc gtctagatta atcatgagca gctactaagg gatctagact ttgacgcaaa
     7681 ctcatggaat aaggagtagg tcttctcgta atggggagga cgcacaagaa attgtgtcgt
     7741 gtgctgcagt acatgtagat acttgcgagg acgttgatta ttggatcaat cgagcattta
     7801 ccgctgtcta ctagggtttc tcgtttcgga cgccataaac gagtcgaaat tttttatgta
     7861 tttttaagta aacgaaaaac gataaaatat gaacaagtcc tgaataaagt gaaaaaattg
     7921 atataaaaca tagagaaaat gaaatttacg taatggagga atgacaaatg aaaaattatg
     7981 atccgaatat acgattgggc acacatacta ttaaggtttc tttccaacgt tgggattaca
     8041 aaggatttgt gacgtttaga agaggcggaa attgtaaagg attagatgtt ttagcattag
     8101 acgaggatga tttatacgat caaacattga ctgataatcc aattggattc ggcttgctac
     8161 ctgaagatga tgaaggtaat gaatggttta aaatgacgct tatgaatgat aatggagacg
     8221 aattatctgt tgaagatact tggtcatatt tgagtgatta tattgtcagc tttgaaatca
     8281 ttgaatttgt agcggataaa gaagaataaa aactggagga ataaatttta cagataaagg
     8341 cttagaagga gtcacgatga ttaataaaaa ggtagtatta ccttaggcat tgagtaagga
//
LOCUS       DAFEPQ010000089         8359 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.091, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000089
VERSION     DAFEPQ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.091"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     regulatory      211..290
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            288..1481
                     /locus_tag="LP543_002425"
     CDS             288..1481
                     /locus_tag="LP543_002425"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBL2322559.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1611..3083
                     /locus_tag="LP543_002426"
     CDS             1611..3083
                     /locus_tag="LP543_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2322560.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3166..3954
                     /locus_tag="LP543_002427"
     CDS             3166..3954
                     /locus_tag="LP543_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBL2322561.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4021..4689)
                     /locus_tag="LP543_002428"
     CDS             complement(4021..4689)
                     /locus_tag="LP543_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBL2322562.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4804..6516
                     /locus_tag="LP543_002429"
     CDS             4804..6516
                     /locus_tag="LP543_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2322563.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6519..8291
                     /locus_tag="LP543_002430"
     CDS             6519..8291
                     /locus_tag="LP543_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2322564.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2566 a         1399 c         1832 g         2562 t
ORIGIN      
        1 ttaataccta aaatgaaaat ggggctgaag aatcaattct gattcatcag tcccataaat
       61 tatagagcca ttttaaaaag cttagcagag ttatctttat ggatgattgt gctaagcttt
      121 tttggacagt tctaatgtaa aatattttta tatttttaag caaaaaaatg aaaaaatacg
      181 agcagaaaac tgttttatct gctatacttg gttcgttgca agttcccgaa aggatccgca
      241 ggacggagat gccttgtaac cgaatttaac tagggggaat cattataatg gtagaaagac
      301 acttatttac atcagaatct gtttctgaag gacatccaga taaaattgct gaccaaatca
      361 gtgatgcaat cttggatgca attttaaaac aagatccaac agcacgagta gcgtgtgaaa
      421 cgtctgtaac aactggtctt gttttagtat ttggagaaat atctacgacg gcatatgtag
      481 atattcaaaa agtcgtacgg gaaacaataa aagaaattgg ttatacacgt gcaaaattcg
      541 gatttgatgg agatacagca gctgtattgg ttgcgattga tgaacaatct cctgatattg
      601 ctcaaggagt tgatgaagca cttgagatcc gggatgaaga taaaaaagac gtattagatg
      661 agattggtgc tggtgaccaa ggtttaatgt ttgggtttgc tgtagatgaa acaccagaat
      721 tgatgccttt accaatcgct ttgagtcatc gtctggtacg acgcttggca gatttgcgta
      781 aatcaaatga attaacgtat ttacgtccag atgcaaaatc tcaagtaacg gttgaatatg
      841 atgatcaagg acaaccggaa cgcgtagata caatcgttat ttcaacacag catgatgatg
      901 cagtagataa tgaaacaatt cgtcatgatg tcatcgaaaa agtagtgaag gaagttattc
      961 cagctgaatt attagatgat caaacgaaat attatatcaa tccgactggc cgatttgtca
     1021 ttggtggtcc tcaaggggat gccggattaa caggaagaaa aatcattgtt gatacgtatg
     1081 gcggttatgc tcgtcatggt ggcggtgctt tttctggtaa agatgcgaca aaagttgacc
     1141 gttctgccag ctatgctgca cgttatattg ctaaaaacat tgttgcagca gggcttgctc
     1201 gcaaagcaga agtacagcta gcttatgcga ttggtgttgc tcaacctgtt tcgatctcaa
     1261 tcaatacttt cggtactgga acggttccgg aagaagaact gattgctgca gtgagagaaa
     1321 acttcgatct tcggccagca ggaatcatcg agatgcttga tttgcgtcgt ccgatttaca
     1381 aacaaacagc tgcttacggt cattttggtc gtacagatgt agatttgcct tgggaacata
     1441 cggacaaagt agatgcgttg aaagcaagtt tagcaaaata agttatattc agctggaacg
     1501 atcgccaagt ggtggttgtt ccagcttttt ttatggcagt taacctcttg aaaaagatag
     1561 taattattta tctattttta gatcctgtca taatgaaagg gaggaataag atgaaaaaag
     1621 aaacaaatgt tactttggtc acagtgagta ttttcattgc aacgttcatg acggctattg
     1681 aaggtacaat cgtaacaacc gctatgccaa cgattgtcgg ttcattacat ggaatggaaa
     1741 tcatgaactg ggtattttcg atttatttgc tgacaaacgc tatgcttact ccgatttacg
     1801 gaaaacttgc ggataaagtg ggacggaaac caatctttat ttttgggaca ttattgttta
     1861 ttatcggttc gttattttgc ggtttatctc aaagcatgat tgggttgatt gctgctcgtg
     1921 cgattcaagg gatgggtgct ggggcaatga tgccagtagc tttgacgatc atcggcgatc
     1981 tttattctat tgaaaagcga gcaaaagttt taggtttgaa tagttcagcc tgggggattg
     2041 ccagtgtttt tggaccgctt gcgggaggat tcatcgttga aacgatcagc tggcactgga
     2101 tcttttttat caatgtgccg atcggaattc tattaattat tttgatttgg ctatatttaa
     2161 atgaaccaaa aatcatacat gagtctaaac caatggatat ttttggtagt tttagtttga
     2221 tggccatgct tgtctcattg ctgttagggt tccaattatt aggagacgaa ggaatgagtc
     2281 cgcttgtttc tgctttgctg ctaggtagta tcgtaatgtt aggtattttt gtttttgccg
     2341 aaaaaagagc taaagatcct gtcatcatga tgcatttatt caaaaatcca ctatttgttg
     2401 tggtcaactt ggctgcagca ttggctagtg gttttttgat gggaatcgat gtttatatcc
     2461 ctatgtggat gcaaggtgta ttagggctga atgcagcaat tggtgggctg gtattagcac
     2521 cattatcact agtctggatg gtcggttctt tccttgcaag caagtggatg actcaaatga
     2581 aaactcattg ggtcttgaga attggactac taatcgcagc tattgggggc gcttggttgt
     2641 atcttatgcc tttcagaagt aactatttat ggttttttgc tatatcaacg gtcatcggta
     2701 taggcttagg tgtgaccatg actacctcga ctgtcagtgc acaaaatagt gtatctcgtg
     2761 accagcttgg tgtagcaact tcttttaata cgttagttcg aacaattggt caaacagtca
     2821 tggtttctat ttttggttta ttgataaatg gggtaactaa acgagaactt gcagcagcaa
     2881 atttaacaaa tgatgcagat atcatgaata aactggtgaa tccacaaacg gctaaatata
     2941 ttgatgcgga tattttgtta ccattaagaa aaattcttta tgatgggtta cattccgttt
     3001 atctagttgg cttgttactc gtgatcattg ctttggtctt aacgcttttc gttcatgaaa
     3061 aaagtggtaa aataaaacag tagtttcggt taagatatgt acgtagccga atcattcaaa
     3121 tttaaagtta ttcaaaaata acttcttact aaggaaggcg aaaatatggc atttattcaa
     3181 gcaaatattt attcgaatgt gttagaaatg gaagtcaatc taaatgtgat actaccgcaa
     3241 acaacaaaaa aggtgattgg caccagttca gagcaagaga caacggatat acctgtttta
     3301 tatttacttc atggaatggg tggcaatcat agtgtatggg aaagacgtac gtcgattgaa
     3361 cgttatgtag caaactacgg attagctgtc atcatgccat ctactgatct tggctggtac
     3421 acagatacag tttatgacat gaagtattgg acattcattg cagaagagct acctgagatt
     3481 tgtcatgaat tattcccgca attaacaaga aaaagagaaa aaacatatgc cgctggatta
     3541 tcgatgggcg gatatggcgc gttaaaacta ggccttgcaa aatcagaaaa tttcgcagca
     3601 gtaatctctc tttcaggcgc tgtttctgta ggaaatcgaa tggatgatct tttgatggtt
     3661 cgtaaagcat ctttttggga agggatcttt ggaccattgg atcaaatcga aggttcaaaa
     3721 aatgatccgc aatacttatt gaaagaacta gtaaaaagcg gaaaaccagt tccaagattt
     3781 tatatggctt gtggggaatc tgattttctt tatcaggcaa atcaggaaat ggtatctgct
     3841 atgcaggaac aaggcttggc ggtcaccttt gaacaaggac ctggcgatca taactggatc
     3901 ttctgggacc aatggattca acgtgcattg gcgtggctaa tggaagaaaa ataaaaaaag
     3961 agtcttaaaa agatagctcc tatagagatc acaaacatag tgatctctat aggagctttt
     4021 ttactgctcg ttttctcttc ttttattttg ttggcataag ccttcaatat aggtttgtgc
     4081 actcgttaga aagagtgctt tttcattcaa ttctgcatgg gactgcatac tttctttaaa
     4141 tccctctaat tgattttcaa ctacataatt acgcataaat agaactgttt ctttcaaaaa
     4201 ttcatttccg tccataattt tttgtcgaag ctgctgttca tgctgctgat ccattaactg
     4261 tccaatcaat aaattgtgga gagaggcaat ggcctgatga ctttgggtga ctgaaaatcc
     4321 ttcacgaaca agtaattgga tcatttgctc caaatgttct aatcgactcg gaaaagcggg
     4381 cacagtattg atttctaatt ctgccccaca agggaaacgt gtataaaggt catagtaatt
     4441 ttccatatac tgcagcaact gttcattcca aggcaaagtt ggatctggaa gcgctaattc
     4501 ttcttccatt gtttcagcca ttttttgcaa gagctgttgt ttgtttttga aataccaata
     4561 taaagcaggt gcttgaacat gaattctgtt tgcgatgttt cgcatggaaa gctgcgaaag
     4621 agcttgtgtt tcagccagga tatcaaaggc ggcctgaatg atgattgctt gagaaagctt
     4681 tatttccatt ttatcaacct cgtgtattgc attatctttt taaacatggt aaattgttag
     4741 tcggtctaat tttacagtgt taaattagtc ctgtcaattt aaacaaaggg gtgtaatcaa
     4801 cgaatgaaat taatcttacg acatgcgaaa aaatataaag tagctgtttt tatttccttg
     4861 ctgtccgtag cagtcatggt tgcagcagca ttatggcaac ctaagttact acagcaagta
     4921 ttggaagcta ttattacaga agataatgat gaaatgaaaa caattggtat ttacctgatc
     4981 agtctggctt tgctaggatt agccgcaggt gtaacgaaca cgattttctc tgcaaaagta
     5041 gctcaaggcg taagtgcaga tatccgagaa gaagcatttc gaaagatcca gacattttca
     5101 tttggaaata tcgaacagtt ctctgctgga aatcttgttg ttcgtctaac aaatgatatc
     5161 acgcaaatcc aaaatttagt gatgatttct ttgcaatcac tttttcgaat tccattctta
     5221 tttattggtg ccttcatttt agctatgatc acgatgccgc aattatggtg gatcatcgta
     5281 gcgcttatcg ttacggtatt tctgattacg gcactttcat ttacaaggat gggcaagcac
     5341 ttcatgatca ttcaaaaact gattgatcgt gtgaatagca ttgcaaaaga aaacttacta
     5401 gggattcgtg tagtgaaatc ttttgtacaa gaagaaaacc aattgaagtc attcagcaaa
     5461 gtaagtgagg acttaacaaa acacaacatc atagtcggta cattattttc tgtgatgatc
     5521 ccgtccttca tgctggctgc caatttggct gtggttgggg cgatcttttt tgtgagcgat
     5581 cttgttaaag atgatcctac gttaattggt ggaattgctt cattcatgaa ctacttgatg
     5641 cagattatga tggcaatcat tattggcgga atgatgatga tgatgacttc aagagcagcc
     5701 gtatcgctga agcggattgc ggaaatttta gatgctgaac cagatcttac ttatttagac
     5761 gttccagaac aagagttgac gggttctgtc acttttgacc atgtttcttt tcgttatcct
     5821 ggtgaggata cagatacgct taaagatatc agcttttcta tcaaacctgg agaaatggtt
     5881 ggtatcgtcg gggcgactgg tgccgggaag tcaacactag cacaattgat ccctcgtcta
     5941 tttgatccga cagaaggcaa agttgaagta ggtggtgtag atcttcgtca agtaaatgaa
     6001 aagagtttaa gacaaacggt ttcctttgtt ttgcaaaaag caattttatt ctcaggaacg
     6061 attgctcaaa acttgcgcca agggaaaaag aatgcaacag aaaaagagat ggaacatgct
     6121 tcttctattg ctcaagcaaa agaattcatc gaaaagttat ctgatcgtta cgaagcacca
     6181 atagaagaac gtagcagtaa cttttctggt ggtcaaaagc aacgtttatc cattactcgt
     6241 ggcgttatcg gtaatccaaa gatcttgatc ctagatgaca gtacgagtgc actggatgct
     6301 cgctctgaaa agctggtccg agaagcactc gatcgagatt tgaagggaac aacgacgatt
     6361 gtcatcgcac aaaaaattgc ttctgttgtc aaagcagacc gcatcttagt attggatgaa
     6421 ggcagattag tcggcgaagg aacccatgag gaattggtag ctggaaaccg tgtctatcag
     6481 gaaatctttg aaactcaaaa aggaacggag gagtaagtat gactgatcta ataaaagcaa
     6541 gtaaattttt ctatcactat ttgaagcggt acaaactctc ctttttcttt atctttatca
     6601 cagtgatcat tgctacctat ctacaagtaa aggcacctca atacatcggg gaagcttttc
     6661 aagaattagc taattacgta ggaggcttga tgcaaggcgt agacgataaa agcaaatttg
     6721 tttctgtaat atggaaactc ttgctgtttt atgtactagc tagtgcagcc aactttatct
     6781 atagtatttt gttcacacaa gtagtaggga agtcaacgaa ccgaatgcgt atcggtctat
     6841 tcaacaaatt ggaaaaattg acgatccgtt tctttgattc tcatcaagac ggtgaaattt
     6901 taagtcgttt taccagtgac ttagataata ttcaaaatag tttgaaccaa gctttgcttc
     6961 aagtcatcac gaatgctgtt ttacttgtcg ggatcttgat catgatgttc cgccaaaatg
     7021 tagagttagc ttgggctaca attgcttcca ctccagtagc cgttttgata gctgtcttga
     7081 ttatccgcaa agcacggaaa tatgtggatc ttcagcaaga tgaagtgggg aaactaaatg
     7141 gttatatgga tgaaaaaatc agcggacaac gtgtcatcat cacaaatggc ttacaagaag
     7201 aaacgattga aggattctta gagcataatc aaagtgttcg cgaagcaacc ttcaaaggac
     7261 aagcttattc tggtctacta tttccgatga tgcaaggaat gtcactggtc aatacagcga
     7321 tagttatctt tttcggcggc tggttagcat taaatggtga tttggaacgt tcggtggcat
     7381 taggcttagt tgtgacattc gtccagtatt cacaacaata ttaccaacca ctgatgcaga
     7441 tttcatctgg atacagcatg atccaattag cgattacagg ggcaagacga ttgaatgaaa
     7501 tatttgatga aaaagatgaa gtcaaaccag aaaacggtaa agcatttgat ggtgttgaaa
     7561 aaggtcttgc gttgcagcac gtagattttg gttattctcc agaaaaacta gtcttgaaag
     7621 atgtctcgat caatgtggac aaaggagaaa tggttgctct tgttggtcca acaggatcgg
     7681 ggaaaacgac gatcatgaat ctgctaaacc gtttttacga tgtcaatgat ggttcagtag
     7741 tcatcgatgg aatagatatc cgagattatg atcttgatgc attacgtaga catgtaggaa
     7801 tcgttttgca agaatctgta ttgttctccg gaacgatcaa agacaatatc acattcggca
     7861 aaccggacgc ttcagatgaa gaagtcatca atgcagcaaa acaagcgaat attcatgact
     7921 tcatcatgac gttagaaaaa ggttatgata cagagatatc cgaagaaaat aacttattca
     7981 gtacaggtca aaagcagttg atcagtattg ctcgaacgat catcacaaat ccatctttat
     8041 tgattttgga tgaagcaaca agtaatgtag acacggtaac agaagcaagg atccaaaaag
     8101 caatggatga agcaatcaaa ggaagaacca gctttgtgat tgctcaccgg ctgaaaacaa
     8161 tcttgaatgc agatagaatc gttgtcttgc gtgatgggga agtcattgaa gaaggcagtc
     8221 atcatgagtt attagcagaa aatggatttt atgcggaact ttatcataat caatttgtat
     8281 ttgaaagtta aattaaaaga acctcgttat aacggggttc tttttatata ttttatgttg
     8341 tttgttttta taaggggca
//
LOCUS       DAFEPQ010000090         8026 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.092, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000090
VERSION     DAFEPQ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.092"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="LP543_002431"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="LP543_002431"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBL2322565.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="LP543_002432"
     CDS             complement(1098..2201)
                     /locus_tag="LP543_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBL2322566.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3247)
                     /locus_tag="LP543_002433"
     CDS             complement(2291..3247)
                     /locus_tag="LP543_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBL2322567.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="LP543_002434"
     CDS             3528..5462
                     /locus_tag="LP543_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL2322568.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="LP543_002435"
     CDS             5919..6335
                     /locus_tag="LP543_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBL2322569.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="LP543_002436"
     CDS             6349..6915
                     /locus_tag="LP543_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBL2322570.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="LP543_002437"
     CDS             6938..7924
                     /locus_tag="LP543_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBL2322571.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
BASE COUNT         2399 a         1557 c         1618 g         2452 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaatcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt cacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacaagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc aatcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaa
//
LOCUS       DAFEPQ010000091         8023 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.093, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000091
VERSION     DAFEPQ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.093"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            69..1706
                     /locus_tag="LP543_002438"
     CDS             69..1706
                     /locus_tag="LP543_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322572.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1851..2591)
                     /gene="truA"
                     /locus_tag="LP543_002439"
     CDS             complement(1851..2591)
                     /gene="truA"
                     /locus_tag="LP543_002439"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBL2322573.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2782..3417)
                     /locus_tag="LP543_002440"
     CDS             complement(2782..3417)
                     /locus_tag="LP543_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBL2322574.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3564..4805)
                     /gene="sstT"
                     /locus_tag="LP543_002441"
     CDS             complement(3564..4805)
                     /gene="sstT"
                     /locus_tag="LP543_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBL2322575.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5003..5999
                     /locus_tag="LP543_002442"
                     /pseudo
     CDS             5003..5999
                     /locus_tag="LP543_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
     gene            6015..>8023
                     /locus_tag="LP543_002443"
     CDS             6015..>8023
                     /locus_tag="LP543_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL2322576.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2631 a         1499 c         1244 g         2649 t
ORIGIN      
        1 gacttgactc actagtatga tatactagaa aagaatcaat ttttattact tttaaatagg
       61 agaaaaaaat gaaaaaaaac aaaaaccaaa taatagatat catttttatg ttatttctaa
      121 gttttgctta cgtcatccct ctattcatgt ctatgggaat ctatcatagt gtcaatcagg
      181 atacttactt tcatttatct cgaatcatag gactagataa tgtatggagt tctccagtaa
      241 acttcaataa cttcgatcat catggaacaa tgatgaatat tttttatcca tggcttactc
      301 tttatccagc ttttttattt tataaaatga tgggaaatct tgttttaagt tataatatct
      361 actatttttt tatcactttt ttaacaatgg tcgtttctta tttttctatg aaacaaataa
      421 aaaacaatcg ttatatttcg ctgttatttt cgattattta tacattttcc gcctatcgtg
      481 ccattgacat attccatcgt gcttcattgg gagaagctgt cgctttaaca tttttgcctt
      541 tgatactaat ggggtgttac gaaatttata ttagagatta ccaaaaatgg tactggttgt
      601 ctattggcat gacgctagta gtgtatactc atttactttc tgtcgctatg gtttctgtat
      661 ttattggagg aacgttgttt ctttcttttt atttttggga ccaaaaaatc gctagactct
      721 taagtctttt aaaagctacc gtcctcacct tttttttaag tgcgggattc ttaattcctt
      781 ttattcaaca atctagagca caagaattga aagtcccact gggaaaagaa ttaagtggta
      841 tggctcctag tgatatgctg acacacattt taaataataa ctataacaac tatactattg
      901 gattattctt atttctcgga ttgattggtg cttttatttt tataaaaaaa ttaactgctg
      961 atgatttatt tattttcttt cttggtgtat ttgttctttt ttgttcaacc aatttatttc
     1021 cttggcaact ttttaaccat acaccagtta agagtctaca atttgtctgg cggttaaatg
     1081 gtttttcttc tttatttata gcctatacga tgagtatagt gatctattat attttttcta
     1141 ctaaaaataa ttcatggaaa ataatggtct ttacgtttct tgcactaata ttgcatctct
     1201 caggagtaag caattctatc catgctaaca aagattcatt aacacttata gcacctgaag
     1261 acgctgttgc tatagctgaa aattacaatc atactgatta tgcaaacaaa gagtcaatct
     1321 atcatcctga tatgattaat aatgatcaat atttactagg taaccaggaa ataaatccaa
     1381 caactcattt tatgagtaac aaacttacaa ttgaattaga taattcgaat aataaaaata
     1441 ctgtcttgac gacacctatt tatcgttata aagggcaagt ggcttctatt aatggcaaac
     1501 tagtacaaac aaaattatct aagtttggca caactgaatt aactattcct ccaggtataa
     1561 ataaagtcgt tattacttat cagtacacaa aactagcaat tgcttcaaga tatttatcta
     1621 tcgttacttt gattttattt ttactttatc gatttacttt ttctaaacaa aaacaaccga
     1681 gaagagaagt acaacattct cactagattt ttatttaaga aagacttttt agctgtacac
     1741 ataaacaggc taaaaagtct ttttttatat ttctttaatt tgcttcatac attatttaac
     1801 atcgatattt atactgtgaa acatgcgttt catccattat caaagtattt ttatcgataa
     1861 taaacttcat ccaaaaacag accttgtgca gggacggttt ctcctgcatg ttgccgcact
     1921 ttgctttcaa atatttcatc gattgtttct atcggcatgg cacctgtgcc gatttctagt
     1981 aatgtcccca tgataatccg caccatttta tataagaatc cgtctccgac aaaagtaaaa
     2041 tgaagcattt ctccctttcg ttcgatagat aattcatcaa tcgtacgcac tgtggatttt
     2101 tttgattttt tcaatgcaga aaaaccaatg aaatcatgtg tacccactaa tttttcgcaa
     2161 gctgcattca gcttgtccat atccaactct tgagggaaat aaaagctgtg gtttcgctca
     2221 aatgctgaag gaatcacgtt gttccaaaca taatagctgt attttttgcc caccgcatta
     2281 tatcttgaat ggaagcgctc aggcatttct tcgattcttt tgatgacaat atcccgcgga
     2341 aggtaacgat tcataaagtc gatcatttct gccagtccaa tttctgtgtt tgttttaaaa
     2401 tttgctactt gtcctcgcgc atgggtgcca gcatctgttc ttcctgatcc aataatttct
     2461 atctcttcat ttgtcatctg atgcaatact ttttcgattt ttccttgaat ggttttatcg
     2521 tcatctccaa gtctttgcca tcctgaatat ctttttccgt catattcgat tgtcaacttg
     2581 atgtttctca tattttagtg gctcctctgt cttccatttg gtcttctctc taccttttta
     2641 agattgtagc acaaaatata gctcctttct tctctaaaac agaagctata caagaaaact
     2701 gtggaaatat atatttcaga aaaacaaaaa acagccaccg gatgaagtat ccgctgactg
     2761 ttcaatttga cggctcaagt tttactgttt gagatgcaag atactcaacc aggcaatggg
     2821 cacataatta aagaaaaaac tgattctttg gcaaaaacct attttattca gtaatggcca
     2881 agaatttata gaaggaattc ttgccagtcc ataaatcgcc gcaaaaagca aactgatcac
     2941 agacagtcca aggtataagt ggcttacgtc catttgccca ttttttcgat agatcaagaa
     3001 aagaagaact ggaaacaata aaaaaccact gtaacctaaa cctgatccga tattatggac
     3061 ccatgtcgag acattccacg aagattcatg ggtattgata ctgaacaaac ctgtaaagat
     3121 acaatcacct ataccataga acgcgatggc tgctgtaact agggtagcta acacacgaga
     3181 tgtacttaca aatgcagcat acaatgcagg caatgacagg atgaaaaaga tccctgaaac
     3241 cacagaccaa ataagaaacg cttttcttac aggactggaa acctctccaa atacactgat
     3301 cacgctcttc atttgatcta attcaggata atacaatcct aatacgtacg gagtcaaaaa
     3361 atcactcagc acgcctaata ataaaaagaa aaaaccaaat ttacgtaaaa aatccatgat
     3421 acgcctccaa taacaattac ctatgctttt aattgttatt ataagcgact ttatcttgat
     3481 ttaacagaaa cctctattta cacgaacatc tatataactc attaaaaact cgaatcattt
     3541 taaaaatact aaatatctaa cgttcaacgt tcacttaatc catctaaaca atctttcaat
     3601 ttgatttttt ccccacttct tttacgcgcg cctagctctc ctattgcagt aaacaacaga
     3661 tcacttgacg aatttaaggc agtttccatt gaatcctgga taacaccgat aataaatcca
     3721 ataccgacga tttgcatcgc gacatcatta gagataccaa acaaactaca tgctaatgga
     3781 atcaataaca gtgaaccgcc cgcaacacct gaagctccac aagcagctaa agcagacaaa
     3841 atacataaaa ggactttcaa aacaaaaggt gcttcaatcc ctaaagaatg tacagcagtc
     3901 aacgtcatca aggaaatggt aatcgcggca ccgcccatat tgatcgtagc acctaacggg
     3961 atcgatatgg aataagactc ttttgtcaat ttcaatcttt cagccaacat catgtttatc
     4021 gggatatttg cggcagaact tcgggtaaag aaagcgggaa gtgcgctttc tttcaaacaa
     4081 aagaaaatca atggataggg attttcttta ataaacaaaa acgtgaggaa tgggtacaca
     4141 actaatgcga caaacagcat cgtaccgatc aaaacaagta aaagctgcag atactctgcc
     4201 aatccagcta ttccagtcgt agcaattgat tgataaacca atcctaaaat ccctatcgga
     4261 gcaaatgcaa taatcacttg tacgacttgt gacaacgcta tagatatctg atccaccaac
     4321 tcttttgtcg attctgaacg tgctcgaaaa gctagtccca acaatacagc ccagaataag
     4381 acgccaaggt aattcgcatc aataagagat tgaacaggat tttgtgtgac attcgtcaac
     4441 atagtagata aaacactttc caaactttgc ggtgcttttt cagatactgt ttcttgtaaa
     4501 gtaataggaa cagtaaaaat tgaacttaca ataactgcta caagtgcaga aagcaatgtt
     4561 cccactaaat ataagatcaa aataggcttc acgaatgtct tattcccaat tttatgcttg
     4621 gcaattgacg ccatcgtcaa gaaaaaaacc agaataggtg cgattgcctt caagcaactg
     4681 acaaacaacg ttcccaatat tgaaataaat gtccatgtgg ggaaaaagat accaaacaat
     4741 attccgacta aaataccaac aacaatacgt tgaactaacg acatatccaa aaacttctct
     4801 aacaaatgac caccctttca caattttaac aatcattgta tcattttatg caaagaaact
     4861 aaaggtataa ttttctaaaa attctaacaa tacaaacaac catttcttgc taattttgta
     4921 ctttttctgt ctacacgttg ttttttgaga tattctaagt taggatgtta ttaatgggca
     4981 acttatcagg aaacgttttc ttatgttttt gtccaatttt tttgtgaggt gtttattctt
     5041 gaaaaaaata aagaaggaga gttatggctt cctgacaatc tttgtcgctt gtataatgat
     5101 ggttttacag cgtatattga tttcagcagt cactattcct acatatgcaa caaaccctgg
     5161 tcgattctac atctatacat ttttacaaac ttccgttgtt attggtgcaa atttgatccc
     5221 actttacatg ggttaccaat atcaaaaaat cgcccgttta aaagttttac attatttaag
     5281 tcgttttgcc tttatattcc taatagccac actagtttgc aatatctttt ttttacgtac
     5341 aagctggcag cttgaatatt cgtgattatt ggcttatcct aacacctatc agtcagaatt
     5401 actttactta tgctgtttct tgtactttgc ttttttgtag tattccttat acagttggat
     5461 ttttaaataa attatcgaaa acaacaatta aatatctact attattttta acaattggat
     5521 tagttggctg ctctactcta tttaataaag acccgtttgc attacagaat ggacagagta
     5581 ttttatggat tttttattta tttttaatgg gttatggttt gaaagaatgg aactggaaag
     5641 aaaaggtccg ctataagctt cctcaagtat ttatcagctt gattgtttta ttcggtttga
     5701 ttattttgat gacacaaatt tcactcatca tcagaggaaa tacagataca gcgctgaggt
     5761 tttctaaacc atattctctc ttcgcaatgt attatacttt ctcttcgttt ttatttttag
     5821 aaatgctatc tgataaatta ggtataaaag gaagaggaaa ttttcttgct gtatttttga
     5881 ttgtcactca agttatatgt aatttccctt tagtttctta tcgcgtatct acatttatga
     5941 aacgtgaatt tccaaactca gggaaggcat ggctgataaa tatcgggcag ttttttattt
     6001 gctatttatt agcagtgtcc ctcctagtta tctttttatt ttatatacaa aaaatccgtt
     6061 tcattcatcg tttcatcgaa tacttcgctt ttgattcgat tgaacaatta actcagcgtt
     6121 ggaaaaaaag aattcattgg ctttttgttc ataaaagtta ttttttagtt gcagcatttt
     6181 tttactgctt taccttcgtg cagattttcg ttttagaacc aaaagaaggt tggaaagaaa
     6241 ctatccaggt tgccttaaaa atatttactc agcgacaagc acccatgatc ctgacaatca
     6301 tcattattat gggatttttc ttcttactat tgttgatttc caatcgtttt tggtacgcct
     6361 tgactgctac cttgatcatt aacttactgc tcactatttc gacagtaatc aaaatggaaa
     6421 tgcgggaaga gcctgtcttt ccttcagatt taaaaatact gaccggattg tcagaactat
     6481 tatcgatggt tagcccagtc ttattaatcg tgggcggatt gatccttctc ctattattga
     6541 ttactagtat tattgtacaa cgtagactac aaaaacagta ttccttgaag attcattgga
     6601 aacgtcgatt tatcagtatc gctgtattat taggatgttt ttcgggtgtc tttttcatta
     6661 atcacaaaaa ttcgccttct ttcttgctgt ttaatttatt taaagtaaac aagactttct
     6721 ttaaccagca aggcgccgta aaagataacg gaccagtcat tcaattccta aacaatatcg
     6781 acattaaaat catggaagaa cctgctggct attcgaaaga aaaaattaat ggaataatga
     6841 aaaagtacaa cacaaaggca gatgaaatca accaaacaag aagtgactgg ttaaacaatc
     6901 agacgatcgt tctcaattta agtgaaagct tcagtgatcc tgcacgtgtc cctaatctga
     6961 cagtacctac agaccctatt ccttacatca aaagcattat aacagaagga acaggaggat
     7021 tgatgttatc cgtcggttat ggtggcggaa cagctaacat ggaatgggaa ggactgacag
     7081 gcttgagtat cagtaacctg tctgcttcgt tagttacacc ttatacccag ctagtggagc
     7141 gtcagaaaat aagtccaaat attaccgatc tattcgatga aaaaatagcc atccatcctt
     7201 ttacagctgc tctttatcgc agaaaacaag tgtttgataa atttggtttt gacaggttct
     7261 attatttaga cagtccggat aaattaactt atacggataa aatagaacaa agcccacgca
     7321 tatcggatga ttcagcatac aaagaaacct tgaaagcttt acaaagcaat acagaaacgt
     7381 cacaattcat ccaattatcc actatgcaaa accatatgcc ttatgatgat tactatagtg
     7441 aattgaacta cactgctgaa ggaactgctg tcattcctaa tagaagaaaa gaattacaaa
     7501 cttatatgca aggccttcac tataccgata cagcagtaaa aaaattcatc caagaaatcg
     7561 acaaaatcga taaaccgatt acctttgttt tttatggtga ccatttaccc gcaatctata
     7621 gtgggaatga ttcgaaaaaa tatggattag aacagcacca gacagattat ttcatttata
     7681 acaacaagta tagtcgtgaa caagcagcta cgttaaataa agaagttgtt gctcctaata
     7741 actttggtgc tatggctctt gagcatgcga atatcaaagt aactccttac tatgcgttgt
     7801 tgacagaagt atctaatcat ctacctgcat taacaattga tcctacagaa agcttgtcta
     7861 atcgttttaa tggaaaacag gtctttgtta cagaggatag tcaaattatc caagaaaaaa
     7921 atttaactga gaaacaaaaa gaaattctag aagattatcg tctgatacaa tatgatcttg
     7981 ttgcaggtaa ccagtacagt gcaacatggg cgacacaaaa aat
//
LOCUS       DAFEPQ010000092         7623 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.095, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000092
VERSION     DAFEPQ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.095"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(14..706)
                     /locus_tag="LP543_002444"
     CDS             complement(14..706)
                     /locus_tag="LP543_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL2322577.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="LP543_002445"
     CDS             complement(1143..1712)
                     /locus_tag="LP543_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL2322578.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="LP543_002446"
     CDS             complement(1786..2109)
                     /locus_tag="LP543_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322579.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="LP543_002447"
     CDS             complement(2263..3075)
                     /locus_tag="LP543_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322580.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="LP543_002448"
     CDS             3429..4277
                     /locus_tag="LP543_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBL2322581.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="LP543_002449"
     CDS             4422..5363
                     /locus_tag="LP543_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HBL2322582.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="LP543_002450"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="LP543_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1511 c         1371 g         2469 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aac
//
LOCUS       DAFEPQ010000093         7595 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.096, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000093
VERSION     DAFEPQ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.096"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(940..1353)
                     /locus_tag="LP543_002451"
     CDS             complement(940..1353)
                     /locus_tag="LP543_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBL2322583.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1809..2294)
                     /locus_tag="LP543_002452"
     CDS             complement(1809..2294)
                     /locus_tag="LP543_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322584.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2433..2678)
                     /locus_tag="LP543_002453"
     CDS             complement(2433..2678)
                     /locus_tag="LP543_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBL2322585.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2870..3670
                     /locus_tag="LP543_002454"
     CDS             2870..3670
                     /locus_tag="LP543_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL2322586.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3689..5557
                     /locus_tag="LP543_002455"
     CDS             3689..5557
                     /locus_tag="LP543_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBL2322587.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRSYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5550..6041
                     /locus_tag="LP543_002456"
     CDS             5550..6041
                     /locus_tag="LP543_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBL2322588.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6029..6583
                     /gene="srlA"
                     /locus_tag="LP543_002457"
     CDS             6029..6583
                     /gene="srlA"
                     /locus_tag="LP543_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBL2322589.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6601..>7595
                     /locus_tag="LP543_002458"
     CDS             6601..>7595
                     /locus_tag="LP543_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBL2322590.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1214 c         1442 g         2232 t
ORIGIN      
        1 gttactatga ataacataat aagaaagtaa agtggattga tttgagatct ttttatactt
       61 caaaaaagat aacacccaac tttcaaaaat atgttttgga agttgggaca aacgagttaa
      121 tacattttaa tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga
      181 gagatcataa gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc
      241 catatttctt caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc
      301 cagcaccatt tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga
      361 agtaaaaaca aagctttact cataactaac aaaataccag ctccttttat ttttttctcc
      421 cccacattca aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta
      481 tgaataacag ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa
      541 gtttattttt ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag
      601 ccgatttcgt agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta
      661 tggataaaat agatcatcgg acaagcagat aagagtaagt atttgttagg ccttttgaat
      721 aagtaaaaga aagcactggt cattatgata aatggtatcc aaaacaaaag tttacttaag
      781 acaatcaaat gatcaactcc catatttttt accaagagag atcataacat ctttatcgta
      841 aaaacaaaat atttttttgc tcattttgaa aatttaaaca tactcgttct tatccaaata
      901 tataaaaatc gcccctcatt catcgagagg cgataagttc taatttaact caattttttt
      961 tgtttctttt ggctgataag ctgttttagg taataccaat ttcagcacac cgtttttaaa
     1021 ggttccagtg attttttctt catcaacatt aggaagatag aatgaacgtt tgtaagagac
     1081 cgaatgtctt tctttccgta catagttgcc atcttcttta tcttctgtat gggactcatg
     1141 attggcggag atcgtcaaag tattatctga atattcaact gtcgtgtctt ctttgtcaca
     1201 gccaggcata tcagcagtta gtttgtattc tttttcattt tcgactaaat ctacttttgg
     1261 ataacttgaa acacctaaaa aatcattgaa tgctggacta aacacgtcat tgaaatcagg
     1321 gaacatgtcg cgaatgctag gtacatttgc catttttgat catcctttca ataagtattt
     1381 tttatttatc cctatttacg taataactct attttgaaaa aaatgcaagt ataatggtta
     1441 gctttgtgaa tatttcatct aatacgatac aaaggctatc tttctaattt gaaaaaggga
     1501 tcattaaaat aataagaaga aaatatgtca aaaggtaaaa aagtaaattc ttctatccaa
     1561 atatagaaaa attgaatcta gaaaaccgcc cgacaataaa tttcattaac tcagttgacc
     1621 gaaaatgaag tagataccaa aaatttatca agagagcaag tagagaagtg agtatattca
     1681 taagagaaat agctgtaacg aattattagg ttaatgcaaa tgcttattta tactccgtat
     1741 aactttctca gaaaaaatga taccaataat gacttgctag tactgtaaat ccgtataata
     1801 tcatctaatc aaaatggtgt tccatttgca tctgtcatcg taatcgatcc ttcgggtgac
     1861 actcgataaa aaccaacgct tccagatcct ccttgggaac ggatttgttt gctggaagct
     1921 gtgaacaaat aatctcgatc aattttttga taaaagctaa taacaatatc gtcactttgt
     1981 aattcggaac ggtgggcaat aagaaataga agtgctttgt cagagttttc taatggaaga
     2041 gaaggtacag tgctttcttg catttcagat gttgaagaaa tgctaaacga ctgaatactt
     2101 gattggctat gttgcgaatc tacattacca gtagaagtat tagtaatgga agtagtagaa
     2161 gtacttttag ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc tttaacagaa
     2221 ttctttgttt tatatccaca ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa
     2281 atccctttat gcatgatagc tcctcctaaa atagattatc tttattcagt atatcactta
     2341 aacaaagaaa agcagatggt ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg
     2401 aagataataa cttttttaat aattatagct ttctaatatc tttcatcaac ttttctagta
     2461 atcattggga aaaatttcaa gcagtagatc gcacaaatac ctacgacgat ataaatgatc
     2521 ttcgcaaaaa tagttgttga accaccagca atcatagcta ctaaatcaag ttcgaacaag
     2581 cctaccaaca gccagttcaa ccctcccaca atcaataaag ttaatgcaat agcatctaag
     2641 ttttcattag aattctcctt tcttaaatga taaaacaatt atatatcaaa aagagaaaca
     2701 ttgcttatat aacgctcagg aattttagca atctatgcaa aaagatgaaa attttttttg
     2761 ctcttccatt agtacacatt ttttagctga agctgtaaat ttcatgtctt aattttttta
     2821 gtgagctttt atactggatt tgtaagaaaa aagaaggaga ggatcacata tgtcggattg
     2881 gttggatatt gcaggaaaag tagtaattgt aactggaggt tcctcaggaa ttggaagaag
     2941 tatcgtcgaa aatttgttga agcagaatgc acaagtagcg aattttgatg taactgaatg
     3001 ccgtatacag catgaaaacc tactatcttt aaaagtcgat gtgtcttcta aaacggacat
     3061 agaagaaggg atatataaag taatgaaaca tttcaagacg atcgatggtc tagtcaataa
     3121 tgcaggaata aatattcctt ctcttctaat agatagaaat catccaaaaa gcaagtatga
     3181 attatcagaa caagtttttg ataagatgat cgctgtcaat caaaaaagtg tatatttgat
     3241 gagccaagca gttggaagaa tcttggtgca aaagggatca ggagtgatcg tgaatctttc
     3301 ttctgaatca ggattggaag gatcagaagg acagagctgt tatgctgcta caaaagcagc
     3361 gatgaacagt tttactcgtt cttgggcaaa agagttagga aagaggaatg ttcgtgtggt
     3421 aggtgttgca ccagggatac tggaagaaac cggtttgaga acacaagaat atgaagaagc
     3481 tctttcttat acgagaggta taagcgtcga acagctgaga aacggttaca gtcgaacaag
     3541 tacgatccct ctaggaagga gtggtaagct gcaagaagtg gcggatctag tctgctatta
     3601 tctttcagaa agatcaagtt atatcactgg agtcacgact aatatttcag gaggtaaaac
     3661 aagaggataa aggatagggg ggacgcacgt gacgctagta aacagatggt accaattatt
     3721 gaaattacta gtcactcaca agaaagtttc aatccaggaa gtgcaaaaag aaatcaaaac
     3781 cagtaatcaa accataaaaa aagatattgg ggagttgaat gaagaaattg ccggtattgc
     3841 aaaaatcatc caaaaaaaca aacattttga acttgaaatt tatgattttg atttttttga
     3901 tgagatcatg caaggaaaat tgaaaacagc cagtgatttc aattcggcat ccaaaagaat
     3961 cgcttattta ctaaataggc tgatcgaaac agaggatttc ttacgtatgg atgatttgtc
     4021 tgaagaagtc ggtgtcagca gaggaacagt ggtcaaggat ctaaagacat taaaagagat
     4081 gatcaatgat tttgatgtaa agattacagg cacaccaaat aaaggactaa gaataatagg
     4141 agatgagcta gaattacgac tacttttatt ttattttgtt tctccctact tttcagagac
     4201 gaaaacagaa cagtttctag aagaaaaaca agcaatcaaa cattttcaga agaagacgaa
     4261 tgctagtaaa caagaaatca cccttttaac gaaagttatc ggtatttctt tgaatagaat
     4321 ctctagtaac tatctgttga agaagcccat caatcgttac agtgggtgtt ttgaatatat
     4381 ccaagaattc aatgaactga tcaatcagtt ggaagaaatc tatgaactaa ctttgagcca
     4441 atatgaaaaa gattttatca gttttccttt gaacatcatc aacacaagta taacgatgat
     4501 aaggaaagct aactctgatt tgcgcttata tttcgacccc atgatgatgc gtattcgacg
     4561 attagatctc attgatctgg atgagtcctt cttatatcaa gagatgaaac agcacctctc
     4621 tttattgata aatcgggtaa tttttcgtta tcgattgact gatttatttt atggtgagat
     4681 agaaaaaaag tatccttttt cttaccaaat cgctagcgaa tgcatacaag cattagaagc
     4741 tatcttgcaa cataagatct ctaaagtaga gatcagctac ctaacgttgt atttggagtt
     4801 agcatttcga tatcaaaaag agcatttttc ctctaaaaaa gtagccattg ttcatacgac
     4861 aggaaaagga acagcattaa tgatccaaag acaaatcaaa aggatattgg ggagggatat
     4921 acaaataaca catgtttcag aagaacaata tgaaaaagta gatctaaaca aatatttcgc
     4981 tgtttttaca acgattccgt taaaaaatgt aaatgaagag acaccggtga tccatttgac
     5041 taatcttttt gatgactctt ggttaagggc acaatggcaa aaagcaaaaa aaaagcgatt
     5101 agccgggttt cattctactg tttttttcta tcaaatatgg aaagaaaaaa agacgtatca
     5161 agaatattta aacaaattag tcgctccttt gatcagaagc tattatgtgg atgaagaatt
     5221 tgaaaaaagg atcttgaaaa gagaatatca gcaaagcaca tttgttaaca aagaatttgc
     5281 ttttcctcac acaaccaacc agctatctca acacattgtt ttatcagtag ggctaataac
     5341 tgatcaaggt ttagcagaaa caggaggaaa tggagttatt tttttatttg ccatacctga
     5401 agaaatgacg gctgaagttg agaatgaatt atttgagcta tacgatatcc tatttgaact
     5461 cataaaaatg ggaatagatg agtttattga attagcaccg acacaagaat catttttaaa
     5521 actaatcaaa agtaaaggag agagacttca tgaatgatat gtatctattc atagttttcg
     5581 cagcttcagc ttatgtgctg caagcagtta tcagttggaa gcaactaaag catttcaatc
     5641 aaacatatag cgaactgcga agaaaaggaa aagtagcgat tggtaaacga cctgggaaaa
     5701 tccgttcagg cacgattgtt atgctagcta tagacgatca tgatcaagtt ttagaagtca
     5761 gaaaaatgca aggggttaca tctttggcaa aatttcattc attacctgtc tatgcgggtg
     5821 aagatatcca ttacatcgat cgttaccatc cctcagttcg agcggaagat cgtttgacac
     5881 aagcagcttt agaaaatgcc agagaagtat acttacgaat agaggcagga aatgaaaaaa
     5941 gtgaacaacc ttcattgcct tttattgcta tgctcaataa ttttcggata tggaaaagta
     6001 cgatcaaaga aaaaaggagt gacaaacgat ggatatcata acaaaatttg ctgagggatt
     6061 tatgaggctg ttccaaacag gtgcagaaac attcataagc tggatgacag gaatcgtacc
     6121 agttgtcttg atgctgatgg ttgcaatgaa tacgctgatt gcttttttag gtgaagaccg
     6181 agtaacgaaa gtagccaaat tatcagcgaa aaatccgctt actcgttacc ttgtgttacc
     6241 gttcttatct gcttttatgt tgggaaatcc tatgtcattt acaatggctc gtttccttcc
     6301 agaatactac aaaccaagct attatgctgc tcaagctcaa ttttgtcata cgagcaatgg
     6361 tgtattcccc catattaatc ctggagaatt gtttgtatgg atggggattg ctcaagggat
     6421 cgaaacctta gggctaaatt cgatggagct ggctatccga tacttgcttg tgggactgct
     6481 gatgaacttt attggtggtt ggataactga ttttactact ggatttgtat gcagacagca
     6541 aggaatcgtt cttagcaaaa aagtagagct ttcagttgat tagagaaagg agagttttaa
     6601 atgtatggtg atattaagat cgtaagagga aacggcgggt atggaggtcc tttgattatt
     6661 aggccgacaa aagataaaca caagttcatc tatgtcacag gtggaggtga gaggccaatg
     6721 atcgtagata aaatagaaga actgacaggt atggagtcta tcaatgggtt caaaacatca
     6781 atacctgata gtgaaatcgc gcttgcaatc gttgactgtg gagggactct tagatgtgga
     6841 atttatccta aaaaagggat accaaccatc aatatcgttc ccacaggaaa aagtggccca
     6901 ttagcacaat atatcactga agatatttat gtttcagcag tagggttaga acaaatacag
     6961 ccactaatgg aaactgatga taacaaagaa acagtaaata ctgcatctcc agataaaaag
     7021 tacaaatatg atacttctaa aaagataacc gagcaaaaag cagagcagca aaactggata
     7081 gcaaaaattg gaatgggcgc gggaaaagta gttgctacat tcaatcaatc agcaagagaa
     7141 gcagtagata cgatgatccg gacagtgatc ccttttatgg ctttcgtttc ccttttgatt
     7201 ggtgtgatac aaggttctgg gatcggtaat gtatttgcta acattatggc acctttagca
     7261 ggaaatgcag ttggattaac aatcattggc tttatttgtt cactgccatt tttatctcct
     7321 ttgcttggtc caggggcagt tattagccaa gtgattggaa cattgatcgg tgtagagatt
     7381 gggaaaggaa atattcctcc ccaattagca ttaccggcac tatttgctat caatactcaa
     7441 aatgcatgtg attttattcc agtcggtcta ggtttggaag aagcaaaagc tgagacagta
     7501 gaagtaggtg taccatcagt tctttattca agatttctta acggcatccc acgagttatc
     7561 attgcttggg tagcaagttt tggattatat agctg
//
LOCUS       DAFEPQ010000094         7395 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.097, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000094
VERSION     DAFEPQ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.097"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(2..454)
                     /locus_tag="LP543_002459"
     CDS             complement(2..454)
                     /locus_tag="LP543_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBL2322591.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPVEKEKPKKKKSFWDRLF"
     gene            604..1554
                     /locus_tag="LP543_002460"
     CDS             604..1554
                     /locus_tag="LP543_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBL2322592.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            1547..2506
                     /locus_tag="LP543_002461"
     CDS             1547..2506
                     /locus_tag="LP543_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBL2322593.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            2503..3258
                     /locus_tag="LP543_002462"
     CDS             2503..3258
                     /locus_tag="LP543_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2322594.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIIAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            3281..4234
                     /locus_tag="LP543_002463"
     CDS             3281..4234
                     /locus_tag="LP543_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL2322595.1"
                     /translation="MKKLVVGILILASFGLAACGNSDNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(4878..5174)
                     /locus_tag="LP543_002464"
     CDS             complement(4878..5174)
                     /locus_tag="LP543_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HBL2322596.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            5530..5886
                     /locus_tag="LP543_002465"
     CDS             5530..5886
                     /locus_tag="LP543_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL2322597.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            5896..6795
                     /locus_tag="LP543_002466"
     CDS             5896..6795
                     /locus_tag="LP543_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBL2322598.1"
                     /translation="MGKLAIISDLHVDIKKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYEPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(7028..>7395)
                     /locus_tag="LP543_002467"
     CDS             complement(7028..>7395)
                     /locus_tag="LP543_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL2322599.1"
                     /translation="IDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAI
                     YFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRKSLDYR
                     TPLEVFLSYVSIDDLSNLI"
BASE COUNT         2260 a         1269 c         1475 g         2391 t
ORIGIN      
        1 tttaaaataa acgatcccag aagctttttt tcttttttgg tttttctttt tcaactggcg
       61 aagagttttc ttctgggtat tttttttcga tatcaatcac aggttcattt actgcttctt
      121 ttgctgttaa gagcaatccg aattcctcag gatttgcagt gacgtaatcg ttgactacag
      181 taaatcggct attgtttttc gttgctagct ggatatattg gttttggaca gaaaggggta
      241 gagccccatt caagagtatg gaagcatctg gatgtttttt gagttctttt aaaaagtttt
      301 ctttgttttt tggttggatc atttcagcaa cagtcatact aagatagcag cgttccctga
      361 atgtccctaa atatttatgc tgttcttcag ggttcactaa aggtgtacca taaagtccct
      421 tatccaaatg gctttttaca tcatcttgtg ccataaaaaa taccctcctt cttctctttg
      481 tctttcttac gattatagca tattcccaat aatacctaaa acatatgaaa aagcggttgc
      541 ttttgttcaa cgattcactt aaaataagaa tgaaaatcat tctcaattag aaaggtggat
      601 tggatgcgct acggacgact agtagcatta ttagttattt taagtagtat ttcattgttc
      661 atcggagtat cgaatgtaca attgtcagaa ctgctccagt tagatgggca aagcttacaa
      721 atcttgctag tgactcgttt gccacgaacg atttgtttga tccttgtggg agcgaccagt
      781 agtatttgtg ggttgatcat gcaacatttg acccaaaata aatttgtttc accaacgact
      841 gcgggcacga tggatagtgc gcgtttaggt attttagtgg ctatgatttt cctacctggt
      901 gcatccttga ttgtccgatc cttgacagca ttttgctttg cttttgcagg aacattgctt
      961 tttttatccc ttacgcgatt gttcccccag aaaaatcaat tgatgcttcc gttgattgga
     1021 gtcatgttag ggaatatcat tggttcagtt gctacttttt ttgcttatca attccagttg
     1081 gtacaaaata tgacttcatg gcttcaagga aattttgcga ctgtgatacg aggaaattat
     1141 gagttattgt atgctgttgt tccattgttg ctcttggttt ggctatttgc ttaccgattt
     1201 acgatcattg gtctgggcga atcgtttgca gtaaatttgg gaatagatta ccagaagatg
     1261 cagtttttag gcattggctt agtatcattg gctagtgcag tcatgttgat catggtagga
     1321 agcattcctt ttatcggagt gatcattccg aatcttgtct ctagaatata tggagatcaa
     1381 gtgcacaaaa caattggcgt aactgcttta gcaggtagtt tgttcttagt attttgcgat
     1441 cttgtcgctc gcgtgttgat ttttccttat gaaataccag tcagtgtgat cgttggaatc
     1501 atcggtgggg ctatcttcct ttatcttctg ataagggggc gacgatatgc ttaaaaaaaa
     1561 gccatctatt tttattggta tgcttgttct tgttttattt gttacaggaa tgtacttgac
     1621 gatccataca tatggaaact ggcattttgc atgggcattg aggagtaaga aaatttttgc
     1681 atttatcctt gtctcgctag ctgtcagcag tgccacaatc agctttcaga caattacgca
     1741 taaccagttt ttgactcctt cagtattggg acttgagtcg ttatatgtcc ttgtccaaac
     1801 ggtccttttc ttctttatcg gaggagtgac gatgcttcag cagacaggta cagttttttt
     1861 cttattgaac gttgggctga tggtcgggct gagtctattg ttatttttct ttcttttgaa
     1921 aaaaatgaga gcgaatcttt ttttattact gatgtcaggg atgattttag gtacgctttt
     1981 tggtagtgtc agtacctttt tacaagtcat catggatccg aatgaatatg atttgcttca
     2041 aggcagatta tttgcaagct ttggcaatat ccagagtcag tatcttttgc ctgcttcttt
     2101 gatcattatc ggctgctggt acctgttatt tcgatatcga catgtgctag acgttttcca
     2161 tctcggaaat gaacaagcag taaacctcgg gatagacaca gatcgttttg aatttttgat
     2221 tttatgttgt gtcagtcttt tgacagggac atcaacggct cttgtgggtc cagttacttt
     2281 tttagggttt attgtcgcaa atatcagtta tcgtgtcttt tctacttatc gtcatggtca
     2341 gctatttatc ggaagctttc tggtagctat gctgtttctg acaggaggac agctcgcagt
     2401 agagcagatt ttcggctgga atacaacagt gagtgtcgtc gttgaatttg tcggaggaat
     2461 ttattttgtc acaaaattga ttcgcgaaag gaagagtatc ctatgattga tttacaaaag
     2521 gtcaataaac tgtaccatgg aaaatcagtg gttaaagatg tacagttaac gattcctgaa
     2581 aagaagttga ctgcttttat cggtccaaat ggtgctggaa agtcaacggt gttgtcgatg
     2641 atcagtcgtt tgataccaaa agatacaggt gaaatctatt tagaccataa cgaagtaaag
     2701 gcttggaaat ccaatgaatt agccaaaaaa ttatcgatcc tacgacaatc gaatgaaata
     2761 aatgtccaaa tcaccgtgaa agaattagta gaatttggac gatttcctta tacgaaagga
     2821 aaattgacga caaaagacga ggaaatcgtt caggaatcac tggaacacct tgggttagaa
     2881 gaaatagcgg atcaatcgat tgacactctt tcaggcggtc aactccaacg tgcgtatatt
     2941 gcaatggtgc tagcgcaaga cacggattat attttactgg atgaaccttt gaacaatcta
     3001 gatatgaatt ttgcggttca aatcatgcag atcttaaaaa aattagtaca agaaatcggg
     3061 aaaacaattg ttattgttct tcatgatatc aactttgctg caagttatgc agacgaaatt
     3121 attgcaatga aagatgggca gttattcacg caaggaacga cccaagaaat gataaaaaaa
     3181 gatgtactgg acgaattgta cgaaatggac atacgaattt gtgagattga aggtcgcaga
     3241 ttctgtcttt atttctaaaa ctaacattag gagagaagag atgaaaaaat tagtagtagg
     3301 tatcttgatt ctagcatcat ttggtttagc agcatgtgga aatagtgaca acaccagtca
     3361 ggcagataca aagaaaagca gtacgcaagc tgaaacggca ttaacgataa aagacagtaa
     3421 tggcgatcaa atcgaggtgc caaagaatcc tgaaaaagtc gtcgtattcg acaatggttc
     3481 tttagatacg atggatgctc ttggtgttgg agataaggta gttggagcag ctacaagcag
     3541 ccttcctgag tatctttcat cctataaaaa agtggaatca gcaggtggga tcaaagaacc
     3601 agacttagaa aaaatcaatc agcttcagcc agacctgatc atcatttctg gccggcaaag
     3661 agattttcaa aaagatctaa gtcaaattgc tccgaccatt ttcctttctt tagatgcgaa
     3721 aaatccatgg gaatctttcc agcaaaatgt cactgcttta ggggagatct ttggtaagca
     3781 agaagaagct aaaactcaat tagaggaatt atctagtgca atcgatcaga cgaaaaagaa
     3841 agcagaagct actgacaaaa aagcactagt cacacttgtg aatgaagggc aattatcagc
     3901 ctacggatct ggttctagat tcggtttcat ccatgattta tttggctttg aacaagcaga
     3961 tgatcaaatc gaagcatcca ctcacggtca aagtgtttct tatgaatatg tgctggaaaa
     4021 aaatccggat atcttatttg tagtagacag aacaaaagct attggtggag acgacagcaa
     4081 agatgatatt tctgccaatg aactggtagc acaaacgaat gctggtaaaa atcagcagat
     4141 catctccctt gaaccagatg tctggtattt aagcgggggc ggtttagaat caatgaaact
     4201 gatgatcgaa gatgtgaatc aagcatttaa atagcataaa taacagagga ttaaactctc
     4261 atactatgta acaataatgt ttcttacatt cttaaatagc aagagcctat cctcaaagaa
     4321 gttatcaaaa accatccgta aaatcgtatt gatgatttta cggatggttt ttatttattt
     4381 gggaaatctt ttttcaacgt gaattcattc tctacatcat acgggttgat tctagaatgg
     4441 ggagtggcaa aaaatagtgt gatccatttt ttcagcatca agatcgcctc ctttgatttg
     4501 aatcaaaggt acaaagcgaa acttagataa aacttaaatt tagatatttt tattttgtac
     4561 cgatctcttc tttaaacgat gttccatctg ctgcttctcg gcaataagtc cactagacta
     4621 gaaaatatga gaagctattt tctgtccagc gttcttatta ctcaaagcaa atcgcagttg
     4681 tcacaatctc ttctaaacga tgttccatct gctgcttctc ggcaataagt ccactagact
     4741 agaaaatatg agaagctatt ttctgtccag cgttcttatt acttaaagca aatcgtagtt
     4801 gtcacaatct cttctaaacg atgttccatc agctgacctt cggtattagc tcaaagcact
     4861 gatttcacaa cctctattta ggcatgtgtc tcagcaagcg gttgatagtt gatgatctgc
     4921 atttttcgat aaaaatcggc gggaagctta ctccaccgtt ttaataattg atagaaataa
     4981 ctctcttctg ctttgatttt atactcgctt aatatcaatc caaaactatt cgtttttttt
     5041 gaagctaatt ttttataaat atccaatgct ttggttttat cttcattgac aaagagtgtt
     5101 tttacttcaa tataagaacc ctcgatattt tgtgtttctt cgataaaata gatgtttttt
     5161 atatattcct tcatagtgat tcctccaaag aagtcttttt atttgataag tttactctac
     5221 cgaagagttg ttaataatcc gtgaacaaaa agtaattctt tttccaatag ttgttaaagg
     5281 ttttttattt tttatagtat tttttgttat ttaagaaagg aataaggtta aacaaataac
     5341 aggttgcttt ttgggtcttt ttccgttcaa gaaactgaac gaagaaaagg gaaagttgtc
     5401 tcttttgtga taagagaaat cataataaac gaatgactta ctttccaata aattgagaac
     5461 agtcagaacc agacaaatta gttgcatctg atgaataaag tgattattct atgacatgag
     5521 gtgatacaga tgaaattaga agtgacaaaa gaagcacaag aagtattgaa gaataaatta
     5581 gaagcagacg atcaactttt attagatatc gaaaacggtg acggtccatt tgcagaaagc
     5641 caagtcagtt gccaattgga cacggcattt cgattgatca ttgtcaaaaa agatacacaa
     5701 acagatctaa gtctttattc tgtagtggct gatacaccag ttggaccaat caaaatcaaa
     5761 gacagtgcaa tactttacat ggatgatcca tccacacttg ctttagaacc aacttataat
     5821 agtttacaat taaaaggacc aagcggcttg agaaaaggca acttgcaagt tgttcgaaaa
     5881 gactagaggg ataaaatggg aaaattggcg atcatcagcg acctacatgt ggacatcaag
     5941 aaattcggcg aatcagagct gatgcagctt tggcaagttt tgcaaaagca caagatcact
     6001 cggcttcatc tagccggaga tacggctaat aaagtagacc gttgcctagc tgttgtcgaa
     6061 tttttcgtca ataaaggatt atctacgaca tttcatttcg gcaatcacga gttggcagat
     6121 gtgcccaatg aacagatgat ggaccatttt ccagacccac attttttaaa tgaacggtat
     6181 attgctttaa atgagcagac cgttctttta gggatgaatg gctggtacga ctatcagttt
     6241 tccgaattga aagattacga ggcaatcgta cgaatgaagc gtttgtattg gtatgatcgg
     6301 ttcatccagc gtgggaaaac agatctcgag gtcaatgcag aaatgcttgc agctgcaaaa
     6361 aatttgctag accagctgga agcaaaaaaa cttgaggtga ttttatccac tcatttcgtt
     6421 cctaaacggg catttatcat gtatcaatct gctccttatg agcgctggaa caaattaaat
     6481 gcatttttag gatcagaatc attcggagaa ttacttgacc gttattcaaa tatcaaacaa
     6541 gtagtttttg gccatacgca tcgtcgcttt gaagataaat ccatcggtca cacgatttac
     6601 agctgcagac cttttggtta ttattatgaa tggcagctga cccgagattt tgtcttgaaa
     6661 gaacagctgt tagatagcta tgaacccatg aaattacgtg tcctcttgcg tgcccatcat
     6721 ccggcctttc aagcctatca aacacaacat ttgcaagagg aatttgaaca ggcgatgaca
     6781 atcgttccgt attgagacag cattagtaat gtgtaaaaga aaataccttg taaaacataa
     6841 ggataagtaa gagcagtaga tccatttatc gtggatatgc tgcttttttt gtggaaaata
     6901 atcagcgaat gtttatttga tcggatgatt tgtaactcgc agagtactag atgcaagcgt
     6961 ttattctttt tttgaacgat aatcacaaaa aaaaatataa tatacatttt ttatttaatc
     7021 ttaattgtca aattaagtta gacagatcat caatacttac gtaactcaaa aatacttcca
     7081 aaggtgttcg ataatccagt gattttctag gaatattatt tcgtttagat gcgacagatt
     7141 gaataaaaaa ttcatctact gaattgaaat ccatagattt caataaacca tctctacgta
     7201 acaatccgtt agaattctcg tttaggcctc tttgagacgg cgttcctgga tcagcgaaat
     7261 aaatggcaat atcattgaca ttactgatct gtttccaatt tgaaaattcc tttccacaat
     7321 caaaagtgat ggatttgaat aggtttttcg gtacagattc aaaccattga tttaattttt
     7381 tttcaatatc aatcg
//
LOCUS       DAFEPQ010000095         7343 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.098, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000095
VERSION     DAFEPQ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.098"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            68..151
                     /locus_tag="LP543_002468"
     CDS             68..151
                     /locus_tag="LP543_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBL2322600.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            276..1013
                     /gene="erm(B)"
                     /locus_tag="LP543_002469"
     CDS             276..1013
                     /gene="erm(B)"
                     /locus_tag="LP543_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBL2322601.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1183..1443
                     /locus_tag="LP543_002470"
                     /pseudo
     CDS             1183..1443
                     /locus_tag="LP543_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1606..2271
                     /locus_tag="LP543_002471"
                     /pseudo
     CDS             1606..2271
                     /locus_tag="LP543_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2307..2552
                     /locus_tag="LP543_002472"
     CDS             2307..2552
                     /locus_tag="LP543_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL2322602.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2655..3524
                     /locus_tag="LP543_002473"
     CDS             2655..3524
                     /locus_tag="LP543_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBL2322603.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3505..4239
                     /locus_tag="LP543_002474"
     CDS             3505..4239
                     /locus_tag="LP543_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL2322604.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4272..5184
                     /gene="ant(6)"
                     /locus_tag="LP543_002475"
                     /pseudo
     CDS             4272..5184
                     /gene="ant(6)"
                     /locus_tag="LP543_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5181..5723
                     /gene="sat4"
                     /locus_tag="LP543_002476"
     CDS             5181..5723
                     /gene="sat4"
                     /locus_tag="LP543_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HBL2322605.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5816..6610
                     /locus_tag="LP543_002477"
                     /note="aph(3')-IIIa"
     CDS             5816..6610
                     /locus_tag="LP543_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBL2322606.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            6886..>7343
                     /locus_tag="LP543_002478"
     CDS             6886..>7343
                     /locus_tag="LP543_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBL2322607.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRV"
BASE COUNT         2569 a         1109 c         1542 g         2123 t
ORIGIN      
        1 tgtgtataat aggaattgaa gttaaattag atgctaaaaa tttgtaatta agaaggaggg
       61 attcgtcatg ttggtattcc aaatgcgtaa tgtagataaa acatctactg ttttgaaaca
      121 gactaaaaac agtgattacg cagataaata aatacgttag attaattcct accagtgact
      181 aatcttatga ctttttaaac agataactaa aattacaaac aaatcgttta acttctgtat
      241 ttatttacag atgtaataac ttcaggagtg attacatgaa caaaaatata aaatattctc
      301 aaaacttttt aacgagtgaa aaagtactca accaaataat aaaacaattg aatttaaaag
      361 aaaccgatac cgtttacgaa attggaacag gtaaagggca tttaacgacg aaactggcta
      421 aaataagtaa acaggtaacg tctattgaat tagacagtca tctattcaac ttatcgtcag
      481 aaaaattaaa actgaacatt cgtgtcactt taattcacca agatattcta cagtttcaat
      541 tccctaacaa acagaggtat aaaattgttg ggagtattcc ttaccattta agcacacaaa
      601 ttattaaaaa agtggttttt gaaagccatg cgtctgacat ctatctgatt gttgaagaag
      661 gattctacaa gcgtaccttg gatattcacc gaacactagg gttgctcttg cacactcaag
      721 tctcgattca gcaattgctt aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa
      781 acagtgtctt aataaaactt acccgccata ccacagatgt tccagataaa tattggaagc
      841 tatatacgta ctttgtttca aaatgggtca atcgagaata tcgtcaactg tttactaaaa
      901 atcagtttca tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc gttacttatg
      961 agcaagtatt gtctattttt aatagttatc tattatttaa cgggaggaaa taattctatg
     1021 agtcgctttt ttaaatttgg aaagttacac gttactaaag ggaatggaga taaattatta
     1081 gatatactac tgacagcttc caagaagcta aagaggtccc tagcgcctac ggggaatttg
     1141 tatcgataag gggtacaaat tcccactaag cgctcgggac cccttgtagg aaaatgtcct
     1201 aagtgtggca acaatattgt attaaaaaaa tcgttttatg gttgttcaaa ttatcctgaa
     1261 tgtaagttta ctttagctga acattttaga aagaaaaaac taaccaaaac gaatgtaaaa
     1321 gaattactgg agggaaaaga aaccctggta aaaggaatca aaaacaaaga gaaaaagccc
     1381 tacaatgccg ttgtaaaaat tggggaaaag ggatatattg attttatctc tttttcaaaa
     1441 taaacatggg gctgacccaa aagtagctcc tatatgaaaa atagaggtct ttccatttag
     1501 atagtagtgg aaagacctct atttatcttt tgaaagtgaa tacaaaaata aagcacactc
     1561 tgctataata taggtgatta atccatttga gagaggtgct ttatcatgta tcaaaattat
     1621 accataaacc aactttgttt gcctatagat cttgaaatta aattagaaga aaatgatttt
     1681 gctcatgcca ttgtgcagtt tgttgactct attcccgatg aagtctttct tccttattat
     1741 caatctatgg gtagacccca atatcatcct cgtatgctac tttccattat tctttgtgcc
     1801 tatattcaag gggtttattc gggccgccaa atacaaaata tgctgataga tagtattcgg
     1861 atgcgctact tatctcaaga gcaattccct aactttcgta ctattaatcg ttttcgcgtt
     1921 catccgatta tgaacgatat tctagatcat tcatttgttc aatttcgtga gctactggtt
     1981 caaagtggct taatcagtgg cagtgctcta tacattgatg gtacaaaaat tgaagcggat
     2041 gcgaataaat actctttcgt ttggaaaaaa agtattttaa agtataagga tagccttgat
     2101 caaaaagccc ttgaaaacta ccaacaaatg gtggaaacaa aaatcttacc tgagttaata
     2161 gatgatttaa aggatgaact ttctatagaa gagattgaaa ggattcgtca atctttggaa
     2221 gaaaaggaaa atcaattaat tgaagcgatt gaccaaaccg aaacagtaga agggggctga
     2281 cccattatta ataaggagtg gacaacatga ttgatgtaaa tgagctattg gaagaagcta
     2341 ttagagaaac tgaaaatcta aatgatggcg aagtttttct tgttaaagat ttattcaagg
     2401 gatatgtggg gaataggata cccagaaagg atcgacttct gcttggaacg ttatttttaa
     2461 accatgttag caaaatgaat ggtaatttaa aggctattga aaagacttca tctaatcagc
     2521 agaggtataa aaagacaatt gataagtttt aactatcttt tgtgaaatta aaaaaattat
     2581 aaatatgttg agtagaagca aaagatttgg cttggaattt ttacagcagt gaatcgataa
     2641 aaggagcatg cttgatggta gataatatta ttaaatcagt agcagagaaa ttatcctctc
     2701 tgtcttatat agaaggtatt gttttaggtg gttcacgtgc aaggggcacc catacagagg
     2761 attcggatat agatatcggc atctattaca attcagaatc atttgacata aatactatta
     2821 atcaattcgc tacaaagctg gatgatgagc atagaaataa ccttgttgta cctcccggag
     2881 catggggtga ttggattaat ggcggcggat ggttagtcat aaacgggtat catgtggatt
     2941 taattttacg tgatattaaa cgtgtggaac aaataatgaa agatacagag cacggaattg
     3001 ttactgccaa ttatcagact gggcatcccc atggttatat tagtgcaatg tatcgaggag
     3061 aattagcgat tagcaaaata ctatatgcta agaatgaaag cttatgcgaa ttaaaaaaac
     3121 aggcagaaac ttatcccaat gctttgcaga aaagtttagt taactttttt atgtttgaag
     3181 cagggttctc tttaatgttt gtaaaagcaa attcgggaac agacgataaa tattatattg
     3241 cgggtcatgt ttttcgtata gtttcatgtt taaatcaagt gttatttgca tgtaataatg
     3301 cttattgtat caacgaaaag aaagctataa aactgcttga aacttttgaa cataaacctg
     3361 aaaaatatac cgagaaggta aatcatattt ttgaagtact cggtatctca ctttttgaat
     3421 gctacgacat gaccgagaag ctttataatg aagtgaatga aattgtatcg gagataaata
     3481 actttttaaa cgaggagagt tcagatgaaa gaaaacaaat atgatgataa tatatttttt
     3541 caaaaataca gtcaaatgag tcgctcgcag aaaggactgg ctggtgcggg agaatgggag
     3601 actttgaaaa agatgctacc tgattttaag ggtaagcgtg tgcttgattt aggatgcggc
     3661 tatggatggc actgtatata tgcgatggaa aacggtgctt cctctgtagt aggtgttgat
     3721 atttctcata aaatgctcga agtagcaaaa ggaaaaaccc attttccaca gattgaatat
     3781 gaatgctgtg ccatagaaga tgtggatttc ccagaggaga gctttgatgt aatactaagt
     3841 tcgcttgcgt ttcattatgt agcagactat gagaatttaa taaaaaagat atataggatg
     3901 ctgaaggctg gtggcaattt agtttttaca gttgaacatc ctgtttttac tgctcatgga
     3961 acacaagact ggtattataa cgaaaaagga gaaatactgc atttcccggt ggacaattat
     4021 tattatgagg gcaaacggac agctatgttt ttggaagaaa aggttacaaa atatcataga
     4081 acactgacca catatctaaa tacactgctt tcaaatagtt ttataataaa tcagattgtg
     4141 gagccacagc cgccagagaa catgatggat attccgggga tggcggatga aatgcgacgc
     4201 ccaatgatgc tgattgtatc ggcaaaaaag aagatgtaat aatatagaaa aaataaacga
     4261 ggagtatgta aatgagatca gaaaaagaaa tgatggattt agtactttct ttagcagaac
     4321 aggatgaacg aacgtattcg aattgtgacc cttgaggggt cacgcgcaaa tattaatata
     4381 cctaaagatg aatttcagga ttatgatatt acatattttg taagtgatat agaaccgttt
     4441 atatctaatg atgactggct taatcaattt gggaatataa taatgatgca aaagccggag
     4501 gatatggaat tattcccacc tgaagaaaag ggattttcct atcttatgct atttgatgat
     4561 tacaataaaa ttgatcttac cttattgccc ttggaagagt tagataatta cctaaagggc
     4621 gataaattaa taaaggttct aattgataaa gattgtagaa ttaaaaggga catagttccg
     4681 actgatatag attatcatgt aagaaagcca agcgcaaggg agtatgatga ttgctgcaat
     4741 gaattttgga atgtaacacc ttatgttatt aaaggattgt gccgtaagga aattttattt
     4801 gctattgatc attttaatca gattgttcgc catgagctgc tgagaatgat atcatggaag
     4861 gtcggcatcg aaacaggctt taaattaagt gtaggcaaga actataagtt tattgaaagg
     4921 tatatatccg aggatttgtg ggagaaactt ttgtccacct accggatgga ttcctatgaa
     4981 aacatatggg aagcattatt tctatgccat caattgttca gggcggtatc cggtgaggtg
     5041 gcggaaaggc ttcattatgc ctatccggag tatgatagga atataacaaa atataccagg
     5101 gacatgtata aaaaatacac tggtaaaacc ggctgcctgg atagcacata tgccgctgat
     5161 atagaagaga ggcgggaaca gtgattacag aaatgaaagc agggcacctg aaagatatcg
     5221 ataaacccag cgaaccattt gaggtgatag gtaagattat accgaggtat gaaaacgaga
     5281 attggacctt tacagaatta ctctatgaag cgccatattt aaaaagctac caagacgaag
     5341 aggatgaaga ggatgaggag gcagattgcc ttgaatatat tgacaatact gataagataa
     5401 tatatcttta ctaccaagac gataaatgcg tcggaaaagt taaactgcga aaaaattgga
     5461 accggtacgc ttatatagaa gatatcgccg tatgtaagga tttcaggggg caaggcatag
     5521 gcagcgcgct tatcaatata tctatagaat gggcaaagca taaaaacttg catggactaa
     5581 tgcttgaaac ccaggacaat aaccttatag cttgtaaatt ctatcataat tgtggtttca
     5641 aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg
     5701 ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat
     5761 aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc
     5821 taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga
     5881 tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata
     5941 tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga
     6001 catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca
     6061 tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta
     6121 tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt
     6181 tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga
     6241 attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga
     6301 cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga
     6361 ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa
     6421 agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc
     6481 cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt
     6541 tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga
     6601 attgttttag tacctagatt tagatgtcta aaaagcttta actacaagct ttttagacat
     6661 ctaatctttt ctgaagtaca tccgcaactg tccatactct gatgttttat atcttttcta
     6721 aaagttcgct agatagagtt ctatattaga gatgtaaaga gttttggtga agaggtttgt
     6781 gagtatggct tttataacca gggtgcaatg agaagcacta acagcagcta gcttaagaac
     6841 gcataggata tactttttat ggaagtcaca gaggaggaat aaaatttgaa caggattaac
     6901 agagtaacct ctattcttat tcagctgcaa tcaaaaaaaa taattcctgc caaagaaatt
     6961 gcacagcgat ttaatataag cttaaggaca gtttacagag atatccggac acttgaagaa
     7021 gccggaatac caattggatc tgaggccgga aaaggatatt ttcttgtaga gggcttccta
     7081 cttccgccgg taatgtttac agcggcggaa gtgggtgcat tgattacagc agggaaattt
     7141 ttaaattgcc atggagatga atcatttata aaggattttg attcagctat gtataaaatc
     7201 aaatctattt tgaagcatgg tgaaaaaaac tatgcacagg agttggagaa cagtattaac
     7261 gtgtacagca catctggaca gaaaaataca ttggcggata acgttattgc agcaattcag
     7321 actgcaatct gcaataaaag ggt
//
LOCUS       DAFEPQ010000096         7216 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.099, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000096
VERSION     DAFEPQ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.099"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(48..1514)
                     /locus_tag="LP543_002479"
     CDS             complement(48..1514)
                     /locus_tag="LP543_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBL2322608.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1901..2497
                     /locus_tag="LP543_002480"
     CDS             1901..2497
                     /locus_tag="LP543_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL2322609.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2555..3037)
                     /locus_tag="LP543_002481"
     CDS             complement(2555..3037)
                     /locus_tag="LP543_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322610.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3143..3340)
                     /locus_tag="LP543_002482"
     CDS             complement(3143..3340)
                     /locus_tag="LP543_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322611.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3635..4393
                     /locus_tag="LP543_002483"
     CDS             3635..4393
                     /locus_tag="LP543_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBL2322612.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4453..4881
                     /locus_tag="LP543_002484"
     CDS             4453..4881
                     /locus_tag="LP543_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322613.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4927..4999
                     /locus_tag="LP543_002485"
     tRNA            4927..4999
                     /locus_tag="LP543_002485"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4960..4962,aa:Gln,seq:ctg)
     gene            complement(5149..5520)
                     /locus_tag="LP543_002486"
     CDS             complement(5149..5520)
                     /locus_tag="LP543_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL2322614.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5735..5920
                     /locus_tag="LP543_002487"
     CDS             5735..5920
                     /locus_tag="LP543_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322615.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6003..6191)
                     /locus_tag="LP543_002488"
     CDS             complement(6003..6191)
                     /locus_tag="LP543_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322616.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6215..6478)
                     /locus_tag="LP543_002489"
     CDS             complement(6215..6478)
                     /locus_tag="LP543_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322617.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6636..6893
                     /locus_tag="LP543_002490"
     CDS             6636..6893
                     /locus_tag="LP543_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBL2322618.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6964..7155)
                     /locus_tag="LP543_002491"
     CDS             complement(6964..7155)
                     /locus_tag="LP543_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322619.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
BASE COUNT         2354 a         1398 c         1149 g         2315 t
ORIGIN      
        1 aacaggaact gctgcaaata agcaattcct gtttttatgt tcattgatta atgaatagag
       61 cccattagtt ttttgattgg ttctttcaca gtaaatagca agataccacc gacgattgct
      121 actgcaccga taataccgaa gtaagcagat tctgttcccg gtgtgtagaa gcgtgcaatt
      181 tgtgcattga ttgcttgtga agaagcatca gctaagaacc aaatagcgat tgtttgtgat
      241 tcaaatgctc gtggtgctaa ttttgttgtc acagacaaac caactggtga aagacacaat
      301 tcagcaataa ccacgatgaa gaaacttccc aacaaccaca atggacttac ttttccactt
      361 gtaccataaa gcatacccgg caacatcaat agcaagtacg ataatcctgc aaataaaagt
      421 ccaagagaga atttcactac tgtagatggt tgacgttcac caagtttcgt ccacatcgtt
      481 acgaatactg gtgtcagaat aaagatgaaa attggattca atgattgata ccagcttgct
      541 tggatcgtta caccaaatag cgtagattgt gtacgttcac tagcaaataa agctaagata
      601 gatgaacctt gttcttgtaa tgaccagaaa atgattgctg caaggaataa tgggatataa
      661 gcccatactc ttggtttttc ttcagcagtt acatcttttg aagtcagcat tttgataaag
      721 taatacaacg gtaagataat ccccaataca ctgattgtat tgacaaaaaa gtcaattgtc
      781 agatgaccag ttagttgtgc cccgccgaat actgcagcga taacaacaag tgcgatgatc
      841 aagttacgga tgaatccttt acgttcttca gttgtcaaag gattcggcgg tgttgtaccc
      901 actccgtcta atgtcttgcg tccttggatg atgtattgta ataatccaaa gaacatccca
      961 atagcagcta aagagaaccc taaatgatag ttatattctt gaccgattgt accaacgatt
     1021 agtggtgcta ataatgcccc taagttgaca cccatgtaaa aaatcgaaaa tcctgcatct
     1081 cttcgcaagt cagtctttga ataaagatgt ccgaccatgc cagaaacatt tggtttcaaa
     1141 aaggcggttc cgataataat caatgccatc gaaacaaaca atgtacttac accaaatggt
     1201 aaagccaaga cgatgtggcc aagcataatg aaaactccac cgaagaatac agtgcgtttt
     1261 gctcctaaca ctcggtcaga tacccatccg ccgacgatac tagacatgta gatcaaagat
     1321 ccgtagatcg acatgatagc taatgctgtt gctcgtggta atccaagtcc cccgttggca
     1381 actgtatcgt acatataata gataagtaat gcacgcatac cgtaataact aaatcgttcc
     1441 cacatctcag tgaagaacaa agttgacaat cccttcggct gcccgaagaa gcccttgtct
     1501 tgctgttttt ccataatcta taccccaatt ctttaaattt tcagaaaata ttatacatct
     1561 ttaccgaaaa atttaactaa aaaacttatt taagaaatat aaactaattt tctataaaag
     1621 caagtaattt ttaatggcaa tatcataaaa aaaagaagat agagatttaa taattatata
     1681 tcagctgttt ttttcaaata agcaaaaatt cctaaaacac aacaatgaaa gcgataacta
     1741 agaaaatcct tacataacga tttttcttat taaaattgtt ttttgaactt cgcttatttt
     1801 ttgtgagcat atctgtttac ttactttttg taaggtgcta tacttcattt cgtaatcaat
     1861 taaaaaaatg caaaacgcaa atcaaaggag aaattttatt atgacaactt ttactgacac
     1921 tttgaaaaac cgccgttcaa tctatcatct aggacgtaat gtttcacttt ctaatgaaga
     1981 attaacaact ttgatcaaag aagcaattaa agaaagccca actgctttca atgctcaatc
     2041 aacacgcgca gttatccttt tcggagatgc acatgaaaaa ctttgggaaa tcactgaaga
     2101 agcacttcgc ccattgactc cagcagaagc tttcccaaat acacaaaaca aattagctgg
     2161 tttcaaaaat ggttatggta cagtattatt cttcaaagat actgacgtgg taaaaggtct
     2221 acaagaacaa tttgaattat atgcagacaa cttcccagat tggtcagaac aatcaaacgg
     2281 aatcgcaaca gctaatactt gggtagcatt agttgataaa ggattgggcg caaacttgca
     2341 acattacaac ccagttattg acgaagcagt tgcaaaagaa tggaatatcc cttcaaactg
     2401 gaaactacgt tcgcaattag tattcggttc accagaaaca ccggctgggg aaaaagaata
     2461 catgaacgat gccgatcgct tccgcgtatt tggataattt tatttgaaat aaaaaacaga
     2521 ggttgtgaca aacatcttgt cgcaacctct gtttttattc tggatcaatc ataatatcaa
     2581 tctctgcctc atcgtttaag cgttgggaca aaaatggaac taattcttct aatgatgcag
     2641 aaaatttcag catcggtcct cgccatccgt ttcggaaaaa attcattttt acgattttag
     2701 aaccttttgg ctgatataat acaattttat tgatctcaga aaaaggaaca ccttttttgt
     2761 caaagctatt caaaacaagt ccttcttctg tgattcctct actgtccaac aaaaaactaa
     2821 taaaaatcat tgctgacaag atcccttttg ccaattcatc tgctgtagga tgactgatga
     2881 gtgtatacca aatgactagt gacactaaga agggtatcac caagtagacc catcgatttc
     2941 gcacagtccg ataaataatt tcttttcgaa ccagccaaag aaataaaaac atcacaacaa
     3001 cgaataaaaa caatagaacc gtaaagaatg ttaccatgac aactcgcctc gctttttctt
     3061 ctccagaaaa taggaaaaga atactgattt accttctcaa agaaattgga cttgcagcac
     3121 gccgcacgtc caattgtttt ctttattcaa atgtatcata ttttgcaaca accatgcttg
     3181 ctttttcatc taataattct tggtcctctg cagcacattg ggcaactttg accacggcgc
     3241 agtttgtcat ttttgataca acttcaccaa tgaccgtttt tgtaaaacca attggtttgc
     3301 atgcatattg ttctcctaaa ttaatcattt cttccgtcat caaaatcctc ctaaactgct
     3361 ggaacttttc tccacattac cactgctatt tctttggaga tttaactcat tgatttttat
     3421 ttacagttaa aaatcaacct tacaggcatt atagcgtact gttttcgttt tcacaattaa
     3481 aacagctgtt gtatttagaa gaattttttt caaaaaatta aaacatattc cttaaaaact
     3541 aattatttcc tacatattaa aatataatac ttccatttcc aataaaaata catttataat
     3601 aggaaagata aacagctgaa aagggagatt tcaaatgaag aaaaaaacgt tcaaaaaact
     3661 attgtttcta gggggcacag ctgctctttt attaagcggt acaagttatt acctacatat
     3721 ccattctcca gaacatacaa tgaacactgc acaaaaaagt tcaacacaaa aaacgaaaaa
     3781 gacaaaagag aaagcagaag ttttactaga tgttccatta gagagccagt ttgattctcc
     3841 ttctttagaa aatggctgtg aagtcacttc gctttctatg cttttatctt tttatggtta
     3901 tgaaacaact aaaaaccagt tagctgagca attggattac gtccctgttt tcaatgcaga
     3961 tgggactcat ggcgatccta atgaaggatt tgttggtgat atcagtggcg gagactgggc
     4021 tatggtcgtc tatgttcccc cagttgcctc ccttgctcag caaatcgtcc aaacggatta
     4081 tcacactttt ccaaaaaccg atgctcaact cgatgatatc aaaaaagcac tatctaaagg
     4141 aaatcctgtc tggacttctg tgactatcaa ttttgaagtc ccagatgaaa ctgattttat
     4201 gacttggacc acaaataatg gagaagtaag agtcactcct cttgttcatg cttgtgtagt
     4261 cactggatat gacaaagaaa atatttatgt caacgatccc tatggtgtaa aaaatcgagc
     4321 agtaccgatc aacgattttt cggcgatttt cactgcaatg ggtggacaga tgctaacttt
     4381 ggagaaaagc tagtaattca aacaaaaatc ccttatactc aagataccaa acctttgaag
     4441 ggagcgtaga taatggaaaa attcacacat aaaaaaatgg atccaaatga aattccgatt
     4501 atttttgtcc gtgactgcaa gggaaatgtt caaggtaaag tctctattaa tgagtggaac
     4561 gagcgtcgca gacctgctac cctcaatgaa ctggaaatca aattgtatcg tcaatctctt
     4621 gtttattacg cagatcaaga gtacgagaaa gccaccgatt tattgaaatt tctaattgct
     4681 cgaacagagt atactcgctt tgaatatatt gaacgtttag caaacatcta tcatattatg
     4741 aatgaacctg tcaaagaata tcagttattg gacaccgtct tgtcagtagc agaactgatt
     4801 gcccttcctg ctggattaga aaagaaacta gtacgccgcc ttttacgagt aaagcaacaa
     4861 ctatccgatc aagaaaaata acttgcgttt ttttaggaaa gtgctatgat tattttgttg
     4921 aataaatggg atgtagccaa actggtaagg cacttgactc tgaatcaagc aattgtaggt
     4981 tcgaaccctg ccatcccaat ttgatgtgct tgctgaaaaa atgaaccccc tgtaaaagaa
     5041 caatcttttt gcagggggat tattgtatta tttttactac ttgtatttag tttacataat
     5101 taaattatga ataataaaat cgggcgtatt cttaatacaa aaactgattt acccaataat
     5161 tgctaatgct ttctccggaa ctttttctct aggaacttcc tcatacgttt ctatgtgtgc
     5221 tcctttattg ctaattttca aataatgatt ttcttttaac acagaaagac cattaaattc
     5281 aaccgttcgt tctttcccat tggcatcagc agctttctgc acataccttg ctgtaccata
     5341 ttcatttact tcctctggtt tttgtgtctt tacatagacc tctccttttt caactaatgg
     5401 atttagctga tcaagtatac cagggatttc tccataactt ccttcagtat aatatttcag
     5461 tccaaatagc aaaataccac taatcgccgc cagtccaatc aaatatttaa tcactttcat
     5521 ctttctacct ccttgttata tattcagtat atgggtcagt taaagaaatt ccatccgtcc
     5581 caagttggag atttttctcc tactttagaa gcgagaatcg agtgtttttt tagtaaaaat
     5641 gatcgttatt tgatattatt gacgtgtgga taaatcagtt tttctcgttt tgtccatgta
     5701 aaaaatcatg ttgatagcaa aggatgtgtt caaaatgaat caagaagaat tatttcaaaa
     5761 agtcaaggaa atgattaaga acggcaattt cgatggcgca aaacgattta tcgaagaaca
     5821 taaagaacaa ttaggacctt ataaagagaa agcccaaaat cttttaaaag atgtaaatat
     5881 cgacagtgtc aaaaacaaat ttaaaaatct attcaaataa gtaaaacttt cacttttgta
     5941 aaatcaaagg aggacaatgc tgctgcatcg tcctcctttg gttcttttct gaatacttca
     6001 tactaaaata aacgtgtcgg atcgtaatga taagtcacat tgccgttcag tgaggctttt
     6061 cccaccacat atttacgatt ctccaacttc agccacgctt ctcgaaaagc cattaattct
     6121 tctcttttta catcaatctt ttcgattttt ccatcgatca aatcttgaat aagttgttca
     6181 tatggattca tttttttcct ccatctgctg tcaatcaata agttgtaaaa ataggttcat
     6241 atgctttttt ggtatctaga tcttctctga ctttgactgt cgtataaata attttttcta
     6301 ctgggatact ataataactt ccttcaccat tattgaatcg gtgaacgtct ttctttgcta
     6361 aaataccatt tattttattc gtcagtacac gttgttcgac actgttactt acaacatagt
     6421 aaatcacccg aatattattg atcaaatgca aatcgatttc taacactaca tgatccatga
     6481 aaatcccact ccttctttca ctttgattat agcaagtttc tcctctaatt tcaaaatcga
     6541 tacagatttt accggataaa tccacaaaac tgcttgcgcc atttcgcaca aagtgttatt
     6601 ttgtatcatg gactttattt atcggaggca atagtatgat cgcgatcggt caatttgttt
     6661 tttatattcc tttttttatt atgataagca ttcttttcta ttacataaaa tggacaaaaa
     6721 agaagttttc agttttactg gcctctttac ctgctgtata ttttacttat caaatttttt
     6781 cttttaggca ttgggaaaca acgtctgtat taataacaca catcatcgaa ttgacgcttt
     6841 cagtaatttt tcttattatt tggatctatt ttttatataa gaatcagaat tgaatccctc
     6901 gcatgtttaa aaagaaaacg attaaagatg cagcaaattt ctgccgatct ttaatcgttt
     6961 tttctattct gttataccaa aaaaatgttt gaatcggtga aggatcgttc cttgttttaa
     7021 ttctataaat tcttgtgcga tcgggtactt ttcttccaaa ttttctttgt tcttttcgat
     7081 caggcttttc gcttcttcat attttttatc tgcacatagt gcttttactt cctgtaatac
     7141 ttttcctgct tccatttgcc tcaccctctt gccattttat tgctttaagt tttatttaag
     7201 ttatacgcat tattct
//
LOCUS       DAFEPQ010000097         7187 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.100, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000097
VERSION     DAFEPQ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.100"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="LP543_002492"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="LP543_002492"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBL2322620.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="LP543_002493"
     CDS             complement(1172..1867)
                     /locus_tag="LP543_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBL2322621.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="LP543_002494"
     CDS             complement(1880..2596)
                     /locus_tag="LP543_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBL2322622.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSILYK"
     gene            2926..3534
                     /locus_tag="LP543_002495"
     CDS             2926..3534
                     /locus_tag="LP543_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBL2322623.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="LP543_002496"
     CDS             3859..4182
                     /locus_tag="LP543_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBL2322624.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="LP543_002497"
     CDS             4364..5734
                     /locus_tag="LP543_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBL2322625.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            6081..>7187
                     /locus_tag="LP543_002498"
     CDS             6081..>7187
                     /locus_tag="LP543_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL2322626.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKK"
BASE COUNT         2110 a         1445 c         1343 g         2289 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcgttatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttatata aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaacat actaagatta gaggggaaca tcattgacga atgatgttct cctctatttt
     5881 ctatttttag attatagtga agagtgaaag aaagttcgaa caaagccttg aggactacga
     5941 gtccctatca aaaaccgaac ttctttactg tcattttaga atcaggttta cgtatgttgg
     6001 ggccatgagc ccgtgtatag gaaattggaa tcgaaatgaa gcagtaaagt tctttcaaaa
     6061 cttaaaaagg aggaaaacaa atgccttaca ttcttgcatt agatgtttca atgggacaca
     6121 gctacagtgt cctatacaaa gacgacacct gcttatttga agacgagatt gaacacactc
     6181 aaagaggatt tcatgcatta ctcgaagaga tccagcactt gcctgagcgt ccattgattg
     6241 tttttgaagc aacggggatt tattccagac cagtcgaaaa gttctttcaa gatcatcact
     6301 tttcttactg tttgttaaac ccgttagagg cgaaaaaaca gatggaagaa agcaccttac
     6361 gcagttggaa aacagataaa tcggatgccc accggctcgc acagactcat cttaaaaatc
     6421 aacgtcagcc aaaagaagta caaagcaatt tttacttaga gatgcgcgat ttagcccgtt
     6481 tttatcaaga aattgagaag aaaatcactc gtctgcgcat ggatttacac aactgccttc
     6541 aattgacttt tccagagctt gaacagttct tttcaaacag gctgacccct tacgcattga
     6601 cattgatccg tctttttccc catccagatt ttgtattggc atctactcgg acaaaaataa
     6661 aaaataaact gatcaacgaa acacggaaaa aaatctccgc gaatcgtgcc gagcaaaagg
     6721 cagatcaaat catccactat gcacagtgtg cctatccagc tgttgaaaaa gacagtatcc
     6781 actgtcaaaa aacgatctat tacgccgaac ttctccaaga tttactcgaa caaaaaaaag
     6841 cactggcgac acaaatgatc aaaaaagccg aaggctctcc ttgctttttg ttgtaccaaa
     6901 cgttccctgg gattggcgca ctcacagccg ctttattgct cggtgaatta ggagatatca
     6961 cacgatttaa aacccataaa caattgaatg cctttgtcgg aatcgacatc cgacggtatc
     7021 attcaggtaa gtatactggc caagaccgca tcaataagcg tggtaatccc aaagcacgga
     7081 aaattatttt cttcaccatt cgaaatatga ttcgccagca acgcgcagct cctaatcata
     7141 ttgtcgatta ttactacaaa ttaaaaaagc aacctatccc taagaaa
//
LOCUS       DAFEPQ010000098         6905 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.101, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000098
VERSION     DAFEPQ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.101"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(26..319)
                     /locus_tag="LP543_002499"
     CDS             complement(26..319)
                     /locus_tag="LP543_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBL2322627.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(347..1012)
                     /locus_tag="LP543_002500"
     CDS             complement(347..1012)
                     /locus_tag="LP543_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBL2322628.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1152..1784)
                     /locus_tag="LP543_002501"
     CDS             complement(1152..1784)
                     /locus_tag="LP543_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2322629.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1791..2858)
                     /locus_tag="LP543_002502"
     CDS             complement(1791..2858)
                     /locus_tag="LP543_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL2322630.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2855..3586)
                     /locus_tag="LP543_002503"
     CDS             complement(2855..3586)
                     /locus_tag="LP543_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBL2322631.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(3754..4233)
                     /gene="greA"
                     /locus_tag="LP543_002504"
     CDS             complement(3754..4233)
                     /gene="greA"
                     /locus_tag="LP543_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBL2322632.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4369..5544)
                     /gene="mltG"
                     /locus_tag="LP543_002505"
     CDS             complement(4369..5544)
                     /gene="mltG"
                     /locus_tag="LP543_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBL2322633.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            5709..6869
                     /locus_tag="LP543_002506"
     CDS             5709..6869
                     /locus_tag="LP543_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBL2322634.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
BASE COUNT         2029 a         1465 c         1080 g         2331 t
ORIGIN      
        1 aaaacttgtg tcaccctctc tttttttatt gttttttaaa gtgataggtt ggctcctcca
       61 ggttgggatc ataatcaaca agtaaatcat atcctttaaa gaaccattca tctgcttcct
      121 cgataaaaaa aagtgtccca tccatttcca ctttctttac aggatgttct ggctgttcaa
      181 ctgacattcc aatcgaaaaa ccatcatgaa cctctgtttt gccgtagact tttccaaaaa
      241 aacgtattcc gtaagtttct ggcagatcaa tttctgagtc aaaccacttt tgtgcttttg
      301 gcgttaccgt taattccatc ccaacaacct actttctctt ttctttctat tgattcatct
      361 tatcgtctag aatatccaca tcgcctgtct ctccaatcac cagtagatta tcattttcgc
      421 gaacatattc atctgctgca ggagaagcga taaccgtttg atctactctt cgtatggcga
      481 ctactgttaa gtcaaaacgt tgcctgaaat ttaattcagc taatgttttg ttgaaaaatt
      541 taggattgga gactcggatt tctgctaaag aatatttatc tgacagctct aagtagtcta
      601 gaatattctt tgatactaag ttatgtgcaa tccgtatccc catgtctcgc tcagggtgta
      661 cgacacgatc tgctccgatc ttctctagta cacgggcatg gtactcattt tgggcttttg
      721 ctaaaatatt cggtacgccc atttccttta ccatcagagt aactaagata ctggcttgga
      781 tatcttctcc aatagcgaca attacatgat cgaaatttct tatacctaat gaacgaagtg
      841 tcatctcatc ctgtgcattt gccacaacag catgagtggc tatattcata tattcattga
      901 ctcgatcttc attactgtca atagccagta cttcttgtcc agcttcgatc agtgtacggc
      961 agatgcttcc gccaaatcgt ccaaggccta caattgcaaa attttgtttc accattttcc
     1021 tcctttagta tcttcttttc tcagtataac aaagcctttc caaaaacaaa agcaagcatc
     1081 attattgtaa aaaagccaag aaaaataata gttattttct cagcttttga ttatcctctt
     1141 tatcatcgtt tttatttcac caaaccatgt ttaaacgcat agatagctgc ttgagttcgg
     1201 tcttctacct ctaacttagc taatatattc gacacatgcg ttttaactgt tttcagtgtg
     1261 ataaacaatt catccgcgat ttcctgatta cttttccctt cagaaatcag catcaaaatt
     1321 tcattttctc gattcgttaa ttcttcatgc aatactggat catttcgccg actcattttt
     1381 tccatcattt ttgttgtcac ttcaggttcc aatactcgct cgccgcgttg cgtagccctt
     1441 atagcatcag ctatttcatg tgcggtagac gtcttcaaca aatatcctgc tgctcctgct
     1501 tcaattgcag gataaacttt ttcatcatca ataaaacttg tgacgataat gattttggct
     1561 tttggacaat cttttaagat tgcttttgtt gattcgatgc catccatttc ttccatgact
     1621 agatccatta aaatgacatc tggtcgaagt gccatcgcct tttcatagcc ttgacgtcca
     1681 ttttcagctt ctccaatcac ttcaatgtct tcttggatag ataaataaga agagacgcct
     1741 aagcgtacca tttcatggtc atctactaat aaaactttta tcatctttat ctatgcctcc
     1801 ttcatcaaag ggaccttgat ctccacgctc gtaccttgcc ctttaaaact aatgattttt
     1861 actgttccac caataccagc tactcgttct ttgatattgt tcaatccata acttcccgtt
     1921 ttggtttcat tcatattaaa tcctgtcccg tcgtcaatga tacgtaataa aagattgtta
     1981 tctattttat gcaaatagac ttctaattca ttagccttag catgtcttaa tgtgtttgat
     2041 aacaattctt gaacgattcg gaacaagtga tcctcaatgg aactagttaa ttttacatct
     2101 tctacatccc attttagcga aatctggatt ttattctgca attccttcaa taactgctca
     2161 atgccttgtt ttaaactttt ttcttctaaa ttgactggac gcagatgaag aagcagcgca
     2221 cgcatctcag actgggaagc gttgatgatc tctgccacca ttttcaactg cttacggaac
     2281 atttctggtg tctcactttt ttctgcttgc tccgtcaatg ccgacatcat catcatcgct
     2341 gcaaataatt gctgtgaaac tgaatcatga agctcacgag ctaatcgatg cctttctaat
     2401 tctaaaatct cttctttggt ttgtccatct acatattgag gacggctagt cataatctgt
     2461 aattcactgg atacttcgat cattttttct tttattttag caatttccat atcaatatct
     2521 ttgatgtaca gatcatccga cgcgcttggg atcggctgat gcaaaagttt ggattcataa
     2581 ttgccagaag aaagtgcacg aagcttttct tcgattttcc cgtattttgc cttttgaata
     2641 agtgataaca atagaaatac gatcaatcct acacctaatg aaattgccat caaatggaag
     2701 ataagcggaa cgtataataa acgcgcacgc agcaattctc cccaccaatg actttggtta
     2761 ctggcataaa aataagtgaa aagtgaaaat agaataataa ggaaagccgc aatccccgaa
     2821 taaacagcta gcattgcttt ggatattctt cccatcatat tcgtatcacc tcgacatctc
     2881 caaccaaggt attggtaatg atcttcaatc tgcgaggatt ttcatcataa tcactgctgc
     2941 agattttgat ctgttcgttt tttaaggaaa actgctcttc ttcaaacaag acatttccta
     3001 ctaatgtcgc atgctctaaa cgaatagcca ctcccagggg tactaagatc cgtgttctgc
     3061 ctatcccttt tcttacaatc acaatattat catcttttgg caaaagtgta ttacccaaat
     3121 cgatgatagt atcaccagaa atgatggcaa tgttgatatc atcccattca tagacgtcgt
     3181 ttccaatacg ttggttacca aataattgct gtcttttacg ctcattatta tgtgttttta
     3241 attgctctgt ttggaccatc atgatctgtt tttttcgcca aaaagcattt tttgtcaaat
     3301 ctattcctga aatctctaca cctttcagcc cgataaataa aacggccaat acaaccatca
     3361 tccataaagc aggactgttg actaagctaa aaaaaatgac caagctccct aagatcaatt
     3421 gaaaattttg aaatttagta cgaggatatt tgcgcatagc tagatatata ttgaaaatac
     3481 cgaatattac taaaagcaaa agtccagtat tgtttacgat ttgccaaaca gcaaataaca
     3541 gcagcagtgc ttcgactacc acaaaaaaac gccaagaact attcatcgct ttttctccct
     3601 cctgacacat tctcatttca tcatacctat ttttttgact gttggctata ggactttaga
     3661 tgtaagatcg ttcagtgaaa cctgcaatac tcaaagagaa aagatctaga gtgcgtcatc
     3721 agtaagatga actctctaga tctttcagtc agactacgct tgttctactt tgacgatttt
     3781 aactaacata tcgccacctg gtgtagcaat ggttacttca tcatttttac gtttgccaat
     3841 caatgcttgc gcgataggag aatcattgga aatctttcca gaaaacgggt ctgcttcagc
     3901 acttccgaca atcgtgtact cttcttcatc tccatcaggc aactcgatga acgtaactgt
     3961 tttaccgatc gatacctcat ctgcatctac gccgtcatta tcgatgattt gtgcaaaacg
     4021 gatcatattt tcaattgttg tgattcttcc ttcaacaaat gcttgttcat ctttagctga
     4081 ttcgtattcg gagttttcgg aaagatctcc gaaaccgcga gcgattttga ttcgttcaat
     4141 gatttctccg cgtttaactg tttttagttc ttctaattct ttttctaatt tttctttacc
     4201 ttctagagtc attggataaa ctttctctac catgtgtaac gtctcctttt ttcgataaaa
     4261 taccgcaaaa ggtaatcttt tgaggaatat agtccaaaaa agattaccac gcttttctta
     4321 aaatgtaaat gtttttctaa aaatgatcaa tgactagccc ctgtttttct aagaattatt
     4381 tacatatttt tgcactagtt ccatatgttc ttcatacgtt ttagcgaaat aaacatttcc
     4441 agtgctcgta tctgctacga aataataata gtcattttct gctggttcta atactgcctt
     4501 gattgccttt tcgcttggat tgtcaaaagg accaggccca tagccggtat tggtatacaa
     4561 attgtatggt gaatctacag ctgtatcttc atacgtcacc aattctttat gttctcctaa
     4621 tgcatataaa acagagatat ctgactgaag tggcatattt gccttgattc ggttaaagaa
     4681 gacttgtgca atatttttac gatcattttc ttttacgcct tctttttcaa ctagagaagc
     4741 caaagttaaa acttcttgta cagtcaaatt tttctgtttg atagctgaat aatagttttg
     4801 catgaccgta tttgtcttgt tcaccatctc aatcacaaga tccttaagcg aagtactcgt
     4861 gtagtaatca taagtagctg ggaataagta gccttccaaa cggtattttg tgttctcagc
     4921 tttagaggca ctttccaaca attctgggaa tttttgatac aactcgttaa agaaagtctc
     4981 atcatttact aaatctaaaa attctttctt gtctttacct gttaccttcg ctacccgctc
     5041 agcaatctga tcgatgtcgt agccttctgg aatggcaatt ttcccatctg caacttttgt
     5101 tggctcagat gtgccgcctt cttgcaactg cttgccaatc tcatcaagag tcatattagg
     5161 cgctaactga taataaccag cttgaaagcc agttaagttt ttaaattttg tataataatt
     5221 gaaaacaatc ccacttttaa taatattgtc tttctccaaa atttcgccaa tctgcttatt
     5281 agaagagccg tttgggattt ccacttggac taattggtcg tccgatttat ctaaaggctt
     5341 caacccgcta tctacataac gatatacggt aaacccgaga aatccgccaa taatcaataa
     5401 tgtaagagcg atcactaaaa tgatcttcct cacgatctta tcttcttttt tacgcagatt
     5461 tctccgcttg tcgattatat ctgctgtagc tgccttactg gcttcttttt tgtctttgct
     5521 tgggtgttgt ttgtttcctg acaaaacaac tcctccttac tatcatcttc gtaactctgt
     5581 ttattataca tgaaatttct actgccgtga attattttac caaatattat gtcaaaattg
     5641 taagtggcaa atgctctttt tgaggattac aatgaaaaat agttaaaaat ggatagaaag
     5701 gatttaatat ggcaaaagga aaacatcaaa aaaaacagca gatctctctt tcttttctcg
     5761 cagttttgtt gctggctgtc agcttctttt caacaggcat cattacagga tggcaattaa
     5821 caaaacaacc tcaaccacaa atttataaaa cggaacccgc acaacttgct tccaatttaa
     5881 catctaatga gcagatgctt gccagcaaaa ttgatcaatt actgcaaaaa tcagattata
     5941 tcggttcgat ttatgtacgc aaaaataatc gagtgatatt agaaaaagga tatgggtacg
     6001 caaatctacg cataaaagaa tcgaattctc cttctcttta ctatcaaatc ggttcgatcc
     6061 aaaaagcgat gactgcttta cttatcctaa aacaaattca atctggcaag ataacttttg
     6121 acacaaagct atctgaattt tatcctcaga ttcctggaag tcagcaaatc tcaatcaaaa
     6181 atatgcttta catgcgctct ggtcttcatc gaaccgcttc tccaaaaaaa cctatgtcag
     6241 atagtgagat cattcagttt gcccttcatc atttgaaatt tactggttat gatacgtatc
     6301 attatgaacc attaaattat acattgctta caggggtatt gatcaaacta atgaatcagc
     6361 cttatgaaac tcttttaaaa aaagaaatca ttcggccact ccatctagaa catacagatt
     6421 tttatgaaaa tgtaaaaaat tcccctcagc atgctgtatc ttaccaaatg tctgcaactg
     6481 acgattattc aaaagcattg gtcgagcctg aaacagacat ccgaaatgaa ttaggcacag
     6541 gaaatataag tatgtctgtt tatgacttga acaaattctt taccagtgtt ctgtctggcg
     6601 atattatacc aaaagaacta cttttctcat tgtggcaaaa taatggtgat aaacatcctt
     6661 acagcggcgg tgtttacagt ggaaatgatt atatccttgc tcagggaaat atcaatcgat
     6721 tccatgcagt agcggcgatg aaaaaagaca caaaagatgc aatcgtcatg gaaagtaata
     6781 tccaatctga taaaaacatc aaattgccgg ccactgattt aagaaatcag atttacgaac
     6841 tgatggaagg tgttaattta aaaagctaag tcgagacaaa aaaaccagca ggatgctggt
     6901 ttttt
//
LOCUS       DAFEPQ010000099         6728 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.103, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000099
VERSION     DAFEPQ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.103"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..437
                     /locus_tag="LP543_002507"
     CDS             <1..437
                     /locus_tag="LP543_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL2322635.1"
                     /translation="TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIES
                     EGTVDSITTNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYN
                     RKDNQVTVSLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            682..798
                     /locus_tag="LP543_002508"
     CDS             682..798
                     /locus_tag="LP543_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBL2322636.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            814..2733
                     /gene="tet(M)"
                     /locus_tag="LP543_002509"
     CDS             814..2733
                     /gene="tet(M)"
                     /locus_tag="LP543_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBL2322637.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2831..4204
                     /gene="tet(L)"
                     /locus_tag="LP543_002510"
     CDS             2831..4204
                     /gene="tet(L)"
                     /locus_tag="LP543_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBL2322638.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            4768..6030
                     /locus_tag="LP543_002511"
     CDS             4768..6030
                     /locus_tag="LP543_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBL2322639.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            6275..>6728
                     /locus_tag="LP543_002512"
     CDS             6275..>6728
                     /locus_tag="LP543_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBL2322640.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKI"
BASE COUNT         2294 a          985 c         1381 g         2068 t
ORIGIN      
        1 atacaaagac cgtccactct gcttatatag tgagtgtcta tgtagacagt actggaaata
       61 tggtcctcat taagaatccg accattacta gcataccaaa gaaatcagac tataaaccaa
      121 aagctattga aagtgagggt acggttgatt ccattacaac caatgaaatc aatgagtttt
      181 taacgacgtt cttcaagctc tatcctacag cgacagccag tgaactttcc tactatgtga
      241 atgacgggat attaaaacca atcggaaaag agtacatctt tcaagaactg gtaaatccta
      301 tttacaatcg taaggataat caagtcacgg tatcgctgac agtggagtat atcgaccagc
      361 agaccaaagc aacgcaggta tctcaatttg atttggtact tgaaaagaac gggagtaatt
      421 ggaagattat agaataacaa atattggtac attattacag ctattttgta atcacgtact
      481 ctctttgata aaaaattgga gattccttta caaatatgct cttacgtgct attatttaag
      541 tatctattta aaaggagtta ataaatatgc ggcaaggtat tcttaaataa actgtcaatt
      601 tgatagtggg aacaaataat tggatgtcct tttttaggag ggcttagttt tttgcaccca
      661 gtttaagaat acctttatca tgtgattcta aagtatccgg agaatatctg tatgctttgt
      721 atgcctatgg ttatgcataa aaatcccagt gataagagta tttatcactg ggatttttat
      781 gcccttttgg gcttttgaat ggaggaaaat cacatgaaaa ttattaatat tggagtttta
      841 gctcatgttg atgcgggaaa aactacctta acagaaagct tattatataa cagtggagcg
      901 attacagaat taggaagcgt ggacaaaggt acaacgagga cggataatac gcttttagaa
      961 cgtcagagag gaattacaat tcagacagga ataacctctt ttcagtggga aaatacgaag
     1021 gtgaacatca tagacacgcc aggacatatg gatttcttag cagaagtata tcgttcatta
     1081 tcagttttag atggggcaat tctactgatt tctgcaaaag atggcgtaca agcacaaact
     1141 cgtatattat ttcatgcact taggaaaatg gggattccca caatcttttt tatcaataag
     1201 attgaccaaa atggaattga tttatcaacg gtttatcagg atattaaaga gaaactttct
     1261 gccgaaattg taatcaaaca gaaggtagaa ctgtatccta atatgtgtgt gacgaacttt
     1321 accgaatctg aacaatggga tacggtaata gagggaaacg atgacctttt agagaaatat
     1381 atgtccggta aatcattaga agcattggaa ctcgaacaag aggaaagcat aagatttcag
     1441 aattgctcct tgtaccctgt ttatcatgga agcgcaaaaa gcaacatagg gattgagcag
     1501 cttatagaag tgataacgaa taaattttat tcatcaacat acagaaagaa gtctgaactt
     1561 tgcggaaatg tcttcaaaat tgaatattcg gaagaaagac aacgtcttgc atatgtacgc
     1621 ctttatggcg gaatcctgca tttgcgggat tcggttagaa tatcggaaaa ggaaaaaata
     1681 aaaattacag aaatgtatac ttcaataaat ggtgaattat gtaaaattga taaggcttat
     1741 tccggggaaa ttgttatttt gcaaaatgag tttttgaagc taaatagtgt tcttggagat
     1801 acaaagctat tgccacagag agagagaatt gaaaatccgc tccctctgct gcaaacaact
     1861 gttgaaccga gcaaacctca acaaagggaa atgttacttg atgcactttt agaaatctcc
     1921 gacagtgacc cgcttctaca atattatgtg gattctacga cacatgaaat tatactttct
     1981 ttcttaggga aagtacaaat ggaagtgatt agtgcactgt tgcaagaaaa gtatcatgtg
     2041 gagatagaac taaaagagcc tacagtcatt tatatggaga gaccgttaaa aaatgcagaa
     2101 tataccattc acatcgaagt gccgccaaat cctttctggg cttccattgg tttatctgta
     2161 tcaccgcttc cgttgggaag tggaatgcag tatgagagct cggtttctct tggatactta
     2221 aatcaatcat ttcaaaatgc agttatggaa gggatacgct atggttgcga acaaggatta
     2281 tatggttgga atgtgacgga ttgtaaaatc tgttttaagt atggcttata ctatagccct
     2341 gttagtaccc cagcagattt tcggatgctt gctcctattg tattggaaca agtcttaaaa
     2401 aaagctggaa cagaattgtt agagccatat cttagtttta aaatttatgc gccacaggaa
     2461 tatctttcac gagcatacaa cgatgctcct aaatattgtg cgaacatcgt agacactcaa
     2521 ttgaaaaata atgaggtcat tcttagtgga gaaatccctg ctcggtgtat tcaagaatat
     2581 cgtagtgatt taactttctt tacaaatgga cgtagtgttt gtttaacaga gttaaaaggg
     2641 taccatgtta ctaccggtga acctgtttgc cagccccgtc gtccaaatag tcggatagat
     2701 aaagtacgat atatgttcaa taaaataact tagtgtattt tatgttgtta tataaatatg
     2761 gtttcttgtt aaataagatg aaatattttt taataaagat ttgaattaaa gtgtaaagga
     2821 ggaattaatt atgaagtgta atgaatgtaa cagggttaaa tacaaccaaa ttttaatttg
     2881 gctttgcgtt ttatcttttt ttagcgtatt aaatgaaatg gttttgaacg tctcattacc
     2941 tgatattgca aatgatttta ataaaccacc tgcgagtaca aactgggtga acacagcctt
     3001 tatgttaacc ttttccattg gaacagctgt atatggaaag ctatctgatc aattaggcat
     3061 caaaaggtta ctcctatttg gaattataat aaattgtttc gggtcggtaa ttgggtttgt
     3121 tggccattct ttcttttcct tacttattat ggctcgtttt attcaagggg ctggtgcagc
     3181 tgcatttcca gcactcgtaa tggttgtagt tgcgcgctat attccaaagg aaaatagggg
     3241 taaagcattt ggtcttattg gatcgatagt agccatggga gaaggagtcg gtccagcgat
     3301 tggtggaatg atagcccatt atattcattg gtcctatctt ctactcattc ctatgataac
     3361 aattatcact gttccgtttc ttatgaaatt attaaagaaa gaagtaagga taaaaggtca
     3421 ttttgatatc aaaggaatta tactaatgtc tgtaggcatt gtatttttta tgttgtttac
     3481 aacatcatat agcatttctt ttcttatcgt tagcgtgctg tcattcctga tatttgtaaa
     3541 acatatcagg aaagtaacag atccttttgt tgatcccgga ttagggaaaa atataccttt
     3601 tatgattgga gttctttgtg ggggaattat atttggaaca gtagcagggt ttgtctctat
     3661 ggttccttat atgatgaaag atgttcacca gctaagtact gccgaaatcg gaagtgtaat
     3721 tattttccct ggaacaatga gtgtcattat tttcggctac attggtggga tacttgttga
     3781 tagaagaggt cctttatacg tgttaaacat cggagttaca tttctttctg ttagcttttt
     3841 aactgcttcc tttcttttag aaacaacatc atggttcatg acaattataa tcgtatttgt
     3901 tttaggtggg ctttcgttca ccaaaacagt tatatcaaca attgtttcaa gtagcttgaa
     3961 acagcaggaa gctggtgctg gaatgagttt gcttaacttt accagctttt tatcagaggg
     4021 aacaggtatt gcaattgtag gtggtttatt atccataccc ttacttgatc aaaggttgtt
     4081 acctatggaa gttgatcagt caacttatct gtatagtaat ttgttattac ttttttcagg
     4141 aatcattgtc attagttggc tggttacctt gaatgtatat aaacattctc aaagggattt
     4201 ctaaatcgtt aagggatcaa ctttgggaga gagttcaaaa ttgatccttt ttttataaca
     4261 ggaattcaaa tctttttgtt ccattaaagg gcgcgattgc tgaataaaag atacgagaga
     4321 cctctcttgt atctttttta ttttgagtgg ttttgtccgt tacactagaa aaccgaaaga
     4381 caataaaaat tttattcttg ctgagtctgg ctttcggtaa gctagacaaa acggacaaaa
     4441 taaaaattgg caagggttta aaggtggaga ttttttgagt gatcttctca aaaaatacta
     4501 cctgtccctt gctgattttt aaacgagcac gagagcaaaa cccccctttg ctgaggtggc
     4561 agagggcagg tttttttgtt tcttttttct cgtaaaaaaa agaaaggtct taaaggtttt
     4621 atggttttgg tcggcactgc cgacagcctc gcagagcaca cactttatga atataaagta
     4681 tagtgtgtta tactttactt ggaagtggtt gccggaaaga gcgaaaatgc ctcacatttg
     4741 tgccacctaa aaaggagcga tttacatatg agttatgcag tttgtagaat gcaaaaagtg
     4801 aaatcagctg gactaaaagg catgcaattt cataatcaaa gagagcgaaa aagtagaacg
     4861 aatgatgata ttgaccatga gcgaacacgt gaaaattatg atttgaaaaa tgataaaaat
     4921 attgattaca acgaacgtgt caaagaaatt attgaatcac aaaaaacagg tacaagaaaa
     4981 acgaggaaag atgctgttct tgtaaatgag ttgctagtaa catctgaccg agattttttt
     5041 gagcaactgg atccaggaga acaaaaacga ttttttgagg aaagttataa attattttcc
     5101 gaacgatatg gcaagcaaaa tattgcttat gcaacagttc ataatgatga gcaaacccct
     5161 cacatgcatt taggtgttgt gcctatgcgt gatggaaaac tgcaaggaaa aaatgtgttt
     5221 aatcgtcaag aactgttatg gctacaagat aaattccccg agcatatgaa aaaacagggt
     5281 tttgagttga agcgtggtga acgtggctct gaccgtaaac atattgagac agctaaattt
     5341 aaaaaacaaa ctttggaaaa agagattgat tttctagaaa aaaatttagc agttaaaaaa
     5401 gatgaatgga ctgcttatag cgataaagtt aaatcagatt tagaagtacc agcgaaacga
     5461 cacatgaaaa gtgttgaagt gccaacgggt gaaaagtcca tgtttggttt gggaaaagaa
     5521 ataatgaaaa cagaaaagaa accaaccaaa aatgttgtta tatcggagcg tgattataaa
     5581 aacttagtga ctgctgcgag agataacgat aggttaaaac agcatgttag aaatctcatg
     5641 agtactgata tggcgagaga atataaaaaa ttaagtaaag aacatgggca agttaaagaa
     5701 aaatatagtg gtcttgtaga gcgatttaat gaaaatgtaa atgattataa tgagttgctt
     5761 gaagaaaaca agtctttaaa gtctaaaata agcgatttaa agcgtgatgt gagtttaatc
     5821 tatgaaagca ctaaggaatt ccttaaggaa cgtacagacg gcttaaaagc ctttaaaaac
     5881 gtttttaagg ggtttgtaga caaggtaaag gataaaacag cacaattcca agaaaaacac
     5941 gatttagaac ctaaaaagaa cgaatttgaa ctaactcata accgagaggt aaaaaaagaa
     6001 cgaagtcgag atcagggaat gagtttataa aataaaaaaa gcacctgaaa aggtgtcttt
     6061 ttttgatggt tttgaacttg ttctttctta tcttgatata tatagaaata acgtcatttt
     6121 tattttagtt gctgaaaggt gcgttgaagt gttggtatgt atgtgtttta aagtattgaa
     6181 aacccttaaa attggttgca cagaaaaacc ccatctgtta aagttataag tgaccaaaca
     6241 aataactaaa tagatggggg tttcttttaa tattatgtgt cctaatagta gcatttattc
     6301 agatgaaaaa tcaagggttt tagtggacaa gacaaaaagt ggaaaagtga gaccatggag
     6361 agaaaagaaa atcgctaatg ttgattactt tgaacttctg catattcttg aatttaaaaa
     6421 ggctgaaaga gtaaaagatt gtgctgaaat attagagtat aaacaaaatc gtgaaacagg
     6481 cgaaagaaag ttgtatcgag tgtggttttg taaatccagg ctttgtccaa tgtgcaactg
     6541 gaggagagca atgaaacatg gcattcagtc acaaaaggtt gttgctgaag ttattaaaca
     6601 aaagccaaca gttcgttggt tgtttctcac attaacagtt aaaaatgttt atgatggcga
     6661 agaattaaat aagagtttgt cagatatggc tcaaggattt cgccgaatga tgcaatataa
     6721 aaaaatta
//
LOCUS       DAFEPQ010000100         6644 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.104, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000100
VERSION     DAFEPQ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.104"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(1..859)
                     /locus_tag="LP543_002513"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="LP543_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="LP543_002514"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="LP543_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBL2322641.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="LP543_002515"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="LP543_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="LP543_002516"
     CDS             complement(2143..2373)
                     /locus_tag="LP543_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HBL2322642.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="LP543_002517"
     CDS             complement(2603..3421)
                     /locus_tag="LP543_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL2322643.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="LP543_002518"
     CDS             3582..4271
                     /locus_tag="LP543_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBL2322644.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="LP543_002519"
     CDS             4285..5787
                     /locus_tag="LP543_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2322645.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="LP543_002520"
     CDS             5800..6276
                     /locus_tag="LP543_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL2322646.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="LP543_002521"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="LP543_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAFEPQ010000101         6521 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.105, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000101
VERSION     DAFEPQ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.105"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(<1..224)
                     /locus_tag="LP543_002522"
     CDS             complement(<1..224)
                     /locus_tag="LP543_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBL2322647.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRDINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWK"
     gene            355..807
                     /locus_tag="LP543_002523"
     CDS             355..807
                     /locus_tag="LP543_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322648.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            821..1510
                     /locus_tag="LP543_002524"
     CDS             821..1510
                     /locus_tag="LP543_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322649.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1635..3986
                     /locus_tag="LP543_002525"
     CDS             1635..3986
                     /locus_tag="LP543_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HBL2322650.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            4066..4461
                     /locus_tag="LP543_002526"
     CDS             4066..4461
                     /locus_tag="LP543_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322651.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4786..5403
                     /locus_tag="LP543_002527"
     CDS             4786..5403
                     /locus_tag="LP543_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2322652.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5444..5743
                     /locus_tag="LP543_002528"
     CDS             5444..5743
                     /locus_tag="LP543_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HBL2322653.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5777..5944
                     /locus_tag="LP543_002529"
     CDS             5777..5944
                     /locus_tag="LP543_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL2322654.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5989..6513)
                     /locus_tag="LP543_002530"
     CDS             complement(5989..6513)
                     /locus_tag="LP543_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2322655.1"
                     /translation="MDKLFQEKASGKDTERPEFQKLLNYIREGDCVIVTSLDRLGRDY
                     EDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSLLSYIAQNEREKIR
                     ERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQGIPVAEIAKENGI
                     SRPTIYKIKKANDL"
BASE COUNT         2234 a          912 c         1284 g         2091 t
ORIGIN      
        1 attttccatg aataaaagga ttttttattt ttctttttcc aaatttgata gagtagtttg
       61 ccatattctt gcacccaacg ataaatcgtc gtatgagaaa cgttaatgtc acgatcatat
      121 aagatttctt gaacttcacg atagctaagg ttataacgaa gatagtagcc cacggctaca
      181 ataatcacat cctgctgaaa ttgctttcct ttaaaatgat tcatcgttat tcctcctgct
      241 atctttttct attattctat cttatttgat agtagattta aaactttgca acagaaccaa
      301 ttatcttatc gtagatatat attataaatt taataagaaa gcgaggtgag aatcgtgaaa
      361 aaaacgatac taagtatttt ttcagtattt acaatattaa ttgggggcta ttttcttttg
      421 aatttagatt ctgttaattc aaacgctcaa ataaaagatc aaagagtagc tgaacataat
      481 aataaaaaaa tgagtttgac caaaaatatc tattctatgt cagatgaaga attgtataaa
      541 gaatttggtg atttatccgg ggaacagtta gaagaaagta ttccaggaat tagtgactca
      601 gataaaattg ttgtcttgtc tgctggtggg caattaatgg gagatggcaa atccgaatta
      661 gttagttata aacaaaatca cagacagttt attgatgtta atactgattc caacgctatt
      721 tcgattaagg atctaaaaga gcttttaaat agtaaaagaa atgaattaaa gcagataaaa
      781 gaaactaatt cagtggaatt agagtaaagg aagattcatt atgaaaaaga tttttggtgt
      841 ttctgttggg ctacttatga tgttaacatt tggaaatgca gtatatgctg ctgagaaaga
      901 agttatttca gatccagagc agtacgcctc acgatacttt gctgattttg aagaagaaga
      961 taaagtactt gcattgccag aaggtggttt tttaaaaggg gaagctaaaa ttgtatctat
     1021 ggataatcca gatataattc aaggttcata taattcagaa actgatcctg cttcagtttc
     1081 agtgacagaa gcaaaaaaaa ttgttgctga aaaattcaaa aatgatattg aaactactcc
     1141 aattatgcct cgaggagcat ttccacccga tgttggtact agcggacacc ttcttgtact
     1201 agcaggagga ggaagctata aatcacagcc atttagtggt gcgggatggc gttttggagg
     1261 tatgggtgta aaaccagctg atggtacagg caacttttta cgttggaaat cctacattga
     1321 cgatggaaga gtaggaaatg taaatcaagc caacgcaact aaatctggtt ctattcaagg
     1381 aatggcattg tatgctcgtg aaggattcaa atactttgca ccaaacgggc ctggttggac
     1441 aatgaatata ggtatttatt acacttataa tcctgttgca ggtacgtatt atgttgttca
     1501 aaatatttaa taaaacagca caaagagatt tctttgtgct gttttattat ttttagttgt
     1561 tgacaaacac taaaatataa aatataatca acacataaac gaacgtatgt tcggcaagaa
     1621 agcaggggat ttttatggat gagatggttt taagtactca gaaatggcta aacaagaaat
     1681 acagtaatgt tacagggttt gataaggttc cagaaaatgg gcgaacaggg tggcctacaa
     1741 tctatggact tattgaagga ctacaagtag aacttggtat tactaatcta gtagctaatt
     1801 ttggacctac cactgaaaaa atgtacgata atcaggttac tcctaaatgg ggcaaaaatc
     1861 tacctaaaaa tattgtattt cttattcaag gtgccttttg gtgtaagggg attaatccag
     1921 gtggcttcga tggggtatat actccatatt tagatactgc tgtaaaagag ttgcagactg
     1981 atgcagggtt taatggaagc gctgtaactg gagttttgga tgcgaaatgg gccaaagcgt
     2041 tatttgatat gagtgctttt gttcttgttc ccggcggaga tagtaaaatt agaaaaatgc
     2101 agcaatggtt aaatgtaaat tatttagaat acacgggaat aatgccttgt gatggtattt
     2161 atcaaagaaa ttcaaatcaa gcattaattt ttgcattaca agctgaacta ggatattctc
     2221 ccagtgaagc aacagggtct tatggtaatg ggacaactag taaaacttat cctgtgtctg
     2281 aaggtaactc gggaaactat gtaagaataa ttcaatatgg attatatgtt aatggttatt
     2341 ttgaagacgg tacattcgat ggtattttca ctaaatatat gggtttagaa gtactggcat
     2401 tccgaaaatt tatggtattg cctgaatata ctgaaatagc agatgttgtt gtaataaaag
     2461 gactattgtc tagcgctgga gatattagaa gatctgcaga tggcgcagat acttctacgc
     2521 aattaactag atctcaaatc caaacattgg ttgataatga tataaaaata gttggtagat
     2581 atcttactgg tactgttgga attggaaaag atgaacgaaa taagtatcta actacagagg
     2641 aattgaataa tattttttct aaaaatttat ctgtgtttcc tatctatcaa gatggaggtg
     2701 cagcattagc ttattttact tatgatcgtg gcttatcaga tggtaagaag gctatagatg
     2761 cagcaaaaaa tctaaatata ccattaacaa cagttattta ttttgctgtt gatctagaca
     2821 tgttagggga agaaattctt gcatatgctg gagaatattt taagggggta tctgctgtaa
     2881 tgtcatatag cgggtatcaa acgggtgttt acggaacaag aaatgtttgt tctcaaataa
     2941 tcaataacgg gtatgcttcg tttgcatttg tttcagatat gtcgactggt tatagtggga
     3001 atttaggttt tcctatgcct agacaatggt cttttgatca atttgttgaa tttaccatag
     3061 gaagtggcaa cggagctgtt ggtatcgatt taatcgctac atcgggtaga gatagcggat
     3121 ttaatgagtt atcaaatgat actaataatg ataattacat tgccaaatac aatcagaagg
     3181 ttataaatca gatggtacgt gcgtatcaat atctatcaca agctggtctt gataatcctt
     3241 ggaaccctca tttgacattt tatagatatg taaattattc tggtttgtca tgggacatca
     3301 tttcttctcc tgtaacagag catgatagaa aaatttatga tgaatatagg aataaattaa
     3361 ctaatagcga gggcctgtat aattatttta tagatcctaa tactggttca ataataggtt
     3421 tgcctcactt aattgtgacg ttacaatcac agatgtttct agccgacaca atcaatgata
     3481 gtgtttctga ctttgcaggt tggttaggag atttaatgac ttgctggggt gaagttaaga
     3541 aattaggaat acaaatggag caaggtgtat ttgcattagt aggtacggct gcaggtggaa
     3601 catttagtct agaagattta taccaagatg ttgacgcggt aaatgtttat accttagcaa
     3661 aaagaaattc tgattcaaca gctttatcgg ctttaaaaac gtactattgt gataagggtg
     3721 ctattgatag atttaatcaa tttgttaaga acagatttgg ttcttcagat aaaatcgctt
     3781 cagtaacaaa tagtttatta gaaaatgacg aaacagttga tatcttaggt gtagggataa
     3841 tttcattttt cattgataaa atagttaata attcacttgg gacagatttt acttatcttg
     3901 attttattgg agatgagggg aaagttgcaa atggattttc tgaaaaaatt attcaatttc
     3961 aagaaaatag aagtaaactt aattaagaat cgtttactat ttaaacagtg tccttttcct
     4021 ttttaaatat atacgataaa ataagtatat gaattgtaaa aggacatgat gattatggaa
     4081 gaaatagaat ggaataatct gactcaaaat aataaaaata attctggatg tggaattatc
     4141 tttatgtttg ttatagtttt gggaatagta gtacttttaa taactgtcaa gatagtagat
     4201 tactttgcga atactcctga agaacaaata cagagaaaac ttcctgagtt tgaaagagta
     4261 ccaccttatg atccaagaga taattcattt caagtaaaaa aattaaaaga gtctccaaat
     4321 gaaatgtggt ttgaagttac agttgaatca agcgacttca aagaaaaaaa gaaagaatac
     4381 aattatattg taaaggcaaa agtatcaaaa agatttattt ttaaccctaa attagaaata
     4441 tatagtgttg taatagaata atagtgttaa aaaaattgta aaagttaaag aagtcaaatt
     4501 ctgatgtgaa aaataaaata taccatggaa gacataaaag aaaaactgga taactagttt
     4561 atttaatggc agaccgttaa ggcctgccat ttttatgtat tttagcggaa taaatataat
     4621 catgcgggag tgtaaactta ctgtttcaaa aagtatactt tttgacatgc cttgattttc
     4681 tttgtttatg tatggcataa taaccacata atacaatttg aaacgtttct gatttaatct
     4741 gattgttttg aaacaatttt agtgaaaaaa gggtagataa aaacgatgag aaaatttttt
     4801 tatatccggg tcagtagtta tgatcaatcg atcgaacgac aactgatttc cgcgaaggaa
     4861 ttaggaatcc ctgaagaata tatatttgta gaaaaagcca gcggcaaaga ttttaaacgc
     4921 cctgaatatc aattaatgaa acggatgttt cgaaaaggag atttactcta tctacaatca
     4981 ttagatagac taggcagaaa taaacaaatg attttagaag aatggcgaga attaactaag
     5041 gaaaagcaga ttgatatttc tgtgttagac atgcctctgt tagatactgc tagttacaag
     5101 caactagatg ggttagagac gcttatctct gacttaattc tgcaattact cagctatatg
     5161 gccgaagacg agcggaaacg aattaatagc cggcaaagag aaggcgtaga agctgcgaaa
     5221 caaaagggtg taaaattcgg cagaaagaaa attgctttac ctcctgaatt tcctgcgatc
     5281 tattctgact ggaaagcagg aaagataact gcagtctatg ctatggatca attgggcatg
     5341 aaacgaaata ccttttatcg acgggtgaaa gaatatgaac ataccttgca ccacttttct
     5401 tgatagatat aacagctatt agacgggaag ggagagtttg aaaatggact taacgaatca
     5461 aagggtcgtt tttactggaa cgctgcagca aatgacacga acccaagcca tcggtttggt
     5521 tcactcttta aacggccatt gtcaatcaag tgttacttca aaaacaaatt tcctggtttg
     5581 tggtcagtat tctaagtcgt tgtttgatga ttctctctat acaaaaaagg aacaaaccgc
     5641 gcgggacttg attgcgttag gtcatgagat taccatttta tcagaagtcg aattttatgc
     5701 gttaattagt caacagttga aagaatggca acggtattta taatcgttag taaaatttac
     5761 aaggaggaaa atttttatga agcgaaaatc ttatgatcgt tggggcattg aaataactag
     5821 ttttaatcga aatattgctt ttcacgtgga gtgtgactgt ggggaattag caaaaagtgt
     5881 ttatcaaaaa aattactttt actgtcctaa atgccatcgc cgatacgggg tacatggaac
     5941 atagaaacga aatagtgcgg tagaaaagcg aatgaatgta gtatatttct acaaatcatt
     6001 cgctttcttt attttataaa tagttggtcg agagatcccg ttttcttttg caatctctgc
     6061 aactggaatg ccttgcttta acatgtccac cacggtttta aaaactaagc gtttttgcgg
     6121 atctgttgag ttaatagaat actcgattgg tcgtccctta tatcggccgg ctttttgagc
     6181 caataatact ccttgccgct gacgttcacg aattttctca cgctcgtttt gtgcaatata
     6241 acttagcagt gataaaaaca tatcgaacat agcggtatcc aggagttcat ttccagtatt
     6301 aaaatctaaa aactgtgcat ctaatatttt taacgctact tttttctgtt tcataaaagc
     6361 aactgtattt tttatatctt cataatctct tcctaaacga tctagtgatg taacgatgac
     6421 gcaatcacct tcacggatat aatttagtaa tttttgaaac tcaggacgct cagtatcttt
     6481 accactggct ttttcttgga ataatttatc aatgttaagt c
//
LOCUS       DAFEPQ010000102         6448 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.106, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000102
VERSION     DAFEPQ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.106"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(95..550)
                     /locus_tag="LP543_002531"
     CDS             complement(95..550)
                     /locus_tag="LP543_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322656.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(704..1912)
                     /locus_tag="LP543_002532"
     CDS             complement(704..1912)
                     /locus_tag="LP543_002532"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBL2322657.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2024..2356)
                     /locus_tag="LP543_002533"
     CDS             complement(2024..2356)
                     /locus_tag="LP543_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBL2322658.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2504..3379
                     /locus_tag="LP543_002534"
     CDS             2504..3379
                     /locus_tag="LP543_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL2322659.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3461..4042)
                     /locus_tag="LP543_002535"
     CDS             complement(3461..4042)
                     /locus_tag="LP543_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBL2322660.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4048..5307)
                     /locus_tag="LP543_002536"
     CDS             complement(4048..5307)
                     /locus_tag="LP543_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBL2322661.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEADGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5309..6355)
                     /locus_tag="LP543_002537"
     CDS             complement(5309..6355)
                     /locus_tag="LP543_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322662.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1795 a         1334 c         1099 g         2220 t
ORIGIN      
        1 tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat aagacacatc
       61 tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc ccgtatacat
      121 cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt tcgctgattt
      181 ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat gggaagtaca
      241 caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct tcgtatttca
      301 atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt gtcgcaactg
      361 cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt tcaacaaatt
      421 cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga tcgccgatat
      481 gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc gctgtcattt
      541 ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac ctcagtttag
      601 cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt atctgcccac
      661 tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc aatttttctt
      721 tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct acgacggctt
      781 tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca gtttcttcta
      841 gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa cgatcatagg
      901 attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat tcttctactt
      961 gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct ccagtttctc
     1021 gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta gaacacttcc
     1081 acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg atcaataatg
     1141 cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa cgtaaaagtg
     1201 cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca ataaatgcat
     1261 ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca tttatacgtt
     1321 ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag gtttcttcct
     1381 ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt aaggcatctt
     1441 catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct agatcttgca
     1501 gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca ttgatcgtaa
     1561 aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga tctggtctac
     1621 ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt tcatcatttt
     1681 ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga aaaagctgtt
     1741 tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc tggttcagca
     1801 agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc tctaattttt
     1861 tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc atttttccaa
     1921 cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga ctgtttgaca
     1981 agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg tccttacgct
     2041 caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa acttcagtta
     2101 agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca ccaagttctt
     2161 ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa gtgtggttga
     2221 cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct aatggagaga
     2281 aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata ctgtataaag
     2341 aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt aacgcaaatc
     2401 ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag gaatttctaa
     2461 atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca aacgaaaaac
     2521 agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg gtcttgttta
     2581 cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct tgatcttaag
     2641 agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc cattgatttt
     2701 actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag gtacactttc
     2761 tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt tacagcatga
     2821 tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg ggatcgtcta
     2881 ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag aacaaaatta
     2941 cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac ttgcttcatt
     3001 aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg ttttcagtcg
     3061 tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg tcgtctccaa
     3121 caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg tgactttcct
     3181 ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg tcgttagctc
     3241 tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag ctttcctttc
     3301 aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc cacagacaag
     3361 attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag attccacatc
     3421 cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta tgttcactta
     3481 cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca tcagataatt
     3541 tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca taaagcattt
     3601 ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca gtatcttctt
     3661 cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat agatattctc
     3721 ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc caattcaaac
     3781 ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa aacaatttca
     3841 tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca aaattcgccc
     3901 cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa ttcaagatcc
     3961 aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa aaattcttct
     4021 tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta ataatgacag
     4081 tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt gcgctctttt
     4141 cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg gttcatgtga
     4201 tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt ctaattcatt
     4261 ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca tctgattgat
     4321 cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta aagtaagtaa
     4381 tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt ctgctcgctg
     4441 tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga cttggtaagg
     4501 attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt cttcttcttg
     4561 aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt ctcgaagttc
     4621 ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa aagctagctg
     4681 gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta cgatagctgc
     4741 ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg agattcctga
     4801 aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct ctttgaaacg
     4861 atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat caggaagcaa
     4921 cccatctgct tctttcaatt ttgcttcact cacttcaggg attccgataa cttggtacaa
     4981 atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca ctttttccag
     5041 gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta acgggatgtt
     5101 cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt ttgcttcgat
     5161 caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg ctgggtcttc
     5221 ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg ataaagcttg
     5281 caacattttt tcgctatttg tattcatttc aattccctct tatctcactg acgatttgtt
     5341 gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat ccgtctatta
     5401 tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa agaacttttt
     5461 ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca agcaagtcca
     5521 gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga ttccacacga
     5581 aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg ccattttcgg
     5641 tcagataaaa agcttgttca aacagtttac cataatttga caggttttct gcctctaatt
     5701 gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt aattgctttt
     5761 tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc attccgcaac
     5821 agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg attgtttgat
     5881 cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt ggtagctgca
     5941 aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca gggatcatcc
     6001 atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc atgatccctt
     6061 catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg acgaaatcaa
     6121 gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata taccagtcat
     6181 tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca taatagcgct
     6241 gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga ctgactagcg
     6301 tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt tccatacgca
     6361 tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa agataagagc
     6421 aaagctcacc tcataacttg gaaataaa
//
LOCUS       DAFEPQ010000103         6092 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.107, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000103
VERSION     DAFEPQ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.107"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            109..1098
                     /locus_tag="LP543_002538"
     CDS             109..1098
                     /locus_tag="LP543_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL2322663.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1080..1541
                     /locus_tag="LP543_002539"
     CDS             1080..1541
                     /locus_tag="LP543_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBL2322664.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1591..2055
                     /locus_tag="LP543_002540"
     CDS             1591..2055
                     /locus_tag="LP543_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2322665.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2069..2818
                     /locus_tag="LP543_002541"
     CDS             2069..2818
                     /locus_tag="LP543_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2322666.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2818..3648
                     /locus_tag="LP543_002542"
     CDS             2818..3648
                     /locus_tag="LP543_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL2322667.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3648..4295
                     /locus_tag="LP543_002543"
     CDS             3648..4295
                     /locus_tag="LP543_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL2322668.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKINYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4393..5169)
                     /locus_tag="LP543_002544"
     CDS             complement(4393..5169)
                     /locus_tag="LP543_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2322669.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5251..5394
                     /locus_tag="LP543_002545"
                     /pseudo
     CDS             5251..5394
                     /locus_tag="LP543_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            complement(5511..6092)
                     /locus_tag="LP543_002546"
                     /pseudo
     CDS             complement(5511..6092)
                     /locus_tag="LP543_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2055 a          851 c         1089 g         2097 t
ORIGIN      
        1 atataaaatt gttataaaaa atttttttga aaaatagtat tgcaaaataa agcaatttca
       61 tagataatag gtatcgtaaa gttatataac tttataatac ctattagagt gagggataaa
      121 gaaatgagac aactacacaa agaacagatt gaaacagcaa tggcagcgtt aaaggaaaag
      181 acaattgatc atgtatattt tacagcatgt gggggttcac aagccgtttt aatggctggt
      241 caatatctat ttgataaaga attaactgta ccttgtcatg tttatactgc taacgaattt
      301 aactatgcta taccaaaagg attatctgaa aattctttag ttatcacttg ttcccattct
      361 ggcacaacac cagaaactgt agaagcaaca aaaaaagcaa gagaatttgg agcaatgact
      421 atttgtttat ctaatgaaga aggatctcct ctatgggaag cagctgaata tcctgttcac
      481 tatgattggg gtaaagatgt tgatgctagt gatttaaata aaggaattct ttatggttta
      541 ttatttaata ttttacttgt tttagatggc tctaaaaaat gggaaacttg cttgaatgaa
      601 tttgataagt taaatgatct tgttattgct gaaaagagca aatttgaaat aactgctaaa
      661 gaatggggta aaaaccaaaa acgagataaa attatttaca caattggtag cggtattaat
      721 tacggagaaa tttactccac agcagcttgc tggttcatgg aaatgcaatg gattaattct
      781 aatgctattc attctgggga atttttccat ggtccttttg aaatcactga ttatgatgta
      841 ccattcatct tagtgaaaag tattggagat acaagatttt tagatcaacg ggtagaagac
      901 ttcactaaaa aattcagtaa taaattaatg gttttagacc aagctgattt tgacttagaa
      961 tctatagata gtgtagcgaa agaatatatt gcagctgtac ttagtggcgt tgttattcgc
     1021 caaatggtag aagcgattgc ttttgaaaga ggccattcct tagatgttag acgctatatg
     1081 tggcaaatga aatattaatt aataggaggt tgcaaaagat ggcaaatcaa ttatttcaca
     1141 ttgtgattac aacacattct accctttgtg agggttattt aaaggcaaca aaattaattt
     1201 taatgaatga cgccacaggt attagtgctg ttccatttaa agaaaatact agtccagatg
     1261 attttgagga gagtatgaaa gaaataatta gtcagcatca tttgcaacct ctaattattt
     1321 taacggattt aattggagga acacctaata atgtttctat taaacagttg aacaacaata
     1381 gaattcaaat tattgctggg attaatttac ctcttttact ggagttatta atggcccagt
     1441 cagctggtaa atctatagaa gaaatagatt ttaaggaatt actgaataac tctcaaaaaa
     1501 gtatggttca tattaattcg cttttaaaag atgtcagtta aggtggcaat ttctattcta
     1561 taaattttaa aaattgaagg aggaaataga atgattaaat tagtgcgtgt agaccacaga
     1621 ttacttcatg gacaagtaat attttcttgg actaaacagt atgatattaa ccatattatt
     1681 gtggctgata acaaagttcc aggagatcca atgtctgtga tggctttgac aattgctaaa
     1741 cctgctgatt gcaaattaga cattgttaaa attagtgatg ttaaagggat tatggaaaca
     1801 tccccaaatg ataattacat gattttaatt aaaggaccta aggaagcttt tgaattagtt
     1861 caaaatttac cagaaattaa agaaattaat tttggcggtg tgtctaagaa aagcgagtcc
     1921 aaacaatatg gaaaggcagt ctttttaact cctgaagaat tagaagccac aaaaaaaatt
     1981 cttgatcaag aagtgaaaat atttgtgcaa caagtcccat cttcaagtat tgaaaatgta
     2041 gattttagta agtaaggggg gaaaataaat gttattaaaa gccattttat taggtttagt
     2101 tggtgtggta gctgttattg actccaggtt aattggtcga caaaatattg gtagaccact
     2161 aattttgagt acacttgccg gacttgtgct aggtgacttg catacaggga ttgttcttgg
     2221 ggcatcttta gaattaattt ctatgggatt cgtctcaatt ggggcggctg gacctcctaa
     2281 tatgcaactt ggtagtatta ttgccactgc ttttgcaatt ttaactaata gtagtacaga
     2341 aacagcctta acaatagcta tcccagtggc tgtagctggt gaatttatga gtattatcat
     2401 gcgcatgata attgctcaat ttggacatgc tgcagataaa gccattgaaa ataataaatt
     2461 tagaaaagct caaattattc atatttattg gtcatttatt tttaatgctt ttgtttactt
     2521 tattccaatc tttttaacag tatattttgg tgctgacgtt gtggcagatt tagtagataa
     2581 aattccagaa atgattacag acgccttgac tgtctctgga aatatgcttt ccgcattagg
     2641 gtttgcaatg ctattaagca caatgttgag taaaaaatta tatccttact ttatttttgg
     2701 attctttatt gttgcctatt caggtctttc actaattggc gtgacaattt ttgcagctct
     2761 gattgccttt gtaatggatc aagttaaata tggaaaaaga gaagaggcgc atggttaatg
     2821 gaaaatagta cagaaaaaaa acgtttaatc acaaaaaaag atcttcataa gatgtgtctt
     2881 cgttcatgga caatcatgtg ttcgtggaat tatgaaagac aaatgcatat gggatttatg
     2941 tatgggatgg cgccaatttt aaataaagtt tataaaaatg atgaagagaa aaaaagagaa
     3001 gcttatactc gtcatatggc attttttaac tgtacgcctc aaatgactcc atttattatg
     3061 ggactttgtg cttctatgga agaacaaaat gctcaagcag aagaaggaaa ctttaatact
     3121 gaatctatct ccatgattaa aaccagttta atgggtcctt ttgctggaat tggtgattca
     3181 tttttccaag gtactttaag aattattaca tttgggattg gattgtcttt tgctaagcaa
     3241 ggtagtattt taggaccgat tttagctgtt ggcctttttg ctataccttc tattttagct
     3301 gttttttacg gcggtaaatt aggatatgag tctggcaata aataccttac taaattgtat
     3361 caagaaggtc ttatggatcg agtaatgcaa tttgcttcta tcattggttt ggctgtagta
     3421 ggtggaatgg tggctagcat ggtgccaatt gtcacccctt taaaattcac tacaggggga
     3481 acagaacttg ttttacaaga cttacttgat tccattattc ctggattatt accacttgta
     3541 tgtactttgg gaatatataa attgattaag aaaaatgtta gtacaaatat gctcttatta
     3601 ggttgtattt tggcagggtt tgttttaagt ataataggta tcttataatg attaaggctc
     3661 taatttttga tatggacgga gttttaattg atagcgagat tatgtatcaa aagtgggtat
     3721 ttaatttttt aaaacaagaa aatttaagct atcctttgta ttcttatcaa aaaaagattg
     3781 gaactacagc aagtattttt gatgatatag aaaagtacaa tgagggatgt aacagtttag
     3841 aattgaaggg aaaatttact aaatattgga aatcgcaaaa gattaattac ttggatattt
     3901 ttcctaatat actaattgaa cagcttgaat ggttaaaaaa gaaaaaaata aaaatggcaa
     3961 ttgcttcttc cagtccaatg gatactataa atgaagttgt taccacttgt aaaatagagt
     4021 catattttga ttgtttaatt agcggaagag atttgccaga aagtaaacct aatcctacta
     4081 tattccttaa agcttcagaa cagctcagaa ttccagttga agaatgtctg gtgatagaag
     4141 attcttataa tggtataaaa gctggtaaaa gagcaaatat gaaggttctt gcaattaaag
     4201 ataaacgttt ttctcaagat gtaagtctag cagatgatgt tatttacgat attaaatatc
     4261 tgcgacaaga agtacaggtg aaatttggaa tataaataag ttttttgatg tatttagaga
     4321 atattagtaa aaataaaaat catgcccaaa tctaatcaaa attgattgct ttgggtatga
     4381 tttcttttaa tatcaatcta ttactttagg atctgaatca tttttatgca ccgtatctaa
     4441 agtgatattt tctgtgactt caataacata actaattcta tcacaacgaa ctgtggattg
     4501 agaataatga aatggttgat tatttttatc tttgccaacc ttttctaaaa taaatagcgg
     4561 atctcctact atacattgca ataagtcgga aagctcaggt gttgcagata aaccgtttat
     4621 ttccataata gagctcgtag ctttgacatc atagtcatct tctagaagtt tatataatga
     4681 agtatattgg ctaccttttt caaataaagt tggaaatttt tcttctgatg cgaaaaaatt
     4741 atcaattccc ataggggcac cgtcttccca cattaaacgt ttaatattgt aaactctatc
     4801 agttacattg atttgtaatt tattagctaa aaactcatca gcctttataa ttttagaatg
     4861 taaaacagta tatctaattt tatgtcctcg ggtagaaaga gaatcgctaa atccatgaac
     4921 tccagctaat tttaaacttt cttttttttg agttacaaag gtccctttcc cccttaatcg
     4981 ttcaactgtt ccactttctg ttaaaagttt aaaagctttt cgaatggtaa tcttacttac
     5041 tccataaatt ttacataatt cttcttctgt aggtaattgc tctcctggaa gatatttttt
     5101 ttcacgaata tcgttacgta tagaagcttc tacttgtaga tataatggtc tattatcttg
     5161 aaaattcata gtacactccc taaaaagtta tacttcatta tatcacaatt tattaaaata
     5221 aaagattaag atataaatat aaatgaacta gtctttgact aaccaatgcc cgcttttgaa
     5281 agaaaacaaa ttcattctgt tttaagtaaa aatccttatg ttaccactca ttctgaaggg
     5341 gatgagcctt atcgctactt ggttgtggaa ccaagtgata agacaatttt ataaattcaa
     5401 tattaattaa aataaaaaca aacaaagtag attattttac tttgtttgtt tttttattgg
     5461 gagcgtcaat aattttgtgt aaataactcg tccttctgca aaataatggg ttactcagta
     5521 aacattgaag ctaatgtatc cgtcacttgt tggaagcctt tatggcttct atttaagaat
     5581 ttttgattgt atgtatcaaa gatggatact aggaaacgct ctagtgattc ttcattttga
     5641 aactgctctt ttctgcggct gtatttcttg atttgtttct tgaaagattc gattaaatta
     5701 gttgagtaaa tgcttcggcg gataccaggc ggaaactcat aaaaagtcaa taaatcttga
     5761 ttttttatca gtgactgcgt cactttagga taagtttttt gctacttctc aatcatactc
     5821 cctaaaaagg tatttgcctc ttcctttgag ctagcttgat aaacagcctt aaaatcatca
     5881 tagatttctt ttcgatcttt gacacgtact ttatgagcaa tattacggga aacgtgaaca
     5941 cagcaatgtt ggtatttagc tttaggataa atctggtgaa tcgtatcttt catgcctttt
     6001 aagccatcgg taataaacag taagacatca tgaacccctc tggagtaaat gtcctgtaac
     6061 aactcattcc aaacgtaggt cgattcagtc gg
//
LOCUS       DAFEPQ010000104         6065 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.108, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000104
VERSION     DAFEPQ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.108"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            832..1566
                     /locus_tag="LP543_002547"
     CDS             832..1566
                     /locus_tag="LP543_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2322670.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="LP543_002548"
     CDS             1582..2751
                     /locus_tag="LP543_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL2322671.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="LP543_002549"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="LP543_002549"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBL2322672.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="LP543_002550"
     CDS             3841..4767
                     /locus_tag="LP543_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HBL2322673.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="LP543_002551"
     CDS             4786..6036
                     /locus_tag="LP543_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2322674.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2025 a          997 c         1246 g         1797 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacag caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agtta
//
LOCUS       DAFEPQ010000105         5960 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.109, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000105
VERSION     DAFEPQ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.109"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..77
                     /locus_tag="LP543_002552"
     CDS             <1..77
                     /locus_tag="LP543_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL2322675.1"
                     /translation="GGCTLFGKGFGNYDQDIKLKVYYS"
     gene            complement(150..974)
                     /gene="nadE"
                     /locus_tag="LP543_002553"
     CDS             complement(150..974)
                     /gene="nadE"
                     /locus_tag="LP543_002553"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBL2322676.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(986..2458)
                     /locus_tag="LP543_002554"
     CDS             complement(986..2458)
                     /locus_tag="LP543_002554"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBL2322677.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3095..3457)
                     /locus_tag="LP543_002555"
     CDS             complement(3095..3457)
                     /locus_tag="LP543_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBL2322678.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            3680..4432
                     /locus_tag="LP543_002556"
     CDS             3680..4432
                     /locus_tag="LP543_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL2322679.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(4572..5495)
                     /locus_tag="LP543_002557"
     CDS             complement(4572..5495)
                     /locus_tag="LP543_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2322680.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(5510..5782)
                     /locus_tag="LP543_002558"
     CDS             complement(5510..5782)
                     /locus_tag="LP543_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322681.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         1784 a         1196 c          992 g         1988 t
ORIGIN      
        1 tggggggctg tactttattt ggtaaaggat tcggtaatta cgatcaggat atcaaattaa
       61 aagtttacta cagctgatat ttttcttttc tattttataa aacaaaaaca gccaatccct
      121 atgaagggac ggctgttttt gctttattgt tatttccaga aatcatccaa aacggaaatt
      181 ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt cgattttttc ttgtgcttga
      241 gcgggaactt cttttccttc tagataatca tcaatttcat tatatgtgac acctagtgcg
      301 acttcatctg caatcattgg tttcccatct tcaagatctg cagtcggaat ttttgtgtat
      361 aatttttccg gtgcattcaa tgcttgaagc aattgtttcc cttgtcgttt gtccaaacgg
      421 aataatggca aaatgtcggc tccaccatca ccaaatttcg tgaagaatcc agtaatgttt
      481 tctgcagcat gatctgtccc taatacggct cctgctcttt ctccagctac ggcatattgc
      541 gtaatcatac gttgacgtgc tttgatattt cctttgttaa aatcgctgat tcccacacct
      601 gcttcgctta atacttgtac ttgtgcatca acagctggtt tgatatttac tcgcagacta
      661 acatcgggct ggataaagtc aagagcagct tttgcatctt cttcatctgc ttgttcacca
      721 taaggaagac gaacagcgat aaattgatag ttttcatctt ttgtttcttc acgcatttct
      781 tccatcgtta gttgcgccaa acggcctgct aaagtagaat cttggccgcc gctgatacct
      841 agtacaaaag ttttaagaaa cggatatttt ttcaaatatg ctttcataaa gtcaatactt
      901 ttacgtattt cttcttttgg atcaattgtt ggtttgacac ctaattcatt gataatttct
      961 gtctgcaggc tcatttattc tcctcctatg cttctgtatg aagttctgct acgctttttt
     1021 tcattcgttc catgatagcc attttatggt tccagcattc agtagacaga tcgactgggt
     1081 atttttgcgg attcaagtct cgtttatact cttcccatag tgaatctaga ttttcttttg
     1141 tatattcttt gatttcgttt agtgcaggta attcgtaaac tctttttcct tcgataaaga
     1201 tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt tttattgata aacgtatgca
     1261 ctggatggaa catgaagata gcttcttctt ctcgagggtc ttcatcccat aatgtcacgt
     1321 agtctccttc agatttccca tcaaaatttc gagtgatgcg ccacacttgt tttttccctg
     1381 gagtagttac tttttctgca ttgcttgata acttgatcgt atccatcatt tttccttcat
     1441 catcttcgat cgatactagc ttgaatactg caccaagggc tggctgatca taggctgtga
     1501 tcaattttgt cccaacaccc catacatcaa ttttagcttt ttgcatcttt aaattcaaaa
     1561 tcgttgcttc atccaagtca ttcgaagcat agatttttgc ttccgtaaat ccggctgcat
     1621 ctaattgttc ccgaacacgc ttagatatat aggccatatc tccactgtcg atgcgtacgc
     1681 cttggaagtt gattttgtca cctaattctt tcgccacgcg aatcgcactt ggcacacctg
     1741 atttcaacgt atcataagtg tcgactaaga acacgcagtc tttatgggtt ttagcgtagg
     1801 ccataaatgc ttcataatca tttccataag attggactaa cgaatgagca tgggtcccgc
     1861 tagctggtat cccaaatatc ttcccagcac gtacattgct tgttgcatct gctccgccga
     1921 tatatgccgc tctagttccc cagactgctg catccagttc ttgtgcccgt ctagttccaa
     1981 attccaatag tggatcttct ccgatgacag atttgatacg agctgctttt gtcgcgatca
     2041 acgtttggaa attgaccata ttcaacagtg ctgtttctac taattggcag tgcgctaaag
     2101 gcccttccac ttggatcaat ggttcatttg caaaaactag ttctccttca cgagcagaac
     2161 gaactgttgc tttaaattca aaattttgta aatactccaa aaaaccttca ggatagactt
     2221 ctaatccacg caagtaagca atatcggaat cattgaacgt cagattctct aaataattga
     2281 ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt aaaaggcatt tctcgaaagt
     2341 aacattcaaa aacagcatgt aaatcagctc gacctaattc ccagtatgtc tgcatcatat
     2401 tgatttgata tagatccgta tgcagcgtca aactgtcgtc tgggtaagta ttattcatct
     2461 tgtattactc ctcttggcaa gttttcattt cctacccatt ataacaaaaa aaattaatag
     2521 gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt tacggatatt tcttgctaaa
     2581 aatgaactcc atctgctaaa actatccttt cttattggta tttaacctaa acgagaaaga
     2641 atacttattt tattcaatgt atccgagaca cgaagaaatt agcgttttct atttttctta
     2701 cttgataaaa taagtagatt ttcctactac aatcgtagcc gttaagatac cgatgattga
     2761 acaaatataa ccaaatgtct ctacaacata atcaatggca aaagtttttg aagcaataat
     2821 acgtctttcc ttttttgctt cttatcccca aagaaacgga tataacttat tatgtattga
     2881 atttttttgg aaaggcgaat tatgacaaga tataccttgc gttgaaaaaa ctcgcggaaa
     2941 aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc agctgtcaat agaatggaaa
     3001 caaaaatggc gagacatcaa tcagtctcgc cttgtttttt attgtataca ttagatatgg
     3061 agcaggttac cataaagatg attattagtc gttattaaat agaatccttc gtattttttt
     3121 gttctatcac tacaatatct ttatttggac gtttgaattg tattccggca cgttctagct
     3181 cctgtttcac tcctacatgg actttgacat cttctcgtgt catttttaaa acgtgatgaa
     3241 tcgcatagtc gatttcttgc cttgtgattt ttttcttcgt attgatcatc acgacttgct
     3301 ttttatcata tgtgtctacc aatagcagtt gtgttcgatc taacgaaaat aatttgtaat
     3361 aattaatagc cgtattccca aaaaagaatt ccactacttt aggataaaat gtcttaatat
     3421 ttaaattact attcacaggt gtttcgatta gcttcatctc attcctcttt tctatatagt
     3481 cgatttagat ccaatcttaa gttatgttac catctttcct agcattaagg aatcggttac
     3541 aattgaacct ttaaagacaa aaggatttac cttttatcac ttaatagtat aacataattt
     3601 catgagaaaa aaatacaatt ttttcttgac catctcgtta cgtaaaggat tatgcttaca
     3661 tttgtcagga ggaaaaataa tggagtacac aataaagaaa atgtcagaga tatcaggagt
     3721 cagtccgaga accttgcgct tttatgatga gattggactg ctaaaaccag ctaggatcaa
     3781 ttcttcaggt tatcggattt atggcaaaaa agaagtcgat cgtctgcaac acatcttatt
     3841 ttatcgaaca atggcattca agttggatga tatccaagag gtcttagaca atccatcttt
     3901 tgaccaccag aaagctttaa tcaagcatca gcaaatgttg ctggaaaaac gggcacaaat
     3961 cgataccctc ttgacaactg tccaacagac acttgatatg tacgaaggag gaagaaaaat
     4021 gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag ttgaaagaaa atgaagaatc
     4081 ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag gttacagcat ccaatgaaaa
     4141 atggatgaat atggataaag aaacgtatga agaaatgcaa actgttgaaa agcagatgtt
     4201 atttgatctc actctttact taaaagaacc agaagatgaa agcttagcag accgtatctt
     4261 ccaagctcat aagaagtggt tatcttacag ctggcctgaa tatcagccaa aagcgcataa
     4321 aggactagga atgatgtacg aagccgatga acggttcact gcatattatg acgagcgaag
     4381 tggtcatggt gcagcaaaag ctttaaatga aattatccaa aaaaaggcgt aatcttttac
     4441 atgatccaac aaaaaagtac acgtaaaatg acctaataac aaaacagcag acacccaagt
     4501 tagattttta tgctaacttt tggtgtctgc tccattcttt cacaagtttt ttcatcctac
     4561 tttaaatata ttcatcgttg aataaacgta taaggtattt tcactttatc ctttttttct
     4621 gtaagaaaca attgaaatgc catttctccg caagcttcca gatggtggtc gatggttggg
     4681 aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa tcaccactgg tgtcttatct
     4741 tgcagatagc tttgcaagat gcctgctgca accatatccg aatttgtaaa gacaccatca
     4801 actccaagct ctttaaaaaa gaagcctgct tgttttcctt tctctgcatc tatacaatcc
     4861 caaaaaacaa gtgattcatc aaaagatgga aaaagttgat gacatagctg caaagtgatt
     4921 tttgagttat aacttagcct gccacttcgc cccaacgtga tgcccaattg ctttatacct
     4981 tgctgcttca aataagtaag accttctgta attgattgtt ctcgatcaat aaagacacag
     5041 cttgcctgtt ttcccttgat ttcttcacaa aaaactagag gtccatattt ttgatatgcc
     5101 aataaatcat ctagtgtatt ggcacgagac gtaatgatca atccatcgaa agctttagct
     5161 gcaaattctt caagatacct tcgctctttt tctttctgat agcccgtcgg taaaagtgtc
     5221 actttgtaat cataatcaaa cgctgcttca atgatcccac tgatcaactg atcaaaatac
     5281 ggatgattgg tataaggcag aatcactcca atgcttttcg tttttccata acttaactgt
     5341 ctggcatttg cattagggat ataatcgact tctttcatga ccgcaagtat ttctttcctt
     5401 ttttcctcat ctacatatgg atgtccattg attacacgtg aaatagtaga aactgaataa
     5461 cctgtgagct ttgcgatatc tcgaatgttt gccactgctg cttcgccacc tatacaaaaa
     5521 gattttcttg taaatagtct accacagctg gaccgcattc aaaaaactga tacatttttc
     5581 cttcatgcga aaaactgatc cgctgcccgc cccaatatat atcttttgtc aaattttcga
     5641 tttcattcat tggtatttct tttactatat tcctattcaa cccttgaaaa ctgtacaacg
     5701 ttaatacgga tcccttgaac tcgacaaacg cataaaaata attgttcgtt gtgccatctg
     5761 ttatctctac ataagtattc attgcaagcc ctccttttga tactttgagt atagtgtatg
     5821 aaacgcgtct catagcaagg agtttgtttt gccaaagaaa agttttattc ctctttcatt
     5881 attaccaaag tctcttattt taaattgaat acattataat gatactgtaa atatatcttt
     5941 attattagtg aatggatatt
//
LOCUS       DAFEPQ010000106         5907 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.110, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000106
VERSION     DAFEPQ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.110"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(95..667)
                     /locus_tag="LP543_002559"
     CDS             complement(95..667)
                     /locus_tag="LP543_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL2322682.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(674..1336)
                     /gene="rpe"
                     /locus_tag="LP543_002560"
     CDS             complement(674..1336)
                     /gene="rpe"
                     /locus_tag="LP543_002560"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBL2322683.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(1352..1867)
                     /locus_tag="LP543_002561"
     CDS             complement(1352..1867)
                     /locus_tag="LP543_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBL2322684.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(1869..2153)
                     /locus_tag="LP543_002562"
     CDS             complement(1869..2153)
                     /locus_tag="LP543_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2322685.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(2187..3515)
                     /locus_tag="LP543_002563"
     CDS             complement(2187..3515)
                     /locus_tag="LP543_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBL2322686.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(3529..3984)
                     /locus_tag="LP543_002564"
     CDS             complement(3529..3984)
                     /locus_tag="LP543_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2322687.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(3987..>5907)
                     /locus_tag="LP543_002565"
     CDS             complement(3987..>5907)
                     /locus_tag="LP543_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2322688.1"
                     /translation="KLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLEDYVVNYQVTI
                     ERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFGDKPVSLTHL
                     SQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAIKQLVFYYAN
                     DMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLISTLVIQCNRLL
                     KGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLCRQLFAHRVT
                     NNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLRLKKGIKIQN
                     PLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIEDQSKANNII
                     IICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISSLSFDAGGIP
                     LVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKFVQSSLVKTG
                     VQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALPHANPSMVIE
                     SNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQKEMVDRLLQV
                     HDSEKLLRIFQQKGE"
BASE COUNT         1919 a         1147 c          836 g         2005 t
ORIGIN      
        1 aattattaag ttatgtctaa tcaaataaaa aaaccattct catatttgct gaataattag
       61 catttagaga atggtttttt atctttaaaa tctactactc aaaatctgca tgtcgagcaa
      121 acatcgtttc tgagctttca cctgttttat ccattaaaga taacactaaa ctatcaaagg
      181 tgagaaatgc taattgttca aaacttgaac ccataggttg tgaaaaagta tcttcttctc
      241 gttgattttc atctttggta atacctggta atacaagaac agaatctgct aattgaccaa
      301 tcgacgagtc ttttttcatg gtaactaaag caatgcttac accactttga tttgctttgg
      361 ccgctaaatt ttttaaacta cctgtttcac ctgagccaga acagataatt aataaatctc
      421 caggttgcga atgtggtgaa ctaatttcac cgacaaggct gactgaaaaa cctaaatgca
      481 ttaagcgatt cgtaaatgct tgcatggcaa ttccagagcg tcctgcacca gctagaaaga
      541 tgtgtttagc ttgaacaatt ttctcactaa attgttctaa ctctacagaa ttaatacgtt
      601 ttgagttatt ttgtaattct aagataatcg cttctaaata gtttttattt tggtctttat
      661 tggtcatgat cttttacttc acttcctcta atgattgatt tagtttatca atttgttctt
      721 gcggggtatt tttcccattg aagatgaaac cgcctgatac aaaaatatcc gcccctgctt
      781 tagaacaaac tttaatcgtt tgattatcaa tcttgccatc tacttcaata tcatacgtat
      841 aatcattttg tttgcgtatt acatctaact gttcgatttt aactactgtc tcttgaataa
      901 aatgcttatt ctctgttcct ggattgattg tcattactaa tacttgatcg actaaagata
      961 atactggttc aataaaactg actggtgttg caggattaaa agtaatccct acttttgctc
     1021 ctgcttcctt gactaactga atgaccttca ttgcgtgttt ggtagcttca atgtggaagg
     1081 taacaatatc tgctcctgct tctacaaatc ttgtgacaat tttttctggt gtatcaatca
     1141 tcaaatgaac atcaaacaca acgtctgtta gtggacgaat atctttaata acacttggtc
     1201 caaaagccat atctggtaca aaacagccat ccattacatc aatatgcaaa taatcaacat
     1261 tcttttcttt taagatttca aataactctt taacttctgc gataggtgca tttaacaatg
     1321 atggagcaat tgtcattctc tattcccccg tttattctcc cataatcatg agattgattg
     1381 ttctagttcg ctctcttagt tgatcccaat cgacaaaata gacacggccc atttctccta
     1441 ataaaagttt accttcttga acaattaaag tcattgagtt ggttccataa atagatgaac
     1501 gtaaatgtgc atcggtattt agcatgaccc atttttctgc agggtaattg ggatcagata
     1561 atcttgttcc aaaatcaatg tgttttggcc ccggactgtt gtattgattt tcactcgtca
     1621 tttttggaat aatcgtttcc attacattgt tgatgtctac atgtaaataa tcatcaccaa
     1681 aaaagtttgt gtcatgcatc gtttcttcaa aaaagagtga gcaagttgta tgcgggctcg
     1741 aaattactaa cattccattc tcaatacctg ctgtatcaac tgtatctaaa agttcttttc
     1801 gaatatcatg atacgaagga cgccctgaaa ctgtttcaat agtcaaagtg tcatgatgaa
     1861 aacgcatatt aaacacctaa tctttctttt acatctgcat aaatggcatc tgtaccaata
     1921 cctgttaata agcccaagcc attcgttact ttagctttaa ttacatcagg gacaattgtt
     1981 gtacttacta cagcatcata ttcatcaatt ttatttagct catcagcaat tttagattga
     2041 taaattttca cttgatctgt taatccttct tcttccaacc attttgataa ttttgctact
     2101 gcaattgtag aagtagcatg acctgctcca caaataacta ataatttttt catattgatc
     2161 tcttctttct tattttaatt ttatgattaa gctttcttca ctttgttcat gtagatagaa
     2221 gcacctacta aaacaactcc taatccacct attccaatta caccaaagta agatgctaat
     2281 aaaacaaaca accctactgg ccataatcca cataacaaca tagttactaa tcctgctgtc
     2341 cccacatcga aacttgctaa tgtaaacaca tcagtgataa ttggtgccaa ccatgttgcc
     2401 atatataatg ttgtaacaac atacaacgaa catcctacta atgatctcac aatatttcct
     2461 ttaaaataag gaatcattaa acagatggcg aaagcaaaga atgctaaatc tccaaaaggt
     2521 aatactttat ttcctggaag aattagtgct agcaataatg agattggtac aagcaataga
     2581 gaagttgaca taatagttgg atgacccaca gttaacgctg catccattcc aatgtttact
     2641 tggcgaccat ttaacttttt attcgtaaat tctttcgccg cttctgcaat tggcattaaa
     2701 ccttccataa acattgctac cattttaggc ataatcttca tcactgccgc agtcataatt
     2761 cctaagtttc caattcctgc tacgtcatag cctgccataa tacccactac aataccgata
     2821 attaggccaa taacaactgt gtcacctaaa acaccgaaac gttttgttaa tgtttctgca
     2881 tctgcatcta ttttattaaa tccaggaata cgatcaaaaa cccaatttac tggtttggca
     2941 aatagtactc ctgataatcc catcatatgt gtgatagaga ttcctggata gccgaaaaat
     3001 tcgctaacag ttttttgtgt acgatctgct aagaaccatt gtaaaattac tgcgacaatc
     3061 gcgccaatta agccaaacca aaagtttccc gtaacagatt gagtaatcat acctaggaaa
     3121 attgcgaacc aaaagttcca aatatcaaca ttcaatgttt ttgtccaacc caaaaccaca
     3181 aatatgaagt taagaattaa tgaaattgga atcattaaaa ttcccaatga tgtagagtaa
     3241 gccaaaggtc caccaacacc tacgccaata tctaggatat tcaggccacc accgtattgt
     3301 tcccccattt gttgaattgc tgttcctaaa ccatctgata ataaacttaa aactaatcct
     3361 aaaccaatag aaccgatacc aacagtaacg ccggaaacaa atgctttacc aggttttaat
     3421 ccaaataata tcccgattac aaaaataatc agtggtaaaa agacgtttga ccctaatcca
     3481 ataaaccatt caaaggcatt taatacaata tccatctctc tattcctcct aataaactcc
     3541 tgcagaatgt actaactcaa taaatgcttc tttcgtggta catgcaagta attcatttac
     3601 tttttcttca ttttgaaata ggctaactaa gttttgtagt gtatcaattt gttcatgagg
     3661 ttgtgacatt gccaacataa agactaaacg tacaggtatt tcatgagatt tatctgtcat
     3721 atctgaaaat accagtggtt cctttaatga ggcaaaacag atttgagagc tatttacatg
     3781 tgcggaatct gtatgtggga tagcaaaccc aatttgattt acttctaatc cagttggaaa
     3841 ttcttgttca cggttggtga taccttctaa gaaactatct ttcacgcaac cagcgttttt
     3901 taattgttca gacattactt gaaacaattc tttttgatct ttcacttcta attgaaataa
     3961 acataattct ttatctagat acatttttat tctccttttt gttgaaaaat tcttagtaat
     4021 ttttcgctat catgaacttg taacaagcga tcgaccattt ctttttgccc aataaattgg
     4081 taaagttctt catatactcc tctaatctca gatacatctt cttcagctaa actaaccata
     4141 atgactaatt tgacttttcg atcgccccaa ttaatcgcat tttttaatgc tacaataaaa
     4201 atgttggatt caatgaccat tgatggattc gcatgcggta aagcaacacc actttctaaa
     4261 ttagtattcc ccattttctc tctagagaag atagaagcac gaaactcttc agtaactaat
     4321 gaattggctt ctagttcgga aatcatcaaa tccaaacatt cttctttgct ttccacctgt
     4381 actcctgttt ttactaaaga tgattgaaca aatttagata tggtgggaac tttaaccgct
     4441 ttaggaatca gttcattttg aataaatcgc gaaggttctt ctattaaata tttagtataa
     4501 gcttccataa tattcatata atctgtttta cttaccaatg gagataccct tactaagggt
     4561 ataccacctg catcaaagga aagagaggaa ataatcaaat caacttcccc aatttcagct
     4621 aaatgaaact tatctaaagt tgatatttct acattatctt ttgctggtaa aaaccgtcga
     4681 actttattgt aaattaattg cgcagaagaa ataccgtatt ggcaaataat gatgatatta
     4741 tttgctttgg actgatcttc aatagcaatc tgaaaatgaa tcaaaattaa ggaaacttca
     4801 tcgtccgtta aagtcacatc aaattcttct tcaaggatgg acatggcgta ccatactatg
     4861 ctaaagagtt ctgaatattg agatttgata ttttctaata acggattttg aattttaata
     4921 ccttttttta aacgaaggac cattgctggc acatgataca ataaggattg atataatcga
     4981 tcattttgag tcaaatcaat tttttcaatc tcactcattc tttcaatcaa tttatgaacg
     5041 ctcatagcgt actcttcatt gctggttttt aaattatttg tcacacgatg tgcaaatagt
     5101 tgacgacata gatattcttt atctgcgtca taaaatggaa gattcaattt tgccttcaat
     5161 tcttcaatta aaccatttgc cacaatgtac gtttccatat agcgaatgct attgaagagg
     5221 aattcttctt caacagttaa atgatgacct ttcagtaaac ggttacattg gataactaaa
     5281 gtagaaatca atgaccgaac atagtaatca gaaacctttt cagtcagttc actgtaatca
     5341 ttgaatagca aatcatgaat gacactaata acttttgaat caaacaacat atttaaaact
     5401 tgaaaaaatg tttttgattc catgtcattc gcataataga atacaagttg cttgattgct
     5461 cgttgaattg cttgttcatt accagtaaaa ctaattcctt ctgcagatga ctgcaactta
     5521 acttctccac tgccaataat tttattgata attctcaggt cattgtataa cgatgtttta
     5581 ctaaccataa aactttgaga caaatgagtt aaagaaacag gcttatcacc aaacaaaact
     5641 tctttaatga tctcgacacg tcttttttct ggagaataaa tatctttttc aagataattt
     5701 tctaccgatg aaatcaaatg ttcaacgttt ttcctatcac ctatgagttt gatacccaaa
     5761 gaaggtaatc gctcaattgt tacttggtaa ttaactacat aatcttccaa caattcaata
     5821 tcttgataaa tggtcttggt cgacactgcc aatttatcac taaaaaaaga agcgggttta
     5881 aagtcttctt gtcgacttaa taatttt
//
LOCUS       DAFEPQ010000107         5716 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.111, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000107
VERSION     DAFEPQ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.111"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(207..1169)
                     /locus_tag="LP543_002566"
     CDS             complement(207..1169)
                     /locus_tag="LP543_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2322689.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="LP543_002567"
     CDS             complement(1171..2610)
                     /locus_tag="LP543_002567"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBL2322690.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="LP543_002568"
     CDS             2795..4741
                     /locus_tag="LP543_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HBL2322691.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..>5716
                     /locus_tag="LP543_002569"
     CDS             5005..>5716
                     /locus_tag="LP543_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL2322692.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQ"
BASE COUNT         1819 a          983 c          991 g         1923 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa ccgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaac
//
LOCUS       DAFEPQ010000108         5548 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.112, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000108
VERSION     DAFEPQ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.112"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..155
                     /locus_tag="LP543_002570"
     CDS             <1..155
                     /locus_tag="LP543_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL2322693.1"
                     /translation="KVMQDEFGKFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTT
                     TATQSQ"
     gene            175..537
                     /locus_tag="LP543_002571"
     CDS             175..537
                     /locus_tag="LP543_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322694.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            739..4170
                     /locus_tag="LP543_002572"
     CDS             739..4170
                     /locus_tag="LP543_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBL2322695.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVKKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            4221..4958
                     /locus_tag="LP543_002573"
     CDS             4221..4958
                     /locus_tag="LP543_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL2322696.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            4968..>5548
                     /locus_tag="LP543_002574"
     CDS             4968..>5548
                     /locus_tag="LP543_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322697.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLG"
BASE COUNT         1943 a          837 c         1181 g         1587 t
ORIGIN      
        1 caaaagttat gcaagatgaa tttggtaaat tttatcatga agcaattgtt gcagaacttg
       61 aagaagactt aacagatcaa tggatgacga atctatctgc tgatgtgatt aaaaaaactt
      121 cagttgtgac aactacggcc actcaatcac agtaaaaaaa acggaggtag cgaaatgaac
      181 gaagattact caaaaattga actaaacgat ggaacaattt tgaatttaga acctaaactg
      241 aatatcaaga aattattgat gatcaataga gattttaaca cagacgagtt tgcaaaaatg
      301 actgtgggaa aaggatccat ggatatttct gttattcaag gtgcaaaggc tgtgtatatt
      361 gcttaccgcc aagcgaacat gactgattat atttcattcg atgaatttat cgataaatgg
      421 gattttgata tggctactgc cagctatatt tatcaattga tgatgttcaa acaagcacgc
      481 gatgcttatc aaaaagaatt tgaaaaagca aataaggaaa aaaagcttca aaagtaaaaa
      541 ttccaaagct cttagttgaa acgtgggtcg atgtctattc gatgttgacc gacgtttttt
      601 ctatgccttc agatttggtt ttaagcgata tctgtttaga tgacattttg caaatggctt
      661 acaacaagag tgcttatgaa ggatggaaaa actatgcaat aaaccaatcc cagaaaaact
      721 aaagaaagga ggtaaaaaat ggctaaaaag agaacagaag cagaagtaac tttcatagct
      781 aacgatgacg gattgaaatc tacgttaaaa gaaatcagtg ctgaattaac taaaaataga
      841 gcagaattaa aactagaaca agctcaatta caacagactg gttctgaatc agacaagtta
      901 ggaagtaaat tatcttcttt agaaaagcag tatgaattac aaagtcaaaa agttgaagta
      961 actagccaac gtttagccaa tgcgaaaaaa tattatggag aaaattccac cgaagttaag
     1021 aaacttgaaa gagaactgat taatcaacaa acagcgcaac aacgtttgtc aaacgaaatt
     1081 gataaaacga gtaatgcact agctcaagca aaaggcgaaa tacagacgta cgagtctaca
     1141 atgcaacagt tggatagcga acaaaaaaat gttcaagcta gtgcttctct gattgaatcc
     1201 gaatacaaaa aatggcaagc aactgctggt caatcagctt ctgaagccga gaaattagcg
     1261 aaagcccaag aatatgtttc tcaacaatct gaaaatgcgg agaaaacgat agatatccta
     1321 agacgacagt tagaagctac acagtctgaa tttggcgcta catccacaga agcaatgcag
     1381 atggaagcga agcttaatga tgctgaacgt gaatttgaag agttaggaca agctgctaaa
     1441 aatgtagata caactaactt ggacgatatc ggaagcaaaa tagatatgaa taatttaatg
     1501 gaagcttctg acgttttaag cgacattggc gataagctta cagaattagg gaaacaagca
     1561 gtggactctg ctaacagtgt aggtagttcc cagagtaaga tacaagctaa ttttggtttg
     1621 tctaaacaag aggctgaaga attaacgaat gtagccagag acatttatta taaaggtttt
     1681 ggagaatcgt tagatcagtc cacagatgca ttgattttgg taaagcgtaa tttaggagat
     1741 ttaaataatc aagatttaca aaatatcacg gaacaagcta tggtcctaga aaacaccatg
     1801 ggcgctgata tggatgaaac gttacgtggt gtaaatggct taatggtcaa tttcggcttg
     1861 agtgctcaag atgcaatgga tttaatggtt tcgggtactc aaaacggttt agataaaacg
     1921 cacgaattag gcgacaatat ggcagaatat agccaattat ggagtcaaat gggatattca
     1981 gctgatgaaa cgttcggaat gcttcaaaat ggtttagatg cgggtgctta taaccttgat
     2041 aaagtcaatg acttagttaa ggaaatggga atatcgttaa cagatggtcg atttgagcaa
     2101 aacatggata tgtttagtga aagtactaga aaagcttttg aagagtggaa aaatggcgga
     2161 ggaacacaaa aagacgttat taattccatg attcaagatt ttagcaatat ggatggtcaa
     2221 tacgaccaat taaataaagc ttcgacaatt tggtctgcgc ttggcgaaga taatgcgatg
     2281 aaagttgtcc aatctttaac tgatgttaac catacatttg atgatgttag tggatctgca
     2341 caaaaaatga atgaagattc tactactccg ttgcaagagt tgaacgggaa aatagctgaa
     2401 ttaaaggatt cattagctcc tataggcaac acaatcatag atgcactcga accagtaatt
     2461 gattttctag gaaagatggc tgatgcgttt aataatcttc cacaaccagt acaggatttc
     2521 attgttgcta taggtggtct tacagcagca tttggcatac tgttaccggc aatactagcc
     2581 gtgtcatatt tatttggtcc gatgatgctt ataattggag gaattatagc tgttatagca
     2641 ggcgttattg tggcaattaa gaactggggt gcaattactg actggtttag tggtttatgg
     2701 aaaaaattta ctgattggtt gggtgatact tgggaaagta taaaagacgg agcctcatca
     2761 gtttgggatg gggttaaaga aacctggtct ggatttgtag attgggttca agatatttgg
     2821 caaggagttt ctgattggtt tggagagcta tggagcggat tagttgaagg agcttccaac
     2881 atctggcaag gagtccaaga aacttggcaa acattcgttg attgggtttc aaatatttgg
     2941 aacggagtca aagaagtatg gtcgattatt tgggcagaca ttgtaggaat tgttcaaata
     3001 ccatggacat taataacgtc attgattcaa gctggtatta atattatcgt gggtattttt
     3061 gatgtagctg gacagttatt gggcgcagct tggcaagctg tttggacacc tatttctgat
     3121 ttccttaaaa atacttggga tactatgaca caatggataa gtatcgcttg gaatgggatt
     3181 gtaactacat tccatactat atttgatcca gtagtggcat ggtggaatgg tatatggaca
     3241 gctattagta ctacggcttc aaatatttgg aattcaatta gtgcaacagc ttctagtatt
     3301 tggaacagta tcaagaatac aatcactagc ttggtacaag cagctgctac agtaattcaa
     3361 aatatttggt caactgtatc tagttggtta ggtggaattt ggaattcaat cagctctaca
     3421 gcatcaaata tctggaatag tgtgactagt agtataagca atgctataaa cgcagctaaa
     3481 agtgccattc aaagtgtttg gaatagtata tcttcgtgga ttagcggaat ttggaacggt
     3541 atcaaaaata ctgctttgaa tctttggaat ggaattacaa gcactattag ctctaaagta
     3601 aacgatggaa aaaatgcaat ttcaagcggt tggtccaatc taacaggtat tgtttccgac
     3661 atattcaata atgttaaaag tacaattgct aacatatggg aaggcatcaa aaagactgtt
     3721 agcgctccaa ttgattggat tagagacaaa atcagtggca tttttgataa tttgaatatt
     3781 tctataccac atattccgtt accggaattt atcatggaag gcagctttaa cccgctaaaa
     3841 ggtcaaatcc cccatttgcg tgtaaaatgg catgctaaag gaggtatctt tactaaacca
     3901 actttactag gtggaatgaa cggtgtcggt gaagcaggac ctgaagcagt tttacctttg
     3961 aaaagatctg ttttgcaaga aattggtgat cgtatcttga gtagcacatc agtttcatct
     4021 agggcacaaa cgattcaacc tgtgaataac tacgaattca atttcacaat tgatggtaac
     4081 gcagatgagg ttactatgaa gcaaacaact caacaaatca ttgatagcat tacaaaaatt
     4141 caaaatgata atgcttcggc atggcgttaa acaggagagt atttctcctg tttttttagt
     4201 attaaaaagg atgtgaaaaa atgactgatt gtatacattc tataatcgat ggatttcctg
     4261 attatttgca taaattggct ttagctgaaa gaccaaccat accttctcca aaaagacaga
     4321 gagttgaaac ttctgtttta ggtaggttag gtggcttagt acaagattac tcgtttgaag
     4381 atatgtcgtt tacattgcac tataactatt tagaggatgt ggaagaccat caagcgttca
     4441 agcaatcgtt ttatatcatg cgtcattggt taaactatgc aaagaaatta gaattctctg
     4501 acgatcccaa cgtctattac gttatccaga ctatcgatat tggggatgca gaaaacgata
     4561 ttgttgaatg gggagagttc gatgtaaata ttactgcgaa accattcgca agagttcaag
     4621 aagatgtacc tataaccgta gataaaccac agtcatttaa cttgctgaat aatagtttag
     4681 aagaaagttt tccaaagatt atcatcactc cttcagctac ttcatgccag ttcatcttaa
     4741 atgattatgt gtttagtttc gaaggcttag tagcaggaac tgatgtagtc attgatagtg
     4801 atttgatgct ttgctacgaa gagcaatcgg acggagatat tttagatcgg tccaacaaaa
     4861 tgaagaccat gcaatatccg acattgcaag tggatatcaa tcattttaat tgcactggtt
     4921 tgagcaaaat acaaatttat cgtaacgggt taaggtaggt gaaatagatg atcgataatt
     4981 taataactat ttacgataaa aatgacgcga ataatttagc tgaacattta tatgatacgc
     5041 aaggtttagg cgctttgtca gactggttaa cagctactgt tagcaataaa ctaaacggag
     5101 ccgagatatt tcagggtact tattcaataa acggaactaa tgcagatttg attgtagaag
     5161 gacgtattat tcagtgttat gtagatgaaa atcgagcaaa acagcgtcta cggatttatt
     5221 atgcaaaaac ttctgtaata ggcaatacga tagaagtaaa agctgaacct attttcaatg
     5281 atataagaaa atcggtgttg aataaatatg acagcggaac agaaaagatc actgctactc
     5341 aggcatggca aaacgcaaaa gttttagcga aaccagctat cccttcgcag ttttctttct
     5401 cgtcattagt agatacgctt gctaatgtga agatagaaaa ggcgaatttt ttagaattct
     5461 ttggtggaaa agagggatct attctagatc gatttcatgg tgagtttcta aaagataata
     5521 acacattacg tcatgaaaaa agtctagg
//
LOCUS       DAFEPQ010000109         5438 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.113, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000109
VERSION     DAFEPQ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.113"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..155
                     /locus_tag="LP543_002575"
     CDS             <1..155
                     /locus_tag="LP543_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL2322698.1"
                     /translation="KVMQDEFGKFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVAT
                     TATQSQ"
     gene            175..537
                     /locus_tag="LP543_002576"
     CDS             175..537
                     /locus_tag="LP543_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322699.1"
                     /translation="MNEDCSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            540..722
                     /locus_tag="LP543_002577"
     CDS             540..722
                     /locus_tag="LP543_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322700.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            740..4060
                     /locus_tag="LP543_002578"
     CDS             740..4060
                     /locus_tag="LP543_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL2322701.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFTQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWSSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SSVFDNLNISIPHIPLPHFKLSGEFNPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAG
                     PEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQ
                     QIIDSITKVQNDNASAWR"
     gene            4111..4848
                     /locus_tag="LP543_002579"
     CDS             4111..4848
                     /locus_tag="LP543_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL2322702.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            4858..>5438
                     /locus_tag="LP543_002580"
     CDS             4858..>5438
                     /locus_tag="LP543_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322703.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLG"
BASE COUNT         1918 a          807 c         1176 g         1537 t
ORIGIN      
        1 caaaagttat gcaagatgaa tttggtaaat tttatcatga agcaattgtt gcagaacttg
       61 aagaagactt aacagatcaa tggatgacga atctatctgc tgatgtgatt aaaaaaactt
      121 cagttgtggc aactacggcc actcaatcac agtaaaaaaa acggaggtag cgaaatgaac
      181 gaagattgct caaaaattga actaaacgat ggaacaattt tgaatttaga acctaaactg
      241 aatatcaaga aattattgat gatcaataga gattttaaca cagacgagtt tgcaaaaatg
      301 tcgatgggaa aaggctctat ggatattaca gttattcaag gtgcaaaagc cgtatatgta
      361 gcttatcgtc aagcgaacat ggtcgattac atttcattcg atgaatttat agataaatgg
      421 gattttgata tggaggttgc agtagctgta tacagtacta tgatgttcaa acaagcacgt
      481 gatgcctatc aaaaagaatt cgaaaaagca aataaggaaa aaaagcttca aaagtaaaaa
      541 tgccaaagct cttagttgaa acgtgggtcg atgtctattc gatgttgacc gacgtttttt
      601 ctatgccttc agatttggtt ttaagcgata tctgtttaga tgacattttg caaatggctt
      661 acaacaagag cgcttatgaa ggatggaaga actatgcaat aaatcaatcc cagaaaaact
      721 aaaggaaagg aggtaaaaaa tggctaaaaa gagaacggaa gcagaagtaa ctttcatagc
      781 taacgatgac ggattgaaat ctacgttaaa agaaatcagt gctgaattaa ctaaaaatag
      841 agcagaatta aaactagaac aagctcaatt acaacagact ggttctgaat cagacaagtt
      901 aggaagtaaa ttatcttctt tagaaaagca gtatgaatta caaagtcaaa aagttgaagt
      961 aactagtcaa cgtttagcca atgccaaaaa atattatgga gaaaattcca ccgaagttca
     1021 gaaacttgaa agagaactga ttaatcaaca aacagctcag caaaggttat ctaatgatat
     1081 agataaaacg agtaaggcat tggctcaatc taaaggtgaa tttaaaacgt atgagtcaac
     1141 tatgaaagac ttagataatg agcaacaaca tctaaagtca agcgcatcat tagtagaatc
     1201 cgaatataaa aaatggcaag caactgctgg ccagtctgct acagaatctg aaaaattagc
     1261 aaaagcacaa gaatatgtcg gcaaacagag tgatattgct gaacaaaaaa tcgaagtgct
     1321 aaaaaaacaa ttagatgcca cacaaaaaga atttggagaa acatctaccg aagctttaga
     1381 aatgaaaact aagcttaatg atgcggaaag agaattcgaa gaattaagta atgctgctaa
     1441 aaacgttgat acctccacag tagatgatat cggcaaaaaa cttgatatgg gtaatttaat
     1501 ggaagctact gatcacttgt cagtgatcgg agataagctt attgatgtag gtagtaagtc
     1561 tattgaagca gctggaaaag cacaagctat gcaggcccaa tttaaacaag tctttggctc
     1621 tttagaaggg gaagcacagg acgccgttga gggaatggct gaagaatttg gaatgttacc
     1681 aaatacgatc aagcctgttt ttacacaata tacgtcaatg tttaaaggac ttggatatga
     1741 taccaaagaa gctatggagt tagctggtga tagtactcag ttagcagcag atgcagcagc
     1801 tttttacgat aagtctatgg atgatgctag tgaatctctt aattcattta taaaagggaa
     1861 ctacgaaggt ggggagcaaa taggtttatt tgctaatgat actcaaatgg cagcttatgc
     1921 tgttaagcat aatttgatac cagcgactga aggagcaaaa aaagccagtg aagagtcatt
     1981 gttagctgtt gaaaaagcac aatctaagta tgctgatgct attaagaaac atggtgaagg
     2041 atctttagaa gcaagagagg ctgctttaaa acttaaagat gcgcaagata aaataaatga
     2101 agaattaggc ccacaaacgc aaaaatggtc tgatttagat gaggctacca agcaagcagt
     2161 tcgagttcaa tatgctgaag atatgcaaaa attagccggt gctacaggtc aggctagtag
     2221 ggagtctgat ggtttagaaa accaaatgac aagagcaaag caagcgttag aagattttta
     2281 tgcctcattg ggtgaagata tactgcctgt gtttatcaaa gggttgcaag caggagcgaa
     2341 agctttgcaa ggattagccg aatggtggag taaacttgat gggccaatga aaaatttcat
     2401 tttagctctt ggaggaattc tagcattatt aagcacatta gctcctgtta taaccgcagt
     2461 tgttacgata gttggcacat ttggttctac agttttgctg ccaataatag gaattatagc
     2521 gggcgttgca gctgtaatag cgattgtaat tacagcgttt caaaactggg gcgcaatcac
     2581 tgactggttt agtgatttat ggaaaaaatt taccgattgg ttaggtgata cctgggaaag
     2641 tataaaagaa agtgcctcat cagtttggga tggagtcaaa gaaacctggt ctggatttgt
     2701 agattgggtt caagaaattt ggcaaggagt ttctgattgg tttggagagt tatggagcgg
     2761 attagttgaa ggagcttcca acatctggca aggagtccaa gagacttggc aagcattcgt
     2821 tgattgggtt tcaaatattt ggaacggagt caaagaagta tggtcgatta tttgggcaga
     2881 cattgtagga attgttcaaa taccatggac attaataacg tcattgattc aagctggtat
     2941 taatattatc gtgggtattt ttgatgtagc tggacagtta ttaggcgcag cttggcaagc
     3001 tgtttggaca cctatttctg atttccttaa aaatacttgg gatactatga cacaatggat
     3061 aagcatcgct tggaatggaa ttgtaactac attccatact atatttgatc cagtagtggc
     3121 atggtggaat ggtatatgga catctattag tactacggct tcaaatattt ggaactcaat
     3181 tagtgcaaca gcttctagta tttggaatag tatcaagaat acaatcacta gcttggtaca
     3241 agcagctgct acagtaattc aaaatatttg gtcaactgta tctagttggt taggtggaat
     3301 ttggaattca atcagctcta cagcatcaaa tatctggagt agcgtgacta gcagtataag
     3361 caatgctata aacgcagcta aaagtgccat tcaaagtgtt tggaatagta tatcttcgtg
     3421 gatcagcgga atttggaacg gtatcaaaaa cactgctttg aatctttgga atggaattac
     3481 aagcactatt agctctaaag taaacgatgg aaaaaatgca atttcaagcg gttggtccaa
     3541 tctaacaggt attgtttccg acatattcaa taatgttaaa agcacgatag ctaatatatg
     3601 ggaaggtatc aaaaagactg ttagcgctcc gattgattgg attagagata aaatcagtag
     3661 tgtctttgat aatttgaata tttcgatacc acatattccg ttaccacatt ttaaattgag
     3721 cggagaattc aatccattga aggggaaaat cccaacattg ggtgttgatt ggtatgcgaa
     3781 aggtagtgtg tttaattctc cgaatattat cggtgtcggc gaagcaggac ctgaagcagt
     3841 tttacctttg aaaagatctg tgctgcaaga aattggtgat cgtatcttga gtagcacatc
     3901 agtttcatct agggcacaaa cgattcaacc tgtgaataac tacgaattca atttcacaat
     3961 tgatggtaac gcagatgagg ttactatgaa gcaaacaact caacaaatca ttgatagcat
     4021 tacaaaagtt caaaatgata atgcttcggc atggcgttaa acaggagagt atttctcctg
     4081 tttttttagt attaaaaagg atgtgaaaaa atgactgatt gtatacattc tataatcgat
     4141 ggatttcctg attatttgca taaattggct ttagctgaaa gaccaaccat accttctcca
     4201 aaaagacaga gagttgaaac ttctgtttta ggaaggttag gtggcttagt acaagattac
     4261 tcgtttgaag acatgtcgtt tacattgcac tataactatt tagaggatgt ggaagaccat
     4321 caagcgttca agcaatcgtt ttatatcatg cgtcattggt taaattatgc aaagaaatta
     4381 gaattctctg atgatcccaa cgtctattat gttatccaga ctatcgatat tggggatgca
     4441 gaaaacgata ttgttgaatg gggagagttc gatgtaaata ttactgcgaa accattcgca
     4501 agagttcaag aagatgtacc tataaccgta gataaaccac agtcatttaa cttgctgaat
     4561 aatagtttag aagaaagttt tccaaagatt atcatcactc cttcagctac ttcatgccag
     4621 ttcatcttaa atgattatgt gtttagtttt gaaggcttag tagtaggaac tgacgtagtc
     4681 attgatagtg atttgatgct ttgctacgaa gagcaatcgg acggagatat tttagatcgg
     4741 tccaacaaaa tgaagaccat gcaatatccg acattgcaag tggatattaa ttattttaat
     4801 tgtactggtt tgagcaaaat acaaatttat cgtaatgggt taaggtaggt gaaatagatg
     4861 atcgataatt taataactat ttacgataaa aacgacgcga ataatttagc tgaacattta
     4921 tatgatacgc aaggtttagg cgctttgtca gactggttaa cagctactgt tagcaataaa
     4981 ttaaacggag ccgagatatt tcagggtact tatccaataa gcggaactaa tgcagatttg
     5041 attgtagaag gacgtattat tcagtgttat gtagatgaaa atcgagcaaa acagcgtcta
     5101 cggatttatt atgcaaagac ttctgtaata ggcaatacga tagaagtaaa agctgaacct
     5161 attttcaatg atataagaaa atcggtgttg aataaatatg acagtggaac agaaaagatc
     5221 actgctagtc aggcatggca aaacgcaaaa actttagcga aaccagttat tccttcacag
     5281 ttttctttct cgtcattagt agatacgctt gctaatgtga agatagaaaa ggcgaatttt
     5341 ttagaattct ttggtggaaa agagggatct attctagatc gatttcatgg ggaatttcta
     5401 aaagataata acacattacg tcatgaaaaa aggctagg
//
LOCUS       DAFEPQ010000110         5023 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.115, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000110
VERSION     DAFEPQ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.115"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            87..3119
                     /locus_tag="LP543_002581"
     CDS             87..3119
                     /locus_tag="LP543_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBL2322704.1"
                     /translation="MVENKEIRFEQDIETSLTTYGKWKKETFNESNFDPIIGLDCQKL
                     LGFVQKTQPKQWARHQRNYNNDGEVKFLQRFNQEVETHGMLHVLRNGIKDRGVRFKVA
                     YFKPASELNDETMEKYEANSFTCTRQFAYSPNNHKTIDMVLSLNGIPLVALELKNQYT
                     GQTVENAKLQFQKDRDPKELVFRFNRRFLVYFAVDHTDVYMTTKLDGKKTYFLPFNQG
                     SNGPGRVGGAGNPSNKDGYPTAYLWEKVLTRDNLMDIIQRFMNLEIKKEKQGDREVTK
                     KTLIFPRYHQLDVVRKLVADVKSHGSGENYLIQHSAGSGKSNSIAWLAYHLASIHDQL
                     NYPIFSSIIIVTDRTVLDRQLQNTIMNFDHQTGLVETIDDRKSSQDLKEAINDGRKII
                     ITTLQKFPVIYQEVDQVEGRNFAVIVDEAHSSQTGNSAQKLKAALSDKSKSLEEYYEL
                     EGEVDDVLPDAEDELVQQLVAQGKHQNLSFFAFTATPKEKTLEMFGTKQPDGHFEPFH
                     VYSMRQAIEEGFILDVLKNYMTYSTSYKIAREIEDNPELPKSEATRAIVRFQSLHPWA
                     VTQKTAVMVEQFRAVTRTAIGGQAKAMVVTASRLHAVRYMKEFKKYIANKGYNDLDVL
                     VAFSGSVVDGETEYTETKMNVTKSGENVRENQLKEAFHTDNFNILIVAEKYQTGFDEP
                     LLHTMFVDKKLKGVKAVQTLSRLNRTMPGKKDTFILDFVNDVEDIEAAFQPFYEQTNL
                     REEINVNLIYDTQSKLRKFNVYNQGDIERVMKLVKQAQKQQNERLLGRISSMFKPIMD
                     RYEEMNKDNQYQFRVTLRNFGKWYDYISQLDRLFDMELLEERIFTGYLLKFLPKETRE
                     NIDISDKVKLEYYKLRQDFSGEIHLVNKEGELVNPKSIDAGIKPPDERDSLEEIIHKI
                     NERFPNDFDEGDRVLVEILYRSFADNPDLKVVSMAKNNDAQMFEKSLFPDVFKDKVME
                     QYEKNNAAFEKMFGHDDKYFDLVYTLVARDLYKLLRTK"
     gene            3309..3893
                     /locus_tag="LP543_002582"
     CDS             3309..3893
                     /locus_tag="LP543_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBL2322705.1"
                     /translation="MSTTIEVNKQSVKQFLETGKIKKFVIPEYQRPYAWTDEQNQVLF
                     DDLAEYTANNNESTYFLGSIVAYENDHNEQEIIDGQQRITTLFLFLRAIYAKLENSCE
                     KEALFLKSQIEPALWEQDDLTGEVKPDKILIMSRVMGDEGNEEFASILVSGEADVKSK
                     SNYSKNYILIQHLLNEYATNEPLSFYRFISKKAI"
     gene            3890..>5023
                     /locus_tag="LP543_002583"
     CDS             3890..>5023
                     /locus_tag="LP543_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002404167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL2322706.1"
                     /translation="MNELFRVHLEKAVNTLLKTELTAFLDYEKYDRIGFNTGNSRNGS
                     YDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEETVIHLFRKGITMSEIAD
                     LIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAKE
                     AIHIVVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAG
                     AVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFA
                     EKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKKYSHRKEQFQ
                     NEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQPME"
BASE COUNT         1667 a          878 c         1079 g         1399 t
ORIGIN      
        1 ctgaattgat ggaacttgag agttataaaa aagctctcat ctacgaatac gtaaccggta
       61 aaaaagaagc ctaggaggtg attcatttgg tagagaacaa agaaattcgt tttgagcagg
      121 atattgagac ttcactgact acgtatggca aatggaagaa agaaacattc aatgaatcaa
      181 actttgatcc cataattggt ttggattgtc aaaaattgct aggctttgtc cagaagactc
      241 aaccaaaaca gtgggcacgt caccaacgaa actataacaa tgatggagaa gtcaagttcc
      301 ttcaacgctt taatcaagag gtagaaaccc acggcatgtt acatgtattg cgcaatggga
      361 tcaaagaccg cggtgtgcgc tttaaagtag cttacttcaa acctgcttca gagctgaatg
      421 atgaaacgat ggaaaagtat gaagcaaata gttttacctg tactcgccag tttgcttaca
      481 gtcctaataa ccataaaacc attgatatgg ttttgtcgtt aaatggtatt ccgctcgttg
      541 ctttagaatt gaaaaaccag tacactggtc aaacagtaga aaatgccaaa ctccagtttc
      601 aaaaagatcg tgatccaaaa gaattggtat ttcgttttaa tcgccgcttc ttagtctatt
      661 ttgcggtgga ccatacagat gtttatatga cgacaaagtt ggacggtaaa aaaacgtact
      721 tcttgccatt caatcaaggt tccaatgggc cgggaagagt tggtggagca ggcaatccgt
      781 ccaataaaga tggctaccca actgcctact tgtgggaaaa ggtgcttact agagacaatt
      841 tgatggatat tatccaacgt tttatgaatc tagagattaa aaaagaaaag caaggcgaca
      901 gagaagtcac aaagaaaacg ttgatttttc ctcgttatca tcagttagat gtggttcgaa
      961 aattggttgc agatgtaaaa agccatggta gtggagaaaa ttatttgatc cagcatagtg
     1021 ctggttctgg aaaatctaat agtattgcat ggctggcata ccatttagcc tctattcatg
     1081 atcaactgaa ctacccaatt ttttcatcaa ttattattgt gactgatcga acagtgttgg
     1141 atcgccagtt gcaaaatact atcatgaact ttgatcatca gacaggtttg gtagaaacga
     1201 ttgacgatcg caaatcctct caagatttaa aagaagccat taatgatggg cgtaagatca
     1261 ttattaccac tttgcaaaag ttcccagtga tctatcagga agtagatcaa gtggaaggtc
     1321 gtaattttgc tgtgattgtg gatgaggcac attcttcaca aactgggaac agtgcccaga
     1381 agctaaaagc cgctctttcg gacaaatcta aatccttgga agagtattat gaactggaag
     1441 gcgaagttga cgatgtatta ccagatgctg aagatgaact agttcaacaa ttggtagcac
     1501 aaggtaaaca tcaaaattta agtttctttg cttttaccgc tacaccgaaa gaaaagacat
     1561 tggaaatgtt tggtacgaaa caaccagatg ggcatttcga accctttcat gtttactcga
     1621 tgcgccaggc gattgaggaa ggctttatct tagatgtgct gaagaactat atgacctata
     1681 gtacttccta caaaattgcc cgtgaaattg aagataaccc agaattacca aagagtgaag
     1741 caacccgagc gattgttcgt ttccaatcat tgcatccttg ggcagttact caaaagacgg
     1801 ctgtgatggt tgagcaattc cgagcggtta ctcgtacagc tattggtggg caggcaaaag
     1861 caatggtcgt taccgcatca cgcttacacg ctgttcgcta tatgaaagaa tttaaaaaat
     1921 atatagcgaa caaaggttat aatgacttgg atgtgttggt tgctttttct ggttcggtag
     1981 tagatggaga gactgaatat acagaaacga agatgaatgt cacaaaatct ggtgagaatg
     2041 ttcgggagaa tcaattaaag gaagctttcc atacagataa ttttaatatc ttgattgttg
     2101 ctgaaaaata tcaaacgggt tttgatgaac cgttgctgca taccatgttt gtcgataaga
     2161 agttaaaggg tgttaaagct gttcagactt tatcacgact caatcgaaca atgcctggga
     2221 agaaggatac ctttattctt gattttgtaa atgatgttga agatattgaa gctgctttcc
     2281 aaccctttta tgaacaaaca aatttgagag aagaaatcaa tgttaacttg atttatgata
     2341 ctcaaagcaa gctacgaaaa tttaatgttt acaatcaagg tgatattgaa cgtgtcatga
     2401 aattggtgaa acaagcccaa aagcaacaga atgagcgctt gcttggacga atttctagca
     2461 tgtttaagcc gattatggat cgctatgagg aaatgaacaa ggataatcag taccagtttc
     2521 gggtgactct acgtaacttt ggcaagtggt atgattatat ttcacaactt gatcggttat
     2581 tcgatatgga actattggag gaacgaattt ttactggata cttgctgaag tttctaccta
     2641 aagaaacacg agaaaatatt gatatttctg acaaagtaaa gttggaatat tataagttac
     2701 gtcaggactt ttccggtgaa atccatctag tgaataaaga aggcgagcta gtaaatccaa
     2761 agtcaattga tgcaggaatc aaaccgccgg atgaacgaga ttcactagaa gaaatcattc
     2821 ataaaatcaa tgaacgattt ccaaatgatt ttgacgaagg agaccgggtg ttagtagaga
     2881 ttttgtatcg gtcatttgcc gataacccag atttgaaggt tgtcagcatg gctaagaata
     2941 acgatgctca gatgtttgaa aaatcgctgt tccctgatgt atttaaagat aaggtgatgg
     3001 agcagtacga gaagaacaat gcagcttttg agaaaatgtt tgggcatgat gataagtact
     3061 ttgatctagt atatacatta gttgctagag atttatacaa gttattacgg acgaaataaa
     3121 atttataaat aaggattaga caatacaatt tctctcaaaa ataaaggaga agttgtattg
     3181 tttgctttta agaaagccct gaaaaatttg ttacgtacta aattgtctga actctgtcat
     3241 atcagctatg tcagaataaa ggaaatagat ttatttttat aaacagtgtc gtctcggaga
     3301 tttaaactat gtcaacgaca attgaagtga acaaacaaag cgtgaagcag tttcttgaaa
     3361 cagggaaaat caaaaaattc gttattccag aatatcaacg tccttatgcc tggactgacg
     3421 agcaaaacca agtactattt gatgatttag cggagtacac agcaaacaat aacgagagta
     3481 cttacttttt aggaagcatt gtggcttatg aaaatgatca taacgagcaa gaaatcattg
     3541 atggacagca aaggataact acgctatttt tatttttgag agctatctat gctaaattag
     3601 aaaatagttg tgaaaaggaa gctttattct tgaaaagcca aattgaaccc gcgttatggg
     3661 aacaagatga tttaacaggt gaggttaaac cagataaaat tctgattatg tctcgagtaa
     3721 tgggggatga ggggaatgaa gaatttgcta gtattctagt aagtggtgaa gcagatgtta
     3781 aatcaaaaag taattattcg aagaactata ttttaattca acatctatta aatgagtatg
     3841 caacaaacga accactatca ttttatcgtt ttatcagtaa aaaggcgatt tgaatgaatt
     3901 attccgtgtc catttggaaa aagctgtcaa tacgcttctc aaaacggagt taacggcttt
     3961 cctcgattac gaaaagtacg atcgcattgg ttttaacacg ggtaattctc gtaacggctc
     4021 ctatgaccgt acggtcaaga ccgagtacgg ggaacttcat ctccagattc cgcgcgaccg
     4081 caacggcgag ttcaagcaac agactgttcc tgcttataga cggacgaatg ataccttaga
     4141 ggagaccgtc attcacctct tccgaaaagg tattaccatg tcggaaatcg cagacttgat
     4201 tgagaaaatg tatgggcatc actacacgcc ccaaaccatg tccaatatga ctaaatcatt
     4261 tacggaagaa gtcacttcat ttaaggaacg tgagcttcat gaccgttatg ccgctattta
     4321 tatggacgca acctatatcc ctttaaagcg gaaaaccgtc gccaaggaag ccattcatat
     4381 cgtagttggc attcgcccgg acggttcaaa ggaagtgttg agctatgcga ttgcaccaac
     4441 tgaatctatc acgatttggg aagagatttt aatagacctc caggaacgtg gcgtgaaaaa
     4501 cgtcctcctt ttcatcacgg atggcttaaa ggggatggca ggggccgtgc agcggttcta
     4561 ccccaaagcc cgttttcaac attgctgtgt gcacgtttcc cgtaatatca gtcacaaagt
     4621 gcgtgtcgat gatcgtaagg aagtctgtga tgattttaaa atggtgtatc aagcgtcatc
     4681 taaaaaggcg gcattggaag cacgtggtgc ttttgcggaa aaatggaaaa ccagttatcc
     4741 aaaaatggtg gaatcgatcc tttcgaacga ttacctgctc actttctatg atttcccatt
     4801 agccatacgc aagactattt actctacgaa cttgattgaa tcctttaata agcaaatcaa
     4861 gaaatacagc caccgcaagg aacagttcca aaatgaagag tcgatggaac gtttcttagt
     4921 ctcgtctttt gatacttaca accaaaaatt cctaggtcgc agtcataaag gtttccaaca
     4981 ggccgaaggc gagctcgaac aaatgctgag tcaaccgatg gag
//
LOCUS       DAFEPQ010000111         4932 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.116, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000111
VERSION     DAFEPQ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.116"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            85..972
                     /locus_tag="LP543_002584"
     CDS             85..972
                     /locus_tag="LP543_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBL2322707.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTITSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="LP543_002585"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="LP543_002585"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBL2322708.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="LP543_002586"
     CDS             2274..2720
                     /locus_tag="LP543_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL2322709.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="LP543_002587"
     CDS             2993..4345
                     /locus_tag="LP543_002587"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HBL2322710.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="LP543_002588"
     CDS             4516..4803
                     /locus_tag="LP543_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322711.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1694 a          812 c         1031 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca atcacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaga atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt tt
//
LOCUS       DAFEPQ010000112         4809 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.118, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000112
VERSION     DAFEPQ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.118"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(91..1176)
                     /gene="xerS"
                     /locus_tag="LP543_002589"
     CDS             complement(91..1176)
                     /gene="xerS"
                     /locus_tag="LP543_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HBL2322712.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3252..3770)
                     /locus_tag="LP543_002590"
     CDS             complement(3252..3770)
                     /locus_tag="LP543_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HBL2322713.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1580 a          786 c          730 g         1713 t
ORIGIN      
        1 tataaaaatg ataatgaagc ttgcctttaa agttgggctt ttatctaatt ttagagacag
       61 gcttcattat tatactattt tttactacat ttacaaagcg ttcaaagcat caattgtagc
      121 tgcattgtct atatgagtat ataaagttgt tgtatttgta cttgtatgtc ccaattgttg
      181 ggcagttaat accaatgaat ttgtctgttg atagagtcta gtagccaaag tatgacgtaa
      241 tttatgtgga ctgacttgga ctctgaatcc ttttgaatat tttgctacca tccgttcgat
      301 tgcaataccg gatatacgct tggcttctcc ttgataaatt gctagaaata aagctttttc
      361 gttttctgtt actttgtatc gttctttccg aatagctaga taattagcta aatattccat
      421 tgcaaatgct gctatattta ctgcgtctct cttatttcct tttctagtta ctaccacatt
      481 attcttttct aaagataggt cctctaaatt aatatttaca acctcggaga gtcttaatcc
      541 agatcctaaa atcaatgcat tgatcgccag gtctctttct acatctcgtt gataatagat
      601 caaagctcgt ttgctcaaag tttttttata ttcattggca atataatcta gataattttg
      661 tgtatcttcg tctagaaata attttttctc gattgcgttt gcacgggcag ctaacgtagt
      721 tttatctgtg atagttgcta cttgatccat aggattattt tctaagtatg gttttcctaa
      781 aggattattg gaacttttcg aaagatattt aaatagaacc tttaatgctg tgatatttct
      841 attgatagta gaagtagata gacctaagtt attattatct ttgagcttag tttttgttgt
      901 ctcttttttt acattttcct ttgtgcgacg tttcaaatga gatttgtaca agtttagatc
      961 ataggcagtt aactgtctta tcgaggtgat tgaagtatcg ggaacgatag actcactcag
     1021 aagccattca taaaagagct tataattttt tagatattct aaagtagttc gaggtgattg
     1081 atctttatcc tcacgatctt gaacaaaatc ttttatataa tcgggcatat gaagtatgat
     1141 tgtatctatt ttttgatata tttctgtatt tttcatgtta ttcatccttt cgataagtag
     1201 gggagaggag tatctctata attggactat accaattata gagtgttttg tcaattggta
     1261 tactctgaaa tttattaatt tatttcaaaa aagcagataa ataggtatag atttaataat
     1321 ttaaaataaa tggtatacat gaataggaaa ggaaacagaa ggaggcaatc ttaatttttt
     1381 aaattgtcta aattatatag aaagaacaat ctttctatat aattagacat aagagagact
     1441 acggttgatt tgatattaac aagcgtatat gaatgtatat acaaataaaa aaggggaggg
     1501 agaagttaaa gaaaattcag agaagtgggg cactcagtag tattaacaat ttaaaaggaa
     1561 tttcttaaag aaaatgggtt gcatcctgga gatacaattt ttttagataa agaaaaaatt
     1621 caagcaacaa ttcaaaaaaa tcttaactgc tgagattgat gtagcaatat ctcaatcatc
     1681 aacaaagtac gatcctgcct ttcaaaagct tattgataaa tgtttattca ttataaaaat
     1741 aaaagaaaaa tttatgtcag cttttttcat caaagtggta atgataattc gtctattaat
     1801 tgtatttttt aggaaatatg gtataattgt tttaacgaaa aggtaattca tgatacacca
     1861 aaaaccacta gctggcgggc tagtggtttt cttttacgtt ctattcagat taacgggtag
     1921 gcttactgtc ttttttggca ataattttgc aatcaagcca tttacagatg taatgcgcgg
     1981 atacttctgc taagattgca aaaaacatat cgaaaaggta attcataata caacctcctc
     2041 cccttgccat aataggccgg ggtagcagac agcataaata ttataccata actgaaaatt
     2101 tcattttagt acatttcaat atattgatga accagcacaa taaaacatgc catgactaac
     2161 agaaaaaaac ttattaaacc agatataact tgtaaacatc caactatata gaaaacgtaa
     2221 attttctata tagttggatt aagataacga ctatttattt tatcatattt ttactatacc
     2281 ctatactttt acttggtata tttatgaata atcgcaaatt tacccctata ctactagttt
     2341 gtaagttaga ttaataaaaa tactaaagtt atttgttctt ttattaaata cacctacaac
     2401 ttataaattg atggtattta gataacacga aagaataaaa ataacagctg atagtaaagt
     2461 ttgttgacga ttgctactca atcgaaagtg agttagtgcg tatgaacttg cttctgaaaa
     2521 cttatgaaat gaaagacctt ttacatcttg cagaaatcgg tactgggatt aaactaagca
     2581 ataaaaacta atgttcccct cattctttga acttctacta tttgatttat ttattctgat
     2641 aaatgacgat ttcaccttca ggatcttcaa atcccgtata gaatttattt aagatctctt
     2701 cagtatcagt aataggatag gcgataggcc acataccctt tgatggttag aataaactgc
     2761 tcaaaatctg tatcgtatgt tggctttagc atattaacaa aggctatctc caaattataa
     2821 accataagcg ttgaatcgtt cttaacaatt tcaattcttc ctaacccaaa gtcagaagat
     2881 ataattaccg gacttgcctt acccttcatg tgggttgttg agattccata tttaaaggga
     2941 attgtcggta ctatcggcgg tctgaaaaac atcccaaaaa taaaaaaatc tttcccctgc
     3001 tagtctccta agtccctttg tagcggatat ggcaccataa tctcatttta tcgaagtgag
     3061 tcatggttta tctgttggcc aaccaaaatt gatacaatct aacgatgcac cctctacggc
     3121 attctagggg agtgcacttg cattcaccca ataaaaaccg gcagttagca aagaacttat
     3181 gtagtcgtat gttcttactg ttaacataca agaggtctcc tttccacaat tgcttagcag
     3241 taaacgggaa tttacaaagc taatctcttc cattttctta tctttgttct aaaagtccca
     3301 aatggagcaa ctgtatttac atgaataaat ttatatactt cccatacagc cgttttggtt
     3361 gcctcgtctg cccattttct tttgtgtgca gtgaatagat cttcatcact cattgaatca
     3421 atcatcatgt taatatcatc aatattacga ttgagaattt cctctaattc tttcaatggt
     3481 agatgtgcat aggtatttgt aaaccactta tataactctc ctaactgatt ccatttaaat
     3541 tgatcagaag gcgttttaac agataatcca tttctttcat cattttccca ttttagaatt
     3601 aaattcgtcc aaccaacttg atatgctaaa ttttcagcag gtgttctatc cacttcctca
     3661 actcttttat ccttcaattc ttcaggaata gtttcaaatt ctaaaatata tttagtataa
     3721 gtcctattta tttctttttt taattcttct ttatttttat atgttctcat tatattacct
     3781 ccataaattc ctaattgtct cctttaattc atctataatt tcatccacga tattttaatc
     3841 cgttctacaa tgcatatctg atatgcattg actgtctaac tcatatgtga atgatttata
     3901 aaatatccca acgtctcatc taaagaaatg ttgatcgtca gcaaggagct aaaaaatacg
     3961 aatatatttc ttttgtagta aaatcggact ggtttgcata tcattttctt cgtaaatatc
     4021 aaaatcatgt taactactat caattactaa taaaaactac cactttaagc gttaagaact
     4081 gttaagttta gttaagtgag gatagttcac aaaatccatt gttaaacttc acgaaagtgg
     4141 taaaactcaa aatgctttat ataggattac agcatcgctc tttcttcgat tgcgcgttgg
     4201 gtaaaaaata ttatgaagta aaaattgacg atgacacgtt ccttacggct catcagatga
     4261 aacagctaca aaaacgaaat gatcaactcg aagaaaattt aattctaaaa aagcgattgc
     4321 catattcacg cctcgctcga cgaactatta aaggtcaaga tttacctgaa gatcatcctt
     4381 tttaggaaat tgaagctttt tatgttgcac aatgcattta taatactaca atgatgtttt
     4441 caccagatat tcttctaggg tgcggtgtaa tgaaacagat acacttgcaa aagaaaattc
     4501 accgcgaatt tactaaaatt atgaataatt atgttaatct tccgaaattt ttttccaaaa
     4561 ttaagggatc aagctggtgt tatagattgt ttaaggttgg caaaaaaata acttcttttt
     4621 aaacaattat agaaaaccga tactctgtta agttggttga cacggtgtcg atttttatga
     4681 ctttcttatt ctgaaacttc actaacggac agtacctaaa aagtctatag tatggacgtt
     4741 tacgagtact attttatcaa aaatgctgct catttcaact tatatgtttt agtgaactac
     4801 ccccactta
//
LOCUS       DAFEPQ010000113         4791 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.119, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000113
VERSION     DAFEPQ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.119"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(75..1073)
                     /locus_tag="LP543_002591"
     CDS             complement(75..1073)
                     /locus_tag="LP543_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBL2322714.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1148..1444)
                     /locus_tag="LP543_002592"
     CDS             complement(1148..1444)
                     /locus_tag="LP543_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBL2322715.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1437..1730)
                     /locus_tag="LP543_002593"
     CDS             complement(1437..1730)
                     /locus_tag="LP543_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322716.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1876..2427)
                     /locus_tag="LP543_002594"
     CDS             complement(1876..2427)
                     /locus_tag="LP543_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HBL2322717.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2601..2900)
                     /locus_tag="LP543_002595"
     CDS             complement(2601..2900)
                     /locus_tag="LP543_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HBL2322718.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2927..3193)
                     /locus_tag="LP543_002596"
     CDS             complement(2927..3193)
                     /locus_tag="LP543_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBL2322719.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            3540..4223
                     /locus_tag="LP543_002597"
     CDS             3540..4223
                     /locus_tag="LP543_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322720.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
     gene            complement(4260..4757)
                     /locus_tag="LP543_002598"
     CDS             complement(4260..4757)
                     /locus_tag="LP543_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2322721.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT         1428 a          912 c          696 g         1755 t
ORIGIN      
        1 taaaaaaact gattctaaga aaattaaata atttttctca aaatcagttt ttctttgata
       61 aataatattt tcaattattt aatcatttga aaattgttag tcattccaat acgttgagaa
      121 attcttccat ctgtataaac acgaaattgt tttccaccag ccatggtcgt gtttccatca
      181 tcatccgtta caatattaat tgaatagtag cttccatttt catcttgcat tagatctttc
      241 atatattcat ttgtgctaat taaattagta tttgatacat ttttatcagg agaatcatac
      301 aaccataaag ctttattatt tagcacagat tctttagcag cttcccaact tgaaattcct
      361 gaactacttg tagattgtgt cgttgtagtt gttgcttgtt gagtagattc ttctactact
      421 ggtgtttgag gagtttgtcc attcacaata tcactgaaac cttgttttaa ctgttgattt
      481 tgagtttcgt taaagtacag ataattattt tcattacctt gattttgttg cgttaaataa
      541 actttaggtt gtccattttg aaaaccgaaa aagtaaacat gctgccctaa atattctcca
      601 gtttctacat cagaataaac tgctactaaa ttgaaatctg catgaccagt tccatcttct
      661 gaccattgta cggtaactgg tgcttcattg attgccattt tccaatcgcc attaatcacg
      721 gcttgtggta aaggagtgcc atacagactt acattattct gaagattgta gcttttatat
      781 tcttgaccta atgtctttcc ccattgtgtg acaaaagttt ctaatttgga agctttattc
      841 gtatcccaca aagtattctt aacctcttca gaagaagatt cagtgattga acttgcactt
      901 gtttccacag attgcttcat ttctgttgta gtttgttcag ttgattgcgt tttacttttt
      961 ttagtctcaa ttgttgtatc agtttctata gtcgctttag tcctcgtatt ctcagatgta
     1021 caaccagtga ataaaattcc cattcctatt agtaataaca gccatttttt cattatattt
     1081 ccccctttca atttctccct cacgtactta ttctatcaga caattaattg atctaaatgg
     1141 taatctttta atcgattctt tcccaataat gtcgatagcc cgtatggcag cggtctcttc
     1201 cttgaggact ctcagatgga ttaaaactag tagaaatttc ccttccacat cctctacagc
     1261 gataagttct cattattttt gggctattag tattattttt cttagttaga gctatgagtc
     1321 caactatgat taccccaact gtcttaatta cttttccttt atctatattc ggatgcgcaa
     1381 gcatatattt atataaccaa tatccaccgt aaattaaacc acctaataca atcagcttag
     1441 ccatgtactc gtctcctttc tttttgttta attacttttt gaatccatct tgcagagaaa
     1501 ccataaactt ctgtcaattg acggatatct acagtttttt cctgttctat catgttttcg
     1561 attctttttc taactaactc acgatcgtac attttcattg gaaaagaaat ctgttgccct
     1621 cgaaaagcaa gataaaattg atagagcaat tctgaatcca gttccatttc ctcaaaaaag
     1681 tcattcatac ttttataaga gttactcaaa caagtttcat caatctccat ctacacaccc
     1741 cctgttattt tttacataat gaaaatagca tattactggg tacattgata attcatataa
     1801 catgtatcat cacatgtatt tttaaagtca aaaagcatgt aaaactactt ataagtaaca
     1861 tgagaaaata aaatgttatt tttcttgaaa taataattga ctatctgcca attgccattc
     1921 attacgaaca tgaatactta gtgggtggga taacttacct tctgcattta tcaatacttt
     1981 ggtcatcttc ttttgtttcg cactcaattg atcgtaaata gtctttgctt gttcataagc
     2041 cacttgactc gtcgctgtaa tcgaaccaca agcaaaaata atggtattga ttttcttttc
     2101 tgtaaaaaca gtcgttaata tttccaaaga ttgttcatca tttcctcgaa gaaatacccg
     2161 aggacttttc gcttttgtgt acgaaaaaag attcacagca ataaaaccag taaatcctaa
     2221 ttgtcggata ttcttttcga ttaataacat actcaaatca ttcgtaaatg gttctgtatc
     2281 tgttggacga atagtaatga ctactgctaa tttatcttcc gcttcttttc ctttgatcgc
     2341 ccattgtcgt ttgaaaacat agcgatgggt atgatgagga tctcgccaaa cttctgtttg
     2401 tgtggtcact aattcttttt caatcatctc tttttctcct atgctatttt ttgttcaagc
     2461 acttcaatta ccattgccgt tataagtgtc aatactagtt gaaaatattt catattcttt
     2521 ctcctttttt cttcagaatt tgatgaatcg cggtaaggat atcttctaaa catttcgcaa
     2581 tcattctcaa ataatcgatc ttattcatcc gttcctccaa tctgactcca aaccaatcgt
     2641 ccttctacca gtaacccacg aacaaattct cgctggctgg taatatctgt aatgaaataa
     2701 actacatagt cttttccatc ttctggtgcg caataaatat aaagatgtgt catcactaaa
     2761 cgtggtagat gaacatcttc aggaagcata cccatttcaa tttcctcttt ttccatcgta
     2821 acggattcat agccccactc ttcagaagca gtgaccagct gtgcacactt ttccagtact
     2881 tcgatagccg tttctaacaa gtcctttcgt tcctttcttt tagatactag tattcttctg
     2941 gtaataacat cgtttgatac tcacccgaat caatggccca tattttttga gacactgatt
     3001 tcaaacgaag cttaggtgaa atcagatact cttttctttc ttccggttct tcttgacgat
     3061 gaaccaactt ttgatttcct ttttccgtag aaaattcaaa aatttgaaga tagtctaaag
     3121 gaaattgctt ttgaacgttt tgatcgatta gattccaaca taaagcttgt aattccaccg
     3181 gcaaactgtc cattactccc tttgtgacat aacgttatcc tttattttga aacatttctt
     3241 ttccctcctt tgccataaaa atagcccaat cttctattaa agaagattgg gcttcatgga
     3301 tataatatat atttgtaaat aaagttaatt cagttataat tatcaaaact acacaattat
     3361 taaagttttt catatctctt tgtttagtaa gtttttctaa atcttaaccc gtggtgctag
     3421 ttaatttttt cctagaggaa aatgtctaaa catgttggta aatcaacaaa aaaacgcata
     3481 tagtagtctc aaaactccca agaaatgagg actatttaac aaactacgat ggttacgtcg
     3541 tgacaactga acattttgac ttagccaatc ctgttactaa agtcgatgat atccccgatt
     3601 atgaaatgta cagtcaaaca atcgatagct taaataagcg ttttggcaat cgtgttttaa
     3661 aaggcatcaa aatcggttac atcgcttctg aaaaggatcg gatcatcgac tatttagccg
     3721 ataaagatta cgatttaaaa ttactcagtg tgcatcataa tgggcaattt gattatttag
     3781 atgacgaagt caaagatatg gatcctgcta ttgtgattcc tcagtatttt gcacaacttt
     3841 cagaagcact agttgttata gaagcagatg tctttgctca ttttgactat ggttttcggg
     3901 tatttggtct atccgttgct gaatttaaac aatatgaagc gcagttttta ccgattctag
     3961 atcaagtaat caaaaataaa ttggcattcg aattaaatgc taagagcgct tacttgtatg
     4021 acaatttagc gttatatgaa tatgtcatcg atttatatct ttcacgtggt ggtaccttgt
     4081 ttagtgttgg atcagatggg cattaccttg aacatttccg tttccatttc gatgatcttt
     4141 ttgctctgtt aaaagcaaat ggtgtgacgg aattagcgat ttatcaaaaa ggaaagcgaa
     4201 ttatggttcc attaccagtc taaaaaaacg tcctcagcat caaaagtgag gacgtttttt
     4261 tagatttctt tttctaaaat cggacaaata cgatcttgtt ttcctaaagg aaaatgtttg
     4321 atcccgattt ttttaaatcc atgacgttca taaaaacgaa tacctgcatg atttttttca
     4381 taaacagtca aacgtaaata acgtttttgt ttcgttttag cataacgatt cgcttcatcg
     4441 agtaaccgtt gccctaaccc tttgttttga taatctttga gcagatagag ccgttgaatc
     4501 tctatagcat ctttttcatc attgactttc atataaccaa tgagtatttg atgatcctta
     4561 ataccaataa atttactggt tgactgtgcc aattctactg ccaatatttc agttgtcaat
     4621 tgtgtctgta aatatccttg catatcaagt ggatctgcgc catcgataaa cgattcataa
     4681 aaactgacaa cacttacaac ttgtaaagca tctagatcat cataatctaa atacgctagt
     4741 gtgatttgat tgttcatcta actcctcctt ttgacatagt atactataaa t
//
LOCUS       DAFEPQ010000114         4786 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.120, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000114
VERSION     DAFEPQ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.120"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            546..1109
                     /locus_tag="LP543_002599"
     CDS             546..1109
                     /locus_tag="LP543_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2322722.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1388..1675)
                     /locus_tag="LP543_002600"
     CDS             complement(1388..1675)
                     /locus_tag="LP543_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL2322723.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1665..1994)
                     /locus_tag="LP543_002601"
     CDS             complement(1665..1994)
                     /locus_tag="LP543_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL2322724.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2299..2628
                     /locus_tag="LP543_002602"
     CDS             2299..2628
                     /locus_tag="LP543_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2322725.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2915..3400)
                     /gene="vanZ"
                     /locus_tag="LP543_002603"
     CDS             complement(2915..3400)
                     /gene="vanZ"
                     /locus_tag="LP543_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBL2322726.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3553..4464)
                     /locus_tag="LP543_002604"
     CDS             complement(3553..4464)
                     /locus_tag="LP543_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HBL2322727.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         1565 a          824 c          723 g         1674 t
ORIGIN      
        1 agttaaaact tttttagcag attttaaagt ttcgtcaaaa ctaatatatt ctggtagctg
       61 agcattcaaa tagctttcag acttcagaag aaaattacga gcatttttat agtccatctc
      121 taataaagtt attctttttt tatccaatac attcagctca attccttata atattctatt
      181 ccttagtatc tcaacattat ttactttata aaacgaataa attttgtagt tctaattcaa
      241 atattgcatg ttatatctaa tatgggagca ttttataatt ttcattgtga taaactcgtt
      301 attaacttta ggatacacta gttaggatta aattaggtat ctagaaattt acttaagatt
      361 tttttgttta tttcgtatac acactcgtat caaaaacaat cgattatgag acaaaatgaa
      421 atgacctctt ttattaaata aaagaggtca tattttgtat cataacttgt tgcattaact
      481 atgtatctat aactcccttt tatgatacaa tcaagccaga attaaagtac gaaaggaaga
      541 aaagcatgag tggatacatt tatggttatg cccgagtgag tacacgttca caagaactaa
      601 atcgtcaatt agacttactc aatgaacaaa attgtaatga aatcctaact gagaaaatga
      661 cgggaaccaa agcggttcgt ccccaactaa atcgtttaaa agataaaatt cgtccgggag
      721 atacggtaat tgtagaaagt ttttctcgat tgggtcgtag tacaaaggat ttgattgacc
      781 ttgttaccta ttttgaagaa caccatgtta agctggtgag cttgaaagaa aacttcgata
      841 ccagcacgcc tcacggacgc ttaatgatga ctgtcttcca agcatttagt cagttcgaac
      901 gtgatttaat tgttgaacgt accaaagaag gtttgaaaag tgcacgtgcg agaggtcgta
      961 aaggtggccg tccacgagtg aaccagaaag atgttgatcg agcagtaaaa ttgtataaaa
     1021 gtcaggttta tagcgtaaaa gaaattacgg aaatgactgg tattagcaag gcaacactct
     1081 accgctactt aaaagacaat agggaataat ctattttaat ctttaggaat ctataatatt
     1141 tatgggagaa ttagagtgat ttttttatta ttactaaaaa acaatataaa ataacgcatt
     1201 ttaagccctc tttgtgcata aggatgaagc ttttaatttg aaagaggctt aaaatgcgtt
     1261 ttaatttttt ctatttttca taaatatata ggttagtggt ttaaaattat agggtattac
     1321 aactctcagc tgttaaaaat tatcttttaa agtgtcgtct atcctctcaa gttgaaacat
     1381 tgggttctta cctaatcttt tttaattgtt cataaaaatt ttcatgcgtt ccacatagta
     1441 agataggtat cactgcatta tctttaattt catatgcaac gcgatatgtc gtacctgcat
     1501 acttgaatcc aatagcccaa attcctgata aatcacccgt tttttgttct cccgaatgag
     1561 gacgaactgc tatctcatca taaattatcg ttaaaaattg ttcttttaat gttttctctt
     1621 ttaatttttt tagatacttt attacgtgtt tagaaggtag aatctcataa gccaatttct
     1681 gctgccaact cctcttttgt catcggttta gaatttgcaa cagtatcttc agcaatagat
     1741 ttaaatgcag aagttaacgc attttttcta tttctaaatt cttttaagat ctcttgtttg
     1801 ctataacctt ctgaaagaat atcagccaaa atatcttcac taaaatcaaa aaattctttt
     1861 ttaggctcta caaactcata aaggatacca ttctctacaa gcttagcttc tacttcaaca
     1921 ccattgggca catcgaattc tttagggacc gttaacataa tagaatttcc ttgttttctt
     1981 gtttttgtaa tcatattatt tcctccatta ttttacattt cgtaattact tagtaattac
     2041 attgtacgct atttttagta gaaatacaaa ataataaaac tcctttagaa caattttcaa
     2101 aggagtttta tttgattagg aaattatgta gaataataat atgtttttaa cttggaatca
     2161 aaaacgcact tgcaagtaag tttacttcaa tacatgaaat tgcttataat agtgtattgg
     2221 aattaaggta gattgacaaa agagtgatca aataggtata cttcattcaa ataatcgttt
     2281 gaatgtgagg tgagatgaat gaaaaaagtt tgccaagtga ctgtcgtaca taaagaaaaa
     2341 gtaaatggaa taaaaaataa attggatcaa aaagattttt caaacttact tgtgttaggg
     2401 aaatgtttca gcgatttttc tagaattaaa attttctatg ctttagaaac ctataaggaa
     2461 atgtgcgtct gtgatttagc agaaatactt actgctagtg tcgctacaac gtcacaccat
     2521 ttacgttttt taaaaaaaca tggaatggca aaatcacgtc aagatggaaa agtagtttat
     2581 tactctttag cgaatgaaga catcctttcc gctattcgtg ttttttaaag ttatcagaaa
     2641 atctatctat gaaatcttag tttgggggta ccgtcaggaa aatgcggatt tacaacgtta
     2701 aggaaattta ttaaagagac caattcctca gcactaaaat tgataattgg gctttctttg
     2761 ttactatagt gttaggtaag ctttttactt tatcaccggt tttcccctca cttcacacct
     2821 acgggcgagt ttccccacat cccgttttcc atctaatcca attcattcaa tatctttatg
     2881 tgtatgaaag atcagattgc ttgatttaga catcttacac gtaatttatt cttaaatggg
     2941 tacggtaaac gagcaataat acgagcaaaa gtatacctac aaaaataata actctgtcta
     3001 attttttttg attcatatgc ttattgctta aaccatataa tttcagtcca agaaagcctc
     3061 caacagtatt tgtaattaca tctgttatgt ctgtcgctcc aatagcgaag ataaattgaa
     3121 ttatttcaaa agtaagactt aaaaccagta caaaagcaaa cttaggtaaa aatccgattt
     3181 ctttaaaatt gacattcaaa agcaagccaa atggaataaa gattataaca ttatctatca
     3241 tctctctgaa attcccagta gcagtaaatg gagtcaagtt aagacttctt tgatgataat
     3301 taaatactga taaaatattg tattgtaatt tgaataacac taaccagatt agtgtcacta
     3361 aatataaagc tagcaatcct ctagataata tttttcccaa tataatttcc tctaattctt
     3421 ttgtttaagt tgtatcatta gtatagcaat gtcctagtta acgtgggttt taaattaaaa
     3481 agctaacgtt accccaaaag tgctagtaca ataatgctgt tcattaattt cgttggtttc
     3541 attccgccat ccttacctcc ttgaattagt atgtgttgat ccgggaaaca ctgtcacaat
     3601 tacaccgtct atattgtttc ctgatatctc ataacgaaga ttagtcggca catgaatggt
     3661 ggtattttta gtaacaggat aataaaagat ctcatatttt tccccattta cactaacaga
     3721 aatggttttt tcttctttta ggtaatccat atattcctcg agaacgaaat tcttttcttt
     3781 cataatcgca ctatgtggta aaccaacata gcgaatatgc catggttcat attgaattcc
     3841 tgttaactct gttttgtcct ctggataacg taaaatgaac ccgtatttcc aagcattttc
     3901 ttctatccac tttccttcag gggctcgttc cattttcgtc aagcttgatc ctacatctag
     3961 tgataaacct gaattatgct cactataacc tgctggtaag gcatactcag cccccatttc
     4021 ttggtaaagc acactttgct catcaaagtc tcgatagcca ctattaataa taaaatgact
     4081 aacgccaccc tttacagcat cattgaccat ctctgaaaat ttttgtgcta tttcttttga
     4141 catataaata ttactatcaa gcaacccgta tccatttatt aattcgtcat gtttagataa
     4201 attcacgata tctgacttca cactttcttg gcgaacagga tatttactat tgattaatag
     4261 cagatttcct tgataaacct gttcttctgt aattgttttc tcttgggtat tttcagaagt
     4321 cccactattt tctaaatgtt ctttgggatt ttgatcataa ttttgaaatt cgactttttc
     4381 ctgagaaaac agtgcttcat taacgtagtc ataacctaag tatattaaga ataacaataa
     4441 caataaaaaa aacaacttct tcattttcag tctcctcctt aactatatag ttggaagtat
     4501 gaaatatttc tagaatcttc cccttaaacg gcacaatctc gtttgagatt gtgccgtttt
     4561 gccccgtaaa ttacagtcaa ttatttcgcc ctactggatt tactattatc accaatgtag
     4621 atgaattttc ataagtgata cccatgctga atttaacgag gctgaactgc ctgttgacaa
     4681 tgggcggtgt gaaataccac ccctctatgc gatcagtctt attatcaggc tatcttgccg
     4741 cagcgcacgc agataatgac cgcaatatcg ctctgcctta catctt
//
LOCUS       DAFEPQ010000115         4726 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.121, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000115
VERSION     DAFEPQ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.121"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            130..1275
                     /gene="tgt"
                     /locus_tag="LP543_002605"
     CDS             130..1275
                     /gene="tgt"
                     /locus_tag="LP543_002605"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBL2322728.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1372..1752
                     /gene="yajC"
                     /locus_tag="LP543_002606"
     CDS             1372..1752
                     /gene="yajC"
                     /locus_tag="LP543_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBL2322729.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKKEK"
     gene            2057..4654
                     /gene="adhE"
                     /locus_tag="LP543_002607"
                     /gene_synonym="adhC"
     CDS             2057..4654
                     /gene="adhE"
                     /locus_tag="LP543_002607"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBL2322730.1"
                     /translation="MAKVMEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     AQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         1655 a          834 c         1010 g         1227 t
ORIGIN      
        1 ataaaaaaag gataaaaaaa agaatattaa ataaaataga aggaagccga gggaacttgc
       61 aaaaagcact tgaaacgatt atgatggtgg gagttgtcaa gatattttat atagaaagta
      121 gggaaatgaa tgacgcaacc tgccattcgc tatcgtttaa tcaagaaaga aaaacatact
      181 ggcgcgcgtt taggggaatt gatcacgcca cacggaactt tccccacacc aatgttcatg
      241 ccagtaggaa cattagctac agtaaaaaca atgtccccag aagagttgaa agaaatgggt
      301 gctggcgtta ttttaagtaa tacatatcat ttatggctac gccctggtga agacttagta
      361 gaagaagctg gcggactgca taaatttatg aactgggatc aaccgatttt aacagattca
      421 ggcggctttc aagttttttc attaagtgat atgcggaata tcgaagaaga aggcgtgcat
      481 ttcaaaaatc acttgaacgg ttcgcgtatg tttttatcac cagaaaaagc aatcaacatc
      541 caaaataaat tgggctctga tatcatgatg agttttgacg aatgtccacc attcgatgaa
      601 agttacgact acgtgaaaaa atcagtagaa cgtacgtctc gatgggcaga acgaggattg
      661 aaagctcatg ctaacccaga ccgtcaagga ttatttggaa tcatccaagg agcaggattt
      721 gaagatcttc gtcgtcaaag cgcgaaagat ctagtcggga tggatttccc tggttactca
      781 atcggcgggt tatctgtcgg ggaacctaaa tcagaaatga atcgtgtatt agaattcact
      841 acaccgttga tccccgaaaa caaaccacgt tacttgatgg gtgtcggtgc tgctgattca
      901 ttgatcgatg gtgttatccg tggagtagac atgtttgact gtgtcttgcc tactcgaatc
      961 gctcgaaatg gcacatgtat gacttctcaa ggtcgtctag tagtgaaaaa tgcgaagtat
     1021 gctcatgatt tccgtccaat tgatgagaaa tgtgattgtt atacatgtaa aaactataca
     1081 cgtgcctata tccgccattt gatcaaatgt gatgaaacat ttgggattcg tttgacttct
     1141 taccacaatt tatacttctt gttgaatcta atgaaacaag tacgtcaagc aatcatggat
     1201 gataatttgc ttgaatttag agaagccttt tttgaagaat acgggttcaa taaagaaaac
     1261 gcaaaaagct tctaaaggaa aatcttttaa aaattaagta aaaaactagt gttagcagca
     1321 tttttctgct aaagtgtttt ttgtagatac agaattagtg aggtgaaaaa aatgggaaca
     1381 ttaccaatgc ttgtgatgtt tatcgcgcta ttagcaatgt ggttttttat gtcacgttcg
     1441 caaaagaaac aacagcaaga acgtcaaaac ttgttagata gcatgaaacc aggtgattca
     1501 gtcgtaacga tcggcggatt acatggagtt gtttcagaaa tcgacaatga caaacggaca
     1561 gttgttttag actgcgaagg gatcttcctt gagtacgatc gtgcagcaat caaaacggtc
     1621 aaaccaggga ctgtcgtaac aaatgatgca acaactactg ttgaaacaac agaagaacca
     1681 gctgagacag ttgaagtaaa aactgaaaca acagaaacgc tggaagagac agaagagaag
     1741 aaagaaaaat aagtatcaaa gcttatttaa agacgaggta gggcagaaag atgtcctacc
     1801 tcatttttta ttcaaaaaag aaagagataa gagtatagga tatattgctc aatattgaat
     1861 aatcgggtgt gtcatataaa taataataaa taactttata tatttttcta tacaaacgcc
     1921 tataaataaa gcaactttat atttaaaaat aactgtgaaa aaaatcacaa aaaagtgttt
     1981 acatagaaaa ataaatattg tatgatatac atgtgaatga tgtaacaaat aaccatgacc
     2041 cattaggagg ataatgatgg ctaaagtaat ggaaaaagaa aaaacgaaga caattgacgt
     2101 acaagcaatg attgatgatt tggcagaaaa agcaaatgca gcattgaaag aaatggaaag
     2161 ctttgatcaa gaaaaagtgg accatatcgt gcatgaaatg gcaatggcag ctttagatca
     2221 acatatgcca ttagcgaaga tggctgtaga ggaaacaggc cgcggtgttt acgaagataa
     2281 agcaatcaaa aatatgtatg cgtccgaata tatttggcat aatatcaaac atgataaaac
     2341 agttggtgta atcaatgaag atgcccaaaa aggattgatt gaaattgctg aaccagtcgg
     2401 tgtggtttgc ggagtaactc caacaacgaa tccgacttcc acaacgattt tcaaatcaat
     2461 gatcgcgttg aaaacaagaa atccaatcgt ttttgctttt catccaagtg cccaaaaatc
     2521 ttctgcagaa gcagcacgag tagtacgtga tgctgctatc gctgccggag ctcctgaaaa
     2581 ttgtatccaa tggatcgaac atccttcgat cgaagcgact tctatgctaa tgaaccatcc
     2641 aggtatcgcg atcgtcttag cgacaggtgg agctggaatg gtcaagtctg cttactctac
     2701 aggaaaacca gctttaggtg taggtccagg taacgtacca gcttatattg aaaaaacagc
     2761 aaaaattaaa cgtgcagtga acgatttgat cgtttcgaaa acatttgata atggaatgat
     2821 ttgtgcttct gaacaagctg tgatcgtgga taaagaaatc tatgcagcag taaaagcaga
     2881 attccaagca caccaagttt atatcgtaaa accagatgaa ttgcaaaaat tagaagatgc
     2941 agtgatgaac gaaggaaaat atgcggtcaa tccatcaatc gttggacatt ctgcgatgga
     3001 aatcgcaaaa ttggcaggaa tcagtgtacc aaaaggaacg aaaatgctga ttgctgaatt
     3061 ggaaggcgta ggcccagatt atccgctatc aagagaaaaa ctttctccag ttcttgccat
     3121 gatcaaagct aacaacacgg accatgcgtt tgatttgtgt gaaggtatgt tgaatcttgg
     3181 aggattagga catacagcag ttatccattc agaaaacgaa gagttgcatg tcaaattcgg
     3241 tcttcgaatg aaagcttgtc gtattttagt gaatacacca tctgcagaag gcggcatcgg
     3301 tgatatctat aacgaaatga ttccgtcatt gactttggga tgcggttctt atggtaagaa
     3361 ctctgtttct cgtaacgtct ctgctgtaaa cttaatcaat gtcaaaactg tagcgaaacg
     3421 gagaaataac atgcaatggt tcaaattacc gccaaaaatc ttctttgaaa agaactctct
     3481 attgtactta gagaaaatgg aaaacgttga acgtgtcatg atcgtttgtg accctggtat
     3541 ggtccaattc ggttactgcg atacagttcg tgaagtattg gcacgacgta aaaatgatgt
     3601 gaagatcgaa gtcttctcag atgttgaacc gaacccatcg acaaataccg tatacgcagg
     3661 aacgaaactg atggcagact tcaagccaga tacagtaatc gctcttggag gtggttctgc
     3721 aatggacgca gctaagggaa tgtggatgtt ctatgaacac ccagacacat ctttctttgg
     3781 tgcaaaacaa aaattcttgg atatccgtaa acggacatat aagatcgaca aaccagaaaa
     3841 aacacaattc gtatgtatcc cgacaacatc agggactggt tctgaagtaa cgccatttgc
     3901 ggtcatcact gacagtgaaa cacatgtgaa atatccgtta gcagattatg cattgacgcc
     3961 agatgtcgca atcgtcgatc ctcaattcgt catgtcagtt ccagcatctg tcacagcaga
     4021 taccgggatg gatgtgttaa cacatgcaat cgaatcctat gtttcagtca tggcttcaga
     4081 ttatacacgt ggattgagcc tacaagcaat caagttagtc tttgactatt tagaaaaatc
     4141 agtaaaaaca cctgatatgg aatcacgtga aaaaatgcac aacgcttcta caatggctgg
     4201 tatggcgttt gctaacgcat tcctaggtat ttgtcactca gtcgcacata aaatcggcgg
     4261 ggaatacggg atccctcatg gacggacaaa tgcgattctt ttaccgcata ttatccgcta
     4321 taatgcaaaa gatccacaaa aacatgcgat gttccctaaa tatgactatt tccgtgcaga
     4381 tacagattac gcagatattg caaaattctt aggtctgaaa ggcgaaacaa cagaggaatt
     4441 agtcgaagct ttagcaacgg ctgtttatga attaggaaaa tctgtcggca tcaacatgag
     4501 tctaaaagct caaggagtca cacaagaaac actagataca accgtagacc gtatggctga
     4561 attagcttac gaagaccaat gtacgacagc taatccgaaa gaaccattga tctctgaatt
     4621 gaaacagatc atcattgatg cttacaacgg ataagcaaaa gaaagcgtag aaaaaagata
     4681 agaaaataga tgaaaaagaa cttggaaaac gttgtttctc caagtt
//
LOCUS       DAFEPQ010000116         4600 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.122, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000116
VERSION     DAFEPQ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.122"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..936
                     /locus_tag="LP543_002608"
     CDS             <1..936
                     /locus_tag="LP543_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL2322731.1"
                     /translation="GRWKETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESL
                     AIDILTFLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADH
                     IESFTDYDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVA
                     MLIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFV
                     TQNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQC
                     LISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            939..3122
                     /locus_tag="LP543_002609"
     CDS             939..3122
                     /locus_tag="LP543_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBL2322732.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3119..4120
                     /locus_tag="LP543_002610"
     CDS             3119..4120
                     /locus_tag="LP543_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBL2322733.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4135..>4600
                     /locus_tag="LP543_002611"
     CDS             4135..>4600
                     /locus_tag="LP543_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL2322734.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLI"
BASE COUNT         1436 a          911 c          989 g         1264 t
ORIGIN      
        1 ggcagatgga aagaaacctt gccagagatt tcccatgaaa tcaatatcgt caatctgact
       61 tctgatgaga aaaacaaagg cttacttgac ccttatgtaa ttatgaaaaa taccaaagat
      121 tctgaatcac tggctattga tattttgaca ttccttacgg ggatttcctc tcgtgatggg
      181 gaacgcttcc caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtgcgaggg
      241 ttgatgaaag tgattgaaga attacgggtt gagaatacgc cactaagtac cagcatagcc
      301 gaccatatcg aaagttttac agactatgac tttgcccatc tgctttttag tgatggttat
      361 gtagagcagt ctatcagcct tgaaaaacaa ctgaacatta tacaggttgc cgacttggta
      421 cttcctgaca aggaaacttc ctttgaggaa tataccacaa tggagttact atcggtagca
      481 atgctgattg tcattagtac ctttgcgttg gactttatcc atacagaccg aagtattttc
      541 aagattgtag acttagacga agcatggagc ttcttacagg tggcacaagg gaaaacacta
      601 tctatgaagc tggttcgtgc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa
      661 aatacagatg acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgccttt
      721 cgttccactg accttaacga gattaaaaaa acccttgcct tttttggtgt agacccagag
      781 gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat
      841 ttatatggtc gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc
      901 tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atattaaaca
      961 gaataaaatc aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca
     1021 cacttgtgat tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct
     1081 tggtagatga tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc
     1141 aactggattt ttatgtggat aatagctggg gctggctacc gtggaactgg tcggacggaa
     1201 ttggaaaaca agtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtc
     1261 tttatgtttc caatgcgacg ggttacttag tacaggaagc ctattcctta gacttcattt
     1321 ccgctacagc agattccatt ggcaagaata tgcagaccct tgcaggggtt acgcctagtg
     1381 gactatcaac agagggtttc tatgttggat tccttttact cttgattttg gttcttgggg
     1441 tttatgttgc ctatacagga ctgataaaga gagaaaccac aaaggcaatt catgccctca
     1501 tgaattttgt gctggttttt atcctatcgg cttcctttat tgcctacgct cccgactaca
     1561 ttaaaaaaat caatgacttt tcatcagaca tcagcaacgc tagtttgtca ctgggtacaa
     1621 aaattgtcat gccccattca gatagtcaag gcaaggacag tgtggacttg atacgtgaca
     1681 gcctattttc catacaggtt cagcaaccgt ggctactgct tcaatataac agttcagaca
     1741 ttgaaagtat tggtattgac cgtgtggaaa gcctgctctc aaccagtcca gattccaaca
     1801 atggggaaga cagagaaaaa attgttgcgg aagaaattga agatagaagc aataccaata
     1861 tgacgattac aaagacgatt aaccgtttag gtacagtctt cttcctattt gtcttcaata
     1921 ttgggatttc catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt
     1981 ttatcatcta tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg
     2041 atggtatgtc aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa
     2101 tcacattgat tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg
     2161 gttatccgtt ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttta
     2221 agctgggcga tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa
     2281 gtcgtatgat gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga
     2341 aacttggacg ctccgtgact gctttagggg ctgggtctgc cattgccagt gctacgggta
     2401 aaaaaggaca gtcgggttcg ggaagttctg cgagtacaca agcagatcac tctcgaccaa
     2461 acgggcagga aaaatcaaca ctaggaaagc gtatcggtca aaccatcggt acagtagctg
     2521 ataccaaaga cagaatggta gatactgctg gtaatttgaa agaacaggtt aaggatttgc
     2581 cgaccaatgc aagatatgca gtatatcaag gaaaatctaa agtgacggat aatgtccatg
     2641 atttaaccag aagtatttct caaaccaaag cggataaagc cagtggacgc aagaaacagc
     2701 aggaacaaag acggaaaacc attgcggagc gtcgctctga aatggaacag gtcaaacaga
     2761 aaaaacagcc agcttcttcc gttcatgaaa gaccagccac taagcaagaa caatctcatg
     2821 atggacagac ttcaaggcaa acaactgtac aggcttccta tagggaatct caacaagcca
     2881 aacaagagcg tccaacagtt aagtccgatt cttcaagttt aaaaacggaa cgtcaaagta
     2941 atacagttca agaaagaacg gttcaaaagc cagtaacttc aactgcacca acagatagag
     3001 cttcacaacg ttcaatcaca aaagaacgtc agtctactgt tcaaagagta ccactacaaa
     3061 atacaaaaag tagaccacca atcaaaaccg ccaccattaa gaaaggcagt aagaaaccat
     3121 gaagctgaaa actttagtga ttggtggttc gggattattc ttgatggtct tctcactgct
     3181 tctgtttgtc gccattttat tttcagatga acaagatggc ggtttttcca acattcacta
     3241 tggcggtgtg gatgtttccg cagaagtgct ggctcataag cctatggtag aaaaagttgc
     3301 caaagaatat ggaattgaag aatacgtcaa tatacttctt gcgattatac aggtggaatc
     3361 gggtggtaca gcagaagatg ttatgcagtc ctcggaatcc ctcggtcttc cacctaattc
     3421 attgagtaca gaagaatcca ttaagcaagg tgtgaagtat ttcagtgagt tattagccag
     3481 tagcgaaagg cttggtgtag atttagaatc ggttatccag tcctacaatt atggtggtgg
     3541 tttcttagga tatgtggcta atcgtgggaa taaatatacc tttgaactgg ctcaaagttt
     3601 ctcaaaagag tattcgggtg acgaaaaagt gtcttacccc aatcccatag ccatacctat
     3661 caatgggggc tggcgataca attacgggaa tatgttttat gtgcaactgg taacgcagta
     3721 tcttgtcaca acagagtttg atgatgatac agtacaagcc atcatggacg aagctctgaa
     3781 atttgaaggc tggaagtatg tctatggcgg agcttccccg actacctctt ttgattgtag
     3841 cggactgaca caatggacgt atggaaaagc tggaatcaac ttaccacgaa cagcacaaca
     3901 gcaatatgat gtaacccggc atatcccatt attggaagca aaagccggcg atttggtttt
     3961 ctttcattct acctataacg ctggctctta tattactcat atcgggattt atttaggcga
     4021 taaccgtatg tttcatgcag gcgaccctat cggttatgcc gacttaacaa gctcttactg
     4081 gcaacagcat ttagtgggag caggacgaat caaacaataa gaaaggacga ttcaatgatg
     4141 aaattcggaa aaaatcagaa tacagaaaaa cagacaccaa aggaaaagaa acctcgtgtc
     4201 tataaggtca atcctcgtaa aaaggttgtg attgtcctat gggtactttt aggacttagt
     4261 ttcagctttg cgatattcaa gcactttaca gccattgata cccataccat tcatgaaacc
     4321 accatcatag aaaaggaata tgtcgatact catcatgtgg aaaattttgt agagaacttt
     4381 gcgaaagtct actattcatg ggaactaaac gacaagtcca ttgataatcg catagaaaac
     4441 ctgaaagcat atctgacaga ggaacttcaa gctctgaatg tcgatacagt tcgtaaagat
     4501 attcctgtat cgtcttctgt aaaaggattt cagatatgga cggtagaagc agatggcgac
     4561 aatcaatttg atgtaaccta cagtgtagac caactcatta
//
LOCUS       DAFEPQ010000117         4581 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.123, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000117
VERSION     DAFEPQ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.123"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(3..203)
                     /locus_tag="LP543_002612"
     CDS             complement(3..203)
                     /locus_tag="LP543_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL2322735.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(337..945)
                     /locus_tag="LP543_002613"
     CDS             complement(337..945)
                     /locus_tag="LP543_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBL2322736.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(1030..1500)
                     /locus_tag="LP543_002614"
     CDS             complement(1030..1500)
                     /locus_tag="LP543_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBL2322737.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(1548..2462)
                     /locus_tag="LP543_002615"
     CDS             complement(1548..2462)
                     /locus_tag="LP543_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBL2322738.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            2608..3411
                     /locus_tag="LP543_002616"
     CDS             2608..3411
                     /locus_tag="LP543_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBL2322739.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            3589..4536
                     /locus_tag="LP543_002617"
     CDS             3589..4536
                     /locus_tag="LP543_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBL2322740.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         1469 a          901 c          712 g         1499 t
ORIGIN      
        1 tattataatt tagtcacttc agcagcttga ggtccgcgtg caccttcaac aatcgtgaat
       61 tctacttctt gaccttcttc taggctcttg aagccgtctc cttgaattgc tgagaaatga
      121 acaaatacgt catctccgcc gtctactgta ataaacccaa atcctttttc attgttgaac
      181 catttaaccg ttccgtgttc cataaatgta tagcctccaa aaaagttctt gcaagtaacc
      241 ttgcaagaac attataccca atttagaaaa cgctagcaag ctttcgctct tttattttcc
      301 aaattatttt taagactgat atgacggagg aaatggttat tttgctgctt cgaaacgttt
      361 gtttacttca tcccaattaa taacattcca gaatgctgca atataatctg gtcggacatt
      421 tttatatttc aaataataag catgttccca aacgtccaaa cctagaattg gtgtctttcc
      481 ttccatcaat ggagaatctt gatttgcagt agaggtaata gctaattttc cattttccat
      541 tacaagccaa gcccatccag aaccaaatcg tccagcggct gcttttttga attcttcttt
      601 aaaagaagaa aaatcaccaa aagcttcatt aatcgcttct tttatttctc ctgtaggttc
      661 gccaccagca tttggtgcca tgatttccca gaaaaatgaa tggttagcat gtccgccacc
      721 attgttacgt acagctgtct tgatatctgt tggaatagcg tccatatcag ataataattc
      781 ttctattgtt ttttcgccta attctgggta tttctcaatt gctgcattta aattcgtcac
      841 ataagtattg tgatgcttat catgatgcag atgcatcgtt tcttcgtcaa tataaggttc
      901 caatgcgtca tatgcataag gtagatctgg taatgtgtaa gtcataaaaa aattcctcct
      961 caatgttttc tttataatgt ctacattaca aagagtaaca aagagaagga aattcttcaa
     1021 ttgttatgcc tagtcgtctt tttcactttg acctacttct tcttcaaaaa cctcagtttc
     1081 tttaaaaata aacaacttgc tgaaaatata gttcgctagg ataacaataa tttgagtgat
     1141 gattttcgcc acatactcat tgctgttcat aacattgatc aacaatacca ttgcccccat
     1201 gtccaatccg taagaaagga tacggtagaa gaagaactta ccgcattcaa taagtgcatg
     1261 tgtaaaattc ggtgattttg aatggaatac ccaaagtttg ttcgttacaa aagcaaacaa
     1321 tacagaacaa aaccacgaaa tagaattgga taaggccata cttaaatcaa aatattggcg
     1381 tgcgagagca aaactaacaa aatttacaat cgtagccaaa ccaccaaaaa agagatagac
     1441 tagtatctcc caatatcctt ttgcttctaa atatcctttt aactgtcgat actgcttcat
     1501 gagccaacct accttattaa aaattcttat ttatcatact ctatatttca ataactttca
     1561 tctttattta aaaaaattaa ttttttttga tcctttgcct ttgtctctgc ttggatcgtc
     1621 acatgtgtaa taccatattt taattttaat aaagaaatta tacgaggata tatcttttca
     1681 atctcgctta gtagctgatc ttcaaaatca atatgtccgt cgaaaataat tatcttttcg
     1741 ctatactgcc aaatatggac atgatgaaca ttctttaccc catcgattgc ttcaatttct
     1801 tgttccattt tttcatagtc aatagcaggt gcactttgta tcaaaatagc ggcagctttt
     1861 ttgatcacca ttatcgcttc tatcaatata tatatcgaaa tgatcagtgt aataactgga
     1921 tcgatccagt agatatcgaa aaaacggatc aaaattgctc cgataatcac acttaccgaa
     1981 gacagtgtat cacttaacaa atgcaaataa ctagatttta tattcaaact ttcatctgct
     2041 tcttgtcgta ataataatac tgagaacaaa ttcgcgagca atccaataac tgctactgtt
     2101 agcatcaaat gactatttat cttttctggt gaagtgaacc gcttgaatgc ttcaacggtt
     2161 aaaaacaagg ataatatgat caatatgaat gagttgatga aagcactcaa tatttctaaa
     2221 cgctggtagc catacgtacg ttttttattt gcttcttttc gggccatttt ttgtgccccg
     2281 taagaaaaaa taattgccaa tgtatcacta aggttatgga aagcatctga aacaagagac
     2341 aaactccctg acataatccc tccaataaat tctgataaag tgatcaccgc attcaaaacc
     2401 actactccaa atagtttttt tgatgatctc tgtggctgtg taggcactat tccttcctcc
     2461 attttattta tattggatag tctttagaca agaataacat ttttttgata tttcaccaat
     2521 cattcagatg tttccctaaa gcacttgtat ttcacttgat acttcgctag aattgcaagt
     2581 gatgacacta ctagatgagg agtacttatg acaacaaaac aattaattat cagttccttt
     2641 atacgattca aagacctaaa ggagacgaga aatgtcccat tttggaaagc cattgtctat
     2701 cttttagttt tgagtataat tatggctttg ccaatctctt ttcaagtttt tcaagtctta
     2761 aacaacattc aattggatgg gcaaaagatt gctactcgca ttcctgattt tacaatccaa
     2821 aacgggcagc ttcaaaccga agaaaaagaa ggattcattt accaaacaaa ctccattatc
     2881 ttcacatttg atccagaggg aaaacgaaca gaacaggaca tctctactga tttgatgggg
     2941 aattttgtta gtgtcgggat gttaaaaaac aaacttatca tagctcttcc taatacaggc
     3001 acgacttctg ctttgttgaa caataaccaa ttagaacttc cctacaccaa tgaatcttta
     3061 aaaaacttaa caggtaagga gctccgttct tttttaagta aagcatctat accactttgg
     3121 ataaaagtcc tgacatttat attttctatt tatcctagtt ttctgaatct gctgatcact
     3181 ttgctattta ccaacattgc tgcttttctc tatgcacgct ttcgactaac aaaagctact
     3241 tttcttgatt gtttaaaaac attgatctat tcaatttcat tgcctactat aattgcaaca
     3301 atcttgatgg tctttcttcc ttcttttgac acaagcgcct tcattgcttt tgcaggaatc
     3361 tttatttttg ctcaagctgt caaaggatgg gcaaaaatat cgatcaatta agaaataaag
     3421 acaactcgtt atttctactt caatagaccc ttttagaggt tgtgacattt ttttgtcaca
     3481 gccccttttt tatttgaaaa atatttcttt ctcaaaaaac aagcaaggcg cttcaaagtt
     3541 cttcttgtat gcgctacact tagagtaaag aaaggcggga aattaactat gtcagaaaga
     3601 atcatttatg cagatcgtcc atttcgtgat gaatttatcg aagaaatcca tactatagcg
     3661 cctggttata tctttaaaac agaattacaa ccagaggatc tacaaaatgt agagatcagt
     3721 ttgggctgga actcagacta ccaagaaaaa ctattagcct cttctacctt gaaatgggtc
     3781 caatctatct cagctggtgt tgatcagcta ccccttgaga ctttttcaaa gaaacatatt
     3841 ttactttcaa atggtagtgg catccacagt gaatcgatta cggagcatgt cttaggaatc
     3901 atccttgggt attctcgtgg tctttttcaa gcacaaaaag cacagagtga catgaaatgg
     3961 ttaggttcat cggtccatta ccaatctatc caaggaaaaa aaatactaat cattggaact
     4021 ggccatattg gaaaaatgat tgcaaaaaaa tgcaaagcat tagacatgaa ctgctatgga
     4081 atcaatacat ccggtcatcc agcagaagga atggaaaaga ctttttcttt aaatcaacta
     4141 aaggaagttc ttcctgaagt agatttcgta gtcaatatct tacctctaac ggaggaaaca
     4201 actgggctct ttgatcagca tatgtttgaa cactttgatc caaaatctgt attcatcaat
     4261 gttggccgtg gtgcttctgt taagacacaa gatctggtgc aggctttaaa taaccagcaa
     4321 ctttcctttg cagcattaga cgtttttgag gaagagcctc ttccacaaga tcatcctctt
     4381 tggaaaatgg acaatgtact gattacttcc cacattgcag gtttgacacc tgattttcaa
     4441 aagaaattaa tggctatttt tttaaccaat ctgaaaagct actttgccac ccatgagctt
     4501 caaaccaatc aagtaaaatt gtctgcagga tattaatagc tgaaaacaat attttcaaaa
     4561 gaacaaagag aaggtgacaa a
//
LOCUS       DAFEPQ010000118         4506 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.124, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000118
VERSION     DAFEPQ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.124"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(619..2124)
                     /locus_tag="LP543_002618"
     CDS             complement(619..2124)
                     /locus_tag="LP543_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL2322741.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2121..2807)
                     /locus_tag="LP543_002619"
     CDS             complement(2121..2807)
                     /locus_tag="LP543_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2322742.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(3000..4421)
                     /gene="gndA"
                     /locus_tag="LP543_002620"
     CDS             complement(3000..4421)
                     /gene="gndA"
                     /locus_tag="LP543_002620"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBL2322743.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1255 a          932 c          743 g         1576 t
ORIGIN      
        1 gtttgttgtc ttgaattgaa tcaagacgcc ctacacattt ggtttgtctt actttgttca
       61 gttttcaaag gtctaatctt tttgttttaa caactcttta atcttatcag attcagaaga
      121 gaatgtcaac aacttctttg tttatttttt ctaaacgtct ttgccactca ttagcagcga
      181 cttgattatc ataccatctt tattgttatt tgtcaataac tttttcgaag tttttttcga
      241 taaaatcaag ctttattagc tatctcatcc aaacgacttc gttattataa catgacatca
      301 aaagtgcgtc aacactattt tttataattt ttagtgacac cttaaaggaa tcatttagaa
      361 caaataataa tatagcaatc tctttataaa attgcaatag aaaattccct atttttattt
      421 attattttta aacacgaata ttacagcatt ttattgtttt ttcgttcatc taaaagatac
      481 ttacatttaa ttaacggatg ttattctatt taattgttcg ctagtttttt tataatgaca
      541 tccctgaatt tctcacttgc ttgtattttg taccttattc tttgttggat atataaaaaa
      601 acaagtaaag cacatgcttt acttgtcttc tgcctttgcg ataggaataa agattctaaa
      661 aattgtccct tgccctacta cactttctgc taagatacgt cctttatagt tttcaaccag
      721 ttgtttagcg atagacaacc ccagaccatt tccacccttc gttctagctc ttgctttatc
      781 cactcgataa aaacgattga agatcttatt aaggtcttct ttgggtattc cttcgccaaa
      841 gtcctgaatc gcgatctcaa attcattcag agtggaagaa atagagatat ggacttcttt
      901 tcgtgtagtt gaatacttta ctgcgttatc caatatgata atgattaatt gttcaaagtg
      961 attacgataa atttgcaggg tcacttcttt tgtcaaatca tcatctaaag tgatcacata
     1021 gtctggatat aatatcttga aattattata aacttgatag gtgacttctt ttcctttagt
     1081 tgtttcattc gcataatgaa catctacttg ttctgctctt gataagtcta gcatctcttg
     1141 aactaagctt ttcatacggc tgatttcttg catgctggcg cttaatgatt cttctaatat
     1201 ctcaggatca tctttccccc aacgatttag cagactaaga tgcccttcaa taattgcaac
     1261 aggtgtcctt aactcatgag atacgtcttc cacgaattgc tcttgttgtt caatatacaa
     1321 acgcatacgg tcaagcatct cattgaatat ttcagctaaa tctgccaatt catcatttgt
     1381 gtcgatttca ggcatgtgga tatctgattg cggatcttta cgtatagtat ccattgtatc
     1441 tctgagtacc ttgagtggtt ttaagaaata agaagataaa aagaaaccta aaatactact
     1501 taagatcaaa gaaacaactt ctaaaacaat aagagtcaat aatagcttat ttcgaatatc
     1561 atagaaagaa gataattcat agaagctttg cgcatagccg atcttttctc ttgtaccttt
     1621 agaatatata ggctggatag ataaaaagcc agtttttcct tctagtgtaa cgatcgttgg
     1681 aactttacgt gtagtctgaa tcaggtttcg actgttttca tgggttttaa aaatcaagtt
     1741 ctcgtccaaa tcataaacat aaagatccat tgatggctgc cctagctcag ctataaagga
     1801 atcgatcttc attaagtttc cttcgaccag tgaagtatga tctacgacct catctccgtc
     1861 atttgtcgct ttcgtcagat tacgatacgt atttactaat gtcagttctt catccgagtt
     1921 agccaaacga gaagctactt ctgaaatcgt acgctctaca ttgtttcttt cttttgccac
     1981 aatcagattg atagatgatt tataggtaat gacagcgaaa attgtaaaca caacgaatat
     2041 gaagaaagaa ctcgcaaagg cccacttgat tgtcaaggag ggccctttca gttctctttt
     2101 ttccgatttt ttcggcttta tcacgagcgc atcacataac ctgttccgcg tacagtttgg
     2161 atatagcttt cctccccggg aacgtcaatt ttgttgcgaa gataacgaat atatacgtcc
     2221 actacgtttg tttcaacttc tgtttcgtat ccccacactt tatttagtaa aacgtcacgt
     2281 gctaatacca cgttcacgtt ttccattaaa gtaagcagca actcgtattc tctttttgtt
     2341 agttcaatta cttcatttcc gcgacgtacg acacggtttt ctttttcgat agtcaaatcg
     2401 cgataagtaa tagtagtttg tttagccaca tttttatcgc cttcaatatc gattcgacgc
     2461 aacaatgcac gaagacgtgc taataattct tcgatagcaa atggtttgac gatgtagtca
     2521 tctgctccgt ggtcaagtcc agatacacga tcgatcacag aatctcttgc agtcatcata
     2581 atgattggtg tatttttgac ttggcgtaca cgtctgcaga cttctaaacc gttcaattct
     2641 gggagcatca gatccaacaa tatggcatcc cactcattat ttaatgccgc atctaaacca
     2701 gttcggccgt tgtagtggac ttctgcgtta tacccttcat gcttcaactc tagctcgaca
     2761 aatcgtgcca agttcttttc atcttcaatg attagaatat tgctcattta ttattttatc
     2821 ctttctattc tactggcttt ttaaaaactc tatcattata ccgataatat gcttaaaaat
     2881 ctatagtcat tttatcatgg atagcaattt taaaaaagag ctgaagtcta ctcataacaa
     2941 tcgacttcag ctctttcgtc ctctttttat tttctcattt cctaacagcc gaaagactat
     3001 tattcttcat ggtaccatga gtaatggaag attccttctt tgtcgacacg ctcataggta
     3061 tgtgcgccaa agtaatcgcg ttgggcttgg attatatttg caggcagacg ttctgctctg
     3121 tatgaatcga agtaagcgat ggctgatgag aaagtcggta caggtacgcc agcttggaca
     3181 gccaaggcaa cgacgtctct gactgattgc tgatattttt tcgtgatttc aacgaagtaa
     3241 tcatctagca ataaattttc gatctcagga tttttgtcat aggcatccgt aattttttgc
     3301 aagaatcgag ctcggatgat acatccagcg cgccagattt ttgcgatttc accaaatggc
     3361 aagtcccaat caaattcttt tgaagcgaca cgtaattgag caaatccttg ggcataactc
     3421 atgattttac tgaaatacaa cgcttctcgg attttttcga ccaattcttt tttgtctccc
     3481 gtgaactcaa aatcgttcgt acgagacaaa atcttgcttg cttgtacgcg ttcttcttta
     3541 taagcagaaa tatagcgggc aaatacagat tcagtgatca atggaagtgg cacacctaag
     3601 tcaagcgcgc tttggcttgt ccatttacct gttcctttat tcccagctgc atcaaggatc
     3661 acatctacta ctggtttacc tgttccttca tcgtctttac gtgtcagaat gtcagcagtg
     3721 atttctatca aatagctgtc taattcgcct tgattccatt ctttaaagat atcagccatt
     3781 tcttctactg aaaggtttaa aatattcttc attaagtcat aagattcagc aatcagttgc
     3841 atatctccat actcaatacc gttgtgtacc attttcacat aatgtccggc tccatttggg
     3901 ccaatatatg tgacacatgg tgctccgtct tctgcttttg cagaaatttg ctctaagatt
     3961 ggtgcaacta gcgcataggc ttctttttgt ccaccaggca tgattgaagg tccttttaaa
     4021 gctccttctt caccaccgga aacgcctgta ccgataaagt tgatgccaga gttggctaac
     4081 tcttcattac gacgcatcgt atctttaaag aatgtatttc ctccgtcaat caaaatatcg
     4141 cctttatcaa ggtgaggaag taactcttgg attgttgcat ctgttgccgg tcctgctttg
     4201 accattagca agatccgacg tggtttctcg atagattcta caaattcttc gattgtatat
     4261 gttgctttga aatttttatc tgggtgttct tctactacag cggttgtttt agcccctgta
     4321 cgattgaata aagccactga atagccgcgg ctttctatgt tcaaggctag atttttaccc
     4381 ataacggcca taccgacaac gccgatttgt tgttttgaca tatgaactcc tcctacatac
     4441 gggatttctc cctaatctct tacattatag cttaacacgg ctacattgca aactaaagaa
     4501 caacat
//
LOCUS       DAFEPQ010000119         4402 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.126, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000119
VERSION     DAFEPQ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.126"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(99..1619)
                     /locus_tag="LP543_002621"
     CDS             complement(99..1619)
                     /locus_tag="LP543_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBL2322744.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(1862..2047)
                     /locus_tag="LP543_002622"
     CDS             complement(1862..2047)
                     /locus_tag="LP543_002622"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HBL2322745.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(2031..2213)
                     /locus_tag="LP543_002623"
     CDS             complement(2031..2213)
                     /locus_tag="LP543_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322746.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            2454..2672
                     /locus_tag="LP543_002624"
                     /pseudo
     CDS             2454..2672
                     /locus_tag="LP543_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            3167..3766
                     /locus_tag="LP543_002625"
     CDS             3167..3766
                     /locus_tag="LP543_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL2322747.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            3830..>4402
                     /locus_tag="LP543_002626"
     CDS             3830..>4402
                     /locus_tag="LP543_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage abortive infection AbiH family
                     protein"
                     /protein_id="HBL2322748.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT         1403 a          799 c          785 g         1415 t
ORIGIN      
        1 ataattcagt agttttgagc gtaagcgtgt catagaatgg tacctaaaca caaatagccc
       61 gatttgatac actcaaatca ggttatttgt ggcttatatt atttacagat ttcttgtatc
      121 tgctgattcc atggcaaata agcgcgtagc gcttcaggtg tcatcgaaga cagattggga
      181 atctctgtaa aaagatgatt taaatatttg cggatgttta acccattcgc aacagctgtc
      241 tggataatac tcaatataat tccatttgat ctagcgcctt ttaagctttt agaaaatagc
      301 cagttttttc gaccgatcac taactctttg atggcacgct ctgctcggtt atttgaaagt
      361 tccaagcgtc catcaagcaa cacaatctga agtccgtctt tatgattgac tgcgtattga
      421 accgcctttc caagctgcga ttttggcaaa taagcaaaac tctcacacca atgaaaaaat
      481 tcagtcatca caggctgcaa cgtttccttc ctcgctacaa acctttcttc tgcgctaagc
      541 ttattaaagg tccgttcgat tcgaaacatc ttgtcacaga agtctaatcc tattttggca
      601 tgactttttg gggacggttg tttacccaat gcttcaaaaa actttctgcg aacatgcgcc
      661 caacaataaa cattgtttac cggcagcttt ccataggccg cgtaaccatc agtatgcaag
      721 aaaccgctaa atgattctaa aaaacttttt ggctcatcaa cggaacgcga atttgcgtgc
      781 tcgtagagca caacttgttt ttcttcgctt ttacctgaag catataacca gtaaaaggtc
      841 ttttctttat gagtattcaa tacccgatag cttgtctcat ccgcgtgtaa gacctcctga
      901 ttgcttaatt cttgatgaag gcaattgtag attggctcta aaaccatttc acttaccttg
      961 atatgccaat tagtgatttt tttacgatca agagggaaac caagtgcatg ccattctttt
     1021 tcttgtcgat atgccggcac ctttttaaca aatttttccc aaatactctg tgtcacaatt
     1081 gaagaggaac ctagactatt attgatcaac ggtttaggaa caggcgcttt tttgattttt
     1141 gtttctccta atgcttcaca ttctctgcaa tgataggcat gttggtagat gacttgtttt
     1201 tctatatggg caggaacata caatggctct tctctaagcc ttgtcgcacc gatatcatgt
     1261 aaagtatgac cacagtcttc acacaaacaa tcggcacctt ttaattcata atgaatagga
     1321 acttcaggca gaccttctaa tgaaagtgct ttgagtccct tgcgttttct gcgtttaaaa
     1381 gaagataccg taatgggttc ctggctttct tgtccagtgt gctctggttc attaaaagtt
     1441 gagtcgtcta ataaatcgat ttgcccatct acgggtggtg tttctttttt tcgtccaaac
     1501 aagcgtcgat tcatttcagc aatttgttct ctaagaagag tattttcctg ttgctggatt
     1561 tcaagcaact gtgttaattg cttgatttgt acaagaagtt cagattccat ttttgtcatt
     1621 tttacaccac cattctagcg attttcttac atctattata ccattttatg acaacgaaaa
     1681 acagacaaaa tccttgattt aacaaggttt tcgtctaata tatcgcacct tttttcgttt
     1741 cttttagcgt acatttttct tcgattgaaa ggccagtcag taagacttct tttctggaac
     1801 gcggccattg aaggtaccca ctttcaaatc ttttgtacat taatacaaat ccattttgat
     1861 cctagtaaag agccttgaag cgatctttct tcgtaccgca aaataagaaa agcgcttgat
     1921 cgtaaacatc aaggtcatat tcttctataa cgaaagctgc tagtccatca attccttttc
     1981 gaagatctgt ttttccacaa acgataaaaa tatgtttcaa tgcagttgca tcaatgagca
     2041 ttggcattca actcttttaa caaggtatac aaaagatatt tatccattcc attataaata
     2101 aatatttcct tatcttccga tttgattctg caagctagtt ttggcttggt gtcaatttgc
     2161 gatttttgag ccgcacttga aaattgaact ggtacaatat cttttttatt cattcgtttt
     2221 tctccttcta tatttgatag tttaattata gaagaaagat aatcggcgta cgagatacta
     2281 tcctatgaca cgcttacatt taatttaata ttttgcctca aattagccac actttttata
     2341 gaactaagag gaaaaatgaa gttttcttcc tattaatata gttgtttgaa atcttaaata
     2401 tagcgtttta cgatgtaatg ggaagtatgg tgaataaatc aactatgttc gtacgaagca
     2461 tttactcaac taatttaatc gaatctttca ataaacaaat caagaaatac agccaccgca
     2521 aggaacagtt ccaaaatgaa gagtcgatgg aacgtttctt agtctcgtct tttgatactt
     2581 acaaccaaaa attcctaggt cgcagtcata aaggtttcca acaggccgaa ggcgagctcg
     2641 aacaaatgct gagtcaaccg atggagaatt agaaacaatc tacagtaggg aagaaccatt
     2701 gacacaaaat tattgacgct cccaactaaa agtgtcaaaa taatgatgaa atcatttagt
     2761 tcttataaat tagaaaagag aaaataaata ttccatatct actttattgg atccgtaaca
     2821 tgactaaatt ctgaatactt tttaaagctt atcatttggt aaaactgaat ataagttaat
     2881 tatatagact gaaaattttg aattttcaga atagaataac ttaaaagtac tgacaaaatt
     2941 ttttaaaggg gcaatattaa tgttctgaga taatcaaatc ctaccgaaaa tatcaaaaat
     3001 ccaatgtgac acaatcagat tgtgtctcat tcgagggaat cacacaatcc cacgtggctc
     3061 taggcagaat tgaatttttg cttggggctt tttccttata ttgattttga atgagactta
     3121 ttatatgtta taatgcaatt aagtatcatt ggagagggcg aagaaaatga agacggtaga
     3181 acctattcga gacaaaaaaa agatcgatgc gatgaaggca atcttggctt cgggaaaata
     3241 tggccaacga aatttggttc ttttttcgat tgggatcaac accgcctatc gcatttctga
     3301 tttgcgtcaa ttgaagctgg ccgacgtgct ggaaatttca cgcggtcgcg tggtggtcaa
     3361 agatcgatta gcgatgaagg aacaaaaaac agctaaacat aattcggtct atatttccaa
     3421 caaattgcga aaaacgattt tggagtacgt tcaacaggaa tttcctgatc aactccttca
     3481 gcaagatttt gacggttatt tgttccctag tcgaaaaggg acgaatgaac cgctcaaccg
     3541 ccaaagtctg tggcgaatca ttcatgatgc tggcacggca atcggattga aagacattgg
     3601 cccacattcc atgcgaaaaa cgttcggtta ctttctctat aaacagggga ccaaaacaga
     3661 aatcattcag tccttgttga accattcttc tcagcgcgaa acattgcggt atattggtat
     3721 tacacaagaa gacaaagata cagctgtaaa aagcttagat ttataatata aaagttaagt
     3781 ttaaacatgg atagttttaa attagtagat tgagtgttgg gaggaaagaa tgagtacatt
     3841 atatattata ggaaacggtt ttgatttaag tcatggttta aaaactagat acactgattt
     3901 ttcaaaatgg gttgaaaaaa gtcatagtga aatattcgat aaaatagaca caatgataaa
     3961 tgaaaataat gaggtgagaa gtacagctga tgataagata ggttggttta attttgaaag
     4021 tagtttgcat gagttaattc aaagtccagt ctttgaagat tatgtagaag agtctaagga
     4081 ttatctcata ggttatggac atgaagattg gtcagattct gcccatcatg attttcaaaa
     4141 tatggtttat gattatgttg tttctctaga agaattacaa caacttttca aggaatggat
     4201 acgcaaagaa atacagccga caatagaaca taaaattcca aaataccaat tttcgtccga
     4261 tgtactattc tttacattca attatacaga tgttttggaa attctataca agcaaccgaa
     4321 agaaaacata tatcatattc atggcagtta tgacgatata ataatcggcc actccgagaa
     4381 ttttgacaac tttataggag at
//
LOCUS       DAFEPQ010000120         4370 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.127, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000120
VERSION     DAFEPQ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.127"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            157..1080
                     /locus_tag="LP543_002627"
     CDS             157..1080
                     /locus_tag="LP543_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBL2322749.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1086..2339
                     /locus_tag="LP543_002628"
     CDS             1086..2339
                     /locus_tag="LP543_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBL2322750.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            2473..3819
                     /gene="gor"
                     /locus_tag="LP543_002629"
     CDS             2473..3819
                     /gene="gor"
                     /locus_tag="LP543_002629"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBL2322751.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
BASE COUNT         1503 a          690 c          941 g         1236 t
ORIGIN      
        1 ttttgtcaca atctcttttt atatttggac agaatatatc gaactttatt ttgatttttt
       61 gtgaaataat tttcaaaaat ccgactttaa atagaagaag tattggaaag tttttagagc
      121 tatgctacta ttatgaagtt gtaggaggag aaaattatga tcgaactcat aaccacagta
      181 gaaactattg aacaagcaga acagttgctt gctgcaggtg cagatacact atatttcggt
      241 gaagagcaat ttggattgcg tctgcctgct tcatttacac gagaagaaca gcgacaattg
      301 gtagaattag cacacaagta tggtaaaaaa gcgaatattg ctgtaaatgg gatcattcat
      361 cctgaaaaaa tgaaaaatgt acctgaatat ttggcttttt taaaagaaat aaacgtagat
      421 gtgatcacag ttggtgatac aggtattatt tatacgatga aaaaaaatcc tgaattgcgt
      481 ataccgtata tttatgatgc tgaaacatta gtaacgagcg cacgtcaaat caatttctgg
      541 gcgaaaaaag gcgcagttgg agcagtactt gctcgtgaag ttccttttga agagatgaag
      601 gcgatggaag aaaaactcga tattcctgta gaaacccttg tgtatggtgc tacatgcatc
      661 catcaatcca aacgtccact gctacagaat tactataact atacgaaaca agacgaacaa
      721 aaagatcgtg aaagaggtct ttttatctcg gaacctaaaa aagaagagac acattattct
      781 atttatgaag atagtcatgg cacacatatt tttgccagca atgatctgaa tctctcgaat
      841 gaattgaatg aactttatca gcatcattac tgtacatgga aattggacgg attatttact
      901 ccaggcgaaa aattcgtaaa catcactcga ctattcgccg aagcaaaaaa acaaatttta
      961 gaaggtagct gggatcaaga aaaagcggaa aaaatgacta gcgaaatcag taaattgcat
     1021 cctgaaaacc gtgaattaga tacaggattc ttttatattg atccttctgc tatcaaataa
     1081 ggagtatgat gaaagaaatg acagtaaaaa gaaaattgaa acgaccagaa atcctagcgc
     1141 cagcaggaac actggaaaag ttaaaaaccg cgatccatta tggagcagat gcagtatata
     1201 ttggcgggaa tgcatatggt ttaagaagcc gtgcaggtaa ttttacttat gaagaaatgg
     1261 ctgaaggagt tgcttttgca aaagaacatg atgcgaaggt atacgttgct gctaatatgg
     1321 tcacccatga aggaaatgaa gatggagcag gggaattttt ccgcagtttg cgtgatgtcg
     1381 gtatttctgc agtgattgtt tctgatccag ctttgatcga aatttgtgcg acagaagccc
     1441 caggtctgcc gatccattta tccactcaag cttcagcaac taattttgag acattggaat
     1501 tctggaaaga agaagggttg gaacgtgtcg ttttagctcg tgaagtttca atggaggaag
     1561 tggcagagat tcgtaaaaat acggataccg aaatcgaagc atttatccat ggtgccatgt
     1621 gtatctctta ttctggtcga tgcacattgt ccaaccatat gtcgatgcgg gatgcaaacc
     1681 gtggcggatg ttctcaatct tgtcgctgga agtatggatt gttcgatatg ccttttggtc
     1741 aagaacgaaa cagtttaact gatacaggcg aagtggaaga agaattctcg atgagtgcag
     1801 tggatatgtc aatgatcgaa catattccag aattgattga aaacggcgta gacagcttca
     1861 agatcgaagg aagaatgaaa tcgatccatt atgtatctac ggtgtcaaat gtctataaag
     1921 cggctgtaga cagttatatg gaagatcctg aaaactatgt ctgcaagcaa gaatggatcg
     1981 acgagttatg gaaagtagca caacgagaat tagctacagg tttttactac aatacaccaa
     2041 ctgaaaacga acaattattc ggtgaacgtc gtaaaatccc agtatataaa tttgtcggtg
     2101 aagtattatc ttatgatgag gcaacacaga ttgctacgat acgtcagaga aacttgtttt
     2161 ctgtaggaga tgaaatcgaa ttttatggtc caggattcag tcatttcagt caaaccgttg
     2221 aagttatgca taatgaagaa ggcgaatcca ttgaccgagc accaaatcca atgatgattt
     2281 taactatgcc agtcgcacag ccggtgaaac ctggagatat gatccgaaaa cgtaaataag
     2341 gaaaaagctg atatatccct gttttaagca tcttcgcata caaaattcaa gaaaagtgtt
     2401 acacttgttt tctgtagaaa aaggatcgtt aaataaaggt tttgattgat ctcttcaata
     2461 aggaggaaca aaatgcaaat atatgattac atcgtaattg gtggcggctc tggcggtatt
     2521 gcttcagcaa acagagcagg gatgcatggc gcaaaagttt tgctgattga agcgaatgaa
     2581 ttgggcggaa catgtgtcaa cgtcggttgt gtacctaaaa aagttatgtg gcaagcaagt
     2641 acgattttgg aaacaattga acgagatgcc tcaagttacg gtatccaagc agatttaaaa
     2701 aatgttgatt tcgccggtct agtagaaaga cgagaaaaat atattgattt tctacacggt
     2761 gcttatcaaa gaggattaga tagcaatcat gtcgaggcta taaaaggtta cgctactttt
     2821 atcgatagtc aaacgatcga agtaaacggt gaaacctatc gtgctcctaa aatcttgatc
     2881 gctactggag gccgtgcaag taaaatgacc attcctggtg gagaatacgc aattgattca
     2941 aatggatttt ttgaattaac agagttacct aagagagcaa tcgttttagg tgctggatac
     3001 attgcggctg aactttcagg tgtatttcgt ggtttaggta gtgaagtcat gtgggcatat
     3061 cgaaaagaac gaccacttag aacgtttgat aaaatgcttt cggacaattt gatccaaatg
     3121 taccaagaag ctggaataaa aacttacgca catcatattg ctaaagaaat tactaaaaac
     3181 aatgacgaat atacggtgct atttgaaaat ggtgaaacct taactggtga ttgtgtactt
     3241 tttgccggtg gacgtgtacc gaataccgaa aaattaggtc tggaaaatac aaatgtggaa
     3301 ttggacaaaa aagggtttat caaagtagat aaataccaaa atacaacgga tgaacatatt
     3361 tttgctgttg gtgatgtgat cggtaaatta gatctgactc ctgttgcaat cgcagctggt
     3421 agacgtttat cagaacgttt atttaatgga aagaaagatt cttatcttga ctataaactt
     3481 gttccgacag tcgtctttac gcatccacca attgcaacaa ttggtttgac agaagaagaa
     3541 gcattggaaa aatatggcga aaatgaattg aaagtttatc gttcacgctt taccccaatg
     3601 tactttgcat taaatgatta tcgccaaaaa tgcgaaatga aattgatctg tgtcggaaaa
     3661 gaagaaagaa tcgtcggctt acatgctatt ggtgttggtg tcgatgagat gctgcaaggt
     3721 tttgccgtgg ctatcaagat gggtgcaact aaagaagact ttgacaatac agtagccatc
     3781 catccaacag gagcggaaga attcgtaacg atgcgataat ttaatattaa cagttccaac
     3841 agctttttat tgaacaaacc atgtaaaacc agaaaatcag tatactgatt ttctggtttt
     3901 ttgtttttta gttatttatg taagattgct tcgataattc tcaaaacgaa ataagtaggg
     3961 aagttggaga ttttgattat cttcgtaact gacgaagagt aaatgaatcc atttggtatg
     4021 cactcttaag ttcaaaatat tggtatcgat gtttaaaaga aatagaaatg tatcgaagaa
     4081 cagatgttta aaaaagtgtg actttgtata aataaattag aagaagcaaa cgaggatgtt
     4141 aaaagaatac catatagtta ccaaaccaag aaagttctcg tcaagttcgc tggtacgaat
     4201 caaatcaagt gaaaaaaggg actgtgaaaa aaattgtgtc atagttagtc actttttccg
     4261 aacaaatagt gttcaatcag ctgtccttcg gtattagctc aaaaaacggc aaaatatgag
     4321 gaactatttt tccatttgtt tctctaatac tcaggacaaa acactgattt
//
LOCUS       DAFEPQ010000121         4345 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.128, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000121
VERSION     DAFEPQ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.128"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            178..774
                     /locus_tag="LP543_002630"
     CDS             178..774
                     /locus_tag="LP543_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL2322752.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            898..2697
                     /locus_tag="LP543_002631"
     CDS             898..2697
                     /locus_tag="LP543_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBL2322753.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2975..3187)
                     /locus_tag="LP543_002632"
     CDS             complement(2975..3187)
                     /locus_tag="LP543_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322754.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3649..3798
                     /locus_tag="LP543_002633"
     CDS             3649..3798
                     /locus_tag="LP543_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322755.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            complement(4051..>4345)
                     /locus_tag="LP543_002634"
     CDS             complement(4051..>4345)
                     /locus_tag="LP543_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL2322756.1"
                     /translation="DCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRD
                     GLLKSMDFNSVDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1292 a          745 c          755 g         1553 t
ORIGIN      
        1 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
       61 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
      121 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
      181 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
      241 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
      301 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
      361 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
      421 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
      481 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
      541 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
      601 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
      661 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
      721 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
      781 ttttgtaaaa ttagtcattg attgacaatg ttaaatctgg gaaagggtct gcaaaacaca
      841 ctgcaccctt tcctttttca ttagatttgc gctacaatgt gaagagcagg tgataggatg
      901 aattatttga aacgtagctt aggtaatgct tgggactttt taaaaggaac acaagcttat
      961 ttccgtgatg tcttgctgat gcatggcttt attttattta tttgtttacc cttattgagc
     1021 agtacgaccc gttttatttt acaccgaggg gcaattgact atttgtctta tgacaatatt
     1081 ccttcaattt tttctcaaca tcctggggtt ctactctcgt taatcgtagt tttgatgacg
     1141 attttactgt tagtttattt cgaatttact tttcttttga tgagtgtttt ttttattaaa
     1201 aagaaagaac caatctcttt gaagcaactc ttacacttga cgatacttca gctgaagaaa
     1261 gtgagaccca ttactttttt gtttttcctt gcttattttt tattgattct accgatcagc
     1321 ggattaagtt ttaattctga tctactgtca aaaataaaaa tccccgcttt cattatggat
     1381 tttatttttg ctaatcgttg gatcatcgtc agttcctttt tactcgttta tattttcctt
     1441 ggctatattg ggattcgact gatttttgct ttaccggaaa tgattctacg ggatcgtcct
     1501 tttcgagcag ccattcggga aagctggtca ttgaccaaat cgcgtctttt ggccatcacg
     1561 ggccaattta ttgtgattgg cggtacgatt ttactccttt cttctttagg atatatcgtt
     1621 gttattttgg cacaaagtat ggttgaacaa tttttcccag actatgcatt gatcagcgct
     1681 gtgtttgcta tgacgctttt acaagggatt ttattattca atatcgtgat gtctaccgta
     1741 gggatttttt atatcattgt tgatttcatg gatgacgaag gcttcttgcc tgagatccct
     1801 aagtggttta tcccacaagc ccccaacttg cgtttctctg cgctgaaaaa cactggtttg
     1861 actctttttg cagttttttt cggtattggc gtctgtcttt acaatatgga ttatctgact
     1921 tcagctgttc agacaaaacc agtgactgtc tctcacagag gtgtgagtac ccaaaatggg
     1981 gcgcaaaata ctttagaagc acttgaaaaa acaagtagag actatcatcc tgattatgta
     2041 gagatggatg ttcaggaaac aaaagatggt catttcgtag tgatgcatga tgcaaatctt
     2101 cgtcacttaa caggggtaaa tggaacacca caggatctta cattgaaaga attgacaaat
     2161 cttacagtta ctgaaaacgg agtgcaagcc cctgtttgtt cttttgattc ttatttggaa
     2221 aaagcacata agctaaacca aaaattactt attgagatca agacctctaa aaaagattcg
     2281 gatgaccttg tcgaacgatt tgtccataaa taccgcaaag ttatcttaga gaaccatcat
     2341 attttgcaaa gtttgacgta ccaaaccgtt tcagatttaa aagaagcaga accaaacttt
     2401 tatgtgggct acatcttacc tttcaatatt gttgggcccc ctgtgacgcc tgctgatttt
     2461 ctgacaatgg aatatagtac gatcaaccgt aacttcatta attctgccca ccaagacgga
     2521 aaagaagtat atgtatggac aatcaacgat tcagatgcta tgagtcgcat gatgttttat
     2581 ggtgtggatg gtatcataac cgatgagatg ggactactaa atgaaagtgt gaaacaaaca
     2641 gatgaagaaa tcacttattc agataaactg ctaaattttg ttttgggagt cggctaatca
     2701 aaaatcgtta atgaaaaaag tctttataca acaaaaaacg cataacctgc caccgcaggt
     2761 atatgcgttt tttgtttgac gctcataatg tgtgcttaac tttctcttga ttccttcatc
     2821 gcacgaacaa tagctaaaat agcaggaatt gcagagatca ctgcaagaat aattcccatt
     2881 ttccctgaac tttttctttt tggcatatct tctcgctccc ttctgtcttt atttatatta
     2941 taagggagtt cttgcgaatt ctcaaaaata ctgtttaaga aaccaattca gcaggtgcgc
     3001 taatatcaca gaattttacg agcatgcgat acttgctttc attgataagt tgatagtctc
     3061 gttggtcata actttcaaca tacacgacag ccgtgttgat atatgtacgt tcaatacagc
     3121 cgattagttc atgagatact ccgatcggtt tgcatatata agatcttcct acttctgctt
     3181 ttaccatttc attccctctt ttctttattt ttattttgtt ttttctttat tttataataa
     3241 caaattacct tgtatttgaa aacgttttta tccatttttt tattttttat tttgagtatc
     3301 tttctgcttt ttagataact attttttaac acgatcttag acgttgtgac aaaaacgttt
     3361 gtttatattc ctatgttgtt cgtgcgtttt tttctttata ttgctaagtt atttataaag
     3421 aggtgagcat atgatttata ttcaagcaat agtatttgta accttgatga ttgggctact
     3481 tatcggatta aaagaaaata gctaaaccat ttaagaaaaa ataaataagg aaaatgataa
     3541 atgcttttta attgcatcaa aaaacgtacg atgctaaact tggtataagt tcgaaagttt
     3601 aaaggaaact ttcaagaact aaattacatt acaaaggaga tgaattttat gacgtttaca
     3661 aaaacaaata tgcaataccg cgtagctttt gatacaactt ctaaccgttt tatggcgatt
     3721 gatgcacaaa acgaaaataa cgttgcgttt ggtattacaa tcgaacaagc agtccgagca
     3781 ttaaacgaat ctagataagg agtgaaccat tccaaatggc gactgatgag atctcggaaa
     3841 atcaataaaa gacgtttgta acggtaatcc gcgacaaacg tctttttgta ttcattacat
     3901 atatttggaa attgtctctc tttatctcta gacctaaaca aaaacacaag ttctaatatc
     3961 aaccatctcg ttttttatga caattttgtt agctgtttcg atgataatat tccgttatta
     4021 tctgtataat atttaatata atcttaattg tcaaattaag ttagacagat catcaatact
     4081 tacgtaactc aaaaatactt ccaaaggtgt tcgataatcc agtgattttc taggaatatt
     4141 atttcgttta gatgcgacag attgaataaa aaattcatct actgaattga aatccataga
     4201 tttcaataaa ccatctctac gtaacaatcc gttagaattc tcgtttaggc ctctttgaga
     4261 cggcgttcct ggatcagcga aataaatggc aatatcattg acattactga tctgtttcca
     4321 atttgaaaat tcctttccac aatca
//
LOCUS       DAFEPQ010000122         4324 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.129, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000122
VERSION     DAFEPQ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.129"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(312..842)
                     /locus_tag="LP543_002635"
     CDS             complement(312..842)
                     /locus_tag="LP543_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL2322757.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(1024..1680)
                     /locus_tag="LP543_002636"
     CDS             complement(1024..1680)
                     /locus_tag="LP543_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBL2322758.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(1807..2280)
                     /gene="dfrF"
                     /locus_tag="LP543_002637"
     CDS             complement(1807..2280)
                     /gene="dfrF"
                     /locus_tag="LP543_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBL2322759.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(2699..2917)
                     /locus_tag="LP543_002638"
                     /pseudo
     CDS             complement(2699..2917)
                     /locus_tag="LP543_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2948..4162)
                     /locus_tag="LP543_002639"
     CDS             complement(2948..4162)
                     /locus_tag="LP543_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBL2322760.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFRLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1324 a          924 c          639 g         1437 t
ORIGIN      
        1 ttttttgaaa tcaactgtct ttctcatatt tactattcaa ttttcatagt gatataggta
       61 caatttatat cactactgta aaaatggggt tttgccttat aaatacagcg ttttccgctt
      121 gttagtgtat agtgtttctt gttttttgtg ccccccttgt tagataacgg gggctttgaa
      181 cggctcgcaa gctcgccgaa ccgccgaagc gaaccgcccc cttgcaagca tggcggaaaa
      241 atcttaagga ttttcccgcc aacgtggtcg gatcgctccg tcggtttaac agcttttctg
      301 tttctctcta gttatctacg catataaatc aaatcggtta ttccgttata agtctgtgtt
      361 ttacaaagcc ttaactttat ttccctgtct atgttgccca aaagacgaac cccatttcct
      421 aacagagtag gaataacaga aatgtaatat ttatcaatca tatttttgct taccaactgc
      481 tgaacaagat ttgctccgcc acaaatccaa atatcctttc ctgcttcttg ttttagccgt
      541 tccaataata aaacaggatt ttcacttgta aaatgaattt gttgggaaga attgcactct
      601 ttatgtgtta atacataagt tgtaaattta ttatataccc attcttgcgg agaaagttca
      661 gtaacaacct gatgagaagt attccacccc attaagacag tatctatatc tttggtaaat
      721 tctgtataag tgtcgatgtt ttcattgtca tttccatgac catttagcca agctactcca
      781 ccattaccgt ccgcaatata tccgtcaagg ctcatggcaa taaataaaac tacttttctc
      841 attttttctc ttttataaat acaaacagga atttatttct cttttttact cataagggaa
      901 caactgattg ctacaaataa acccattaaa atccatggaa atgctgctct tataaaatca
      961 ctaaccatac tttattcctc cttaaattcc aatttcctta gttctacaaa ctgaaatttt
     1021 tgcctatgaa tgtatcttgt tctttatacg caaacaggct tctgtgtgca taatccaatg
     1081 tcttgaaaac ggcatttgaa ttagtccaca aatatcttta ttacaccaat aatcaatcag
     1141 ccaaattgct ttttcattgt cgtttactac attcaacgat tctaagtatc cttttctttc
     1201 tttcggtatt tttcttttca attcatcata agataaccgt tcaagcattt tctcagtgct
     1261 ttcccatact tcgaaaatat ataaatataa ttcttccaga ttaagctgtt ttgaaaagtc
     1321 tgcaatctgc tgtttcatga gttcatttcc tgttgtaatg ataggtgaat tgatacgctc
     1381 ttgataattg cctgcaaaaa atacttgttc atcttcattt atcactgtat gcacaactat
     1441 atcttcaatg cggaaaatat gccaaataga ataagcaatc gtcttactat gataaccatc
     1501 agcgtttatg aaggggattg agttaaaatc ttctctgcat aattcttctt tgaaagatac
     1561 taaggtttgt attaactgac ttcgtaaagt aagtaaagta tcaatacccc tcttataagt
     1621 atctttcttt tttatttgtg cctgcattgt tttattaagt tcagaccatt ccttattcat
     1681 attcaatcct ctctcaaatt ctgattttat cgccccatac aaactggaat ttgtcttaca
     1741 acaatttata tatgcataaa aagcaatata tttatgaaaa caccctaaat ccaaaattta
     1801 ctcaattcat tttttcctta cataaaatgt tctcgtatat ttcacttcct caccaagtgt
     1861 ttcccctatc aaaacttcaa aatcattgat atcaaattct ggaaagaatg tatctccatc
     1921 ttcaatattt aaatctactt ctgtgatata cattttatct actatttgca aagcttcctt
     1981 aaatagtcca tatccaccag atatgtatac atctcgtcct ttagccaata ataatgcatc
     2041 ttctaatgat ttaactgaaa ctaaattatc tccttgatac tctgttgtgg tggaaacaac
     2101 aatattcatt ctattaggca acggatgacc gatttcttca taagactttc gccccataat
     2161 aaccacatta cccgttgtta actctctaaa ttgcttttgt tctcccttta ttttccatgg
     2221 tatattacca ttcttgccta taacattatt ctttgacctc gcaacaatca aacctatcat
     2281 ctatttcatc ctccataata tgatgtcgta cctgtgattc catcaagtgc accacatctt
     2341 aacaaactca aaattatctc gaactaacca gctcgacaaa ttcctttttt gtcttcatct
     2401 gcattatatc atattgttat aagcattgcc atttcttttc tgcttttgaa aagttactta
     2461 tcagcctatg ttttgtaaag ggtgatattc agcatttctc acgcaacaag cccttgacaa
     2521 accatagact aataagtttt aaggtaatca agcagaaatg aaatagtctg ctgtttcacg
     2581 gcaacaaaaa agagatacca acacacttat gtagtatctc ttagtctagt agcaatattc
     2641 ttttgtttgc aagcaggtaa acatgggctg tttcttaata tctttttgtt tgggaaacac
     2701 ctttttccca tcctttcacc gttgccatct ctgctgcgca caatgccaca gggataaacc
     2761 atagcaaacc gcctaataac aagaaaaata ctaagctgaa gcctgaggtc gcgaatgtgg
     2821 gatattcata taatgaaacg accattgaga tggtcatagc caaaaagcca aagaacgtta
     2881 atttttttgc aggaattttc gttttgcttt gttgcatgaa tcttcctctt ttctatttga
     2941 atttgtttca attgagtttg ttgccaaaga agaaaaagtc caatccttct tcttgttctt
     3001 cttttgtagt agtgatcgtt gtaaagtgca tcgccgattt taaaatcact gttgtcagca
     3061 cataggcata cacggcagta atgatgacac caatcacttg aatccctaag aagtaccagc
     3121 tgcttggatt cgttagtaaa tggctgttag atgcaaagat ccctatcaga atcgtacctg
     3181 tgaatccgcc catgccatga acaccccaca catcaagtgc atcatcccat tgtttctttt
     3241 tacgaaaatc aacagcagca tgacagacga tacccgcaat gatcccaatc agcatggcac
     3301 tttttgcttc tacataaccg gcacaaggcg taatggctgc caatccagca acggatcctg
     3361 ttagtacgtc gacaaaatca aagccatttc cttttacttt tgcaatgatc agccaagtaa
     3421 tcatcgcaaa tgcaagagcg atgaatgttg aaccaaatgc attgactgct tgttcatctg
     3481 ctcgaagtgc accaccggaa ttaaaaccga accagccaaa ccaaagcagt cctgtaccga
     3541 tagcagctgc cataagactg ctatgctgac ttctttctcc taaaggaatc tttctttgac
     3601 caagtgtgag gatacatgct aaagagccga atccagctgt cgtgtggatg actgctccac
     3661 cagcgaaatc tctaaatccc atctgatcca aaaatccgcc accccatatc cagtggcaaa
     3721 cagggaaata aatcagcaga ttccaaaaaa taacaagtat aatatagcct tttaaattca
     3781 atctccctgc aaaagctcct gtcatcaaag gaacggtaat cacgataaac atcagttggt
     3841 aaagaaagaa cattaaaaat ggaatcgttg caccatgaaa catgttgggg aagcttgtaa
     3901 catgtctcat caaaaagaaa tctgccgggt ttccaataac accgccgata tcccgtccaa
     3961 aggccaatct gaatccacca aatacccaca taatcgttgt tatcccaacg gagagaaaag
     4021 attgagacat gatcgttagt atattattct tccgtgctaa tccgccgtaa aaaaaagcta
     4081 gaccaggagt catcaggcaa accattgctg tacataaaat catgaatgct gtatctcctg
     4141 tattgatagg taatgtattc atttttttcg cctcctttct acgattacga gtataaaaga
     4201 aggtgtagtt gtttttttct tctgtttttt tgaattaata cttgcattta taaaagacag
     4261 acaattaata aaagcttgat gtgttgtgct ctttttcgtt tttcacacaa ataaagacaa
     4321 aaaa
//
LOCUS       DAFEPQ010000123         4269 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.130, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000123
VERSION     DAFEPQ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.130"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(178..420)
                     /locus_tag="LP543_002640"
     CDS             complement(178..420)
                     /locus_tag="LP543_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL2322761.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(452..1009)
                     /locus_tag="LP543_002641"
     CDS             complement(452..1009)
                     /locus_tag="LP543_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL2322762.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(1022..1210)
                     /locus_tag="LP543_002642"
     CDS             complement(1022..1210)
                     /locus_tag="LP543_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBL2322763.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(1223..1789)
                     /gene="amaP"
                     /locus_tag="LP543_002643"
     CDS             complement(1223..1789)
                     /gene="amaP"
                     /locus_tag="LP543_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBL2322764.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            2206..2379
                     /locus_tag="LP543_002644"
     CDS             2206..2379
                     /locus_tag="LP543_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322765.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            3019..3492
                     /locus_tag="LP543_002645"
     CDS             3019..3492
                     /locus_tag="LP543_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBL2322766.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            3501..3728
                     /locus_tag="LP543_002646"
     CDS             3501..3728
                     /locus_tag="LP543_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2322767.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         1292 a          762 c          638 g         1577 t
ORIGIN      
        1 aaacggtctt ctttttatag aaaaccgttt attttcgtct ttttttattt ttaaagatat
       61 tttttcataa ggaataaaga atacttcata taaaatatat aaaaaagaac cgcagcgagt
      121 gtcgctacgg ttctcaaatt tgttcttcat ttcaacataa gtttttcttc aatttgctta
      181 taatttacgt gcaaagaatg aaacgactaa tactaaaatg attgcaccga tcaatgatgg
      241 aatgatagcc attcctgcaa ttgttggtcc ccaagttcct agtaaagctt gtccaattgc
      301 tgaacctact aaaccagcaa tgatgtttgc gatgatcccc attgatgatc ctttattcgt
      361 gattgctcct gcaattgctc caatgatacc gccgacgatt aatgcccaaa taaatcccat
      421 tttaaattcc tcctaactga cttcttactt cttattgtac acgtggttcg ttattttctt
      481 tgactttttg tgcacctttg cctacagcag attttgcttt atctgtttgg cgtgaagcaa
      541 attgtcctgt ggattttgct gcttctgtta ctttgtcttg tactgtttct tgatcttttt
      601 ggtattcttc ttgtgttttg atgtctgcaa cattcacatt cacttcgata acatctaaat
      661 gtgtcatatt gtttacttca gtactgatca attctttgat ttggttgtaa atatcttcga
      721 tgtctttacc gtattctacg atcacatcta gatccacagc tacttgtttc ttacctactt
      781 ctgtattgat acctgctgtt acgttatctg tgttgactaa tttttcagcc atatttgaga
      841 aaaatccgcc atctaatgtc aatagaccat ctacatcttt caatgcataa ccgatgattt
      901 tttggatgac tttgtcttca taagtcaatt caccattcat catgcggttt tctccttttt
      961 catttgccaa aactgatcca tttgttgtat tcatgttttt gttttccatc tttgtttcct
     1021 cctatgattt aaaaaattga tcaactattc ctgactgttt caaataaaaa ccgattcctg
     1081 cgccaattgc tgtcatgatt atgatcaaca atgtcttgaa aaatccaatc gttaaccata
     1141 aaacagctag cagtagtcca agacccccaa agataatagg gagcttgtat gctgaaaata
     1201 gttctttcat tatttggccc tcctattcca ctcgagagtg cttgtagtct cttgtatctt
     1261 cttgttcttc ataaccgctg tatgtgactg ccactttgac cgtttctttt gttccgagta
     1321 ctttagttac ttcttcttcg atatcttgca tcaatacacc tgttttccct actaatgaag
     1381 aagttcgttt caagtctccg ttgacatgga tatggattcg atttttcgtt gaacgtatcc
     1441 gtactttagg tgaatgaatg aactcttctt cgtgtaaatg ggaacgtacc aacccttcga
     1501 tcgctttttt gtcgattgtt agtttgccat cttcccgttt cataacgaaa gttcctcttg
     1561 actttggata gaaaagaacg actagcaaga tgataatagc aagaacggca aatactacag
     1621 ctgcccagaa aatatattgt tgcatgtagt aattcgttaa agtaaagaag cgaaatcgtt
     1681 ccaattgttc aggcataata tacggcgcat tgatcaacgc aatcgctaga aatactgaaa
     1741 gtagcagtaa actcatgata acaccgatta tttttaaccc cttattcatt ttccacctcc
     1801 tataaactta tttttaccct gtaaataact ctaccgacaa tcacagtata gatagcgaac
     1861 agttagcata gcatcagtaa ataaagcgct accaaacatt gctggatcat aacggcgttt
     1921 tattgccagt ctgatactga aataacaatt gcttacttat tctctattta cttttgattg
     1981 ctggctatac ttaaagtata ttattcaaga ttaaaaattg caaagattat gctcagttat
     2041 tattacaaga ttctatttat ttattaaatt ttttcggaga attaaaaaat acaataaata
     2101 actttagtta ctttaatgcg ttcttacttt atttttatat tatcattttt cttattcact
     2161 tctttggttt atactattct tataaataaa aacgaggtat gtcctatgat gaaaagattg
     2221 tactattcat tgatcataac gattggctat ttgattgtat caaatctagg aaatatggta
     2281 tttggtatca gtaaagaatt cagttggact actacattat gggaatctct attctttttc
     2341 atcttcgtat ttttgttaca aaattatcga aaaaaataaa catgtgccat ctatctgttg
     2401 gcacatgttt taggatttat ggtacttttt attcggcaat ccattttatt tttctacttc
     2461 ttcttaatac ctctaacact tttgatttat aaactaatcc aatgcatcta cttgcgctta
     2521 ttaagctatt tcagctagat agtaccaccc catgcgtcaa tgcttgattt tccttttgta
     2581 taacattgta taagcaataa aggacaaata agtggtgatt acaccaaacc attgaataag
     2641 aaaaattctg atacgtaaat aaacatgaac ttgaaaaaat taatgaatcg gttagctact
     2701 acgtagttgt cttttttctc atacagttct gttaacttac acttctcttc gctagataat
     2761 aaaaaatcct ccaactgtat tgtccaatat aatcccaggc atctaaacta gcattataga
     2821 agagacacac atagaatcta aaataactgg ttttaaatgt ttcttacaat caaaaaaagc
     2881 tgttaaatgc actccacacc ttcatactct atcatttttt ataaacgata tatttttatt
     2941 gtttttcgat aaaaaatagt tgttttacaa taattattct gctatatttt agctatcaaa
     3001 gataaaagag ggataaaaat gaaatttaaa acattgctat taaaatttgt cgttttaggt
     3061 ataagtgccg tatttatttt gttcggcgta cttttttata ctcagatcgc tacaagcgaa
     3121 aaaggattca attttgattg gcagaccaca ttatttgttt ctgtcattta ctttgccctt
     3181 ctcctaggga tcgttattgc ttatttctta ttgaaactat tgaaaaaaat cgaagataac
     3241 caagcttttt ccactcaaag cttaaacata ctaaaaaaaa tcgaacgaac cattttgttt
     3301 attggtttcg ccttttttgg cttacttcct aaatttatga aagtagcgga tttagacgat
     3361 gctccaggtt tgatgttggt aggaatcgct gtcgtattca ttccttttgc agtatacaca
     3421 ttaacaagcg tgctggaaaa actgcttgaa catgcgattt tacttaagac agatcatgat
     3481 ttgacagtgt gaggtgcgac atggcaatta tcgttaattt agatgtagta ttagctcaaa
     3541 aaaaaatgag tgtgactgaa ttgagtgaga aagtgggcat tacgatggca aacatctcta
     3601 ttctaaaaaa tggaaaagca aaagccatcc gtttttccac tctcgaaaaa atctgccaag
     3661 ttttggagtg tcaaccaggt gatttgttga cctataaaaa tgactcgaat actataccag
     3721 aagaataaaa taaagtagaa caaacagtaa ataaaaaata atctgaatga tagatgtgtg
     3781 agacttttag aagttattta cgtctattat tcagattttt tatttcgatt gaataccgtt
     3841 ttatctataa ataagatatg tactgactca tacaaagggg gccttttctt ctccatgtac
     3901 tatcactttt cctgatacaa ctggtttcct gttacgttgt agttggatct gcttttagta
     3961 taatacttta ttcattccac cagactgata gcagtttgag cagtctaaat aatcatgtag
     4021 tattacaggc cattctgttt cataccttga agtgcttaaa acttcagcta atggcccatt
     4081 gtgttaactc ttactccatt cttttttagt aagctcccat atatactgta aagccttcta
     4141 tatattatgt catgttgctt tttaacattt actatgtagt tttgataaaa tgatttgaat
     4201 aacagtgatg gacaattctt ttttattttt aacattattt tgataataat gacagactgt
     4261 agacaaaag
//
LOCUS       DAFEPQ010000124         4243 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.131, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000124
VERSION     DAFEPQ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.131"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(76..1884)
                     /gene="pepF"
                     /locus_tag="LP543_002647"
     CDS             complement(76..1884)
                     /gene="pepF"
                     /locus_tag="LP543_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBL2322768.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2060..3058)
                     /locus_tag="LP543_002648"
     CDS             complement(2060..3058)
                     /locus_tag="LP543_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBL2322769.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3284..3937)
                     /locus_tag="LP543_002649"
     CDS             complement(3284..3937)
                     /locus_tag="LP543_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBL2322770.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         1244 a          896 c          658 g         1445 t
ORIGIN      
        1 aatacgtcga ctcccttaca agagagccga cgtatttttt tcgtttctat tcgagaaaat
       61 caaaaataga aagccttata aacgatcgat caattcttct aattcattta gacgttgttc
      121 aaacatcgac attgcatctt caatatatgc agcctgtgtc atatcaactc ctgctttttt
      181 cattacttcc acaggataat cgctgttacc tgcttttagg taagccaagt agttctctag
      241 tgcctctggt tcctggttta gtattttctt cgccagtgct gaagcagcag aaaagccagt
      301 cgagtattgg aaaacatagt aattataata aaaatgcggt atccgtgacc attcgaattt
      361 aatttctggg tcttcttcga ctgctggacc ataatatttt gcattcaatt taccataact
      421 atcacttagg tattcactag tcaatggcac acctttttca tcttccgtat gcatgaaatg
      481 ctcgaattca gcgaattgtg tctggcggaa aactgttcct ttaaacccat ccaagtaatg
      541 gttgagtaca taagcccgta cacgagggtc tttttctgtt tccaataaat actccgttag
      601 gatattttca ttcgttgtcg aagcaatttc tgctaaaaag atggagtagt cgccgtacac
      661 ataaggctgg ttcgaacgag tgaaataact atgaacacta tgtcccattt cgtggacaag
      721 cgtaaatagc tgatccagcg tatcatgcca attcaataaa atatatggat ttgtgtcata
      781 gcttcccgaa gaataagcac cgcttcgttt ccctttattt tcgacaacat cgatccaacg
      841 ttcagagaat gctttttcta cgatggccat gtattcttca cccattggtt tcagtgcttc
      901 taaagctttt tcttttgctt cttcgtacgt aaaggtaatt ggagcttccc ctaagaccgg
      961 tgtataaaga tcatacatgt gcagtttttc cacttctaat aaacgtttcc gtaattccat
     1021 gtatcgatgt aacaaaggca aatgcttgtt taccacgtct accaaagtat cgtatacact
     1081 ttcaggaata tgattattgc tcaaagacgc ttctctggca gagctgtaat tacggacttt
     1141 cgctttaaaa ttatgtcctt ttatatgtgt gcctaaagta gaagcaaatg tatttctaaa
     1201 ttgttcgtaa acactgtaca atcccttaaa tgctgcttcg cgcacccttc gatccgtgct
     1261 ttctagcaac tgaccataca cgccatgaga taattggact atttcaccat tttctccttc
     1321 aatcgttgga aaaactagat ctgcattatt caaaacagca aatgtatcac ttgaagcatc
     1381 aaagatttca cctgctccag caagaagaga ttcctgctca gcagataaga catgggctcg
     1441 attatctact atttgctgga tataatggcg atagacttcc aattttggtt cttctttgaa
     1501 atattgccaa atctggtcat ctgacaattg caatatttct ggttcaaacc aggaaacggc
     1561 ttcactaact ttcgaaaaca gactgcttgc tcttgcataa agcgcttggt aatctgtatt
     1621 tccagtgtct tgatcgtttt taaggtgcgc atatacataa atgacttcag tttggcgata
     1681 aaccctcaat acgaattcaa tcgcatttaa aaattgagaa gcgccttgat caagtgttcc
     1741 tttgtgtttt tcagattgtt ttaactcttc tgataattcc aaatatttct catcaaactc
     1801 ttggtcgctt gaaaagatct tggtcaagtc ccaagttaaa ttttcaggca attcttctcg
     1861 ttttggcaac tgctttactt ccatgaaatc cactcctttt cttcccttag ctctatctat
     1921 caaaatagat catagtctaa catccacatg tcacagcatc taacttttct actcattcat
     1981 gtgtctgctt ccatcaattt tagcataact aaatcttgtt ttacatagaa aaccgattcc
     2041 tagcctattt ttatacttac tattttttca aagaagacaa gtgtatgcat tcgttaaaaa
     2101 actgctgata gaccatctct tcatcaatca taggaaaaag ccattccctt ttatgttctt
     2161 ttatttggca aagccatgag ttgaaatcag gaactttctg gttttccaag gcttcttcgt
     2221 acaacattcg ataaagaaaa acctgttctt gtaaataaaa gaaaaaccga cttttttgat
     2281 agatccacga tgatagatgc aaaagatgtt tatgacgcaa gtagcattgt tcttgtatgg
     2341 atactgtatt cttttgcttg tgaataagtt ggcgtgtcag ccatttcttc ccatcttcac
     2401 tcactttttc tatttttttc atttggttca ttttaatttc gttactagtg aatatttctt
     2461 taaggtccct actagaaaaa gtccagcagc tactgcaaaa ggaaatatgc cctgatacag
     2521 tctctgtaat atgatgatac aaataaagct tttgtttctt gagatcagct ttccagcaat
     2581 gcactcctct ttttctatta tagtagcagc aacttttttc tatcaatgag aattgcgatt
     2641 gtccaagaaa agaattacca agtatccacc atacttggta actgttattc aaataatttt
     2701 gggttctttc aataaatctc tggtgagaca gtctagaaca ttggatttct attgctagat
     2761 tcgccaacaa aatatccggc ctttgccgca acctcggcaa ataggcttcc aaaaatactg
     2821 gttctactga ctgtcccagc caatccatga agcattcctt taactgtaag tgctcttttg
     2881 attctctttc actaaagacc gaacagttat ctgatttttg atgggcaaaa tgagcacatt
     2941 ttttctttcc ctttttcaaa atcactcttt ttcgacagcc acgacaataa tatttctttc
     3001 ctttttctgc atgtttagca aaacagcact tattatattc attcattgca agcagcattt
     3061 ttctccctct gaacttattt acgtctaaga atttatttac acgaaaaaag agggcgtgaa
     3121 aaagctattc tgcatcaagt aataagaaca gctaaacaaa aatagttccc aatatttttc
     3181 gtttggttgg gcttgttacc gcagatgcaa gcttttgaac acatttatac gaaaaaaaga
     3241 ctgcgacaca atttttgtca cagtctctta ctataatgct gatttataca aaataaaatt
     3301 tcgttcgctc gatagcatta cgcgccatca gacattgtcc atattcattc aatgtttctg
     3361 gtgtacgatc gctcttttca gcaaactcca aaattcgagc aatctgattc tctacatccg
     3421 cttggttgag attttccatc cagaagtgaa cgactaggta atagcgctct tcgaataaat
     3481 acaaatcagt ccaagcagac tctagttcga catctgttgc aagttggatc acttgttcaa
     3541 aatcttcaaa agcaaaagtt gcttgtcttt cctgttcttc tagggcgctt ccagataatt
     3601 cttcctccgg atcgctgcca ataatctgct gacgaagaaa gtcactcaca tcttctgaat
     3661 caacagtaga atcttctgat tgagaaattt cttcatccgg aagattttta ctgataaaca
     3721 gttctaatcc gtctcctttt ggcaaaactt ggaaagtgac agcttcgctt cctcggaact
     3781 cctcttcgat atcaacttct tccaaaatac tataaaagaa attttcgatt tctttgtgat
     3841 tgccaagtaa atccaaaaat gaaacgcccc gttcctctaa atcttcatga ccaattagta
     3901 cacggatcgt attttcatta atatgttcca tttccatttt gctacacctc actttgccac
     3961 aatgtttaat atagattcat tgtagcgaaa tcagcttaaa tgtaaagaaa aaaaatctcg
     4021 ctgttaagcg aatttaataa aaccagcgga tttttcaacc tttcaagcta tcttatttaa
     4081 ctataacatt tttggcagac gaatactata tagccgactt ttcagctaat tttccctctc
     4141 tatttttcgt gcaatcatca tcttctacta cctttttata catctaatca agaatacttt
     4201 ttgtaaaaac aaaaaaactt ggaaaaacta tctttgtaat agt
//
LOCUS       DAFEPQ010000125         4222 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.132, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000125
VERSION     DAFEPQ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.132"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..343
                     /locus_tag="LP543_002650"
     CDS             <1..343
                     /locus_tag="LP543_002650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322771.1"
                     /translation="NIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQDK
                     KNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQKK
                     RDNNDQYLPKK"
     gene            424..1044
                     /locus_tag="LP543_002651"
     CDS             424..1044
                     /locus_tag="LP543_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322772.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     EIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQIS"
     gene            complement(1374..2600)
                     /locus_tag="LP543_002652"
     CDS             complement(1374..2600)
                     /locus_tag="LP543_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL2322773.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(2672..2830)
                     /locus_tag="LP543_002653"
     CDS             complement(2672..2830)
                     /locus_tag="LP543_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2322774.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            3518..>4222
                     /locus_tag="LP543_002654"
     CDS             3518..>4222
                     /locus_tag="LP543_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL2322775.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFI"
BASE COUNT         1449 a          590 c          689 g         1494 t
ORIGIN      
        1 aaatataaaa actagtacga aattaatttg taaattattt aagcttgata aaataagcaa
       61 tatcgatgat gtaaagtcca agatattaac aagaaaagta agcgaaccag taaagctaac
      121 ggcacaagat aaaaaaaatg gaattgttct ttatggtgat gatgcaaagt ataactataa
      181 ttgggatacc tcctcagaag ctgaggtatt aagtattcct actaccgata cttcagggga
      241 aaaagtcacc tttaaaataa attttataac ggcaatttct aagctggagt atgggaagca
      301 aaaaaagaga gataacaatg atcaatatct acctaaaaaa taacgaatta atgatgcata
      361 tgttaaagtt gtttttttga attaaacaac tcaatttatt cagttatatg ttcatcgcag
      421 caagtgaaca ttaatcgagt tataaaatta ataggagcta aaaatataaa taaagttcga
      481 tcagaattta tttttccaga agattttttt aactggtttt tttatcaatt cagtgaatgc
      541 aagcgactct taggtgagaa ttattactta aaaggaatcg gcggatttat ggggaatgtg
      601 gctgatgaac aaaatattat aaaaggaata tctgaacaaa catcagagct tacagtaact
      661 aaagcattta taagtaatgg tgaaagtttg aaaaatgtaa ctgcgcgaat tagaaaagaa
      721 aatgaagtag aaattgtttt tggaattgac catgaatgta attcagtgat atatgtaaca
      781 caatcagcaa atttaattct ttttgagaat ggttgcaagg acgattttat ggtggtctat
      841 ttgtacgata tactaattcc aaaacttcgt tccttatata tgtcttctga aagtattttt
      901 gtgagtgaac aaaagggatc tttctcgaaa aaaataggta ttgaagtaat tgaatcgatt
      961 atgtgtcata atcatattac gctagaagaa ttagaaattt tgatagggca aaaaaataat
     1021 aacacgaaaa tacaaatttc ttagaaatca ttttttattg agatatctag ataaatgatt
     1081 tattcttcaa tcaaccaact cgccaagctg aggtaatgtt ttgaggtaat gataacatac
     1141 aactttttat accctagtaa aactctataa acaaagactt attaatttcg catgaaatac
     1201 attcctgtaa aatgcaataa aatagagttt tgtttaacca ggtctcaaaa aggctcgtaa
     1261 agcatctcaa ttctctaaac gttaatatta tttatatatc aatgtttcaa ggcacttttc
     1321 aattgtttga aaagtgtctt tttcttgttt aagaacacat ttaagaacac atactaatca
     1381 catttattca atcctctctg aaaggcttca aaaacagttt tatctactac atctggtgta
     1441 tttacgtata ttctcgttgt agaaatatct gagtgtgtta aagcactaga tatttgattc
     1501 atatttgctc cgccttgtct agctatagtt gcataggtat gacgaagctt atgtggagat
     1561 aaatgagcca attcaggatg tcgtctttta attgaattga ttcgatagtt taggtaatca
     1621 gcgtgaacag ggcaatttac ttctccggat ggtttagtat aggtaaagag atactgatct
     1681 ggtgtttgat taatttttaa tctcaacaat tgctcagctt gcactgattt ccatttgctt
     1741 agtaatgcca ttacaatttc gggtacttga attacggtat cctttcgacc ttttgtaaat
     1801 ttcttttttt gatatttatc caaagcatgt ttaaggcgaa ccgtttgttt ctcaaagtcg
     1861 atatatttcc attgcaaagc atacgtctca ctttttctat caccaagata tagggtaagc
     1921 atgaaaagag tatagtcatg aagagtaagc aagtgattat tcaagtcagt gtttactgct
     1981 tctatccaag caataagctc ttctgacgat aatgcttgat taccttcccg tatccttctt
     2041 tcttctaaag caattttttt aggtgcagtg atacattgaa taattttggt agttcggtta
     2101 aattcaatat aatttagtat ttctgcaata tcaaacatag aacggacata tcctttgata
     2161 atttttatat ttgcgtattc tttcgatttt tttgttaatt catctgatat tatctcagta
     2221 tttgaattta gatagttcat tgcatactca ccaaacattg gaaggatgtg caatcggaat
     2281 atatctttag tattactaat tgttacgtaa cttggagctc tttttgatct agtagtctga
     2341 cctagttcat atctaggaag ccattttgat tcatagaatt ccttaaattt tatttttcct
     2401 tttaattcta gtgaatttgc attctcctct cttaaaactg tttcaatttt ccttttcatt
     2461 gcattttcgg ctttaatagc caattgccta gttggaaata ctttttgata cttttctgat
     2521 gtaattccag tgatttctct gaattttttt ggatacctca gattagtttt atatttccca
     2581 tttgaaagtt ttgtaggcaa aattcctcac tcccttaaaa agtaaggcag tatatttcgc
     2641 tattcttagt atatgcggcg agaagaaatt ttcaaattgt tttaaatgta ttcataaaat
     2701 ctttgatatc ctctatgtca aagcgaacta taccgtcaat tctgattact ggaaaattat
     2761 atatacgaat ccacttatct aaagtattat tatcaattcc tagaaactca caggtttgtt
     2821 ttttattcaa gtagactctc aattttataa aatcatctcc taaaaaagat tagaattttt
     2881 gaattgcttt gggtaagcac ataggaattt cacctctccc cagtttacgg cgagccttac
     2941 ggaagtatca ttatttgctg ctattgtcat ggcattttgt tttcccagaa caaaaagaaa
     3001 tctctaagta tttgtcgcta atcaggtcat ggcgtacttg agtttctagc tcgaatagca
     3061 gggatcgtta ttcaaaaatt gtatttaatt gtcaatgatc aaaaataggt tttttcctat
     3121 tcactattaa taacgataaa catgtataaa aagacacact acgaaaaaag ttaaatggag
     3181 tttactgatt ttcatatttg tatatggaac agacatagtc ttgaataatt tttataaatg
     3241 ttttgatgct tatcaactta ttcaacccgc tatccacttt aagtataatt tttccatacg
     3301 ccaataaagt agttttagga gcatgttctc atttgagaac gctccttttt tatatatttt
     3361 gaaagctact tactatacgc gtagtttcaa atagttttgt cattctaatc ttcagcaaca
     3421 gaaaaaaatc taaattataa aatttagggt tgactaaaat ataatttttg tataccatat
     3481 gatcgtgata atcattatca tttagaagga gaaagttatg ttagaagtga aaaagcttaa
     3541 tgtttgttat aacgattttt tagcattgaa cgagatttct ttaaaaatac aagaaggtgc
     3601 tttaacaggt attattggtc caaatggtgc tggaaaatct acattgctta aagctatgtt
     3661 aaatattatc cctcatcagg gagaggtctc cattaataat gaagatatca ataaaaaact
     3721 ggtgaagatt gcatatgtag agcaaaaagc tgatattgat tttacttttc caataaaagt
     3781 aaaagaatgt gtatctatgg gaacatatgc cggaatgaaa gtttttcaaa gaattaaaaa
     3841 tgcagaatgg caaagagttt caaaggcttt ggaaaaggtc gatatgggaa aatattctaa
     3901 tcatcaaata ggagaattat ctggaggaca atttcaacgt gtacttttag caagatgtct
     3961 tgctcaaaat gcggatttca tatttttaga tgaaccattt gtagggatag acttagttag
     4021 tgaacgaata attatggata cgttaaaaga attgaaacat cagggaaaaa cagtgctaat
     4081 tgtgcatcat gatttaagca aggtaaagaa gtattttgat aatattattc ttttgaatcg
     4141 aaatctaatt gctcacggaa gtgtagaatc tgtatttaat gaagagaatt taaaaaaagc
     4201 ctatggagat actatattta tt
//
LOCUS       DAFEPQ010000126         4218 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.133, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000126
VERSION     DAFEPQ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.133"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            10..210
                     /locus_tag="LP543_002655"
     CDS             10..210
                     /locus_tag="LP543_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBL2322776.1"
                     /translation="MALGRMSELGYSMMFGEVEKNFFMKHIKGRGYVDTGGSVISEFY
                     TPLVPKGYDFLDSISKLEKMND"
     gene            288..962
                     /locus_tag="LP543_002656"
     CDS             288..962
                     /locus_tag="LP543_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBL2322777.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            955..1119
                     /locus_tag="LP543_002657"
     CDS             955..1119
                     /locus_tag="LP543_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322778.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            1195..2337
                     /gene="dcm"
                     /locus_tag="LP543_002658"
                     /pseudo
     CDS             1195..2337
                     /gene="dcm"
                     /locus_tag="LP543_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            2327..2617
                     /locus_tag="LP543_002659"
     CDS             2327..2617
                     /locus_tag="LP543_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322779.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            2876..4060
                     /locus_tag="LP543_002660"
     CDS             2876..4060
                     /locus_tag="LP543_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL2322780.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            4057..4194
                     /locus_tag="LP543_002661"
     CDS             4057..4194
                     /locus_tag="LP543_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBL2322781.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         1364 a          692 c          979 g         1183 t
ORIGIN      
        1 aactttcgcg tggctttggg tcgtatgagt gaactcggat attccatgat gtttggggag
       61 gtcgaaaaaa acttctttat gaagcacatc aaaggtcgag gttatgtgga tacaggtggc
      121 agtgtgatta gtgagtttta tacgccactt gtaccaaaag ggtatgattt cttagattca
      181 attagtaaat tagaaaagat gaatgattga gtgatcttca atactgactt tatacttcaa
      241 gtaacgtata atacttttat atagtaggaa gaaaggggat aattaatatg gaaagtaatg
      301 attcttattt agagtatgca aaagcaaata aggataagtt tatttcttat attatcaaag
      361 ggaaatcccc cgaagttgga cgagacgcag tttttatggc agggagtcct ggggctggaa
      421 aatcagaggt tgccattgga ttagctgaga attatgataa ccatgtgatt attgatgcgg
      481 attactttcg cacccagttt cctgattata atggaaaaaa ctctagtctc tttcaaaaag
      541 catcttcttg gttggtagag caggcattta gatatgtctt agatcatggc tattctttta
      601 ttctggatgc aacttttgca atattaagtg ctgaaaaaaa tatctcgcgg gcgttgaaaa
      661 aacattttcg agtgacgatt ttctatgttt atcaagaccc taaaatcgct tggaaattta
      721 cacaagaacg tgaaattgca gagggacgtc atgtgccgaa aaaggcattt attaacgctt
      781 ttttcaaatc aagagagaac attgcaaaag taaagagtaa acatccagat gtattgcttc
      841 atatcataat aaaagattat acgaataata tttccgaagt tcattatgct acggataatc
      901 tagaattatt tttaccacta gggtatagta ggaaagattt ggaggttcaa ttacatgact
      961 gagaaagaac aagtaacgaa aattgtaaag aaatacaata agagcattgc ggatttatcg
     1021 gaaaatgcta ctgcaaaaga gttcaaaacg gtgattaaat atgtagccga tcaggccaat
     1081 gaaaaacaac ggaaattagt tggattaaat aaaaaatagt aggacaaatt aataatgtat
     1141 gagccaagag caaaatgttc ttggcttttt tctttgcaat gaatgaggta atgtatgaga
     1201 tttttagatt tattcgccgg tattggtggc tttcgtttag gtatggaaca ggcgagccat
     1261 cattgtatcg gtttttgtga aattgataaa tttgctagac gtagttataa agcaattcat
     1321 gatacaagta aggaggtgga aatgcatgac atcacaagtg tatcagatga gtttattcaa
     1381 tccctcggac cagtggacat cctttgcggc ggatttccgt gccaagcttt ttcaattgcg
     1441 ggaaagcggc aaagattttc cgatactcga ggtactttat tctttgaaat cgctcggttc
     1501 accactcttc tccgacctaa gcttttattc cttgagaacg tccgaggatt gctcaatcac
     1561 gaaggagggg ctacgttcga gacgatcctc cgaacgctgg atggattggg gtatgacgtg
     1621 gagtggtagg tgcttaactc aaaggcctac attccccaaa gccgggagcg gatattcctt
     1681 atcggacatt ctcgagacgc atgtaccgaa caagtatttc ctatcattgg accgtcttca
     1741 acatctgatc aaaacatcag aaatttggta aatatcaatc cttccaatcg aggaatgggt
     1801 ggacaagtct atggttcaga tggcgtagcg ccaaccttaa caggtgatga aggaatcaaa
     1861 atcgcactgc cagcaaggga tggaatctct gtagcaggac tattaccggg aaagtttgaa
     1921 caaggtaatc gagtttatga agttactgga accgccccca ccttgtcaac gaaacaaggt
     1981 ggtacaaaaa tcatgattcg aaaaaatgaa acagaatatc aagaggtgaa gccagaagac
     2041 agtgtgaatc tggcttttcc taattccaca agtcgccgag gtcgtctggg aaaacagagt
     2101 gttcatacct tactaacagg agaccaacaa gcgatcgtca cggatcagta tcaaattcgg
     2161 aagctaacac ctagagagtg ttggcgactt cagggctttc ccgattgggc ttttgatcga
     2221 gattctcaag tcaattcaga tagtcagttg tataagcaag ccggaaactc tgtaacagtt
     2281 ccggtcattt ttgatattgc cagaagatta aaagagttgg atgtacatgg tttatgatga
     2341 gttgattgga gaaatttatt gggtgattgg aaagattcag tcagacccag aattagaaga
     2401 acaattgcga cggttaaaat ttgatattcg caagaatggt gtcaaagtcg ctggcgatcc
     2461 atatttaatg aatgaggaga cggatgctcg aattgaaata aatcaagtaa ttgcagaatt
     2521 tgagcggatt gcggaccaag caaaagaacc agatattcgt caatacttgt tagaaatgaa
     2581 agcagagttg gaagtaaatg gtattacaga tgagtaacct aaaccaacac aactgactgt
     2641 taggcgctga ccgcggagga ttcgcgaagc agaaatcctc cgtggtgctg tgcctgttca
     2701 tggacggcgt tagccgacca tgatgtctca cccccgtatc taacaggggg gtacaaaaac
     2761 acaaaaaata agtaagcaac cattaagaat gcttgttgta tcaaagatag taagtaattt
     2821 gtataggtgt taactcattt gaaaaagtta acaccttgaa tttggaggtg agaacttggc
     2881 acaacggaat ttagattatc ggctattaaa agaccgacgc aacgaatacg gcgtttcaca
     2941 aaataaattg gctacggctt gtggacttag tcggccgtac ttaaatcaga tagaaaatgg
     3001 tggcgtaacc gcatctataa aaaccatgag gaaaattttt gaccaactgg aaagctttaa
     3061 tcccgatttg cctttgacgt tactgtttga ctatgtaagg attcgctttc ccacaacgga
     3121 tgcacgaaaa attattcaag aaattctcca tttgaaattt gagtatatgc tccatgaaga
     3181 ttatgccttt tactcctatc aagaacagta tgtcatgggt gatattgtgg tgatgttgtc
     3241 tcatgaagaa gataaaggtg tgcttttaga attaaagggg cgaggttgcc gacaatttga
     3301 aactttttta ctcgctcaaa agcgtagctg gtacgacttt ttcgaggatt gtctaaaggc
     3361 cggtggcgtg atgaaacgct tggacttggc aatcaatgat cgagtggggc tattggatat
     3421 acctgagcta acgaaaaaat gtcagaagga agaatgtatc tccttgtttc gtacctttaa
     3481 aagttatcgt tccggggaac ttttaaaggc tgatgaaaag gacggcatgg gcaatacctt
     3541 atatatcggc agtctcaaga gtgaggttta tttctgtttg tatgaaaaag actatgaaca
     3601 atatatcaag ttagggattc cattagacca aactgagacg aaaaatcgtt ttgagattcg
     3661 actgaaaaat gaccgtgctt ttcatgcgat tcaagatttg ttaaaaggtc gtagcattga
     3721 gagcaccacg ttttccatta tcaatcgtta cttgcgattt gcggataaag tagaaggtaa
     3781 aaggcgaacc aactggccct tgaatgaaca atgggggcgt tttattggac ggaatcggaa
     3841 agagattcaa ttaacttccg agccaaaacc gtataccatt gaacgaacct tgaattggct
     3901 aggccgacaa gtcgccccta cgtggaaaat ggctaaggag ttggatcgat tgaatcggac
     3961 aacctatatc caggatatgg tacagaatgc acgcttatca gaccgacata aaaagatttt
     4021 ggagcaacaa agtatggcaa ttgaaaattt gattatatga aaagaggaat cgtcatggga
     4081 attgtaaaag atattttttt ggtatttggt ggcagtatgc ttggtgttac tgtgatgtgc
     4141 ttgatgaatg caagtgcagc agcggatcga gcaatggaaa agaaaggaaa gtgacaagat
     4201 gaatttcgga caaaattt
//
LOCUS       DAFEPQ010000127         4216 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.134, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000127
VERSION     DAFEPQ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.134"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            624..3263
                     /locus_tag="LP543_002662"
     CDS             624..3263
                     /locus_tag="LP543_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL2322782.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3431..4129)
                     /locus_tag="LP543_002663"
     CDS             complement(3431..4129)
                     /locus_tag="LP543_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBL2322783.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIAAILLIMIPI
                     ILFATTIYDGSASVSDATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVIGLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
BASE COUNT         1278 a          805 c          911 g         1222 t
ORIGIN      
        1 ttctttctgt tctgtccaaa ttattctaag atttctcttt accaacatca tttgacaaag
       61 agccagaaaa agccgagctc tcgataagct cggctttctt gtacctcgga aggattcaaa
      121 taaacagaaa tgattgtaac tttccatcat acatgcaaag cacggtgttg aatttcttca
      181 tgttaaacaa aaattttgtt ttcgttacat cttcaacatt tgaaacatct cttgttccta
      241 ttcaattttc agacgtttac gttattcaat tgttacgtat cgcattactt cttgtttcgg
      301 atacttcgtg atccaaacat ccatacgcat tcactattta taggtttgta ctctacacgc
      361 atgtctgggt gaaacctgta cttctaacaa ctttatttgc ttacactgtt ctatcgagaa
      421 attcttctgt ctatttgatg gtatcctcct tccttacatc tatataatag catcctttgg
      481 tagcgtttgc aaatataagc actttaaatt ttacgctttc ttaacaaata atcgataagc
      541 cctttatttg aagtaaataa aaaaaggatg ttatagtaaa ataagtacct ttttttgtac
      601 gaagatttta aaggaggaat aggatggaag aatacaagca gacaatagaa gaagtaaaga
      661 aggtaacaga agcagatcca gatactgggt tgtcttcaca agaagtggat gaacggcgaa
      721 aaaagcaggg attgaataaa ttcgatgagg cgccgaaaga atcgatgatc aaaaaattct
      781 ttcgaagctt atccgatttt acaacgatca tccttcttgt agcagcggtg atttcctttt
      841 ataccgcatt tgctacagaa catggggatt tatttgaagg actgctgatc atcgctatcg
      901 ttgtgatcaa ctcggtattg gcgattgtcc aagaaggaaa cgcagaaaaa gcactggaat
      961 ccttgcaaga tatgaataaa caaacggcaa ctgtcatacg agacggcaaa gtgaccactg
     1021 ttgagtcaga gcaactggta gtgggtgata ttcttgtttt agagtccggt gatgctattt
     1081 ctgccgatgc ccgtttgatc gaagcatcac aattacgtgt agaagaatcg gcgttgactg
     1141 gagaaagtga agcagttgaa aaagatgcag catttattgc tgaagaagat gagtcattag
     1201 gtgaccaact taatatggta tttaaaggat gtacggttgc tgcaggtcgt gggaaagcga
     1261 ttgtcactgc cattggtatg gctacagaaa tgggtaagat cgcggatctt ttgaacgaaa
     1321 atactatgca aaaaacacct ttacaggtcc gattgaatca gctaggcaaa cgaatcagta
     1381 tgatcgcttt agcggctgcg gctttagtat ttgtgattgg tgaactgcaa ggagaaccac
     1441 tccttgagat gttcatgact tcgatttcct tggctgttgc agctgtaccg gaaaccttga
     1501 ctgttattgt tacgttgaca cttgcatacg gtgtgcagaa gatggcgagg aaacatgcga
     1561 tcatccgaca gttgccagca gttgaaacgc taggaaccgc taatgttatc tgctcagaca
     1621 aaacaggaac gctgacacaa aatgaaatgc gcgtccgccg ggtatggagc aaagaagatg
     1681 aagtaacgaa tatcgaagat tcaatgacga acagcgcgat ggaaatattg aaaatggctg
     1741 cactatgtac agatgtgacg gtcgagcaag aagatgacga ccttgttatc aaaggtaatc
     1801 caacagaagt agccatcgta agggctgttg aagagaatta tcatacaaaa gcagaattag
     1861 aagaaaaata tccacgagta aacgagcttc cttttgattc tgaacgaaaa atgatgacaa
     1921 cagttcacca aatgggcgaa aaatacatct caatcaccaa aggagcattt gatgttcttg
     1981 ctccgcgctt tagttcaggc gatgtagaac aagcggcgat cgttaatgat cgctttggta
     2041 aacgggcact gagagtgatt gctgttggtt atgctactta tgacgaagaa ccgcaagata
     2101 tttcttctga agcactggaa aaagacttac gtttgattgg tctgatcgga atgatcgatc
     2161 caccacgtcc agaaagtaaa ggagcaatca agcgagcgaa aaaggctggc atcaaaacag
     2221 tcatgatcac tggagatcat gtggtaactg ccagtgccat agcgaaagaa ttaggtatct
     2281 tgaatgatcc gtcagaagca ctctcaggat cagaactgca tcaattatca gacgaagaat
     2341 tagatgcgcg tgtcaaagct ctctctgtct atgctcgggt aacgccagaa gacaagatcc
     2401 ggatcgtcaa atcttggcaa cgtacgggtg cagtcgtagc gatgactggg gatggtgtga
     2461 atgatgcacc agcgttgaaa gcaagtaacg ttgggtgtgc catggggatc acagggacag
     2521 atgtggcgca aagtgccgca gatatgattt tgacggatga caactttgcg acgatcgtag
     2581 atgctgttag tcaagggcgt tcagtttatc agaatattcg taaagcaatc aactttttac
     2641 taagctgtaa tatttcggag atctttattg ttttgattgc gatgctgtta ggatggggag
     2701 caccatttac agctgtgcaa ctactgtttg tcaatgtagt tgctgatgga cttccaggat
     2761 ttgctttagg aagagagcca gcggaacacg gcatcatgga tcagccaccc attccgaaaa
     2821 atgaagggat ctttgctcga ggtttattgc aaaaaattgc cataaatgca ggggtcttca
     2881 ccatcgtaac cttgtttggt tattatttag gtagttatgt cgatactatc agcccatggg
     2941 tagacgcaag tcagcatgtc ggtcaaactg ttgcattctt gatcttagct tattcatcga
     3001 ttctgcatgt gttcaatgta cgcagcagcc aatcgatctt caaagtcaat ttagcaacca
     3061 ataaagcttt ggtagaaatg gcgttactgg cattagctat tacaacggct gttgctttgc
     3121 tgccatttac gcaagaacta ttcggcctag tacacatcag tttgaatcac tggttcttgg
     3181 tagggatttt atctatcgta ccaatcgcgg tgaatgaact gatcaagttc catcgattac
     3241 cggaagcaga agaagaggaa tgattaactg tttctaagtt gattttcttt ttactggaac
     3301 tatacaaaaa ggaggtgtga catgaatgtt gtcacacctc ctttttgcta atgggctgaa
     3361 tgtttctttt actgcctctc gataaacggt gttctcctac caacccctcg gtcacaagtc
     3421 acactctctt ttaaatatct tttggcaatt gttcataaaa tgctgctttt gttgcggaaa
     3481 tgtatgggct gagccataga taaccaatac cagcagttgc taatgctaag atatgccagc
     3541 cgataaatga aagatctaac cagaaaagtt gtcctttata accatccatc aattttcgac
     3601 ttgcagtgat cgtatccagt actttaggtg tttgtcctgt ttcttggacg atatcgtagt
     3661 agataaaata gctttgagaa taagaatagc ttttaatgat tccaggaata actaatagca
     3721 aaccccataa tgttgtaaag atcgagatca gcacagctaa gccgatcact ccgccgataa
     3781 acaaaccttt gaatccacga aatgcatctt tgaatggttc gatctgatct ttttgtccac
     3841 gtaaaatatc aagataagtc caagaaatac cgctcatgaa caaagcgcta atgatgctgg
     3901 aaataattcc gccaccgcct cctgaactac cgcctcctga agttgtcgca tctgagacgc
     3961 tcgctgatcc atcatatatg gtggtggcaa ataagataat aggaatcata ataagaagaa
     4021 tagcagcaat accaatcaat gtagggatta gatttagtaa aacagcttgt ccccaacgtc
     4081 cttttagact ctcttttgct tggcgtttta attctgcaga agattgcatg atttttccct
     4141 ccttttcttt aataacacca atataccatg tatcaaaaaa tatttctgtc ataatgtttt
     4201 tctctttttt gttatt
//
LOCUS       DAFEPQ010000128         4195 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.135, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000128
VERSION     DAFEPQ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.135"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(2..1222)
                     /locus_tag="LP543_002664"
     CDS             complement(2..1222)
                     /locus_tag="LP543_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBL2322784.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1242..1853)
                     /locus_tag="LP543_002665"
     CDS             complement(1242..1853)
                     /locus_tag="LP543_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBL2322785.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1896..2828)
                     /locus_tag="LP543_002666"
     CDS             complement(1896..2828)
                     /locus_tag="LP543_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBL2322786.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLSNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3195..4109)
                     /locus_tag="LP543_002667"
     CDS             complement(3195..4109)
                     /locus_tag="LP543_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2322787.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLINEGKNTYFCYANFEKIHQR"
BASE COUNT         1190 a          849 c          691 g         1465 t
ORIGIN      
        1 tttattttac agaagcactc gaaactgcat ctactgcagt ttcatcgtgt gtgctggcgc
       61 tggttactgc atctgttttt ccaccatgaa cagatgcact tgaaaccgca tcttcatttc
      121 caacaagttg ttgacccgtt tgttgtaaat accactcaat gattggtttg aaatcaagaa
      181 tgtattcatt gattccagca tactctccat tttcatcatg cattgcttgg taattatgca
      241 caatatattt ttctggcact tgttttggta catgaacacg taccgcatct gttgctccgc
      301 ttcgcagttg ttgaattacc cactctacat ttttatacga tttttctggg tgacagttgg
      361 ctaatggatt ccctacctga ccgggcacac gagcagctaa catctctttc gcttcttggc
      421 gcaaattata atataaaaat tggttattat tatctgcaaa agttaattcc atcggcatcg
      481 aatctaaaaa gtaattgatt tgatccactg tcaataaccc acgatttaat ttgacgtaag
      541 tatttccaga aacggcaccg actgcttcag aagctttttc caaccaatga tcatctgaca
      601 tatcaacatc gggtattgtc gtagcaattg ttcctgtcgc atgcaaatct tctggtggaa
      661 gctttttagg ccctgctaag gtagacacga ctttaatgaa ttttacaaaa cttgctaatg
      721 tactgcctaa aaagtcaaca gttcgtggat caatcaagcg atcttcttgg tcaaatgctt
      781 cttttgcttt tcccaataaa aattcatttc cgggcatcac taatgcattc acgcctggtg
      841 cgtccaaaat ttggcgcaag tgtaattgcg cgcgtgaagt tccttgatcg tagtaagaag
      901 cacccacaac cattacaggt ttattttcaa gtggatggat tttaaatgat agccattcaa
      961 gtgcactttt caatcctgct ggaaccgtat gattgtgttc tggcgtagca ataattaccc
     1021 catcagccat taagattttg cgatttagct gttgaatagc aggactattt gtttgatcat
     1081 catcttgatt gaacagaggt aaatctttga tttcaatgat ttctaaatta aatagttcat
     1141 gatattgttt ttggataaat gttaataatt tacggttata tgaaagttca gctgctgatc
     1201 cgacgatacc tattaatttc atctttgttt gctcccctcg attaaatcgt ttcccatgaa
     1261 aagctttcgg cttcacgttt atttgcttgg tgcgcatgtt gcaattgact agtgattgtg
     1321 acaaagagta agaaatcttt gaatagttca cgtaattttg cgacttgttc ttcatcaatg
     1381 agtcgtcctt gctcatcaaa ggcttggagt gaatgaccca ataaaaattc tgagcttggc
     1441 ataattcgtg cctttaactc tggtgaatcc aatatttggc gcaaatgtgc ttgtgcgcgt
     1501 gaagaaccta acgtgccata agacgctccg gtaatcatca ctggtttatc cacaaacgga
     1561 aaaataccat atgaaagcca atttaatgcg ttaatcaatg aagcaggaac cgcatggtca
     1621 tactctggtg tgctgatgat tacgccatct gcttgttcaa tttttccagc aatttcttta
     1681 acaacatttg gtagttctgt gttttcaggt ttgttaaata gtggcagctc cttgatttcg
     1741 acaatttcga tttctgcctc atttacaaaa taatttttca taaactgtaa taattcacgg
     1801 tttgttgacc gcgttgaatt tgttcctact aatccaataa atttcatctt cattctcttt
     1861 cctttctaac atcgatttct gactgttcgc acgtactaaa ttttttcaaa tccttttgta
     1921 tacaagaatt gattctcttt tgttacaact gctccctcga tacccggctg ttcttcaagc
     1981 gctttcaaca cttctaaaga agatgctcca aataaacgtg tagtccagat ttctccatct
     2041 acagaagaat cagacaaaat cgtcaggctt gctacgtcat ttttaactgg aaagccagtt
     2101 ttcggatcaa tgatgtgatg atacactgac gaaccatttt gtagataacg ttcgtagatt
     2161 ccagaggtaa ctaaagattg gttgcttagt ttaaccaccg caagcgactc ttcacgtgaa
     2221 cctttaggat ttcgcaaacc aatacgccaa gcaccatccg aacgtcttgg tgattcacca
     2281 aaaacaacga tatttcctcc taagttaact aaagcaaacg ctgcatttcg ctctcggcaa
     2341 tgtgtaatta atttatctgc aatgtaacct tttgctaacg cccccaagtc aatcatcatt
     2401 cctggtttgg ttaaaaaaac ggttttcatt ttttcatcta attgaatttg gtgtggatcc
     2461 gttagcagaa gtaactcagt tatctctgct tttgttggaa ccaatgcatc ttcaaatcca
     2521 atgtgccatt tttgtacaac cggaccaata gcaatattca gataactatt tcttgcacaa
     2581 ctatgtgctt ttcctaattt gatgagctca aaaagttcag gatgtacaat cacagcttgt
     2641 cgtccagcag cttgattgat ggccattaat tcagaagttg catcatgtgc gctgaagcga
     2701 tgttcataaa cgtgcaacca ttcgcctaac tctgctagta atcgactagg ctcttcatgc
     2761 ccaacttgta atgtaattgt tgtccccatc atttgccaga ttttcgtttc ttcgatcacc
     2821 tgattcattt tgtaccttct ttacttgaac tacggattac atgagtaatt agtttttatt
     2881 tttgttactg ttttcacaac cagatgatac gcctgctaga tttatttgtc aaccgatttt
     2941 atatattttt ttatataata aggtaatttt aatataaaat tttatattgt aaaaaaacaa
     3001 ataagggaaa caaacaacac atttttgtgt gttgcttatt tccctctaac tatcgttttc
     3061 tatttgatgg ttaacctact taaaataaat actgcaacga caaacggaga tcaagcatgc
     3121 tttgtaacgt attttacgag aattacgact ttattttatt actctatctt gctgccatac
     3181 ccgtctatcc ctaatcatct ttggtgaatt ttttcaaagt tagcataaca aaaataagta
     3241 ttttttcctt cgttaatcaa tagtatcatt tttttcatat gatttaattc atcaattaat
     3301 tttttgtttt tattaatttt agcaaaagct agtgcatctt gaatcaattc gagcacttct
     3361 gccttcgact tatttttagc ctgtgcgttc ttcgctaatt gggcagcagc tcgtgctaag
     3421 taataagtca aatggttatc cgaacaaata tcgattgcgt aatttagtag tgcatcactt
     3481 gtttccaaat caccgtcttg cgcaaaaaaa acaccacaat gaaagacgat atttaacacc
     3541 cgccagatat cttctgttgt acgaatcgga tacgtataaa ttttttcaaa cactcgattg
     3601 aaataaactt ctgctttttc caactctttt tctctagcat agaccattcc aatacctgta
     3661 tatgccaata aacgaaacaa cgtgttggct gtttctgcca tcaacaactg atcaaaatgg
     3721 aacaacacat ccgtaattgg tgcattcgta aaaatcattt caaatccttt tagatagtag
     3781 taacgtgcaa tcagctcaga ctcgtcaatt accttgggat caatggctgc taaaatctgt
     3841 gtcaattgtt catattcact cgtaatcagt gaaaactctg ctttttctag ctgttcatca
     3901 agttgtgtat tgatcacgtt catcgaagaa aaaagttcaa ctaacggaag attgattcga
     3961 ttacataatt tgattaaaat ttttaacgaa gggacttgac cattgttctc aaatttactt
     4021 aatgttgctt gtgtacaaat tccttcagac acttcttttt gagataagtt ggcagccttt
     4081 cttgccgtaa taaatttttc aatgttcatt attttctctc caatcagacc tcacacgaaa
     4141 atcatttctg tctcgatttt agcatgtttt ctttgggaat gctttaattg gttaa
//
LOCUS       DAFEPQ010000129         4093 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.136, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000129
VERSION     DAFEPQ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.136"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            114..1103
                     /locus_tag="LP543_002668"
     CDS             114..1103
                     /locus_tag="LP543_002668"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBL2322788.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(1420..1944)
                     /locus_tag="LP543_002669"
     CDS             complement(1420..1944)
                     /locus_tag="LP543_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HBL2322789.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            2231..4042
                     /gene="scm"
                     /locus_tag="LP543_002670"
     CDS             2231..4042
                     /gene="scm"
                     /locus_tag="LP543_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBL2322790.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         1341 a          864 c          751 g         1137 t
ORIGIN      
        1 aatgaacgcc caccctcttc ttttttctct ttccttcttt cgtttttgct ttatgtatca
       61 accgttgttt cttcgaaaac tattttgttc tctccttctt cctgatagaa tggatgggaa
      121 aggagcttga tgccatgaca acgaaagaca aggtacttca attattgaaa aagacaccag
      181 attttctttc cggagaaaag ttggcacagc aactggaagt ttcccggaca agtatttgga
      241 aagccatcaa agaattggaa aaggcaggct atcgttttga gcacgggcct acagggtatc
      301 gttacctgcc ctctgacgta ctagatgcaa aagaaatcca gcaagagcta cggtcgttta
      361 ttcctgattt gaaagtacaa acgatgttga cttctgaatc tacgatgaaa gatgccaaac
      421 tggcagcagc tcaaggagat tctgtttcga agttatttgt tgctgataca caagaacaag
      481 ctcgtggacg ttttggacgg ccttttttcg ctgaacctgg acgaggaatc tacatgagtc
      541 tcttgctcca tccagacaaa cggtttgatg aattgcctca atatacgatc atcgcagcca
      601 gtgcctgtgt gaaagccatt gaatctgtaa ccggtcgttc tgcttcgatc aaatgggtca
      661 atgatatcta catggatgga aaaaaagtct gtgggatttt atctgaagca gtcagtgata
      721 tggaaagcgg caggatcagt catatcatca ttgggatcgg gcttaatttt tctgttcctc
      781 aaaatcattt tccagctgat ctccaagaaa aagccatctc tgtctttccc gacggacaag
      841 ctagtgtcac aaggaatcag ctaatcagca aaatctggca agaattgttc acttatcttt
      901 ctcacccaga aaaagcgttg gacttctata aagaaaaatc attcgtattg ggaaaaaccg
      961 tgtcgttcaa tcaaaaagga acgacttatg tcggtcttgc aaaagaaatc ggtgatcatg
     1021 gcgaactgat cgtccaaaca aatgaacaga agatgagttt gtcttctggc gaaatcagtc
     1081 tcacatctat ccgtacgcaa taattatcag atttcacaaa aggacacctg atctcataca
     1141 gtgtcctttt ttctgaattc tcttcaaaag actctcctta ttctatcagc tgacagtttc
     1201 tctatcctct agaaaaaggt attctaaaaa tatctataaa gcatggtaca ctttctttaa
     1261 atgaatcaat gcaaaaaaca agagagtcat cagtttacac gatcaaaaga gatagcaggc
     1321 cgcttccttt ttcaatctta tagatagacc atgggtaacg ataaaaacaa aaaagaactt
     1381 gcgacttatt ttgccgtaag ttctttttta gctagtgcct taatctctta atcccaacaa
     1441 acgtctcgca tagatcacct gaccagagtg catagcagca tcatcaataa tagaaacgag
     1501 acgcattcct cgtgtgactg gtggtgtcca gttctcgtca attacttcat ctagctcgga
     1561 aggttccagc cttcttaagt aagaaatagc aaaatcagca gctcctcgca agtatcctag
     1621 cagatcttct atttgattag cttggatctg ctttgcttgt tccagactat gcttccaatc
     1681 ttgttcgtta tctgctatcg tcatcggaaa ccggctttcc cagtcttgac tatgccaaat
     1741 tggagaagtt cctgctaaat cagcaatctg gaaatctaat tctcgagcgg tatgccaagc
     1801 cagccaagtc attgatttga tctgcggggc tctatcttcc actggaaaac gattggcctc
     1861 ctcaatagag acgccttcaa acaaacgttc aaaccgatcg tatgcccgat ccacactctc
     1921 gcatagcata tctaaataat ccatttattt tcctcctttt tatttatcct acttactttt
     1981 tcctaattct aggataccta cttatatgtt actacacacc cttatttttt acactttaac
     2041 agtcaccatc atcaatagtt tggagattga aagaaaaaga tagtgtcgaa ctatcaattg
     2101 aacaagaagc aacaatatga acgtttcatt aatattcttt aaataaacaa tgatttttca
     2161 aacgaacagt ttaagactag gagaaaaaga gtataatagg aaaatgtttg aaaggaaagg
     2221 aaggggaaaa atgaaaaaaa gggcaagttt actagtccta gttgctttgt tgttcaatat
     2281 cttttcgtat ggtgtggccg tttcagcgga aacatacatt cctaaagacc ctgggaatat
     2341 ttataatgat ctaactggta actatggctt gttaggaatt gctagtcaat tccacgtatt
     2401 tgctaaaaat aagacaactg tgaatgcaca tacagacgga aatatagcaa cacatgaact
     2461 tgatgcaaaa aataattttg gtacagagct tcatgaaggt ttattgaacc aagaaataaa
     2521 ttatatacaa aaaactgatt ctttcgctaa ttcttcaggc atacctacag gtagcacaag
     2581 aatgaataaa tttgtcgttg gcagtaacgt ggatgtagca cttcaagatg gtcatcctac
     2641 agtcaatgga tccaggcttg atcacttaac tccagatgat ttttataaag atcgtgctgg
     2701 gcatgaatat attgatttcg agcaggaatt tgctaaattg aatgtagcag caaatgatct
     2761 tgcaacgatc actccagcta aaacatatac tgctgcagat tttccagaca tgaacaatcg
     2821 gacaatcgat ttaactgatt tatcagatac cgggttttta ttggtaaata tcgatgcaga
     2881 agtgttaacg atgaatacgc cacttcaaat cattaatcca aatgatcaag tagttgtatt
     2941 taatgtcatc aacagttctt ctgcattgaa cgtacagtca ccaatcaaat acaatgatcg
     3001 ttcaaatcat gaaacagaag atttttcgga tgcgaatatc tcttggaatt ttggaaatga
     3061 gatgactgat ttgacaatca gtgctccttt tcaaggaaca atccttgcgc ctaatgctac
     3121 tatccgcatt actcaaaacc aagacggaac gattatagga acaaatgtga ttttagatgc
     3181 agctaccaat cgttgggatc ctaacgaaat atttatcagc gacaatggta cagatacgac
     3241 agacacaagc gacagtacag atacatctac cacaagtgat agctcagatt catcaacttc
     3301 aagcgacagt acagatacta cctctaccac aagtgatagc acggatacat cagcttcaag
     3361 cgacagtaca gatactacct ctaccacaag tgatagcacg gacacatcag cttcaagtga
     3421 cagtacagat actacctcta ccacaagtga cagcacggac acgtcagctt caagcgacag
     3481 tacagatact acctctacca caagtgatag cacggacaca tcagcttcaa gtgacagtac
     3541 agatactacc tctaccacaa gtgatagcac ggacacgtca gcttcaagcg acagtacgga
     3601 tacatctacc acaacaagtg atagcacgga cacgtcagct tcaagtgaca gtacagatac
     3661 tacctctacc acaagtgata gctcagattc atctaccact acaagtgata gcacggacac
     3721 gtcagcttca agtgacagta cagatacatc ctctaccaca agtgatagct cagattcatc
     3781 aacttcaagc gatagtacag acacgtcgtc taccacaagc gatacacgtc atgcaagtgt
     3841 ctccttctca aaaacaagtt cggataacag gacgaacttt aacttaacta ataaaacaag
     3901 caatgacaat ggaaacaaat caaaaagagc acttcctaaa actggaagtc aatcgaataa
     3961 ttggataaca ctcgcaggcg ttatattact tgtaatcggt ttaagaatca cttttatgag
     4021 ttataagaaa tcgaaaaggt aaaaaaatat tcaagataaa aaagagcaac gtcgctaacg
     4081 gacgttgctc ttt
//
LOCUS       DAFEPQ010000130         3956 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.137, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000130
VERSION     DAFEPQ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.137"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..156
                     /locus_tag="LP543_002671"
     CDS             <1..156
                     /locus_tag="LP543_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBL2322791.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="LP543_002672"
     CDS             175..1452
                     /locus_tag="LP543_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2322792.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="LP543_002673"
     CDS             1462..2118
                     /locus_tag="LP543_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2322793.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="LP543_002674"
     CDS             2118..3494
                     /locus_tag="LP543_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2322794.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3624..>3956
                     /locus_tag="LP543_002675"
     CDS             3624..>3956
                     /locus_tag="LP543_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL2322795.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1594 a          423 c          693 g         1246 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc gacatc
//
LOCUS       DAFEPQ010000131         3812 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.138, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000131
VERSION     DAFEPQ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.138"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            94..2193
                     /locus_tag="LP543_002676"
     CDS             94..2193
                     /locus_tag="LP543_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBL2322796.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2389..3252
                     /locus_tag="LP543_002677"
     CDS             2389..3252
                     /locus_tag="LP543_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBL2322797.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3357..>3812
                     /locus_tag="LP543_002678"
     CDS             3357..>3812
                     /locus_tag="LP543_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL2322798.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT         1526 a          636 c          772 g          878 t
ORIGIN      
        1 agtaaatcct aaaaataagc tattggcgtg acgtatagaa tatgatagat taaatggtag
       61 cgtaaaccta gtaaaggagc cgatcgctaa gtgatgaaaa aatgtccaaa ttgcggatcg
      121 aaaaaaatgg agcagaatac tatctgtcca gactgcggtc atcaaataac tgcagaagat
      181 cagcaaataa aagaatcaat tgaagaaaca aatacagatg cagctatgca ggataaatca
      241 actgaagatg cagtctttcc tgatacagaa ttaaacgatc caatcgaatg gtcggaattg
      301 aaagatttac ctctagaatc tgtaatggaa ttattcgaaa aagcagaacc agaagacgaa
      361 gaaccacctg ctcacgatca agtaactaat atagagaaaa atgaacagaa gaaagcaact
      421 gttcatgaga cttcggaaga atcaggaaag aatcaagaag aaacgcttga atcaaaacaa
      481 aaaagaagag tagctgaact gaaagaaatc gtcgataatg aggaagaaaa ttcgatacta
      541 tccgcttata tcaaagcaca tcgtgaagat acaaaagaag agcacgcgaa agagctgctc
      601 aaaatgatca gtgaaaaaat tgcacaggaa aacaaagtag agccagaaaa ggaacaagaa
      661 actgaaaaag actctgatag agaaaacgcc acaggtaaag aaacagtaaa aataaaagac
      721 gtaaaaacgg atatcacaga aaaaactgaa gtatttactg aaaaacaatt agaaaaggaa
      781 aaaacgaata aactgccgga agcagagaaa aaagaaatac tcgaaaacac agacaaaaag
      841 tcaacagagc ccgatgaaat aaatagtgaa tccgatgctg aaaatcaatc tgtaagcaaa
      901 gagggtactg atcttgaagc aaaaatagaa aaagaagaga accagcctaa gctgtttgaa
      961 gcagaagcga aatcaataac aatcgtgcca gatactgaaa aaacacatca agaatcaaac
     1021 ttagaaaaaa cagatccaga taaagcgcaa aagaaaaaat caaaaaaagt accttatctc
     1081 gttttagcag cagtacttct actcggtgga ggaagctggg cttattatga ccatcatcaa
     1141 aaagtagaag ccgaagtagc agcagaagca aaaagacagc aacaaatgga agaacttcat
     1201 gagaaacttg ctgcctttta tacagatgat caccatcaat ttattcgtgc agatatgatt
     1261 catcaagatt tatcaaaact aaaaaatgat ttatccagtc ttaagaatga aaaaggatat
     1321 tcagagttag aaacaactta ccaagatatc caatctaaga tccgagatat ccaaaaagtg
     1381 aatgagtggt tcatagcacc tgccatcgtc gatgatcatc tagcagatga catcaagttg
     1441 aaagcagata aagagatcca agcaatcgaa acggataaca cagattttgg aaaattgatt
     1501 caacaagcac aaacagaagc taaaaatcaa tatgctgaat tgcaaacagc taaagaaaaa
     1561 acagctatag tctttaaaga cggaaaagta gtggattcag caacacgcga ccaatacaag
     1621 acggcaaaag aagcagtaga aaaagtgaaa aatacagatc tatctgctca gttgaatgaa
     1681 caattgaaac aagtagataa gatgctcact gataaagaga aaaaagaaga agaggaaaag
     1741 aaaaaagcag aagaagcgaa aaaagcagct gaagaacaag caaaacaagc cgctcaagca
     1801 gcagctgcag ctcaagccgc tcaacaagct gcggtcgcac aagcagcaca gcaagaagcg
     1861 gcatcctcga ccccttcctc ttcaaactca gcgaatcagc caatcaaatc tgccagagca
     1921 agtgatattg ccgatgcatc taatccagca tggacgtggg caccaggtgt ccaaagcaaa
     1981 gtgattgcta catgtatcca acgtggatat atcgttgaag gaggttacag attagaaaaa
     2041 gccagaatcg aaaatgggga aggctattat aatctatatg ccacttcaac aaaatctgca
     2101 ttgatgaaag gcattggaga aagtgctttg ccattttaca tcgtaaccat caactgtaaa
     2161 actggatggt tcggaggaaa tgggagcaat taattttagg caaatgaaag aggctgtgag
     2221 cctgccgttt aacaccgttt attcgaaaaa ggggagtatg acacaactgt tgtcatactc
     2281 cttttttcac ctttccaaac caaggctcag ctattcaagt agataacatt atgttatgat
     2341 agcaaagaaa tgtattaaga aaacctttca ctatagaggg agaaaaaaat gaacgattta
     2401 ttagcaacta tatttacagg tattattatt gatgaaaatg aaaaaaacta ctttgtccaa
     2461 aaaaacggac aaacctttgg cttaagcaaa tcagaaggtg aacacaaaat cggtgaagca
     2521 gttgaaggat tcggttacca aaatcaaaaa catgaaaatc ggatcactac acaaattcct
     2581 aaaagccgta tcggtcatta cgcttttgga atcgttactg ctacacgaaa agatttaggt
     2641 gcgtttgtcg atatcggtct tccagataaa gactttgtcg tatctcttga tgaattgccg
     2701 acaatgacag agctatggcc taaaaaaggg gatcgcttga tgatttccct tagagttgat
     2761 gcaaaagacc gtatttgggg cagtttagca gatgaaaaaa tattcaaatc tttaagcaaa
     2821 tatggaacag aagaaatgaa aaacaaagat gtgataggaa cagcataccg cttgaaatta
     2881 acaggtacgt acgttttaac ggaagatttc tatcttgggt ttatccatcc ttctgaacga
     2941 tataatgaac ctcgtctagg cgagcagata aaaggtcgag tgattggtgt ccgaccagac
     3001 ggtgtactga atctttcatt gaagccaaga gcatatgaag ctatatcaga tgatgcacaa
     3061 atgattttaa ccattttaga acgttctgca gatcagcaaa tcaactttac agataaatcg
     3121 gatccagaag aaatcatgaa agcttttggt atcagcaaag gagcatttaa gcgcgcatta
     3181 ggtaatcttt tgaaacaagg attgattaca caagaaaacg gtgtgacaaa actagtcaaa
     3241 aaatctaatt gagaatccct tgcatgtttt tcttaactag cttataataa ttttaattaa
     3301 catagtgtta tatctttttt tatttgtaat tattataaat tgttggggtt gagtaaatgg
     3361 tcatgaatgc tatttcggcg atgaaaaaaa caaaacagca gctacatgaa gcggggttta
     3421 agctgacacc gcaaagagaa gctactctgc ttgtgctttt ggaaaatgag aaagagcatt
     3481 tatctgcaga agaaatcttc tttttagtga aaaaaaagag tccggaaatt ggacttgcta
     3541 ctgtttatcg aacccttgaa atcttgacag aattgaaaat tgtcgataaa gtaagtttta
     3601 atgatggatt agcccgttat gatttgagaa aagaaggcgc caaacatttc catcatcatt
     3661 tactatgctt gaactgcggc acgatcgagg aagtagaaga ggatctttta cttgaagtgg
     3721 agcaagtagt agaagaacgt tatcattttt tagtaaaaga tcatcggcta acgttccatg
     3781 gaatctgtca ggaatgctat gaaaaagaaa aa
//
LOCUS       DAFEPQ010000132         3800 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.139, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000132
VERSION     DAFEPQ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.139"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..387
                     /locus_tag="LP543_002679"
     CDS             <1..387
                     /locus_tag="LP543_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBL2322799.1"
                     /translation="NYKNEKNNKTSSSQPRKKRVYDTDSVYYILAEELFKQICQNQEI
                     KKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHNVFWASNILSAKKLREKYDTMAA
                     QANRDYKTKQTKTLEYEKFSTDELPI"
     gene            402..1253
                     /locus_tag="LP543_002680"
     CDS             402..1253
                     /locus_tag="LP543_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL2322800.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGSIA"
     gene            1250..1609
                     /locus_tag="LP543_002681"
     CDS             1250..1609
                     /locus_tag="LP543_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBL2322801.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            1624..1785
                     /locus_tag="LP543_002682"
     CDS             1624..1785
                     /locus_tag="LP543_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBL2322802.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            1782..2108
                     /locus_tag="LP543_002683"
     CDS             1782..2108
                     /locus_tag="LP543_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322803.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            2105..2920
                     /locus_tag="LP543_002684"
     CDS             2105..2920
                     /locus_tag="LP543_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HBL2322804.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            3059..3370
                     /locus_tag="LP543_002685"
     CDS             3059..3370
                     /locus_tag="LP543_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBL2322805.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            3367..3597
                     /locus_tag="LP543_002686"
     CDS             3367..3597
                     /locus_tag="LP543_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322806.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDNTFRLGS"
     gene            3604..>3800
                     /locus_tag="LP543_002687"
     CDS             3604..>3800
                     /locus_tag="LP543_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBL2322807.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEE"
BASE COUNT         1379 a          606 c          912 g          903 t
ORIGIN      
        1 aattataaga atgaaaagaa taataaaaca tcatcgtcgc aaccacgcaa aaagcgtgtt
       61 tacgacaccg actcagttta ctacattctc gcggaggagt tattcaaaca gatttgtcag
      121 aatcaggaaa tcaaaaagcc gaatctgcaa agttgggcag ataacattcg gaaaatgatc
      181 gagatcgaca agcggaccga aaaccaggta cggggaatga ttgaatggag tcagcacaat
      241 gtgttttggg catcgaatat tttatctgct aaaaaattgc gagaaaaata cgacacaatg
      301 gcagcgcaag caaatcgtga ttataaaaca aaacaaacta aaacgcttga atacgagaaa
      361 tttagcacag atgagttgcc tatttgagag gaggcgtagg catggaaact gttggggaaa
      421 tcatggaaaa gctgatacag aaagtgcttg tccaacgtgg cgaatgtcct gaatgcggac
      481 agcctttgta tggatggcgt acgaagaacc ctgatggctc agaacgttgt aagccaacct
      541 gtatgcaatg tgggtataaa gcattgcgag ttcaggaaga cttacaaaca gaacgaattt
      601 acaacgagag cctgaaagca agagcaatca attttttcaa aggtggttct gttgtgccta
      661 atcaagcgtt gtttgattgc acattgcaga attatcaaat tgtcgatcaa gaaacaagac
      721 aagcggttga agtaaccaaa cgctttgtta attcagtctt gttaggaaat ccaagtcacc
      781 ttgttttaac tggaaaacaa ggaacaggta aaagtcacct agcgatggca gcggcttggg
      841 aagtcttgaa gcgatcaaac tacgataaaa aaatcttatt tatcgggtta caggaaatgc
      901 tggatcaaat caaattttcg tacaacaatc ctgaactcag aaaaacgatt gagggatcgt
      961 tgattgcaga tatcaaaaca gcggatttag tcatcattga tgatatcggt tcagaactag
     1021 gaaaagatgc atcagatagt cgagcgtttg gcataaacac gctaaattcg ttcttggatg
     1081 cacgacagaa cctagcaacg attatcacaa cgaacttgct tggtgaagaa ctgaaaaaag
     1141 cttacggtac gagaacaata tcaagaatgt ttgtcaactc tgatggattt acgatggtat
     1201 tttctcaaac agcagacaag cgcataaaac cagtgaaagg tagtatcgca tgaataaata
     1261 ccgtaataaa aaaactgttc atcgaggtat caagtttgat tctatcgcgg aagcagagta
     1321 ttacgatcta gccttgtggc aagctgaagc gaatggttgg aaagtgaagc tccaggaaag
     1381 gtttgagctg atgccgaagt ttgaactaga gggcaagaag tatcgcaaga tcgagtatat
     1441 tcccgacttc acattttaca aaagcggcaa gctggtcaaa gtcgtagatg ttaaggggat
     1501 gcagacaaaa gactttaaga tcaaggcaaa gttgttctgt catcaatatc aagtgccgtt
     1561 gatattagcc aaaaaatatc ggaatacgtt caaggaagag cgtttttaac gaggtggtcc
     1621 atcatgacaa cagaagaagt gattcaaatg cgaattcgaa gccttcggcg tgagattgac
     1681 gatctggaac gaacaaaggc agtgatggtc aatgaaacgg ctagaaaggc aatcgatttg
     1741 cacatagaga atttaagaag ggaaatccgt agattggagg aatgagcgtg gataaggaag
     1801 cggcttggcg aaaattaatg ttgctgattc aagatgagaa ctggcaagaa gatgaagcag
     1861 tggttgctga agttcagcgt ctaggaaaga ttgctaacgg acgtatacga aaaaagccag
     1921 acaaaagaaa acagcgcaaa gggaaaatcg tcgttgtttt acacgaaggc aaaattttga
     1981 tgcaaggaac agctagtgag ctgtctgcag aaactggata tacgcgtggg actattcgaa
     2041 cgtacgcttg gcgaaatcac accgatcgaa aagggcacga atataagtat ttggaggaag
     2101 aaaaatgaac gaaaacaaat taatcaaatt gggtgtagca ggagcagtaa tcgtaggtat
     2161 tggagttatc ggaggattta agttcttcga aaaaatcgat aatggatatg tgggtgtgcg
     2221 ctattcaatg aacggcggta tcaaagatga agcactgacg caaggtgtga aatttgtagg
     2281 gattgacaaa gtgatccaat atccaattcg cttgcaaact atccaatcaa aaaatatttc
     2341 agtatctaca agcgacggca aaaagacaac gattgatatc aaatatgact acaaagttga
     2401 ttcaactaaa gcagcaaaaa tgtacaagga atttgggaat atcacttcgg aagatatcga
     2461 aagtggatgg ttaaaatcta agcttcaaaa ggtcgctcgt gaagtttatg cgaaatatag
     2521 tctgcttgat gtcctttcag gagattcctc taaagttgaa gctgaggtat taacgaactt
     2581 tgctaaatca gttgaatcta aagggtttga agtcgaagac gtaacacttg gtgttccaga
     2641 tgtcgataaa gaaacacaaa aatcaatcga tgcgatcatt cgagctggtc aagaaaatga
     2701 aaaagcgaag ctagacgcag aaactgcaaa aactcaagct gatagtgaag cttacaagaa
     2761 aacaaaagct gcagaagcag aggcagaatc taatcgcaaa gtcgccgaat cagtaacaga
     2821 caatttgatt cgttatgaag aagctcaagc tcgcaaaaag catggatggg taacagtaaa
     2881 tggagcagat actgtagtta cggatgaagc aggcaaataa tatgggattc tttatggcta
     2941 aaattctctt gttcttaggt ttagtaggag cagcatatct cgtgtatgcg ctcctttccc
     3001 aaactgatga caaagaagat gacaacaacg atgaaatgaa attttaggag gagaaataat
     3061 ggacgaacta atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcaga
     3121 tccaaaagca cagtttttga aagtagctga ggaattcggg gaaatagctt cggcgatggc
     3181 aagaagtaat gatgaactat ttaaagatag cgtaggagac gttatcgtca cgctgattat
     3241 cctttccatg caaaaaggga caaacgtaca agagtgttta gaaatggcat acaacgaaat
     3301 caaaggacgc acagggaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga
     3361 tgtgaaatga aatacgaaat accactaagt gaagcgggca ttcaagcaat tatcaatggt
     3421 cgggaggtta acatagaact tcctgatggt actgaattag tcatcagaca aagttatttg
     3481 aaagatatgg cagctccagt attaattgat cgttttaacg tgactgattc tgtggtagag
     3541 aaccacttaa aagaatttcg atcaagtata gacaacactt tcagattagg gagttgattg
     3601 acaatgaaca ccagacaccg cagagtagca aaactaagaa aacaggaact aaatgtacta
     3661 aagacaaagt ttgaaaaaga atatggaatt tcagcagaag aaacatataa agtggcaagt
     3721 cagtttgttg ctgatgcaag tgatgctatt cgtaagtttg ggatttcgat attaaatgat
     3781 gatcgtaaat gggaggaaat
//
LOCUS       DAFEPQ010000133         3709 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.140, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000133
VERSION     DAFEPQ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.140"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            170..514
                     /locus_tag="LP543_002688"
     CDS             170..514
                     /locus_tag="LP543_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBL2322808.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="LP543_002689"
                     /pseudo
     CDS             515..934
                     /locus_tag="LP543_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1026..1376)
                     /locus_tag="LP543_002690"
     CDS             complement(1026..1376)
                     /locus_tag="LP543_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322809.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="LP543_002691"
     CDS             complement(1342..2673)
                     /locus_tag="LP543_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBL2322810.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVNN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="LP543_002692"
     CDS             2929..3495
                     /locus_tag="LP543_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL2322811.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
BASE COUNT         1374 a          632 c          720 g          983 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gtaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 attgacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa
     2761 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
     2821 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggtt
//
LOCUS       DAFEPQ010000134         3602 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.141, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000134
VERSION     DAFEPQ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.141"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(175..248)
                     /locus_tag="LP543_002693"
     tRNA            complement(175..248)
                     /locus_tag="LP543_002693"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(212..214),aa:Ile,seq:cat)
     gene            complement(438..1457)
                     /locus_tag="LP543_002694"
     CDS             complement(438..1457)
                     /locus_tag="LP543_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBL2322812.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHNKATFEKNYKAYVELARDSAKKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1468..1665)
                     /locus_tag="LP543_002695"
     CDS             complement(1468..1665)
                     /locus_tag="LP543_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBL2322813.1"
                     /translation="MVLPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            complement(1681..1923)
                     /locus_tag="LP543_002696"
     CDS             complement(1681..1923)
                     /locus_tag="LP543_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBL2322814.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYELLREKAESAKDRADQAYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            complement(2001..>3602)
                     /locus_tag="LP543_002697"
     CDS             complement(2001..>3602)
                     /locus_tag="LP543_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322815.1"
                     /translation="SIDFGDYDYSGNPNLMANINADSFSQGTGALSVIDDGDEVVVTL
                     DPNHKLELYKMKSQPALVVGETYNMSVEIMLEDDFTGDPSKIALRYFKIPNWVPALTT
                     PNALTTAKGVWQKLTVTAKMTTAIDNAESWYVTLYNQDANNSLSGRLRLRHAKLEEGS
                     TATPYQPNLFDAPYYLSKAALGENIANKSVTFPIKSSDYAVYDYKNIENFVVGEKYVA
                     TIKATKPATQVFEVRNTNSYGFGAMKPVEGLTDIWVLEFTPTRIDAASPSSLQIYQSP
                     KNTKGAVQIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAPEYVENNLAT
                     DIKVAEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKVNELDNKIDERINDT
                     ATTAFYGAQGEVSNKTKVAEYGVGTGYTTTAVGETHFERQSNGYVKCLKAGKYSISAQ
                     IRVQLGGKYASWLYTDLYKDGTSVDNLVSYGVDALQNRFAASGNIVTDLEVNDVLNMR
                     TEIGVADGILQFTSCRTLVLTRLSD"
BASE COUNT         1013 a          745 c          602 g         1242 t
ORIGIN      
        1 tatatgactg gtaatagaag aaaattacta gcatatttta gcgatttacc ctaatttttg
       61 cttctagtta taaaatatta gtactaacta aatttcaaaa cgtagtcaaa aacgtattta
      121 aaattctaat cgggtaagtg ttcgacaaac ggttaatgaa ctgaatttat cttatgtacc
      181 ctgtaggact cgaacctacg accggacggt tatgagccgt ctgctctgac caactgagct
      241 aagagtacag gttgttgcca cataaagcca taaacaatca accagtagaa ggtgtggcaa
      301 caaacctgtt atcgcatatc ttggagtgtg actatttatg agtgatagtg aagatatgtg
      361 acaacatcac tattttatcg aataattttt atagttgtca atatagttat gtaatgctcc
      421 tcaacgagga gctattttta tcgtttagga atatttaaat accaacgttt gtcatggaaa
      481 tcttgcgcac cgcctttagt gtttccctct ggatcattcg ttgcccgcat cattacatag
      541 actttcttac taggaaaatt acgcatattg aaagatacat gataaccaac atttccagaa
      601 gtattataag cttgatttac atctgatcta taaattccat cagctcttac tcgagctaat
      661 tctttcccag tattgtaatc cataatgaag atatactcgt atttatagtt agcaatgtgc
      721 catccagcta catgcaaatt tgcattttcg atttctccaa actgatcaat gtgggcgtaa
      781 ttcgttccat ctgtcagtgt aggattagct gcaccagctc gtgttggatc aatgacaggc
      841 ttgttttcag aagttgttgg attttcatcg gtaaatccat gagctaaatc atatgctaat
      901 ttttctttac ttacgcccat ttcagaaaga taaccgtaag gatctgtatg atcgccccag
      961 atgttttgtg ttacccataa atgtgatttg attcctggtt ggttataagg cgtgtccaat
     1021 gtaagcggaa taccgtattt cttagcggaa tctctcgcca attcaacgta tgctttatag
     1081 ttcttttcaa aagttgcttt attatgtgtg tgttgtaact caatctgcac aggactgttg
     1141 gcattagcat acgaaccagc accgtactgt acataaccag gttgtccaac ttggtaaaca
     1201 attccgccat cacccacaat gtaagcagtg taagcactag tccatgaacg ttgcatatac
     1261 tgcgcttcat tgcgtcctgt tgctgtttca ttagccgttt catgcagtaa aatgtactga
     1321 ttatttgcta cttgtgagct accttcattt gcgcccaaat taaattcatt gttgatagta
     1381 taggcaaacc cattaattgg caataaaaaa agagccgtta ataggctcat cgcagtaata
     1441 gtaattttct ttttcatttg tttcctccta ttttttcaaa ttataagccg acacaccagt
     1501 gataacacct aaaaatgttg ctactgcatt gatagtgagt actgtcatat ctgttccatt
     1561 ccatccatac gctttcccta acgtcgctac taatacagat gcagctggca atactgttaa
     1621 aactgtccat ttaatgactt gataatactt atctggtaaa accatttctt ctcacctcct
     1681 ttacaattta gtcaagaaat agccaatgat tgtaatgcct aaaccgatca tgtaacccca
     1741 cgaccattta ttattggctt tcatctcttt gatgtcttct gcattattaa gcgcaataga
     1801 atatgcctga tccgctctat cttttgcact ttccgctttt tcacgtaata attcgtaatt
     1861 atccagtttc gtttcaatac gcactaagcg ttctaccacg tcttgtattg cttcgtcttt
     1921 caaccaacta gcctcctttc attgcaaaat aaaaaacacc ctcctttgag cgtgttgata
     1981 gcaaaattat aaatattgta ttaatcagat agtctagtca agactaaagt acggcaagat
     2041 gtaaattgta aaattccatc tgcaacgccg atttcagtac gcatatttag tacatcgttt
     2101 acttctaaat cagttacaat gtttccgcta gctgcaaatc tattttgcaa ggcgtcgacc
     2161 ccataagata ctaaattatc aacagaagtt ccatctttat ataaatcagt atataaccat
     2221 gaagcatatt tcccgcccaa ttgcacacgt atttgcgcac taattgaata cttaccagct
     2281 tttaagcatt taacgtatcc gttagattga cgttcgaaat gtgtttctcc tacagctgta
     2341 gtagtatacc ctgttcctac tccatactcc gctactttcg ttttgttaga gacttcacct
     2401 tgtgcgccat aaaatgcggt agtagctgta tcattaatgc gttcatcgat cttattgtct
     2461 aattcattca cttttgtttg agcatcctta gctgtggtgt ctacttcatt aattgaagca
     2521 gtcaactgcg aattaatctc cgatactttc ccatcggtat aattgtttgc tttaccagta
     2581 atttcagcaa ctttgatatc tgtagccaga ttgttttcta catattctgg tgctaaatcc
     2641 cagacatagt cttttggatt atttgagtct cgcatgccga ttcctcggta tttatattgc
     2701 tcaatattcg gggttcgtgt gtcgcctttt tcgatcttga gccagtcgat ttgaacagct
     2761 cctttagtat tctttggtga ctgatatatc tgaagacttg atggactagc agcatcaatt
     2821 cttgttggag taaactctaa tacccatata tcagttaaac cttctactgg tttcatagca
     2881 ccaaaaccat aactatttgt atttcttact tcaaatactt gtgttgcagg ttttgttgct
     2941 tttatagtcg caacatattt ttcccccact acaaaatttt caatgttttt gtagtcgtac
     3001 acggcataat cactagattt aattggaaac gtcacagact tattagcaat attctcaccc
     3061 aaagccgcct tactcaaata atacggtgca tcgaataaat ttggttgata tggtgtagct
     3121 gttgagcctt cttcaagttt agcgtgtctc aaacgtagtc taccagatag gctgttatta
     3181 gcgtcttgat tatatagtgt tacataccaa ctttcagcgt tatcaatcgc agtagtcatt
     3241 ttagcggtaa cagttagttt ttgccataca cccttagcag tagttaatgc attaggcgta
     3301 gttagtgctg ggacccaatt aggtatttta aaatatctta gtgctatctt actagggtct
     3361 ccagtgaaat catcttctaa cataatttct acgctcatgt tatatgtttc gcctactacc
     3421 agagctggtt gacttttcat tttgtataac tctaatttat ggtttgggtc aagcgtgacc
     3481 actacctcgt caccatcatc aataacagat aaagcaccag taccttgcga gaagctgtca
     3541 gcgtttatat tagccattaa attcggattc cccgaataat catagtcccc gaagtcgatg
     3601 ct
//
LOCUS       DAFEPQ010000135         3573 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.142, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000135
VERSION     DAFEPQ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.142"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            115..975
                     /locus_tag="LP543_002698"
                     /pseudo
     CDS             115..975
                     /locus_tag="LP543_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
     gene            987..1814
                     /gene="panB"
                     /locus_tag="LP543_002699"
     CDS             987..1814
                     /gene="panB"
                     /locus_tag="LP543_002699"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBL2322816.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            1829..2677
                     /locus_tag="LP543_002700"
     CDS             1829..2677
                     /locus_tag="LP543_002700"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBL2322817.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            2687..3082
                     /locus_tag="LP543_002701"
     CDS             2687..3082
                     /locus_tag="LP543_002701"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBL2322818.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            3284..>3573
                     /locus_tag="LP543_002702"
     CDS             3284..>3573
                     /locus_tag="LP543_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL2322819.1"
                     /translation="MSRRQRRTYSKEFKQQIVNLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFKKRDNLTPEQAELIALRKKNKQLEMENDILKQAALIFGRKDK"
BASE COUNT         1395 a          394 c          712 g         1072 t
ORIGIN      
        1 atattatgat aaattaacta tggatgatat ttgaaaaaaa catactgtat atttgattta
       61 atttttattt ttaagctcat atcgtaagaa tgggacttgt agtctcactt ctttatggag
      121 gtgataatta tgaacgttgg ttttattgga gccggcaagg taggttgttc attcgggaag
      181 tatttccagg aacataaaat acaagtaaca ggattttata gtaaaagcga agactcgtca
      241 ttagcagctt cgaattttac atctagtaaa caatatttaa acttaagaga attagtagat
      301 gaaaatgata ctattttcat tacgactcct gatggtcaaa ttcaggaggt atggcaagaa
      361 attaaaaatt attaaatcaa aaacaaatta atctgccatt gcagtggtgc aatatcttca
      421 gatatctttt caaatataca ggactatggt gcttatggct attctgtcca tcctatgttt
      481 tcaatatcag ataaatataa ttcatacaaa aaattaaaaa aatcttttat aacaattgaa
      541 ggcgatgaaa aatatgcaaa gtttttatgt gatttcttca aaaagttagg taatagtact
      601 gtaattgtat ctaaggagaa taaatctctt tatcatgccg catcagtagt atctagtaac
      661 cttgtgctag gacttattaa tacaagcgtt acatatctaa ttcaatgtgg atttactgaa
      721 aaaatggcaa ttgaagcttt atatccatta attgaattta atattagaaa tataaaagaa
      781 aaaggaatta tagaaagttt gacagggcca gttgaacgtt gtgatatttc aacaattaaa
      841 ggtcactgtg aagttttatc agatgaagac agagctttat atatgctatt atcaaaaaat
      901 gtattagaga tagctatgtt aaaaaatatt aatagagatt atagtgaatt agaaaagtat
      961 ttaggagaga gttaggagag aaatagatga aaaatacagc agttacattt aaagaatcta
     1021 aattaagaaa tgaaaaatta acaatgctta cagcttatga ctattcaaca gcaaaaataa
     1081 ttgatgaagc tgggataaat ggaatattag taggagattc tcttggtatg gtatgtctag
     1141 gacatgaaga tacactttca gtaacaatgg aagacatgat tcatcatact agagcagtta
     1201 caagaggagc taagaacact ttagttgttg cagatatgcc atttatgtct tatcaaactt
     1261 cagtatatga ttcagtagtt aatgcaggaa gattaataaa agaaggacga gctcaggttg
     1321 ttaaattaga aggagggata gaagtatgcg ataaaataga ggctatagta aaggcttcta
     1381 tacctgttat ggcgcatata ggtttaacac ctcaatctgt taatgcattt ggaggattta
     1441 aggtacaagg aaaggacaag gaagctgcta aagaattaat aagagctgct aaagctgtag
     1501 aaaaagcagg agcattcgct gtagtgttag aatgtgttcc aactaagctt gcagagttaa
     1561 tttctaaaga gatatcaata cctacaattg gtattggagc aggtgcaggg tgtgatggac
     1621 aaattcttgt atatcaagat atgttgggga tgtatagtga ttttacccct aaatttgtga
     1681 agaaatatgc taatttatca gaagagatga ataaagcatt tacaaaatat atagaggaag
     1741 taaaagatgg agtttttcca ggtccagaac atgggtttaa aataagtgat gatgttttag
     1801 aaaaattata ttaaaagggt caagaaatat ggttatattt aaaacagtga atgatgttag
     1861 aagtcaagta aaagagtgga aaaaacaagg cttaaaggta ggattagttc ctactatggg
     1921 atatttacat gaaggtcatg aaagtttaat tagaaaagca tcaaaagaaa atgataaagt
     1981 tgttgtaagt atctttgtaa atccaactca gtttgggaaa aatgaagatt taggtagtta
     2041 tccaagagac ttagaaagag atatagaagt atgcacaaga ggtagagcaa cagcaatatt
     2101 taatcctgaa gttgaagaga tgtattgtga caatgcaagt acttttgtta atataactgg
     2161 attaacagaa ggtttatgtg gagctagtag gccaatacat tttagagggg tttgcactgt
     2221 tgtaagtaaa ttatttaaca taataccagc agatagagct tattttggag aaaaggatgc
     2281 acaacagtta gctgtaatta aaagaatggt tagggacctt aatattgata tagacgttgt
     2341 agggtgtcca attataaggg aagaggatgg actagctaaa agttctagaa acacatactt
     2401 aagtttagag gaaagatctt cagctactat tttaaataaa tctttaactt tagctaagga
     2461 agctttaaat aatggagaga gagacagctt aaaaataatt gagataataa gtaaaaatat
     2521 aaatacttat aatttggcaa agattgatta tgtagaagtt gttgattctt tatcattaca
     2581 aagagtaaat tatattgaaa aatcagtttt agttgcaata gcagtattta taggaaaaac
     2641 aagattaata gataatttta catttgaatt acaatagggg gctataatga aagttacaat
     2701 gttaaaagga aaaatacata gagctacagt tgaacaagct gagttagatt atgtaggaag
     2761 tattactgtt gatatgaatt tattagaagc agcaggaata tatgaatatg aaaaagtaca
     2821 aatagttgat gttaataatg gaaatagatt tgaaacttat acaatagctg gagagagagg
     2881 aacagggatg atttgcttga atggagctgc agctagatgt gtatcaactg gagataaaat
     2941 aattttaatg gcatattgtg aattaaatga aaatgaggtt aaggatcata gacctaaggt
     3001 tgtttttgtc gatgataata ataaagttga aagagtaaca tcatatgaaa aacatggaag
     3061 gttatcagat ataattcttt aggttttgta atgttattta tatatagcta gactattatt
     3121 gaaatattta ttgattacta attggataga tgtcaataaa gtagacacaa aaagagaaga
     3181 tacagactgt agacaaagtc cattttatgt gaatcggcta cactagacta gacagaaaaa
     3241 ataaggtgtg tagaataata aaaaacacac tggaggattt ttcatgtcaa gacgtcaacg
     3301 aagaacctat tcaaaagaat tcaaacaaca aatcgtcaat ctctatctcg ctggtaagcc
     3361 tcgcgcagaa attattcgag agtatgagct tacgccttct tctttcgata aatggatgaa
     3421 gcaagcacaa tcaacgggct cattcaaaaa aagagacaac ttaacaccag aacaagcaga
     3481 attgatcgca ctaagaaaga aaaataagca actcgaaatg gagaatgata ttttaaagca
     3541 agcggcgctg atattcggac gaaaagacaa gta
//
LOCUS       DAFEPQ010000136         3399 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.143, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000136
VERSION     DAFEPQ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.143"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..277
                     /locus_tag="LP543_002703"
     CDS             <1..277
                     /locus_tag="LP543_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HBL2322820.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="LP543_002704"
     CDS             420..641
                     /locus_tag="LP543_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2322821.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="LP543_002705"
     CDS             657..1499
                     /locus_tag="LP543_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322822.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="LP543_002706"
     CDS             1562..3364
                     /locus_tag="LP543_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HBL2322823.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYCWKYKD"
BASE COUNT         1229 a          469 c          703 g          998 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactgctgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaata
//
LOCUS       DAFEPQ010000137         3390 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.144, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000137
VERSION     DAFEPQ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.144"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(65..2557)
                     /locus_tag="LP543_002707"
     CDS             complement(65..2557)
                     /locus_tag="LP543_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBL2322824.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2564..3031)
                     /locus_tag="LP543_002708"
     CDS             complement(2564..3031)
                     /locus_tag="LP543_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBL2322825.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(3167..3240)
                     /locus_tag="LP543_002709"
     tRNA            complement(3167..3240)
                     /locus_tag="LP543_002709"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3204..3206),aa:Arg,seq:acg)
     gene            complement(3298..3371)
                     /locus_tag="LP543_002710"
     tRNA            complement(3298..3371)
                     /locus_tag="LP543_002710"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3335..3337),aa:Pro,seq:tgg)
BASE COUNT          925 a          750 c          557 g         1158 t
ORIGIN      
        1 atcttgtcac aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa
       61 gtaactatag ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac
      121 cttttgaagc acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct
      181 cgctcaatcg atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt
      241 caggatcaaa tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa
      301 tatcttgttc tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga
      361 tttcatcttt actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag
      421 gtcggaaagc tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag
      481 taacatcttg gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat
      541 ttgaagtcat aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta
      601 aatgaccatc atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca
      661 cttcatctaa gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac
      721 cttcatcata ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt
      781 ccataaattc tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg
      841 aaagtgcttt ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac
      901 caattggacg atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg
      961 ctttgacagc ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca
     1021 agcgttcgct ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa
     1081 ctgtagcaac atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa
     1141 ttgcagaaac tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag
     1201 cattttcaaa gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt
     1261 ctagtttgat cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg
     1321 ctgattcatc gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa
     1381 cagaaagatt gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt
     1441 catatcttgt acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta
     1501 cttgaatgcg agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt
     1561 catctagggt cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga
     1621 tattcgaagc atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat
     1681 caatgaataa gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat
     1741 cttcaaattc accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca
     1801 ggcgcttttc ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt
     1861 cagcaatggc tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc
     1921 gtcggctaag aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca
     1981 gcttgttttc ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc
     2041 cttgcggtgt ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc
     2101 cagcagaatc accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc
     2161 tgatacctaa gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca
     2221 tcaaaatatg ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta
     2281 aagcaaaaac ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac
     2341 ggataccacc ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat
     2401 ccatttcacg taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa
     2461 catgttctga actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta
     2521 atactgcttg ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat
     2581 aacttaaacg ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat
     2641 tgaaattcaa tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa
     2701 ttatttggtc ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat
     2761 catcaaataa ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg
     2821 caattcgtat ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg
     2881 taaaacgggt attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca
     2941 tttcaatcct tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt
     3001 caattaaatc cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac
     3061 cttaattata acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc
     3121 tttagaatca acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga
     3181 gtcgaacccc taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc
     3241 atattcaatt taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata aaaaaaatcg
     3301 ggaagacagg attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga
     3361 gctacttccc gtttaaaaaa taaaaaagac
//
LOCUS       DAFEPQ010000138         3390 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.145, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000138
VERSION     DAFEPQ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.145"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            647..964
                     /locus_tag="LP543_002711"
     CDS             647..964
                     /locus_tag="LP543_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBL2322826.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1055..1420)
                     /locus_tag="LP543_002712"
     CDS             complement(1055..1420)
                     /locus_tag="LP543_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322827.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1628..2110
                     /locus_tag="LP543_002713"
     CDS             1628..2110
                     /locus_tag="LP543_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322828.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2307..3197
                     /locus_tag="LP543_002714"
     CDS             2307..3197
                     /locus_tag="LP543_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HBL2322829.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1172 a          519 c          619 g         1080 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct
      601 aaacataatt ttttcggtat ttaatattta attaggaggt aatattttgc ttttttatag
      661 taattttatc ttaatagtag cgatattatt acttctgaat atatggattt ttgataggag
      721 tagaaattca agtataggtt ttagaactaa acgaagtttg tcttctaaaa aaaattgggt
      781 atactcacaa actatttttt atggaggaat tgttttaatt agtttacttt cttcaacact
      841 ttattctttg aatattatag atgtttccac ctcaaattca atatctatta ttggaatcat
      901 tattgctgca attattacac aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa
      961 atagaaaaac tagtgatagt tttggtgata taagaaaaac atatatgtat aaaaagaaga
     1021 aagtagcttc tctttgtaca tatatggttt gtttttatgg atgccgagct tttttataag
     1081 ataaggctac cgcccataac gacccaagga tttgtggaac taagtaaata cctgcaagaa
     1141 gcaagatcag ctgggtcggt tttaaaggat gatagaagta acgccattca gtaaaattgg
     1201 cgcaaaataa ataggaagtg acagtagata taatattggc aatgaataaa gatataatac
     1261 gcctgttcct ttgaaaataa tatccagcaa aaagagataa aaatatcaat gtgatgaacc
     1321 cgcttacgga atgataacga aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag
     1381 ataaaagcaa cggaaggttt ttccagcaag tttttatcat ctttcatcac cacctaacaa
     1441 gtgttattat tttgttagtt ttattgtaaa ttgaatcctg caataatcaa ttactttttc
     1501 attatttcta gattaaatga ataaactctc atgccattat ctctaaaaaa aaagtttttc
     1561 cccctaattt ttttagatag aaaattgtat agtatgatga gagaaaaagt agtttggggg
     1621 aacgttaatg agaagaaaag cagtaatcga tcggctttat ttattttttt ggttttttcc
     1681 tatcgttttg ctacctttta catgggtaaa tgctgttgta caaggtgcgg agatattagg
     1741 cctgatccaa ggaagcggat ttgtggtttc taggaatttt ccagctttga ttgttttcta
     1801 tttagtgata agtgtaggtc tcttattacc aaaatctctg atcaatcact acaattggca
     1861 gctgctttac ttttcttgct tattagtatt tacatctatg ttttctttgc tgcctcgctt
     1921 gattatcggc cctaatggtt tgagcgagag tggattgaag gatttagctt attcctccat
     1981 gtatttcttg acgatcaaac catggttcta tttagtaatt gcctcattta ttcttgcaac
     2041 atttttttac ttctggacag aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa
     2101 taactattga tggtggatca tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc
     2161 tcatatgctt tttttattat tatgtaaaga atgcgaaaaa cgtttacatg atttttacat
     2221 aacttaacta tatttttaca gcaaccatac gagggattta cagtcgactg ataaacttta
     2281 gttgtaaata agaaaggagc aataaaatga aaaaagtatt gtttactcta ggtatgatgg
     2341 gaattctttt agccggatgc ggctcaggat caggaaatac agatggctct gccacacaaa
     2401 ataattcaag tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg acagcattac
     2461 agccgttagt cgatgcagca caagaaagct ttgtacaaga aaatccaaat tatcaaatct
     2521 ctgtacaagg aggaggaagc ggaactggtt tgagccaagt cgaagctgga gcagttacta
     2581 tcggaaactc tgatgtattt gcagaagaaa gagacggagt tgatgcttca aaattagtgg
     2641 atcataaagt agcagtggtc gggatggcac caatcgtcaa taaagataca gatgtcaaag
     2701 atatcaccaa acaggaattg attgatatct ttacaggaaa aatcactaac tggaaggaag
     2761 ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg
     2821 cgacattcga aaaatgggga ctggatggtg ctacccctgt gcagtcccaa gaacaagatt
     2881 catcaggaac agttcggcag ctggtaagtc aaacaccagg agcaatcagc tatttagctt
     2941 tctcatatct cgatgattcc acacaagcac taagtatcga cggtgtagaa ccaaaagaag
     3001 agaatgtagc agataacagc tggggaatct ggtcatatga acatatgtac acgaacggaa
     3061 aaccttcccc tgaagtccaa aaattcttag actacatgat gacagaagaa atccaagaag
     3121 gacctgtcaa agaattgggc tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg
     3181 aaggaaatat caaataacta gcagataaac atgccacggt agtcgttact attaaggaaa
     3241 aatcaactat gtttccccac atatacattt ttcctttaat tgtgaaaaat agttcctcat
     3301 attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt ttattcgaaa
     3361 aaaagagcta cgacatagaa ctgtcgtaac
//
LOCUS       DAFEPQ010000139         3373 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.146, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000139
VERSION     DAFEPQ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.146"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="LP543_002715"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="LP543_002715"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBL2322830.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="LP543_002716"
     CDS             complement(2260..2511)
                     /locus_tag="LP543_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBL2322831.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="LP543_002717"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="LP543_002717"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBL2322832.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          920 a          739 c          626 g         1088 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttt
//
LOCUS       DAFEPQ010000140         3306 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.147, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000140
VERSION     DAFEPQ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.147"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(20..1819)
                     /locus_tag="LP543_002718"
     CDS             complement(20..1819)
                     /locus_tag="LP543_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBL2322833.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDSKVRETLFAAWEEA
                     WEEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            1942..2448
                     /locus_tag="LP543_002719"
     CDS             1942..2448
                     /locus_tag="LP543_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL2322834.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            2527..3249
                     /locus_tag="LP543_002720"
     CDS             2527..3249
                     /locus_tag="LP543_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2322835.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
BASE COUNT          991 a          595 c          693 g         1027 t
ORIGIN      
        1 tattgtgata aaatagaaat cagataaatt cttctgtcaa ttccatgaac gtttggatat
       61 cttcagtcac catgtcgata cctgcttgcc aaaaatctgg ttgggtaaga tcgacattca
      121 aatgtttctt cgcaagatct tccgtcgtca ttgaggcagt atcgcgtagt aaatggatat
      181 attcttcttc aaaatttgct cccttttggt tggcgtaagc atagatgccc aaactaaaga
      241 ggtagccaaa tgtgtatggg aagttataaa acggtacgtc atcaataaag aaatgcaatt
      301 ttgcagccca gaaatatgga tggtaagtag ctaaagctcc ttgatagcct tctttttgcg
      361 cctcaaccat catctctgtg atcttatctt ctgagactaa tccttcttgc cgtgcttcat
      421 agaatcgatt ttcaaaaatg aatcgggaat ggatattcat gaacatagct aaagcatttt
      481 gcagttttgt atctagcaaa ttgatttttt cttcttttgt ttttgcgact tttaatgtag
      541 catcggctac gatcagttct gcgaatgtac ttgctgtttc tgcgacattc atggcatatt
      601 cgcgatttaa agaaggtaaa tcccacatcg tgctgctatg gaaagcatgt cctagttcat
      661 gagcaagtgt cgcaacctca ttaacagaat tactgtaggt cataaagatc cttgattctt
      721 ttgtttctgg taactcagta caataaccac caggacgttt cccaggacga tcttctgctt
      781 cgatccagct cttttcaaaa gcagattggg caaatttagc catttttgga ctgaatttgt
      841 taaagttttc gataataaat gctgctgctt cgtcaaaact aaaggttttt tctttcaaat
      901 cgcctaaaat aataggtgca tcttgatctt gccattccat tttttcttta ccgaaaagat
      961 tagcttttct agtcaagtaa ttaacaaggg gttgtttgtt tttctggatt gtatcccaca
     1021 tgacagacaa tgtttctttc ttcaaccgat tgtactctaa tggtttttgt agaaaatctg
     1081 tgacaccatg aagttcatag tcagaaagtc taaaaccatc taaatgattc aaagtatccg
     1141 tgaagagggg tgccttttct tcccaggctt cttcccatgc tgcgaaaagt gtttctctga
     1201 cttttgaatc tggatcgccc atcatcttat tgaaggcttg tccagcagat aattcgacta
     1261 cttgtccatc ttgttcgaag ggaatcgaaa tgctggctac aatcgtatca taatgactgc
     1321 tccaagcatt tagaccatct aaagaaagcg tatttataat gttttcttca gcttctgata
     1381 ataattgtga gccatcacga cgaatctcgt ttaaacgaaa agcaattgtt tcaaacgaag
     1441 attgagaaag catctgattc caatcattgt cagatatttc tgcaaatttc tttgacaata
     1501 cagtttcggc tgattgcaga cgaggcagca ttgcgtaaag cttgcctgat aagacctttg
     1561 catctgaatc attgacattg gcagaaagca atgcagtaat atagctgctg cattgggtaa
     1621 aaccattcgt aatcgtttct tgcaattgaa gaatagcgtt taactgctct gcattgtcag
     1681 aactaggtga ccattttgtt acgcggtgat aatactcgtt catttggtct tccaattgct
     1741 tcatccgttg gttcaaagca tcagaatgac tgcctccgga aaaaatagaa tctagattcc
     1801 aatttaatga ataactcatg aaaaaactcc cttcatattg taatacaaaa ttaagtatag
     1861 cactttttta tggaagaagt ctctatgaaa atgattgttg tttcttcaaa aaaagtctaa
     1921 gataaaatac agaggtgact tatgaattct tcaaaaaaac aaatgaaaaa cggaaatatt
     1981 tatttagtaa tcgcactgat agtcgttata atcttatttt acagttcttc tcaaacttac
     2041 ggacagcaat cacaagtttc gcgattagaa agctggctgc ctaatcagcc atttaaagac
     2101 acattagcgg gtatacgttt taattatggt ggaagtatgg tgagtatcga ccatctgggc
     2161 tacgccaaat ttttagagtt ctttttaagg aagggcgcac atttcggtac ctattttatt
     2221 ttgggaggca gcttgttttt aggtgttttc cccaagctaa aaatttggtg gctgacagcg
     2281 atccttgctt ggctttcagc aacagggtac gcaggtttgg acgaattcca tcagatgatg
     2341 accggtggac gaacacctat gttccaagat gtcatgttgg attcgatcgg tgctctgact
     2401 gctgtgatat tatgtgtgct ttttgcggca ttcaagaagc gtttatagat tggctgtggc
     2461 tttgtttttt tgtgaaatag tactagaata gaagtgtatt ctcaaaagaa caagggagga
     2521 aaaatcatgt caggacatag taaatggcat aatatccaag gaagaaaaaa tgcccaagat
     2581 gcaaaacgag ggaaagtctt tcagaaactt tctcgtgaaa tttatatggc agctaaagca
     2641 ggcggtcctg atccatctat gaatccagct ttgcggcttg ttgtggacaa agcaaaagct
     2701 gcaaatatgc ctaacgacaa tgtggaacgg gcgattaaaa aggctacgag tgccgtagat
     2761 gaaacgaatt acgatgaaat cacgtatgaa ggatatggtc cgggtggagt aggaatctta
     2821 gtccatgcat tgacagataa ccgtaatcgg acagcgacaa atgtccgagt agcttttact
     2881 cgaaacggtg gatcactggg agaaacgggt tcagtcagtt acttgtttga ccgcaaagga
     2941 tatatcgcta ttgaacgcga tgggttgact gttgatgaag agacgatgtt tgaggatgta
     3001 ttagaggcgg gggcagaaga tctgcaaact tctcctgaag tattcgagat ttatactgca
     3061 ccagaagatt ttgctgctgt acgagatgag ttggaaaaag agttcacttt ggcgcaagca
     3121 gaattgacga tgattccaca aacgacgatt gatttaaatg aggaacaaaa agagcaattg
     3181 gaacgtctag tagataagct agaagacgat gatgatgttt cagaagtctt tactgctgct
     3241 gaaatgtaag tagcacattg ttagataata aaaagaaagc acctgtacca atcgtacggg
     3301 tgcttt
//
LOCUS       DAFEPQ010000141         3293 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.148, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000141
VERSION     DAFEPQ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.148"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            38..589
                     /locus_tag="LP543_002721"
     CDS             38..589
                     /locus_tag="LP543_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HBL2322836.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            1265..1519
                     /locus_tag="LP543_002722"
                     /pseudo
     CDS             1265..1519
                     /locus_tag="LP543_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1589..2101
                     /locus_tag="LP543_002723"
     CDS             1589..2101
                     /locus_tag="LP543_002723"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL2322837.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            2098..3135
                     /locus_tag="LP543_002724"
     CDS             2098..3135
                     /locus_tag="LP543_002724"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL2322838.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1213 a          555 c          654 g          871 t
ORIGIN      
        1 gagcgagcaa gacaggaacg agagaacaaa catactcatg aaaagactag aggcttcgac
       61 ctcgaacttt aataataagt tagatgtttt tgaagaaaag actaagcatg caagattaaa
      121 ctttgaaaca actgcaaagc actatattaa acgacttgat gaagataatt tgaaattaga
      181 ttttcaacaa gctattcaag atgaattaag cgatactaaa gacgaaatac gtgaagtgac
      241 gaaacaagcg agagaagaaa caaaagagta taaagaaatc ttagaaagta aaataaaaga
      301 ccataacaaa gtggttgata aatcgaatac agcgctaaaa gtgatgacaa aaggcgttac
      361 aaatatattt tttgtgctaa ttatttttgt acttgtaatg cttgtgacag gtccgatagg
      421 tcatttcttt ggtattgaac atttatatag ctttattaat ggttttattg atgaccacga
      481 aagtgcatgg cgatatttga tgttgatact ttatgcagta ccctatgtat tcttcgctat
      541 cattctgtgg ctcatagcat tgttttttag agtatttaat gacctttagg tagatttata
      601 tataaaaagg gctaaatacg caaaataccc ccttaaaatg caaaataaat gcaaataagg
      661 gggtaactcc ttatgaaaaa acaactaata gtattataac acgtttttat gccgagaaaa
      721 cttattggtt ggaatgggct atgtgttagc taacttgtta gcgagttggt tggacttgaa
      781 ttgggattaa tcccaagaaa gtaccaactc aacaacacat aaagccctgt aggttccgac
      841 caataaggaa attggaataa agcaacgaaa ggagttgagt aaaatgagtg agaaaacgaa
      901 gaaaatcatt cgagtatcat tagctttttc acaagtagta gtgcaatcat tgatagttta
      961 ttatacttac cagcaatata aattatctaa agagaattaa gttttgatta tgtaataagt
     1021 tttggtacta aaaaagactt gatctaatta gaccaagtct tttgttagtg ttatattaat
     1081 aacaaaataa aaataagaag tcgctcactc cccgaccaaa gtttgtgagc aaaataaaca
     1141 gtcgatgttt gtttcttaat tgtatatctt gatattaaac gatttaagat tattcttcaa
     1201 gatatatatt cgcaaatgta attggaaaag cgacttctta aacaaaatta aggagtcgct
     1261 ttttatggca aaaataacga aaaatgagca agaaaatttg gtggaaaatt acacaaaaaa
     1321 accggcgtac tctaatagcc ggttagacgc acatttcgtg tgcacttcta atccgaaatt
     1381 gagctttgac gctatgacga tagtcggaaa ccttaataaa aacagcgcta aaaagctatc
     1441 cgattttatg agtttagatc cacaaattag actttgggat atacttcaaa caaaatttaa
     1501 agctaaagca ctacaagacc ccctatgtta ggctagttgt cgtataggat aataagttat
     1561 aataaagact atacggagga tgattaccat gacaaataaa aacaatgaac gttactctac
     1621 cgaactgaaa gcgacagcca tcaagaaaat gatgccaccc gaaaataggg ggattcgcca
     1681 attaagtcaa actttgggca ttccagaagc gactctttgt tcttggcgta aaaaagctag
     1741 aattcaaggg aatactacac ccggaacgaa aaaaaatgtc gaaaaatggt caagtgaaga
     1801 taaattttta attgtgatgg aatcattctc aatgaaccaa gtagaacttg cggaatattg
     1861 ccgaaagaag ggcttattta aagaacaaat tgaggcttgg aagaaaacgt gcttatctgc
     1921 gaatgaacag gaagaaaatc gaacgagaga acttgcaact gaactaaaag aagagaaaaa
     1981 acaagcgcgt caattagaga aagaattacg tcataaggaa aaagcattag cggaagccgc
     2041 tgctctctta ttactaagaa aaaaagcaca cgcgatttgg ggggaccaag aggaagaatg
     2101 atttcatctt cagatcgcaa acaagctgtt gaacttattc aagaagccaa tcgcaatggg
     2161 gcccgtctca cctatgcctg taatgagctg aatattagcg ttcgaaccta cgaacgatgg
     2221 acgcgtgaag gtacgatagc gcatgatcaa cgccctttgg cgaaacgtcc tgtacccaaa
     2281 aataaactga cggatcaaga acgcgaaaaa ataatagaaa ccgtttcaaa aaaagaattt
     2341 atgaatttgc cacctagcca aattgtacca aaacttgcgg attgctcgat ttatattgca
     2401 tcagagtcta gcttctatcg tgtattgcga gaaaaaaata tgcaacatca ccgtggccga
     2461 agtcaagttt cacaaaaacg cataccgcca agtcaccttg cgacgaagcc aaatgaagtt
     2521 tggacttggg atattacttg gctaaaaggt ccaataaaag gactctttta ccggttatat
     2581 ctcatcattg atttatttag ccgtaaaatg gtaggctggg agatttggga atcagaagaa
     2641 gctcgtcatg ccgaaaaact tattaaacag acggtgctta aggaacgtat ccaaggacag
     2701 ccgttggttt tacactccga taatggcagc cctatgaaag ccgcgacttt tcaagtctta
     2761 ctcgaaaaat taggaattca aagttcttat tcaagaccac gagtgagtaa tgacaatcct
     2821 tattcggaag cggctttccg aacgttaaaa tatcgaccag aatatcccaa taacggcttt
     2881 aaaacaatag atgatgctcg aaaatggacg aaagagtttg tcgattggta taacgattct
     2941 catttgcata gtgggattca atttgtgtct cctgcagcgt gtcatgaagg acgacatctc
     3001 gctattttag agaaacgaaa gcaggtttac gaggacgcta aaaagcgcca tcctgaacgc
     3061 tggtcaaaag gtgttcgaaa ttggactcca catcaatcgg tggcgttgaa tcctttaaaa
     3121 gaaacagaaa gttaacagga gcataatctt tcaagattat caaataaccc tcgtcaccca
     3181 ataggggaaa aagttattct tcccctattg gataacgagg ccaaccgacc atagggaggt
     3241 tctgcttttt gataggtgga aaataaaaat acgacaacta tattgacaaa cac
//
LOCUS       DAFEPQ010000142         3232 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.149, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000142
VERSION     DAFEPQ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.149"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            184..1138
                     /locus_tag="LP543_002725"
                     /pseudo
     CDS             184..1138
                     /locus_tag="LP543_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
     gene            1155..2179
                     /locus_tag="LP543_002726"
                     /pseudo
     CDS             1155..2179
                     /locus_tag="LP543_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            2185..2664
                     /locus_tag="LP543_002727"
     CDS             2185..2664
                     /locus_tag="LP543_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBL2322839.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            2680..>3232
                     /locus_tag="LP543_002728"
     CDS             2680..>3232
                     /locus_tag="LP543_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBL2322840.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVG"
BASE COUNT          980 a          539 c          751 g          962 t
ORIGIN      
        1 acttggaaaa cgttgtttct ccaagttctt tttttaattt ccaagtaact gaaagttact
       61 tttgttattt aacatttagg tcaattttag acagtttttg ttcaaaaagc gatatttata
      121 gtggacggta ttactgatat attaaggata taaaatgaaa gcgtttaaaa aggtggcgta
      181 aagatgatta ttaaagaaga gatttttgta cctaaagaat tagtgaaaga aaaaattgac
      241 ctgctcgttg aaaacctgac aacgatcaaa gacgataatg gtgaattttt gttgggcttt
      301 gatggactga aagtagatga caagagctgg acagtgtgga attggccaca aggtgtcggt
      361 ctatatggta tttataaaaa ttaccagatg acaaaaaatc ctcgcgctta tcaagtggtc
      421 aatgaatggt ttgaggatcg tatggaagaa ggcgctccgc ccaaaaacgt caatacaatg
      481 gcaccgctat tgactatggc ttacttgtat gaagacacga aagattcacg ctatttgcct
      541 tatttagaac aatgggcaga atgggtcatg aatgacatgc ctcggacaaa tgaagacggg
      601 ttacagcatg ccacatatgg accggaaaat aagaatcagt tgtgggacga tacattgatg
      661 atgacagtgc ttcctttggc aaaaatcggg aaactgctgg ttgacgattg ctattccaga
      721 gatcattgaa atcttagaat tatcgaaaga agatgcattg cgcaaattat tgatcgaaac
      781 acttgaagca caagtgcgtg cattaaaaac ttatcagtct gaaagcggtt tatggcatac
      841 gttgttggat gatccttctt cttacgtaga atcttctgca actgcaggat ttgcttacgg
      901 tattttgaaa gcggtccata aacgttattt accacaagaa tataaagaag tcgcttatcg
      961 tgctatccaa ggattgcttg aacaaatcga tgaaaaagga gaagttcaaa acgtttcaat
     1021 cgggacagga atgggggaca gtttagattt ttatcggaat atcggaatca cagcaatgcc
     1081 ttatggacag tcgttaacgg tgctttgttt gacagaattg ctcgtctctt attgttaaaa
     1141 gaaaggaaac ccatatgaaa aaaacatcac tgctgtttgc agcaagttcg cttagcctag
     1201 gtctgctagg tctaagcggc tgcggaaata attctagtgt ggcaactgtc gcagatgcag
     1261 acgtaaatga gatagttacc ttgcgctttg cttatgcaag caacagccag taattgatgc
     1321 aatgaatgaa tttgggcgtt tagtaaaaga aaaaacagat ggttccgtac agatccagta
     1381 tttcccagat ggacaactgg gtggagaacg tgaactgatc gaattgacac agtcaggagc
     1441 agtagatttc acaaaagtaa gtgcctcagc gttggaaagt ttctctaaag attattcgat
     1501 tttttccgtt ccttatctat tcaatgatga agaacatttc tttaaggtga tggataatga
     1561 agaaataatg gagccaatct accaatcgac caaaaattta ggatttaccg gattgaccta
     1621 ttatgattct ggtcagctca gcttttatat ggtagacggt ccgatccata cgccggaaga
     1681 tttgaaggga aagaagatcc gtgtgatgca aagcgaaacc gctatcaaaa tggtagagct
     1741 tttaggcggt tcgcccgtac ctatgggaag tgatgaagtg tacacgtctc ttcaatccaa
     1801 tttgatcaac ggatcagaaa acaatgaatt tgtcttgtat acagctggac atggcggggt
     1861 agctaaatac tattcctatg atgagcatac acgtgtgcca gatatcatca tcatgaatga
     1921 tgccatcaaa gaacagctaa ctgatgaaca ggaaaaagcc attgaagaag cagcgaaaga
     1981 atcaacggtt tttgaaattg aagcttttgc aaaagcaatt gaagaagaaa aagctttagc
     2041 acaagaagaa catggagtcc aattcaatga tgtagataat acaccgtttc gagaggcagt
     2101 tgccccactt catgatatat tcaaaaacga tcctacctac aaagaccttt atcaaaagat
     2161 ccaagagcaa ggagcgtagc tgtaatgaaa atgattttaa acaaggtatt agagatcctg
     2221 ggagctgttt tgcttgttgg gatggtcgct gttgttttat ggcaagtatt ttccagaacg
     2281 gttttaggaa atccaaatac ggtcacggaa gagcttgttc gattcggtct tgtctggttt
     2341 gccatgttgt cttccgcgta tgttgtggga caaaaagcac gtttagccgt aacggtttta
     2401 agtgaaaaat taactggcaa aaaaagtaaa attttagaag ttgttgttca aagtttattt
     2461 ctggtatttg ctgcgatgat catggtgtat ggcggatgga atgctgtcac attgacaatg
     2521 gggcagatct cgccttcttt aagtattcct atgggatatg tttatctgtc agtacctgta
     2581 tcgggggtac tcatcattat ctatagcctg atcaatctaa tcgatacatt aagcaacaag
     2641 ccagcaattc aagaaaaaat ataaaaatgg aggaataaaa tggcaataga agccggatta
     2701 gtgttatttt ttgtatttgt tgtgttgctt gtattaggga tgccgattgc tataagtatt
     2761 gcattctcat cgatgatgac tttattgtta gttataccgt ttgatgtttc tgcttttagt
     2821 tcagctcaaa aaatggtggg aagtatcaat agtttttcgc tgatcgctat tccgtttttc
     2881 gttctctcgg gcattatcat gaataatgga gggatagcta agaaactagt ggattttgcg
     2941 atgttgttcg tcgggcgcgt gccgggggct ttggctcata cgaatgtgtt agggaatgct
     3001 ttatttggtt ccatctctag ctcggccatt gccgcttcta ccgcgattgg tggagtgttg
     3061 atccctcagc aagtggaaga aggatatgac cggaagtttg cgacagcggt caatatcgcg
     3121 tcagctccga caggtatggt cattcctcca agtactgctt ttatcatgta ttcacttgtt
     3181 gctggtggtg cttcgatctc tgctttgttt atgggaggtt atctcgtagg cg
//
LOCUS       DAFEPQ010000143         3218 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.150, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000143
VERSION     DAFEPQ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.150"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            50..1438
                     /locus_tag="LP543_002729"
     CDS             50..1438
                     /locus_tag="LP543_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HBL2322841.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1776..>3218
                     /locus_tag="LP543_002730"
     CDS             1776..>3218
                     /locus_tag="LP543_002730"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBL2322842.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT         1183 a          505 c          582 g          948 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttatat
     1501 cggaagtaat ctcttgattt ctatactata agataagaaa accactattt aacaaggtta
     1561 ttcgaacctt attttaagtt acttattatt taatgtatcg aaaaaggtga aataggtttg
     1621 ttataataag aattaagtaa gacatgaatt gtctaagcaa atgtagctta tccatttcat
     1681 taaaatacat atggtgaaaa aacaagttca aatagtaact aattttttaa aaaactgatt
     1741 tataaaaaat acaacacgaa tgaggaaaat aaccaatgac agaacaaatc gaaaaaaatt
     1801 caaaaagctt atatcaggca ctgtggaatt ccgcagatat cctgcgttca aaaatggacg
     1861 ctaatgaata caagagctat ttactaggct tggtctttta taaatactta tccgacaaca
     1921 tgctgcggta tgtctccgtt ctattggagg aagaaacgga cgacctaaat aaagcacaag
     1981 acctttacgt tgaagcattt aaagatgcag atataaagga tgatctcttg tatgaactaa
     2041 aagatgagtt ttcatatacc atcgcacccg cattgacctt taccgcgcaa gtggcagcca
     2101 ttcatgatgg atcttttcaa ttagaagacc tggtacaagg tttccgtgac attgaacaat
     2161 ccagcgaaaa ctttgagaac ttatttgaag acattgatct gtattctaaa aaactggggt
     2221 caaccccaca aaaacaaaat aaaaccatcg cagatgttat gaaagaatta gaagggttaa
     2281 acatggcggg gcatgctggc gatgtcttag gcgatgccta tgagtactta attggccaat
     2341 tcgcatctga atccggtaaa aaagccggtg aattctatac cccacaaccg gtcgcaaaat
     2401 tgatgacaca aattgtttta caaggaaaag aagataaaaa aggcttttct gtttacgacg
     2461 cgaccatggg ttcgggctcg ttacttttga acgctaaaaa atattcccat caaccgggaa
     2521 ccatttctta tttcggacaa gagttgaata cgtctaccta taatttggcg cgtatgaaca
     2581 tgattttgca tggggttccc attgccaacc aacacttgca taatgcggat accttggatc
     2641 aagactggcc aacagaagag ccaacgaact tcgatggggt gctcatgaac ccaccgtatt
     2701 ctgcaaaatg gtcggctgat aaaggcttct tagatgaccc acgcttctct gcctatggcg
     2761 tattagcccc taaatcaaaa gcggactttg ctttcttatt acatggctac tatcatttga
     2821 aagatacggg cgtcatggcc attgttttgc cacatggggt gctctttaga gggggcgcag
     2881 aagggaagat acgcaaagcc ttgttagaaa atggtgccat tgacaccgtc attggcttac
     2941 ctgctaacat cttctttaat acgtccattc caacgaccgt aattatcttg aagaaagacc
     3001 gtgcgaaaaa agatgtgctc tttattgatg cgtcacaaga ttttgaaaaa ataaaaacgc
     3061 aaaatacgct acgagattac catattgacg ctattttaga ggcctataaa acccgtacaa
     3121 ttaaagacaa gtatgcttat gtcgcagaat ttgatgaaat tgtggagaat gattacaact
     3181 taaacattcc ccgttacgtg gatacctttg aagaagaa
//
LOCUS       DAFEPQ010000144         3199 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.151, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000144
VERSION     DAFEPQ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.151"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(<1..114)
                     /locus_tag="LP543_002731"
     CDS             complement(<1..114)
                     /locus_tag="LP543_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBL2322843.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDR"
     gene            complement(187..588)
                     /locus_tag="LP543_002732"
                     /pseudo
     CDS             complement(187..588)
                     /locus_tag="LP543_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1241..1336
                     /locus_tag="LP543_002733"
     CDS             1241..1336
                     /locus_tag="LP543_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBL2322844.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            2037..2321
                     /locus_tag="LP543_002734"
     CDS             2037..2321
                     /locus_tag="LP543_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322845.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            2324..2740
                     /locus_tag="LP543_002735"
     CDS             2324..2740
                     /locus_tag="LP543_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322846.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2877..>3199)
                     /locus_tag="LP543_002736"
     CDS             complement(2877..>3199)
                     /locus_tag="LP543_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBL2322847.1"
                     /translation="SAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIPEYY
                     QMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKISLRN
                     WLEE"
BASE COUNT         1068 a          602 c          504 g         1025 t
ORIGIN      
        1 acgatcatat aacaattcct gaacttcacg atagcttaga ttgtaacgca ggtagtaacc
       61 aacagcgaca ataatgacgt cttttttgaa ttgtttgcct ttaaaatgat tcattactct
      121 gtcctctctg tcttttttct caattttaca ctaaaataga ttttttggaa aactttgcaa
      181 cagaaccgat aattcaaatc tactaaattc agctgttaaa ccattaataa tatattctag
      241 gtccggatta acactgactt caacaaactt ttttgtttta ttgatttcaa aaccagtaaa
      301 caaaacaaag tagcgaatag aatcctcatc ttcagtacgg taagtaagat taagcatatc
      361 tttatagagc ttttctaaat caccgacaaa ggcttttgta gcagtcatct tataatcact
      421 taactctttt aattcctcga aagaaaatct aacttttttc aaaccacgat ctttcatacg
      481 agcgcataat gcaaaaaata aatccatctg cttagcattg aaatttttca gcggtactaa
      541 attcaattca ttttgatatt tgactgttaa ttccgttttt tcatccattc ctttcttccc
      601 cccttacaaa taaataataa cctttaacaa ccaactcgta aataggttgg ttgttattat
      661 acaaaaggat attagtactc agataaaaag ttgttagtac tcagataaaa agttgttagt
      721 actcagataa aaagttgtta gtactcagat aaaaagttgt tagtaccctt ctgtatccgt
      781 tgggagagta aggctcaact gccccctaaa aagggttcta aaaaatcttt aaaaaataaa
      841 taaaataaac gcgcgcgcgc gagcaaaatt tcccgccaaa aaatcaccca ctttttaaaa
      901 taaacaggtt cgcgcatgat attaaactgc caatctagct ccctatggtc gctgtaagaa
      961 ggagtgatcg ctacgctctc acattttact tgcagcagtc gcctttaggc tccttcgcca
     1021 aaaataattc attagggact cctttgtggc gtcctgagag ctcgtctaag ccgttctaag
     1081 acaagattcg ataaaaacta tgtccttcgt tctaaagcgc cttacaggcg gtcagggcaa
     1141 gccctaacaa ccccaaggca ataaaaaact taggcttatt tttgcttttt tattgtataa
     1201 ttgattgtcg cctatccgac agtcttgcga ctggaaggag gtgtgtttcg tgttcactaa
     1261 ctttatttgc tcccttcttg ttgggctttt aagcgctttg ttcagttact ggttgaataa
     1321 ccgaaacaag aagtaaaact taattaaggc gactcagccc acaaaaaaag ccccagagaa
     1381 tccgacctct ctggggattt cgtgtgtttc gtgttcactt atttgcaatc aaattataac
     1441 agaaataaat atttatttca aaaatatcct acaaaagtct aaaatccgta attatttctt
     1501 atatgcaaca tttacaaata aagtcgctct gagagctcgt atttttcgat ttaagacgtt
     1561 ttatttagtt tttggtgtat ttcattggtc caagctaaaa aaactcttag aagcgatttt
     1621 agagccattt aaacccactt aattatattc tccatgaaat tcgctccatg cgtcaaaagc
     1681 tgcgtctgaa attccgccgt cttcgttcac tagttgcatt aatttttcca acgatcgttt
     1741 tctttcgggt gtcatatttt tatattccaa caatttttcg tctgttaaat ttggttatcg
     1801 attacacgtg cagcactccc ccccgaattt taaagagcat aaggaaacgt gtacccactt
     1861 tctaaaaaaa gtgggtagat aggctgtgcc tatcacgttt ctcagaaaaa aatttgatat
     1921 cactttttcg ccatcgtggg tagcttatgt atagcatttt ggcaaaaaac agctatacaa
     1981 atatagtatt tgtatggtat acataaatta tacataattg caaaaggaga ttttttatgg
     2041 acattttagt tcggaatatt gatccaaagt ttgttgcaac aatagacaaa cgttgccaag
     2101 aactcacgaa taaaacagga gaaaaatgga gtcgaaataa atatctaaaa gttttaatcg
     2161 aaagtgattt tgaccgtcaa cttacgactt acaaaaaaga ccaattcgat ttattgctcg
     2221 ataaatttat tgctatccaa acagaaaata ctcaaatatt aagcgagtat gtctacacaa
     2281 ataatcaaat cattgaaatg cttatcggag gaatgatata accatgagaa aaacttctgt
     2341 ccaaaaacat gttactttgt accctagtca tgttgacgtt ttagattcca tcatcgaaaa
     2401 aaatccagat atcaataact actcggaagc cattcgcttt ttatgtatga gttatgaacc
     2461 cgataccaac acgaccacta ccagcaatca ggcaaaacta aatgctatgg gaaaagaaat
     2521 ttccatcatg actgaaatca tcgcagaatt tgctgattta tatctaaaag atacaggcgt
     2581 tcttattggc acaaattcta aagtatatca agaagtgaaa gaaaaaatcg aagctaaact
     2641 aaatccatca aatcgaaatc gaccaaagaa tcatcaaaaa gaaaccagtc aaacagtcac
     2701 ttctagaaat ttcgacccaa caaattttct aaaacgataa aaaacagata ggagttattc
     2761 ctatctgttt tttatttaga agccccatac aatatgctag aatgtactag caatcttagt
     2821 cggttgtaag ggacttgtct acttgcttaa tgtaaggggg gcgagttctt ttttatttat
     2881 tcttctaacc agttacgcag agaaatcttt ggcaaatctg acgttaggct tgaaaaagta
     2941 aattctaatt tggcgatttt attgcccttt tttgctttta tttttgtgac tttaagatca
     3001 ttgaagtatt tttttagttc tcgtaatgca ggatttaata ctttttgatc aatgttaccc
     3061 atttgatagt attctgggat atccattaag ctcctaaact cttcaatacg aactacataa
     3121 taaccagtag aacggtactg cataagcagt cggaatagag tttttgcata cgtagaacgc
     3181 aacgacgtga aagcagata
//
LOCUS       DAFEPQ010000145         3126 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.152, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000145
VERSION     DAFEPQ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.152"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(<1..245)
                     /locus_tag="LP543_002737"
     CDS             complement(<1..245)
                     /locus_tag="LP543_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL2322848.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCG"
     gene            complement(235..564)
                     /locus_tag="LP543_002738"
     CDS             complement(235..564)
                     /locus_tag="LP543_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL2322849.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(641..913)
                     /locus_tag="LP543_002739"
     CDS             complement(641..913)
                     /locus_tag="LP543_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBL2322850.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(913..1176)
                     /locus_tag="LP543_002740"
     CDS             complement(913..1176)
                     /locus_tag="LP543_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL2322851.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(1282..1698)
                     /locus_tag="LP543_002741"
     CDS             complement(1282..1698)
                     /locus_tag="LP543_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322852.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            complement(1704..2267)
                     /locus_tag="LP543_002742"
                     /pseudo
     CDS             complement(1704..2267)
                     /locus_tag="LP543_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            2347..3126
                     /locus_tag="LP543_002743"
                     /pseudo
     CDS             2347..3126
                     /locus_tag="LP543_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1003 a          596 c          467 g         1060 t
ORIGIN      
        1 ccacataaca atactggaat aaccttgttt tctataatct cgtaagctac tctatatgtt
       61 gtgcccgcat atttgaaccc actagaccaa atacctgata aatccccagt tttttgttca
      121 cccttatatg gatcaatcgc aatttcatca taaattaaat tcaaaaattt ttctttcaat
      181 ggcttctctt ttagcttttt taaatactta atgacatgtt ttgaaggaac gatttcataa
      241 gccaatttct gttgccaact cctctttagt cattacttta gaattagtaa gtgtatcttc
      301 agcaatatct ctaaaggaag aatgcatttt gttttttcta ttcttaaatt ctacaagaat
      361 ctcatcttta tcgtagcctt ctgcaattat atcagacaaa atatcttcgc taaaatcata
      421 aaattctttt tgtggttcaa caaattcata aagaatacca ttctctacca actttgcttc
      481 aacttcgact ccattaggaa cattaaaatc tttaggtact gtcaacatga ttgaattgcc
      541 ttgttttctt gtttttgtaa tcatttaaat ccctcatttc taacagtatg taattactta
      601 gtaaatacat tataccacga gataatgtat ttacaagtaa ttactgtgac tcgtaatgcg
      661 tccttgctga ataaatttca accacgtgtt catcttcatc taccttataa acaacccggt
      721 gttgtctatt caatcttcta gaatacctac cttcatatgt aggttttaac ttctcaaatg
      781 attgggtagg gagataggga tcttccttta aagtctgaat aaccttctca aattgaggtt
      841 tcaaattaga ctgtttgatt ttctttaaat cagctttagc cgagttttta atcatcacag
      901 aatagttatt cattataagt tatcccaatc aatttcatca acattagtaa atccactatc
      961 atctttttct cgcttacgaa caacacccaa agtgcctatt tgctcaagta atagcgtttc
     1021 tttgattgct ttcaaatcat ccaaaccaat aagcaccgca ttattttcac tgcgctcact
     1081 aatgatttca atttccgtat gattctcatt tacatctttc aacaacttat aaaaattttt
     1141 ccgtgctgag ctaggattta agactgtttt tgccatatct actccccctc taatttatat
     1201 ctccattcta acgtacttaa ttaagtacgt aaattcgatt gttttgttta aattggagta
     1261 ccaaaacagc attgtaaagt gttatccgtt tcctttattt ttattaacaa gttttatcac
     1321 aggcttcata tatttgatta taacttcatc ttctacgtag atatctgtta tcattacgac
     1381 atctgtacta tttcgattga gtactttcac ttgatctcca gcattgattt tccctctaaa
     1441 tcttttagga atgaaccaac tatacatatg attagcatca ccaaaatgtg cttttatcgc
     1501 attctcagga tataacttat tcaatagttc attcttttta attttcaaaa cttcaaatct
     1561 atacttatca tcatctgcta atctaaataa atcttctaaa cgcatgtttt ctaactgtaa
     1621 gttttgaaaa tcttccggta taaattttcg gttctgttgc gaagtttgaa aatcaaacgc
     1681 gccctctctg aactccaaat atcttaggct gttattccca ttaatacctt gatttcagta
     1741 gacaccgaaa agctgaagag cgttccattt cttcggttct ttttatatat tcctcgaatg
     1801 gtctccatgc ccttaatcgt ggaagaggct gtacggagac tttgataaaa tttattccgt
     1861 cgtttaatag gtcgatggtc ttgttctatt aaattgttaa gatacttcac agttcggtgc
     1921 tctgtcttag tatataaacc cacactctgt aactttctaa aggcggagcc aagagaaggt
     1981 gctttatcgg tcacaattgc tttcggctca ccaaactgtt tatggagtcg ttttaagaaa
     2041 gcataggctg cttgcgtatc ccgtttcttt cgtaaccaga tatctaaggt taagccgtcc
     2101 gcatcaattg cacgataaag ataatgccaa cgtcccttaa ttttgatata ggtttcgtcc
     2161 attttccatg aatagaagga ttgtctattt ttcttcttcc aaagataata gaggactttg
     2221 ctgtactctt gcacccaacg ataaatcgta gtatgacaaa catttattct atggataggc
     2281 tacaattaac tagacagaaa aattaaggtg tgtagactag aagaaaatat accaggagga
     2341 atttttatgt ctaagagaac acgaagaact ttttcacaag aattcaagca acaaatcgtc
     2401 aatctttact tagctggaaa gccacgtgta gaaatcattc gagaatatga actaacggct
     2461 tcagcatttg acaaatgggt aaagcaatct aaaacgagtg gttcattcaa agaaaaagat
     2521 aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca gcaattagaa
     2581 atggaaaatg atattttaaa gcaagcagcg ctgatattcg gacgaagaga caagtaatcg
     2641 atgcgaataa gcatctttac cctatatcag cgatgtgcag aatattaggt ctatcacgtc
     2701 agtcctatta ttatcaatca aaaccaaaga aagacgaatc agaacttgaa gaagtagtcg
     2761 ctgaagaatt tatccgcagc cgaaaggcct acggctcaag aaaaataaaa aaagccttat
     2821 caaaacgagg cattcagctc agccgacgaa aaattagtag aatcatgaaa aatagaggat
     2881 taaaatcgag ctatactgtt gcttatttta aagtacatca ttctacttgc aatgaagcca
     2941 aaacgacaaa cgtattgaat cgtaaattct taagagacaa cccattagaa gcgatcgtaa
     3001 cagacttgac ttatgtacga gtcgggaaaa aattgaatta tgtctgtttc attttggatc
     3061 tgttcaatcg agaaattctc ggctattctt gtggagaaca taaagatgcc gttctagtaa
     3121 aaaaag
//
LOCUS       DAFEPQ010000146         2917 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.154, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000146
VERSION     DAFEPQ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.154"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            204..1172
                     /locus_tag="LP543_002744"
     CDS             204..1172
                     /locus_tag="LP543_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBL2322853.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1165..2196
                     /gene="vanA"
                     /locus_tag="LP543_002745"
     CDS             1165..2196
                     /gene="vanA"
                     /locus_tag="LP543_002745"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBL2322854.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2202..2810
                     /locus_tag="LP543_002746"
     CDS             2202..2810
                     /locus_tag="LP543_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBL2322855.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          863 a          559 c          708 g          787 t
ORIGIN      
        1 aattttttag gaaaatctca aggttatctt tactttttct taggaaatta acaatttaat
       61 attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga gccgttttcg
      121 ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt tggtgccttt
      181 cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat ggatgtgagc
      241 aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg gcaacgataa
      301 ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa tgtatcagtg
      361 tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga gccggtgtga
      421 aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct gctaagagaa
      481 tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat tatactatga
      541 tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg gaaaaacatg
      601 atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt ggtgtggtgg
      661 gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga tgtaaagtgt
      721 tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat gagttgctgc
      781 aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac tatattatca
      841 gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact gggcgcggtc
      901 cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg ggcggtgccg
      961 cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc caaaaaccaa
     1021 ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc acaccgcata
     1081 cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt aaaaactgtt
     1141 tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact gtttgggggt
     1201 tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc taacattaat
     1261 aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg gaaaatgtgc
     1321 gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact ctcgccggat
     1381 aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa ccatgttgat
     1441 gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca aggtctgttt
     1501 gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat ttgtatggac
     1561 aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc cttttgggtt
     1621 attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt tgttaagccg
     1681 gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga attggactac
     1741 gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc tgtttcgggc
     1801 tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagctgttgg cgaggtggac
     1861 caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc ggaaaaaggc
     1921 tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg aggacggata
     1981 caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc ccgtgtggat
     2041 atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct gcccggtttc
     2101 acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact tcccgaactg
     2161 attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata ggatttactt
     2221 ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact tgggataatt
     2281 tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca tacgagttgg
     2341 ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga ttgcttctat
     2401 gggacggtta ccgtcctaat cgtgctgtaa actgttttat gcaatgggct gcacagccgg
     2461 aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag atgatttcaa
     2521 aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat cttacgcttt
     2581 atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt atggatgaac
     2641 gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc agacgtttgc
     2701 gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg cactatgtat
     2761 taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa acttttaacc
     2821 gttgcacgga caaactatat aagctaactc tttcggcagg aaacccgacg tatgtaactg
     2881 gttcttaggg aatttatata tagtagatag tattgaa
//
LOCUS       DAFEPQ010000147         2863 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.155, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000147
VERSION     DAFEPQ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.155"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            6..2378
                     /locus_tag="LP543_002747"
     CDS             6..2378
                     /locus_tag="LP543_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322856.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2439..>2863
                     /locus_tag="LP543_002748"
     CDS             2439..>2863
                     /locus_tag="LP543_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322857.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYA"
BASE COUNT         1144 a          456 c          621 g          642 t
ORIGIN      
        1 cggacatggc agaagaaaaa gaaccactag acaatagtcg cttgggaaaa tccaaaagaa
       61 aattagtacg attgcaaaat gaattaaacg aacaaattga gaagatgttt gaacaccaga
      121 gaaagaccaa cggtcagcca atgaacgata agagaaatgg gcattcttgg tttaggcaac
      181 aagaacgttt agaaaacaaa gtgcatagtc tgagagaaga gatcaagcaa caagaaaaac
      241 aggtagaaaa acttgaaaga caagaagagc taaaggaaat gggctataac aagtacggcg
      301 gcctggatat gacaatcgaa aatattccaa gaatcaaaga agaaatcgaa cgctttgaaa
      361 aaggtgaatc gactttttca gccgctacga tccgaaaata ccaacggaaa ttggaaacat
      421 tagaacagct gaaagaaaga tcagaaaaag gaaaagaaaa tcttcttcct gaagtacaag
      481 caattattga cagcggacga gtgacgcaat ggaaaaaaaa tccaaccatt tattttttga
      541 aagactatcg aaaagttgct ttagaactga atgaaaacgg gggatttgag gaatcaaaaa
      601 aataccaagc cacgtcggaa gaggaaaaag cagctgttcg tgaattactt gaagaggctg
      661 cgccaactga ttctttgaag gagaaagaaa acgatcattt ctatcgagta gaattcaacg
      721 aaaatgatcc agaaaaagga ttgaaaagtt atggtgggga gatcgtaaca ttagaattga
      781 tcgatcaact caaaacgttg gataaccaat acgccgcaga agcaggttat tataaattct
      841 attttgaaga agtgaagaac ggagaagtag tcgatcagat ccgaatggat ctaggggatg
      901 gcgtcaacag taatcaaccc atctatttag agttagcaaa attgtgcgaa caagtacctg
      961 aaaaggaaca aaaacaaacg gtcaaaaatg aacctgtttc gaaagaacaa atgcagtcga
     1021 aagaaacaaa aacaaaacaa aagggctatc aaaaacgaac ggcagaagag attaagcaag
     1081 aagtcaaatc cctttcgcaa caggcactgg atgcggtgaa aaagcatacc cattctcctg
     1141 aagacgtgaa agaacttttg aattttatgt ctcgttttcc agaacgatcc tttagaaatc
     1201 aattattgat tgaggaacaa tttccaggtg caacggcttg tttaggtcgt gctgcgttga
     1261 aaaaagaagg aatctatatc aaaaaagggg aacaagggaa caagatcttt gtcagaaaaa
     1321 cggtcaaagg attttatgag aaaggacgtg gctttgttcg agaaacagaa gctacaccag
     1381 aagataaaaa acgaatcgaa agtggtcaaa tcgaagtgat caaaaaacct tactacacga
     1441 ttgaaaaagt gtttgatatc acacaaacac agttaaaacc tgaagactat ccgaaaatct
     1501 ttccgaaccg tgtctttgat tttcaattgg ataaaaaagg acagtcagaa cttcaagctg
     1561 ggattcaagc ggtagccaac aatattggaa tacaaattcg agatatgaca gaaagtgaag
     1621 tctatcaaag agaattagga caagcacggg gggcgtatgt tcgtaacgaa tttactggcg
     1681 aagaagaaat cgtcatgaat actcgaaata ctcctacgca gcatttagct acaagtattc
     1741 atgaattagc ccatgcaaga atgcacaaat tttcagaatt agataccgca actaaagaat
     1801 tgcaggcaga aatgacttct tatattgtgt gcaaacattt tggaatggac acttcggaga
     1861 aagctattcc ttatattgca gcatggacga agaatggtca gacgttggat gataaggaag
     1921 cggcggaacg tgggaaaatc atgaatgatg tcagccgtgt cgcaaatgaa ttcattcaaa
     1981 ccatttctaa tgaaatcaat caatacagag aaagagaacc agagatccaa ccagcagaag
     2041 tgaagcagga gcataaaaat actgtagaaa cggcagagaa taagaatatt gtgcccgttg
     2101 gttcactttg gatcgacaag aaagacggga aagtaatgga agaagatacg ggtcgaacgt
     2161 tacatttgaa ggatgcggcg tttattgaag atccgcagca taatcaagtg attttaaaag
     2221 gaaaaagtta tggaaaagag attcaaacgg cgttaccttc agaagaattt gcgctaggaa
     2281 atccattccg attttcactt gaaacagtgt cagaagtggc caaacgaaaa aatgaaatct
     2341 attatgaaca acaattgtca caaaaagaac gcgtataaaa caagtaaaaa agactatctg
     2401 aaaaacggat agtctttttt cttttgggag gtttgaaaat gaccgaaaaa aaaatgagtc
     2461 ttattgatcg atgtaaacag atcgatatag tggattttgc tcgaaacaat ggaatggctg
     2521 tagtcaataa aggacgagac tatcgcttag aagaccatga ttcatttgtt tttgatcgaa
     2581 gaaaacaacg tttttattgg aacagtcaaa atattagtgg cgacattatt gaattagcta
     2641 aacttttttt catagacaaa gagatccaag actctaagca acaatttaaa gcagccacag
     2701 attttattct aaagaatgaa gacaaaacag aacgagtaga aaatcttcat tttgagacag
     2761 aaaagtacaa agatcacccc gttgattatc aaccactcac agaaaaagga aggaattatc
     2821 tgaaggaaga acgaaaacta ccagagtggc tgattgatta tgc
//
LOCUS       DAFEPQ010000148         2778 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.156, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000148
VERSION     DAFEPQ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.156"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..197
                     /locus_tag="LP543_002749"
     CDS             <1..197
                     /locus_tag="LP543_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322858.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="LP543_002750"
     CDS             314..811
                     /locus_tag="LP543_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBL2322859.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="LP543_002751"
     CDS             898..1290
                     /locus_tag="LP543_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL2322860.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..>2778
                     /locus_tag="LP543_002752"
     CDS             1274..>2778
                     /locus_tag="LP543_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL2322861.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT          839 a          518 c          596 g          825 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagc
//
LOCUS       DAFEPQ010000149         2719 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.157, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000149
VERSION     DAFEPQ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.157"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="LP543_002753"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="LP543_002753"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL2322862.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1065 a          420 c          556 g          678 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatca
//
LOCUS       DAFEPQ010000150         2686 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.158, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000150
VERSION     DAFEPQ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.158"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(<1..709)
                     /locus_tag="LP543_002754"
     CDS             complement(<1..709)
                     /locus_tag="LP543_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL2322863.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRIGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LIASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWITFFEY
                     LKERGLQGTELVISDAHK"
     gene            1383..2195
                     /locus_tag="LP543_002755"
     CDS             1383..2195
                     /locus_tag="LP543_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322864.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
BASE COUNT          865 a          411 c          451 g          959 t
ORIGIN      
        1 ctttgtgcgc atcagaaata acgagttctg taccttgtaa accgcgttct tttaggtatt
       61 caaaaaatgt tatccaggtc tcttcgcttt cgccactttg aatcatgaag ccgataattt
      121 cacggtcgcc atctttggtt attccaatcg ctatatgaca gctttttgag agtactcgat
      181 tttcttctcg tacttttata tagagtacat cggtcattaa gtaaggataa tttttttctg
      241 ataataaacg attctgccac tcgttaacca ttggttctag ctgttctgtt aagctagaaa
      301 cgaaggactt agagacggat ttaccacaaa gttcttccac aatttttgat actttacgag
      361 ttgaaacgcc tgatacatac atttccaaca ttgaagcaat gagggctttt tcgtttcgtt
      421 gataacgttc aaacactgtg ggtgaaaaat ggccatcacg tgttctgggt acttttaatt
      481 ctagcgtgcc tatacgtgtc gtaaagctgc gctcataata gccatttcgt tgactttgtc
      541 ggttttctgt tcgttcatat tcttttgctt gaatatattc tgttcgttga ttttccatta
      601 gttgattaaa taccgttgtt aaaatatttt tagaaacgtc atcctttaca gaatattcaa
      661 taatgctttg aatctcttcg cttttcagtg taaaatgtac ttgggtcatg taaaagtcct
      721 cctgggtatg tttttgtcgt taaaaacatt gtaccgtaaa aggactgtta catggccttt
      781 ttacttttac acaattataa ggactttatc gaaaaaaagc agttatttgt taaaatcatt
      841 gatattatta ataataagta ttttttgaaa ggggaaatat aaaaggtttt tgaaaataaa
      901 taaaaaatag tattacttgt tctattgcta gttattgcta gtctttattc ttttttgttt
      961 tttagggatg tacaaaagat ctcaaccaca ggagacattg cttttcactt ttctagaata
     1021 aaatgcttgt cctcagtatt ttctggtcca ataaatttta ccacatttaa tagctatggc
     1081 ggaggcgtga atttttttat ccatatctaa catctttcca gccgtaatat tttactggac
     1141 aacaaacaat ttgattctaa gttatatact tactatacgt tgggatactg aatatctgta
     1201 caattctagt gagctatcaa tatggacaaa agaatagatg cagccttctt attcagctgt
     1261 ttgtatagct tttatggatt gatatatatt agcgtactgc aatttcagaa tcaattgcgt
     1321 tgacgattgt accagttgtc ttgtattaca catatactct tctttatgaa agaaaaaact
     1381 gcgtgattct tttagctgtc agtatgagtt tgctcatcta tacccatatt ctctccacct
     1441 ttatgagtgt gattgttatt ggcttcttgt ttatcggctc tcttttctct aagcggaaag
     1501 agagaaaaga aatgtcgaat ctgatatcaa atatgataaa agctgttggt tggaccattc
     1561 ttttaacagc ttacgtttgg tatccgatgt tacaacaagt cctacatcaa aaaattaatc
     1621 gtccttttcg cacaaaactt caagaaaggg cattaaacat ttctgatagt ttaatcggtg
     1681 cagtaaataa tgatttagtt acatttacaa tgggaacaat tggtgtggtt tcatttattc
     1741 ttccactttt ctttaaaaaa aaatttgcca ataaagaaaa gattattaat ttttgtgcag
     1801 tgaccacttg gttcttatca acgaatcttt ttccatggga atttttacaa aaaacaccta
     1861 ttcaaatcat tcagtttcct tggagaatct tgggttttca agtgcttttt ggttcattga
     1921 tcttaattat agtgttttta aagtggaaaa caagcaacaa aaaaagtatg tgtagccttg
     1981 taggaatagt ccttcttatt tttacagtga ctgtagcaac agaagcgaat tattcgcaga
     2041 aaattcaatc gtataaaggt cgtttaatta tgaccaaaaa agatgttaca ctttatacaa
     2101 cctcgagaac tggaggtctt tatgactatg caccattaga tgcattgaaa tataaagatc
     2161 atttaaagaa acatgaagta aaagtctatg attgatggga gaatgcatct cataaagcac
     2221 ataattctaa aataatctat acagtaggag atgcgaatga gcaaaatatc acgttacctg
     2281 tatttaatta ctgggaacag ttatgaaagt aaatgggaaa aagagcacta tagaaaatga
     2341 caatggttta gtgtctatta aaggtacggg aaaaagaact atagttgagg tttcatctac
     2401 gtatccaact acgatgattc tatctttatt aattagttca ataactttta tattcttatt
     2461 tacacgttat ttaataagat gctgcggtta aatgaaaata gattagatag atattataaa
     2521 tatgttgatt ctattttcat agcaatggag aaaatacatt agcaatatat aaaagaaggt
     2581 atcaagagtc acggtgaatt tattccgaaa agtgaaaaga acaaatataa aatttaataa
     2641 aaatatagaa ctaaatagga aaacgtagac gtaattttct acgttt
//
LOCUS       DAFEPQ010000151         2650 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.159, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000151
VERSION     DAFEPQ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.159"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            36..197
                     /locus_tag="LP543_002756"
     CDS             36..197
                     /locus_tag="LP543_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322865.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
     gene            210..713
                     /locus_tag="LP543_002757"
     CDS             210..713
                     /locus_tag="LP543_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBL2322866.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            773..1162
                     /locus_tag="LP543_002758"
     CDS             773..1162
                     /locus_tag="LP543_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL2322867.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1149..>2650
                     /locus_tag="LP543_002759"
     CDS             1149..>2650
                     /locus_tag="LP543_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL2322868.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKG"
BASE COUNT          788 a          439 c          610 g          813 t
ORIGIN      
        1 aatggttttt aaccaatgcc caatccttag tcttactggc aattgtggtc attggtttgt
       61 ttttaggatt taagcgggag ttttccaaac tgattggctt tttggtgatt gctttgattg
      121 cggtcggatt ggtctttaat gcttctgggg tgaaggatgt cttactcagt ttgtttaacc
      181 gaattgtcgg tgcgtaaggg ggaagcacga tggaacagat gcgtgtttat attgcgaacc
      241 tcgggaaata taacgaaggt gaattggtgg gggcgtggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacgaatt gggttaaatg acgaatacga ggaatatgca attcatgatt
      361 atgaacttcc ctttgagatt gatgagtata cgccaattga ggaaattaat cggttgtgtg
      421 gcttagcgga ggaacttgaa ggaacgccag ttggtgaagt tgcttctgag attcaacatg
      481 ctttcttcaa ttcttttgag gagatggtag agcatgtgga tgatattatc tattatccag
      541 attgcgatga tatgagtgat gtagcatatt tcttgattgt agaagctggt gattttggag
      601 aagtaccaga acacttgaag acgtatattg attttgatgc gtgtggtaga gacttggaga
      661 ttggaggcaa ttatttggta acgagtcgag gtgtttttga gtatccatat taacaaaatt
      721 attgaagggt agttgatttc taaactatcc ttctttttaa aggaatgatt tgatgaagaa
      781 aataaaaagc tacaccagta tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt
      841 tcaactgcct tttcctgtga cttttaacca gatggcttgg tttgttttat ccttgctgtt
      901 tgtcatcgta tttgcccatg tgcctccact atctatgata gagggggcct ttttaaaata
      961 cttaggtata ccggtggcag ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa
     1021 accattggga tttttacgat cttttattag ctatcactta cgatttaagg tcactttcgc
     1081 aggaaagaaa gtcaaagaac agaagaaacg atgggacgaa cccattactc tggtaaggag
     1141 tgtgaactat gtacccgatt aagtatattg aaaataatct tgtctttaat caagagggag
     1201 aatgttttgc ctactatgag ttaatacctt acaattactc ctttttgtca cctgaacaga
     1261 aatatcaagt ccacgataac tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc
     1321 atgccttaca aattgccaca gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg
     1381 aaattaccgg gcgattaaca gaagtagcga aacaacgaat tgatttacag acagaggcat
     1441 tagtatccat gattggcgat agtcagattg attatcgctt ttttattggc ttcaaactga
     1501 ttgcgacaga tgaagaggtc aatctaaaga atctcaagaa atctttcttc tccggctttc
     1561 aagaatttgt ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg
     1621 aagaaattcg ccgatatacc aagcttgaaa aactaatgga aagtaaattg gctcgaagat
     1681 ttaaagtcag acgagtcaca ccgagtgatc tgatgtattt gattgaacat atctatggcg
     1741 aaaagggtac accatttgaa gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg
     1801 aaactttggt gaaacgttac gacttattac gtcccagtcg ttgtttgatt gaagaaaagc
     1861 cccggtactt atgcatggaa catgagaatc atgaatcata tgtagcgtac ttaacaatta
     1921 atacaattgt gggggaaatg gagtttccct cctcagaact tttctattac cagcaacaac
     1981 aatttacatt cccaattgac actagtatga atgtggagat tgtgacgaac aaaaaggcac
     2041 ttgctaccgt tcgcaataag aaaaaggaat taaaagactt ggacaaccat gcctatcaat
     2101 ctgacaatga aacaaattcc aatgtcttag atgcattgga ttccgtagat gaattggaaa
     2161 ctaccttgga tcagtccaaa gaatccatgt acaaactgag ctatgtggtt cgagtaagtg
     2221 ccgaatcagt agatgaacta aagcgccggt gtgatgaagt gctagatttt tatgatgata
     2281 ccaatgtaaa actagtccgg ccttttggcg atatgatggg gctacatgaa gaattcttac
     2341 cttctagtaa gcgatacatg aatgattata tccagtatgt tacgtctgat tttctcgccg
     2401 gacttggttt tggcgccact cagatgttag gggagttgga aggcatttac tttggttata
     2461 acgtagatac ggggcgcaat gtttacctga aaccggcatt ggcctcacaa ggggtcaagg
     2521 gttcagttac caatgcgcta gcagcggcat tcctcggttc actcggtggc ggaaagtcct
     2581 ttagtaacaa tctcttagtt tattacgccg tcctttttgg tggacaagct gtgattgtcg
     2641 atccaaaagg
//
LOCUS       DAFEPQ010000152         2642 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.160, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000152
VERSION     DAFEPQ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.160"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            702..2561
                     /gene="ltrA"
                     /locus_tag="LP543_002760"
     CDS             702..2561
                     /gene="ltrA"
                     /locus_tag="LP543_002760"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL2322869.1"
                     /translation="MNKVKTNKTLRHSEYYGMTQIFDELYVKSDKKEVFTELMTIIMS
                     DENIRLAYRNIKNNKGSYTASTDKKSIRDIKTLSEDTFLTEVKVLFRCYKPRAVRRVE
                     IPKPNGKTRPLGIPSIWDRLIQQCILQVLEPICEAKFHNYSYGFRPNRSTHHAIATCN
                     RLANRGKLHFVVDVDIKGFFDNVNHSKLLRQMWTIGIRDKWLLGIIRSMLNAPIVLPT
                     GKVEHPKKGVPQGGVLSPLLSNIILNELDWWVASQWQNTTIETEKIMDRRDKGKGIDK
                     TNQYRALRRTNLKEIYIVRYADDFKIFCRKRSHADKIFHATKMWLKERLSLEISEEKS
                     KIVNLKKGKSEYLGFELKLIKKGKKYVVESHMSKKSMTRVKIQLKKQLRKVARPKNHC
                     EQAKEIGLYNSMVIGIHQYYGIATCVNLDCSKIAYTLKPFINHKLPTKKHGKILNTFI
                     KSKYGKSKEMRWLNNVPIVPLNYVQFRLAVPLSEGTCKYTESGRSKIHSKLKLDLALL
                     LHMMRNSNYERSIEFVDNRISKYSAQKGKCAITGKFLEYEEIHCHHIVPVKQQGTDKY
                     SNLIIIHKNVHALVHAIEEKIIHKYLNVLNLTNEQIEKLNELRVKAGNSVLTV"
BASE COUNT         1021 a          421 c          505 g          695 t
ORIGIN      
        1 gcacttaaaa gaagaaaaca taagtttcct tttaaggaga actgatattt tgagcagtgg
       61 aatgatgggg taacgccctg aaacgctccc ttaatactcc gactggctgt ttaacatata
      121 gctgttagat ggtcagaagc tcggtgaagt cggctgaaag ttaccgtaaa tatgataaat
      181 tcatatacga aagttgttca gaggtgaatg atgtaaccta taggtcggag gtctataaaa
      241 tactcacggt gagaatgtaa gtaatactac tgacgaactt gcgaatgaaa gaatctatac
      301 acggactgta tagaaatgta caggattcta ccgtatcaac ggaacagaat gtatgttgtg
      361 gtttagtaag attattggtt atgaacaacc atgcaatgtt acaggcattg gaaatataca
      421 ggttcaaagc aagcacctaa aagtatatat aaagataaaa atatcgaaac atgggaagct
      481 ggcaatgtgg aggaaactat attccattga aacatttgtg acgaagaata ataagcacat
      541 ttatgccagt gaaagtggtg gcacagtacc aatgaggata tagaaatata tcggaggaat
      601 agccactagt caaaagaaat cgttaattaa atgatgtatt tcatagaatc gagtaagaca
      661 aaataaacac tacaacctaa gtaaacaaag gtagtggtgc tatgaataaa gtaaaaacaa
      721 ataaaacact tcgtcatagt gaatactatg gcatgacaca aatatttgat gaactgtatg
      781 taaaaagtga taaaaaagaa gtttttacag agctcatgac aattattatg agtgatgaaa
      841 atattcgact agcatacaga aatatcaaga ataacaaggg cagttataca gcgagtaccg
      901 ataagaaaag tattcgagac attaaaacac tgtcagaaga tacctttcta actgaggtta
      961 aagttctttt tcgctgttac aaaccacgag cagttagacg tgtagaaatt cccaaaccaa
     1021 atggaaaaac acgccctctg ggcattccat caatctggga taggttaata caacagtgta
     1081 ttttacaagt cttagaaccg atatgcgaag ctaaatttca caattatagt tatggatttc
     1141 gaccaaaccg ttccacacat catgcgattg ctacctgcaa tcgactggca aataggggga
     1201 agctacactt tgtagtagat gttgatatta aaggattctt tgataatgtc aatcactcta
     1261 aacttttgcg tcaaatgtgg acgattggaa tccgtgataa atggctatta ggtattattc
     1321 gatccatgct aaatgctcct atagtcctac caaccggtaa agtcgaacac cctaaaaaag
     1381 gtgtacctca aggtggtgtc ctatcccctt tactatctaa tatcatattg aatgaattag
     1441 attggtgggt tgcttcacaa tggcaaaata ccacgattga gacagaaaag attatggata
     1501 ggcgagataa aggcaaaggc attgataaga ctaatcagta tcgggcatta agacgaacaa
     1561 acttaaaaga aatatacatt gttcggtatg ctgacgactt caaaatcttt tgtagaaaac
     1621 gaagtcatgc agataaaata tttcacgcaa cgaaaatgtg gcttaaagaa cggttatcac
     1681 ttgaaattag cgaagaaaaa tccaaaattg tcaatctcaa aaaaggtaaa tcagaatatc
     1741 taggatttga attaaagcta atcaagaaag gtaaaaaata cgtagtcgaa agtcatatga
     1801 gcaaaaaatc aatgacaaga gttaaaatcc aattaaagaa acaattacgt aaagttgcca
     1861 gaccgaagaa tcattgcgaa caagcaaaag aaattggatt atataattct atggtgattg
     1921 gaatccatca atattatgga attgcaacct gtgtaaatct cgattgcagt aaaatagcct
     1981 atacactaaa accgttcatc aatcacaaac tgccgacaaa gaaacatgga aagatattaa
     2041 atacatttat caaatcgaag tatggaaaaa gtaaagaaat gcgatggttg aataatgtgc
     2101 caattgtacc gctcaactat gttcagttta gacttgctgt ccccctatca gagggaactt
     2161 gcaagtacac tgaaagtgga cgaagcaaga tacacagtaa attaaaatta gacctagccc
     2221 ttttacttca catgatgaga aatagtaatt atgaaagaag tattgaattt gtggataaca
     2281 gaatatctaa atattctgct caaaagggaa aatgtgctat cacgggtaaa tttctcgaat
     2341 acgaagaaat ccattgccac catatcgtac cagtaaaaca acagggtaca gacaaataca
     2401 gcaacctcat tattattcac aaaaatgttc acgcattagt ccatgctatc gaagaaaaaa
     2461 tcatacacaa atacctgaat gtattaaatc taaccaatga acaaatagaa aaactaaatg
     2521 aattacgtgt caaagctggt aatagtgttt taacagttta acttgaaaaa tatatcatac
     2581 ttaacattct tttgatggaa cgccgtatga agtgaaagtt tcatgtacgg tgtgaagcgg
     2641 gg
//
LOCUS       DAFEPQ010000153         2623 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.161, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000153
VERSION     DAFEPQ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.161"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="LP543_002761"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="LP543_002761"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL2322870.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          941 a          458 c          581 g          643 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaa
//
LOCUS       DAFEPQ010000154         2536 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.162, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000154
VERSION     DAFEPQ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.162"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            58..351
                     /locus_tag="LP543_002762"
     CDS             58..351
                     /locus_tag="LP543_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322871.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            348..572
                     /locus_tag="LP543_002763"
     CDS             348..572
                     /locus_tag="LP543_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBL2322872.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            569..1594
                     /locus_tag="LP543_002764"
     CDS             569..1594
                     /locus_tag="LP543_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL2322873.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNPYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            1784..1857
                     /locus_tag="LP543_002765"
     tRNA            1784..1857
                     /locus_tag="LP543_002765"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1818..1820,aa:Ile,seq:cat)
BASE COUNT          853 a          419 c          495 g          769 t
ORIGIN      
        1 aagaagagag cgcatcttaa ttgatgtgct ttttattttg attcaaggag ttgtcacatg
       61 attaatttag gggaatgggg agcgatagca ggatcaataa ccgctatcgt ttctttgatt
      121 ttattagtaa taaaaccaat tactgcatct ttctcgaaga ttactgagac tctttcaaaa
      181 gtaagtcaca atttagattt gctgactaaa gatttagaat cgagcaaatc agatcgattg
      241 atgattcatg aagaactaaa gaaacacgat gaaagattag atacacatgc agaaaaattg
      301 gtagaacaca cgcaacaaat taaaacttta tttagggaga gaagaaaatg aataataaaa
      361 cgttcgaagt actaaaatgg ttcgcactgg taattattcc cgcactagct actttcgtgg
      421 ggttagttgg taaagcgctc aattggcagt acacagatat ctgtgttgtc atcattactg
      481 gttttggcgc gtttttaggg agtgtgttgg gtgtatcaaa tcgaacctac aaaatgttct
      541 cggctgaaag cgaagaagga ggaaacaaat gaaaaagaaa attactatta ctgcgatgag
      601 cctgttaacg gctctttttt tattacccat taatacgttt gcttatacta ttaatgacga
      661 gtataattta gcgccgaatc aaggagactc cagattagca attcctaaca aaattatttt
      721 gcacgaaact ggaatagatg caccagcaag aaacgtagcc gccaacatga aaaataatta
      781 taacggaagc aatccttata ctacagatgt tattggtgac ggtgggattg tttaccgtgt
      841 gggtgagcaa ggatatgttt cgtggggagc tggtaacgct aatccttatg cgcctgtaca
      901 gattgaatta cagcgcacat atgataaagc attgtttgaa aaaaactatc gagcttatat
      961 tgaatataca agagatagtg caaaaaaata tggaattcca ttgactcttg atcaaggaac
     1021 ttctttattt acaaaaggaa tcatttctca tttgtgggtg acaaattatg tttgggggaa
     1081 ccacacagat ccatatggtt acttatcgca aatgggagtt agcaaagaaa agcttgctta
     1141 tgatttagct catggattta ccgatgaaaa tccaacaact tctgaaaaca agcctgtcat
     1201 tgatccaaca cgagctggtg cagctaatcc tacgctgaca gatggaacaa atcacgccca
     1261 cattgatcag tttggggaaa tcgaaaacgc aaacttgcat gtggctggat ggcacattgc
     1321 taactataaa tacgagtata ttttcattat ggactacaat actgggaaag aattagctcg
     1381 agtaagagct gatggaattt atagaccaga tgtaaaccaa gcttataata cttctggaaa
     1441 tgttggttat catgtatctt tcaatatgcg taattttcct agtaagaaag tctatgtaat
     1501 gatgcgggca acgaatgatc cagagggaaa cactaaaggc ggtgcgcaag atttccatga
     1561 caaacgttgg tatttaaata ttcctaaacg ataaaaatag ctcctcgttg aggagcagta
     1621 cataactata ttgacaacta taaaaattat tcgataaaat agtgatgtta tcgcatatct
     1681 tcactatcac ccataaatag tcacactcca agctatgcga taacaggttt gttgccacac
     1741 attctactgg ttgattgttt atggctttat gtggcaacaa ccagtaccct tagctcagtt
     1801 ggtcagagca gacggctcat aaccgtccgg tcgtaggttc gagtcctaca gggtacatta
     1861 acgtagccat ttgaatcgtt ctgtgttaga atttttttga agagtattat acaagctaaa
     1921 gcttttcttc attgccactc aaatgagtgg cttttttatg tatcctttta tggattaatg
     1981 aaaggatgtt tcacatagtt atacttctgt atatttgaaa agttttactt tgatttttaa
     2041 atagaaagac atttgggtta aattgtgaga taataataaa gaagagttta aagcgcaccc
     2101 caaaccactt ccccataagt gtgttacgct ttaaactctt ttatatttga agccattaaa
     2161 aagcatacca tatttttgaa aaaaagtgag aaaaaaggct tacaattgga gtggtagtta
     2221 attagtgact tatttttgat tttatagcac tgatactata aaatatagat atcatcatat
     2281 tacacaatct taatactaac ttaaaaaata tctcctttca caagtatggt gataaaattc
     2341 gttccgggct acctttttag gtagcctact ttaatcttta tacctttctg gatcaacgaa
     2401 agtatacttt atatagtcat aacgccgatg atcgctacgt gcgtccggca cgtcagtcat
     2461 gaatcggcta cactagacta gacagaaaaa ataaggtgtg tagaataata aaaaacacac
     2521 tggaggattt ttcatg
//
LOCUS       DAFEPQ010000155         2468 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.163, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000155
VERSION     DAFEPQ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.163"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(493..1785)
                     /locus_tag="LP543_002766"
     CDS             complement(493..1785)
                     /locus_tag="LP543_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL2322874.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(2059..2319)
                     /locus_tag="LP543_002767"
     CDS             complement(2059..2319)
                     /locus_tag="LP543_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322875.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          823 a          459 c          372 g          814 t
ORIGIN      
        1 ttatattata aattcacatg attatcgaat tttattaagt tagtttttct tgtaaatctc
       61 tgttcccaat gaaactcgag acacttattt taaagaggaa acaatgtggt atttaaatag
      121 ccctcttgta tgggctactc tgtgatactt attagccatt aaactatttt acccacatag
      181 catatatttt gaaatcgttc attccgtaaa ttcaactaaa tatgcctaaa aaaataaaaa
      241 atctaagata gaataagatt tattcagtct tagatggatt tatagcaact ttttcgtaac
      301 aactttaaaa aatacgaaaa gaaagagctg ttttactctg actacgttgc taaattgtcg
      361 tttaatcatt catttaaacc aaatcttttt ttatagaatt cgttttaatc ctcctgaatc
      421 caaaaactat gaaaaccgta attaaatagt taagagtttt taaccaccat aattctctcg
      481 gctattttga aatcagataa attttgatcg gacacatagt tgaataaacc taccgttgtt
      541 acggaaggta aatcgcatac ttttcattct agaggagaag gtctcgttga tcattaactg
      601 attaagcacc atagcccagt tttgaattcg accacctgac cacttcgcgt gtaattcttt
      661 cgtgcgtaaa taaagtagtt ttaggagtgc attctcatta gagaacgctc ctttttttgt
      721 gacttttctg aagctggagt ggacactttc aaccgcattg gtagtgtaca taatttttcg
      781 aatggcacta ccataatcaa atagttgttc aacatgtgca aagttccgtt tccagacatc
      841 tacagcacca gaataatgag accaccgatt ttgaaaactg tcaaaagcag catgtgcagc
      901 gtttagagaa gaagcaccgt agaacttttt catatctcgg cagacttcct tatagtcctt
      961 acttggaata tagcgcaatg catttcgaac aagatgaaca atacagcgct gaacaattac
     1021 cgatggaaag atcgcttttg cgccttcttc gaggccagaa acaccgtcca tcgaaatgaa
     1081 aaagacatct tcgacaccac gtgctttcag ttcatcaaat acttgcatcc agcgattttt
     1141 agattctgtt tgatttaacc ataatcctaa aatctcctta tttcctttga gatcatagcc
     1201 aagaatggtg tatacagcat attctttggc ttcataattt tctcgtaaag taacatacat
     1261 acaatcaacg aatagaaagg cataacactt tgctaggggg cgggcttgcc attcttccaa
     1321 ttcaggaagg acagcgtcag tgatatctga aatcatttca tgggaaatat caaagccata
     1381 aatagcttcg acggttgcgg caatatctcg ttgactcatt cctcgtgcat acatggaaag
     1441 aaccttccct tcgatgtcgg agacatctcg ttttctctta ggaattaact ctggttcaaa
     1501 ggaagcttcc cggtctctag gaacatcaat agctacttca ccaaaactgg ttttaagcgt
     1561 tttagttcca tagccatttc gacggttatc gtgttcctta ggctctttag aatgggcatc
     1621 ataacctaaa tgattattca attctccttg aagcattttt tcaaaaaggg gcccaaacac
     1681 atctttcaaa gcatcttgca tgtcatcgac agattcaggt tgataggcat tcagaatgga
     1741 ttcagctaac ttttcggcat caggatttct tttctttcta gccatcgtgt aatcacctct
     1801 tgatcttatt gtagaaaaag aaaaaccgcg aagcaaccac ctgcctagga ttaatggtta
     1861 cttacacggt ttatattaca ctctcatcat taaaaaagta tctttcgata ttactaaaca
     1921 atgaaattat ctatttgaaa tttaaatctg ataatatatt gagtgttcca atgagggctt
     1981 tgatcctatt atccatatct ttttcagttt taatattgat acacattata agaaaaagaa
     2041 acttttggtt aatttatttc atcccagtgc aaaatataaa aaagtaaaat agaccatagg
     2101 caatagatta acaattaata taatacaagc aaatattcta ttcattgtat ttctgctttc
     2161 aaaaagttct aatattgaaa gtataattcc acaaatgcct ataataagta tgggaataag
     2221 attagaaaat aacaccccga gccaatctgt gaaaattact atatttacta atatccctat
     2281 taacatacct atcatattta ttaaatcaat ttttttcatt agatatttct cctttattaa
     2341 atctgaagta cttatattaa aataattcta aactatttac ctcaaaaagt gcaataaatt
     2401 atttctgaac aataaaatga tagtcaaaac aaaaaaatat tgttaatcgt tcagagccat
     2461 ccgagagt
//
LOCUS       DAFEPQ010000156         2422 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.164, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000156
VERSION     DAFEPQ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.164"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..290
                     /locus_tag="LP543_002768"
     CDS             <1..290
                     /locus_tag="LP543_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322876.1"
                     /translation="ETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNK
                     NYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(803..876)
                     /locus_tag="LP543_002769"
     tRNA            complement(803..876)
                     /locus_tag="LP543_002769"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(840..842),aa:Ile,seq:cat)
     gene            complement(1065..2084)
                     /locus_tag="LP543_002770"
     CDS             complement(1065..2084)
                     /locus_tag="LP543_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBL2322877.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(2095..2301)
                     /locus_tag="LP543_002771"
     CDS             complement(2095..2301)
                     /locus_tag="LP543_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBL2322878.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          740 a          466 c          405 g          811 t
ORIGIN      
        1 atgagacaca attatggttt aaatttgacc cttcgaatag atttatcaaa gatttttata
       61 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
      121 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaaggatg
      181 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
      241 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa agtaggctac
      301 ctaaaaaggt agcccggaac gaattttatc accatacttg tgaaaggaga tattttttaa
      361 gttagtatta agattgtgta atatgatgat atctatattt tatagtatca gtgctataaa
      421 atcaaaaata agtcactaat taactaccac tccaattgta agcctttttt ctcacttttt
      481 ttcaaaaata tggtatgcct tttaatagct tcaaatataa aagagtttaa agcgtaacac
      541 acttatgggg aagtggcttg gggtgcgctt taaactcttc tttattatca tctcacaatt
      601 taacccaaat gtctttctat ttaaaaatca aagtaaaact tttcaaatat acagaaatat
      661 aactatgtga aacatccttt cattaatcca taaaaggata cataaaaaag ccactcattt
      721 gagtggcaat gaagaaaagc tttagcttgt ataatactct tcaaaaaatt ctaacacaga
      781 acgattcaaa tggctacgtt aatgtaccct gtaggactcg aacctacgac cggacggtta
      841 tgagccgtct gctctgacca actgagctaa gggtactggt tgttgccaca taaagccata
      901 aacaatcaac cagtagaatg tgtggcaaca aacctgttat cgcatagctt ggagtgtgac
      961 tattatgggt gatagtgaag atatgcgata acatcactat tttatcgaat gatttttata
     1021 gttgtcaata tagttatgta atgctcctca acgaggagct atttttatcg tttaggaata
     1081 tttaaatacc aacgtttgtc atggaaatct tgcgcaccgc ctttagtgtt tccctctgga
     1141 tcattcgttg cccgcatcat gacatagact ttcttattag ggaagttacg catgttaaaa
     1201 gatacatgat agccaacatt tccagaagta ttataagctt gatttacatc tgatctataa
     1261 attccatcag ctcttactcg agctaattct ttcccagtat tgtaatccat aatgaagata
     1321 tactcgtatt tatagttagc aatgtgccat ccagctacat gcaaatttgc attttcgatt
     1381 tctccaaact gatcaatgtg ggcgtaattc gttccatctg tcagtgtagg attagctgca
     1441 ccagctcgtg ttggatcaat gacaggcttg ttttcagaag ttgttggatt ttcatcggta
     1501 aatccatgag ctaaatcata tgctaatttt tctttactta cgcccatttc agaaagataa
     1561 ccgtaaggat ctgtatgatc gccccagatg ttttgtgtta cccataaatg cgatttgatt
     1621 cccggttggt tataaggagt gtccaacgtt aatggaatac catatttcat tgctgaatct
     1681 ctagccaatt caacgtatgc cttgtagttt ttctcaaacg ttgctttatc atgtgtgtgt
     1741 tgtaactcaa tctgcacagg actgttggca ttagcatacg aaccagcacc gtactgtaca
     1801 taaccaggtt gaccgacttg ataaacaatt ccgccgtctc ccacaatata agcagtataa
     1861 gcgctagtcc atgaacgttg catatactgc gcttcattgc gtcctgttgc tgtttcatta
     1921 gccgtttcat gcagtaaaat atactgatta tttgctactt gtgagctacc ttcatttgcg
     1981 cccaaattaa attcattgtt gatagtatag gcaaatccgt taattggcaa taaaaaaaga
     2041 gccgttaaca ggctcatcgc agtaatagta attttctttt tcatatctat cctcctattt
     2101 atctgatgaa attttcttat acgttcgatt tgatacacct aaaatactcc ctagaaatgc
     2161 tccaaaacct gtaataatca tcaccgttat atctgtgtac cgccagttaa caccttttcc
     2221 gacaagaccg acaaaagtcg ctaaagcggg gataactact atagcgatcc atttcaaaat
     2281 atcaaaggtt ctattgttca tactatccac ctcccttcat tggttgggct actttctttt
     2341 tcaatcgctt gttttcttct atcaatattt ctctttctga aagcaaggca tcaatttgcc
     2401 cgtccagttg ttcaatttta tc
//
LOCUS       DAFEPQ010000157         2400 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.166, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000157
VERSION     DAFEPQ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.166"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            209..2071
                     /locus_tag="LP543_002772"
     CDS             209..2071
                     /locus_tag="LP543_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL2322879.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSARAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2071..>2400
                     /locus_tag="LP543_002773"
     CDS             2071..>2400
                     /locus_tag="LP543_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL2322880.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          354 c          466 g          714 t
ORIGIN      
        1 taaaaaattt gaaaaagcgt ttgattattt atattacgac ttagtgcaat aaaaaacgct
       61 ttttaacagt acatgtagtc gtctttataa tagataaact ctgtaatatt agttaaaaag
      121 ctataagttg cacttagtta agtgcaaaca tttcatattg tcgttagttt agaaaacgct
      181 tatattagca atgtaaggag agatgataat gcagcagcta acaaaaagag aaaggcagct
      241 tttgatttac ttggtggaaa tgtctgaacc ggtaacaatc aaaaagttgg ctgaaaaaga
      301 aaatgttagt gttcggacga taaaatacga tctagatgat ataagagagt ggctgaatga
      361 gcggaagcaa gtcctatata gcaaacgtag tcagggaatt tggatgaata ttagtagctc
      421 tgatcgaatc aaattaaaaa gcgaattgat ggatgttaat cgttttgaaa tatttgcaga
      481 tcaaagtctc cgaatcaaac gtttactaat tatgctttta gtaacaaaag aagctattac
      541 tggaatgaaa atggccaacg cattagatgt cagcaaagat acgatcatga atgatttaga
      601 tgtgctggaa aataattttc ttaaggaagg attgaccttg aatcgtcagg cgcgaaaggg
      661 tttttggatc ataggagaag aacgtctagt tcgattaacg attgaagaga ttcttcaaaa
      721 agagttcacg gattatgaca tttataaatt aatgtcattg ttattgaatg gcggtgagac
      781 tgaatacttt gaaatgtatt ctgctactgc tacacctatc caagaggtat ttaatcaagt
      841 aatcattcga atgcgacact tactggaatt tgaaaatcta gagaaactga attacgcgga
      901 attactaaat attctgattc gagtgacaat tgctaccgtt cgcttacgca aagaggctac
      961 gattggtcgt tatcaattgg ttgctgaaca agaattagac aaaaacgatc tttcttatcg
     1021 cgtcatgcag caggtctttg attattatca gttgccactg ttcgaggatg aatatcatta
     1081 tatctatagt gatacattcg acacgattca ccagcaagac gtcatgaaac tagttgagaa
     1141 tgtgatttat aaagtaagta aagcaatcaa ccattctttt tatcaagatc cacaattgtt
     1201 gacgaatctg tatgctcatt tatccatgcg attatcgaga aagaaaaaat ttgtcaatga
     1261 atataatcct tttaaagaag atatcaagca gaaatacccg gaattatttg atgcgatcaa
     1321 gcaagcagtt cgacaagaaa taagtgaaca aaatattctg atcaatgatt ctttcgtggc
     1381 atatattgcc ttacactttt tggtttccta cgaaagagaa gaagatctac gaagcgctag
     1441 agccgtctac atttgttcaa cagggttggg tgtgactagt ctgatcaagc aaaagatcaa
     1501 tgaagaatta aataatatca ctatcgtggc atttgcttca gtattgaatg cgcaagaaat
     1561 gattgctacc aaagatcctg atttggtgat tagtattttt ccaattaatg gcatcgatcg
     1621 tcctttcatc aaagtccatc cattacccac tgaacaggac ttgaaaaaaa ttcaacaaat
     1681 aataaaaaaa atacttgctt cttccaatca gcaggaaagg cataacttga atcttagaga
     1741 aacgtccatc aataaagata ctttagaaga ctttagcaag gaattgattt tgaaagctta
     1801 tacgatttat gaacgtttac ttgtcatttt ggaagcagaa ttgatccaag aatatcgaga
     1861 agcttttttg cttcatgtat tcttaatggt tcatcgtatc acatttgatc aacaatatat
     1921 ggcagaaggt cgtcaagtag accagattat tttagacaaa ggaacactgg taggaaaaat
     1981 cgaagcagtt tttgcagaaa ataatttagg aatcaatcag tcagaaatca gtgcattatt
     2041 ctcatatata aaagaaggag cgtcaagtta gtgaaaatgt cagacgaggc tttaaaaatt
     2101 atcgaggaaa gtcaaaatcg ggaaaagtta aaacaagtga ttgatctaat tgaagagcga
     2161 ttagtagaac atgctattgt accaaccgag ttgcagtgga ccattctaat taatcacttg
     2221 aatgagatgc tgaaacgttc tcaaagaaag gaaacgattc ccgtagtcga tcgggatttg
     2281 ttcaaagaag tatccaaaga agccataact atttctgatg aaatagttag aagtatcggt
     2341 aatttaaccg atgatgaaat atatgttttg tcgattcatt ttgaaacagc aaaaaataat
//
LOCUS       DAFEPQ010000158         2349 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.168, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000158
VERSION     DAFEPQ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.168"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            253..1551
                     /locus_tag="LP543_002774"
     CDS             253..1551
                     /locus_tag="LP543_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBL2322881.1"
                     /translation="MKHTHKIILAVTSVCTVIILGVSIFFVTQAATNNKLENTSQTTQ
                     HSSSSSKPVEDKQTTKQLDQAKQLAASYHYDEAIALLEKDDAKEAQQLLATLKKEKES
                     LVKWEDPTKISHVFFHSLIVDPAKAFHTQQAQGYKDYMVTISEFNKTIDQLYKNNYVL
                     VNLNGLVKKGTDGKLTFTGVSLPEGKKPLILSQDDVSYYEYMDNSGFPSKLIVDKQNQ
                     IKNIYIDNKKETVGDYDMVPLIDSFIKKHPDFSYQGAKGTLALTGYNGVLGYRTSKSE
                     YGDNEKTHKEIEAAKKVADQLKEDGWSFASHTWGHLNMTQASLADIQQDNERWQNEVA
                     PILGKTNILIYPFGADISDWQPYSEANQKFAYLKQQGFDIFCNVDASTPAWGQLGTDY
                     YRNARINIDGIRFEADLKGENPILDHFINVKEMYDQKDRG"
     gene            2198..2349
                     /locus_tag="LP543_002775"
                     /pseudo
     CDS             2198..2349
                     /locus_tag="LP543_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          836 a          400 c          429 g          684 t
ORIGIN      
        1 ttttagtttt tcttggaaat aaaaacagcg aaaatccgaa cattttcacc tctctcactt
       61 tataactttt acaaaatagt acttctcata aaaagcgctc tagttaaaga aaataagaga
      121 aaaatcaaaa aaaacaaaag tttcttttac acttgtttct atattcgtta caatggttat
      181 gggaagtttt taaaaaggtt aagaaaagct tacgggggtt tttgaaagca aaaagtagta
      241 ggggacgata aaatgaaaca tacacacaaa atcatattgg ctgtcacttc tgtatgcact
      301 gttattattc taggtgtaag tatctttttt gttacacaag cagcaacaaa caataaatta
      361 gaaaacacta gtcaaacaac tcaacactca agcagcagct caaaacctgt tgaagataaa
      421 caaacgacga aacagcttga tcaagcaaaa cagcttgctg caagttatca ttatgatgaa
      481 gccatcgcct tacttgaaaa agacgacgcc aaagaagcgc aacaattgtt agcaactttg
      541 aaaaaagaaa aagaatcctt ggtaaaatgg gaagacccga ccaaaatctc acacgtcttc
      601 tttcatagtc tgatcgttga tccagctaag gcttttcata cccagcaagc acaagggtat
      661 aaagattata tggtcactat ttccgaattc aataaaacca ttgatcagtt atataaaaat
      721 aactatgttc ttgttaatct aaatggatta gtcaaaaaag gaacagatgg aaaactgacc
      781 tttacaggtg tttctcttcc agaaggaaag aaaccgttaa ttctttcgca agacgatgtc
      841 agctattatg aatacatgga caattcagga tttccaagta agttgattgt cgataaacag
      901 aaccaaatca agaatattta tatcgacaat aagaaggaaa ctgttggtga ctatgatatg
      961 gttccattga tcgattcatt cattaaaaaa catcccgatt tttcttatca aggcgccaaa
     1021 ggaacattag ctttaacagg ctataatggt gtattaggtt accgtacatc aaaatcagaa
     1081 tatggtgata acgaaaaaac acacaaagaa atcgaagcag ccaaaaaagt tgctgatcaa
     1141 ttaaaggaag atggttggag ttttgcttct catacttggg gacacttgaa tatgacacaa
     1201 gcttctttag ccgatatcca acaagataat gaacgttggc aaaatgaagt tgcacctatt
     1261 ttaggcaaaa caaatatttt gatctaccca tttggcgctg acatcagcga ttggcaacct
     1321 tattctgaag caaatcaaaa atttgcttac ttgaaacaac aaggatttga tatcttctgc
     1381 aatgtcgatg cctcaactcc tgcatgggga caactaggaa ctgactacta ccgtaacgcc
     1441 agaatcaata ttgacggtat ccgtttcgaa gctgacctaa aaggagaaaa tccaatactt
     1501 gatcacttta tcaacgtcaa agaaatgtat gaccaaaaag atcgaggata gaggttgtga
     1561 aagaagcgtc cagctccgag caaataagaa ctagatttct aaaatagttt ctcatatttt
     1621 ggaaatctag cgcttattgc tgaggagttg cttcttgaac accgtgaata gaggttgtaa
     1681 aaagagcgtt ttgacttatt gttgaaggtc agctctttga acaccgcgtg ttaggttgtg
     1741 aaaaggctgg tctgcatcaa gcaataagaa tgaacagaga aaaacagctt cttgaatacc
     1801 gcagataggt tgtaaaaaaa ccgttttgac ctgacgataa ccagtaaaac agaggtgaga
     1861 ccattgctaa aagtagcagt ggtctcatct ctttttttgc acaagtcata tttgattgtt
     1921 tcgttatttt aatcaggata aagttctttt tttcatgcta aagagtgatt ctttcttctc
     1981 gattttcact ttcttcgttt aacttttggt aaatacttct gtcagaaaaa gctctaggta
     2041 gacacacgaa agcagataaa aagcctaact tttgcaacag tttatgctgt cgttcaaagt
     2101 taggctttta cagtttataa atagggttct gttgcaaagt tttccaaaaa atctatttta
     2161 gtgtaaaatt gagaaaaaag acagagagga cagagtaatg aatcatttta aaggcaaaca
     2221 attcaaaaaa gacgtcatta ttgtcgctgt tggttactac ctgcgttaca atctaagcta
     2281 tcgttaagtt caggaattgt tatatgatcg tggaataaat gtttgtcata ctacgattta
     2341 tcgttgggt
//
LOCUS       DAFEPQ010000159         2328 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.169, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000159
VERSION     DAFEPQ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.169"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            20..271
                     /locus_tag="LP543_002776"
     CDS             20..271
                     /locus_tag="LP543_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2322882.1"
                     /translation="MTVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHA
                     GMNYAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            485..1180
                     /gene="vanR"
                     /locus_tag="LP543_002777"
     CDS             485..1180
                     /gene="vanR"
                     /locus_tag="LP543_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBL2322883.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1158..2312
                     /locus_tag="LP543_002778"
     CDS             1158..2312
                     /locus_tag="LP543_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBL2322884.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          792 a          421 c          498 g          617 t
ORIGIN      
        1 tccatacagc caattcttaa ttactgtaat ggctggtgtt aaccaattag agcgagatct
       61 tattcggatg agacaacgtg aagggattga attggctaag aaagaaggaa agtttaaagg
      121 tcgattaaag aagtatcata aaaatcacgc aggaatgaat tatgcggtaa agctatataa
      181 agaaggaaat atgactgtaa atcaaatttg tgaaattact aatgtatcta gggcttcatt
      241 atacaggaaa ttatcagaag tgaataatta gccattctgt attccgctaa tgggcaatat
      301 ttttaaagaa gaaaaggaaa ctataaaata ttaacagcct cctagcgatg ccgaaaagcc
      361 ctttgataaa aaaagaatca tcatcttaag aaattcttag tcatttatta tgtaaatgct
      421 tataaattcg gccctataat ctgataaatt attaagggca aacttatgtg aaagggtgat
      481 aactatgagc gataaaatac ttattgtgga tgatgaacat gaaattgccg atttggttga
      541 attatactta aaaaacgaga attatacggt tttcaaatac tataccgcca aagaagcatt
      601 ggaatgtata gacaagtctg agattgacct tgccatattg gacatcatgc ttcccggcac
      661 aagcggcctt actatctgtc aaaaaataag ggacaagcac acctatccga ttatcatgct
      721 gaccgggaaa gatacagagg tagataaaat tacagggtta acaatcggcg cggatgatta
      781 tataacgaag ccctttcgcc cactggagtt aattgctcgg gtaaaggccc agttgcgccg
      841 atacaaaaaa ttcagtggag taaaggagca gaacgaaaat gttatcgtcc actccggcct
      901 tgtcattaat gttaacaccc atgagtgtta tctgaacgag aagcagttat cccttactcc
      961 caccgagttt tcaatactgc gaatcctctg tgaaaacaag gggaatgtgg ttagctccga
     1021 gctgctattt catgagatat ggggcgacga atatttcagc aagagcaaca acaccatcac
     1081 cgtgcatatc cggcatttgc gcgaaaaaat gaacgacacc attgataatc cgaaatatat
     1141 aaaaacggta tggggggttg gttataaaat tgaaaaataa aaaaaacgac tattccaaac
     1201 tagaacgaaa actttacatg tatatcgttg caattgttgt ggtagcaatt gtattcgtgt
     1261 tgtatattcg ttcaatgatc cgagggaaac ttggggattg gatcttaagt attttggaaa
     1321 acaaatatga cttaaatcac ctggacgcga tgaaattata tcaatattcc atacggaaca
     1381 atatagatat ctttatttat gtggcgattg tcattagtat tcttattcta tgtcgcgtca
     1441 tgctttcaaa attcgcaaaa tactttgacg agataaatac cggcattgat gtacttattc
     1501 agaacgaaga taaacaaatt gagctttctg cggaaatgga tgttatggaa caaaagctca
     1561 acacattaaa acggactctg gaaaagcgag agcaggatgc aaagctggcc gaacaaagaa
     1621 aaaatgacgt tgttatgtac ttggcgcacg atattaaaac gccccttaca tccattatcg
     1681 gttatttgag cctgcttgac gaggctccag acatgccggt agatcaaaag gcaaagtatg
     1741 tgcatatcac gttggacaaa gcgtatcgac tcgaacagct aatcgacgag ttttttgaga
     1801 ttacacggta taacctacaa acgataacgc taacaaaaac gcacatagac ctatactata
     1861 tgctggtgca gatgaccgat gaattttatc ctcagctttc cgcacatgga aaacaggcgg
     1921 ttattcacgc ccccgaggat ctgaccgtgt ccggcgaccc tgataaactc gcgagagtct
     1981 ttaacaacat tttgaaaaac gccgctgcat acagtgagga taacagcatc attgacatta
     2041 ccgcgggcct ctccggggat gtggtgtcaa tcgaattcaa gaacactgga agcatcccaa
     2101 aagataagct agctgccata tttgaaaagt tctataggct ggacaatgct cgttcttccg
     2161 atacgggtgg cgcgggactt ggattggcga ttgcaaaaga aattattgtt cagcatggag
     2221 ggcagattta cgcggaaagc aatgataact atacgacgtt tagggtagag cttccagcga
     2281 tgccagactt ggttgataaa aggaggtcct aagagatgta tataattt
//
LOCUS       DAFEPQ010000160         2265 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.170, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000160
VERSION     DAFEPQ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.170"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            1148..1702
                     /locus_tag="LP543_002779"
     CDS             1148..1702
                     /locus_tag="LP543_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL2322885.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            1956..>2265
                     /locus_tag="LP543_002780"
     CDS             1956..>2265
                     /locus_tag="LP543_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322886.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     L"
BASE COUNT          879 a          249 c          350 g          787 t
ORIGIN      
        1 gttttccttt tcatatatat taaatattac ggaaatttat ataaactaat ttagtgtttt
       61 gtttgtaatt cctaattttg ctccactttt agataatttt taggtctatt taactcatca
      121 gacattctat gctcattttc tccaaatttg tttcaactct ttcgtactta attttgcgta
      181 tttgaaaatt cttcttttag agtactttta ttacgatact agtatgatat atatctatag
      241 caaacagtta aaatcttcct aaaataagcg aatagcattc ctaaaaccta taaaattttt
      301 agatgaagcg acaagtggac aattaataaa ctacttgaac atgaaattat ggatcacctt
      361 tcaaatttag aaaatggaat tcttatactt attgcgcaac atttattgat tgcaaaatta
      421 tgtgacaaaa agaactagta gaagaaggaa taatggcttg ataataaagt actacctatt
      481 aaaggtagta ctttatttaa tttagtataa attaacatac tactatattt taagtttcat
      541 attatataaa ttaataatat actaccattt tttggtagta taatttaaag gtaataagtt
      601 attcttttat tactatgttt gggttttata gcaaggaaag tttaataaga gtttggacct
      661 agcaaacaat attaatgagg tcattaaatc ttattatctt tagtgatata acgaaatatc
      721 tacacagaca aaattatgat cttgcgtaaa gttataaaat attaataaaa atattttatt
      781 aatgaaaagc ttggtagcac ggttattcat atgctaattt atttcacttt atagtaaggg
      841 gagttcttag tatattgtcc aaatttaaat ttcatataat ttaattatgt aaattagcta
      901 ttctaagaac ttctacggat aactgcccgt ctaaaattta attaagagct tagaatcttc
      961 atataagtaa ggttaatttt tttgtacaaa atagtcggaa tattctgagt tactgaaatg
     1021 atataaaatc ataaactaaa ccccttgatt ttcttcactt ttaacagagt atatatataa
     1081 gttcatgatt atggtaaaat atatatataa tcatgaactc agaatatttt taagaaggga
     1141 aacaatagtg agctataatg tacaaccgtt aaggacaaat gaagagatac aagattttca
     1201 gttttggcta agaagaacaa gtaatccaga aagagatagt tttttatttt tgtttggaat
     1261 caataatggg ttacgtatgt cagatattat aggattaaaa gttggagaca tacgaggtaa
     1321 aagtaagcct attattgttg aacataaaac aggtaagcga aaaccgattt ttatagataa
     1381 tttgagagaa gagattttat tatatactga aggtaaagaa gaaaatgatt ggttatttcc
     1441 aagtcggcaa caaggtcgac atataacaag ggatcgtgtc tatcagatat atgcagatat
     1501 agcagaaaaa ttaggaagag atgatatagg aacacataca ttaagaaaaa cttttgggta
     1561 tcactactac aagaaaacta gagacattgc taccttaatg tttattttta atcatagctc
     1621 gcaagcaatt acaaaaaggt acattggtat aactgaagat gaaatagggg catcgttacg
     1681 tggttttaaa ttgggaggct aattgagata ttaggatata ttttttgtgt ataatgaaaa
     1741 cttagaaggt gagattaatg aagataactg aattttcgga agtatttaaa acgtttagta
     1801 acatttacaa tagaatacca gagatagaac aaaatgttgt agttgataat gattattata
     1861 tagagaatga ttttaataaa ccaagaaaat atgacaacct atacaatttt atacacaata
     1921 gaacaacaat aaataacaac agtaacactc gagttttgat taattcagca tattttcata
     1981 ttaaatattt aaagaaatat gagtatgaag aagttgtatt agattatggg ctaaatagac
     2041 agtcattttt aatttatgca ataaataacg gaaatttgcc aagcaataaa aagaacataa
     2101 ttattgaaac atatgcctat ctggaggggg gatctaaaaa actaattaag tcatgtaata
     2161 tagagtatca attatcagta ggagaaatta aatgtgtttt cgaagaagat ttagaacttg
     2221 acttaaaaaa aatttttgcc agcattcatg attatatagg tttga
//
LOCUS       DAFEPQ010000161         2254 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.171, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000161
VERSION     DAFEPQ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.171"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..1548
                     /locus_tag="LP543_002781"
     CDS             <1..1548
                     /locus_tag="LP543_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322887.1"
                     /translation="SIDFGDYDYSENPNLMPYITEPWVGPLLGNGHTVKDSVKRVITH
                     TKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTYTLSVTMSVGSDWTGETNTI
                     GVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGITKDNVTSITNCYVEIFSLNS
                     EYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIADPTVVFPHKTSEYMVYGRRN
                     TENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMKPTEGLANTWELTFTVTKQH
                     IDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNISEYKYRGTGMRDSNNPKDY
                     VWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTK
                     ANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGKSS
                     GGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYT
                     FAITYTI"
     gene            1565..1714
                     /locus_tag="LP543_002782"
     CDS             1565..1714
                     /locus_tag="LP543_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322888.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1711..2157
                     /locus_tag="LP543_002783"
     CDS             1711..2157
                     /locus_tag="LP543_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322889.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            2159..>2254
                     /locus_tag="LP543_002784"
     CDS             2159..>2254
                     /locus_tag="LP543_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBL2322890.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEE"
BASE COUNT          799 a          386 c          467 g          602 t
ORIGIN      
        1 agcatcgact tcggggacta tgattattcg gagaacccga atttaatgcc atatattaca
       61 gaaccttggg ttggaccttt gttaggtaat ggacatacag ttaaagatag tgttaaaagg
      121 gttataactc atactaaaac tagaacagct aattccggcg acatattatc gcttggctta
      181 ggtattccgt gtacagcgga agctaataat aggtatctta taaccacttt acgaccatct
      241 acgacataca cattaagcgt tactatgagt gtcggttcag attggactgg agagaccaat
      301 actatagggg ttaggcttag atatttaaat gaacaaggtg gtatagaact gcccattaat
      361 gcattaatac cagcaaatgt agaacgcgat aaaatggtca ctcatacatt tactggtata
      421 acaaaagata atgtaactag cataacaaac tgttatgttg aaatattctc acttaatagt
      481 gaatataaag gaacggttag tgtcagctat gacgttaagt taaaagcaca ctaccctaac
      541 ttactagatg gcccttactg gttaggtaaa gttcctttag gcgagaatat tgctgaccct
      601 acagttgtct ttccacacaa aactagtgaa tatatggttt atggtagacg caatacagaa
      661 aactatatcg ctgaccaaac atataccata tcaatgaaag caactaaact aacagttcaa
      721 tcgtttgccg tgtatattgc tgctggccgc gttaaagtag gagacatgaa gcctactgag
      781 gggttagcta atacatggga gttaactttt actgtaacta agcaacatat agatagtggg
      841 gtaactaatt atctggaaat ataccaatac ccgtcagcta caaaaggagc tgttcaaatt
      901 gagtggctca aactagaaaa aggcaatact cgaaccccga atattagtga gtataaatat
      961 cgtggtactg gtatgcgtga ttcaaacaat ccaaaagatt atgtttggga tctagcacca
     1021 gaatatgtcg aagataattt ggccacagat attaaaattt ctgaaattac tggtaaagca
     1081 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
     1141 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
     1201 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
     1261 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
     1321 tattttgtgg ctttaaacgg aaaaagcagt ggtggaaatg attcaacgat actaacaatt
     1381 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
     1441 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
     1501 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1561 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1621 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1681 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1741 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1801 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1861 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1921 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1981 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     2041 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     2101 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     2161 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     2221 aatggttttc gttccgttgt gcattactga agaa
//
LOCUS       DAFEPQ010000162         2192 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.172, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000162
VERSION     DAFEPQ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.172"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(563..766)
                     /locus_tag="LP543_002785"
     CDS             complement(563..766)
                     /locus_tag="LP543_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322891.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(1225..2190)
                     /locus_tag="LP543_002786"
     CDS             complement(1225..2190)
                     /locus_tag="LP543_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBL2322892.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          631 a          506 c          346 g          709 t
ORIGIN      
        1 aattttgttc ctcctcaaag aaaaagagac ttttggaaag aatttccaga gttcacgtaa
       61 ccctaaaaat tcatatccaa aaatctctag cttgaagaaa cctaaaaata ttcgtataat
      121 gaacctgagt tacggagcta ggtgaagtag tttccgtaaa tagtcgagag tgttagcgca
      181 ctgctcggct tttattttgc tcttttttct cgttttcttt gattatagca ctcctcctaa
      241 aaaaataaaa agacctcagg gaatttcatt ccccgaaccc cttgagcctc ctcctgccga
      301 aatggaaagg gcgcacccgc tgtttatttc attcagctat cggctttcac aatctaacac
      361 gatcgttccg ctaccagtcg ccttacaggc tccattttcg ctcctctaac gtgttttaac
      421 gattgtgccg ataactaaac gaaataaacg ctaaaaccgc tcagaacgca aaatatgcca
      481 ttttagagct attgtagaaa atgtagtttg aaatgcaatt ttcttctttc agctttttat
      541 ttcccacttg taaatctgct aattattttc taccagacaa accattgatt ttttggctat
      601 cagttaactc acgattatta tgagaaatgc ttgtattctt tgttcccttt ttgaacgaaa
      661 tactaaaccc taaggtgaac ccctccttcg cgctgtcgct aataaaaaga gtgtaaacct
      721 ttctgttact ctttgacaaa gagtaacatt ttcctaattg tgacatggct gtcacaattc
      781 caaacgctct ccgagggttt tcgctgtcac aagtgacaac gaaaaaagga gccgaaagcg
      841 tgtcacgctc tcaactcctt ttacgcttta ttttgcttca cgcaaaataa tcgcttatct
      901 taaacattga cgtaaattta ctctcactta tctgactttt ttactcggta tcactccgag
      961 taagccgtca tattcattgg gataatctcg tctgcttcgc tctaattttc cagagtgagg
     1021 ggtaactcct tcccccgaat taccccaaag agtgacactg ggatatccct aaatttatta
     1081 taaaaagcca agtctctttg cgcaactgtt ttatccaagt cggcatttgc ggattcgtta
     1141 gcgttttttc gctcttgctg caaacgcacg acccaccatg ccgacttgtg ctgaaaataa
     1201 aaaagtggac aaaaattcat ccactcattc ttccgtcacg tccaacccca aaagccacgt
     1261 atccaattct tttttcaaaa catctgccga ttccgaaaat gtctctcgta aatgattaac
     1321 taactcatca ctttggcttt cttttttcaa caaatttcga tatttatatt ttgtatgacg
     1381 agccaagcca gcccataaat tttgatctga aatcaatcct gcaatcataa ttctatcagt
     1441 atgctttgta tcagtcggca aaaaatgagg acttgcgaaa ctgtctaaac tttcatgaac
     1501 catcgcatac caatcagtag cttttccacg ccgtaactgc aattccagtc gccaccaagt
     1561 ttttatttcc ggtggtacaa ttttccgttt ggtttcttgt tccaattttt tgttgtacat
     1621 ccttatttgt cgctcactgg cacgactacc ccaatatgcc gtttccagct tcccagaccg
     1681 cccatagatt ggtttgaatg aaacagggtc aaccacacga tattgcgtaa taaaatcatc
     1741 aggcacatcg ataatgtcgc aagcaatatc agcacgtgag aaatgtggtt ctaaaaataa
     1801 atcatggata aagttcttca tcgaacccgc caaaaattgg ctaattttat ttgggttaaa
     1861 gtcaattcgc ccttgccctt cttgatattt caaaatttca atgtaagcaa tattctcatc
     1921 atgcttatcc acaatttgcc aaccatttag accaacagat ttaacataac cattattttc
     1981 attcagtcgc attaactgtt caaagtctaa aatcaataca tcattttgcg tgtgataata
     2041 aatattctct ttgagttttc caacaatggt aattcggtca attgaccact gtattttaag
     2101 gggggtggtc aaagtttgca cacttaaccg cctgttagat agggctgtta aattttccca
     2161 ctcatttatg atcgtgccac gtttactcaa at
//
LOCUS       DAFEPQ010000163         2190 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.173, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000163
VERSION     DAFEPQ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.173"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            353..1081
                     /locus_tag="LP543_002787"
     CDS             353..1081
                     /locus_tag="LP543_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBL2322893.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNRSIILNDP
                     NWCKQANLREISMPSAWEETKGSKDVKVAVIDSGIDYQHEDLSGNVDESLGYDFFDDD
                     TDPMDTMGHGHILPGLLVLKQTTN"
     gene            1111..>2190
                     /locus_tag="LP543_002788"
     CDS             1111..>2190
                     /locus_tag="LP543_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBL2322894.1"
                     /translation="MVPIRVFGSSGGTSLAILTEAINYASQKGFPIINFSIGSVVYSS
                     TEKAIKNYPGLFVAAAGNNGIDMTDKPFYPASLDIPNIITVGNSSGYERSSDSNYRKT
                     GVDLFARGFRVFSTFLGSKYTYMSGTSMATPHVTGSAALALSLDPDLSTDELKKIILN
                     SVDSFYELSDLCLTGGRLNTNNVVRQVTGKLTASNITLRLNSEWSDEIAKEMCHVHWI
                     DQSGNDLTDQVVVLKNEVDTSNFGDYEVVFASPDLTDQISVTVTVPRLRKLTLGKETA
                     TIDIGSAWDSVIAIDTFQVKAIDDFGNDVTNQVQVTINADIDTINSIQAVIKFEVPEL
                     GLAQIGRLNITAKNSTINGLNEVSIE"
BASE COUNT          769 a          289 c          470 g          662 t
ORIGIN      
        1 cgtggagaaa taataagaac taccagtcag ataaaacaag ataccgaaga ctcactgcgc
       61 aaagggtggg gaatgaataa taaagttaga taataatcaa agaagaacta agtaactacc
      121 tttaaacggt agttacttag ttcttctttg atttgcgtga aatagagggg atttattttt
      181 ctggttacgt gattaaaaaa tccaaaaaat gagcatagga acggttatga ttttagggtg
      241 tgttttaatg agatattgtg atttcattaa caagtgtatg ataatctgtt gttgagttag
      301 taaataagta atagagctaa ctctaaaata aaactattag gaggagatgt aggtgaaaaa
      361 gtttaaaaag aaaatatatt taggattggt tgttggggtg agtatgtttg ggttcatggc
      421 tcatactaaa caagtaactg cagatactaa ccaaaaagaa aggattatga gccaattaga
      481 tgactctcaa atagagtatg tacccaatga gctaattgtc agtttaaagg ctacacaaag
      541 aatttcaaca gagaaaaata aaccgcaaaa tttattgggt gtaaaagtta tggtggaaaa
      601 agatttgact cttaggacaa atgaaagcga ttataataag cgagatcaag tattattagt
      661 gaaattgaac gaagaaatta gtgtggaaga ggcagtaaga acatttaatg cttctgatat
      721 ggtcaattat gcagagccaa actatgtggt tgaactagag ccgttgaatg atgttgagga
      781 gccaaataca actgattctt taggaaatcg atcaattatt cttaacgatc ctaattggtg
      841 taaacaagct aatttaagag agattagcat gccatccgct tgggaagaaa ccaagggttc
      901 taaggatgtg aaggtagcgg tcattgattc aggaattgat tatcaacatg aagatctttc
      961 tggaaatgta gatgaaagtt tgggatacga tttttttgac gatgatacag atcctatgga
     1021 tactatggga catggacaca tattgccggg attattggtg ctcaaacaaa caacgaatta
     1081 ggtgttgctg gaataaattg ggatgttacg atggtaccta tacgtgtatt tggtagtagt
     1141 ggaggtacca gtttggcaat cttaactgaa gctattaatt atgcatccca aaaagggttt
     1201 ccaattatca attttagtat aggtagtgtt gtttatagca gtacagaaaa agcaattaaa
     1261 aactatcctg gattattcgt agcagcagct ggaaataatg ggatagatat gactgataaa
     1321 cctttttacc cagcttcttt ggatatacct aatattatta ctgtaggaaa ttctagtggt
     1381 tatgaacgaa gttctgattc aaattatagg aaaacaggtg ttgatttatt cgctagaggg
     1441 tttcgtgttt ttagtacatt tctaggaagt aaatacacgt atatgtcagg tacctcgatg
     1501 gcaacacctc atgtcactgg tagtgctgca ttagcgttgt cactagatcc tgatctatct
     1561 actgatgaat taaaaaaaat cattttaaat agtgttgatt cattttatga actatctgat
     1621 ttatgtctta cagggggaag actaaatacg aataatgtcg tacgacaagt aacgggtaaa
     1681 cttactgcat ctaatatcac attaagatta aatagtgaat ggtcagatga aattgctaaa
     1741 gagatgtgtc acgttcactg gattgaccag tcaggaaatg atttgacgga tcaagtggtt
     1801 gttctaaaaa acgaagtgga tacatctaac tttggcgatt acgaagtagt ttttgcatca
     1861 cctgatttaa cagatcaaat ctcagttaca gtgaccgtac ctcgattacg taaacttacg
     1921 ctaggaaaag aaacagcaac cattgatatt ggttccgcat gggatagtgt catagccatt
     1981 gatacttttc aagtgaaagc tatagatgat tttgggaatg atgttacaaa tcaagtacag
     2041 gttactatta atgctgatat agatacaatt aattcaattc aagcagtgat taagtttgaa
     2101 gttccagaat taggcttagc ccaaattggt agactgaata ttactgcaaa aaatagtaca
     2161 atcaatggat tgaatgaagt tagcatcgag
//
LOCUS       DAFEPQ010000164         2063 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.174, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000164
VERSION     DAFEPQ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.174"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(141..416)
                     /locus_tag="LP543_002789"
     CDS             complement(141..416)
                     /locus_tag="LP543_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBL2322895.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(659..1969)
                     /gene="der"
                     /locus_tag="LP543_002790"
     CDS             complement(659..1969)
                     /gene="der"
                     /locus_tag="LP543_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBL2322896.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
BASE COUNT          591 a          450 c          345 g          677 t
ORIGIN      
        1 aaaaattaaa taaaatacat gtgttttcta tgtatgacat acggattcgg aatctctgtc
       61 tcttatttat tccttttctg ctaggaaata aaaagaaaag cacttgtagc cctagagcca
      121 caagcgcttt ttcaactgtc ttatttaaca gcgtctttta atgctttacc tggtttgaat
      181 gcaggtactt tgctagctgg aatttcgatt tcttcaccag tttgtgggtt acgacctttg
      241 cgagctgcgc gttcgcgaac ttcaaagtta ccaaaaccga ttaattgaac tttttcgccg
      301 ttagctaaag catcttgaat agttgaaaat acagcatcta ctgctgcagt tgcatctttt
      361 ttagttaaat cagtagctga agcaactttt tcgattaatt ctgctttgtt tgccatggat
      421 agattcacct cctccacaag agatatgata gaaatatcat ttagttaaaa ctttctgagt
      481 ggtccagaag tttaaaaaga aatattgatt aacttcaata ttctgttatc acgatatcat
      541 atgaaccgct taatagcaag gtttttacca attttcacat gcttttttta gtcatagaaa
      601 cgtaacaaac gaaactgtct ttttcttttg aaaaaaaaac gtcgatatgg taaaatgcct
      661 attttctcct acgagggata attcggatgg acgtcccttc aaaagtaaat gcttttctga
      721 tttgattttc taagaaacgt tcataagaaa aatgcatcaa ttcctcttca ttgacaaaaa
      781 cgacaaatgt aggtggtttg acagcgactt gtgtagcata aaaaatcttc aggcgttttc
      841 ctttatctgt tggtgttgga ttgattgcta cagcatccat aatgatatca ttcaacacag
      901 ccgaagagat acgaagattc tggttcatac ttacttgttc gatcatttca ggcagacgtt
      961 ccaaccgttg tttagtcact gcagaaacaa aaacgattgg agcgtaatca agataccgga
     1021 attcatcacg aatctcttgt tcgaaatcac gcattgtatt ggtttctttc tttacaagat
     1081 cccatttatt cacgacgata ataattccac gaccagcttc atgggcgtaa cccgctactc
     1141 gcttgtcttg ttcccgaatc ccttcttcag cattcagtac catcaaaacg atatcagaac
     1201 ggtcaatggc acgcattgca cgcatcacgc tgtatttttc agtattttcg tatacttttc
     1261 cgcgtttacg cataccagct gtatctatca ttaagaattt ctgaccactt tcagaaacga
     1321 agtgggtgtc gattgcgtct cgggtcgttc cttcaatatc cgaaacaatt acacgttctt
     1381 ctcctaaaat cgcattgatc aaagaggatt taccaacatt tgggcgacca atcaaactaa
     1441 acttaatcgt gtcgtcgtcc tcttcttcag tctcttcaga gaaatacttc actgcttcat
     1501 ctaaaatatc accaatccct agtccatggc ttcctgaaac aggataagga tcgcctagcc
     1561 ctaaggaata aaattcatag atatcgtttc tcatttctgg attatctact ttattgactg
     1621 ctaaaatcac tggtttatta cttctgtata aaattttcgc aaccatctca tctgcgtctg
     1681 tcacgccttc gcgtccgctg actacacaaa tgattacatc tgcttcttca atcgcgattt
     1741 ctgcttgatg cttgatctga tccataaaag gttcatcgcc aagatcaatt ccgcctgtgt
     1801 cgatcacgct gaattctcga cctaaccatt caccttttgc ataaatacga tcacgagtta
     1861 ctccaggagt atcttcgaca atcgagatac gctcaccagc gatgcggtta aaaatcgtag
     1921 acttaccgac gttcgggcgg ccaacgattg caattgttgg atttgccata ttatttcctc
     1981 ctcacaagtt tccaatctca cttagtttaa cgaatgcatt gccaactttc aagcatagat
     2041 tgtcaattaa acgaagtatt ttt
//
LOCUS       DAFEPQ010000165         1880 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.175, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000165
VERSION     DAFEPQ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.175"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(14..181)
                     /locus_tag="LP543_002791"
     CDS             complement(14..181)
                     /locus_tag="LP543_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBL2322897.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            complement(377..583)
                     /locus_tag="LP543_002792"
     CDS             complement(377..583)
                     /locus_tag="LP543_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322898.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            1037..1312
                     /locus_tag="LP543_002793"
     CDS             1037..1312
                     /locus_tag="LP543_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322899.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            complement(1309..1461)
                     /locus_tag="LP543_002794"
     CDS             complement(1309..1461)
                     /locus_tag="LP543_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322900.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(1770..>1880)
                     /locus_tag="LP543_002795"
     CDS             complement(1770..>1880)
                     /locus_tag="LP543_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBL2322901.1"
                     /translation="ISREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
BASE COUNT          588 a          367 c          291 g          634 t
ORIGIN      
        1 ttcgcaatct tatttaatgc ttagctattc ttttgccata caatggaata acttcattgt
       61 tttcctttcg tttatatgta tcgctcttta ttggtcttct atactcatat actttttcgc
      121 cattaccttt atgaacagtg attacttcat acttctgttc taagtattgt ggtctataca
      181 ttgttgtttc ctcctttgtg caaaaataaa aagaccactc aaagagtgat ctaatatgta
      241 atagtaactt gcacgatgca caaaacgcgt acgaaattgc gcacccctat atttttaaac
      301 cgccgatgcc tcggttgctt aaagtcgctg gagtgggatt gcaccactca cgagaactta
      361 ccaggctctc acgaggctac tcgccattta ccgctgcgtc ttctacttcc gccacagtga
      421 ccgaagcttg gtggtgtaca gatagcacac ttactacatt gccgtacgta gcaccgtata
      481 gcttcttaac gatctttttt cggtagtcgt aaccgtcata ggcatttaat gtcgctggca
      541 aggaatcgaa ccttgcatgg ttgccgaagc attgacctag cacacatgct tagcgtctac
      601 cctttccgcc acagtgacac tataaaatta ttcttggctg ctactatttt ttattttgcc
      661 catttttaaa tccaatcata tagacattaa gacagagcgc aaaaattgaa attattaatg
      721 gaatcatttc tcttcaccca cctttagtta tcgtgtgaat aattaaaaaa caatagacag
      781 caacaaaata acattgcttt gataatttgg tataaaccac tataaatttc ttttcttgca
      841 attattttta atatatgcta gattatcaac cgatatagtc actgcctgta ctagcggaaa
      901 ctagtgcagg ttttttgttc tatttactca aaagttatta cgataaatta atattgtgaa
      961 aataaatact aagcgtataa ttttatttat cagcgagtgg tccgctgaaa tataaaacaa
     1021 ggatgtgcaa gacaacatgt ataaacccta catgattagt tatgatctta ataacccagg
     1081 gcaaaagtat gataaagtat ttgaaataat aaaagaattt ggggcatata taaagctaca
     1141 aaaatctttt tggttagtta aaactaattt gaatccaaat caaatgtgtg aaaaattaaa
     1201 tacagtactt gataataacg attccttatt catctgtgaa ctgcaaaaaa attatcaagg
     1261 tagagctaca gaggaaaatt ggaaattcat taacgaacat attttctctt agtaaggatt
     1321 agatattttt tctctgttta aacaattgca aataccgtca atatctgaat tagaaacact
     1381 taccttttgc tccttgctac taccaatagc ttggagcaat tcttgtattt cttctggagt
     1441 gccttctact gataatttca tttttcttcc ctccaataca taaattaata gacagcagca
     1501 gttgaaagac gacaagaaca tgtttagttt tgtgagtgct gctgtctaat caaaataaac
     1561 agcaaccgat gaagaattta ggaggagttg aattcacatc cttttcttca taattagttg
     1621 ctgtctatct aagaagaagt ttaaaacgat gagggagatt gcctcccttc gtttattttg
     1681 tcgatcctgt ttcctaatct ttcgacacta ccataataac actggtaaat gtctaaaaac
     1741 cgccatcatt ccgccaaaaa accgccaaat tatttataag caattatttt tccgtgttta
     1801 tatgcttctg caaactctat tagagcttcc gacttcatcc gttgtatact tctttctgaa
     1861 taacccactt cacggctaat
//
LOCUS       DAFEPQ010000166         1701 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.176, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000166
VERSION     DAFEPQ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.176"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..255
                     /locus_tag="LP543_002796"
     CDS             <1..255
                     /locus_tag="LP543_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322902.1"
                     /translation="NFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSVI
                     SEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD"
     gene            258..411
                     /locus_tag="LP543_002797"
                     /pseudo
     CDS             258..411
                     /locus_tag="LP543_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            434..1639
                     /locus_tag="LP543_002798"
     CDS             434..1639
                     /locus_tag="LP543_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL2322903.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT          547 a          322 c          377 g          455 t
ORIGIN      
        1 aatttcagag tggctttagg tcgtatgtct gaaatgggct atggcatgat gtttggcagt
       61 gacgtacaaa aggatttctt cttaaagcga atcaaaggtc gtggctatgt tgatgtagga
      121 acaagtgtca tatcagagtt ttatactccc cttgtaccaa aaggatatga tttcttggag
      181 gaaattaaaa agttatccaa cagcagacag tccacgcagg cgacgtgcga agcggaagtc
      241 gcaggtgtgg actgatcttg ctggctggtg tggcaatagc cacgccagca cttaacccct
      301 cggtatctaa caggggggta caaatcgaca ggaaacagtc aaaaaaacat tagaaaatcc
      361 tttggttaca agggatttac aaaatttcag cgtatgtcaa atgggcttta aaagttgaca
      421 tacgcctttt tgattggagg gatttttact gaatgaacaa acttggttac agcatttaaa
      481 agaaaaacgc ttggcttatg gactatctca aaaccgttta gctgttgcga ctggtattac
      541 aaggcagtat ctaagcgata ttgaaacagg aaaagtcaag ccatcagagg atttacagca
      601 gtccctttgg gaagctctgg aacgcttcaa tcccgacgct ccccttgaaa tgctgtttga
      661 ttatgtaaga attcgctttc cgacaacaga cgtacagcag gtggtcgaaa acatcttaca
      721 actgaaactg tcctattttc ttcatgagga ctatggtttc tattcttatt cagagcatta
      781 tgctttaggc gacatattcg tcctttgctc ccatgaactg gacaaaggag ttctggtgga
      841 attgaaaggt cgtgggtgca gacaatttga aagctatctt ctggcacaac aaagaagctg
      901 gtatgagttc tttatggacg ttttggtggc tggcggtgtg atgaaacgcc ttgaccttgc
      961 cattaacgat aagacaggga ttttaaatat ccctgtactc actgaaaagt gccaacagga
     1021 agaatgtatc tccgtcttcc gcagttttaa aagctatcgc agtggcgaac tggtacgcaa
     1081 agaggaaaag gaatgtatgg gaaacaccct ctatatcggt tcattacaaa gtgaagttta
     1141 tttctgtatc tatgaaaagg actacgagca gtacaagaaa aatgatattc ccattgaaga
     1201 cgcagaagta aaaaaccgtt ttgagattcg attgaaaaat gagcgtgcct attatgcagt
     1261 ccgtgattta ctcgtctatg acaatccaga gcataccgcc tttaaaatta tcaatcggta
     1321 tatccgtttt gtagataaag acgattccaa acctcgttct gattggaaac tgaatgaaga
     1381 atgggcttgg tttattggga acaatcgtga acgattaaaa ctaaccacaa aaccagagcc
     1441 ttactccttc caaaggacgc tgaactggct atctcatcaa gttgccccga ccttaaaggt
     1501 tgcgattaaa cttgatgaaa tcaaccagac gcaggttgta aaagacattc tcgaccatgc
     1561 gaaactgaca gaccgacaca agcagatttt gaagcaacag tcagtaaaag aacaggacgt
     1621 gataacaaca aaaaaataac tcaaatacaa attcattgaa tatagagagg agaacatttt
     1681 tatgaatttt ggacaaaacc t
//
LOCUS       DAFEPQ010000167         1679 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.177, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000167
VERSION     DAFEPQ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.177"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(50..134)
                     /locus_tag="LP543_002799"
     tRNA            complement(50..134)
                     /locus_tag="LP543_002799"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(98..100),aa:Leu,seq:caa)
     gene            complement(152..223)
                     /locus_tag="LP543_002800"
     tRNA            complement(152..223)
                     /locus_tag="LP543_002800"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(189..191),aa:Gln,seq:ttg)
     gene            complement(233..305)
                     /locus_tag="LP543_002801"
     tRNA            complement(233..305)
                     /locus_tag="LP543_002801"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(270..272),aa:His,seq:gtg)
     gene            complement(367..451)
                     /locus_tag="LP543_002802"
     tRNA            complement(367..451)
                     /locus_tag="LP543_002802"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(415..417),aa:Leu,seq:caa)
     gene            complement(472..542)
                     /locus_tag="LP543_002803"
     tRNA            complement(472..542)
                     /locus_tag="LP543_002803"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(508..510),aa:Cys,seq:gca)
     gene            complement(588..659)
                     /locus_tag="LP543_002804"
     tRNA            complement(588..659)
                     /locus_tag="LP543_002804"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(625..627),aa:Gln,seq:ttg)
     gene            complement(671..743)
                     /locus_tag="LP543_002805"
     tRNA            complement(671..743)
                     /locus_tag="LP543_002805"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(708..710),aa:His,seq:gtg)
     gene            complement(746..819)
                     /locus_tag="LP543_002806"
     tRNA            complement(746..819)
                     /locus_tag="LP543_002806"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(785..787),aa:Trp,seq:cca)
     gene            complement(826..906)
                     /locus_tag="LP543_002807"
     tRNA            complement(826..906)
                     /locus_tag="LP543_002807"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(870..872),aa:Tyr,seq:gta)
     gene            complement(908..983)
                     /locus_tag="LP543_002808"
     tRNA            complement(908..983)
                     /locus_tag="LP543_002808"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(948..950),aa:Phe,seq:gaa)
     gene            complement(1019..1091)
                     /locus_tag="LP543_002809"
     tRNA            complement(1019..1091)
                     /locus_tag="LP543_002809"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1056..1058),aa:Asp,seq:gtc)
     gene            complement(1101..1173)
                     /locus_tag="LP543_002810"
     tRNA            complement(1101..1173)
                     /locus_tag="LP543_002810"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1138..1140),aa:Val,seq:tac)
     gene            complement(1189..1260)
                     /locus_tag="LP543_002811"
     tRNA            complement(1189..1260)
                     /locus_tag="LP543_002811"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1225..1227),aa:Glu,seq:ttc)
     gene            complement(1275..1364)
                     /locus_tag="LP543_002812"
     tRNA            complement(1275..1364)
                     /locus_tag="LP543_002812"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1328..1330),aa:Ser,seq:gga)
     gene            complement(1404..1477)
                     /locus_tag="LP543_002813"
     tRNA            complement(1404..1477)
                     /locus_tag="LP543_002813"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1441..1443),aa:Asn,seq:gtt)
     gene            complement(1487..1602)
                     /gene="rrf"
                     /locus_tag="LP543_002814"
     rRNA            complement(1487..1602)
                     /gene="rrf"
                     /locus_tag="LP543_002814"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          473 a          436 c          376 g          394 t
ORIGIN      
        1 aaaaaaaata tagcaactct ctataagaaa gttgctatat tgataattat accggcggcc
       61 ggggtcgaac cggcacgtcc tcaacggaca ctggattttg agtccagcgc gtctgccaat
      121 tccgccacgc cggcaaattt aaaaataata actggggtag ctggattcga accaacgcat
      181 gagggagtca aagtcccttg ccttaccgct tggctatacc ccaagaataa agggcgaatg
      241 atgggaatcg aacccacgaa tgccggagcc acaatccggt gcgttaacca cttcgccaca
      301 ttcgccatca taaaagatgt tatttaattt ctcaataaaa aactaagatg aagacatctt
      361 agtcaatacc ggcggccggg gtcgaaccgg cacgccctca acgggcactg gattttgagt
      421 ccagcgcgtc tgccaattcc gccacgccgg caagttgatt tattagtact aaggcggtaa
      481 ccggatttga accggtgatg aaggttttgc agacctctgc cttaccactt ggctataccg
      541 ccattatact attatcaatc tataaaataa agaataaatt atagaaactg gggtagctgg
      601 attcgaacca acgcatgagg gagtcaaagt cccttgcctt accgcttggc tataccccaa
      661 gaataaaaag ggcgaatgat gggaatcgaa cccacgaatg ccggagccac aatccggtgc
      721 gttaaccact tcgccacatt cgccatggca ggggcagcag gaattgaacc cacactaacg
      781 gttttggaga ccgctgttct acctttaaac tatgccccta taaaatggag gagagtggat
      841 tcgaaccact gaaccctaag gaacggattt acagtccgtc gcgtttagcc acttcgctac
      901 tcctccatgg tggcgcggga cagaatcgaa ctgccgacac acggagcttc aatccgttgc
      961 tctaccaact gagctaccgc gccatatttt aaaattatta attgaataac aagaacgacg
     1021 gttccgacgg gaatcgaacc cgcgatctcc tgcgtgacag gcaggcatgt taacccctac
     1081 accacggaac cagttcatta tggaggttaa cgggatcgaa ccgctgaccc tctgcttgta
     1141 aggcagatgc tctcccagct gagctaaacc tccaacattc aattaaaatg acccgtacgg
     1201 gattcgaacc cgtgttaccg ccgtgaaagg gcggtgtctt aaccgcttga ccaacgggcc
     1261 aggtaaattt gctacggaga gtaagggatt cgaacccttg agacagtttg gcaccatcta
     1321 catgatttcc aatcatgctc cttcggcctc tcggacaact ctccagagtg aaagaagctc
     1381 aatactcttt ctcaaaatga gaactccggc agtaggactc gaacctacga catcatgatt
     1441 aacagtcatg cgctactacc aactgagcta tgccggaata acatcagcgt ggcgacgtcc
     1501 tactctcaca aggggcaacc cctcactaca atcggcgcta agaagcttaa cttctgtgtt
     1561 cggcatggtt acaggtgtat ccttctcgct atcgccacca cactgtggtg ttatctttta
     1621 ttgagtaaat tttgttcact caaaactgga tttgaagtca cataagtttt tttccgagt
//
LOCUS       DAFEPQ010000168         1636 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.178, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000168
VERSION     DAFEPQ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.178"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(183..1502)
                     /locus_tag="LP543_002815"
     CDS             complement(183..1502)
                     /locus_tag="LP543_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HBL2322904.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDKRAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          485 a          300 c          325 g          526 t
ORIGIN      
        1 attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac
       61 taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa
      121 ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg
      181 gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga
      241 tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac taatttccca
      301 gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca
      361 aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg
      421 acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc agctttcgct
      481 tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga
      541 gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct
      601 tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg
      661 gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat
      721 ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt
      781 tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag
      841 atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt
      901 acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc
      961 aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc
     1021 gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc
     1081 gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca
     1141 tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt
     1201 tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa
     1261 tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca
     1321 tgagtaaaat acgctcgttt gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa
     1381 ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca
     1441 cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc
     1501 atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt
     1561 cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt
     1621 ttcacatggt tttaat
//
LOCUS       DAFEPQ010000169         1583 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.179, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000169
VERSION     DAFEPQ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.179"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            292..1479
                     /locus_tag="LP543_002816"
     CDS             292..1479
                     /locus_tag="LP543_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL2322905.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
BASE COUNT          467 a          296 c          315 g          505 t
ORIGIN      
        1 ttttaacctt ccggttttga ttggataacc ctatgaacca tcgttatttt aatttggtat
       61 taactcattt cttttgagat aatatattta ttggattgag aggctaagcc gttcccccct
      121 tgggccattg aacccgcact cgagagactg gctcctctct ctgataagtg gattgcgtat
      181 gagttagatg ttgaaagtcc ttactttact ccgtatctcg tacaaggtag tgacgctttt
      241 cagttgagtg gatttcaatc caaaattctt tttttgaaag gtcagatata catgtacttt
      301 ttaggcattg atatcggtaa acgcactcac gttgcttcta tcatgaacga ggaaggaaag
      361 gtattactaa aaggtttttc atttcctaac accactgaag gagcagagtc attaattgag
      421 cgaatggtcg actattccgg agcacccagt gactttgtta tagggatgga agccacgggg
      481 cactattggc tgtccatctt ttcatacctt cacgaatcag attatttgat tcacgttgtg
      541 aatccccttc aaacagacgg atggcgtaaa ggtacagaaa tacggaaacg aaaaaacgat
      601 attattgatt ctgttttgat tgctgactta atgcgctatg gctcatttgt cgaaacggtc
      661 ttatccgatg aaaatgtttt ttctttgaaa caactttctc gttaccgaac ttatttagta
      721 ggtacagcga gcgacttcaa aagaaaaatt atagccgtat tggatcaagt ctttccagaa
      781 tacgcgacga tttttaccaa gcagggcgtt ttcggaaaag cttctaaaga gttattgctg
      841 gcgtttagtg ccccgatcga cttagaaaac atttcctcag aaactttggc ccaatactta
      901 gctgagttaa gcaggaaccg aatcaaggca gaaaaagcgg accaattgaa gcaagcagct
      961 tctaactcct ttggagtaaa atttgctcaa gaagcgttca ccttccaact acgttcaatg
     1021 attgaacaat taaaattcat tgaaagccaa ataaaggaaa cggaaagaga aatcaagcac
     1081 ataatggaca ctttaaattc tgttattctc acaattcctg gaattggtcc tataaacggt
     1141 gcgactattc ttggtgagat cggagatatt caaaaatttt ctaacccgaa aaaactcgtt
     1201 gcttacgctg ggatagacgc ttccgtgaca caatcagggc aatatgaggc aacgcataat
     1261 gttatgagta aacgagggtc tccatatttg cgaaaagcct tgttttcggc ggctttagta
     1321 gcttctcaat gtgatcctgt ttttagtgcg ttttaccaga aaaaagtttc cgaaggaaaa
     1381 catcatctaa ctgccttagg tgcggtttct cgtaagcttt gttacgtgat tcatgctatt
     1441 ttgactaaaa acgagcctta tataattgtt tctgattagc tattgaacat tcaaaaaaac
     1501 taatggtaag aagcttattt gttgtaccct tttttcggaa ttatctgtta taaaatattt
     1561 tttgatttgg ctaattttta ctt
//
LOCUS       DAFEPQ010000170         1459 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.181, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000170
VERSION     DAFEPQ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.181"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..291
                     /locus_tag="LP543_002817"
     CDS             <1..291
                     /locus_tag="LP543_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HBL2322906.1"
                     /translation="KKSKERAELVKLEMAETGAGVRSSRHWKAAANIEFYYNEIQKGF
                     DQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLRRTANDKKTRSIQHGFI"
     gene            278..496
                     /locus_tag="LP543_002818"
     CDS             278..496
                     /locus_tag="LP543_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322907.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            493..912
                     /locus_tag="LP543_002819"
     CDS             493..912
                     /locus_tag="LP543_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HBL2322908.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            909..>1459
                     /locus_tag="LP543_002820"
     CDS             909..>1459
                     /locus_tag="LP543_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HBL2322909.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVV"
BASE COUNT          542 a          202 c          339 g          376 t
ORIGIN      
        1 aaaaaatcaa aagaacgagc tgaattagtt aagttagaaa tggctgaaac aggagcagga
       61 gtgcgaagta gcaggcattg gaaagcagca gcaaacattg aattttatta caacgaaatt
      121 caaaaagggt tcgatcagat gcgtgagctg gatcggcaaa caaattggag caagaaactt
      181 catcaagatc gtttcaaatt tgtagagaag tataaagaaa tattagaaga gtatttgagg
      241 aggacagcaa atgataaaaa aactcgttca attcagcatg gatttatatg atatcgaatc
      301 aggagcaaca ctatctgtgg aatcggacca tctaatcata aattttggtg gaaagcgcca
      361 gattattttg tgggtagttg atgatgtact gtttccagaa attgttcatg atttcgaaga
      421 atcaaaagcg gttgagtttg aaatagtgaa aaaagtaatg gaattgattg aaaaatacga
      481 ggaggacagc gaatgatacc gaagtttaga gcgtgggata aacgaaagaa cgtaatgaga
      541 gatgtagccg tcttgcattt tactaaaaac ggcaaaacaa actttattga atattggata
      601 aatcctaccg aattgaaatc atatcacgtg cgaaacatcg acctcatgca atccacagga
      661 atgaaagata agaatggtgt ggatatattt gaaggggata tagtattagt cagcgtgcga
      721 aatggcttcg attacttaga taataaagtc tgtattgtca aaaattcaat agattattcc
      781 ggattagttt gtgccactgt tgatgaagac ttagagtatc aaatttttaa cacagagctg
      841 tttgaagaat acacgtacga agtcaccgga aatatatacg agaatagcga gttattggag
      901 gaacagcgat gaataaacag gaagcaatca aaaagttaga aagtattaaa gcgataggaa
      961 atgatgcaat agctgcttgc tataacgaga gtataaattc aggtattacg ttaatgaaaa
     1021 aaatagacga accgcagaaa ccagttattc cacaactcgt ggccggttgg cttgagaaat
     1081 ctacggaccc ttttacaaaa gctgaaaaaa tagcgtattt aatcaaatct aaagatggtg
     1141 attcatatta tttctgtgat tggtttgtac gagatggcat agtgacgcaa gagcaaggag
     1201 aagaattact cgcttgggca acaagacaat catatgaaac actattgagc ctatacaacg
     1261 gctacgaggt tgagaaagag ccgttatggg caataaagaa tgccgatgga aactatctta
     1321 ctaaatgtgc tttatgggga aaagatggag taaattatag ttttgaatgc aatccatctc
     1381 atcgattgct tttcactgat aaagcaacag cggatgctgc agcattgttg gtgaatggaa
     1441 cagtggaaga ggtggtaga
//
LOCUS       DAFEPQ010000171         1456 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.182, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000171
VERSION     DAFEPQ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.182"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            108..1040
                     /locus_tag="LP543_002821"
     CDS             108..1040
                     /locus_tag="LP543_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBL2322910.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1456
                     /locus_tag="LP543_002822"
     CDS             1112..>1456
                     /locus_tag="LP543_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322911.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          227 g          420 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagat
//
LOCUS       DAFEPQ010000172         1449 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.183, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000172
VERSION     DAFEPQ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.183"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="LP543_002823"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="LP543_002823"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBL2322912.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
     gene            complement(1103..>1449)
                     /locus_tag="LP543_002824"
     CDS             complement(1103..>1449)
                     /locus_tag="LP543_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL2322913.1"
                     /translation="NQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPGT
                     PSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRKSLDYRTPLEVFL
                     SYVSIDDLSNLI"
BASE COUNT          504 a          198 c          241 g          506 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta acttcttaat tgtcaaatta agttagacag atcatcaata cttacgtaac
     1141 tcaaaaatac ttccaaaggt gttcgataat ccagtgattt tctaggaata ttatttcgtt
     1201 tagatgcgac agattgaata aaaaattcat ctactgaatt gaaatccata gatttcaata
     1261 aaccatctct acgtaacaat ccgttagaat tctcgtttag gcctctttga gacggcgttc
     1321 ctggatcagc gaaataaatg gcaatatcat tgacattact gatctgtttc caatttgaaa
     1381 attcctttcc acaatcaaaa gtgatggatt tgaataggtt tttcggtaca gattcaaacc
     1441 attgattta
//
LOCUS       DAFEPQ010000173         1424 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.184, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000173
VERSION     DAFEPQ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.184"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(150..1337)
                     /locus_tag="LP543_002825"
     CDS             complement(150..1337)
                     /locus_tag="LP543_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBL2322914.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DAFEPQ010000174         1409 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.185, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000174
VERSION     DAFEPQ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.185"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..1359
                     /locus_tag="LP543_002826"
     CDS             <1..1359
                     /locus_tag="LP543_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBL2322915.1"
                     /translation="LFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKE
                     HLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSS
                     FKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNW
                     HIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPI
                     VYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPK
                     GKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDP
                     VAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEG
                     ARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFS
                     RN"
BASE COUNT          466 a          255 c          299 g          389 t
ORIGIN      
        1 ctttttagtg atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca
       61 gtaaaaggac atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata
      121 acagagaaag aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag
      181 atgaaagata tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg
      241 gattgtctca cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt
      301 tcttctttta aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat
      361 agtttagtgg cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa
      421 gagaactatt ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg
      481 catattaaag cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt
      541 aatcaagaag agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa
      601 acagatacct attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat
      661 tatcaccacg acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca
      721 gggtatttac actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc
      781 tattgtttcg cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac
      841 acggatattc ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag
      901 aaatggaaag acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca
      961 ttatttattg ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg
     1021 gatgcagccg ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat
     1081 ggtcgtttag aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga
     1141 cggaaaaatt ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg
     1201 agtgtatata aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta
     1261 tttgataaga taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct
     1321 tggaacaagg atgtacaaca acatttttct cgtaactaaa aacgccaaac attcccgatt
     1381 ctattctata ggtcgggtta tttgacgta
//
LOCUS       DAFEPQ010000175         1381 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.186, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000175
VERSION     DAFEPQ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.186"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..1307
                     /locus_tag="LP543_002827"
                     /note="aph(2'')-Ia"
     CDS             <1..1307
                     /locus_tag="LP543_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HBL2322916.1"
                     /translation="LKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYP
                     KTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRA
                     YQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSI
                     EIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYS
                     YISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECT
                     IDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNH
                     LLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKA
                     KEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          593 a          103 c          232 g          453 t
ORIGIN      
        1 cattaaaaaa acattataca gagccttggg aagatgaagt ttttagagta attattgaat
       61 ataacaatgt tcctattgga tatggacaaa tatataaaat gtatgatgag ttatatactg
      121 attatcatta tccaaaaact gatgagatag tctatggtat ggatcaattt ataggagagc
      181 caaattattg gagtaaagga attggtacaa gatatattaa attgattttt gaatttttga
      241 aaaaagaaag aaatgctaat gcagttattt tagaccctca taaaaataat ccaagagcaa
      301 taagggcata ccaaaaatct ggttttagaa ttattgaaga tttgccagaa catgaattac
      361 acgagggcaa aaaagaagat tgttatttaa tggaatatag atatgatgat aatgccacaa
      421 atgttaaggc aatgaaatat ttaattgagc attactttga taatttcaaa gtagatagta
      481 ttgaaataat cggtagtggt tatgatagtg tggcatattt agttaataat gaatacattt
      541 ttaaaacaaa atttagtact aataagaaaa aaggttatgc aaaagaaaaa gcaatatata
      601 attttttaaa tacaaattta gaaactaatg taaaaattcc taatattgaa tattcgtata
      661 ttagtgatga attatctata ctaggttata aagaaattaa aggaactttt ttaacaccag
      721 aaatttattc tactatgtca gaagaagaac aaaatttgtt aaaacgagat attgccagtt
      781 ttttaagaca aatgcacggt ttagattata cagatattag tgaatgtact attgataata
      841 aacaaaatgt attagaagag tatatattgt tgcgtgaaac tatttataat gatttaactg
      901 atatagaaaa agattatata gaaagtttta tggaaagact aaatgcaaca acagtttttg
      961 agggtaaaaa gtgtttatgc cataatgatt ttagttgtaa tcatctattg ttagatggca
     1021 ataatagatt aactggaata attgattttg gagattctgg aattatagat gaatattgtg
     1081 attttatata cttacttgaa gatagtgaag aagaaatagg aacaaatttt ggagaagata
     1141 tattaagaat gtatggaaat atagatattg agaaagcaaa agaatatcaa gatatagttg
     1201 aagaatatta tcctattgaa actattgttt atggaattaa aaatattaaa caggaattta
     1261 tcgaaaatgg tagaaaagaa atttataaaa ggacttataa agattgatta tataatatat
     1321 gaaaagctat tataaaagac attagtatta aatagtttaa aaaaatgaaa aataataaag
     1381 g
//
LOCUS       DAFEPQ010000176         1311 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.187, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000176
VERSION     DAFEPQ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.187"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            200..742
                     /locus_tag="LP543_002828"
     CDS             200..742
                     /locus_tag="LP543_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322917.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1248
                     /locus_tag="LP543_002829"
                     /pseudo
     CDS             898..1248
                     /locus_tag="LP543_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414636.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          532 a          148 c          254 g          377 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc t
//
LOCUS       DAFEPQ010000177         1281 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.188, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000177
VERSION     DAFEPQ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.188"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(<1..>1281)
                     /locus_tag="LP543_002830"
     CDS             complement(<1..>1281)
                     /locus_tag="LP543_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL2322918.1"
                     /translation="IYTKNLTGLDLEIDAQSVLVGVYPFISSSSEGEDEITLPEEVIF
                     TDYVDDYPAGYVSFVDFKDKATDVATLREAAKDWLKTNIDKQKPQVSGSIELVPLRHQ
                     RGYEKFVDLEKVSMGDGVDVYHPQLKVNMSARIVEYTFNVLTNSYDKLVVGNVKTNFL
                     ENTENNVSNLINDAIDQLKNGGEISDLLNDIVDHQTDMITGQNGGYVLLDPKEAPSRI
                     LIMDTPDKNTARNVLQINNAGIGFSKTGINGTYETAWTLDGGFNASFITAGEIVGITI
                     RGTTLISDGADYRTSIANGKMTWYSKKVNKDIMELEARDYVSADAGIVSYTMKTGGGF
                     MIRNPQGNLVFSTWDNGNNRPFLSFGAPNFRYSNASYVTSGDGSSLSINGSAGNSWEF
                     KVAGRTMKFTSDGMLTLPGCFFGSWEDGKLARFEQ"
BASE COUNT          395 a          289 c          176 g          421 t
ORIGIN      
        1 ttgttcaaac ctagcaagtt tcccatcttc ccatgaacca aaaaaacaac ctggtaacgt
       61 tagcatacca tcactagtaa atttcatagt cctaccagct accttaaatt cccatgagtt
      121 acccgcacta ccattaatgc ttaaagaact accgtcgcca gaagttacat aactagcatt
      181 gctatacctg aaattgggcg caccaaaaga tagaaacggt ctgttattac cattatccca
      241 cgtactaaaa accaagttac cctgtggatt tctaatcatg aaaccaccac cagttttcat
      301 ggtgtatgat acaataccgg catcagcact tacataatca cgtgcttcta gctccataat
      361 atctttgtta actttttttg agtaccaagt cattttgcca ttagcaatac ttgttctata
      421 atcagcacca tcactaatta atgtagtacc tctaatagta attcctacta tttcaccagc
      481 cgtaataaac gaggcattga atccgccatc taacgtccat gccgtttcat atgttccatt
      541 aatgccagtt ttagaaaaac caataccagc attgttgatt tgtaaaacat tccttgcggt
      601 attcttatct ggtgtgtcca taatcaaaat acgactaggc gcttctttag gatctaataa
      661 aacataacca ccattttgac cagtaatcat atcagtttga tgatctacaa tatcattgag
      721 taaatcactg atttcgccac cgtttttcaa ttgatcaatg gcatcattaa tcaaattgct
      781 gacattattc tctgtgtttt ctaagaagtt tgttttgacg tttcctacaa ctaatttatc
      841 gtatgaattg gttagaacat taaacgtata ttccacaatt ctcgctgaca tattcacttt
      901 taactgtgga tgatacacat ctactccgtc acccatcgaa actttttcta gatcaacaaa
      961 tttttcatag cctctttgat gcctcaatgg tactaattca atcgaaccac tcacttgtgg
     1021 tttttgttta tctatgtttg ttttcaacca gtctttagca gcttccctta atgtggctac
     1081 atcagtcgct ttgtctttaa aatcaacaaa agaaacatat ccagcaggat aatcatccac
     1141 gtaatccgtg aaaataactt cttctggtag agtgatctcg tcttctcctt ctgaagagct
     1201 gctaatgaat ggataaactc caactaaaac actttgagca tcaatctcta agtcaagacc
     1261 agttaagttt ttagtataaa t
//
LOCUS       DAFEPQ010000178         1277 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.189, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000178
VERSION     DAFEPQ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.189"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            21..>1277
                     /locus_tag="LP543_002831"
     CDS             21..>1277
                     /locus_tag="LP543_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBL2322919.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRI"
BASE COUNT          427 a          250 c          250 g          350 t
ORIGIN      
        1 ctgagaggac gatacgctat atgaatgatt ctatcaaaaa aatgctgaga ataacagaga
       61 aagatttgat gattacagag gtctcttacg agacccttca gaagaaaaag acgttggtcg
      121 tcgatgctgt tctctcgcct actcctcgtg cttgtagaag ttgtggttct actgtggtag
      181 atggaaacgg gaaagcaatt atagtgaaaa atggaaaaaa ggaaacgatt gtccgttttg
      241 aacaatacaa tcatatgcct ttggttatgc gcctaaaaaa gcaacgctat acctgtaaaa
      301 actgtcgcac ccattggacg gctcaaagtt attttgtcca acccagacat tcaatcgcaa
      361 atcatgttag atataaaatt gcttctttac tgactgaaaa agtatcttta tcttttattg
      421 cgaaaagctg tcaggtatct ttgaccaccg ttattcgtac attgaaagag tttaaaagct
      481 atttaccaaa gcaatctaag aagatcctcc ccagagtatt gatggttgac gaatttcgtt
      541 cgcatgcttc catagaagat aagatgagct ttatttgcgc ggatggcgaa acaggaaaat
      601 taatagatgt tttgcctacg cgtaaattac ctcgattaac aagctatttc ttaggttgta
      661 ccaatccaga agaagtagaa ttcttggtga cagacatgaa cgccgcctac ttccagctca
      721 ccaaacgtgt tctgccaaat gcgaaagtcg tgattgatcg gtttcatatt gtcaaacaca
      781 tgaatcaagc gttcaatgag ttgcgtatcc gtgaaatgaa tgaacttcgt aaagccggac
      841 agaaaagcca ggcagaaaaa ctgaaaaaga actggcgctt tttgctaaaa aatcgtgcaa
      901 acatcaacca ttatgaatac aaaacatgga aaagtttccg agcaccaaaa tacccatttc
      961 ttactgaagc aatgatgatt gatcgattgc ttgagttttc tccgccccta aaggaggcgt
     1021 atcccttttt tcatgaatta gttgaagcct ttcgagacaa agaccctgac ttatttttct
     1081 ccttattggc agaacttccc gaaacgttag atgacagctt tcgggaaaag cttcaaaacc
     1141 ttctgaccta tgaagaaggc atcaccaacg caatgatcta tccttattcc aatggaaaaa
     1201 tagaagcgaa gaatacccac ataaagacaa tgaaacgagt atcctacgga tttaaatcat
     1261 ttgagaacat gagaatt
//
LOCUS       DAFEPQ010000179         1276 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.190, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000179
VERSION     DAFEPQ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.190"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            162..>1276
                     /locus_tag="LP543_002832"
     CDS             162..>1276
                     /locus_tag="LP543_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HBL2322920.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFT
                     KEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVK
                     GEREKQFAYSAHTSCDDNGFILSTIITPGNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKEKKGLLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCTKSKNHQRVITR"
BASE COUNT          413 a          244 c          252 g          367 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagtcagact gtagacaaaa gctgtctgtg
       61 aggacagctt cagactgtag acaaagtcca ttttatggat gatgtctaca gtcttttctt
      121 ttataataga tttatgaaaa cgaacgcaag gagtgatagc tatgatgtct aaaaacaata
      181 cgaatggtag aaatcaattt gcgatgctta cgattgatga tctagttcct caagatcatc
      241 tcgttcgaaa gattgatgcc gcgcttgatt ttgaatttat ttatccaatc gtcgaagcca
      301 cttactccga tttaggtcgc ccaagtatcg atccagtgat tctgatcaaa ttggtgttta
      361 ttcagtattt attcggtatc cgttccatgc gacaaaccat taaggaagtc gataccaatg
      421 tcgcttaccg ctggttttta ggctactcct tcgaggagaa aatcccccat ttctcaactt
      481 ttggaaagaa ttatgttcgc cgatttagag aaacgacggt gtttgaggat atttttgcct
      541 atattttaga acaagcagtt aaagcaggtt ttgtaacaga ggataatttg tatctggatt
      601 ctacccacat caaagctaac gccaataaac ataagtttac gaaagaaatg acccatgatg
      661 aagcgaaagc ctatcaggat gaattagaag atgagatcaa tcgtcaacga atagaggctg
      721 gaaaacgccc ttttactttg gacttagaaa aggaagtgaa gctgaaagaa agaaagataa
      781 gtaaagctga tccggaaagc ggctactacg tcaaaggcga acgagaaaaa caatttgctt
      841 attctgccca tacttcatgc gacgacaatg gatttattct tagcacaatc atcactcctg
      901 gaaatattca tgacagtcaa gtcgcctttc agctcgtcaa acaatcgaag agactttttc
      961 ctgaaatcaa ttgtgttgtc gctgatgctg ggtataagac acctaagttt gttcactttt
     1021 tgactcattt gaaccttcga ccttgtttgc cttattcaag gcccaaagag aaaaaggggc
     1081 tattatcaaa aaatgagttt ctctatgacg agtattttga ttgttatatt tgtccgcaag
     1141 atcaaatgct cgctttttca acggttactc gagaagggta ccgagaatac aaatccaatc
     1201 caaaagaatg tgtgaactgt cctctgctaa accaatgtac caaaagtaaa aatcatcaac
     1261 gagtcatcac gagaca
//
LOCUS       DAFEPQ010000180         1251 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.191, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000180
VERSION     DAFEPQ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.191"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            102..248
                     /locus_tag="LP543_002833"
     CDS             102..248
                     /locus_tag="LP543_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322921.1"
                     /translation="MILNNPEYIESGKAETVNYYYELPSEIQNYQIVIREADGYFVGL
                     PFTL"
     gene            385..699
                     /locus_tag="LP543_002834"
     CDS             385..699
                     /locus_tag="LP543_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322922.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFRLFRDQIIGISQT
                     TK"
     gene            798..1046
                     /locus_tag="LP543_002835"
     CDS             798..1046
                     /locus_tag="LP543_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL2322923.1"
                     /translation="MDTITPTNFKKNIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDLMKNEKEDDFKVV"
BASE COUNT          485 a          136 c          241 g          389 t
ORIGIN      
        1 aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt tccaccaaca caaaatatta
       61 tagaaccaaa taaaaatggt gaagaacttt atgagtatgc tatgatattg aataatccag
      121 aatatattga aagtggcaaa gcagaaactg ttaattatta ttatgaactt ccgagcgaaa
      181 tccagaatta tcaaatcgta atacgtgaag cagatggtta ttttgttggc ttgccattta
      241 ctttgtaatc ttttatttga aagtggctta tactttaatt gaattagatg taggactgtc
      301 aagataatat cacgagtgat tagagacaaa gaatctatat caattttacg attttttgaa
      361 aatgaaagtg aggaaattgg aagaatggct gatgaaaata aagttgttcc gcttgaaaca
      421 ctagtgataa ataaggaaga gtttgagcaa ctttttcttg atttttttgt tcaatatgac
      481 aggatttatg attttagttc gtcactagat ttatttgaat tagaagcaga aataatagaa
      541 aacagtcgag aggagaatgg taaaatcatc atttcagtag aaaaattcaa tgaattgttc
      601 tcagaaacat ttgaaataga tacagattat tatcaaccac aagaagtttt tcgattgttt
      661 agagatcaga ttattggtat tagtcaaact acaaaatgaa agcttaataa agcaagcgtt
      721 gaaagaatta ttcctcaacg cttgctttat ttgatgggcg cttcataata gaggtataga
      781 gaagagaggt gttcattttg gatactatta ctccaacaaa cttcaaaaaa aatataggta
      841 aagtgttgca agaagtagtt cattcaaatt ctgaaataga aattactagg gattctaaaa
      901 atggattgaa cgatggcgtc attatgattt ctaagagaaa gtgggagctg ctacaagaag
      961 aactttattt acaacgaacg ggtacatggg cttatgtatc tgatttaatg aaaaatgaga
     1021 aggaagatga ctttaaagta gtttagtatg aaggtcgatt gataaaacga tgtggattaa
     1081 tgctaaataa tgagtaattt ttcaataata caaaaactgt gtacaatatt ataagtagag
     1141 aaacgctagt gtcgaaaaaa gatatttttc gtgaaaagta ataggggcta aaaagaaagt
     1201 atacaattta tttatgtttc taagcatata aaaaagagcg ttgaaaggaa t
//
LOCUS       DAFEPQ010000181         1195 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.192, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000181
VERSION     DAFEPQ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.192"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..1076
                     /locus_tag="LP543_002836"
     CDS             <1..1076
                     /locus_tag="LP543_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL2322924.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAFEPQ010000182         1166 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.193, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000182
VERSION     DAFEPQ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.193"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..885
                     /locus_tag="LP543_002837"
     CDS             <1..885
                     /locus_tag="LP543_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL2322925.1"
                     /translation="TNDSLETTIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSK
                     LVAEDVLAFKERTLEANYSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFT
                     IAPTESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSR
                     NIAHKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALL
                     TFYNFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFL
                     GRIHKGFKEIQDTLESMI"
BASE COUNT          404 a          194 c          191 g          377 t
ORIGIN      
        1 accaatgatt cattagaaac gaccattatc caattatttc aaaaaggcat tactatggct
       61 gaaatctcca aattaattga aaaaatgtat ggtcaccact atacgccaca aacaatctcc
      121 aacatgagta aattggtggc tgaagatgtt ttagctttta aagaaagaac gttagaagcc
      181 aattattccg ttatatttat ggatgcgacg catatccctg tgaagcgaca gactgtttcg
      241 aaggaagcag tgtatattac gattggtatt cgtttggatg gaaccaaaga agttcttggg
      301 tttactattg ccccaactga gtctgcttat atttggaaag aagttcttca agatcttaga
      361 aaacgtgggt tagaagaagt tttattagta gtgacagacg gattaagctg tattgaagaa
      421 agtatccata gtgtgtatcc gaatgcccaa tttcaacaat gttgtgtgca tgtatctaga
      481 aatatcgctc ataaagttcg tgttcgaggt cgaaaagaaa tttgtgagga tttcaaattg
      541 gtttaccaag cgaattcaaa agaagaggca ttggatcaca tcgactttat gactaggaaa
      601 tggaaaaagc agtatccaag agtcgtcaat ttactcttga atcctgccct attaaccttt
      661 tataatttcc ctcacgccat cagacgaaca atttattcga cgaacctgat tgaaggcttt
      721 aataagcagc taaaacgata tactcgaaga aaagaacaat tccctaatga agaatctcta
      781 gagagattct tcatttctca atttaatcaa tataaccaaa aatttttagg tagaattcac
      841 aagggattta aagaaattca ggatacatta gagtcgatga tttaactgta attaacagaa
      901 tggattttcc atttacatat aattcttgac gctaccataa tctaaataac catcatattc
      961 atctactact tatataattg agttttatta aactttttaa gtttctattc tatcaaatat
     1021 ttaaacaatc tctgcatccg aaaagactac ctcgtatagt tttactaaca atatttttat
     1081 tagcgtgttc aaatcctgat ttcttgtatc aattattttc tgttcacttt caataagaaa
     1141 aaagtcttta cttccatgga taataa
//
LOCUS       DAFEPQ010000183         1161 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.194, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000183
VERSION     DAFEPQ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.194"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(275..1156)
                     /gene="galU"
                     /locus_tag="LP543_002838"
     CDS             complement(275..1156)
                     /gene="galU"
                     /locus_tag="LP543_002838"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBL2322926.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          294 a          260 c          181 g          426 t
ORIGIN      
        1 ttaaaaaatt cgccttttta ttctactatg aatcttctgg cttggctttc atttctctta
       61 tgtaatcatt ttcttttata caacttaaaa aaaaattaaa ccattagcat aatattttct
      121 gaatctattc acgaaagtct ttttctatct ttcgttttcc acgactcaac tgatctagag
      181 tcaaaaaact aaaacgacag ttgcgcaaat gacgcacaag ctgccgcttt aataacaaat
      241 agcctttcat tctcagcagg ccacttattt ccagttaact ttctgttctt agctttttac
      301 tcaactggat aatatatgca cttaatgaag tcccaatttc cgaatgttga agtccatatt
      361 caatatttgt cttcatatat cctaacttat cgcctacgtc gtatcttttt cctttaaact
      421 ctctcgcata gaccgcctct gtctggttaa gagtctgaag tgcatccgtt agctgtatct
      481 cgtttcctgc tcctggttct tgagtttcta atatctcaaa tattttagga gtcagtagat
      541 aacgtccgat aatggctaaa ttacttggtg cttttcccat ttcaggtttt tctacaaacc
      601 cttttactct gcaacgaccc ttttcgctat ctgttttagg atcaataatt ccgtatttag
      661 atgtttcatt ttctggtaca cgcatgacgg caacagcgga agcttgtgtt tcttcataat
      721 ccatcatcaa ttgttgagtc aatgggatat catctttcat caaatcatcc cccagcatga
      781 cgacaaacgg ttcatttcct ataaatgctt ttgcttgaag cacggcatcc cccagtcctc
      841 ttggatgaga ttgacggatg aagtgaagat tgacttgagt cgtttcttca actaaagcga
      901 gtaattctgt tttttccttt tcccgcaaat tgttttccaa ttcgatattt gcatcaaagt
      961 gatcttcaat gggtcttttt tcttttcccg ttacgatcaa gatgtcttcg attcccgaat
     1021 ccaatgcttc ttccacaata aactggatcg ttggtttatc cacgattgga agcatttctt
     1081 ttgccatggc ttttgttgcc ggcaaaaaac gtgttcctag cccagcggca ggaatgactg
     1141 cttttttgac cttcacttgc t
//
LOCUS       DAFEPQ010000184         1076 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.195, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000184
VERSION     DAFEPQ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.195"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            312..608
                     /locus_tag="LP543_002839"
     CDS             312..608
                     /locus_tag="LP543_002839"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL2322927.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLSTVCMKKLDVC"
     gene            complement(531..1076)
                     /locus_tag="LP543_002840"
                     /pseudo
     CDS             complement(531..1076)
                     /locus_tag="LP543_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
BASE COUNT          330 a          216 c          179 g          351 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttccacggt ctgtatgaaa aagcttgacg
      601 tctgttaaag aatacggtat ccgtccaaag gcttctttta ccaatgaggc atctttcttc
      661 tcaccacagg aataaccaat aatttctcga ttaaacaaat caagtattaa gcagatataa
      721 tgccactttt tccccacgcg aacataagta agatccgtaa cgatggcttc caatggctgt
      781 tcttgtgtaa aggtccgatc taatacattc gctgttttcg cttcattaca agctgttcgt
      841 tgccctttaa aatgagcgat cgtataggta gatgtcaatc cgcggcgttt catgattcga
      901 ccgattcggc gccgactgag ttgaagccca cgctttgcta aacacttctt caattttcgg
      961 gtaccgtaag cctttcggtt tcgaataaac tcttcctgaa cgatctcttc taagtcggac
     1021 tcattttcga tcggtttcgc ttgataatag taggtttgac gagaaatatt tagaat
//
LOCUS       DAFEPQ010000185         1057 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.196, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000185
VERSION     DAFEPQ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.196"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            66..337
                     /locus_tag="LP543_002841"
                     /pseudo
     CDS             66..337
                     /locus_tag="LP543_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751573.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
     gene            876..1057
                     /locus_tag="LP543_002842"
                     /pseudo
     CDS             876..1057
                     /locus_tag="LP543_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748821.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          371 a          152 c          193 g          341 t
ORIGIN      
        1 tttttgttga tttaccaaca tgtttagaca ttttcctcta ggaaaaaatt aactagcacc
       61 acgggttaaa tctatatttt gatatacaaa ttacagcttc agatactaag ttgcataaac
      121 cttttccgga ccccatacaa aaggcaattg atgaattggg cataaaaaat aagaaacgat
      181 ttatattggt gattcattat atgacatgca aagtgcgaaa gcttgtggga tagcatttgg
      241 tttagctaaa tggggagcca aaaatataga aaattttcaa agtgtagata taattattga
      301 ttctcctcaa gaaatacttg atttgttaga aaaatagaca aagagcgtga gataaggata
      361 gggtctcacg ctctttgttt ttcgccattt aaagattgaa atttttgcca tttaaaaaga
      421 aaagtgacag ataggaataa aaacttatta aatgtagtta tgatctgtta tatttcattt
      481 ctaatactag gtcttccaca agcagtgttt cctgaaatgt tttctggaga ctggggtatt
      541 gtcagctatt ctatattaac attgattgct atggttttag gtacttcatt ttataatgct
      601 tttgtaagta agcatgttga ggaatatgaa gtattaatgt acttaaaaga gaaaaaaaca
      661 agtaattaat atgagaacac cacgcatagt ttttattttg agatattcta gaatgaaaca
      721 cattaagaac gaaagcaaag caaaagacgc cagaacttct actaccttct aagttttttc
      781 atatttgttt gttatgctat acatagatct gcataattag acttgcgctg cccgttattg
      841 aagtttcagg cagcgctctt tttgggggat cgataatgga agaagaaaaa atatttgaga
      901 aacgctggca gctagcatca agtgaacaaa gagcaagata caataatttg atcacctcct
      961 accccactgt tgactagaca tataaggaaa agaaatactt actttggctg tgccagttag
     1021 atatagatac ctttgaaaca tttgaagtga tcctaga
//
LOCUS       DAFEPQ010000186          972 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.197, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000186
VERSION     DAFEPQ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.197"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            159..>972
                     /locus_tag="LP543_002843"
     CDS             159..>972
                     /locus_tag="LP543_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBL2322928.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKH"
BASE COUNT          354 a          166 c          179 g          273 t
ORIGIN      
        1 gttgaaagcg ttgtcaataa tataaatgtt aaacttactt attttttatc gattttaatt
       61 aaccaaaata ttaataatga ttaaaacaaa taatttaata taacttttat taacaaatat
      121 gttaatatat aagaaaaacc ttttaagagg tgatatatat gcaactacaa ataaacgaag
      181 aatccttacc ggtatatgaa gcattagcta gtaaaacaag gatcaagatc atccagctac
      241 tttccaaaaa aaagatgaat gtcaaagatt tggcaaaaga acttggagtg agcagtgcta
      301 tcacgacgat gcacgtgaaa aagctagagg aagcaaacat catcaaaaca gaaaaagtag
      361 gacaacaaaa aatttcgagc cttcgtgtcg ataaaatcga tattagtttt ccagaaaaaa
      421 tattcaatgc ttttgatacg aaagaaacat cgatacctat tggtcactat acaaattatg
      481 caattgaacc aacatgcggt ttagctacga tccatgattt tatcgggaaa gtggatgaac
      541 cacgctattt tatggatcct cgaaggatgg atgcccgcat tctctggttt accagcggat
      601 ttgtcgagta tcaggcacct aattttttaa atacagagga tacattggag atgttggaag
      661 tttctgtgga aatttcttct gaatttcctt tttcaaatga caattggcct tcagatatca
      721 ctttcagcct aaatggtgtg gagctaggaa catggacgag ccctggagat tttgctgata
      781 tccgcggtaa atatacgcct gattggtatc cagataatct taatcaatac ggcttgctga
      841 agacgatccg aattaccaaa cacttgacga atatgaatgg agaacccttg tcaaacatca
      901 cgatcaacga catccctaaa gagcaagaca cttggcattt gcgaatcgaa gtgaaagacg
      961 atgccaaaca tg
//
LOCUS       DAFEPQ010000187          944 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.198, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000187
VERSION     DAFEPQ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.198"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(<1..>944)
                     /locus_tag="LP543_002844"
     rRNA            complement(<1..>944)
                     /locus_tag="LP543_002844"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          199 a          293 c          212 g          240 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtc
//
LOCUS       DAFEPQ010000188          909 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.199, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000188
VERSION     DAFEPQ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.199"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            440..664
                     /locus_tag="LP543_002845"
     CDS             440..664
                     /locus_tag="LP543_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322929.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMIREAT
                     PVEIDYCKLVYCGCGRWKEDHVQKNISKYI"
     gene            682..>909
                     /locus_tag="LP543_002846"
     CDS             682..>909
                     /locus_tag="LP543_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322930.1"
                     /translation="MEKSDTKANRYKPTAAEKKLLEVLINPDNLGKPVQELCSLAKIG
                     RTKYYDAMSKDGFVDLVNETTMDLIKGKASDV"
BASE COUNT          323 a          135 c          201 g          250 t
ORIGIN      
        1 aacatcgttt tattattgtt tcacaattaa gcttcgatag acagcaacgg aaatattaag
       61 aataaggatg tgaattttaa ctccttctaa attgttctta ttatctatca tccgttgctg
      121 tctattgtta catacagatc gcttaggcgg tctttttatt ttggccgaaa acctacattt
      181 tcgatggccg attattggag gaggaatagc atgttcaaac tatctgaaat cattaagaag
      241 tccgacgttg agaaattaga gatgttaaaa aagaagttga agaaatagca gtctcttcgt
      301 gaggctgttt tattttgctc acaaaaatag accactatcg ggtaatagtg gtcaggaatt
      361 aaatgaaaaa gatgttaggg ttgttagcta agtataacat cataacgctt acaaaacaat
      421 gcaagaagga taaattatta tgagaactta ctggtatgtg tcattaaaca ataaatatcc
      481 gaagccaatg aaaggacagc ataggcgtgt agtcatgtct gttcaaatga aggcgaagta
      541 ttcgattgta gaaatgatca gagaggcaac gccagtagag attgattatt gcaagctagt
      601 ctattgtggg tgcggccgtt ggaaagagga tcatgtacaa aaaaatatca gcaagtacat
      661 atagagaggc ggtgattaag gatggaaaaa agcgatacga aagcgaacag atacaaacct
      721 actgctgcag agaaaaaact cttggaagta ttgataaatc cagataactt aggcaaaccg
      781 gtgcaagagt tgtgttcact ggcgaaaata ggtaggacca aatattacga cgccatgagc
      841 aaagatggct ttgttgatct agttaacgaa acaacaatgg atctgatcaa aggaaaagct
      901 tctgatgtc
//
LOCUS       DAFEPQ010000189          890 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.200, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000189
VERSION     DAFEPQ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.200"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..888
                     /locus_tag="LP543_002847"
     CDS             <1..888
                     /locus_tag="LP543_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBL2322931.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DAFEPQ010000190          843 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.201, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000190
VERSION     DAFEPQ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.201"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            77..>843
                     /locus_tag="LP543_002848"
     CDS             77..>843
                     /locus_tag="LP543_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HBL2322932.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSR"
BASE COUNT          307 a          158 c          143 g          235 t
ORIGIN      
        1 aatccataca aaaaaggaag tcccttctgt agaataaagt taacgacaac caattcacag
       61 aaaagaggac ttccctatga atgattttac tacagaaatt gtgcaaactc tagtcactaa
      121 aggcgattta aatgaattat tccgttcgca cttagaaaaa gcgataaaca cactcctacg
      181 gactgaatta acggcttttt tagattacga aaaatatgat cgcactggtt ttaattcagg
      241 taattcgaga aacggttctt actttcgatc aatcaaaacc gaatatggtg aattaacatt
      301 ggaaatacct agagatcgta atggtgagtt taaacaacaa actttaccag cctacaaaag
      361 aacaaacgat acattggaaa ccactattat ccatttattc gaaaaaggtg ttacgatgtc
      421 tgaaattgct gatttgatcg aaaaaatgta cggtcatcac tatactccac aaaccatgtc
      481 caacatgact aaagttctga ctgaagaagt aaatgccttt aaatccagag ccttaaatga
      541 taagtatgtc gctattttta tggacgctac ttacattcca ctaaaacgtc aaaccgtatc
      601 caaagaagcg atttatattg ccattggtat acgagaagac ggcactaaag aagtactgag
      661 ttatgcgatt gctccaactg aatcaacata cgtttggaat gagctgctac aggatattaa
      721 ctccagagga gttcaagaag tcttgctttt tattacggac ggcttaaaag gcatgaaaga
      781 tactatccat caaatttatc ctaaagcaaa atatcagcat tgttgtatcc atgtatctcg
      841 taa
//
LOCUS       DAFEPQ010000191          841 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.202, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000191
VERSION     DAFEPQ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.202"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(<1..146)
                     /locus_tag="LP543_002849"
     CDS             complement(<1..146)
                     /locus_tag="LP543_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322933.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGR"
     gene            complement(248..601)
                     /gene="tnpB"
                     /locus_tag="LP543_002850"
     CDS             complement(248..601)
                     /gene="tnpB"
                     /locus_tag="LP543_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBL2322934.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(591..785)
                     /locus_tag="LP543_002851"
     CDS             complement(591..785)
                     /locus_tag="LP543_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322935.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          244 a          168 c          146 g          283 t
ORIGIN      
        1 cgtccaaata atttgttggt taaataattg accttttcat tcaatagttg gatttcagtt
       61 gtaagattct gaatggtttg ttgttgtgct tgattggttt tatgggcttc ttgaagcaac
      121 tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa ctgattttgt
      181 tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt tgtgttaacc
      241 ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga tcgatactta
      301 atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg tctttcgtcc
      361 gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa ccatcaccgt
      421 cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa acggcatctt
      481 cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca attccttttc
      541 gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa tcataaagca
      601 ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc gttatagatc
      661 aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc cgcgggttta
      721 cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc atttaattta
      781 tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag
      841 t
//
LOCUS       DAFEPQ010000192          819 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.203, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000192
VERSION     DAFEPQ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.203"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(<1..557)
                     /locus_tag="LP543_002852"
     CDS             complement(<1..557)
                     /locus_tag="LP543_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322936.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLG"
     gene            complement(581..>819)
                     /locus_tag="LP543_002853"
     CDS             complement(581..>819)
                     /locus_tag="LP543_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322937.1"
                     /translation="DPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNV
                     LVRWNLVAHRLGYEDIRLKEDTAYDSTVLDQKRF"
BASE COUNT          234 a          169 c          127 g          289 t
ORIGIN      
        1 ttacctaact tagaaatttc ttctttcaga gcatctaact tgtcttgtag cgttttagct
       61 tgaccagtta aatcagtaat ctgttgattt aagctatcca ctctaccttt gatttcagcc
      121 ataaaagcat caaaagtttc gttgtacttt cgaatcaact cttctaattg cgaaacatat
      181 tcatcggctt ggccttgcga aatgtcagac actcctagtg agaaaaaaat gatatcttgc
      241 gttgttaaaa tttgattgtc ttttctatat tctacgtagc agtgtttata atatcctgct
      301 tcactcataa atgtgccatc aagagaaaac gtgacttctt cactagttac actagttgca
      361 acattatcta cgtaacggtt agatggtgtt gttcctttta aagtaaatgt tccgccactc
      421 gtatccatct gcaagccatt tagaaacggt ttaaccgtca ccgtaatccc tttatcacct
      481 tgacgagcca taatagcttt ggtgtagttt aattctttgc tgaaatctaa agccaaatta
      541 tataaactgc tagccattta tatacctcct tgtcttcgtt ttaaaaacgt ttttggtcaa
      601 gcactgtgct atcatatgct gtatcctctt ttaatctaat atcttcatac cctagacggt
      661 gtgccactaa attccatcta actaatacgt ttggcttact agtttcaatg atgaaatggt
      721 caatatcttc atgagtaaca gcacacaaaa ctagttctgt aggtgtcaca tgtgtcatat
      781 accgacttag atttactgtt tcagcaaaca tggggtcaa
//
LOCUS       DAFEPQ010000193          804 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.204, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000193
VERSION     DAFEPQ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.204"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(248..727)
                     /locus_tag="LP543_002854"
     CDS             complement(248..727)
                     /locus_tag="LP543_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322938.1"
                     /translation="MCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNSL
                     DFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKCFESAVVKFPQFKLNP
                     AASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKKAFKIKTIGTAPNFK"
BASE COUNT          269 a          112 c           92 g          331 t
ORIGIN      
        1 gacgctccct taacaataaa tgaagacttt tttattgtta attgtttatt tttctattat
       61 tagatatgaa aatcgcattt agataaaata gaaaaatatg aatatatttt aaatattaag
      121 atgagagtca ttaacttatg atagtagtca atttgctttt cactaattac aaaatatgtg
      181 atataggaag tttagttatt gttctcataa tgccatcctg tttttagatt atctagtaat
      241 ttttttgtta tttaaaatta ggagcagtgc caattgtttt aattttaaag gcttttttta
      301 tatcttctaa attagaagat atttgtatat caatatggaa tatattacct tttttgtatt
      361 tttttatgtt atctttagct aaaacatcaa atacatgtcc ttgaaattgt atcgaggcag
      421 ctggatttag tttaaattga ggaaacttta ccacagctga ttcaaaacac ttgtcacatt
      481 tttttatttt tttacctttg taaaaatatt cgattgaata ttctatttgg cacgactcac
      541 ttggcagtat atgtatgtca atatttttca tctgtttcat aggtatttca aaatctaaac
      601 tattaaaaac atataattcc ctgtaattat ttacatctaa ctctactaaa tttatctttg
      661 gaacaataga tcctccaccc attccaccat aataaaaaaa atcttgttct ctatcaaaat
      721 agcacatatt aagctcagtt tgtttatcat cctcaataat tttataaatc aaacctaccc
      781 gtggtgctag ttaattttaa tatc
//
LOCUS       DAFEPQ010000194          790 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.205, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000194
VERSION     DAFEPQ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.205"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..781
                     /locus_tag="LP543_002855"
     CDS             <1..781
                     /locus_tag="LP543_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL2322939.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          339 a          107 c          102 g          242 t
ORIGIN      
        1 agatatgcta gattactact attttgaaaa aaatttatca tataattatg taatacattt
       61 aaaaactttt acaggaatga tttttaaatt cgccaaccgt cgatatggat taaaatataa
      121 tcccgtaaat ggggtttctt tagcaagaaa acctaagaca aacgaagaaa taaaaaaaga
      181 aaaagatggt tatttatcaa aagaagaagt tcaaatgatt gctagtaaac aaaaaagttc
      241 acatcaacag tcacgttatt ctttaattac taaatttttg tttttaactg gactacgtta
      301 tggtgaattg atatctttga ctgaatctga ttatgatggt aataaaatta cggtttctgg
      361 tacttatgat tacgagttaa aaattaaaac aacaactaaa aatacaggtt cttatagaac
      421 cattgaatta tcaagtaatg ctaaagaaat catagatcaa ttaattgaag aaaacaaatt
      481 aattaagaat aataaagata aatacatttt catctctaaa aatggtaacc cgatatctat
      541 tcaagcatat aatcaatctt taagagcagt atccaaagaa ttaaacatag ataaaaaagt
      601 ttcttctcac atgttaaggc attcccacat ttctctactt acagaattag gaatcccatt
      661 aaaagccatt atggatcgtg taggacatga agattcaaaa acaacattaa agatttatac
      721 tcatacaact aaaaatatgc aaaatcaact agtagaaaaa ctaggaaaaa tagaaattta
      781 aattgcccct
//
LOCUS       DAFEPQ010000195          787 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.206, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000195
VERSION     DAFEPQ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.206"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            24..716
                     /locus_tag="LP543_002856"
     CDS             24..716
                     /locus_tag="LP543_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBL2322940.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          195 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttat
//
LOCUS       DAFEPQ010000196          775 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.207, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000196
VERSION     DAFEPQ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.207"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..>775
                     /locus_tag="LP543_002857"
     rRNA            <1..>775
                     /locus_tag="LP543_002857"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          237 a          156 c          228 g          154 t
ORIGIN      
        1 ctttcagata gtcgaatgac ttggaaaagt cagtcaaaga gggtaaaaac cccgtagacg
       61 aaatgtggaa gacacctagg aggatcctga gtacggcgga acacgagaaa ttccgtcgga
      121 atccgggagg accatctccc aaggctaaat actccctagt gaccgatagt gaaccagtac
      181 cgtgagggaa aggtgaaaag caccccggaa ggggagtgaa atagaacctg aaaccgtgtg
      241 cctacaacaa gtcaaagccc gttaatgggt gatggcgtgc cttttgtaga atgaaccggc
      301 gagttacgat tgcatgcgag gttaagttga agagacggag ccgcagcgaa agcgagtctg
      361 aatagggcgt ttgagtatgt agtcgtagac ccgaaaccat gtgatctacc catgtccagg
      421 ttgaaggtgc ggtaaaacgc actggaggac cgaacccacg tacgttgaaa agtgcgggga
      481 tgaggtgtgg gtagcggaga aattccaaac gaacttggag atagctggtt ctctccgaaa
      541 tagctttagg gctagcctcg gaattgagaa tgatggaggt agagcactgt ttggactagg
      601 ggcccatctc gggttaccga attcagataa actccgaatg ccattcattc atatccggga
      661 gtcagactgt gagtgataag atccatagtc gaaagggaaa cagcccagac caccagctaa
      721 ggtcccaaaa tatatgttaa gtggaaaagg atgtggggtt gcacagacaa ctagg
//
LOCUS       DAFEPQ010000197          767 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.208, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000197
VERSION     DAFEPQ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.208"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..767
                     /locus_tag="LP543_002858"
     rRNA            <1..767
                     /locus_tag="LP543_002858"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          189 a          169 c          243 g          166 t
ORIGIN      
        1 cgtagaaatc ggaacgctag tttcgatgga ggcgctggtg ggatactacc cctgcgttat
       61 ggccactcta acccgcacca ctaatcgtgg tgggagacag tgtcagatgg gcagtttgac
      121 tggggcggtc gcctcctaaa aggtaacgga ggcgcccaaa ggttccctca gaatggttgg
      181 aaatcattcg aagagtgtaa aggcagaagg gagcttgact gcgagaccaa caagtcgagc
      241 agggacgaaa gtcgggctta gtgatccggt ggttccgcat ggaagggcca tcgctcaacg
      301 gataaaagct accctgggga taacaggctt atctccccca agagtccaca tcgacgggga
      361 ggtttggcac ctcgatgtcg gctcgtcgca tcctggggct gtagtcggtc ccaagggttg
      421 ggctgttcgc ccattaaagc ggcacgcgag ctgggttcag aacgtcgtga gacagttcgg
      481 tccctatccg tcgcgggcgt tggaaatttg agaggagctg tccttagtac gagaggaccg
      541 ggatggactt accgctggtg taccagttgt tctgccaagg gcattgctgg gtagctatgt
      601 agggaaggga taaacgctga aagcatctaa gtgtgaagcc cacctcaaga tgagatttcc
      661 catttcttta agaaagtaag atccctgaga gatgatcagg tagataggtc aggagtggaa
      721 gtacagtgat gtatggagcg gactgatact aatcgatcga ggactta
//
LOCUS       DAFEPQ010000198          714 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.209, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000198
VERSION     DAFEPQ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.209"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
BASE COUNT          254 a           87 c          143 g          230 t
ORIGIN      
        1 tgtagaaata cgggtaaata ttttggtgaa tagttatcaa aaagaactca aagaaagaag
       61 acggtggcgg tatttattga aaggatgaaa aagtaggatt tgacatactg gtattcagaa
      121 agtgccgtca cttttaaaac aatggaaaag gtggagataa gatgaagaaa aaacaaacta
      181 aactatcatt agcagatatt taacacagtt aaccgcaact gaagatggtg ttgttgaatt
      241 tgaacaatct gaaattgcgg tagtagatga tcgttacctt aaaatgcctt acttctttga
      301 ccaagcaaag gtcatttgcc tttgtcacta cgatggggta cgtgatttta ggcgttaaaa
      361 agtagtgatt atatcaactt tacgagtaaa tataaattta gacatgaaac tgaattatcc
      421 aatggaaaat catggtttat aatctagaaa aattatagta atgattggat tatatatatt
      481 ctttcgtatt ttcgtacaac tgtagcttgt caaaattgag aaatttaacc gattaggtac
      541 tttacatcgt tatttgggta atttctaaaa catgaagctt accaacgaat aaactgagac
      601 tatcttatta attgatcaat ataagaaaat atctttgagg agatgatgta ctatgttttc
      661 gtctatccac gattggttag gtggtctttt cggcggtgga ggtaaaggtt ttta
//
LOCUS       DAFEPQ010000199          657 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.210, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000199
VERSION     DAFEPQ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.210"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..>657
                     /locus_tag="LP543_002859"
     CDS             <1..>657
                     /locus_tag="LP543_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL2322941.1"
                     /translation="QLPFLKRYFSHFSREIREAVEWIVIDMYAPYVSLVKKLFPKAQL
                     IIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQKHYQLGKQLKRYWKLLQKDERKLDYT
                     RRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQLYQDFLYAVKERDYVSFEELLTNNI
                     MLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKL
                     R"
BASE COUNT          218 a          135 c          116 g          188 t
ORIGIN      
        1 caactgcctt ttttaaaacg ttattttagt catttttcac gagaaattcg tgaagcggtc
       61 gaatggatcg tgattgatat gtacgctcct tatgtatcgc tcgttaaaaa gctgtttcca
      121 aaagcacagc tgattattga ccggtttcac atcgtccaac atattggtcg tacctttcgc
      181 aatcatcgaa tcaaagaaac gaaccaacta ttaaaaagca aagaacaaaa acactatcaa
      241 ttaggtaagc aattaaaaag atactggaaa cttcttcaaa aagatgaacg caagcttgat
      301 tatactcgac gcctttggcg acctggcttc aaagctcatt taacagagac cgatattgtg
      361 gatcgcttac tgaaagggag ccctgctttg cgtgtaggct atcagttata tcaagacttt
      421 ctttacgctg taaaggaacg agactacgta tcgtttgaag aactacttac caataatatc
      481 atgcttcccg aaggctatca gacgaccttg agaacgttcc agaaatttct gccacaaatc
      541 aaaaatgctt tacaacaaag ttattcaaat ggcccacttg aatgtctgaa caaccatatc
      601 aaagtgttaa aacgaaatgc atatggcttt cgtagctttt ataatttcaa attgcgc
//
LOCUS       DAFEPQ010000200          642 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.211, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000200
VERSION     DAFEPQ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.211"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            73..>642
                     /locus_tag="LP543_002860"
     CDS             73..>642
                     /locus_tag="LP543_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL2322942.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIIT"
BASE COUNT          225 a          119 c          119 g          179 t
ORIGIN      
        1 agacaactaa aaaagtcatt tgtgctacac tcaaaatagt tccgaccaaa gaattataag
       61 gagtgagtac aaatgaccta tacccatctt acaacggatg aacttgtaat aatagagtct
      121 tatttcaaaa tgaatcaatc tgttgctaaa actgcccatt gcttgaatcg ttcaagacaa
      181 acgatccata aagtatatct gttcttcaag caaggaaaat cagccttaga atattatcaa
      241 cagtataaga aaaacaaatc aaactgtggt agacgtccgc ttgttttacc cgaggaacaa
      301 tcagaatata ttcaaagaaa ggttgttcaa ggatggacac ccgatgtgat tgttggtcgt
      361 gcagcgtttc ctattagttg ttctgctcgt accatttatc gtatgttcaa aaaggggcta
      421 tttgattctt ctgacttacc gatgaaaggc aagcgtaaac cgaatggaca tcaagaaaga
      481 cgtggaaaac aaactttccg ccgctctatt catgaacgtg aaaaggatta tagccaattc
      541 tcaaatgagt ttggtcacct tgaaggtgac actatcgtag gtctgaaaca taaaagtgct
      601 gtaattacct tagttgaacg attatcaaaa gttatcatca ca
//
LOCUS       DAFEPQ010000201          635 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.212, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000201
VERSION     DAFEPQ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.212"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(77..508)
                     /locus_tag="LP543_002861"
     CDS             complement(77..508)
                     /locus_tag="LP543_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HBL2322943.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          220 a           83 c           72 g          260 t
ORIGIN      
        1 aggaatgtaa agtcttatct cttttcttaa ttgtacaatt agtattatct gcactacttt
       61 ctaattaaat aaatttttag ttattaactt gattaaaatc agagataata tcatttaatt
      121 cagtttgaag ggttgtaacg attaaatgga aattttcttc atctgtttta ttatatgggg
      181 atgttttcgt tatatataac aacttttctt tttcaatttt cattaataac tctgtcgaag
      241 tctgtacttt atcttgataa ttaaatgcag acataccact ttgtataatt gtaattattg
      301 ctgaagcaat tgaaacaatc aatagtaaag acctgtgttc ggaagcctga tcaattaata
      361 ttgggatact agctgataag actattttta taatatttcc gataccaatt actctattat
      421 aaaatcgaat ttcttttttt aatctatcga ttgtagaatc aatagattcg ataaacgctt
      481 tttgatccat cattttctct ccaatcattt tattaaaatc attaacaaaa atattataat
      541 aggatttaag taagttctaa ctaatcacat ataaaaataa aaaaacattt gtattttatc
      601 aaagttttat tatacttgtc aatttgtgta catcc
//
LOCUS       DAFEPQ010000202          615 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.213, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000202
VERSION     DAFEPQ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.213"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
BASE COUNT          273 a           88 c          100 g          154 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactctc gatgaagtag acctttgaaa actgaacaaa
      541 gtaagacaaa ccaaatgtgt agggcgtctt gattcaattc aagacaacaa acatttttaa
      601 caagcaagca atatg
//
LOCUS       DAFEPQ010000203          599 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.214, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000203
VERSION     DAFEPQ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.214"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..111
                     /locus_tag="LP543_002862"
     CDS             <1..111
                     /locus_tag="LP543_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBL2322944.1"
                     /translation="ISREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            124..510
                     /locus_tag="LP543_002863"
     CDS             124..510
                     /locus_tag="LP543_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322945.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
BASE COUNT          205 a           69 c          152 g          173 t
ORIGIN      
        1 attagccgtg aagtgggtta ttcagaaaga agcatacaaa ggatgaagtc ggaagctcta
       61 atagagtttg cagaagcgta taaacacgga agaataattg cttataaata gggggaccat
      121 ataatgtgga aagattatgt atcgttgaaa gagttgaaaa aagatcttgt tttcaaaaga
      181 attgtagaat ggtcagagag tgaattaatt ttagaagatg gaaccaaaat ggaagttgta
      241 tgtagcgaat cagattgctg cgcatgggcc gaaggtgaat tcaaaaatgt aaagcttgat
      301 gcagtgatta cagatataaa gatttttgat aaaggtaacc gtctctataa tggtgacgga
      361 catacctctt atgctgaagt cgttgtttat cataatcgta atgagatcgc taaagcagaa
      421 tgcacagcga atgatgggaa tggcggctac tattatagtg tttgtgcttt aaaggtcaaa
      481 gacaaacttt gtatagtgac tgacgcataa aaaaatggcg gttttttggc ggaatgatgg
      541 cggtttttag ccatttatca gtgatattat ggtagtgtcg aaagattagt gataggtct
//
LOCUS       DAFEPQ010000204          588 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.215, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000204
VERSION     DAFEPQ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.215"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            26..412
                     /locus_tag="LP543_002864"
     CDS             26..412
                     /locus_tag="LP543_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL2322946.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT          222 a           93 c          118 g          155 t
ORIGIN      
        1 ataaaaataa aaaaggagat ctttcatgaa aaaaatttta gggttgatcg ttatcatatt
       61 gattgctttt ataggatgga aaggttggga ctactatcag tctacttatg taggtaaaga
      121 ttattatgcg gtagtcaaag ctccaatgcc tgcagaaact gatatcaaag cagataatgg
      181 ggaagtaatt ggaagaggtt ttaaatacaa cgtagatgct tatgctgaaa acggtgaaaa
      241 aagacagtta gatttcgatg tcattacttc tggtgactct gccaatggtt ctgcttaccc
      301 cgaaggaacg atcttgcagt tgaaagcaag cgaaaagcgg atcatagaga aaaaagtaat
      361 taccagcgat aaggtaccct catctgtaaa agaaaaatta ggcctatctt aagctaagaa
      421 tatatcgcaa aaaagcactc gaagtgagtg cttttttgct tactgattct agaaaacaaa
      481 aaaacagaat taaccttcga agtcagcctc actgttctta aagtcctaaa acaaactaaa
      541 gagcaaggca atcaatcgat ttctatacaa acaagggact gtgacaaa
//
LOCUS       DAFEPQ010000205          563 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.216, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000205
VERSION     DAFEPQ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.216"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..129
                     /locus_tag="LP543_002865"
     CDS             <1..129
                     /locus_tag="LP543_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL2322947.1"
                     /translation="SGITMEKTKKGLLKQWDRKSHELFISILKELEEHENWYAEVE"
     gene            130..>563
                     /locus_tag="LP543_002866"
     CDS             130..>563
                     /locus_tag="LP543_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322948.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYP"
BASE COUNT          233 a           80 c          119 g          131 t
ORIGIN      
        1 tcaggtatta cgatggagaa gacaaagaaa ggtctcttaa aacaatggga tcgcaaaagc
       61 cacgaattat ttatcagtat tttgaaagaa ttggaagaac acgagaattg gtatgcggag
      121 gtggagtaat tggctgacat caaaggactt gatatttttg ggaacaacaa aggactcgcc
      181 aaaataaaaa aagaagcaga agcaaagaaa aagcagcaag caattacacc atctgaaaat
      241 aagcaattag aagtgcacca agttcaagaa ataaaaactt cctccaatgt tgaaagacag
      301 agtagaggaa gaacaaaaaa agttggcgca ccaatcagaa agtttgataa ggtttattcc
      361 accaagcatc ctttaaaact gtcgactttg ttaaatgcga cttccagagt tttagtagaa
      421 aagtatgaga ctaatatgac aagagatgaa ttgcttagaa aagctttaga tgagtatatt
      481 aaacagaatc ttacgcaaga agataagcaa gatttgttta atgacgtggt gaaagaacta
      541 gacttgttca gagaaaaata tcc
//
LOCUS       DAFEPQ010000206          545 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.218, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000206
VERSION     DAFEPQ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.218"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..>545
                     /locus_tag="LP543_002867"
     rRNA            <1..>545
                     /locus_tag="LP543_002867"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          162 a          120 c          158 g          105 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtt
//
LOCUS       DAFEPQ010000207          544 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.219, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000207
VERSION     DAFEPQ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.219"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            141..398
                     /locus_tag="LP543_002868"
     CDS             141..398
                     /locus_tag="LP543_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322949.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          232 a           67 c           76 g          169 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 ttta
//
LOCUS       DAFEPQ010000208          529 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.220, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000208
VERSION     DAFEPQ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.220"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(5..90)
                     /locus_tag="LP543_002869"
     tRNA            complement(5..90)
                     /locus_tag="LP543_002869"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="LP543_002870"
     tRNA            complement(107..178)
                     /locus_tag="LP543_002870"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="LP543_002871"
     tRNA            complement(190..262)
                     /locus_tag="LP543_002871"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="LP543_002872"
     tRNA            complement(280..361)
                     /locus_tag="LP543_002872"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="LP543_002873"
     tRNA            complement(369..441)
                     /locus_tag="LP543_002873"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="LP543_002874"
     tRNA            complement(445..517)
                     /locus_tag="LP543_002874"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          136 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactta
//
LOCUS       DAFEPQ010000209          524 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.221, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000209
VERSION     DAFEPQ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.221"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(65..514)
                     /locus_tag="LP543_002875"
                     /pseudo
     CDS             complement(65..514)
                     /locus_tag="LP543_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          154 a          108 c           66 g          196 t
ORIGIN      
        1 agccataaca ccgcagcatt cacaacacct tataattttt gtcaaaaaaa gtgttgccat
       61 tccacttttt taaatatgcg ttctctgctc ttaaacgaat aacttcttct tcaagagact
      121 catctttaag ttttttagga atgtttagct tggaattcat actaattttt ctgccccttt
      181 tttggctctc aagtgaagaa gcaccgcctt cttcatattg ctctatccat ttacttagag
      241 ttctatttga accgatatta aattttttag cagtttcttg gatagaaaga ccatttgttt
      301 ccatatattc tataacatca agtttaaatt ttgtagtgta gcttttgcca cctccaacca
      361 agccttccca accatgatag ttataaatcc ttacccaatg tctaaccagt gtacgattta
      421 tttgatattt atgtgcaaga tatttgtagc cgccttcgtt atttaaatag tctgaaacta
      481 cttttttctt aaaaacaaat gtatatttcc gcaaaaaaag cacc
//
LOCUS       DAFEPQ010000210          515 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.denovo.222, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000210
VERSION     DAFEPQ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.denovo.222"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..248
                     /locus_tag="LP543_002876"
     CDS             <1..248
                     /locus_tag="LP543_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322950.1"
                     /translation="MLDMPTKNPKTRKKSKFKVGDMVRCEAEEFVYPFRGYVKKILSN
                     SAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            325..>515
                     /locus_tag="LP543_002877"
     CDS             325..>515
                     /locus_tag="LP543_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBL2322951.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMM"
BASE COUNT          182 a           89 c          120 g          124 t
ORIGIN      
        1 taatgctaga tatgccgaca aagaacccta aaacacgcaa gaaatcaaaa ttcaaagtcg
       61 gtgacatggt ccgctgtgaa gcagaagagt tcgtttatcc gttcagagga tacgttaaaa
      121 agatactgtc aaactcagca atcattcgta ttgaaaatac aatgaagtgt gatcgttcga
      181 ctgcaaaaca caagcattat ttagctgtag cgagattagt tgacatggaa gtaatcaaga
      241 gcaaataaaa aaagccggat cgctccgact gattcaataa atccaacaca tttattatat
      301 cacataaagg agcggtttga cttgatgcaa ttgttacgag aggtagattt caaacagaca
      361 agatgtaatg cgagagatgt gctgaagaac tttcggcgtt tggagcggat ggcaggtcgc
      421 tctttgatag atattaagtc gccgattata acggatatgc cgaaggcacc gaagcatggc
      481 aataaggcag aagacgcgat tattcagatg atgga
//
LOCUS       DAFEPQ010000211        20985 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.guided.045, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000211
VERSION     DAFEPQ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.guided.045"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            complement(134..577)
                     /locus_tag="LP543_002878"
     CDS             complement(134..577)
                     /locus_tag="LP543_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBL2322952.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="LP543_002879"
     CDS             complement(795..2135)
                     /locus_tag="LP543_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2322953.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="LP543_002880"
     CDS             2404..3300
                     /locus_tag="LP543_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBL2322954.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="LP543_002881"
     CDS             complement(3450..4391)
                     /locus_tag="LP543_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBL2322955.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="LP543_002882"
     CDS             4517..5638
                     /locus_tag="LP543_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL2322956.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="LP543_002883"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="LP543_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBL2322957.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="LP543_002884"
     CDS             7426..7992
                     /locus_tag="LP543_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL2322958.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="LP543_002885"
     CDS             8203..9264
                     /locus_tag="LP543_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBL2322959.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="LP543_002886"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="LP543_002886"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBL2322960.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="LP543_002887"
     CDS             10177..12159
                     /locus_tag="LP543_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL2322961.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="LP543_002888"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="LP543_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBL2322962.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="LP543_002889"
     CDS             12881..14305
                     /locus_tag="LP543_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2322963.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="LP543_002890"
     CDS             complement(14486..15259)
                     /locus_tag="LP543_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL2322964.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="LP543_002891"
     CDS             complement(15256..16161)
                     /locus_tag="LP543_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2322965.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="LP543_002892"
     CDS             complement(16315..16635)
                     /locus_tag="LP543_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBL2322966.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17109..17795
                     /locus_tag="LP543_002893"
     CDS             17109..17795
                     /locus_tag="LP543_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBL2322967.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17797..18741
                     /locus_tag="LP543_002894"
     CDS             17797..18741
                     /locus_tag="LP543_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBL2322968.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            18940..19584
                     /gene="pcp"
                     /locus_tag="LP543_002895"
     CDS             18940..19584
                     /gene="pcp"
                     /locus_tag="LP543_002895"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBL2322969.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVSFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            19663..20940
                     /locus_tag="LP543_002896"
     CDS             19663..20940
                     /locus_tag="LP543_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322970.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6779 a         3783 c         4361 g         6062 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaaaaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttctata tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat
    16981 aatttttgtg ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa
    17041 acaactatat tttttatgtg ttttgcttta taattaaaaa aatattgtca ttttatggag
    17101 gaaaatatat ggaaaattat cttagccttt taggcgtctt gattgttatt ctcgggtttg
    17161 catttaaatt agactcgata ttgatcgtaa tggttgcttt agttgttaca gctttgactg
    17221 gcggtcttgg cattcaagga atgttagaac ttttaggtac aagttttgta aacaatcgcg
    17281 ggatggcgat ttttatcatc attatgttgg caacaggaac gcttgaaaga aatggtctga
    17341 aagaatcagc ggcttccttg atcaagcgat tcaagaaagt atctgctggt atgattgtag
    17401 atatttatgg gatctttcga atgatctttg cggcttttaa cgtgagtttt ggtggagttg
    17461 ctggatttgt tcgtccaatc attttaccga tggcattggg gaccatcgaa tcaaaagggt
    17521 tagaagtcca tcctaagcat gaagaagaac taaaaggtat ggcttcagct atggaaaatg
    17581 tctgctggtt ctttggccaa gtactattca tcggaggagc aggggcgttg ctagtccaat
    17641 caacactgaa agatctagga tatgacgtaa cattgggaca gcttgcttta gtggaagttc
    17701 cagtggctat cacggcttta gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat
    17761 tagcgaagaa atattatcca ggaaaggggc aataagatgg acttttttac aaatcctgat
    17821 attttattag gcgataaatt actggagatc ctttatatcg tgatgggact cgtattgatt
    17881 tacactggag taagaaattt aacagataaa accaatcctc atcgttatgg gtcagccttt
    17941 ttctggacgg tcttaggggt agttattgca ggaggaagat ggcttccggc aatcgtgaac
    18001 ggtggcttga tctttgcgat gaccatacca gcaattgcta agaaagtcag caaaggagaa
    18061 agccggcttc catcaaaaga atatatggaa aaaatgtcag acaaactagg aatgaaaatt
    18121 tttattccag ctttgagtat cggtgtcttt gcgatcttgt ttgctctatt taccaattta
    18181 ggtgctttag tcggcgtggg cgtcggtgtt tttgcagcca tgctaatctt gatgttcttt
    18241 tctcgagata atcgaccaag tacattttta gatgatgcag cagatatgct gggcacagta
    18301 gggccgttaa gcatgttgcc aatgctttta gcttctctag gtgcggtatt tacaagcgct
    18361 ggagttggga cagtgatttc caatgcagta ggtacgatcg ttccagaagg aaatgtgaac
    18421 atcggcatca tcatttacgc tcttggcatg gtgatattta cggtaatcat ggggaatgca
    18481 tttgcggcca tcaccgtgat gacggttggg atcggtgcac cattcgtatt tagtcatgga
    18541 gcgaatccag cgttaatagg aatggttgca ttgacttgcg gtttttgtgg gaccctgctg
    18601 acaccgatgg ctgctaactt caatatcgtg cctgtagcga tgctggagat gaaagacaaa
    18661 tacggagtga tcaaaaacca gttgtttatt gcgttattta tgctagtttt ccaaattgct
    18721 tatatgattc tatttaaata atagaagaga ggggttgtga cagaataatt gtcgcaacct
    18781 cttctaacat tgtagtggaa cactattttt ttataagaat atattaagtt tgatctgtta
    18841 tgttatttaa actattgttt cctttattta cgctgttcaa ggaaaatggt atgataaaag
    18901 ataaccatat gttaaatcaa tcggacggag gaacaacaga tgaaagtact agtaacaggt
    18961 tttgatccat ttggtgggga caaagtgaat ccagcctatg aagcagtaaa aaaaatgcca
    19021 gatgaaatca gcggtgctga aatcatcaaa gtggaagtac cgaccgtatt cgaaaaaagc
    19081 agtcaagtag tcaaagaagc catccaacag caccaaccag atgtcgtgat ctgtgtcgga
    19141 caagcaggtg ggcggtcagc agtctcgttc gaacgtgtcg caatcaatct agcagaagca
    19201 agaatccctg ataatgaagg aaatcagcca ttcgatacgg ctttagaaga aaacggtcca
    19261 gccgcttact ttacctcatt gccaatcaaa gcaatgacaa aaaatgtaca agatcatggt
    19321 ctaccagcgt acatctctta tacagcagga acatttgtct gcaatgatat catgtatcgt
    19381 ttgcttcatt tgatcgaaac acaattcccg gcaattcgag gtgggttcat ccacgtgtca
    19441 ttctcacccg atcaagtgat cgatcgtcca gtgggtacgc cttctatgtc tttagaagac
    19501 attgcttctt ctttgactta tgcagttgaa gcagcagtag aaaataaaga agatatttcc
    19561 ggaaatgccg gaacaactca ctaatttagc aagaaacact ggctggttgc taacgttatg
    19621 ttagtagcca gctttttgta attagaaagg aaagaaacag ccgtggaaga atggcaatcc
    19681 gtctttgaag aatggtttcc aaaagaaatc agcaaaagct atcccatcaa aatttctaaa
    19741 caatacacaa gcagtcaacg ttgggagatc tatgcaaaat taacgaaaaa acagcgggaa
    19801 ctagtcgata agcaccgccg atatctaatc agctctcgtt ttatggaaga acattattta
    19861 gctgcaacag attgggtgtt ttctgacttc aagatcaatc cgttttttcg aacaaaacga
    19921 agccagcaaa aattgtattg tgagtgtggt agagaattaa aagtgcaata catcgttaaa
    19981 tcgccaaaaa ccggaaagat cctaaaacta ggcatcaacc actttgccga tcacctgcat
    20041 gtctctccga cagtggcagc ttcgatccat cagggaatga caaaagttga tctggcctta
    20101 gacgaattat tatggcttaa gcagaagaat atcgattttc ctgaaggact atggcaaaaa
    20161 tactgttttg tcttgtacca aaatcgtcgg atgaaacagc catatttacc ggatataaaa
    20221 ttagctcagc ggctcgcaga atttcgccaa gtagaaatgc cgatttacat agcggattat
    20281 caagcattgg aaaatgaaat caagaaaata agtgaacaca tcaatgggca gtcaaaaaag
    20341 cgacagataa aaaaagaact atttgacgac tttgcagaag aattagtaaa ggatgtagaa
    20401 gaattcttga tcaactatcg cgcattttta cgtaaagatt ggcaatccat cgtatatgaa
    20461 gaagtgccag tacatcctaa tgcttatttt gaaacattta tttccgtgtt gagaaagaca
    20521 aaacgtcaac gaacaccaga agtcactgct cagatggaat attttgcgaa aaaccaacga
    20581 ttcatccaac caaaaatcta tctatttatt tggaagcaat attgtcgcta tggttttaca
    20641 gaagggttct tcgacagcat tcctcggata gtcagaaatg gttttttaaa agtattgcgt
    20701 aaagaacgag aagctataca atctgctgat aagaaagacc gtacagtctc aaaagaaaaa
    20761 tggcaactag tagtgaaaga tatccaatct ggaaatgtgc aagagacaat cgacaaatgg
    20821 aaaggaaaac attatcgttt tactgaagct cagaaacaag cgttggaata ttatcagaag
    20881 ttagaagaat cactgcgttt caatgatgag gcaagaaagt atctgaagga attactataa
    20941 aaaatagagg ctgggacaga agcgtttaac tccaagaaat aagaa
//
LOCUS       DAFEPQ010000212        20863 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.guided.046, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000212
VERSION     DAFEPQ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.guided.046"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            267..1304
                     /locus_tag="LP543_002897"
     CDS             267..1304
                     /locus_tag="LP543_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL2322971.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(1393..1716)
                     /locus_tag="LP543_002898"
     CDS             complement(1393..1716)
                     /locus_tag="LP543_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBL2322972.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(1703..2410)
                     /locus_tag="LP543_002899"
     CDS             complement(1703..2410)
                     /locus_tag="LP543_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBL2322973.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2496..2837
                     /locus_tag="LP543_002900"
     CDS             2496..2837
                     /locus_tag="LP543_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HBL2322974.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(3008..3856)
                     /locus_tag="LP543_002901"
     CDS             complement(3008..3856)
                     /locus_tag="LP543_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2322975.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            4454..5317
                     /locus_tag="LP543_002902"
     CDS             4454..5317
                     /locus_tag="LP543_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBL2322976.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            5417..5899
                     /locus_tag="LP543_002903"
     CDS             5417..5899
                     /locus_tag="LP543_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBL2322977.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(6162..6629)
                     /locus_tag="LP543_002904"
     CDS             complement(6162..6629)
                     /locus_tag="LP543_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBL2322978.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            6752..7603
                     /locus_tag="LP543_002905"
     CDS             6752..7603
                     /locus_tag="LP543_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL2322979.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(7854..9812)
                     /locus_tag="LP543_002906"
     CDS             complement(7854..9812)
                     /locus_tag="LP543_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2322980.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(9809..10681)
                     /locus_tag="LP543_002907"
     CDS             complement(9809..10681)
                     /locus_tag="LP543_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL2322981.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(11023..11895)
                     /locus_tag="LP543_002908"
     CDS             complement(11023..11895)
                     /locus_tag="LP543_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL2322982.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            12023..12592
                     /locus_tag="LP543_002909"
     CDS             12023..12592
                     /locus_tag="LP543_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBL2322983.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12659..13396
                     /locus_tag="LP543_002910"
     CDS             12659..13396
                     /locus_tag="LP543_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL2322984.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            13627..14895
                     /locus_tag="LP543_002911"
     CDS             13627..14895
                     /locus_tag="LP543_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBL2322985.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            15382..15846
                     /locus_tag="LP543_002912"
     CDS             15382..15846
                     /locus_tag="LP543_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL2322986.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            15830..16459
                     /locus_tag="LP543_002913"
     CDS             15830..16459
                     /locus_tag="LP543_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL2322987.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            16813..18291
                     /gene="msr(C)"
                     /locus_tag="LP543_002914"
     CDS             16813..18291
                     /gene="msr(C)"
                     /locus_tag="LP543_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBL2322988.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            18338..18784
                     /locus_tag="LP543_002915"
     CDS             18338..18784
                     /locus_tag="LP543_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBL2322989.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(18983..19498)
                     /locus_tag="LP543_002916"
     CDS             complement(18983..19498)
                     /locus_tag="LP543_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL2322990.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            19615..20817
                     /locus_tag="LP543_002917"
     CDS             19615..20817
                     /locus_tag="LP543_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2322991.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         6632 a         3703 c         4315 g         6213 t
ORIGIN      
        1 caaggagcaa tttcaaaaaa cagcttctca tgtttgatga atattttgac ttgtgaccga
       61 agagttggta ggagaacacc gtttataaga ggttgtgaca aaagtcttgt cgcaacctct
      121 tttttaacca aaaaagtgca aatagtttac tttaagtaag agaataatta tgatgaataa
      181 aaaaggtgga acaaatagca tactatatat attgaaatga acactgtatg gatgctccaa
      241 cttgatgaag ctaaggagga attcgtatgt cagaaaagga attaatgaag gtagtcggtt
      301 tttatgaagg gttgccgatc gatgatccaa aaagctttat tgatgaaaaa gaacagatac
      361 ctgtacccag ttcacgggat cttctggtaa aagtcaacgc agtatctgtt aatcctgtcg
      421 atacgaaact tcgccaagat aacgggataa gaaatgcttt acgtatctta ggttttgatg
      481 gagtcgggaa agttgttgct gtaggtgacg aagtgcaaaa attttctgtc ggcgatcgcg
      541 ttttttacgc aggtacaaca acaagagcag gaagcaatca agaataccaa ctagttgacg
      601 aacgtattgt cgctcttgca ccaaaaaatc tttcagatga agaagctgca gccttgccgt
      661 tgacctcatt gaccgcttat gaattgttat ttgaaaaatt tggtctaact ccagaagaaa
      721 atgccaatcg cgggaaaaaa atcttagtga tcaacggatc cgggggcgtc ggttcgattt
      781 tgaatcaact agcgcattgg gctggcctag aagtctacgc gactgcaagt ccgaagaatt
      841 ttgaatggct gaaaaaaaca ggtgtagact atccaatcga ttatcatcac gacttaagta
      901 aaagttttcg tgaattagga atcgatcaag tagagtatgt cgctgtatta ttcgatatta
      961 cccgctactt tgatcaaatc aagcaccttg tccgaccatt tggacatgtg ggaacgatcg
     1021 tagggatcaa agagccgtta gatataggtg tttggaaaaa ccaatctatc agcttcgact
     1081 gggagtatat gttcgctaaa acagactata actatgaaat cgaaacgcaa ggagaagctt
     1141 tagcgcacat tgctgcttta gcaaatgaag ggattatccg cagtacattg agcaaggtct
     1201 attcagatgg gatgaatgcg aagaacttga aacaagcaac caaagacgta gaaacaggac
     1261 atatgttagg aaaagtcgtt gttagtggtc cgttcaacgg ataagcaaga atacgccgat
     1321 ttttttctta tttgactttg ataaaaacaa gagcgcatga cagtaatttg ccataagctc
     1381 ttgttttttt atttataata agcgaatcaa cgaaagggaa agaattcctg ccaagacgat
     1441 caccaataag ctttttgaaa gaatagcagc taaaagtgta ggaactgaag ccaaagcatt
     1501 ttcgagattg atctgcggca gatgtccgat attttgggta aatagactgc tgaaccataa
     1561 agcagacata atgacgatcg ggacaaaact taaatactca agcaacggtt gcggtaaatc
     1621 gaattttttc agtaagacaa atggtaggac cctagaaagc caagttgcta aagtacaacc
     1681 taaaatagtc agcaatgtaa actcaaaaga aggcatgttt gatccacacc cccaatccgc
     1741 acccaatcag tgttacaact acaatcacta agttgcttgg gataaaaatc agtccgacat
     1801 ataccagaat caaagtgaga ccaatcagga tcaactgtaa tcgtttagag gtgtttctat
     1861 cagaaatgac ttgaagataa agtaacccga taaacatagc gacgatggcg aaatccaaac
     1921 caaacttttt cggatcagta ataaaattcc ctaaaagtgc gccaacaaga gaagaaccca
     1981 cccacgtcaa ataagaaatc caattagctg tattcatcca acgaaagttt aacttttgtc
     2041 cagtatagtt caatttattc attcccagtg cgaaactttc atctgtcagc aatgtgccaa
     2101 tcagcagatt ttgaagcagc cgatttttct tgaaataagg agcaatcgtc atactcatca
     2161 gaatcattcg ggaattgact agaaaggtcg agaaaacgat ggaaagcaat gggctgtgac
     2221 tagcaagcat gctgactgtg ataaattggg cagaaccagc atagaccagc agggacatca
     2281 acgtgacgac tagtggatga aatccggcag ctttcccaac gataccaaat gcaagtccaa
     2341 taccgatata accgaaaacg gtaggtaacg tgtctttgat cgcagttttt atatctaact
     2401 tttcatccaa taacaaaact cctttagcac gacataaatt ttcataattg tcgttaaact
     2461 tgtgaatatt ctaaattttt aatgagcatt tgtcaatggt gggattgaaa cgtaaagaac
     2521 tagcgggtcg cagatttaca cgtattgttt cggtgggagg aggcgcgaaa aatcttgcat
     2581 ggctacaaat ccaagctgat attttcgatg caacggtcta ttgtttaagt gtggaggaag
     2641 gacctgcatt aggcgcaaca atgattgctg cactagggtt agggtggttc gacacacctg
     2701 aaagttgttg tcagaatttt atccaatata cagatgcagc ggaacctctc ttacaaaatg
     2761 tagaaaagta tcataaaatc tatcaacaat gccggaaaat ctatccagca acgaaggaaa
     2821 tttgtcatga cttataaaac aaaacgcgta cctaatagtc aaatgactaa tattgggtac
     2881 gcgttaagtc ttctttactt catcctttgc atgattcgtg tttttaaagc aaggtctgct
     2941 gtatttcttg tggatttagg atggttgtta tcgtttactt gctatttttc attgaaatcg
     3001 tctttcttta ccgtttcttt agttttttat ctattttttt cttttggtta gaaaattctt
     3061 tttcccgaaa gtcttctttc aggattttta tagagtagcg ttttgaataa gaggcgtcag
     3121 tatgatcgac ttggcagacg aaccaagaga acatttgcct tttttctgca tgaggtgctt
     3181 ctgctaataa ttctttccat aaataataac agtcctctat cagaagaagg gttcgttcat
     3241 cctcaggctc ccaatagaat gtttcgagag tagcaatcac ttcattgatc aattcgaatg
     3301 cgggaagata ggcattttgc tgaataagcg attcaactgc ttcttggatg aactttcgca
     3361 tctccatgat gaacttcgct gttttcctgt ttttgttttt cttgataata tgatcgtatt
     3421 tatggatgat agttgttaat ttatctgaat aatttattgc atgaaatggc atcttaactt
     3481 tgaagcgttc gataaagtgc ggatgttcta taagaatctc tgttaaaagc tcccgtacaa
     3541 gagaggaact agcggaggaa acaagggacg agagttcgga aagtgagtag ttggattttt
     3601 ttattggacg ttcttcgtaa gcgaataaga cagcagccat gtgtttgcat ttttttcctt
     3661 ctatggcata tagacaatca caagtcatgt tggtcttttc atcccaagtg atcactacct
     3721 tataatcttc cgtgccagaa actgttgcgg acaagtactt gcttgtatag ttcaaatgac
     3781 ggacaaaacc gtgcagatga tagctgtaac cgcgttccaa aacgttttca ggaaaatact
     3841 caagccaatt attcatctag ttctcctttc atgaaacact tatttatatt tagtataaag
     3901 aaacgtttaa gaaaaaagta gctgatttta catatttgat aatcactttt gctcgaattg
     3961 tcaaaaattc attctccttt tcttttctta gagaatgtga gaaaaagtaa aagaatagcg
     4021 tgttttaaag atggaacttt ttttgttttt tatttatctg acaaaaatga agtgattttg
     4081 ttagaaataa ataattgttc caattgataa aagaggtatg atgtatcgca aagaaaaatc
     4141 caagcagcgt tctgatcagc agtttgaaca acaatttgct ttgtacagta ggtagtaagc
     4201 ggattaacta agggaataaa agatattttt accgctttgt ttacttagaa tctgtcttcc
     4261 ccaaaattta tttttctaaa ggtaaaacat agataggcag ataataccaa ttatccgtta
     4321 cgaaacgctg cttgagaatt ttctttagat ccatacaaca ttttctacat ctgactaaat
     4381 tggttcctga ttcatcaagt aattgacttt ctttgataat atgataaaaa ggcaatcaag
     4441 gaggaagata aatatgctta aagatctgaa ccaattgatc gattacatcg aagaacattt
     4501 gacagaagga ctttcctata aaataatagc cgaaaataca ggaatctctg aatatcaatt
     4561 aaaacgcatc tttacgttta ttgcagggat gtcgctaatg gactatatca aaaaccgtag
     4621 attggctttg gctaatcgcg atcttctaga tggatcaaga gtgacagaaa cggcgtttag
     4681 atatggatat gaaacagtcg aaggtttttc aagagctttt cgtgattggt caggatatct
     4741 gccttcagaa atcagtagat acggcataca aaaatctttt ccacgcttga cattttttat
     4801 tgatgtcaga ggaggaaaat cgatggaatt tcgtatcgaa aagaaagaac agttttatgt
     4861 ggcaggtgtg gcgaaaaaag ttcctgtcaa atttgaagga gtcaatcaag aaatacaaaa
     4921 gttagcgcag acgatcacgg agactcaaag ggaagagatg cataagttag gagatttata
     4981 tccaaatcaa gtaatcaacg tttcttatgg atttgatgga gaccgcttgg aagaaaaggg
     5041 cagtttgatg cacatgattg gctttgctac gacaaaagaa aatccgtacg ctgatttaga
     5101 aatgcttgct atagaagaac acacttgggc catttttcct aataaagggc catttcccca
     5161 aactttacaa gatacttggg ggaaaatcta tgcagaatgg cttccttctt cagattatgg
     5221 gttagtcgaa gctcccgaga tctcttttac aaaatgggga gaggatttca gtaatgttta
     5281 cagtgagatt tggattgcag tgaaagcaaa aaaatagtta aaaaataaaa atatccctag
     5341 aaaaaagagc gtaatcgtgt catttccctt taattttatg taagatgaac ttaatataat
     5401 agaaagggtg ggagaaatgg caaagtatag gatcgaagaa aaaccagatt ttacggtgac
     5461 gggtattggt gtggaattga agagtagcaa tacggatttt gtcggtttgg ctgcagagaa
     5521 acaagcattt gagcaaaaag ttatcggtaa tggaacaata gatgcattga aacaagctgc
     5581 tgaaaatcat tatttcttta cagtcaacga accctataac gggaaaatga tgtactatgt
     5641 cggtgttcaa acgagtcagg atgttccaaa cactacgcga atcatcccat tcccaaaagg
     5701 aacgtatctt gttgtaagcg ctttagcaga atcgcctgaa gaattaagaa atgatttgac
     5761 gaatacggcc tttgggcatg ttttaagaga agtagaagat tacgcctatg ttgggggtcc
     5821 aaatgctgta gtcgaaatgg gagttaccaa cgggaaatat atgggagaaa tctggatacc
     5881 ggtagtcaat aaaccatgat aatatagagg tcgtgaaagt agcgctttga cctgagcatt
     5941 agagaggaaa aatgggaaat agctttccat attttgcatt tttcagaact aatgccgagg
     6001 gtcagctact tgaacaccgt ttatcaagac gtcatgactt cagcgctttg acctgagcat
     6061 tagagaggaa aaaggggcat gacaaggctt ttgtcccccg gagaataaaa gaatttatgt
     6121 agacgattcc gatcagaaaa atgcaggcac agtagagaga attataaaaa tctctctacg
     6181 aggcctgtgt tttctggtct tttttctttt tccaaaacgg ctattttatg tatcagacta
     6241 tcaagtgtga tcttagccat ttcttcttct gcctttttct gcagccgagc gtagctttct
     6301 tctaaagctt cttggatatt cgcaccaacg acgcaatttg gatttgtttt tggatcgact
     6361 tggataagct gtgtattacc ttcgatgctg cgatagacat ctagtaaaga aatctgatct
     6421 ggacttttgg caagggaagg ctgcggtttt ccggtttttg taaagatcaa accggatttt
     6481 ttgagctgac tcatgatttt acgtacattt gctgcatttg tttcgatact actggcaatc
     6541 atctcactag aaagtgatcg ggtatcttga aacatttcaa tataggcaag tacatggatt
     6601 gcatcactaa attgcagaga atacttcatc agttgtcatc ctttcatttt ttttgcttga
     6661 cttatcttac cacactcttt atgataaagg tgtaatttat aaatgtacag ctgtaaatct
     6721 taacaaataa aaagaatagg gagtggcaaa tatgcattat ttagtaacag gagcaactgg
     6781 aggttttggt ggttatgcgc tggaattctt aaaaaagatg gttccacaaa atgaaatcta
     6841 tgtactagta cgtagtgaag aaaaaggaga agcactgaaa gaagctggat ttaatatacg
     6901 tattggtgat tatgatgatt tagactcaat gaaagaagcg ttgaaaggaa tagatcgatt
     6961 gctttttgtc tctggcgttc caggaaatcg tcaagccgaa cataagaatg tagtgaaggc
     7021 agcacaagca gctggggtat cttatatcgc ttatacaagt tttgcagatg ccgatcactc
     7081 gatcagtata ttagcgccag atcaccaatt cactgaaaaa atgattaagg attcaggtat
     7141 ttcccataca tttttacgga ataattggta tgtagagaat gaattaccta tcattgggca
     7201 ggctttaaaa actggtcaat tcgtttatgc tgcaggaaat ggaaaaacag gttgggcatt
     7261 gaaacgtgaa tatgcagaag ctgcagcaaa agtactagta gaaagtgact ctccagaaat
     7321 cttagaatta tccggcaatc taactgacta tgaagaatta gccaaggcat tagagcgtgc
     7381 gacaggtaaa gagttagaaa ttattgaagc aagtgatgca gcgtttgtcg aaaatctaaa
     7441 agaagccgga ttccctcaag aagcagctga catgttttta gcgatccaac atgatattaa
     7501 aaaggatcag ttagctattt cctctgatga tttagaaaaa gtattgggaa aaccattaac
     7561 ttctcttgaa gcagcattaa aagaaatatt ggatcaggaa taatgtataa agaggttgtg
     7621 aaaaagccga ttagttttga gtatgaagaa aaattttgtg aaaaagctgt tctgcatcaa
     7681 gtaataagaa gagtcaaata aaaaatagct cttcatatgt ttcgtttagc tggacttatt
     7741 accgcggagt tggcaggaga acgccgtttt tattcggaaa aaggagctga gacaagattt
     7801 ttgtctcagc tcctgtttgt ttatcgataa aaaagtctgg ggaagacgat ggctcaggct
     7861 ttgttggaaa cagagccgat tttatcattt gcttttacat cacgtgcggg ttcgatttct
     7921 aagctttgga ctttgtcgcc atttgtgaat acaacgacca tatccgtgtt tttgcctgca
     7981 gattgaatag cggcaagatc aactaaagca atcaattgac ctcttgcgac tttttgacct
     8041 tcttctacat ataaagtgaa tggttcgcct tttaagtcaa cagtgttgat gcccatatga
     8101 agcaagactt cgataccagc atctgtttgg attccgacag catgtttcgt tgggaaaatg
     8161 tttgtgatcg taccagcgat tggagagaag atttctccat tttctggtaa tacagcatag
     8221 ccgtcaccca tcattttttc cgcgaataca tcatctgaaa cttcagtgat tggaatcagt
     8281 ttcccatttg cgatagcgta aatatcttgt tcgatcgttt caccggcaaa tgtttctgtt
     8341 ttaaccacgg tgtttgtttg tgcaggtcgt gctgcagcac caccatgtag ttttgccatc
     8401 tcatctgcaa cgaattgtac ttctgtacct acgatgactt gcgcattttt tccgtcgatg
     8461 atcttcatac caggtacgcc agtggctttg attttatttt gatcgatcag gcctgtatct
     8521 ttgacttgca aacgaaggcg tgtagtacag ttatcgatcg aagttacatt gtcggctccg
     8581 cctaatgcag cgtagatttt gcttgcttgt gccgcatatt tattgtctga attagtcgtt
     8641 gcagtatcgt catctgaaag atctgcttcc tcgcggccag gagtcatcag atggaatttt
     8701 ttgatagcaa agttaaagcc aaagtagtaa atcactgctg ttactaatcc ttggacgatc
     8761 aacatcaatg gctgattcgc aatcggattt ttcaaagata ggaagtaatc gacaaatccg
     8821 gcactaaagg caaatcctgc tgtccaatgg aagaatgcac tcactgctaa agaaatccca
     8881 gtgaaaattg catgcaagac atataatggc caagcaacga acataaatga aaattcaagt
     8941 ggttctgtca caccagtaaa gaatgaagca aaagctgctg ctagcatcaa ggaagctgtt
     9001 tttgctttgt tttctggacg tgcatttctg taaatagcat atgctcctgc tggaagcccg
     9061 aacatcatga ttgggaaaaa tccagcttga tacattcctg ttacgccttt tgttccttca
     9121 gaagccaaaa atttaccaat atcattgatt cctgctacgt caaaccagaa cacggagttc
     9181 attgcttgat gcaatcctgt agggatcagt aaacggttga agaagccata aagacctgca
     9241 ccaccccagc ctaaactcaa aatgaattta gcaaaagaaa ctaaagcaga gtaaaccgga
     9301 ggccatacga gcagtagcac tgcactgatc agcaacatga caaaggcagc caagattggc
     9361 actagacgct tgccgctaaa gaaagataaa gccatcggta attttgcttc gtggaaacga
     9421 ttataaagag ctgccgcaat caatccggca ataataccaa taaatacgtt actgatctga
     9481 gcaaaagatg gatcaactga ttctggtttg atattcaata aagtagctac tgaatcagtt
     9541 tttaaaacat ttactggaac ttcaaatgcg accaaacctg ccaaggcagc agcacctgat
     9601 ttgtccactg acatccctag tgctaaccca acggcaaaca gcaaaggtaa ttgacctaaa
     9661 actgcgttac cgccagcttg taaaaaatga gcgacaatgt cgttactata agatgcccac
     9721 cagtttccga tccctactag taaagcagca gcaggtaaga ccgcaactgg aagttgaagg
     9781 gaacgtccca ttcgttgtag atatgctttc aatttttttc ctcctcatga ttgttaacat
     9841 ttgtttgtac gattgcgctt cttaaacgct cgatatggat ggccaagtaa cccagttctt
     9901 ctttcgtgat cgcgagctgg tattctttgg tcagcaacac acggattttt ttagaaatct
     9961 tatagctttc tgggtatttt cgtttgacca tttctacgat ttccgtgtct aaaacagcat
    10021 attgttgcgc atgggttcgt tgtagaagca gtcgtaaatg attcaccagt cttgaataat
    10081 tcagtgaaaa tggttctgaa tggatatctg tttccaattc tgattccact aaatggatga
    10141 tgtcagatac aatcgtcact tcacggatac tgcgatggtt attgttccga ccgcttcgtc
    10201 cgctgtgaat atggattgcg atatatcctg cttcgtcata agtatatgga atcccaagct
    10261 cttttgtcag atactctact gcccattgag caatactgaa ttcatcactg taaagcactt
    10321 cgatttctct taaaagtttg ttgcggatga cgataccgtt attaatgttc tccgcagaaa
    10381 aagcaatgtg gtcggtcaaa gcgatcaata catgttcatt caacttttcc atcaaaacag
    10441 tttccgcatg atcaatgatc gtttctgctg caaataaaaa gcgttcatca atctgattaa
    10501 gtaaattttg caacttgatt tgaccttctg gttccatgac aaatatccgt tcgacatcac
    10561 gatcaaaaat caaatcattt ctttttctgt caaatccaat cccttttcca atagctactt
    10621 tttcctgtcc accgtcatct accaatactg cattttgatt aaagatcttt ttgattttca
    10681 tgcgcgcacc ctttttgaag atttggtttt cacgtttgct agaccagttg attgcggcca
    10741 aacaaaaata gacacgaaaa acctcctagg gaaaccatgt tcctcagaga tttttcatgc
    10801 ctagtatgat ctagtcacat gaaacgaatt attgttaacg taatcaattt tatatagaca
    10861 gttatgtttt gtcaattctt tttttataat tatagtaatt tactggaatt atcagaaata
    10921 aaaggaagaa ataaattaag aaacttatgt tatttaatcg ttggatcttt tgcttatctt
    10981 attcgttgtt aaaaagctga aagcgaagga aaaaaactct acttaactgt atttctccgt
    11041 taggacatct aaaaatttac gagcagcagg agaaagaggc tggtcttttt tccaaatgag
    11101 attcagtgaa gcttgtaatg gcggatcgag tgggatgaat attttttccg tcccataagt
    11161 attgatgatc ccatctagag ctaatgcacc aaaagtcccg ttatcgacca tcaaagaagc
    11221 attgtacaat aagttatatg ttccgacgat cgataactga tctatgctga aacctagcca
    11281 ttcagctagt tgactgtcca ctgacgtttg ccgggaaatg atcaaaggaa cagttttcag
    11341 ttcctgcgga tggatgacgg tttgctttgc taaaggatga tttcgatgta tcaaaagtcc
    11401 ccaacgatct tgggctggca gtcgaaatga ctggtatttt tgtttgtttg tcggttcgat
    11461 tacgagtcca aaatcaagta atccgctatc cagtttgtct atgatatcat ctgcattccc
    11521 gctatataaa tgaaactgga tatcaggatg cgccttgatc aacgaatgaa ggctatcaga
    11581 aatgaaagag attgctttag tttcgcctcc gccaatgtag atttctccag agatgattgc
    11641 gtcatttgtt aaattgttcg ccgttttttc taccaacgac aagatttctt ttccttttga
    11701 ataaagatat cgaccagaat ccgtcaactg tatctctcgg gagccccgag tgaaaagtgt
    11761 cacaccaagt tcttcttcta aatctttcag ttgttttgag agagtaggtt gcgtgatatg
    11821 cagagatttt gctgctcggc tgatattttt ttctctagct actgctaaaa aatacgtcag
    11881 tatacgaata tccatttttt ccctcctgtt tttaaaaaag cataccacta agtatagtta
    11941 aaaggaatag ttaagtatgt tttataagta ttagacatga taagccgtcc gttataaagt
    12001 aaggataaaa ggagggagca aaatgacaag cctcatcgaa aaacaaatcg tcaataaaga
    12061 gattcgagta acggatccgc tctttgaagt gatccatcag atccaagctg aaaacgaaga
    12121 gcctttagca gcgctgaata ctgggtacca tgaaccagca gatatccgca aacggctaga
    12181 aaatatcatt agtgacaaag tagatgatac ggtgactgtt ctgctgcctt tttatacaga
    12241 tttcggcaaa catattagta tagggaaaaa tgtctttatc aatcgtcaag tgatgttcgt
    12301 tgacttaggt ggcatctgtt tagaagattc agtattgatt ggtcctcgtg tcaacctcat
    12361 cacggtgaac catctaaccg atccaaaaga acgaagaggc ttgtcagtaa agccaatcca
    12421 catcaagaaa aatgcttgga tcggagcagg agcaactatt ctgccaggcg taacgattgg
    12481 cgagaatgcc attgttgcag cgaatgcaac tgttacgaaa gatgtcccag acaataccat
    12541 cgttgcagga atccctgcca aaatcgtcaa acctgtcgaa cggattattt gaacagtaag
    12601 atgaaataag agatagtgag tataagcaag agatttttca aaacaggagg agaatagaat
    12661 gaatgattta tcagaaaaag tgattgtcat catgggcgct tcaagcggaa tcggtgaagc
    12721 aacaacgaag ctattagcag aaaaaggggc taaactagtt atagctgcac gccgagaaga
    12781 tcgcctaaaa gcaataaaag aatctttacc agaagctgag ctatatattc aaacagcaga
    12841 tgtaagagat tttgcacaag tccaagcagt tatcgattta gcgatggaaa agttcggtcg
    12901 aatcgatgta ttgtacaata atgcaggaat catgccaacc gctccattag ttgaaggtca
    12961 ccgtgatgaa tggcaaaata tgcttgatat caacatcatg ggtgtgttaa atggaatttc
    13021 cgccgtactt ccaatcatgg aaaaacaaaa atctgggcat atcatttcaa ctgattctgt
    13081 ggcgggacat gttgtttatc ctgattccgc cgtttattgc ggaacaaaat ttgctgtccg
    13141 tgcaatcatg gaaggattac gtcaagagca acggcaaaat aacatcaaat ctacgatcat
    13201 ctctccagga gctgttcaaa ctgaattata tcaaaccatc agcgacaaaa aggctgctct
    13261 tgaacttcat gaagcgcaaa aagaatgggg attgacctcc gaagatatag cttctgctgt
    13321 agcatttgcg atagaaacgc cagaccggat gtctgtcagt aacatgatta tccgtccgac
    13381 gacacaggaa gtataatcca taacggataa tatccaaaga gaattacttg ttaaatagtt
    13441 attcaaacga ccatcaattt aaggcgtgaa tggagaagaa tattcttcat ttacgtcttt
    13501 ttatgtagaa aaagcaaatt tatcaaaaag aaacaaatgt tatttaaata tggacattga
    13561 cttgctttta cttttttagt tacactgtta tttaggaaaa gtttcataat tcaaaggaga
    13621 aaaaagatga aagcaaagaa tttattattt agcgttattc ttttaggaaa tctagcagtt
    13681 gccaacacaa cagtgctagc tgaagaatcg acagaaactg tcgcaactca gacatcgagt
    13741 tcttcagaaa caggaattga aaacgataca gagagtactt tgcagtcagc tactgtctca
    13801 gaaaatacaa tttcaaccga agaaccaaca actgaaacaa cagaacaaac acaaaccgaa
    13861 gacagtacat cagcagaagc tcagaacaat ccagagaatg cagaagaaat cacgaaccaa
    13921 ttgacagatg ggcaaacatt gacacatacc catcaagcag atttgtctga attcgaaaac
    13981 tatactggct atgcttttta tgctcgctca tctgctaatt ctcaacaagc gttcattgat
    14041 agtattgcct caactgcaca aagtttggca agtgccaatg acttatatgc atcagtcatg
    14101 attgctcaag ccatcgttga aagtggctgg gggaacagta ctttggcttc tgcccctaat
    14161 tacaatctat ttgggattaa aggaagctat aatggccaat ctgttacgat gcctacttcg
    14221 gaatatgtga atgggcaatg gattacggtg aatgccgctt ttagaaaata tccttcgtac
    14281 aaagaatcat tgcaagataa cgtaacggta ttgaagacga cttcttttca gccaggcgtt
    14341 tactactatt ccggtgcatg gaaaagcaat acgaactctt acaaagatgc tacggcttgg
    14401 ctaacaggtc gttatgccac tgcgcctaat tatggttcta cattgaacaa tgtaatcgaa
    14461 acctataatc tgacacagta tgatacagca ccaacttcaa cttctccgat gtatcgtctg
    14521 tataaccgtc atacaggaga acatttatat acattgaatg ccggagaaaa aaattatctg
    14581 ccaactgtag gctgggaata tgaagggatt gcttggcaag caccaaacag cggacaacct
    14641 gtctaccgtt tatacaatcc ttacagtgga gaccatcatt acacaatggc acaatctgag
    14701 atcaactctt taacaaaaat cggttggcgt tatgaaggtc taagttttta ttctggtggt
    14761 tccaagccaa tttatcgact atttaatcca aatgaaaaaa ctggtaccca tcactatacc
    14821 ttgagtgcta aggaaagaga ttggctaaca ccgatgggct ggagatatga aggaatagga
    14881 ttttatggtt attaaaaaga ggtagtgaaa aagctgccct gcatcaagta gtaagaacag
    14941 ccaaacaaaa acagattttc atgttttttg tttggttgaa cttattaccg cagatgcaag
    15001 tttttgaaca ccgtttactc aaaaaaggtt gtgacaacat cttgtcataa cttttttatt
    15061 actgtttaag catactgatc caatttctaa tattcgacgg gattcttgca aaaggcgatt
    15121 tcggaaattt ttggcggtgt aaaattttct actgcagaag agctatcttc tatagtttga
    15181 taagaatcaa taatcttggt tttagcgttg tgctttcttt atcatttttt ccgttcattc
    15241 tacaaaaaat ttcttttctc tatacttttc tattgagagc gtttttttta ttacacagct
    15301 gattctttga cttttttctt tttttattct attattgaca agtcaatcgt tgacgagtca
    15361 attattagaa aggaggaaat tatgtcttta gaagagatta gtcacctttt atatcaaatc
    15421 aaattagccg atcaaaaaat cgtccagatc tttgaagaaa atatcggtat aagtcttact
    15481 agatacgaga tcatgatgag actgaaagaa gaaaatccat tgctacaaag cagtttgcaa
    15541 gaaaagttgc agattgatca aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat
    15601 ggctatgttt cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt agcgctaaca
    15661 gaaaaagccc agaagaaaat cgagcattgt gaaatggacc ataaagatac aggttccgtg
    15721 ctatccccgg ctttcagcga tgtagaaatg aaacaatttc taacactttt agataaattg
    15781 aatacccaac tttgcaatat aacaacagaa acgaaagagg ttaataataa tggaacaaaa
    15841 aaatgatttt tcaacaatta tgactggtag aagatccgtt cgcgtatatg atgaaacata
    15901 caaaatccca cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg caccttcttc
    15961 tgtcaatatg caaccatggc gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt
    16021 gcgtcctttg atccgtttca atacgagaca aaatgatact tcttctgcaa tggtcatgat
    16081 ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc tataaccaag cttacgaatc
    16141 agggaagatg ccaaaagaag tcagagatca acaattagct gcgatcattc cttattacaa
    16201 gagtttttca agagaagaaa tgaacgatgt agtcaaagtc gattctagct tagcggctat
    16261 gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg gtggattcga
    16321 agcagatcag ttggcagagg catttggttt ggataaagac cgttatgttc cagttattat
    16381 tttatcaata ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag caccagaaaa
    16441 gatcactacg ttcgaataaa aggttttcag ttactattca gtagctattg ctttttttga
    16501 atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta
    16561 cgtttcgaac ttttgaataa caactaaata aattcaaaag acatgtataa acaacaattt
    16621 catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga
    16681 tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt
    16741 tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg
    16801 agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc
    16861 aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc
    16921 ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt
    16981 cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat
    17041 ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa
    17101 gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt
    17161 ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa
    17221 agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt
    17281 catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa
    17341 atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag
    17401 actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc
    17461 caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa
    17521 caaatcagac cggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc
    17581 caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg
    17641 cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat
    17701 cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt
    17761 gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag
    17821 acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta
    17881 agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt
    17941 tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta
    18001 gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc
    18061 cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg
    18121 actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg
    18181 ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt
    18241 tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac
    18301 gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg
    18361 aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt
    18421 atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt
    18481 ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa
    18541 actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt
    18601 tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga
    18661 tcaattattc tctgcttata tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt
    18721 gcagttgatc atcgttggcc cgctttcaag aagtgtgcta aaagcaattc aaagtaatga
    18781 atgatggaac actgttattt gaaaaaaggc cttggcatgc tttttgtcat agtctcttga
    18841 atgcagttct agaaaaatag tgatccgaac gataggtttt ggcctgtcgt tcggatcgct
    18901 ttttcttttc caactgtatt gctcttttat tgaagataat ttgctcttca atagatattt
    18961 ctagaaaggt tctgtttatc ccttactcaa aaaactgctg catttgttct cttacttgct
    19021 gacaccattg gagttcctgc tgacaaagca taattctgcg ctggatcaca acataacgtc
    19081 caaattcttg tttcttgtct agcgagaggt cattcaattt tttttgattt ttttgcatga
    19141 ttttgttcag ctgttgttct ctagtgaata gcagtccagt agctgttgtt ttatcgagcg
    19201 tcgaaaaagc atagattttt gctagaaatt catctttttg cgtaggagcg ggagtttcct
    19261 ctttgatcca ttcctctaca agagcacgtc cttcatcagt caagtggtag atcttttttt
    19321 gagagtgctc gtctttgtcc aaaacggtca cataaccctg ctggtctaac ttggcaagcg
    19381 ttgtatagat ctgactgtga tgggcttccc aaaaaattgt gatcagctgc ttcaattcat
    19441 acccagaacg tggtttggat aaaagcacgc ataataatat ataggctaaa gtattcataa
    19501 aaatcctacc tctcgtctta ttttctttat tatatcacag taaagtttcc acgttttaca
    19561 tgtgcatatt tacatgtaaa ataaatcatg tatgatgcta atagagagaa agggatggaa
    19621 atattggaga atcaaataga tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt
    19681 tcagcgctag ttgtagcagc tacctgtatg atcgctttta tgggagtggg tctagtagac
    19741 ccaatcttaa aaacgattgc tgcacagtta aacgctacgc cagctgaaac gaccctgtta
    19801 tttactagtt acatgttagt cactggtgtg gtgatgctat ttacaggttt cttatctagt
    19861 cggatcggtt tgaaaaaaac attgatgaca ggattgttta tcatcgttgt attcgctggt
    19921 cttggcggat tatctgggaa tatcggaatg ctgattgggt tacgtgccgg ctggggtgtc
    19981 gggaatgctt tatttgtctc tactgcttta gcaacgatcg tcagtatttt agttggtaac
    20041 acagaaaaag caatcatgat gtatgaagca gcacttggct gcggaatggc tgttggccct
    20101 ctagtcggtg gggtcttggg cagtggttca tggcgttatc cattttttgg ggtagcattt
    20161 ttgatgtttt gcggttttat agccctagcg gtacttttac ctgaaactga gaaaccaaag
    20221 agaaaagtag gcctttggga aggtgtcaaa gcgttaagca atcataaact tcgttcagtt
    20281 ggactaatcg cattgttata taatttcgga ttctttactt tacttgcgta tgcgccattt
    20341 ttattgattg gctattctga attagaagtc ggttttgtat tttttggctg gggtgtgctt
    20401 ctggctattg cttcgatttt cgttgcaccg gctcttgagc ggaagtttac gacttatcgg
    20461 acaattttag cagcatttgt cttattcatt atttgtttgc tgttattagg agctggaact
    20521 tctatgccag tccttgtacc gatcagcatt gtgttagcag gatttttcca aggaattatc
    20581 aatacactgt tgacgacgat tgctatggaa ataccaggtc tgcaacgaaa cgttgcttcg
    20641 tccagctata gttttgtccg ctttttcggt ggagctttag caccatttat tgcaggtgtg
    20701 attggtgaac atattggtga aagctatgcg ttttattttg caggtgccat tgttcttatc
    20761 agtgtatgtt gtatcattta tcaccgtaat tattttgcta caaatgaaga agaataagaa
    20821 atcagaggtt gtgaaaaagc tgtcctgcat caagtcataa gaa
//
LOCUS       DAFEPQ010000213         7959 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.guided.094, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000213
VERSION     DAFEPQ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.guided.094"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            128..1564
                     /locus_tag="LP543_002918"
     CDS             128..1564
                     /locus_tag="LP543_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL2322992.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="LP543_002919"
     CDS             1753..4200
                     /locus_tag="LP543_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBL2322993.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="LP543_002920"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="LP543_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBL2322994.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="LP543_002921"
     CDS             complement(5690..6793)
                     /locus_tag="LP543_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL2322995.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="LP543_002922"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="LP543_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBL2322996.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2665 a         1359 c         1729 g         2206 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DAFEPQ010000214         6810 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.guided.102, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000214
VERSION     DAFEPQ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.guided.102"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..478
                     /locus_tag="LP543_002923"
     CDS             <1..478
                     /locus_tag="LP543_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL2322997.1"
                     /translation="RKVKTLTLIYKGENTKTVHSAYIVSVYVDSTGNMVLIKNPTITS
                     IPKKSDYKPKAIESEGTVDSITTNEINEFLTTFFKLYPTATASELSYYVNDGILKPIG
                     KEYIFQELVNPIYNRKDNQVTVSLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            723..839
                     /locus_tag="LP543_002924"
     CDS             723..839
                     /locus_tag="LP543_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBL2322998.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            855..2774
                     /gene="tet(M)"
                     /locus_tag="LP543_002925"
     CDS             855..2774
                     /gene="tet(M)"
                     /locus_tag="LP543_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBL2322999.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2872..4245
                     /gene="tet(L)"
                     /locus_tag="LP543_002926"
     CDS             2872..4245
                     /gene="tet(L)"
                     /locus_tag="LP543_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBL2323000.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            4809..6071
                     /locus_tag="LP543_002927"
     CDS             4809..6071
                     /locus_tag="LP543_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBL2323001.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            6316..6777
                     /locus_tag="LP543_002928"
                     /pseudo
     CDS             6316..6777
                     /locus_tag="LP543_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
BASE COUNT         2331 a          995 c         1396 g         2088 t
ORIGIN      
        1 ccggaaggtt aaaaccctaa ctctaatata caagggggaa aatacaaaga ccgtccactc
       61 tgcttatata gtgagtgtct atgtagacag tactggaaat atggtcctca ttaagaatcc
      121 gaccattact agcataccaa agaaatcaga ctataaacca aaagctattg aaagtgaggg
      181 tacggttgat tccattacaa ccaatgaaat caatgagttt ttaacgacgt tcttcaagct
      241 ctatcctaca gcgacagcca gtgaactttc ctactatgtg aatgacggga tattaaaacc
      301 aatcggaaaa gagtacatct ttcaagaact ggtaaatcct atttacaatc gtaaggataa
      361 tcaagtcacg gtatcgctga cagtggagta tatcgaccag cagaccaaag caacgcaggt
      421 atctcaattt gatttggtac ttgaaaagaa cgggagtaat tggaagatta tagaataaca
      481 aatattggta cattattaca gctattttgt aatcacgtac tctctttgat aaaaaattgg
      541 agattccttt acaaatatgc tcttacgtgc tattatttaa gtatctattt aaaaggagtt
      601 aataaatatg cggcaaggta ttcttaaata aactgtcaat ttgatagtgg gaacaaataa
      661 ttggatgtcc ttttttagga gggcttagtt ttttgcaccc agtttaagaa tacctttatc
      721 atgtgattct aaagtatccg gagaatatct gtatgctttg tatgcctatg gttatgcata
      781 aaaatcccag tgataagagt atttatcact gggattttta tgcccttttg ggcttttgaa
      841 tggaggaaaa tcacatgaaa attattaata ttggagtttt agctcatgtt gatgcgggaa
      901 aaactacctt aacagaaagc ttattatata acagtggagc gattacagaa ttaggaagcg
      961 tggacaaagg tacaacgagg acggataata cgcttttaga acgtcagaga ggaattacaa
     1021 ttcagacagg aataacctct tttcagtggg aaaatacgaa ggtgaacatc atagacacgc
     1081 caggacatat ggatttctta gcagaagtat atcgttcatt atcagtttta gatggggcaa
     1141 ttctactgat ttctgcaaaa gatggcgtac aagcacaaac tcgtatatta tttcatgcac
     1201 ttaggaaaat ggggattccc acaatctttt ttatcaataa gattgaccaa aatggaattg
     1261 atttatcaac ggtttatcag gatattaaag agaaactttc tgccgaaatt gtaatcaaac
     1321 agaaggtaga actgtatcct aatatgtgtg tgacgaactt taccgaatct gaacaatggg
     1381 atacggtaat agagggaaac gatgaccttt tagagaaata tatgtccggt aaatcattag
     1441 aagcattgga actcgaacaa gaggaaagca taagatttca gaattgctcc ttgtaccctg
     1501 tttatcatgg aagcgcaaaa agcaacatag ggattgagca gcttatagaa gtgataacga
     1561 ataaatttta ttcatcaaca tacagaaaga agtctgaact ttgcggaaat gtcttcaaaa
     1621 ttgaatattc ggaagaaaga caacgtcttg catatgtacg cctttatggc ggaatcctgc
     1681 atttgcggga ttcggttaga atatcggaaa aggaaaaaat aaaaattaca gaaatgtata
     1741 cttcaataaa tggtgaatta tgtaaaattg ataaggctta ttccggggaa attgttattt
     1801 tgcaaaatga gtttttgaag ctaaatagtg ttcttggaga tacaaagcta ttgccacaga
     1861 gagagagaat tgaaaatccg ctccctctgc tgcaaacaac tgttgaaccg agcaaacctc
     1921 aacaaaggga aatgttactt gatgcacttt tagaaatctc cgacagtgac ccgcttctac
     1981 aatattatgt ggattctacg acacatgaaa ttatactttc tttcttaggg aaagtacaaa
     2041 tggaagtgat tagtgcactg ttgcaagaaa agtatcatgt ggagatagaa ctaaaagagc
     2101 ctacagtcat ttatatggag agaccgttaa aaaatgcaga atataccatt cacatcgaag
     2161 tgccgccaaa tcctttctgg gcttccattg gtttatctgt atcaccgctt ccgttgggaa
     2221 gtggaatgca gtatgagagc tcggtttctc ttggatactt aaatcaatca tttcaaaatg
     2281 cagttatgga agggatacgc tatggttgcg aacaaggatt atatggttgg aatgtgacgg
     2341 attgtaaaat ctgttttaag tatggcttat actatagccc tgttagtacc ccagcagatt
     2401 ttcggatgct tgctcctatt gtattggaac aagtcttaaa aaaagctgga acagaattgt
     2461 tagagccata tcttagtttt aaaatttatg cgccacagga atatctttca cgagcataca
     2521 acgatgctcc taaatattgt gcgaacatcg tagacactca attgaaaaat aatgaggtca
     2581 ttcttagtgg agaaatccct gctcggtgta ttcaagaata tcgtagtgat ttaactttct
     2641 ttacaaatgg acgtagtgtt tgtttaacag agttaaaagg gtaccatgtt actaccggtg
     2701 aacctgtttg ccagccccgt cgtccaaata gtcggataga taaagtacga tatatgttca
     2761 ataaaataac ttagtgtatt ttatgttgtt atataaatat ggtttcttgt taaataagat
     2821 gaaatatttt ttaataaaga tttgaattaa agtgtaaagg aggaattaat tatgaagtgt
     2881 aatgaatgta acagggttaa atacaaccaa attttaattt ggctttgcgt tttatctttt
     2941 tttagcgtat taaatgaaat ggttttgaac gtctcattac ctgatattgc aaatgatttt
     3001 aataaaccac ctgcgagtac aaactgggtg aacacagcct ttatgttaac cttttccatt
     3061 ggaacagctg tatatggaaa gctatctgat caattaggca tcaaaaggtt actcctattt
     3121 ggaattataa taaattgttt cgggtcggta attgggtttg ttggccattc tttcttttcc
     3181 ttacttatta tggctcgttt tattcaaggg gctggtgcag ctgcatttcc agcactcgta
     3241 atggttgtag ttgcgcgcta tattccaaag gaaaataggg gtaaagcatt tggtcttatt
     3301 ggatcgatag tagccatggg agaaggagtc ggtccagcga ttggtggaat gatagcccat
     3361 tatattcatt ggtcctatct tctactcatt cctatgataa caattatcac tgttccgttt
     3421 cttatgaaat tattaaagaa agaagtaagg ataaaaggtc attttgatat caaaggaatt
     3481 atactaatgt ctgtaggcat tgtatttttt atgttgttta caacatcata tagcatttct
     3541 tttcttatcg ttagcgtgct gtcattcctg atatttgtaa aacatatcag gaaagtaaca
     3601 gatccttttg ttgatcccgg attagggaaa aatatacctt ttatgattgg agttctttgt
     3661 gggggaatta tatttggaac agtagcaggg tttgtctcta tggttcctta tatgatgaaa
     3721 gatgttcacc agctaagtac tgccgaaatc ggaagtgtaa ttattttccc tggaacaatg
     3781 agtgtcatta ttttcggcta cattggtggg atacttgttg atagaagagg tcctttatac
     3841 gtgttaaaca tcggagttac atttctttct gttagctttt taactgcttc ctttctttta
     3901 gaaacaacat catggttcat gacaattata atcgtatttg ttttaggtgg gctttcgttc
     3961 accaaaacag ttatatcaac aattgtttca agtagcttga aacagcagga agctggtgct
     4021 ggaatgagtt tgcttaactt taccagcttt ttatcagagg gaacaggtat tgcaattgta
     4081 ggtggtttat tatccatacc cttacttgat caaaggttgt tacctatgga agttgatcag
     4141 tcaacttatc tgtatagtaa tttgttatta cttttttcag gaatcattgt cattagttgg
     4201 ctggttacct tgaatgtata taaacattct caaagggatt tctaaatcgt taagggatca
     4261 actttgggag agagttcaaa attgatcctt tttttataac aggaattcaa atctttttgt
     4321 tccattaaag ggcgcgattg ctgaataaaa gatacgagag acctctcttg tatctttttt
     4381 attttgagtg gttttgtccg ttacactaga aaaccgaaag acaataaaaa ttttattctt
     4441 gctgagtctg gctttcggta agctagacaa aacggacaaa ataaaaattg gcaagggttt
     4501 aaaggtggag attttttgag tgatcttctc aaaaaatact acctgtccct tgctgatttt
     4561 taaacgagca cgagagcaaa accccccttt gctgaggtgg cagagggcag gtttttttgt
     4621 ttcttttttc tcgtaaaaaa aagaaaggtc ttaaaggttt tatggttttg gtcggcactg
     4681 ccgacagcct cgcagagcac acactttatg aatataaagt atagtgtgtt atactttact
     4741 tggaagtggt tgccggaaag agcgaaaatg cctcacattt gtgccaccta aaaaggagcg
     4801 atttacatat gagttatgca gtttgtagaa tgcaaaaagt gaaatcagct ggactaaaag
     4861 gcatgcaatt tcataatcaa agagagcgaa aaagtagaac gaatgatgat attgaccatg
     4921 agcgaacacg tgaaaattat gatttgaaaa atgataaaaa tattgattac aacgaacgtg
     4981 tcaaagaaat tattgaatca caaaaaacag gtacaagaaa aacgaggaaa gatgctgttc
     5041 ttgtaaatga gttgctagta acatctgacc gagatttttt tgagcaactg gatccaggag
     5101 aacaaaaacg attttttgag gaaagttata aattattttc cgaacgatat ggcaagcaaa
     5161 atattgctta tgcaacagtt cataatgatg agcaaacccc tcacatgcat ttaggtgttg
     5221 tgcctatgcg tgatggaaaa ctgcaaggaa aaaatgtgtt taatcgtcaa gaactgttat
     5281 ggctacaaga taaattcccc gagcatatga aaaaacaggg ttttgagttg aagcgtggtg
     5341 aacgtggctc tgaccgtaaa catattgaga cagctaaatt taaaaaacaa actttggaaa
     5401 aagagattga ttttctagaa aaaaatttag cagttaaaaa agatgaatgg actgcttata
     5461 gcgataaagt taaatcagat ttagaagtac cagcgaaacg acacatgaaa agtgttgaag
     5521 tgccaacggg tgaaaagtcc atgtttggtt tgggaaaaga aataatgaaa acagaaaaga
     5581 aaccaaccaa aaatgttgtt atatcggagc gtgattataa aaacttagtg actgctgcga
     5641 gagataacga taggttaaaa cagcatgtta gaaatctcat gagtactgat atggcgagag
     5701 aatataaaaa attaagtaaa gaacatgggc aagttaaaga aaaatatagt ggtcttgtag
     5761 agcgatttaa tgaaaatgta aatgattata atgagttgct tgaagaaaac aagtctttaa
     5821 agtctaaaat aagcgattta aagcgtgatg tgagtttaat ctatgaaagc actaaggaat
     5881 tccttaagga acgtacagac ggcttaaaag cctttaaaaa cgtttttaag gggtttgtag
     5941 acaaggtaaa ggataaaaca gcacaattcc aagaaaaaca cgatttagaa cctaaaaaga
     6001 acgaatttga actaactcat aaccgagagg taaaaaaaga acgaagtcga gatcagggaa
     6061 tgagtttata aaataaaaaa agcacctgaa aaggtgtctt tttttgatgg ttttgaactt
     6121 gttctttctt atcttgatat atatagaaat aacgtcattt ttattttagt tgctgaaagg
     6181 tgcgttgaag tgttggtatg tatgtgtttt aaagtattga aaacccttaa aattggttgc
     6241 acagaaaaac cccatctgtt aaagttataa gtgaccaaac aaataactaa atagatgggg
     6301 gtttctttta atattatgtg tcctaatagt agcatttatt cagatgaaaa atcaagggtt
     6361 ttagtggaca agacaaaaag tggaaaagtg agaccatgga gagaaaagaa aatcgctaat
     6421 gttgattact ttgaacttct gcatattctt gaatttaaaa aggctgaaag agtaaaagat
     6481 tgtgctgaaa tattagagta taaacaaaat cgtgaaacag gcgaaagaaa gttgtatcga
     6541 gtgtggtttt gtaaatccag gctttgtcca atgtgcaact ggaggagagc aatgaaacat
     6601 ggcattcagt cacaaaaggt tgttgctgaa gttattaaac aaaagccaac agttcgttgg
     6661 ttgtttctca cattaacagt taaaaatgtt tatgatggcg aagaattaaa taagagtttg
     6721 tcagatatgg ctcaaggatt tcgccgaatg atgcaatata aaaaaattaa taaaaatggt
     6781 tctgttgcaa agttttaaat aaagaataaa
//
LOCUS       DAFEPQ010000215         5112 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.guided.114, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000215
VERSION     DAFEPQ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.guided.114"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            35..280
                     /locus_tag="LP543_002929"
     CDS             35..280
                     /locus_tag="LP543_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL2323002.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            383..1252
                     /locus_tag="LP543_002930"
     CDS             383..1252
                     /locus_tag="LP543_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBL2323003.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1233..1967
                     /locus_tag="LP543_002931"
     CDS             1233..1967
                     /locus_tag="LP543_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL2323004.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2000..2912
                     /gene="ant(6)"
                     /locus_tag="LP543_002932"
                     /pseudo
     CDS             2000..2912
                     /gene="ant(6)"
                     /locus_tag="LP543_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            2909..3451
                     /gene="sat4"
                     /locus_tag="LP543_002933"
     CDS             2909..3451
                     /gene="sat4"
                     /locus_tag="LP543_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HBL2323005.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            3544..4338
                     /locus_tag="LP543_002934"
                     /note="aph(3')-IIIa"
     CDS             3544..4338
                     /locus_tag="LP543_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBL2323006.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            4614..5102
                     /locus_tag="LP543_002935"
     CDS             4614..5102
                     /locus_tag="LP543_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBL2323007.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISVLLQS
                     FK"
BASE COUNT         1760 a          746 c         1157 g         1449 t
ORIGIN      
        1 ggggctgacc cattattaat aaggagtgga caacatgatt gatgtaaatg agctattgga
       61 agaagctatt agagaaactg aaaatctaaa tgatggcgaa gtttttcttg ttaaagattt
      121 attcaaggga tatgtgggga ataggatacc cagaaaggat cgacttctgc ttggaacgtt
      181 atttttaaac catgttagca aaatgaatgg taatttaaag gctattgaaa agacttcatc
      241 taatcagcag aggtataaaa agacaattga taagttttaa ctatcttttg tgaaattaaa
      301 aaaattataa atatgttgag tagaagcaaa agatttggct tggaattttt acagcagtga
      361 atcgataaaa ggagcatgct tgatggtaga taatattatt aaatcagtag cagagaaatt
      421 atcctctctg tcttatatag aaggtattgt tttaggtggt tcacgtgcaa ggggcaccca
      481 tacagaggat tcggatatag atatcggcat ctattacaat tcagaatcat ttgacataaa
      541 tactattaat caattcgcta caaagctgga tgatgagcat agaaataacc ttgttgtacc
      601 tcccggagca tggggtgatt ggattaatgg cggcggatgg ttagtcataa acgggtatca
      661 tgtggattta attttacgtg atattaaacg tgtggaacaa ataatgaaag atacagagca
      721 cggaattgtt actgccaatt atcagactgg gcatccccat ggttatatta gtgcaatgta
      781 tcgaggagaa ttagcgatta gcaaaatact atatgctaag aatgaaagct tatgcgaatt
      841 aaaaaaacag gcagaaactt atcccaatgc tttgcagaaa agtttagtta acttttttat
      901 gtttgaagca gggttctctt taatgtttgt aaaagcaaat tcgggaacag acgataaata
      961 ttatattgcg ggtcatgttt ttcgtatagt ttcatgttta aatcaagtgt tatttgcatg
     1021 taataatgct tattgtatca acgaaaagaa agctataaaa ctgcttgaaa cttttgaaca
     1081 taaacctgaa aaatataccg agaaggtaaa tcatattttt gaagtactcg gtatctcact
     1141 ttttgaatgc tacgacatga ccgagaagct ttataatgaa gtgaatgaaa ttgtatcgga
     1201 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1261 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1321 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1381 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1441 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1501 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1561 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1621 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1681 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1741 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1801 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1861 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1921 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1981 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     2041 agcagaacag gatgaacgaa cgtattcgaa ttgtgaccct tgaggggtca cgcgcaaata
     2101 ttaatatacc taaagatgaa tttcaggatt atgatattac atattttgta agtgatatag
     2161 aaccgtttat atctaatgat gactggctta atcaatttgg gaatataata atgatgcaaa
     2221 agccggagga tatggaatta ttcccacctg aagaaaaggg attttcctat cttatgctat
     2281 ttgatgatta caataaaatt gatcttacct tattgccctt ggaagagtta gataattacc
     2341 taaagggcga taaattaata aaggttctaa ttgataaaga ttgtagaatt aaaagggaca
     2401 tagttccgac tgatatagat tatcatgtaa gaaagccaag cgcaagggag tatgatgatt
     2461 gctgcaatga attttggaat gtaacacctt atgttattaa aggattgtgc cgtaaggaaa
     2521 ttttatttgc tattgatcat tttaatcaga ttgttcgcca tgagctgctg agaatgatat
     2581 catggaaggt cggcatcgaa acaggcttta aattaagtgt aggcaagaac tataagttta
     2641 ttgaaaggta tatatccgag gatttgtggg agaaactttt gtccacctac cggatggatt
     2701 cctatgaaaa catatgggaa gcattatttc tatgccatca attgttcagg gcggtatccg
     2761 gtgaggtggc ggaaaggctt cattatgcct atccggagta tgataggaat ataacaaaat
     2821 ataccaggga catgtataaa aaatacactg gtaaaaccgg ctgcctggat agcacatatg
     2881 ccgctgatat agaagagagg cgggaacagt gattacagaa atgaaagcag ggcacctgaa
     2941 agatatcgat aaacccagcg aaccatttga ggtgataggt aagattatac cgaggtatga
     3001 aaacgagaat tggaccttta cagaattact ctatgaagcg ccatatttaa aaagctacca
     3061 agacgaagag gatgaagagg atgaggaggc agattgcctt gaatatattg acaatactga
     3121 taagataata tatctttact accaagacga taaatgcgtc ggaaaagtta aactgcgaaa
     3181 aaattggaac cggtacgctt atatagaaga tatcgccgta tgtaaggatt tcagggggca
     3241 aggcataggc agcgcgctta tcaatatatc tatagaatgg gcaaagcata aaaacttgca
     3301 tggactaatg cttgaaaccc aggacaataa ccttatagct tgtaaattct atcataattg
     3361 tggtttcaaa atcggctccg tcgatactat gttatacgcc aactttgaaa acaactttga
     3421 aaaagctgtt ttctggtatt taaggtttta gaatgcaagg aacagtgaat tggagttcgt
     3481 cttgttataa ttagcttctt ggggtatctt taaatactgt agaaaagagg aaggaaataa
     3541 taaatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc
     3601 gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg agaaaatgaa
     3661 aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga tgtggaacgg
     3721 gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt cctgcacttt
     3781 gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt cctttgctcg
     3841 gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc ggagtgcatc
     3901 aggctctttc actccatcga catatcggat tgtccctata cgaatagctt agacagccgc
     3961 ttagccgaat tggattactt actgaataac gatctggccg atgtggattg cgaaaactgg
     4021 gaagaagaca ctccatttaa agatccgcgc gagctgtatg attttttaaa gacggaaaag
     4081 cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat ctttgtgaaa
     4141 gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga caagtggtat
     4201 gacattgcct tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca gtatgtcgag
     4261 ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata ttatatttta
     4321 ctggatgaat tgttttagta cctagattta gatgtctaaa aagctttaac tacaagcttt
     4381 ttagacatct aatcttttct gaagtacatc cgcaactgtc catactctga tgttttatat
     4441 cttttctaaa agttcgctag atagagttct atattagaga tgtaaagagt tttggtgaag
     4501 aggtttgtga gtatggcttt tataaccagg gtgcaatgag aagcactaac agcagctagc
     4561 ttaagaacgc ataggatata ctttttatgg aagtcacaga ggaggaataa aatttgaaca
     4621 ggattaacag agtaacctct attcttattc agctgcaatc aaaaaaaata attcctgcca
     4681 aagaaattgc acagcgattt aatataagct taaggacagt ttacagagat atccggacac
     4741 ttgaagaagc cggaatacca attggatctg aggccggaaa aggatatttt cttgtagagg
     4801 gcttcctact tccgccggta atgtttacag cggcggaagt gggtgcattg attacagcag
     4861 ggaaattttt aaattgccat ggagatgaat catttataaa ggattttgat tcagctatgt
     4921 ataaaatcaa atctattttg aagcatggtg aaaaaaacta tgcacaggag ttggagaaca
     4981 gtattaacgt gtacagcaca tctggacaga aaaatacatt ggcggataac gttattgcag
     5041 caattcagac tgcaatctgc aataaaaggg taatatcggt tctgttgcaa agttttaaat
     5101 aaagaataaa at
//
LOCUS       DAFEPQ010000216         4864 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.guided.117, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000216
VERSION     DAFEPQ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.guided.117"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            363..1274
                     /locus_tag="LP543_002936"
     CDS             363..1274
                     /locus_tag="LP543_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HBL2323008.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1427..1912
                     /gene="vanZ"
                     /locus_tag="LP543_002937"
     CDS             1427..1912
                     /gene="vanZ"
                     /locus_tag="LP543_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBL2323009.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(2199..2528)
                     /locus_tag="LP543_002938"
     CDS             complement(2199..2528)
                     /locus_tag="LP543_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2323010.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2833..3162
                     /locus_tag="LP543_002939"
     CDS             2833..3162
                     /locus_tag="LP543_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL2323011.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            3152..3439
                     /locus_tag="LP543_002940"
     CDS             3152..3439
                     /locus_tag="LP543_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL2323012.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(3718..4281)
                     /locus_tag="LP543_002941"
     CDS             complement(3718..4281)
                     /locus_tag="LP543_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2323013.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         1705 a          734 c          837 g         1588 t
ORIGIN      
        1 tatttgatag tagatttaaa actttgcaac agaaccattg aagatgtaag gcagagcgat
       61 attgcggtca ttatctgcgt gcgctgcggc aagatagcct gataataaga ctgatcgcat
      121 agaggggtgg tatttcacac cgcccattgt caacaggcag ttcagcctcg ttaaattcag
      181 catgggtatc acttatgaaa attcatctac attggtgata atagtaaatc cagtagggcg
      241 aaataattga ctgtaattta cggggcaaaa cggcacaatc tcaaacgaga ttgtgccgtt
      301 taaggggaag attctagaaa tatttcatac ttccaactat atagttaagg aggagactga
      361 aaatgaagaa gttgtttttt ttattgttat tgttattctt aatatactta ggttatgact
      421 acgttaatga agcactgttt tctcaggaaa aagtcgaatt tcaaaattat gatcaaaatc
      481 ccaaagaaca tttagaaaat agtgggactt ctgaaaatac ccaagagaaa acaattacag
      541 aagaacaggt ttatcaagga aatctgctat taatcaatag taaatatcct gttcgccaag
      601 aaagtgtgaa gtcagatatc gtgaatttat ctaaacatga cgaattaata aatggatacg
      661 ggttgcttga tagtaatatt tatatgtcaa aagaaatagc acaaaaattt tcagagatgg
      721 tcaatgatgc tgtaaagggt ggcgttagtc attttattat taatagtggc tatcgagact
      781 ttgatgagca aagtgtgctt taccaagaaa tgggggctga gtatgcctta ccagcaggtt
      841 atagtgagca taattcaggt ttatcactag atgtaggatc aagcttgacg aaaatggaac
      901 gagcccctga aggaaagtgg atagaagaaa atgcttggaa atacgggttc attttacgtt
      961 atccagagga caaaacagag ttaacaggaa ttcaatatga accatggcat attcgctatg
     1021 ttggtttacc acatagtgcg attatgaaag aaaagaattt cgttctcgag gaatatatgg
     1081 attacctaaa agaagaaaaa accatttctg ttagtgtaaa tggggaaaaa tatgagatct
     1141 tttattatcc tgttactaaa aataccacca ttcatgtgcc gactaatctt cgttatgaga
     1201 tatcaggaaa caatatagac ggtgtaattg tgacagtgtt tcccggatca acacatacta
     1261 attcaaggag gtaaggatgg cggaatgaaa ccaacgaaat taatgaacag cattattgta
     1321 ctagcacttt tggggtaacg ttagcttttt aatttaaaac ccacgttaac taggacattg
     1381 ctatactaat gatacaactt aaacaaaaga attagaggaa attatattgg gaaaaatatt
     1441 atctagagga ttgctagctt tatatttagt gacactaatc tggttagtgt tattcaaatt
     1501 acaatacaat attttatcag tatttaatta tcatcaaaga agtcttaact tgactccatt
     1561 tactgctact gggaatttca gagagatgat agataatgtt ataatcttta ttccatttgg
     1621 cttgcttttg aatgtcaatt ttaaagaaat cggattttta cctaagtttg cttttgtact
     1681 ggttttaagt cttacttttg aaataattca atttatcttc gctattggag cgacagacat
     1741 aacagatgta attacaaata ctgttggagg ctttcttgga ctgaaattat atggtttaag
     1801 caataagcat atgaatcaaa aaaaattaga cagagttatt atttttgtag gtatactttt
     1861 gctcgtatta ttgctcgttt accgtaccca tttaagaata aattacgtgt aagatgtcta
     1921 aatcaagcaa tctgatcttt catacacata aagatattga atgaattgga ttagatggaa
     1981 aacgggatgt ggggaaactc gcccgtaggt gtgaagtgag gggaaaaccg gtgataaagt
     2041 aaaaagctta cctaacacta tagtaacaaa gaaagcccaa ttatcaattt tagtgctgag
     2101 gaattggtct ctttaataaa tttccttaac gttgtaaatc cgcattttcc tgacggtacc
     2161 cccaaactaa gatttcatag atagattttc tgataacttt aaaaaacacg aatagcggaa
     2221 aggatgtctt cattcgctaa agagtaataa actacttttc catcttgacg tgattttgcc
     2281 attccatgtt tttttaaaaa acgtaaatgg tgtgacgttg tagcgacact agcagtaagt
     2341 atttctgcta aatcacagac gcacatttcc ttataggttt ctaaagcata gaaaatttta
     2401 attctagaaa aatcgctgaa acatttccct aacacaagta agtttgaaaa atctttttga
     2461 tccaatttat tttttattcc atttactttt tctttatgta cgacagtcac ttggcaaact
     2521 tttttcattc atctcacctc acattcaaac gattatttga atgaagtata cctatttgat
     2581 cactcttttg tcaatctacc ttaattccaa tacactatta taagcaattt catgtattga
     2641 agtaaactta cttgcaagtg cgtttttgat tccaagttaa aaacatatta ttattctaca
     2701 taatttccta atcaaataaa actcctttga aaattgttct aaaggagttt tattattttg
     2761 tatttctact aaaaatagcg tacaatgtaa ttactaagta attacgaaat gtaaaataat
     2821 ggaggaaata atatgattac aaaaacaaga aaacaaggaa attctattat gttaacggtc
     2881 cctaaagaat tcgatgtgcc caatggtgtt gaagtagaag ctaagcttgt agagaatggt
     2941 atcctttatg agtttgtaga gcctaaaaaa gaattttttg attttagtga agatattttg
     3001 gctgatattc tttcagaagg ttatagcaaa caagagatct taaaagaatt tagaaataga
     3061 aaaaatgcgt taacttctgc atttaaatct attgctgaag atactgttgc aaattctaaa
     3121 ccgatgacaa aagaggagtt ggcagcagaa attggcttat gagattctac cttctaaaca
     3181 cgtaataaag tatctaaaaa aattaaaaga gaaaacatta aaagaacaat ttttaacgat
     3241 aatttatgat gagatagcag ttcgtcctca ttcgggagaa caaaaaacgg gtgatttatc
     3301 aggaatttgg gctattggat tcaagtatgc aggtacgaca tatcgcgttg catatgaaat
     3361 taaagataat gcagtgatac ctatcttact atgtggaacg catgaaaatt tttatgaaca
     3421 attaaaaaag attaggtaag aacccaatgt ttcaacttga gaggatagac gacactttaa
     3481 aagataattt ttaacagctg agagttgtaa taccctataa ttttaaacca ctaacctata
     3541 tatttatgaa aaatagaaaa aattaaaacg cattttaagc ctctttcaaa ttaaaagctt
     3601 catccttatg cacaaagagg gcttaaaatg cgttatttta tattgttttt tagtaataat
     3661 aaaaaaatca ctctaattct cccataaata ttatagattc ctaaagatta aaatagatta
     3721 ttccctattg tcttttaagt agcggtagag tgttgccttg ctaataccag tcatttccgt
     3781 aatttctttt acgctataaa cctgactttt atacaatttt actgctcgat caacatcttt
     3841 ctggttcact cgtggacggc cacctttacg acctctcgca cgtgcacttt tcaaaccttc
     3901 tttggtacgt tcaacaatta aatcacgttc gaactgacta aatgcttgga agacagtcat
     3961 cattaagcgt ccgtgaggcg tgctggtatc gaagttttct ttcaagctca ccagcttaac
     4021 atggtgttct tcaaaatagg taacaaggtc aatcaaatcc tttgtactac gacccaatcg
     4081 agaaaaactt tctacaatta ccgtatctcc cggacgaatt ttatctttta aacgatttag
     4141 ttggggacga accgctttgg ttcccgtcat tttctcagtt aggatttcat tacaattttg
     4201 ttcattgagt aagtctaatt gacgatttag ttcttgtgaa cgtgtactca ctcgggcata
     4261 accataaatg tatccactca tgcttttctt cctttcgtac tttaattctg gcttgattgt
     4321 atcataaaag ggagttatag atacatagtt aatgcaacaa gttatgatac aaaatatgac
     4381 ctcttttatt taataaaaga ggtcatttca ttttgtctca taatcgattg tttttgatac
     4441 gagtgtgtat acgaaataaa caaaaaaatc ttaagtaaat ttctagatac ctaatttaat
     4501 cctaactagt gtatcctaaa gttaataacg agtttatcac aatgaaaatt ataaaatgct
     4561 cccatattag atataacatg caatatttga attagaacta caaaatttat tcgttttata
     4621 aagtaaataa tgttgagata ctaaggaata gaatattata aggaattgag ctgaatgtat
     4681 tggataaaaa aagaataact ttattagaga tggactataa aaatgctcgt aattttcttc
     4741 tgaagtctga aagctatttg aatgctcagc taccagaata tattagtttt gacgaaactt
     4801 taaaatctgc taaaaaagtt ttaacttcgc cgtttgtata attaagcgag acaaataaaa
     4861 aagc
//
LOCUS       DAFEPQ010000217         4404 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.guided.125, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000217
VERSION     DAFEPQ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.guided.125"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            202..1416
                     /locus_tag="LP543_002942"
     CDS             202..1416
                     /locus_tag="LP543_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBL2323014.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFRLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1447..1665
                     /locus_tag="LP543_002943"
                     /pseudo
     CDS             1447..1665
                     /locus_tag="LP543_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2084..2557
                     /gene="dfrF"
                     /locus_tag="LP543_002944"
     CDS             2084..2557
                     /gene="dfrF"
                     /locus_tag="LP543_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBL2323015.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            2684..3340
                     /locus_tag="LP543_002945"
     CDS             2684..3340
                     /locus_tag="LP543_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBL2323016.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            3522..4052
                     /locus_tag="LP543_002946"
     CDS             3522..4052
                     /locus_tag="LP543_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL2323017.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
BASE COUNT         1462 a          654 c          938 g         1350 t
ORIGIN      
        1 tcaattctga ttcatcagtc ccataaatta tagagcctat tttttgtctt tatttgtgtg
       61 aaaaacgaaa aagagcacaa cacatcaagc ttttattaat tgtctgtctt ttataaatgc
      121 aagtattaat tcaaaaaaac agaagaaaaa aacaactaca ccttctttta tactcgtaat
      181 cgtagaaagg aggcgaaaaa aatgaataca ttacctatca atacaggaga tacagcattc
      241 atgattttat gtacagcaat ggtttgcctg atgactcctg gtctagcttt tttttacggc
      301 ggattagcac ggaagaataa tatactaacg atcatgtctc aatcttttct ctccgttggg
      361 ataacaacga ttatgtgggt atttggtgga ttcagattgg cctttggacg ggatatcggc
      421 ggtgttattg gaaacccggc agatttcttt ttgatgagac atgttacaag cttccccaac
      481 atgtttcatg gtgcaacgat tccattttta atgttctttc tttaccaact gatgtttatc
      541 gtgattaccg ttcctttgat gacaggagct tttgcaggga gattgaattt aaaaggctat
      601 attatacttg ttattttttg gaatctgctg atttatttcc ctgtttgcca ctggatatgg
      661 ggtggcggat ttttggatca gatgggattt agagatttcg ctggtggagc agtcatccac
      721 acgacagctg gattcggctc tttagcatgt atcctcacac ttggtcaaag aaagattcct
      781 ttaggagaaa gaagtcagca tagcagtctt atggcagctg ctatcggtac aggactgctt
      841 tggtttggct ggttcggttt taattccggt ggtgcacttc gagcagatga acaagcagtc
      901 aatgcatttg gttcaacatt catcgctctt gcatttgcga tgattacttg gctgatcatt
      961 gcaaaagtaa aaggaaatgg ctttgatttt gtcgacgtac taacaggatc cgttgctgga
     1021 ttggcagcca ttacgccttg tgccggttat gtagaagcaa aaagtgccat gctgattggg
     1081 atcattgcgg gtatcgtctg tcatgctgct gttgattttc gtaaaaagaa acaatgggat
     1141 gatgcacttg atgtgtgggg tgttcatggc atgggcggat tcacaggtac gattctgata
     1201 gggatctttg catctaacag ccatttacta acgaatccaa gcagctggta cttcttaggg
     1261 attcaagtga ttggtgtcat cattactgcc gtgtatgcct atgtgctgac aacagtgatt
     1321 ttaaaatcgg cgatgcactt tacaacgatc actactacaa aagaagaaca agaagaagga
     1381 ttggactttt tcttctttgg caacaaactc aattgaaaca aattcaaata gaaaagagga
     1441 agattcatgc aacaaagcaa aacgaaaatt cctgcaaaaa aattaacgtt ctttggcttt
     1501 ttggctatga ccatctcaat ggtcgtttca ttatatgaat atcccacatt cgcgacctca
     1561 ggcttcagct tagtattttt cttgttatta ggcggtttgc tatggtttat ccctgtggca
     1621 ttgtgcgcag cagagatggc aacggtgaaa ggatgggaaa aaggtgtttc ccaaacaaaa
     1681 agatattaag aaacagccca tgtttacctg cttgcaaaca aaagaatatt gctactagac
     1741 taagagatac tacataagtg tgttggtatc tcttttttgt tgccgtgaaa cagcagacta
     1801 tttcatttct gcttgattac cttaaaactt attagtctat ggtttgtcaa gggcttgttg
     1861 cgtgagaaat gctgaatatc accctttaca aaacataggc tgataagtaa cttttcaaaa
     1921 gcagaaaaga aatggcaatg cttataacaa tatgatataa tgcagatgaa gacaaaaaag
     1981 gaatttgtcg agctggttag ttcgagataa ttttgagttt gttaagatgt ggtgcacttg
     2041 atggaatcac aggtacgaca tcatattatg gaggatgaaa tagatgatag gtttgattgt
     2101 tgcgaggtca aagaataatg ttataggcaa gaatggtaat ataccatgga aaataaaggg
     2161 agaacaaaag caatttagag agttaacaac gggtaatgtg gttattatgg ggcgaaagtc
     2221 ttatgaagaa atcggtcatc cgttgcctaa tagaatgaat attgttgttt ccaccacaac
     2281 agagtatcaa ggagataatt tagtttcagt taaatcatta gaagatgcat tattattggc
     2341 taaaggacga gatgtataca tatctggtgg atatggacta tttaaggaag ctttgcaaat
     2401 agtagataaa atgtatatca cagaagtaga tttaaatatt gaagatggag atacattctt
     2461 tccagaattt gatatcaatg attttgaagt tttgataggg gaaacacttg gtgaggaagt
     2521 gaaatatacg agaacatttt atgtaaggaa aaaatgaatt gagtaaattt tggatttagg
     2581 gtgttttcat aaatatattg ctttttatgc atatataaat tgttgtaaga caaattccag
     2641 tttgtatggg gcgataaaat cagaatttga gagaggattg aatatgaata aggaatggtc
     2701 tgaacttaat aaaacaatgc aggcacaaat aaaaaagaaa gatacttata agaggggtat
     2761 tgatacttta cttactttac gaagtcagtt aatacaaacc ttagtatctt tcaaagaaga
     2821 attatgcaga gaagatttta actcaatccc cttcataaac gctgatggtt atcatagtaa
     2881 gacgattgct tattctattt ggcatatttt ccgcattgaa gatatagttg tgcatacagt
     2941 gataaatgaa gatgaacaag tattttttgc aggcaattat caagagcgta tcaattcacc
     3001 tatcattaca acaggaaatg aactcatgaa acagcagatt gcagactttt caaaacagct
     3061 taatctggaa gaattatatt tatatatttt cgaagtatgg gaaagcactg agaaaatgct
     3121 tgaacggtta tcttatgatg aattgaaaag aaaaataccg aaagaaagaa aaggatactt
     3181 agaatcgttg aatgtagtaa acgacaatga aaaagcaatt tggctgattg attattggtg
     3241 taataaagat atttgtggac taattcaaat gccgttttca agacattgga ttatgcacac
     3301 agaagcctgt ttgcgtataa agaacaagat acattcatag gcaaaaattt cagtttgtag
     3361 aactaaggaa attggaattt aaggaggaat aaagtatggt tagtgatttt ataagagcag
     3421 catttccatg gattttaatg ggtttatttg tagcaatcag ttgttccctt atgagtaaaa
     3481 aagagaaata aattcctgtt tgtatttata aaagagaaaa aatgagaaaa gtagttttat
     3541 ttattgccat gagccttgac ggatatattg cggacggtaa tggtggagta gcttggctaa
     3601 atggtcatgg aaatgacaat gaaaacatcg acacttatac agaatttacc aaagatatag
     3661 atactgtctt aatggggtgg aatacttctc atcaggttgt tactgaactt tctccgcaag
     3721 aatgggtata taataaattt acaacttatg tattaacaca taaagagtgc aattcttccc
     3781 aacaaattca ttttacaagt gaaaatcctg ttttattatt ggaacggcta aaacaagaag
     3841 caggaaagga tatttggatt tgtggcggag caaatcttgt tcagcagttg gtaagcaaaa
     3901 atatgattga taaatattac atttctgtta ttcctactct gttaggaaat ggggttcgtc
     3961 ttttgggcaa catagacagg gaaataaagt taaggctttg taaaacacag acttataacg
     4021 gaataaccga tttgatttat atgcgtagat aactagagag aaacagaaaa gctgttaaac
     4081 cgacggagcg atccgaccac gttggcggga aaatccttaa gatttttccg ccatgcttgc
     4141 aagggggcgg ttcgcttcgg cggttcggcg agcttgcgag ccgttcaaag cccccgttat
     4201 ctaacaaggg gggcacaaaa aacaagaaac actatacact aacaagcgga aaacgctgta
     4261 tttataaggc aaaaccccat ttttacagta gtgatataaa ttgtacctat atcactatga
     4321 aaattgaata gtaaatatga gaaagacagt tgatttcaaa aaataggctc tttgtcaata
     4381 aggactgatg ggttgtacaa aatc
//
LOCUS       DAFEPQ010000218         2999 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.guided.153, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000218
VERSION     DAFEPQ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.guided.153"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            245..1213
                     /locus_tag="LP543_002947"
     CDS             245..1213
                     /locus_tag="LP543_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBL2323018.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="LP543_002948"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="LP543_002948"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBL2323019.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="LP543_002949"
     CDS             2243..2851
                     /locus_tag="LP543_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBL2323020.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          894 a          574 c          722 g          809 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga ggttctgttg caaagtttta aataaagaat aaaatccct
//
LOCUS       DAFEPQ010000219         2406 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.guided.165, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000219
VERSION     DAFEPQ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.guided.165"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            61..312
                     /locus_tag="LP543_002950"
     CDS             61..312
                     /locus_tag="LP543_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2323021.1"
                     /translation="MTVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHA
                     GMNYAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            526..1221
                     /gene="vanR"
                     /locus_tag="LP543_002951"
     CDS             526..1221
                     /gene="vanR"
                     /locus_tag="LP543_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBL2323022.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1199..2353
                     /locus_tag="LP543_002952"
     CDS             1199..2353
                     /locus_tag="LP543_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBL2323023.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          821 a          430 c          513 g          642 t
ORIGIN      
        1 atttgatagt agatttaaaa ctttgcaaca gaaccagata atccatacag ccaattctta
       61 attactgtaa tggctggtgt taaccaatta gagcgagatc ttattcggat gagacaacgt
      121 gaagggattg aattggctaa gaaagaagga aagtttaaag gtcgattaaa gaagtatcat
      181 aaaaatcacg caggaatgaa ttatgcggta aagctatata aagaaggaaa tatgactgta
      241 aatcaaattt gtgaaattac taatgtatct agggcttcat tatacaggaa attatcagaa
      301 gtgaataatt agccattctg tattccgcta atgggcaata tttttaaaga agaaaaggaa
      361 actataaaat attaacagcc tcctagcgat gccgaaaagc cctttgataa aaaaagaatc
      421 atcatcttaa gaaattctta gtcatttatt atgtaaatgc ttataaattc ggccctataa
      481 tctgataaat tattaagggc aaacttatgt gaaagggtga taactatgag cgataaaata
      541 cttattgtgg atgatgaaca tgaaattgcc gatttggttg aattatactt aaaaaacgag
      601 aattatacgg ttttcaaata ctataccgcc aaagaagcat tggaatgtat agacaagtct
      661 gagattgacc ttgccatatt ggacatcatg cttcccggca caagcggcct tactatctgt
      721 caaaaaataa gggacaagca cacctatccg attatcatgc tgaccgggaa agatacagag
      781 gtagataaaa ttacagggtt aacaatcggc gcggatgatt atataacgaa gccctttcgc
      841 ccactggagt taattgctcg ggtaaaggcc cagttgcgcc gatacaaaaa attcagtgga
      901 gtaaaggagc agaacgaaaa tgttatcgtc cactccggcc ttgtcattaa tgttaacacc
      961 catgagtgtt atctgaacga gaagcagtta tcccttactc ccaccgagtt ttcaatactg
     1021 cgaatcctct gtgaaaacaa ggggaatgtg gttagctccg agctgctatt tcatgagata
     1081 tggggcgacg aatatttcag caagagcaac aacaccatca ccgtgcatat ccggcatttg
     1141 cgcgaaaaaa tgaacgacac cattgataat ccgaaatata taaaaacggt atggggggtt
     1201 ggttataaaa ttgaaaaata aaaaaaacga ctattccaaa ctagaacgaa aactttacat
     1261 gtatatcgtt gcaattgttg tggtagcaat tgtattcgtg ttgtatattc gttcaatgat
     1321 ccgagggaaa cttggggatt ggatcttaag tattttggaa aacaaatatg acttaaatca
     1381 cctggacgcg atgaaattat atcaatattc catacggaac aatatagata tctttattta
     1441 tgtggcgatt gtcattagta ttcttattct atgtcgcgtc atgctttcaa aattcgcaaa
     1501 atactttgac gagataaata ccggcattga tgtacttatt cagaacgaag ataaacaaat
     1561 tgagctttct gcggaaatgg atgttatgga acaaaagctc aacacattaa aacggactct
     1621 ggaaaagcga gagcaggatg caaagctggc cgaacaaaga aaaaatgacg ttgttatgta
     1681 cttggcgcac gatattaaaa cgccccttac atccattatc ggttatttga gcctgcttga
     1741 cgaggctcca gacatgccgg tagatcaaaa ggcaaagtat gtgcatatca cgttggacaa
     1801 agcgtatcga ctcgaacagc taatcgacga gttttttgag attacacggt ataacctaca
     1861 aacgataacg ctaacaaaaa cgcacataga cctatactat atgctggtgc agatgaccga
     1921 tgaattttat cctcagcttt ccgcacatgg aaaacaggcg gttattcacg cccccgagga
     1981 tctgaccgtg tccggcgacc ctgataaact cgcgagagtc tttaacaaca ttttgaaaaa
     2041 cgccgctgca tacagtgagg ataacagcat cattgacatt accgcgggcc tctccgggga
     2101 tgtggtgtca atcgaattca agaacactgg aagcatccca aaagataagc tagctgccat
     2161 atttgaaaag ttctataggc tggacaatgc tcgttcttcc gatacgggtg gcgcgggact
     2221 tggattggcg attgcaaaag aaattattgt tcagcatgga gggcagattt acgcggaaag
     2281 caatgataac tatacgacgt ttagggtaga gcttccagcg atgccagact tggttgataa
     2341 aaggaggtcc taagagatgt atataatttt ggctctttgt caaatgatgt tggtaaagag
     2401 aaatct
//
LOCUS       DAFEPQ010000220         2353 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.guided.167, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000220
VERSION     DAFEPQ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.guided.167"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            109..192
                     /locus_tag="LP543_002953"
     CDS             109..192
                     /locus_tag="LP543_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBL2323024.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            317..1054
                     /gene="erm(B)"
                     /locus_tag="LP543_002954"
     CDS             317..1054
                     /gene="erm(B)"
                     /locus_tag="LP543_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBL2323025.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1224..1484
                     /locus_tag="LP543_002955"
                     /pseudo
     CDS             1224..1484
                     /locus_tag="LP543_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1647..2312
                     /locus_tag="LP543_002956"
                     /pseudo
     CDS             1647..2312
                     /locus_tag="LP543_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
BASE COUNT          855 a          377 c          407 g          714 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccatt ttgtgtataa taggaattga
       61 agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc
      121 caaatgcgta atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac
      181 gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa
      241 cagataacta aaattacaaa caaatcgttt aacttctgta tttatttaca gatgtaataa
      301 cttcaggagt gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
      361 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
      421 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
      481 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaacat
      541 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
      601 taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa aagtggtttt
      661 tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
      721 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
      781 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
      841 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
      901 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
      961 gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt
     1021 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tttaaatttg
     1081 gaaagttaca cgttactaaa gggaatggag ataaattatt agatatacta ctgacagctt
     1141 ccaagaagct aaagaggtcc ctagcgccta cggggaattt gtatcgataa ggggtacaaa
     1201 ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc taagtgtggc aacaatattg
     1261 tattaaaaaa atcgttttat ggttgttcaa attatcctga atgtaagttt actttagctg
     1321 aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa agaattactg gagggaaaag
     1381 aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc ctacaatgcc gttgtaaaaa
     1441 ttggggaaaa gggatatatt gattttatct ctttttcaaa ataaacatgg ggctgaccca
     1501 aaagtagctc ctatatgaaa aatagaggtc tttccattta gatagtagtg gaaagacctc
     1561 tatttatctt ttgaaagtga atacaaaaat aaagcacact ctgctataat ataggtgatt
     1621 aatccatttg agagaggtgc tttatcatgt atcaaaatta taccataaac caactttgtt
     1681 tgcctataga tcttgaaatt aaattagaag aaaatgattt tgctcatgcc attgtgcagt
     1741 ttgttgactc tattcccgat gaagtctttc ttccttatta tcaatctatg ggtagacccc
     1801 aatatcatcc tcgtatgcta ctttccatta ttctttgtgc ctatattcaa ggggtttatt
     1861 cgggccgcca aatacaaaat atgctgatag atagtattcg gatgcgctac ttatctcaag
     1921 agcaattccc taactttcgt actattaatc gttttcgcgt tcatccgatt atgaacgata
     1981 ttctagatca ttcatttgtt caatttcgtg agctactggt tcaaagtggc ttaatcagtg
     2041 gcagtgctct atacattgat ggtacaaaaa ttgaagcgga tgcgaataaa tactctttcg
     2101 tttggaaaaa aagtatttta aagtataagg atagccttga tcaaaaagcc cttgaaaact
     2161 accaacaaat ggtggaaaca aaaatcttac ctgagttaat agatgattta aaggatgaac
     2221 tttctataga agagattgaa aggattcgtc aatctttgga agaaaaggaa aatcaattaa
     2281 ttgaagcgat tgaccaaacc gaaacagtag aagggggctg acccattatt aataaggagt
     2341 ggacaacatg att
//
LOCUS       DAFEPQ010000221         1463 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR46232_1
            SAMN20572162-rid13348383.guided.180, whole genome shotgun sequence.
ACCESSION   DAFEPQ010000221
VERSION     DAFEPQ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572162
            Sequence Read Archive: SRR16917197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176920.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176920.1
            Genome Coverage       :: 293x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:05:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,823
            CDSs (with protein)               :: 2,823
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 34 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572162-rid13348383.guided.180"
                     /isolate="PR46232_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-02"
                     /collected_by="University of Michigan"
     gene            <1..1348
                     /locus_tag="LP543_002957"
     CDS             <1..1348
                     /locus_tag="LP543_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HBL2323026.1"
                     /translation="DFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQI
                     YKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKY
                     LIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNT
                     NLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLR
                     QMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFE
                     GKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGE
                     DILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          622 a          117 c          245 g          479 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga
      421 ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata
      481 tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg
      541 ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa aatttagtac
      601 taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa atacaaattt
      661 agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg aattatctat
      721 actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt ctactatgtc
      781 agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac aaatgcacgg
      841 tttagattat acagatatta gtgaatgtac tattgataat aaacaaaatg tattagaaga
      901 gtatatattg ttgcgtgaaa ctatttataa tgatttaact gatatagaaa aagattatat
      961 agaaagtttt atggaaagac taaatgcaac aacagttttt gagggtaaaa agtgtttatg
     1021 ccataatgat tttagttgta atcatctatt gttagatggc aataatagat taactggaat
     1081 aattgatttt ggagattctg gaattataga tgaatattgt gattttatat acttacttga
     1141 agatagtgaa gaagaaatag gaacaaattt tggagaagat atattaagaa tgtatggaaa
     1201 tatagatatt gagaaagcaa aagaatatca agatatagtt gaagaatatt atcctattga
     1261 aactattgtt tatggaatta aaaatattaa acaggaattt atcgaaaatg gtagaaaaga
     1321 aatttataaa aggacttata aagattgatt atataatata tgaaaagcta ttataaaaga
     1381 cattagtatt aaatagttta aaaaaatgaa aaataataaa ggaagtgagt caagtccaga
     1441 ctcctgtgta aaatgctata caa
//
